LOCUS       DBNGZA010000001         2953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2120975, whole genome shotgun sequence.
ACCESSION   DBNGZA010000001 DBNGZA010000000
VERSION     DBNGZA010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2120975"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC16FU_00005"
     CDS             <1..347
                     /locus_tag="AC16FU_00005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR2992797.1"
                     /translation="YTGLCLVAAPWGAASSGWECTLVKFRALTARAIHEYIASGEPFD
                     KAGAYGIQGLGGALVEGIEGDYENVIGLPVATFAFLLEQLRLTGPPNASVPGAPHGEL
                     LADRGIPGEGSG"
     gene            344..1102
                     /locus_tag="AC16FU_00010"
     CDS             344..1102
                     /locus_tag="AC16FU_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2992798.1"
                     /translation="MTSIGQFFKEKREALGLSLEEMAERTRLQVSYLAALEGDRLDEL
                     PGRPYYRLFLRTYAASLGLNFDQIAREFPETEITAEIRLPPAQTVKQRARRAGIAVAS
                     VVVFAGILFFFLRYGRFSTTGPGQVEKSATETKSAGPKVAPPPARESTEVALQPPLVL
                     RLEGLEDTGVRVVADRDTLFNGLLKKAEVEQWFAQDSFIITLGKAWAVTAFVNEHKLK
                     RLGRPGEPRYRLVIRADNYERLVDSTALSEPGQE"
     gene            1113..>2953
                     /locus_tag="AC16FU_00015"
     CDS             1113..>2953
                     /locus_tag="AC16FU_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR2992799.1"
                     /translation="MYLSRVEIFGFKSFPQRTVLAFDGGITAIVGPNGCGKTNILDAI
                     RWSMGEQRPTVLRGTKMDEVIFNGSRSLKALGLAEVTLSIQNDRGVLPSEYEEVAVTR
                     RLLRTGESEYLLNQIPCRLKDITDLFLDTGMGAHAYSVIHQEMVDLILSSKSSDRREL
                     FEEAAGVAKYKQRRTEALRKLDTTQADLLRLSDLSAEVEKQVTSLKRQVKKAERHQRY
                     TAEIKTLELLRSRHEYFQLRRRRDVAAQEARELEGQKQTVSADLATLELSLENHRLQE
                     TEAEKRKQGAERALLEFRESVRTAEQELLLARERREQAQKDTERLQSEIGELEEKEKA
                     LAGEVADLKTRGDYLARTMKKKETDLEGLQTYLETLRGETARHRHQLEGLRQSLLELE
                     ASLGEVKGDLAGRESRRQEWSDRRHHLQQEETKQNQKLQALVTRSEEVKKELAQKREA
                     LERKQKQQAGLEAQVQAAREELVRAEAGFAQLKNEQAVAVSQRDFWRQVVEANEGYPS
                     GVRTLLQKPRPVKGVLDTLANLLTVEARYARAVEAALGEAAGCLICRDAQAVQKAIAY
                     LDEQGAGRAFFLPLDYFSNGQKVPADSKKDFPRKGVCGWAVDFVRCAP"
BASE COUNT          625 a          860 c          960 g          508 t
ORIGIN      
        1 tatacaccgg tttatgttta gtcgcggcgc cttggggcgc cgcctcctcc ggctgggaat
       61 gcaccctggt gaagttccgg gcgctcaccg cgcgtgcgat tcacgagtac atcgcttccg
      121 gagaaccttt cgataaagcc ggcgcctatg gcatccaggg tctgggcggg gcgctggtgg
      181 agggaattga aggcgactac gaaaacgtga tcggcctgcc cgtggcgacg ttcgcttttc
      241 tcctggagca gttgcgcctg accggtccgc caaatgcttc agttcccggc gcaccacacg
      301 gggagctgct tgccgaccgg gggataccag gcgaggggtc tggttgacga gcatcggcca
      361 gtttttcaaa gaaaagcggg aggcgctggg actttcgctg gaggagatgg ccgagcggac
      421 ccggttgcag gtgagttact tagccgctct ggaaggcgac cggctggacg aactgcccgg
      481 ccggccctac taccgtttgt tcctgcggac ctacgccgcc agtctgggct taaactttga
      541 ccagattgcc cgggaatttc cagagaccga gatcaccgcc gaaatccgtt tgccgcccgc
      601 gcaaaccgtc aagcaacgcg cccggcgagc tggaatcgca gtcgccagcg tggtggtgtt
      661 cgcaggcatc cttttcttct tcctgcgcta cggccgcttc agcaccaccg gacccggtca
      721 agtggagaaa tcggctaccg aaactaaatc ggccggaccg aaagtggcac ctcctcccgc
      781 gcgggaatcg actgaggtgg cgctccagcc gcctttggtt ctccggctgg aaggtctgga
      841 agacaccggc gtgcgggtgg tggctgatcg cgatacgttg tttaacgggc ttttgaaaaa
      901 agcggaggtc gagcagtggt ttgcgcagga tagtttcatt atcaccctgg gcaaggcctg
      961 ggccgtgacg gcctttgtca acgagcataa gctgaagcgg ctgggacggc ccggggagcc
     1021 gcggtaccga ttggtcatcc gggcggacaa ctatgaacgt ctggtggact caaccgcgct
     1081 ttcggaaccc ggacaagagt gagattcttc cagtgtatct ttcgcgcgta gaaattttcg
     1141 gcttcaagtc gttcccccaa aggaccgtcc tggccttcga cggcggcatc accgccatcg
     1201 tcgggcccaa cggctgcggc aaaaccaaca ttctggacgc catccgctgg tcgatggggg
     1261 agcagcgccc caccgttctc cgcgggacga agatggacga ggtgattttc aacgggtcgc
     1321 ggagtctgaa ggctttgggt ctggcggaag tgaccctttc gattcaaaac gaccgcggcg
     1381 tattgccctc cgaatacgaa gaagtggccg tcacgcggcg gcttttgcgc accggcgaga
     1441 gcgaatacct cctgaaccag attccctgcc ggctcaaaga catcaccgac ctctttctgg
     1501 acaccggcat gggggcccat gcctactcgg tcattcacca ggagatggtt gacctgatcc
     1561 tctcctccaa gtccagtgac cgccgggaac tgttcgagga agccgcggga gtcgccaagt
     1621 ataagcaaag gcgtactgaa gccctgcgga agttggatac cactcaggcc gatctcctgc
     1681 ggctttccga tctctccgcc gaagtggaaa agcaggtcac ttccctcaaa cggcaggtaa
     1741 agaaggcgga gcgccaccag cgctacaccg ctgaaatcaa aacgctggag ctcctgcgca
     1801 gccggcatga gtatttccag ctgcgtcgcc ggcgggacgt tgccgcccag gaagcccggg
     1861 aactcgaagg tcaaaagcaa acagtatcag ccgatctggc aaccctggag ctttccctgg
     1921 aaaatcaccg cctgcaggag acggaggcgg aaaagcgcaa gcagggggcc gagcgcgcgc
     1981 tcctggaatt ccgtgagagc gtgcggaccg ccgaacagga actcctcctc gcccgggagc
     2041 gccgggagca ggcccaaaag gatactgaac gactgcaaag cgagatcggt gaacttgagg
     2101 aaaaggagaa ggcgctggcc ggtgaggtgg ccgatctcaa aacccggggc gactacctcg
     2161 ctcgcacgat gaaaaagaaa gaaaccgacc tggaaggtct ccagacgtac ctcgaaacgt
     2221 tgcgggggga gacggcccgc caccggcacc agctggaagg tttacggcag tcgcttttgg
     2281 agctggaagc ctcgctgggt gaggtgaaag gagacctggc cgggcgggag agccgccgcc
     2341 aagaatggag tgaccggcgg caccatctgc agcaagaaga aacgaaacag aaccaaaagc
     2401 tgcaggcgct ggtcacccgg tcggaggaag tcaaaaaaga gctggcgcag aaacgagagg
     2461 ctctggagag aaaacagaag cagcaggccg ggcttgaggc gcaggtgcag gccgcgcggg
     2521 aagaactggt gcgggccgaa gcggggttcg cgcagctgaa aaacgagcag gcggtggcgg
     2581 tttcgcaacg cgatttctgg cggcaggtgg tggaagccaa cgaagggtac ccctccgggg
     2641 tacgcaccct tctgcaaaag ccgcggccgg tgaaaggggt cctggatact ctggccaatc
     2701 tcctcacggt cgaggcccgc tatgcgcgcg ccgtcgaagc ggccttgggc gaggccgcgg
     2761 ggtgcctcat ctgccgggat gcccaagcgg tacagaaagc catcgcctac ctagatgaac
     2821 agggggccgg acgcgccttt ttcctgccgc tcgactattt ctccaacggc caaaaggtac
     2881 ccgccgactc gaaaaaagat ttcccgcgca agggggtctg cggttgggcg gttgatttcg
     2941 tccgctgtgc gcc
//
LOCUS       DBNGZA010000002         2156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_742541, whole genome shotgun sequence.
ACCESSION   DBNGZA010000002 DBNGZA010000000
VERSION     DBNGZA010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_742541"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..>2156
                     /locus_tag="AC16FU_00020"
     CDS             503..>2156
                     /locus_tag="AC16FU_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992800.1"
                     /translation="MLCFTSVANARGKEGSKNTALTTVDLPCTQVRVHRVGSLWHTVT
                     NYGILGTFNNPNVRDPESGLPAPSCEFPGGSGTEYLFMGALWVGGVVGADTLVSTGHD
                     GWLGVSELFPDPCPQGQMQRILSGSPADREYVALFTDTVTLSSLVDPDQFSGRPHKPL
                     NIQITQRTRSWATPPFDRFIIYENWIKNIGAGPISSAFAAIYWDGDVWPRRLLSQGFK
                     DDVTGFIDSLKLAWLADQDGDGESGVFREKRNTKDVMSVKILGGSVPSESVSYNWWIS
                     EGTNPVDQDWGPQRLPGRRNYSGGYGTPEGDVGKYRYMSEGEKDYDQLTLELPHPGWI
                     PPPKNADSLVDGFDTRFLLSVGPFDIAPGESVQVGFAVVAGKDFHVGANDFRNTFPPG
                     NFSGFKDSATVAAFYSLLNFDSLVATVQLAQATYASNYRLPPPGPPEGLRVDTWTDST
                     VTLGWNPSGIVDVFGYNVYRSTDSTFAGPPLNATLLPTNGFVDTTVTRGVLYFYVVSA
                     VDSGLGEGKKSLPVSVLAGQPKIPQSLSAVGGRGEVTGFWTPN"
BASE COUNT          468 a          525 c          609 g          554 t
ORIGIN      
        1 tggtcatccc gctgacctac agcatttcca ccggcattgg ggtgggtttt gtcgcctaca
       61 cgcttttgaa attactttcg ggacggccca aagaagtcca ctggctcctc tatttagtct
      121 cgctcgcctt cctcctcgat tttgctttcc gcgtcacccg aaatactgga taaagactct
      181 ccgataaggg acaaaaatca gagtttctct tgttatggtg acgaaaaata gtcgttcttt
      241 atctcgttga cgtttagctg gttaacgagc acggatttca gttctgattc aggtctacaa
      301 gttttcgccg tttctcttca gttttatttc aggagcctat cttaacctct tgattgacat
      361 tgaggatttt tttcaaaatc gccttaattc tggcatctga tttgactcct gtaattggaa
      421 gaggatatat taaagcggaa agggggaaaa gatgccgagg acggcgcagg tcgttgtcca
      481 gtctttaata gggggaataa ttgtgctttg tttcacttca gtggccaatg cgcggggaaa
      541 agaggggtct aaaaatactg ctctgaccac ggttgatctg ccctgcaccc aggtgcgcgt
      601 tcaccgggtt ggttcgctct ggcatacggt aaccaactac ggaattctgg ggacctttaa
      661 taatcctaat gtgcgtgacc cggagagtgg cttaccagct ccttcctgtg aatttcccgg
      721 cgggtcgggc acagaatatc ttttcatggg agcgctctgg gtgggaggtg tggttggtgc
      781 cgatacactg gtcagcacgg gacacgatgg ttggctgggc gtttccgaac ttttccccga
      841 cccctgcccg cagggccaaa tgcagcgaat cctttctggt tcccccgctg accgggagta
      901 cgttgctctc ttcaccgaca cggttacact atcatccctt gttgatcccg accaattcag
      961 tggtcgtccc cacaaacctc tgaacattca gattacccag cgcacgcgaa gctgggctac
     1021 tccgccattc gacaggttca tcatttacga gaattggatt aaaaatatcg gggctgggcc
     1081 gattagcagc gcgttcgcgg caatttactg ggatggggat gtgtggccaa ggcggttact
     1141 atctcaaggt tttaaagatg atgtcaccgg ctttattgat tcccttaaat tggcctggtt
     1201 ggcggatcaa gatggagatg gagaaagcgg cgtcttcaga gagaaacgga atacaaaaga
     1261 tgtcatgagc gtaaaaattc tgggcggcag tgttccgtcg gagagtgtct cgtacaactg
     1321 gtggatttcg gaggggacaa accccgtgga tcaggattgg gggccacagc ggttacccgg
     1381 caggcgtaat tacagcggtg gatatgggac acccgaagga gacgtgggaa aataccggta
     1441 tatgagtgaa ggcgagaaag attacgacca gttaacattg gaattacccc accccggctg
     1501 gattcctccg cccaaaaatg cggacagtct cgttgatggt tttgataccc gtttccttct
     1561 cagcgttggg ccgtttgaca ttgctccggg cgagtcggtt caggtggggt ttgcggttgt
     1621 ggcgggaaag gattttcacg tgggcgccaa cgactttcgg aatacgtttc ctcccggaaa
     1681 tttcagtggc ttcaaagatt cggcgacggt ggcggcgttc tacagcctgc tgaatttcga
     1741 ttctcttgtc gcaacagtgc aactggcgca ggccacgtat gcttccaatt accggctgcc
     1801 gccgcccggg ccgccggaag gattgcgggt cgatacctgg acggacagca cggtcacgct
     1861 gggctggaat ccgtcgggca tagtggacgt tttcggctac aacgtttacc ggagcacaga
     1921 ttcgacattc gcgggtccgc cgctcaatgc gacattactc ccgacgaatg gtttcgttga
     1981 tacgacggtg acgcgagggg tgctctactt ctacgtcgtt tcagccgttg acagcggatt
     2041 gggtgaaggc aaaaagtcac ttccggtatc ggtgctggcg ggccagccca aaattccgca
     2101 aagtttatct gcggtcggcg gcaggggaga ggtaacgggt ttctggacac ccaatt
//
LOCUS       DBNGZA010000003         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2545364, whole genome shotgun sequence.
ACCESSION   DBNGZA010000003 DBNGZA010000000
VERSION     DBNGZA010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2545364"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC16FU_00025"
     CDS             complement(<1..895)
                     /locus_tag="AC16FU_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019737.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HIR2992801.1"
                     /translation="MSRRSQTLLILAGLLWMVSVPAGAKALPSDVTLLSADDRGVVLT
                     YAVPQTEITPIETPNGKFQRIYLPNCPNSTFPGEPELPARIILLGVPPEAEITAEVVT
                     FDFDERAGIDVCPVPGVSGGDQKLGTPIAKYQKSQSAYALAGFFPSGVVAVEGPFFLR
                     HQRAVRVKLFPVQYNPQTRTLRVARQITVRVNFSRGGTTPSQAALPSGDSYEPIYRAG
                     LLNYEQARGWRVTPPPQSLAQAAQVDSFSRSPDWYKITVRDNAIYKIDAPLLAAAGVN
                     LATLDPRTIRIFSGDGRALLPF"
     gene            complement(1419..2369)
                     /locus_tag="AC16FU_00030"
     CDS             complement(1419..2369)
                     /locus_tag="AC16FU_00030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR2992802.1"
                     /translation="MTPRVKEILGWYGSENPGVRANLARIMNHGTLAGTGRFVILPVD
                     QGFEHGPARSFAPNPAGYDPRYHFQLAIEASCNAYAAPLGFISDGAADYAGEIPLILK
                     LNNADTLSKAGDPVQAVTGSVRDALRLGCAAVGFTIYPGSAYAREMYQQVRELAEEAK
                     SFGLAVVVWSYPRGSGLSKEGETAIDVVAYAAHIAAQLGANIIKVKPPTAHIEQAEAK
                     KVYETQRVPIGTLTERVRHVVQSTFNGRRIVIFSGGPAKTDAELLEEVRAIKAGGGFG
                     SIIGRNSFQRPKPEALELLRKIMALYKDEPVRQLVQTALV"
     gene            complement(2423..>3520)
                     /locus_tag="AC16FU_00035"
     CDS             complement(2423..>3520)
                     /locus_tag="AC16FU_00035"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR2992803.1"
                     /translation="VFFRALLKPGDIVLANSHFDTTRANVEHRGAIAQDCLVAEGWDL
                     DSDFPFKGNIDLAQVEETIRTHGKEKIQLCLLTITNNAGGGQPVSLENMRSLRALLKP
                     HGIPLFFDAARFAENAYFIKTREAGCRDKSIPEICREMFACADGCLMSAKKDGLANIG
                     AFAAVNDDDLAQGIKNNLVLFEGFPTYGGLAGRDLEAIARGLEEVLDEGYLSFRVGQV
                     AFLGRRLSEMGVPILKPVGGHAVFVDAGRFLPHIPRDEFPGQALVVALYREGGIRAVE
                     IGSLSFGGASPVSGEKHRVIAELVRLAIPRRVYTNAHLDYVVATIETIARKRDSLRGF
                     AITYEPQVLRHFSVKLMEINEKTVRKTAVGV"
BASE COUNT          741 a         1062 c          992 g          725 t
ORIGIN      
        1 tgaagggcaa aagtgcacgg ccatcccccg aaaagatgcg gatcgtacgc gggtcaagcg
       61 tggcgaggtt tacccccgcg gccgcgagca ggggggcatc gattttgtag atggcgttgt
      121 cgcgtacagt gattttgtac cagtcgggcg accgggaaaa agaatcaacc tgagccgctt
      181 gggccaggga ttgaggcggc ggcgtcacac gccaacctct ggcttgttcg taattcaaca
      241 gccccgctcg gtaaataggt tcatatgaat caccagacgg aagagccgcc tgagaagggg
      301 tggtgccgcc acgagagaag ttaacgcgca cggtgatctg ccgggccacg cgcagggtgc
      361 gtgtttgggg attgtactgc accggaaaaa gttttacccg gaccgcccgc tggtgacgca
      421 gaaaaaaagg accttcaacg gccactacgc cggagggaaa aaacccggcc agagcatacg
      481 cactttgtga cttctgatat ttggcaatgg gtgtgcccag tttttgatcg ccgccactca
      541 ctcccggaac cggacagaca tcaatgcccg cccgttcgtc aaagtcaaaa gtgaccactt
      601 cagccgttat ctccgcttcc ggaggaacgc ccaaaaggat gattcgggcc ggcagttccg
      661 gctcgccggg aaaagtcgag ttcgggcaat tggggaggta aatcctctgg aacttgccat
      721 tcggggtttc tatgggagta atctccgtct gcggcacggc gtaggtgagg accactcccc
      781 ggtcatcggc ggagagcaaa gtgacgtccg aaggcaatgc ctttgccccg gctggcaccg
      841 ataccatcca gaggaggcct gccaggatca gtaacgtttg cgagcgtcgc gacataaata
      901 aaaaaaccga gccacaggtt gtaccacacc cgtcttgccc ggtccgttac cggctggtgc
      961 tttcgttttg ccgactcacc cttcgacaga taaagggcta agtctgcgcg tgatacaacc
     1021 tgttaactct ggctcgttat tttcgctcct ggtagtctgt ggctaactta aagcaaatat
     1081 aggctctacg gtcaaaactg tcaagggaga aatcgcaatc cccgctcatt ttggctcttt
     1141 gctgacccgt tgcaaactgc ctgccagcgc aagtcacttt atcttctggt aactgtttgc
     1201 ctgactgcgg cttgctccaa ttttttctct ccgccgacta ctctctattc ctgattgcgc
     1261 tgccgtctga ccaatggttg caagaacttg ccgggaattc caccgcggtg acttgaccga
     1321 gacacagtca gctggcatgt tctttatcgg caataaaaac gacaaaatca aacgccgggg
     1381 gtcctcacca gtcaagggtt gtaaaattac ggggcgattt acaccaatgc cgtttgtacc
     1441 agctgacgta ctggttcgtc tttgtagagc gccatgatct tgcgcaaaag ttccagggct
     1501 tccggcttgg ggcgctgaaa actgttccgg ccgatgatgg agccgaatcc cccgccagcc
     1561 ttgatcgccc gaacctcctc caaaagttcc gcatcggtct tggccggccc cccggagaag
     1621 atgacgatcc tgcggccgtt gaaagtgctt tgcaccacgt gccgcactcg ctcagtcaaa
     1681 gtcccgatcg gaacccgctg ggtctcgtaa accttcttgg cttcggcctg ctcgatgtga
     1741 gccgtcgggg gtttgacctt gatgatattg gcacccaact gcgccgcaat gtgcgcggca
     1801 taggccacca cgtcaatggc ggtctccccc tctttagaga gtcccgaacc gcgggggtag
     1861 gaccaaacca ccaccgccag accaaaggac ttggcctctt cggccaactc ccgcacctgc
     1921 tggtacatct cccgggcgta ggctgagccg gggtaaatcg taaagccgac agccgcgcac
     1981 cccagccgga gcgcatcccg caccgagccg gttacggctt gcaccgggtc cccagcctta
     2041 ctgagtgtgt ctgcattatt caacttgaga atcaggggaa tttcgcccgc atagtcagcc
     2101 gcaccatcgc tgataaagcc caagggcgcc gcgtaggcat tgcaactggc ctcgatggct
     2161 aactggaagt ggtagcgcgg gtcatacccg gcgggattcg gggcgaaact ccgcgccgga
     2221 ccgtgctcaa aaccttgatc gacggggaga atgacgaatc gcccggttcc agccaacgtc
     2281 ccgtggttca taatacgcgc cagattggcc cgcacgcccg ggttttccga gccataccag
     2341 cccaatattt ctttaactct cggtgtcata tctcttctcc tgaaacgtac gctgcacccg
     2401 gaagttcact cccaccttcg acctacaccc ctaccgcggt ttttcgtaca gttttctcat
     2461 tgatttccat cagcttcacc gagaaatgcc gcagcacctg cggctcgtag gtgatggcga
     2521 aacctcgaag tgagtctctt ttccgggcaa tagtctcgat ggtcgcaacg acgtaatcga
     2581 gatgggcatt cgtgtagacc cggcggggaa ttgccaggcg gactaattcg gcgatgaccc
     2641 ggtgcttttc cccgctcacc ggcgaggcac cgccgaagga gagcgacccg atctcgacgg
     2701 cgcggatgcc gccttcgcgg tagagcgcca ctaccagcgc ctgccccgga aactcgtcac
     2761 gggggatgtg cggcaaaaag cgccccgcat cgacgaacac ggcatggccg cccaccggct
     2821 tgaggatcgg cacacccatc tccgagagcc ggcggccgag aaaggccacc tgcccgacgc
     2881 ggaatgacag atagccctca tccaagactt cttccagccc gcgcgctatg gcttcgagat
     2941 cccggcctgc cagaccgccg taggtgggga agccttcgaa gagcaccagg ttgtttttga
     3001 tcccctgcgc cagatcatca tcgttgactg ctgcgaacgc cccgatattc gccaggccgt
     3061 cttttttggc cgacatcaaa cagccgtcgg cgcaggcgaa catctcccgg cagatttccg
     3121 gaatcgattt gtcccggcag cccgcctcgc gggtcttgat gaagtaggcg ttctcggcaa
     3181 agcgcgccgc atcaaagaaa agcggaatgc cgtggggttt caggagtgcc ctgagcgagc
     3241 gcatattctc aagtgacacc ggctgcccgc cgccggcatt gttggtgatg gtgagcaagc
     3301 agagctggat tttctctttc ccgtgggtcc ggatggtctc ttccacctgc gccagatcaa
     3361 tattcccctt gaagggaaag tcggaatcga ggtcccagcc ctccgcaacc aggcaatcct
     3421 gcgcgatggc cccgcggtgt tcgacattgg cccgcgtcgt gtcgaagtga ctgttggcca
     3481 aaacgatgtc gcccggtttc aggagggctc ggaagaagac
//
LOCUS       DBNGZA010000004         3883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1803592, whole genome shotgun sequence.
ACCESSION   DBNGZA010000004 DBNGZA010000000
VERSION     DBNGZA010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1803592"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..248
                     /gene="rpoZ"
                     /locus_tag="AC16FU_00040"
     CDS             27..248
                     /gene="rpoZ"
                     /locus_tag="AC16FU_00040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR2992804.1"
                     /translation="MQKLIPLDDLEKVTRNRYEAVLVAAKRARSLNAQRKQAEEQAGT
                     DSLASDARRVTAQALEELLKGQLKFVRMQ"
     gene            272..2521
                     /gene="topA"
                     /locus_tag="AC16FU_00045"
     CDS             272..2521
                     /gene="topA"
                     /locus_tag="AC16FU_00045"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR2992805.1"
                     /translation="MAKNLIIVESPTKSKTLVKFLGRDFSVFATKGHVIDLPKSRLGV
                     DLKRNFKPTYVVVPGRKEVLEALAEKAKGVKKIYLAPDPDREGEAIAWHVANRLAILK
                     KPVLRATFNEITKSAVLRGIEKAGKIDLDLVNAQQARRILDRLVGYQVSPLLWKTVHR
                     GLSAGRVQSVALRMICEREEAVKNFVPQEYWTIAATFATKKNEAFIARLVKIDGANPE
                     IPSQAKADEITADIRKKSYAVSSVTVEEKSRNPFPPYITSTLQQDAARRLFFSAAKTM
                     QIAQQLYEGVELGDEGSVGLITYMRTDSTRTAPEAVEMVRSYISHQFGDKYLPKEPRT
                     FKARKGAQEAHEAIRPTLLDYSPLAIKRYLTKDQFKLYELIWSRFIASQMEAARFEVT
                     TVEITGAPYLFRASAQAVKFDGFLAVYQDLKDENGDEEEAKALPPLKESDPLRLSDVT
                     PSQHFTKPPPRFSEATLVKELEANGIGRPSTYAPTIDTLKKRGYVTTEKRVFTPTELG
                     VTVNRILVEHFPDIFNVEFTAEMEAELDRIEEGEDNWVAVLKDFYGPFSEDVKKADAN
                     HRAIQKTTQEKTDEVCEKCGKPMVIRWGKRGRFLACSGYPGCKNTKPLNGEEAVPVEG
                     EKCGTCGAPMLLKTGRFGKFLACSRYPECKTTRPVSTGVPCPEKDCGGTLVERQSKRR
                     KVFYSCSNYPKCKFALWDKPVKQPCPACGAPFLVSKFTKAKGTHLKCVKCNHVVEQPQ
                     AETKVAQPV"
     gene            2567..3451
                     /gene="xerA"
                     /locus_tag="AC16FU_00050"
     CDS             2567..3451
                     /gene="xerA"
                     /locus_tag="AC16FU_00050"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HIR2992806.1"
                     /translation="MSVLEAKRTVSKFLDYLAKERRFSPNTVTAYRNDLVQFLQSTHG
                     ALDFSSRTLEHFLGRLKKQGYDNHSLSRKISTLRSFVRFLQRRMKSVVPASVDFPTIK
                     TQKRVPEFLSVEKTRQLLDSIEVHDLWSARDRAILELFYSTGIRLSELAGLKDHEVDF
                     SRGEITVHGKGAKDRVVPVGQVALGALRTYLALRSNFRKNGRFGYLFINRQGGQLTAR
                     SVARIVVKYGLPYAEGRPLGPHTFRHTFATHLLDRGADIRAVQELLGHRRLSTTQIYT
                     RVTVEKLKKAYDQAHPRA"
     gene            3426..>3883
                     /gene="hslV"
                     /locus_tag="AC16FU_00055"
     CDS             3426..>3883
                     /gene="hslV"
                     /locus_tag="AC16FU_00055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR2992807.1"
                     /translation="MTKHILVPEEQKWRSTTIVGIIHNGKVALGGDGQVTFEDTILKH
                     NAKKLRRLHNGTILAGFAGASADAFALFDRFESKIEEYKGNLPRAAVELAKDWRTDRY
                     LRRLEALLAVLDKEHALIISGNGDVIEPDDKIVCIGSGGAYALAAARALI"
BASE COUNT          888 a         1147 c         1143 g          705 t
ORIGIN      
        1 tctcaatagt aaggagaaaa gagcgtatgc agaaactcat tccattggac gatctggaga
       61 aagtaacccg gaaccgctac gaagcggtgc tggtggcggc caagcgggcg cgcagtttga
      121 acgcccagcg caaacaggcg gaggaacagg cggggaccga cagcctggcc tccgacgccc
      181 ggcgcgtcac cgcgcaggcc ttggaggaac tgctcaaagg ccagctcaag ttcgtgcgga
      241 tgcagtagcg tttgccgata aaggatagta cgtggcgaaa aatttaatca tcgtcgagtc
      301 gccgaccaaa tcgaaaaccc tggtgaaatt tctgggccgg gatttttccg tctttgctac
      361 caaggggcac gtcatcgacc tgcccaaaag ccgcttgggc gtcgatttga agcgcaactt
      421 caaaccgacc tacgtcgtcg tgccggggcg caaagaggtc ttggaggctc tggccgaaaa
      481 ggccaaaggc gtcaaaaaga tttacctggc gcccgacccg gaccgtgagg gggaagcaat
      541 tgcctggcac gtggccaacc ggcttgcgat tctgaagaaa ccggtgttgc gggcgacctt
      601 caacgagatc accaagtcgg cggtcttgcg cgggatcgaa aaggcgggca aaatcgactt
      661 agacctggtc aacgcgcagc aggcgcggcg cattctggac cgcctggttg gctaccaggt
      721 gagcccgctt ttgtggaaga ccgtccaccg ggggctctcc gcggggcgcg tccagtcggt
      781 ggccctgcgg atgatctgcg agcgggaaga ggcggtcaag aattttgtcc cgcaggaata
      841 ctggaccatt gccgcgacgt tcgcgacgaa gaaaaacgaa gcgttcatcg cgcggctggt
      901 gaaaatcgac ggcgccaacc cggagattcc ctcgcaagcc aaagccgatg aaatcaccgc
      961 cgacatccgg aaaaaatcgt atgcggtcag ttccgtcacc gtagaagaaa aatcgcgcaa
     1021 cccattcccg ccctacatca cgagcaccct ccagcaggat gcggccaggc ggctcttctt
     1081 ctcggcggcc aagacgatgc agatcgccca gcagttgtac gaaggagtgg aattgggcga
     1141 cgaaggctcc gtcgggctca tcacctacat gcgcaccgac tccacccgca ccgcgccgga
     1201 agcggttgaa atggtgcgct cctacatcag ccaccagttc ggcgacaagt atttgcccaa
     1261 ggagccgcgc acgttcaaag cgcgcaaggg agcgcaggaa gcgcacgaag cgattcggcc
     1321 caccttgctg gactattcac ctctggccat caagcgttat ttgacgaagg atcagttcaa
     1381 actctacgag ttaatctgga gccgcttcat tgcgtcgcag atggaagcgg cgcgcttcga
     1441 ggtcaccacg gtcgaaatca cgggagcgcc gtatcttttc cgcgccagcg cgcaagcggt
     1501 caagttcgac gggttcctgg cggtctatca ggacttgaaa gatgaaaatg gagatgagga
     1561 agaggcgaag gcgcttcccc ctttgaaaga aagcgacccg ctccgccttt ccgacgtcac
     1621 gccttcccag cacttcacca agccgccgcc gagattctcc gaggcgacgt tggtcaaaga
     1681 attggaggcg aacggcatcg gccggccctc cacctacgcg ccgacgattg acacgctcaa
     1741 aaagcgcggc tacgtcacca ccgaaaagcg ggtcttcacg ccaaccgagc tgggcgtcac
     1801 cgtcaaccgc attttggtcg agcatttccc ggacattttc aacgtggagt tcaccgccga
     1861 aatggaagcc gaactggacc ggatcgaaga gggcgaggac aactgggtgg cggtgctgaa
     1921 agatttctac ggccccttct ccgaagacgt caagaaagcc gacgccaacc accgcgccat
     1981 tcagaaaacg acacaggaaa agaccgacga agtctgcgaa aagtgcggca aaccgatggt
     2041 gatccgctgg gggaagcgcg ggcgcttttt ggcctgctcc ggctatcccg ggtgcaagaa
     2101 cacgaaacct ttgaacggcg aagaagcggt tcccgtcgaa ggcgagaaat gcgggacctg
     2161 cggcgcgccg atgctgctca agaccgggcg cttcggcaag tttttggcct gctcgcgcta
     2221 cccggagtgc aaaacgacgc ggcccgtctc caccggcgtg ccctgtccgg aaaaggactg
     2281 cggcgggacg ttagtggagc ggcagagcaa aaggcgcaag gttttctaca gttgttcgaa
     2341 ttaccccaag tgcaaattcg cgctctggga taagccggtc aaacagccct gcccggcctg
     2401 cggggcgccg ttcctcgttt ccaagttcac gaaagccaaa ggcacgcatc tcaagtgcgt
     2461 caagtgcaat cacgtagtgg agcagccgca ggccgaaact aaagtcgctc agcccgtctg
     2521 atcacgttct ttcctttcat cccttacaac tgcatattag cacctgatgt ccgtgcttga
     2581 ggcgaagcgc acagtcagca agtttctcga ttatttagcc aaggagcgtc gcttttcccc
     2641 taacacggtg acggcctacc gcaacgacct cgttcaattt ctccaatcga cccatggcgc
     2701 gctcgacttc agcagccgga cgctggaaca tttcctcggt cgcctaaaga aacaagggta
     2761 cgacaaccac tccctttcgc ggaagatttc gaccctccgc tcttttgtcc gctttctcca
     2821 gcggcggatg aagagcgtcg tccccgcgtc agtcgatttc cccaccatca aaacccagaa
     2881 gcgggtgccg gaatttttga gcgtcgaaaa aactcgtcaa ctcctcgatt ccatcgaagt
     2941 gcacgacctc tggtccgccc gtgacagagc gatcttagaa ctcttctact ccaccggcat
     3001 ccggctgtcg gagttggccg ggctgaaaga ccatgaagtc gatttcagcc gtggggagat
     3061 caccgtgcac ggcaaaggag ccaaagaccg cgtcgtgccg gtggggcagg tggcgttagg
     3121 ggcgctgcgg acatatcttg ccctgcgcag caattttaga aagaacggac gattcggata
     3181 ccttttcatc aaccggcagg gagggcaact gaccgctcgc agcgtggcgc ggattgtcgt
     3241 caagtacggc ttgccgtatg ctgagggccg tcctttgggc ccccacactt tccggcacac
     3301 ctttgccacg catctgttgg atcgcggggc ggacatccga gcggtgcagg agttgttggg
     3361 acaccggcgg ctttcgacca cgcagattta cacgcgggta accgtggaaa aactgaagaa
     3421 ggcctatgac caagcacatc ctcgtgcctg aagaacaaaa gtggcgctcg accaccatcg
     3481 tcgggataat tcacaacggc aaggtcgcgc tcggcggcga cggccaggtg acctttgagg
     3541 acaccatcct caagcacaac gccaaaaaac tccggcgcct gcataacggg acgattttag
     3601 cgggtttcgc cggggcgtcg gcggatgctt ttgcgctttt cgaccggttc gagagcaaaa
     3661 tcgaagagta caaaggcaac ctgccccgcg ccgccgtcga attagccaaa gactggcgca
     3721 ccgaccgcta cctgcggcgg ttagaagcgc ttttggccgt tttggacaaa gagcacgcgc
     3781 tcatcatttc cggcaacggg gacgtgattg agccggacga caaaatcgtc tgcatcggct
     3841 ccggcggggc gtatgctttg gccgccgcgc gggcgttgat cca
//
LOCUS       DBNGZA010000005         4825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_637281, whole genome shotgun sequence.
ACCESSION   DBNGZA010000005 DBNGZA010000000
VERSION     DBNGZA010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_637281"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="AC16FU_00060"
     CDS             complement(<1..436)
                     /locus_tag="AC16FU_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2992808.1"
                     /translation="MLRFVVSVVFLCLFLPFSSTSGQVTQLYQINGAAGDSLGYSVAG
                     VGDVNGDGKADFIVGAPAAGGVGSAYIYSGVTGVLLFQKNGAAADDNFGNSVAGTGDV
                     NGDGKDDFIIGAPFADPGGLTNAGSTFVYSGATGLLLFQKDGA"
     gene            751..1674
                     /locus_tag="AC16FU_00065"
     CDS             751..1674
                     /locus_tag="AC16FU_00065"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2992809.1"
                     /translation="MARVSIAKIGKRFNGFEAVKNLSLEIPAGTIYGLLGPNGAGKTT
                     TIRMLLNIIAPDTGTVRINGQLWSEELKNKIGYLPEERGLYRKMKVADLLLFLAEIKG
                     VPRAQAKQRMTPWLARLDLEKWKERKAEELSKGMQQKLQFIATVLHEPELVVLDEPFS
                     GLDPVNTDILKDIVLELRQKGTTVLFSTHVMEQAEKLCDAICLINRGQKVLEGSLGQI
                     KSRFGRNSVRVAVEGDGQFLRQLPGVQKIDDYTNYAELQLSVGAAPQAILQTIAQRAT
                     VKRFEVVEPSLHQIFLEVVRDHPTEKTGEKP"
     gene            1671..2936
                     /locus_tag="AC16FU_00070"
     CDS             1671..2936
                     /locus_tag="AC16FU_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2992810.1"
                     /translation="MKDLWAVLKREFTEATRKKSFLIWTIITPFYMALLFALPSWLAS
                     KKSGPDRPKQIAIIDRTGILRDRLIAALVDTLPNGRKMFQVETLDPQLSPDSTEALCR
                     ARVLEGTLDAYVNIPAEALDSGRAQFYSKSTGDFILPNAIERRLNQALLQYRSRTFGI
                     DSTVAAALTRRADLAALKVTKEGAKTTQFITGYLVALSFVMILMSLILSFGQTLMRGI
                     IEEKNSRVIEVLVSSVRPFQLLMGKVLGLGVAGLFQIVIWVARAVLAFALTGSQGWLG
                     QALTSIPGNLVTLFIIYFILGYFLYSAFFALIGAVCQSEQEAQHLMFPLILFLIMPFI
                     LGFMVSQNPGGSVSTLLSFFPLLTPTLMVMRSYYLMPPVTQIAASIVVLLVSIVLVGW
                     FSAKVFRVGILMYGKRPTLPEVLRWVRYR"
     gene            complement(3009..3614)
                     /locus_tag="AC16FU_00075"
     CDS             complement(3009..3614)
                     /locus_tag="AC16FU_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR2992811.1"
                     /translation="MRTAIFIDGAYLEFTLRESYESVRIDYGLLSEKLAGGIDVLRSY
                     YYNCLPYVGNPPTDEEKTRLADKQRFFDALTRIPRYQVRLGQLAFRGLRTGDNRPIFE
                     QKRVDTLMAVDFVLLCSTHRITHAALLTGDGDFVPAVNVAKEFGVVVSLWYSDRFRAD
                     DELWRVCDERHVIDQQLIDSIRLRSYANGETVRAGRSDSSF"
     gene            complement(3694..3775)
                     /locus_tag="AC16FU_00080"
     tRNA            complement(3694..3775)
                     /locus_tag="AC16FU_00080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3739..3741),aa:Leu,seq:gag)
     gene            complement(3837..4235)
                     /gene="secG"
                     /locus_tag="AC16FU_00085"
     CDS             complement(3837..4235)
                     /gene="secG"
                     /locus_tag="AC16FU_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR2992812.1"
                     /translation="MFTFFLVLHILVCIGLIISILMQSAKGEGLAGAFGGGSISSAVF
                     GGRGAATFLARATTVLAVAFGLTCVSLTLTAPRAGTSGTSAVAKEAAKGGIPAPQPPP
                     PTQVPVTQQPAQPAGQQQPDVNQPPAPKKK"
     gene            complement(4245..>4825)
                     /gene="tpiA"
                     /locus_tag="AC16FU_00090"
     CDS             complement(4245..>4825)
                     /gene="tpiA"
                     /locus_tag="AC16FU_00090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR2992813.1"
                     /translation="GAQNLYPAKEGAFTGEISGAMLIALGCTYVIIGHSERRHILGED
                     DAFINRKLKAAGEVGLIPIFCVGETLDQRQAGKAFDAVWTQLEQGLADIPGEAIQKMV
                     IAYEPIWAIGTGQVATLQQAVEVHRFIRDRLSAHRIDGQAPSILYGGSVNPANAADLL
                     RETEIDGLLVGGASLEAQAFSHIVAAAQVKTR"
BASE COUNT         1017 a         1442 c         1329 g         1037 t
ORIGIN      
        1 cggcgccgtc tttttggaaa aggagtaaac cggtggcgcc ggaatagaca aaagtggagc
       61 cggcattggt aagtccgccc gggtcggcaa aaggagcgcc aattatgaaa tcgtcttttc
      121 catccccatt tacatcccca gtacctgcga ctgagttacc aaaattgtca tcggcggcgg
      181 ccccgttttt ctggaaaagg agaacaccgg tgacaccgga atagatatag gcggagccta
      241 cgccgcccgc agcgggagcg ccaacgataa aatcagcttt tccatcgccg ttcacatctc
      301 ccaccccggc cacggagtag ccaagactgt caccggcggc gccgtttatc tggtaaagct
      361 gcgtaacttg tccagaagtt gaggaaaaag ggaggaacag gcagaggaaa acaacactca
      421 ctacaaagcg caacatacct tcttcccgtg cagacgagac catttgtctc gtattcaccc
      481 ggccttgcgc aaaactctaa gagttaataa accgagccgg gctggaatgt caacaaatcc
      541 tctcgtgtgg aaagggtctc agtccgggat gggccacaac gagtttagtc tcagcctttc
      601 taaccccctg tcgtcccctt tggccggtgg aagaaagtat tctcctattt gtttgccgac
      661 cggcctgcac acttgatttt cgcaacccgg ccacgttcat ttgcgtaaag agtaattgtc
      721 ttttttagcc cgcagaagga cgcgacgttc atggcccggg tctcgatagc aaagatcggc
      781 aaaaggttca acggattcga ggcagtcaag aatctttctc tggagatacc ggcagggacg
      841 atttacggcc tcttaggccc caacggcgcc ggcaaaacca ccaccatccg gatgctttta
      901 aacatcattg cgcccgacac cggcacggta agaatcaacg gacaactctg gtcggaggaa
      961 ttgaagaata aaatcggcta cctgccggaa gagcggggac tctaccggaa gatgaaagtg
     1021 gctgatcttc tcttattcct ggccgaaatc aaaggtgtgc cgcgggctca agcgaagcaa
     1081 cgaatgacgc cctggctggc gcgcctcgac ttagagaaat ggaaagagcg caaggccgaa
     1141 gaactctcca aggggatgca gcagaaactc cagttcatcg ccacggtatt gcacgaaccg
     1201 gaattagtgg tgttagacga acccttctcc ggtttagacc cggtcaatac cgatattctg
     1261 aaagacattg tcctcgaact gcgccagaag ggtaccacgg tccttttctc tacccatgtg
     1321 atggagcagg cggagaaact ctgcgatgct atctgcctca tcaatcgcgg ccagaaagtg
     1381 ttggaaggct ccctggggca gattaaatcg cgcttcggac gcaatagcgt ccgcgtggcc
     1441 gttgagggcg acggccagtt cctgcggcag ttgcccgggg tgcagaagat cgatgactac
     1501 accaactacg ccgaactgca actctccgtc ggcgccgcac cgcaagctat cctgcagacg
     1561 attgcccagc gggctacggt aaaacgcttc gaggtggtgg aaccatccct gcaccagatt
     1621 ttcctcgaag tggtacgcga ccatcccacg gaaaagacgg gagaaaagcc gtgaaagacc
     1681 tgtgggcagt gctcaagcgg gagtttacgg aagccacgcg caagaagagt tttctcatct
     1741 ggacgattat cactcccttc tacatggccc tgctctttgc cctgccgtcg tggctggcat
     1801 ccaagaaatc gggccccgac cgacccaagc agattgccat cattgaccgc accggcatcc
     1861 tccgtgaccg cctgattgcc gctttagttg acaccctccc caacggccgg aaaatgttcc
     1921 aggtcgaaac gcttgatccc cagctttccc cggacagcac ggaggcactt tgccgcgcac
     1981 gcgtgctgga gggcacgttg gacgcttacg ttaacattcc ggccgaggcc ctcgactccg
     2041 gccgggccca gttctattcc aaatccaccg gcgacttcat tctgcctaac gcaatcgaac
     2101 gacgcctgaa tcaggcgctc ctgcagtacc gctcgcgcac cttcgggatc gattccaccg
     2161 ttgcggccgc tttgacccgc cgcgccgacc tcgctgcgct gaaagtcacc aaggaaggtg
     2221 ctaaaacgac gcaattcatt accggctacc tggtggctct ttccttcgtg atgatcctca
     2281 tgtctcttat tttgagtttc gggcagacct tgatgcgggg catcattgag gagaagaact
     2341 caagggtgat cgaagtcctg gtctcctccg tccggccgtt tcagctactc atggggaaag
     2401 tcctggggct gggggtggct ggtctgttcc agattgtcat ctgggtggcg agggcggttt
     2461 tggcttttgc tctgactggc tcccagggct ggctggggca ggccctcacc tcaatccccg
     2521 ggaacctggt gacactcttt attatctatt tcattctcgg gtattttctc tactccgcct
     2581 tcttcgccct catcggagcg gtctgccaga gcgagcagga agcccagcac ctgatgttcc
     2641 ctttaatact gttcttgatc atgccgttca ttctgggctt tatggtttcc cagaatccgg
     2701 gcggctcggt ctcgactttg ctttctttct tcccattact tactcccacc ctgatggtga
     2761 tgcggtcgta ctatcttatg ccaccggtga cgcagattgc cgccagcatt gtcgtgctcc
     2821 tggtctcaat tgttttggtc ggctggtttt ccgccaaagt cttccgcgtg gggattctaa
     2881 tgtacggcaa gcgcccgacc ttgccggaag tgctgcgctg ggtgcgctac cggtagagct
     2941 atttatcctg aagaaactaa cttgctaact tgatggtagt ccggttcgtc cctaagccgg
     3001 acgaaccgtc agaacgaaga atcactgcgc ccggcccgaa cggtctctcc gttcgcgtaa
     3061 gacctcaagc ggatcgaatc gatcaactgc tggtcgatca cgtggcgctc gtcgcagacg
     3121 cgccacagtt cgtcgtcggc gcggaagcgg tcgctgtacc agagcgacac caccacgccg
     3181 aactctttgg ccacgttgac cgccggaacg aaatcgccgt cgccggtgag caaggccgcg
     3241 tgagtgattc ggtgggtgga gcagagcaga acgaaatcga ccgccatcag cgtatcgacc
     3301 cgcttctgct cgaagatcgg ccggttgtcc ccggtgcgta atccccggaa ggccagctgc
     3361 cccagccgca cctggtaacg cggaatccgt gtcagggcat caaagaagcg ctgcttgtcg
     3421 gctaagcgcg tcttttcctc gtcggtcggc ggattgccca cgtagggcag acagttgtag
     3481 tagtaggagc gcaggacgtc gatgcccccg gccaactttt cggagagcag gccgtaatca
     3541 atgcgcaccg actcgtagct ttcccgcagg gtgaattcga ggtaggcgcc gtcaatgaaa
     3601 atcgcagttc gcatagacca ctactcctta aagtgtcagt ttcgttctgt gcaacctcaa
     3661 tcggtctcgt tttctgcctg gacgggcaaa ctatgccgaa ggcgggattc gaacccgcac
     3721 gccttgcggc gccaccccct caagatggtg tgtctgccaa ttccaccact tcggcattta
     3781 tctgatggaa gataaagcaa tgttcccggg cgtttcaccc cacagaaccg cttttttcac
     3841 ttcttcttcg gcgccggggg ctgattcacg tcgggttgtt gttgacccgc cggctgggcc
     3901 ggttgctgcg taacgggaac ctgcgtcggc ggtggcggct gtggcgccgg gatgccgcct
     3961 ttggccgctt ctttggcgac cgcactggtc cccgaggtac cagctcgggg ggcggtcaaa
     4021 gtcaaggaga cgcaggtgag cccgaaggcc accgccaata cggtcgtggc gcgggcgaga
     4081 aaggtggccg cgccccgccc gccgaatacg gcgctggaga tactcccgcc gccgaacgca
     4141 cccgccagcc cttccccttt ggccgactgc atcaaaatcg aaatgattag accaatacaa
     4201 accagaatgt gaagtactaa gaaaaacgtg aacacaactt cctcctacct cgttttcacc
     4261 tgcgccgccg ccacaatatg gctgaacgcc tgcgcctcca gactggcgcc gcccaccaat
     4321 aaaccgtcaa tttcggtttc gcgcaacagg tcggcggcgt tcgcgggatt gaccgacccg
     4381 ccgtagagga tcgatggtgc ctggccgtcg atcctgtggg cggagagccg gtctcgtata
     4441 aaacggtgta cttccaccgc ctgttggaga gtggccacct gaccggtgcc gatggcccag
     4501 atcggttcgt aggcaatgac cattttctga atagcttctc cgggaatgtc ggctaatccc
     4561 tgctccaatt gcgtccacac ggcgtcgaac gcctttcccg cctgtcgctg gtcgagcgtc
     4621 tcccccacgc agaaaatggg tatgaggccg acctcccccg ccgctttgag tttacggttg
     4681 atgaacgcat catcctcacc cagaatatgc cgccgctcgg agtggccgat tatgacgtac
     4741 gtgcaaccga gcgcgatgag catcgctccg gatatttcgc cggtgaacgc cccctctttg
     4801 gccggataga gattctgcgc accca
//
LOCUS       DBNGZA010000006         2897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_531420, whole genome shotgun sequence.
ACCESSION   DBNGZA010000006 DBNGZA010000000
VERSION     DBNGZA010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_531420"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16FU_00095"
     CDS             <1..263
                     /locus_tag="AC16FU_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019721397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2992814.1"
                     /translation="PSKAEARGGVVAFNLPQMHPHDLGTILNEEGIAIRAGHHCCQPL
                     MRKLGVTGTARASFHVYNTRAEVDALVSGLHKAQEIFSGVGT"
     gene            250..714
                     /gene="sufU"
                     /locus_tag="AC16FU_00100"
     CDS             250..714
                     /gene="sufU"
                     /locus_tag="AC16FU_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019912202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR2992815.1"
                     /translation="MSVRDDLYREIILDHFKSPRNRGKLPEPALKVEGANPLCGDEVE
                     LFILEKDGQLADVRIETHGCSISQAAASMMTEAIKGQSLADAEKLAGEFKEMMLGNGS
                     QPMPEDFGDLEALEGVKKYPVRIKCALLAWNTLLEGIKALKEHKPAQQVIME"
     gene            784..1206
                     /locus_tag="AC16FU_00105"
     CDS             784..1206
                     /locus_tag="AC16FU_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR2992816.1"
                     /translation="MSDEIPQDKIENSDVTQPQSPPATSPSPEAQAPLSLASLPPPTP
                     EQIREVIKEIVDPEIGISIVELGLIYGIEVRDHDVYVTATLTSPACPLGEVIKEQVEE
                     RSGSMPGVRYVYFELVWEPPWDPKTMCSEEAKLELGIF"
     gene            complement(1282..>2897)
                     /locus_tag="AC16FU_00110"
     CDS             complement(1282..>2897)
                     /locus_tag="AC16FU_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992817.1"
                     /translation="QVYFGTTPKLGLTFAGGTYDTVFSVKLAGLLTATRYHFRLDPPP
                     KNPGGSRFTNDFVYATLAPKGWTEFLRLPVLIVVYTPIHYRDSDSLSSRNESKPARLS
                     DQDVAALKAQGETFRTFFWRNTKCKLDLVLEYLFIDTARADLEKDLEKYVLTIGKKPE
                     DFAGVIFVDTWNDYLDPAKSAKLYRGQALAGETYGVNGPWKFKDLPYSVISPSHGQNV
                     TYALVHEFHRQIDALFYASGYPEYPSNDPNKGEVSGLFGEYYDCEAFILRNWPSEHWS
                     GLHRGEFVAVRDDNNNGIPDDARVAVNDRTFGSGKSSDTTWTPMKKLLATNGILSGVD
                     ERWGGRVNPPDGRDGYPLTKARSDRPKKTPILDGKLSPGEWEEFATLNDRQLRAKIYF
                     QWDDSALYFAAEMSRPASLHLDIDAGNNGWFYGGDNYRLAVNSKETGATDSVAIYDWS
                     FFPRQPGFWNYEKIRPEELRSACTMGKRKCVIELAIPKNEGTGLRLQAGNEMNIRIGF
                     DWPGNKEGMVTLFEPHRFFGVKLVENNVR"
BASE COUNT          650 a          830 c          790 g          627 t
ORIGIN      
        1 gcccgtcgaa agccgaagcc agaggcggcg tcgtggcgtt caatctgccc cagatgcacc
       61 cgcacgacct gggcaccatc ctgaacgaag agggcatcgc catccgggcc gggcaccact
      121 gctgtcagcc cttgatgcgc aagttaggcg tcaccggcac tgcccgggcc agtttccacg
      181 tctacaatac cagagccgaa gtggatgcgc tcgtttccgg tctccacaag gcgcaggaga
      241 ttttttccgg tgtcggtacg tgacgatctc taccgcgaga tcatcttaga ccacttcaaa
      301 agcccgcgca accgggggaa actgcccgaa ccggccttaa aggtcgaggg cgccaatcct
      361 ctctgcgggg atgaagtgga acttttcatt ctggaaaagg acgggcaact ggccgacgtg
      421 cgcatcgaaa cccacggctg ttccatcagc caggccgcgg cctcgatgat gaccgaggca
      481 atcaaaggtc agagtctcgc ggatgcagaa aaactggcgg gcgagttcaa ggaaatgatg
      541 ttagggaacg ggtcgcagcc gatgcccgaa gatttcggcg accttgaggc gttggagggc
      601 gtcaaaaaat acccggtgcg gatcaagtgc gcgcttttgg cctggaacac ccttctggag
      661 ggtataaagg ctctgaaaga acacaaaccg gctcaacagg tgataatgga atgagcgtgg
      721 acctgttttt cgtagccgag gttacgaacc tcggcgcatt cgatctgaat gggtgaaggt
      781 gaaatgagcg acgagattcc tcaggacaaa atcgaaaata gcgatgtgac ccagcctcaa
      841 tctccaccgg ccacgtcccc ctcgcctgag gcccaggctc ccttatccct cgcctccttg
      901 cctccgccaa cacccgagca gatccgcgag gtgatcaagg aaatcgtcga ccccgaaatt
      961 ggcatttcca tcgtggagtt gggcctgatt tacggcatcg aagtgcgcga ccatgatgtc
     1021 tacgttaccg ccacccttac ttcccccgcc tgcccattgg gggaagtgat aaaggaacag
     1081 gtggaggaga gatccggcag tatgccgggc gtgcgctacg tctatttcga actggtctgg
     1141 gagccgccct gggaccccaa gaccatgtgc tccgaagagg caaaattaga gttggggatt
     1201 ttctaaaaag tcaaaattct tagtataaag ggaggcaggc gcctctcttt atttttttcc
     1261 ttggaatctt cagcaaccaa actatcgaac attattctca accagcttca ccccaaaaaa
     1321 ccggtgcggc tcaaagagcg tgaccattcc ttccttgttc cctggccaat caaagccaat
     1381 cctgatattc atttcattcc ccgcctgcag ccgcaacccc gtgccttcat tcttcggaat
     1441 cgctaattcg atcacgcact tcctctttcc catcgtacag gccgagcgca attcttccgg
     1501 cctaattttc tcgtagttcc agaatcccgg ctgacgcgga aagaaactcc agtcgtaaat
     1561 cgcaactgag tccgtagcgc cggtctcttt cgaattcacg gcaagccggt agttgtcacc
     1621 gccgtagaac cagccgttgt tcccggcatc gatatcgagg tgcaaactgg ccgggcgcga
     1681 catttccgcc gcaaagtaga gcgctgagtc atcccattgg aagtagatct tggcccgcaa
     1741 ctggcggtcg ttcaacgtgg cgaactcttc ccattcgccg ggggagagtt tgccgtcaag
     1801 aattggcgtt ttctttggtc ggtcacttct tgctttggta agcgggtaac catctcgacc
     1861 gtccggagga ttgacgcggc cgccccagcg ttcatcgaca ccggagagga ttccgttggt
     1921 tgccagaagt tttttcattg gtgtccacgt agtatcagaa ctttttcctg aaccaaacgt
     1981 ccgatcatta accgccactc gcgcatcatc cggtatgcca ttgttattgt catcccgcac
     2041 cgcgacgaac tcgccccggt gcaagccgga ccagtgctcc gaaggccaat tgcgcaggat
     2101 gaacgcctcg caatcgtaat actcaccgaa aagaccgctc acctcgccct tattcgggtc
     2161 attggacgga tactctggat acccgctggc gtaaaagagc gcgtcaatct gcctatggaa
     2221 ctcgtgcacc aacgcgtagg tgacgttctg gccgtgagag ggcgaaatga cgctgtacgg
     2281 caggtctttg aatttccacg gcccattgac gccgtacgtc tcccccgcca gcgcctggcc
     2341 ccggtaaagt ttggcgctct ttgccggatc aaggtagtcg ttccaggtgt caacgaaaat
     2401 tacgcccgcg aagtcttccg gcttcttccc gatagtcagc acgtatttct ccaggtcttt
     2461 ctcgagatcg gcgcgtgccg tgtcgatgaa caggtactcc agcaccaggt cgagtttgca
     2521 tttcgtgttg cgccagaaga aggttcggaa ggtctctccc tgcgctttga gggcggcaac
     2581 gtcctgatcc gaaagccgcg ccggtttgct ctcattccgg gaagaaagtg agtcggaatc
     2641 ccgatagtgg atcggtgtgt aaacgacaat taaaaccggt aagcgcaaga actctgtcca
     2701 ccctttagga gccagagtag cgtaaacgaa atcattggtg aatcgggaac ccccgggatt
     2761 tttgggcggt gggtccaggc ggaagtggta gcgcgttgcc gtcaaaagtc cggccagttt
     2821 gacggaaaag acggtatcgt aggtgccccc ggcaaaggtc aagcccagtt tcggcgtggt
     2881 gccgaaatag acctgac
//
LOCUS       DBNGZA010000007         1232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2440284, whole genome shotgun sequence.
ACCESSION   DBNGZA010000007 DBNGZA010000000
VERSION     DBNGZA010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2440284"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC16FU_00115"
     CDS             complement(<1..589)
                     /locus_tag="AC16FU_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2992818.1"
                     /translation="MPSILVVDDEKNILSSLRGALGREGYQVDCAATLADGREKLKEA
                     YDFVLLDIWFPEGSGLELLEEIVAGAPETVVVMMSGHGTIETAVKATKLGAYDFLEKP
                     ISLDRLLVLLRNAATTLTLRALNRRLQAERPWAVAIVGRSPAVQQLLREIEQVGPTAA
                     RVLIQGEHGSGKELVARALHAASGRREMPFVAVNCS"
     gene            complement(592..>1232)
                     /locus_tag="AC16FU_00120"
     CDS             complement(592..>1232)
                     /locus_tag="AC16FU_00120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2992819.1"
                     /translation="VAHEVKNPLTPIAISIADLQRSFEQQRPDFPQILAQSVKTVTEE
                     IEALKRLLQEFSDFAKFPPPRFAPCLLSELLADVKTLYARDVDQGRLTFTGEVREITL
                     SVDAGQIKQALVNLIKNGLEAVNGKGRVTVSVQVSDGSLEIAVADTGPGLTPEQKSNL
                     FTPYFTTKTEGSGLGLTIVQRIVSDHAGQISVDSLPGRGTTFRLLLPLELKR"
BASE COUNT          244 a          370 c          359 g          259 t
ORIGIN      
        1 ccgaacagtt caccgccacg aaaggcattt cccggcgccc actggcggcg tggagcgccc
       61 gggccactaa ttctttaccg ctcccgtgct cgccctgaat taagacccgc gcggcggtgg
      121 ggccgacctg ctcaatttcc cgtaacaatt gttgcactgc cggggagcga ccgacgattg
      181 ccaccgccca ggggcgttcc gcttgcagac ggcgatttag ggcccgcagg gtgagcgtcg
      241 tggctgcgtt ccgtaagagg acgagtagtc ggtcgagcga aatcggcttt tccagaaaat
      301 cgtaagcccc taatttggtg gctttcaccg ccgtttcaat ggtcccgtgt ccggacatca
      361 tgacgaccac cgtctccggc gcaccggcga caatttcttc caaaagttcg aggccgctgc
      421 cctcggggaa ccagatgtcg agcaggacaa agtcgtaggc ctcttttaac ttctcccggc
      481 cgtcggccaa agtcgccgca cagtccacct gatacccctc gcgccccaag gccccccgca
      541 ggctggagag aatatttttt tcgtcgtcaa cgactaagat tgaaggcatc gctacctttt
      601 caactccaga ggcaaaagca ggcggaaggt ggtgccgcgc cccggcagag aatcgacgct
      661 gatctgcccc gcgtggtcac tcacgatccg ctgaacaatg gtcaaaccca ggccgctccc
      721 ctccgttttg gtggtgaagt acggggtgaa gaggttgctt ttttgctccg gggtgaggcc
      781 ggggcccgta tcggccactg cgatctccag cgaaccgtcg ctgacctgca ccgaaacggt
      841 aacgcggcct ttcccattaa ctgcttccag gccatttttg attaagttga cgagcgcctg
      901 cttgatctgt ccggcatcca cgctcaacgt aatctctcgc acttctccgg taaaagtcag
      961 acgcccttgg tccacgtctc gggcatagag cgttttgacg tcggcaagaa gttcggagag
     1021 gaggcagggg gcgaaacggg gcgggggaaa tttggcaaaa tcggagaact cctgcagcag
     1081 gcgcttgagc gcttcgatct cttcggtcac ggtcttgacc gactgggcca gaatttgcgg
     1141 aaaatcgggg cgctgttgtt caaaactgcg ctggagatcg gctatcgata tggcaatcgg
     1201 cgtcaaaggg tttttcactt cgtgggccac ct
//
LOCUS       DBNGZA010000008         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_849734, whole genome shotgun sequence.
ACCESSION   DBNGZA010000008 DBNGZA010000000
VERSION     DBNGZA010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_849734"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="AC16FU_00125"
     CDS             complement(<1..1253)
                     /locus_tag="AC16FU_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR2992820.1"
                     /translation="MHQTQTRPRRAGGEEKVRNLILEKWFHLTTRGTNVRTEVLAGVT
                     TFLTMSYIIFVNPAFLSFSADPHLAGKGLPFAASVTATCLAAGICCLLMGLLTNYPFA
                     LAPGMGLNAIVAYQLVVAQGLSWPDAMGVIVAEGVAITVLVLTGLREAVMNAIPLALK
                     KAISVGIGLFIAFIGLVQAGIVKKSEATLVTLGNLREPPVLIALFGLLVTALFIRKKL
                     RGALFFGIILATGLAIIWNAVSGGKIFTTPGVAILPPSLVELPDFSTVGHVSFAFIAH
                     LGFFTAILIVFSIMLSDFFDTLGTVVGLSSEAGFLDDEGRLPQINRVLFVDSCAAWLG
                     GLTNASSVTTYIESASGISVGGRTGLSVMVVGLLFFASIFFSPLVGIVPKEATAPALV
                     IVGFLMLSIIRDIPFDRFDLAFPAFL"
     gene            complement(1288..>2827)
                     /locus_tag="AC16FU_00130"
     CDS             complement(1288..>2827)
                     /locus_tag="AC16FU_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992821.1"
                     /translation="YEDDFQTVMEEVSGRELDWFFDEWIHTTKTIDYAVARPKGKWVE
                     TDSGKVYRTQLRLSRKGESIMPLDLRVTLKNGQARDFLIPLDESAKPTFAQILPIWPQ
                     VREIRKKYAATVDLPAKPKRIEIDPSGRLADVNPLNNRYPIPKVELQLDNLKYNPQPV
                     DAYLLTARPQIWFNSRDAVQLGLHVTGGYLGFTRQLSAEGVSATRRIKGHFAVKATHP
                     FEALGRLAFWSVSARDYDGRRQFAFELSKQIRNRWGYPPVVEHRLISGFERAYDSRYL
                     FDQSSWATTTVPYAAYLFSGHWPKRWGALRVQFQNESGAKGRFISDPAFNRTSGLVRI
                     AWNVVPNWTLLFRLFGSHYIGARVPQKEFRLNGAEPEQVFDLPLLRSKGTIPNRWQKH
                     FRVVGGGNLRGVENPPLGAENIIARTIEVLPPNLVSLSLLRKIPLAGNFLAGMRNYAF
                     FDDGILMFDGAKDRYFYDLGVGFKFPNIWKNQKFQIDFPIFVGSDRRLEPVKFRWVVG
                     WEVE"
BASE COUNT          690 a          781 c          794 g          562 t
ORIGIN      
        1 agaaaagcag ggaaagccag gtcaaaccgg tcgaacggga tgtcgcggat gatcgaaagc
       61 atcaaaaacc cgacgatgac aagcgccggc gcggtcgctt ccttgggtac tatgccgacg
      121 agcggagaaa agaaaatcga agcgaaaaac aaaaggccga ccaccatgac cgaaagaccc
      181 gtccgcccgc ccaccgaaat tcccgacgcg ctctcgatgt aggtggtaac cgaactggcg
      241 ttggttaagc cgcccagcca ggcggcgcag gaatcgacga agagcacccg gttgatctgc
      301 ggcaagcggc cttcgtcgtc taagaatccc gcttcgctgc ttaaacccac gacggtgccc
      361 agcgtgtcga agaaatcgga aagcataatc gaaaagacaa tcagaatcgc cgtgaagaaa
      421 ccgagatggg caataaaagc aaacgaaacg tgacccacgg tagagaaatc gggcagttcg
      481 accagcgaag gcgggagaat cgccaccccg ggtgtagtga aaattttgcc gccggagacg
      541 gcgttccaaa taattgccag gccggtggcc aggataatgc cgaaaaaaag tgctccacgc
      601 aatttctttc gaatgaaaag cgccgtcacc aaaagaccga agagggcgat caaaaccggc
      661 ggctcgcgca gattgcccag cgtgaccagc gtggcttcgc tctttttgac gattcccgcc
      721 tgcacgagac cgataaaggc aatgaatagc ccgatgccga cggaaattgc tttcttgagc
      781 gccaggggga tggcgttcat caccgcctcg cgcaagccgg tcagaacgag cacggtaatg
      841 gctactccct ccgccacgat gacacccatc gcatccggcc acgaaagccc ctgcgctacg
      901 accaactggt aggccacgat ggcgttcagg cccattccgg gtgccagcgc aaagggatag
      961 ttggtgagaa gtcccatgag aagacagcaa attcctgcgg cgagacaggt ggcggtcacc
     1021 gacgccgcaa aaggcagccc cttcccggcc agatgaggat cagcagaaaa actcaagaat
     1081 gccggattga cgaagatgat ataagacatt gtcagaaacg tcgtcacccc agccagtact
     1141 tcagtgcgga cattcgttcc gcgggtggtt aaatgaaacc acttttccag aattaggttt
     1201 cggacttttt cctctccccc cgctcttctc ggccgggtct gggtttggtg catttgattt
     1261 tcctggctac tgattcctgg ccccccgtta ctctacttcc caccccacaa cccagcggaa
     1321 cttcaccggc tccaagcggc ggtcgctgcc gacgaatatg ggaaagtcga tctgaaattt
     1381 ctggtttttc cagatgttgg ggaatttgaa accgacgccc agatcgtaga aataacggtc
     1441 tttggcgccg tcgaacatga gaatgccgtc gtcaaagaaa gcatagttgc gcattccggc
     1501 cagaaaattc ccagccagcg ggattttgcg cagcaaagat aaagagacaa ggttcggtgg
     1561 caggacttct atcgtgcggg caatgatatt ttctgctccc agcggaggat tttccactcc
     1621 ccgcagattg cccccaccga caacccgaaa gtgcttctgc cagcggttgg gaatcgttcc
     1681 tttgctccgc aggagcggca ggtcaaaaac ctgttccggt tcggcgccgt tcagccgaaa
     1741 ttccttctgc ggcacccggg caccgatgta gtgcgagcca aagagtcgaa aaaggagtgt
     1801 ccagttgggc accacgttcc aggcgatgcg gaccaaacca gaagtgcggt tgaaggcagg
     1861 atctgaaatg aatctgcctt ttgctccgct ctcattctga aactgtaccc gcagtgcgcc
     1921 ccagcgtttc ggccagtgtc cggagaatag gtatgctgcg tacggcacgg tagtcgtggc
     1981 ccaagatgat tggtcgaaaa ggtaacggga gtcataggcg cgctcaaagc cggaaatcag
     2041 acggtgttcc accacgggcg gataacccca gcgattgcga atttgcttgg agagttcaaa
     2101 ggcgaattgc cggcggccgt cgtaatctct ggcggagacc gaccagaagg cgagtcgccc
     2161 caatgcctcg aaaggatggg tcgctttcac ggcaaaatgc ccttttatcc ttcgcgtagc
     2221 cgagacccct tccgcactca actggcgcgt gaaacctaag taaccgccgg tgacgtggag
     2281 gcccagttgt acggcatcgc gggaattgaa ccagatttgc ggacgcgcgg tcaaaaggta
     2341 ggcatcaacc ggctgagggt tgtacttcag gttatcgagt tgcaactcaa ctttgggaat
     2401 gggatagcgg ttgtttaatg ggttgacgtc ggccagccgt ccggaggggt caatctcaat
     2461 tcttttcggt ttagccggga gatcgaccgt ggcggcatac ttctttcgaa tttcacgcac
     2521 ctgcggccag atcggcagaa tctgagcgaa agtcggtttc gcagattcgt cgagaggtat
     2581 caagaaatca cgggcttggc cgtttttgag agtcacgcgt aaatccaggg gcattattga
     2641 ctcgcctttg cgcgataagc gcagttgcgt acgatagacc ttgccgctgt cggtttcgac
     2701 ccactttcct ttcggcctgg cgacagcgta gtcgatggtc tttgtcgtat ggatccactc
     2761 gtcgaaaaac cagtcgagtt cccggcccga aacctcctcc atcaccgtct gaaaatcgtc
     2821 ctcgtac
//
LOCUS       DBNGZA010000009         1679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1591962, whole genome shotgun sequence.
ACCESSION   DBNGZA010000009 DBNGZA010000000
VERSION     DBNGZA010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1591962"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..579)
                     /gene="lptC"
                     /locus_tag="AC16FU_00135"
     CDS             complement(25..579)
                     /gene="lptC"
                     /locus_tag="AC16FU_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR2992822.1"
                     /translation="MKRFVVVPIVLFVLLLACSRQEEPKPSAVLTGVPDQVLENSVVT
                     LTDKGVLSTYLLAKKVARYEAPDITKVWEIKADFFDHEGKHFSVLVADSGLVREKTRR
                     LTAWGHVVVTYDRGDRFESDSLFWDGEKEHVETDAFVKIFRKDGNMQGYGLETDSEFK
                     RFKIKRQVKGTYTEMPADSVLEKK"
     gene            complement(635..1561)
                     /locus_tag="AC16FU_00140"
     CDS             complement(635..1561)
                     /locus_tag="AC16FU_00140"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR2992823.1"
                     /translation="MEAEAVAGLGRKLNGQFVRAIELLLTCKGRVIVTGMGKSGIISR
                     KIAGMLNSTGTPAIFLHPAEGAHGDLGFVQRGDVVIAISKSGGTDEVYQLFPVFKRLG
                     VPVITITGNPKSPMAEKSDVVLDVSVAEEACPNDLVPTSSSTAALVMGDALAVVLLAE
                     RHFSPEEFALLHPGGSLGKKLLLKVADLMHTGDQVPKVSLNTPVKEAILEMTSKRFGA
                     TSVVNGKGELVGVFTDGDLRRLVEKTGDIYTVTMEKAMTKSPKTIDPEALAAAALNKM
                     ELHKITQLLVVDGARQPIGIVHLHDLLQAGVV"
BASE COUNT          325 a          514 c          462 g          378 t
ORIGIN      
        1 acagaatatc gtcacgcata ttacctactt tttctctaat accgaatccg ccggcatttc
       61 ggtgtaggtg cccttcacct ggcgtttgat tttgaagcgt ttgaactcgg agtcggtctc
      121 caggccgtag ccctgcatat tgccgtcttt gcggaaaatc ttcacaaagg cgtcggtttc
      181 cacgtgctct ttttcgccgt cccagaagag actgtccgac tcgaaccggt cgccccggtc
      241 gtaggtcact accacgtggc cccaggcggt gaggcggcgc gtcttttccc gcaccaggcc
      301 ggagtcggcc accagaaccg aaaagtgctt gccctcgtgg tcaaagaagt cggccttgat
      361 ttcccagact ttggtgatgt ccggggcttc gtagcgggcc acttttttgg ccagaaggta
      421 ggtagagagc acccctttgt cggtcaaggt gacgaccgag ttctccaaaa cctgatccgg
      481 tacgccggtc agtacagcag agggtttggg ctcctcctgc cgggaacagg cgagaagaag
      541 tacgaagagt acgatcggga ccactacgaa acgtttcatt tcgtccgcac ttccgtcaga
      601 aagattcttt tcgacttcat tcccttaaga tacgttacac tacccccgcc tgcaggaggt
      661 cgtgcaggtg gacgatgccg atcggctggc gcgccccatc aaccacgaga agctgggtga
      721 ttttgtgcag ttccatctta ttcaaggccg ccgccgccaa tgcctccggg tcaatcgtct
      781 tggggctttt ggtcatcgcc ttttccatag tgaccgtata gatgtcgccg gtcttctcca
      841 ctaagcgccg gaggtcgccg tcggtgaaga cgccgaccaa ttctcctttc ccgttaacta
      901 cggaggtagc cccgaagcgc ttggaggtca tttccagaat tgcctctttt accggggtgt
      961 tcaaagacac cttcggcacc tggtcgccgg tatgcatcag gtcggccact ttcaagagca
     1021 acttttttcc cagactcccg ccggggtgca gaagagcaaa ctcttcgggc gaaaaatggc
     1081 gctccgccag gagcaccacc gccagggcat cgcccatcac caaagccgca gttgaggaag
     1141 aggtcggcac caggtcgttg gggcaggctt cctccgctac cgaaacgtcc agcaccacgt
     1201 cgcttttctc cgccatgggc gacttagggt tgccggttat ggtaatgacc ggcacgccca
     1261 gacgtttgaa gaccgggaac agttggtaga cttcgtcggt tccgcccgat ttggagatgg
     1321 caatcaccac gtcgccccgc tggacgaagc ctaaatcgcc gtgggcccct tcggccgggt
     1381 ggaggaagat ggccggagtg ccggtcgaat tgagcatgcc ggcgatcttg cggctgatga
     1441 tacccgactt ccccatgccg gtgacaatta cccgcccctt gcaggtcagc aaaagctcaa
     1501 tcgccctgac aaactggcca ttgagtttcc ggcccagccc ggcgaccgct tccgcttcca
     1561 tagcgatcac ctgccgggca tgccgcagaa tttcggactc gccattacgc tttttgcgcc
     1621 ccgccacggg cggtgctggc cgttctaccc tttcggcaac atccgctgtt cgcatatgt
//
LOCUS       DBNGZA010000010         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1804191, whole genome shotgun sequence.
ACCESSION   DBNGZA010000010 DBNGZA010000000
VERSION     DBNGZA010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1804191"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="AC16FU_00145"
     CDS             <1..209
                     /locus_tag="AC16FU_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992824.1"
                     /translation="AAHDAPDSVRVSALDTVRHYMRSRLHVTFLFALRCPVISQPELR
                     PYLELLRVDALVNLVHCQQSDRKQ"
     gene            206..1933
                     /locus_tag="AC16FU_00150"
     CDS             206..1933
                     /locus_tag="AC16FU_00150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2992825.1"
                     /translation="MNSLRFRLILGFSFIAIVPLAVAIYFLSQRVEKTVAELAAERLA
                     AALGTLQSQLRFDGERVAGKLQILGKDPLLKRLYLLRPPGSAGTRDLSDYLAEKRFLL
                     GLDFLQVVDTGGLVTSDGSLSSATLEKTYRDSLHARARAGITSSGLVIDTVDTISVLT
                     LTATAPVRYENRSVGVVRGGLVFDAASLARLKQTSGVDLLLRDSSGRTIAATIFPAAE
                     AVSPGFEGGGRVTLAGRSYLAQTIPLETSGPPLAVITGLVSTGPADQTIASLQVTSTL
                     LGLFGLGIAIFLGIVWSRQISGPVERIAQFSQKIAQGQWDEPLTLESVRELEMLVAAL
                     NRMRDDLRTYRDKLVTSERQAAWSVMARKVAHEVKNPLTPIAISIADLQRSFEQKRPD
                     FPQILAQSAKTVTEEIETLKRLLQEFSDFAKFPPPRFAPCLLSELFTDVKTLYARDVD
                     EGRLTFTEEVREITLSADAGQIKQALINLIKNGLEAVDGQGQVRVAVNVTDSALEIDV
                     ADNGPGLTAEQKANLFTPYFTTTAQGSGLGLTIVQRIASDHGGQITVDSLPGRGTTFR
                     LLLPLELKR"
     gene            1936..3285
                     /locus_tag="AC16FU_00155"
     CDS             1936..3285
                     /locus_tag="AC16FU_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2992826.1"
                     /translation="MPSILVVDDEKNILSSLRGALGREGYQVDCAATLAEARVKLREA
                     YDFILLDVWFPEGSGLDLLEEITAGAPETVVVMMSGHGTIETAVKATKLGAYDFLEKP
                     ISLDRLLVLLRNAATTLTLRALNRRLQAERPWAVAIVGRSPAVQKLLREIEQAGPTAA
                     RVLIQGEHGTGKELVARALHAASGRREMPFVAVNCSAIPEELIESELFGHERGAFTGA
                     TQARRGQFEEAHGGTLFLDEVGDLSPRAQTKLLRVLQEGEINRVGGNRAIKIDVRVIA
                     ATNRDLAQSVKAGQFREDLYFRLAVIPLRVPPLRERTEDIPLLVEYFALEVAKETGQQ
                     AKKFAKETLNLFRRYAFPGNVRELRNLVERLFIMTQGASVTAEQVRAVLPELDSAASS
                     VARLSDAVRKFERKQIEAALAAEGGNMTKAALRLGLERSHLYKKLKKLGWQPEELHQ"
BASE COUNT          810 a         1146 c         1182 g          778 t
ORIGIN      
        1 aggccgccca cgacgctccc gatagcgtca gagtgtcggc gctggatacg gttcggcact
       61 acatgcgcag tcggttgcac gttacctttc tctttgccct acgctgcccg gtcatcagtc
      121 agccggaact ccgtccctat ctggagttat taagggttga cgctctcgtg aacttggttc
      181 actgtcagca atctgatcgg aaacaatgaa ctccctccgc ttccgcttga ttctgggttt
      241 ctccttcatt gccatcgtcc ctttggcggt ggcgatctac tttttgtccc agcgggtgga
      301 aaaaacggtg gccgagctgg cggctgagcg tttggcggcg gccctgggga cgttgcagag
      361 ccagttgcgt ttcgacggtg aacgagttgc cggaaaatta cagattctgg gaaaagaccc
      421 gctcttgaaa cggctatacc ttttgcggcc tcccggcagc gccggtacgc gggatctgtc
      481 cgactatctt gcggaaaagc gtttcctact ggggctggat ttcctgcagg tagttgacac
      541 cggcggcctg gtgacgagcg acggatcgct ctcctcagcc acgctggaaa aaacctaccg
      601 cgattcgttg cacgcgcggg cgcgggcggg gatcacctcc tccggtctcg tcatcgatac
      661 ggtcgatacc atctccgtct tgactttgac cgccaccgcg cctgtgcggt acgagaacag
      721 gagcgtcggc gtcgtgcgcg gcgggctggt ctttgacgcg gcatccctgg cgcggttgaa
      781 gcagaccagc ggcgtcgatt tactgctgcg ggacagttcc ggccgcacca ttgcagcaac
      841 cattttcccg gccgccgagg ccgtctcgcc cggctttgag ggaggggggc gcgtcaccct
      901 cgcggggcgt tcctatctgg ctcaaaccat tcccctggaa acttccgggc cgccactggc
      961 cgtcattacc ggcctggttt cgaccgggcc ggcggatcag accatcgcca gtctgcaggt
     1021 aacgtcgacc ctcctgggac ttttcggact ggggatcgcc attttcctgg ggattgtctg
     1081 gtcgcggcaa atatccgggc ccgtggagcg gatagcacag ttttcccaga agattgccca
     1141 gggacaatgg gatgagccgc tgacgctgga gagcgtccgt gaattagaga tgctggtcgc
     1201 cgccttaaac cggatgcggg acgacctgcg cacctaccgc gacaagctgg tgacgagcga
     1261 acgccaggcc gcctggagtg tgatggcccg caaggtggcc cacgaagtga aaaacccttt
     1321 gacgccgatt gccatatcga tagccgatct ccagcgcagt tttgaacaaa agcgccccga
     1381 ttttcctcaa attctggcgc agtcggctaa aaccgtgacg gaagagatag aaacgctcaa
     1441 gcgcctgctg caggagttct ccgacttcgc caagtttccc ccgccccgtt tcgccccctg
     1501 cctcctctcc gagcttttta ccgatgtcaa gacgctctat gcccgggacg tggacgaggg
     1561 gcggctgact tttaccgaag aggtgcgaga gattacgttg agcgcggacg ccggacagat
     1621 caagcaggcg ctcatcaact taatcaaaaa tggcttagaa gcggtggacg gccagggcca
     1681 ggtacgagtt gcggtgaacg taaccgactc agcgctcgaa atcgacgtgg ccgacaacgg
     1741 cccgggtttg accgcagagc aaaaagccaa cctcttcacc ccgtacttta ccaccaccgc
     1801 gcaggggagc ggcctgggcc tgaccattgt ccaaaggatc gcaagcgacc atgggggaca
     1861 gatcaccgtc gattctctac cggggcgcgg caccaccttc cgcctgcttt tgcctctgga
     1921 gttgaaaagg tagcgatgcc ttcaatctta gtcgttgacg acgaaaaaaa tattctctcc
     1981 agcctgcggg gggccctggg acgcgagggg tatcaggtgg actgtgcggc gaccctggcc
     2041 gaggcccggg taaaactccg ggaagcctac gacttcattc tgctcgatgt ctggtttccc
     2101 gagggcagcg gcctcgatct gttggaagaa attaccgccg gtgcgccgga gacggtggtc
     2161 gtcatgatgt ccggacacgg gaccattgaa acggcggtga aagccaccaa attaggggct
     2221 tacgattttc tggagaagcc gatttcgctc gaccgattac tcgtcctctt acggaacgca
     2281 gccacgacgc tcaccctgcg ggccctaaat cgccgtctgc aagcggaacg cccctgggcg
     2341 gtggcaatcg tcggccgctc cccggcggtg cagaaacttt tgcgcgagat cgagcaggcc
     2401 ggtcccactg ccgcccgggt tctaatccaa ggtgagcatg ggacaggcaa agaattagtg
     2461 gcccgggcgc tccacgccgc cagtgggcgc cgggaaatgc ctttcgtggc ggtgaactgt
     2521 tcggccattc cggaagaact gatcgaatcg gagttattcg ggcatgaaag aggggcgttt
     2581 accggagcca cgcaggcccg gcgcggccag ttcgaggaag cccacggcgg tacgctattt
     2641 ctcgatgaag tcggtgacct gagtccgcgc gcccagacca aactgctgcg ggtgttacag
     2701 gaaggcgaaa tcaaccgggt cggcggcaac cgggccatca aaatcgacgt gcgcgtgatt
     2761 gcggcgacca accgcgacct ggcgcaatcc gtcaaagcgg gccagtttcg ggaagacctg
     2821 tacttccggc tggccgtcat tcccctaagg gttcccccgc tgcgcgagcg gactgaagac
     2881 attcctcttc tggtagagta ctttgctctt gaggtggcaa aagaaacagg acagcaggcc
     2941 aagaagttcg caaaagagac actgaacctc ttccggcggt acgctttccc cggcaacgtg
     3001 cgggaattgc gcaatctggt cgagcgctta ttcattatga ctcaaggcgc cagcgttacc
     3061 gccgagcagg ttcgggctgt tctgccggaa ctggacagcg ccgcttcctc agtcgctcgc
     3121 ttgagtgacg cggtacggaa attcgaaaga aagcaaatcg aagccgccct ggccgctgag
     3181 ggcggcaata tgaccaaagc cgctctccgc ctgggactgg aacggagtca cctctacaaa
     3241 aaactcaaaa aactgggctg gcagcccgaa gagctgcacc agtgaatatg tccccgaggg
     3301 tagggctgaa ccggtgaggt cggcaaacct cattgctcag cagggtcaca accacgatgg
     3361 aaacgatgat agacaaatcg ctgtactttt cgtcccgtcc gtggtcctgg gtgtgcccac
     3421 gcccggctgt ctctgaaggc gaggatggga aggttaaggg agagaagaag aacaccaacc
     3481 gtcaggttct caccgcataa catttactga cccgtggagc ggaagctgtt gcccaccacg
     3541 ttgcgtcgtc tcttgacatc ttcccaccac ctctctacac ttcccgcatg agcagcgggt
     3601 tctttctttg ggcgtacttc tcttgcagat cgctccccaa tcagtagacc ggtcaagtac
     3661 tccatccaag cccggcgcgg gcaactcgcg tctttgcgtc tggcttcacg cgcagggaaa
     3721 caggccaaac cgggtcaacg gtcagccggc gatcgggctg ggtgccgtcg ggaaggggct
     3781 ctggactgga caaaaaattt gggggggccg tgaaataata cttgataaaa ggacaaatca
     3841 gtctttatta tagtctacca gagcagttgg gaagcgtata gataggtaaa ggagaggaag
     3901 gccctagtgg cacgca
//
LOCUS       DBNGZA010000011         4066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2440517, whole genome shotgun sequence.
ACCESSION   DBNGZA010000011 DBNGZA010000000
VERSION     DBNGZA010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2440517"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16FU_00160"
     CDS             <1..602
                     /locus_tag="AC16FU_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR2992827.1"
                     /translation="KWLVVTSAHGGPRHLVALEEACEKVSRRYGAKMICLTAGMAVDF
                     LTGKFLAEIERHRGEKFTDEERHWLRHDYHAGWWETSMMLLLRPELVDAAYTQLPPIL
                     VKLTRARSRYIPTLGKGLGYFGVPGQASAQFAQLTSQIFREKGVALLLRWIGGENVAA
                     EVHSIFYRLLIFRTAFRRWVAAFLILAAGIGLALYFAAR"
     gene            complement(615..1124)
                     /locus_tag="AC16FU_00165"
     CDS             complement(615..1124)
                     /locus_tag="AC16FU_00165"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR2992828.1"
                     /translation="MKKETEKKSSHKDPRSQEKTQKIPSGTPQRRKHVRLRIESPMTF
                     CPVPETGTIPGAAVGETKINGVILNLSRGGVLFATRTPPREHGYIVMQFRLNDGDTLT
                     DVVGKVKRVEREGDEEYFCGVEFITAGELEEMNKHTGSNNFSSAMATFDERLKDFLQQ
                     KLQSRSPVS"
     gene            complement(1162..1920)
                     /locus_tag="AC16FU_00170"
     CDS             complement(1162..1920)
                     /locus_tag="AC16FU_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HIR2992829.1"
                     /translation="MFTEKDWVAFRKGKKPELREKLLTTYLPLVKNVAGRMAMGFPRS
                     VELSDLVSTGVIGLIEAMSHFDPKRGVKFETYAVPRIRGAILDELRALDWVPRSTRAK
                     SREIERAVSKLENQLGRTPSERELAKSLRMSPDDLFLAIGDVSGTTLLSLDELLYREE
                     DNRQVPRVETLEDTNGTTVLGEIERQELKAFLVHAVTKLTEQEKLVIALYYYEELTLK
                     EIGEVMHISESRVSQIHTKAVGKLRGLIRDKFAL"
     gene            2430..3935
                     /locus_tag="AC16FU_00175"
     CDS             2430..3935
                     /locus_tag="AC16FU_00175"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR2992830.1"
                     /translation="MRDRKLVYVTLASGLFLCTFGCSKKGTQPPPVVVPKKTAKLEAK
                     KIAGTADPAGVSNPTSAFWNSDYIEFTATPDSGSTGKTYDTSPASGCQYNLANQAPGF
                     APLVRMKAAYNDSFLYLWAQWSDTTFDRNFRRLYFDGRDSLFTGGIPAFPVDITQRDS
                     LSYSPVPDSTEWTLQLNDDKIAVMWDITDAAGNGGANSPGGRFAARGCAITCHGAGKM
                     YPDSGRTDLWVWNTSRGNPFGYVTDQYTDSIFLPAGIKDDDQEKFENENWLVTARCPR
                     PRYIFDATKAGLVPYDERYYLSATDAVAAPQLSDIEIGNTYYYRRNSFDGRSCGAFDC
                     HRSDGRGDPLRTIPDLSVIGRTLSDSLIREGLGLGSSIQIHRNYTNGIAPGVPDSTLA
                     KRLTARIRAYTGAPGYYFTPPAQPAGLPAVLNVNKNAVYDAANRRYTVIIKRPLRTYG
                     ATTLQDVQFVTTREFPFSVALMDSDGKNHAGSPLQVLKFLPMVTAELFASH"
BASE COUNT          788 a         1260 c         1171 g          847 t
ORIGIN      
        1 tcaaatggct cgtggtcacg agcgcccatg gcggcccccg gcatctggtg gctctggaag
       61 aggcctgcga gaaagtttcc cgcaggtacg gggccaagat gatctgcctc accgccggta
      121 tggcggtcga ctttttaacc ggaaaattct tggcggaaat cgaacggcat cgcggcgaaa
      181 agtttacgga cgaagagcgg cactggctcc gccacgacta tcacgctggc tggtgggaga
      241 cttcgatgat gcttttgctc cggcccgaac tggtggacgc cgcctacacc caactccccc
      301 cgattctggt gaaactgacc cgggcccgct cccggtatat ccccactctg gggaaaggtc
      361 taggctactt cggagtgccc ggccaagcat cggcccaatt cgcccaactg acgagtcaga
      421 tttttcggga gaagggcgtg gcgctcctcc tccgctggat aggaggagaa aacgtggcgg
      481 ccgaggtgca ctcgatcttc taccgtcttt tgatcttccg taccgctttc cgccgctggg
      541 tcgcggcttt tctgatactg gcggccggaa tcggcctggc cctctacttc gccgcccggt
      601 aagagcggtt tttttcagga caccggggag cgggactgga gtttttgctg caggaagtct
      661 ttcaaccgct cgtcaaaagt ggccatggcc gaagaaaagt tgttggaccc ggtgtgcttg
      721 ttcatttctt ctaattctcc ggcggtgatg aactccaccc cgcagaagta ttcctcgtcc
      781 ccctcgcgtt cgacccgctt cactttccca accacgtcgg tcagggtgtc gccgtcgttc
      841 aaccggaact gcatgacgat gtagccgtgt tcccgcggcg gggtgcgggt ggcaaataag
      901 actccgcccc gggaaaggtt caagatgact ccgttgattt ttgtttctcc caccgccgct
      961 cccggaatgg tccccgtttc gggcaccggg cagaaggtca tgggcgattc aatgcgcagc
     1021 cggacgtgct tgcgccgctg cggcgtgccg gatggaattt tctgggtttt ctcctgactg
     1081 cgcgggtctt tgtggctcga tttcttttcg gtttcttttt tcattctccc tccctatcct
     1141 ccgtgaatgg aaccgaccgc tttagagcgc gaacttgtcc cggatcaggc cgcgcagctt
     1201 ccccaccgct ttggtatgga tctgcgacac ccgcgactcg gagatgtgca tgacctcgcc
     1261 gatttctttc agggtgagtt cttcgtagta gtagagcgcg atcaccagtt tctcctgctc
     1321 ggtcagcttg gtgaccgcat gcaccagaaa ggccttcagt tcctgccgtt cgatttcgcc
     1381 caggacggtg gtgccgttcg tatcttccaa cgtttccacc cggggcactt ggcggttgtc
     1441 ctcctcccgg tagaggagtt catccaaaga gaggagcgtg gtgcccgaaa cgtcgcctat
     1501 ggccaaaaac agatcgtccg gcgacatccg cagcgacttg gccagttcgc gttccgaggg
     1561 ggtgcggccc agctggttct ccagcttgga gaccgcccgc tcaatctccc gcgacttggc
     1621 gcgggtggaa cgcggcaccc agtccagcgc ccgtagttcg tccaggatgg cgccccggat
     1681 gcgcggcacc gcgtaggtct cgaacttcac cccgcgcttc ggatcgaagt gcgacatcgc
     1741 ttcgatgagc ccgatgacgc cggtggagac cagatcggag agctccaccg accgggggaa
     1801 gcccatcgcc atccgtccgg cgacgttctt caccagaggc aggtaggtcg tcaaaagctt
     1861 ttcgcggagt tccggttttt tccccttgcg gaaggcgacc caatcttttt cagtgaacat
     1921 cccggcccgt ccgttgctgg tagtggctgg agccaaagac gagcgaaaaa ctgattgggt
     1981 ggcacccgtc gtcgctcagg ggaaatgtca ggtgaggcat cggcgggcgg ttttgcaagc
     2041 cccgtgccgt ttgtgcattg gctaaccctt ttgctaaggg actgtttgcg aaagggttag
     2101 atgatcaaaa gggctggtca aaagttgccc aaagagaggc aggggaactt ttttccagaa
     2161 aaaggcggcg gaaatttcct aaaagtatca agcgggcttc cgctcttttg gcgtctcaac
     2221 ggcacatttt ctgcgaagta atctgggcgg aatctggata aaaccaggac ctttccgagc
     2281 accccagcgg ccgcagacgc ctggttcctc agaggtcact gacgaaaaag gagctccaaa
     2341 taagagttgg cgaacaaaaa aagagagtgt atctttgagc tgttggccgg tgcggagcga
     2401 aagagcgtac cggcagtttg gaggttgata tgcgggatcg aaaattagta tacgtgacgc
     2461 ttgccagcgg acttttcctt tgcacttttg gctgcagcaa aaagggcacg cagccgccgc
     2521 cggtggtggt gccgaaaaaa acggccaaac tggaagcgaa aaaaatagcc ggaacggccg
     2581 acccggccgg cgtgagcaac cccacttctg cgttctggaa cagcgattac atcgaattta
     2641 cggcgacgcc cgacagcggc tccactggaa aaacctacga cacctccccc gccagcggct
     2701 gccagtacaa cctggccaat caagctcccg gctttgcccc tttggtgcgg atgaaagccg
     2761 cctacaacga ttcgttcctc tacctctggg cgcagtggag cgacaccacg tttgaccgca
     2821 atttccggcg gctctacttc gacggccgcg attcgctttt caccggcggc atacccgcct
     2881 ttccggtcga catcacccag cgcgactcgc tctcctactc cccggtgccc gactcgaccg
     2941 aatggaccct ccagttgaac gacgacaaaa tcgccgtcat gtgggatatc accgatgcgg
     3001 cggggaatgg cggcgccaat agcccgggcg ggcgttttgc cgcgcgcggc tgtgccatca
     3061 cctgtcacgg tgccggcaaa atgtaccccg acagcggccg caccgacctc tgggtctgga
     3121 atacctcccg gggcaatccg tttggctacg ttaccgacca gtacaccgac agcatattcc
     3181 tgccggccgg aatcaaggac gacgatcagg agaagtttga aaatgagaat tggctggtga
     3241 ccgcccgctg cccccgtccc cgctacatct tcgacgccac caaagcgggg ctggtgccgt
     3301 acgacgagcg ctactactta tcggcgaccg acgccgtagc cgccccccag ctctccgaca
     3361 ttgaaatcgg caacacttac tactaccggc gtaattcttt tgacggccgc tcgtgcggcg
     3421 cctttgactg ccaccgttcc gacggccgcg gcgatcccct ccggacaatt ccggacctgt
     3481 cggtgattgg gcggacgctg tcggattcct taatccggga gggcttggga ctgggcagtt
     3541 ccattcaaat ccaccggaac tacaccaatg gtatcgcacc gggcgtaccg gactccactc
     3601 tggcgaagag actgaccgcc cgcatccgtg cctacaccgg cgcaccgggc tattacttca
     3661 cccccccggc gcagccggcc ggcctgcctg cggtcttgaa cgtgaacaaa aacgcggtct
     3721 acgacgctgc caaccgccgc tacaccgtga tcatcaagcg cccgctgcgg acctacggcg
     3781 ccactacttt acaggacgtg cagtttgtta ctaccaggga gttccccttc agcgtggcgc
     3841 ttatggattc cgacggcaaa aaccacgccg gctcgcccct gcaggtgtta aagtttctgc
     3901 caatggtcac cgccgaactt ttcgccagcc actaacgcga aataatcaac cgcgcatagc
     3961 cgccctgttc cgcccgggtt ccctgcaccg gtcggggcga aaatggacgg gatgacctgt
     4021 cagcccaatc cgccttaggc ggatgggcga cgccagggtc ttcgcg
//
LOCUS       DBNGZA010000012         1341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_213674, whole genome shotgun sequence.
ACCESSION   DBNGZA010000012 DBNGZA010000000
VERSION     DBNGZA010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_213674"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..864
                     /locus_tag="AC16FU_00180"
     CDS             181..864
                     /locus_tag="AC16FU_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR2992831.1"
                     /translation="MNDFAGLLAEAPAGALEQQLRQFEESTGVEVAVATVATTGGLAV
                     QDYANELFKRWGVGKKGKDNGVLLLVARDDRNVWIEVGYGLEPVLPDALVGQIFLNEI
                     RPRFRAGDFSGGIVAGVNAPQAAIEGKYTGQPSKKRPRSVVAYPILLVMLFIGVLVLS
                     SFVSGGRRGGKRSGWIFLPGGFWGWGGWPRGRGGYWGGGSFGGGGFGGGFGGFGGGAS
                     GGGGAGGSW"
     gene            938..1258
                     /locus_tag="AC16FU_00185"
     CDS             938..1258
                     /locus_tag="AC16FU_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR2992832.1"
                     /translation="MGVLRKSFLAALGVVSLTRKKAEEVVDQLIKEGELQRSQRTQAI
                     EELLEKAEARIKETGKRVEQKTRETVTRMRGKLDLNFDNLQSRISRLEEKVDELIEEL
                     RKRV"
BASE COUNT          299 a          317 c          449 g          276 t
ORIGIN      
        1 tgcaaagcct gaacaagtag ttccactttc ccacacaggc cgtttccgac cggcgtctca
       61 tccgcctata ttgggggcat ggtccggccc gggctgctcg cgtttctttt tttctgcctt
      121 ctttcctctt tcttttctgc ggttagctgg gccgaggaat atcccgggcc agtcggctat
      181 gtgaacgatt tcgccgggct tttggccgaa gctcctgctg gggctctgga acagcaactc
      241 cgccagttcg aagaaagtac cggcgtggag gtcgccgtag ccaccgtggc gaccaccggc
      301 gggctggccg tgcaagacta cgccaacgaa ctgttcaaaa gatggggggt cggaaaaaag
      361 gggaaagaca acggtgtcct cctcctggtg gcgagagacg accggaatgt ctggatagaa
      421 gtgggttacg gcctggagcc ggtgctgccg gatgccttag tcggtcaaat ttttctcaac
      481 gaaatccgac cgcgcttccg ggcgggggat ttttcgggag gcatcgtcgc gggagtcaac
      541 gccccccagg cggcgattga ggggaagtat acagggcagc cgtcaaagaa acgcccacgg
      601 tcggttgttg catatccaat tcttctcgtc atgctcttca ttggagttct ggtgttgtcg
      661 tcttttgtat cgggaggacg cagaggtggg aaacgttctg gctggatttt tcttcccgga
      721 ggattctggg gctggggcgg ttggccgcgt ggccgaggtg gatattgggg cggcggttcc
      781 ttcgggggcg gcggctttgg cggaggattc ggcggattcg gcggcggtgc ttccgggggt
      841 ggcggcgccg gcggaagctg gtgaaaaatc ctgaagtcca ccacggagtc acggaggaca
      901 cggagaagta cgtggttaaa taaaaaaagg agagactatg ggtgtgctga gaaagtcgtt
      961 cctggcggct ttaggcgtag tttccctgac gcggaaaaag gctgaggagg tcgtcgacca
     1021 gctcatcaaa gagggagagt tgcagcgcag ccaaaggaca caggcaattg aagaacttct
     1081 cgaaaaggcg gaagcgcgga tcaaggaaac cggcaagcgg gtagaacaga aaacgaggga
     1141 gaccgttacc cggatgcgcg ggaaactgga tctgaatttt gataatctcc aatcacgcat
     1201 cagtcggtta gaagagaaag tcgacgaatt aatcgaggag ttgcggaaga gagtctaaga
     1261 atttcaccac ggagacacag aggacacaga gaaaaacatc gcctggtcaa tgatagaccc
     1321 gtcggtgagg agagcggcgg g
//
LOCUS       DBNGZA010000013         2556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_320140, whole genome shotgun sequence.
ACCESSION   DBNGZA010000013 DBNGZA010000000
VERSION     DBNGZA010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_320140"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1087
                     /locus_tag="AC16FU_00190"
     CDS             272..1087
                     /locus_tag="AC16FU_00190"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2992833.1"
                     /translation="MPSPYATYRTPWSERITPMVQWLLIANTVIFLLQNAVGFRWMNY
                     RFGLVPLLIRENLMYWQFVTYLFLHGDLLHFLFNMFVLWMFGCEIERAWGSRQFLRYY
                     FLTGIGAGIFTFIFSFNSPIPTIGASGALFGIMVAYALLFPDRLIYVYFLFPVRAKYL
                     VLFFAGLEILASFNHTADGIGHFAHLGGMVVGYLYLKADWRWARFASAVKSWPGRRRL
                     AKIRRQMDKEARILEQVDAVLDRINQVGMENLTKEEKRLLEEASHLLHKTRST"
     gene            1110..2468
                     /locus_tag="AC16FU_00195"
     CDS             1110..2468
                     /locus_tag="AC16FU_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2992834.1"
                     /translation="MAKRILVAEGSSAIRSVAETVLRPEGYEVFTAEDGPLALRLLKA
                     LLPDLLVADWELPGFDGLALYEQAREQFPQLTKRTLFLVRHPQLVPRDVTRYGVGILT
                     KPFSPRDLLEAIDQLFRAAPTAAAPAPGGSGTTRTEAEPEQPDTDKLPPNRPAEPERG
                     AFAPPAHRVALHPPIPEPEEEEVSLEALLAEGGVELEVAGSKKTAQASDPNAAESEDP
                     TSKVAVVSPPGEPKARPLREPSREPAVSKKTPAQGWSMAGAVDLPDDLPKTSPPAYGA
                     PGDDAEIPHDYDWFLAEMERSAKGEKPRPGGVKPHDDAPTDRAYLKRPAESVTSKTVR
                     PEELKLKVEEVGTSRLGYERIVAEYRKELQKQAGEQRPGYGTAPPKAEGVKPVSTSTA
                     PRTLAPPEGTALLPGDFTDKLITEIGRQVAKEIMGRLDEDNLRALVKKQLANLSAALQ
                     KK"
BASE COUNT          498 a          789 c          750 g          519 t
ORIGIN      
        1 tgcggatcgg cataaaaacc tcctcgatgg tctcagaggc tgtcgcccgg gctgcgcacg
       61 ccagccgggc cgcggttgag gacgtggctg tgaactatct cctgcttatc aagcattccc
      121 cctgccgggt cattcctcct tatcctccgg caaggcaagt tggccgaact ttctaccata
      181 attacccgat tcggcgccgc tgtcaagacc cattctcttc tgtcaccacc attgtttctc
      241 cttcagcagc cgatatatta aatggatagg tatgccttcc ccgtacgcca catatcggac
      301 gccctggagc gagcggatca cgccgatggt ccaatggctt ttgatcgcca ataccgtcat
      361 tttcctcctc cagaacgccg tcggcttccg ctggatgaac taccgctttg gtctcgtgcc
      421 gcttttgatt cgcgaaaacc tgatgtactg gcagttcgtc acctacctct ttttgcacgg
      481 cgacctcctg cacttcctgt tcaacatgtt cgttctctgg atgttcggtt gtgagatcga
      541 acgggcctgg gggtcgcgcc agttcctgcg ctactacttt ctcaccggca tcggggccgg
      601 cattttcacc ttcatttttt ccttcaattc gccgattccc accatagggg cctccggcgc
      661 tctttttggg atcatggtcg cctatgccct cttgttcccg gaccggctca tctacgttta
      721 cttcctcttc ccggtgcggg ccaagtatct ggtgctgttc tttgcgggac tggagattct
      781 ggcctccttc aaccacaccg ccgacggcat cgggcacttt gcccatctgg gcgggatggt
      841 ggtgggctac ctctacctga aggccgactg gcgctgggcc aggttcgcca gtgccgtaaa
      901 aagttggccg gggcgcagaa gattagccaa aatccgccga caaatggata aggaagccag
      961 gatactggaa caggttgacg ctgtgctgga tcgcatcaac caggtgggaa tggagaacct
     1021 gaccaaagag gagaagcgcc ttctggaaga ggcgagtcac ctcttgcaca agacgcgcag
     1081 tacctgatgt tgacgaaacg gagattgccg tggccaagcg cattttagtc gccgagggct
     1141 ccagcgccat ccgcagcgta gccgagaccg tcctgcggcc cgaagggtat gaagtgttca
     1201 ccgccgaaga cgggcctctg gcattgcggc tgctcaaagc actcctgccc gacctgttgg
     1261 tcgccgattg ggagcttccc ggtttcgatg gtcttgccct gtacgagcag gcgcgggagc
     1321 agtttcccca gctgaccaag cgtaccctct ttctggtgcg gcatccacaa ctggtgccgc
     1381 gagatgtgac tcgctacgga gtcggtattc tcaccaagcc gttcagcccc cgcgaccttc
     1441 tggaagccat tgaccaactg ttccgtgccg cacccacggc agctgctccc gccccgggcg
     1501 ggtcgggtac gactcggacc gaggctgaac cggagcaacc ggataccgat aaactgcctc
     1561 cgaaccgtcc ggctgagccg gaacgcggag ctttcgctcc gccggcgcac agggtcgcgc
     1621 tccacccacc gattcccgag ccggaggaag aggaggtttc cctcgaggcg ctcttagccg
     1681 agggaggggt ggaactggaa gtggccgggt cgaagaaaac agcgcaggcc agtgatccca
     1741 atgcagcgga aagtgaagat cctacgagca aggtggccgt cgtgtctcct ccgggagaac
     1801 ccaaagcccg ccccctgcgg gagccgagcc gggaaccggc ggtctccaag aagactccgg
     1861 cacaggggtg gtctatggcc ggagccgttg atctgccgga cgatctgccc aagaccagcc
     1921 cgccggccta cggggcgccg ggcgacgatg cggagattcc ccacgactac gactggtttt
     1981 tggccgagat ggaacggagc gccaagggtg agaagccgcg tcccggcgga gtgaaaccgc
     2041 acgatgacgc ccccaccgat cgggcgtatc tgaaaaggcc ggctgagtcg gtcacctcga
     2101 agacggttcg gccggaagaa ttgaagctga aagtggaaga ggtgggcacc agccgcttgg
     2161 ggtacgagcg gatcgttgcc gaataccgca aggaactcca gaagcaggcc ggggagcagc
     2221 gtcccggcta cggcaccgcg ccccccaaag cggaaggggt aaagccggtc tccacttcga
     2281 ccgctccgcg caccttggcg cccccggaag gaacagcctt gctccccggt gatttcaccg
     2341 acaagctcat taccgaaatc ggccgccagg tggcgaaaga aattatgggc cggttggacg
     2401 aagacaatct gcgcgctttg gtcaaaaaac aactggccaa cctgagcgcg gcgcttcaga
     2461 aaaagtagcc cccgcagttc ttccggaccg taaaatctcc tcttttcgtc gttttccttt
     2521 tcgtattgtt gataccggtt gcagcgttgt atgttc
//
LOCUS       DBNGZA010000014         1623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2233, whole genome shotgun sequence.
ACCESSION   DBNGZA010000014 DBNGZA010000000
VERSION     DBNGZA010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2233"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC16FU_00200"
     CDS             complement(<1..183)
                     /locus_tag="AC16FU_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992835.1"
                     /translation="MSTKLLALLVATSLFGSPVAGQQLRIDSTANLLPPVVRFLNPTE
                     HTLIVQWRADTIGIGQV"
     gene            complement(192..773)
                     /locus_tag="AC16FU_00205"
     CDS             complement(192..773)
                     /locus_tag="AC16FU_00205"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2992836.1"
                     /translation="MRFTSGAVGGVSILKIRLLILLLVGAVLPGCGQKKASDERLTTA
                     PYREYLSLAPIVEIHGETADSTQLGDDRYLEDNLDYLLAKHEWVLVNFSAYWCKDCRK
                     FDPDYQAVAGLPKYKGIFFTKAEIDGTKGNENFRTRFALPGIPVTILFHNGQIVEEGG
                     QKAILFGQRGDKTKADLTALLDHFYQPAAKGPA"
     gene            complement(749..>1623)
                     /locus_tag="AC16FU_00210"
     CDS             complement(749..>1623)
                     /locus_tag="AC16FU_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR2992837.1"
                     /translation="INVNKPVHLKSALRDSATISGGSPYAVTLSSGCVLDGFIVRSEA
                     VGNILVQSAAGIRPKIVRNRIYSAPVGIQVSNGSNSAIIDHNLISRTVTAISLANTTG
                     SSKITHNTIVANDNGIVVNLSPNGDIVNNIVDSCGNGLVVTGGCPAHSYNDFSNISGS
                     TYAGCVAGVGEIACDPGFCDRSIEDYTLFDNSCAIGAGPGGDTLGAFPVACVACAIAT
                     FGDVTGDATADILDIVAVIGYVVFGSPALVPGAFAGDVNCDGVVDITDIVALINNVVF
                     SAALCNPCDSPLAP"
BASE COUNT          354 a          472 c          461 g          336 t
ORIGIN      
        1 gacctgacca atcccaatcg tgtccgcacg ccactggact atcagcgtat gttccgtggg
       61 attgagaaag cggacgacgg gcggcagaag gttagcggtg gaatcaatac gtaattgctg
      121 tcccgcaacg ggggagccaa agagagaggt ggcgacgagc aaagccaaaa gtttcgtgga
      181 catacggacg cttacgccgg acctttggcg gcgggctggt agaagtggtc caaaagcgcc
      241 gtcaaatcgg ctttggtctt gtccccccgc tggccgaaaa gaattgcttt ctgtccgccc
      301 tcttctacga tctgaccgtt gtggaagaga atcgttaccg gtattccggg cagagcaaag
      361 cgcgtccgga agttttcgtt ccctttcgtc ccgtctattt cggcttttgt gaagaaaatc
      421 cccttgtact tcgggagccc ggctaccgcc tggtaatcgg gatcaaactt gcggcagtcc
      481 ttgcaccagt aggcggaaaa attcaccagc acccactcgt gcttggccaa aaggtagtcg
      541 agattgtcct ccaggtagcg gtcatccccc agttgggtgg agtccgccgt ctcgccgtgg
      601 atttcgacaa tcggggccag cgacaggtac tcccgatagg gcgcggtggt cagccgctcg
      661 tcggaggcct tcttctggcc acagccgggg agaactgccc cgaccaacag aagaatgaga
      721 agacgaatct tcaggatgga tactcctcct acggcgccag aggtgaatcg catgggttgc
      781 acaatgcggc gctgaaaacc acgttgttga tcaaggcgac gatgtccgtg atgtccacca
      841 cgccgtcgca gttaacgtca ccggcaaagg ctccggggac caaagccgga cttccgaaca
      901 ccacgtagcc gatgaccgct acgatgtcga gaatatctgc ggttgcatca cccgtaacgt
      961 cgccgaacgt ggcaatcgca caggctacgc aggccaccgg aaaggcccct aacgtatccc
     1021 cgcccggccc cgcgccgatg gcacaagagt tgtcgaaaag ggtgtaatct tcaatgctac
     1081 ggtcgcagaa gcccggatcg caggcgatct caccgacgcc tgcaacacaa ccggcgtatg
     1141 tgctccccga aatgttcgag aagtcgttgt acgagtgggc cgggcagccc ccggtaacta
     1201 ccagtccgtt tccgcacgag tcaacgatgt tgttgacgat gtccccattc ggtgacaggt
     1261 tcacgacaat cccgttgtca ttggccacga tggtattgtg cgttatctta gaagaaccag
     1321 tcgtgttggc cagagagatg gcggtgacgg ttctgctgat cagattgtgg tctatgattg
     1381 cactattgct cccgttggat acctgaatcc ccaccggcgc actgtaaatc ctgttccgca
     1441 caattttcgg tcgaataccg gcggcacttt gcaccaggat attccccacg gcttcactgc
     1501 ggactatgaa tccatccagc acacacccgc tggagagcgt caccgcgtag ggcgacccgc
     1561 cgctgatagt ggccgaatca cgcagcgcac tcttcaggtg caccggcttg ttcacgttga
     1621 ttc
//
LOCUS       DBNGZA010000015         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2229074, whole genome shotgun sequence.
ACCESSION   DBNGZA010000015 DBNGZA010000000
VERSION     DBNGZA010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2229074"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="AC16FU_00215"
     CDS             complement(<1..1335)
                     /locus_tag="AC16FU_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006457196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2992838.1"
                     /translation="MVRLLRFGDTLRVGSAAILLLLISFSLASGQVTLLYQKNGTAAG
                     DKLGYSAAGVGDVDGDGKSDFIVGAPFTHPGGRINAGSAYVYSGATGVLLFQIDGVSA
                     GDVLGITVAGAGDVNGDGRSDFIIGIPGRQNYTGSAYVYSGATGALIYQKDGATAGQV
                     FGLAVSGINDLDGDLRADFIVSAGYASPGGVSLAGSVFIYSGATGSLIYQKDGGAAGD
                     IFGFSVSGAGDLDGDGRGDFIVGACSADPGGRPGAGSAYIYSGATGALLYQKNGASNS
                     VELGWSVSGAGDVDGDGRDDFIIGAPSVDFGGIGSVFVYSGTTGALLAQKVAPVAGSD
                     FGITVAGTGDVDGDGKGDYAIGTSRANVSGIGNAGSVLVYSGGSGALLHQINGSAAGD
                     SLGFWVADAGDVNGDGKTDIIIGAPRTDPGGITDAGSVYVYSFSPTGVTRVEF"
     gene            2191..2391
                     /gene="rpsU"
                     /locus_tag="AC16FU_00220"
     CDS             2191..2391
                     /gene="rpsU"
                     /locus_tag="AC16FU_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR2992839.1"
                     /translation="MTGVRVRDDETFEKALRRFSKSCEKAGLMADIKKHQHFEKPSER
                     RKRKLAAARRKTRRMRYLDEAA"
BASE COUNT          583 a          738 c          623 g          472 t
ORIGIN      
        1 aaactccact ctggttacgc cggtggggga gaaggagtag acatagaccg agccggcatc
       61 ggtgattccg cccggatcag tgcggggagc accgatgata atatcggttt tcccgtctcc
      121 attcacatca cccgcatctg cgacccaaaa ccccagactg tcgccggcgg cagacccgtt
      181 tatctggtga agaagagcac cactcccgcc ggaatagacc agaactgatc ccgcgtttcc
      241 aatcccgctt acgttcgcac gcgaagtgcc gattgcgtaa tcgccctttc catctccatc
      301 cacatctcca gtgcctgcaa ccgtaatacc gaaatcactt cccgccacag gggcaacttt
      361 ctgagccagc aaggcacctg tggtaccgga gtaaacgaat actgagccga tgcctccaaa
      421 gtctacactg ggagcaccaa tgatgaaatc atccctgcca tccccatcca cgtccccggc
      481 tccactcacc gaccagccca gttcaaccga gtttgaagcg ccattttttt ggtaaagcag
      541 tgcacccgtt gcacctgagt agatgtacgc cgagccggct cccggacgac cacctgggtc
      601 ggcactgcac gccccaacga taaaatcgcc tctcccatca ccgtcgagat ctccggcacc
      661 gctcacggaa aacccaaaaa tgtcccccgc tgcaccaccg tctttttggt agatgaggct
      721 tccggttgcg ccggagtaaa tgaagaccga tcctgctaag gagactcccc ctgggctggc
      781 atagcccgcg ctgacgataa aatcagccct caagtcacca tccagatcgt taattccgga
      841 cactgcaaga ccaaagacct gcccagctgt ggcgccatct ttctggtaaa tcagtgcccc
      901 tgtggcacca gaatacacgt aggccgagcc ggtgtagttc tgcctcccgg gtatcccgat
      961 aatgaagtca gacctgccat caccattcac atccccggct ccagccaccg tgatgccgag
     1021 aacgtccccg gccgagacac cgtctatttg gaacaggaga actccggtcg ccccggagta
     1081 gacgtaggcc gagccagcgt tgatacgccc accggggtga gtgaaaggag cgcctacaat
     1141 gaaatctgat ttgccgtcac catccacatc accgaccccc gctgcagaat acccaagctt
     1201 gtcaccagcg gcagtaccgt ttttctggta gagcagcgta acttgccccg aagccaatga
     1261 aaaggaaatt agcaacagaa gaatcgccgc gctccccacc cggagcgtgt caccaaaacg
     1321 gagtaaccga accatttatc ctccccggca catctgcgcc gagaaaaccc tatctgttaa
     1381 tcaacatcac tttgtttcct tgtcaagcaa ttttcgcgcc cttgggtagt ttttccctcc
     1441 cctgcgaggg tcaaagaccc cgccagaggc gggggagggt gagggagggg tcaatgctct
     1501 ttctctcgcg taggcgggtc gcagactcgt accgagactt tagtcccgca tagatacatg
     1561 aggtactccg actcgtctct gagccggtcg gagacgcaaa tctgcttcat ctgctgtgag
     1621 atgattcttt tcccctaatc tctaatcctc gtaagagtcg catcgacccc atttctccga
     1681 atttgtgatt gtcgcggagg cacacaggtg ccccttcccg cgctaatttg gagttgcctt
     1741 gggggtaaaa cgaccccgct atccccatag gcgaggcttc cagcctcgca gaaatttggg
     1801 gtcgctccgg gggtaaaacg gacaaagtgc gatatagtag gaggactgat accaggcgac
     1861 tggcctccgg catgctgagg cctcggtaaa aaacgaagca tcacgcgcca gtgcccgcag
     1921 tcctccgcca ggagaaaaag gccacgtggc cgccctggaa aatccagggt aaaaagtcaa
     1981 accggaaccg atgcggaaag gggaaataag ttcagcatct cttccgcccg gcaaccttcc
     2041 cggctaatct tttgtgtcag aatgggatag caccttccgg cactggggag tggctctgta
     2101 ttgcttgaca aagtaaatat ccggccctta tcttgccgat agtgagaata tgaccgtaaa
     2161 ccttaaccgt gcgaagggag ggtgagatgc atgacgggag ttcgggtccg tgacgacgag
     2221 accttcgaaa aagcgctgcg ccgctttagc aagagttgtg aaaaagccgg cctgatggct
     2281 gatataaaaa agcaccagca cttcgaaaaa ccgtccgagc gccgcaaaag gaaattggcc
     2341 gcggcgcgcc gcaaaactcg gcggatgcgc tacctcgacg aggcggcatg atccgggaga
     2401 agatcgaggc cgaggt
//
LOCUS       DBNGZA010000016         1668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_850773, whole genome shotgun sequence.
ACCESSION   DBNGZA010000016 DBNGZA010000000
VERSION     DBNGZA010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_850773"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /gene="sov"
                     /locus_tag="AC16FU_00225"
                     /gene_synonym="sprA"
     CDS             <1..>1668
                     /gene="sov"
                     /locus_tag="AC16FU_00225"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR2992840.1"
                     /translation="PGTQFVGYSEQKQGLFGIKTQAKLGGLDLTMITSQDKSNSQRFN
                     FHAGAQGATIYLRDYQYLRYAYYDLGRNTKADPQRGVNEFDPARDSIIQLEVYRWKSS
                     TAPGTGSIALPGICYVDPNDPNRPDVDTLERETGYFERLKPDSNYFVNSLLRPFVVYF
                     EPQLQESDIIGVYLKIKRSNPDTTIEYGCIDLAACDSILKLKLIKRRRGGSRPTDVTY
                     SYEWKNVYNLGARNIDWDGLNVQIFKGSQGDESNPARQEHDPGLANYLQVTGIDQFNA
                     LGVRLIDNKVDKREEVLDLRRGHLFFPERYPFNSDVSYAGDTATSLRTRIAAIYDSIN
                     VPDVLQRNTQYYLEIKSLTGRQKDFSLGRVNILENSEEVLLNNRRLVRGKDYNIDYQL
                     GQISFLNEEVLNPNADLSINFDYSPFLSTEKKALFGLRGDYQLSDNFRAGTTVLFRNE
                     TSNDQKPKIGQEPNRTLLWDADASYRFQAPWLTRAVNALPLVEAAAPSTVNLSGEIAR
                     SFPNPNTRQNAYLDDFEGSKETVDLGIFREGWTAASPPVGKGPSSRGR"
BASE COUNT          394 a          502 c          446 g          326 t
ORIGIN      
        1 gcccggcacg cagttcgtcg gctattcgga acagaagcag gggttgttcg gaatcaaaac
       61 tcaggccaaa ctgggcgggc tggacctgac tatgattacg agtcaggaca aaagcaactc
      121 ccaaaggttt aacttccatg ccggtgcgca gggagccacc atttacctgc gcgattatca
      181 atacctccgc tacgcctact acgacctggg ccggaacacc aaggccgacc cgcagcgcgg
      241 ggtaaatgaa tttgatcccg ccagagatag catcatccaa ctggaagtct acaggtggaa
      301 gtcgagcact gctcccggca ccgggagtat cgcgctcccg ggtatctgtt acgttgaccc
      361 caacgacccc aaccgtcccg acgtagacac tctggagcgg gaaaccgggt acttcgagag
      421 gttgaagcct gattccaatt acttcgtcaa ttctctcctg cggccctttg tggtctattt
      481 cgaacctcag ttgcaggaat ctgacatcat cggcgtttat ctgaaaatca agcggagtaa
      541 tccggataca acgatagaat acggctgtat cgaccttgcc gcctgcgaca gcattctcaa
      601 attgaagctt attaaaaggc ggcgaggcgg gagccgtccg accgacgtca cctatagtta
      661 cgagtggaag aacgtctaca acctgggcgc gagaaacatc gactgggacg gtttgaatgt
      721 gcagatcttc aaaggcagcc agggcgatga atcgaatccc gcccggcagg aacacgatcc
      781 gggcctagcg aactaccttc aggttacggg gatcgaccag ttcaatgccc tgggggtgag
      841 gctcatagac aacaaggtgg ataagcgcga agaggttctt gacctcaggc gcgggcacct
      901 cttctttccg gagcgctatc ctttcaattc cgacgtgagc tatgcggggg acaccgccac
      961 cagtctgcgc acccgcatcg cggccattta cgattccatc aatgtccctg atgtcctcca
     1021 gcggaacacc cagtactacc tcgaaatcaa aagtctcacg ggccgtcaaa aagatttctc
     1081 cctgggccgc gtgaacatcc tggagaattc cgaagaggtc ctcctgaaca accgccggct
     1141 cgtccgcggg aaggattaca atatcgacta ccagttgggg caaatttcgt tcttgaacga
     1201 ggaggtgctg aaccccaatg ccgatttgag catcaacttc gactactccc ccttcctctc
     1261 cacggagaaa aaggccctct tcggcctgcg gggcgactac cagttaagcg acaacttccg
     1321 ggcaggcacg acggtcttgt tccgcaacga aacctccaac gaccagaaac ccaaaatcgg
     1381 ccaggagccg aaccgcaccc tgctctggga tgccgacgcc tcgtaccgtt tccaggcccc
     1441 ctggctcacc cgggcggtca atgccttgcc tctggtggaa gcggccgcgc cttctaccgt
     1501 caatctctcc ggagaaattg cccggagttt tcccaatccg aacaccagac agaatgccta
     1561 cttggacgat tttgagggga gcaaggaaac ggtcgatctg ggcattttcc gtgaagggtg
     1621 gaccgccgcc agcccgccgg tcggcaaagg tccctccagc cgcggccg
//
LOCUS       DBNGZA010000017         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1805695, whole genome shotgun sequence.
ACCESSION   DBNGZA010000017 DBNGZA010000000
VERSION     DBNGZA010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1805695"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2810
                     /locus_tag="AC16FU_00230"
     CDS             <1..2810
                     /locus_tag="AC16FU_00230"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2992841.1"
                     /translation="AAIGAKTVAEGSLLSFRVTATDSNGTTPRLFAQNLPVNATFTDS
                     LNGAGGFVFQPSFVQSGIYQVLFFASDSLAADSELVQITVTDAGNQAPRIDSLGPFTV
                     RENDSLVFVVRAVDPESGAVRLAMVKGPTGAVFTDSLNGHGLFRFRPTYLQASIDSAV
                     FTATDNAVPPLSASRRVQITIVDANQPPVLDSIGPKAVLAGQVIQFRVHAKDTTDASP
                     GRLFLSASPLPLHATFVDSANGAGSFRFAPDFTQIGFDTVSFFVFDDDLPVMSDREDV
                     VIEVRSANRAPVLDPIGTKMVREGDVLSFRVHATDPDGTIPRLYTSALPQNATFVDSG
                     NGAGSFRFTPNFVQAGLYSVTFSATDNIATAREAVLIQVTEAGNQRPILTTPDTTTLT
                     EAETVVLPVFASDPDTTIPRLSAFNLPPNATFADTGRGSGVFRFTPSYIQAGTYTIGF
                     VASDGALADTNLMTVIVRDAGNQPPVLDSIPDRTVTEGGTLTFTVHASDPDTTKPFLT
                     AAPLPANATLVDNRNFTATFSFSPSFFQAGQYQITFYARDFLTTALVDSQKVTITVTN
                     VNQFPIVDSIVSRPSSRTIAEGETLLAVVYARDPDNTIPRLTAANLPLQSNFVDSGNG
                     HGLFRYTPDFCQTGNSAQKFFSVVFTATDRDADSATATQSLQITLNNTQKAPVLNPIG
                     PLSITEGDTLRTVISSFDPDCTTPTLAVQNLPRNATFTSSSGVGLFTFAPGFNQAGTY
                     NVLFIATDNTGRADSELVAVTAVEFGNHAPQITLTAPLPTPLTRGDTTILRVGDLSSA
                     RVSATDADSTIPVLSAVPVPRNAVFVDSGNGRGVFRITPDSSQIDSTYLVRLIASDGL
                     ATDTVLLKFRIRPFFRGDCNHDGVIDLSDIVQLIQYVVNDQFPPIPPGAGDVKLDGVI
                     DLVDVVGLISFVVNGSPVPLP"
     gene            complement(2917..>3144)
                     /locus_tag="AC16FU_00235"
     CDS             complement(2917..>3144)
                     /locus_tag="AC16FU_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992842.1"
                     /translation="CGLLSSPPCVARGDVTNDGLIDIADIVALIQDVVFETGVPNGLA
                     GDADCDAVRDISDIILLIQYVVFGTPSPCCL"
BASE COUNT          597 a         1022 c          853 g          672 t
ORIGIN      
        1 tggccgccat cggtgccaaa actgtagccg aaggttcgct cctctccttc cgggtgacgg
       61 ccaccgattc caacggcacc acgcccagac tcttcgccca gaacctgccg gtgaacgcta
      121 cattcaccga tagtttgaac ggagcgggcg ggtttgtttt ccaaccgagt ttcgtgcagt
      181 caggtatcta ccaggttctc ttcttcgcat cggattctct ggcggccgac agcgaactcg
      241 ttcaaatcac ggttacggat gccggcaacc aggcgccgcg gatcgactcg ctcggtcctt
      301 ttaccgtccg tgaaaatgac agtctggttt ttgtcgtgcg ggctgttgat ccggagagcg
      361 gtgccgtgcg cctggcgatg gtcaaaggcc cgacgggtgc ggtcttcacc gacagtctga
      421 acggccatgg cctcttccgc ttccggccga cctacctgca ggcaagcatt gactcggcgg
      481 tcttcaccgc cactgataac gcggtgccgc cgctttcggc ctcgcgccgg gtgcagatta
      541 ccatagtgga tgccaatcag ccaccggttt tggattcgat tggtcccaaa gccgtcttag
      601 ccgggcaggt aatccagttc cgggtgcatg ccaaagacac gaccgatgcc agtccgggcc
      661 gtctgttcct ttctgcttcc ccgctgccgc tccacgccac ctttgtcgac tcggccaatg
      721 gcgccggatc gttccggttt gcgcccgact ttacccaaat cggtttcgac accgtttcct
      781 tctttgtctt cgatgacgac ctgccggtga tgagtgaccg ggaagatgtg gtgattgaag
      841 tgcgctcggc caaccgggcg ccggtcctcg atccgatcgg aacgaagatg gtgcgcgagg
      901 gagatgtcct atcattccgc gtgcatgcca cggacccgga cgggacgatt ccgcgtctgt
      961 acacgtcggc actgccgcag aatgccacgt tcgtcgatag cggcaatggt gccggctcgt
     1021 tccgcttcac tcccaatttc gtccaggccg ggctctactc agtcaccttc tcagccacgg
     1081 ataatattgc cacggcgcgc gaagcagtct tgatccaggt gaccgaagcc ggcaaccagc
     1141 ggcccatcct gaccaccccc gataccacca cgctcaccga ggccgagacc gtcgttctgc
     1201 cggtcttcgc cagcgatccc gataccacca ttccgcggct ttcggctttc aacctgcctc
     1261 cgaacgccac gtttgcggac accggcaggg gcagcggggt cttccgattc accccttcct
     1321 acatccaggc ggggacgtac accatcggtt ttgtggcgtc ggatggcgcg ctggcggata
     1381 cgaacttgat gacggtgatc gtccgggatg ccggcaacca gccaccggtt ctggattcaa
     1441 ttcccgatag aaccgtcacc gaaggcggga cgctcacctt taccgtgcac gccagcgatc
     1501 cggatacgac caaaccattc ctgacggcgg cgcctttgcc ggccaacgcc actttggttg
     1561 ataaccggaa cttcaccgca actttctcct tcagcccgag tttcttccag gcggggcagt
     1621 accagattac cttctacgcc cgagactttt tgacgacggc cttagtcgac agccagaagg
     1681 tgacaatcac ggtgaccaac gtgaatcagt tccccatcgt cgattcgatc gtcagccggc
     1741 cgtccagccg caccattgcc gagggagaaa cgcttctggc ggtggtctac gcacgcgacc
     1801 cggacaacac cataccgcgc ctgaccgcgg cgaacctgcc gctccaatct aatttcgtcg
     1861 attccggcaa cgggcacggc ctcttccgct acactccgga tttctgccag accggcaaca
     1921 gtgcgcaaaa gttcttctca gtcgtcttca ccgccaccga ccgggatgcc gattccgcca
     1981 ccgccaccca gtcgctccag atcaccctga acaacaccca gaaggcgccg gtactcaatc
     2041 cgatcgggcc tttgagcatc accgaggggg acacgttgcg caccgtcatc tcgtcttttg
     2101 accccgactg taccaccccc acgctggcgg tgcagaactt gccgcgcaac gcaacgttta
     2161 cgtcgagcag cggggtcggt cttttcacct tcgctccggg tttcaaccag gcgggaacgt
     2221 acaacgtgct cttcatcgcc acggacaata ccggccgggc cgacagcgaa ctggtggccg
     2281 tgacggccgt tgaattcggg aaccatgcgc cgcagattac cctgacggcg cccctcccga
     2341 ccccactcac gcggggagat acgaccatcc tgcgggtcgg cgatctctca tcggcgcgtg
     2401 tttccgccac tgacgccgac agcaccattc cggtcctttc cgcggtgccc gtgccgcgga
     2461 acgcggtctt cgtcgattcc gggaatggac ggggtgtatt tcggattact cccgattcca
     2521 gccagatcga ttcgacctac ctcgttcgtc tcatcgcttc ggacggactg gcaaccgata
     2581 cggtgctcct gaagttccgc atccggcctt tcttccgcgg cgactgtaat cacgatgggg
     2641 ttattgacct gtccgacatt gtccaactca tccaatatgt ggtcaatgat caattcccac
     2701 ccattcctcc gggggcgggg gatgtcaaac ttgacggcgt catcgacctg gtggacgttg
     2761 tcggtttaat ctcctttgtg gttaacggca gccccgtgcc gctaccgtag atcgactttt
     2821 ctcagatatg agaagacccc cctacttagt tggagggtgt ggagattttc ggaaaaatct
     2881 tcggcaattt ttaaatcaga ctctaaaagc aggcctttag aggcagcacg ggcttggcgt
     2941 tccgaatacc acgtattgga ttagcaggat aatgtccgat atgtctcgga cggcgtcaca
     3001 atctgcgtcg cccgccagcc cattaggaac gccggtttca aagactacat cctggattag
     3061 ggcgacgatg tccgcaatat cgattaagcc atcgttggtt acatctccgc gcgccacgca
     3121 gggaggagaa gatagcagac caca
//
LOCUS       DBNGZA010000018         5281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_533548, whole genome shotgun sequence.
ACCESSION   DBNGZA010000018 DBNGZA010000000
VERSION     DBNGZA010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_533548"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="AC16FU_00240"
     CDS             complement(<1..1109)
                     /locus_tag="AC16FU_00240"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR2992843.1"
                     /translation="MYLLSAEQMRAIDRRAIEDLGLPILDLMEKAGLGVALAARQMLG
                     GELEGKTIAVLCGKGNNGGDGLVAARYLAQGGGKVTIYLLARATELAGAAKTNLTRAQ
                     EAKIRIVELSDAKQLPETCDLFIDAIVGTGFQGTLTSLAAEVLQKVSSSERAVLAVDI
                     PSGVDATTGEIKGPVAKARKTVTFAWPKVGQFLFPGKFYSGEVQVVDIGIPKSGAEPE
                     KVNLHLLTKSEIAQNLPLRKPDGHKGTFGKVLVIAGSEGLTGAACLASLAALRSGCGI
                     VILGCPESLNDIFEIKLTEVMSRPLPEVKHRRVLSLRSLGEILKLMEWADVVALGPGV
                     GTHHETQDLVRRLLMRIEKPVVLDADGINALAKDNS"
     gene            complement(1113..1955)
                     /gene="pyrF"
                     /locus_tag="AC16FU_00245"
     CDS             complement(1113..1955)
                     /gene="pyrF"
                     /locus_tag="AC16FU_00245"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR2992844.1"
                     /translation="MKNTTPFREKLTRAIQNAQSFLCVGLDPEPEKLPAACQKAADPV
                     LEFNRRIIEATKDIVCAYKPNLAFYEALGKSGWETLNETCKAIPKNLPIILDAKRGDI
                     GHTARLYAQALFDQLGGDAVTVNPYLGGDALAPFADYVDRGILVLCLTSNPGAGDFQT
                     RLVNGHPLFLEVARKVVEWDRHRNFGLVVGATYPDQLQQVREAAGDLPILIPGVGAQG
                     GDLEKSVQYGTRNGTNLAIINVSRAILYASSGSDFAEKARAKALELKTAMLSAVGAKI
                     TLSY"
     gene            complement(1957..2895)
                     /locus_tag="AC16FU_00250"
     CDS             complement(1957..2895)
                     /locus_tag="AC16FU_00250"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR2992845.1"
                     /translation="MKGGKRYGKPDLSVTIAGIPFANPVWVASGTVGYGEELAEIIDL
                     SKIGAIVTKTITLEEREGHPYSRTVETAGGMLNAIGLQNVGVERFIKEKLPFLAQIKK
                     TRIVVNIGGSSIDDFVAVAQRLSDHGPIDMLEANISCPNVKEGGHEFSATPQPAFQVI
                     AAVVKASRFPVIAKLSPNTANVAAVARAVAEAGAAAISLINTLVGLAIDIDARRPVLS
                     NVTGGLSGPAIKPVALAMTWQVARAVKLPIIGMGGIMTVEDTVQFFLAGATAIQIGTA
                     NFVDPTTSLTIVQGLEKYLRQKKISRITDLVGKLET"
     gene            complement(2892..3125)
                     /locus_tag="AC16FU_00255"
     CDS             complement(2892..3125)
                     /locus_tag="AC16FU_00255"
                     /inference="COORDINATES: protein motif:HMM:NF021903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR2992846.1"
                     /translation="MLQATCRIANERQIFGQIAVENHMACSFGICMGCMVRCRKKVTP
                     PSEETDRTEISREVYQRACTEGPIFTMHEVVWP"
     gene            complement(3094..3744)
                     /locus_tag="AC16FU_00260"
     CDS             complement(3094..3744)
                     /locus_tag="AC16FU_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR2992847.1"
                     /translation="MKAKLTGEVTAVRFFSPAIVRLDFQAPHITAHCRPGQFIALKAS
                     DRYDPLLRRPMSLFTVDPRKKTAAILFKIFGPGTRYLANRKKGQTLDFLGPLGNRFVI
                     PARTDRVIIVAGGLGLAPLNFLLEELIRKRKHPQDRLLLLLGARVKEDLVALRKSLRL
                     PTKTALATDDGSVGFRGTVTQLLEKELDGKKKRSPPHRPFRLRPRGDAPGHLPDCQ"
     gene            complement(3839..>5281)
                     /locus_tag="AC16FU_00265"
     CDS             complement(3839..>5281)
                     /locus_tag="AC16FU_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2992848.1"
                     /translation="FMKMDNHREAAAAFEKVLKYSPPPEQYFNARFNQAGCLCQVKEL
                     NQGIAILRGLAKDERFYKNSGRVNLKLAEAHLLLDSVSTAIDLCEKVTSLFPQTPDAA
                     QAYYQLGEIYQKRLGDLKKAKEQFDKAASEGRGSEYGRLAVARSSDIAKLETYQKAIS
                     SEETEKAAQSQFLLGEVYYHQLDQPDSAVREYRSVVENHPESPYAAKALAALAYIYLE
                     EHQDTTQARAAYRALLDNYPHADCARDAIYFLGLAGTAADTGYPAKVFADGERLLAND
                     HNVDSALTLFRSIPVRFPQSQYVPRAFFTVAWILENFKAPADSSVYYAYRLVADSFPN
                     SPYAQQALVKLGSPPRRTAPPPPPVVKPDTTVIPKDTLALDTTLVTRAAIPMAPRPIH
                     DPQFVYPPSQKDSGFQDRLRFKILINFLGNVADVELLNPSSIQEIDEAAKLAVHNLQF
                     DPLKINPRQYNTWFIYELDVIPPAQAPFHP"
BASE COUNT         1075 a         1446 c         1572 g         1188 t
ORIGIN      
        1 gagttatctt tggcgagcgc attaatccca tcggcatcca gcacaaccgg tttttcaata
       61 cgcatcagga gccgccggac caggtcttgc gtctcatggt gcgttcccac cccggggccc
      121 agggcaacga catccgccca ttccattaac ttcaaaattt cgcctaaact ccgcagggag
      181 agaacgcgtc tgtgcttcac ttcaggcagg ggccggctca tcacttcggt cagtttgatt
      241 tcgaaaatgt cgttcaaact ttcaggacaa cctaaaatga ctatcccgca gccgctgcgg
      301 agcgcggcta aagaggccag gcaggcggcg ccggtgagac cttccgagcc cgcgatgact
      361 aatactttgc cgaaagtgcc tttgtggccg tcgggctttc ggagggggag gttttgtgcg
      421 atttctgatt tggtcagaag atgcaggttg accttttccg gctcagcacc gctcttcggg
      481 atgccaatgt ctaccacttg cacctcgccc gaatagaatt tccccggaaa gagaaactgc
      541 cccacctttg gccacgcaaa agtcacggtc ttccgcgctt ttgccactgg ccctttgatt
      601 tcgccggtgg tcgcgtccac acccgaaggg atatcgactg ccagcacggc ccgctcgctt
      661 gaggacactt tctgcaagac ctccgccgcc agactggtga gggttccctg aaaacccgtg
      721 ccgacgatgg catcgatgaa caggtcgcaa gtttcaggca gttgttttgc gtccgatagt
      781 tcaacgatcc gaatcttcgc ttcctgagcg cgagtcaaat tcgttttcgc ggcaccggct
      841 aattcagttg ctctggctaa gagataaatc gttactttcc cacccccctg ggccaaataa
      901 cgtgctgcta ctagaccatc gccgccatta ttccctttgc cgcagaggac ggcaattgtt
      961 ttgccttcca gttcgccacc caacatctgc cgcgctgcca gcgccacgcc cagaccggct
     1021 ttttccatca aatcgagaat cggaaggccg aggtcttcaa tcgcccggcg gtcgatggcc
     1081 cgcatctgtt cagcactcaa aaggtacatc ggttagtaac tcagagtaat ctttgcgccc
     1141 acggcggaaa gcatggccgt tttcaattcc agtgccttcg cgcgcgcttt ttcggcgaag
     1201 tcgcttccgc tggatgcgta cagaatggct cgcgagacat tgatgatggc cagattggtt
     1261 ccgttcctcg ttccatactg caccgacttt tccagatcgc cgccctgagc gcccacgccg
     1321 ggaatcaaga tgggcaagtc tccggccgcc tcgcggacct gttgcaattg atcgggatag
     1381 gtggcgccga cgacgagacc gaaattcctg tgtctatccc actctaccac cttccgggca
     1441 acctccagga agagcggatg accgtttacc aaccgcgtct gaaaatcacc ggcgcccgga
     1501 ttagaggtca aacagagcac caaaatccct ctatccacat agtcggcaaa aggagctaga
     1561 gcgtctcctc ccagataggg attcaccgtc acggcatcgc cgcctaactg gtcaaaaagc
     1621 gcctgcgcat agagccgggc ggtatgcccg atatcgccgc gcttggcatc cagaataatg
     1681 ggcaggtttt taggaattgc cttacaggtc tcgttcagcg tttcccagcc gcttttcccc
     1741 agagcctcgt agaaggcgag gttcggtttg taggcgcaga cgatgtcttt ggtcgcttcg
     1801 ataatccggc gattgaattc cagtaccggg tcagccgcct tttggcaggc cgccggcagt
     1861 ttttccggct ccggatcaag tcccacacag aggaatgatt gtgcgttctg gatggccctg
     1921 gtgagttttt cccgaaatgg agtggtgttc ttcaccctac gtttccaatt tgccgaccag
     1981 gtcggtgatg cgggaaattt tcttctggcg cagatacttt tccagaccct ggacgatggt
     2041 gagactggtg gtgggatcga cgaaattggc agtgccgatc tgaatggcgg tggctccggc
     2101 caggaagaac tgcaccgtat cttccaccgt cataatcccc cccatgccga tgatggggag
     2161 tttgaccgcc cgtgccacct gccaggtcat cgccagggcc accggtttaa tagccgggcc
     2221 ggagaggccg ccggtgacgt tcgaaaggac tggtcgccgg gcgtcgatgt caattgccag
     2281 acccacgagg gtgttgatga gtgagatcgc ggcggcgccc gcctccgcca cggcgcgggc
     2341 gacggcagcg acgttggcgg tgttgggcga gagtttggca atcaccggaa agcggctggc
     2401 tttgacgaca gcggcaatga cctggaaagc gggctggggg gtggccgaaa attcgtgtcc
     2461 gccttctttc acattcgggc aggaaatgtt ggcttccagc atgtcgatcg ggccgtggtc
     2521 ggaaagacgc tgggccactg ccacgaagtc atcaatgctg gagccgccga tgttcaccac
     2581 aattcgggtt ttcttaattt gcgccaggaa gggtagtttt tctttgatga aacgctccac
     2641 gccgacattc tgcaggccga tggcattcag catcccgccc gcggtttcca ccgtgcgcga
     2701 gtagggatgg ccttcccgtt cttccagggt gatggttttg gtgacaatgg ccccgatttt
     2761 agagaggtcg attatttcag ccagttcctc accgtagccc accgtgccgg aggcgaccca
     2821 gaccggattg gcgaaaggaa tcccggcaat cgtcacggag agatccggtt ttccgtatct
     2881 tttccccccc ttcacggcca gacgacctca tgcatggtga agatcgggcc ttcggtgcag
     2941 gcacgctggt agacttcgcg ggaaatttca gttcggtccg tttcctccga cggaggagtc
     3001 acctttttgc ggcagcgcac catgcacccc atacagatgc cgaaactgca ggccatatgg
     3061 ttctccacgg caatctggcc gaaaatctgc ctctcattgg caatccggca ggtggcctgg
     3121 agcatcccct cggggccgca ggcgaaaagg acggtgtggc ggtgatcgtt ttttttttcc
     3181 gtcaagttct ttttccaaaa gctgggtgac cgttccccgg aatccgacgg agccgtcatc
     3241 ggtggccagg gcggtcttcg tcggcagccg caacgacttc cgcagcgcta ccaggtcttc
     3301 ttttacccgg gcgccgagga gcagcaaaag acgatcctgc ggatgctttc gcttccggat
     3361 cagctcttct aacaggaagt tcagcggtgc caggccgagg ccgccagcga caatgatgac
     3421 ccggtcggtg cgggcgggga tgacgaagcg attccccaga gggcctaaaa agtcgagcgt
     3481 ctgtcctttt tttcgattgg ccaggtagcg cgttccgggg ccgaaaattt tgaacagaat
     3541 ggcagccgtt tttttgcggg ggtcgacggt gaaaagcgac atcgggcggc gcaggagcgg
     3601 atcgtagcga tcactggctt tgagagcgat aaactggccg gggcggcaat gagccgtaat
     3661 atgcggggct tggaagtcaa gacgtacgat cgcgggagag aaaaaacgga cggcggtgac
     3721 ctccccggtc agtttagctt tcatcagata attcattata acattttttc cgcagaagaa
     3781 aaagccggag aaaaccaagc atttactgag acgatccgag agacggaaaa gatgtttact
     3841 aggggtggaa cggagcctgt gctggtggaa tgacgtccag ttcgtagatg aaccaggtgt
     3901 tgtactgtct tggattaatc ttcagggggt caaattgaag attatgcacc gccagtttgg
     3961 cggcttcatc gatctcttga atgcttgaag gattcaggag ttccacatcg gctacgttac
     4021 ccagaaaatt gatcaatatt ttgaagcgaa gtctgtcttg gaagccggaa tccttttggg
     4081 aagggggata gacgaactgc ggatcatgaa tgggccgcgg ggccatgggg atggcggccc
     4141 gggtgaccag agtagtatcg agggcgagcg tatctttggg aatgacggtg gtatccggtt
     4201 tgactaccgg tggcggcgga ggagccgtgc ggcggggagg actccccagt ttgaccaagg
     4261 cctgttgggc gtagggagaa ttcgggaagc tgtcggctac gaggcggtag gcgtagtaga
     4321 cgctcgagtc cgcgggggct ttgaaatttt ccagtatcca ggcgacggtg aagaaggccc
     4381 gcggcacgta ctgggactgg gggaaacgca cggggatact ccgaaaaagg gtgagcgccg
     4441 aatcgacgtt gtggtcgttg gctaagagcc gctcaccgtc ggcaaatact ttcgcaggat
     4501 aaccggtgtc cgccgccgta ccggccaatc ccaaaaagta gatggcatcc cgggcgcagt
     4561 cggcgtgagg gtagttatcc aggagtgccc ggtaggcggc ccgcgcctgg gttgtgtcct
     4621 gatgctcctc caggtaaatg taggccagcg cggccaacgc tttggcggcg taggggcttt
     4681 ccgggtggtt ttcgaccaca ctccggtact cccgcaccgc cgaatcgggc tgatccaact
     4741 ggtggtagta gacttcgccg agcaagaact gcgattgagc ggccttttcc gtttcttcgg
     4801 aggagatcgc tttttggtag gtttcgagtt tggcgatgtc gctggagcgc gcgaccgcca
     4861 gccggccgta ttccgagccg cgaccttcgc tggcggcttt gtcgaactgt tctttggctt
     4921 ttttcaaatc gccgagccgt ttttgataaa tctcgcctaa ctggtagtag gcctgggccg
     4981 cgtcgggagt ttgcgggaag agggaagtta ctttttcaca caagtcgatg gccgtggaaa
     5041 ccgaatccaa aaggaggtgg gcttcggcca gtttcaggtt cacgcgacct gaatttttgt
     5101 agaagcgctc gtctttggcc agaccccgca ggatcgcgat accctggttc aattctttga
     5161 cctggcagag gcaaccggcc tggttaaagc gggcattgaa gtactgctcc ggcggcggac
     5221 tgtatttcag aactttctca aaagcggcgg cggcttcccg gtgattgtcc attttcataa
     5281 a
//
LOCUS       DBNGZA010000019         1329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2654583, whole genome shotgun sequence.
ACCESSION   DBNGZA010000019 DBNGZA010000000
VERSION     DBNGZA010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2654583"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="AC16FU_00270"
     CDS             complement(<1..1030)
                     /locus_tag="AC16FU_00270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2992849.1"
                     /translation="MSQRMAAMAQPEVSVVVPMHNESANLPVTLARIAQALRLSGKSW
                     EIIAVNDGSTDDTERKIQAFAREELNVRLVSYQPNHGRGYALRQGFAAARGEIICTTD
                     ADLSYGEEYLPAMVKVLQEHPEVDVVIGSPYMPGGKVEKVPKFREMISRAANKILGFA
                     LPGNLATVTGILRAYRRPSLEALGLESDGKEIHLEILSKAVAMGFGVAEMPATLWGRQ
                     KGKSKFKFRATSISHLLFSFFEKPMLLFGLVGLLLFSLAVLGGDYVIYLWQTGQLNPN
                     RPLMTLIVILSLTGLQVLLFGFLGTQIVHLRREIYRLQRTTRVMAQSLGRQPAEPSVP
                     GTDTKKIYA"
BASE COUNT          278 a          399 c          387 g          265 t
ORIGIN      
        1 ccgcatagat tttcttggta tcagtcccgg ggacggatgg ttccgccggc tggcggccca
       61 gagactgggc cattacccgg gtggtgcgct gcaaccggta gatttcccgg cgcaggtgga
      121 cgatttgcgt tccgagaaaa ccgaacaaga gcacctgtaa tccggtcagc gaaagaatca
      181 cgatcaaggt catgagcggc cgattgggat tcaactgccc ggtctgccag aggtagatga
      241 cgtagtcgcc gccgagcacc gcaagggaaa agaggagcaa accgaccagc ccgaagagca
      301 gcatcggttt ctcgaagaac gaaaacaaca ggtgcgatat ggaagtggcg cggaatttga
      361 atttcgactt gcccttctga cgcccccaga gggtggccgg catctccgcc acgccgaacc
      421 ccatggcgac agctttcgag agaatctcca ggtgaatttc ttttccgtcc gactccagac
      481 cgagcgcttc cagagaggga cggcggtagg cgcgcaggat gccggtgacg gtagccagat
      541 tccccggcag ggcaaaacct aaaatcttat ttgcggcgcg gctgatcatt tccctgaatt
      601 tggggacttt ttccactttt ccccccggca tatagggtga accgatgacg acatcgactt
      661 ccgggtgctc ctgcaatacc ttgaccatag ccggcaggta ctcctcgccg taactcaaat
      721 cggcgtcagt ggtgcagatg atttcccccc gcgcggccgc aaatccctgc ctcagcgcat
      781 acccccggcc gtggttgggc tggtaactca ccagacggac gtttaactct tcccgggcga
      841 aggcttggat cttccgttcg gtatcgtcgg tggagccatc gttgacggca ataatttccc
      901 acgacttacc ggaaagacgc agggcctggg caatccgcgc cagggtgacc ggcaggttgg
      961 ccgactcgtt gtgcatcggc acaacgaccg aaacctccgg ctgggccata gccgccatcc
     1021 tctgtgacat actcatcctc tctctggcat cggcggctga cgggtgttcc ttgagcgtta
     1081 ggggtcttcg gctcggcctt gcgcttgacc cctccctcac cctcccccgc ctccggcggg
     1141 gtcttcgacc ctcaagggga gggaagcctt tcggcagcga caaaggaatg acggagttac
     1201 cgataaacac aggaggtact acccaaacaa aaaccccgcc ctacgcttga tgcagggcgg
     1261 ggtttgtacg aactgcggaa aagatcatgt ctccgcgtcg ttgatggtac agctcagcgt
     1321 gccggtttc
//
LOCUS       DBNGZA010000020         4293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2018326, whole genome shotgun sequence.
ACCESSION   DBNGZA010000020 DBNGZA010000000
VERSION     DBNGZA010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2018326"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC16FU_00275"
     CDS             <1..235
                     /locus_tag="AC16FU_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992850.1"
                     /translation="QIKQALIDSGAVFSSLTGSGSAVFGIFLDQPIEQEITRRLQALP
                     EYRKPNLWRVSVTRPVIAAEPVCASPDASTGSG"
     gene            344..670
                     /gene="spoVG"
                     /locus_tag="AC16FU_00280"
     CDS             344..670
                     /gene="spoVG"
                     /locus_tag="AC16FU_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010297759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR2992851.1"
                     /translation="MEITDIRITLRDEEKLKAIVNVTFDNCFAVRGVKVISGESGYFI
                     SMPSRKRPDGTFQDIAHPTNSEMRKKMEDKILEAYERALKSREAQAAAAPAPRVESAS
                     GGAASE"
     gene            719..790
                     /locus_tag="AC16FU_00285"
     tRNA            719..790
                     /locus_tag="AC16FU_00285"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:752..754,aa:Gln,seq:ttg)
     gene            1004..1573
                     /locus_tag="AC16FU_00290"
     CDS             1004..1573
                     /locus_tag="AC16FU_00290"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR2992852.1"
                     /translation="MKRTSLNSIKVITMLLLGSALVALAGCSRGKEQQLQADLRKFTD
                     EQAMTATYLVRFDSLDFDVFSNQKWDLLNVSHADDITVNWPDGHQTKGIAKHIEDLKA
                     MFVFAPDTRIEDHPIKFGSGEWTCVTGTFAGTFTRPMPVGNGKTIPPTGKKFSLPMCT
                     VGHWKDGKMIEESLFWDNQTFMKQIGLAK"
     gene            1694..2638
                     /locus_tag="AC16FU_00295"
     CDS             1694..2638
                     /locus_tag="AC16FU_00295"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HIR2992853.1"
                     /translation="MLLLVLVAGVFGLVLAQENKEKMTNNKLTPEEERVIVHKGTERP
                     FTGKYYDFDEKGTYTCKRCGALLYRSDDKFDSHCGWPSFDDAIPGAVKQVPDADGMRT
                     EILCAKCGAHLGHVFTGEKFTAKDTRYCVNSISMNFIPAGAGSSKPEAKTQTQTQKAY
                     FAGGCFWGTEYYLKQAKGVVDVKVGYMGGHTKNPTYQQVCSGTTGHIETNEVTFDPAK
                     TSYEEVAKLFFEIHDPTQVNRQGPDIGEQYRSVVFYADDGQKQTAEKLIGILKKKGFK
                     VATELRPASAFWVAEDYHQDYYTKKGGEPYCHTRVKRF"
     gene            2808..3812
                     /locus_tag="AC16FU_00300"
     CDS             2808..3812
                     /locus_tag="AC16FU_00300"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR2992854.1"
                     /translation="MTPVLTERRPRTGEKPEVARTVSETPRIKILTGNSNRKLAERIA
                     AYTKIPLSECEVSRFSDGEIFVQINENIRGADVFIVQSTNPPAENLMDLLLMFEAARR
                     ASAKRITAVIPYFGYARADKKDQPRVAISARLVADLITVAGTNRVITMDLHASQIQGF
                     FSIPSDHLYASPVFNRYFMNLGLTNLVIVSPDVGGIRITRAFAKKFEAPLAIVDKRRP
                     APNHAEVLNIIGEVEGKTVIIRDDLVDTAGTLTQAAQALADHGAKEIYACCTHPVLSG
                     NSLELIAKSKIKKMIVADTIDTAQKKLPRQIEILSMADLFGESILRIAREESVSVLFD
                     "
     gene            3897..>4293
                     /locus_tag="AC16FU_00305"
     CDS             3897..>4293
                     /locus_tag="AC16FU_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR2992855.1"
                     /translation="MQAVTLAVKARSEKGKEIVKKMRRQGVIPGVVYGPEGEPTPVAV
                     EAKVFDNLLRSGLSENAIIDLSITNGGQSTKKVLIREVQLHPVSGQILHIDFQHITLT
                     RKITVNIPVHVVGLAVGVKDTGGILQHTLR"
BASE COUNT         1022 a         1199 c         1220 g          852 t
ORIGIN      
        1 tcaaatcaag caggcactca tcgattctgg tgcggtgttt tccagcctca ctggaagcgg
       61 ctcggcagtg ttcggcattt ttctggatca gccgatcgaa caggaaatta cccgccgctt
      121 gcaggcgttg ccggaatacc ggaaacccaa cctgtggcgg gtgagcgtca cccggccggt
      181 gatagcggcg gagccggttt gtgccagtcc agacgcgagc accggttcag gatagacagc
      241 cccgcctctg gcggggcctt acggcaggga gcatatcgag agaggaattg aggagtaatg
      301 gttcgatccg cctcaggcgg actcaccata gaaggagcag tctatggaaa tcaccgacat
      361 ccggatcacc ctgcgcgatg aggagaagtt gaaagccatc gtcaacgtca ccttcgacaa
      421 ctgctttgcg gtgcgggggg tcaaggtgat cagcggggag agcggctact tcatctcaat
      481 gccttcccgc aaacggcccg acgggacctt ccaggacatt gcgcacccga ccaactccga
      541 aatgcgtaag aaaatggagg ataaaatcct ggaggcctac gagcgggcgc tgaaatccag
      601 agaagcccag gccgccgcgg ctcctgctcc gagagtcgag tctgcttccg gcggggccgc
      661 atcggagtag ccattctctc cggcagcccc gctcttccgg tcaaagcgat attcattttg
      721 gggcgtagcc aagttggtaa ggcacaagac tttggatctt gcatcccagg ttcgaatcct
      781 ggcgccccag ataaaacaag gatcagggat tagagattag ggattagtaa attctcggcg
      841 tcagacgata tttccggttc gcctgcccga gccgggtgtg ggggctcggc cagagaatgc
      901 ggtgccacag cggttgccct ggtgccgatt gcaagggagt ctttttgact aacgccgacg
      961 cgaaagagga cgtaaatcat ccaacaagcc ataggaggtc gatatgaaaa ggacatcgct
     1021 caactcgatc aaggtgatca ccatgctttt gctgggatcg gccctggttg ctttagccgg
     1081 ctgctccaga ggcaaagagc agcaactgca ggcggatttg agaaaattca ctgacgaaca
     1141 ggcaatgacc gccacctacc tcgtccgctt cgactcgctg gattttgacg tcttcagcaa
     1201 tcaaaaatgg gacctgctga atgtcagcca tgcggatgac atcaccgtca actggcccga
     1261 cgggcaccaa accaaaggga tcgccaagca catcgaagac ctgaaggcga tgttcgtgtt
     1321 tgcgcccgat accagaatcg aagaccatcc gatcaaattc ggttccggtg aatggacctg
     1381 cgtcacgggc acattcgccg gaacgtttac cagaccgatg cctgtcggca acggcaaaac
     1441 gattccaccc accggcaaga aattcagtct gccgatgtgc accgtaggcc attggaaaga
     1501 cggcaagatg atcgaggaat ctttgttctg ggacaaccag acgttcatga agcagatcgg
     1561 gctggccaaa tagacccacc ggtcgttgcc ggtttgggat tgcggttcta aggttcagaa
     1621 aatgaggaat ttcttgaaaa aactcgggac gaacttcaga ctctccgatc ttatgggggg
     1681 cctgaaaagg attatgttac tactggtgct ggtggcagga gtttttggct tggttttggc
     1741 acaggagaat aaggagaaaa tgaccaacaa taagttgacc cccgaggaag aacgggtgat
     1801 cgtccacaag ggcacggagc ggccctttac cggcaagtac tacgatttcg acgagaaggg
     1861 gacctacacc tgcaagcggt gcggcgccct gctctaccgc tcggacgaca agttcgactc
     1921 ccactgcggc tggcccagct tcgatgacgc cattcccggc gcggtcaagc aggtgcccga
     1981 tgccgacggg atgcggacgg aaattttgtg cgccaagtgc ggcgcgcacc tgggacacgt
     2041 tttcaccggc gaaaaattta ccgccaaaga cacccgctat tgcgtcaatt cgatttcgat
     2101 gaactttatc cccgccggag cgggttcgag taagccggaa gccaaaaccc agacgcagac
     2161 gcaaaaggcc tattttgccg gcggctgttt ttggggaacg gagtactact taaagcaggc
     2221 caaaggggtc gttgacgtga aggtcggcta tatgggcgga cacacgaaga atccgaccta
     2281 ccagcaggtt tgcagcggca caaccggcca tatcgaaacc aacgaggtga ccttcgaccc
     2341 ggcgaagacc tcgtatgaag aagtagctaa actctttttc gaaatccacg acccgaccca
     2401 ggtgaaccgc cagggaccgg atatcgggga gcagtaccgg tcggtggttt tctacgcgga
     2461 cgacgggcag aaacaaaccg ccgaaaagtt aatcggcatt ctgaaaaaga agggtttcaa
     2521 agtggcgacc gagctgcgcc cggcctccgc attctgggtg gcggaagatt accatcagga
     2581 ttactacacc aaaaagggcg gggagccgta ctgccatacc cgggtcaaga gattctaaat
     2641 caggctggaa ctcaacctct aaactttaag ccccgaccga gaatcggggc ttatttttgc
     2701 ccatacaaaa tatctcgaat gtcaagtctt gactttttac ttcttatctg ccattttttc
     2761 ccttaaatgc ccttcatttt tctgttcaat cgcaactgag ggtggccatg acgccagttc
     2821 tgactgaacg cagaccgcgc actggagaga agccggaggt ggcccgcacc gtgagcgaaa
     2881 cgccacgcat taaaattctc accggcaaca gcaaccggaa gttggccgag cggattgcgg
     2941 cctataccaa gattccctta tccgagtgcg aggtctcgcg tttttccgac ggcgagatct
     3001 tcgtccagat caacgagaac atccgagggg cggacgtttt catcgtccaa tccaccaacc
     3061 cgccggcgga aaatttgatg gacctgctcc tgatgtttga ggcggcgcgg cgggcctcgg
     3121 ccaagcggat cacggcggtc atcccctact tcggctacgc ccgggccgac aagaaagacc
     3181 agccccgggt ggcgatcagc gcaaggctcg tggccgatct catcaccgtg gcgggcacca
     3241 accgggtcat cacgatggat ctccacgcct cgcagattca gggatttttc agcattccct
     3301 ccgaccacct ctacgcgtca ccggttttca accgctactt tatgaatttg ggcctgacca
     3361 atctggtaat cgtctcgccc gacgtgggcg gaatccgcat cacccgggct tttgccaaga
     3421 aattcgaggc gcctttagcc atcgtggaca agcgccgccc ggcgccgaac catgccgaag
     3481 ttttgaacat catcggcgaa gtggagggga aaaccgtcat catccgggac gacctggtcg
     3541 acaccgccgg gaccttgacc caggcggcgc aggcgctggc cgatcacggc gccaaagaaa
     3601 tttacgcctg ctgtacccat cctgtcctgt cgggcaattc tttggagtta atcgccaaat
     3661 cgaaaatcaa aaaaatgatc gtggccgaca ccatcgacac cgcccagaag aaactgccgc
     3721 gccagatcga aatcctctcc atggccgacc tgttcggcga gtcgatcctg cgcatcgccc
     3781 gggaagagtc ggtctcggtg cttttcgatt gaggttgcat ttgggccggg aaagactata
     3841 tttggcaatg ccccgctggt ttgcggggtt ctgatttcac attctcagga gcaatgatgc
     3901 aggcagtcac cttagcggtc aaggcccgca gcgaaaaggg caaggaaatc gtcaaaaaaa
     3961 tgaggcggca gggggttatc cccggcgtgg tctatgggcc ggagggtgag ccgacgccgg
     4021 tggccgttga ggccaaagtc ttcgataatc ttttgcgctc cggtttgagc gaaaacgcca
     4081 tcatcgacct ttcgattacc aatggcggtc aatccaccaa aaaggtgtta atccgcgagg
     4141 tgcagctgca cccggtctcc gggcagatcc tgcacatcga cttccagcac attacgctca
     4201 cccggaagat caccgtgaac atcccggtcc acgtcgtcgg cttagccgtg ggagtgaaag
     4261 acaccggggg cattttgcag cacaccctgc gcg
//
LOCUS       DBNGZA010000021         6844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2760767, whole genome shotgun sequence.
ACCESSION   DBNGZA010000021 DBNGZA010000000
VERSION     DBNGZA010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2760767"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="coxB"
                     /locus_tag="AC16FU_00310"
     CDS             <1..675
                     /gene="coxB"
                     /locus_tag="AC16FU_00310"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR2992856.1"
                     /translation="ATPLAREVRADFWTMLAIIAPFLLGAEGLLLYAVLKFRAKPGRK
                     PATFHENFKLEVFWTALPAVALVLIAIPAFRTMHAMNVPPKSDLVVEVIGHQFFWEYR
                     YPRYGITLAEEPLVVPAGKVVTLNCTSVDVIHSWFVPAFGVKQDANPGRITNAWFKAD
                     APGTYRGQCAELCGKLHAQMYITVKVLPEDEYKQWLAQKIAALPVPAGAPPATAQPPT
                     TGKGGA"
     gene            672..2252
                     /gene="ctaD"
                     /locus_tag="AC16FU_00315"
     CDS             672..2252
                     /gene="ctaD"
                     /locus_tag="AC16FU_00315"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR2992857.1"
                     /translation="MSNRYGSLWSRLRPWLVTTDHKKIGIMYLLVALFMAIFGGAFAG
                     LLRAQLATPAGKVLAPEFYNQFLTMHGTLMIFFVIIPAMVGFGNYLVPLQIGARDMAF
                     PRLNALSLWIVFPAATLMLGSFLVTGGTAQAGWTGYVPLTSRAYSGTPGVDMWILGLH
                     LVGLSSILGAINFLVTIINMRAPGMKYMKMPLFTWTWFVNATLMLVATPVLAGAITMV
                     LSDRILGTGFFRPSQGGDPILYQHLFWFYSHPAVYIMILPGMGLVSQIIPAFSRKLLF
                     GYRGMVGATAGIGIIGFLVWGHHMFATGIDPALRTYFSVMTMLIALPTGIKVFSWIAT
                     AWGGTIRFTTAMLFALGFVSLFVIGGMSGVILANIPVDVQVHDTYFVVAHLHYVLFGG
                     SLMALLGGLFFYFPKMTGRMLDEKLGKITFWLSYIGMNVTFFPMHWLGILGMPRRISS
                     YPDRPAFTFINQLETFGYVIILAGAILLLYNMWRSLRRGAAASADPWQVNDIQKTLDW
                     TVSSPPPVENFKEIPVVT"
     gene            2249..3358
                     /locus_tag="AC16FU_00320"
     CDS             2249..3358
                     /locus_tag="AC16FU_00320"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR2992858.1"
                     /translation="MSAEVVLTQPAAPSAGAEGEKIALARARIASWLVVLALVVFVLI
                     LWGGIVRLSGSGLSIPEWPIVNGSLLPPFSSGDWDAVYRTYHREILHITDLGSPEAIP
                     PRRFKAMFTIEYLHRFLASLTGILFLAIFVRAWRLKPVRSQVKGLLITAAFFLVAQAV
                     LGGIVVKRELEAEVVAGHLGLAFLFFATILWMALRLLRLPAAARVLPQSKLRLLAWSA
                     TGTVFLQILSGGLVAGTHAGWMMNTFPKMGDFLVPPLSVLWSSIYPTRWHNLVENQVL
                     LQFFHRWWAFVATLVVLFLIVRAIPFPLSPRGRLGLRAVGSVVVLQVLLGIGNLLMRV
                     PFWLSLVHLATALFLFGLLVLITHELRYRYETFPR"
     gene            3339..4214
                     /locus_tag="AC16FU_00325"
     CDS             3339..4214
                     /locus_tag="AC16FU_00325"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR2992859.1"
                     /translation="MKHSLANYLKLTKPEILLLVVLTGATALVMEGSLLAHPLRFGLV
                     ILALILTGGCANALNQYFERDLDSRMLRTRRKRPLPLGEISPRRALVFALALGAAALL
                     ILGMVFNLFSAVLALMTILFYSLFYTLWLKPRSHLNIVIGGAAGAMGPVIAWAAATGS
                     LSLTPWILFLIIFFWTPPHFWALALCVKEDYAKAKIPMLPVIKGDEETRRQIVVYTLW
                     LVGISLALLLVKAGAFSITLALALGGFFLWKAVQTFRRRTHRAAWGLFGYSILYLWVL
                     FIGMMADAVWHRAPH"
     gene            4233..4766
                     /locus_tag="AC16FU_00330"
     CDS             4233..4766
                     /locus_tag="AC16FU_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR2992860.1"
                     /translation="MNKKLKKNVATGLIFAGVAVGMFAFAFANIPVFRLYCQKVGIAL
                     SPNAKVKPGPAVSSNREVQILFTGVVAGGAPIFFSAKEAFMTVRLGKETETAYRFVNL
                     SADTIRFRPVHSILPEAAARRFSLKKCFCFDDQTLLPKQEVVLPVIFSINPDLDPEVE
                     QVTLNYTLFRKPLHGGN"
     gene            4766..4933
                     /locus_tag="AC16FU_00335"
     CDS             4766..4933
                     /locus_tag="AC16FU_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992861.1"
                     /translation="MSERKLEHSPWPGADVTGRLKRRNRWLGVFFLLFVLGIIALSLV
                     IFSKHGFPPHW"
     gene            4949..5755
                     /locus_tag="AC16FU_00340"
     CDS             4949..5755
                     /locus_tag="AC16FU_00340"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR2992862.1"
                     /translation="MADIAHTTTAVRHLAHAEHPEHGSPWPIILAGGLGLLAAGVLNL
                     IWQTKYGFPLLVTGGTIALLAVSNWAHTLIREKNFLENLFVDETWMKNGMKLFLISEA
                     AIFGAFFAHHYYARVHSPAWPPVGSPHLETRLPAIATLVLMASSGTCQWAHNLLRKGK
                     RVASQRGILLTMVLGILFLGAQGYEWGFLKAYDDFTLKNGAFGTSFFIMTGFHGLNVS
                     IGIILLSIVYVRLRLGHFTPERHFSMIAASWYWHFVDIVWIFLFGTMYLI"
     gene            complement(5853..5926)
                     /locus_tag="AC16FU_00345"
     tRNA            complement(5853..5926)
                     /locus_tag="AC16FU_00345"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5890..5892),aa:Met,seq:cat)
     gene            complement(5990..6766)
                     /locus_tag="AC16FU_00350"
     CDS             complement(5990..6766)
                     /locus_tag="AC16FU_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2992863.1"
                     /translation="MKLGSLDISYRCLRVLQRHRDVFWKNAKSNLIPPFFDPLFYLLA
                     MGYGLGALVAKIKGLSYPEFIAPAIAASVALTAPSFECIVGTLVRMKEQKTWDAMIHT
                     PVSVEDVITGEILFGAARSVIQAVSILIVIWLFGLIHSPLVVLLPLVIFISGIIHGSL
                     AMTYTSFFPEFNYMDYYFTLVMTPMFLFAGTFFPVERLPGWAHTVAWFMPLYHTTTIS
                     RALTTGLGSWHLALNVLWLLVACGLFYLLALVAMKRRVIK"
BASE COUNT         1252 a         2066 c         1943 g         1583 t
ORIGIN      
        1 gccacgcctt tggcccgcga ggtgcgggcg gacttctgga cgatgctcgc catcatcgcc
       61 cctttccttt tgggtgccga gggacttctg ctctacgccg ttttgaaatt ccgcgctaag
      121 cccggacgga aaccggccac ctttcacgag aatttcaaac tggaagtctt ctggaccgcc
      181 ttgccggcgg tggcgctggt tttgatcgcc atccccgcct tccgcaccat gcatgccatg
      241 aacgtccctc ccaaaagcga cttggtagtg gaggtgatcg gccaccagtt cttctgggag
      301 taccgctacc cgcgttacgg catcacgctg gcggaggaac cgctcgtggt tccggccgga
      361 aaggtggtca ccttgaactg taccagcgtg gatgtgattc actcctggtt tgtcccggcc
      421 tttggtgtca agcaggatgc caacccgggc cgcatcacca atgcctggtt caaggccgat
      481 gcgcccggca cctaccgcgg ccagtgcgcc gagttgtgcg gcaagctcca cgcccagatg
      541 tacatcaccg tcaaggtcct gccggaagat gaatacaagc agtggctggc ccaaaaaatt
      601 gcggctctcc ccgtccctgc cggagcaccc cccgcaacgg ctcaaccccc aactaccggc
      661 aagggaggag catgagtaac cggtacggca gtctctggtc gcgcttgcgg ccctggctcg
      721 tcaccactga ccacaagaag atcgggatca tgtacctcct cgtggcgctc tttatggcga
      781 ttttcggcgg cgccttcgcc ggacttctgc gggcgcagct ggccaccccg gcgggcaaag
      841 tgctcgcccc ggagttctac aatcagtttt tgaccatgca cggaaccttg atgatcttct
      901 tcgtcatcat ccccgccatg gtcggcttcg gcaactatct ggtgcctttg caaatcggcg
      961 cccgcgacat ggccttcccg agattaaatg cgctttccct ctggattgtc ttccccgccg
     1021 ccactttaat gctcggcagt tttttggtga ccggcggcac cgcccaggcc ggctggaccg
     1081 gctacgtccc tttaacctcc cgcgcctact ccggcacgcc gggcgtcgat atgtggattc
     1141 tgggactgca cttggtcggg ctcagttcga tcctcggcgc gatcaacttt ttggtgacca
     1201 tcatcaacat gcgcgcgccc gggatgaagt atatgaaaat gccccttttt acctggacct
     1261 ggttcgtcaa tgccaccctg atgctcgtcg cgacgccggt cttagccggc gccattacga
     1321 tggtactcag tgatcgcatc ctgggaaccg gtttcttccg ccccagccag ggaggtgacc
     1381 ccatcctcta ccagcacctc ttctggttct actcccatcc ggcggtttat atcatgatcc
     1441 tgccgggcat ggggttggtc tcccagatca ttcccgcttt ttcgcgcaaa ctcctcttcg
     1501 gctaccgggg aatggtgggg gccaccgccg ggatcggcat tatcggcttt ttggtttggg
     1561 ggcaccacat gtttgccacc ggcatcgacc cggccctccg cacctacttt tcggtgatga
     1621 ccatgctcat cgccctgccc accggcatca aagtcttctc ctggatcgcc accgcttggg
     1681 gcggcacgat ccgtttcacc acggcgatgc tcttcgcttt gggtttcgtt tcccttttcg
     1741 ttatcggcgg gatgagcggg gtgatcctcg ccaacattcc ggtggacgtg caggtgcacg
     1801 acacctactt cgtggtggcc cacctccact acgtcttgtt cggcggatcg ctcatggcgc
     1861 tcttgggcgg cttgttcttc tacttcccaa agatgaccgg ccggatgtta gacgaaaagt
     1921 tggggaaaat caccttctgg ctctcctaca ttgggatgaa cgtcaccttc ttccccatgc
     1981 actggctggg gattctgggc atgccccggc ggatttcttc ctaccccgac cgcccggcct
     2041 tcaccttcat taaccagctg gagactttcg gctacgtcat catcctggcg ggggcaatcc
     2101 ttctgctcta caatatgtgg cgttcgctca gacgaggggc ggcggcttct gccgacccct
     2161 ggcaagtgaa cgacattcag aagacgttgg attggactgt ctccagccca ccgccggtgg
     2221 aaaacttcaa ggaaattccg gtggtcacat gagcgcagaa gttgtgctga cccaaccggc
     2281 cgcaccgtcg gctggtgcgg aaggagagaa aattgctctc gcccgggcgc ggatcgcctc
     2341 gtggctggta gtgctggcct tagtcgtttt cgttttgatc ctgtggggag ggattgtccg
     2401 cctctccggg tcggggctct caatcccgga gtggcccatc gtcaacggga gtctcctgcc
     2461 gcccttttct tccggtgact gggatgcggt ttaccgcacg taccatcggg aaattttgca
     2521 cattaccgac ctagggtcgc cggaggccat tcccccgaga cgattcaagg cgatgttcac
     2581 cattgagtac cttcaccggt ttctggcgag tctgacgggc atcctctttt tggccatctt
     2641 cgtccgggct tggagattga aaccggtgcg ctcccaggtg aaaggtcttt tgatcaccgc
     2701 cgcattcttc ttggtggcac aagccgtact gggcggcatc gtggtcaagc gcgaactcga
     2761 ggccgaggtc gtcgccgggc acctgggttt agccttcctc tttttcgcca ccatcctgtg
     2821 gatggctttg cggcttttgc gcctgcccgc agctgcccgg gtgctgccgc agtcaaaact
     2881 cagacttctg gcctggagcg ctaccggcac ggttttcctg caaatcctct ccggcggcct
     2941 ggtcgccggt acccacgccg gttggatgat gaataccttc ccaaaaatgg gcgatttttt
     3001 ggtgccgccg ctttcagttc tctggtcctc catctacccc acccgctggc acaacttggt
     3061 tgagaatcaa gtgctcctcc agtttttcca ccgctggtgg gctttcgtcg ccacgcttgt
     3121 ggtcctgttt ttgattgtgc gggccattcc ctttcccctt tccccccgcg gccggctggg
     3181 gctccgtgcc gtcggctcgg tggtcgtact ccaggtactt ctggggatcg ggaatctctt
     3241 gatgcgggtg cccttttggc tttctctggt tcacctggcc actgccctct ttctcttcgg
     3301 tttgttagtg ctcatcaccc acgaactgcg ctaccgctat gaaacattcc ctcgctaatt
     3361 acctcaaact gaccaaaccg gagattttac tcctggtagt tctgaccggt gccactgcgc
     3421 tggtgatgga gggaagtctt ctcgcccacc cgctgcgctt cgggctggtg attttagctt
     3481 taattctgac cggcggctgc gccaatgcct tgaatcagta tttcgagcgc gacctcgaca
     3541 gccggatgct acgcacccgg cggaagcggc ctctgcccct gggggaaatt tcaccgcgcc
     3601 gggcgctcgt ctttgcactc gctctgggag ccgcagcgct tttgattttg ggaatggtgt
     3661 ttaatctttt cagcgccgtt ctggcgttaa tgacgattct cttttacagt ctcttctaca
     3721 ccctctggct caaaccccgc tcgcacctga acattgtgat cgggggtgcc gccggagcca
     3781 tggggccggt gattgcctgg gccgccgcca ccggctcgct atcgctcaca ccctggatcc
     3841 tttttctcat catcttcttt tggacaccac cccacttctg ggctttagcc ctctgtgtaa
     3901 aagaagacta cgcgaaagcg aagattccca tgttgccggt gatcaaaggc gatgaggaaa
     3961 ccagacgcca gattgtggtc tacaccctct ggctggttgg aatcagtttg gcgttacttt
     4021 tagtgaaagc cggcgcattc tccataaccc tggccttggc tctggggggc tttttcctct
     4081 ggaaggcggt gcaaaccttc cggcgccgca ctcaccgggc cgcctgggga ctgttcggct
     4141 attctatcct ctacctgtgg gtgcttttta tcgggatgat ggcggatgcg gtctggcacc
     4201 gcgctccgca ttgaggagaa gatgggaaga cgatgaataa gaaattgaag aagaacgtag
     4261 cgaccggact aatcttcgcc ggcgtggcgg tggggatgtt cgcctttgcc tttgccaata
     4321 tcccggtctt ccggctctac tgccaaaaag tcggcatcgc cctttcgccc aacgccaagg
     4381 ttaagcccgg tccggcggtt tccagcaacc gcgaagttca gattctcttc accggcgtag
     4441 tggccggcgg cgcgcccatc ttcttctcgg ccaaggaagc gtttatgacc gtccggctgg
     4501 gaaaagagac cgaaaccgcg taccggtttg tgaatctttc agccgacacg atccgcttcc
     4561 gtccggtgca cagcatcctg ccggaggcgg cggccaggcg cttttcactc aagaagtgct
     4621 tctgcttcga cgaccagacg ctattgccca agcaggaggt cgtgctgccg gtcatctttt
     4681 ccatcaatcc tgatcttgat ccggaggtgg agcaggtgac cttaaactac actctgtttc
     4741 ggaaaccgct ccacgggggg aattgatgtc ggaaaggaag cttgagcatt cgccgtggcc
     4801 gggcgccgat gtaacgggcc gtctcaagcg gagaaaccgc tggctgggag ttttcttcct
     4861 gctgttcgtt ctgggaatta ttgcactgag tttggtgatc tttagcaaac acggcttccc
     4921 gccgcactgg tgataaagag agggagcaat ggctgatatt gcacatacaa caaccgcggt
     4981 gcgccacctg gcgcacgccg aacacccgga gcacggcagc ccctggccca ttatcctagc
     5041 cggagggctg gggcttctgg ctgccggggt gctgaacctg atctggcaga ctaaatacgg
     5101 ttttcccttg ctggtgacgg gcggaaccat cgcccttctg gcggtcagca actgggcgca
     5161 caccctcatc cgggaaaaga attttctgga aaatttattc gtcgatgaga cctggatgaa
     5221 aaacgggatg aaacttttct taatctccga agcggccatt ttcggcgcct tctttgccca
     5281 ccactactac gcccgggtgc actccccggc ctggccgccg gtcggatcgc cgcatttgga
     5341 aacccggctg ccggccattg ccacgctggt tttgatggcc tccagcggca cctgccagtg
     5401 ggcgcacaac ctgctgcgga aagggaaacg cgtggccagc cagcgcggca tcctgctcac
     5461 gatggttttg ggtattctct tcttaggcgc ccaggggtac gagtggggct tcctcaaggc
     5521 ctacgatgat tttaccctca agaacggagc tttcggcacc agtttcttca ttatgaccgg
     5581 cttccacggc ctgaatgtga gcatcgggat catcctgctt tctatcgtct acgtccggct
     5641 gcgcctgggc cactttacgc cggagcgcca cttttcgatg atcgccgctt cctggtactg
     5701 gcacttcgtc gacatcgtct ggattttcct cttcgggacg atgtacttaa tctgagccca
     5761 ggcagtacgc agaaattatc aggcccgctc aatcaaagcg ggccttttct ttggcttcct
     5821 ggaggttaaa gtgagccggg ataatgaggg aatagcggcg agtggattcg aaccaccgac
     5881 acgcggctta tgaagccgct gctctaaccg actgagctac gccgccacag agggtagaat
     5941 cccatcaaaa gaaaagccaa aaaaagaaaa ctggcaaggg agctggccgt tacttaatca
     6001 cccggcgttt catggccacg agggccagga ggtaaaaaag gccgcaggca accagaagcc
     6061 agaggacgtt cagcgccaga tgccacgacc ccagaccggt ggtgagcgcg cgggagatgg
     6121 tggtggtgtg gtataaaggc ataaaccagg ccacggtgtg cgcccagccc gggagccgct
     6181 ccaccgggaa aaaggtcccg gcgaacagga acatcggggt catcaccagg gtgaaatagt
     6241 agtccatgta gttgaattcc gggaaaaagg aggtgtaggt catcgccagg gagccgtgaa
     6301 tgatgccgga tatgaaaatg acgaggggga gaagcaccac cagaggcgag tggatgagac
     6361 cgaagagcca gatgacgatt aaaatcgaca ctgcctggat gacgctgcgc gccgcgccga
     6421 agaggatttc gccggtgatg acgtcttcca ccgataccgg cgtatggatc atggcgtccc
     6481 aggttttctg ctccttcatc cgcacgagcg tcccgacgat gcactcaaac gatggcgccg
     6541 taagcgccac cgaggcggca atcgccggtg cgatgaactc cgggtaggag agccctttga
     6601 ttttggccac cagcgccccc aggccgtacc ccatcgccag aaggtagaag agcggatcga
     6661 agaagggcgg gatgaggttg ctcttggcgt ttttccagaa gacgtcccgg tgcctttgga
     6721 gcacccgcag gcaacggtag gagatgtcca gtgagccaag tttcatatca tcggtttcct
     6781 atcatagaac cggccttaag gccggttcgg gataacgatt ttttccgcgc tccggctatc
     6841 acga
//
LOCUS       DBNGZA010000022         4570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_852099, whole genome shotgun sequence.
ACCESSION   DBNGZA010000022 DBNGZA010000000
VERSION     DBNGZA010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_852099"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            578..1279
                     /gene="thyX"
                     /locus_tag="AC16FU_00355"
     CDS             578..1279
                     /gene="thyX"
                     /locus_tag="AC16FU_00355"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR2992864.1"
                     /translation="MLVEVKEPKIQTRPGVLSVELMAITPNPEQVIEMACRTCYLSFH
                     RYNPPASTEELIKKILRKGHHSVLEHAFATFRVKGGSRVFTHELVRHRHLSPSQESQR
                     YVQYGKTKPYEYVIPPTLENSPFKERYAQLASACADFYAEMVEGKIPKEDARYILPGG
                     TVSEIVISANFREWRHICEVRCQPFAHWEIRTVMLAILKILKREAPIAFWDFVVDEEK
                     KQATLLPPEPTNGKE"
     gene            1374..2459
                     /locus_tag="AC16FU_00360"
     CDS             1374..2459
                     /locus_tag="AC16FU_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992865.1"
                     /translation="MFLFTVLLSTFLLVTPTGDRYPELVMENGNYYLVFEGDKKMKVG
                     ERIMTGYDSNVSEFSPDSLYIAYTQNSGLGFESSGKNIYIFEVKIRKERMILCDWFGC
                     WSLGWLQKGDDLLLLIDQDAAESGIKRLVAYDPRTDLITYDTTFEEQSLSSFVTREWK
                     ENLFSYKNRTPRWRDVSLPLEPCQDLTGVCVSGENQPLFDPRLCTNWSAWMPDLPLDS
                     TDALSYYYVEKVTAFAVSPDHRWLAVSSKSASASANIVQDVKSRAPRYLRVLFGGKAG
                     LPYWSSDSRFVAFANEPRGGRPIVHLFDLEKRNNELVAQAMLPPSATVQNVIFGPDIE
                     ETFVEFRTKNKGITRVRMQPVNSNGKK"
     gene            2498..>4570
                     /locus_tag="AC16FU_00365"
     CDS             2498..>4570
                     /locus_tag="AC16FU_00365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR2992866.1"
                     /translation="MANGREAMTTTTKVQTGLTLERFFTQEGVHPYDTVTWEKRTALI
                     RGADGKVVFEQKEVEFPSFYSQLATNVVVSKYFRGKLGTPQRETSLKQVISRVVNTIR
                     DWGVKDGYFGTAKDAQIFADELTHLLLYQKAAFNSPVWFNVGIELTPQCSACFILSVE
                     DTMPSILEWIRTEGMIFKGGSGSGVNLSPLRSSKEHLAGGGLASGPVSFMRGADATAG
                     AIKSGGKTRRAAKMVVLNVDHPDVTEFIRCKAVEEQKAWALGNAGFDMSINGEAWKSI
                     QFQNANNSVRVTDGFMQAVESDTPWEMREVRNGKVIETVPARELLREIAECTHLCGDP
                     GLQYDTTINSWHTCPNSGRINASNPCSEYMHLDDSACNLASLNLMKFRTPEGDFNPDA
                     FARAVDVLILAMDIIVDNSSYPTEKITRIAKAFRELGLGYANLGALLMARGIPYDSDE
                     GRAAAAAITAILTGEGYRMSAQISEKMGSFAGFEKNRDAMLNVISKHRLSLDNVNAKL
                     LPEKLFAAAEKSWDQAYTQGKKHGYRNSQATVLAPTGTIAFMMDCDTTGIEPDIALVK
                     FKNLVGGGVMKIVNQTVPEALAYLGYSKDEVEEIVKYVEEKGTIEGAPHLKLEHIPVF
                     DCAFKAPGGHRSIHHLGHLRMMGATQPFISGAISKTVNMPKDSTVEDIMDAYLQGWKL
                     GLKALAIYR"
BASE COUNT         1067 a         1310 c         1189 g         1004 t
ORIGIN      
        1 cttctctctc tctgtagcgc aggttggttt ttgtaacctg cgccaaattt ccctcccctt
       61 gagggaaggg atgaagggag gggtcaacac ctcaaatcat attcaaaccc tgggcgaccg
      121 ggggcggtcg ctcctacgag cgcggtcggg gttttgtggt aaaccggtcg gcacaaccca
      181 tatgggctcg tcccggagcc ggtcaaataa ctcgcgtagg tgcgacgccc tcgtcgcgct
      241 cttctctata caaatgtata cccttgcgcg tccggactcg aactttcatc tcaccccaat
      301 tttttgtacc tctgtccctt tctcccacaa ccggttgtat attttcttta tcgagatgaa
      361 aaaaatactt gacaacttcc acctcccacc ctaacatacc ccccgctctg aaaaccttat
      421 gttcgtccgg ctgaagagaa aagtcctgct gttgtatcgc taagccctcc ccttcgcctc
      481 gggcggagaa tgaagggcaa ggacaattct ctttcctggt cgtattatct ttatggaaag
      541 cgcaaagacg acaaacttac actcagaaag gtcaacaatg ctggtagaag taaaagaacc
      601 gaaaatccag acccggcccg gggtgctctc agtggagttg atggccatca cgcccaaccc
      661 ggagcaggtg atcgaaatgg cctgccgcac ctgctacctc tccttccacc gctacaaccc
      721 cccggcctcc accgaagagt tgatcaaaaa aatcctccgc aagggccacc actcggtttt
      781 ggagcatgcc tttgccacct tccgggtcaa aggcggctcc cgggtcttca cccacgagtt
      841 ggtccggcac cggcacctct ccccttcgca ggagtcgcag cgctacgtcc agtacggcaa
      901 gaccaagccc tacgagtacg tcatcccgcc cacgcttgag aactccccct tcaaggagcg
      961 ctacgcgcaa cttgccagtg cctgcgccga cttctacgcc gaaatggtgg agggcaaaat
     1021 acccaaggag gacgcccgct acatcctgcc gggcggcacg gtgtcggaga ttgtcatctc
     1081 cgccaacttc cgcgagtggc ggcacatctg cgaggtgcgc tgccagccct ttgcccactg
     1141 ggagatccgc accgtgatgc ttgcgattct gaaaatcttg aagcgcgaag cgcccatcgc
     1201 cttctgggat ttcgtggttg acgaggagaa gaaacaggcg acgcttctgc cgcccgaacc
     1261 tacgaatgga aaggagtgac gctttcttta tttcgcgtag gtggggcttc agccccacga
     1321 tattcacccg cgtaggcgcg acgtccccgt cgcgctaaac tggcgtatga accatgtttt
     1381 tgttcacggt tttgttaagt acctttttgc tcgtcacccc aactggtgac aggtacccgg
     1441 aactggttat ggaaaatggc aattactatc tggtttttga aggtgacaaa aaaatgaagg
     1501 tcggagaaag aattatgaca ggatacgatt caaatgtttc agaattttca ccggacagtc
     1561 tgtatatagc ttacacacaa aacagtggtc ttggttttga aagctccggt aagaacattt
     1621 acatttttga agtgaaaatt cgcaaagaac gaatgattct ctgtgactgg ttcggctgtt
     1681 ggagccttgg ctggctacaa aagggtgatg atttgcttct actcattgat caggacgcgg
     1741 ctgaaagtgg cataaagcgg ttggttgcct acgatccgcg cacagattta atcacatatg
     1801 acacgacctt tgaggaacag agtttatctt ctttcgttac gcgcgaatgg aaggaaaatc
     1861 tcttctccta caaaaataga accccgcgct ggcgcgatgt ttccctacca ttagagccct
     1921 gccaagattt aaccggtgtc tgcgtaagtg gtgaaaatca gccgctgttt gatccacgtc
     1981 tatgtactaa ctggtcggca tggatgccgg atttaccttt agatagtact gatgcccttt
     2041 cttactacta tgtcgaaaaa gtaactgctt tcgcggtctc tcccgatcac aggtggcttg
     2101 ctgtctcgtc caagagcgcc tcagcctcag ccaacatcgt gcaagacgta aaatcccgcg
     2161 ccccgcgcta cctgcgggta ttattcggtg gtaaggccgg tctcccttac tggtcttccg
     2221 attctcgctt tgtcgctttt gccaatgaac cccgcggcgg gcgacccatc gtgcatcttt
     2281 ttgaccttga aaaaagaaat aacgagctcg tagctcaggc aatgctgcca cccagcgcaa
     2341 cggtacagaa tgttatcttc ggcccggata tagaagaaac ttttgttgag tttagaacca
     2401 aaaataaagg aattacccgc gtcagaatgc aaccagtgaa ttccaacggt aagaaatgag
     2461 tggacaaatt tgtcgataac tccagaggag ggaagaaatg gctaatggac gagaggcaat
     2521 gacgactacc accaaagtcc aaaccggctt gacccttgag cgcttcttca cccaggaagg
     2581 cgtgcatccc tacgataccg tcacctggga gaagcgcacg gctttaatcc gcggggcgga
     2641 cggcaaggtg gtcttcgagc agaaagaggt cgaattccct tcgttttatt cccagttggc
     2701 gaccaacgtc gtcgtttcca aatacttccg gggaaaatta ggcactcctc aaagggaaac
     2761 ttcactcaag caggtcataa gccgggtggt caatacgatt cgggattggg gcgtgaaaga
     2821 cggatatttt ggcacggcca aagacgctca aatcttcgcc gatgaactga cccatctcct
     2881 cctctaccag aaagccgctt tcaactcccc ggtctggttc aacgtcggca tcgagctgac
     2941 cccccagtgt tcggcttgct ttatcctctc ggtggaagac accatgccgt cgattttgga
     3001 atggatcagg accgaaggaa tgattttcaa aggcggttcc gggtcgggcg tcaatctttc
     3061 tcctctgcgg tcatccaagg agcacttagc tggcggcggt ctcgcctccg ggccggtctc
     3121 ctttatgcgc ggggccgacg ccaccgccgg cgcgatcaag tcgggcggca aaacccgccg
     3181 cgccgccaaa atggtggtct tgaacgtcga ccacccggac gtgaccgaat tcatccgctg
     3241 caaagcggta gaagagcaaa aggcctgggc tttggggaat gccgggttcg atatgtcgat
     3301 caacggcgag gcctggaagt caatccagtt ccagaatgcc aataactcgg tccgcgtcac
     3361 cgatggcttt atgcaggcgg tggaatcaga cacgccctgg gaaatgcgcg aagtgcgcaa
     3421 cggcaaagtg atcgagaccg tcccggcgcg ggaactcctg cgcgaaatcg ccgagtgcac
     3481 ccacctctgc ggcgaccccg gcttgcagta cgacaccacc atcaactcct ggcacacctg
     3541 ccccaattcg ggccgcatca acgcctccaa cccctgctcg gagtatatgc atctggacga
     3601 ctccgcctgc aatctggcga gtttgaacct gatgaaattc cgtaccccgg aaggcgattt
     3661 caatccggac gcctttgccc gcgcggttga tgtcttaatt ttagcgatgg acatcatcgt
     3721 cgataattct tcctacccga cggagaaaat taccagaatc gcgaaggcgt tccgcgaatt
     3781 aggtttgggc tacgccaatc tgggcgcgct tttgatggcc cgcgggattc cctacgactc
     3841 cgacgagggc cgggccgccg ccgccgccat caccgccatt ttgaccggcg aagggtaccg
     3901 gatgtcggct caaatcagcg agaagatggg atcgtttgcg ggttttgaga aaaaccgcga
     3961 cgcgatgctg aacgtcattt ccaagcaccg cctcagtctg gataacgtaa atgcgaagtt
     4021 attgccggag aaacttttcg ccgccgcgga aaaatcctgg gatcaggcct acacgcaggg
     4081 caaaaagcac ggctaccgca actcgcaggc gaccgtcctc gcccccaccg gcaccatcgc
     4141 ctttatgatg gactgcgaca cgacgggaat tgagcccgac atcgccctgg tcaagttcaa
     4201 aaacctggtc ggcggcgggg tgatgaaaat cgtcaaccag accgtgccgg aagcgctcgc
     4261 ctatctcggt tactcgaaag acgaggtgga ggagatcgtc aaatacgtgg aggagaaggg
     4321 caccatcgag ggtgcgccgc acctgaaact ggagcacatc cctgtctttg actgcgcctt
     4381 caaagccccc ggcggtcacc gctcgattca ccatctgggc cacctgcgga tgatgggcgc
     4441 cacccagccg ttcatttccg gcgccatttc caagaccgtc aacatgccca aagactcgac
     4501 ggtcgaagac ataatggacg cctaccttca gggttggaag ctgggcttaa aggcgctcgc
     4561 catttaccgc
//
LOCUS       DBNGZA010000023         3099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_427872, whole genome shotgun sequence.
ACCESSION   DBNGZA010000023 DBNGZA010000000
VERSION     DBNGZA010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_427872"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="AC16FU_00370"
     CDS             <1..1259
                     /locus_tag="AC16FU_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR2992867.1"
                     /translation="GADGFLLYQKDGSAAGDALGFGAAGAGDVNGDGSADFILGARGA
                     DPGGRSNAGSAYVYSGADGSLLYQKDGANAGAYLGASVAGAGDVNGDGRDDFIVGAGQ
                     AGGSGQAYVYSGADGSLLFEKNGGGTGQFSIAVAGAGDVNGDGKADFIVGSSLDDPVA
                     ESNAGSAWVYSGADGSLLYWKYGAHTGDFLGFAVAGVGDVNGDGKADFIIGAYAANPG
                     GVGNAGSAYLYSGADGSLLYQKDGAAANDFLGNSVAGAGDVNGDGRADFIIGASLADP
                     GGLFDAGSAYLYSGADGSLLFQKDGAAANDQLGMSVDGAGDLSGDGRAEIIIGAWRAD
                     PGGVQDAGSAFVYGLPCTGRRGDVGNSGSTDLEDIVALIQEVVFGTGLPNPLAGDTNC
                     DGVRDISDVVLLIQFVTFGTPQPCCL"
     gene            complement(1302..2480)
                     /locus_tag="AC16FU_00375"
     CDS             complement(1302..2480)
                     /locus_tag="AC16FU_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR2992868.1"
                     /translation="MPGRVEVRVHLFSTTSPFRVECYRGLKAKRTRTTDSASSGAVSR
                     YPDFGDALSWSSSEFYLDPQTAAVTLTPKGSPATVTIGEKLYRGRLEITKDLWNGLSV
                     VNVLEIEDYLKGVLPPEIGNRAENEFEAVKAQAVAARTYTLSKLNQYPGKNYDLEADT
                     RDQVYGGVTIETDLTNRAIRKTHGRVALYGGRLINAYYHANCGGCTDSISRIWAKPQE
                     DYLIPVEDSDFCRWSQTRLSEPNNYRWEERFPRSLLEHNFKTFLPPAYGKDTTGLLFI
                     DGLAVKSRTTGGRVDSLLVIWNAARGFDSTVLVRDSIRWAFRRASDTTRILRSTKFEV
                     SYVMDSSGLIDTVVVTGVGTGHGVGLCQTGAIELARRGYSYEKILQHYYPGIKIRRIY
                     "
     gene            complement(2666..>3099)
                     /locus_tag="AC16FU_00380"
     CDS             complement(2666..>3099)
                     /locus_tag="AC16FU_00380"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR2992869.1"
                     /translation="LYFHRKPPKTTGREMFGQEFVERCLSWRRKLKVSNADLIATVTE
                     LTAWSVWDACQKFIRPRLKIDELFVSGGGARNRHLLQRLGFYFSPVPVKSLTALGLDP
                     QALEAILFALLADFCVTGTSLPLRRITGAKKPVVLGKICQP"
BASE COUNT          604 a          822 c         1028 g          645 t
ORIGIN      
        1 ccggtgccga cggcttcctg ctctaccaga aagacggctc cgccgcgggt gacgcgctgg
       61 gcttcggcgc ggccggggcc ggggacgtga atggggatgg gagcgctgat ttcatcctcg
      121 gtgcccgtgg ggccgacccc ggcgggcgta gcaacgccgg ttcggcctat gtttattccg
      181 gcgccgacgg ctccctgctc taccagaaag acggcgcaaa cgccggcgcc tatctgggcg
      241 cctccgtggc cggagccggg gatgtgaacg gtgatggacg agacgatttt atcgtcggag
      301 ccgggcaggc tggcggtagc ggccaagcgt atgtctattc cggcgccgac ggctccctcc
      361 tttttgagaa gaacggcggc ggcacgggcc agttcagcat cgccgtggcc ggggcgggag
      421 atgtgaacgg ggatgggaaa gccgatttta ttgtcggctc ttccctggac gatccggtag
      481 cggagagcaa tgccggttcg gcctgggtct attccggcgc cgacggctcc ctgctctact
      541 ggaaatacgg cgctcacacg ggtgactttc tgggcttcgc cgtggccgga gtgggggatg
      601 tgaacgggga tggaaaggcc gatttcatta tcggcgcgta tgccgccaat ccgggcgggg
      661 tgggaaatgc gggctcggct tacctctatt ccggcgcaga tggctccctg ctataccaga
      721 aagatggtgc cgccgcaaat gactttctcg gtaactccgt agccggcgca ggagacgtga
      781 atggggatgg gagagccgat ttcatcatcg gcgcttccct ggccgacccg ggcgggcttt
      841 tcgatgccgg ttcggcttac ctctattccg gcgccgacgg ctcgctcctt ttccagaaag
      901 acggtgctgc cgcaaacgac caactgggca tgtccgtgga cggtgcgggg gatttaagtg
      961 gcgacggccg ggccgaaatc atcattggcg cttggagagc tgacccggga ggagttcagg
     1021 acgccggttc ggcttttgtc tacggcctgc cctgcaccgg gcggcgcggg gatgtcggaa
     1081 atagcgggtc gaccgacctt gaagatattg tagctctgat ccaggaggta gtgtttggaa
     1141 ctgggttacc taacccgctg gcaggagata ccaactgcga tggggtacgg gatattagcg
     1201 acgttgtcct gctcatccaa tttgtcacct tcgggacgcc gcagccgtgt tgtctgtgaa
     1261 agttgggatt taaatcaggt atccgtgaat agaaaaatca gttagtaaat tctgcggatt
     1321 ttaatcccgg ggtagtagtg ttgtaaaatc ttctcgtacg agtacccccg ccgagccagt
     1381 tcgatggcgc cggtctggca cagccccacc ccgtggccgg taccgacgcc ggtgacgacg
     1441 acagtatcga taaggccgct ggagtccatc acataactca cctcgaactt cgtgctgcgc
     1501 agaatgcgag tggtgtccga ggcgcggcgg aaagcccagc gtatggagtc gcgcactagg
     1561 acagtcgagt caaatccccg cgcggcgttc caaatgacca gaagcgagtc gacccgaccg
     1621 ccggtggtgc ggcttttgac cgcgaggcca tcgataaaga gcaagccggt cgtgtctttc
     1681 ccgtaagccg ggggcaggaa agtcttgaag ttgtgctcta aaagggagcg cgggaagcgc
     1741 tcttcccagc ggtaattgtt cggctcggag agtcgagtct gcgaccagcg gcagaagtcg
     1801 ctatcctcaa ctggaatgag gtagtcctcc tgcggtttgg cccagatgcg ggagatagaa
     1861 tcggtacacc cgccgcagtt ggcgtggtag taggcgttga tcagccgtcc cccgtagagc
     1921 gccacgcggc cgtgggtttt tctgatcgcc cgattggtca gatcggtttc gatagtcacg
     1981 ccgccgtaca cctggtcgcg ggtgtcggct tcgagatcat aattctttcc cggatactgg
     2041 ttcaattttg agagcgtata ggtgcgggcg gcgaccgcct gggctttgac cgcttcgaat
     2101 tcgttttcgg cgcggttgcc gatttccggc ggcaggacgc ccttcaggta atcctcaatc
     2161 tcgagaacat tcaccacgct caaaccattc cacaggtctt tggtaatctc cagccggccg
     2221 cggtagagtt tttcgccgat cgtgacggta gcgggactgc ctttgggggt aagcgtcact
     2281 gcggcggtct gcggatcgag gtagaattca gacgacgacc agctcaaagc atcaccaaag
     2341 tcaggatagc gggaaaccgc tccgctcgaa gcggagtcgg tggtgcgggt tcgttttgct
     2401 ttcaagcccc ggtagcattc aacgcggaag gggctggtgg tggaaaagag atggacgcga
     2461 acctcgacgc gaccgggcaa aggcggcgga ggtaaaggca cggcctgcgg ttgcggttgg
     2521 ggtgcaggcg ggacttcccc cggaggtgtt ggtgttggcg gcgggggcgg cggcaagcgc
     2581 cgagcacaac ccgccaacaa atttaaggca atgaataatg aaaagagtga tttaactcca
     2641 cgctttttta agaggattgt cctcatcacg gctggcagat ttttcccaaa actacaggtt
     2701 tttttgcgcc ggtgattcta cggagcggca gggacgtgcc ggttacgcag aaatcggcga
     2761 gtagagcaaa gaggatggcc tcgagggctt gaggatctaa tcccagagca gtaagacttt
     2821 tcaccggtac gggggagaag tagaaaccga gccgttgcag caagtgccgg ttacgggcgc
     2881 cgccgccgga gacgaaaagc tcgtcaattt tcagtcgcgg gcggatgaat ttttggcagg
     2941 cgtcccagac tgaccaagcg gtaagttcag tgacggtggc gatgagatcc gcattggaga
     3001 ctttcaattt cctgcgccag gataagcagc gttcaacaaa ttcttgcccg aacatttctc
     3061 gtccggtggt tttgggcggt ttccggtgaa aatagaggt
//
LOCUS       DBNGZA010000024         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1064517, whole genome shotgun sequence.
ACCESSION   DBNGZA010000024 DBNGZA010000000
VERSION     DBNGZA010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1064517"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="AC16FU_00385"
     CDS             complement(<1..924)
                     /locus_tag="AC16FU_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992870.1"
                     /translation="MGQRLLLPLKQLLNQLPGRFGRVSYEAEVKLLLLFLLLFTTLLG
                     FTSDYLLLKINQTWNRSQPGRQAVLEEWLSTHWERQPPQNSRDLQQLVRDLVVFTGPA
                     EVTFIPGKDSLLPPLRFPDPPLAGGNKGDQIVFTLPGKQGSLTLWLPANVAGVPVGLV
                     HWLWLLKIIAVGLLLVFLPLLWRYIFAPLKTISTQVQALPVPVPPRHPKSDAEVAIAS
                     FQQMIAELKTQQAELAALNALLHDRSHRLAKYNDQILRQMNLIAVICDAEGTVTHWNH
                     RAESTFDLAANRVIDQPFQQALRSVPAVVAVI"
     gene            1379..1936
                     /gene="efp"
                     /locus_tag="AC16FU_00390"
     CDS             1379..1936
                     /gene="efp"
                     /locus_tag="AC16FU_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR2992871.1"
                     /translation="MLSTTDFRKGLRMKFDGELYQIVDFQHARTAQRRAFVRTKLKSL
                     RTGAVLERTFAAGEQFDDPDFEEKAMQYMYTAGDEYFFMDQKTFEQVSLSAEKLGDAR
                     WYLKESEEYPILFFEGQPLSVDLPASVILRITATEPGVKGDSVANLTKNATLETGLVV
                     KVPLFVKEGERIKVDTRTGDYLERA"
     gene            1929..2648
                     /gene="rnhC"
                     /locus_tag="AC16FU_00395"
     CDS             1929..2648
                     /gene="rnhC"
                     /locus_tag="AC16FU_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIR2992872.1"
                     /translation="MPDVESAERLTIRHDKSVVALYFPDTVTERKESLIGTDESGKGD
                     YFGPLVVAAVFWDERAAEALAGLRIRDSKLLSDRRAAVLAREIVRTTRHAVVIIGPEK
                     YNELIARMKNLNRLLAWGHARAIENILAAAACPRAVSDQFGDEKFLNNALLEKGRRIE
                     LVQKTKAESEPAVAAASVLARNTFLQYLDKLSERAGIPLPKGAGPPVDQAAQALVGKW
                     GKEALGQFAKLHFKNTARILG"
     gene            2645..>3542
                     /locus_tag="AC16FU_00400"
     CDS             2645..>3542
                     /locus_tag="AC16FU_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005851 - eukaryotic translation
                     initiation factor 2B complex [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eIF2B alpha/beta/delta subunit family protein"
                     /protein_id="HIR2992873.1"
                     /translation="MNWQSELEKLCADNLSGSSALARKSGELLRSFARQECRHSGLEV
                     SKSFQSLAERLIRRFPEMASVEALLDQVALRLETKGGNSAFDLTRLVATEVSQFFRKQ
                     EIALAAIAEKAAAFFTKQVIVVTLSASMAVERMLIHLHAEGKLAGLFVLESRPLGEGM
                     LLAKRLAERGISVTAAVDAALDRVLEKADVALVGADCIDGQVVVNKIGTKLLALAARA
                     QAKPCYVAADTSKFGAGLLTSTSETQGQPDEVAGDLPAKVAVYNPYFEPTPLAWFTGI
                     FSEQGLLTLLQVGEVCARRPQSG"
BASE COUNT          768 a          977 c         1098 g          699 t
ORIGIN      
        1 aatgacggca acgaccgcgg gaacactgcg cagggcctgt tggaagggct gatcaatgac
       61 ccgattcgcg gccaggtcga aggtgctctc ggcccggtga ttccagtggg taaccgtccc
      121 ttccgcatcg cagattaccg ctatcaaatt catctggcgc agaatttgat cattgtactt
      181 cgccaggcgg tgcgaccggt catgcaggag ggcgttgagc gccgccagtt ccgcctgctg
      241 ggtcttgagt tccgcaatca tttgctggaa actggcgatg gcgacttcgg catcactctt
      301 ggggtgccgg ggaggcacgg gcaccggcag cgcctgcacc tgggtagaaa tcgttttcag
      361 cggcgcaaaa atgtagcgcc acagaagcgg gagaaagact aaaaggaggc cgacggcaat
      421 gattttcaga agccagagcc agtggaccaa tcccaccgga acgccagcga cgttagccgg
      481 caaccagagt gtcagcgacc cctgtttgcc cgggagggtg aagacgatct ggtctccttt
      541 attcccaccg gccagcggcg ggtcggggaa gcggaggggc gggaggagcg aatctttccc
      601 ggggatgaaa gtgacctctg cgggaccggt gaaaacaact aagtcacgta ccaactgctg
      661 gagatcgcgg ctgttctgcg gcggctggcg ctcccagtgg gtggagagcc attcctccag
      721 aaccgcctga cgtcccggct gcgaccggtt ccaggtctgg ttaattttga gcaggaggta
      781 gtcgctggta aaccccagga gggtagtaaa gagcaaaagg aacagaagca gcagcttcac
      841 ttccgcttcg tacgacacgc gaccgaagcg gcccggcagt tggttcaaca gttgtttcaa
      901 tggtagcagg agacgttggc ccatgggtcg atcactactt tcacgtatgg tctctcagcc
      961 cgtgaaaccg gcagggtgat ggttaaaggg tgatctggcc tgcgccgcca ccctatccga
     1021 taccaatcca gaacacctct tttgcaacca cgatgccggg ggctcaggtg tgggctgttc
     1081 aggttgtcag ttcgaacctc aagggaattg acaagttcaa cggcttcccg gtcaaggaac
     1141 cggggagcac gacacccgtt tcctctgtat ctggaaaaag tgagggaatt gctccatagg
     1201 tatgcggcgg gaggcggtcc gacctgtttg gacaactctg aagaaaagct tcaattgtcc
     1261 acctgccagc caggggagat gtccgggttt tcctcttgcc ggattgggaa atgagggtat
     1321 attgtgtaca gtccctgatc ctgtttcagg gattttgatt gtcggagacg accagaaaat
     1381 gctttccacc accgatttcc gcaaagggtt gcggatgaaa ttcgacggcg aactctacca
     1441 gatcgtcgac ttccagcacg cccggaccgc gcaacggcgc gcgttcgtcc gcacaaagtt
     1501 gaaaagtctc cgcacgggcg cagttctgga gagaaccttc gctgccggag aacagttcga
     1561 cgaccccgac tttgaagaga aggcgatgca gtatatgtac accgccgggg atgagtactt
     1621 cttcatggat cagaagacgt ttgagcaggt gagtctgtcg gcggaaaagc tgggcgacgc
     1681 ccgctggtac ctgaaggagt cggaggagta cccgatcctc ttcttcgaag gccaaccgct
     1741 gagcgtggat ctgccggcgt cggtcatttt gcgcataacc gcgaccgaac cgggggtcaa
     1801 gggcgactcg gtcgccaatc tgaccaagaa cgccacgctg gaaaccggtc tggtggtgaa
     1861 ggtcccgctc tttgtcaaag aaggcgaaag gatcaaagtc gacacccgga ccggcgatta
     1921 cttagaacgt gcctgacgtc gagtccgccg agcgcttgac gatacgccac gacaaatcag
     1981 tcgtggcgtt gtattttccg gataccgtga ccgagcgaaa agagagtctc atcgggaccg
     2041 acgaatcggg caagggcgat tacttcgggc ctctggtagt ggcagcggtc ttctgggatg
     2101 agcgggctgc cgaagctttg gcgggactgc ggatcagaga ttccaaactg ctttccgacc
     2161 gccgcgctgc ggtactggcc cgcgagattg tccggacgac ccggcacgcc gtggtgatca
     2221 tcggaccgga gaaatacaac gaactgattg cccggatgaa aaatttgaac cgcctcctgg
     2281 cctggggaca cgcccgggcg attgagaaca ttctggcggc tgccgcctgc ccccgggccg
     2341 tcagcgacca gttcggcgat gaaaagtttc ttaataatgc cctgctggaa aaaggacggc
     2401 ggatcgaact ggtccagaaa acgaaagccg agagcgagcc ggcagtcgcc gcggcttcgg
     2461 ttctcgcgcg caacactttt ctccaatatc tcgataaact ttcggaacgg gccggaattc
     2521 ccctccccaa aggggccggc ccgccggtcg accaggcggc gcaggcgtta gtggggaagt
     2581 ggggaaagga agcgctgggg cagttcgcaa aacttcactt caaaaatacc gcgcgcatcc
     2641 ttggatgaac tggcagagcg agttagaaaa actttgcgcc gataatcttt ccggttccag
     2701 cgcgcttgcc cggaaatcgg gcgaactgct ccggtccttc gcccgtcagg aatgccggca
     2761 ttccggcctc gaggtcagca aaagcttcca atccctggcc gagcggctca tccgccgttt
     2821 tcccgaaatg gcttcggtgg aggcgctgct cgatcaggtg gccctgagac tggaaacgaa
     2881 aggagggaat agtgcctttg acctcacccg actcgtcgcc accgaagtgt cgcaattttt
     2941 ccgcaaacaa gaaatagccc tggccgctat cgccgaaaaa gcagcggcct tcttcaccaa
     3001 acaggtgatc gttgtgactt tgagtgcttc tatggcggta gaacgaatgc tcattcattt
     3061 gcatgcggag ggcaagctgg cgggtctgtt cgttctggaa tcacgaccgc tgggcgaggg
     3121 gatgctcctg gccaaacggc tggcggagcg aggcatttcg gtcacagcgg ccgtcgatgc
     3181 cgcgttggac cgggtccttg aaaaggcgga tgtggcgctg gtcggcgccg attgcattga
     3241 cgggcaggtc gtggtcaata aaatcggaac gaaacttttg gccctggcgg cgcgggctca
     3301 ggccaaaccc tgctacgtcg cagccgacac gagcaagttt ggcgccggtc ttttgacctc
     3361 taccagcgaa acgcaagggc aacccgatga agtggccggt gatctgcccg ccaaggttgc
     3421 cgtatacaac ccctattttg agccaacgcc tctggcctgg tttaccggaa tcttctctga
     3481 acagggactc ctcactttgc ttcaggtggg tgaggtctgt gcccggcggc cgcaaagcgg
     3541 tt
//
LOCUS       DBNGZA010000025         2649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1594887, whole genome shotgun sequence.
ACCESSION   DBNGZA010000025 DBNGZA010000000
VERSION     DBNGZA010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1594887"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC16FU_00405"
     CDS             complement(<1..284)
                     /locus_tag="AC16FU_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992874.1"
                     /translation="MVYGTLGAQEIDLLTSESIPSAQRLLFRSVVLGGIDQTVFYGTL
                     VDTRGNFLPATLSSPKVIVLPKSNAQVIVAGPESNVSFRLARTDAGADPAV"
     gene            complement(534..1367)
                     /locus_tag="AC16FU_00410"
     CDS             complement(534..1367)
                     /locus_tag="AC16FU_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992875.1"
                     /translation="MALTNIELVKKHLRERIVPGPQVENVPVKPVGTAASQLPNTSLL
                     ANSEKVKAQEATQPTFEQVALTSAGVPLLHPEIVPDSVVVAKDSSLAQVYVENVDYAV
                     DYDAGKIKRIGAGSITEGQSVAVWYFFFRLYTKGVDYTIDYGKGKLTRLSSGAIEDGQ
                     VVLCDYSLDLAALGDDVIANAILEAEDRVTKFVDPTYLATEDQSLVTGATYWAVAVLC
                     NSQSLEAMQASAASGVQARALSRSWSIMANVYNRRGLEMLTPFARPQPHFASPRAVTG
                     R"
     gene            complement(1549..>2649)
                     /locus_tag="AC16FU_00415"
     CDS             complement(1549..>2649)
                     /locus_tag="AC16FU_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992876.1"
                     /translation="GAVQSIYNNDQNYHPFIFKDKIDRLWVVWSRFITASSDYRIQVK
                     SSTNDGATWGTGPTDPGTALSSAGSSAYGQLIALGEKIYAFYTIGGTTTALRSFDISG
                     AVWSAEQVAYSGTGLSSDFHAATSADQKIGLLFNSSAGLLFKEYDGTNFSGQFTVDAA
                     PTTTPQLVYFQSTPYALFAKNIGTDQNRALYAYRNGSNFTAAQALFGDAVPFVKVFCY
                     DQSAVAKFADRTTEAGNTAAADVFHPTSGGLVKDPQDALYVGLDVPFNQIAVLLSTTG
                     IAGQVIFSYWDGSAWVEFTPDSGAYHFATSPALVRLWPDLESVPATWRKSAVNSSVQF
                     WIKIEVQTAFTTVPVGTQITAVPNLKYLSAGG"
BASE COUNT          525 a          697 c          860 g          567 t
ORIGIN      
        1 accgccgggt cggcgccggc atcggtgcgg gccaggcgga aggagacatt ggattccggc
       61 ccggccacga tcacctgcgc attcgacttg ggcaggacga tgaccttagg gctggaaagt
      121 gtcgccggca gaaaatttcc tctggtatcg acgagcgtgc cgtagaagac ggtttgatcg
      181 attcctccca gaaccaccga gcgaaagaga aggcgctgcg cggaggggat ggattcactc
      241 gtcagaagat cgatttcctg cgcgccgagg gtgccgtaga ccaccagtcc tgggacagct
      301 cagaggcgac gtccgcggag gtaggcgagg gtggtgttca taaaatcctc attcgtgtcg
      361 aagtgtcggg gtatcgaggt gtcggagttt tcgtagttcc gggattacca aagcaatcag
      421 acttagaccc ctccctgccc tcccctcaag gggagggaaa atcctgggtg caccattcgg
      481 gcgcgacggg ggcgtcgcgc ctacgcgggt ttggaatggg tggccagaca ttctcacctt
      541 ccagtcaccg cccgggggct ggcgaagtgc ggctggggtc gggcaaaggg cgtcagcatt
      601 tccaggccgc ggcggttgta gacattggcc attatggacc aggaacggga aagcgctctg
      661 gcctgcacgc cggaagcagc ggaggcctgc atcgcttcca gggattggct gttgcacaaa
      721 accgccaccg cccagtaagt ggcgccggtc accaacgatt gatcttcggt ggcgagataa
      781 gtcggatcga cgaatttcgt cacccgatct tcggcttcga ggatcgcatt ggcaatcacg
      841 tcatcgccca aggcggcgag atcgagtgaa tagtcgcaga ggacgacctg gccatcttca
      901 atggcgcctg aagataggcg ggtcagtttg cctttgccgt agtcgatagt gtaatccact
      961 cctttggtgt agagacggaa gaagaaatac cagacggcaa ccgattggcc ttcggtgatg
     1021 cttccggcac caattctttt gatttttccg gcgtcgtaat cgacggcgta gtccacgttt
     1081 tctacgtaca cctgcgccag ggagctgtct ttggcaacga cgacggaatc ggggacgatt
     1141 tccggatgaa gcagtggcac accagccgaa gtgagcgcaa cctgttcaaa ggttggttgc
     1201 gtggcttcct gcgctttcac cttttcggag ttggcgagga gcgaagtgtt cggcagctgg
     1261 ctggcggcgg tgccgaccgg tttcaccgga acgttctcca cctgcggccc ggggacgatg
     1321 cgctcgcgga ggtgtttttt gacgagttcg atgttggtta aggccatatg ttaagtgtcg
     1381 aggtgtcgaa gtgtcggggt gtcgaagtta aaaattcttt tgacccctcc ctgaccctcc
     1441 cctcaaaggg gagggaaaat tccttcaaat ctttcccgtg cagattacag aaaccaaccc
     1501 cgccagaggc gggatcttcg acctgcacta cgttggtatt caaatctctc atcctcctgc
     1561 gctcaggtac ttgagattcg ggacggcggt tatctgcgtt cccaccggga cggtggtgaa
     1621 agcggtctgt acttcgattt tgatccagaa ctggacgctg gagttgaccg cactcttgcg
     1681 ccaggtggcc ggcacgctct ctaaatcggg ccagaggcgc accagcgcgg gtgaggtggc
     1741 gaagtgatac gcccctgaat cgggagtgaa ctccacccag gcggaaccgt cccagtaact
     1801 gaaaatgacc tgcccggcga tgccggtggt ggagaggaga acggcaatct ggttgaaggg
     1861 cacatccagc ccgacgtaaa gcgcatcctg cgggtctttg acgagcccgc cggaggtggg
     1921 atgaaacaca tcagctgcgg cggtgtttcc cgcttcagtt gtgcggtcgg cgaatttcgc
     1981 cacggcgctc tggtcgtagc agaagacttt gacgaagggt accgcgtctc cgaagagcgc
     2041 ctgcgcggcg gtgaagttac ttccgtttcg gtacgcgtag agcgcccggt tttgatccgt
     2101 cccgatgttt ttggcgaaga gggcgtaggg agtgctctgg aagtagacga gttggggagt
     2161 cgttgtcggc gcagcgtcaa ctgtgaactg gccgctgaag ttcgtgccgt cgtattcttt
     2221 gaacaaaagt ccggcgctgg aattgaagag gaggccgatc ttctgatccg ctgaggtggc
     2281 ggcatggaaa tcgctggaaa ggccggtgcc agaataggcc acctgttccg cgctccagac
     2341 cgcgccggag atgtcgaagg aacgcagcgc tgtcgtcgtg ccgccgatgg tgtagaaggc
     2401 gtagattttt tcgccgagag caatcagttg tccataggcg gaactgccgg cgctgctcaa
     2461 tgccgttccg gggtcggttg gtcctgtgcc ccaggtggcg ccgtcattgg ttgacgattt
     2521 gacctgaatg cggtagtcgc ttgacgccgt gatgaagcgc gaccagacca cccagagccg
     2581 gtcgattttg tccttgaaga tgaagggatg gtagttctgg tcgttattgt aaatgctttg
     2641 aacggcgcc
//
LOCUS       DBNGZA010000026         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2018942, whole genome shotgun sequence.
ACCESSION   DBNGZA010000026 DBNGZA010000000
VERSION     DBNGZA010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2018942"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(471..890)
                     /locus_tag="AC16FU_00420"
     CDS             complement(471..890)
                     /locus_tag="AC16FU_00420"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2992877.1"
                     /translation="MKIRNATTTDFPFIQRSIKEWLLDYENLTPEQFIVVEEDDRILG
                     FGRLKPYPDGTVELGCVGVIPERRKQGIGEMITNELIRRGPDTIYITTDLSNYFVPFG
                     FKLTSEYPEAIKKKLAWCETGTCRPGVVPMVLKKGNK"
     gene            complement(919..2472)
                     /locus_tag="AC16FU_00425"
     CDS             complement(919..2472)
                     /locus_tag="AC16FU_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR2992878.1"
                     /translation="MTTPIEEKIAYLKKLRAEAKLGGGEKRIEEQHRKGKLTARERLE
                     ILLDENSFEEFDAFVTHQTTAFGLDKQKYLGDGVVTGYGTIYGRPVFVFSQDFTVFGG
                     SLSGAYAEKICKIMDLAMKVGAPVIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGV
                     LPQISVVLGPCAGGAVYSPAITDFIFMNRTAYMFVTGPSVVKTVTHEEVDSEALGGAQ
                     VHATKSGVAHVVGENEADTLVKVRQLMRFLPQNNVDDPPVLPSDDPADRMEESLNTLV
                     PENPNKPYDIKDVIRRVADHGEFFEIQEQYAQNIVVGFARFGGKPVGIVGNQPAVLAG
                     VLDIDSSLKGARFVRFCDAFNIPLVTFVDVPGFLPGTVQEWGGIIKHGAKLLYAFAEA
                     TVPKITVITRKAYGGAYDVMSSKHIRADLNYAWPTAEIAVMGPKGAVEILFKKEIAES
                     KDPEKALEEKVAEFREKFANPYTAAERGYVDDVIEPKETRPRIIRALQMLETKKDTNP
                     PKKHGNIPL"
     gene            complement(2479..>3250)
                     /locus_tag="AC16FU_00430"
     CDS             complement(2479..>3250)
                     /locus_tag="AC16FU_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2992879.1"
                     /translation="GVVPKTMWSKLIPADERNLIPMDENIFLVKAHGKNVLLDVGLGD
                     VLSDRDRKVYNCYTPSRLEDGLKAIGLAPDDVDFVLLTHLHTDHCGGAWKLDEKGKVV
                     PRFRKARHIIQKSEWEDALHPDERTSAVYLADRLRVLGETNLVDLVDGEGQVIPGITI
                     TRTGGHTRGHQAVLVESDGHRLVAYADVIPTTSHLRPAYVASVDIFPLDTMKVKKQVI
                     PRLVQDGTLVAFDHDLKIKIGRIVERGDKLVAESVTLA"
BASE COUNT          670 a          938 c          884 g          758 t
ORIGIN      
        1 agtttgacta attggtcgat accgaacagc atctgggttg ctttcggatg atcttgtttt
       61 gttccagaga taagtgtaat caacaccgag atggattctc tctgccaccc gtaatccata
      121 ctgcccccta agattaacat catactgccg acctggaaaa atatcattag gtctggaagt
      181 ctcattgact ttccatgttt gagagtaggc gagaactcca taccgagata agggcaaaac
      241 cccgctcaac caaaaatcaa cctgattggc aaattcacca ctgacgttac ccctgctaag
      301 catagcaagg taagccggat ttcgaaaaat gtccgtcctt tcgtccggga ccagtccgat
      361 aaagtctgtc cccagagcac gtatgcggag tgaaggattc aaatacgcac cggcaaggcc
      421 tgggcaaacc acaataaaca taaagattac gaaacacttt cccagtgctt tcacttgttt
      481 cctttcttca gcaccatcgg cactacgccc ggacggcagg tgccggtttc gcaccaggcc
      541 agtttctttt tgatcgcttc cgggtattct gaggtcagtt tgaaaccgaa tggaacaaag
      601 tagtttgaca ggtcggtggt gatgtagatc gtgtccggcc cccggcgaat cagttcgttg
      661 gtgatcattt ccccgatacc ctgcttgcgc ctttccggga tcactcccac gcaccctaac
      721 tccaccgtgc cgtcgggata cggcttcagc cgcccgaagc ccagaatccg gtcatcctct
      781 tctactacaa taaactgttc aggcgtaaga ttctcatagt caagcagcca ctccttgata
      841 gagcgttgaa tgaatgggaa atctgtggtc gtggcgttgc ggattttcat cttatacgac
      901 ctgggtgaag gagatctctt acaacggtat attcccgtgc ttcttcggcg ggttggtgtc
      961 ctttttggtt tctaacattt gcaatgcgcg gatgatgcgc gggcgcgttt ctttcggctc
     1021 gatcacgtcg tcaacgtacc cccgctccgc cgccgtgtag gggttggcaa atttttcgcg
     1081 gaattcagcg actttctctt ccagcgcctt ctccgggtct tttgattcgg cgatttcttt
     1141 tttaaagaga atctccacgg cccctttggg ccccatgacg gcgatttccg ccgtcggcca
     1201 ggcgtagttg aggtcggccc ggatgtgctt ggacgacatg acgtcgtacg ccccgccgta
     1261 ggccttgcgg gtgatgaccg tgattttggg caccgtcgcc tcggcgaaag cgtagagcag
     1321 ctttgccccg tgcttgatga tcccgcccca ctcctggacc gtgccgggca gaaacccggg
     1381 aacgtccacg aaggtgacga gcggaatgtt gaacgcgtcg caaaaccgca cgaagcgggc
     1441 gcctttgagc gaagagtcga tgtccagcac gccggctaag accgcaggtt ggttgccgac
     1501 aatccccacc ggctttccgc cgaagcgcgc aaaaccaacg actatgtttt gcgcatactg
     1561 ttcctggatt tcgaaaaatt cgccgtgatc ggccacccgg cggatgacgt ccttgatgtc
     1621 gtacggtttg ttcgggttct cgggcacgag cgtgttgaga ctctcttcca tccggtccgc
     1681 cggatcatcg gatggcagaa ccggcgggtc atcgacgtta ttctggggca aaaagcgcat
     1741 cagttgccgg actttcacca gcgtatcggc ttcattttcg ccgaccacgt gcgccacgcc
     1801 cgacttggtg gcatgcacct gcgccccgcc caacgcttca gaatcgacct cttcgtgcgt
     1861 caccgttttg accaccgagg gcccggtgac gaacatgtag gcggttcgat tcatgaaaat
     1921 gaagtcggtg atggccggcg agtagaccgc gcccccggcg cagggaccga gaacgaccga
     1981 aatttgcggg aggacgccgg aggcaagcgt attgcgcagg aagatgtcgg cgtagccgcc
     2041 cagactcacc accccttcct gaattcgcgc cccgccggag tcgttcaatc cgatcaccgg
     2101 cgcgcccacc ttcatggcca gatccataat cttgcagatt ttctcggcgt aggcgccgga
     2161 gagcgaaccg ccgaaaacgg tgaaatcctg ggagaaaacg aaaaccggac ggccgtagat
     2221 ggtgccgtag ccggtcacca cgccgtcgcc cagatacttc tgcttatcga gtccgaacgc
     2281 ggtagtctgg tgggtgacga aggcgtcgaa ctcttcgaac gaattttcgt ccaaaagaat
     2341 ttcgagccgt tcccgggcgg tcagtttccc cttccggtgc tgctcctcaa tccgcttctc
     2401 ccccccgccc aatttcgctt cggcgcggag ttttttgaga tacgcgattt tttcttcaat
     2461 aggcgtggtc ataggaagtc aggcaagagt gactgattcg gcaaccagtt tgtcgccgcg
     2521 ttcaacgatc cgtccgattt tgatcttcaa gtcgtggtca aaagctacta aggttccgtc
     2581 ctgtaccagc cgcgggatta cttgcttctt gactttcata gtatccagcg ggaatatatc
     2641 gaccgacgcg acgtacgccg gccggagatg cgaggtggtg gggatgacgt cggcgtaggc
     2701 caccaaccgg tggccgtcgc tttccaccag aaccgcctgg tgcccgcgcg tatgcccgcc
     2761 ggtccgggtg atagtgatgc ccggtatgac ctgcccttcg ccatcgacta agtcaactaa
     2821 attggtttcc cccaacacgc gcaaccgatc ggctaagtag acggcggacg tgcgttcgtc
     2881 ggggtggagg gcgtcctccc actcggattt ctggatgatg tgccgggcct tgcggaatcg
     2941 cgggacgact tttccttttt cgtctaattt ccaggcgccg ccgcagtggt cggtgtgcag
     3001 gtgcgtgaga aggacgaaat caacgtcatc gggcgccagt ccgatcgctt tcaatccgtc
     3061 ttcaagtcgc gacggcgtgt agcagttgta aactttccga tcgcggtcag aaaggacatc
     3121 ccccagcccg acgtctaaga gaacgttctt cccgtgcgcc ttgaccagga aaatattctc
     3181 atccatcggg ataagattcc gttcgtcggc gggaatgagt ttcgaccaca tcgtcttggg
     3241 caccacgccg
//
LOCUS       DBNGZA010000027        17530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_110875, whole genome shotgun sequence.
ACCESSION   DBNGZA010000027 DBNGZA010000000
VERSION     DBNGZA010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_110875"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC16FU_00435"
     CDS             complement(<1..393)
                     /locus_tag="AC16FU_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR2992880.1"
                     /translation="MAVATPEKKPGKTGPSPGIKLLLPQTIQKMRQALPKFPVRRSAI
                     LPALHAAYDQLGYLNDEVYAEIGQVTGIPAVEIAESATFYTLFPKKKVGKYLIMVCTN
                     ISCALCGADSLVSYLERKLGIKNGETTPD"
     gene            complement(393..1565)
                     /gene="nuoD"
                     /locus_tag="AC16FU_00440"
     CDS             complement(393..1565)
                     /gene="nuoD"
                     /locus_tag="AC16FU_00440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR2992881.1"
                     /translation="MLKTETMTLNMGPQHPSTHGVLRVILELDGETVIKATPEIGYLH
                     TGIEKNAEDKKYYTALPMTDRMDYAAPMSNNLGFCLAVEKLIGMEIPPKAQYLRVLLT
                     ELTRIKSHLLWIGTHAIDIGAMSMFLYAFRERERVIEIYELVSGQRMMSTYFRVGGVV
                     QDVPPDFEPKIRAILRDFPKRFDEYENLLTNNPIWRNRTIGVGVIAAKDAIDAGLSGP
                     SLRGCGVKWDIRKSNPYSGYEKFDFEIPTGKNGDVYDRYLVRIAEMRQSLRIIRQALD
                     GMPEGDVMSRMPGFTPPPKREVLYSMEALIFQFKYWTEGFRPPVGEVYQAIESPKGEL
                     GFYFVSDGSPKPYRMHVRAPSFVNLSALSKLVEGRLVADVVATIGSIDIVLGEIDR"
     gene            complement(1648..2157)
                     /locus_tag="AC16FU_00445"
     CDS             complement(1648..2157)
                     /locus_tag="AC16FU_00445"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR2992882.1"
                     /translation="MTEPKYVTKLKEKFPGDFVEVNEFRGEWTAVIRTESIVDLCRFL
                     RDDPDCQFNFLSDLCGVDRLNLNQTPRFEVVYHLFSLKFYHRLCLKVSIEETRAVPSV
                     TGVWSAANWHEREVFDMFGLKFTGHPDLRRILMPDDWVGHPLRKDFPLTYEAPKFSHN
                     KDLPPEIVK"
     gene            complement(2235..2762)
                     /locus_tag="AC16FU_00450"
     CDS             complement(2235..2762)
                     /locus_tag="AC16FU_00450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR2992883.1"
                     /translation="MEALQEELKDQVALTTVEKLVNWARKGSLWPLGFGLACCAFEMM
                     SAQASKFDLSRYGMELMRPSPRQADLMIVAGRVSTKMAPVVRQLYDQMPNPKWVLSMG
                     ACASTGGIFNNYAIVQGVDKIVPVDVYAPGCPPRPEQLINAIMVLREKIAREKISGQK
                     EKPIVIGWQQVPVKT"
     gene            complement(2780..3073)
                     /locus_tag="AC16FU_00455"
     CDS             complement(2780..3073)
                     /locus_tag="AC16FU_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR2992884.1"
                     /translation="MIILSAIFSRRTKSLKKFTPYECGVDPVGSARERFPIKYYMVAM
                     LFIIFDIETVFLYPWAVVYKQLGWFGFIEMGIFILLLFLGYVYVLKKGALRWD"
     gene            complement(3416..5713)
                     /locus_tag="AC16FU_00460"
     CDS             complement(3416..5713)
                     /locus_tag="AC16FU_00460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2992885.1"
                     /translation="MASETDILTGKPTLADLLGQLKSDPQFARNIVHWKVLPALPGQY
                     ADFPVRVDQRLRSVLEKRGISRLYSHQAEAMEKIFSGKHMVIVTPPASGKTLCYNLPV
                     LQSILNDPGTRALYLFPTKALSQDQLAELTDLVNAANADIKTYTFDGDTPQDARRAIR
                     QAGHIVVTNPDMLHAGILPHHTKWIKLFENLKYVVIDELHNYRGIFGSHLGNVIRRFK
                     RVCAFYGSKPQFVFCSATIGNPVELAEKILEDKVELVDNNGAPRGEKHFILYNPPVVN
                     AQLGIRRSAVSEAQRLAQRFIGHDVQTIVFAPYRLTVEVLLTGLQEQLRKQGKPDDLV
                     RGYRGGYLPLERREIERGLRDGSIKGVVATNALELGIDIGQLEVAILTGYPGNIASTL
                     QQAGRAGRRQGISVAILVATSSPLDQFLMQKPEYLFSRSPETGNVDPNNLVILTSQVK
                     CAAFELPFEEKEKFGLDATVRLLDHLVEHQVLHKTDGRYHWASEIYPANEVSLRSATP
                     EDFVILNRSDNNRVIGEVDYASAPIFLFPEAIYLHGGEKYQVEELDWEGKKAYVKEAP
                     VDYYTDAETKTELKVLRVANESERSGGQLAHGEVAVTTVPVLFKKIKFFTHENVGNGK
                     ISLPEQTMHTTAFWYVFPENVATITNIPKAQLGSSLRGLANLLQGLVPFWLLCDRRDI
                     RVVPQVKAPFTEKPTLYIYDNVPGGVGFAERLFRIFPGVIQPCFDFLRACGCESGCPA
                     CVGPMLEAEQSGKAGVEKLLLFLKG"
     gene            6063..7037
                     /locus_tag="AC16FU_00465"
     CDS             6063..7037
                     /locus_tag="AC16FU_00465"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HIR2992886.1"
                     /translation="MTRLIKAHGLLEKQPGFYTRNILLTWGLLAVGLIFLAVTDNFWL
                     LLLNAAYLAFVFTQISFLSHDAGHRQIFRAAWQNDIFCLVHTDLLLGMSYGWWVKKHN
                     QHHNAPNQVDHDPDLDIVALALSEDQIQSKHGFLRFVAKYQAYFFFPLLLLTAFAMSA
                     KSVQAVLQKKVKYPRVEALLISLHFALWFGLLCYWLGIRQALLFTLLQRALFGLYLGS
                     VFAPNHKGMLILKENNRLDFLRQQVLTTRNVKAHPVTDFWYGALNYQIEHHLFPSMAR
                     NKLGGAQKIVRTFCREHAIPYYESRVLQSYREILQYLHQVSASVSSNY"
     gene            complement(7027..7554)
                     /locus_tag="AC16FU_00470"
     CDS             complement(7027..7554)
                     /locus_tag="AC16FU_00470"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HIR2992887.1"
                     /translation="MKGKIVVVPMGDFDFYLVNKLATALSTMFAVPVDILQGMKIPQE
                     AYNWERGQYYSTVILSKLELLKANERERVLGVVDEDLYVPALNYVFGESDRLASVAVI
                     SLYRLRQDLFTAGDDDRLVYQRMLKEGVHELGHVYGLSHCNNLKCVMYFSNSMLDTDR
                     KSEKFCDVCLRRLSN"
     gene            complement(7616..8560)
                     /locus_tag="AC16FU_00475"
     CDS             complement(7616..8560)
                     /locus_tag="AC16FU_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR2992888.1"
                     /translation="MRKETSIGIDLGGTAIKSGLVTPTGRVVLELVRKAEVPKGRAHL
                     LGRLRQTITDLLVIAREKKIKVTGIGLGSPGTVNVKTGVVVGNCPNIPHWVGVNLVRE
                     IGRKFRYPFACDNDANLMALAELRLGAARGVKTAICLTLGTGVGGGIVINGEVYRGAW
                     YAGAELGHTTIAFDGPRCKCGNYGCLEVFVSAPALVRRFQQSARGRATFGRITPEKIF
                     ALARGGNREARVAVAETGRMLGVGLASFVNAFNPEVIVIGGGVAEGGPLLLQAAKREL
                     RRRAFVSATRGLQVKRAQLGNRAGFIGAALYVGEAQNR"
     gene            complement(8616..9845)
                     /locus_tag="AC16FU_00480"
     CDS             complement(8616..9845)
                     /locus_tag="AC16FU_00480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2992889.1"
                     /translation="MDQAAVKEPAQARVPALSPRARVMPPSPIRKLVPLADQAKKKGV
                     KVYHLNIGQPDIPTPEPFLAAVRSFDKKVLEYSNSRGDAGLIEALVTYYRAKGLVVTS
                     EEIQVTTGGSEAILFALLCVTSPGDEVLVFEPFYTNYNGFAVMAGVTLIPVPTSPETG
                     YHLPTREKIEAKISPRMKAILICTPNNPTGTLYTRAEMQLLADLTQEHNLFFISDETY
                     REFAYDGRHVSILEFPGLDGQAILVDSISKRFSACGARVGCLVSRNRQIVNAAWRFGQ
                     ARLSSPTIEMQAAIPLMQLDQNYYDALVSEFKKRRDTVVHLLTKIPGAFCVPPEGAFY
                     IMATLPVDDTEEFARFLLADFSHQGSTTMIAPGSGFYATPGQGKTEARIAYVLNTGDL
                     EKAMQILAKAAEAYQLK"
     gene            9942..10340
                     /locus_tag="AC16FU_00485"
     CDS             9942..10340
                     /locus_tag="AC16FU_00485"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2992890.1"
                     /translation="MSIKRKSKTRLPTPEEARKLGAIFQSLGDLTRQKILFLLERGPR
                     SVSELVKPFSLTQPTVSRHLAVLKHAGLVTSQRRGQKVIYRLNPEVLRFCHDGFFCCF
                     DCCPVVPLARVMRTAGRGRVTRPIGRGQVA"
     gene            10388..11188
                     /gene="arsM"
                     /locus_tag="AC16FU_00490"
     CDS             10388..11188
                     /gene="arsM"
                     /locus_tag="AC16FU_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR2992891.1"
                     /translation="MNKEETREVVREAYGKIARSQQSCGCGPCGPDATEFAESIGYSE
                     EELKTIPAEANLALSCGNPTALANLKNGEVVLDLGSGAGFDCFVAASKVAPDGKVIGV
                     DMTPEMVEKARDIVRKNGVENVEFRLGEIESLPVEDNSVDVVISNCVVNLSADKPRVF
                     QEVYRVLKPGGKIAISDIALLKELPKKIRESLEAYVGCVGGAILIDEYKKIVEASGLK
                     EVRISIKGSSACIDPTTKDPMGKAILDSLEESESLEGYVVSVYVEGYK"
     gene            11611..12402
                     /locus_tag="AC16FU_00495"
     CDS             11611..12402
                     /locus_tag="AC16FU_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR2992892.1"
                     /translation="MGLKIFQVDAFTDSPFTGNPAAVCLLPGARDEIWMQNVAQEMNL
                     SETAFLYKEGDFFNLRWFTPVVEVDLCGHATLASAHVLWETGLLSPQEPAKFQTRSGF
                     LTAERKRDGIELNFPATPDEPATAPPELSRALGVKPKYIGKTKFDYLVEVGTEGEVRN
                     LKPDFHLLKTLPVRGVMVTSKATSPGYDFVSRFFGPGSGIDEDPVTGSSHCCLGPFWA
                     KRIGKNDLVAYQASPRGGTLRVRVAGDRVYLLGQAVTIFKGELMA"
     gene            12463..13260
                     /locus_tag="AC16FU_00500"
     CDS             12463..13260
                     /locus_tag="AC16FU_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992893.1"
                     /translation="MMLREEFTRQTAQSVRQKTMMLFLIAVLIVLPLTQPVSQDSKTD
                     MWYENTAAAKKNMGHPRSKTSDSGLRIFKRAKSFGDWLSPSEFKFSTSHYNFNITGGG
                     RGTRKLGISDSATFQLKLDTLMCGGETYIRSIYYLESEADLILIYEMSDDESGWGSVT
                     RLNQERLSPEWTEHVPSFGIKGVVESSYVYFMASGYVAKLNLDSGKYVWQHKNLYKDY
                     HFNNFELPIIEGDVVLFKEIPQDLPKQKLRAKTIKVQKQSGKILSIH"
     gene            complement(13425..14459)
                     /locus_tag="AC16FU_00505"
     CDS             complement(13425..14459)
                     /locus_tag="AC16FU_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2992894.1"
                     /translation="MDLTSVKRILVIRLSSFGDLVLTQPVFANLKAACPDASVELLVR
                     EKYAPLFEPGPCTGGADVVHTFEQFRPSQEIFGLLFDLHGNWRSRQVRRQIRAGRVFV
                     YPKQRVARMARVWLKRFSPFRLRPTLELYFDVLRQAGIPILTSEPNWRVHESARQEAG
                     KYLTNQKAGQGDFLVGLGPGARWETKRWSAERFIETGRLLNRARPARFVLFGSKGEEQ
                     LLRQIAAELPDPKPLIISDAPLGLLPALLAELRLFISNDSGLMHLAVAVGTPTIGIFG
                     PTHPALGFAPTGKHTRTLHHPVWCSPCSLHGSKPCFRSRRFCLEGITPEQVVRVADAL
                     LNQKVATVLR"
     gene            complement(14460..15281)
                     /locus_tag="AC16FU_00510"
     CDS             complement(14460..15281)
                     /locus_tag="AC16FU_00510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR2992895.1"
                     /translation="MNFGQAIILGILQGLTEFLPISSSGHLVITERLFGLKLANIVFD
                     VAVHVGTLVAVLVYYRKKLLTILRAMLSSTGFGSMASVPAGVSEKADARKLFWYLVAG
                     SLPAAVIGLSFKGFFESTFTEPRLVSAFLMVTGLVLFSTRFARAGSQRLNLWRALAVG
                     CAQAVAILPGISRSGSTIAAALLLGVAGPDAAEFSFLLSVPAVAGAAFLELRHLNGVG
                     ADSQLLFLCVAGALAAFAVGLGAIHFLVSYLKRGNLVPFGYYCLAVGGFFVYYFR"
     gene            complement(15371..16015)
                     /locus_tag="AC16FU_00515"
     CDS             complement(15371..16015)
                     /locus_tag="AC16FU_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992896.1"
                     /translation="MKRVGILALVGLTVLGAATLAPAQQMISTMNSARVLAPGASMAG
                     GYFGVFDGGISVYGQYRYGFSLPFEGGLQFGFVDFDGDGNSGVILGGDGQFQIIDAKL
                     HDPFDLSAGGLVQFAFVGDLNIFSLGGSMAISKDLPLRSGRSFTPYGRLQLRMDRADV
                     SVNTPFGRISGSNTDFNVAFNIGGDLEIANNTDLVSEVQLADNVGFLIGFNVHF"
     gene            16353..16823
                     /locus_tag="AC16FU_00520"
     CDS             16353..16823
                     /locus_tag="AC16FU_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992897.1"
                     /translation="MDYGTTSEMHRELLILLRDELSFYQSLYILIDRQRDLLKHDREE
                     ELAKVFAEIDRYTQRIKESELKLRALARKHRDVLAVVAASEEIRRLVQSIAMLIEKNL
                     DTAKANEAFIAQRHAKIQDQLDELEDSHDTLKLLRGTTGKVPVKTTPSVSNRER"
BASE COUNT         3975 a         5069 c         4776 g         3710 t
ORIGIN      
        1 gtccggtgtc gtctccccgt ttttgatgcc taacttccgc tcgaggtaag aaacgagact
       61 atccgctccg cagagcgcgc aggaaatgtt cgtgcagacc atgatcaggt atttgccgac
      121 ttttttcttg gggaaaagcg tgtagaacgt cgccgactcg gcgatttcca ccgccgggat
      181 gccggtcacc tgaccgatct cggcgtaaac ttcgtcattc aggtagccga gttggtcgta
      241 agcggcgtgg agggccggga gaatcgccga acgccgcacc ggaaatttcg gcagcgcctg
      301 ccgcattttt tgaatcgtct gcggcaagag aagtttgatt ccgggcgaag ggcccgtctt
      361 tcccggcttt ttttcaggcg tcgcgacagc catcagcggt caatctcccc caagacgatg
      421 tcgatgcttc cgatcgtggc caccacgtca gccaccaggc ggccttcgac taatttggat
      481 aaggcgctca aattcacgaa cgacggcgcg cgcacgtgca tccggtaggg cttcgggctc
      541 ccgtcggaga cgaagtagaa tcccagttct cctttgggtg attcgatggc ctggtagact
      601 tctcccaccg gcggtcggaa tccttcggtc cagtacttga actggaagat caaggcttcc
      661 atgctgtaga gcacttcccg cttgggcggc ggggtgaatc ccggcatccg ggacattacg
      721 tcaccctccg gcatcccatc caacgcctgc cggataatcc gcaaactctg tcgcatttcg
      781 gcgatgcgca ccagataccg gtcgtagaca tcgccgttct tgccggtcgg gatttcaaaa
      841 tcgaacttct cgtagccgga gtaggggttc gacttgcgga tgtcccactt cacgccgcag
      901 ccgcgcaggc tggggccgga gaggccggcg tcgatcgcat ctttcgccgc aatgacacca
      961 acgccaatcg tccgattcct ccagatgggg ttgttcgtca ggagattttc gtactcgtca
     1021 aaccgcttcg gaaaatctcg gaggatggcg cggatcttcg gctcaaagtc gggcggaacg
     1081 tcctgcacca cgccgcccac ccgaaagtag gtcgacatca tccgctggcc ggagactaat
     1141 tcgtagattt caatgacccg ctcccgctcg cggaaagcgt acaaaaacat gctcatggcg
     1201 ccgatgtcga tggcgtgcgt gccgatccag agtaagtggc tcttgatgcg cgtcagttcc
     1261 gtcaaaagca cccgcaggta ctgcgccttg ggcggaatct ccatcccgat caacttctcc
     1321 accgccagac agaaccccaa gttgttggac atcggcgccg cgtagtccat ccggtcggtc
     1381 atcggcaggg cggtgtagta cttcttgtcc tcggcgttct tttcgatgcc ggtgtgcagg
     1441 tagccgatct caggcgtcgc tttgatcacc gtttcaccgt ccagctccag aatgacccgc
     1501 aggaccccgt gcgtggaggg gtgctggggc cccatgttga gcgtcatcgt ttcggtttta
     1561 agcatagtgg aggcgaatca gttttggctt gtaccttttg tcacaaacga gcgcgcaatt
     1621 tatatgtccg gcaattttcc aacaaggtta tttcacgatt tcaggcggta agtctttgtt
     1681 gtgagaaaac tttggcgctt cgtaggtcaa gggaaagtct ttccgcaaag ggtgccccac
     1741 ccagtcgtcg ggcatcaaga tgcgccgcag gtcggggtgg ccggtgaatt tgaggccgaa
     1801 catatcgaag acttcccgtt cgtgccagtt cgccgccgac cagacccccg tgaccgaagg
     1861 cacagcccgg gtttcttcga tgctcacttt caagcagagc cggtggtaga acttcagtga
     1921 gaagaggtgg taaacgacct caaagcgcgg cgtctggttt aagttgagtc gatcgacccc
     1981 gcagaggtcg gagaggaaat tgaactggca gtccgggtcg tcacgaagga agcgacagag
     2041 atccacgatg ctctcggtgc ggatgaccgc cgtccactcc ccccggaatt cattgacttc
     2101 gacaaaatcg ccgggaaact tttcttttaa ttttgtaacg tatttcggtt cagtcatctc
     2161 ttcctatgtt gcaagttgac gccgggacgg ctacaaaatc cgttccctac cacctttgac
     2221 ctgccaccta tcacctatgt tttaaccggc acctgctgcc agccgatcac aatcggtttc
     2281 tctttctggc cggaaatttt ctcgcgggcg atcttctccc gcagcaccat aatggcattg
     2341 atcaactgct ccggccgcgg ggggcacccc ggggcgtaga cgtccaccgg cacgatttta
     2401 tcgacgccct ggacgatggc atagttgttg aaaatcccgc ccgttgaggc acaggcgccc
     2461 atggagagca cccacttggg gttgggcatc tggtcgtaca actgccgcac caccggcgcc
     2521 atcttggtgg aaacgcgtcc cgccacgatc atcaagtcgg cctggcgggg ggaggggcgc
     2581 atcagttcca tcccgtagcg ggataagtcg aacttggagg cctgggcgct catcatctca
     2641 aaggcgcagc aggccagccc aaaacccagc ggccagagcg acccttttcg cgcccagttg
     2701 acgagtttct ccactgtggt cagcgccacc tggtctttca gttcttcctg gagcgcctct
     2761 atcgccttct ctttgacatc taatcccacc gcaacgctcc tttcttgagg acgtagacgt
     2821 atcccaagaa aagcaggagg atgaaaatcc ccatctcgat gaagccgaac cagcctaact
     2881 gcttgtacac caccgcccag gggtacagga agaccgtctc gatatcgaag atgataaaaa
     2941 gcatcgccac catgtagtac ttgatcggaa agcgctcccg ggccgagccg accgggtcca
     3001 ccccgcactc gtagggcgta aactttttca aactcttagt ccggcggctg aagatggccg
     3061 agagaatgat catcgtcgcc ccgatgccgg tggccaggat gaccataacg aatataggga
     3121 agtaggtttt catttacccc tgatacgtca tctatgtgaa tattttcaca aattctttca
     3181 taccaaaaac atcttgtact ctttgaatat aatacgaatt ttcaatctgt tctgtcaacc
     3241 cgtaattctt gacaagaaca gtcggctatt tatgtcgttt ctttccccct gatcattttc
     3301 atggcttagc gttcacgttc aaatgttatg gcttcggtct gacctatgga ccgaaagcgc
     3361 cttccacccc atagccgagg cttttagcct cggaaagcag gttctcaaca atcctttatc
     3421 ccttgaggaa taaaagcaac ttctccactc ccgcctttcc cgattgctct gcttcgagca
     3481 ttggccccac gcaggcggga cagccgcttt cacagccaca ggcgcgcaag aaatcgaaac
     3541 agggttggat cactccgggg aaaatgcgga agagccgttc ggcgaaaccg acccctccgg
     3601 gtacgttgtc gtagatgtag agcgtcggtt tttcggtgaa cggcgctttc acctgcggga
     3661 caacgcgaat atcccgccga tcgcacaaaa gccagaacgg caccaacccc tggagcaggt
     3721 tggccagccc gcgtagactg gaacctaact gcgctttcgg aatattggtg atggtagcga
     3781 cgttctccgg gaagacgtac cagaaagcgg tggtgtgcat cgtctgctct ggcaaagaaa
     3841 ttttcccgtt gcccacgttt tcatgagtga aaaatttgat ctttttgaat agtaccggaa
     3901 cggtagttac cgcaacttcg ccatgagcca gttgcccgcc agagcgttcg ctctcgttcg
     3961 ccacccgcag gactttcagt tcagtcttgg tctccgcatc ggtgtagtaa tccaccggcg
     4021 cttctttcac gtacgccttc ttcccttccc agtccaattc ctccacctgg tacttctccc
     4081 cgccgtgcag gtagatcgct tcgggaaaga ggaaaatcgg cgcgcttgcg taatcgacct
     4141 cgccgatgac gcggttgtta tccgagcggt tcaggatgac gaaatcctcc ggcgtagccg
     4201 accgcaggga gacttcattg gccgggtaaa tctccgacgc ccagtggtag cggccgtcgg
     4261 tcttgtgcag cacctgatgc tccaccaaat ggtcgagcag acgaaccgtg gcgtctaaac
     4321 cgaatttttc tttctcttcg aacggcagct cgaacgccgc gcacttcacc tggctcgtca
     4381 ggatgacgag gttattcgga tcgacgttcc ccgtctcggg cgaccgggag aaaaggtact
     4441 ccggtttttg catcaggaac tgatctaagg gcgaactggt ggcgactaaa atcgcgacgg
     4501 agattccctg tctgcgtccc gcccgccccg cctgctgcag ggtcgaggcg atgttgccgg
     4561 ggtagcccgt gagaatggcg acttccaact gcccgatgtc aatccctaat tctaacgcat
     4621 tagttgccac aaccccttta atggaaccgt cgcgcaagcc gcgctctatc tccctgcgct
     4681 caagagggag gtagccgccg cgatacccgc gcacgaggtc atccggtttg ccctgcttcc
     4741 gcaactgctc ctgcaggccg gtgagcagga cttccactgt gagccggtat ggcgcaaaaa
     4801 cgatggtttg aacgtcgtgg ccgataaagc gctgcgccag ccgctgcgcc tcactcaccg
     4861 ccgaacggcg aatccccaac tgcgcgttca ccaccggcgg attgtagaga atgaaatgct
     4921 tttccccgcg cggcgcgcca ttgttatcaa ctaattcgac tttgtcttcc agaatttttt
     4981 ccgctaactc caccggattg ccgatggtgg ccgagcagaa gacaaactgc ggctttgaac
     5041 cgtagaaggc gcaaacccgc ttgaaccggc ggatcacgtt ccccaagtgg ctgccgaaga
     5101 tgccgcgata gttatgcagt tcgtcgatga cgacgtactt gagattctcg aacaatttga
     5161 tccacttggt atggtgcggc agaatgcctg cgtggagcat atccgggttc gtgaccacta
     5221 tgtgccccgc ctgccggatc gcccgccgtg catcctgcgg cgtgtcgccg tcaaaggtgt
     5281 aggttttgat gtcggcattc gccgcgttga ccaaatcggt taactcggcc agctgatcct
     5341 gcgacagcgc cttggtggga aaaagataga gcgcccgcgt gccgggatcg ttcaggatag
     5401 actgcagaac cggtaaattg tagcagagcg ttttgcccga agcgggcggg gtgacgatca
     5461 ccatgtgttt gccggaaaaa attttctcca tcgcctcggc ttgatgagaa tacagccgcg
     5521 agatcccgcg tttttccaga accgaacgca aacgctgatc cacccgcacc gggaagtcgg
     5581 catactggcc gggcagagct ggcaaaacct tccagtggac gatgttcctg gcgaactgcg
     5641 ggtcggattt cagttgcccg agcaggtcag ccagagtcgg ttttccggtc aggatgtcgg
     5701 tttcggatgc catagtatga acctggtccg ccttaagaac agtcttaact tagcgtgccg
     5761 acaagaaaat acccaaaccg tcaaattgca atcgtaattt ttcccttgac tgagggcaat
     5821 gacgtcccct attttttgaa caagacacct ttgatgtgaa aaaaagtgat ggaaggagaa
     5881 atggaattct cgctttgaag tcacggagcg aatcgacacg gaggctgact tgcttaaaca
     5941 aaaaaaagaa tcaggcgtcg ttgctgagta gagatcgtgg agttgaggta acgggcggta
     6001 aaaggcctca tctttattcc ccggtgtcgc cacaacactc accgacaaat gaatatggag
     6061 aattgacccg gctcatcaaa gcgcatgggc tactggagaa acagccgggc ttctataccc
     6121 gcaatatctt gctcacgtgg ggcctgctgg ctgtggggct catcttcctc gcggtcaccg
     6181 acaacttctg gctgctattg ctcaatgccg cttacctcgc ctttgtcttc acgcagataa
     6241 gttttctgag ccacgacgcc ggtcaccgtc agattttccg cgctgcctgg cagaacgata
     6301 ttttctgcct cgtccacacc gacctgttac tcggaatgag ctacggctgg tgggtcaaga
     6361 aacacaatca gcaccataac gctcccaacc aggtcgatca tgatcccgat ctcgacatcg
     6421 ttgctctggc tctctccgaa gaccagatcc agagcaagca cggttttcta agattcgttg
     6481 ccaaatatca ggcctatttt ttctttcccc tgctgttgtt aacggccttc gccatgagtg
     6541 ccaagagtgt gcaagccgtt ttgcagaaga aagtaaagta cccccgggtc gaagcgctcc
     6601 tcatcagcct ccactttgca ctttggttcg ggctgctctg ctactggctg gggatccggc
     6661 aagcgctgct gttcacgctc ctgcagcggg cgcttttcgg gctgtatctg ggctcggtct
     6721 tcgcccccaa ccacaaaggc atgctcatcc tgaaggagaa taaccggctg gacttcctgc
     6781 ggcagcaggt tctcacgacg cgaaacgtaa aagcccatcc ggtcaccgat ttctggtacg
     6841 gcgcactcaa ttaccagatc gagcaccacc tgttcccgag tatggcccgg aacaaattgg
     6901 gcggggcgca gaaaatagtc aggaccttct gccgggagca tgccataccc tattacgaat
     6961 cccgggtgct gcagtcgtac cgtgaaatcc tgcaatatct gcaccaagtg agcgcttcgg
     7021 tttcttctaa ttactgagcc tgcgcaggca aacgtcgcag aatttctccg acttccggtc
     7081 ggtgtcgagc atcgagttgg agaagtacat cacgcacttg aggttgttgc agtgcgagag
     7141 tccatagacg tggcccagct cgtgcacccc ttccttgagc atccgctggt agaccaagcg
     7201 gtcgtcgtcg ccggcggtaa agaggtcctg ccgcaggcgg tagagcgaaa tcaccgccac
     7261 cgacgccaag cggtccgact cgccgaaaac gtagttgagc gccgggacgt agaggtcttc
     7321 atccaccacg cccaggactc tctcccgctc gttggctttg agcagttcga gtttgctcaa
     7381 aatgacggtc gagtagtact gcccccgctc ccagttgtag gcctcttgag gaattttcat
     7441 cccctgcagg atgtccaccg gcaccgcgaa catggtggag agcgccgtcg ccagcttgtt
     7501 gacgaggtag aagtcgaaat cccccatcgg aacgacgacg attttgcctt tcatgcgtga
     7561 gcgtgcctcg aatagtcgct atcaaacgaa agcataaccg ggggaggtag ggctgtcaac
     7621 gattttgcgc ttcgccgacg tacaaagcgg cgccgatgaa gccggcccgg ttgcccagct
     7681 gcgctcgttt tacctgaagg ccgcgcgtgg ccgaaacaaa cgccctccgg cgcagttccc
     7741 ttttcgccgc ctgcagaagg agaggacccc cttccgccac gccgccgccg attacgatca
     7801 cttccggatt gaaggcattg acaaaagaag ccagcccgac acctaacatc cgtccggttt
     7861 cggcaaccgc tacccttgcc tcccgatttc ctccgcgcgc cagcgcaaaa atcttttccg
     7921 gagttatgcg gccaaacgtg gcccgacccc gcgctgactg ctgaaaacgc cggactaacg
     7981 ccggtgctga aacgaagacc tccagacaac cgtaattccc gcacttgcag cgggggccat
     8041 cgaaagcaat cgtcgtatgc cccaactcgg ctccggcata ccacgctccg cggtaaacct
     8101 ccccgttgat gacaatccct ccccctacgc cggtgcccag cgtgaggcaa atcgcggtct
     8161 tgaccccgcg cgcggcaccc agacgaagtt cggccaaagc catcaaattc gcatcattgt
     8221 cgcaggcgaa cgggtagcga aattttcggc caatctcccg caccagattc acccccaccc
     8281 agtggggaat attgggacag ttgcccacga ccacgccggt tttgacattc accgtccccg
     8341 gactccccag gccgatgccg gtaaccttaa tttttttctc tcgcgcaatt accaggagat
     8401 cggtgatagt ctggcgcagc ctgcccaaga ggtgcgcccg gcctttgggc acttcggcct
     8461 tgcgaaccag ttcgagcacg actctccccg tcggcgtcac cagtccgctc ttgatggccg
     8521 tgccgccgag gtcaatgccg atggaagttt ctttgcgcat tagagataaa aaaacggtcg
     8581 ggaaagcacc gaccgttacg ccggtgaacc ctgtcctatt tcaactgata cgcctcggcc
     8641 gccttcgcca gaatctgcat cgctttctct aaatcgccgg tattgaggac ataggcgatg
     8701 cgcgcttcag tctttccctg gcccggggtg gcgtagaaac cggacccggg cgcaatcatc
     8761 gtcgtgctgc cctgatgcga gaaatcggcc agaaggaagc gggcgaactc ttcggtgtca
     8821 tccaccggta gcgtggccat aatgtagaag gccccttccg gcggaacgca aaaagctccg
     8881 ggaatcttcg tgagcagatg taccaccgtg tcccgccgct tcttgaattc actgacgagc
     8941 gcgtcgtagt agttttggtc caactgcatc aatggaatag ccgcctgcat ctcgattgtg
     9001 ggcgaagaga gccgcgcctg accgaagcgc caggccgcat tgacgatctg ccggttgcgg
     9061 gaaaccaggc agccgaccct ggcgccgcac gccgaaaaac gtttggagat gctgtctact
     9121 aaaattgcct gaccgtcgag tccggggaac tctaaaatcg aaacgtggcg cccgtcgtag
     9181 gcgaactcgc ggtaggtctc gtccgaaatg aaaaagagat tgtgctcctg cgttagatcg
     9241 gccaaaagct gcatttccgc ccgcgtgtag agcgtgccgg tgggattatt gggcgtgcag
     9301 atgagaatgg ccttcatgcg gggagagatt ttcgcttcga tcttctcccg cgtcggcagg
     9361 tggtagcccg tctccggtga cgtgggcacg gggatcaggg tgacccccgc cattaccgca
     9421 aagccgttat agttggtgta gaacggctcg aagaccaaaa cttcatcgcc gggtgatgtg
     9481 acacacaaaa gcgcaaacag gatcgcctcg ctcccgccgg tggtgacctg gatttcttcg
     9541 ctcgtcacca ctaacccttt cgctcggtag taggtcacga gggcctcgat caaccctgca
     9601 tccccccggg agttgctgta ctctaaaacc tttttgtcaa aactgcggac ggcggccagg
     9661 aacggctccg gggtggggat atccggctgt ccgatattga ggtgatagac tttaaccccc
     9721 ttctttttgg cctggtcggc taaggggacg agtttgcgga tgggcgaagg cggcatcacc
     9781 cgcgcgcggg gcgagagcgc cgggacgcgt gcttgggctg gctctttgac ggctgcctga
     9841 tccacggaag cagtataggg agggtgccgc tgccttgtca aaccaatttt ccaccaacgg
     9901 cggcgtttcg ctgccgtatt aatatataga atggtttgca tatgtctata aaacgaaaaa
     9961 gcaaaacgcg cctgcccacg ccggaagaag cccgcaaatt gggagccatc ttccagtcct
    10021 tgggtgatct cacccggcag aagatcctgt ttttgctgga acgcgggccc aggtcggtca
    10081 gcgaactggt caagcctttc tccctcaccc aaccgaccgt gtcgcggcac ctggcggtgc
    10141 tcaagcacgc cggcctggtg acgagtcaaa ggcgcggcca gaaggtcatc taccggctca
    10201 atccggaagt cctgcgcttc tgccacgacg gcttcttctg ctgcttcgac tgctgtccgg
    10261 tcgtgccctt ggcgcgggtg atgcgcacag ccggcagggg tcgggtcacg cgacccatag
    10321 gcaggggtca ggtcgcctga ccccagacga gcagagttgc tatcaggcaa aaacaggagc
    10381 tgtcaaaatg aacaaagaag aaacacggga agtcgtgaga gaagcttatg gcaagatcgc
    10441 tcgaagtcaa caaagctgtg gctgcgggcc ttgtggacca gatgccacag aattcgccga
    10501 atctattgga tattcggaag aagaactgaa aaccatccca gctgaggcca atctggctct
    10561 tagctgcgga aatccgaccg ccttagccaa tctcaagaac ggtgaggtgg tacttgacct
    10621 tggttccggt gccggttttg attgctttgt agctgcatcg aaggtagctc cggatggtaa
    10681 agtcatcggt gtggacatga caccggagat ggttgagaaa gcaagagaca ttgtcagaaa
    10741 gaacggagtt gaaaatgttg aattcaggct tggcgagatt gagagcttac ccgtggaaga
    10801 caattcggtg gatgtggtca taagcaactg cgtggtcaac ctctcagccg ataagccgag
    10861 agtcttccaa gaggtctata gggtcttaaa gccagggggg aaaatagcga tttctgatat
    10921 tgcccttctc aaggaacttc ccaagaagat tcgggagagc ctggaagctt atgtgggttg
    10981 tgtaggaggg gcaatactta tcgacgagta caaaaagatc gttgaggcgt ctggtttgaa
    11041 ggaggtaaga atttcgatca aaggctcttc tgcctgtatc gatcccacga ccaaagaccc
    11101 aatgggtaag gccattctag atagcttgga agagagtgag tctctcgaag gctatgtggt
    11161 tagcgtctat gtggagggtt ataagtaagt ggcagcaatt ttttgcctaa ccattcgttc
    11221 cggcggacgt ggtaaaccgc gccccccaac tttgtcgatc gaacgacccg aatgcctgac
    11281 ccgcaaaagg aggtgatacg gatgctgcgt cgccgctgga tgctccgcgc ggtctgcaac
    11341 tgcccctgcg gttgccatag ccggtcctga gcacgacgct aaactgctcg accaaccccg
    11401 gatcggtgtg aatgtccgat cccgatctcc gaccggtcga atttcaaccc cgccctcttt
    11461 ggcggggttt tttatggtac accctttaat caaaactgcg tagaagcatc tctggtcgta
    11521 gccgccctaa atgaaccccg aacccctttg acacgcgaca aggccgtaga atagtttccc
    11581 gtaccggtag ttcagaaacg ggaggaacag atgggattga aaatttttca ggttgacgct
    11641 ttcaccgaca gtcccttcac cggcaatccg gcagcggtct gccttctgcc gggagcgcgc
    11701 gatgagattt ggatgcagaa cgtcgcgcag gagatgaacc tgtcggagac tgccttccta
    11761 tataaagaag gagacttttt caacttgcgc tggttcaccc ctgtggtcga agttgacttg
    11821 tgcggccacg ccaccctcgc cagcgcccac gtcctctggg agactggcct gctctcccct
    11881 caggaaccag cgaaatttca aacccggagt ggattcttga ccgctgagcg caagcgcgac
    11941 ggtatcgaat taaatttccc ggccacgccg gacgaacctg cgacagctcc tccggaactg
    12001 agccgggccc tgggggtgaa gccgaaatac atcgggaaga ccaaattcga ttacctcgtg
    12061 gaagtgggga cggagggaga ggtacggaac ttgaaacctg attttcacct cttgaaaacc
    12121 ctgccggtgc ggggcgttat ggtcacgagt aaagcaactt ccccgggata tgattttgtc
    12181 tcccgcttct ttggcccggg ctcaggcata gacgaagacc ccgtcaccgg ttcctcccac
    12241 tgctgtctgg gccccttctg ggccaaacgg atcggcaaga acgaccttgt tgcctaccag
    12301 gcctcccccc gcggcggaac tctgcgcgtt cgcgtcgccg gagaccgcgt ctaccttctc
    12361 ggccaggcgg ttaccatttt caaaggcgaa ctaatggcct gatgagagaa cgaacgaaag
    12421 aggtcttgct gaaaattatg cgataatttc cgataactac taatgatgct ccgcgaagaa
    12481 ttcacacgtc agaccgccca atcggtgaga caaaagacca tgatgttatt cctgatcgcg
    12541 gtcctgattg tcttacccct aactcaaccc gtctcgcaag actcaaagac tgatatgtgg
    12601 tatgaaaaca ccgctgcggc caagaagaat atggggcatc cccggagcaa aacctctgat
    12661 tctggtctca gaattttcaa aagagcaaaa tctttcggag attggctctc cccctctgaa
    12721 ttcaaattct ccacttccca ttacaatttt aatatcaccg ggggtggtcg tggcactcgc
    12781 aagctcggga tatccgattc agccactttc caactaaaat tggacacgct tatgtgtggt
    12841 ggtgaaactt atattcgaag tatttattat ctcgagtcag aagctgactt gatattgatt
    12901 tacgaaatgt ctgatgatga atctgggtgg ggttccgtca cccgattaaa tcaggaacgc
    12961 ctgtcaccgg agtggacgga acacgtacct agtttcggca ttaaaggtgt tgtagagagc
    13021 agctatgtat acttcatggc atcaggctat gtagccaaat tgaatcttga ctcagggaaa
    13081 tacgtctggc aacataagaa tctgtacaag gactatcatt tcaataactt tgaattgccc
    13141 ataattgaag gcgacgtggt cttattcaaa gagatccctc aagacctccc caaacaaaaa
    13201 ttgagagcca agaccattaa agttcaaaag caaagcggca aaattctgtc tatacattag
    13261 cacgtagccg aggtttccaa cctcggcgat ccttatggat cgtcgccttt gaactgtcag
    13321 acctaaacga tgccgaaaat cgtcgctgac gcactcttac gttaggaatt caccctctcc
    13381 tcgtaggaaa gggaggtcga agacgcgcct caggcggggt gaggtcaacg caaaacagtc
    13441 gcaacttttt ggttcaataa cgcatcagcc actcgtacca cctgctccgg cgtaattccc
    13501 tccaagcaaa acctgcgtga tcgaaagcac ggcttgctcc cgtgtaaact acagggcgag
    13561 caccagacgg gatgatgaag cgtgcgcgtg tgtttgcccg tgggagcgaa tcccagggcc
    13621 ggatgcgtgg ggccgaagat gccgatggtg ggtgtcccca ccgcgacagc gaggtgcatt
    13681 aaaccgctgt cattggaaat gaaaaggcgt aattctgcca gaagcgctgg gaggagaccc
    13741 agcggtgcat ctgaaataat cagtggtttt ggatcgggca gctcggctgc gatctgacgc
    13801 aatagttgct cttccccctt actgccaaac aaaacaaacc gtgctggtcg agcccggttg
    13861 agtaaccgac cagtctcgat aaacctttcc gccgaccagc gtttggtctc ccaccgcgcg
    13921 ccggggccga gaccgaccag aaaatcgccc tgtccagctt tctgattcgt caaatactta
    13981 cctgcctctt gacgagcaga ttcatgcacg cgccaattcg gttcagaggt cagaatcgga
    14041 atgcccgcct gccgcagaac atcgaagtac aactccagcg ttggccgcag ccgaaaagga
    14101 gaaaaccgct tgagccagac ccgcgccatt cgcgccaccc tttgtttggg gtagacaaaa
    14161 acccttccgg cccgaatctg gcggcgcacc tgccgggaac gccaattgcc gtgcaagtcg
    14221 aacagcaggc caaaaatctc ttgagaagga cggaattgct cgaaagtatg aacgacgtca
    14281 gcgccgcccg tacaggggcc gggttcaaag agcggcgcgt acttctcccg caccagcagc
    14341 tcgaccgaag cgtcaggaca agccgctttc agattggcga aaaccggctg agttaaaacg
    14401 aggtcaccga aagaacttaa gcgaatgacc aggattcttt tgacggaagt caggtccact
    14461 cagcggaaat aatacacgaa aaatccgccc accgccaaac agtagtagcc aaacggcact
    14521 aaattccctc gcttgagata ggagacgaga aagtggatgg cgcccagtcc gacggcgaac
    14581 gccgctaggg ccccggcgac acagaggaac aaaagctggg aatctgcgcc cacaccgttg
    14641 aggtggcgca actccaagaa tgccgcgccc gccacagcgg gtaccgagag cagaaaggaa
    14701 aactcggccg catcgggtcc ggccactccc aaaaggagcg ccgctgcaat ggtcgatccg
    14761 gagcgggaga tacccggcag gatcgccacc gcctgggcgc agcccaccgc cagcgcccgc
    14821 cagaggttca gccgctgcga accggcgcgg gcaaagcgcg tggagaaaag gaccaatcca
    14881 gtgaccatca aaaaggcgct aactaaccgt ggctcagtga aggtgctctc aaaaaaacct
    14941 ttgaagctca gaccgatgac cgccgccggc agtgacccag ccacgaggta ccaaaagagt
    15001 tttcgggcat cggccttttc gctcaccccc gccggtacgg acgccatcga gccgaaaccg
    15061 gtactgctca gcattgcgcg caggatggtg agtaacttct tccggtagta gaccaggacc
    15121 gccacaagcg tccccacgtg caccgccacg tcaaagacga tattcgccaa cttcagcccg
    15181 aagaggcgct cggtgatcac caggtggccg gaggaggaga tcggcaggaa ttcggtcaat
    15241 ccctgcagaa tccccaaaat aattgcctgc ccgaagttca tcgaaagaaa acaggctctg
    15301 gtctgccaga gcctgtttca gcggtacgca aggtgaaatt ccagttgaac aagggccgac
    15361 ggcgctccct ttagaagtgg acgttgaatc cgatcagaaa gccgacgttg tcggccaatt
    15421 gcacctcgct caccaggtcg gtgttgttgg caatctccag gtcgccgccg atgttaaacg
    15481 ccacgttgaa gtcggtgtta ctcccgctga tccggccgaa gggagtattt acactgacat
    15541 cggcgcggtc catccgcagc tgcaggcggc cgtacggcgt aaagctccgt ccggagcgca
    15601 ggggcaggtc tttggagatc gccatgctcc ctccgagcga gaagatgttc aaatctccaa
    15661 caaaagcgaa ttgcaccaat ccccctgcag agagatcaaa ggggtcgtgc aacttggcgt
    15721 cgatgatctg gaactgaccg tcgccgccca aaatgacccc gctgttgccg tccccgtcaa
    15781 agtccacaaa gccgaactgc agcccgcctt cgaagggcaa agaaaatccg tagcggtact
    15841 ggccgtacac cgatatgccg ccgtcaaaca ccccgaagta gccgccggcc atagatgcac
    15901 ctggcgccag aacccttgcg ctgttcatag tgcttatcat ttgctgagcc ggtgcaagtg
    15961 ttgctgcacc taagacggtc agcccgacga gtgctaagat tcccacccgt ttcattttcc
    16021 cctcctctct tgccgttttg ctccgtttat cgacccagcc ccttgcctgg ctgcacatac
    16081 tgtagttcgg ctgaatcaga aggaaagtta acctcccgca gagcggctgt caacaacttt
    16141 tttcgcataa ggcatttttt tgcacactaa cagtggctgc aaacaggcgg cggctgtcct
    16201 tcgtctcaag ccatgccgat tcctgccgaa aagagaaatg aaacgatttt tcgctcgatg
    16261 cgccctgccg cccaccggga accagcgcgc atcgtttggt gaagacgtct gtaatcgtct
    16321 cggtacggag cgtaccgacc aaaggagccg gcatggacta cgggaccacg agtgaaatgc
    16381 accgggagct cttgatcctt ttgcgcgatg aactttcttt ctaccagtcg ctgtacattt
    16441 tgattgaccg ccagcgcgac cttctgaagc acgaccggga agaggagctg gccaaggtgt
    16501 tcgccgagat cgaccgttac actcagagga tcaaagagag cgaattgaaa ctccgggccc
    16561 tggcccgcaa gcaccgcgac gtactggcgg tggtggccgc ctccgaggaa atccgccgcc
    16621 tggtacagtc aatcgccatg ctcatcgaga agaatctgga cacggccaaa gccaacgagg
    16681 ccttcatcgc ccaacggcac gccaaaatcc aggaccagct ggatgaactc gaggactcgc
    16741 acgacacgct gaaacttttg cgcggcacaa caggaaaagt gccggtgaaa acgacgccct
    16801 cggtgagcaa ccgggagcgg tagaatggaa gcagggaaaa tcagaggacg aagccgaacg
    16861 cctcgcacac gagtgcggcc gctccctaca gcacaaatga aaaactctcc gaccggaaga
    16921 accgccccct cccgtgccca gcgttgcccc gctggtatgc gcttgttgac gaccgatcga
    16981 cgccggaaac ggctggggaa cttggctggc tcaaagaaaa aatcccctcc tcagtcacag
    17041 aggatacgtc catcatccct cagcgcttgt ctccagcgct gggaaggtat ccgccctcag
    17101 aaagtacgcc gcgcctggcg ccgggtggcg ctgcgcttct accggaagcc cgaagtgatc
    17161 agtcaggtgg tcgagcgcat cttagatgag ttgacccgcg gcggctggaa atcgtccggc
    17221 ctgccgtccc ccgcttctta aactccggct ttcaaccatt tcctccagct ctaaggcgtt
    17281 cgatcctcgc tcgctttgag cagggttgaa gtactctctt gcaaatagcg aaatggaaaa
    17341 actgcaccaa taaaaaaacc ccaccgcccg gcggggtttt tggggagact ttgacgctac
    17401 ttcatcatcg gcaacgaaaa ggtcagcccg ccgttgatgg cgaagaaacc gaagttatcg
    17461 agaccggaga ccaccgagaa gttcccgggg gtgacgctgc cggagaaact gttgtccttg
    17521 gcaaaaacga
//
LOCUS       DBNGZA010000028         3489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2656069, whole genome shotgun sequence.
ACCESSION   DBNGZA010000028 DBNGZA010000000
VERSION     DBNGZA010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2656069"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="AC16FU_00525"
     CDS             complement(<1..351)
                     /locus_tag="AC16FU_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2992898.1"
                     /translation="MRKSFGWVLASLFILGTFSTCAKQGNVIKVGEYGSLTGTTATFG
                     ISTKNGIDMATEELNAGGGLLGKKVQIIVEDDQGKPEEAATAVQKLISQDKVVAVLGE
                     VASTRSMAGGRICQQ"
     gene            532..1275
                     /locus_tag="AC16FU_00530"
     CDS             532..1275
                     /locus_tag="AC16FU_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR2992899.1"
                     /translation="MSTQILLVLLTSVLAGGANVIGGLIVASRAGWTKQLLRYCLAFG
                     AGFILAAAALRMIPISIQARPDAALLVLAGYFFVHLFEHTVSPHFHFGEETHAVEAGL
                     TRQVGFSAVFGLVMHAFFDGVTISSGFAVTPALGLLLFLAILLHKLPEGFSVASLALA
                     SGHSRRWAVGAAAVVGISTVVGGVAASFLSSLMTYALAFSAGVAIYVAATDLIPEVNR
                     EPGIKMSFMVFLGVALFYITERLLEMLGV"
     gene            complement(1371..>3489)
                     /locus_tag="AC16FU_00535"
     CDS             complement(1371..>3489)
                     /locus_tag="AC16FU_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR2992900.1"
                     /translation="GFGFYPRNAQKLVEDAIAAADPNVNFAQYDNDGDGRVEGIFVVH
                     AGIGAEEAGGSGSLIWSHQWSINPRQVDSVFVSDYSMEPEETTGGRLIPIGVFCHEFG
                     HVLGLPDLYDIDYSTNGIGDWSVMAGGSYNGGSRVPAQFDAWCKVTLGWVTPTNYVAN
                     ADSQTLARVEDNPQVFRLWRGGAIGPQYFLVENRQRKLFDRFLPGDGLLIYHIDETVP
                     GNWDENHRLVDIEQADGLRQLNNSGSQGDAADPFPGATNNRTFDDLSNPNSRDYGNQH
                     TQVGVWNVSNSDSLMTANIEVFFSRPALAIASFWLDDTTFGNGDGVIDSAETVVLRLT
                     VTSLMAAASGVSVVAQADNADILFSDRTSSLGTIASLDSVSNSADPIVFSLAPTLPLP
                     RTVYFSLEVATADSLSVIQATVKGTLGRPRVLIVDDDSEKSYESYYTGALDSLREPYG
                     FRTASLVSTADFASYPTAFWFTGDQRLPALAHQSVLKLQTFLDNGGQLFLSGQSLAEG
                     LAAGPDSTFLTNYLHTRYAGNNPITSSLYTVGKPGDPITDSKKVWIMGADGAVNLTSP
                     DYFSPLPQGPAILTYMSTGRAAGVRYGANYRVVFLGFGFEGITNTRTAQGYETREQFL
                     SDVLNWLVTGRPTYLLGDVSDDGVVDLLDLVWLLQYELSGNPAPKPILQVGDVNCDTA
                     YDMQDLVTLLNHVVFGDPLPCAP"
BASE COUNT          678 a         1007 c         1037 g          767 t
ORIGIN      
        1 ctgctggcaa atgcggccgc ccgccatgct ccgggtgctg gccacctccc ccagcaccgc
       61 caccactttg tcctggctga tcaacttctg aactgcagtg gccgcctctt ccggcttccc
      121 ctggtcatcc tccacgatga tctgcacttt cttccccaaa agtccgcctc cggcattgag
      181 ctcttcggtc gccatgtcga tgccgttctt ggtggaaatg ccgaatgtgg cggtggtgcc
      241 ggtgagggag ccgtattcac cgactttgat gacgtttccc tgtttggcgc aagtgctgaa
      301 ggttcccagg atgaaaagag aagccaatac ccaaccaaac gatttccgca tttttcctcc
      361 ctcgttggcc aatgaatgcc gcctacgcat gagtgcccct taatttgtgg ggtttcgacc
      421 gtttgtcaag tcgagataga accgcactga tttcataatt gacaatcctt ttgccggccc
      481 gtaaaatgtt tccgcgatgg gggagaggcg gcacagcggt cgggggtcgg gatgagtacc
      541 caaattcttc ttgttcttct caccagcgtt ttggcgggcg gggccaacgt catcggcgga
      601 ctgatcgtgg cctcccgggc gggctggacg aagcaattgc tccgctactg cttagctttc
      661 ggcgcagggt tcatcttagc cgcagccgcg ctgcggatga ttccgatttc aatccaggcc
      721 cggccggatg cggcgcttct ggttctggct ggctacttct tcgtccacct gttcgagcat
      781 acggtttcgc cgcatttcca cttcggtgaa gaaacccacg cggtggaagc cggtttgacg
      841 cggcaggtcg gcttctctgc ggtcttcggg ctggtcatgc acgccttttt cgacggggtg
      901 accatctctt cgggttttgc cgtcaccccg gctctgggat tgcttttgtt tttggccatc
      961 ctcctgcaca agctgccgga aggattttcg gtggcctccc tggctttggc ctccggccat
     1021 tcccgccgct gggcggtggg cgccgctgcc gtcgtcggta tttcgaccgt cgtaggaggc
     1081 gtcgccgcct cttttctatc cagtcttatg acctacgctc tggctttttc ggcgggcgtg
     1141 gcaatctacg tggcggcaac cgatttgatc cccgaggtga accgcgagcc gggtatcaaa
     1201 atgtcgttta tggtcttcct gggggtggcg ctgttctaca tcaccgaacg gctcctggaa
     1261 atgttagggg tataaaaaat cagaaccgcc ctgaagggcg gttctaagaa atctttgatc
     1321 agacttccct gcgggggcgg ctcaagctgc tcttcctcct cgctctctga ctacggcgcg
     1381 cagggcaggg gatcgccaaa gacaacgtgg ttcaaaagag tcacgagatc ctgcatatcg
     1441 tacgcggtat cgcaattcac gtcgccgacc tggagaatcg gttttggggc tgggtttccc
     1501 gacagctcat actgtaagag ccagaccaga tcgagcaagt ccaccacgcc gtcgtcactc
     1561 acgtcaccca aaagataggt cgggcggcca gtaaccagcc agttcaggac gtcgctcagg
     1621 aactgctcgc gggtctcata tccctgggcg gtgcgcgtgt tggttattcc ctcaaatccg
     1681 aatcccagga aaacgacccg gtaattggcg ccataccgga caccggccgc tcggccggtg
     1741 ctcatgtagg tgagaatggc cggcccctgc ggcaacggag agaaatagtc gggagaagtc
     1801 aggttgacgg caccgtccgc acccatgatc cacacttttt tgctgtcggt gatcggatcg
     1861 ccgggctttc ccaccgtata aagcgaggag gttatgggat tgtttccggc ataacgcgtg
     1921 tggaggtaat tcgtcaggaa agtcgagtcg ggaccggcgg ccagcccttc ggcgaggctc
     1981 tggccggaga ggaaaagttg cccaccgtta tccagaaagg tctggagttt caggacgctc
     2041 tggtgggcca gcgcgggcaa acgctggtcg ccggtgaacc agaacgcggt ggggtaactg
     2101 gcaaaatcag cggtgctgac cagagaggcc gtgcggaagc cgtatggctc tcgcagcgaa
     2161 tcaagggcgc cggtgtaata actttcatac gatttctcgg agtcatcatc gacgatgagc
     2221 acccgcggcc gccccagcgt gcctttgacc gtggcctgga tcacggatag actgtccgcg
     2281 gttgccacct ccaggctgaa gtaaacggtg cgcgggagcg gcagggtggg ggccagagag
     2341 aagacaatcg ggtccgcgct gttggagacg ctatcgagtg acgcgatcgt gcccaaggaa
     2401 ctggtacggt cggaaaacaa aatatccgca ttatcagcct gagccacaac ggacacgccg
     2461 ctggcagcag ccatgagact cgtcaccgtg agccggagga ccaccgtttc agcggaatca
     2521 atgacgccgt cgccgttgcc gaaggtagta tcatcgagcc agaacgaagc gatggccaga
     2581 gcgggacgag agaagaacac ctcgatattg gcggtcatca agctgtcgga gttggaaacg
     2641 ttccacaccc ccacctgcgt gtgttgattc ccgtagtcgc ggctgttggg gttggagagg
     2701 tcgtcgaagg ttcggttatt ggtggccccc gggaagggat cggccgcatc gccctgactt
     2761 ccgctgttgt ttaactgccg aaggccatcg gcctgctcga tgtccaccag gcggtggttt
     2821 tcatcccagt taccggggac ggtttcgtca atgtggtaga tgagtagtcc atcgccgggc
     2881 aagaaccggt caaagagctt tctctgccgg ttttcgacaa ggaagtactg gggccctatt
     2941 gctcctcctc gccaaagacg aaaaacctga gggttatctt ctacccgggc taaggtctgg
     3001 ctgtcggcgt tggcgacgta gttcgtggga gtaacccatc ccaaagtgac tttgcaccag
     3061 gcgtcgaact gggccgggac ccggctgccg ccattgtaac tgccgccggc catcaccgac
     3121 cagtcgccga tgccgttggt ggagtagtcg atgtcgtaca gatcgggcag gcccaggaca
     3181 tggccgaact catggcagaa gacgccaatg gggatcaatc tcccgccggt ggtctcttcc
     3241 ggctccatgg agtagtcgga aacaaagacg ctatcgactt gcctcgggtt gattgaccac
     3301 tggtgcgacc agataagact cccgctaccg ccggcttcct cagccccaat tccagcatga
     3361 accacaaaaa ttccttcgac ccgcccgtca ccgtcattgt cgtattgagc gaaatttacg
     3421 ttaggatccg cagcggcaat cgcatcttct accagtttct gcgcgttacg agggtaaaaa
     3481 ccaaatccg
//
LOCUS       DBNGZA010000029         5190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_959531, whole genome shotgun sequence.
ACCESSION   DBNGZA010000029 DBNGZA010000000
VERSION     DBNGZA010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_959531"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1569
                     /locus_tag="AC16FU_00540"
     CDS             232..1569
                     /locus_tag="AC16FU_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR2992901.1"
                     /translation="MPEYLVQLILIFALILANSFFAGSEIALVSARRSKIRQLAEAGD
                     TNARIAEALQNQPERLLATVQVGMTVVSTLAGVIGGATIVAVLQKELAQVPVTFIQKG
                     SQTIAIAIVVVSISYLTIVVGELVPKYIAYVYSETLATRLARPVHWISRIFSWAIKLL
                     SVSSRLVVRAIGIKSLPESGAVSEEEIKLLLREGQKKGIFEKTEQELIHSVFEFTDTT
                     VRMAMTPRTDIVAIDLAWDQKRLLRAVTEEGYSRYPVYQGSLDNVVGIIHTKDIINLL
                     VDSELIILQDILRKPYFVPDSKKIGELLRDFQRLQVHLAIVLDEFGGTAGLITLEDIL
                     EEIVGEIQDEYDIEAERYVRLPDGSVWVAARLSLSELREILPGELPPGADADTIGGLV
                     TSALGRIPSLSEAVNAGDYRFTIMEKAGHRITRVRVEKIQNTAYRTQNTGENH"
     gene            1625..2260
                     /locus_tag="AC16FU_00545"
     CDS             1625..2260
                     /locus_tag="AC16FU_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2992902.1"
                     /translation="MVSKILEAVGGWIVALISSSGYAGVVIAMTIESACIPLPSEIIM
                     PFAGYLVFTGRFTMLGASVAGAFGCLVGSILAYGIGYYGGRPLAVKYGRYVLLSQHDL
                     DKADRWFARWGNSVIFFSRLLPIVRTFISFPAGIARMPFWRFCIYSFLGSLPWCWALA
                     YLGKKMGENWVAIRQWFHEIDYVIGGLILLGLVLFIYRHLRGAREAVQAPE"
     gene            2384..2857
                     /locus_tag="AC16FU_00550"
     CDS             2384..2857
                     /locus_tag="AC16FU_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039496.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HIR2992903.1"
                     /translation="MVGRIVKKAIQKPLWTCPKCRKKFVTRNLWHSCGNFTVDQFFQG
                     KSPHAKKLFQKFVALAKKCGPIIVNPSKTRISFQARTRFAGVSKASDEGLICGFLLTR
                     RLESPRFTSIEFFPPRYYGHRFQINRMQDLNQEVLGWLKEAYKVGRQEHLQKKSQ"
     gene            2942..>5190
                     /locus_tag="AC16FU_00555"
     CDS             2942..>5190
                     /locus_tag="AC16FU_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2992904.1"
                     /translation="MDKRYKGSVGFAVLFVLLSVAVISAQPAQPTKTPSLPSLKFEKY
                     VLPNGLQVILHQDHSIPVVAVNVWYHVGSKNEKLGRTGFAHLFEHMMFQGSEHQPGEY
                     FAPLEKVGGSANGSTAEDRTDYWENVPANYLEMALWLEADRMGYLLPAMDQKKLDNQR
                     DVVKNERRQGVDNEPYGKAEEMIPSLLYPMGHPYSWSVIGSHEDLTRASLDDVKDFFR
                     NFYTPNNASLCIAGDFDPKTAKELVKKYFGDIPPGPSVERLKNWVPTLDGEKRIVVPD
                     RVSLPRLYLLWQTTPFYTPGDAELDLLGNILSSGKTSRLYKSLVYEKQIAQDVAAYQA
                     SREMGSNFAVVVTAKQGHTLEEIQKAVDQELAAIRTTGVTPAELIQAQNNFEANFMRQ
                     LQNVGSFGGRADLLNQYNTYLGDPGKLQWDLDRYLKATPEGVKQVAEKYLDPKRRVLL
                     TVVPQEDYQPEAVTVDRSTQPPAGPEPTFTPPKVQRAKLSNGMDVLLVEEHKLPLVQV
                     ELVLKSGWAADPKERPGAASLTAELLDEGTTTKNALQISGEVKSLGAQLGTGSFFDAS
                     TASINLLKKHLDKGLSILSDVIMHPTFPAEELERQRQIYLGRILQESKEPFTSGFKVF
                     LRTLYGPGHPYGQPYTGSGTETSIKAIGRSDLTKYYQTYYAPNNAALVVVGDITLAEA
                     LPKLEAAFKEWKPKEVPKVEIPPVKPLTAPKVFIVDKPGAAQSVILMGNFALARKAPD
                     YIPAQVMNNV"
BASE COUNT         1140 a         1480 c         1500 g         1070 t
ORIGIN      
        1 tggtagtcgg gctcgtcccc gagcccgact actcccgctc aaggatgagc gggagtaccg
       61 gtcttccaat aatcttatgg aattttcaaa tagtatgttt tgggttcgcc gcaaaaatcg
      121 tagttaactt cttatctctc aaagtaatag gaaagtctcg ccagccttta atttcccctt
      181 taacaccatt gtttccggcg caccacctgt tagattatcc ctcggaggcc catgccggaa
      241 tatctcgtcc agttaattct aattttcgcc ctgatcctgg ccaatagctt ctttgccggg
      301 tcggagattg ccttggtctc ggccaggcgg agcaagatcc ggcaactggc cgaggcaggc
      361 gatacgaacg ccagaatcgc ggaagcactc cagaaccagc cggagagact cttggccacg
      421 gtgcaggtgg ggatgacggt ggtttcgact ctggcggggg tgatcggggg tgccaccatc
      481 gtggcggtgc tccagaaaga gttggcccag gtgcccgtca ccttcatcca gaaggggagc
      541 cagaccatcg ccattgccat tgtggtagta tcgatttcct acctcaccat cgtggtcggg
      601 gagttggtgc ccaagtatat cgcctacgtc tattccgaaa cgctcgctac ccggctggcc
      661 cggccggtac actggatctc ccgcattttc tcgtgggcga tcaaactgct ctcggtctcc
      721 tcccgcctcg tggtgcgggc catcgggatc aagtcccttc cggaatcagg ggcggtctcc
      781 gaggaagaga tcaaactgct tttgcgcgag gggcagaaga agggaatttt cgaaaagacc
      841 gagcaggagt tgattcactc ggtcttcgag ttcaccgata ccacggtacg gatggcgatg
      901 accccgcgca ccgacatcgt cgccatcgac ctcgcctggg atcagaagcg gctcctgcgc
      961 gccgtcaccg aggaggggta ctcgcgctat ccggtctacc agggatcgct ggacaacgtc
     1021 gtggggatca tccacaccaa ggacatcatc aatcttttag tcgatagcga actcatcatc
     1081 ctgcaggata tattgcgcaa gccgtacttc gtgcccgatt cgaaaaagat cggcgaactc
     1141 ttgcgcgact tccagaggtt gcaggttcac ctcgccatcg tcttggacga attcggcggg
     1201 acggcggggt taatcacgct cgaagacatc ttggaagaaa tcgtgggcga aattcaggac
     1261 gagtacgaca tcgaagcgga gcgctacgtg cgcctgcccg acggctcggt gtgggtggcg
     1321 gcgcggcttt cgctctccga actgcgcgag attcttcccg gagagttgcc gccgggagcc
     1381 gatgccgaca ccatcggcgg gctcgtcaca agcgccttag gccggattcc atcgctctcc
     1441 gaggcggtga acgccgggga ttatcgtttt accatcatgg agaaagcggg gcaccggatt
     1501 acacgggtgc gggtggagaa aattcagaac acggcatacc ggacacagaa tacaggggag
     1561 aatcattgac ccaacttctc attcccagtt cccaacaaat ccgaaagtct ggcgttcccc
     1621 ggcaatggta tctaaaatct tagaggcggt gggggggtgg atcgtggctt tgatttcctc
     1681 ctccggctac gcgggagtgg tcatcgccat gaccattgag tcggcctgca ttccgctccc
     1741 ttccgaaatc atcatgccct ttgcggggta tctggtcttc accggtcggt tcactatgct
     1801 cggggcgtcg gtggcagggg cgttcgggtg tctggtcggc tccattctgg cctacggcat
     1861 tggctactac ggcggcaggc ctttggccgt gaagtacggg cgctacgttt tgctctccca
     1921 acacgatctg gacaaagccg accggtggtt cgcccgctgg ggcaacagcg tgattttctt
     1981 ctcgcggctt ctgccgatcg tccgcacatt catttcgttc cccgccggca tcgcgcggat
     2041 gccgttctgg cgcttttgca tttactcttt cctcggttcc ctcccctggt gctgggcttt
     2101 ggcctacctg ggcaaaaaaa tgggcgagaa ctgggtggca atccggcagt ggttccacga
     2161 gatcgattac gtcatcggcg ggctgatcct tttgggcctc gtccttttca tctaccggca
     2221 cctgcgggga gcgcgggagg cggtccaagc accggaatga aaagacggat tggggatata
     2281 ggagttggga tttaggggaa aaccgaaata cgacagctgt tcttcaaacc gggttgattg
     2341 cttcgtcagc tgctggagca taaattaggc ggccaggata gttttggtag gcagaattgt
     2401 gaagaaagcg attcaaaaac ccctctggac ctgtcccaag tgccggaaaa aattcgtcac
     2461 ccgcaacctg tggcattcct gcggcaattt taccgttgac cagttttttc aagggaagag
     2521 cccgcacgcg aagaaacttt ttcaaaaatt tgtcgcgctg gctaaaaagt gcgggccgat
     2581 catcgtcaat ccgtcgaaga cgaggatcag tttccaggcc cggacgcgct ttgccggagt
     2641 gagcaaagcc agcgacgaag ggctcatctg cggttttctc ctgacccgcc gtttggagag
     2701 cccgcgcttt acctccattg aattctttcc gccgagatac tacggccacc ggtttcaaat
     2761 taaccggatg caagatttga atcaggaagt cctggggtgg ctgaaagagg cgtataaagt
     2821 gggccgacag gaacatttgc agaaaaagtc ccagtaatgt atatctctgg ccgggaagaa
     2881 tttcggggaa acaaaaaagg ctctcaaccg tacaaaacct taaaccaaga ggaggagctt
     2941 gatggacaaa cggtacaaag gttcagtcgg tttcgcagtt ctgttcgtcc tgctgagtgt
     3001 cgcggttatc tcagcacagc ccgcgcaacc cactaagaca cccagcttgc cctcgctgaa
     3061 gttcgaaaaa tacgttttgc ccaacggtct gcaggtgatc ctacaccaag accactcgat
     3121 cccggtggtg gcggtgaacg tctggtacca cgtgggctcc aagaacgaaa agctgggccg
     3181 caccgggttt gcccacctgt tcgagcacat gatgttccag ggctccgagc accagccggg
     3241 cgagtacttc gcgccgctgg aaaaagtggg cggctccgcc aacggctcca ccgccgaaga
     3301 ccgcaccgac tactgggaga acgtgcccgc caactacctt gaaatggcgc tctggctgga
     3361 agccgaccga atggggtacc tgttgccggc gatggatcaa aagaaattgg acaaccagcg
     3421 cgacgtggtg aaaaacgaga ggcgccaggg ggtcgataac gagccgtacg gcaaggccga
     3481 agagatgatc ccgtctctgc tctacccgat gggccacccc tattcctggt cggtgatcgg
     3541 cagccacgaa gacctaaccc gcgccagtct ggatgacgtg aaagatttct tccgaaactt
     3601 ctacactccc aacaacgcca gtctctgtat tgcgggcgat tttgatccga aaacggccaa
     3661 ggaattggtc aaaaaatatt tcggtgacat tccgccgggc ccttccgtgg aacggctgaa
     3721 aaactgggtt ccgacgctgg acggggagaa acggattgtg gtgcccgacc gggtctcgct
     3781 cccgcggctt tacctcttgt ggcagaccac accgttttac acccccggcg acgccgaatt
     3841 agatcttctg gggaacatcc tttcctccgg gaaaacttca cgattataca aatcgctggt
     3901 ctacgagaag cagattgccc aggatgtggc cgcttaccag gcctcgcgcg aaatgggcag
     3961 caattttgcc gtcgtcgtca ctgccaagca ggggcacacg ctggaggaaa ttcaaaaggc
     4021 agtcgatcag gaattggcgg ccatccgtac caccggcgtg actccggcag aactgatcca
     4081 ggcgcagaac aacttcgaag ccaatttcat gcggcaactg cagaacgtcg gcagtttcgg
     4141 cggacgggcc gaccttttga accagtacaa cacctactta ggcgatccgg ggaaactcca
     4201 gtgggacctc gaccggtacc tcaaggcgac gccggaaggg gtcaagcagg tggcggagaa
     4261 gtacctcgac ccgaaacgcc gcgtactcct gaccgtagtg ccgcaagaag actatcagcc
     4321 ggaagcagtc acggtcgatc gctcaactca gccgccggcg gggccggagc cgaccttcac
     4381 tccgcccaag gtgcagcgcg ccaagctctc caacgggatg gacgttcttt tagtcgaaga
     4441 gcacaaactg ccgctggtgc aggtggaatt ggtgctcaag tctggctggg cggccgaccc
     4501 aaaggaacgt ccgggcgcag ccagtctcac cgccgaactc ttagatgagg ggacgacgac
     4561 caagaatgcg ctccagattt ccggtgaggt gaaaagtttg ggcgcccagc tggggacagg
     4621 cagtttcttc gacgcttcca ccgccagcat caacctcctc aagaagcact tagacaaagg
     4681 gctttcgatt ctttccgacg tgatcatgca cccgaccttc ccggcggaag agttggaacg
     4741 ccagcggcag atttacctgg ggcgcattct gcaggaatcg aaggagccgt tcaccagcgg
     4801 ctttaaggtt ttcctgcgca cgctctacgg gccgggccac ccctacggcc agccctacac
     4861 gggcagcggt accgaaactt cgatcaaggc cattgggcgt tccgatctaa ccaaatacta
     4921 ccagacttac tacgcgccga acaacgcggc cttggtggtg gtgggcgaca tcacgctcgc
     4981 cgaagcgctg cccaagttag aggccgcttt caaagaatgg aaaccgaaag aggtgcccaa
     5041 ggtggagatc ccgccggtca agccgctcac cgcccccaag gtcttcatcg tcgataagcc
     5101 cggagcggcg cagtcggtga ttttgatggg caactttgcc ctggcgcgga aggcgcccga
     5161 ctacattccg gcgcaggtga tgaataacgt
//
LOCUS       DBNGZA010000030         5759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1702005, whole genome shotgun sequence.
ACCESSION   DBNGZA010000030 DBNGZA010000000
VERSION     DBNGZA010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1702005"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="rplQ"
                     /locus_tag="AC16FU_00560"
     CDS             complement(<1..474)
                     /gene="rplQ"
                     /locus_tag="AC16FU_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR2992905.1"
                     /translation="MRHRRYVFKLGRTKSHREAMLANLATALLEHRRIRTTAVRARAL
                     VPFVERLITLAKAGDLAARRQAAVVVRSPLVLQKLFSEIAPELASRNGGYTKVLKLGI
                     RQGDGASLALVELLVTRPKPKVAEKKKGAKKSEKETEAKAAPESKLEKGRKSKEKY"
     gene            complement(471..1457)
                     /locus_tag="AC16FU_00565"
     CDS             complement(471..1457)
                     /locus_tag="AC16FU_00565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR2992906.1"
                     /translation="MKWKNMQIPKEVTVDPETATAQYSRLVIEPLERGFGTTLGNALR
                     RVLLSSLPGAAVTALRIDGVVHEFTTVPGVYEDVPEIVLNVKRVRFRANADEPRTLLL
                     EANRKGKLTAAQIQSDGTYEVLNKDQFICQLTGDAKFRCEIDVGVGRSYVAAEQNKRP
                     SAPVGTIFLDSFFSPVTKVNFEIENTRVGQRTDYERLILDVWTDSSVTPDAAVNLAAK
                     LLREHLALFVKAEEELEIQPAEPEDEDVLRVRNLLKMRVDELELSVRSANCLRAEGIE
                     TLEQLVHRTEGQMLKCRNFGKKSLTELNQILGELGLKFGMDLSRYYQKELVA"
     gene            complement(1483..2112)
                     /gene="rpsD"
                     /locus_tag="AC16FU_00570"
     CDS             complement(1483..2112)
                     /gene="rpsD"
                     /locus_tag="AC16FU_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR2992907.1"
                     /translation="MARYTGPSCRLCRREGEKLFLKGQRCSSDKCAFDRRGYAPGQHG
                     ADSRRKRSDYAMRLREKQKVRRIYGLLEAQFRISFAKAERGPGITGENLLALLERRLD
                     NVVYRLGFAPSRKAARQLVRHRHFTVNDRTVDIPSYLLAPHDVVRVREESRALDVIHA
                     ALKDQGREQTVPYLRLDKARLEGELLEVPKRADIPTLVSEQLIVELYSK"
     gene            complement(2124..2516)
                     /gene="rpsK"
                     /locus_tag="AC16FU_00575"
     CDS             complement(2124..2516)
                     /gene="rpsK"
                     /locus_tag="AC16FU_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR2992908.1"
                     /translation="MQKPQAKKPKKRQRRVDTNGVVHIKATFNNTIVTITDTAGHVIC
                     WASAGKSGFQGSKKSTPFAAQVAAERCARDAMDQGLRRVEVWVKGPGSGREAAIRSLA
                     AAGLEVTAIRDVTPIPHNGCRPPKRRRV"
     gene            complement(2532..2951)
                     /gene="rpsM"
                     /locus_tag="AC16FU_00580"
     CDS             complement(2532..2951)
                     /gene="rpsM"
                     /locus_tag="AC16FU_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR2992909.1"
                     /translation="MARIAGVDLPKERRIDAALPYLYGIGWPSARRILARTGINPGTR
                     VHQLTEEEIAKLRAAIESDYKVEGALRSEVAMNVKRLIDIGSYRGLRHRRSLPVRGQR
                     TKTNARTRKGPKKTIGRLRKEAPEPAKVAPTGGEKKG"
     gene            complement(2967..3080)
                     /gene="rpmJ"
                     /locus_tag="AC16FU_00585"
     CDS             complement(2967..3080)
                     /gene="rpmJ"
                     /locus_tag="AC16FU_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR2992910.1"
                     /translation="MKVRSSIKKRCEHCKIVRRRGVLFVICKNPRHKQRQG"
     gene            complement(3109..3327)
                     /gene="infA"
                     /locus_tag="AC16FU_00590"
     CDS             complement(3109..3327)
                     /gene="infA"
                     /locus_tag="AC16FU_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR2992911.1"
                     /translation="MPKEETIQVEGKVLEPLPNAMFRVELDNGHKILAHVSGKMRMHF
                     IKILPGDKVTVELSPYDLSRGRIVYRYK"
     gene            complement(3329..4108)
                     /gene="map"
                     /locus_tag="AC16FU_00595"
     CDS             complement(3329..4108)
                     /gene="map"
                     /locus_tag="AC16FU_00595"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR2992912.1"
                     /translation="MELKSPTEIGLMRRAGASVARTLALVEQLVGPGITTGELNDRAE
                     TLIRQEGGVPAFLGYHGYPKAICVSIDDEVVHGIPGSRRLKSGQIVSVDVGVALDGYF
                     ADGAATFAVGRVSDEAARLLAVTQKALAAGIAQAKAGNRLSDISAAVQGTVEENGFSV
                     VRDLVGHGIGRQMHEEPQVPNFGRPGEGPTLAVGLVLAIEPMVNAGGYAVAVKPDRWT
                     VVTKDGSLSAHFEHTVAITDNGPEILTWFGELTTLSKVAGR"
     gene            complement(4141..4815)
                     /locus_tag="AC16FU_00600"
     CDS             complement(4141..4815)
                     /locus_tag="AC16FU_00600"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR2992913.1"
                     /translation="MWLVLLGPPGSGKGTQAKELASRFGYQSIATGDLLRSEVEKETE
                     LGQAAKKYMDAGALVPDEVILAMLALQIPKALGGVIFDGFPRTVAQAEGLDQVGERLG
                     ARVHLAIKLDVPDDEIIRRLAARRLCPACGASYNTETQPPRTPGCCDHCGAALVSRND
                     DLPEVVANRLAVYRRETTPVEAYYQKRGLLRTVNGNRPRAEVERALEALVAECKKQAA
                     SPTSRP"
     gene            complement(4822..>5759)
                     /gene="secY"
                     /locus_tag="AC16FU_00605"
     CDS             complement(4822..>5759)
                     /gene="secY"
                     /locus_tag="AC16FU_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR2992914.1"
                     /translation="LGTAVYLESLWVPGTNALIVPHPGMLFKFLCMITFSSGTIFIMW
                     LGEQITEKGIGNGISLIIFIGIVARLPQSIVDEVVELSAGRRSVVVELLLIAIIVGVI
                     AAIVLVTRAQRRIPVQYAKRVVGRRVYGGQSTHIPLSVNTAGIIPIIFAQSILFAPGT
                     LSSFFPNVQFMQDVAAAFRPGAVLYSLVYALLILFFAYFYTAIVFNPIDLADNMRKYG
                     GFIPGIRPGKHTSDYIDRVLSRITLPGALFFAAIAILPYIFIKQLNVSYYFGGTGLLI
                     VVGVALDTLQQVESHLLMRHYEGFMKKGRIKGRGF"
BASE COUNT         1016 a         1855 c         1707 g         1181 t
ORIGIN      
        1 gtatttttcc tttgacttcc tgcctttttc caacttactc tctggcgccg ctttggcttc
       61 ggtttctttt tcgctcttct tggcgccttt ctttttctct gcgactttcg gcttggggcg
      121 ggtgaccaaa agctccacca aagccaggga cgccccgtcg ccctgccgga ttcccagctt
      181 caacactttg gtgtacccgc cgtttcggct ggccagttcg ggggcgattt cgctgaagag
      241 tttctggagc accaggggac tgcgcacgac caccgccgcc tgacgccgcg ccgccagatc
      301 tccggctttg gccagagtga tcagccgctc cacgaaaggc accagggctc gcgctcgcac
      361 ggcggtggtc cgaatcctcc ggtgttccag aagtgccgta gccagattgg cgagcatggc
      421 ctcgcggtgg cttttggtcc ggcccagctt gaaaacgtag cgccggtgcc tcacgcgact
      481 aactctttct ggtagtaccg ggagagatcc atcccgaact tgaggccgag ttcacccaag
      541 atctgattca gttcagtgag cgatttcttc ccgaaattac ggcacttgag catctgtcct
      601 tcggtgcggt gcaccagctg ctccaaggtc tcaatgcctt cggcccgcag gcagttggcg
      661 gagcggacgg agagctccag ttcgtccacc cgcattttca ggaggttccg gacgcggagc
      721 acgtcctcgt cctccggttc cgccggttga atttctaact cctcctccgc tttgacgaag
      781 agtgccaggt gctcccgcag gagtttggcg gctaagttca ccgccgcgtc cggggtgacc
      841 gaactgtcgg tccagacatc caggatgagc cgttcgtagt cggtgcgctg acccacccgt
      901 gtattctcga tttcgaaatt aactttggtt accggcgaga agaacgaatc cagaaagatc
      961 gtccccaccg gcgccgaggg cctcttgttc tgctcggcag ccacgtagct gcggccaacg
     1021 cccacgtcga tctcgcagcg aaacttggcg tcgccggtca gctggcaaat gaactggtcc
     1081 ttgttgagaa cctcgtaggt gccgtcgctc tgaatctgcg cggcggtgag ttttcccttg
     1141 cggttcgctt ccaaaaggag cgttctcggc tcgtcggcgt tggcccggaa gcgcacccgc
     1201 ttgacgttga gcacgatttc cggcacgtct tcgtagacgc cgggcaccgt ggtgaactca
     1261 tgcactaccc cgtcaatccg cagtgcggtg acggccgcac cgggcaaaga agacagaagc
     1321 acccgccgga gcgcattccc cagtgtggtc ccgaaccccc gctccagggg ctcgatcacc
     1381 agacggctgt actgggcggt tgcggtttcg ggatcgaccg tcacctcttt gggtatttgc
     1441 atgttcttcc acttcatggg cgcgactcct ctgccgaaag catcatttcg aatacaattc
     1501 gacgatcaac tgctcggaga ctaacgtggg aatatcggcc cgcttgggga cctccaggag
     1561 ctctccctcc aggcgggctt tgtccagacg caggtagggc acggtctgct cccggccctg
     1621 gtctttgagg gccgcgtgga tgacgtccag ggcacggctt tcttcccgga cccgcacgac
     1681 gtcgtgcggc gcaagcaggt acgacgggat gtcgaccgta cggtcgttca cggtgaaatg
     1741 ccggtggcgc accagttgcc gggccgcctt gcgcgagggc gcgaacccca gccggtagac
     1801 cacgttgtcc agccgccgct ccaaaagcgc caggagattt tcacccgtga tgcccggacc
     1861 ccgctccgct ttggcgaaag agatgcggaa ctgggcctcc aggagcccgt agatgcggcg
     1921 caccttctgt ttttccctga ggcgcatggc atagtcggac ctcttccggc gcgaatcggc
     1981 accgtgctgg cccggagcgt acccccggcg gtcgaacgca cacttatcgg aagagcagcg
     2041 ctggcctttc agaaagagtt tttcaccttc ccggcggcag aggcggcatg agggtccggt
     2101 gtatctggcc atcggctcct ccgctacacc cggcgccgct tgggcgggcg gcagccgttg
     2161 tggggaatcg gcgtcacgtc gcggatggcg gtgacttcca gccccgccgc cgcgagcgac
     2221 cggatggccg cttcccgtcc cgagccgggg cctttgaccc agacttcgac ccggcgcaaa
     2281 ccctggtcca tggcatcccg cgcgcagcgc tccgccgcca cctgagcggc aaagggcgtg
     2341 ctcttcttgg aaccctggaa gcccgacttc ccggccgagg cccagcaaat gacgtggccg
     2401 gcagtgtcgg tgatcgtgac aatggtattg ttaaacgtcg ccttgatgtg caccacgccg
     2461 ttggtatcga cccggcgctg gcgttttttg ggctttttcg cttgcggttt ttgaatctct
     2521 tcagccacgc actatccttt cttttctccg ccggtcgggg ctaccttcgc gggttccggt
     2581 gcttctttcc tgagccggcc gatggttttc ttgggtcctt tgcgggtccg cgcgttggtc
     2641 ttggtcctct gcccgcgcac cggcaagctg cggcggtgcc gcaggccgcg gtacgagccg
     2701 atgtcgatca gccgcttgac gttcatcgcc acttccgagc gcagggcgcc ttcgactttg
     2761 tagtccgact caatcgccgc gcgcagtttc gcgatttctt cttcggtcaa ctggtgcacc
     2821 cgcgtgccgg gattaatccc cgtacgggcc aaaatccgac gggccgacgg ccagccgatg
     2881 ccgtagaggt acggcagggc cgcgtcaatc cgcctctcct tcggtaagtc cactccagcg
     2941 atgcgcgcca tttgtttctt tcgctcctac ccttgccgct gcttgtgccg ggggtttttg
     3001 cagatgacga aaagcacccc gcggcggcgg acaattttac agtgctcaca gcgtttctta
     3061 atcgacgagc gtactttcat gaacgacctt cgctcccgtg tcccagatct acttgtaacg
     3121 gtagacaatc cgcccgcgcg acaggtcgta gggcgagagt tccaccgtca ccttatcgcc
     3181 ggggagaatc ttaatgaagt gcattcgcat tttccctgac acgtgcgcca gtattttgtg
     3241 cccgttatcc agctctacgc ggaacatcgc attcggtaac ggttcgagta ctttcccttc
     3301 gacctggatc gtttcttctt tcggcatctt accgcccggc gactttgctc agggtggtga
     3361 gctcgccgaa ccacgtcagg atctccggac cgttgtcggt aatggccacc gtgtgttcga
     3421 agtgggccga gagcgatccg tctttggtca ccacggtcca gcgatccggt ttcaccgcca
     3481 cggcgtagcc acccgcgttc accatcggct cgatggccag aaccaggccg acggccagcg
     3541 tcgggccctc gccgggccgc ccgaaattcg gcacctgcgg ttcctcatgc atctggcggc
     3601 cgatcccgtg acccaccaga tcgcgcacga cggaaaatcc gttttcttcg acggtgccct
     3661 gaaccgccgc cgaaatgtcg gagaggcggt tgccggcttt ggcctgggca atgccggctg
     3721 ccagtgcctt ctgggtgacg gcgagcaggc gcgcggcttc gtcggaaact cggcccaccg
     3781 caaaagttgc cgcaccgtcg gcgaagtagc cgtccagggc cacgccgacg tcgacggaaa
     3841 cgatttgccc ggacttgagg cgccggctcc ccggaatgcc gtgcaccact tcatcgtcaa
     3901 tggagacgca gatcgctttc gggtacccgt ggtagcccaa aaacgccggc acgccgcctt
     3961 cctggcggat caacgtctcg gcgcgatcgt tcagttcgcc ggtggtgatc ccgggaccga
     4021 ccagttgttc gaccaatgcc agcgtgcgcg ccacgctggc gccggcccgg cgcatgagcc
     4081 cgatttcggt gggcgatttc agctccattg ctgatccgat caaaaccgct tgcgcccgcg
     4141 tcacgggcgc gacgtcggcg acgccgcctg ttttttgcat tccgctacca atgcctccag
     4201 agcccgctcg acctccgccc gcggccggtt tccgttcacc gtacgcagga ggccgcgttt
     4261 ttgataatac gcctccaccg gggttgtctc ccgtcgatag actgccagcc ggttggcgac
     4321 tacttcgggc aggtcgtcat tgcggctgac caaggccgcc ccgcagtggt cgcagcaacc
     4381 cggcgtgcgc ggcggctgcg tctccgtatt ataactcgca ccacaggccg gacacaagcg
     4441 ccgggcggct aaacgccgga taatttcatc atcaggcacg tccagtttga tggccaaatg
     4501 cacccgtgcg ccgaggcgct ctcccacctg atccagaccc tcggcctgcg ccacggtgcg
     4561 cgggaagccg tcgaagatga ccccgcccaa agctttggga atctgcaagg ccagcatcgc
     4621 taaaatcact tcgtccggaa ccagggcgcc cgcatccatg tactttttgg ccgcttgacc
     4681 caactcggtt tccttttcga cttcgctccg cagcaaatca cccgtcgcaa tactctggta
     4741 cccgaaacgg gacgccaatt ccttcgcctg tgtgcctttg cccgatccgg gcggccccaa
     4801 gagcacgagc cacatccggc gtcagaaccc ccgtcctttg atccgtccct tcttcatgaa
     4861 tccttcgtag tgacgcatca aaaggtgcga ctccacctgc tgcagcgtat ccagcgccac
     4921 ccccaccaca atcaaaagcc cggtgccgcc gaagtagtag ctgacattga gctgtttgat
     4981 gaaaatgtag gggaggatgg cgatggcggc gaagaagagt gcccccggca gagtgatgcg
     5041 cgaaagcacc cggtcgatgt aatcggaggt gtgcttcccc ggccggatgc ccgggatgaa
     5101 gccgccgtac ttgcgcatgt tgtccgccag gtcgatcggg ttgaagacaa tggcggtgta
     5161 gaagtaggca aagaataaga tcaagagcgc gtaaactaac gaatagagaa ccgctcccgg
     5221 gcggaaggcc gctgccacat cctgcatgaa ctggacattg gggaaaaagg aggagagcgt
     5281 cccgggagcg aagaggatcg actgggcaaa gataatcggg atgatgccgg cggtgttgac
     5341 cgaaagcgga atgtgggtgc tctgcccgcc gtaaacccgc cgcccgacca cccgttttgc
     5401 gtactgcacc ggaatccggc gctgggcccg ggtcaccaga acgatcgccg caatgacgcc
     5461 gacaataata gcgatcaaga gaagttcgac cacgacgctc cgccgcccgg cggagagctc
     5521 caccacctcg tccacgatcg actggggcaa acgggcgacg atgccaatga agatgatgag
     5581 ggaaatcccg ttgccgattc ccttctcggt gatctgttct cccagccaca tgatgaagat
     5641 ggttccggag ctgaaggtga tcatgcagag aaatttaaac aacatacccg ggtggggtac
     5701 gataagggca ttggtgccgg ggacccacag gctctcgagg tagaccgccg tccctaagg
//
LOCUS       DBNGZA010000031         6073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_429880, whole genome shotgun sequence.
ACCESSION   DBNGZA010000031 DBNGZA010000000
VERSION     DBNGZA010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_429880"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="AC16FU_00610"
     CDS             complement(<1..369)
                     /locus_tag="AC16FU_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR2992915.1"
                     /translation="MSPVMTAERPEKIVPKEINPFANAQMQLDSAAKILALEPTLAEA
                     LRWPERELWVRIPVRMDNGKIKSFTGFRVQYNDARGPCKGGIRFHPDETIDTVRALAA
                     WMTWKTALLDLPLGGAKGGVI"
     gene            complement(761..1912)
                     /gene="ftsZ"
                     /locus_tag="AC16FU_00615"
     CDS             complement(761..1912)
                     /gene="ftsZ"
                     /locus_tag="AC16FU_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR2992916.1"
                     /translation="MFDFDFEHDGFAKIKVVGVGGAGGNAVNRMIDAKLSGVDFITIN
                     TDAQVLAASKAPHRVQIGTDLTKGLGAGANPEIGRKAIEEDRDTVREMLAGADMVFVT
                     AGMGGGTGTGAAPIVAEIGRQAGALTVGIVTKPFEFEGRKRMQRAFDGLEELKCRVDT
                     LLVIPNQKLLSIVAKDVSLNDAFRIADDVLMQATRGISELITVPGLINCDFADVRTVM
                     AEMGDAIMGTGVGQGENRAHLAAQGAISSPLLENVCIAGAKGVLVNVTGGPEMTLHEV
                     NEATSIVYKAAGEEANIIFGAVVDQNLADEMRVTVIATGFGSIRSDYAHPAMLREVDL
                     FRPVRRAAAAKQETANGYEKAEPVPVEVCPKIDVAELEVPTFLRRKLDR"
     gene            complement(1931..3166)
                     /gene="ftsA"
                     /locus_tag="AC16FU_00620"
     CDS             complement(1931..3166)
                     /gene="ftsA"
                     /locus_tag="AC16FU_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR2992917.1"
                     /translation="MVGAETVAALDLGTTKVSVLVAEVERDLWPKLLGIGVVSAAGIR
                     QGHVVDLPGATHSIRQAVQDAELMAGIKITRVVVGISGEQIQNFHSRSVVSVGTPRLR
                     QEVSPADVGRVLAAARLLEFPSDRQVLHVLPTRFAIDDQDGIRQPAGMVGVRLSADVQ
                     VVTCGRTATENLLRAVGKTELEVSELVAEPLAASRAVLTPEEKETGVALIDLGGSTTQ
                     LAVFLEDALALTACIPLGGRNATNDLAVGLRLPGDKAEELKLIHGAAYAPAVDPDEVV
                     LIPATGSRPPKQISRTVLASIIQPRLEEIFGLLARQIRRKSYAEPVPASVVLTGGGAL
                     LAGVCHLAEHCFDLPVRIGIPQGFTGLVERAAHPRHATGVGLVLYGAASPRQNGHRPP
                     HWERTVRKLTRVWREYFQP"
     gene            complement(3153..3914)
                     /locus_tag="AC16FU_00625"
     CDS             complement(3153..3914)
                     /locus_tag="AC16FU_00625"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR2992918.1"
                     /translation="MTPKREIFFRVLIVFGLLLAVGTATAERAGLFRLRRVMVSGNTV
                     VSREEILQRAKLPFGANLFRVDIEKIARRVAADQRFWGVTVSRQLPDAVEVSVTEKKP
                     ALTLNTGQLWGLTANLEVLPEEEASLSLPVVTAGYWQPDRFYQSNRQNGLGLAAEAYR
                     ALVACDSRLLEAISEISVAPSGDVVLYLVPKGAAVNFGTGDFSIKVKRLAALLAEFGG
                     ILPDLAYDLRFAGNLFVTPKDSLSVAKARSRRGRG"
     gene            complement(3911..5293)
                     /gene="murC"
                     /locus_tag="AC16FU_00630"
     CDS             complement(3911..5293)
                     /gene="murC"
                     /locus_tag="AC16FU_00630"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR2992919.1"
                     /translation="MSLRKIKRLHFVGIGGTGMCGIAEVLLSSGYQITGSDLKFSEAT
                     DRLVKLGAVVYAGHRAEQVRDAQVVVISSAVQPDNPEVQYALSHGIPVIRRAEMLGEL
                     MRMKFGIAVAGTHGKTTTTSMIGEILTTAGLDPTVVVGGRVVNLQTHAQIGGSEYLVA
                     EADEFDRSFLRLTPQVAVLTNIEADHLDCYKDIEDIKATFIQFANQVPFYGTVVLCAD
                     DANVAIIRPQISRPVVTYGLSGAVDFSAVDLAFSERRSAFNVFRGVEHLGQVALAIPG
                     VHNVKNALAAIAVGFEVDIPWEKIKTGLEAFRGVHRRFEVVGDVNGILVVDDYAHHPT
                     EIAATLAAARQGYPQRRRVAVFQPHLFTRTRDFFREFGEALRGADVAIVTDIYPSREK
                     PLPGITGQMVADAAVKAGHKQIHYVPDKNRVTGFLGSRLLPGDLVITLGAGDIYRVGL
                     ELVELLKTTV"
     gene            complement(5361..>6073)
                     /locus_tag="AC16FU_00635"
     CDS             complement(5361..>6073)
                     /locus_tag="AC16FU_00635"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR2992920.1"
                     /translation="VRPDIASGKREEAVKVFGLDPAKKTVLVIGGSQGAASINRALLR
                     SLDQFETESHVQFVWQTGKSLFAEVSRSVAGRKFTAKIFAFIDHMLLAYAAADLVVCR
                     SGALTLAELAVCGKPAILIPYPHAAADHQRHNAQVVQKAGAAEMILDFELEKISLGER
                     IMSLLHDDGRLKRMAEKSRALARPDAATTIVGLMDRLLAKAPQANPVARTRTEMQNLP
                     SIALESPALKLGITLFRR"
BASE COUNT         1180 a         1929 c         1762 g         1202 t
ORIGIN      
        1 aatcaccccg cctttcgccc cgcctaatgg caaatcaagt aaagcggttt tccaggtcat
       61 ccaggcggcc agggcccgca ccgtgtcaat ggtttcgtcc ggatggaagc ggataccccc
      121 cttgcacggc ccgcgggcgt cgttgtactg cacccggaag ccggtgaagg acttgatctt
      181 gccgttgtcc attcgcactg gaatacggac ccagagctct cgctccggcc agcggagtgc
      241 ctcagccagc gtcggttcta acgctaaaat ctttgcggct gaatcgagtt gcatttgggc
      301 attggcaaaa ggattgatct cttttgggac aattttctct ggacgttcgg cggtcattac
      361 tggtgacatt attcctcccc gggttacatg tctcatgcgt agctttgaag agtcaaagac
      421 ctggtcgatt ttcagaccca cttctatccg tacggaccga actttttatg gacgttggcc
      481 gcttctgact tgggacgaat accgccacca gcgcgatttt caggcggtct gcagagaaag
      541 gtttcattcg aactccgata attacgtctt aaaataggcg gcgagagaca ggatgtcaac
      601 cgtttttttg atggaaaagg attcaagcca aactctgtcg ggtatggggg tgggggctca
      661 ggagggccga atccactccc gccacgcaaa aacccccggg aaaaggctca tcgggggctc
      721 ttgttttaag cggtacgatc cgctgtctgt ttgctgtacg ttagcggtcc agcttccgcc
      781 gcaggaaggt cggcacttcc aactcggcca cgtcgatttt cgggcacact tccacgggaa
      841 ccggctcggc cttctcgtag ccgttggccg tctcctgttt ggcggccgcc gcgcgccgca
      901 ccggacggaa gaggtcaact tcccgcagca tcgccgggtg ggcatagtcc gagcgaatgc
      961 ttccgaagcc ggtggcgatc accgtcaccc gcatttcatc cgcgagattc tgatccacca
     1021 ccgcgccgaa gatgatgttc gcctcttccc ccgccgcctt gtagacgatg gaagtggcct
     1081 cgttcacctc gtgcagggtc atttccggcc cgccggtgac gtttaccagc acacctttgg
     1141 ccccggcgat gcagacgttt tccaaaagcg gactggagat cgccccttgc gccgccagat
     1201 gggctctgtt ctctccctgg ccgacgcccg tgcccatgat ggcgtcgccc atttcggcca
     1261 tcaccgtgcg cacgtcggcg aaatcgcagt tgatcagccc cggcacggta atgagttcgg
     1321 aaatcccccg cgtcgcttgc atcaaaacat cgtcggcgat ccggaaggcg tcgttgagcg
     1381 aaacgtcttt ggccacgatg gagaggagtt tctgattggg gatgaccagg agcgtatcga
     1441 cccggcactt cagttcctcc aacccgtcaa aggcgcgctg catccgtttg cgcccttcga
     1501 attcgaacgg tttggtgacg atgccgaccg tgagggctcc ggcctggcgg ccaatttcgg
     1561 ccacgatcgg cgccgcgccg gtgccggtgc cgccgcccat cccggcggtg acgaagacca
     1621 tgtcggcgcc ggccaacatt tcgcgcaccg tgtcgcggtc ttcttcaatc gccttgcgcc
     1681 cgatctccgg attggccccg gcgcccagcc ccttggtcag gtcggtgccg atctgtaccc
     1741 ggtggggtgc cttggaggct gccaaaacct gcgcgtcggt gttgatggtg atgaaatcga
     1801 cgccggagag tttggcatct atcatccggt tcaccgcatt cccgccggcg ccgcccaccc
     1861 ccaccacttt gattttggcg aaaccgtcgt gctcgaagtc gaagtcaaac agcatcgaac
     1921 ccctcccggg ttaaggttga aaatactcgc gccacactct cgtcaatttc cttacggtgc
     1981 gctcccagtg cggcggccgg tgcccgttct gccgcggcga ggcggccccg tacaaaacca
     2041 ggcccacgcc ggtggcgtgc ctcggatgcg ccgcgcgctc caccaggccg gtgaagccct
     2101 gcggaatgcc gatccgcacc ggtaaatcga aacagtgttc ggccaaatga cagaccccgg
     2161 ccagaagcgc accgccgccg gtgaggacga ccgaggccgg caccggctcg gcgtacgatt
     2221 tgcgccggat ctgccgggct aaaagcccga aaatctcttc taagcgcggc tgaatgatac
     2281 tggccagaac cgtccgcgaa atttgcttgg gcggacggct gcccgtggcc gggatcaaaa
     2341 ccacttcatc aggatcaacg gcgggggcat acgccgcacc gtgaatcagc ttcaactctt
     2401 cggctttgtc gccgggcaag cgcaagccca ccgccaggtc gttggtggca ttccgcccgc
     2461 ccaggggaat acaggcggtc aaggccaggg catcctccaa gaaaacggct aactgcgtgg
     2521 tgctgccgcc caggtcgatc aaagcgaccc ccgtttcctt ttcctccggc gtcaaaacgg
     2581 cccggctggc agccaaaggt tcagccacca gttcgctcac ctcgagttcg gttttcccta
     2641 cggcccgcag caaattttcg gttgccgtcc ggccgcaggt gacgacctgc acgtcggcgg
     2701 aaaggcgcac gccgaccatg ccggccggct ggcggatccc gtcctggtcg tcgatggcaa
     2761 agcgggtcgg caagacgtgc agcacctgac ggtcggaggg aaattccaaa agccgggccg
     2821 ccgccaggac ccgcccgaca tctgcggggg aaacctcctg gcgtaaacgc ggggttccca
     2881 ccgacaccac gctccggctg tgaaagtttt gtatctgctc gccggaaatt ccgactacca
     2941 cacgggtgat tttgattccc gccatgagtt cggcgtcctg gaccgcctgg cgaatcgaat
     3001 gggttgcccc gggaaggtct accacatgtc cttgccggat tcccgcggcg gaaaccacgc
     3061 cgatacccag gagtttcggc cagagatcgc gttccacttc ggccacgagc acgctcacct
     3121 tggtggtgcc caaatctaag gcggcgaccg tttcagcccc gaccacggcg gctcctcgct
     3181 ttcgcaacgg aaagtgaatc tttcggcgtg acgaaaagat tgccggcaaa cctgaggtcg
     3241 taggccagat cgggcagaat gccgccgaac tcagccaaaa gcgccgccaa ccgcttgact
     3301 tttatcgaaa agtcgccggt gccgaagttg accgccgcac ccttcgggac gaggtagagc
     3361 accacatctc ccgacggcgc tacgctaatt tccgagattg cttccaagag acgggaatcg
     3421 caggcgacca gggcccggta cgcctctgcc gccagaccca acccgttctg ccggttcgat
     3481 tggtagaaac ggtcgggctg ccagtatccg gcggtcacga ccggcaggga aagggaggct
     3541 tcctcttccg gaagcacttc taagttggcc gtgagacccc agagttgccc ggtgttcagg
     3601 gtcagagccg gttttttttc ggtcacggaa acctcgaccg cgtcgggcaa ttgtcgcgaa
     3661 accgttacgc cccaaaacct ctgatctgcc gccacccggc gcgcgatttt ttcgatgtcg
     3721 acgcgaaaca gattcgcccc gaacggcagt ttcgctctct ggagaatttc ttcccggctg
     3781 accaccgtat taccgctgac catgactctg cggagccgaa acaacccggc ccgctcggcg
     3841 gtggcggtcc ccacagccag gagaagccca aacacgatga gaacgcgaaa gaatatttct
     3901 cgtttcggcg tcatacggtc gtcttaagca gctccactaa ttccagtcct acccggtaaa
     3961 tgtccccggc acccagggtt atgacgaggt cgccgggcaa aagccgcgat cccagaaagc
     4021 cggtgacgcg atttttgtcc ggaacgtagt gaatctgttt gtgcccggct ttgaccgccg
     4081 catctgccac catttggccc gtaatgcccg gcaaaggctt ctcgcgggag gggtaaatgt
     4141 ctgtgacaat agcgacgtca gcaccgcgga gtgcctcacc gaattcccgg aaaaagtccc
     4201 gcgtgcgggt gaaaaggtgc ggctggaaga ccgccacccg gcgcctttgg ggatacccct
     4261 gacgtgcggc tgcgagcgtg gcggcaatct cggtcgggtg gtgggcgtaa tcgtcaacga
     4321 ccagaatgcc gttaacatca cccacgacct caaaccgccg gtgcaccccg cggaacgctt
     4381 ccaggccggt cttgatcttt tcccacggga tgtcaacttc aaagcccacc gcaatcgccg
     4441 ccagtgcatt tttgacgtta tgtactcccg gaatggccag cgcaacctgg cccagatgct
     4501 cgacgccccg gaaaacattg aacgcagaac gccgctcact aaaagcgaga tcgacagccg
     4561 aaaaatcaac cgccccggaa agtccgtagg tcaccaccgg ccgggaaatc tgcgggcgaa
     4621 taatagcgac gttcgcatcg tcggcacaga ggactacggt gccgtaaaag ggaacctgat
     4681 tggcgaactg gatgaaagtc gccttgatgt cttcgatgtc cttgtagcaa tcgaggtgat
     4741 cggcttcgat attggtcagg accgccacct gcggcgtgag ccgcaaaaac gaacggtcaa
     4801 actcgtcggc ttcggctacc aaatactcgc tcccgccgat ctgcgcatgg gtctggagat
     4861 tcacgacccg gccgcccacc accacggtcg gatccagccc cgcggtggtc aggatttcgc
     4921 cgatcatcga tgtcgtggtg gtcttcccgt gtgtcccggc cacggcaatg ccgaatttca
     4981 tccgcatcag ctcgcccagc atctccgccc gccggattac cggaatgccg tgggaaaggg
     5041 cgtactggac ttcgggattg tccggttgca ccgccgagga aatgaccacc acctgcgcat
     5101 cccgcacctg ctccgcccgg tggcctgcgt agaccaccgc ccccagtttg accagccggt
     5161 cggtcgcctc cgaaaacttg aggtcggagc cggtgatctg gtagccggag gagaggagca
     5221 cctcggcgat gccgcacatc ccggtgccgc cgatgccgac gaagtgcagg cgcttgatct
     5281 tccgcaggct cataccgatt cgtttgggtg tgacactctt tttcctgacg accgtctcga
     5341 tagtatccat aggatggggt tcatctccgg aagagggtga taccgagttt caatgcgggc
     5401 gactcgagcg cgatagatgg gagattctgc atttcggtac gggtgcgggc caccgggttc
     5461 gcctgcggtg ccttggccag caaccggtcc atcaaaccga caatggttgt cgccgcatcg
     5521 gggcgggcca gcgcccggct tttctcggcc atgcgcttca gtcggccgtc gtcatgcagg
     5581 aggctcatga ttcgctcgcc cagactgatc ttctctaatt caaaatccaa aatcatttcc
     5641 gccgcacccg ctttttgcac tacttgcgcg ttgtggcgct ggtggtcggc ggcggcgtgg
     5701 gggtagggaa tgagaatggc gggcttgcca cagaccgcca gttccgccag agttagcgct
     5761 cccgaccggc agacgaccag gtcggctgcg gcgtaggcta aaagcatatg gtcgatgaaa
     5821 gcaaaaatct tggccgtaaa ttttcgacct gccacgctac ggctcacttc agcgaagagg
     5881 ctctttcccg tctgccagac gaactgaacg tgactttcgg tttcgaactg gtctagtgac
     5941 cgcaaaagcg cccgattgat ggaggccgct ccctggctgc cgccgatcac cagcaccgtt
     6001 ttcttcgccg gatccagtcc aaaaactttg accgcttctt cccttttacc cgacgcaata
     6061 tccggccgca ccg
//
LOCUS       DBNGZA010000032         8317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2762903, whole genome shotgun sequence.
ACCESSION   DBNGZA010000032 DBNGZA010000000
VERSION     DBNGZA010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2762903"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="cobO"
                     /locus_tag="AC16FU_00640"
     CDS             <1..530
                     /gene="cobO"
                     /locus_tag="AC16FU_00640"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR2992921.1"
                     /translation="RRGLVVIHTGDGKGKTTAALGMAVRAAGYKMKVFILQFVKGTWK
                     YGELEGVKLLAPYVEMEQKGKGFVGIIDDKYDISEHQKAGREALVYARKIVSEGEYDL
                     VILDEINVAIGLSLIPVADVVELVKSKPARLHLVLTGRGAPQELIELADTVTEMREIK
                     HAFRRGILAQKGIDF"
     gene            829..1542
                     /locus_tag="AC16FU_00645"
     CDS             829..1542
                     /locus_tag="AC16FU_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2992922.1"
                     /translation="MISEQFDSPHHLWEYSHFEDAANKAQEAQVPTGRYSREEAFWEV
                     LKTNPEPLPTVVRKTSFNTRMYHLGQSRFFSYLLSVCLLILALPLFLLIGFLLFVESP
                     GPVIVRVPRLGEDRRRLNRRGAEGIPRWDKRFRDRRRENLLGRPFTAFRFRTVSRAPA
                     EYVQALHRHPDTTWVGWVLEGTGLDHLPQLLNVLKGDLVLIGPRPEPLEIIHDLPEHA
                     GRYPTLLRVKPGFFVAGPI"
     gene            2126..3682
                     /locus_tag="AC16FU_00650"
     rRNA            2126..3682
                     /locus_tag="AC16FU_00650"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            3955..4031
                     /locus_tag="AC16FU_00655"
     tRNA            3955..4031
                     /locus_tag="AC16FU_00655"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3989..3991,aa:Ile,seq:gat)
     gene            4246..5460
                     /locus_tag="AC16FU_00660"
     CDS             4246..5460
                     /locus_tag="AC16FU_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992923.1"
                     /translation="MRTAKSFLWLVALGVVLSCAAVVWGTESRIATVGNNPLFLRDDI
                     GMFTFPSTAVDYRKHIFAEHLTNGGSGEIGVDQRDFFGGPISGRVGIVTNFVGQSALG
                     VFIGQQEEHFRGPLFPVDQPQTRFNLIYGKEMSKVTLGLNFNFSGVKTESRVGNVPTG
                     TLLQDKAGTFGFGAGLGVPLTETQDLQVAGHIELRNFSSDTGNVTVRQDDGNWSLLFL
                     GRDFIEYNDMVTLVPAGQVEIFNEKSRRVPGPQPDTAHEIKGTNWQFGLGCNVQPNEK
                     VLINSAVGVRGGSSQHKIDQNLTEAGTSKTSFFTIPFALLGMDVEVKKGLDFRAGATK
                     EIRSDKNNILNTKNTDTDFTYAIGAAVRLGDVTFDTRVANTFLNNGPYLLSGQTTFGG
                     MFPQVSFTYAWK"
     gene            5603..5675
                     /locus_tag="AC16FU_00665"
     tRNA            5603..5675
                     /locus_tag="AC16FU_00665"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5636..5638,aa:Ala,seq:tgc)
     gene            5926..>8317
                     /locus_tag="AC16FU_00670"
     rRNA            5926..>8317
                     /locus_tag="AC16FU_00670"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1969 a         2125 c         2630 g         1593 t
ORIGIN      
        1 agcggcgcgg tttagtcgtc atccacaccg gcgacgggaa ggggaaaact accgcggcct
       61 tggggatggc ggtgcgcgcg gcagggtata aaatgaaagt cttcatcctg cagttcgtca
      121 aaggcacctg gaaatacggc gaactggaag gcgtcaaact gctcgcaccc tacgtagaaa
      181 tggagcagaa ggggaaaggg ttcgtcggga tcatcgacga caaatacgac atttccgagc
      241 accaaaaagc aggccgggaa gcgctggtgt atgcgcggaa aatcgtttcc gagggggaat
      301 acgatctggt gatattggac gaaatcaacg tggcgatcgg gctcagtttg attccggtgg
      361 ccgacgtggt tgaactggtg aaatccaagc cggctcggct gcacctcgtg ctcacaggcc
      421 ggggtgcgcc ccaggagtta attgaactgg ctgacacggt gaccgaaatg cgcgaaatca
      481 agcacgcctt ccgccgggga atcctggcgc aaaaggggat cgatttctaa gcgtccgggg
      541 gtcggcaggt gctgaaattt cctgcgcagc aggctcgtga tttgctggcc ggtttctggg
      601 tgcaatagcc ggccaacctt tttcgttgat tgcttccgat tctgaagttt cttgcatcgg
      661 gaatcaaatc tccccggaac tgagaagcag tggtcaaatt ttgaccaccg cttactaatt
      721 atcctttaat aagttaccct gattaataat agagttagat ttgagtcctg atgggcaaag
      781 gcgttgcgga tatatgatag tgccaaaagt atgagaggac gtgagtagat gatatccgag
      841 cagtttgata gtccgcacca cctgtgggaa tactcccact ttgaggatgc cgccaacaag
      901 gctcaggaag ctcaagttcc caccgggaga tattcacgag aggaagcttt ctgggaagtg
      961 ctgaagacca accccgaacc gctgcccacc gttgtcagga agacttcatt caacacccgc
     1021 atgtaccacc tggggcagag ccgattcttc tcgtaccttc tttcggtttg ccttttgatt
     1081 ctggccttgc cgctttttct tttgatcggc tttttgctct tcgtcgagtc gccggggccg
     1141 gttatcgtcc gcgtgccgcg tttgggagaa gaccgcaggc gcttgaaccg ccgcggggcg
     1201 gagggaattc cccgctggga taagcgcttc cgggatcggc ggcgggaaaa tcttctggga
     1261 cggccgttca ccgccttccg cttccgcacg gtcagccggg ctccggccga gtacgtccaa
     1321 gcgctccacc ggcatccgga cacgacctgg gtcggctggg ttctggaagg aacgggtctg
     1381 gatcatctgc cgcagttgtt gaatgtcctc aaaggcgatc tggtcttaat cggtccgcgg
     1441 ccggagccgc tcgagatcat ccacgacctt ccagaacacg ccggccgcta ccccaccctg
     1501 ctgcgcgtca agccggggtt ctttgtcgca ggcccgattt aacgcaagat cgaccctgcg
     1561 ggcgatgacg tgcaagtaag tttcctgccc tatttattct gctgaatcgc gcaagttttt
     1621 tgtaggggtg aacgtttacg ttcaccctgt gatgtattgt tagaccgaaa atcagaactc
     1681 ctcaaaaccc tgaaaggcgg gcgcatcggc cggttcgacc cattgggaag atgcaccccc
     1741 acgcctggct gacctgattt tgagtttttc acaacaatgg gtcggtcagg cagaaaagag
     1801 gcttgacaat caattttgaa agcggtagct tattaccgac aatgacagta gatggtggag
     1861 gtcaaggggg aattctggaa agaaccgaaa aaaccccttg acaaactgaa aagggctcgt
     1921 atactaacac gttcgccggg cagaagtatc gacccggcag ggcagagggg tgtctctgcc
     1981 gtaacgtctg ttctttgaaa acttcataaa gcgtgacgtg tacaagccag cacgtctttt
     2041 gtgggcctta cggaattttg cggtaaggcg attcagaaca acacgagcgt ttttggacgc
     2101 tctttgcacg tgaaataatt tttcacttgt ggagagtttg atcctggctc agaacgaacg
     2161 ctggcggcgt gcctcataca tgcaagtcga gcgaaaaggt ccccgcaagg ggacccgaaa
     2221 gcggcaaacg ggtgcgtaac acgggggtaa tctgccctgc gacgggggat aagccttcga
     2281 aaggaggtct aataccgcgt acgttccttg ggtcgcctga cccggggaag aaaggtcccg
     2341 cagcaatgcg gggtcgtcgc aggaggagcc cgcggccgat taggtagttg gcgggataac
     2401 agcccaccaa gcctgcgatc ggtagccgac ctgagagggt gtccggccac actgggactg
     2461 agatacggcc cagactccta cgggaggcag cagtagggaa tattgcgcaa tgggggaaac
     2521 cctgacgcag caacgccgcg tggaggatga agtccttcgg gatgtaaact cctgtcagga
     2581 gggacgaatc tctcccttac gggagactga cggtacctcc agaggaagtt ccggccaact
     2641 ccgtgccagc agccgcggta atacgggggg aacgagcgtt gttcggaatc actgggcgta
     2701 aagggtcggt aggcgggacg gcaggtcggc ggtgaaagct ctccgctcaa cggagaatcc
     2761 gccgccgaaa ccgctgttct tgaggacgga agagggaagt ggaattcctg gtgtagcggt
     2821 gaaatgcgta gatatcagga ggaacaccgg tggcgaaggc ggcttcctgg tccgtacctg
     2881 acgctgagcg acgaaggctg ggggagcaaa caggattaga taccctggta gtcccagcgg
     2941 taaacgatga gcacttggtg ttggacccgt cgaccgggtt cagtgccgca gctaacgcag
     3001 taagtgctcc gcctggggag tacggccgca aggttgaaac tcaaaggaat tgacgggggc
     3061 ccgcacaagc ggtggagtat gtggtttaat tcgacgcaac gcgaagaacc ttacctgggc
     3121 ttgacatgct ggtggtaggg agtcgaaaga cgaacgaccc cgtaagggga gccagcacag
     3181 gtgctgcatg gctgtcgtca gctcgtgccg tgaggtgttg ggttaagtcc cgcaacgagc
     3241 gcaacccctg tctgcagttg ccagccccgc gcaagcgggg ggaactctgc ggagactgcc
     3301 cgggatgacc gggaggaagg tggggatgac gtcaagtcct catggccctt acgtccaggg
     3361 ctacacacgt actacaatgg ccgttcacaa tgggaggcaa gaccgcaagg tggagcagat
     3421 ccccaaaagc ggcctcagtt cagattgtcg tctgcaactc gacggcatga aggtggaatc
     3481 gctagtaatc gcggatcagc aggtcgcggt gaatacgttc ccgggccttg tacacaccgc
     3541 ccgtcaagcc atggaagccg ggagtacccg aagtcaccta tcgaacccgc aaggggcgag
     3601 ggtgccgaag gtaataccgg tgactggggc taagtcgtaa caaggtagcc gtagcggaag
     3661 ctgcggctgg atcacctcct ttctaaggag acctgaaaaa caggtgtgac tctccccata
     3721 cggggagact ccaatggtcg acacagaaga tgggcggctt gtgcacgtcg cttttaatgt
     3781 cagtggattc ggaagactgg cacattggtc aagcagggtc ccgttggtgg atctgaaaga
     3841 gaaagtaccg gtcatccgta cacctatagc tcagtcggtt agagcgtccc gcctctggcg
     3901 ggaaggtcaa cggttgaccg aaacgggaat gagtactgcg gacaaggaaa acgtgggcct
     3961 atagctcagt cggttagagc gcgcgcctga taagcgcgag gtcagtggtc cgattccact
     4021 taggcccacc agggaaaaca gggatcaggg attagagaag agagatcagg aagtgtggac
     4081 ccagaccgaa gggaggtgtg accagggacc gtaacgagtc gagttggtaa gcgtttggag
     4141 gtcacagggg tgccatcgcc aagctggccc tgccgagggc aggccagcgc tggaggtaag
     4201 caagcgaggc acaaaggaat aaccttcaga ggagggaaag taacggtgag aaccgcgaaa
     4261 tcgtttttgt ggctggttgc gttgggtgta gttctcagtt gtgcggcagt ggtctggggc
     4321 acggaatccc gcatcgccac ggtgggtaac aatccactgt tcctgcggga cgacatcggc
     4381 atgttcacct ttccatccac ggccgtcgat taccgcaagc acatcttcgc cgagcattta
     4441 accaacgggg gcagcggtga gatcggtgta gatcagcggg atttcttcgg cgggccgatt
     4501 tcgggacggg tcgggatagt caccaatttc gtcggtcaat ctgccctggg ggtattcatt
     4561 ggacagcagg aggagcactt ccggggtcct ctgtttccgg tggatcaacc ccaaacccgg
     4621 ttcaacctga tttacgggaa ggaaatgtcg aaggtgacgc tcgggttgaa cttcaatttc
     4681 tcgggtgtaa aaaccgagag ccgggttggc aatgtgccca cgggcaccct gcttcaggac
     4741 aaggcgggga cattcggttt cggtgccggg ttgggcgttc cgctgaccga aacgcaggat
     4801 ctccaggtgg ccggtcacat cgaactgcgg aacttcagct ccgacacggg gaacgtgacc
     4861 gtgagacagg acgacggcaa ctggagcctc ctgttcctgg gacgtgactt cattgaatac
     4921 aacgacatgg taacgctcgt gccggcgggt caggtggaaa tcttcaacga gaagagccgg
     4981 agggtgcccg gtccccagcc cgatacagct cacgagatca aagggacgaa ctggcagttc
     5041 gggttggggt gcaacgtaca gccgaacgaa aaagtcctga tcaactcggc cgtgggggtc
     5101 cgcggcggct cctctcagca caaaattgac cagaatctca ccgaagcagg taccagcaaa
     5161 accagcttct tcaccatccc gtttgctctt ctgggcatgg atgtggaagt gaagaaaggg
     5221 ctggacttcc gggcgggtgc taccaaagag atccggagcg acaagaacaa catcctgaac
     5281 accaagaaca ccgacaccga tttcacctac gccattgggg cggcggtgcg gctgggtgac
     5341 gtaaccttcg acacccgggt ggccaatacg ttcttgaaca acgggccgta cttgctctcc
     5401 ggccagacga ccttcggcgg gatgttcccg caggtttcgt tcacctacgc ctggaagtag
     5461 ttcagcgcag ttcgttcgta cggtttttgc cggtggctct gtcgctgaac agagccaccg
     5521 gtcatcttga gcggcgccag ttttggaccg cataccagag gcgaaaagtt gcaccgctgg
     5581 tctctggtcg cgaaagtatg ttggggatat agctcaattg ggagagcgcc tggtttgcaa
     5641 ccaggaggtt accggttcga tcccggttat ctccataaga aagcagttga caaccagagg
     5701 gattggctct tataatagca tttccctgcc caatcacgta cctatagacg gagcagggat
     5761 ggagtgtcgc aaacattccg gctgaaggcg aaaaaccttc agcgacggct ctttgacaag
     5821 ttcataccgc gggtaatgtc tgtggcagtt ctccccgtac gggaagggcg gctgcagatt
     5881 tttgggcaag catctgttgc caaaagcaca gatccgagca ctttggatca agttccaaag
     5941 ggcacatggt ggatgccttg gcacacgacg gcgacgaagg acgtggtaag ctgcgataag
     6001 ctccgctgag tggcacgcac acagtgaggc ggagatctcc gaatggggca accccgcccg
     6061 ggtcatgccg ggcgatcctc ccgtagggga ggaggcaaac gcagggaagt gaaacatctc
     6121 agtacctgca ggaaaagaaa gcaattgcga ttcccctagt agcggcgagc gaacggggag
     6181 gagcccaaac cggccggcac gcagaagcct gcacgcgttg tgccgccggg gtagcggggc
     6241 cgtggaatcc ggccgggtgc agtccggccg caggagttac aaacgtcggc ggtagccgaa
     6301 gttgcgtgga aaagcaaacc agagacggtg acagtccggt aggcgaaact tccgacgctc
     6361 ctgttgccac ggttcccaag tatcgcggga cacgtgcaat cccgcgtgaa tccgtcccga
     6421 ccacgggata aggctacata cgtgcgtgtg accgatagtg aaccagtacc gcgagggaaa
     6481 ggtgaaaagc acccctgacg gggagtgaaa agtacctgaa accatgtgct tacaagcggt
     6541 gggaggcgat tttccgcctt cgggcgggag agagctgacc gcgtgcctat tgaataatga
     6601 tccggcgagt ttcttgtctg ctgaaaaggt taaggccaaa acggccgtag ccggagcgaa
     6661 agcgagtctg aacagggcgc gttccgcgca agcggaacag gtagcaggca gaagacccga
     6721 agccaggtga tctagggatg gtcaggttga aatcccggta acacgggatg gaggaccgaa
     6781 cccacttgcg ttgaaaagcg aggggatgaa ctgtccctag gggtgaaagg ctaatcaaac
     6841 ctggcgatag ctggttctcc ccgaaatata ttgaggtata gcctcgccgc aaagactggc
     6901 ggaggtagag cactggatcg gcaaggggcc ttaccgggct accgacccga accaaactcc
     6961 gaatgccgcc agttcgttcg gcgggagaca ggcagtgggg gataagcttc attgccaaaa
     7021 gggaaacaac ccagaccgcc agctaaggtc cccaagtgtt ggctcagtga ctaaggatgt
     7081 ggaggtacgc aaacaaccag gatgttggct tagaagcagc catcatttaa agagtgcgta
     7141 actgctcact ggtcgagtgg ctccgcgccg ataattcccg ggactaagcc agccaccgaa
     7201 gctgcggact ccgatagcaa tatcggagtg gtaggggagc attccgccgt agagtgaagg
     7261 tgtcgcgaca agcggcgctg gacgaagcgg aagcgattat gccggaacga gtagcgataa
     7321 aacgggcgag aaacccgttc accgaaagcc ccagggttcc tgaggaaggt taatccgctc
     7381 agggtcagtc ggtccctaaa ccgaggccga aaggcgtagg cgatggaaaa caggcgaata
     7441 ttcctgtacc accgacacgg cgctatccag cgaaggggtg acgcagaagt gaccgccagt
     7501 cgcctgatgg aaatggcgat ctaagcgcgt aggtgcggga ggcaggcaaa tccatctccc
     7561 ggtcatcacc gagacgcgat ggggaggccg caaggccgca aactggcgat aaccacgctg
     7621 ccaagaaaaa cctcgtagcg agccgtgtgg gtgaccgtac cgcaatccga cacaggtggg
     7681 cgaggagaag atcctaaggt gctcgagcga accctcgtta aggaactagg caaaatggcc
     7741 ccgtaacttc ggaagaaggg gcgcccgttg taggtgaatc cgtacaggag gagccgaagc
     7801 gggccacagt aaagaggcct gggtgactgt ttactaaaaa cacatgtctc tgcgaagccg
     7861 ccaaggcgac gtatatggac tgacacctgc ccggtgccgg aaggttaaag ggaggggtta
     7921 gtggccgcaa ggtcgcaacg ctctgaactg aagccccggt aaacggcggc cgtaactata
     7981 acggtcctaa ggtagcgaaa ttcctcgtcg ggtaaattcc gacctgcacg aatggtgtaa
     8041 caacttgggc gctgtctcaa cgaggggctc ggcgaaactg tagctacggt gaagatgccg
     8101 tatacccgca tcgggacgga aagaccccgt gaacctttac tgtaccctgg tattgggttt
     8161 tggtcatgca tgtgtaggat aggtgggagg cggtgaaggc ccgccgccag gtgggctgga
     8221 gccaacggtg aaataccacc cttgtgttat cggaattcta accctggtcc ttacacgatc
     8281 gggaacagtg ccaggcgggc agtttgactg gggcggt
//
LOCUS       DBNGZA010000033         7726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_854451, whole genome shotgun sequence.
ACCESSION   DBNGZA010000033 DBNGZA010000000
VERSION     DBNGZA010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_854451"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC16FU_00675"
     CDS             complement(<1..265)
                     /locus_tag="AC16FU_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR2992924.1"
                     /translation="MGRESVLVKDRSRNAPEESRLLRAVRNILSKSDAKRSDHLIHER
                     IRLGILSALAVNVSLSFSDLKKLLNTTDGNLSVHARKLEEAGYL"
     gene            complement(258..854)
                     /locus_tag="AC16FU_00680"
     CDS             complement(258..854)
                     /locus_tag="AC16FU_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992925.1"
                     /translation="MPLNSADPPDVRAQAAEDLRFIRETLAKAGSFTAVPGWGMVAMG
                     ISALAATVVAAARSSSAEWLVVWLVEALLAISVALLSLRRKAQRANVPVFSGPAARFL
                     LSLCPPLFAGALLTAVFYRFDLVTLLPSLWLLLYGTGIVTGGAFSIRIVPILGLCFML
                     LGVAALFTPTSWGNFFMGAGFGGLHVVFGTIIARRHGG"
     gene            complement(995..1456)
                     /locus_tag="AC16FU_00685"
     CDS             complement(995..1456)
                     /locus_tag="AC16FU_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992926.1"
                     /translation="MAKSPEFSLEAAHKFFSAECFNKAWELIEKPHRTPEENEQMIRL
                     NHASLWHWTQRPDCTKRNLSIGYWQAARIYTVLGQGDNARWYGRLCLEVSVAEPPFYR
                     AYAFEALARAETIAGNKQNAREYLAQARDLAKQVSEAEERKALLADLAAID"
     gene            complement(1517..2332)
                     /locus_tag="AC16FU_00690"
     CDS             complement(1517..2332)
                     /locus_tag="AC16FU_00690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2992927.1"
                     /translation="MSHDKDYVLGTHDEEIARLALQHRVWRPRALDAWRRAGFTVGQT
                     LLDIGCGPGHASIDLAEIVGPSGRVIAIDRSRRFLDALDAARRQRGLNNIEALELDLD
                     EAELPALGADGAWSRWVFAFVKHPRELLARVAGALKPGGVLVLHEYFDYSTWRLAPRL
                     PELEEFVKVVIESWRADGGEPNIGLDLPMWLAELGFEIKTLNPIIDVVPASNFIWQWP
                     KAFIQVGLRRLVDLGHLTSERAQAIAEAFAAAETAPHTLMITPAVLEIIAVRK"
     gene            complement(2371..2913)
                     /locus_tag="AC16FU_00695"
     CDS             complement(2371..2913)
                     /locus_tag="AC16FU_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR2992928.1"
                     /translation="MAFLRGINVGGRKIIKMEDLSRMFVSMGFRNVKTYIQSGNVIFE
                     TAEINSNVLTKKIEKELHKSLGYEVRVFLRTILEVEDIVQHNPFKGMAANANVGMYVT
                     FLSEEPKSKPELPLVSPQKDVEVFRIRKREAFILCRKIKGRSGFPNNFIEKELGMPAT
                     TRNWTTISKIIIQTLPKPGR"
     gene            complement(2953..3423)
                     /locus_tag="AC16FU_00700"
     CDS             complement(2953..3423)
                     /locus_tag="AC16FU_00700"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR2992929.1"
                     /translation="MQTIDVTEEHVRQILKDLVFRALWFPLFVFLLHVFLSQVLRAYQ
                     KFPVLGSPMHLLGGIAITFFFSRVLTILQKSGLAGELDRRIRTMLLFALTATAAVFWE
                     FGEFLVDHFFGTRMQLGLEDTLLDMFLGIVGGVSYLSAGLLVSSAGRNKRIART"
     gene            complement(3624..4319)
                     /locus_tag="AC16FU_00705"
     CDS             complement(3624..4319)
                     /locus_tag="AC16FU_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR2992930.1"
                     /translation="MRYKAKRALARASFQRVFWLQLFAIAIFGGCTSKRSESPNKTFS
                     KAEAIGDSMFPSEFKFSTSKYIYSIKGDGQGLRKVNETDTKAFVLVQESQDMLSHLSF
                     LDRGEGVILLFEITNDSLDAGWGEIVEFNQSSLKIYWRKNIPFNVGEPLVEDNYVYLT
                     GIGYIAKLALDSGKVIWQHDNLYDPKSYHFNSFEIPKIAGDTVLFMESPDQVKEAKTV
                     RVHRIAGKILSIK"
     gene            complement(4364..4684)
                     /locus_tag="AC16FU_00710"
     CDS             complement(4364..4684)
                     /locus_tag="AC16FU_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2992931.1"
                     /translation="MGAKSKGKTVLEQDSSWSVYILRCTDGSYYVGVASDVENRLHEH
                     NAGQAASFTRERRPLELVYVEECGDYVTAWRRERQIKGWRREKKERLINGFPSTRPKD
                     SLRA"
     gene            complement(4724..4813)
                     /locus_tag="AC16FU_00715"
     tRNA            complement(4724..4813)
                     /locus_tag="AC16FU_00715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4776..4778),aa:Ser,seq:gct)
     gene            complement(4850..5137)
                     /locus_tag="AC16FU_00720"
     CDS             complement(4850..5137)
                     /locus_tag="AC16FU_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2992932.1"
                     /translation="MYYVYILRFVGTGRFYIGQTQNLERRLLKHQQGKTKSVKNRGQF
                     EVIFVDRFATRAQAMRREKQIKSYKGGQAFEKLLQARTSLAKLVEVPPLAG"
     gene            complement(5419..>7726)
                     /locus_tag="AC16FU_00725"
     CDS             complement(5419..>7726)
                     /locus_tag="AC16FU_00725"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2992933.1"
                     /translation="GGYINAFTRKELTGFLTLVPGEEFDFALDLLDEMLFHSIFPDEE
                     LAKERKVVIEEIRKDEDNPDYEVERFFDSQVYAGTPYARPVLGYPRYIEQVAREEIIS
                     YWQRRYVPGNMVAVVVGDFEPEEMLKQLQSVFGSEPAQPVSPETAVRWLLPKEVRVLR
                     KQLDVQTTYLNFAFAAPHFSDPDYIPFSFLTEILNSGEASPLNQALTSGSDPLALTCT
                     AALETQKEFSALTLSVTTDRAEKADSIIGAVKQYFSLPGGFSFTADQIQATLVTLETG
                     EEFLLEKPHYYGLMRAPFYVTTGYAFLESYWDRMAKVTPRDLQRVAQKYFNPPVFTTT
                     VVTPIQKAPKGTSEQSSTSLQRKLLPNGLEVIIKSNPDSKVFAFTLLGKYRSAQERDD
                     QAGMTDFIHRMLARGTQSRSKEAIASVLRAMGAELTTNDNPAIPYDDIYTTRAYSFIK
                     LQTVDRFADSALALVADLVLHPLFAAGEIAKTKREVFDLLRREEQSPTQAARNLFYRT
                     IFAGGPYAKTVMGDSATVAGFTPDEIAAYHRTYFSGGNLILAVATGLPATSVLEKIKT
                     FFASLPTLSRELPQVIRPEAPAELHEVEKPLKKEQISICVGTLLPGANDPDIPALQVM
                     NAILSARLGLELREKQGLAYSVGSSVTFDRNFGWLMIQLGTGKGNFALAQKGISGEIE
                     KLQNGGVGDDELQKARKSLWGNYLLANLSRLNQTYWMAVNQFIGRGFDYDQKFVARLE
                     AVTTKDVQRAAQQYLNLKHAVLTTAGMR"
BASE COUNT         1746 a         2159 c         2001 g         1820 t
ORIGIN      
        1 ggaggtatcc cgcctcctcc aatttgcgcg catggacgct taaattgccg tcggtcgtgt
       61 tcaagagttt tttcagatcg ctgaaactga gcgacacatt gacggccaaa gcgctcaaaa
      121 tccccagccg tatccgctcg tgaatgaggt ggtcggatcg ttttgcgtcg ctttttgaga
      181 gaatattgcg taccgcgcgc aaaagccgcg actcctcagg ggcatttcgg gaacggtctt
      241 tcaccagaac gctctcccta cccaccgtgc ctccgggcaa tgatggtgcc gaaaacgacg
      301 tgcaggccgc cgaacccggc gcccatgaag aaattccccc acgaggtcgg agtgaagagc
      361 gcagccacgc ccaggagcat gaagcacagc cccaaaatcg gaacgatccg aatggagaac
      421 gcgccgccgg tgacgatgcc cgtgccgtag agcaaaagcc acaggctggg cagtaaagtc
      481 acgagatcaa aacggtaaaa aaccgcggtg aggagagccc cggcgaaaag cggcgggcaa
      541 agactcaata agaacctcgc cgccggcccg gaaaataccg ggacattcgc tcgttgcgct
      601 ttccggcgca gagacaagag cgcgacggaa atcgccagta gggcttcgac cagccagacg
      661 acaagccatt ctgcggaact ggagcgagcc gccgccacca ccgttgccgc cagggccgaa
      721 attcccatgg caaccatccc ccaacccgga acagccgtga acgaccccgc ttttgccagc
      781 gtttcgcgga tgaaacgcag gtcttcggcg gcttgcgccc ggacatcggg cgggtctgca
      841 gagttcagag gcacagggcg atcatcaaac atgtattact acatacggca caatttcaat
      901 ttagtcaagt actttatgat aaaaaatata aaccgattct ttttcgtgct gccagtgcat
      961 aggcagacgc accggcgcgt gtgcctggga tgcgtcagtc gatggctgcc aggtcggcca
     1021 ggagcgcttt cctctcctcg gcctctgaaa cttgtttagc gagatccctg gcttgagcca
     1081 aatactcccg tgcgttttgt ttatttccgg ctattgtttc cgcgcgggct aacgcttcga
     1141 aagcgtaggc gcggtagaaa gggggttcag caacgcttac ttcaaggcag aggcggccat
     1201 accagcgggc attgtcgccc tgaccaagca cggtataaat ccgcgctgcc tgccagtagc
     1261 cgattgacag gtttctcttg gtgcagtcgg gtctctgggt ccaatgccac agcgaggcgt
     1321 ggttcaaccg gatcatttgc tcgttttctt cgggcgtgcg gtggggcttc tctatcaatt
     1381 cccacgcttt gttgaagcat tcggcggaaa agaacttgtg cgcggcctct aaactgaatt
     1441 caggggactt agccatcgtt gcctcctaaa ggtgcatctg atgaagttgt gacaagaggt
     1501 gttcaaatcg tccgtctcac ttacgaacgg cgatgatctc cagcaccgcg ggcgtaatca
     1561 taagcgtgtg cggcgcagtc tcggcggctg caaacgcttc cgcgattgcc tgcgcgcgtt
     1621 cactggtcag atggcccaga tcaaccaggc ggcgcaggcc cacttgaatg aacgcctttg
     1681 gccactgcca aataaagtta gatgccggaa cgacgtcgat tatggggttg agagtcttaa
     1741 tctcgaatcc cagttctgcg agccacatgg gcaggtcgag cccgatgttc ggctcacccc
     1801 catcggcgcg ccagctctct atcaccacct tcacgaactc ctcaagctcc ggcaaccgtg
     1861 gcgcaagccg ccaggtggag tagtcgaaat actcgtgcag aaccagtacg ccgcccggtt
     1921 tcaatgcgcc tgccacccgg gcgagaagct cgcggggatg cttgacgaac gcaaagaccc
     1981 agcgggacca ggcgccgtcg gcgccaagag cgggcaattc cgcttcgtcg aggtcgagtt
     2041 caagcgcttc gatattatta agtccgcgtt gccgtcgtgc agcatcgagc gcgtccagaa
     2101 accgtctcga cctgtcgatg gcgatgaccc tcccggaagg tccgacgatc tctgctaaat
     2161 caatcgaggc gtgaccgggg ccgcacccaa tatcgaggag cgtttggccc accgtgaatc
     2221 cggcccgccg ccaggcatcc agcgcgcgcg gccgccagac gcgatgctgc agcgccaacc
     2281 gtgcgatctc ctcgtcgtgc gtgcccagga cgtagtcttt gtcgtggctc atcgttgctc
     2341 tcttctccgc agcggttcgt gaactactca ctacctcccg ggtttcggca gagtttgtat
     2401 tatgatttta ctgattgttg tccagttccg tgtcgttgcc ggcattccaa gttctttctc
     2461 gatgaagttg ttcggaaaac cggaccttcc ctttatcttg cggcataaaa tgaacgcttc
     2521 acgttttctt atgcggaaaa cctcaacgtc tttttgagga gataccaaag ggagttccgg
     2581 cttgcttttt ggttcttcgg ataaaaaagt gacgtacatc cctacattgg cgttggctgc
     2641 cattcctttg aaaggattgt gttgcacaat gtcttcaact tcaagaatcg tcctcaagaa
     2701 aactcttacc tcataaccca gcgatttatg gagttctttc tcaattttct tggtaagaac
     2761 gttggaattg atttccgcag tttcgaaaat gacgttcccg ctctggatgt aggttttcac
     2821 gtttctgaat cccatcgata caaacatccg gctcaagtct tccattttta ttatcttgcg
     2881 gccgccgaca ttaatgccgc gcagaaatgc cacatatgta atcattttta tcgcctatgc
     2941 cccttttcta ttttacgttc gggctatccg cttgtttctc ccggctgacg acaccaacag
     3001 ccccgctgat aagtaagaga ccccgcccac aatgccgagg aacatgtcga gtaaagtgtc
     3061 ttccagacca agctgcatac gagtaccgaa aaagtgatcc accagaaact cgccgaattc
     3121 ccagaacacc gccgccgtcg ccgttaaggc gaagaggagc atggttcgga tgcgccgatc
     3181 gagttcccct gccagaccgc tcttctgcag aatcgttagt acacgggaga aaaagaaggt
     3241 gatggcaatg ccgcccaaaa ggtgcatcgg ggaaccgagg acagggaatt tttgatacgc
     3301 gcggagtacc tgcgaaagga aaacgtggag gagaaagaca aagagtggga accagagggc
     3361 acgaaaaacc aggtctttta aaatttgtcg aacgtgctct tcagtgacat ctatcgtctg
     3421 caaggtatcc tgtcctcgac cctaattcga aaccaaaact cggatgcaca gggctgatct
     3481 gcctgacttg tatttaggcc gaccggccta ccaccatgcc atcgatgtat attatgaacg
     3541 ggtaggtaga atgtaaacgc tgttcatttt tttaaatgag ctaggaatct gggacaactt
     3601 tagaacttac tttcaaactg ccatcatttt atagatagta ttttaccagc tatgcgatgg
     3661 actcttaccg tttttgcttc ctttacttgg tcagggcttt ccataaaaag tactgtatcg
     3721 cccgctatct tcggaatttc aaatgaattg aaatggtagc tttttgggtc gtaaagatta
     3781 tcatgttgcc aaataacctt ccccgagtca agcgccaact tagcaatata ccctatacca
     3841 gtaaggtata cataattgtc ttccacgaga ggttcaccaa cgttgaaggg gatgtttttt
     3901 ctccagtata tttttaaaga gctctgattg aactctacaa tttcacccca tccagcgtct
     3961 aatgaatcat tggtaatctc gaataaaagg ataacgccct cgcccctgtc aagaaaagaa
     4021 agatggctga gcatatcttg gctttcttga acgaggacaa atgcttttgt atcagtttcg
     4081 tttactttcc tcagaccttg accgtcacct ttaattgaat aaatatactt ggaagtggaa
     4141 aatttgaatt cggacggaaa catcgagtcc ccaatagctt ctgccttact aaaagtctta
     4201 ttgggtgatt cggagcgttt cgaagtacag cccccaaaaa tggcaattgc gaataactgc
     4261 aaccaaaaaa ctcgttgaaa ggaggctcgc gctaatgccc ttttggcttt ataccgcatt
     4321 tgcacctcgt tatcgaatca agcggagagg ctgggattcg ccgtcaggcc ctgagcgagt
     4381 cctttggacg agtcgaaggg aacccattta tgagtcgttc ctttttctca cgacgccatc
     4441 ctttgatctg cctctctcgc ctccatgcgg tcacgtaatc gccacattct tcaacataca
     4501 caagctccaa cggtcgtcgc tccctcgtaa aagatgcggc ttgacctgcg ttatgttcgt
     4561 ggagacgatt ctcgacatcg cttgcgacgc ccacgtaata ggaaccatct gtgcagcgta
     4621 agatgtacac agaccaggat gaatcttgct ccaacacggt tttacccttc gacttcgctc
     4681 ccatcgggtc tgagaccctc agggtcgatg acagggtttt caacggagag gctgggattc
     4741 gaacccaggg tcccgatttc tcggaacaac tgcttagcag gcagctgcct tcagccagct
     4801 cggccacctc tcctaaaatc atccgccatt tcgctccacc accatctgct tatcccgcca
     4861 gaggcgggac ttcaaccagc ttggccaatg atgttcgagc ttgtaacaac ttctcaaatg
     4921 cttgacctcc tttgtagctc ttgatctgct tttctcttct catggcctga gcccgggttg
     4981 caaatctgtc cacaaatatt acctcaaact gccctcgatt cttaaccgac tttgtctttc
     5041 cctgttggtg cttaagtaaa cgcctctcta agttttgtgt ctgcccgatg tagaatcgtc
     5101 ccgtccctac gaagcgcagg atgtatacgt agtacatgtt ttcagataac cagagaggtt
     5161 gggattcgaa cccagggtcc cgatttctcg gaacaactgc ttatcccgcc gcaggcggga
     5221 cttaagccaa ctcggccacc tctgcttagt tcaaggtttt ctcaaccgac accaactcca
     5281 actgcttatc tcccgctttt ggcgggacgg gacttcagtc ccgcaaaatg cgggaccacc
     5341 tctccagatt cggcggacaa aaaagcttcg cccgatccaa aagtacgttg tccggctcac
     5401 tcctccaaat aaccctcctc accgcatccc cgctgttgtc aaaacggcgt gtttcaagtt
     5461 gagatactgt tgcgctgccc gctggacgtc tttcgtagtc acggcctcca ggcgcgcgac
     5521 gaacttctga tcgtagtcaa atccccgacc gatgaattga ttcaccgcca tccagtaggt
     5581 ttgattcaga cgcgacagat tggccaatag gtagtttccc cagagtgatt tcctggcttt
     5641 ctgcaattcg tcatcgccga cgcccccgtt ttgcaacttt tcgatttcgc cgctaattcc
     5701 tttctgcgcc agagcgaagt tccccttgcc cgttcccaac tggatcataa gccagccgaa
     5761 gttgcggtcg aaagtgaccg acgaccccac cgaataggcc agcccctgct tttcccgcag
     5821 ttccagcccc agccgggccg agaggatggc attcatcacc tggagtgccg ggatgtcggg
     5881 atcatttgcg cccggcaaaa gcgtcccgac gcagatggag atttgttctt tcttcagcgg
     5941 cttttccacc tcgtgcaatt ctgccggcgc ctccggccgg ataacctgcg gcagttcacg
     6001 cgacagggtt gggagtgagg caaagaaggt tttaatcttc tccagtaccg acgtggctgg
     6061 cagcccagtc gctaccgcca ggatcagatt tccgccggaa aagtaggtgc ggtggtaagc
     6121 agcgatctcg tccggtgtga acccggccac cgtggcggaa tcccccatca ctgtcttggc
     6181 ataggggccg ccggcaaaaa tcgttcggta gaagagattg cgggcggcct gggtcggcga
     6241 ctgctcctct cggcgcagga ggtcaaagac ctcgcgtttg gttttagcga tttcgccggc
     6301 ggcgaaaagc gggtgcaaga cgagatcggc caccagtgcc aacgccgaat cggcgaaccg
     6361 atcgaccgtt tggagcttga tgaaggaata ggcgcgggtg gtgtaaatgt cgtcgtaggg
     6421 aatggcggga ttgtcgttcg tcgtcaactc cgctcccatc gcccgcagga cggaagcgat
     6481 tgcttctttg gaacgtgatt gcgtcccgcg cgccagcatc cggtgaatga aatcggtcat
     6541 cccggcctga tcatctcgtt cctgggccga ccggtacttc cccagaagag tgaaggcaaa
     6601 gaccttggaa tcggggttgc ttttgataat gacttccaga ccgttcggga gcagtttgcg
     6661 ttgcaaggag gtgctggatt gttccgacgt cccctttgga gctttctgga ttggtgtcac
     6721 caccgtcgta gtgaaaaccg gcgggttgaa atacttctgc gccacgcgct ggagatcccg
     6781 cggtgtcacc tttgccatcc tgtcccaata cgactccagg aatgcgtagc cggtggtcac
     6841 gtagaacggc gcccgcatca ggccgtagta gtgcggtttt tccaggagga actcttcccc
     6901 ggtttccaaa gtcacgagcg tcgcttggat ttggtccgcg gtgaaggaga aaccccccgg
     6961 aagagaaaag tactgcttca ccgcaccgat tatggaatcg gctttttccg cccggtcggt
     7021 ggtgaccgaa agggtcagtg ccgaaaattc tttttgtgtt tccagtgccg ctgtacaggt
     7081 gagcgcgagc ggatcggaac ccgaggtcag tgcttggttc aacggcgagg cctctcccga
     7141 gttcaagatt tcggttaaaa aactaaaggg aatgtaatcg gggtctgaaa aatgaggcgc
     7201 cgcaaaagca aaattgaggt aagtcgtctg cacgtcgagt tgcttccgga ggacccgaac
     7261 ttccttcggt aacaaccagc gtaccgcagt ctcgggcgac accggttgcg ccggctccga
     7321 gccaaaaact gattgaagtt gtttcagcat ttcctcaggc tcaaaatccc ccaccaccac
     7381 cgccaccata ttgccgggaa cgtagcggcg ttgccagtag ctgataattt cttcccgggc
     7441 aacctgttcg atgtagcgcg ggtaccccag aaccggccgg gcgtagggcg tcccggcata
     7501 gacctgggag tcgaagaacc gctccacctc gtagtcggga ttatcctcat ccttgcggat
     7561 ttcctcgatg accaccttcc gctctttggc cagttcctca tcggggaaga ttgagtggaa
     7621 gagcatctcg tcgagcagat ccagtgcaaa atcgaattct tctccgggga cgagcgtcag
     7681 gaatccggtc agttccttgc gggtaaaggc attgatgtaa ccgccg
//
LOCUS       DBNGZA010000034         3575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1172510, whole genome shotgun sequence.
ACCESSION   DBNGZA010000034 DBNGZA010000000
VERSION     DBNGZA010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1172510"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC16FU_00730"
     CDS             complement(<1..336)
                     /locus_tag="AC16FU_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR2992934.1"
                     /translation="MKIGLIAMSGVRAHNEELTQLGLTLPGFVERNKIIASLPSLSLL
                     TLAALTPKKFAVEYHEIADLKKESTLPDDYDLVAISSFTAQMYEAYEVADRYRRKNIP
                     VVLGGLHVSS"
     gene            complement(333..716)
                     /locus_tag="AC16FU_00735"
     CDS             complement(333..716)
                     /locus_tag="AC16FU_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992935.1"
                     /translation="MIKINFILAWLWIVVGFVTGFLLGLNFHREEWLGGYTSLKRRLY
                     RLGHISFFGLAILNLLFYFTTQQILPPNRSIDVASWGFVVGAVAMPACCFIMAHKPRL
                     RAIFLIPVLSLTLAGVLTLWEVIGR"
     gene            complement(741..2057)
                     /locus_tag="AC16FU_00740"
     CDS             complement(741..2057)
                     /locus_tag="AC16FU_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR2992936.1"
                     /translation="MSWLDDYRRKIGSPEEAVAFVKSRERIYISGNAATPFVLLDALA
                     ARKDDLHDVEVIHVLLLGKGREDPLSRPGMEGHFRHNSLFVGPSDRAAVQTGRADYIP
                     VFLSEIPRLLRTQLPIDVALIQTSPPDEHGFLSLGVECLSTRAALESARVVVAQVNEK
                     MPRTLGDSFVHFSKLAKIVEVAEDLPELLPEPPGAIEEKIGRFVGELVEDGATLQAGI
                     GGIPDAVLQQLRDRKDLGVHTEMVSDGLMELMESGVITGAKKTLHPGKVVATFLLGSR
                     RLYDFAHNNPAFEFHPADYTNDPFLIAQNDKMVAVNSAIEVDLTGQVCSDSIGYQIYS
                     GFGGQLDFVRGAARSKGGKAVIALPSTAKGQSFSRIVPALREGAGVVTTRGDVHYIVT
                     EYGVAYLHGKNLRERLRALIEIAHPKFREDLERSHNQRRVREMSPV"
     gene            complement(2054..3172)
                     /locus_tag="AC16FU_00745"
     CDS             complement(2054..3172)
                     /locus_tag="AC16FU_00745"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR2992937.1"
                     /translation="MIVGVPRERPPGRGMEERRVGLSPAGVAELVKAGARVVVEREAG
                     TGAHFADATYEKAGAQIVLSHEEAFQRADIICKVGRVADDELPLLHEGQILTGFLHLI
                     TAPKSTLQTLLDKKITATGYELIEEADGRLPVLSPTSEIAGKMVPQLAGRLLERGRGT
                     LLGGIPGIPPADVVILGGGTLGYHAARALRGIGAMVYLLEKHPRRLEELDKYFAGSLV
                     TAVANRVNIEKFVPFADVLIGAVLEPGARAPILVTREMVRTMPRGAVILDFSIDNGGC
                     IETSRPTPSEEYVYQEEGVWHYCMPNASSLVARTASPALTNAALPYLKSLVERGVRES
                     LRGMTDLAKGVSAHAGFVTQEHLAREYAFEYRPLERLL"
     gene            complement(3428..3556)
                     /locus_tag="AC16FU_00750"
     CDS             complement(3428..3556)
                     /locus_tag="AC16FU_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HIR2992938.1"
                     /translation="MGRYALRFEWSDGHSTGIYTFDYLRSLCPCEKCRAKAPPKAG"
BASE COUNT          718 a         1060 c         1102 g          695 t
ORIGIN      
        1 cgaagagacg tgcaagccgc ccagcaccac cgggatgttt ttgcggcgat accggtcggc
       61 cacctcgtag gcctcgtaca tctgcgcagt gaaggaactg atggcgacca agtcgtagtc
      121 gtccggcagt gtggactctt ttttcaggtc ggcaatttcg tggtactcga ctgcgaactt
      181 tttcggcgtc agcgcggcca aggtcaaaag cgataaactg gggagggagg cgatgatttt
      241 attgcgctca acaaagccgg ggagggtcaa accgagttga gtcaactcct cattgtgcgc
      301 ccggacgccg ctcatggcaa tcaagccgat tttcatctgc cgatcacctc ccaaagggtc
      361 aaaactccgg ccagggtcag actcaagacc ggaatgagaa agattgcccg cagtcttggt
      421 ttatgcgcca tgataaaaca acaagcaggc atcgccaccg ctccaacgac aaagccccac
      481 gaggcaacgt caatcgaacg gttgggcggg aggatctgct gggtggtgaa gtagaaaagt
      541 agattcagga ttgccagacc gaagaaagaa atgtggccca aacggtagag gcggcgcttt
      601 agactcgtgt acccgcccag ccactcttcc cggtgaaagt tcaagcccaa tagaaaaccg
      661 gtgacgaaac cgacgacaat ccagagccag gccaggatga agttgatttt gatcatggaa
      721 aaattcagag tcccccgttt tcagaccggg ctcatttccc ggacgcgtct ttggttgtgg
      781 gagcgctcca ggtcttcgcg aaacttcggg tgcgcgatct cgattaacgc ccgcaagcgt
      841 tcacgcaggt tcttgccgtg cagataggcc acaccgtatt cggtgacgat gtagtgcacg
      901 tcgccgcgcg tcgtcaccac acccgctcct tcgcgcaggg ccgggacgat gcgggagaaa
      961 ctctgccctt tggctgtcga cggtaaagcg atgaccgcct tgccgccttt cgaccgggcc
     1021 gcaccgcgca cgaaatcgag ttgtccgccg aagcccgagt aaatctgata cccgatggag
     1081 tcggaacaga cctggccggt caggtcgact tcgatggcgg agttgaccgc caccattttg
     1141 tcgttctggg cgatgaggaa aggatcgttg gtgtagtcgg cggggtggaa ctcaaaggcg
     1201 gggttgttgt gagcgaagtc gtagagccga cgtgaaccca agagaaaggt cgccaccacc
     1261 ttccccgggt ggagcgtctt tttcgccccg gtgatgacgc ctgattccat caactccatc
     1321 aggccgtcgc tcaccatttc ggtgtgcacg cccaggtctt tccggtcgcg caattgctgc
     1381 aggacggcgt ccggaattcc gccgatgccc gcctgcaggg tcgccccgtc ttccactaat
     1441 tcgccgacga agcgcccgat tttttcttcg atagcaccgg ggggttccgg caaaagctcg
     1501 ggcaggtctt cggcgacctc cacgattttg gcgagtttcg agaagtgcac gaacgaatcg
     1561 cccagggtac gcggcatttt ttcgttcacc tgcgcgacga cgaccctggc cgattcaagg
     1621 gcggcccgcg tggagaggca ctcgacgcct aaactgagaa agccgtgctc atccggcggc
     1681 gaagtttgaa tcagcgccac gtctatcggc aactgcgttc gcagaaggcg cgggatttcg
     1741 gagaggaaga ccgggatgta atcggcccgg ccggtctgga ccgccgcccg gtccgaaggg
     1801 ccaacgaaga gcgagttgtg ccggaaatgg ccctccatcc ccgggcggga gagcgggtcc
     1861 tcgcggcctt tgcccagaag cagaacgtgg atcacctcca cgtcatgcag atcgtccttg
     1921 cgtgccgcca gagcgtccaa gaggacgaac ggcgtggcgg cgttgccgct gatgtagatg
     1981 cgctcccggc tcttgacaaa cgcgaccgcc tcttcgggtg agccgatttt gcgccggtag
     2041 tcgtccagcc agctcatagg agccgctcca gcggtcggta ctcaaaagcg tactcgcggg
     2101 ccaggtgctc ctgcgtgacg aagccggcgt gggcgctgac gcctttggcc agatcggtca
     2161 tcccccgaag cgattcgcgc acgccccgtt cgacgagact tttcaagtac ggcagcgccg
     2221 cgttggtgag ggcgggggag gcggtgcgcg ccaccagact gctggcgttg ggcatgcaat
     2281 agtgccagac accctcttcc tggtagacgt attcttcgga gggggtgggc cgggaggttt
     2341 cgatgcagcc gccgttgtca atcgaaaaat caagaatcac cgcgccgcgc ggcatggtgc
     2401 ggaccatttc gcgtgtaacc agtatcggtg cacgcgcccc cggctccagg accgctccga
     2461 tcagaacgtc ggcgaagggg acgaattttt cgatattgac ccggttcgcc acggcggtca
     2521 ccagactgcc ggcgaaatat ttatccagtt cctccagccg acgcggatgt ttttccaata
     2581 agtataccat ggcgcctatt ccgcgcagag cccgggcggc gtggtagccg agtgtgccgc
     2641 cgcccaggat gaccacatcc gccgggggaa tgccgggtat cccgcccaag agcgtcccgc
     2701 gcccgcgctc cagcaggcgg cccgccagtt gcggcaccat tttgcccgcg atttcgcttg
     2761 tgggactgag caccggcagg cggccgtccg cttcctcaat cagttcgtag cccgtggcgg
     2821 tgattttttt gtccagaagc gtctggagcg tacttttggg tgcggtgatc aaatgcagaa
     2881 agccggtgag gatctgacct tcgtgcagga gcgggagttc gtcgtcagcc acgcggccga
     2941 ctttgcagat gatgtccgcg cgctggaatg cttcttcgtg cgacagaacg atctgggcgc
     3001 cggccttttc gtaggtggca tcggcgaaat gcgcaccggt cccggcttca cgctcgacca
     3061 ctacccgggc gccggccttg actaattccg ccacgccggc aggcgaaagc ccgacccggc
     3121 gctcttccat cccccgcccc ggcggtcgct cccgcgggac tccgacaatc ataggatgcc
     3181 tcgccggctc ccggcgtcaa cgtggttggc acacaattca agagtagaga agtcttttgg
     3241 catactcttt caatatacca agcgctctga gcgaccccaa atcaggggca aattgagagc
     3301 acaccagtag ggataagaac gcattcctct ttttgtaggc gaagccggaa gcggctcgtc
     3361 ccggggacga agtgaaaagt cttttcgcct tgacggcgag gtgagtgctc tattttggga
     3421 aagaagttca acccgctttt ggcggagcct ttgcccggca cttttcgcaa ggacagagcg
     3481 agcgcaggta gtcgaaggtg taaatgccgg tggaatgccc gtcgctccac tcaaaacgca
     3541 gggcgtagcg ccccaccggg tgaatgatgt tcgga
//
LOCUS       DBNGZA010000035         2445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_324581, whole genome shotgun sequence.
ACCESSION   DBNGZA010000035 DBNGZA010000000
VERSION     DBNGZA010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_324581"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16FU_00755"
     CDS             complement(<1..223)
                     /locus_tag="AC16FU_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992939.1"
                     /translation="MKKLLITPLVFLGLTSSGLTQPEQLGVFVEPSAANFFGFDARVM
                     AMGGAALANATSGSATNYNPAALARIHRIE"
     gene            complement(370..870)
                     /locus_tag="AC16FU_00760"
     CDS             complement(370..870)
                     /locus_tag="AC16FU_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992940.1"
                     /translation="MKRNWALFIAAAVGTGILGTGCYTILAHPEVDQEKRYSTRDYYE
                     NCSSCHQNYHGTYDYGYYPDFYYDNWRWNRYYLDPWWYNWYGYDYNAPPGQTLPAEPG
                     QKGERRRDLRGRSPGRQRIESGQSSESQRGESGKEEKVKEEKTKKDEKKKEEEKPEKG
                     ERRRVP"
     gene            complement(896..1717)
                     /locus_tag="AC16FU_00765"
     CDS             complement(896..1717)
                     /locus_tag="AC16FU_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992941.1"
                     /translation="MARRRMAVQWNCGIAGFLIVFGLAAEAKAQQKREPQPKRPLVYF
                     VAPRRIIDLPTAGTLPRGYFDMELRMFANEGVLTAVNIGLTQHFELGLSYGAEHALGN
                     QGVNWNPRAEFNVKLRPISETMLLPAIAVGFESQGFGGWDAEQGQKRYAVKSRGFYGV
                     VSKSYVFTGFATGFHGGVNYSFENDDGDRSPNLFGAMDMRFSDNVAFLGEYDLALNDN
                     NGDRYGKGRGYLNLGVRWVFSDQLDMEVDLKDLFQNRRGTRSFGREFRLLYIEHF"
     gene            complement(2013..>2445)
                     /locus_tag="AC16FU_00770"
     CDS             complement(2013..>2445)
                     /locus_tag="AC16FU_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992942.1"
                     /translation="FVGKVKSLYECGILDYYEMIDKNLFYAIVLEAINRGSYKIDYFR
                     SQPIPFLRVAIREEIDRFSMMISESQNEGSKQWSKPIELISIKKAEFSFIECFEWDRY
                     GYASYPYYLVRIQKLPKYPHFVGREALIETQDARVFYVDEK"
BASE COUNT          523 a          714 c          629 g          579 t
ORIGIN      
        1 gttcaattcg gtggatgcgg gctaaggcgg cgggattgta attcgtcgcc gagccggagg
       61 tggcgttggc caaggccgcg ccgcccatcg ccatcacccg ggcgtcaaac ccgaagaagt
      121 tggccgctga gggctcgacg aaaacgccca attgctccgg ctgggtcagg cctgacgagg
      181 tcaggcctaa aaagaccaat ggagtgatga gcagtttctt catagtgcac ctttttccaa
      241 aaatcatttt tgctccgggg cgaccgaggg cggtcgcccc acgcgaagta gtaccttccc
      301 cgcgtggacg gggcgccctc gccgcgctca gatacacttc tgaatcggct tccgccattg
      361 atacgtttct cacggcaccc ggcgccgctc tcccttttcc ggtttctctt cctctttttt
      421 cttctcgtcc ttcttggtct tttcttcctt caccttttct tccttccccg attctccccg
      481 ctgggattcg ctggattggc cagactcaat gcgctggcgt ccgggtgacc ggccccgcag
      541 gtcccggcgg cgctcccctt tctgccccgg ctcggcgggg agggtctggc cgggcggagc
      601 gttgtaatcg tacccgtacc agttgtacca ccagggatcg aggtagtagc ggttccagcg
      661 ccagttgtcg tagtagaaat cggggtagta gccgtagtcg tacgtgccgt ggtagttctg
      721 atggcaggaa ctgcaatttt cgtagtagtc gcgggtcgaa tagcgttttt cctgatccac
      781 ttccggatgc gccagaatgg tatagcaccc cgtaccgaga atgcctgtcc ccaccgccgc
      841 cgctatgaaa agcgcccagt ttctcttcat cgcgttcacc tcactttcac tacgtctaaa
      901 agtgctctat gtacaagagc cgaaactcgc ggccgaacga ccgggtcccg cgccggttct
      961 ggaacaggtc ttttaggtcg acttccatgt cgagttgatc ggagaagacc cagcggacgc
     1021 ccagattcag gtagccgcgc cccttgccgt agcggtcgcc gttgttgtcg ttgagcgcca
     1081 gatcgtattc gcccaaaaac gccacgttgt ccgaaaagcg catgtccatt gcgccgaaga
     1141 gattggggga gcggtcgccg tcgtcgtttt cgaaactgta gttgaccccg ccgtggaagc
     1201 cggtggcaaa gccggtgaag acgtaacttt tgctcaccac gccgtagaaa ccgcgcgact
     1261 tgacggcgta gcgcttctgc ccctgttcag catcccagcc gccgaacccc tgcgactcga
     1321 agcccaccgc aatggccggt aaaagcatcg tttccgaaat cggccgcaac ttgacgttga
     1381 actccgcccg cggattccag ttgacaccct ggttgcccag cgcgtgttcg gcgccgtagg
     1441 aaagccccag ttcaaagtgc tgggttaaac cgatattgac cgccgtgagc actccctcgt
     1501 tggcgaacat ccgcagttcc atatcgaagt atccccgggg cagggtcccg gccgtcggga
     1561 ggtcgatgat ccgcctcggc gccacgaagt agactaacgg ccgcttgggc tgcggctcgc
     1621 gcttctgctg cgcttttgcc tcggctgcca acccgaacac aatcaagaat cctgcaatcc
     1681 cacaattcca ctgcaccgcc atccggcgtc gggccatttt ccctccttgt gccatctgta
     1741 atttagacca ccagagggga ggcgtcaatg aaaactgcga acctgacgtg aagaatctgc
     1801 accatccagc gacgtggaaa gcaaatggga tgccttgaac ctttaagcga ccttgcaagg
     1861 agaagtcgtt tctaatggca atgaaaatgc tttaaaacag ttcatggaac tactgcgatg
     1921 cacatttttc tttcggggaa aagggctgag cgcgtcgggc gaccgcctcg gttgccccta
     1981 caaatttcgc cgctcgggag agcggcccta catcactttt catctacata aaatactcga
     2041 gcatcctgag tttcaataag agcttccctt ccaacaaaat ggggatattt gggcagcttc
     2101 tgaatcctca cgagatagta cgggtagctt gcgtagccat atcgatccca ttcgaaacac
     2161 tcaatgaatg aaaattcagc tttctttata gaaattagtt caatgggctt actccattgt
     2221 ttggatcctt cattctgaga ctcgctgatc atcatactga atctgtcaat ctcttccctt
     2281 atggcaactc ttagaaaagg aatgggttga gacctgaaat aatcaatttt ataggaaccc
     2341 ctattgattg cctcaagcac aattgcataa aagagattct tgtcaatcat ctcataataa
     2401 tcgagaatac cacactcata taaggatttc acttttccta caaaa
//
LOCUS       DBNGZA010000036         1585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1067007, whole genome shotgun sequence.
ACCESSION   DBNGZA010000036 DBNGZA010000000
VERSION     DBNGZA010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1067007"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="AC16FU_00775"
     CDS             complement(<1..422)
                     /locus_tag="AC16FU_00775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2992943.1"
                     /translation="MRHVPFGQLFINKLVIVALGLLLGVALAQAGQDRVLKLFGDLQN
                     VIISVSDRIKPSVVHIEVVQKFDNNKFETLGSGLIVDERGYILTNEHVVDNAQSVTVT
                     LPSKREYPAEVVGVDKQTDLALLKIDAPEKLTAAPLGNS"
     gene            706..>1585
                     /locus_tag="AC16FU_00780"
     CDS             706..>1585
                     /locus_tag="AC16FU_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR2992944.1"
                     /translation="MIKKFFTRENLPWVVFLILVSVAVGIILASGLDLPSRTLAGYQA
                     QAAGQEKFSGHSPFVAVAARVKDAVVNISAEKVVGRRPSFFDQFFFYPHPGRAKYLSL
                     GSGFIFDQRGYILTNNHVISGADNIQVKLSDESVYEAKIVGADPATDIAVLKIKASRD
                     LPSVVLGNSDSIFVGDWAIAVGNPFPQQGLDRTVTVGVISAKGRSNLVFGDQQPVYQD
                     YIQTDAAINHGNSGGPLVNISGEVIGINSAIATSSPDGGNVGVGFAIPINLAKAVLPS
                     LLKAGKVERGWLGVQPR"
BASE COUNT          345 a          427 c          435 g          378 t
ORIGIN      
        1 gagttgccca gcggtgcggc ggtcaatttt tccggcgcgt cgattttcag cagggccagg
       61 tcggtctgct tgtccacccc caccacctcg gccgggtact cgcgtttgga gggcaaagtg
      121 accgtcactg actgggcgtt gtccaccacg tgctcattgg tgagaatgta gccgcgctcg
      181 tcgacgatta agccggaacc cagagtctca aacttgttgt tgtcgaattt ctggacgact
      241 tcgatatgaa ccaccgaagg cttgatgcga tccgagacgg aaattatgac attttgcaga
      301 tcaccgaaga gtttcaagac ccgatcctgg ccggcctgcg ccagagccac ccccaggagt
      361 aatcctaaag cgacgataac taatttattg ataaatagtt gtccaaaggg gacgtgtctc
      421 atctgccttc tcagcttcgt catatccttc ttctcggcaa attatacgaa actttttatc
      481 aatcaaggag aaaaagcccg caacaaaaaa ggtgcaacgg cgtataatta tattgaagct
      541 gttttgttgt aagtaagttg ttgtacagtc gtaacttatc ggaagctggt ctgaagactg
      601 accaaaagaa cgaaaaccac attttaggtc tggcgcaaaa attgtccttg acaaaagggg
      661 ataaagggtt aagtttattt ttcttttggg ggagaaaaaa cgagcgtgat taagaaattt
      721 ttcacaaggg aaaatcttcc ctgggttgtc tttttgattc tggtctcggt ggccgtgggg
      781 atcatcctcg cctccggtct cgatctccct tcgcgcactc tggccgggta ccaggcccag
      841 gccgccggtc aggaaaagtt ctccggccat tccccatttg tggcggtggc cgcccgggtc
      901 aaagatgcgg tggtgaacat ttccgccgaa aaagtggtgg gccgcaggcc gagtttcttc
      961 gaccagttct ttttctaccc gcaccccggc cgggcgaaat atctgagtct cggttcgggc
     1021 ttcatcttcg accagcgtgg gtacatcctg accaataacc acgtcatttc gggcgcggat
     1081 aacatccagg tgaaattgtc cgacgagtcg gtctacgagg ccaaaatcgt gggcgccgac
     1141 ccggccaccg acatcgccgt tttgaaaatc aaagccagcc gggatctccc ctcggtggtt
     1201 ttgggtaatt cggactcgat tttcgtcggc gactgggcca ttgccgtcgg caatcctttc
     1261 ccccagcagg ggctcgaccg gaccgtcact gtcggggtga tttccgcaaa gggccgctcg
     1321 aacttggtct tcggcgacca gcagccggtc taccaggatt acatccagac cgatgccgcc
     1381 atcaatcacg gcaattcggg cgggccgctg gtcaatatct caggcgaggt aatcggcatt
     1441 aattccgcca ttgccacttc gtctccggac ggcggcaacg tgggtgtcgg ctttgcgatt
     1501 ccgattaacc tggccaaagc ggtgctcccc tcgttgctga aagcgggcaa agtcgagcgg
     1561 ggctggctgg gcgttcagcc gcgcg
//
LOCUS       DBNGZA010000037         6268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1915923, whole genome shotgun sequence.
ACCESSION   DBNGZA010000037 DBNGZA010000000
VERSION     DBNGZA010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1915923"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..538
                     /locus_tag="AC16FU_00785"
     CDS             143..538
                     /locus_tag="AC16FU_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR2992945.1"
                     /translation="MRKKNFLTYPKSAVTKARKLRRGMTDAEKKLWTFLRRNYLGTHF
                     RRQVPFGPYILDFLAINARLAVELDGSQHYEPKGKTRDTKRDVFLKEQGLAVLRFTDR
                     EVLGNINGVMQRIIEHIQQDRFSLPLRGG"
     gene            579..1742
                     /locus_tag="AC16FU_00790"
     CDS             579..1742
                     /locus_tag="AC16FU_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR2992946.1"
                     /translation="MIDVQTTRLRLEETEAHLLAPYATRNRDSRGRVYPEEEHPYRTR
                     FQRDRDRIIHSTAFRRLEYKTQVFVNHEGDHYRTRLTHTIEVSQIARSLARALRLNED
                     LAEAIALVHDVGHTPFGHAGEEVLNELLKDSGGFSHNRQTLRVVDLLEERYPEFPGLN
                     LTYEVREAIVKHGTSYGSPYPQEFSAKEQATLESQLVNIADEIAYNCHDVDDGLYSGI
                     LSVEELRQVELFRRLEEQARADFPQATPGRLQNVIVRRLINYEVSDVVTATLENLKAK
                     GVDSLEKVRDAGETLVGFSDSLAALNRELKEFLFRRMYRHFRLVRMADKARRIMTALF
                     AVYRDDPDQLPPTTRARLSREPVHQVIADYIAGMTDRFALLEYKKLFDPLERV"
     gene            1988..2398
                     /locus_tag="AC16FU_00795"
     CDS             1988..2398
                     /locus_tag="AC16FU_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR2992947.1"
                     /translation="MTFWRVKLPMQCYFYGVTRREKKSAQPGGVASFSIPDLGLSFSA
                     QFDGNREECQYAGLLALLEFVELNSNLFRKKTLEIYGDSFIVVHQVNLKMVCTRNLEP
                     YRNLALVYKKKISYTLNWIPPSQNRAQPGLALQA"
     gene            2648..3673
                     /gene="holA"
                     /locus_tag="AC16FU_00800"
     CDS             2648..3673
                     /gene="holA"
                     /locus_tag="AC16FU_00800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR2992948.1"
                     /translation="MPPRERKVKAYADLVREIAARKLGRFFLFYGEEEFLKKEALREF
                     EAALFKPEEKALNLQKLSGQQTNAEEVINAVSTAPMFGTHRLVEVHDAQRLSPPAKER
                     LIALLPKIPETGVTIFLADKTDFRQLFWKFFLDQGAAYEFASLLEKQALEWLFQRAKR
                     SGIEVEEEAAWALVEKLGTDAGQIASELEKLATLAQKSQKITKSLVEQAVGFSQRFSV
                     YQLLDAVGERNLPQALVIYNDLVSYRESLTQIVYWLGEHFYRLYVVTQSDNGKLADPR
                     VAALHPFVLNKCRRQAKNFSVGQLSSGLSQIAQADADIRLSKVKSELVVETLLVRLCR
                     LSGDKRE"
     gene            3698..4297
                     /locus_tag="AC16FU_00805"
     CDS             3698..4297
                     /locus_tag="AC16FU_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2992949.1"
                     /translation="MKSEGEAVRDVATPALPEEAALVRRARGQDKKAFAELVRIYQKR
                     IWALVYRLTKERDAAWDLSQDTFVKAYFAIGSFREGMPFYPWLATIAVHLALNYLKRE
                     KRQVKFTDEEMQEHVLESQPATSNPIEEMISAEMQAKLEGEIDRLPPEYRAVFVLRVY
                     EEMSYDQIAKTLEISQGTVMSRLFRARQRLLAALKEHLA"
     gene            4314..5441
                     /locus_tag="AC16FU_00810"
     CDS             4314..5441
                     /locus_tag="AC16FU_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2992950.1"
                     /translation="MTTNRCGFDLAKLSAYVDGALSPKDHLTVEAHLQTCPDCREFIA
                     GLQKAEASLKGAPAPELTPEEEARFAPAVMRQIERLEQKPAPVSAGAKKFFLFRSVGL
                     RWAAAVAAVVLVAITSTLLLKEKSELLQPQRRLGQRTPEGRLAPPLTTPETTQTGLIG
                     NESLAKNEETIPAQEAAKKPVAESSRVAESLEATPQAELAVPAPAPEESLGEGARVEE
                     PAKVEDKDLAAQKKVPEKGEPLFVNPQIAPRQLAAETPETTTTRARDVLHMRGGRDAE
                     YLFSGTTAFQKSAQKAAIDGLRAQYLVELDSIRRVALLASLCALEFAEAQKSRDTTEL
                     KLAREDITILLSTLASDSIQTKKWKGKLERINKLQPASQKK"
     gene            5831..>6268
                     /locus_tag="AC16FU_00815"
     CDS             5831..>6268
                     /locus_tag="AC16FU_00815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR2992951.1"
                     /translation="MDESEIDVRRRLEMVETQIAKRGIADPLILAAMRQVPRHFFVPE
                     KFHALAYADEPQPIGYDQTISQPYIVGLMTELLGLKGGERVLEIGTGSGYQAAILAEI
                     AGVVFTMEIVRQLAERARERLRELGYVNIWCKIGDGSQGWPEHA"
BASE COUNT         1437 a         1812 c         1745 g         1274 t
ORIGIN      
        1 cgacccctcc ctgccctccc ctcaagggga gggaaagaaa tacgttcttg atagccgtgg
       61 agtattctcc ctctccttga ggagagggtt ggggagaggt cggaagtgtt ggttaggcgc
      121 gatacgattt tgaggtttaa aggtgaggaa gaagaacttt cttacatacc caaagtcagc
      181 ggttactaaa gcgcggaaac tccggcgcgg gatgaccgat gcagaaaaga agctctggac
      241 ttttttgaga cgtaattatc tcggtactca ttttcggcgg caggttcctt tcggccctta
      301 catccttgat tttcttgcca tcaatgcaag actagctgta gaattagatg gtagccaaca
      361 ctatgaacca aaaggtaaga ctcgtgatac aaaacgggat gtatttctga aagaacaggg
      421 acttgctgtc ctgcggttta cagatagaga agtcctgggt aacattaatg gggtgatgca
      481 aaggattatt gagcatatcc agcaagatag gttttccctc cccttgaggg gagggtgagg
      541 gaggggtcag tattgaaacg ggagaaagag acgttaacat gatagatgtc caaaccaccc
      601 gcctgcggct cgaagaaacc gaagcccatc tgttggcccc ctacgccacc cgcaaccgcg
      661 acagccgagg ccgcgtctac ccggaagagg agcaccccta ccgcacccgc ttccagcgcg
      721 accgcgaccg catcatccac tccaccgcct tccgccgctt ggaatacaaa acccaggtct
      781 tcgttaacca cgaaggcgac cactaccgca cccgcctcac gcacacgatt gaagtctcgc
      841 aaatcgcccg ctcgctcgcc cgcgccctcc gcttaaatga agacttagcc gaagccatcg
      901 cgctggtgca cgacgtaggc cacaccccct tcggccacgc cggggaagag gtgctgaacg
      961 aactgctcaa agactccggc ggcttttccc acaaccggca gacgttgcgc gtcgtcgatc
     1021 tcttggaaga acgctacccc gagttccccg gcctgaacct cacctacgaa gtgcgcgagg
     1081 caatcgtcaa acacggcacc agttacggct cgccgtaccc gcaggaattt tccgccaaag
     1141 agcaggccac gctggaaagc cagttggtca acatcgccga cgaaatcgcc tacaactgcc
     1201 atgacgttga cgacgggctc tactccggca tcctctcggt agaagaattg cgccaggtgg
     1261 aactcttccg ccgtctcgaa gaacaggcgc gggcggactt tccgcaggcc actcccgggc
     1321 gcctgcagaa tgtcatcgtc cgtcggctga tcaactatga ggtgtcggac gtggtcaccg
     1381 ccacactgga aaatctcaag gcgaagggcg tcgattcgct ggaaaaagtg cgggacgctg
     1441 gggagacact ggtggggttc tccgattcac tggcggcgtt aaaccgggaa ctgaaggaat
     1501 tccttttccg gcggatgtac cgccacttcc gcctggtacg gatggccgac aaggcgcggc
     1561 gcataatgac cgccctcttt gccgtctacc gggacgatcc cgaccaatta cccccgacca
     1621 cccgggcgcg gctctcccgg gagccggtgc accaggtaat cgccgactac atcgccggta
     1681 tgaccgaccg cttcgccctc ttggaataca agaaactgtt tgatccgctg gagcgagtct
     1741 gacctcaagc gtataaccct ctctgcacct ctcctgtagg actatgcata gcgattttgc
     1801 ggtgttccgc agcggtgttt tgggctcgta atcgagccaa cccacacaaa ggagttctac
     1861 aaaaggccat ttcacttatc ttacagttct acatacactt acgctccggc ggctttcccc
     1921 ttctctccgg aaatccgcat ccagagcggg cttgcgctaa tcttccatcg gcacagggtt
     1981 tgcacttttg acgttttgga gagtcaagtt gccgatgcag tgttacttct acggggtcac
     2041 gcggagagag aaaaagagcg cgcaaccggg cggcgtcgcc tccttctcca tccccgatct
     2101 ggggctctcc ttcagcgccc agttcgacgg caaccgggag gagtgccagt atgcggggct
     2161 tctggcgctg ttggagttcg tcgaactgaa ctcgaatctt tttcgcaaaa agacgctgga
     2221 aatctacggc gacagtttca tcgtggtaca ccaggtgaac ctcaaaatgg tctgcacccg
     2281 gaacctggag ccctaccgca acctcgcctt ggtctacaag aagaagatca gctacactct
     2341 gaactggata ccccccagtc agaaccgcgc ccagccgggg ctggcgcttc aggcgtaagt
     2401 cctattcata acttctaagc aacccctccc ttgatccctc ccctcaaggg gagggagttt
     2461 tcattatttc ataattcctc tcctggagga gagggtaggg tgtcgaagcg actcttcgcg
     2521 aagactcgct ccgagaggtc attcaggcaa agacgccggt caggtgaccg gcgctactcc
     2581 ccttgagccc tcttttgctt tgaacgcgtc caccggcagg ttatatattt caacagccag
     2641 ggagtcgatg ccaccgagag agagaaaggt caaagcgtac gccgatctgg tgcgggagat
     2701 agctgcccgc aaattgggcc gcttctttct gttctacggc gaagaagaat ttctgaaaaa
     2761 agaggctctc cgggaattcg aggcggctct cttcaaaccg gaagagaagg ccctcaatct
     2821 ccagaaactt tcgggccagc agaccaacgc cgaagaggtt atcaacgccg tctccaccgc
     2881 tccgatgttc gggacgcacc gcctggtcga agttcacgac gcccaaaggc tgtcgcctcc
     2941 ggcgaaggag cgtttgatcg ccttgctccc gaagattccg gaaaccggtg tcaccatatt
     3001 tctggcggac aaaaccgatt tccggcagct tttctggaaa tttttcctgg accagggtgc
     3061 ggcctacgag tttgcatccc tgctggaaaa gcaggcgctc gagtggcttt tccagcgcgc
     3121 caagaggtcg ggcattgaag tggaagagga ggccgcctgg gcgttggtcg aaaaattggg
     3181 caccgatgcc gggcagatcg cttccgaact ggaaaagttg gccacgttag cacaaaaatc
     3241 ccaaaaaatt acaaaatctc tggtggaaca ggcggtggga ttttcacagc gattctcggt
     3301 ctaccagctc ttagatgccg tgggcgagcg caacctgccc caggcgctcg tcatctacaa
     3361 cgatttggtc tcctaccgtg aatcattaac ccaaatcgtc tactggctgg gggagcattt
     3421 ctaccggctc tacgtggtca cccaaagcga caacggtaaa ctggccgatc cccgggtggc
     3481 ggccctccat cccttcgttt tgaacaaatg ccgccggcag gccaaaaact tttccgtcgg
     3541 tcaactctct tccggcctca gccagattgc ccaggccgat gctgatattc gactctccaa
     3601 ggtcaaatcg gaactggtgg tggagaccct tttggtccgg ctctgccgtc tttcgggaga
     3661 caaacgggaa taaaacgccg ccccggctgt atttcctatg aaaagcgaag gtgaagcggt
     3721 tcgagatgtc gccaccccgg cactgcccga agaagccgcg ctggtgcggc gcgctcgcgg
     3781 acaagataaa aaagcgtttg cggagctggt ccgaatctat caaaaacgca tctgggcttt
     3841 ggtctatcgc ctgaccaaag aacgcgatgc cgcctgggat ctgtcgcagg atacttttgt
     3901 gaaggcctac ttcgccatcg gctcgttccg ggaagggatg cctttctatc cctggctggc
     3961 caccattgcg gtgcatctgg ccttgaatta tctcaaacgg gagaagcgcc aagtcaagtt
     4021 taccgatgag gaaatgcagg agcatgtttt ggaaagtcaa ccggcgactt caaatccgat
     4081 agaggaaatg atcagcgcgg aaatgcaggc caaactggag ggtgaaattg accgcctgcc
     4141 gccggagtat cgcgccgtat tcgtgttgcg ggtttacgag gagatgagtt acgatcagat
     4201 cgccaagacg ctggagattt cccagggtac ggtgatgtcg cgcttgttcc gcgcccggca
     4261 gcgccttttg gccgcgctca aagaacattt agcttaaggc aaaacaggag accatgacca
     4321 ctaatcgctg tggatttgat ttggcaaaac tttccgccta cgtggacggt gctctgtctc
     4381 ccaaggacca tttgacagtc gaagcgcacc tgcagacctg cccggactgc cgggagttta
     4441 tcgccggtct ccaaaaggcg gaagcctcac tcaagggggc acccgctccg gaactgaccc
     4501 cggaggagga agcccgcttc gctccggcgg taatgcgcca aattgagcgg ctggaacaaa
     4561 agccggcccc ggtctcggcc ggggcaaaaa agtttttcct cttccggtcg gtgggtttgc
     4621 gctgggccgc cgcagtcgcg gccgtcgttt tggtggcgat caccagcacc ctattactca
     4681 aggagaaatc tgaactcctg caaccgcagc ggcggttggg ccagaggaca ccggaagggc
     4741 gcctggctcc gcctctgacc acgccggaga cgacccaaac gggtttgatc gggaatgaat
     4801 cgctcgccaa gaacgaggaa acaatccctg ctcaagaagc ggcgaagaaa ccggttgcag
     4861 aatcatctcg agttgcagaa tcactggagg caactcccca agccgagctc gcagttcccg
     4921 cacccgcccc ggaggagtcg ttaggggaag gtgcgcgggt ggaggaaccg gcaaaggtcg
     4981 aagacaaaga cctcgcggct caaaagaagg tcccagagaa aggagaaccg cttttcgtca
     5041 atccccagat tgccccccgg caactcgcgg ctgaaacacc ggaaactact accacccggg
     5101 ctcgcgatgt cttgcacatg cggggtggcc gcgatgcgga gtaccttttc tccggtacaa
     5161 ctgcatttca gaaaagcgcg caaaaggcgg ccattgacgg tctgcgggca cagtacctgg
     5221 tggaactcga ttccatccgg agagttgctc tgctcgcttc cctctgtgca ctggaatttg
     5281 cggaagccca aaagagccgc gataccaccg aacttaagct ggcccgggag gatattacca
     5341 ttttactcag caccctggcg tctgacagta ttcagacaaa gaaatggaaa ggaaaactcg
     5401 aacgaatcaa taaacttcag ccagcatcac aaaagaagta gttccttctc ctgacctcac
     5461 cccgaccctc tcctacagga gagggagtaa gttctttcct cttttggtga tggacaagtc
     5521 aatagataaa gggtgctctt tgcacttgaa tgacccttcc tttccctccg tcaccgtaga
     5581 gggaagaagg agtaagttcc ctcccctgtg aggggagggc agggaggggt caggcacgcc
     5641 cacaaggggc tgtgctacca agaaactcag aattgggaac ttggttccag aggattatcc
     5701 ctccccctgt aggggaggag aaggagggat cgttttgttt tcttgcggtt tcccggcgac
     5761 tcctcagttg acgcccgttc gtcattgccc tatcatcact ccgcagcgcc ttgcaagcgg
     5821 ggagctccct atggacgaat cggaaataga cgtccgccgc cgtctggaga tggtggaaac
     5881 ccaaatcgcc aagcgaggga ttgccgaccc cctaatttta gcggcgatgc gccaggtacc
     5941 ccgccatttc ttcgtcccgg aaaaattcca tgccttagcg tatgccgacg aacctcaacc
     6001 catcggctac gaccagacga tctcccagcc gtacatcgtc ggcttgatga ccgaactttt
     6061 gggcttgaag ggcggcgagc gggtgctgga aatcggcacc ggctccggct accaggcggc
     6121 gattttggcc gaaattgccg gggtcgtttt caccatggaa atcgtccgcc agctggcgga
     6181 gcgcgcccgg gaacgattgc gggaattggg ctacgtcaac atctggtgca aaatcggcga
     6241 cggctcacag ggatggcccg agcacgcg
//
LOCUS       DBNGZA010000038         6215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1172724, whole genome shotgun sequence.
ACCESSION   DBNGZA010000038 DBNGZA010000000
VERSION     DBNGZA010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1172724"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="AC16FU_00820"
     CDS             <1..2227
                     /locus_tag="AC16FU_00820"
                     /inference="COORDINATES: protein motif:HMM:NF052712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992952.1"
                     /translation="LRFFRLREPEHFIATQLDGKTGPDEITQRLTEKFGIDLPAAAVA
                     EFIGRLEKAGFTEGSLSEHQIARARYQRPDQGKPSLLKRLVFIKLKAFDPDNFLTRLA
                     PYTNIFYTKGFVILSGALFLVAANITLTNKEAFLAVRLGDLFRVGGILFAVFTIFLVI
                     ALHELAHAVTCKRYGGRVNDMGVLLLYFQPCLYTNLSDAWLFPQKGKRLLVTFVGAYF
                     QLITWAVATLLWGVSDPDSLAGRFFFIASAVTFISILFNFNPLIKLDGYYLLSDWLEI
                     PNLRQKAFRYLGNRLKALILGLETKPETGDLRERRIYLAYGIFALAYSAFLVGWILSK
                     VAQLLISGLAGFGFLLFLALVGAMFTKPMVEVAREIEAAIAQKGPQFYRSRRFLVKAG
                     VAALLLAVVFFVRYDLRVSRDCRIEALETFTVTNREASFLETNLFREGEQEERATDLV
                     RMFGSDFSAANFVPKVREGTRVRAGDTLAVILSNLYQNELLKAQANYREAKARLELLL
                     RGAKPAEIQKQKQNVEQLRLERVQKERDFDKSKQLFEKRLISQEAYNQAQSDLDIAQA
                     AFAQAQKGLEVLTTPPRAEEVQAARADMDQYQGRINTILGQLEQGAVKSPISGTVLAV
                     GTKGELLSIANLESVRVLVPAQENDLDIIDTGQSIRAKVPGYPSADFAGTVFKISRQG
                     EDYRGKVSFQVTGIVPNPAHLLKPGMTGKAKIYCGRVPIYQLFWRKFWRFVKVEFWSW
                     W"
     gene            complement(2341..3336)
                     /locus_tag="AC16FU_00825"
     CDS             complement(2341..3336)
                     /locus_tag="AC16FU_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2992953.1"
                     /translation="MKRLGLLAAAGVLLFGGLFPLALGDDRTELLQSRDGQSNHEPTS
                     GDALDPSKRDTVRVATVDALGANVVVPVYLFNSKKRLAGSLVSLKYGSDLVTCDSVSF
                     ADTRLVASEFRVAQVRLQEQVIQFTLLSVSGHNGDGDDEGDDGNEDEDNPLTHSVPPK
                     DFGVLAFLHFRATGAPSPSNHFAPIDTFLSVPLVVRFRGEKIDPVKHKQNAELRRATF
                     VPGGINFLTSPVTRERDGALPKSFELFQNHPNPFNPSTTLRFAVPQTSRVELAVYNIL
                     GQKIRTLLDEVVPAGVYSTEWNGTDDAGMGAPSGIYFARFAAGSRVATQKMTLLK"
     gene            complement(3721..4233)
                     /locus_tag="AC16FU_00830"
     CDS             complement(3721..4233)
                     /locus_tag="AC16FU_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992954.1"
                     /translation="MKVFALAVLATFLGAAPLKAGEPGAMKNTPGFEKLKSLVGTWQA
                     KTKDGQVTTVSYKIISAGSAVMETMSHSKDEAAMVTIYHLDGDNLMMTHYCSANNQPR
                     MRAEPVTGDPKALTFSYLDATNLASPDDGHMHKLVVTFQDKDHFTQEWTWWEKSKEAG
                     THVFQFERKK"
     gene            complement(4366..4950)
                     /locus_tag="AC16FU_00835"
     CDS             complement(4366..4950)
                     /locus_tag="AC16FU_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR2992955.1"
                     /translation="MKKRKTPNLKRELVATEKELVKLKARLIKLRHRLPKEEVKNYTF
                     RGPEGGTAALSSFFGDKDDLILIHNMGKGCAYCTMWADGFNGVLHHLENRAGFVVVSP
                     NDPETQKQFAASRGWKFRIYSSQGTTFNKDMGFENDKGGAQPGVSVFRKDKDGKVFRV
                     SSAGFGPGDDYCNVWHLFDLLSGGPAGWEPKFTY"
     gene            complement(5044..5511)
                     /locus_tag="AC16FU_00840"
     CDS             complement(5044..5511)
                     /locus_tag="AC16FU_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2992956.1"
                     /translation="MLKETAPSGRETTLQLRRIFSAPREKVFKAWTDPSEMKKWIIPE
                     EGFTVPAVEVDLRVGGEFRIEMLSPKGSRHTAVGRYREIAALEKLVFTWSWLEYPVHG
                     ETLVTIEFRDLGGATERSFTHELFPTKASRDDHQRGWNGCLDQLTKYCAALGQ"
     gene            complement(5505..5864)
                     /locus_tag="AC16FU_00845"
     CDS             complement(5505..5864)
                     /locus_tag="AC16FU_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR2992957.1"
                     /translation="MAYDEKLAGRVRGLLVRRKGVAEKKMFGGLCFLLKGKMFCGVLK
                     EDLVARVGPEKYQAALARPHVRPMDFTGRPIKGFVFVGPGGCLTEQSLSNWLTPALEY
                     ASTLPPKSKRLKGGKTC"
     gene            complement(5949..>6215)
                     /locus_tag="AC16FU_00850"
     CDS             complement(5949..>6215)
                     /locus_tag="AC16FU_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HIR2992958.1"
                     /translation="HGALDYKECVGDDLNPKWAVPFPKIAKLKPGETVVFAYIVFKSR
                     AHRDRVNAKVMKDPFMNDPEYKDKPMPFDVKRMAYGGFKVLVDA"
BASE COUNT         1295 a         1838 c         1679 g         1403 t
ORIGIN      
        1 cctccgcttc ttccggctgc gcgaaccgga acacttcatc gccacccagc tggacgggaa
       61 aaccgggccg gatgaaataa cccaaaggct cactgaaaaa tttgggattg acctgcctgc
      121 cgcggcggtg gccgagttca tcggccgatt ggaaaaagcc gggttcaccg aagggagctt
      181 gagcgaacac cagattgccc gggccagata ccagcgtccg gatcaaggca aaccgtcgct
      241 tctgaaacgg ttggttttca tcaagctcaa ggccttcgat ccggacaatt tcttaacccg
      301 gctcgccccc tacaccaaca ttttttacac caagggcttc gtcattcttt cgggggcgct
      361 cttcctggtt gccgcgaaca tcaccctgac caataaggaa gcgttcctgg cggtgcgttt
      421 gggcgatctc ttccgggtgg gcgggatact ttttgccgta tttacgattt ttctggtgat
      481 tgccctgcac gaactggccc acgcggtcac ctgcaaacgc tacggcggcc gggtcaacga
      541 catgggggtg ctcctgttgt acttccagcc ctgcctttac accaacttga gcgacgcctg
      601 gctcttcccc cagaaaggga agcgcctgct cgtgaccttt gtgggggctt acttccagct
      661 catcacctgg gcggttgcca ccctcctctg gggggtgagc gaccccgatt ccttggccgg
      721 ccgctttttt ttcatcgcct cggcggtcac tttcatatcg attctcttca atttcaaccc
      781 cctgatcaaa cttgacggct actacctcct gagcgactgg ctggagattc ccaacctgcg
      841 ccagaaagcg ttccgttacc tcgggaaccg gctcaaagcc ctgatcttgg gcctggaaac
      901 aaaaccggaa acaggcgacc tccgcgaaag aagaatttac ctggcctacg gcattttcgc
      961 cttggcctat tcggcttttc tggtcggctg gattttgtcc aaagtggcgc aactgctgat
     1021 cagcggccta gctggcttcg gcttcctgtt gtttctggct ctggtggggg ctatgtttac
     1081 caaaccgatg gtggaagttg cccgcgaaat tgaggcggcc atagcgcaga aaggaccgca
     1141 gttttaccgt tcccggcgtt tcctggtcaa ggccggtgtt gccgcactcc tcttagccgt
     1201 cgttttcttt gttcgctacg acctgcgggt ttcgcgcgac tgccgtatcg aagcgctgga
     1261 gaccttcacc gtcaccaacc gggaggcttc ttttttggaa accaacctct tccgggaggg
     1321 ggaacaggaa gagcgcgcca ccgaccttgt gcgcatgttc gggagcgatt tttcggcggc
     1381 caatttcgtc cccaaggtac gcgaaggaac ccgggtgcgg gccggagaca cattggcggt
     1441 gatcctctcc aacctctacc agaatgaact gctcaaagcc caggccaact accgcgaagc
     1501 caaggcccgc ctcgaacttt tgctccgggg cgccaagccg gccgaaattc aaaaacagaa
     1561 gcagaatgtg gaacagctgc gtctggagcg tgttcagaaa gagcgcgatt ttgataagtc
     1621 aaaacagctc ttcgagaaaa gattgatctc gcaggaagcc tacaatcagg cccaaagcga
     1681 cttagacatc gcccaggccg ccttcgccca ggcccagaag ggtttggaag tgctcaccac
     1741 cccgccccga gccgaagagg tacaggcggc ccgggccgat atggatcagt accagggacg
     1801 gattaatacc atcctggggc aactggagca gggcgcggtg aaaagcccaa tttccgggac
     1861 cgttttggcc gtaggcacca aaggggaatt gctttccata gccaacctgg aatcggtgcg
     1921 ggtgctcgtc ccagcgcagg agaatgattt ggacatcatc gacaccggcc agtcgatccg
     1981 ggccaaagtt ccggggtacc ccagtgccga cttcgccggg acggtgttca aaatctcccg
     2041 gcagggggag gattaccggg ggaaagtttc attccaggtg accggcatcg tgcccaatcc
     2101 ggcgcacttg ctcaagccgg ggatgaccgg caaggccaag atttactgcg gccgggtgcc
     2161 gatctaccaa ctcttctggc gcaaattctg gcgcttcgtg aaagtggaat tctggtcgtg
     2221 gtggtagcta aactctccgg gccggcacca agtttcaacc gctctgctgc caactgcttt
     2281 ttgtaaacaa aaaatccccc gcctgaggcg ggggattttt tgaccgcatg tgcctctgac
     2341 ttacttcaaa agcgtcatct tttgcgttgc cacccgactg ccggcggcga agcgggcaaa
     2401 gtaaatcccg ctgggggcac ccatgccggc atcatcggtg ccgttccatt cagtggagta
     2461 gacgccggcg ggaaccactt cgtccagaag tgtccggatc ttctggccta agatgttgta
     2521 aaccgccagt tcgacccggc tggtctgggg cacggcaaaa cgcagggtgg tgctgggatt
     2581 gaacggattg gggtggttct ggaataactc aaacgatttc ggtagggctc cgtccctttc
     2641 ccgcgtcacc ggcgaagtca ggaaattgat ccctccggga acgaaggtgg cccggcgcaa
     2701 ttccgcattc tgcttgtgct ttaccggatc tatcttctct ccgcggaagc gtaccaccaa
     2761 gggtaccgag agaaaggtgt cgatcggcgc aaagtggttg gaaggtgaag gcgcgccggt
     2821 cgcccggaaa tggaggaacg ccagtacgcc aaagtctttg ggtggtaccg aatgtgtgag
     2881 cggattgtct tcgtcctcat ttccgtcatc accttcatcg tcaccgtcgc cgttgtgacc
     2941 ggagacagac aacagggtga actgaattac ctgttcctgc agccgcacct gagcgacccg
     3001 aaactcactg gcaaccagcc gggtgtcggc gaaactgacg ctgtcgcagg tgaccaggtc
     3061 gctgccgtat ttgagcgaaa ccagagaccc cgccaagcgt tttttcgagt tgaacagata
     3121 aaccgggacg acgacattcg cgcccaaagc gtccaccgtg gccacgcgta cggtatcacg
     3181 cttcgatggg tcaagagcgt cgccgctggt cggttcatgg tttgactggc catccctgga
     3241 ttgcaaaagt tctgtgcgat catcgcccaa agccaaagga aacaacccgc cgaacaaaag
     3301 taccccggcc gccgccaata gtcccaatcg cttcatctcg ccttactcct ttctttcagt
     3361 ttcgatagac caactcaccc acctaagacg caaaaagcgt gcaaaaagtc ttaatatctt
     3421 actgatgccg aactttacgg tatatcgtgt tgagtatcaa gggattagga tgggccaaat
     3481 ggataacatc ggcgcgtctt gctgccgatg tcctatcccg gccaagccac cagttgcaaa
     3541 gagactcatc cgttcaggtt gagatagtgc gcccgccgtt tccgttccga tttttcggag
     3601 agggtgatgc agtagtgccg tttgaatttt ccgcttagca aaaatacccc ccgtgcgtcg
     3661 gcgacggaga ggtcttccga cccgggcgga acagaaccgg cccgggtcgg aaatgccgac
     3721 ttacttctta cgctcgaact gaaaaacgtg tgtacccgct tccttgcttt tttcccacca
     3781 ggtccactcc tgcgtgaagt ggtctttatc ctgaaacgtg accaccagct tgtgcatatg
     3841 tccgtcgtcc gggctcgcca ggttggtagc atccaagtag gagaaggtca gggccttcgg
     3901 atcgccggta accggttcgg cccgcatgcg cggctggttg ttggccgagc agtagtgcgt
     3961 catcatcagg ttgtcgccgt cgagatggta aatcgtgacc atggccgcct catccttact
     4021 gtggctcatc gtttccataa cagccgagcc ggcggaaatg attttgtagg agaccgtggt
     4081 gacctgtcca tctttggttt tggcctgcca cgtgccgacc agggacttaa gtttctcaaa
     4141 gccgggggtg ttcttcatgg cacccggctc gccggctttg agaggcgctg ctcccagaaa
     4201 cgtggcgagc acggctaacg caaatacttt catgacatac ctcctcgcgt gtctgtttgt
     4261 cgtttggcag ccgtcttgat tttcattacg gctgccggta acctccaggt ttcggattct
     4321 tctcaggtaa acctcctttc ctcttcgtgg gcctccagag gggggctaat aggtgaactt
     4381 cggctcccag ccagctggac cgccggacaa cagatcaaac aggtgccaga cgttgcaata
     4441 atcatccccg gggccgaacc cggcggacga cacccgaaat accttgccgt ccttgtcctt
     4501 gcggaaaacc gagacgccgg gctgcgcgcc gcccttgtcg ttttcaaatc ccatgtcttt
     4561 gttgaatgtg gttccttggc tggagtaaat ccggaatttc cacccccggc tggcggcgaa
     4621 ctgcttctgc gtttccggat cgttgggcga aacgaccaca aagccggccc ggttttcgag
     4681 gtggtgcaaa acgccgttaa acccatccgc ccacatcgtg cagtaggcac accctttgcc
     4741 catgttgtgt atcaggatca ggtcgtcttt gtccccgaaa aaactggaaa gcgcggccgt
     4801 tcctccttcc gggcctcgaa aagtataatt tttcacctcc tcctttggca accggtgccg
     4861 gagcttgatc aatcttgctt ttagcttcac cagttccttt tcagttgcca caagctctcg
     4921 cttcaaattc ggggtttttc gcttcttcat atttcctccg atcatgagaa cctctcccga
     4981 aaccgaacag aacgacagat ttcaatcggg caagctgaac ctcggtgcag ccaaatttct
     5041 caactattgc ccgagtgccg cacagtattt tgtcaattgg tccaggcagc cgttccagcc
     5101 ccgttggtga tcgtcccgcg acgctttggt cggaaagagt tcatgagtga acgacctttc
     5161 agtagcacct cccagatcgc gaaattcaat cgttaccaga gtttctccgt ggacgggata
     5221 ttccagccag gaccaggtga aaaccagctt ttccagagcc gcaatctccc ggtaacggcc
     5281 aacggccgta tgccggcttc ctttggggga cagcatttca atgcgaaatt ccccgcccac
     5341 ccgcaaatcc acttcaaccg ccggcacggt aaatccttct tccggaataa tccatttctt
     5401 catctcggag gggtcggtcc aggccttgaa aaccttctcg cgcggggcgg agaatatccg
     5461 ccggagttgg agggttgttt ctctacccga aggggccgtc tccttcaaca tgttttccct
     5521 ccctttaaac gcttggattt tgggggtagg gtggaggcat attcgagcgc cggggttagc
     5581 cagttggaga gagactgttc cgtcaggcaa ccgccgggcc cgacgaaaac gaaacccttg
     5641 atggggcggc cggtgaaatc catcggccgg acgtgcggcc gtgccaacgc cgcctgatat
     5701 ttttccggac cgacccgcgc taccaaatcc tccttcaaaa cgccgcagaa cattttaccc
     5761 ttgagcagaa agcaaagacc tccgaacatc ttcttttccg ccactccctt acggcgaacc
     5821 agaagccccc gcacgcgtcc cgccagcttt tcatcgtagg ccatcgatat tcactcccgt
     5881 gtcacccttc catacaccag tcctataccc ggacagccaa cagaaactga atgccgggga
     5941 aaaaagcatt aggcatcgac gaggaccttg aatccgccgt aggccatccg cttgacgtcg
     6001 aaaggcatcg gcttgtcctt atactccggg tcgttcatga aggggtcttt catcactttt
     6061 gcgttgaccc ggtcacggtg cgctcgcgac ttgaacacaa tgtacgcgaa caccaccgtc
     6121 tctcccggct tgagcttggc gattttcggg aacggaaccg cccactttgg attgaggtca
     6181 tcgccaacgc attccttgta gtccagggcg ccgtg
//
LOCUS       DBNGZA010000039         3892 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1067501, whole genome shotgun sequence.
ACCESSION   DBNGZA010000039 DBNGZA010000000
VERSION     DBNGZA010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1067501"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16FU_00855"
     CDS             <1..208
                     /locus_tag="AC16FU_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992959.1"
                     /translation="RKFGKARIRQTILFAPSKDGEPDFKKMAELTRQCVSFPLIESFR
                     QAYGKAKEKSKHNRDSINHQHPKN"
     gene            364..1191
                     /locus_tag="AC16FU_00860"
     CDS             364..1191
                     /locus_tag="AC16FU_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992960.1"
                     /translation="MKHIGSVLSVIGVVIGLIGITLAVHYHSQSIQERVPTFYVEPVR
                     AKIFDATGQAPSELQVLHRGRAISERSVTAVQIYFWNEGELPIRKADILEPLAIELWP
                     PAEILAAHLVKQSRKVINFSLGAVPESTSNRLPLSFDILEKSDGAAIQIIFAGEPDAP
                     VALKGTIVGVGAPKLLSLSKGGGERPNPLVTFKLTQLMRNSLLAIGVVALLLIGALWL
                     AVRRRGQKSVMKFGIFVIVTAIFFGIFWGMYMAYDAEKTSSTGVPSGIWLTGESFKP"
     gene            1211..1714
                     /locus_tag="AC16FU_00865"
     CDS             1211..1714
                     /locus_tag="AC16FU_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2992961.1"
                     /translation="MDLDRIRALYDYNRWANAKILGAAAPLAPEAFTKDLGSSYRSVR
                     DTLVHILGAEWIWLERWKGTSPRALLPFEDFPTVDSIRKRWQEIERGQEQFLSGATEE
                     KLLASLSYINIKGLPFTYPLWQALQHVVNHSTYHRGQVITMLRQLSAQPVATDFLAFY
                     DERFGKR"
     gene            1732..3015
                     /gene="clpX"
                     /locus_tag="AC16FU_00870"
     CDS             1732..3015
                     /gene="clpX"
                     /locus_tag="AC16FU_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR2992962.1"
                     /translation="MNQQRPKNSNHTSDSTREGKTCSFCGRPAEEAGVLLSSPQAAIC
                     EECLLRGRELLSTQKTKKVAEEKPPDLPKPAEIKAFLDQYIIGQERAKKTVSVAVYNH
                     YQRVFHPEATAEVELEKANILLLGPTGTGKTLMAQTLAKMLHVPFSIADATVLTEAGY
                     VGEDVENILVRLLQSADYNLTRAQRGIIYIDEIDKIARKEANPSITRDVSGEGVQQGL
                     LKILEGTISNVPPKGGRKHPEQPFIQVDTKNILFVCGGAFDGLEKIIARRVGQKVVGF
                     AASPLQRGQGKIGEILAQVEPEDLLQYGLIPELIGRLPVVCAMDELDEAALYDILVKP
                     KNALVKQYRKLFELEGIDLEFTEEALREAVAQAQKRKTGARALRSILEEAMLDIMYEL
                     PSKPEVARCAITREVIAKTGAPEYLYRKEAAKKSA"
     gene            3070..3513
                     /locus_tag="AC16FU_00875"
     CDS             3070..3513
                     /locus_tag="AC16FU_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2992963.1"
                     /translation="MAGTLRRSADWAPVFLRLALAIVFIMAGSGKLFGWFGAPGFKGV
                     SGYIGMLGFKPAAFWTALLAVTEFGGGLAMLFGILTRWAGLGLAIVMAVALVRVHIKW
                     GLAVNGGDGHFDLPLVLLLVALAMTVTGAGKWSLEAKMKKAPAMT"
     gene            3582..>3892
                     /gene="yihA"
                     /locus_tag="AC16FU_00880"
     CDS             3582..>3892
                     /gene="yihA"
                     /locus_tag="AC16FU_00880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR2992964.1"
                     /translation="MLQITKAEFAKTVFEPGEAPRDQRPQIAFAGRSNVGKSSLLNIL
                     CRRKNLAQVSKTPGKTKALNFFLVNEQIWFTDLPGYGYAKVEKKLREQWGTLVEKYLQ
                     ET"
BASE COUNT          874 a         1008 c         1200 g          810 t
ORIGIN      
        1 acgcaagttc ggtaaggcga gaataaggca aacgattctt tttgctcctt caaaagatgg
       61 tgaacctgat tttaagaaaa tggcagagct tactcgccaa tgcgtttcct ttcctctcat
      121 agaatcattc agacaagcgt atgggaaggc gaaagaaaaa agtaagcaca atcgagactc
      181 tataaaccac cagcacccga agaattagag ccgtattcgt tagattaaca tcgaacggaa
      241 acgaagaaga gcatggattt agatcgtata ccggcattgt acgactacag ccgctgggct
      301 aatgccaatc cgcctcaggc ggactgggcg cgggtgtaac ccgctggagg taacgacgtt
      361 ttgatgaagc atatcgggtc ggttctatcg gttatcgggg tcgtcatcgg ccttatcgga
      421 attaccctgg ccgtgcatta ccatagtcaa tccatccagg agcgcgtgcc cacgttttac
      481 gtcgagccgg tccgggccaa gatcttcgat gccacggggc aggcgccctc cgaacttcag
      541 gttttgcacc ggggccgcgc aatcagcgaa agaagcgtaa cggcggttca gatttatttt
      601 tggaacgaag gggagctgcc catcagaaag gccgatattc tcgagccgtt ggcgattgaa
      661 ctctggccgc cggcggaaat tttggcggcc cacctcgtca aacagagccg aaaggtgatc
      721 aacttttcgc tgggcgcggt gccggaaagt accagcaacc gtctgccgct ctccttcgat
      781 atactggaga aatcggacgg agccgcaatc cagatcatct ttgcgggtga accggacgcc
      841 ccggttgcct taaaagggac cattgtcggc gtgggtgcgc ccaagctgct cagtctgtcc
      901 aagggaggcg gcgagcgccc caatcctctg gttacgttta agttgacaca actgatgcga
      961 aattcgctcc tggccatcgg cgtagtcgcc ttgctcttga ttggcgcgct ctggctggcc
     1021 gttcggcgga gagggcaaaa gtcggtcatg aaattcggaa tttttgtgat tgttaccgcc
     1081 attttctttg gaattttttg gggaatgtat atggcctacg atgcggaaaa gacgtcgtcc
     1141 acgggagtgc cctccgggat atggctaacc ggcgagtcat tcaagccgta atcataaggc
     1201 agaaaaaaac atggatttag accgcatacg ggcattgtac gattataatc ggtgggccaa
     1261 tgccaagatt ttaggcgcgg cggcgccgct tgcgccggag gcgttcacca aagacttggg
     1321 gagcagttac cgctcggtgc gggatacgct cgtccacatt ttaggggcgg agtggatctg
     1381 gttagagcgc tggaagggta cttcgccccg ggcgcttttg ccattcgaag atttcccgac
     1441 cgtcgatagc atacgaaaac ggtggcagga gattgagcgc ggacaagagc agtttctctc
     1501 cggcgcgacg gaggagaagc ttttggcatc cctttcctac attaacatca aggggctacc
     1561 gtttacgtat cccctctggc aggcgctgca acacgtggtg aaccactcca cctaccaccg
     1621 ggggcaggtg attacgatgc tccggcagct gagcgcccag ccggtggcga ctgatttttt
     1681 ggcgttctac gacgaacggt tcggcaagcg ataaccagag atggagaagg catgaatcaa
     1741 cagcgtccca agaacagtaa ccacacatcc gattccactc gtgaagggaa aacctgctcc
     1801 ttttgcgggc ggccggcgga agaagcgggg gttctgctct ccagcccgca ggcggctatt
     1861 tgcgaagaat gtctcttgcg cgggcgggaa ctgctcagca ctcagaagac caagaaagtt
     1921 gcggaggaaa agccgcccga cctgcccaag ccggcggaga tcaaggcctt tttagaccag
     1981 tacatcatcg gccaggagcg ggcgaaaaag acggtctcgg tggcggtcta caaccactac
     2041 caacgggtgt ttcatccgga ggcaacagcc gaagtcgaat tagaaaaggc caacattctt
     2101 ctcctcggcc ccaccggcac gggcaagact ttgatggctc agacgctcgc caagatgctc
     2161 cacgtcccct tttccattgc cgatgccacg gttttgaccg aggcggggta cgtgggcgag
     2221 gacgtcgaaa acatcttagt acgtctcttg cagtcggcgg attacaacct gacccgcgcc
     2281 cagcggggca ttatctacat cgacgaaatc gacaagatcg cccgcaagga agcgaatccc
     2341 tccataaccc gggacgtttc gggggagggg gtacagcagg ggctgctcaa aatcttggaa
     2401 ggcacgattt ccaacgtgcc gcccaaagga gggcgcaagc acccggagca gccgttcatt
     2461 caggtcgata ccaaaaacat tcttttcgtc tgcggcgggg cgttcgacgg gctggaaaag
     2521 atcatagcgc gtcgtgtggg acagaaggtc gtggggtttg ccgccagtcc gttgcagcgg
     2581 ggacagggga aaatcggcga gattctggcc caggtcgaac cggaagacct gctgcagtac
     2641 ggattgattc ccgaattgat cggccggctg ccggtggtct gtgccatgga tgagttggat
     2701 gaagcggccc tgtatgatat tttagtcaag ccgaagaacg cgctcgtcaa gcagtaccgg
     2761 aagctgttcg agctggaagg gatcgacctc gaattcacgg aagaggcgct gcgggaggcg
     2821 gtggcccagg cgcagaagcg gaaaaccggt gcccgcgccc tgcgctcgat cttagaagag
     2881 gcgatgctcg acatcatgta cgagttgccg tccaaaccgg aggtcgcgcg atgtgcgatc
     2941 acccgggagg tgattgccaa gacgggagcg ccggagtacc tttaccggaa ggaagcggcg
     3001 aaaaagagcg cgtagtcgag cgaaaaagat gctaggcgga ggcgttcacc atcaaccaga
     3061 ggagggaaga tggcgggtac attgagacgg agtgctgact gggcgccggt cttcttgcgg
     3121 ctggctttgg cgattgtgtt catcatggct ggctcgggga aacttttcgg ctggttcggc
     3181 gcccccggtt tcaaaggtgt ttccgggtat atcggcatgc tggggttcaa gccggcggcc
     3241 ttctggacgg cgcttctggc ggtcaccgaa ttcggcggcg ggctggcgat gctctttggc
     3301 atcctcaccc gttgggccgg cctgggactg gcgattgtta tggcggtggc cttggtgcgg
     3361 gtgcacatca agtggggtct ggcggtcaac ggcggggacg gccactttga cctcccgctc
     3421 gtccttttgc ttgtggccct ggcgatgacg gttaccggtg ccgggaagtg gtctttggaa
     3481 gccaagatga aaaaagcccc ggcaatgacc tgagctcgct tgaggaagtc atttgactta
     3541 agccgggcgg gtgaaaaacc cgcccgttgt gtgtcggtta tatgcttcaa atcaccaagg
     3601 ccgaatttgc caagaccgtt tttgaaccgg gcgaagcgcc ccgggatcag cgccctcaga
     3661 ttgctttcgc cgggcggtcg aatgtaggga agtcgtcact tttgaatatc ctgtgccggc
     3721 ggaaaaattt ggcccaggtt tccaaaactc cgggcaagac gaaggcgctc aatttctttc
     3781 tggtcaatga gcagatctgg ttcaccgatc ttcccggcta cggctacgcc aaagtggaaa
     3841 agaagcttcg ggagcagtgg gggactttgg ttgagaagta tctccaggaa ac
//
LOCUS       DBNGZA010000040         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2127831, whole genome shotgun sequence.
ACCESSION   DBNGZA010000040 DBNGZA010000000
VERSION     DBNGZA010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2127831"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="AC16FU_00885"
     CDS             <1..763
                     /locus_tag="AC16FU_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2992965.1"
                     /translation="DSLKLATRLRGKERLRKATVVDPTRYEAFVNLGVAYLKLNQPES
                     ALVCYETAHKLNPKDVAIISSLAGNYFTQKRFSEAVKLYQEIVAIDPQNKDALLAMGA
                     AYDQLGETEKVKETYLRVLQIDPKDKNVLFNMGLFALRAVADTTKHFQAILEKNPNDT
                     KAQAEQQRIQKIYYASAESLFVRASQADTTDKYAWHYAGVCARILDHVDQALQYQLKA
                     TAVDNGFKDAYQELAVLYTLKNMPDKAKEAEAKSK"
     gene            876..>1552
                     /locus_tag="AC16FU_00890"
     CDS             876..>1552
                     /locus_tag="AC16FU_00890"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR2992966.1"
                     /translation="MTERLIEVAFPLSVRHQFTYRVPPELRERVVPGMRVLAPLGQRQ
                     KIGFVVGEATHRPKLIKEITDLLDPEPIVVPDLLRLTRWMADYYFSSWGECLQAVLPA
                     GIQGQVAETIRWNPEVKAESILAGNHWSAAEKNFLTLLSQEHSLKISVLKRTFGTREL
                     YARLIDFERRGFLVLETSIQRHQSEPLTHQYVRLTIGLAKEQQTTFAQYLAALPEKQK
                     KKRGTLQ"
BASE COUNT          361 a          479 c          403 g          309 t
ORIGIN      
        1 cgacagtctc aaactcgcta cccgcctgag gggcaaggag cgcttgcgga aagccaccgt
       61 tgtcgatccc acccgctatg aggccttcgt caacttaggc gtcgcatatc tgaagctcaa
      121 tcagcccgag tcggccttgg tttgttacga gacggcccac aaactgaacc ccaaggacgt
      181 cgccatcatc tccagtctgg cggggaacta cttcacccag aagcgcttct cggaggcggt
      241 gaaactgtat caggagattg ttgccatcga tccccagaac aaagatgcgc ttttggccat
      301 gggcgcggcc tacgaccagt tgggcgaaac cgaaaaagtg aaggagacct acctgcgggt
      361 gctccagatc gacccgaaag acaagaacgt gctgttcaac atggggctct ttgccctccg
      421 cgccgtagcc gacaccacca agcacttcca ggcaatcctg gagaaaaatc ccaacgatac
      481 gaaagcccaa gccgaacagc agcggatcca gaaaatctac tacgcctcgg ccgaatcgct
      541 ctttgtccgg gcctcccagg ccgatacgac ggacaagtac gcctggcact acgccggcgt
      601 ctgcgcccgt attctggatc acgtcgatca agcgctgcag tatcaactca aggccaccgc
      661 cgtcgataac ggcttcaaag acgcctacca ggaactggcg gtcctctaca cgctgaagaa
      721 tatgccggac aaagccaaag aggccgaagc gaaatcgaag taacctcagt agccgctggc
      781 ggtgtttcga gggtgaagaa tttatttcgc ctgtagccac gcttttctga gcgtggcttt
      841 ctttttgggt cttttgctct acatattact gactcatgac cgaacgctta atcgaagtcg
      901 cttttcccct ctcggtgcgg caccagttca cctaccgcgt cccgccggaa ctgagggagc
      961 gggtcgttcc cggcatgcgc gttctggctc ccctcggcca gcggcaaaaa atcggttttg
     1021 tcgttgggga agccacccac cggccgaaac tgatcaaaga aatcactgac ctgctcgatc
     1081 cggagccgat cgtcgtcccc gaccttttgc gcctcacccg ctggatggcc gactactact
     1141 tctcttcctg gggcgaatgt ttgcaagcgg tcctgccagc ggggattcag ggccaggtgg
     1201 ccgaaaccat ccgctggaat ccagaagtca aagcggagtc gatcctggcc ggcaaccact
     1261 ggtcggcagc agagaaaaac ttccttaccc ttctctccca agagcattcg ctcaaaatat
     1321 ccgtactcaa gagaactttc ggcacccggg aactctacgc ccgtttaatc gatttcgagc
     1381 ggcgcggctt tctggtgctg gaaacctcca tccagcgcca ccagagcgag cctttgaccc
     1441 accagtacgt gcgtctgact attggtctcg ccaaggaaca gcagacgacc tttgcccaat
     1501 atctggcagc acttccggag aaacagaaaa aaaagcgggg cactttgcag tt
//
LOCUS       DBNGZA010000041         2743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2127893, whole genome shotgun sequence.
ACCESSION   DBNGZA010000041 DBNGZA010000000
VERSION     DBNGZA010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2127893"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="sucD"
                     /locus_tag="AC16FU_00895"
     CDS             <1..805
                     /gene="sucD"
                     /locus_tag="AC16FU_00895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR2992967.1"
                     /translation="TSVIYVPAAFAADAIYEAADAGLNLVVCITEGIPISDMLRVYNY
                     LKTTGTRLVGPNCPGLITPGATKVGIIPGHITLPGPVGIVSRSGTLTYEIIYNLTLAG
                     VGQSTCLGIGGDPIIGTSFVDVLALFQADPQTKAVVLIGEIGGTDEEEAAKFIRQEMT
                     KPVVGFIAGITAPPGKRMGHAGAIIAGGAGTAKEKIEAFRAVGVRVAESPDQVVPLVK
                     ERLHMTAEKKPGTVRARRKPARTAIPKPPARTRKQSRMVVAAAHRVRKA"
     gene            819..1229
                     /gene="ndk"
                     /locus_tag="AC16FU_00900"
     CDS             819..1229
                     /gene="ndk"
                     /locus_tag="AC16FU_00900"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR2992968.1"
                     /translation="MAERTLFIIKPDAVGRNLIGKIIARIEEARFRVRNLKMVRLTEA
                     EARRFYYVHEGKPFLPELVQFMSSGNVVPMVLEKENAVSGLRTLIGATDPKEAAPGTI
                     RADFATDKGKNAVHASDSLESAKFEIDFFFGNER"
     gene            1276..2202
                     /gene="mdh"
                     /locus_tag="AC16FU_00905"
     CDS             1276..2202
                     /gene="mdh"
                     /locus_tag="AC16FU_00905"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR2992969.1"
                     /translation="MKKKVAVIGAGNVGASCALYIAEENLADVVLVDIIEGVPQGKGL
                     DLTQAGPIRGYDCTITGTNDYAQIAGSQLVVVTAGLPRKPGMSREDLITMNAEIVGKV
                     MDGVVKYAPEAFVLVVSNPLDIMTYHAWKKSGFPHQRVFGQAGILDSIRFRSFVAAEL
                     GVSMSDTQALVLGGHGDTMVPLPRYTTVAGIPITELLPKEKVDALSKRTRDGGTEIVA
                     LLKTGSAYYAPAAASVAMVDAVLSDRKRFVAASAYVTGQYGLSDLYIGVPVLLGAKGV
                     EKVIELKLQPDELAALQKSASIYKDMLKVLKY"
     gene            complement(2492..>2743)
                     /gene="glmU"
                     /locus_tag="AC16FU_00910"
     CDS             complement(2492..>2743)
                     /gene="glmU"
                     /locus_tag="AC16FU_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR2992970.1"
                     /translation="RQKKIREVNTGTFCFQADPLWRALARIQNENRQAEYYLTDVIEI
                     FARRNRRVLAVTAPDPWETMGINSRAQMAEVERLFFGGE"
BASE COUNT          595 a          779 c          843 g          526 t
ORIGIN      
        1 cacttcggtc atttacgttc ctgccgcttt tgccgctgac gcgatttatg aagccgccga
       61 cgccgggctc aacttagtag tctgtatcac cgagggaatt ccgatcagcg acatgctgcg
      121 cgtctacaat tatctgaaaa caaccggaac gcgcttggtc gggccgaact gtcccggctt
      181 aatcaccccc ggcgccacca aagtgggcat cattcccggg cacatcaccc ttcccggccc
      241 cgtggggatc gtctcgcgct ccggcacgct gacgtatgaa atcatctaca atctcacgct
      301 ggcgggggta ggacagtcca cctgcctcgg catcggcggc gatccgatca tcgggacgag
      361 ttttgtggat gttttagccc tcttccaggc cgatccgcag accaaagcgg tggttttgat
      421 cggcgaaatc ggcgggaccg atgaggaaga agcggccaag tttatccggc aggagatgac
      481 caaaccggtg gtaggattca tcgcgggtat caccgcgccg ccgggaaagc ggatggggca
      541 tgcgggagcg atcattgccg gtggagcggg aacggccaag gaaaagatag aagcgttccg
      601 agcggtcggc gtacgggtgg ccgaaagtcc ggaccaggtg gtgccgctgg tcaaagagcg
      661 cctgcacatg accgccgaaa agaaacccgg tacggttcgc gccagacgca aaccggcacg
      721 gaccgccatt cccaaacctc ccgcccgtac ccggaagcag tcccgcatgg tcgtggcggc
      781 ggcgcaccgg gtgcgtaaag cgtaaaagga caaggacagt ggccgaacga acattattca
      841 tcatcaagcc cgacgcggtc gggcgcaacc tcattggaaa aatcatcgcc cgcatcgaag
      901 aggcgcggtt ccgcgtcaga aacctgaaaa tggttcgctt gactgaagcg gaagcccgga
      961 ggttctacta cgtccacgaa ggcaagccgt tcctgccgga attagtgcag ttcatgtctt
     1021 ccggaaatgt ggtgccgatg gttttagaga aagaaaacgc cgtcagcggc ctgcgcaccc
     1081 tgatcggcgc caccgatccc aaagaagccg cgcccgggac gatccgggct gatttcgcca
     1141 cagacaaagg gaaaaacgcg gtgcacgcct ccgattcgct ggagtcggcg aaatttgaaa
     1201 tcgatttctt ctttggcaac gagaggtaat ttttttgtgt tcgatcgtga aacctttcac
     1261 aaaccggagg gaaagatgaa aaagaaagtg gcagtcatcg gggcggggaa tgtcggcgcc
     1321 tcctgcgcat tgtacatcgc ggaagaaaat ttagccgatg tcgtcttagt ggacataatc
     1381 gagggagtgc cccaggggaa ggggctggac ttgacccagg ccgggccgat ccggggctac
     1441 gactgcacca tcaccggcac caacgactac gcgcagattg ccggctcgca actggtggtg
     1501 gtgacggccg gcctgccgcg caagccgggg atgagccggg aagacctgat caccatgaac
     1561 gcggagatcg tcgggaaagt gatggacggg gtcgtcaaat acgccccgga ggccttcgtg
     1621 ctggtggtct ccaatccgct cgacatcatg acctaccacg cctggaaaaa atcggggttt
     1681 ccgcaccagc gcgtcttcgg gcaggccggg attttagatt cgatccgttt ccgatcattc
     1741 gtggcggcgg aattgggcgt ttcgatgagc gacacgcagg ccctcgtttt gggcgggcac
     1801 ggcgatacga tggtgccctt gccgcgctac accaccgtgg ccgggattcc catcaccgaa
     1861 cttttgccga aagaaaaagt tgatgcgctc tcgaagcgaa cccgcgacgg cggcactgaa
     1921 atcgtcgcct tacttaaaac cggcagcgcc tactacgcac cggcggcggc ctccgtcgcc
     1981 atggtggacg ccgtcctttc cgaccgcaag cgtttcgtcg cggcttcagc ctacgtcacc
     2041 ggccagtacg ggctttccga tttgtacatc ggtgtgccgg tgcttttagg cgccaaaggc
     2101 gtggagaagg ttattgaact gaagttacag ccggatgaac tggcggcatt gcagaaatcg
     2161 gcatcaattt acaaagatat gctgaaggtg ctgaagtatt gagtggttga attccgggaa
     2221 aagagaacca aaggcgaccc cgcctttggc ggggccgagg ttagaaacca cggctacgaa
     2281 cgtcgacagg cgggtgccga ggctgggaag cctcggctat ggaggaaaat ctcgtttccc
     2341 atgggtcagg cttccagcct gaccaaaatc ttcgaatttg gggtcacctc ccttattgtc
     2401 cttcccgtgt ggcccaagaa aaacgacgct ctttcagcgc cgccgaggtt ggtcggtacg
     2461 actctgacga gaaacctcgg ctacgactga attattcgcc cccaaaaaag aggcgttcga
     2521 cttcggccat ctgggcccgg gagttgatgc ccatcgtttc ccaggggtcg ggtgcggtca
     2581 ccgccaaaac gcgccggttc cgccgggcaa agatttcgat gacgtcggtg aggtagtact
     2641 ccgcctgccg gttttcgttc tggatccggg cgagcgcccg ccagagcgga tcggcctgaa
     2701 agcagaaggt gccggtgttg acttcgcgga tcttcttctg ccg
//
LOCUS       DBNGZA010000042         6383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1916939, whole genome shotgun sequence.
ACCESSION   DBNGZA010000042 DBNGZA010000000
VERSION     DBNGZA010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1916939"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="lpxD"
                     /locus_tag="AC16FU_00915"
     CDS             complement(<1..882)
                     /gene="lpxD"
                     /locus_tag="AC16FU_00915"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR2992971.1"
                     /translation="MPVTLGEIAQKTGGTLLGDPAVAITGVAGIETAGPGQLTFVANQ
                     KYRSLLVGTQATAVIISPDVATDSIKTALIQHPNPYYAFLVALRLFHPQERLYPPGIH
                     STAVVGKNVTLAEGVHLGPCVVLAEGVSVGEHSALLSGTYVGRNSKIGNDCLIYPNVT
                     AREGVTIGDRVIVHSGTVVGSDGFGFAKEGGVYHKIPQVGGVVVEDDVEIGANTTIDR
                     ATMGNTIIGRGTKIDNLVQIAHNVAIGENSIIVAQVGISGSTRLGKNVTLAGQVGLVG
                     HLTIGDNVIVAAKSGIHR"
     gene            complement(886..1413)
                     /locus_tag="AC16FU_00920"
     CDS             complement(886..1413)
                     /locus_tag="AC16FU_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2992972.1"
                     /translation="MSARQMLAVAAALVGLTAGVAFGQEGKIGFIDSNRLRNELKEFK
                     DAQAKLDKEAVDWKKHADSLQQAITTSETELERQRIFLSDEKRKEKESEIGLKKQELV
                     RYNTEIYDPVAGKYYRRNVELTKPILDRINQALERLATENNYVLIFDAVNGSIAYGRK
                     GLDLTDKLLQELAGR"
     gene            complement(1568..3805)
                     /gene="bamA"
                     /locus_tag="AC16FU_00925"
     CDS             complement(1568..3805)
                     /gene="bamA"
                     /locus_tag="AC16FU_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR2992973.1"
                     /translation="MCLWALPAFSQTQEYQIVSVEVQGNRVADRSLIQNVAGLAIGSR
                     LDAGGVADAIRRIYGLNLFSDVAIEGDLQGNNGIALVIQVQEYPKIRRIDIAGNKKVK
                     TEEIRTKLTLREGQTASPVAVQENVNRIRSLCEEKGYYLAAVESKSATEGSADEVVLS
                     FRINEGEKVKLEFIEFAGASAYPPDKLRGKIHGGGFKAERYEQDKEKLIEFYQKNGYT
                     DAAILGDTTWVDSTRRFMHALITLGEGKRFHFGQVAFSGDSLFGQQRLASQVKFKPGE
                     IFNQKKYDQTLGNLYALYQEEGYLYARIEDRKEPQDSLVNLTYEITQGIPAKINRVII
                     EGNQKTKDKVIRRELSVRPGQTFRRSLLLRSVRDVMQLNYFANVTPDYAVQPTGDIDL
                     VIKVEEKPTGQIQVGAGYSGQDKLVGTIGLGIPNLFGNGQRLDLNLDFGNRRNSVSLG
                     FTEPWFRDTPTSVGFDLFHLNRRFFEDFTEGRTGFSLRVGRRLRWPDNYFRLFGNYRL
                     ESLNYHDFSDDYRSRTQSNPSSLVNTDWPKVTSSAGLTLSRDSRDLPQFATSGAVFSW
                     SSEYAGGPLGGYFQYHKHIFEASKFQKLVWKFVLAGKARVGWVDNPKGEQKVPFIEKF
                     SPGGVDPDGVIRGYVDRSIGPYDSQGFVQGGRSVLTYNLELQFPIVPQQIYGLFLADA
                     GNAWASGREVTPFAHLFKSAGFGIRLTVPGIGLIGFDFAYGFDQSRFVQGRRWRPHFQ
                     VGTTF"
     gene            complement(4032..4355)
                     /gene="rplU"
                     /locus_tag="AC16FU_00930"
     CDS             complement(4032..4355)
                     /gene="rplU"
                     /locus_tag="AC16FU_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR2992974.1"
                     /translation="MYAIVEIGGMQFPVREGEVVEVPRLGAKAEDQVTFDKVLLVAGE
                     KGTAVGNPYLPKASVLGEVVREARGEKIIVFKFKRRTKYRRKRGHRQWYTAVKINKIQ
                     APQIA"
     gene            4503..6047
                     /locus_tag="AC16FU_00935"
     CDS             4503..6047
                     /locus_tag="AC16FU_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992975.1"
                     /translation="MVGIRFGEKNVLLVVGLAALAVAAWTAFASSGTWEDDAYIFFQY
                     AKNLAAGQGMAFNSGEVSFGVTSVLWTLILGFLSWVFHLGPVVMAKLASIVLFSASVT
                     ILTRLVIDCTQNSLLGFWTGLLVAVYPHLVLHAVSGMEIPLNLFLLTVLTVSFFRYGE
                     KHFWLYGILIGLAFLTRPENALWLPLFVWLAATSRGQFGLRIAALLGTCVGVVLPWEL
                     YLYSHTSLFLPPTRDGKLLLFLPVQYGVTLDQFRHLSLAARLDLAGRASVNVAKAIFL
                     NKGGVLFLPFLLVAGYFVARGKIALGRFGLALAGYGFGLVALFVLFFPLVKQRYFVHL
                     YPFLIFISVLTGCRLWQELRGRFKILIRPLWAKVGLVTVLLIAPLAGFFAARKFASMS
                     REQSIRAEVGRWLKTNTPPDARVALEPIGAVAYFADRYVVDLGGLINPDIWPFMSRGP
                     DSPPDALFDILVRKRVTHVVDYSQHPWAGKVVDLYPERFLLAAQISSPYPPAGYNQYD
                     IYKLNK"
BASE COUNT         1207 a         1953 c         1753 g         1470 t
ORIGIN      
        1 tctatggatg cccgacttgg ccgccacgat cacgttgtcc ccaatggtca ggtgccccac
       61 caggcccacc tgcccggcca aagtaacgtt tttgcccaga cgggtgctcc cggaaatccc
      121 cacctgcgcc acgatgatgc tgttttcgcc tatagccacg ttatgagcga tctggactaa
      181 gttgtcgatc ttcgtccccc gcccgatgat ggtattcccc atcgtcgccc ggtcgatggt
      241 ggtattggca ccgatttcca cgtcgtcctc gacgacgact ccgcccacct gcggaatttt
      301 gtggtagacg ccgccttcct tggcaaagcc gaacccgtca ctgccaacga ccgtacccga
      361 gtggacaatt acccggtcgc cgatggtgac cccctcccgc gccgtgacat tggggtaaat
      421 caaacagtca ttcccgattt tggaattgcg tcccacgtag gttccggaga ggagcgctga
      481 atgctcgccg actgacacac cttccgccaa cacgacacaa ggaccgaggt gaacgccctc
      541 cgccagggtg acgttcttcc cgaccacggc agtagaatga attcccgggg gatagagccg
      601 ctcctgcgga tggaacaacc gcagggccac gagaaaggca tagtagggat tcggatgctg
      661 gatcaacgcc gttttgatgg aatcggttgc gacgtcgggt gatataatca ccgctgtggc
      721 ctgcgtcccg acaaggagcg aacggtattt ctgattggcg acgaaggtca actgccccgg
      781 cccggcggtt tcaatccccg ccacaccggt aatggcgacg gccgggtccc ccaagagcgt
      841 cccgccggtt ttctgggcga tttctcctaa ggtcacgggc atgctctacc tgccggctaa
      901 ctcttgcagg agtttgtcgg tcaaatcgag gcccttgcgg ccgtaggcaa tgctgccatt
      961 caccgcatcg aaaatcaaaa cgtaattgtt ttccgtcgcc agccgctcca aggcctggtt
     1021 gatccggtct aaaatcggct tggtcagctc tacgttccgc cggtagtact tgcctgccac
     1081 cggatcgtag atttcggtgt tgtagcgaac cagttcctgt tttttcagac cgatctcgga
     1141 ttctttttcc ttccgcttct cgtcggagag gaaaatccgc tgccgttcca gttccgtctc
     1201 cgaggtcgtg atcgcctgct ggaggctgtc ggcgtgcttc ttccagtcga ccgcttcctt
     1261 gtccagcttg gcctgcgcgt ccttgaactc tttcaattcg ttgcgcagcc ggttggagtc
     1321 gatgaagccg atctttccct cctggccgaa ggccacaccg gcggtaagac cgaccagggc
     1381 ggctgcgact gcaagcattt gacgtgctga catacttttc ctccgtttca ttgaccaaaa
     1441 tacagaaaaa gtctttcaga agaaatggta atggtagggg tttgattaat caaaccccta
     1501 cctcagcgaa ccgaaaggga aacacgagaa ttacggaact gaagcaaggg acctcttact
     1561 gagagcacta aaacgtcgtc cccacctgga agtggggccg ccagcgccgc ccttgaacga
     1621 accggctctg gtcaaaaccg taggcgaagt caaacccgat caggccgatc cccggcaccg
     1681 tcagccggat gccgaacccc gccgacttga acaggtgcgc aaacggcgtt acctcccgcc
     1741 cggaggccca cgcattgccg gcgtcggcca gaaacaagcc gtaaatctgc tggggcacga
     1801 tcgggaactg gagctcgagg ttgtaggtca aaacgctccg gcctccctgc acaaagccct
     1861 gcgagtcgta cgggccgatg cttcgatcga cgtacccgcg aatcacccca tcgggatcga
     1921 cgccgcccgg cgaaaatttt tcgatgaagg ggactttctg ctcccctttg ggattgtcca
     1981 cccaccccac ccgcgccttc cccgctaaga cgaacttcca gaccagtttc tggaatttgc
     2041 tcgcttcgaa gatgtgcttg tgatactgga aatacccccc cagaggcccg ccggcgtact
     2101 ccgaactcca ggagaagacg gcgccggagg tggcgaattg cggcaggtcg cgggaatcgc
     2161 gggagagcgt cagtccggcg ctggaggtaa ccttcggcca gtcggtgttg acgagcgagc
     2221 tgggattgct ctgcgtccgg ctccggtaat catccgagaa gtcgtggtag ttcaaacttt
     2281 ccagccggta gttgccgaac agccggaagt agttgtcggg ccagcgcagc cgccgcccca
     2341 cccgcaggga gaaaccggtg cggccctcgg taaagtcttc aaagaagcgc cggtttaagt
     2401 ggaacaggtc gaatccgacg gatgtcggag tgtcgcgaaa ccagggctcg gtgaacccta
     2461 aagagaccga gttccgccgg ttgccgaaat ccagattcaa atcgagccgt tgcccgttgc
     2521 cgaagaggtt cggaatcccc agaccgatgg tgccgactaa tttgtcctgc ccgctgtagc
     2581 cggcgcccac ctgaatctgg ccggtcggtt tctcttcgac tttgatcacc agatcgatgt
     2641 cgcctgtcgg ctgcaccgcg tagtcggggg tgacgttggc gaagtagttc aactgcatca
     2701 cgtcccgtac cgagcgcagc aaaagggagc ggcggaaggt ctgccccggc cggaccgaaa
     2761 gctcgcggcg aataacctta tccttggtct tttgattccc ctcgatgatc acccggttga
     2821 ttttggccgg aatcccctgg gtgatttcgt aggtcaaatt gacgagcgaa tcctgcggct
     2881 cttttctgtc ctcgatccgg gcgtagaggt acccctcttc ctggtagagg gcgtagaggt
     2941 tgcccaaggt ctggtcgtac tttttctggt tgaagatttc gccgggctta aacttcacct
     3001 ggctggccaa acgctgctga ccgaacaaac tgtccccgga gaaggccacc tggccgaagt
     3061 ggaagcgttt cccttcccca agggtgatta acgcatgcat gaagcggcgg gtcgaatcta
     3121 cccaggtcgt gtcccccagg atcgccgcat cggtgtagcc gttcttctgg tagaattcga
     3181 ttaacttctc tttatcctgc tcgtaacgct ccgctttgaa accgccgccg tggattttgc
     3241 cccgcagttt atccggggga taggcgctcg cccccgcgaa ttcgatgaat tccagtttga
     3301 ccttttcccc ctcgttgatg cggaaggata agactacctc atcggcgctc ccttcggtcg
     3361 ccgatttgct ctcgacggcc gccaggtagt accctttctc ttcgcagagg gagcggatgc
     3421 ggttcacatt ttcctggacg gcgaccgggc tggcggtctg accttcccgt agcgtcaatt
     3481 tcgtccggat ctcttcggtt ttgactttct tgttgccggc aatgtcgatg cggcggattt
     3541 tggggtactc ctgaacctgg atgaccagag cgatcccatt gtttccctgg aggtcaccct
     3601 cgatggctac gtcggagaat aaattgagcc catagatgcg tcggatggcg tccgccacgc
     3661 cccccgcatc caaccggctg ccgatggcca gtccggccac gttctgaatc agcgaccggt
     3721 ccgctacccg gttgccctgc acctcaaccg agacaatctg atactcctga gtctgagaga
     3781 aagctgggag ggcccacagg cacacaacca cgagacgtgg tagccacgct ctctttagcc
     3841 tgagtcccgc ccagggcggg atcgaaggcc ggagcgtgga atttgcgcga tgccgatggg
     3901 gaacgttttt tctccagtta ataaaaaacc aaaccggccc gccctccccc tgcgctgccg
     3961 gtttatgcgt gcgctccttg cgtttcaaag gcttcatccc gtgtcctctg gcctgctgat
     4021 aatacgcagg ctcaggcaat ctggggagcc tgtattttat tgatttttac ggcggtgtac
     4081 cactggcggt gcccccgttt gcggcggtac ttggtccggc gtttgaactt aaagacgatg
     4141 attttctccc cgcgcgcctc ccgcaccact tcgcccaaaa cgctggcctt gggcaggtag
     4201 ggattcccga ccgcagtgcc tttttcgccg gccaccaaaa gcaccttgtc aaaagtcact
     4261 tggtcctcgg ccttggcccc cagccgggga acctcaacca cttcgccctc ccgcaccggg
     4321 aactgcattc cccctatctc gacgatcgca tacattcgct tccactcctt tgcttctcgt
     4381 aggttgtaag cattggctaa tatacacctt cccgatttcg gacgtcaagg gaccgaccag
     4441 cgtaaatttt tgacaagggt agggctcttg cgtatatgaa cggcctcgag aggactatac
     4501 cgatggttgg gatacggttc ggcgaaaaaa acgtgttgct tgtggtgggg ctggctgctc
     4561 tggctgttgc cgcctggacc gcttttgcct cttcgggtac ctgggaagac gatgcctaca
     4621 tcttctttca gtacgccaaa aatctggctg ccggtcaggg aatggctttc aatagcggcg
     4681 aagtaagctt cggtgtcacc agcgtgctct ggacgctcat tttgggtttc ctcagctggg
     4741 tcttccacct gggcccggtg gtgatggcca aactcgcatc catagtcctg ttttctgcct
     4801 cggtgaccat cctcacccga ttggtgatcg actgcacaca gaactctctg ctcgggttct
     4861 ggacgggtct attggtcgcc gtctacccgc acctcgtgct ccatgctgtc agtggcatgg
     4921 aaatcccgct caaccttttt ctcttgactg tcctgactgt ctcgttcttc cgttatgggg
     4981 agaaacattt ctggctctac ggaatcctca tcggtcttgc cttcctcacc cgcccggaaa
     5041 atgccctctg gctgccgctg tttgtttggc tggcggcaac ttcccgggga cagttcggcc
     5101 tgagaatcgc ggccctcctg ggcacctgtg ttggagtcgt cctcccgtgg gaattgtacc
     5161 tctactccca cacctctctc tttctgccgc ctacccggga tggtaagctc ttgctcttcc
     5221 tgcccgtcca gtacggcgtg accctggacc agttccgtca cctgagcctc gcggcccgac
     5281 ttgatctggc tggccgggca tcggtgaatg tcgccaaggc gatctttctc aacaaaggtg
     5341 gtgtactgtt tctccccttt ctcctagtgg ccggttactt cgtggctcgc gggaaaattg
     5401 ctctggggcg attcgggctg gcactggcgg ggtacggctt tggtctggtg gcactctttg
     5461 tccttttctt ccctctggtg aaacaacgct acttcgttca cctctatccg ttcttgattt
     5521 tcatctcggt tttgaccggc tgccgcctgt ggcaggaact ccgtgggaga ttcaaaatcc
     5581 tcatccgccc gctctgggcc aaagtcgggc ttgtcactgt cctgctcatc gcgccgttgg
     5641 ccggattctt cgccgcgcga aagtttgcca gtatgtcccg ggagcagagc attcgggcgg
     5701 aagtcgggcg ctggctcaaa accaataccc cgcctgacgc ccgcgttgcg ctcgaaccga
     5761 tcggcgcggt tgcctatttc gccgatcggt acgtcgtcga tctcggagga ctcatcaacc
     5821 ctgacatctg gcctttcatg tcccgcggac cggattcgcc gccggatgca ctctttgaca
     5881 ttctcgtgag gaaaagggtg acccacgttg tcgattacag ccagcacccc tgggcaggga
     5941 aagtcgtcga tctctatccg gaaagattcc tactggctgc gcagatctcc tcgccttatc
     6001 ccccggcggg ctacaaccag tatgacattt ataaactgaa caaataaccc cgactggttg
     6061 ccgatcacct ccgcaaccgg cgtgcctgct ggtgaacaaa ttcacagccg cctgctctcc
     6121 ctttttcccc cgatatgccg atatttcatt gaggagcgtc cggcgcgaag ccgagagcga
     6181 aaaatcgctg tgggcacaaa aaaatatctt gtctccacgt gtgccggaca gtatattcgc
     6241 ccagtacgca gcgtggctgc gtatatcata tagagccccg ccttaggcgg ggcccaaaaa
     6301 cgttcaaagg attgaaaaac gatgaatgag atgttttccg aacgcgcccg gcgggccatt
     6361 gagtatgccc gcgacgaagc ggc
//
LOCUS       DBNGZA010000043         3914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1386277, whole genome shotgun sequence.
ACCESSION   DBNGZA010000043 DBNGZA010000000
VERSION     DBNGZA010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1386277"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="AC16FU_00940"
     CDS             complement(<1..830)
                     /locus_tag="AC16FU_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR2992976.1"
                     /translation="MKKRLWLKGSLVAAVWFLFIFALVPRGQAAPQPVDPGIRYQFSQ
                     VAEKVLPAVVTVRVEQTVKSQPFAGFPDDQSGDDFFHRFFGTPQGDTQSFRQHGMGSG
                     VIVSSDGFILTNNHVVGRADEISVSVSGRKEPLKAKVIGTDSPTDLAVIKVDAADLPA
                     AKLGNSDSVRIGEWVLAVGAPFQLEHSVTGGIISAKGRSRVGIADYEDFIQTDAAINP
                     GNSGGALVNLVGEVIGINTAIATQTGVYAGVGFAIPINMAKKIMQSLIAEGRVVRGFL
                     G"
     gene            complement(996..2273)
                     /locus_tag="AC16FU_00945"
     CDS             complement(996..2273)
                     /locus_tag="AC16FU_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR2992977.1"
                     /translation="MSHNKRAVKTVAVLSGFLVLFTFLGCGKRRNVVSSSSVAAGALQ
                     LSVHEFPSLKNNPLEAGNPPTDSRARRVIVYTPPGYRPVGFGPPFPVLYLLHQFDGNE
                     NSFGMYQLARVMDELIAAGEIQPMLVVQTDASTIYGGSFYTNSTGTGNYADMIVKNLI
                     DYIERDPIARYNVFGGRRSRAIGGHGMGGYGALRLALENDSLFSSVSCMSAPLAFDGT
                     GGDFKGFIDRLRFVDTMFAENGLLTGGQILVSGRAVVDTAEAFRRYKNLLRPDPGKPL
                     TRLFFAMAAALSPTQQGDKDSLKFKLTLKQADPGMTLPINFLGKVEQPIFDRWVAKDV
                     HTMVKNNPNKLDSLPVYVDAGNQDDFQFNLQSEAFNETVQLLKQAHPFYDYVFEEYAG
                     YPGVPANHTNFISDRLRQVLKFHSDRMLGPPKN"
     gene            complement(2325..3827)
                     /locus_tag="AC16FU_00950"
     CDS             complement(2325..3827)
                     /locus_tag="AC16FU_00950"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2992978.1"
                     /translation="MFGRVKRMWGILFFQKSKLTGNGRCGLRVACAALISLYLLAGAP
                     AWSSGFALSGVGVKASGMGGAFRAVADDWSAAYWNPAGLAYVTQSEVIVLGQVFSPRP
                     DFTPNVTYNGYEVGYGSVNGTRQFPNDRNFLIGGVSGFYRIPTIEAFTFGVAVFEPHT
                     YASEWNLYKLPYPFKGDSSFAFPEKNFQSKIKVVDIHPTIAKEIIPGRLALGLGIGVE
                     WGTLHLRQPFFQSNPFPPPLNSRPEEFFVVDNRLRTSGWGVGANFGALVKIGSVLRMG
                     ASLRSPVTLHLNGDLTSTTYFPFNLAKMQDTLVPASQKFMFGGQSRRFSGAAEGKLNL
                     PWDIGGGVAVFLGKRLTFSADAAYTRWANLWNLQFAVSGQGLLGQPLAPLSMPLHWEN
                     TWRGSAGVQVVASDYLTFRGGYHFEGSAIPDQTFSPLFVDLDNKHSVNAGAEAALGGF
                     GFGYSFEWLFASRRNVSNLADVNGDGMYDNLTGTYDFSAFVSYATVSYRF"
BASE COUNT          858 a         1138 c         1142 g          776 t
ORIGIN      
        1 cccagaaaac cgcgcaccac ccggccctcg gcaatcaaac tctgcatgat ttttttggcc
       61 atgttgatgg ggatggcaaa accgactccc gcatagacgc cggtctgggt ggcgatggcg
      121 gtgttgatcc cgatcacttc accgactaag ttcaccagcg cgccgccgga attgccgggg
      181 ttgatggccg cgtcggtctg gatgaaatct tcgtagtcgg caatccccac ccgcgagcgg
      241 cccttggcgg aaatgatgcc gccggtcacg ctgtgctcca gctggaaggg ggcgccgacc
      301 gccagcaccc attcgccgat gcgcaccgaa tcggaattgc ctaattttgc tgccgggaga
      361 tcagccgcat cgactttgat gaccgctaag tcggttgggc tgtccgttcc gataactttg
      421 gctttgagcg gttccttgcg cccggagacc gagaccgaaa tttcatctgc cctgcccacg
      481 acgtggttat tggtgaggat aaagccatcg gaggagacga tgacacccga ccccatgccg
      541 tgttgccgaa agctctgggt gtccccctgg ggggtgccga agaagcggtg aaaaaagtcg
      601 tcgccggact gatcgtccgg aaagccggca aagggctggc tcttgaccgt ttgctcgact
      661 cggacggtca ccaccgccgg cagcactttt tccgcgacct gggaaaactg gtagcggatt
      721 cccgggtcga ccggctgggg tgccgcctgc ccgcgaggaa ccagtgcgaa aatgaaaaga
      781 aaccacaccg ccgccacaag agaaccttta agccataatc tcttcttcat cgtaggtacc
      841 ttcctttaac cctttcccac agcctgacgc ttccagtatt tcttatacgt acagagttcc
      901 cggccggttg accctctttc accacggcgg gaagtacggt ccgatgaaaa aaccggggca
      961 ggttgagccc cggtcggtag gcggttgtgc aacgtttagt ttttcggcgg accgagcatc
     1021 cgatccgagt ggaacttgag cacctggcgc aggcggtcgc tgatgaaatt cgtgtggttg
     1081 gccggcacac ccgggtagcc ggcatactct tcaaagacgt agtcgtaaaa aggatgggcc
     1141 tgcttcaaga gttgcaccgt ttcgttgaag gcctcagact gcaggttaaa ctggaaatca
     1201 tcctggtttc cggcatcgac gtagaccggc aagctgtcca acttgttggg attatttttc
     1261 accatcgtgt ggacatcctt ggctacccac cggtcgaaga tcggctgttc caccttgccc
     1321 aaaaaattta tcgggagggt catgccggga tcggcctgct ttaaggtcag tttgaacttc
     1381 agactgtctt tatcgccttg ctgggtcggc gacagcgcgg cagccatagc gaagaacaaa
     1441 cgggttagcg gctttcccgg gtccggccgc aggagatttt tatagcgacg gaatgcttcc
     1501 gccgtatcta cgaccgctct accggagacg aggatttgac cgcccgtcaa cagcccgttc
     1561 tctgcgaaca tcgtgtcgac gaaccgcagg cggtcgatga atcctttgaa atcgccgccg
     1621 gtaccatcga acgccaaagg cgcgctcata caggaaaccg aagagaagag agaatcgttt
     1681 tccagcgcca gccgcagggc gccgtagccg cccataccgt gcccgccgat ggcccgcgac
     1741 cgccgtccgc cgaacacgtt atagcgggcg atcgggtccc gttcaatgta atcgatcagg
     1801 tttttgacga tcatgtcggc gtagttgccg gtgccggttg aattggtgta gaaactcccc
     1861 ccgtagatgg tggaagcgtc ggtctgtacc acgagcatgg gttgaatttc ccccgctgca
     1921 atcagctcat ccatcacccg ggccagctga tacatgccaa aggagttttc attgccgtca
     1981 aactggtgga gcaggtagag caccggaaag ggcggtccga aacccaccgg ccggtatcct
     2041 ggcggggtgt agacgatgac ccggcgcgcg cgcgagtcag tgggcggatt cccagcttcc
     2101 agtggattat tcttgagcga aggaaattca tgcacggaga gctgcagggc accggccgct
     2161 accgacgagg aggagaccac gttccggcgc ttgccgcaac ccaaaaaggt aaagagtacc
     2221 aggaaaccgc ttagaaccgc cactgttttc actgcccgtt tattatggct catgctgaac
     2281 tccctttgcc gttcgcgatt ttcaactttt ctggtcagaa gaggttagaa ccggtagctg
     2341 acggtagcgt acgaaacgaa agcggaaaaa tcgtacgtgc cggtcaagtt atcgtacatc
     2401 ccgtcgccgt tcacgtcggc cagattggag acgttccgcc ggctggcgaa gagccactca
     2461 aagctgtagc caaatccgaa cccgcccaga gctgcttccg cccctgcgtt cacactgtgt
     2521 ttgttgtcca gatcgacgaa aagaggcgag aaggtctggt cgggaatcgc cgaaccctcg
     2581 aagtggtacc ctccccggaa ggtgaggtaa tcggaagcca cgacctggac tccggcgctc
     2641 ccccgccagg tgttctccca gtggagcggc atggagaggg gggccaaggg ctggcccagg
     2701 aggccctgac cgctgacggc gaactgcagg ttccagagat tggcccagcg ggtgtaggcc
     2761 gcatcggcgg agaaagtcaa ccgtttgccg aggaaaacag caaccccgcc gccaatatcc
     2821 cagggcaggt tcagttttcc ttccgcggcg ccggaaaagc ggcgtgactg gccgccgaac
     2881 atgaacttct gggaagccgg caccagcgta tcctgcattt tggcgaggtt gaagggaaaa
     2941 taggtcgtcg aggtgaggtc gccgttcaaa tgcagggtca ccggactacg gaggctggca
     3001 cccatgcgaa gcacgctccc gattttcacc agggcaccga aattggcgcc cacgccccag
     3061 ccgctggtac ggaggcggtt atccacgacg aagaactctt ccggacgact gttcaaaggc
     3121 ggcggaaagg gattggattg gaagaacggc tggcgcaggt ggagcgttcc ccactcgacg
     3181 ccgattccca gacccaacgc caggcgtccc gggatgattt ctttggcgat agtcgggtgg
     3241 atgtccacca ccttgatctt gctctgaaag tttttctcgg gaaaagcgaa cgaggaatcg
     3301 cctttgaagg gatacgggag tttgtagagg ttccattcac tggcgtaagt atgcggttcg
     3361 aacaccgcaa cgccaaaggt gaacgcctca atcgtcggaa tgcggtaaaa accggaaacg
     3421 ccgccgatta aaaaattccg gtcattcgga aactgccggg taccgtttac gctcccatac
     3481 cccacctcgt agccattgta ggtgacgttg ggggtaaaat ccgggcgagg ggaaaagacc
     3541 tgccccagga cgatcacttc cgactgggtg acatacgcca ggccggcggg attccagtac
     3601 gcggcactcc aatcgtcggc cacagcgcgg aaggcgccac ccatgccgct ggctttgacc
     3661 ccgactcctg ataaggcaaa tccggaactc catgccggtg cgccagcgag aagatagagt
     3721 gagatgagcg cggcgcaggc cactctcagg ccgcagcgac cgttccccgt cagtttcgat
     3781 ttttggaaaa aaagaatacc ccacatccgc ttaaccctcc cgaacatttg cctcattttc
     3841 tcttgccacg taatcattgc cgaacccgtt agaccccgca ctacgaaata gcgcgcaatt
     3901 aaacagatgc ccta
//
LOCUS       DBNGZA010000044         2131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1810349, whole genome shotgun sequence.
ACCESSION   DBNGZA010000044 DBNGZA010000000
VERSION     DBNGZA010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1810349"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC16FU_00955"
     CDS             complement(<1..372)
                     /locus_tag="AC16FU_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR2992979.1"
                     /translation="MDTTRTGTNLRYWTKQVTFVFGGLALFLLLNNLLAPQLDPYILR
                     LIILSGINIILAVSLNLINGFTGQFSIGHAGFMAIGGYCAAALTVYGGPSILAALSGL
                     GLPPVAARSILFLLSLLAGGLL"
     gene            complement(378..1280)
                     /locus_tag="AC16FU_00960"
     CDS             complement(378..1280)
                     /locus_tag="AC16FU_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2992980.1"
                     /translation="MQEFLQQVFNGIAWGSIYALIALGYTMVYGVLKLINFAHGDVFM
                     VGAFVGYYFSHWLGISDHPSLGGALAILFLAMALCALLGVIIERGAYRPLRKQPRLTV
                     LITAIGVSLLLENGGQLVFGANPKFFPSLIADRELVNTGGVVIRLLPVVVLAVSLVLM
                     LFLRFIVVKTKIGKAMRAVSYNREAASLMGIPTDNVITFTFILGSALAGAGGVLVAST
                     YPKIDPLMGLLPGLKAFVAAVLGGIGNIPGAVLGGMILGVSETMVAGYISSTLRDAIA
                     FGLLVFILLVKPTGLLGSPVSEKV"
     gene            complement(1284..>2131)
                     /locus_tag="AC16FU_00965"
     CDS             complement(1284..>2131)
                     /locus_tag="AC16FU_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR2992981.1"
                     /translation="GEVASTRSMAGGRICQQNKIPMVSPSSTNPAVTQIGDYIFRVCF
                     IDPFQGEVMAIFCRNTLKMKRVAILKDVKNDYSVGLSEYFAKTFKESGGQVVAEEAYT
                     EGDQDFKAQLTTIKTKRPETIFVPGYYTEVGLIARQARELGITVPITGGDGWESPDLL
                     KVGGAALDNCYFSTHYSSDDPRSEVQNFVATYKQKYGQAPDAIAPLAYDAARILFAAI
                     QKANSTDGDAVRRVLAETKDFSGVSGQITIDANSNAVKPAVVLKIEHGQYKFVEQIAP
                     GTPAP"
BASE COUNT          485 a          578 c          672 g          396 t
ORIGIN      
        1 gagcaggccg cccgccagaa gactcaagag aaacaaaatt gagcgcgccg ccaccggggg
       61 cagccccagt ccggagagtg cggccaaaat tgacgggccg ccgtaaacgg tcaaagccgc
      121 cgcacagtag ccaccgatgg ccataaagcc ggcgtggccg atggaaaact ggccggtgaa
      181 gccgttaatt aaattcaaac tgaccgccag gataatgtta atgccggaga gaatgatcag
      241 tcgaaggata tagggatcga gttgcggtgc caggaggttg ttaaggagca aaaagagggc
      301 caggccgccg aaaacgaaag tgacctgttt ggtccagtag cgcaggttgg tcccggtacg
      361 agtcgtgtcc aacacggcta cactttctcg ctcaccgggg aacccaaaag tccggtcggt
      421 ttcaccaaga gaatgaatac taaaagaccg aaagcgatgg cgtcgcgcag ggtcgaggag
      481 atgtagccgg cgaccatagt ttccgacacc cccagaatca tcccgcccag aacggcgccg
      541 ggaatgttgc cgatgccgcc taaaacggcg gcgacaaagg ccttgagtcc cggcaaaaga
      601 cccattaagg ggtcaatttt ggggtaggtt gaagccacca agacgccgcc cgccccggcc
      661 agggcggagc ccagaatgaa ggtaaaggta atcacgttgt cggtgggaat gcccatgaga
      721 ctcgcggctt cgcggttgta cgataccgcg cgcatcgctt tgccgatttt ggttttgacg
      781 acgatgaaac gcaagaagag catcagtacc aaagagaccg ccagcactac caccggcagg
      841 aggcgtatta ctacgccgcc ggtgttcacc agttcgcggt cggcaatcaa cgatggaaag
      901 aattttggat tggcgccaaa gaccagttgg ccgccgttct ccagaaggag cgagacccct
      961 atggcggtga tcagaaccgt cagtcgcggc tgcttgcgta gcgggcggta ggcgccgcgc
     1021 tctatgatca cgccgagaag ggcacagagg gccatggcca gaaagaggat cgccagggca
     1081 ccccccaggg aggggtggtc ggagatgccg agccagtggg aaaaatagta gccgacaaaa
     1141 gcgccgacca taaagacgtc gccgtgggca aagttgatca acttgagcac accgtagacc
     1201 atggtgtacc ccagagcgat caaggcgtaa atggagcccc aggcaatgcc gttgaagacc
     1261 tgctggagga attcctgcaa gggtcagggg gccggtgtgc cgggagcgat ctgttcgacg
     1321 aacttatact ggccgtgttc gattttgagc accaccgccg gctttaccgc attgctgttg
     1381 gcgtcgatgg tgatttggcc ggaaacgccg gagaaatctt tcgtttcggc caggacccgg
     1441 cgaacggcgt cgccgtcggt ggagttcgct ttctggatgg cggcaaagag aatgcgggcg
     1501 gcgtcgtagg ccagaggcgc tatggcgtcc ggcgcctggc cgtacttctg cttgtaggtg
     1561 gcgacgaagt tttggacttc cgaacgcggg tcgtcgctgg agtagtgagt ggagaagtaa
     1621 cagttgtcga gcgccgcacc ccccaccttg agcaggtcgg gcgactccca gccgtcgccg
     1681 ccggtaatgg ggacggtaat ccccagctcc cgcgcctggc gggcaatcag gccgacttcg
     1741 gtgtagtagc cgggcacgaa aatcgtttcc ggccgtttgg ttttgatggt ggtcaattgc
     1801 gctttgaaat cctgatcgcc ttcggtgtag gcttcttcgg cgaccacctg gccgcctgac
     1861 tctttgaagg ttttcgcaaa gtactcggat aatcccacgc tgtagtcatt cttcacgtct
     1921 ttcaagatag ccaccctttt cattttgagc gtattgcggc agaatatagc catcacttcg
     1981 ccctggaacg ggtcgatgaa gcaaacgcgg aaaatatagt cgccaatctg ggtgaccgcg
     2041 gggttggtgg aagaggggct gaccatcgga atcttattct gctggcagat gcggccgccc
     2101 gccatgctcc gggtgctggc cacctccccc a
//
LOCUS       DBNGZA010000045         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_644701, whole genome shotgun sequence.
ACCESSION   DBNGZA010000045 DBNGZA010000000
VERSION     DBNGZA010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_644701"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC16FU_00970"
     CDS             <1..523
                     /locus_tag="AC16FU_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR2992982.1"
                     /translation="TFPQLVAEKFGEEFSRKAQEIFWKMSVVEDAITAASVGTRKKGV
                     TAMHDATECGVWGGLYEVAQASGVGMRVEKDKIVVLDEVEKICSLFGMDPYASISEGT
                     LIITAVAGKAEKIVAALRRKKIDAAVIGEVTKKSLGMVIKEGGREHRLEHPRVDPFWE
                     AFGRTLARVQGKT"
     gene            680..1843
                     /gene="nifS"
                     /locus_tag="AC16FU_00975"
     CDS             680..1843
                     /gene="nifS"
                     /locus_tag="AC16FU_00975"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR2992983.1"
                     /translation="MRPVYLDHNATTPVLPEVAEAMTQVFVNAFGNASSIHTFGREAK
                     GYLEEAREQVAKFIGAAPSEVYFTSGGTEADNLALKGVSNAYREKGKHIITSQIEHHA
                     VIHSGQHLSEEGFEVTYLPVDRYGLVDPDDLRKAIRPDTILVSIMQANNEVGTIQPIS
                     ELAEIAHAKGVLFHTDAVQALAKIPVNVNELGVDLLSISAHKVYGPKGTGALFIRKGI
                     DITPLLHGGSHERNRRAGTENVAGAVGFAKALEIAERDGEQERARQNTLAEYFRTEVE
                     KRIEDSVFNGHPTRRVPTTCNFSFKYVEGEAIIVNLDLEGIAVASGSACASGATEPSH
                     VLTAMHIHPDIAKSSVRFSLGRSNIREDIDYVLDVLPQVVQRLRAMSPLYVRT"
     gene            complement(1937..>2456)
                     /locus_tag="AC16FU_00980"
     CDS             complement(1937..>2456)
                     /locus_tag="AC16FU_00980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2992984.1"
                     /translation="PAAFRITNQKFSKVQGDVVNPEQVEAAIAGKEAVLSALGFSRNS
                     PKTVCADGTRNMIAAMQKHGVRRLICESAYGVGDTRNRGFYARFLSLIIQSRLADKEK
                     MEAAVRQSGLDWVIARPTALTDGSKTGGYRAGSNLQLGFWPMISRADVANFMLKQITD
                     TTFLRQSPTVSY"
BASE COUNT          556 a          686 c          726 g          488 t
ORIGIN      
        1 aacctttcct cagttagtag ccgagaagtt cggggaagag ttcagccgta aggcgcagga
       61 gattttctgg aagatgtcgg tggtggagga tgctatcacg gcggcttcag tcggaacccg
      121 caaaaaaggc gtcaccgcca tgcacgacgc caccgagtgc ggcgtctggg gtgggctcta
      181 cgaagtagcc caggcctccg gcgtggggat gcgcgttgag aaggacaaaa ttgtggtatt
      241 agatgaagtg gagaagattt gctccctctt cgggatggat ccgtacgcct caatttccga
      301 agggacgctc atcatcactg ccgtcgccgg gaaagcagaa aagattgtgg cggccttgcg
      361 gcgcaagaaa attgacgctg ccgtcatcgg cgaggtgacg aagaagagtt tgggaatggt
      421 gataaaggaa ggagggaggg agcaccgctt ggagcacccc cgcgttgatc cgttctggga
      481 ggcgttcggc aggaccctgg cgcgagtgca agggaaaacc tgattcagcc gagcactcag
      541 atatattagt tcgagggtac cgaaaagacg cccgcacttc aaattgcagc gcgcggttga
      601 gattgccggg acaaaattag tttggtatct gagggggagg tgcgtatatt taatgaaggg
      661 attttgaggt ttccccgaga tgcgccccgt ctacttagac cataacgcca ccacgccggt
      721 cctgcccgaa gtcgccgagg caatgacgca ggtctttgtc aacgcattcg gtaacgcctc
      781 ctctatccac acctttggcc gggaggccaa gggctacctc gaagaggcgc gggagcaggt
      841 ggcgaaattc atcggggctg cgccttccga agtctacttc acctccgggg gcacggaggc
      901 ggacaattta gccctcaaag gcgtttccaa cgcttatcgg gaaaaaggca agcacattat
      961 cacctcccag atcgagcacc atgccgtaat ccacagcggc cagcacctct ccgaggaggg
     1021 gttcgaggtc acctacctgc cggtggaccg ctacgggctg gtcgatccgg atgacctgcg
     1081 gaaagccatc cgcccggaca cgattttggt ctcgatcatg caggcaaata acgaagtcgg
     1141 caccattcag ccgatttccg agttggctga aatcgcccac gccaaaggag tcctctttca
     1201 caccgacgca gtgcaagcgc tggccaagat tccggttaac gtgaacgaat taggcgtcga
     1261 tttgctctca atctcggcgc acaaggtcta cggcccgaaa gggaccggcg cgcttttcat
     1321 tcggaagggg attgacatca caccgcttct gcacggaggc agccacgagc gcaatcgccg
     1381 ggcggggacg gaaaacgtcg ccggtgcggt gggttttgcg aaagcgctgg aaattgcgga
     1441 gcgtgacggg gaacaggaac gggcgcgtca gaatacatta gccgaatact tccgcaccga
     1501 agtcgaaaag cgcatagagg actcagtctt caacggccat cccacccggc gggtgcccac
     1561 cacctgcaac ttttcgttca agtacgtcga aggcgaagcg attatcgtta accttgactt
     1621 agaagggatc gccgtggcct ccggttcggc ctgcgcatcg ggcgcgacgg aaccatcaca
     1681 cgtcctcacc gcgatgcaca ttcaccccga catcgccaaa agttcagtgc gcttctccct
     1741 gggccgttct aatatccgcg aagacatcga ctacgtgtta gacgttctgc cgcaggtcgt
     1801 ccagcgcctc cgggcgatgt caccacttta cgttagaacg taatcaggtc agaaaaaacg
     1861 ttacccagac aacgaaatcg gaattcccca tagggcgacc gtctcggtcg cccgaaagtg
     1921 agctgcccat ccagttctaa tagctcaccg tcggtgactg ccggagaaat gttgtatcgg
     1981 tgatttgttt gagcatgaaa ttggccacgt cggcccgcga aatcatgggc cagaagccga
     2041 gttgcaggtt tgatccggcc cggtagccgc cggtctttga accatcggtc aatgcagtcg
     2101 gacgcgcgat tacccaatcg aggccgctct gacgaacggc cgcttccatt ttctctttgt
     2161 cagccaggcg actctggata atgagcgaga gaaaccgggc gtagaaaccc cggttccggg
     2221 tgtcacccac gccgtaggcc gactcgcaaa tcaagcgccg cacgccgtgc ttctgcatcg
     2281 ccgcgatcat gtttcgggtg ccgtcggcgc aaacggtttt cggagaattt ctgctgaaac
     2341 ccagagcgga gaggaccgcc tcttttcctg caatggctgc ttccacctgt tcgggattca
     2401 ctacatcacc ctgaaccttc gagaatttct ggttggttat gcgaaaggca gccggg
//
LOCUS       DBNGZA010000046         2210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1704395, whole genome shotgun sequence.
ACCESSION   DBNGZA010000046 DBNGZA010000000
VERSION     DBNGZA010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1704395"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1582)
                     /locus_tag="AC16FU_00985"
     CDS             complement(152..1582)
                     /locus_tag="AC16FU_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2992985.1"
                     /translation="MNPRARILVVDDEPLIREFLNETLSRSGFLVEQAAAGEEAWQKI
                     QSASGGYDVVFTDVKMPQMNGLELLRTVKAHNPDVSVVVLTGYGSIADAVAAIKLGAF
                     DYLTKPCTPAQIEHAVQKALEFQRLVAENRQLRQELVSRYQYANIVGKSALMQKVFAL
                     VDTVASSRATVLITGESGTGKELVAHAIHYNSPRREGPFVKLNCAALPEGLIESELFG
                     HERGAFTGAIRSARGRFERADGGTLLLDEISEMPLGLQAKLLRVLQEREFERVGSGDT
                     IQVDVRIVATTNRNLSAVIREGKFREDLYFRLNVVNIPIPPLRDRLEDLPYLVNHFIR
                     KYNEENGKKIEGISEKVLAVFSRYHWPGNVRELENYLERAVVLSPGPLLQPSDFPPEV
                     TLGKITAPTDGVKIGMSLHDTEKLMILKTLESQAGNRTKAAEVLGISTRTLRNKLEEY
                     WLKEYGAAWGPRGVIRFGRGAQLVSP"
     gene            complement(1579..>2210)
                     /locus_tag="AC16FU_00990"
     CDS             complement(1579..>2210)
                     /locus_tag="AC16FU_00990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2992986.1"
                     /translation="GGIAGFAAILDHDLPENDPRKPLIGKILAGIEQLNRMAEGLLHH
                     ARGPEIRPSRFDAVQFLEDIARQCEAELQSAGRRVILRRDFSAKTLPVHLDPEYFRQA
                     VTNLLANSFQAVGEEGEVRVSANLEPSEPPTPRKTLVVKVQDNGPGMTPELLQKIFTP
                     FFTTKTDGSGLGLAIVRQAAQAHGGEVAVTSAPGKGATFTLRIPQDVQR"
BASE COUNT          398 a          698 c          659 g          455 t
ORIGIN      
        1 attccccgtc tcccggtcgt ggaactgccc ggggaagcgc acctcattga gcgtgttggc
       61 ctcctgaatc acatttccga acgggtctaa atcgacggaa taggttattg ctttcgatgt
      121 tccagtcatg gataaaggcg tctccaaatg gtcaaggtga taccagttgt gcacctctcc
      181 caaaccggat gaccccccta gggccccacg ccgctccgta ctctttcaac cagtattcct
      241 cgagtttatt ccttagcgtg cgggtggaaa tccccaagac ttcggcggct ttggtgcggt
      301 tgccggcctg cgactccagg gtctttagaa tcatcaactt ttccgtgtcg tggagactca
      361 tgccgatttt caccccgtcg gtgggcgcgg tgatcttccc cagggtcact tccgggggga
      421 agtcggacgg ctgaaggagc gggccggggg agagcaccac cgcccgctcg aggtagtttt
      481 ccaactcccg cacgttgccc ggccagtggt agcgggagaa aaccgcgagc actttttcgg
      541 aaatcccttc gatctttttg ccgttctcct cgttgtattt gcgaatgaag tggttcacca
      601 ggtagggaag atcctccagc cggtcgcgca ggggcgggat gggaatgttg acgacattga
      661 ggcggaaata gaggtcctcg cggaacttcc cctcgcggat gaccgccgaa aggttccggt
      721 tggtggtggc cacgatccgc acgtcgacct gaatggtatc gccggagccg acccgctcga
      781 actcccgctc ctgcagcacg cgcaagagtt tcgcctgcag gcccaagggc atctcgctga
      841 tttcatcaag cagaagcgtc ccgccatcgg cccgctcgaa ccggccccgg gccgagcgga
      901 tggccccggt gaaggcgccc cgctcgtggc cgaaaagttc gctctcgatc agcccctccg
      961 gcagcgcggc gcagttgagc ttgacgaacg gcccctcccg gcgcggggaa ttgtagtgaa
     1021 tggcgtgcgc cacgagctcc ttgccggtcc ccgactctcc ggtgatgagc accgtggcgc
     1081 ggctggaggc caccgtatcg acgagcgcaa agactttttg catcaaagcc gattttccga
     1141 cgatgttggc gtactggtag cggctgacca gttcctggcg cagctgccgg ttctccgcca
     1201 ccaggcgctg gaattccagg gctttttgga cggcatgctc gatctgcgcc ggcgtgcagg
     1261 gtttggtcag gtaatcgaag gcgcccaatt tgatcgccgc caccgcatcg gcgatgctcc
     1321 cgtagccggt caaaacgacc accgacacgt ccgggttgtg cgccttgacc gtccgcaaaa
     1381 gttccaaccc gttcatctgg ggcatcttca cgtcggtgaa gaccacgtcg tatccgcccg
     1441 aggcggactg gattttctgc caggcctcct cccccgccgc cgcctgctct accaaaaatc
     1501 cggagcggga aagagtttcg ttcaagaact cgcggatgag cggttcgtca tccacgacca
     1561 agatgcgcgc ccggggattc accgttgtac gtcctgcgga atgcggaggg taaaggtcgc
     1621 tccctttccg ggggcggaag tcacggcaac ctccccgccg tgggcttggg ccgcctggcg
     1681 gacgatcgcc agccccaggc cggaaccatc tgtttttgtt gtgaaaaaag gggtgaaaat
     1741 tttttgcagt aactcggggg tcatccccgg tccgttgtcc tggactttga ccaccagcgt
     1801 ttttcgcggg gtcggcggct ccgaaggttc caggttcgcg gaaaccctca cttccccctc
     1861 ctcgcccacc gcctggaaac tattcgccag gagattggtc accgcctgcc ggaagtactc
     1921 cggatcgaga tggacgggga gcgtttttgc ggaaaagtcc cgccgcagaa tcacccggcg
     1981 ccccgccgac tgcagttcgg cctcacactg gcgggcaata tcctccagaa attgcacggc
     2041 gtcgaagcgg gacggcctga tctccggtcc tctggcgtga tgcaacaggc cctccgccat
     2101 ccggttcaac tgctcgatac ctgccagaat tttcccgatc aagggcttgc gcggatcatt
     2161 ctccggcagg tcgtggtcta agatcgccgc aaaaccggcg atgccgccta
//
LOCUS       DBNGZA010000047         2821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2022818, whole genome shotgun sequence.
ACCESSION   DBNGZA010000047 DBNGZA010000000
VERSION     DBNGZA010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2022818"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2390
                     /locus_tag="AC16FU_00995"
     CDS             <1..2390
                     /locus_tag="AC16FU_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2992987.1"
                     /translation="VPIYVRNIGTVQLGGDIRRGVLDKNGVGEVVGGIIVMRYQENAQ
                     EVIKRVKAKIKEIAPGLPPGVKIVPFYDRSDLIHHAVSSLEHTLVEEAIIVSLVILIF
                     LLHPRSAFVVIISIPIAVLVAFISMYYLRITSNIMSLGGIAVAIGVLIDAAIIMVENA
                     YRHLADLPSGERREITPVVVRAAQQVGRPIFFSLVIIILSFLPVFLLEGQEGRLFRPL
                     AFTKTTAMAGAAILSITLVPVMMTFFLRGKLKPESANPVSRFFIKIYEPVLRLTLKHK
                     WKTLLINALVLVFTVFLTTRIGREFMPPLDEGSLLFMPITLPSVSITEAKRIVQIQDK
                     IIKEFPEVEAVLGKIGRAETATDPAPVSMIETIVLLKPKDQWRKGMNRVEIINELDRK
                     LQIPGVSNAWTMPIINRINMLSTGVRTDLGVKVFGSNLNIIEALGVEVEAALRKIPGA
                     VDIVAERVTGGKYLDIEIDRTRAARYGLNVGDIEEVIETAIGGERLTTTVEGRARFPV
                     RVRYLREYRDDPEKLKRVFVPTPLGQQIPLEQLANIKVTVGPPEINSENSLLRGIVFL
                     NVRGRDMGGFVDEAKRVLSASIKLPPGYFLSWSGQWENQVRAKKRLEILMPAVIFIIF
                     ILLYFTFHSVLEAFLVMLSVPFSLTGGVLLLWLLGYNFSVAVWVGFIALYGVAVETGV
                     VMVVYLHEALDRRLQRGTVDEAGLQEAIMEGAVLRLRPKLMTVFAALAGLLPIMWSSR
                     TGSDVMRPIATPMIGGMITSAIHVLIVTPVIFGIMKRHELRKGRLKPSGITLHHD"
     gene            2473..>2821
                     /locus_tag="AC16FU_01000"
     CDS             2473..>2821
                     /locus_tag="AC16FU_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992988.1"
                     /translation="MPKPLTPVPAPRRIPTAADSVVKIGSLRLSFSLYLWQDFMPIVP
                     PGGPPFHLSLEVTVKNQGTKPVTDFRLEKLSLYYDKSRRLFWTFRLASADSASGTLSI
                     APGAKRTFAYGPQS"
BASE COUNT          558 a          822 c          822 g          619 t
ORIGIN      
        1 gcgtgccgat ctacgtgcgc aacatcggca cggtgcagtt gggcggcgac atccgccgcg
       61 gcgttttgga caaaaacggg gtgggcgaag tggtgggcgg catcatcgtc atgcgctacc
      121 aggagaacgc gcaggaggtg atcaaacggg tcaaagccaa gatcaaagag atcgctccgg
      181 gcctgccgcc gggggtaaag atcgtgccgt tctacgaccg ctccgatttg atccaccacg
      241 ccgtgtcttc cctggagcac accctggtgg aagaggcgat catcgtcagt ctcgtgattt
      301 tgatcttcct gctccacccg cgctcggctt ttgtcgtcat catttcgatt ccgattgcgg
      361 tcctcgtcgc cttcatttcg atgtactacc tgcgcatcac gtccaacatc atgtctctgg
      421 gcggtattgc cgtggcgatc ggtgttctga tagacgccgc cattatcatg gtggagaatg
      481 cctatcgaca tctggccgat ttgccgtccg gagaacgtcg ggagatcacg ccggtggtgg
      541 tccgggccgc ccagcaggtg ggccggccga tcttcttctc gttggtcatc atcatcctct
      601 cgtttttgcc ggtcttcctt ttggaagggc aggagggccg gctcttccga ccgctggcat
      661 tcacgaaaac gaccgccatg gccggagcgg cgattctctc catcacctta gtgccggtga
      721 tgatgacctt ctttctccgg ggcaaactga agccggagtc ggccaatccg gtctcgcgct
      781 ttttcatcaa aatctacgag ccggtgctgc gcttgaccct caagcacaag tggaaaacgc
      841 tcctcatcaa cgcgctcgtc ttggttttca cggtcttttt gaccacgcgc atcggccggg
      901 agttcatgcc gcctttggac gaagggtcgc ttttgttcat gccgattacg ttgcccagcg
      961 tctcaatcac cgaggccaag cggatcgtcc agatacagga caaaatcatc aaagaatttc
     1021 cggaagtaga agcggtgctg ggcaaaatcg gccgggcgga aaccgccacc gatccggcgc
     1081 cggtctccat gatcgaaacg attgtgctct tgaagccgaa agaccagtgg cggaagggaa
     1141 tgaaccgggt cgaaattatc aatgaactcg accgcaagct gcaaattccc ggcgtttcca
     1201 atgcctggac gatgccgatc atcaaccgga tcaacatgct ctccaccggg gtgcggaccg
     1261 atctgggcgt caaggtcttc gggtcgaatc tgaatatcat tgaagccctg ggagtggagg
     1321 tcgaagcggc cctgcgcaaa attcccggag cggtggacat cgtggccgag cgggtgaccg
     1381 gcgggaagta cttagatatc gagatcgacc gtacccgagc ggcgcgctac ggcctcaacg
     1441 tcggcgacat tgaagaggtg atcgaaacgg ccatcggcgg cgagcggttg actaccacgg
     1501 tggaaggccg ggcgcgcttt ccggtgcgag tgcgctacct gcgggagtac cgcgacgacc
     1561 cagagaaact gaagcgcgtc ttcgtgccga cgcctctggg tcagcagatt cccctggagc
     1621 agttggccaa tatcaaggtc accgtcgggc cgccggagat caattccgaa aacagcctcc
     1681 tgcgcgggat tgtctttttg aatgtccggg gccgggatat ggggggattc gtggacgagg
     1741 ccaagcgggt gctatcggct tcgatcaagc tcccgcccgg ctactttctc tcttggagcg
     1801 gacagtggga aaaccaggtc cgcgccaaaa agcgccttga gatcctgatg ccggcggtga
     1861 tcttcatcat cttcatcctg ctctacttca ctttccactc ggtcctcgaa gcgttcctgg
     1921 taatgctctc ggtgcctttt tccctcaccg gcggcgttct gctcctctgg ctcttagggt
     1981 ataatttctc agtggcggtc tgggtcggct tcatagcgct ctacggtgtg gcggtggaaa
     2041 ccggcgtggt gatggtggtc tacctccacg aagcgctgga ccgtcgtctg caacggggaa
     2101 ccgttgacga agccgggctg caggaagcaa tcatggaggg ggcggtgctc cgcctgcggc
     2161 ccaaactcat gaccgtcttt gccgctttag ccgggctttt gccgattatg tggagcagcc
     2221 gcaccgggtc cgacgtcatg cggcccatcg ccacccccat gatcggcggg atgatcacct
     2281 ccgccatcca cgtcttgatc gtcacgccgg ttattttcgg cattatgaag cgccacgaac
     2341 tgcgcaaggg ccggctgaaa ccttcaggga ttacgctgca ccatgactaa aagacgctgg
     2401 gaaatagtcc tgctgttaac actggttttc ttcacgaccc cgcttttggg caaaacgaga
     2461 aaaggcgcat acatgcctaa accgctcacc cccgtgccag cacccaggcg gattccgacc
     2521 gcggcagatt cggttgtaaa aatcgggagc ttgcgactga gtttttccct ctacctctgg
     2581 caggatttca tgcccatagt accgccgggc gggccgccgt ttcatctctc actggaagtg
     2641 acggtcaaaa atcagggcac caagccagtt accgacttcc gactggaaaa actctcgctc
     2701 tattatgaca aaagtagaag gctcttctgg acgtttcgat tggcctcggc tgatagcgct
     2761 tcgggcactc tctctatcgc accgggggca aaaagaactt ttgcttatgg tccccaaagt
     2821 c
//
LOCUS       DBNGZA010000048         4461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_856571, whole genome shotgun sequence.
ACCESSION   DBNGZA010000048 DBNGZA010000000
VERSION     DBNGZA010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_856571"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC16FU_01005"
     CDS             complement(<1..284)
                     /locus_tag="AC16FU_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2992989.1"
                     /translation="MKLGRFEISHFVENRFHLDGGTMFGVVPKTMWSKLIPADERNLI
                     PMDENIFLVNAHGKNILLDVGLGDVLSERDRKVDNCYTPSCLEEGLRSLGV"
     gene            complement(342..1586)
                     /locus_tag="AC16FU_01010"
     CDS             complement(342..1586)
                     /locus_tag="AC16FU_01010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2992990.1"
                     /translation="MAEVQTLPKESVAEKTEPAKPRSRFDEIWGGGRPIWNWDPRQEA
                     VRRLGREFCEREVVPVAKKLDQVVPQQFAFDLYYKVAKAGFVGFSMPVEYGGGGRNSL
                     EYNTLVETLAYYDLSTCLLAAVGELATHPIIHFASEEQKRKYVPKCATGELISAYLLT
                     EPEAGSDAANQRTVAVPDGDHYVVNGEKIFIMHGDVAHLGVLFCRIQEEGARDKVSAL
                     IVDLTSPGVKRTTLQQKMGIRAATTGRVWFENVRVPKENLLGEKGRGFRYALATLDSA
                     RIGVAAQGVGMAQRAFDEAVGYAKKRIAFGNPIAKLQAIQWMIADMATRIEAARVLTY
                     KAALLQEQGVKFSLEGSEAKVCATETARFCVDRCMQIHGGYGYIGEFTEVEKLYRDQR
                     ILEIYEGTSEVQRLVIAKHYCG"
     gene            complement(1600..2949)
                     /locus_tag="AC16FU_01015"
     CDS             complement(1600..2949)
                     /locus_tag="AC16FU_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010900779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR2992991.1"
                     /translation="MPKGMRNVYMVSGGVSKFEKARPDVTFQPMVKEAYDYALKDLGL
                     EFKEASEVVDWTVASYFSDHFMRQLKAGAMVQDYLGLCPKPSHRVEGGGATGGLAFQE
                     AWKTVASGLAEVCVTYGWETMSHVNTWKGNEFIALASDTSFDYPVGGFYSGYYAMMVV
                     RHMHEFGTTVEQLAKIAIKNHKHAYYNPYSQKKIFDLSIKDVRNSPMVAYPLTRYDIC
                     VMSDGAAVAFLASEEGIKRIEKKTKRKIKPALVTGVGRGTDAMRFADRPREKVILLPH
                     EKASDYKDLEYPGVHSFRAGRMAGKLAYEMAGIGDPLNELDFIELHDAYSSSELQTYE
                     DLGLCKYGEGGKFVDAGYPFLPNLEYNFGEEQSSKQFKAKKRIPVNPSGGLIACGHPV
                     GATGLMQAVFALWQLQGTVKKHFKNDSLQVPNARRGIIHSHAGTGTYVTVSVMERVD"
     gene            complement(3042..3440)
                     /gene="mce"
                     /locus_tag="AC16FU_01020"
     CDS             complement(3042..3440)
                     /gene="mce"
                     /locus_tag="AC16FU_01020"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR2992992.1"
                     /translation="MFTRLDHIGIAVRDLDKAVAYWSELLELKSDHRIPVPAQGVEVV
                     FIPTRGARLELLAATGPDSPIAKFLERRGEGLHHVCFAVRDIEAELADLKSKGVELID
                     EKPRLGAEGKRIAFLHPKSTYGVLIELLEE"
     gene            complement(3454..>4461)
                     /locus_tag="AC16FU_01025"
     CDS             complement(3454..>4461)
                     /locus_tag="AC16FU_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR2992993.1"
                     /translation="FLYAKDRLPRWNTISISGYHIREAGSTAVQEVAFTLADAVAYVQ
                     TALEAGLAVDDFAPRLSFFFNAYNDFLEEVAKFRAARRIWAHLMREKFKAQKPDSWKL
                     RFHTQTAGSTLTAQQPENNIVRTTLQALAAVLGGTQSLHTNAYDEALALPTEKSVQIA
                     LRTQQIIAYESNVAATADPLGGSYYLEALTDAIETGVWKYLEQIEKAGGTLACVESGF
                     FQREIQRSAFAYQKKVEAGERVVVGVNRFTAKEAEQKNILKVDLRVQEEQMKSVRRLR
                     QERDSSKTEKARARLLAEAKGSSPLFEPILECVENYVTLGEICETLRSVWGEFRQQVI
                     L"
BASE COUNT          853 a         1363 c         1255 g          990 t
ORIGIN      
        1 acaccgagtg accgtaaacc ttcctcgagg caggacggtg tatagcagtt atcgactttt
       61 cggtcccgct ccgagagaac gtcacccagc cccacgtcca ggagaatatt tttcccgtgc
      121 gcgttgacca ggaaaatgtt ctcatccatc gggataagat tccgctcgtc ggcgggaatg
      181 agtttcgacc acatcgtctt gggcaccacg ccgaacatcg tgccgccgtc caagtggaag
      241 cggttctcga cgaagtggga aatctcaaaa cggcctagtt tcatcgaggg gggcaggcag
      301 tgccatcggc tcgagggaag gcgacggtag gaactctggc tctatccaca gtaatgcttg
      361 gcgatcacga gccgctgcac ctcggaggtg ccttcgtaaa tttccaagat ccgctggtcg
      421 cggtagagct tttcaacttc ggtgaactcg ccgatgtagc cgtacccgcc gtggatttgc
      481 atgcagcgat ccacgcagaa gcgcgccgtt tcagtggcgc agactttcgc ctccgagccc
      541 tcgagactga atttgactcc ctgctcctgc aggagcgccg ccttgtaggt caaaacgcgc
      601 gcggcttcaa tccgcgtggc catatcggca atcatccact gaatcgcctg cagcttggca
      661 atcggattcc cgaaggcgat ccgcttcttg gcgtagccca ccgcttcgtc aaacgcccgc
      721 tgggccatgc cgacgccctg cgccgccacg ccgatgcggg cgctgtctaa ggttgccagg
      781 gcgtagcgga acccgcgccc tttttcgcct aaaagatttt ccttcggcac ccgcacgttt
      841 tcgaaccaga cccggccggt ggtggcggcg cggatgccca ttttctgctg caacgtggtg
      901 cgcttgacgc ccgggctcgt cagatcaacg atcaaggccg agactttgtc ccgcgcccct
      961 tcttcttgaa tccggcagaa gagaacacct aagtgcgcca cgtcgccatg catgatgaaa
     1021 atcttctcgc cgttgacgac gtagtggtcg ccgtcgggga cagcgacagt cctttgattg
     1081 gccgcatcag agccggcttc gggttcggtc aaaagataag cgctgatcag ttcgcccgtg
     1141 gcacatttcg ggacgtattt ccgtttctgc tcctcgctgg caaagtgaat gatggggtgg
     1201 gtggccaatt cgcccactgc ggccagaagg caggtggaaa gatcgtagta cgccagcgtc
     1261 tccaccagcg tgttgtattc aagggagttt ctgccgccgc cgccgtattc aaccggcatg
     1321 ctgaacccga cgaaaccggc tttggcgact ttgtagtaca ggtcgaaggc gaattgctgc
     1381 ggtaccacct ggtcaagttt tttggccacc ggcaccactt cgcgttcgca gaactcgcgg
     1441 cccagtcgcc gcaccgcttc ctgacgggga tcccagttcc agatgggccg gccgccgccc
     1501 cagatctcat cgaaacgcga gcgcggcttg gccggttccg tcttctccgc cacagattct
     1561 ttcggcaaag tctgaacttc tgccatagcc agtcctttct cagtcaactc tttccatgac
     1621 cgaaacggta acataggtgc ctgtgcctgc gtggctgtgg atgattcccc gccgtgcgtt
     1681 cggcacctgc agcgagtcgt tcttaaagtg tttcttcacc gtcccctgca gctgccagag
     1741 ggcaaagacc gcctgcatca ggccggtggc gcccaccggg tggccgcagg cgatcaaccc
     1801 cccggagggg ttcaccggaa tgcgcttttt ggctttgaat tgcttgctcg actgctcctc
     1861 gccgaagttg tactccaggt tcggcaggaa ggggtagccg gcatcaacga acttcccgcc
     1921 ttcgccgtac ttgcagagcc ccaaatcctc gtaggtctgg agttcggatg aggagtaggc
     1981 gtcgtgcagt tcgatgaagt cgagttcgtt gagcgggtcg ccgattcccg ccatttcgta
     2041 ggccagtttc ccggccatcc ttccggcgcg gaaggaatga acgcccggat attcgagatc
     2101 tttgtagtcc gacgccttct cgtggggcag gaggatcacc ttttcgcggg gacggtcggc
     2161 aaaacgcatg gcatcggtcc cccggcccac gccggtaacc agcgccggct tgatttttct
     2221 tttggtcttc ttctcgatgc gcttgatccc ctcctcactg gccaagaagg cgaccgcggc
     2281 tccgtcggac atcacgcaga tgtcgtagcg ggtcagcggg taggccacca tcggcgaatt
     2341 acgcacgtct ttgatgctca aatcaaaaat ctttttctgc gagtaggggt tgtagtaggc
     2401 atgcttgtgg tttttgatgg caatcttggc caactgttcg acggttgtgc cgaactcgtg
     2461 catgtgccgc accaccatca tcgcgtagta gccggagtag aagcccccca cggggtaatc
     2521 gaaagacgta tcggaggcga gcgcgatgaa ctcattcccc ttccaggtgt tgacgtggga
     2581 catcgtctcc cagccgtagg tgacgcacac ctcggccaaa ccggaagcga ccgtcttcca
     2641 ggcctcctgg aacgccagcc cacccgtggc gccgccgcct tccacgcggt gcgacggctt
     2701 ggggcagagt cctaagtaat cctgcaccat cgccccggcc tttaactggc gcataaagtg
     2761 gtcggagaag taggaggcga cggtccaatc gacgacctcg ctcgcctctt tgaactccag
     2821 ccccaggtcc ttcaaggcgt agtcgtaggc ctctttgacc atcggctgaa aggtgacgtc
     2881 gggccgggct ttttcgaact tcgaaacccc gccggaaacc atgtagacgt tgcgcattcc
     2941 cttaggcatg aatcctcctg ttgttccggc cgaagataat agattttgta ttttggcgaa
     3001 acggtagcgc aggttggttt gcctaacttg ctccgccagc ttcactcttc gagtaactca
     3061 atcaagactc cgtaagtact ctttgggtgc aggaaggcaa ttctttttcc ttcagcccca
     3121 aggcgcggct tttcgtctat tagttcaacc cccttcgatt ttaagtccgc caactccgct
     3181 tcgatatccc tcaccgcaaa acaaacgtgg tgcagcccct cgccgcgcct ctccagaaat
     3241 tttgcaatgg gggagtccgg tccggttgcg gccaaaagct caagccgcgc tccacgcgta
     3301 ggaatgaaaa ccacctccac accctgcgcc ggaacgggga ttctgtggtc agatttcagt
     3361 tcaaggagct ctgaccagta agcaaccgct ttgtccaggt cacggacggc aatgccaatg
     3421 tgatcgagac gggtgaacaa gtttaactcg gactcagagg atcacctgct gccggaattc
     3481 cccccacacg ctgcggagcg tttcgcaaat ctcgcctaac gtcacgtaat tttccacgca
     3541 ttccagaatt ggttcaaaga ggggactgct tcctttggct tcagccagca ggcgggcgcg
     3601 ggctttttcg gtcttgctgc tatcgcgctc ctggcgcaac cggcggacac ttttcatttg
     3661 ctcctcctgc acccgcaggt caaccttaag aatatttttt tgttccgcct cttttgcggt
     3721 gaagcgattt acccccacca cgacccgctc ccccgcttcg actttcttct gataggcaaa
     3781 ggcgctccgc tgaatttccc tctggaaaaa acccgactcc acgcaggcca aggtcccgcc
     3841 cgctttttcg atttgctcga ggtacttcca gacgccggtc tcaatcgcat cggtgagcgc
     3901 ttcaaggtag tacgacccgc ccaaagggtc ggccgtggcg gcgacattgg attcgtaggc
     3961 gatgatctgc tgggtgcgga gcgcgatctg gactgatttt tccgtcggca gggccagcgc
     4021 ttcgtcgtag gcgttggtgt ggagcgactg cgttccgccc aagaccgcag ccaaagcctg
     4081 caacgtggtg cggacgatat tgttctccgg ctgctgggcg gttaaggtcg acccggcggt
     4141 ctgcgtgtga aagcgcaatt tccaggaatc gggtttttgc gctttgaact tttcccgcat
     4201 caggtgcgcc cagatccgcc gcgcggcgcg gaacttggcc acttcctcca gaaagtcgtt
     4261 gtacgcattg aagaaaaacg agagccgcgg cgcgaaatcg tcaaccgcca ggccggcctc
     4321 cagcgccgtt tgaacgtagg ccaccgcatc agccagcgtg aaggccactt cctgcactgc
     4381 cgtagacccc gcctcgcgga tatggtagcc ggagatcgaa atcgtgttcc agcgcggcag
     4441 gcggtctttg gcatagagga a
//
LOCUS       DBNGZA010000049         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1069793, whole genome shotgun sequence.
ACCESSION   DBNGZA010000049 DBNGZA010000000
VERSION     DBNGZA010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1069793"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16FU_01030"
     CDS             <1..539
                     /locus_tag="AC16FU_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2992994.1"
                     /translation="GSLKPWHGLEFLLEAFCRLPAREKLKLVIIGEGPLRHQLEKEAG
                     KLGLQDRVMFTGAVRFEQIPRTLKSLDAFVAPYPQIDGFYFSPLKIFEYMAAGRPIVA
                     SRIGQVAEILHDGKNALLVPPEDPAALVSALRRLKADRKLGARLGREAQRAALREHTW
                     QVRLATLEPVFHSLAAKR"
     gene            548..>2062
                     /locus_tag="AC16FU_01035"
     CDS             548..>2062
                     /locus_tag="AC16FU_01035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2992995.1"
                     /translation="MDLKFRIPLAKVDWVGLGRIFRHFGKHLGPHKKSLFWGGASLLG
                     LMLVELARPWSLKIVIDYILIPSKAKRDFGFLAPLSHWDPMAVLTFAAGSVAVLSLGG
                     ALFSYAQNVLFASVGEKVSGAIRLELFSHIQRLPQSYHDYRETGEVLMRLTGDIDLLK
                     ELLVSLFISLGSRIFIVVGMLALMLYMNWELTLIAIIVLPLLFLTSLRFSVKLKEASR
                     RQRKKEGQLAAAVYEGVSGISLTKIFGQEKRHDKLLGKSVSSDVKAGLRTTKLESAYA
                     RWVDVITALGVMLVLFFGVKQVQAGHLSAGNLLMFLSYLRSAYKPLRDVAQLSSRTAK
                     ATVCGERVLEILAMQPEVLDKPEAVPAQDIQGEIRFANVGFSYQTGRPVLSDLSFRLP
                     AGQTTAIIGPSGAGKSTIAKLLLRLYEPEAGTIFVDGVPIGDYQIKSFRKRITSLSQE
                     AFLFRTTIRDNISFGKPKASFDEIVAAARKVGADEFIRALPQGYDTLVGEGGQTL"
BASE COUNT          383 a          649 c          590 g          440 t
ORIGIN      
        1 tcggcagcct gaaaccgtgg cacggtctgg aatttcttct ggaggcgttc tgccgcctgc
       61 cggccagaga aaagctcaaa ctggtcatta tcggtgaagg cccgctgcgg caccagcttg
      121 aaaaagaggc cggcaagctg ggcttgcagg accgggtgat gttcacgggc gcggtgcgct
      181 ttgaacagat tccccgaacg ttaaagagtc tggacgcgtt cgtcgctccc tatccgcaga
      241 tcgacggctt ctatttttcg ccgctcaaaa tttttgagta catggccgcc ggtcgtccga
      301 tcgtggcgtc ccgcatcggc caggtggccg aaattctgca cgatggcaag aacgctctgc
      361 tcgttccgcc cgaagacccg gcggctctgg tctcggctct tcgccgtctg aaggccgacc
      421 ggaaattggg agcgcgtctg gggcgcgaag cgcagcgggc cgccttaaga gagcacacct
      481 ggcaggtacg gctggccacc ctcgaaccag ttttccattc cctggcggcc aaaagatagg
      541 tcggttgatg gacctgaaat tccgcatacc tctggcgaaa gtagattggg tggggctcgg
      601 ccgcattttc cggcacttcg gaaaacattt ggggccccat aagaaaagcc tcttctgggg
      661 cggcgcttcc cttctggggc tgatgctggt ggagctggcg cgcccctggt ctttgaaaat
      721 tgtcattgat tacatcctga ttccctccaa agccaaacgc gatttcggtt ttctggcacc
      781 cctctcccac tgggacccca tggccgttct cacatttgcc gccgggagcg tggcggtcct
      841 ctcactcggc ggggccctct tctcctacgc tcagaatgtc ctgttcgcct cggtcgggga
      901 aaaagtttca ggagccattc gtctggagct gttctcccac atccagcgcc tcccccagag
      961 ctaccacgac taccgggaga cgggtgaagt gctgatgcgc ctgaccggcg atattgattt
     1021 gctcaaagag cttttggtct cgctcttcat ctccttgggc agccggatct tcatcgtcgt
     1081 cggcatgctc gccctcatgc tctacatgaa ctgggaattg accctgatcg cgatcatcgt
     1141 gctgccgctc ctgttcctca ccagcctccg gttttccgta aaactgaaag aggcctcccg
     1201 gcgccagcgc aaaaaagaag gccagctcgc cgccgctgtc tacgagggag tttccgggat
     1261 cagcctgacc aaaattttcg gtcaggaaaa acgccacgac aagcttttgg gcaaatcagt
     1321 ctcctccgac gtcaaagccg gcctgcggac caccaagctg gagtctgcct atgcccgctg
     1381 ggtagacgtg atcaccgcgc tcggagtgat gttagtcctc ttcttcgggg tcaagcaggt
     1441 ccaggccggc cacctctcgg ccggcaatct gctcatgttc ctctcctatt tgcggagcgc
     1501 gtacaagccg ctgcgggatg tagcccagct ctcgtcccgg accgccaaag ccacggtgtg
     1561 cggcgaacgg gttctggaaa ttctggccat gcagccggaa gtgctggaca agccggaggc
     1621 ggttcccgcg caggacattc aaggggaaat ccgttttgcc aacgtcggtt tttcctacca
     1681 aaccggccgg ccggtgctct ccgatctctc tttccgcctc cccgccggtc aaacaaccgc
     1741 catcatcggc cccagcggtg ccgggaaatc gaccatcgcc aaactgctct tacgcctcta
     1801 cgagccggag gccggaacga ttttcgttga cggcgtgccg attggcgact accagattaa
     1861 atcgtttcgc aagcggatta cctcgctctc ccaggaggcc ttcctgttcc gcaccacgat
     1921 ccgcgacaac atctccttcg gcaaacccaa ggcgagtttc gacgaaattg tcgccgccgc
     1981 ccgcaaggtg ggggccgatg agttcatccg ggcgttgccg caggggtacg acaccctggt
     2041 cggggaaggc gggcagaccc tt
//
LOCUS       DBNGZA010000050         5626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1705456, whole genome shotgun sequence.
ACCESSION   DBNGZA010000050 DBNGZA010000000
VERSION     DBNGZA010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1705456"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16FU_01040"
     CDS             <1..190
                     /locus_tag="AC16FU_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992996.1"
                     /translation="DRCAAEAADLIFARGDFLQYCRERNIAAIPFASFFEIAENLKTI
                     LRGETPVTPLRAKKAAVL"
     gene            187..2697
                     /locus_tag="AC16FU_01045"
     CDS             187..2697
                     /locus_tag="AC16FU_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2992997.1"
                     /translation="MTHQMKAVIMAGGFGTRLRPLTATLPKPMVPVGGVPLMEHVVRL
                     LARHGFSDIIVLLYFQSQAIRDYFDDGKAFGVRLEYIQPTEDFGTAGSVALAAAKLSE
                     QFLVISGDVCTDIDLAQATAFHRTKQAQATMILTRRENPLPYGVVVTDPDGRITQFLE
                     KPTWGQVFSDTVNTGIYILQHDVLSAIPAQLRFDFSQNLFPLLLERKSALFGYVALGY
                     WRDIGNLEEYFQIHRDLLAEKVQLEIPLERKRTPSATLWVGSNVQIDPPAQFSGRVII
                     HRGARIDAQAAVANTVIGAGTVVGPEAAVKDSILWERVTVGERASLTGCVVASDAVIG
                     PEARVGEQVIVSEGTTIGRRAKILPGVKIWPGKKVEDEAVLGTSLIWGESWARELFSG
                     AKVAGLANRELTPEFVVKLGAAYGAFLGEGATVVTSRDASKVSRMLNRAVICGLVSAG
                     VHAQDLRTLPIPVVRYELKNGSQKGGLHVRHSPRDPATVEIVFFDAGGQDLPPGKTKA
                     MERLFFREDFRRAEPASVGQLDFPVRVIEHYREGFLQNIPAETIRQARFKVVVDYSWG
                     GAAEVLPGILGHLGLDTVSLDAYVDPQKLFQAEVVEKTGWTQLATIVRSVGADLGLWL
                     DPGGERLALVDEAGAVISGQRLLALFTFLVLQGLPVKELAVPVMATMAVEKLAKQYGA
                     SVSRVRNDHLGMMEAAGRSGVNFVGGTRGGFIFPAFQPGVDAMFAAAKFLELLARSQK
                     KISTLVAELPAYPMLTEEVPCPWEKKGQVMRRLLEETAHVERELVDGVRIRNADGWIL
                     VWPDRRSGTFTLTAEGRDPEQVKREVAEYRRLIELWQK"
     gene            2824..3267
                     /locus_tag="AC16FU_01050"
     CDS             2824..3267
                     /locus_tag="AC16FU_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2992998.1"
                     /translation="MKWKNNKPIKILLFIAIPIVLLPVGFFLLYLLFGGDWGIDLPGG
                     YFLSSIYGGAVEITDSPKNIVVYPNIETYKVYEQKHLVVGRVSNEGLQPREVENSVPG
                     YFILNTENGLLLQGLEEKVWLDSLRRYGITQKPRLHKPTGFDRNY"
     gene            complement(3480..4208)
                     /locus_tag="AC16FU_01055"
     CDS             complement(3480..4208)
                     /locus_tag="AC16FU_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HIR2992999.1"
                     /translation="MFCLAGAKGTLAQTPPESTHAPAAAFKSGTLSLSEKSWDFGYIP
                     KGSRVTHTFVIKNNGTDSLKNLKASPSCGCTAAPLDKTILAPGDSTYLEVSFNAKNYS
                     GPVTKTVYLQSNDPQNPYFDLLFTANVGTAIPNLSVTPPEVTFDTLREIPNSPTKLEI
                     KNINTDNKIGLEVIYAPKPFVDYKLTKKALKPGQAGEVSVRLKSDKMKPGEFFTSLTI
                     LCNDPNKSRFTVPIHGVYAANATK"
     gene            complement(4348..5367)
                     /locus_tag="AC16FU_01060"
     CDS             complement(4348..5367)
                     /locus_tag="AC16FU_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010597145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR2993000.1"
                     /translation="MRFSHSILDLIGHTPLVKLSKMAPPPGPTILAKLEYTNPGGSVK
                     DRMVWHIVREAEKAGLLKLGDTIVDNTSGNTGIGLALVAAVRGYHAILAVPDRVSQEK
                     IDLLTALGAEVIVTPSDVPIEGPRSYYQTAQRLARERGAFHLNQYHNPKNPEAHYLTT
                     GPEIWEDTAGKIDVLVAGIGTGGTITGIARFLKEQKPQIKVVGVDPVGSIHAEFIRTG
                     RPAAPEPYLLEGIGSEMVTQALDPEVVDTVLQVTDREAFLMVRKLAQKEGLLVGGSSG
                     AAFWAALEVARGMDSQSVIVTIFPDSGTRYLSKYFNDEWMRARGFLAPLRGHRSRVPS
                     AVSSP"
     gene            5366..>5626
                     /locus_tag="AC16FU_01065"
     CDS             5366..>5626
                     /locus_tag="AC16FU_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993001.1"
                     /translation="MDEARKINSGNVSMIFRFGLAVISSFSFFGSQPAQLKIGVSPAN
                     EGEKNSNWFAFSTVKVNLHNGCRESQRAGARGGGQSLYHENSG"
BASE COUNT         1166 a         1516 c         1709 g         1235 t
ORIGIN      
        1 cgaccggtgt gcggcggagg cggcggatct cattttcgct cgaggggact ttttgcaata
       61 ctgtcgggag cgcaatattg ccgcaattcc tttcgcatca ttttttgaga tcgcggaaaa
      121 cctcaaaacc attctccggg gtgaaacgcc ggtcacaccg ctccgcgcaa agaaagcagc
      181 ggtgctatga cgcaccaaat gaaggcggta attatggccg gcggctttgg cacgcggctc
      241 cggcccttga ccgccaccct tcccaaaccg atggtgccgg tgggcggcgt cccgctcatg
      301 gagcatgtgg tgcgcctttt ggcccgccac ggtttttccg acatcatcgt gctcctctat
      361 tttcaatcgc aggccatccg ggactatttc gacgacggca aagcgttcgg cgtccggctg
      421 gagtacatcc agccgaccga ggatttcggc acggcgggga gcgtcgcttt agccgcggca
      481 aaactctccg aacaatttct ggtcatctcc ggcgacgtct gtaccgacat cgatctcgcc
      541 caggcgaccg cttttcaccg gacgaagcag gcccaggcga ccatgatttt aacccggcgg
      601 gaaaatccat tgccgtatgg cgtcgtggtg accgacccgg acggtcggat cacgcagttt
      661 ttagaaaagc cgacctgggg acaggtcttc agcgacacgg tcaacaccgg catctatatc
      721 ctccagcacg acgttttgtc cgcaattccc gcccagctgc gctttgattt cagtcagaac
      781 ctgttcccgc tcttgctgga gagaaaatca gcacttttcg gttacgtcgc tctggggtac
      841 tggcgcgaca tcggtaattt agaggaatat ttccaaatcc accgcgatct attggctgaa
      901 aaagtccaac tggaaatccc cctggagcga aagaggacac catccgcgac actctgggtc
      961 ggtagcaacg tccagatcga cccaccggct caattctccg gccgggtcat tattcatcgg
     1021 ggtgcgcgga tcgacgctca agccgccgtg gccaataccg ttatcggggc ggggacggtc
     1081 gtggggccgg aggcggcagt caaagacagc atcctctggg agcgggtcac ggtgggggag
     1141 cgggcttccc tcaccggctg cgtagtggca agcgacgccg tgatcgggcc ggaggcgcgg
     1201 gtcggcgagc aggtcatcgt gagtgagggc accaccatcg gccggcgggc caaaattctt
     1261 ccgggcgtca aaatctggcc cgggaaaaaa gtggaagatg aagccgtact cggcaccagt
     1321 ttaatctggg gtgaaagctg ggcgcgggag cttttcagcg gtgccaaagt ggcggggctg
     1381 gctaatcgag agttgacgcc ggaatttgtg gtcaagctgg gtgccgccta cggcgcattt
     1441 ttgggagaag gtgccaccgt cgtcaccagc cgggatgcca gtaaggtgtc gcggatgctc
     1501 aaccgggccg tcatctgtgg gttagtctcc gccggggtgc acgcccagga tttacgcacc
     1561 ttgccgatcc cggtggtgcg ctatgaacta aagaacggat ctcaaaaagg cggcctccac
     1621 gtgcggcatt ccccgcgcga tcccgccacg gtggaaatcg tcttcttcga cgccggcgga
     1681 caggacctgc cgcccgggaa aaccaaggcg atggaacggc tcttcttccg ggaagatttt
     1741 cgccgcgccg aacccgcgtc ggtgggacag ctcgactttc cggtgcgggt gattgaacac
     1801 taccgggagg gtttcctgca aaacattcct gccgaaacca tccggcaggc acggttcaaa
     1861 gtggtggtgg attactcctg gggcggggcg gccgaagtgc tccccggcat cctcggccac
     1921 ctcgggctgg ataccgtctc tttggatgcg tacgtcgatc cgcagaagct cttccaggcg
     1981 gaggtcgtgg aaaaaaccgg ctggacccag ctcgctacca tcgtccgctc tgtaggagcc
     2041 gacttgggtc tctggcttga tcccggtggg gaacggctgg cgctggtcga tgaagccggt
     2101 gcggtcattt ccggccaaag gttgctggca cttttcactt ttctcgtttt gcagggtttg
     2161 ccggtcaaag aactggcggt accggtgatg gcgacgatgg cggtggagaa attagccaag
     2221 cagtacggcg cgtctgtttc ccgcgtccgc aacgaccact taggcatgat ggaggccgcc
     2281 ggtcgctccg gggtcaattt tgtcgggggg acccgcgggg gattcatctt cccggccttc
     2341 cagccgggag tcgatgcaat gttcgccgcg gccaagtttt tggagcttct ggcgcgttcg
     2401 caaaagaaaa tttcgacgct ggtagccgag ctccccgcct acccgatgct gaccgaagag
     2461 gtgccctgcc cctgggaaaa aaagggccag gtgatgcggc ggctgctcga agaaaccgct
     2521 cacgtcgagc gtgaactggt ggacggggta cgcatccgca acgcagatgg ctggattctg
     2581 gtctggcccg accggcgaag cggcaccttc accctcacgg cagaaggacg cgaccccgaa
     2641 caggtcaagc gggaggttgc agaataccgg cggttaatag agttgtggca aaaataaagg
     2701 caactatcgc tttagggcag aactcatttt ttctcaactt gtatggggat taaagtttag
     2761 gtccttctca cccgattaac gcatcgtggc gtgcaagtgt tgccggcatc gacggatttt
     2821 ataatgaaat ggaaaaacaa taagccgata aaaattttac tcttcatagc catacctata
     2881 gtattgttac ccgtcggatt ctttcttttg tatttactat tcggcgggga ctggggtatc
     2941 gatctccccg gtggctattt ccttagcagt atatatggag gagcggtaga aatcactgat
     3001 tccccgaaaa atattgtcgt ttaccccaat attgaaacat ataaagttta tgagcaaaaa
     3061 caccttgtgg tggggcgtgt gtcaaacgaa ggcctgcaac cgcgtgaagt tgagaacagt
     3121 gtgcccggct atttcatact taatactgaa aatggcctac tattacaagg gcttgaagag
     3181 aaagtttggc tggattccct tagaagatac ggaataacgc aaaagccgcg cttacacaaa
     3241 ccgaccggat ttgatcgaaa ttattgatga ttccgtgttg tgttatttgt ttgagaacag
     3301 ccccttttct ttggcttctc acttcccaaa aaataaatga gggtgttgcg ccggtggggt
     3361 agagggggct tggaaactac cccatgaacg gaggtccacc ctctgaattc tccagtataa
     3421 caaatgcccc ctgaccggtt ctgtcaaggg gcaggtcgtc tcgcttcgga acgcaatgat
     3481 cacttggtgg cgttggcggc gtaaacaccg tggatcggca cggtgaagcg cgacttgttg
     3541 gggtcgttgc agagaatcgt gagcgaggtg aagaattcac ccggtttcat tttgtccgac
     3601 ttcagccgca ccgaaacctc ccccgcctgg ccgggtttga gcgccttttt ggtcagtttg
     3661 taatcgacga acggcttggg cgcgtaaatc acctcgaggc cgattttgtt gtcggtgttg
     3721 atgtttttaa tctcgagctt cgtgggggaa ttcggaattt cgcgcagcgt gtcgaacgtc
     3781 acttccggcg gagtcaccga gaggttgggg atggcggtgc ccacgttggc agtgaagagc
     3841 aaatcgaagt acgggttctg cggatcattg gactgcaggt agacggtctt ggtcaccggg
     3901 ccggagtagt ttttggcgtt gaaggagact tccaggtagg tcgaatcgcc cggagccaag
     3961 atcgtcttat ccaaaggcgc cgcggtgcag ccgcaggagg gggaagcctt caggttcttc
     4021 agcgagtcgg tcccgttatt cttaatgacg aaggtatggg tgacgcggga acctttgggg
     4081 atgtagccga aatcccacga cttttcggac agggagagtg tgcccgattt gaaggcggct
     4141 gccggagcat gggtgctctc cggaggggtc tgagccagtg taccttttgc gcccgccaga
     4201 caaaacatca gtgcgaaaaa cgctgcgaga aaaaattgct tccgtttgtc ctgcatacct
     4261 caccgctcct gttctttggg ttcctctctt ctccgtcagg cgtatcgata gggaaacgtg
     4321 tccaccggac gagacgttgg ctttttttta gggggaagaa acggcggagg ggacccggga
     4381 tctgtgaccc cgcaaaggcg ccaaaaagcc gcgggcgcgc atccactcgt cgttgaagta
     4441 cttgctcaag tagcgcgtgc ccgaatcggg gaagatggtt acgatgacgc tttgggagtc
     4501 cattccccgg gccacttcaa gtgcggccca aaaggcggcg ccggaggagc cgccgacgag
     4561 cagaccctcc ttttgagcca atttccggac cataaggaag gcctcgcggt cggtcacctg
     4621 gagcacggta tcgacgacct ccggatccaa ggcctgcgtc accatttctg agccaatgcc
     4681 ctccaggaga tacggttccg gcgccgccgg tctgccggtg cggatgaact cggcatgaat
     4741 ggaaccgacc gggtcgactc cgaccacttt gatctgcggc ttctgctcct tgagaaaacg
     4801 ggcaattccg gtgattgtgc cgccggtgcc gatgccggcc accagcacgt caattttccc
     4861 cgccgtatcc tcccagattt caggacccgt ggtcaggtag tgggcttccg gattcttcgg
     4921 gttgtgatac tggttcaggt gaaaagcacc ccgttcccgg gcaaggcgct gggcggtctg
     4981 gtagtatgac cggggaccct cgatcgggac atccgagggg gtgacgatca cctcggcacc
     5041 cagagcggtg aggaggtcga ttttttcctg gctcacccgg tcggggactg ccagaatcgc
     5101 gtggtagccc cgtacggcgg ccaccagcgc cagcccaata ccggtattcc ccgaggtgtt
     5161 atccactatg gtgtccccaa gttttagcaa tccagctttc tccgcctccc gcacgatgtg
     5221 ccagaccatc cggtctttga ccgaaccgcc ggggttggtg tactccagct tggccaagat
     5281 ggtggggccg gggggcggtg ccattttgga aagtttgacc aagggtgtgt ggccgatgag
     5341 gtcgagaatc gaatgggaaa agcgcatgga tgaggcaaga aaaatcaatt cggggaacgt
     5401 gtcaatgatt ttccgctttg ggctggcagt catctcctcc ttttctttct tcggcagcca
     5461 gcccgcacag ctcaagatcg gcgtttcacc ggctaacgag ggagaaaaga attcaaactg
     5521 gtttgcgttt tcaactgtga aggttaattt acataacgga tgccgagagt cgcaaagagc
     5581 cggtgccaga ggcggaggtc aatctcttta ccatgaaaat tctggt
//
LOCUS       DBNGZA010000051         5799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2448313, whole genome shotgun sequence.
ACCESSION   DBNGZA010000051 DBNGZA010000000
VERSION     DBNGZA010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2448313"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="AC16FU_01070"
     CDS             complement(<1..1049)
                     /locus_tag="AC16FU_01070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2993002.1"
                     /translation="MFSILFLVAFGLRFLFPAADPPWHLSWSQDLSGDPPQYTSGARN
                     HVLFGQWEPLGDLRFVTFSKSALTLFAYPVFLLFGVGRWQATLVAAFMSTLIVVLLAL
                     GLRRVFGWRAGFCAGSLLAVSTFATFYGRVTFLEIPLLLFLAGVFFCFCLSRDSLFIS
                     GCAGACCALSVFFGKPFGFVGVPALAGGWFNRHWSSPRKTARMFVRPLWGITTGFLVI
                     GLFWFFWVFLPNPNEVRSYYREQSLGLYGAPLGLESLSGFFLQVASLGNDTAYFSRQP
                     VLSLLGSAALVLLGWRLRSPRRPRSPAEKNQLSIYIFLAVWLIVGYLGLMPWNYRPLR
                     YQLLLTFPLCGISGIL"
     gene            complement(1230..1919)
                     /locus_tag="AC16FU_01075"
     CDS             complement(1230..1919)
                     /locus_tag="AC16FU_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993003.1"
                     /translation="MSKQPLLTVVIPVYNEERTLSKVLERVFASPLDLQVIVVNDGST
                     DGTEEVLAHSPFPILHLKNPVNNGKGASIRKALEHARGEYVIVQDADLEYDPQDFQKL
                     LACAQRYQTPVVFGRRIRTWKEKIRHWKFFTAGKLLTWLANILYGCRVTDEPTCYKLV
                     RTDLLKALDLQAQRFEFCPEVTAKVAKRGIRIGEVPISYSPRDKKGGKKIKYRDFFEA
                     VWTLLRYRFRS"
     gene            complement(2004..2132)
                     /locus_tag="AC16FU_01080"
     CDS             complement(2004..2132)
                     /locus_tag="AC16FU_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993004.1"
                     /translation="MAESVLQLIRIPKVQIAAVAQELDLPHRKEMAVRDGTGQRSP"
     gene            complement(2125..2373)
                     /locus_tag="AC16FU_01085"
     CDS             complement(2125..2373)
                     /locus_tag="AC16FU_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993005.1"
                     /translation="MRSRLCHRAHDRQERVFSHRGRGKADRWFDRTAAFSYSPSEDRL
                     RMLLFFANDVVSGIGVLTLRWEIAGPATKKPGGGYLRG"
     gene            complement(2657..3982)
                     /gene="pilQ"
                     /locus_tag="AC16FU_01090"
     CDS             complement(2657..3982)
                     /gene="pilQ"
                     /locus_tag="AC16FU_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR2993006.1"
                     /translation="MRQSKRLVVKLLPVVACALLVWAAAGRGQGDQDKIIKSLNFQNA
                     EMRSVFGMLADYAKQNIVIAPTVTGTVTLALSDITWRQAFDIILKTYNLAGVQEEGYI
                     RVLPLRDYLSEQTEIEKARAEQKALVAVQLRVIPVENASAKDMKAAVKAMLSDRGFVD
                     TDERTNSIIVRDIPDNIVKIEEFVKGLDKETKQVKISAQLVEIKSEFLQELGVRWFAR
                     DSFFVDAFRTNNIQGHYEFEQRADAKVTDPVDNFTVGAIDRAFNLEAAIRAFAKDNKA
                     KVLAHPEVTTLDNKMAKIQIGSKIPIKQFDASGNVVISFINVGTLLEVTPHVTSEKRI
                     LLALKPEQSTFRTEAIGPVIDTKNAETNVVVNNGQTVVIGGLTTSTEEKQTVGLPFLK
                     DIPVLGYLFRYTKKRLDTSDLVIFVTPTMVTSETMAPETGMTEPVPSGN"
     gene            complement(4002..5555)
                     /locus_tag="AC16FU_01095"
     CDS             complement(4002..5555)
                     /locus_tag="AC16FU_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993007.1"
                     /translation="MRLSRMVPGVFAVVCSAAVALAGDVSITEISIHRVGESVDVAVH
                     GTGPLTVTPKMAGKSGTQPFRLILDFPRAVRAFKGQSFNSLPTATLSAIRTSQYAVTP
                     EKVVRVVLDFAARPNYQIEQRGSAIVIHLPATPDEPNFPVWSTATVGTAPAPVPTLAA
                     TPEPEKAVPPLTEPAATPLEPDTAALEQGETQPETSAAEGKENDTSAVASEEMAPADS
                     QPLEPDATTAATPETTAGQAAAAEPTLSPGETLTSMAPAPADTSAESQPVRDKLFASA
                     ESLPSPPAPGANHLTASASNSTAVAPQQPAHLLPSAASTPETTGKAVSQAEPPAPKKS
                     SPAPPVKTPAGSAPAAEPTPPEVSVTDLTEPQGPTPAEAEEEVTEEEINPDEVAVEED
                     STEENFAPIPGVMLQTSPTRTLVKYHLYGRDPFSPLVEKAVGLSGKHRGRLPDVEALR
                     LVGILRGWSGSRALFEDREGRGYILRDGDRVRNGHVVSVSERKVVFQINEYGWTRTVA
                     LTLLSDENE"
     gene            complement(5568..>5799)
                     /gene="pilO"
                     /locus_tag="AC16FU_01100"
     CDS             complement(5568..>5799)
                     /gene="pilO"
                     /locus_tag="AC16FU_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIR2993008.1"
                     /translation="AFTLSLRGSYHAIGNFLAAVANFPFIVNVSQLALSQPAVTQQAA
                     AAKNPNETVSASFRILTYYVREEEKLKKLELN"
BASE COUNT         1170 a         1729 c         1748 g         1152 t
ORIGIN      
        1 agaataccgc ttataccgca caggggaaat gtcagcagca actggtagcg cagcgggcgg
       61 tagttccagg gcatgagtcc caggtacccg acgatcagcc agaccgcgag gaaaatatag
      121 attgacagct gatttttttc ggcaggcgac cgcggccttc tgggtgatcg cagtcgccaa
      181 cccaagagta cgagggcggc ggagcctaag agggaaagaa ccggctgccg ggagaaatag
      241 gcggtgtcat tccccaacga agcgacctgc aggaaaaagc cggataggga ctcgagtccc
      301 aggggtgcac catacaaccc cagggactgc tcgcggtagt aggagcgaac ctcgttcggg
      361 ttgggcagga aaacccagaa aaaccagaag agtccgataa ccagaaagcc ggtagtgatt
      421 ccccaaagtg ggcgaacgaa catccgagcg gtcttgcgag gactagacca gtgtcgattg
      481 aaccatccgc cggcaagggc gggcacgccg acaaacccga acggtttccc aaagaaaaca
      541 ctcagcgcac agcatgctcc agcgcagcca ctgatgaaaa gcgaatcccg gctgaggcag
      601 aagcagaaga agacacctgc caggaagagt aagagaggaa tttcaagaaa cgtcacccgt
      661 ccgtagaaag tagcaaatgt gcttaccgcc aaaagtgaac cggcacagaa tcccgctctc
      721 caaccgaaga cccgacgcaa ccccagtgct aacaggacga caatcagcgt actcatgaaa
      781 gctgcaacca aagtcgcctg ccaccgccca acaccaaaga gcaaaaagac aggataggcg
      841 aaaagcgtca acgccgattt cgaaaaggtg acaaaacgaa ggtccccgag cggctcccac
      901 tgtccgaaga ggacatgatt gcgcgcgcca ctggtatact gcggcgggtc gccggagaga
      961 tcctggctcc aggagagatg ccaaggcgga tccgctgcgg gaaacaggaa gcgcaacccg
     1021 aaggcaacca aaaacagaat actaaacaaa aagaggtcac gccgccacgc ttttaccccg
     1081 ctttggccca gagagagcgc ggatatcagg gccggctctt ttctgtctgc acccatcccg
     1141 cagcagtata cgatgctcgt gattcgcgcg ccaaccgaga gtgtcgtggc acgtgacaga
     1201 agagcagaat cgctgcggac gaaatgcgct caggaccgga aacggtagcg gagcaaagtc
     1261 cacaccgctt cgaagaaatc ccggtatttg attttcttgc cccctttttt atcgcggggg
     1321 gagtaggaaa tcggcacttc gccgatccgg attcctcgtt tggccacctt ggcggtcact
     1381 tcgggacaga actcgaaccg ttgcgcctgc aggtcgagcg ccttgaggag gtcggtgcgg
     1441 accagtttgt agcaggtcgg ttcatcggtg acgcggcagc cgtagaggat attcgcgagc
     1501 caggtaagga gtttgccggc agtaaagaat ttccaatggc gtattttttc cttccaggtg
     1561 cggatgcgac gaccaaacac caccggcgtc tggtacctct gggcacaggc caggagtttt
     1621 tggaaatctt gaggatcata ttctaaatcc gcatcctgca cgatcacata ctccccgcgg
     1681 gcgtgttcca gcgctttccg aatcgacgcc cctttgccat tgtttaccgg attcttcaaa
     1741 tggagaatcg ggaatggcga gtgagcgagc acctcctccg tgccgtcggt ggagccgtcg
     1801 ttgacgacta tcacctgtag gtcgagcggc gatgcaaaga ctctctctaa tactttgctc
     1861 agagtccgct cttcattgta gaccggaatc acaacggtca gaagcggttg tttgctcatc
     1921 agtttctcag ggggagaatt tcctccgggg acggccccaa aaggctcacg tgccccccgc
     1981 tcggagcggc ctcgcgggat gattcagggt gatcgctgac cagttccgtc ccgcaccgcc
     2041 atttctttgc ggtggggcaa atcgagctcc tgcgccaccg cagcgatctg caccttgggt
     2101 attctgataa gctggagaac ggattcagcc acggaggtaa ccacctcccg gcttcttcgt
     2161 cgccggtccc gcaatttccc accgcagggt cagcactcct atcccgctca ccacatcgtt
     2221 cgcgaagaaa agcaacatcc gcaggcgatc ctcagaagga gaataagaga aagcagccgt
     2281 tctgtcaaac cagcggtcgg ctttcccccg tccccggtgc gagaagaccc gctcctgacg
     2341 gtcgtgagct cggtggcaca agcgggatcg cacccggtgc gctcgtggtt gaatttcctc
     2401 cctccgccga cgaaaggaat cgccagagcg tggggcgcca aagggggcta ccatcgcgtt
     2461 cgtgaccttt tcctctttcc cctcagcctg ctgccgcaac catcgacgga ggtgttcctg
     2521 atccggtgct gttttccctc tggtggtgaa agacgaaacc ccataccaag cgagtgggaa
     2581 gcgcaggaaa gagaagtgct tccagaaaag aaaagggctc ccggtgggga gccccagtcg
     2641 gccattctct cgccggctaa ttgccgctcg gtaccggctc cgtcatgccg gtttcgggcg
     2701 ccatcgtctc gctcgtcacc atggtggggg ttacgaagat caccaagtcg ctcgtgtcca
     2761 ggcgcttctt ggtgtagcgg aacaggtacc ccaggaccgg gatgtccttc agaaagggga
     2821 gccccaccgt ctgcttctct tcagtggaag tcgtcagacc gccgatcacc accgtctgtc
     2881 cgttgttcac cacgacattg gtctcggcgt tcttggtgtc gatcaccgga ccgatggcct
     2941 cggtgcggaa ggtgctctgc tccggcttga gcgccagtaa aatgcgcttc tcggaggtga
     3001 cgtggggggt cacttccagg agggtgccca cgttgatgaa gctgatcacc acgttgccgg
     3061 aggcgtcgaa ctgcttgatc ggaatcttgg aaccgatctg aatcttggcc atcttgttgt
     3121 ctaaggtcgt cacctccggg tgcgccagga ctttcgcctt gttgtctttg gcaaaagccc
     3181 ggatcgccgc ctcgaggttg aacgcccggt cgatggcgcc tacggtgaag ttgtccacgg
     3241 ggtcggtcac cttcgcgtcg gcccgctgct caaactcgta gtggccctgg atgttgttgg
     3301 tccggaacgc gtcgacgaag aacgagtcgc gcgcaaacca gcggaccccc agttcctgga
     3361 gaaattcgct tttaatttcc accagctggg ccgagatctt gacctgcttg gtctctttgt
     3421 cgaggccttt tacgaactct tcgattttga cgatgttgtc cggtatgtcg cgcacgatga
     3481 tggaattggt gcgctcgtcg gtgtccacga agccccgatc ggagagcatg gccttgaccg
     3541 ccgctttcat gtccttggcc gaggcgttct ccaccgggat cacccgcagc tgcaccgcca
     3601 ccagggcttt ctgttctgcc cgagcttttt cgatttcggt ctgttcagac aggtagtcgc
     3661 gcaaaggcaa aacccggatg tacccctctt cctggacccc ggctaagtta taagtcttca
     3721 gaatgatgtc gaaggcctgg cgccaggtga tgtcggaaag ggccagggtc accgtgccgg
     3781 tcaccgtggg ggcgatgacg atgttctgct tggcatagtc ggccagcatg ccgaagaccg
     3841 accgcatttc cgcattctgg aagttgagcg atttgatgat tttatcctgg tcgccctggc
     3901 cccggccggc cgccgcccag accaaaaggg cgcaggccac caccgggagc agtttgacca
     3961 ccagccgttt cgattgccgc atagcgccac ctcgcctttc gtcactcatt ttcgtcactc
     4021 aaaagcgtca acgcgacggt ccgcgtccag ccatattcgt ttatctgaaa gaccactttc
     4081 cgttcactca ccgagacgac gtggccgttt ctcacgcggt cgccgtcgcg cagaatgtag
     4141 ccgcgccctt cccggtcctc aaacaaggcc cggctgcccg accagccccg gagaatcccc
     4201 accaggcgca gcgcttcgac atcgggcaga cggccgcggt gcttgccgct gagccccact
     4261 gctttttcca ccagcgggct gaagggatcc cggccgtaga ggtgatactt caccaaggtc
     4321 cgggtcgggc tggtttgcag catgacgccg ggtatcggcg caaaattctc ttccgtcgag
     4381 tcttcttcga ccgcgacctc atcgggattt atctcttctt cagtcacttc ctcttccgcc
     4441 tcggcgggag tcggtccctg cggctcggtc aggtcagtga ccgaaacttc cggcggcgtc
     4501 ggctccgcag ccggggctga cccggccgga gtctttacgg gcggagcggg ggatgacttt
     4561 ttgggtgccg gcggctccgc ctggctcacc gctttccccg tcgtttcggg tgtactggca
     4621 gccgacggca agaggtgcgc gggctgctgg ggtgccaccg cggtcgaatt cgatgccgag
     4681 gccgtcaggt gattcgcgcc tggcgccggt ggagacggta gcgactcggc gctggcgaaa
     4741 agtttgtccc gtacgggctg actctccgcg gaagtatctg cgggagccgg ggccatgctc
     4801 gtgagggtct ctccgggtga aagagttggt tcagccgccg ctgcctgacc ggcggtcgtt
     4861 tccggtgtcg ccgccgtagt ggcgtccggc tctaaaggtt gactatccgc cggtgccatt
     4921 tcctcacttg caactgcgga ggtgtcgttt tcctttccct ctgcagccga ggtttcgggc
     4981 tgcgtttcac cctgttccag cgctgcggtg tccggctcca gtggtgtggc tgccggttcg
     5041 gtcaaaggcg gtactgcctt ttccggttca ggcgtcgcgg ccaaagtagg tacgggtgcg
     5101 ggagccgtcc cgacggtcgc ggtggaccat accgggaaat tcggctcgtc gggggtggcc
     5161 ggcaggtgaa tgacgatggc agagccgcgc tgctcaatct ggtaattggg ccgggccgca
     5221 aaatccagaa caacgcggac gaccttctcc ggggtcaccg cgtactggct cgtgcggatg
     5281 gccgaaagcg tggcggtggg caaggagttg aaactctggc ctttgaacgc ccggaccgcc
     5341 cgcggaaagt ctaagatgag ccggaacggc tgcgtgccgc tcttgcccgc cattttcggg
     5401 gtcacggtga gcgggccggt gccgtgaacg gccacgtcca ccgactcccc cacccggtgt
     5461 atggaaattt cggttatcga gacgtcaccg gccaacgcta ccgccgcgga acaaacgacc
     5521 gcgaacactc cgggaaccat tcgggaaaga cgcatcgccg tcacctatca gttaagttcc
     5581 aatttcttca atttctcttc ctcgcggacg tagtaggtca aaatccgaaa agaagcgctc
     5641 acggtctcat tggggttttt ggctgcggcg gcctgctggg tgaccgcggg ctgggacagc
     5701 gcgagctgcg acacgttgac gatgaacggg aaattggcca ccgcggccag gaagttgccg
     5761 atggcgtggt agctgccgcg caggctcaag gtgaacgcc
//
LOCUS       DBNGZA010000052         6000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2872518, whole genome shotgun sequence.
ACCESSION   DBNGZA010000052 DBNGZA010000000
VERSION     DBNGZA010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2872518"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="AC16FU_01105"
     CDS             complement(<1..1036)
                     /locus_tag="AC16FU_01105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2993009.1"
                     /translation="MERLFLVLLFILALGLRLLYLFEVAQSNPYFAHPTLDAGYHDDW
                     AQSIAGGNLIGSGVFFRAPLYPYLLGFVYAVFGHDYFAARAVQHTLGALAVILIFFLG
                     RRLFNRTVGALAALLAAFYPILIYFEDELLLDWLLVFLGTALFLLLYRAKEKPAAGRW
                     LVAGLMFGFACITRPTFLPFFVCVIGWIFYLFWPQKNWPGIARSAILFSLGTATIIAP
                     VSVRNWFVGHDFVLIASQGGTNFYIGNNPAADGHTSRLPEPFGVTWEYRDLQYPVDQY
                     LGRPAKPSEVSDYWYREGWRFIRENPRAALQLTLKKFYLLWNKFEIPNNQNFYTFSRN
                     VSLFRWLPLGF"
     gene            1212..3878
                     /locus_tag="AC16FU_01110"
     CDS             1212..3878
                     /locus_tag="AC16FU_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR2993010.1"
                     /translation="MGRLVVGLVFLSLVCSGGGFLAAEAAPPHPRFKERLLATGEYEA
                     FLGKLAQARQEGWDQPNPQPFRLSPLALAGLAVDTLKVVVLLVDFSDNQMTAGFANAN
                     PDTIQKILFSRGYIASGSMAEYYDEVSLGKLYVTGQVFGPFHMDSTYAYYVNNNGGLN
                     NTCPRCARQLTRDAIAKADTAVNFSQFDGNGDGRVDGLIIMHAGQGAEEGGISSRIYS
                     HFSNTGFPADSADGKSIPFYIAAPEEAGYAFPAPGLVTIGVPTHEFGHNLGLPDLYDV
                     DDVVNGRWRGLGDWSLMASGNYLGDGIYSGRKPGHLDPWSKIRLGFAAVDTVRTNRSA
                     VPIPAVETSEKIFRLWIFGGTGPQYFLVENRRKKLFDTDLPGSGLLIYHVDETVIDIN
                     LNPYNGNPSHPFIVVEQADGRRQMETDSSDGDAGDPYPGTTNHRTFDDLSNPNSRNYL
                     NQSTQVEVLNVSNSADTMAADLNVRWSRPLFQFSGTSVNDSINGNPAPNLRADSGETV
                     ELMITLKDLQASTDSVRLRLRSQDTLVSVSDSVGFIPAIAGSGGQASNTGDRFVVTID
                     PRTGRSRLVNFLLCAWSKDSSITLDTVFHLAVGPTDILIVDDDTGKTYETYYEAPFDS
                     LRIPYDVWHKKTQGRVDSLRLRQYPKVVWFTGDRRCTATCTRPLTVNDIIDLKAYLNT
                     GGKLFLTGQDIAQSLAGTADSTFLRDYLHCRHFSTGFPFAARGVLGDPVTGFIDSVYF
                     GAPPDGAGNQQSPDLLTPGDSASAVFKYTPSGQVAGTRFKGQWQTVFFAFGFEAISST
                     RSGYNTRMDVMNAVMNWFDTGAVCALLGDASGDRVVDIVDLVVLVNYVVFGSPIPTGI
                     NCTELTGDGVIDILDIICLLNYVVFGNPIPCG"
     gene            complement(4024..4950)
                     /locus_tag="AC16FU_01115"
     CDS             complement(4024..4950)
                     /locus_tag="AC16FU_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993011.1"
                     /translation="MKKNLIVLVIGEAFLVFTTSWLSSASTSTTTVSPQKYVRGDALT
                     NKSGSGIENRRLYRVDALTSSRVLIYEAEGDFISDILSSPNGKFIGFLEGKNQMRSLV
                     IIRPEGQVADRLKSNIHKFSWSPTSGKVAFIQGVTSEGRWVIPQGVAIYDLMTKSIKK
                     IADSALFLNWAIFDSNIYYSWNQRYYRYETKTGITEIVPYLGIDFSPDGKYYLSSHPD
                     ESFQVFERERNMNITDRFYKRFKHTPIRPTWYGGDSHLLLITYREVITDPKYENRPAK
                     PIIGIREYHYYLYDIEKDSTVKEWREKPLQKE"
     gene            complement(5174..>6000)
                     /locus_tag="AC16FU_01120"
     CDS             complement(5174..>6000)
                     /locus_tag="AC16FU_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2993012.1"
                     /translation="SARRDKPFVSVNCAALPETLLESELFGHRKGAYTGADRDKIGLF
                     AEADGGTFYLDEVSELPPAIQVKLLRVLEEKEVTRLGDTRPQKVDVRVISAGNKDLKT
                     QMEKELFRADLYYRLCTLTFKLPPLRERKEDLPLLVEHFVKKHSPAGATTLPRIDPAF
                     LRLLTEYDWPGNVRELENEIRKVLLLLGEGTRLTPDLLSGKFFGPDGDGLGKTTNGSF
                     SLYEHISQFERRYLLKALSENRWIKKRAASALQIPESTLRVKMKQYGLQEEKETET"
BASE COUNT         1346 a         1612 c         1652 g         1390 t
ORIGIN      
        1 agaaacccag aggcagccag cggaaaagcg aaacgttccg tgaaaaagtg tagaagttct
       61 ggttattggg aatttcaaac ttgttccaga ggaggtagaa tttcttcagc gtcaattgca
      121 gggcggctcg gggattttcc cggatgaagc gccagccctc ccggtaccag tagtccgaaa
      181 cttcagacgg ctttgccggg cggcccaaat actgatccac cggatactgc aaatcgcggt
      241 actcccaggt taccccgaac ggctctggca ggcgggaggt atggccgtcg gcagccgggt
      301 tgttgccgat gtagaagttc gttcccccct gcgaggcgat caaaacgaaa tcgtgaccca
      361 caaaccagtt ccgcacgctc accggcgcaa ttatcgtggc cgtccccaga gaaaacaaga
      421 tggcggagcg ggcaatgcct ggccagtttt tctgcggcca gaacagataa aaaatccagc
      481 cgatgacgca cacaaagaaa gggaggaatg tcggtcgggt tatacaagcg aagccgaaca
      541 tgaggccagc aaccagccag cgccctgccg caggtttttc tttcgcacga tagagaagga
      601 ggaacagggc ggtgccgaga aagaccaaaa gccagtcgag caggagttcg tcctcgaaat
      661 agattaagat ggggtaaaaa gccgccagaa gcgcggccag cgcccccacg gttcgattga
      721 acagccgccg ccccaaaaaa aagatcaaga tgacggccag ggcaccgagg gtatgttgaa
      781 cagcccgcgc cgcaaaatag tcgtgcccga ataccgcgta gacgaagccc aggaggtagg
      841 ggtagagggg cgcccggaag aagactccgc tgccgataag gttgccccct gctatactct
      901 gtgcccagtc gtcgtgatac ccggcgtcca gggtgggatg ggcgaagtag ggattacttt
      961 gggcaacttc gaaaagatag agaagccgca agcccagtgc cagaatgaac aataaaacga
     1021 ggaagagtcg ttccacttct ctggaaatag gccggcgtat cagaggcatc accgccaata
     1081 tatggccggg ttccccggcc tcaatggttc aaaattcgct ctccgggaat cacttttgcg
     1141 tattatatta tacctgtagc catccggtgt ccggcgcttt gtcatttctc cagtttgcgg
     1201 tggaggcggt aatgggtagg ttggttgtgg gtttggtttt tctttccctg gtctgttcgg
     1261 ggggtggttt tctggcggct gaggcggcgc cgccgcaccc gcgcttcaaa gagcgcctgc
     1321 tggccaccgg tgaatacgaa gccttccttg gcaagctggc acaggcgcgc caggaggggt
     1381 gggatcaacc caatccccag cctttccggt tgagccccct tgctctggcc gggttggcgg
     1441 tggataccct caaagttgtc gtcctcttag tcgatttcag cgacaaccag atgaccgcag
     1501 ggttcgccaa tgccaatccc gatacgatcc aaaaaattct tttctcccgc ggttacatcg
     1561 cctccggaag tatggcggag tactacgatg aagtttcttt gggcaaactc tacgttaccg
     1621 gtcaggtgtt cggtcctttt cacatggata gcacctacgc atactacgtc aataacaatg
     1681 gaggtttgaa taacacctgc ccccgatgcg cacggcagtt gacgcgggac gccatagcca
     1741 aagccgatac agcggtcaat ttctctcagt ttgatggtaa tggcgatggt cgagtggacg
     1801 gactaatcat tatgcacgcc ggacagggag cggaagaggg aggcatctcc agccgcatct
     1861 attcgcactt ttctaatacc ggatttccag cggattctgc agatggtaaa tcaattccct
     1921 tctacattgc cgcacccgaa gaggccgggt atgcctttcc ggctcccggg ctggtaacca
     1981 tcggcgtgcc gactcatgaa tttggccaca acttgggatt gcctgatctc tacgatgtcg
     2041 atgatgtggt caatggccgg tggcgggggt tgggcgactg gtcgctcatg gcttccggca
     2101 attacctggg agacggaatc tacagcggcc gcaagccggg ccacctcgat ccctggtcca
     2161 aaatccggct gggatttgcc gcagtggata cggtccggac gaatcggtcg gccgtgccta
     2221 ttcccgccgt cgagacctcc gaaaaaattt tccggctctg gattttcggc gggaccggac
     2281 cgcagtactt tttggtggaa aaccgccgca agaagctctt cgataccgac ctccccggct
     2341 ccggcctttt aatttaccac gtcgatgaga cggtgataga tattaacctt aacccctaca
     2401 acggcaaccc cagccaccca ttcattgtcg ttgagcaggc cgacggccgt cggcaaatgg
     2461 agaccgactc cagtgacggc gacgcgggcg atccgtatcc cggcaccacg aaccaccgca
     2521 cttttgacga cctctccaat cccaacagcc ggaattacct gaaccagtcg acccaggtcg
     2581 aagttttgaa cgtttccaac agcgccgaca cgatggccgc ggatctgaac gtcaggtggt
     2641 cgcggccttt gttccagttc tcgggcacga gcgtcaacga ctccataaac ggcaatcccg
     2701 cgcccaacct gagggccgat tcgggcgaga cggtcgaatt gatgataacg ctgaaagacc
     2761 tccaggcctc aaccgacagc gtccgcctgc gcctgcgttc gcaggatacg ctcgtctcgg
     2821 tgagtgacag cgtcggcttc atcccggcca tcgccggcag cggcggccag gccagcaata
     2881 cgggcgaccg attcgtcgtc accatcgacc cgcgcaccgg cagatcgcgg ctggttaatt
     2941 ttctcctttg cgcatggagc aaagacagtt ctatcacctt agacaccgtg tttcatttgg
     3001 cggtcgggcc gaccgacatc ttgatcgttg atgacgacac cggcaagacg tacgagacgt
     3061 actatgaagc tcccttcgac agtttgagga tcccgtacga cgtgtggcat aagaaaacgc
     3121 agggccgggt cgatagcttg cggctcaggc agtacccgaa ggttgtctgg ttcaccgggg
     3181 accgccgctg cacggctacc tgcacacgtc ctttgaccgt caacgacatc attgacctta
     3241 aggcctacct gaataccggc ggcaagctct tcctcaccgg gcaggatatc gcccagagtc
     3301 tcgccgggac tgccgattcg accttcctgc gtgactacct ccactgccgg cacttctcca
     3361 ccggcttccc ctttgcggcc cggggggtat taggagatcc ggtcaccggg ttcatcgact
     3421 cagtttattt cggcgctccg ccggacgggg ccgggaacca gcaaagtccc gatctgctca
     3481 ccccgggtga ttccgccagc gcggttttca aatacacacc ctcggggcag gtggcgggaa
     3541 cccgtttcaa ggggcagtgg cagacggtct ttttcgcttt cgggttcgag gccatctcca
     3601 gcacccgttc gggttacaac acccggatgg acgtgatgaa tgcggtgatg aactggtttg
     3661 atacgggcgc ggtctgcgcc cttttgggtg acgcaagcgg cgacagggtg gtggatattg
     3721 tggatttagt cgtcctggtc aactacgtgg tgtttgggtc accgatcccc accggcatta
     3781 actgcaccga gttgaccggc gacggggtca tcgacattct ggatatcatc tgcctgctca
     3841 attacgtcgt ctttgggaat ccgataccct gcggatgagg cagggggccg gcggagagcg
     3901 cttttttact gactttgggc aggagatttc gttgaaacaa tggaggtgtt actgaagaaa
     3961 agtagctgat gcggtaaaga cgatacttag tggcactatt atagtttcag atagagcgtc
     4021 cggtcactcc ttctgcagcg gtttctcgcg ccactccttg acggtagaat ctttttcaat
     4081 gtcgtagaga taataatgat attcgcgaat cccaatgatg ggtttagccg gtctattttc
     4141 atatttgggg tcagttatta cctcacggta ggttatcagc aaaaggtgac tgtctccgcc
     4201 ataccaggtc ggtcgaatag gcgtgtgctt gaaacgtttg taaaagcgat ccgtaatatt
     4261 catattgcgt tctcgctcga atacctgaaa agattcgtct ggatgtgatg agaggtagta
     4321 ttttccatct ggtgaaaagt ctataccgag atagggaact atttcagtaa ttccagtttt
     4381 tgtctcatac cggtagtacc tctgattcca cgaatagtag atattgctat cgaaaatagc
     4441 ccagttgaga aacaatgctg agtcagcaat tttcttaatt gattttgtca tcaagtcata
     4501 gattgccacg ccttgaggaa tcacccatct accttctgaa gttacaccct gaatgaaagc
     4561 cactttgcct gaagtcggtg accatgaaaa tttatggatg ttagatttta gtctatccgc
     4621 tacttgtccc tccggtctta tgatgaccaa tgaacgcatt tgattcttac cttcgagaaa
     4681 accaataaat ttcccattag gcgaagacaa aatgtcacta atgaaatccc cttccgcttc
     4741 gtaaatcaaa acccttgaag atgttagcgc gtcaactcta tagagacgac gattctcaat
     4801 cccgcttcct gatttgttcg ttagggcgtc accgcgaaca tacttctgcg gagaaacggt
     4861 ggttgttgaa gtagatgcac tggaaagcca agatgtggtg aaaactaaaa aagcctctcc
     4921 aattaccaga acaattaagt ttttcttcat atttatccct tttggttcct gtaccagcgt
     4981 ggtctctaat ttgaatagga gatgttagtt aagttttccc acccccggca cttgtcaatc
     5041 tttttcaaaa aaggaaacgg tctctatcct ctctttgcgg ctcacaccga atacacagcc
     5101 gcaagaacta ctacggaaac tgtggccaga aacttcctca tgcactggcc ttttaccccg
     5161 tttttatctt agcttaggtt tccgtttctt tttcttcctg caggccgtac tgcttcatct
     5221 tgacgcgcag ggttgattcg gggatctgga gggcactggc ggcgcgtttt ttaatccagc
     5281 ggttttcgga gagggctttg aggaggtagc ggcgctcgaa ctgggatatg tgctcgtaga
     5341 gggaaaagga gccgttggtt gttttgccta acccgtcgcc atccgggccg aagaatttcc
     5401 ccgaaagcag atcgggagtc aggcgggtgc cttcgccgag caacagaagc actttgcgga
     5461 tttcattctc caactcgcgg acgttgcccg gccaatcgta ttccgtcaaa agccgcagga
     5521 aggcagggtc gatgcgcggg agagtcgtgg caccggcggg ggagtgtttt ttcacaaagt
     5581 gttcgactaa aaggggaagg tcttctttcc gctcgcgcag gggcggaagt ttgaacgtga
     5641 gggtgcacaa ccggtagtag aggtcggccc ggaaaagctc tttttccatt tgcgtcttca
     5701 ggtctttgtt cccggcggag atgacccgca cgtcgacttt ctggggccgg gtgtcgccca
     5761 gccgggtcac ttctttttct tccaaaaccc gcaagagttt tacctgaatc gcagggggca
     5821 gttcggaaac ttcgtctaag tagaaagtgc cgccgtcggc ttcggcaaag aggccgatct
     5881 tgtcacgatc cgcgccggtg tacgcccctt tgcggtggcc gaagagctcg ctttccaaaa
     5941 gcgtttcggg caaagccgca cagttcaccg aaacgaacgg cttgtcccgt ctggcgctcg
//
LOCUS       DBNGZA010000053         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2024590, whole genome shotgun sequence.
ACCESSION   DBNGZA010000053 DBNGZA010000000
VERSION     DBNGZA010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2024590"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1008)
                     /locus_tag="AC16FU_01125"
     CDS             complement(178..1008)
                     /locus_tag="AC16FU_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993013.1"
                     /translation="MSIFQKLLTIDRRWIFLAIAVAVTTPFFFYIGLPIKVTPEVKAL
                     YDAVEQLKPGDVVVFAGEYDPATLPELQPMAQSFLRHCFSKNVKIIATGLFQLGVAQV
                     EQDLTLIAKEYGKIRGVDYVFLGYKPYPETVILNMGENFRKSFPTDYYGTPVDSLPMM
                     KGIKNYSDTKMIVSINATSGADYWIRSAHGRYKIPLGLGVTAVMATDYYSYLQSDQIF
                     ALVGGMKGAAEYERLAGFKGKKYLRATARMDIQNFAHLAIVFFVLLGNIAYFATRGKK
                     "
     gene            complement(1005..1670)
                     /locus_tag="AC16FU_01130"
     CDS             complement(1005..1670)
                     /locus_tag="AC16FU_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993014.1"
                     /translation="MKKEIPLAIMFITGVFMAVQFFIPHPTLAMIYQDFNSTVQVVGA
                     VALVLGIFSILSIHVTKIGKRQPGYGYSYFLIVFFFLMAASGFVPAGLQMGSFLGHGG
                     REEGSLFLNFYNYVLSPVQSSMFALLAFFIASAAFRAFRARSVIATLLLSTAIVVMLG
                     RVPIGPAMFGDWIADLANWIMAVPNAAAKRAIIVGVGLGILSLSLKMVLGIERSWLGG
                     REG"
     gene            complement(1667..2866)
                     /locus_tag="AC16FU_01135"
     CDS             complement(1667..2866)
                     /locus_tag="AC16FU_01135"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2993015.1"
                     /translation="MQTRKPGRLVRSLLPKALWIFFIFLFPSPSFAQAPAPPTNLQAK
                     DTPSDAGKSITITWQKSADDGAGANTVVSYEILRAAAPESAFASAGTVPAGKEQFVDN
                     GVENRRPYVYKIKAQTAGGTAGESLVSSPVAAKPQWWNSTPDYNRIFVGMVFVVGFIL
                     YNIRKAGKKELYIRPIAGINAIDEGIGRATEMGRPILFVCGLGTAADAATIAAFSVLG
                     RVSQKVAQYQSKLIVPCYDPIVFTVCQDVVKGAYSNAGRPEAYNEKDVFFVTQDQFPY
                     VAAVNGIMLREKPATNFYLGQFYAESLILAETGFQAGSIQIAGTDQLIQMAFFIVACD
                     FTLIGEELYAASAYLSKEPEQVGSIKGQDWGKVVFTTLSLLGVISVFLESKIHLFVWF
                     RKLFMAQ"
BASE COUNT          673 a          811 c          827 g          575 t
ORIGIN      
        1 ggtttgaacg tgtcccggaa gaggagaatc acccagtagc cgttggatac ccccaccacg
       61 acgtgctccg cgattttgta gaacgggttg tctttgtaga ggaaggagaa tatcgccagg
      121 gtgaaaaagg cggcgatcgt catccagagg gcgtggagca ccgctgtctc gggcatctta
      181 ctttttcccc cgcgtggcga agtaggcgat gttacccaga agaacaaaaa acacgatggc
      241 taagtgggca aagttctgga tatccatccg cgcggtggcg cgcagatact tcttcccctt
      301 gaacccggcc agccgttcgt actccgccgc ccctttcatc cccccgacta aggcaaaaat
      361 ctgatccgac tggagatagg agtagtagtc ggtcgccatc accgcggtca cccccaaacc
      421 caggggaatt ttgtagcggc cgtgggccga acggatccag tagtcggcgc cggaggtggc
      481 gtttatcgaa acaatcatct tggtgtcgga ataattttta atgcctttca tcatgggcag
      541 agaatcgacc ggcgtgccgt agtagtcggt ggggaacgat ttgcggaagt tttcccccat
      601 gttcaaaatc accgtctcgg gatagggctt gtaccccaaa aagacgtagt ccacgccccg
      661 gatttttccg tactccttcg cgatcaaggt caaatcctgc tccacctgcg ccacgcccag
      721 ctggaagagt ccggtggcga tgattttcac gtttttcgaa aagcagtggc gcaaaaagga
      781 ctgggccatc ggttggagtt ccggcaaagt ggccggatca tattctccgg cgaagacgac
      841 cacatccccc ggtttgagct gctccaccgc gtcgtagagc gctttcactt ccggggtgac
      901 tttaatcggc aggccgatgt agaagaagaa gggggtggtg acggccacgg cgatggccaa
      961 aaagatccag cgccggtcga tggtgagcaa tttttgaaaa atgctcatcc ttcccgtcct
     1021 cccagccagc tccgctcaat ccccaggacc attttcagcg aaagggagag aatgcccaaa
     1081 cccacgccca caatgatcgc ccgcttggcg gcggcgttgg gaaccgccat aatccagttg
     1141 gccagatcgg caatccagtc gccgaacatg gccgggccga tcggcacccg tcccagcatg
     1201 acgacgatgg cggtcgaaag caaaagggtg gcgatgacgc tgcgggcccg aaaggcccgg
     1261 aaggcggcgg aggcgatgaa gaaggccaga agcgcgaaca tgctcgattg caccggcgac
     1321 aggacgtagt tgtagaagtt gagaaagagc gacccttctt cccggccgcc atgccccaga
     1381 aaactcccca tctggagacc ggccggcaca aagccggaag ccgccatcag gaaaaagaag
     1441 acgatgagga aataactgta gccgtacccc ggctggcgct tgccgatttt agtaacgtga
     1501 atggagagaa tggaaaaaat ccccagcacc agcgccaccg cacccaccac ctggacggtc
     1561 gaattgaagt cctggtaaat catggccagg gtaggatggg gaatgaaaaa ctggacggcc
     1621 ataaagacgc cggtaatgaa cataattgct aaaggtatct cttttttcat tgcgccatga
     1681 atagttttct gaaccagaca aacaagtgaa ttttgctttc caggaaaacc gaaatgaccc
     1741 ctaagaggga aagggtcgtg aacaccacct tgccccagtc ctgccccttg atggagccaa
     1801 cctgctccgg ctccttggaa aggtaggccg aggcggcgta cagttcttca ccaatgaggg
     1861 tgaagtcaca ggccacgatg aagaacgcca tctggatgag ctggtcggtg ccggcaattt
     1921 gaatcgaccc ggcctggaag ccggtttcgg ccagaatcaa cgactccgcg taaaactgtc
     1981 ccaggtagaa gttggtggcc ggcttttccc gcagcatgat cccgttgacc gcggcgacgt
     2041 acgggaattg atcctgggtc acgaaaaaga cgtctttctc gttgtacgct tccggccgcc
     2101 cggcattcga gtaggcaccc ttgaccacgt cctggcacac ggtgaagaca atcgggtcat
     2161 agcaggggac gatcagcttg ctctgatact gggctacctt ttgagagacc cgccccagga
     2221 cggaaaaagc ggcaatggta gcggcgtccg ccgccgtccc caaaccgcag acgaagagaa
     2281 tcggccgtcc catttcggtc gcccggccga tgccttcatc gatcgcatta ataccggcaa
     2341 ttggtcggat gtagagctct tttttcccgg ccttacggat attgtagaga ataaagccga
     2401 ccacaaagac catcccgaca aaaatccggt tgtaatccgg tgtgctgttc caccactgtg
     2461 gttttgctgc aaccggggat gagacgagcg attcaccggc cgtacccccc gcggtctggg
     2521 ctttgatctt gtacacatag ggacgacgat tttccacccc gttgtcaacg aattgctcct
     2581 ttccggctgg caccgtcccg gcgctggcaa aagcgctctc tggcgccgcc gcccgcagga
     2641 tttcgtagga gacgaccgta ttggcacccg cgccgtcatc cgccgatttc tgccaggtaa
     2701 tggtgatcga cttgcccgcg tcgctgggag tgtctttggc ctggaggttg gtgggaggag
     2761 ccggtgcctg ggcaaaagac ggagacggaa agaggaaaat gaagaaaatc cagagggctt
     2821 ttggcagtaa cgacctgacc agtctcccgg gttttctcgt ctgcattagc tggtccttcc
     2881 ctcctg
//
LOCUS       DBNGZA010000054         3336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2767406, whole genome shotgun sequence.
ACCESSION   DBNGZA010000054 DBNGZA010000000
VERSION     DBNGZA010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2767406"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1980)
                     /gene="gyrB"
                     /locus_tag="AC16FU_01140"
     CDS             complement(28..1980)
                     /gene="gyrB"
                     /locus_tag="AC16FU_01140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR2993016.1"
                     /translation="MPDVLDKRNVVIGHHYDATTIQVLKGLEAVRRRPAMYIGDTGTR
                     GLHHLVYEVVDNSVDEALAGFCDQIKVAIHKDSSVTVEDNGRGIPVDIHPTQKRPALE
                     VVMTMLHAGGKFDHQSYKVSGGLHGVGVSVVNALAEWCEVEVARDGQVFFQRYEQGNP
                     KGDVKVTGKRQKTGTITTFKADPEIFGKVEYSYDVLASRLRELAFLNANLQIDLADER
                     TGKKETFKYKGGVAAFVAYLNENKQPLFSKPLYFHKEKEGVDVEIAIQYNDSYVENLF
                     SYVNNINTIEGGTHLVGFKTALTRTINNYAEKNGFLQKVDFSIIGEDVREGLTAVISV
                     KVPDPQFEGQTKTKLGNSEVKGIVETITGEGLASALEETPAVARKIVEKTLEAARSRE
                     AARKAKELVRRKSALENSPLPGKLADCSLDDPEQCEIYIVEGDSAGGSAKQGRDRRFQ
                     AILPIKGKILNVEKASQDRVFSNEEIRAIITALGTGVGTGDGNGDTDTFDLSKARYGK
                     VIIMTDADVDGSHIRTLLLTFFFRRMKALVEQGKVYIAQPPLYRLKKGNTEMYAYSDD
                     ERDRLMERLGRQGVGVQRYKGLGEMNPDQLWKTTMDPETRTLKQVSLEDAAEADHIFA
                     ILMGEEVEPRRKFIEENARYVKNLDV"
     gene            complement(2047..2334)
                     /locus_tag="AC16FU_01145"
     CDS             complement(2047..2334)
                     /locus_tag="AC16FU_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR2993017.1"
                     /translation="MEKPELLSTILTQRLAALGLTKRVKAHQAVTLWPKVVGEKIAAE
                     SQAVRIEEGKLVVRVPKSVWRQELINLKPRLIAKINAVIGQAVVGDIFFTG"
     gene            complement(2493..>3336)
                     /gene="dnaN"
                     /locus_tag="AC16FU_01150"
     CDS             complement(2493..>3336)
                     /gene="dnaN"
                     /locus_tag="AC16FU_01150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR2993018.1"
                     /translation="TYRLSGVPAEEFPKLPEFNGSKEVKVPAQDLVQMVTKTVFAASK
                     DETRPALNGVLWQMSGDKMIMVATDGHRLAKIGVENKKLKGLPGNVIVPPKVLNMLER
                     LVGDQEKEIGLVFSDSHIVFNLGDSILTSRLIEGPYPNYEQVIPKNNDKRLSVPRDQM
                     TAAVRRVAILSNSLTHQVKLTLKKSQMELSASNADVGGDAREVIPCSFSGDEMEIGYN
                     AQYVLDTLKQFDSDEVVFDLATPVSAGTIHAGSVPTRAEEKKPADAPQADYFCLLMPL
                     RLTE"
BASE COUNT          623 a          991 c          903 g          819 t
ORIGIN      
        1 atcctcctct gtatctcttt ggggtagtta tacatccaaa ttcttcacgt atcttgcatt
       61 ttcctcgatg aacttccgcc tcggctcaac ttcttcaccc ataaggatgg caaatatgtg
      121 atcggcttcg gcggcatctt cgagggagac ctgtttcaac gtccgggttt ccgggtccat
      181 ggtggtcttc cacaactggt ccggattcat ttcgcccaac cccttgtagc gctggacacc
      241 caccccctgc cggcccagcc gttccatcag ccgatcgcgc tcatcgtcgg agtaggcata
      301 catctcggta ttgccctttt ttagtcgata caagggcggc tgggcaatgt agactttccc
      361 ctgctccact aaggccttca tccgacggaa gaagaacgtc aggagcaggg tgcgaatgtg
      421 ggagccgtcc acgtcggcat cggtcatgat gatcaccttg ccgtagcggg ctttggagag
      481 gtcaaaggta tcggtgtcgc cgttgccgtc gcctgtcccc accccggtcc ctaaagcggt
      541 aatgatggcg cggatttctt cgttggagaa aacccggtcc tggctcgcct tctcgacgtt
      601 taagattttt cccttgatcg gcagaatcgc ctggaagcgc cggtcgcgtc cctgcttggc
      661 cgagccgcca gctgaatcgc cttcgacgat gtagatttcg cactgctccg gatcgtccaa
      721 cgaacaatcg gccaacttgc cgggcagagg cgagttttct aaggccgatt tgcgccggac
      781 cagctctttg gcttttcgag ccgcttcgcg ggagcgtgcc gcctccagtg ttttttcaac
      841 gattttgcgg gccaccgcgg gggtttcttc gagggcgctg gctaaaccct ccccggtgat
      901 cgtctcgaca atccccttca cctcactatt gcctaacttg gtcttggtct ggccttcgaa
      961 ctgcgggtcg ggcaccttca ccgaaatcac cgcggtcagc ccttcgcgca cgtcttcgcc
     1021 gatgatggag aagtcaacct tctgcagaaa gccgttcttc tcggcgtagt tgttgatggt
     1081 gcgggtcaaa gcggtcttga agccgactaa atgggtcccg ccctcaatgg tattgatgtt
     1141 gttgacgtag gagaaaagat tctcgacata ggaatcgttg tactgaatgg caatttcgac
     1201 gtccacgccc tctttttctt tgtggaagta aaggggcttg gagaaaaggg gctgtttgtt
     1261 ttcgttcagg tacgctacga aggccgccac cccgcctttg tacttgaatg tttctttctt
     1321 cccggtccgt tcgtcggcca gatcgatctg gaggttggcg ttgaggaagg ccaattcccg
     1381 cagccggctg gctaagacat cgtaactgta ttcgaccttg ccgaaaattt ccgggtcggc
     1441 cttgaaggtc gtaatggtgc ccgtcttctg ccttttgccg gtgaccttga catccccttt
     1501 ggggttcccc tgctcgtagc gctggaaaaa gacctgcccg tcgcgggcga cctccacctc
     1561 gcaccattcc gcaagagcgt tgactaccga gacgccgact ccgtgcaatc cgccggagac
     1621 tttgtaggac tggtgatcga acttgccgcc ggcgtggagc atcgtcatca ccacctccag
     1681 agccggccgt ttctgggtcg ggtggatatc gaccggaatc ccccggccgt tgtcctccac
     1741 cgtaacgctt gagtccttgt ggatggcgac tttgatctgg tcacagaaac cggccaaggc
     1801 ctcgtccacg gagttatcca ccacttcgta gactaagtgg tgcagacccc gcgtgccggt
     1861 atcgccgatg tacatcgccg gccggcgccg gaccgcctcc aggcccttca gcacctggat
     1921 ggtggtggca tcgtagtggt ggccgatgac tacattcctc ttgtcaagga catcgggcat
     1981 tgattcccct ctcgtagccg agtcgtaccg atcctgccgc accggatgat ttttctccca
     2041 gcaacgtcat ccggtgaaga atatgtcccc caccactgcc tgcccgatga cggcattgat
     2101 cttggcaatc agtcggggct tgagattgat caactcctgc cgccagaccg acttcgggac
     2161 cctgaccacc aatttccctt cttcgatccg cactgcctgg gattcagcgg cgattttctc
     2221 cccgacgacc ttcggccaga gcgttaccgc ctggtgggct ttaacccgtt tggtcaaccc
     2281 cagagctgcc agccgttggg tgagaatagt gctcaaaagt tccggttttt ccataaaggg
     2341 ataacgaccg ttccggtcgt atcttcacgt ttataaaatc tacggaaatt ggcggcttta
     2401 cgcaagaaga accggggcgg ggagaccccg cccctacgca cccgggaggg cgacccgtac
     2461 gggtcgccca gttactgatc cgttcgggac agtcactccg tcaaccgcaa cggcattaac
     2521 aagcaaaaat aatctgcctg aggggcatcg gcgggtttct tctcctcggc tcgggtcggg
     2581 accgatccgg cgtggatcgt acccgccgag accggcgtgg ctaagtcaaa caccacctcg
     2641 tcggaatcaa actgcttgag tgtatccagt acgtactggg cgttgtagcc gatctccatt
     2701 tcgtctccgc tgaacgaaca ggggattacc tctctggcat ccccccccac gtcggcgttg
     2761 gaggccgaaa gttccatctg cgatttcttc agggttaatt tcacctgatg cgtgagtgaa
     2821 ttggagagga tagccacccg ccgtaccgcg gcggtcatct ggtcccgcgg cacgctcaag
     2881 cgcttgtcgt tgttcttcgg aatgacctgc tcgtagttgg gatacgggcc ttcaatgagc
     2941 cgtgaggtca ggatagaatc gcctaagttg aacacgatgt ggctgtcgga gaagaccagc
     3001 ccaatctctt tttcttgatc cccgacgagg cgctccagca tattcaacac ttttggcgga
     3061 acaatcacgt taccaggtaa gcctttgagt tttttgtttt ccaccccgat tttggccagc
     3121 cggtggccgt cggtggccac catgatcatt ttgtcgccgg acatttgcca gaggacaccg
     3181 tttaaggccg gtcgcgtctc gtcttttgaa gcagcaaaga ccgtcttcgt gaccatttgc
     3241 accaaatcct gcgcgggaac tttcacctct ttgctgccat tgaattcggg taatttcgga
     3301 aattcttcgg ccggcacccc ggagagccgg tacgtt
//
LOCUS       DBNGZA010000055         1590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1706468, whole genome shotgun sequence.
ACCESSION   DBNGZA010000055 DBNGZA010000000
VERSION     DBNGZA010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1706468"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="AC16FU_01155"
     CDS             <1..1315
                     /locus_tag="AC16FU_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2993019.1"
                     /translation="ATKERAPVPQTRPRVHADRPAGFMKAIVKEKPKEGQPWPRGLHL
                     VEKAVPKVLHPDDVKVEVIAAGICGTDVGIYNSKDSIRVEMAKLLQDSVTIGHEFCGR
                     VVDAGEGARLYFARQLADRHWTGDDDPALLRLVQSRSPDAIAADSHLIDLLGQHFLLS
                     AEMHQTCGHCYQCRRGERHVCQNTVIKGIHDDGAFARYMLTSGRNIILFEKDELPVEI
                     IAFLDAFGNAVHTAQATRLEGRTVAVLGCGVQGLMSTAIARYCGAAKIFATDAANPEK
                     GLTPEKLAKKRFTLAAKLGADCCFDVGTPQGHGEFVEAVKAETHGTGVDVVFEMSGSY
                     NAYRDAFDVVRMGGTVALLGIPEGEWPLDFSKKVIFPGVTIQGIIGRRVFETWEVMRA
                     MLKAGVAQQLLGCGFVSHQFPLEKFEEGFAVICSGDGLKVLLRPT"
BASE COUNT          342 a          431 c          491 g          326 t
ORIGIN      
        1 agccaccaaa gagcgagcgc cggttcccca aaccaggccc agagttcatg cggatagacc
       61 cgcaggtttc atgaaggcca tcgtcaaaga aaaaccaaag gaggggcagc cctggccgcg
      121 aggtctgcat ttagttgaaa aagcggtccc aaaagttctg catcctgacg acgtcaaagt
      181 cgaagtgatc gccgccggca tctgcggcac cgacgtcggc atctacaaca gcaaagactc
      241 catccgggtc gaaatggcga aattgctcca agattcggtc accatcggcc acgaattttg
      301 cgggcgggtg gtggacgcag gcgaaggggc acgtctctac tttgcccgcc agttggccga
      361 ccgccactgg accggcgacg atgatccggc ccttttacgt ttagtccaga gtcgatctcc
      421 ggatgccatc gccgccgatt cgcatttaat agatctgctc ggtcagcact tcctcctttc
      481 cgccgagatg catcagactt gcggccattg ctaccagtgc cggcgcgggg aaaggcacgt
      541 ctgccagaac acagtgatta aggggatcca tgacgacggg gcgttcgccc ggtatatgct
      601 cacctcgggg cgcaacatta ttctttttga gaaagacgaa ctgccggtgg aaatcattgc
      661 tttcctggat gctttcggca acgcagtcca cacggcgcaa gcgacccgcc tggaaggccg
      721 gacggtagca gttctggggt gtggcgtgca ggggttgatg tccaccgcga ttgcgcgtta
      781 ctgcggagcg gcgaaaattt ttgccaccga tgcggccaat cccgaaaagg ggttaacacc
      841 ggagaaactg gccaagaaac gcttcaccct tgcggccaaa ctgggcgccg actgctgttt
      901 tgacgtggga accccccagg ggcacggtga attcgtcgag gcggtcaagg ccgagaccca
      961 cggcaccggc gtggatgtgg tctttgagat gtccggttcc tacaatgcct accgggatgc
     1021 cttcgacgtg gtgcggatgg gcggcacggt ggcgcttttg ggtattcccg aaggggagtg
     1081 gccgctcgat ttctccaaga aggtgatctt ccccggggtg acgattcagg gaatcatcgg
     1141 gcggcgggtg ttcgagacct gggaagtgat gcgggcgatg ctcaaagccg gggtggccca
     1201 gcagttgctc ggttgcggct tcgtctccca ccaattccct ttagaaaaat tcgaagaagg
     1261 ctttgctgtc atctgctccg gggatgggtt gaaggtgctt ttgcgaccaa cgtgattgga
     1321 gattttgtaa aatttgcagc gcgggatggt tctgaatccc gcgctatttt tcgcgcaggt
     1381 tacacccttc ggcttcgctc agggcaagcc aacctgcgct acggttcaga gaggaccgct
     1441 gagttaactc attaacgaga aaaagcaggg ataaacgatg atcggaacag tccgggattt
     1501 tctcactaag gaactcaccg gcattcgtga agcggggctc tacaaggaag agcgggtgat
     1561 cctcagcccg cagggggccg agattcgcgt
//
LOCUS       DBNGZA010000056         1246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1388931, whole genome shotgun sequence.
ACCESSION   DBNGZA010000056 DBNGZA010000000
VERSION     DBNGZA010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1388931"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="AC16FU_01160"
     CDS             <1..1135
                     /locus_tag="AC16FU_01160"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR2993020.1"
                     /translation="DERFIFFARGTLEAVKQLGWQPDLIHCNDWQTALTPGYLKTVYR
                     NDPFFAQTRTLLTLHNVAYQGNFPKETFVKLGVDPGLFHPLGPFEFWGKVNFLKAGIV
                     YADLLSTVSERSAVEIQADAEFGYGLEGVLRQRNADLFGILNGVDYQEWNPATDQYIA
                     SRYTTKSLSKKKENKKALLKRCGWKATTLKQPLIGAISRLVDQKGFDLVADILEELLA
                     GGARFVLLGTGERRYHELFLRFAQTYPDRMKLFLGFDEPLAHLIEAGSDMFLMSSRYE
                     PCGLNQMYSLKYGTVPIVRETGGLADTVQEFSPGSTNGAGTGFVFRHYDAGELLDAVR
                     RALKVYEDQTAWKRLMLAGMTKDYSWETSAKKYFNLYDRAKTR"
BASE COUNT          258 a          361 c          375 g          252 t
ORIGIN      
        1 tgatgaacgg ttcatctttt ttgcccgggg gacgctggag gcggtcaagc agctgggctg
       61 gcagccggac ctcattcact gcaacgactg gcagacggct ttgactcccg gctacctcaa
      121 gacggtctac cggaacgatc ctttctttgc gcagacgcgg acgcttttga ccctgcacaa
      181 cgtggcctac caggggaatt tcccaaaaga gacgtttgtc aaactgggcg tcgatccggg
      241 cctgttccac cccttaggtc cgtttgagtt ctgggggaaa gtcaacttcc tcaaagcggg
      301 gatcgtctac gctgatcttc tctccacggt gagcgagcgg tccgcggtgg aaatccaggc
      361 cgacgctgag ttcggatacg gcctggaagg ggtactgcgc cagcgcaacg ccgacctctt
      421 cgggatcttg aacggagtcg actaccagga gtggaatccc gccaccgacc agtacatcgc
      481 cagccggtac accactaaat cgctttccaa gaaaaaggag aacaagaagg cgctcctgaa
      541 gcggtgcggc tggaaggcca ccacactcaa gcagccgctc atcggtgcca tttcccggct
      601 ggtggaccag aagggattcg acctggtggc cgacatctta gaagagcttt tggccggagg
      661 agcgcgcttt gtgctcctgg gtacgggtga gcggcgctac catgagctct tcctgcgctt
      721 cgcccagacg taccccgacc ggatgaaact ttttttgggg ttcgacgaac cgctggcgca
      781 cctgatcgag gccggcagcg acatgttttt gatgtcctcc cgctacgagc cctgcggact
      841 gaaccagatg tacagtttga agtacggaac ggtgccgatt gtgcgggaga ccgggggctt
      901 agccgatacg gtgcaggaat tctcacccgg ttccaccaac ggcgcgggaa ccggtttcgt
      961 gttccgccac tacgatgccg gagagctttt ggacgccgtc cggcgggcgc tcaaagtcta
     1021 cgaagatcaa acggcctgga agcggctgat gctggccggc atgaccaagg actactcctg
     1081 ggagacttcc gcaaaaaaat acttcaatct gtacgacaga gccaagacac gctgagctct
     1141 gcggcagcgt tttacgcagt ttccaagcgg taaccggctt tcggctatgc agaccgataa
     1201 gcgggcacct ttccttgtgg ccctggtgta cgaagcccgc ggtgat
//
LOCUS       DBNGZA010000057         3887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_434458, whole genome shotgun sequence.
ACCESSION   DBNGZA010000057 DBNGZA010000000
VERSION     DBNGZA010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_434458"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1783)
                     /locus_tag="AC16FU_01165"
     CDS             complement(266..1783)
                     /locus_tag="AC16FU_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2993021.1"
                     /translation="MEGRAALKRLSIFGLAAIFSLAFPFSSASSQTSLLYRFDGSALD
                     ELGYSVAEVGDVDGDGKADFIIGVPRADPNGLTDAGSAFVYSGATGALLYRVNGAADF
                     DFFGFSVAGAGDIDGDSRADFIAGAPFASPEGIFIAGSAFVYSGATGVLLYQKNGEPG
                     DRLGHSVAGGGDMDGDDRADFIVGAYFADPGGMVNAGSAYVYSGADGGLLYHKDGTAA
                     TDRFGQFVAVTGDLNGDGPADFIVGAPLTDPGGLADAGSAYVYSGADGHLLYRINGAS
                     AGDGLGVPVAGTGDLNGDGQAEFLVGATFADPNGLADAGSLSVYSGKDGSLLFQENGV
                     SAGGRLGFAVSGAGDLDGDGVPDFMAGAPFADPGGLEDAGSAFVFSGANGLLLFQKNG
                     TSGDQLGWAVAGSGDLNGNGSAGFIVSARYADPGGLVDAGSVFVYQCLPRGDVNNDGE
                     TDVMDIVGLIQHVVFDVSLNSLIAGDTNCDGLSEIVDIVLLIEYLVYGTPALCCL"
     gene            complement(2163..>3887)
                     /gene="feoB"
                     /locus_tag="AC16FU_01170"
     CDS             complement(2163..>3887)
                     /gene="feoB"
                     /locus_tag="AC16FU_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR2993022.1"
                     /translation="ARLGVPVVAVVASRQTGLPELAQAVKKSVFGQWQSPERFWRLLP
                     AFENRVKTYAPHAAATELETVACVAGLHTPACTEELEKVAQQIRSELGVNEETVRRYI
                     VEARYRWQREVTREVVEKTGSSRTDWTKKLDKVFLNPLLGPLVLLATLTLVFQSIFTW
                     AQAPMNAIGYVTGTFLPSLTYKILAPGPLSSLINDGIFAGVGAVVTFLPQILLLFFFL
                     TFMEDSGYLARAAFLLDRLMSKVGLSGRSFIPLLSSFACAIPGIMATRTVPHHRDRLA
                     TILVAPLMTCSARLVVYTLLIAAFIPVAHVWGPFSLQGMTLLGLYLLGIFAAAGVAAL
                     FRRTILRGPTPPLLLELPPYRWPKPQTLFLTLWDRAKIFLVSAGTIIFAISVLLWGLS
                     SYPRWTSIESHLTAGQKILFSQPDGPQKQAALEALNQKLAAERLEHSFAGYLGKAIAP
                     VLQPLGFDWKIGVGILGSFAAREVFVSTMGVVYGVGSAAEGQTLTDRLKADRSPVTGN
                     LVWTPLVAITLLVFYAFALQCMSTIAIIRRETNTWRWPAFAFAYMTVLAYGGALATRW
                     IGQAFFGG"
BASE COUNT          924 a         1208 c         1064 g          691 t
ORIGIN      
        1 agaggatcga atcgttgtgg agcaaaatat ctatggtgcc cgtgctcccg gggctgaaga
       61 ccctggaaac cacaatgacc gaatcgacaa acggcccggc tatcccctcc cgcgttgccc
      121 ctgcgcagag gagcaaaatt cccgcactga caatgctcaa gcgtttcatt ttaccctccc
      181 ttctccggtg ttctgctctc tctgacattt cgatatgatc tctgtcccgc cgcccgcgaa
      241 aaccgcgcta ctttgctccg acctttcaca agcagcacag ggcgggtgtc ccgtacacca
      301 ggtattcgat caaaagcacg atgtccacaa tctcagataa cccgtcgcag ttggtatctc
      361 cggcaatcaa actgtttaag gaaacgtcga acaccacatg ctggatcaac cccacgatgt
      421 ccatgacgtc ggtctcgccg tcattgttca catccccgcg gggcagacac tgatagacaa
      481 ataccgagcc cgcatccaca agcccgcccg ggtcggcgta gcgcgcactg acgataaaac
      541 cggcgctgcc gttgccattc aaatccccgc tcccggccac cgcccacccc agctggtcac
      601 cggacgtgcc gtttttctga aaaaggagca acccgttcgc cccggaaaag acgaatgccg
      661 agccggcgtc ttccagcccg cctggatcgg caaacggagc accggccata aaatcaggta
      721 cgccatcgcc gtctaaatcc ccggccccgg acaccgcaaa gcccagacga ccgccggcgg
      781 aaaccccatt ttcttgaaag aggagcgaac cgtccttgcc ggaatagacc gagagggaac
      841 ccgcatcggc cagcccgttc ggatcggcaa aagtagcacc tactaggaac tctgcctggc
      901 cgtcgccatt cagatccccc gtccctgcca ccggaacgcc gaggccgtca ccggcgctgg
      961 caccgtttat ccggtaaagc aggtggccat cggctcccga atagacataa gccgagccgg
     1021 catccgccag accgcccgga tcggtaaggg gagcgccgac tataaaatca gccgggccgt
     1081 cgccattcag atcacctgtt acggccacaa attgtccgaa ccggtcagta gccgccgtcc
     1141 cgtctttgtg gtaaagcaaa ccgccgtccg cccccgaata aacataggcc gaaccggcat
     1201 tgaccattcc gccgggatcg gcaaagtatg ccccgactat aaaatcggct ctgtcatcgc
     1261 catccatatc tcccccgccc gccaccgagt gtcccagccg gtcccccggt tcgccgttct
     1321 tctggtaaag cagaactcct gtggcgccgg agtagacaaa tgcggaaccg gcgatgaaaa
     1381 ttccctccgg acttgcaaag ggagcgccgg cgataaaatc ggctcgactg tcgccatcaa
     1441 tgtctccggc tcccgctacg gaaaaaccga aaaagtcaaa atcggcggcg ccgttcaccc
     1501 gatacaacaa agctccggtt gcgccggaat agacgaaagc cgagcccgca tcggtaagcc
     1561 cgttcgggtc ggcccgggga acgccgatga tgaaatcggc tttgccgtcg ccgtctacat
     1621 ctccgacctc ggccacggaa taacccagtt cgtcaagggc ggagccatcg aaccggtaga
     1681 gcaaactggt ttggctggag gcggatgaaa aaggaaaggc gagggagaaa atagcggcca
     1741 gtccgaaaat actaagccgc ttcagggctg ctctcccttc catgtaatca cttgaataac
     1801 acattcgttc cgacacacct ctccctttcg gacaccggtg gcgtgtccct gcccctcccg
     1861 ccaataaaat agccatctcc attttttctg tcaagccccg tcgccgagga tttggtagac
     1921 tggctcgccc cggagccggt cgggagtgta agttctgctc ccgattaacg aaaagtctgc
     1981 tttttcttgg atgtcctcgc ggtgtcaagc aagttcgggc cgctgcaccc ggattgacaa
     2041 gccggacaag aagcgttctt tttgctcctc tttttccaga agaggaaaag ccccgcgcca
     2101 acaaagaatg atgcgacgga aatatcttga accaccatcg gcaagttcac cggaaacatg
     2161 ctttatcctc caaagaatgc ctgcccaatc caacgagtgg ccaaagcacc gccataggcc
     2221 agaacggtca tataagcaaa ggcaaaagcc ggccaccgcc aggtattcgt ctcgcggcgg
     2281 atgatggcaa tggtggacat acattgcaac gcaaaagcgt agaaaacgag gagagtgatt
     2341 gcgaccagag gtgtccagac caggttgccg gttacaggtg agcggtcggc tttcagacga
     2401 tctgttaacg tttggccttc cgccgcgctc cccacgccat agaccacgcc cattgtggaa
     2461 acgaaaactt cgcgcgccgc aaaagagccg aggatgccca cgccgatttt ccagtcaaat
     2521 cccagtggtt gcagcaccgg cgcaatggcc tttcccagat acccggcaaa agaatgctct
     2581 aaccgctccg ccgccaattt ttgattgagc gcctccagcg ccgcctgttt ctgtggaccg
     2641 tcgggctgag agaataaaat cttctgccca gcggtgaggt gactctcaat cgatgtccag
     2701 cgcgggtaac tggaaagccc ccagaggaga accgaaatgg cgaagatgat cgtccccgct
     2761 gaaacaagga agattttcgc ccggtcccag agggtcaaaa agagcgtctg cggcttcggc
     2821 cagcggtagg gcggcagttc caaaagcaaa ggcggcgtcg gcccgcgcag aatcgtccgg
     2881 cggaatagtg ccgcgactcc ggcggcggcg aatattccca gaaggtacag gcccaaaagc
     2941 gtcatcccct gcaagctgaa cggcccccag acgtgtgcaa ctggaatgaa cgcggcaatc
     3001 aaaagggtat acaccaccag ccgcgcggaa caggtcatga gcggcgcaac taaaatcgtc
     3061 gccagccggt cgcggtggtg cggaacggtc cgggtggcca taataccggg tatggcgcag
     3121 gcaaaggaag acaggagcgg aatgaaactg cgtccggaaa gcccaacttt ggacatcaag
     3181 cggtcgagga gaaacgcggc gcgggccaga tagccggagt cttccataaa ggtgagaaag
     3241 aagaagagca ggagtatttg cggcaggaaa gtgacgaccg ccccgacgcc ggcgaagatg
     3301 ccgtcgttaa tcaaactgga aagcgggccg ggagcgagga ttttataagt gagcgacggc
     3361 aaaaacgtcc cggtgacgta gccgatggca ttcatcggcg cctgcgccca ggtgaagatc
     3421 gactggaaaa ccaaggtcaa cgtggccaac aaaacgagcg ggcccaagag ggggttcagg
     3481 aagaccttgt ctaatttttt ggtccaatcg gtccgcgaac tgccggtctt ctcgacgact
     3541 tcccgggtca cttcccgctg ccaccggtag cgggcttcca cgatgtagcg cctgaccgtc
     3601 tcttcattca cccctaattc ggagcggatt tgctgggcga ctttttccaa ctcctcggtg
     3661 caggcggggg tgtgcagtcc ggccacgcag gccaccgttt ccaattccgt cgctgcggcg
     3721 tgaggagcgt aggtcttcac ccgattttca aacgccggca aaagccgcca gaagcgttcc
     3781 ggtgattgcc attggccgaa gacgcttttc ttgaccgcct gagccaactc cgggagcccc
     3841 gtctgccgtg aggcgaccac tgcaacgacc ggaacaccca aacgggc
//
LOCUS       DBNGZA010000058         9855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_540780, whole genome shotgun sequence.
ACCESSION   DBNGZA010000058 DBNGZA010000000
VERSION     DBNGZA010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_540780"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC16FU_01175"
     CDS             complement(<1..569)
                     /locus_tag="AC16FU_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993023.1"
                     /translation="MLSGFVLWGFAPLFPLGTADGQTRTPTHLERFEPGVPTCDSIEI
                     IGCLVLCPAGDQPFQVRLRDQFGNPVSGWPKENVWLNFSDCPSICPCPDEAAWPLVYP
                     EDTSNAGGIVTFRIGAGGCRDHCTITLDIVRAVGDTCKLTVNLVRTTDFNCDLTVSFE
                     DVCIMKNCFFQGICLPDPLCFDFNCDGIN"
     gene            complement(751..3360)
                     /locus_tag="AC16FU_01180"
     CDS             complement(751..3360)
                     /locus_tag="AC16FU_01180"
                     /inference="COORDINATES: protein motif:HMM:NF049220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7451 domain-containing protein"
                     /protein_id="HIR2993024.1"
                     /translation="MRKRDLIGPVILLAAGLVCNALGAEQKPNSDASQPKNTALQVTP
                     VGKTGWAPSKQLTGPQDVCPFTMACFSGQVAFTVSGLPGGELCEYQDPAKTCPPPAAC
                     PTYPFEVKNVHILFSNANATACVLLVTPGIRRADFTNPACPVPGAMICTGPVQQIVIP
                     PNTQCMELIFPLNPGCCVDTTYFACLNFQVNCPPPGVGPCLDNAPCDPCCSYWDQTTG
                     TFIDWCTVIPSGGNLMLWTDGTLSCQNNCPRRPTGKNHYKTWRVLPNPTTILSATVSD
                     QFIQNDPVRLDSIQYLSNPTRKVTINPTRNDTFNIVDTTQHLSWYKAIGRDLLLQVRY
                     GNQFETTAVVIDSMRYLLVPTQKQPHGPPQGLDHYKAYRIKNPTAFSRQVQLQDQFDG
                     ANPELIQQLVPVYFLTPCRKNADPFFDDTTHYVAYRIAPQRTTTQQRQTQDQFGMHVL
                     TSVVSELLLVPTKKLSFGPPPPPECSDGLDNDGDGLIDFPADCGCINPSDNTEAPNPV
                     TQCNDGIDNDGDGLIDLADCGCKDVCDNSESPDAVTQCNDGIDNDGDGLIDFPADPGC
                     ANACDNDEFNPPPTTRKNHYKTWRLLPNPTQFLTAQVRDQFIQNDFLRLDSIQYLSNP
                     TQKITINPTRNDTFNIVDTTQHLSWYRARGLDTLLTVRYENQFESTTVQIDSVRYLLV
                     PTQKQPHAPPQGLDHYKAYRIRNGVPFTRPVQLQDQFDPVPEQIQTLVPVYFLTPCIK
                     NADPFFDLTTHYVAYSIFPPRTITQTRGTFDQFGPHVLTSIESDFLLVPTTKIGFCPS
                     IPGDLTGDGVKDIVDIVALINCVVFNDCPFANPPCCDLNCDGICDIVDIVCLINCVVF
                     VTQCTGCPFNWCP"
     gene            3838..5880
                     /locus_tag="AC16FU_01185"
     CDS             3838..5880
                     /locus_tag="AC16FU_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993025.1"
                     /translation="MKKDPPVPAPAGDDPLPVSSPVTIWTGPESAMHDPLSTIHKEPE
                     RPETTKVQLDFSGDDPTPDRQATPTEPAERLELESPIAKVEASSRSIGPHPQDELAAQ
                     LVENLDQKKTGSQSSRKKKSPVTNRESDIYHISNYSVGTNALEVQRGRKENAEPPLPL
                     SVIRSDEATPRSGSDLVIQTEPPRRPRRAPEDPTSGEAGDRHERYPGLKDIAYFSGST
                     VRFTGGVRLHSGDEIAIGDRSFILRAKGTSKLPIVLAGAVAGVLALAALIYSLVGKPT
                     PPSVLSGVVYDRASKQLVGGVGIKIAELGKTVGTDELGYFRFDDLAPGTYTLEALTPD
                     GSAIRKKVEIQSEKSGFVTLAVPARKSPLPLLGSPDQPPPNLARTVPEAGRTPRASQK
                     GNLKIAVSVPNATVRLDGKLLGVGNTTYVNVKSGSHRVAISKMGYKPWQGIVDIPQGG
                     TTTISAALESDQEGPAKSLSFDDYLKSAQKKSAAGDHSGAVADYEKALNLSPNNPLAL
                     AGRGVELAETGDRAKALNDFRQAGSLFFSDRKYGLAAENFSRALDIAPSDGDLLLRRA
                     QSYFRAGQLRESREDYKALAKQAPNSAQAYAGWGLCEFQFGNMKEAIKVFEKATQLAP
                     TTAEYYGYLTMAHFESGNRDKARKYYTKFSDFANLLDKQAFEKYPTWQMVTRAFGS"
     gene            complement(6056..8182)
                     /locus_tag="AC16FU_01190"
     CDS             complement(6056..8182)
                     /locus_tag="AC16FU_01190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR2993026.1"
                     /translation="MSTADLSAIGNLLGERLSTVLGCSGFGFFWRETPADGFRIICAQ
                     TPPELPERFWEIVAAETFSVRRAANQEPITWGTGGTGELSSDLQQIFQVTAVLTVPVT
                     DNGYTQAALAAIWHRAPHQFGETDLVLARLLGDFSRAELCRLRLGRELEKGNQRLTAL
                     SELATTMYSSLNHQVVLEKLLALATKLARADGGTIYLCDKEAGLLQPLMTNREPYREQ
                     IMAFTIPLGEGLSGKVAQTGVGQILNHAEKDETAVQVPDTPLEVESLISVPLLYAGDV
                     IGVITLTALGGREFQSEDLELLTIFARQAADVLEMARLYKNLEQAYQQLFTAQEQMVQ
                     TEKLRALGEMAGGVAHDFNNMLGAVLGRVQLLLTQAQDEKTRLGLEQIQELTLAGART
                     VARLQEFTRVKTDAAFTAVDLGQVIREAIEVTKPRWRDQVQESGGFIAIENRLEPVPP
                     VQGNAAELVEVFTNLISNAVDAMPAGGTLTFTSRLDGERVSVIVTDTGQGIPEEIKSK
                     IFFPFFTTKGVKSTGLGLSVSYGIISRHKGTIEVDSAPGRGTTFTLTLPLSRPSGKPQ
                     PAFRQKTEPGRPLKILMVDDDENIREIFQDLLSLDNHRVKTAASGREALEYFAAEPFD
                     VVITDLGMPGMSGWEVTAEVKKLNPQVPVLLVSGWGAQIDEKEAQAKGADLVISKPFQ
                     LNQIRAALLEVSGRATAGRQSAAMSQ"
     gene            complement(8249..>9855)
                     /locus_tag="AC16FU_01195"
     CDS             complement(8249..>9855)
                     /locus_tag="AC16FU_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2993027.1"
                     /translation="ELLLVSEGEFNGDDQLCLLDRDPLARSPIRPGDVFRLELGGRTV
                     GFLWALPSPAVPFETVQNLLKFCQNALAARLGQSLFQTAVVRNRGVGTFLGQWQKTVA
                     SPARLEKTLPQALTVIATIFPCDYLALTLRETGGNSIQRYAVTPAEPGGSGLGSVLLE
                     KGKSLPVPASPSGRVLATGETLVREGFVDAPESDEERFLRALSLLTYVVLPLREKGAV
                     TGTLLLATRQPRARLSLPHRELLSTLSGQLLTQHRLVTVARRRERLLYATVEQAQLVR
                     QATTPDELAARTCTLIAQELPVSFCRIWRVEAPQRRLWSIAFSALRNLGDEVGLAKRC
                     DLADLPWHALACEERKIMRADQSDLESLMPEAELRRALLPGVQKALLVPIISGDRVTG
                     LIAVGDMRSAARRPFTADDEIFLTAVANQMAFAFDRGTVPGRMFPGLPTVKGVSTEAA
                     WPQGTLSPSTFSTLYGAVANPLSAILGSVELLRLKQPELNPQSEKYLQVIERGAWRIK
                     EMVEQPTSGAEAPSVPASDHGTARRP"
BASE COUNT         2050 a         2856 c         2938 g         2011 t
ORIGIN      
        1 ctatttatgc cgtcacagtt aaagtcaaaa cacaaaggat ccggaaggca tattccctga
       61 aagaagcagt ttttcatgat gcagacgtcc tcgaatgaga ccgtcagatc gcagttgaag
      121 tcggtagttc tgaccaagtt gaccgtcaac ttgcaggtat cacccactgc tcggacaatg
      181 tccagtgtaa tcgtgcagtg atctcggcaa ccccctgccc ctatccgaaa ggttacaatt
      241 ccaccggcat tcgaggtatc ttccggataa accaaaggcc acgccgcttc atctggacac
      301 ggacagatgg acgggcaatc ggagaagtta agccagacat tctcctttgg ccagccgctc
      361 accggattgc cgaactggtc gcgcagtctc acctggaacg gctgatcgcc agccggacag
      421 agcaccagac agccaatgat ctcaatcgag tcgcaggtag gtactcccgg ttcaaaacgt
      481 tccaggtggg ttggcgtgcg ggtctggccg tccgccgtcc ctaacgggaa aaggggtgcg
      541 aatccccaca ggacgaaccc gcttaacaag acaagtccga gccatcctac cactgcatgg
      601 attgtcccag tagtcgagtc acgcagagaa ctattcgtcg gatggccatt ggtctgaggg
      661 aggttgcctt tcggcagcgg gtctccaaaa aaagagggca gccctttcgg actgccctcc
      721 ggtgtcactt tttggcttct ccccttcccc ttacgggcac cagttgaacg gacaccccgt
      781 acactgcgtc acgaagacca cgcagttgat gaggcagacg atatccacga tgtcgcagat
      841 gccgtcgcag ttcaaatcgc agcagggtgg gttggcaaag gggcaatcgt tgaagaccac
      901 gcagttgatc agcgccacaa tgtccacaat gtccttgacc ccgtcgccgg ttaagtcacc
      961 cggtatcgag ggacagaaac cgattttcgt cgtcggcacc aataggaaat cgctctctat
     1021 gctggtcaga acgtgcggcc cgaactggtc gaacgtgcct cgcgtctggg taatggtgcg
     1081 cggaggaaaa atgctgtagg cgacgtagtg ggtcgtcagg tcgaagaacg ggtcggcatt
     1141 cttaatacag ggggtgagga agtagaccgg caccagcgtc tgtatctgct cgggtacggg
     1201 atcgaactgg tcttgcaatt gaaccggcct cgtaaacggc accccgtttc taatccggta
     1261 cgccttgtag tggtccaagc cctgcggcgg ggcgtgcggc tgcttctggg tggggacgag
     1321 gagataccgc acggagtcga tctggaccgt ggttgactcg aattgattct cgtaccgcac
     1381 cgtcagcagg gtatccaaac ctctggcccg gtaccagctc aggtgctgcg tggtatcgac
     1441 gatgttaaag gtatcgttcc ttgtcgggtt aatggtaatc ttctgggtcg ggttcgagag
     1501 gtactggatg ctgtcaagcc gcaggaagtc gttctggatg aactgatccc tcacctgggc
     1561 agtcaggaat tgggtcggat tgggcagcag tctccaggtt ttgtagtgat tcttccgcgt
     1621 ggtgggcgga ggattgaatt cgtcgttgtc gcacgcattc gcgcaacccg ggtcagccgg
     1681 gaagtcaatc agcccgtcgc cgtcattgtc aatcccatcg ttgcactggg tcaccgcatc
     1741 gggcgattcg ctgttgtcgc acacatcttt gcacccgcag tcggcgaggt caattaaacc
     1801 gtcgccgtcg ttgtcgattc cgtcattgca ctgggtgacc ggattgggcg cttcggtgtt
     1861 gtcagacgga ttgatgcagc cgcagtcggc cggaaagtca atcagcccgt cgccgtcatt
     1921 atccaaacca tcgctgcact cgggcggtgg gggcggacca aaggacaact tcttcgtcgg
     1981 cacgagcaag agctcgctca ccacgctggt cagaacgtgc attccaaatt ggtcttgggt
     2041 ttgcctctgc tgggtggtgg tgcgctgagg agcaatgcgg taggccacgt agtgggtggt
     2101 gtcgtcgaag aacgggtcgg cattcttcct gcagggcgtg aggaagtaga ccggcaccag
     2161 ctgctgtatt agttcggggt tggcaccgtc gaactggtct tgcagttgaa cctgccgcga
     2221 gaacgccgtc gggtttttaa tccggtacgc tttgtagtgg tcaagcccct ggggcggccc
     2281 atgcggctgt ttttgcgtcg gcaccagaag gtaccgcatc gagtcgatga ccaccgcggt
     2341 ggtctcaaac tgattcccgt acctcacctg gagcaaaagg tcgcggccga tcgctttgta
     2401 ccagctcagg tgctgcgtgg tatcgacgat gttgaaggta tcgttccgag tcgggttaat
     2461 ggtaaccttc cgggtcgggt tcgagaggta ctggatgctg tcgagccgga cggggtcgtt
     2521 ctggatgaac tggtctgata ccgtagcgct caagatggtc gtcgggttgg gcagaaccct
     2581 ccaggtctta tagtgattct tgccggttgg gcgccgcggg cagttgttct ggcaggacaa
     2641 agtcccatca gtccagagca ttagattgcc gcctgaggga ataacagtgc accagtctat
     2701 aaaggtgcct gtcgtctgat cccaatagga gcaacaaggg tcacaagggg cgttgtcaag
     2761 gcaaggaccg accccggggg gaggacagtt gacctggaaa ttcaaacagg caaaataggt
     2821 ggtgtccaca caacaacccg gatttagggg gaatattaat tccatacact gagtgttggg
     2881 gggtatgaca atctgctgga ccgggccggt acaaatcata gcccccggca ccggacaggc
     2941 cgggttggta aaatcggccc ggcgaatacc cggagtgacc agaagcacac aggcagtagc
     3001 attcgcattg gagaagagta tgtgtacgtt cttgacctca aaagggtaag taggacaggc
     3061 ggcaggagga gggcacgttt tggccggatc ctgatattca cacagctccc cccccggaag
     3121 gccactcacg gtgaacgcta cctgtccgct gaagcaggcc atggtgaagg gacagacatc
     3181 ctgtggcccg gttagctgct ttgagggtgc ccaccctgtc ttgcccactg gcgttacctg
     3241 aagagccgtg ttcttcggct ggctggcgtc cgagtttggt ttttgttccg ctcccagagc
     3301 gttacagaca agacctgccg ccaggaggat taccggaccg attaaatctc ttttccgcat
     3361 gtgcctctcc tttccgtttt ctctttcgac tctctttcgc taccgatggt tcggggaaaa
     3421 aataaaaaac acctctaaac gaaggtgctc ttggttaacc tccacttctt tgccccaata
     3481 ggacattttc gcccaccctg gtgccataac tcacacgcct acactccacc cttcacacag
     3541 gccgcgcttc aactcctctt ttaagtcggc acctcccctt cagcaattaa atatagcagg
     3601 tcatattttc ttgtcaagga aaatcttggc tcagcccaaa aaatctttgt gaagaacatc
     3661 acaagcccac caacggcggg ttaagggatg ccggaaaccg tcgataaatc ttaaagaaaa
     3721 ccatagagtt ctgttcggat atctctttcg gaggcaaata ccgtggctac ctgcggacgc
     3781 tgtggtaaag cggaagttga atcgggcgaa caatactgtt ctccttgtag tgcggaattg
     3841 aagaaggacc ctcccgtccc tgctccggcc ggggatgatc cgctgccggt ttcttctccg
     3901 gtaacgatct ggacgggacc ggaatcggcc atgcacgacc ccctgagcac catccataaa
     3961 gagccggagc ggcccgagac caccaaggtt caattggact tttccgggga cgacccgacg
     4021 cctgaccggc aagcaacgcc cactgagccg gcggaacggc tggaattaga atcgcccatc
     4081 gccaaagtcg aagcgagttc gcgcagcatc gggccgcatc cccaggacga gttggcggcg
     4141 caactggtgg aaaatctcga tcagaaaaaa accgggtcac aatcctcccg caagaaaaag
     4201 tcgcccgtca caaatcgcga aagtgacatt tatcacattt ccaactactc cgtaggcacc
     4261 aacgctctgg aagttcagcg gggacgcaag gaaaatgccg aaccgccgct gcccctttcg
     4321 gtcattcgct ccgacgaagc gacgccgcgt tccggaagcg acctggtgat tcaaaccgaa
     4381 ccgccgcgcc gcccgcggcg ggcaccggaa gatccgacct cgggagaagc cggcgaccgg
     4441 catgagcggt atcccggatt aaaggacatc gcctactttt ccggatcgac ggtgcggttc
     4501 accggcggcg tccgcctgca ctcgggcgac gagatcgcca tcggcgaccg cagcttcatc
     4561 ctgcgcgcca agggaacatc gaaactgccg atcgtcctgg ccggggcggt agccggagtt
     4621 ctggcactgg ccgccctcat ctattctttg gtcgggaaac cgaccccgcc ttcggttttg
     4681 tccggcgtag tgtacgatcg agcgtccaag cagctcgtag ggggggtggg aatcaaaata
     4741 gccgaactgg gcaagaccgt cggcaccgac gaactgggct acttccgttt cgatgatctg
     4801 gcccccggaa cgtacacgct ggaagcgctg accccggatg ggtccgccat tcgcaaaaaa
     4861 gtggaaatcc aatccgaaaa gtcgggtttc gtcaccctgg cagtacccgc gcgcaagtct
     4921 cccttacctc ttttgggcag tccggatcaa ccgccgccca atctggcgcg cacggttccg
     4981 gaggccggcc ggactccgcg ggccagtcaa aaagggaatt tgaaaatagc cgtgagcgtt
     5041 cccaacgcta cggtccgtct cgacggaaaa cttctggggg tgggcaatac cacctacgtg
     5101 aatgtcaaaa gcggctcgca ccgggtggcc atttccaaaa tgggctacaa accctggcag
     5161 ggaatcgtcg atatccccca gggcggaacc accaccatca gcgcggcatt agagtcagat
     5221 caggaaggtc ctgccaagtc actctccttt gatgactatc tcaaatcagc tcagaaaaaa
     5281 agtgcggcgg gcgaccacag cggggccgtg gcggattatg agaaagcctt gaacctttcg
     5341 cccaataacc ccctggccct ggctggccgc ggggtcgaac tcgcagagac cggagatcgg
     5401 gccaaagcgc tgaacgattt ccggcaggcc gggagtctct tcttcagcga ccgcaagtac
     5461 ggcctggccg ccgagaattt cagccgggct ttagacattg cgccgtcgga cggtgacttg
     5521 ctgctccgcc gggcgcagag ttatttccgg gccgggcagc tgcgtgagag ccgggaggat
     5581 tacaaagccc tggccaaaca ggcccccaat tccgcgcagg cctacgccgg ctggggactc
     5641 tgcgaatttc agttcggcaa tatgaaagaa gcgatcaagg tttttgagaa agccacgcag
     5701 ctggccccga caaccgcgga gtactacggc tacctcacca tggcccactt cgagagcggg
     5761 aaccgcgaca aggcgcggaa atactatacc aaattcagcg acttcgccaa cctgctcgac
     5821 aagcaggcat ttgaaaagta ccccacctgg cagatggtaa cccgggcgtt cgggtcgtaa
     5881 atccttttta tccgtgagag aagtcggtcg tgccgtccgg aagggcggct tttttgtccg
     5941 cccctcaggt ttcccgctcc gggttacggc ctgcttctcc gtgagggggt cgcttccggc
     6001 gctacgcggg aaagctgctg acagacatta cagttgcgga aaaacatcgg cggaattatt
     6061 gagacatcgc cgcggattgt cgtcctgcgg tcgctctgcc tgagacttcg agaagggctg
     6121 cgcggatttg gttcaactgg aagggcttgg agatgaccag gtcggctcct ttggcttgag
     6181 cttccttttc gtcaatctgc gctccccagc ccgaaaccag tagcaccggc acctgcgggt
     6241 taagcttctt tacctctgcg gtaacttccc agccggacat cccgggcatc cccagatcgg
     6301 tgatcacgac gtcaaacggt tcggcggcaa agtactccag ggcttcccgg ccgctggcgg
     6361 cggttttgac ccggtggttg tccagggaca ggaggtcctg aaagatttcc cggatatttt
     6421 cgtcatcgtc caccatcaaa atcttgaggg ggcgtcccgg ctccgtcttt tggcgaaaag
     6481 ccggttgcgg tttcccggaa ggccggctga gtggcagcgt gagggtgaat gtcgtgccgc
     6541 ggcccggcgc cgaatcgact tcaatcgtcc ctttgtgccg ggaaatgatg ccgtaggaaa
     6601 ccgacagtcc cagaccggtg ctcttgactc ccttggtagt gaaaaaagga aagaaaattt
     6661 tcgatttgat ctcttcaggt attccctgcc ccgtatcggt gacgatgact gaaacccgtt
     6721 caccgtccaa gcgggaagtg aaagtgaggg ttccccccgc cggcatggcg tccacggcgt
     6781 tgctgatcaa attggtgaat acttccacca gttcggcggc gttgccctga accggcggca
     6841 ccggctccag ccggttttcg atcgcaatga atccgccgct ctcctgcacc tgatcgcgcc
     6901 agcggggctt ggtcacctca atcgcttcgc ggatgacctg acccaggtca acggcggtga
     6961 aggcggcgtc ggttttcacc cgggtgaact cctgtaagcg ggcgaccgtc cgcgcccccg
     7021 ccagggtgag ttcttgaatt tgctccagac cgagacgggt cttttcatcc tgcgcctgcg
     7081 tcaggagcag ttgcacccgc cccaggacgg caccgagcat gttgttaaaa tcgtgggcta
     7141 ctcctccggc catctctccc agtgcgcgca gcttttcggt ctgcaccatc tgctcctggg
     7201 ccgtgaacaa ctgctggtag gcctgctcca gatttttgta cagccgggcc atttcgagaa
     7261 catcggcggc ctggcgggcg aaaatggtca agagttccaa atcctcggac tggaactccc
     7321 gccctcccag agcggtcagg gtaatgacgc cgatcacatc gccggcgtac aggagcggca
     7381 cggaaatgag cgattcgacc tccaagggcg tatcgggaac ctgcaccgcc gtttcgtcct
     7441 tttccgcgtg attgaggatc tggccgacgc cagtctgggc cacctttccc gagagtcctt
     7501 cccccagggg tatcgtgaag gccatgatct gctcgcggta gggttcccgg ttggtcatca
     7561 ggggctggag cagtccggct tctttgtcgc agaggtaaat ggtgccgccg tcggcgcgcg
     7621 ccagcttggt cgccagggcc aaaagtttct ccagcaccac ctggtgattg agggaggaat
     7681 acatcgtggt ggcgagttcg gagagtgcgg tgaggcgctg attacctttt tccagttccc
     7741 gccccaagcg taaccggcag agttccgccc gggagaaatc gcccaaaagg cgcgccagca
     7801 ccaaatcggt ctcaccgaat tgatggggcg cccggtgcca gatggcggcc agagccgcct
     7861 gggtgtagcc gttatccgtt acgggaacgg tcaagaccgc ggtgacctga aaaatctgct
     7921 gaaggtcgct ggagagttca cccgttcccc ccgtgcccca ggtgattggt tcttgattgg
     7981 cggctcgtcg gacggaaaag gtttcggcag cgacgatttc ccaaaaccgt tccggtaact
     8041 ccggcggcgt ctgagcgcag atgatccgga acccatcagc cggagtctcc cgccagaaga
     8101 atccaaaacc ggagcagccc aaaaccgtcg aaagccgttc tcccaagagg ttgccaatgg
     8161 cggaaaggtc cgccgtcgac attctctgag taccgtttga ctgcatccgt gttacccgaa
     8221 ctgaagccgc agcaaccgaa agcaccgttt acggtcgccg agcggtcccg tggtctgagg
     8281 cgggcaccga cggtgcttcc gcgccggaag ttggctgctc caccatttcc ttgatgcgcc
     8341 aggcacctcg ttcaatgacc tgcaggtact tttcactctg cggattgagt tccggttgtt
     8401 ttaggcgcag gagttccacc gaaccgagaa tggccgagag cgggttggcc accgctccgt
     8461 acagggtcga aaaggtcgac ggggatagtg ttccctgggg ccaagccgcc tctgtcgaga
     8521 cccccttgac cgttggtaat ccgggaaaca tccttcccgg gacggttccc cggtcaaagg
     8581 cgaaggccat ttgattcgcc accgccgtca aaaagatttc gtcgtcggcg gtgaagggac
     8641 ggcgggccgc tgagcgcata tcgccgacgg caatcaggcc ggtgacgcgg tcacccgaaa
     8701 taatggggac gagcagggct ttctgtacgc ccggcaaaag tgcccgccgg agctccgctt
     8761 cgggcatcaa actttccaga tcgctctgat cggcccgcat gattttcctt tcttcacatg
     8821 ccagggcgtg ccaggggagg tcggccaagt cacacctttt ggccaaaccg acttcatctc
     8881 ccagattgcg cagcgcggag aaagcaatgc tccagagccg tctttgcggt gcctccaccc
     8941 gccagatccg gcagaaggag accggcagtt cttgcgcaat caacgtgcag gtgcgggcgg
     9001 cgagttcgtc gggggtcgtg gcttggcgta ccaactgggc ctgttctacg gtggcgtaca
     9061 gaagcctttc ccggcgccgt gccaccgtca ccaaacggtg ctgggtgagt aactgaccgc
     9121 tcagggtgct gagcagctcc cggtgtggca gggagagccg cgcccgcggc tgacgggttg
     9181 ccagaaggag tgtgccggtg accgcacctt tttcgcgcag gggcagtacg acgtaggtga
     9241 gcaagctcag agcgcgcagg aaccgctctt cgtccgattc cggtgcatcg acgaaaccct
     9301 cccgcaccaa ggtttcgccg gtggccaaaa cccggccgct gggcgatgcc ggtacgggga
     9361 gacttttccc tttctcaagg agcacggaac ccaacccgga ccccccgggc tccgcaggag
     9421 tcaccgcgta gcgctggata gaattgccgc ccgtttcccg caaggtcagg gcgagatagt
     9481 cgcaggggaa aatggtggcg atgaccgtta acgcctgggg caaggttttc tctaagcgag
     9541 ccggagaagc gactgttttt tgccactgcc cgaggaacgt tcctactccc cggttgcgga
     9601 cgactgccgt ctggaaaagt gactgcccca gccgggcggc cagggcattc tggcagaatt
     9661 tgagtagatt ttgaaccgtt tcgaagggaa ctgcgggact ggggagagcc cagaggaatc
     9721 caactgttcg gccgccgagc tcgagccgaa acacgtcgcc gggtcggatc ggcgagcggg
     9781 cgagggggtc tcgatccaaa agacagagtt ggtcatctcc gttgaattct ccttcgctca
     9841 ctaacaggag ttccc
//
LOCUS       DBNGZA010000059         4858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1176896, whole genome shotgun sequence.
ACCESSION   DBNGZA010000059 DBNGZA010000000
VERSION     DBNGZA010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1176896"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC16FU_01200"
     CDS             complement(<1..285)
                     /locus_tag="AC16FU_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993028.1"
                     /translation="MKKSKAIWAVTGLVVLGGLWLWFAGCGGSAKGGIDTKHMVYGSP
                     ESKMSMTPASGPLYDGAALSIAPEVKPVPNTNEHHVRIDVTHSEIVVNDTV"
     gene            complement(888..1676)
                     /locus_tag="AC16FU_01205"
     CDS             complement(888..1676)
                     /locus_tag="AC16FU_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2993029.1"
                     /translation="MKRATAHFTVITASAVILAGIGFSWARPDSTGRGSLTGEAGRGK
                     LVYERYCISCHGAEGDGQGEFAEWISPKPRDFRQGMFKWRSTPSGSLPADADLEKTLE
                     NGTYGTHMPAWYVLGHREHREVIAYLKTFSPRWTKESAQPALVLPPEPPNDAASVERG
                     HAVYVSTGCDRCHGDRGQGDGPSARGLKDDWGYPIVPYNLTMGRIKCGETAADIYRVL
                     FTGLSGTPMPSFEKSLTGDQAWDLAHYILSLGHRAQQRIAKKGP"
     gene            complement(1686..3005)
                     /locus_tag="AC16FU_01210"
     CDS             complement(1686..3005)
                     /locus_tag="AC16FU_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993030.1"
                     /translation="MGRSLFKLLTGVTVAALLFILPLTQVSSIPSFARKTGLRCTSCH
                     ESWPVLNDFGRLYRDKGYQLRLGQDDPTTTTPGYWPAAIRATPHYEFNTLTNQETDSG
                     KTDFKTGGFADIGMDLLTGGTLSENAAFQVVAAGFSPDESPTLESYWVYLPKLFFKSD
                     WFNLRLGKYELDLPLSGHRSINLTNPYLVYGYHPEPDDNAAAAFSLAENQRGVEIVGT
                     DRRSFTRYVLSVFNANESPGSRTVFDSPSGYAHVQKFWQTASRAAPEIALGVFGAYAN
                     YPTTSLTSAGEPIPGQGGNLQGSLRYGGEAQVWFGPLVAPLHLVLVYAHGKDKRALFT
                     GADQDGIWNGGFAQLIWVPPAERLHWGLFARYDLIRNSRQPLAALPKDLNDQDQFTLG
                     LKYTISYSNRTEYALHAEYSTDRIRKTAFDGSDIRAGTAFIGIDFAY"
     gene            complement(3202..3951)
                     /locus_tag="AC16FU_01215"
     CDS             complement(3202..3951)
                     /locus_tag="AC16FU_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR2993031.1"
                     /translation="MKRILTFTLMAFSIAATAWGGYDAIQVENGGWITGKISYAGGPP
                     VAKKIEPTTDKAACGKHGPIFSEELTVSPDSGLQNAVVSIANITAGRSVFSMAPPVLE
                     QNGCTFRPHVLVVPVGKPLTVRNSDGLLHNIHTHSLKNPPVNIAQAAAVPEITLGPYT
                     VPELVKVGCDIHGWMSAWFWVTNNPYAVVTGANGSYKIADLPPGKYVVEVWHETLGKI
                     SSEVTVAAGKETRLNLTFPAARPQVQAKKSD"
     gene            4435..>4858
                     /locus_tag="AC16FU_01220"
     CDS             4435..>4858
                     /locus_tag="AC16FU_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HIR2993032.1"
                     /translation="MKKGQFWALGLTGFFILASLWSLFPTFRFWTLPVEKRTEQPELE
                     KKAMKLGLDLRGGMHIVMEVDKSKLNEAEKKDAVDRALEIIRNRIDRFGVTEPQIQKS
                     GQDRIVVELPGVQEVERAKNLLGQTAQLEFKLLETTENT"
BASE COUNT          940 a         1487 c         1375 g         1056 t
ORIGIN      
        1 gaccgtgtcg ttcaccacga tttcggagtg ggtgacatca attctgacgt ggtgctcgtt
       61 ggtgttcggc accggcttca cttccggggc aatggagagc gccgccccgt cgtacagcgg
      121 tcccgacgcc ggcgtcatcg acattttcga ttccggcgag ccgtagacca tatgcttggt
      181 gtcaatcccg cccttggcgc tcccgccgca cccggcgaac cagagccaaa gcccccccag
      241 tacgaccaac cccgtcactg cccagattgc cttactcttt ttcacttcct cctcctttgt
      301 tgctcccgcc tgttatcgaa cgccgacgtg aattgattat actattttgc tttcatccct
      361 gaataccgga aaccgatcac ctcctctatt ttccttgccg tgtgtctcgt gtggatcccc
      421 ttttttgaac tttttgcgcc cgcatgtgaa atatttcaca ttaccggaca ttcgtccacc
      481 tctttcccat ccatacgcta cctatttact cttgtatgct attatcggat actataatcc
      541 gtttgtcaag tgctttttcg ccccgggagc gcccaccctc ttcccttccc ataggtcagg
      601 catctcgcga gagtcgcgcc gacctgcctg accgctcacg tggccccgga aaggccgact
      661 tcaacctgtg gacgccaact atttccctat cacccaaaga gacaatgagc tccggctgcg
      721 gactgatcgg gcatagccca agtagccgcc gcagccggct ttaccgtagt tggtgcaggt
      781 ggtcgcgtta gccccggctc aatttcaatg aaaaagcggc ttggctcaaa gaagtcccgg
      841 gatgtgcaga ctcgttttct tgcggcaccc tgctcgtaac cccggcccta tgggcctttt
      901 ttcgcgatgc gctgctgcgc ccggtggccg aggctcaaaa tgtagtgggc gagatcccag
      961 gcctggtctc cggtgagcga tttctcgaag gagggcatgg gagtcccgct gaggccggtg
     1021 aaaaggacgc ggtagatgtc ggcggccgtt tcgccgcact tgatgcggcc catcgtgagg
     1081 ttgtacggca cgatcgggta tccccagtcg tctttcaaac cgcgggccga cggcccgtcg
     1141 ccctggcccc ggtcgccgtg gcagcggtca cagccggtgc tgacgtaaac tgcgtgcccg
     1201 cgctcgacgg aggcggcatc gttgggcggt tcgggaggca ggacgagggc gggctgggcc
     1261 gactccttcg tccagcgggg cgagaaggtt ttgaggtagg caatgacttc ccggtgctcc
     1321 cggtggccga gcacgtacca ggctggcatg tgcgtgccgt aggttccatt ctcgagagtt
     1381 ttttcaagat ccgcatcggc gggcaaagat cccgagggcg tcgagcgcca cttgaacatc
     1441 ccctggcgga aatcccgcgg cttgggcgag atccattcgg caaactcacc ttgcccgtcg
     1501 ccttcggcac cgtgacagga gatgcagtat ctctcgtaga ccagttttcc ccgcccggcc
     1561 tcccccgtca ggctgccccg gccggtgcta tccggccggg cccaggagaa gccaataccc
     1621 gccagaatga cggcgctggc ggttatgacc gtaaagtgag cagtcgctct tttcatctcg
     1681 tcctcctaat aggcgaagtc aatgccgatg aaagccgtcc ccgcccgaat atcggatcca
     1741 tcgaaggcgg tcttccggat ccggtcggtg gaatactccg catgcagcgc atactccgtg
     1801 cgattgctgt agctgatcgt gtacttgagg ccgagcgtga actgatcctg gtcattcaaa
     1861 tccttcggga gcgctgccag gggttgccgg gaattccgga tcagatcgta gcgcgcaaag
     1921 agcccccagt gcagccgctc ggctggcgga acccagatca gctgcgcaaa gcctccattc
     1981 cagatgccgt cctgatcggc ccccgtgaaa agggcgcgtt tatccttgcc gtgggcgtag
     2041 accaagacca gatgcaaagg cgcaaccaaa ggcccgaacc agacctgggc ttcccccccg
     2101 tagcgcagcg acccctgcaa gtttcccccc tggccgggaa tcggctcccc ggccgacgtg
     2161 agggaggtgg tggggtagtt cgcataggcg ccgaagaccc ccagggcaat ttccggcgca
     2221 gcgcgggagg ccgtctgcca gaatttttga acatgcgcat agcccgaagg ggaatcaaag
     2281 acagtgcggc tcccgggaga ctcgttcgcg ttgaaaaccg acaggacata ccgggtaaaa
     2341 gaacggcggt cggtgcccac aatttctacg ccgcgttgat tctccgccag gctgaaagcg
     2401 gctgcggcgt tgtcgtccgg ctccgggtga tacccgtaga cgaggtaggg attcgtcagg
     2461 ttgattgatc gatggcccga aagcggcagg tcgagttcgt actttcccaa ccgcaggtta
     2521 aaccagtccg acttgaagaa caatttcggc agatagaccc agtacgactc caaagtcggg
     2581 ctctcgtcgg gagaaaagcc cgccgcgacg acctgaaagg cggcgttttc gctcaaagtg
     2641 ccgccggtca acaggtccat cccaatgtcg gcgaacccgc ccgttttgaa atcggttttt
     2701 cccgaatcgg tttcctggtt cgtcagggtg ttgaattcgt aatgcggggt cgcccggatc
     2761 gccgcgggcc aatagcccgg cgttgtcgtc gtcggatcgt cctggccgag ccgcagctgg
     2821 tagcctttgt cgcgatagag ccggccgaaa tcgttgagca ccggccagga ttcgtggcag
     2881 ctggtgcagc gcagtccggt cttccgcgcg aacgaaggaa tggaagaaac ctgggtgagc
     2941 ggaaggatga aaagcagtgc tgcgacagta acgccggtga gaagtttgaa cagcgaacgg
     3001 cccatgaagc acctcctgtc ttgagccctg ggcttgggca ccgccggcga cgtgatcatg
     3061 tcgggcggct gcggtgggcg aaccgaacgt taagcacaac ggttgcggct gccacaggca
     3121 gagctgcccc gcctgccagc cgcattgctt cagcgagact tttcatccgt gactcttttc
     3181 gggcggctaa gggacgaacc gttagtcact ttttttcgcc tgcacctggg gccgggctgc
     3241 cggaaaagtc agattcaaac gggtttcctt tccggccgcg acggtgacct cgcttgaaat
     3301 ttttcccaac gtctcgtgcc agacttcgac cacgtatttt cccggcggca gatcggctat
     3361 tttatagctg ccattcgccc cggttaccac ggcataggga ttgttggtca cccagaacca
     3421 ggccgacatc cacccatgga tatcgcagcc caccttcacc agttccggta cggtgtaggg
     3481 gcctaaggtg atctccggca ccgcggccgc ctgggcgatg ttaaccggcg ggttcttgag
     3541 cgagtgggtg tgaatgttgt gtaaaagtcc gtcgctgttg cgcacggtca gaggtttgcc
     3601 gaccgggacc accagaacat gcgggcggaa ggtgcatcca ttttgctcta agaccggcgg
     3661 cgccatactg aaaaccgacc ggccggcggt tatgttggca attgaaacca cggcgttctg
     3721 caggccgctg tccggggaga ccgtcagttc ctcagagaaa atcggcccgt gcttgccgca
     3781 ggccgcctta tcggtcgtgg gctcgatctt tttggccacc ggcgggccgc cggcatagct
     3841 gattttcccg gttatccagc cgccattttc cacctgaata gcgtcgtagc cgccccacgc
     3901 cgttgcggcg atagagaagg ccatcaaagt gaaggttaaa atccgcttca tcctttcctc
     3961 cttaattaag ggttatttga ccgaaccgct gaacaaaaaa taatctctta gctatttctc
     4021 ttgttatttt tttccacaac caaaccttcg cgtacaccct tactccttcc tcaccgatac
     4081 gctacccgac aactcttgtt tgctattatc ggataccatt atccgcttgt caagtacttt
     4141 ttactccaga cccttttttt atctcgtccc tgggtcaggc atctcgctag agtcgtgccg
     4201 acctgcctga ccgctgacgc ggtcccgaaa aggccgcctt caacctgtgg acgccgcctt
     4261 tctttcccta tcacccacca ccttccacct acttgtggtg agtcccgccc atacgggccg
     4321 tatggccaaa ggcgggatcg aaccatcacc tgacttttga cttttatccc cgcccgctat
     4381 atataccccg ttcccccggt gcggttttcg caacccgggg ttggacactg accgatgaaa
     4441 aaaggacaat tctgggccct gggcctcacc ggctttttca ttctcgcttc cctctggagt
     4501 ctttttccca cgttccgctt ctggaccctc cccgtcgaga agcgcaccga gcagccggag
     4561 ttggaaaaga aagcgatgaa gctgggcctg gatctccgcg gcggcatgca cattgtcatg
     4621 gaggtcgata agtccaaatt aaacgaggcc gaaaaaaagg acgccgttga ccgggcgctg
     4681 gagatcatcc gcaaccggat cgaccgcttc ggcgtcaccg agccgcagat tcaaaagtcg
     4741 ggccaggacc ggatcgtggt cgaactcccc ggggtgcagg aagtggagcg ggccaagaac
     4801 cttttgggcc agactgcgca attagaattc aaacttttag aaactactga aaatacga
//
LOCUS       DBNGZA010000060         4423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1921261, whole genome shotgun sequence.
ACCESSION   DBNGZA010000060 DBNGZA010000000
VERSION     DBNGZA010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1921261"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="AC16FU_01225"
     CDS             complement(<1..1787)
                     /locus_tag="AC16FU_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR2993033.1"
                     /translation="MKGLKPEDINIILATDCGSTTTKAILIEKRGEEYRLIVRGEAPT
                     TVEAPFEDVTMGVLNAVAEVEELSGRKLIDEKGQIISPAKGNVGTDIYVSTSSAGGGL
                     QMMVAGVVRSMTAESAERAALGAGAIVMNVIASNDKRLPHQQIERIRRLRPDMILLSG
                     GIDGGTTTHVVEIAELVSAADPRPRLGHGYKLPVIFAGNKEAREPVKQTLAGKTDLTV
                     VDNLRPVLEWENLLPAREKIHDLFMEHVMAQAPGYRKLMSWTDAPIMPTPGAVGLIMK
                     RIADEEGIEVVGVDIGGATTDIFSVFQGVFNRTVSANLGMSYSISNVFAEATLPNVMR
                     WVPFVMDERDLRNRVKNKMIRPTTIPQMLEELIFEQAIAKEALRLAFIQHKSFATTLK
                     GIQQQRTIAEAFEQGASGLTLVNMMTLNMLIGSGGVLSHAPRRQQAALMLIDAFLPEG
                     VTRLSVDSIFMMQQLGVLTEVHSRAALEVFRKDCLIHLGTCVAPVGQDKEGREILDFR
                     FEMPDGTATEGKLKFGEMVRIPLGFDDKEVTLKAKAVLNPTRNFDVGAGRGAKLDTTV
                     SGGVAGIILDGRGRPFNLPTDPQTRVRKLT"
     gene            complement(1941..2348)
                     /locus_tag="AC16FU_01230"
     CDS             complement(1941..2348)
                     /locus_tag="AC16FU_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993034.1"
                     /translation="MRKALLVLAGLLLAASAAQAQQRRTISPVGTGVKGNPTLEFMIG
                     PMFGSGTLRGFDDPRTFEQDLNYTGFFLNSGLTLPIQPRVALLFKGDFNWLRSKADFP
                     VVVSPVGAVQDIFEIQRTAGVVKLGFGVRIELK"
     gene            complement(2418..2840)
                     /locus_tag="AC16FU_01235"
     CDS             complement(2418..2840)
                     /locus_tag="AC16FU_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993035.1"
                     /translation="MDFRHAFSVSGSQPKGAPLTPEQEAILDKIARKVVHWKMTVPAI
                     MFLETSKPLSFLGSQTMVFFEPVIQSIFSFKDYDTVRKLLEERENVERLLLKIEQFDA
                     EALQAEKTAKDQKPSAQVTFRKRVARLFGRNRPPTPLR"
     gene            complement(2848..3303)
                     /locus_tag="AC16FU_01240"
     CDS             complement(2848..3303)
                     /locus_tag="AC16FU_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993036.1"
                     /translation="MSEVALAWTTFPFTRNWKVSGAVILFLLVVFFFVYQWIHSILFL
                     TICMVLVIGSLAGFFFPTEFQLTETGLVVRSLFGTQERPWTSFRSFYPDRNGVLLSPF
                     PRPSRLENFRGIYVRFDNNREQVLAFVQQRLGCGEITAPRSDTLASHTG"
     gene            complement(3311..3532)
                     /locus_tag="AC16FU_01245"
     CDS             complement(3311..3532)
                     /locus_tag="AC16FU_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993037.1"
                     /translation="MSNDPNRRFALKLIIGGKEREVTFEELALSNNLAQEALVRILIQ
                     KKIFTPEELLKAMEEVKKERYRSAGEAGG"
BASE COUNT          941 a         1318 c         1196 g          968 t
ORIGIN      
        1 gtcagtttcc gcacccgggt ctgcggatcg gtggggaggt tgaacggcct gccccggcca
       61 tcgaggataa taccggccac cccgccggag acagtcgtgt ctaacttcgc gcctctgcct
      121 gcgccaacat caaaattccg cgttggattt aagaccgctt tggccttcag cgtcacttct
      181 ttgtcgtcaa atcccaaggg gatgcgaacc atctcgccga attttaattt cccttcggtg
      241 gccgtgccat ccggcatttc gaatcggaaa tcgagaatct cccgcccttc tttatcctgc
      301 cccaccggcg ccacgcaggt gcccaagtga atcaggcagt ctttgcggaa gacctccaga
      361 gccgcccggg aatgcacttc ggtcaaaacc cccaactgct gcatcatgaa aatcgagtcg
      421 accgaaaggc gcgtcacccc ttcgggcaga aaggcgtcga tgagcatcaa ggccgcctgc
      481 tgccgccggg gcgcgtggga gagcaccccg ccggagccga tcagcatgtt caaggtcatc
      541 atgttcacca gggtcagacc cgaagcccct tgctcgaacg cctccgcaat cgtcctttgc
      601 tgctggatgc ctttgagcgt cgtggcgaac gacttgtgct ggatgaaggc cagccgcagc
      661 gcttctttgg cgatggcctg ttcgaaaatc aactcctcca gcatctgcgg gatggtggtg
      721 ggccggatca ttttgttttt gacccggttg cgcaggtcgc gctcatccat cacgaacggc
      781 acccagcgca tcacgttggg caaggtcgcc tcggcaaaga cgttggaaat cgagtacgac
      841 attccgaggt tggccgaaac cgtccggtta aacacaccct ggaaaacaga gaagatgtcg
      901 gtggtcgccc cgccgatatc gacgccgacg acctcgatcc cctcttcgtc cgcgatccgc
      961 ttcatgatca aaccgaccgc acccggcgtc ggcataatcg gtgcgtcggt ccaggacatc
     1021 agtttccggt accccggcgc ctgcgccatt acgtgctcca taaagagatc gtggattttc
     1081 tcccgcgccg ggaggagatt ctcccattcc agcaccggcc gcaggttatc gaccacggtc
     1141 aggtcggtct tgccagccag tgtttgcttg accggctccc gcgcctcctt gttcccggca
     1201 aaaatcaccg gcagtttata cccgtgccct aaacgcgggc gtgggtcagc cgcgctgacc
     1261 aattccgcga tttcaacaac gtgcgtggtg gtccccccgt caatcccgcc ggagaggaga
     1321 atcatatctg gccgcagacg gcggatgcgt tcgatttgct ggtgcggcag gcgcttgtcg
     1381 ttggaagcga tcacgttcat tacaatcgcc ccggcgccca gggccgcccg ctccgccgac
     1441 tccgcggtca tcgagcgcac caccccggcc accatcatct gcaagccgcc gccggcggag
     1501 gaggtggaga cgtaaatatc ggtgccgaca ttccccttcg ccggagagat gatctgcccc
     1561 ttttcgtcga tcagcttccg tccggagagt tcttccactt cggccacggc attgagcacc
     1621 cccatggtca cgtcctcgaa aggcgcctcg acggtggtcg gcgcttcgcc ccggacgatg
     1681 agccggtact cttctccgcg cttttcgatc aggattgcct tggtggtggt cgagccgcag
     1741 tcggtggcta agatgatgtt aatgtcttcc ggtttgagac ctttcattgt ttcctctatc
     1801 ttgaagtaga gtcctgcaaa aacaaaatca cccttcggca cccgccgggg agttgcggca
     1861 gaatatacaa aaaaacaggc aacccaccac cccttttggg gatggtcggt ggcctgcttt
     1921 tgatctgacg cctgtggcgc ttacttcagc tcgatccgca cgccgaagcc gagtttgacg
     1981 acgccggccg tgcgctggat ctcaaatatg tcttggacgg ccccgaccgg actgacgacc
     2041 acggggaaat cggctttcga gcgcagccag ttgaaatcac ccttaaacag gagcgccacc
     2101 cgtggctgaa tgggaagtgt cagcccgctg ttgaggaaga agccagtgta attgagatcc
     2161 tgttcaaacg tgcgcgggtc atcaaacccg cgcagcgtcc cggaaccgaa cattggtcca
     2221 atcataaact ccagcgtggg gttgcctttg acgccggtac cgaccgggct gatcgttcgc
     2281 cgctgctgcg cctgcgctgc gcttgccgcg agcagaagcc ctgccaggac gagcaatgcc
     2341 ttccgcatgg tgcctccttt gggtttaagg ttggtttccg tacctacccg tacttgtcgg
     2401 cgtatctgga gcttcgctta cctaagggga gtgggcggtc ggttgcgccc gaacagccgc
     2461 gcaacgcgtt ttcggaacgt cacctgagcc gacggcttct ggtcctttgc cgttttctcg
     2521 gcctgcagcg cctccgcgtc gaactgttca attttcaaaa gcaaccgttc gacgttttcc
     2581 cgctcttcca agagtttgcg caccgtgtca tagtctttga aggagaagat cgattgaatg
     2641 accggctcaa aaaaaaccat ggtctggctg cccagaaagg agaggggctt ggaggtttcc
     2701 agaaacatga tcgccgggac cgtcatcttc cagtggacca ccttacgggc gattttgtcc
     2761 aatatcgctt cctgctccgg cgtgagcggg gctcctttgg gttgggaacc ggataccgaa
     2821 aaagcgtggc ggaagtccac ggtgacttta cccggtgtgc gacgcgagcg tatcggaacg
     2881 gggtgctgtg atctccccgc accccaaccg ctgttgtacg aacgccagca cctgctcccg
     2941 attgttgtca aaccgcacgt aaatcccccg gaagttctcc aaacgcgacg ggcgcgggaa
     3001 aggcgaaagc agaacgccat tccgatccgg gtaaaaactg cggaaggacg tccagggccg
     3061 ctcctgcgtg ccaaaaagcg accgcacgac cagcccggtc tcggtcagct ggaactcggt
     3121 ggggaagaaa aacccggcca gagagccgat caccagcacc atgcagatgg tgaggaaaag
     3181 gattgaatga atccactggt agacgaagaa aaaaaccacc aaaaggaaaa ggataaccgc
     3241 ccccgaaacc ttccagttcc gggtgaaggg gaaagtggtc caagccagcg caacctcgga
     3301 catgcagacg ctatcctccc gcttctccgg cactccggta gcgctctttt ttcacttcct
     3361 ccatcgcttt caagagttct tcaggggtaa aaatcttctt ttgtatcaaa atccgcacca
     3421 gcgcctcctg ggctaagttg ttggagaggg ccagttcttc aaaggtcacc tcgcgctctt
     3481 tcccgccgat gatgagtttc agggcgaacc tgcggttagg atcattcgac acgtgaactc
     3541 caaaagtgtg aaggttctaa ggtcttactt tttcccggta ttgtttcctt cggaccaaaa
     3601 gtggaacatt ggttcaattc caatctatgt cttgacttag acttattctc cattggtggg
     3661 tgtaggtttt ccgccacgta caacgatttt cggatgaagg aaaacggatt tgtcttgaag
     3721 tatttttgcc gggtttctga ccccaaaagt gtgagtacgc cctcctcccg cctcacttgc
     3781 cgtacttcct tcgtacttcc aaccggcctt gtccagaaaa actacatcca ctcgccagat
     3841 gacgacacgt ctacccggcg agacgctact tgttggatca acgagataac gccagacgat
     3901 ataccacccc gggtggtcgt agtgggattg gacggcccat acgcgcttgc tccctttaga
     3961 ttctaaggac aatctaggct gcggcggcaa ccgactctta cggtgcctca aatccggaaa
     4021 acgctgttga gcaagatctt gatgtctgtg tttttcgtaa ggactaaagg catctagcgc
     4081 tttgacaccg aagatactaa cgagggcact gaaaacgttg gcttggtcgt aataaacttc
     4141 gacgaactcg tcgagctctc tctcaatgta ggaaatggcc cgtcttatta ttcccaccgt
     4201 gagatcagga ggtaaacgaa caccggaatc ccatcctctg gaatccctca tttctttgga
     4261 ctctcaacgg tacgggtaac tctttggctg atacgagcga aattaacaaa acgaccagac
     4321 atgttcccat taagggacaa tgcggcgagc cgtgggatgg cacgcctagc catgcgaaaa
     4381 tagtgaggat ccttctcgat gccaatactc gcatatccta cag
//
LOCUS       DBNGZA010000061         1514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2874720, whole genome shotgun sequence.
ACCESSION   DBNGZA010000061 DBNGZA010000000
VERSION     DBNGZA010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2874720"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16FU_01250"
     CDS             complement(<1..868)
                     /locus_tag="AC16FU_01250"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR2993038.1"
                     /translation="MKRLTPLAIALLWACSPSPETSQAQAPKSPTPAPVEAPVKVETF
                     ASGLEHPWGLAFLPDGRMLVTERPGRLRIVSRDGKLSPPLGGVSRVFAQGQGGLLDVC
                     LDPKFAENHLVYLSYAEPGENGTAGTAVARGRLGRNRLDSVRVIYRQTPKVGGIPNHF
                     GSRLVFAPDGKLFITQGDRYSQRNRVQDLSNDFGKIVRINPDGSIPKDNPFVGRKNAR
                     PEIWSYGHRNVQGAALNPETGQLWTLEHGARGGDELNHPEAGKNYGWPVITYGVDYSG
                     EKIGEGTAKPGME"
     gene            complement(1059..>1514)
                     /locus_tag="AC16FU_01255"
     CDS             complement(1059..>1514)
                     /locus_tag="AC16FU_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HIR2993039.1"
                     /translation="LANHDVMLWAVSRAPLAKLQAYKRRMGWMFPWASSFGSDFNFDF
                     SVGFTEEQQREGGIEYNYRREAGRQFRKGEGPVEFAAMTGTDPATYIRERPGMSAFAL
                     EDSVVYHTYSTYARGLDGLWGMYQWLDRAPKGRNETGLWWRRHDEYDKR"
BASE COUNT          280 a          489 c          440 g          305 t
ORIGIN      
        1 gttccattcc cggcttggcc gttccctcgc cgattttctc tccagagtaa tccacaccgt
       61 aggtgataac cggccagccg tagttcttac ccgcttccgg atgatttaac tcatccccgc
      121 cgcgcgcccc gtgttccaac gtccagagct gcccggtttc gggattaagc gcggccccct
      181 gcacgttgcg gtggccgtac gaccagattt ccggccttgc attttttcta ccgacgaaag
      241 gattgtcctt ggggatgctg ccgtccgggt tgatgcgcac gattttgccg aagtcgttgg
      301 acaaatcctg aacgcggttg cgctggctgt agcgatcccc ttgcgtgatg aacagctttc
      361 catccggcgc aaaaacgagc cgggagccga agtggttggg gatgccgccg acctttggtg
      421 tttgccggta gattacacgg acgctgtcga gacgattccg accaagccgt ccccgcgcca
      481 ccgccgtccc ggccgttccg ttctcccccg gttcggcgta cgagagataa accagatggt
      541 tttccgcaaa ttttgggtcg aggcaaacat caagcaatcc cccctgcccc tgcgcgaaga
      601 cccgtgaaac accccccaag ggcggagaaa gtttgccatc ccggctaacg atgcgcaacc
      661 gccccggccg ctcggtgacg agcatcctcc cgtccggcag aaaggccaac ccccagggat
      721 gttcgagccc ggaagcaaag gtttcaactt tgactggcgc ctccactgga gcgggcgtcg
      781 gcgatttcgg cgcctgcgcc tgtgaagttt cgggggaggg actgcaggcc caaagtaacg
      841 cgatggcgag cggggttaac cgtttcatct cttcctttca ggcgaaagat ccgctgtcaa
      901 acaaaagaat taacccggct ttaacccgcc ggtcaagcga agtattttcc ctcccctcaa
      961 ggggaggaat ttagaccttc gccgtagacg gaggtcgaag ggtggggtca aaaattaccc
     1021 gcctcttaaa agttgcgtca taacaaccac gacgccgttc agcgcttgtc gtactcgtcg
     1081 tggcggcgcc accagaggcc cgtctcgttg cgccccttgg gggcgcggtc gagccactgg
     1141 tacatccccc agaggccgtc cagtccgcgc gcgtaggtgg aataggtgtg atagacgacg
     1201 ctgtcctcga gcgcaaacgc gctcatgccc ggccgctcgc gaatgtacgt ggccgggtcg
     1261 gttcccgtca tggcggcaaa ctcgacaggc ccctcgccct tgcggaactg cctccccgcc
     1321 tcgcgccggt agttgtattc gatgcctccc tcgcgctgtt gctcctcggt gaacccgacg
     1381 ctgaagtcga agttgaagtc gctgccgaat gaggaggccc aggggaacat ccaccccatc
     1441 cgccgcttgt acgcctgcag tttcgcgagt ggcgcgcgcg agaccgccca gagcatcacg
     1501 tcatggttgg ccag
//
LOCUS       DBNGZA010000062         4899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1708271, whole genome shotgun sequence.
ACCESSION   DBNGZA010000062 DBNGZA010000000
VERSION     DBNGZA010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1708271"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..947)
                     /locus_tag="AC16FU_01260"
     CDS             complement(102..947)
                     /locus_tag="AC16FU_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993040.1"
                     /translation="MNRKYLGFAVLLLLFSSSASAGPFVDSLIIASKTFTGSIGVIEV
                     AISNDSAMNGWVMSFLISSTTGGAFGKVDSVQALPSLNPLGITLNLTKVNGVSPDSMW
                     IQAVDPSGGSAPPGARRDALKIFMTNNRNPGTYTLDTSRLFNKPTLRFTHPPTQISVP
                     RFISGVETIFNDPPVLSCVPYRGVYFGAAVSILPEQASDPESDPLTFSLVSFSKTGGG
                     GPPLNAPTVDASTGRFSWPTAADVAELGTWEATIAVTDLPIRPPLTCTVPIEVTDLRG
                     DVNND"
     gene            complement(958..1929)
                     /locus_tag="AC16FU_01265"
     CDS             complement(958..1929)
                     /locus_tag="AC16FU_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993041.1"
                     /translation="MLRYQMKRRGMCLRWLAKANLIMGGIFLASAVKPAQAGPFVDSL
                     IVESKTFGPASQGAIEIFVSNDSAVTSIQLPLVIHSLSGGAFARIDSVKQLFTSPLPP
                     GTRTPVFRAFVTNNSGLGTYAVDSGGPLLVIHTGGQPNVPFFVRGVETIGTQAPILRC
                     SPGRTVYFGASVSLLPLEASDPEGDQLLFSLVGFSKIGGGGPPQNSPVVDVNTGRFFW
                     QTVSNATEVGTWETTLGVWDPLSFSPVTCTFTIEVTDLRGDVNNDGLWSDVVDIIELI
                     NLVVFQTSITSENPNSADVNCSGVVDLEDIVALISFVVFGAPAPCRG"
     gene            complement(2039..2121)
                     /locus_tag="AC16FU_01270"
     tRNA            complement(2039..2121)
                     /locus_tag="AC16FU_01270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2086..2088),aa:Ser,seq:tga)
     gene            complement(2365..3618)
                     /locus_tag="AC16FU_01275"
     CDS             complement(2365..3618)
                     /locus_tag="AC16FU_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR2993042.1"
                     /translation="MLILYLLLSLFFLFLGYAATAATAELYRDPQELEKLTADQKERR
                     FLKRVLARPQETLFVASAFRALLFLTLTVLTLEIQRLASAGLPGFSWVWYFAGFLALW
                     LFWLLTVEVFPKFRLLSGQPSKLIRYLWLVGPLVILFKPLSALLFPPRERRTHDPELE
                     EKKEEMVERALETLAETSGVDEPLIEQEEKKMISQILRLDTTAVKEIMIPRVNIVWVN
                     HTASFDEVKEIVKTSGHSRLPVCRETIDDILGILYIKDLIHAAIIEPTPDWSQYVRKS
                     YFVPESKKIDELLDELRSRKTHMAVVVDEYGGTAGIVTLEDILEEIVGEIHDEYDVGE
                     PEIQKVDDQTFRVTGTVNVQHLAETVGVDLPEEKFETVGGLIYDLVGSLPEEGKVVEH
                     AGLRFKVEKVVGQRIKKILVARTSH"
     gene            complement(3622..3999)
                     /gene="ybeY"
                     /locus_tag="AC16FU_01280"
     CDS             complement(3622..3999)
                     /gene="ybeY"
                     /locus_tag="AC16FU_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR2993043.1"
                     /translation="MVLAPFRPLVRSILKGEKRAGDITIVFITDERMADLNFRFRRKN
                     RTTDVLAFPLNDKLAANGRVQGEIYIALDQARRQAKEYGATLAEELKRLVVHGTLHLL
                     GYDHHRRAQARRMRDKEARYLFA"
     gene            complement(4099..4848)
                     /locus_tag="AC16FU_01285"
     CDS             complement(4099..4848)
                     /locus_tag="AC16FU_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR2993044.1"
                     /translation="MNERYLLYAIAMVKENGAGPADQLQLNALITSLSKEPVQPKTVG
                     QKAYVQAMAQYDIVFSIGPAGTGKTYLAVAMAVAELKKKMVNRVILVRPAVEAGESLG
                     FLPGDIRAKVDPYLRPVYDALHDLLAGEKIRKLIEAGIIEIVPLAFMRGRTLNSAFVI
                     LDEAQNTTNAQMKMFLTRLGNNSKAVITGDITQIDLNDKGASGLVQIQKILTGIPGIK
                     FVYLTEKDVVRHRLVQDIILAYERYENKKSK"
BASE COUNT         1103 a         1419 c         1302 g         1075 t
ORIGIN      
        1 ttgcagttca catctgccgc gttcggattc tcggaagtga tgggaatctg aaacactacc
       61 ttattgatca gttaaatgac gtccacaaga tcactccata atcagtcatt gttcacgtcc
      121 ccccgcaggt cggtcacctc tatgggaact gtgcaggtaa gcggcggcct gataggcaaa
      181 tccgtaaccg cgatagtcgc ctcccacgtc ccaagctctg cgacatcggc ggcggtcggc
      241 caggaaaagc gtcccgtgct ggcgtcgact gttggtgcat tcagaggcgg cccgccacct
      301 cccgttttgc tgaaactgac cagcgagaac gtcaacggat cactctccgg atcgcttgcc
      361 tgctccggca gaatagatac cgccgcccca aagtaaactc cacgatacgg gacacagctc
      421 aagaccggag ggtcattgaa aatcgtctca acgccgctaa tgaagcgggg aacactgatc
      481 tgcgtcggtg gatgggtgaa cctcagggta ggtttattga atagccggct agtgtcaagc
      541 gtgtaggtac cggggttcct attgttagtc ataaagattt ttaaagcgtc ccgccgggca
      601 ccgggcggag ccgaaccgcc gctcggatct accgcttgaa tccacatgga atccgggctt
      661 acgccattca ccttagtcag attaagggtt atccccaatg ggttaagtga aggcaacgcc
      721 tggaccgagt ccaccttgcc gaaagccccg ccggtggtcg aactgattag gaaggacata
      781 acccacccgt tcatggcgga atcattggag atggccactt caatgacacc tatgctgcca
      841 gtgaaagtct tggaagcaat aatcagcgaa tcgacaaacg gcccggcgct cgcgcttgag
      901 ctgaatagca aaagtagtac cgcaaatccc aggtatttcc tattcatacc gtcctcacta
      961 tccccggcac ggtgccggtg ccccgaagac cacgaaactg atcaacgcga caatatcctc
     1021 taagtccacc accccactgc agttcacatc cgcactgttc ggattctctg atgtaatgga
     1081 agtctgaaaa actaccaaat tgatcagctc aataatatcc accacgtcac tccacaaccc
     1141 gtcattattc acgtcgccgc gcaaatcggt tacctcaatc gtgaaagtac atgttaccgg
     1201 agaaaatgat agaggatccc aaacaccaag ggtcgtctcc cacgtgccaa cctctgtggc
     1261 gttactgacg gtctgccaga aaaagcgacc ggtattaaca tccactactg gtgaattctg
     1321 aggtggccca ccaccaccta ttttgctgaa gccgaccaac gaaaacaaaa gctggtcgcc
     1381 ttccgggtca ctcgcttcta atggcaggag cgaaactgac gccccgaagt agaccgtgcg
     1441 acctggggaa caccgcagaa ttggagcttg agtccctatt gtctcaactc ccctgacaaa
     1501 aaacggtaca ttcggctgcc cgccggtgtg aatgacaaga aggggaccgc cagagtccac
     1561 tgcataggtc cctagaccgc tattattagt gacgaacgcc cggaacaccg gcgtgcgggt
     1621 tcctggtggc aaagggctcg tgaaaagctg cttcactgag tcgattctcg caaaagcacc
     1681 gcccgaaaga gaatggatca ccagcggcaa ctggatactg gtaacggcac tgtcattact
     1741 gacgaaaatt tcaatagctc cctggctcgc gggaccgaaa gtcttggact ccacaattaa
     1801 ggaatcgacg aacggcccgg cctgtgccgg ctttacggct gatgcaagga atattcctcc
     1861 cataataagg ttggcttttg ccagccagcg taagcacatg ccccggcgct tcatttggta
     1921 ccgcaacata agcgctcctt tcactcgctt ggtggagaac cgcgagccct tccgggtggc
     1981 gaaccggtat gctaaatata actcctgttt tataatgagg caatcaattt cagcagaacg
     2041 gagggtgagg gactcgaacc cccatgacct tgcggtcgcc agatttcaag tctggtgcct
     2101 tgccaattag gctaaccctc cagaggagag ggtaacatgc cggagaggaa aaaacaaggt
     2161 gggcccgtat ctttagatgc ggcagagcct cttctggaca agcccgttac agaaccggcc
     2221 ttaaggccgg ttttttagac ccacaaatcc tttccgtttc tatagcagtt tgacccctcc
     2281 ccatacgggc cgtatggcct gccctcccct caaggggagg gaaaggtcag taaggggagg
     2341 ggaaaggtca gtttttgatc gtccttagtg agaagtccgc gcaactaaaa tcttttttat
     2401 cctctgcccc accacttttt caactttaaa cctcagcccc gcatgctcca cgactttccc
     2461 ctcttccggc aaactgccga ccagatcgta aatcagcccg ccgaccgttt caaatttttc
     2521 ctcgggcaga tcgacgccga ccgtctcggc caaatgctgg acattgaccg tcccggtcac
     2581 ccggaaggtc tggtcgtcca ctttttgaat ctccggctcg cccacgtcgt actcgtcgtg
     2641 aatttcgccg acgatttcct ccaggatgtc ctctaaggtg acgataccgg cggtaccgcc
     2701 gtactcgtcc accaccaccg ccatgtgggt cttccgcgag cgcaattcat ctaatagttc
     2761 gtcgattttt ttcgactccg gcacaaagta cgacttgcgc acgtactgcg accagtccgg
     2821 cgtcggctcg atgatggcgg cgtggatcaa atctttgatg tagaggatgc ccaggatgtc
     2881 gtcgatggtc tcccggcaga ccggcagccg cgagtgcccg ctggtcttga cgatctcttt
     2941 tacctcatcg aacgacgccg tgtgattgac ccagacgatg ttcacccggg gaatcatgat
     3001 ttctttcacc gctgtggtgt ccagccggag gatctggctg atcattttct tctcttcctg
     3061 ctcgattaag ggctcgtcca cgccggaggt ctccgccaga gtctccagcg cccgttccac
     3121 catctcctct tttttctctt ctaattccgg atcgtgggtg cggcgttccc gcggcgggaa
     3181 gaggagtgcg gaaagcggtt tgaacaggat gaccagaggc cccaccagcc acaggtagcg
     3241 aatcagttta ctcggttgcc cggagagcag acggaatttg gggaagactt ccacggtcaa
     3301 aagccagaaa agccagagcg ccagaaatcc ggcaaagtac caaacccaag aaaatcccgg
     3361 cagtcccgcg ctggcaaggc gctgaatttc caaagtgagg acggtgaggg tgaggaacag
     3421 aagcgcccgg aaagcactgg cgacgaagag cgtctcctgg ggccgggcca aaacccgctt
     3481 caaaaagcgt ctttcctttt gatcagccgt caatttttct aattcttgcg gatcacggta
     3541 gagttcggca gtggccgccg ttgctgcgta gcccaagaag agaaagaaaa gtgaaaggag
     3601 gagatagagg atgagcatgg gttaagcgaa aaggtaacgc gcttctttgt cccgcattcg
     3661 gcgggcctgg gcacggcggt gatgatcgta gcccagaagg tgcaaggtcc cgtgcaccac
     3721 cagccgcttg agttcttcgg ccaacgtggc cccgtattct ttcgcctgcc tgcgggcctg
     3781 gtccaaggca atgtaaatct caccctggac gcgcccgttg gcggccagtt tatcgttcaa
     3841 cgggaacgcc aggacatcgg tcgtgcggtt tttgcgccgg aagcggaagt tgagatcggc
     3901 catccgttcg tcggtaatga agacaatggt gatgtcgccc gcgcgctttt cgcctttgag
     3961 aatcgagcgc accaagggac ggaagggcgc caggaccact cttctttccg ggtgcaggtt
     4021 ggccacgagt atgcggctgg accccttttt tctgcgagga gactttggat gtgaactcat
     4081 tgccccaccc gtagccttct acttcgactt cttattctcg taccgctcgt aggccagaat
     4141 gatgtcctgc accagccggt ggcgcaccac gtctttttcg gtgaggtaga cgaacttgat
     4201 gcccggaatc ccggtcaata tcttttgaat ctgcaccaga ccggaggccc ccttgtcatt
     4261 cagatcaatc tgcgtgatgt cgccggtgat caccgccttg gagttattcc ccagccgcgt
     4321 gaggaacatc ttcatctgcg cgttggtggt gttctgggct tcgtccagga tgacgaaagc
     4381 gctgttgagg gtccgcccgc gcatgaaggc cagggggacg atttcgatga tccccgcttc
     4441 aattaacttc cgaatttttt ctcccgctaa aaggtcgtgc agtgcgtcgt agaccggccg
     4501 caggtaggga tcgaccttgg cccggatgtc gccgggcaaa aaacccaaac tctctccggc
     4561 ttccaccgcc ggacggacca gaatcacccg gttcaccatt ttcttcttca actcggccac
     4621 cgccatcgcc acagcgaggt acgtcttgcc cgtccccgcc gggccgatgg aaaagacgat
     4681 gtcgtactgc gccatcgcct ggacgtaggc tttctgcccc accgtcttgg gctggaccgg
     4741 ctccttggag agagacgtga tgagcgcgtt cagttgcagt tgatccgccg ggccggcacc
     4801 attttctttc accatggcaa tggcgtacaa aaggtagcgc tcgttcactt cctggttcgc
     4861 tttaaggcgg gtaacgaggt cggaaatgac ctactcaac
//
LOCUS       DBNGZA010000063         2647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2239286, whole genome shotgun sequence.
ACCESSION   DBNGZA010000063 DBNGZA010000000
VERSION     DBNGZA010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2239286"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="AC16FU_01290"
     CDS             <1..649
                     /locus_tag="AC16FU_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993045.1"
                     /translation="PEEGLAQFLMARALDPKSEFQKELSQRIVTSYERLGRTDEAKRE
                     LEKATALETKPVSAPEKEVVATIGKRQITRKELERELQFVPPEVRQNFPDTKEGRRNF
                     LNQYLGLELLYESAKRRGYADDSAAITYGQRAQKEYATERVITEDIAARVAVTPDEVK
                     KFYEQNKKKFGGKPFSAVADSVTSLVRANKQKELYLQTIQNLWEKEKVQVFDERL"
     gene            636..1622
                     /locus_tag="AC16FU_01295"
     CDS             636..1622
                     /locus_tag="AC16FU_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR2993046.1"
                     /translation="MSGCKKILSGPLGSVVTALWLPVVACAVPADSIKPQVQVTTTVD
                     RKEITIGDRLKYTITVERDPKVKVAPFPLGGNLGAFEVKDFKPLPEKKMKGRVVQTTE
                     FLVTTFTTGEYVIPPIAVSYTDTTGKKQLLYSDSIKITVKSVGRKASDTDDIRDIKPP
                     LAVGSETLPFVVGGVLFLLLAAAGYLWWRKRRTVAVVGQLPPVDTRPVWEIAKERLLI
                     LKNSGLLSENRIKEYHFQLSEIVRWYLERRFGILALEQTTEEIRAALKKIDFPRRLFS
                     TAIGLLDFCDLAKFAKYQPPATEIEVNWQVAFQVVEETTPKPEPTAPTEVGT"
     gene            1624..2613
                     /locus_tag="AC16FU_01300"
     CDS             1624..2613
                     /locus_tag="AC16FU_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2993047.1"
                     /translation="MRFASPWFLSLLVLLPAVIGYYLGRKRKEQPSLRYSYTGLFSGA
                     RASWRARLAWVSLALRSLMILLVVLALARPQKGRSSQEVESEGIDILLALDISSSMKA
                     EDFKPQNRLYVAKETIKDFIAGRKHDRLGLVVFARQAFTQCPLTLDYGVLLDFLHQVD
                     FGMVEDGTAIGMALITCVNRLKESQAKSKVIILLTDGVNNAGEVDPVTAAGAAQAFGI
                     RVYTIGVGQPGAALYPYDDPVFGRRYVYMPNEIDEASLAKIAELTGGRYFSAKTEKML
                     SEIYKKIEELEKTKIKVKEYRQYDERFWPFALGGLGFFLAEIFLANSLFRRMP"
BASE COUNT          621 a          696 c          776 g          554 t
ORIGIN      
        1 gccggaagaa gggctggcgc agttcctgat ggcccgggcg ctcgatccga aatcggagtt
       61 tcaaaaagag ctctcgcaga gaatcgtgac cagctacgag cggttggggc gaaccgacga
      121 agccaaaagg gagttagaga aggccaccgc gctggaaacc aaaccggttt ctgcgccgga
      181 aaaggaagtg gtggccacta tcgggaagcg gcagattacc agaaaagaac tcgaacggga
      241 attgcaattc gtgccgccgg aggtaagaca aaacttccca gacaccaagg aaggacggcg
      301 caattttctg aatcagtacc ttggtttgga attgctctat gagtcggcca aaaggcgggg
      361 gtacgctgac gattccgccg ctatcactta cggccagcgc gcccaaaagg aatacgccac
      421 ggaacgggtg attaccgagg acatagccgc cagggttgcc gtgacaccgg acgaggtgaa
      481 aaagttctac gagcagaata agaaaaagtt cggtggcaaa cctttttctg cggtggcgga
      541 ttcggttact tcgttagttc gggcaaacaa gcagaaagaa ctctacctgc agacgatcca
      601 gaacctctgg gagaaagaga aggtacaggt cttcgatgag cggttgtaag aaaatcctct
      661 caggaccact gggatcagtc gttaccgctc tttggctacc ggtggtggca tgcgccgtgc
      721 cagcggatag cataaaacca caggtgcagg ttaccacgac cgttgaccgc aaggaaatca
      781 ccatcggtga ccgcctcaag tacacgatca ccgtggaacg cgatccgaaa gtaaaggtcg
      841 ctccgtttcc gctgggaggc aatctggggg ccttcgaagt caaggatttc aagccactcc
      901 cggagaaaaa gatgaaggga cgtgtggtgc agacaacgga gttcctggtc accaccttca
      961 ctaccggaga atatgtcatt ccgccgatcg ctgtgtcgta caccgacacg accggaaaaa
     1021 agcagctgct ctattcggat tccatcaaaa tcaccgttaa aagtgtgggg cggaaagctt
     1081 cggataccga tgacatccgg gacatcaagc cgccgctggc tgtcggctcg gaaacgctgc
     1141 ccttcgtggt cggcggggtg ctctttttgc ttctcgcagc ggcgggctac ctctggtggc
     1201 ggaaacgccg gacggttgcg gttgtgggtc agctgccgcc ggttgacacc cggccagtct
     1261 gggaaattgc caaagagaga ctcttgattt tgaagaattc aggtttgttg tccgaaaacc
     1321 ggatcaaaga ataccacttc caactgtcag aaatcgtccg ctggtacctc gagcggcgtt
     1381 tcggcattct cgctctggag cagaccaccg aagaaatccg cgcggcgctc aaaaagatcg
     1441 attttccccg gcggcttttc tcaaccgcca tcgggctgct tgatttctgc gacctggcga
     1501 agttcgccaa ataccagccg ccggccactg aaattgaagt gaactggcag gtggctttcc
     1561 aggtggttga ggaaacaacg ccgaagccgg aaccaaccgc tccaacagag gtaggcacct
     1621 gaaatgcgtt ttgcgtcacc ctggtttttg agcctcttgg tgcttctccc ggcagtgatt
     1681 gggtattacc tggggcggaa aagaaaagaa cagccgtccc tgcgctattc ttacacgggt
     1741 ttgttctccg gggccagggc ttcttggcgg gcgcggctgg cgtgggtgtc tttggcgttg
     1801 cgttcactga tgattcttct ggtcgttctg gctttggcgc ggccgcaaaa gggccgctcc
     1861 agtcaggaag tggaatcgga aggtatcgat atcctattag ccctggatat ttcttcctct
     1921 atgaaggccg aagatttcaa gccgcagaat cggctctacg tcgccaaaga gacgatcaaa
     1981 gatttcatcg ccgggcgtaa acacgaccgc ctggggctcg tggtcttcgc gcgccaggcc
     2041 ttcacccagt gcccgctcac cttggactac ggtgtgctcc tggattttct ccaccaggta
     2101 gacttcggga tggttgaaga cggaactgcc atcgggatgg cgctcatcac ctgcgtcaac
     2161 cgcctcaagg agtcgcaggc gaaaagcaag gtgattatcc tcttgaccga cggggtaaat
     2221 aatgccggcg aggtcgatcc ggtgaccgcc gccggtgcgg cccaggcctt cggcatccgc
     2281 gtttacacca tcggagtggg tcagccgggc gcggcactct atccgtacga cgatccggtc
     2341 ttcggccggc gctacgtcta catgccgaac gaaatcgacg aagcgtcgct tgcgaaaatt
     2401 gcggagttga ctggcgggcg ctacttcagc gctaagaccg aaaaaatgct ttctgagatt
     2461 tacaagaaaa tagaagaatt agaaaagacg aagatcaaag tcaaagaata ccgccagtac
     2521 gacgagcgct tctggccgtt cgctttgggc gggctggggt ttttcctggc ggaaatattt
     2581 ttggccaatt cgcttttccg gaggatgccg tagtgcgttt cggacaacct ctgattctct
     2641 acttgta
//
LOCUS       DBNGZA010000064         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1602860, whole genome shotgun sequence.
ACCESSION   DBNGZA010000064 DBNGZA010000000
VERSION     DBNGZA010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1602860"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1772
                     /locus_tag="AC16FU_01305"
     CDS             189..1772
                     /locus_tag="AC16FU_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993048.1"
                     /translation="MQKVLCKTQLVWLAAGLSLLANGRSAGAASVPGPIRDVFNPARG
                     DSILIVYGTQGDSATTAYLKFLAARTFWIVANEKPELKMAVVPDTSITPPRGLEVSLV
                     VLGTKRSNRILKEYERPLQVRVLPDQVFIGLNQFLGKDYTVAFVHPSPFNPFKYLLVL
                     TGSSDSTLAMLPQIPDTFDFCLKQVHQYEKNRYYTAGFFAKGTLPWGLEEGAVKVTGK
                     YRHYRVLLSPKLRLRYPRDWDTLLTPPQKLWLLRTAQKTHDVFAITYGFNLPRPFDLC
                     LVDDLPGTTATDYARLYWKIDPLHLDTTVAPATGIALARFILSCPDAPSDWPTFVQST
                     GVAPATVFRRAKVIDSATVERPKPKEQVWFEEIVAPDTTYASILFAAANAYQPRALTS
                     VINQVTHTLENPFVPMSTLLERLAARTGDRVIAQLARQAIPLPYDKPIRIRTGIPNLA
                     DHLQKDRVLTLASLPSSGAAAAAGMKKGDTLLSIDGISLDFQRALAYQRLAGKKKGDR
                     ILFGIQRGEKTLNIPITAR"
     gene            complement(1843..2325)
                     /locus_tag="AC16FU_01310"
     CDS             complement(1843..2325)
                     /locus_tag="AC16FU_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR2993049.1"
                     /translation="MGCVVDATGCSVDTDGDGVCDGLDKCPNTPKGRPVDLTGCEFVL
                     PTPEKPLVLEGVHFETGKALLTEDSKQILARVAASLLDHPEVKLEVAGHTDATGSDAH
                     NLKLSQARANAVRNYLISKGVKAEVLTAKGYGKSQPIADNQTKEGRDRNRRTELRRLP
                     "
     gene            complement(2329..2385)
                     /locus_tag="AC16FU_01315"
                     /pseudo
     CDS             complement(2329..2385)
                     /locus_tag="AC16FU_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014466.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          491 a          764 c          669 g          523 t
ORIGIN      
        1 gttcggtaca caccgctccg tcgggcggtt ttcctcaaga tcgctaaccg gaaacacggg
       61 cccgccgtat ggtgagaata taagggtcag ccggtgctgg taaaagacgg ctcctttgac
      121 agatgcgcgt ggaagcggta aaataagcgc tcgtttgcga tatgagaaca aagtcgcgag
      181 gtaaaaaaat gcaaaaagta ctttgcaaaa cccaactggt ttggctggca gccggccttt
      241 cccttctggc caacggaagg tcagccgggg cggcgtctgt tcccggcccc atccgggatg
      301 ttttcaatcc ggcgcgcggt gactcgattc tcatcgtcta cggcacccag ggtgactcgg
      361 cgacgaccgc ctacttgaag tttctggccg cccggacctt ctggatcgtg gcgaacgaaa
      421 aaccggaatt gaaaatggcc gtcgtgccgg atacgagcat cacgccgccg cggggtctgg
      481 aagtttctct ggtggtgctg ggcacaaagc gctccaacag aatcctgaaa gaatacgaac
      541 ggccactgca ggttcgggtg ctacccgatc aggtgttcat aggactcaac cagttcctgg
      601 gcaaagacta cacggtcgcg ttcgtccacc ccagcccctt taacccgttc aagtaccttt
      661 tggtgctcac cggctcttcg gatagcaccc tggcgatgtt gccgcaaatt cccgatactt
      721 ttgacttctg cctcaagcag gtgcatcagt atgaaaaaaa tcgctactac accgcaggtt
      781 ttttcgccaa aggcaccctg ccctgggggt tggaggaagg agcagtgaag gtcaccggca
      841 agtaccgtca ttaccgggtg ctcctgtcgc ccaaactccg gctgcgctac cctcgggatt
      901 gggatacgct cctgacgcct ccccagaaac tttggttgct acgcactgcc cagaaaaccc
      961 acgacgtttt cgcgattacc tacggattca acctgccccg accgttcgac ctttgcttgg
     1021 tggacgacct tcctggcacg acggcgaccg actacgcccg cctctactgg aagattgacc
     1081 ccctccatct ggacacgact gtggcaccgg ccaccggcat cgccctggcc cgttttattc
     1141 tttcctgccc ggatgctccc agcgactggc ctacctttgt acaatctacg ggtgtggctc
     1201 cggccaccgt cttcaggcgc gccaaagtca tcgattcagc caccgtcgag cggcccaaac
     1261 ctaaagagca agtctggttt gaagaaatag tcgctcccga cacgacctac gccagtatac
     1321 tttttgccgc agccaacgct taccagccgc gggccttgac cagtgttata aatcaggtga
     1381 cgcacaccct tgaaaatccg ttcgtaccca tgagtacact tctggagcgg ttggcggcgc
     1441 ggaccggcga cagagtaatc gctcaactgg cccggcaggc gatacccctg ccctacgata
     1501 aaccgatccg catccgtacc ggtattccga acttggccga tcatttacaa aaagaccggg
     1561 tgctcacgct ggcttctttg ccctcctccg gcgccgccgc agccgccgga atgaaaaagg
     1621 gagataccct cctgagcatt gatggcatct ccctagattt ccagcgcgct ctggcgtacc
     1681 agcggctggc gggaaagaaa aaaggcgacc ggattctctt tggcatccag cgcggggaaa
     1741 agaccttgaa catcccgata actgcccgct aaaaaaccca gaatcccgtc ttggggacgg
     1801 gattctgggt ttgataagtc agttccgctt taaatcgccg gtctaaggca gccgccgcaa
     1861 ttccgtccgg cggttccggt cccgcccttc tttcgtctgg ttatccgcta tgggctggga
     1921 cttcccatat cctttggcgg tgagcacctc tgccttgacc cctttgctga tgaggtaatt
     1981 ccgtaccgcg ttcgcccgcg cctgcgagag tttcagattg tgcgcgtcgg agccggtggc
     2041 gtcggtgtgt ccggctactt cgagtttcac ctccgggtgg tccaacaaac tggcggcgac
     2101 acgggccaga atctgcttgg aatcctcggt gagcaacgct ttgccggtct cgaagtgcac
     2161 gccctccagc accagcggtt tttcgggcgt ggggagcaca aactcacagc cggtcaggtc
     2221 caccggtctg cctttgggtg tgtttggaca tttgtccagg ccgtcgcaga cgccgtcgcc
     2281 gtcggtgtca accgaacagc cggtggcatc aacaacgcaa cccaccggtg tccccgggca
     2341 atggtcaatc ccgtctggta caccatcttt gtcgccgtcc aactcgcatc attttgcatc
     2401 cacggtggcg ccggccggcg tgctcgggca gcggtcgatc ccgtcgc
//
LOCUS       DBNGZA010000065         6599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2663247, whole genome shotgun sequence.
ACCESSION   DBNGZA010000065 DBNGZA010000000
VERSION     DBNGZA010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2663247"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="AC16FU_01320"
     CDS             <1..1123
                     /locus_tag="AC16FU_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR2993050.1"
                     /translation="VEGLNGTAEIDNQMARIQELSFKSGKSSFTLTGTLVRFVPLLFS
                     KAPGQPKPLFTFRLDAPFFDYDEMYPTRRNAAGKVIISTDTVPLPDIEARGKAAVKRG
                     VYSQVEFTDFTADVHYLNRLLTLDNARMTVYGGGAAGKATVDLHDQLHPLFAIAAKAD
                     SIEANGFLTRFSFFRDRLFGRMAFNGTFSSRGLLNDEIVRHLSANGKAQMTGARLVRW
                     PLVTQLGAFLNLNLQEEQKIRDLASRFAVENGKVAFDAVTFATGDGDFALAGSVGLDG
                     SLDYRLTTILSPENSQKFRLSSGLKQLFTTDDGRVVLDLNIGGTATDPAFALDFSRAE
                     KKAQEQIQQKLQEKKEEVGKSLREKGQDLLQGLFKKKKP"
     gene            1137..2063
                     /locus_tag="AC16FU_01325"
     CDS             1137..2063
                     /locus_tag="AC16FU_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR2993051.1"
                     /translation="MRFSLGASALVFTLIAQTTFAAPYIQFASRPEDSAFVRQTHQAL
                     LTGVQRLEQFAPGAFPETIFVFVAATPEEFDQYSLFHPPDWGAAVAVPALNRIVLKSP
                     KIAPGGQPLEEIVTHELAHLYLYRLAGRAHLPRWIDEGFASWQSGEWSYGQSLVLARA
                     NLSNSLIPLEEIDEVLKFRPAKAALAYAQSHLAVQYFVDSYGKGAFVELAHGLGKSRR
                     PDSVFFAILGIDRQGYEAEYLAYLKKHYNWVAILSDFTVLWIFLAFLLILAFILYWRR
                     KRKILKAWQKKESFEQPRWSEPTDEFNPEDLP"
     gene            2005..2943
                     /locus_tag="AC16FU_01330"
     CDS             2005..2943
                     /locus_tag="AC16FU_01330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2993052.1"
                     /translation="MSNPGGPNRRTSLIQKIFRDLIFYKIVVTAWWLRRLPYLWALAL
                     GRLWGLLVFDLFRIRRAVTLNNLRRALGREKPKAEILSIARRTYQNLGQAFVEFCLIP
                     RLVRKRRLSGLVTSETFQFVQQAASGGKGCLLMSGHFGNWELSGGFICQSGLPVDFVV
                     KEIRNPKLDRWVSRYRQLLGVGRIPMDKAPREVITATRTGRCVAMLADQYAGAEGVIV
                     DFFGLPTSTPKGPAALALKLDIPIVCGFLIREGGGRFRLIVEPPIRPSPTGDRERDIQ
                     RLTQEFTRILEKYVRLYPDHWLWTHRRWKDLDRKEN"
     gene            3040..4239
                     /locus_tag="AC16FU_01335"
     CDS             3040..4239
                     /locus_tag="AC16FU_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2993053.1"
                     /translation="MRRYWKIGAGIVALIIVIIIAASIFSGKVDLPTAAAQSGEFVIT
                     LTENGELKAQRAVSVVAPRVRGKMHITKLAPEGSVVKAGDFLIQFDASEQHATLQDNQ
                     ANLKIAQANLNRALADLELQKQQLELDLEKAERNAKEKAYEAPAIKQEAEKELELARL
                     KKETQLKLLEADIEKLRVEVDRAKDKVTTAQNDLAQMTLKAPIPGLVVYMDIWKGNQM
                     AKVQEGDSPWPGQELINLPDLSAMQVKTSVSEVDVDKLKLGQETNIKLDAYPEPTFHG
                     KVTKISTLARRKDWNSRINVFDVEVTLDSADPKMKPGMSAKADIVVEKIPNSIWVPIE
                     AVFEKEGKTVVYLMGSRSKKVEVEVGKRSDTHVAITKGLKGGEKVALVDPTQKLEMLG
                     VKKEEKK"
     gene            4370..4978
                     /locus_tag="AC16FU_01340"
     CDS             4370..4978
                     /locus_tag="AC16FU_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993054.1"
                     /translation="MSEEREEIQSITCRACREINTSGAQFCAHCGRSLEEKSDVDKKQ
                     ISEGTVGGVIFIVVLIFGLIYVLFGHSSKDSDSSVKPSTPFSSETTTTPSLSSSSPVK
                     KLGFVPTTEQVKEARRIVHKAEEVGLVYKIDLQLQSVWVDSDLWNLLTYDVKEVYALS
                     FAYFISDENSTTKNISWIGIRDKYTGKEIASYGSTWGFRVKN"
     gene            5052..6353
                     /locus_tag="AC16FU_01345"
     CDS             5052..6353
                     /locus_tag="AC16FU_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993055.1"
                     /translation="MDYREVVQVALDSIVTHKLRSALTTLGVVFGVAAVIAMLSIGEG
                     AKQEALEQIALLGTNNILIKATTPPKSSGSEEVEGRSEGLSLADARNIEALSHLVTAV
                     VPERWEQIDRANFGDKESKVEVIATTPDYLALANQRLRRGSFFSAEDNTEGRRVCVIG
                     SEVKRNLFAFDDPVGKPIKLGDDYYTVLGVTEEKSIGKPKVEGLEVKNVNQQVYLPLG
                     TAQNRLERGGGGEGFYAFGGVTIFSSEGGGTKASEVDVLTVKVSDKEQIQPVAHVIKK
                     LLERRHRGVADFEIVIPEALLYQSQKTQRIFNIVMGAIAGISLLVGGIGIMNIMLASV
                     YERTREIGVRRAVGARRNDVLRQFLVEAVAISFVGCLLGLILGFGLARSVSYYAKWRT
                     IISLFSIVLAVGVSALVGIAFGYYPARRAAGLDPIEALRYE"
     gene            6346..>6599
                     /locus_tag="AC16FU_01350"
     CDS             6346..>6599
                     /locus_tag="AC16FU_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993056.1"
                     /translation="MSRNTVASSQGPVARKKSRQTLGQCLNATLVIALLLVVPLFAAI
                     AQEPVKLTLSECIQMALKQGKEAQIARSELEAAKSTYQARR"
BASE COUNT         1558 a         1738 c         1875 g         1428 t
ORIGIN      
        1 cgtagaaggg ctgaacggca ccgccgagat cgacaatcaa atggcgcgca tccaggaact
       61 ctccttcaaa agcggcaaat cttcttttac gctcaccggc acgctggtgc ggttcgtgcc
      121 tttgctcttt tccaaagcac cgggccagcc gaagccgctt tttaccttca ggctcgacgc
      181 gccttttttc gattacgatg aaatgtaccc cacccggcgg aatgcggcgg ggaaagtgat
      241 tatttccacc gatacggttc cgctcccgga cattgaggcg cgggggaagg cagcggtcaa
      301 acgaggcgtt tacagccagg tggaattcac cgacttcacc gctgacgtcc attacctcaa
      361 tagacttttg actttggaca acgcccggat gaccgtctac ggcggcggcg cggccgggaa
      421 ggccacagtc gacctgcacg accagctgca tccgctgttc gccattgcgg cgaaagccga
      481 ttccattgaa gccaacggtt ttctcacgcg cttttccttt ttccgggacc ggctctttgg
      541 aagaatggct tttaacggca cgttcagttc tcgggggctt ttgaacgatg aaatcgtccg
      601 gcatctctcg gccaatggca aagcgcaaat gaccggcgca cgattggtcc gctggccgct
      661 cgtgacgcag ttaggcgctt tcctcaatct caatcttcag gaagaacaga aaatccgcga
      721 cctggcaagc cggttcgctg tggagaatgg aaaggtggct tttgacgccg ttaccttcgc
      781 caccggcgac ggcgactttg ccctggccgg gtcggtggga ctggatggct cgctcgatta
      841 ccgtttgacc acgattctct ctccggaaaa ctcgcaaaag ttccgtctct ccagcggcct
      901 caagcagctc ttcaccaccg acgacgggcg ggtggtgctg gacctcaaca tcggcggcac
      961 ggcgaccgat ccggcgtttg ctctggactt cagccgggcg gagaagaagg ctcaggagca
     1021 aattcaacaa aagttgcagg agaaaaaaga ggaggtgggg aagagtttgc gcgaaaaagg
     1081 tcaagactta ctccagggtt tatttaagaa aaagaagccg tgaggggctg gcttccttgc
     1141 gattctcttt gggtgcctct gctctcgttt tcaccctgat tgcacaaacg acttttgcag
     1201 ctccatacat tcaatttgcc agccgcccag aagactccgc ttttgtccgg cagactcacc
     1261 aggcgctttt aaccggcgtt cagcggctgg aacagttcgc tccgggtgct tttcccgaaa
     1321 cgatattcgt ctttgtcgcc gccacgccgg aggaattcga ccagtattca ctcttccatc
     1381 cgcccgactg gggcgcagca gtggcggtgc cggctctgaa ccggatcgtc ctgaaatctc
     1441 cgaagattgc cccgggcggt cagccgctgg aggaaatcgt cacccacgaa ctggcgcacc
     1501 tgtatttgta ccgcctggcc gggcgcgccc acctgccgcg ctggatcgat gaggggttcg
     1561 cctcctggca gtcgggcgag tggtcgtacg gtcagtcttt ggttctggca cgggcgaatc
     1621 tctcgaactc cctcattcct ctcgaggaga ttgacgaggt gctcaagttt cgacccgcga
     1681 aagcggccct ggcgtacgca caaagtcatc tcgccgtgca gtacttcgtc gattcctacg
     1741 gcaaaggcgc tttcgtggaa ctggcgcatg gattgggaaa atcgcggcga cccgattcgg
     1801 tcttctttgc cattttgggg attgatcgcc aagggtacga agccgagtat ctcgcctacc
     1861 tgaaaaaaca ctacaactgg gtggcgattt tgagcgattt cacggtactc tggatttttc
     1921 tggcgttcct gctcatcctg gcttttattc tctactggcg gcgcaaaagg aaaattctga
     1981 aagcgtggca gaaaaaggaa tcctttgagc aaccccggtg gtccgaaccg acggacgagt
     2041 ttaatccaga agatcttccg tgatctaatt ttttacaaaa tagtcgttac cgcctggtgg
     2101 ctgcgacgcc tgccttacct gtgggcattg gcgctgggcc gtttgtgggg acttctggtt
     2161 tttgatctct tccgcatccg gcgcgcagtg accttaaaca acctccgccg cgctctcggt
     2221 cgggaaaaac ccaaagccga aatactctcc atcgcccgcc gcacctacca gaacctcggc
     2281 caggccttcg ttgagttttg cctgataccg agactggtca gaaagagacg actttccggc
     2341 ctggtcacct ctgaaacttt tcaattcgtc cagcaggccg catcgggcgg gaaaggctgc
     2401 cttctgatgt ccggccattt cggcaactgg gaactttcgg gcggtttcat ctgccagtcg
     2461 ggtcttccgg tcgatttcgt ggtcaaggaa atccgcaatc cgaaactcga ccgctgggtc
     2521 agccgctacc gtcagctctt gggagtcggc cgcatcccca tggacaaagc accgcgggaa
     2581 gtcattacgg ctacccgaac cggccgctgt gtggcgatgt tggcggatca gtacgcgggc
     2641 gcagaagggg tgattgtcga cttctttggt ctgccaacct cgactcccaa aggcccggcg
     2701 gcgttggcgc tcaaacttga cattcccatc gtctgtggtt tccttatccg tgaaggaggt
     2761 ggtcgcttcc gcctgattgt cgagccaccg attcggccct ccccaactgg tgaccgagag
     2821 cgcgatatcc agcgcctgac gcaagagttc acccgaattc tggagaaata cgtccgctta
     2881 taccccgacc actggctttg gacacaccgg aggtggaagg acctcgatag aaaagagaat
     2941 tgagaactgg agagagaaag agtgtagtga cgctctcccg agcgtcggga aaagtttagt
     3001 ctaaaggaat tgttaatggg tataactcta ccagacatca tgcgacgcta ctggaaaatc
     3061 ggtgcgggca tagtcgccct tatcattgtc atcattattg ccgcgtcaat tttttccggc
     3121 aaggttgacc tgcccacggc ggcggcgcag tcgggcgaat tcgtcatcac cttgaccgaa
     3181 aacggcgaac tgaaagccca gcgcgccgtc tccgtggtgg ccccgcgtgt ccgggggaag
     3241 atgcacatca ccaaactcgc gccggagggg agcgtggtca aagcgggcga tttcttgatt
     3301 caattcgacg cctccgaaca acacgccacc ctgcaggaca accaggccaa cctgaaaatc
     3361 gcccaggcca atctcaaccg cgccctggcc gatttggaac tccagaagca gcagctggag
     3421 ttggacctcg aaaaagccga gcggaacgcc aaggagaaag cctacgaagc tccggccatc
     3481 aagcaggagg ccgaaaaaga gttggaactc gcccgcctga aaaaagaaac ccagttgaaa
     3541 cttctggaag ccgacatcga aaaactgcgc gtggaagttg accgcgccaa agacaaagtc
     3601 accaccgctc agaacgacct ggcccagatg accctcaaag cgcccattcc cgggctggtg
     3661 gtctatatgg atatctggaa aggaaaccag atggccaagg tgcaggaggg tgactccccc
     3721 tggcccggcc aggagttaat caacctcccc gatctctccg ccatgcaggt caaaacttcg
     3781 gtctccgaag tggacgtgga caaactgaaa ttagggcagg agacgaacat caagttagac
     3841 gcctacccgg agccgacctt tcacgggaag gtgaccaaaa tcagcacgct ggcgcggcgg
     3901 aaagactgga actcgcgcat caacgtcttc gacgtcgaag tcactctcga ctccgccgac
     3961 ccgaagatga agccggggat gtcggctaag gcggacattg tcgtggagaa aatccccaac
     4021 tcgatctggg tgcccattga agcggtgttt gaaaaggaag gcaaaaccgt agtctacctg
     4081 atgggctccc gctcgaagaa ggtcgaagtt gaagtgggca agcgctccga tacccacgtg
     4141 gcgattacga agggcctgaa aggcggagaa aaagttgccc tggttgaccc gacgcagaaa
     4201 ttggaaatgc tgggtgtgaa gaaggaggaa aagaaataag aaagggaccc aagcaaggac
     4261 ctgagaatgt tttattacaa ttaagattgg tataatttca ttcgaaaggc accctgaagg
     4321 aaagtggaat ggaagcaaag gggattgaat tgataaagta ggatatacga tgtcagagga
     4381 gcgtgaggaa attcagagta ttacgtgtcg tgcctgtcgg gaaataaata cttcgggggc
     4441 tcaattttgt gcgcactgtg gtcgttccct agaagaaaag agtgatgtag ataaaaaaca
     4501 aatatcggaa ggtacagtag gaggagtgat tttcatagta gttcttattt ttggtttgat
     4561 ctacgtattg tttggtcact ctagtaagga ttcagattct tccgtcaagc cttctacacc
     4621 tttttcgtcc gagaccacga ccactccttc cttatcttct tcctctcctg taaaaaagtt
     4681 gggatttgta ccaacaactg agcaagtaaa agaagcaaga agaatagtgc acaaagcaga
     4741 agaagtagga ctagtataca aaatagattt gcaattgcaa tcagtatggg tggattctga
     4801 tttgtggaat ttgttaactt atgacgtaaa agaagtatac gcattaagtt ttgcttattt
     4861 catttcagat gaaaattcca cgacaaaaaa tatttcgtgg attggcatta gagacaaata
     4921 tacaggaaaa gaaatagctt cgtatggaag tacctgggga tttagagtga agaactgagg
     4981 tagtaaccat agaacggaga atagttaaaa tttttgacgt gttctctgtg tctccgtggt
     5041 gaaaaggttt tatggactac cgcgaagtcg ttcaggttgc gcttgactcc atcgtcacgc
     5101 acaagctgcg ctcggcgctt accactctgg gtgtggtctt cggcgtggcg gcagtgattg
     5161 cgatgctttc cattggcgag ggggccaagc aagaggcgtt agagcaaatc gcacttttgg
     5221 gcaccaataa cattctcatc aaagccacga ctccgcccaa atcatccggc agcgaagagg
     5281 tggaaggccg ctctgaaggg ctttctttag ccgatgcgcg gaatattgag gcgctgagcc
     5341 acctcgtcac cgctgtggtg ccggagcgat gggagcagat cgaccgggca aacttcggcg
     5401 acaaagaatc gaaagtggaa gtcatcgcca ccacacccga ttatctcgcc ttggccaacc
     5461 agcgcctgcg ccgggggagt ttcttttctg ccgaggataa cacggagggg cggcgggtct
     5521 gcgtcattgg ctcggaagtc aagcggaatc tcttcgcatt tgatgacccg gtgggaaagc
     5581 caatcaagtt gggagatgac tactacaccg tcctcggtgt caccgaagaa aagtctatcg
     5641 gcaagccgaa agtcgaaggg ctggaggtca aaaacgtcaa ccagcaggtc tacctaccgc
     5701 tgggcacggc gcaaaaccgc ctggagcgcg gcggcggggg agaagggttc tacgccttcg
     5761 gcggggtcac cattttctcc agtgaaggcg gagggacaaa agcaagtgaa gtagatgtgc
     5821 tcaccgttaa agtgagcgac aaagaacaaa ttcagccggt ggcgcacgtc atcaaaaaat
     5881 tgttggaacg ccggcatcgg ggcgtggccg acttcgaaat cgtcatcccc gaggcgcttc
     5941 tgtaccagag ccagaaaacg caaaggattt tcaacatcgt gatgggcgcc atcgccggga
     6001 tttcgcttct ggtgggcggc atcggcatta tgaacatcat gctggcgtcg gtctacgaac
     6061 ggacgcggga aatcggcgtc cgccgggccg tgggcgcgcg caggaacgac gtcctgcggc
     6121 agtttctggt ggaagcggtg gcgatcagtt tcgtcggctg tcttctggga ttgattctgg
     6181 gcttcggcct cgcccgctcg gtttcgtact atgccaagtg gcggactatc atctcacttt
     6241 tctccattgt tcttgcggtg ggcgtctccg ctctggtggg cattgccttc ggctactacc
     6301 cggcccgacg ggcagccggt ttggacccga ttgaggcgct gcggtatgag taggaataca
     6361 gtagccagta gccaggggcc ggtagccaga aagaagagtc gccagacatt ggggcaatgc
     6421 ctcaatgcaa cgctggtaat tgcgttactc ctggtggttc cactttttgc cgcaattgca
     6481 caggagccgg tcaagctcac actttccgaa tgcattcaga tggcgctgaa gcagggcaag
     6541 gaagcgcaaa ttgccaggag cgaattggaa gcggccaaat cgacctacca ggcacggcg
//
LOCUS       DBNGZA010000066         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2663438, whole genome shotgun sequence.
ACCESSION   DBNGZA010000066 DBNGZA010000000
VERSION     DBNGZA010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2663438"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..813
                     /locus_tag="AC16FU_01355"
     CDS             73..813
                     /locus_tag="AC16FU_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR2993057.1"
                     /translation="MFQQEVSLLAAFGAGIISFVSPCVLPIIPGYLSFVSGVSVEELQ
                     GGENRGRLLKRVSLTVLFFILGFSAVFVALGASASAFGLFLATHRSLFNKIAGVIIFI
                     FGLHLLGILRIPFLNYEARFQARTRPLGLVGAFGVGLAFAFGWSPCLGPILGAILGIA
                     ANQETVGQGMLLLSSYSLGLGVPFFLAAIGFNSFLSVSRWVKKHFRAIEITSGAFLVL
                     VAVLIFTDQLAVIARVLLRWFPWLNEIG"
     gene            987..1598
                     /locus_tag="AC16FU_01360"
     CDS             987..1598
                     /locus_tag="AC16FU_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2993058.1"
                     /translation="MKDSSHPASLPAAPPDGKEFEQAVFAYVAPLYRSALRLTRNKSD
                     AQDLVQDTVLKAYRFFHRYQRGTNLKAWLFKIMTNLYINAYREKARKPQEVTIEDVEE
                     LYLYHRLTETRRPGEANPEETFFGQLYEDEVRRVLDELPADYRLVVVLAFVEEFSYEE
                     IAQITRLRLGTVKSRLFRGRKLLQKKLWDYAVRAGYLKRKREA"
     gene            1595..1831
                     /locus_tag="AC16FU_01365"
     CDS             1595..1831
                     /locus_tag="AC16FU_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR2993059.1"
                     /translation="MNCAQALSYLYEYVDRHLPAPLRTEVQAHIAICRGCTRRFGIEE
                     SLRAVVGRSGRVVAEIAGLKVALRQKLAAVRGKG"
     gene            complement(1944..2066)
                     /locus_tag="AC16FU_01370"
     CDS             complement(1944..2066)
                     /locus_tag="AC16FU_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993060.1"
                     /translation="MLVSTIDSCGHHRLLALKYKARKRFVNAGLRLRKKKLKGG"
     gene            2102..2833
                     /locus_tag="AC16FU_01375"
     CDS             2102..2833
                     /locus_tag="AC16FU_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR2993061.1"
                     /translation="MRNRKEALVVFVRAPHYGELKARLAQELAPAEVESLYRAFVGDT
                     VMLCSRVNDAQLVVGFTPPEARREVTGFVEEALLAANHQKKPVPGPLVFSEQTGANLG
                     ERLRAMFALLFRQGYHEVVIIGADSPTVPPETLRKAFVLLKKKPVVLGPTLDGGYYLV
                     GLARPAPEIFDRIAWGTDQVYPQTVEKLKSLGLEWEELPVWYDVDTVDDLEFLVRDIN
                     QLRLAGDEESLAATEVVLEKILNKS"
     gene            2852..3577
                     /locus_tag="AC16FU_01380"
     CDS             2852..3577
                     /locus_tag="AC16FU_01380"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR2993062.1"
                     /translation="MKLEALTWQDFKRLVPSKIDTALLPVGTIEAHGVATLATDNLIP
                     ETLAQRMADKVKALILPTVPYGITRSLFGYPGSFTISPQNFTAYLVDIFRSIATQKFR
                     RLVVLNGHGGNNDALKQAALDINHTTGLKIAVIHWWTICQKVCREVYGEAGGHAALDE
                     TAFVLAVKPEYVKKNQYKKEMAYLMTEGGADIYPNPGSIILYEPGQGYLNFDPEKAQQ
                     YVEGCVKEVTRFVLDIFVRWKKI"
BASE COUNT          759 a         1049 c         1125 g          795 t
ORIGIN      
        1 cggttgcagc gttgtatgtt catttcctaa agccgggccg gccgaagcct gcccggcgga
       61 caaggagcag agatgtttca acaggaagtt tcgctattgg ccgccttcgg cgcgggaatc
      121 atttccttcg tttcgccctg cgtcctgccc atcatccccg ggtacctttc gtttgtctcc
      181 ggcgtatcgg tggaggagct gcagggtggg gagaaccggg gacggctcct gaagcgggtg
      241 agcctcaccg tcctcttttt catcctgggg ttctcggcgg tgttcgtcgc tttaggcgca
      301 tcggcctcgg ccttcggact gttcctggcc acgcaccggt cgctcttcaa caagatcgcc
      361 ggggtgatca ttttcatctt cggtctgcac ctgttgggaa ttctgcggat tccgtttttg
      421 aactacgaag cccgcttcca ggcccgcacc cggccgttgg gattggtcgg cgcttttggc
      481 gtgggattgg ctttcgcctt cggttggtca ccctgcctgg ggccgatcct gggggctatc
      541 ctgggcattg ccgccaatca ggagacggtg gggcagggaa tgctcctctt atcgagttac
      601 tctctggggc tgggcgtgcc cttctttctg gcggccatcg gcttcaactc gtttctctcg
      661 gtgtcgcgct gggtgaaaaa gcattttcgg gcgattgaaa tcaccagcgg cgcctttctg
      721 gttctggtgg cggtgctcat ctttaccgac cagctggcgg tcatcgcccg ggtcctcctg
      781 cgctggttcc cctggctgaa cgagatcgga taaataccat tgaacctgct ccggtcggga
      841 tcgactcgta ccgaggagcg ctttttccgg ttccccgctc cgcaggcggg aggggaactt
      901 ttcattttcg gctccggttg taggaaatgg cgcgggccgt ggccccggcc cagagcggag
      961 cggaggcgga gagataacag cgtaccatga aagactccag ccaccccgcg tctctccctg
     1021 cggcaccgcc cgacggaaaa gagttcgagc aggcggtctt cgcctacgtc gcaccgctct
     1081 accggagcgc tttgcggctg acgcgcaaca aaagcgatgc gcaggacctg gtgcaggaca
     1141 cggtactcaa agcctaccgc ttcttccacc gctaccaacg cggcaccaat ctcaaggcgt
     1201 ggctcttcaa aattatgacc aacctgtaca tcaacgccta ccgggagaag gcccggaagc
     1261 cgcaggaagt gacgatagaa gatgtggagg agctgtacct ctaccaccgg ttgaccgaga
     1321 cccgccggcc gggtgaggcg aacccggagg aaactttctt cggccagctg tacgaagacg
     1381 aggtccgccg ggtgctggat gaattgcccg ccgattaccg gctggtggtg gtgctggcgt
     1441 ttgtggaaga gttttcgtac gaggagatcg cgcagatcac ccggctgcgg ctggggacgg
     1501 tgaagtcgcg gcttttccgc gggcggaaac tgttgcagaa aaaactgtgg gactacgccg
     1561 tgcgcgccgg ctatttgaag aggaaacgcg aagcatgaac tgcgctcagg cactgtccta
     1621 tctctacgag tacgtggacc ggcacctgcc ggcgcctctg cggaccgagg tgcaggcgca
     1681 catcgccatc tgccggggct gcacccgccg cttcgggatt gaagaaagcc tgcgggcggt
     1741 ggtggggcgc agcggcagag tggtagcaga aatagcggga ctgaaagtag cactgcgtca
     1801 aaagctggcc gctgtgaggg gaaaaggcta agcgacgtac ggcagtggtc cctcgcgccc
     1861 cccggtgcgt agggatcaaa accgggctga cgcaagcata ttcgctccat ttcttaatga
     1921 gtctgtcctt cccccaattt tttttaaccc cctttgagtt ttttctttct caggcgcaaa
     1981 cccgcgttga caaatcgttt ccgggcttta tacttcaagg ccagaagccg gtggtgtccg
     2041 caggagtcga tcgtgctcac cagcaccaga acgatggcgt aggaaaaagt tgccccgcag
     2101 tatgaggaat cgcaaagagg ccttggtcgt tttcgtcaga gcgccgcact acggcgaact
     2161 gaaagcgcgc ttggcccagg aactggcccc cgccgaggtg gagagtctct accgcgcctt
     2221 cgtcggtgac acggtcatgc tctgctctcg agtgaacgac gcgcagctgg tggtgggatt
     2281 cacgccgccg gaggcgcggc gggaagtgac cggtttcgtc gaagaggcgc tcctggcagc
     2341 caatcaccag aagaagccgg tgcccgggcc tttggttttc tccgagcaga ccggggcaaa
     2401 cttaggggaa aggctgcgcg ccatgttcgc gttgctcttc cggcaggggt accacgaggt
     2461 ggtgatcatc ggcgccgaca gccccaccgt gccgccggag acgctgcgga aggcctttgt
     2521 gctgttgaaa aagaagccgg tggttttggg ccccacgctg gacggcggct actacctcgt
     2581 cggcctcgcc cggccggctc cggaaatctt cgacagaatt gcctggggca ccgaccaggt
     2641 ctacccgcag acggtggaaa aattgaagtc cttggggctg gaatgggaag agctgccggt
     2701 gtggtacgac gtggatacgg tagatgatct cgaattcttg gtgcgcgaca tcaaccagtt
     2761 gcgtctggcc ggtgacgaag aatcgctggc agcgaccgaa gtggttttgg aaaagattct
     2821 gaacaagtct taacagccct cccttcacac cgtgaaatta gaagcgctca cttggcagga
     2881 ctttaaacgt ctcgttccgt cgaagattga taccgccctt ttaccggtag gtaccatcga
     2941 agcccacggc gtggcaacgc tcgccaccga taatctcatt ccggaaactt tggcccagcg
     3001 gatggcggac aaagtcaaag ccctcattct gcccactgtt ccctacggta tcacccgttc
     3061 acttttcggc tacccgggct ccttcaccat ttcgccccag aattttaccg cgtacctcgt
     3121 cgatattttt cgctccatcg ccacccagaa atttcgccgg ctggtggtgc tcaacggcca
     3181 cggcgggaat aacgacgcgc tgaaacaagc ggcgctggac atcaaccata ccaccggcct
     3241 caagatcgcg gtcatccact ggtggaccat ctgtcagaaa gtctgccgcg aagtatacgg
     3301 cgaagccggc gggcacgccg cgttggatga aaccgccttt gtcctggcgg tcaagccgga
     3361 gtatgtgaag aagaatcagt acaaaaaaga aatggcctat ttgatgacag aaggtggggc
     3421 ggacatttat ccgaatcccg gctcgatcat tctctacgaa cccgggcagg ggtatctgaa
     3481 ttttgaccca gagaaggcgc aacaatacgt tgaaggatgc gtgaaagaag taacgcggtt
     3541 cgtattagat atttttgtcc ggtggaagaa aatctgagtt ttcatagcat ctgactgcgg
     3601 aaagaattac cagctcccta aactacaggt caaaatgagg aaaacagatg taacaaatat
     3661 tattatggaa taccgagagg caacgcgtca tatttggaat acctattttg taggaaaagt
     3721 gaaatcct
//
LOCUS       DBNGZA010000067         2834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2875920, whole genome shotgun sequence.
ACCESSION   DBNGZA010000067 DBNGZA010000000
VERSION     DBNGZA010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2875920"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC16FU_01385"
     CDS             <1..693
                     /locus_tag="AC16FU_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR2993063.1"
                     /translation="LDRFATFNDFFIREIDLSWRSVDLRPGICIAPVDGRVLAYRRVA
                     RERTFRIKRSSFNLLRFLQNPALADEFNNGSMIISRLSLSDYHHFHFPDSGIPGEAVA
                     IEGQYYAGGPYALGRLVPFYTDNFRMLTRFESNHFGPVLLVEIGAFTVGSIRQCYRIG
                     ARVLKGAHKGFFELGGSTVVLLFRQGAVELDDDLCANTESEMETYVRLGESIGRATSN
                     ISNRIGPDGVQP"
     gene            690..1838
                     /locus_tag="AC16FU_01390"
     CDS             690..1838
                     /locus_tag="AC16FU_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HIR2993064.1"
                     /translation="MNGIRYSQCWEDPRLLAHALEIGPEDDVVSIASGGDNTLALLLG
                     NPRSLIAIDSNPAQIHLLELKIRALEKLEYLDFVGFLGARPCQKRERLYLHVRSSLSD
                     PARKFWDARGSDLARGVIHCGKLERYFSLFRRFILPVIHRPQTISKLLALPSLEEQRT
                     FHSREWNNRRWRWFFRLFFSRFVFSRLGRETAFFNYAATKDVGEELLRRTGRGLCEVP
                     LQDNFFLEYVLTGKYGNLETASPYLCKSNFEFLKKNAGRIRLVIGNLEDYLQKLSPGS
                     VSKLNCSDIFEYVSFEAHGRMLTQLVRVCRPNARLAYWTLLVPRPIPTHLSGRLRLEG
                     ALSERLAALDRAFFYDSFNVAQIIAPAVLCQLKVTDENASHQMPKTRF"
     gene            1930..>2834
                     /locus_tag="AC16FU_01395"
     CDS             1930..>2834
                     /locus_tag="AC16FU_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993065.1"
                     /translation="MSAQQVGATHKKRGVQALPKLLPSGYALRILRSQPLRLGLTIGG
                     IALCLVMMLFLLGVYRGVAEGSVDYIRCNSADLWVLQQNAWNILRGSSLLTRRHGSML
                     AEIPAVKSVSPVLLLLSTIRKQDRIATLFLAGYDPRAKWGGPPSLAEGRSVANDREIV
                     LDQAFAAKFGFKLGDTVLVQNFPLRVVGISTGTNALVIQYAFVTLPRAQALIGFTNIV
                     TCYLVELKANEIRRQAAGAIKRTLPGVEVYDQATFLQNNIREMQTGFLPFLFTIATLG
                     VVVLTAILSLLLSINILERRKDFAVL"
BASE COUNT          561 a          811 c          783 g          679 t
ORIGIN      
        1 ctcgacaggt ttgccacctt taacgatttc ttcatccggg aaattgacct ctcctggcgt
       61 tctgtggatc tccggcccgg tatctgcatc gctccggttg acggccgggt gctggcctat
      121 cgtcgcgtgg cgcgggaacg gacttttcgc atcaaacgct ccagcttcaa tctgcttcgt
      181 tttctccaaa acccggcgct ggcggacgaa ttcaacaatg gttcaatgat tatctcccgt
      241 ctttcgcttt ccgattacca tcatttccac tttcccgatt ccggcatacc gggagaagcg
      301 gtggcgattg aaggccagta ctacgccggc ggcccctacg cactgggccg tttggtacct
      361 ttctacaccg ataatttccg catgctaacg cgctttgagt ccaaccattt tgggccagtg
      421 ttgttagtcg aaatcggagc gttcacggtc ggctccatcc gccaatgtta ccggatcggc
      481 gctcgcgtcc tcaagggggc ccacaagggg ttttttgagc tgggcggttc caccgtggtt
      541 ttgctctttc gccaaggagc cgtcgaactg gatgacgatc tctgcgccaa cacggaaagc
      601 gagatggaga cgtatgttcg cctgggagaa agcatcggac gggccaccag taacataagt
      661 aaccgcatcg gcccggatgg agttcagccg tgaacgggat tcgctactcc cagtgctggg
      721 aagacccgcg ccttttggcc cacgccttgg agattgggcc ggaagacgat gttgtttcca
      781 tcgcttccgg cggggataac acgttggcgc tcttgctggg gaacccccgc tcgctgatcg
      841 ccatcgactc caacccagcc cagattcatt tgctggagtt aaagatccgc gccctggaaa
      901 agctggagta ccttgatttc gtcggttttt tgggcgcccg cccctgtcag aaaagagaac
      961 gattatacct tcacgtccgc tcttcgctaa gcgacccggc ccggaaattc tgggatgccc
     1021 gcgggtccga tttggccaga ggggtcattc actgcggaaa actggaaagg tatttttcat
     1081 tgtttcgacg cttcattctg cccgtcatcc atcggccgca aaccatctcc aagcttttgg
     1141 cccttccatc ccttgaggaa cagcgcacct ttcattctcg ggaatggaac aaccgccgct
     1201 ggcggtggtt tttccggctc ttttttagcc ggtttgtatt cagtcgcctg ggtcgggaga
     1261 cggccttttt caattatgcg gccaccaaag atgtgggcga ggagcttctg cggcgaaccg
     1321 gacgcggcct gtgcgaagtt cctctgcagg acaatttttt cctggagtac gtgttgaccg
     1381 gcaagtacgg caacttggag acggcctctc cttacctctg caagagcaac ttcgaatttc
     1441 tcaagaaaaa cgcgggccgc attcgcctgg taattggcaa cctggaggat tatttacaga
     1501 agctttcgcc gggcagtgtc tccaagctta attgctccga catttttgaa tacgtttcct
     1561 tcgaagcaca tggccggatg ctgacccagc tggtgcgggt ctgccggcca aacgcccggc
     1621 tggcctactg gacgctattg gttccccgtc ccatccccac gcacctatct ggccggctcc
     1681 ggttggaagg ggcgctttca gaacggctgg cagctttgga cagggcgttt ttttacgaca
     1741 gctttaacgt cgcgcaaatc atcgccccgg ctgtcttgtg ccagctcaag gtcacggatg
     1801 agaacgcttc acaccagatg cccaaaacga gattttgacc aagccttgac aattttgtga
     1861 caaccgtacg gtgaataata agtacaatga agcgtgacca acatggaaaa gacggccaaa
     1921 caagttgaga tgtccgcgca gcaggtcggg gcgacgcaca agaagcgggg cgttcaagca
     1981 ttgccaaagc ttctccccag cggctatgcc ttgagaattt tacgaagcca gccgctgcgc
     2041 ttgggtctca ccatcggcgg gattgctttg tgcttggtga tgatgctttt tcttttgggc
     2101 gtttatcgcg gggtcgccga aggatccgtg gattacatcc gctgcaattc cgccgatctc
     2161 tgggtgcttc agcaaaacgc ctggaacatt ctgcgtggtt cgtctctgct caccaggagg
     2221 cacgggagca tgctggcaga aatacccgcc gtaaagtcgg tctcgccggt gcttttactg
     2281 ctctccacca tccgcaaaca ggatcggatt gccacgctct ttctggccgg ctacgacccc
     2341 cgcgcgaaat ggggcgggcc tccgagcctg gccgagggac gatcggttgc gaatgaccgc
     2401 gaaattgttc tggatcaggc cttcgccgca aagtttggct ttaaactggg agacaccgtg
     2461 ctggttcaaa atttccccct gcgggtggtc ggaatcagca ccggcacgaa cgccttggtc
     2521 attcagtatg cctttgtgac cttgccccga gcccaggcgc tcatcgggtt caccaatatc
     2581 gtcacctgct acttagtcga gctgaaagcg aatgaaatcc ggcggcaggc ggccggggcc
     2641 ataaagcgga cgctgcccgg ggtggaggta tacgaccagg cgacgttcct gcaaaacaac
     2701 attcgagaga tgcaaaccgg atttttgcct ttcttgttca cgattgccac gcttggtgtc
     2761 gtggtattga cggccatttt gagcctgctt ctctccatca acatcctgga gcgacgcaag
     2821 gactttgccg tgct
//
LOCUS       DBNGZA010000068         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_755207, whole genome shotgun sequence.
ACCESSION   DBNGZA010000068 DBNGZA010000000
VERSION     DBNGZA010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_755207"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC16FU_01400"
     CDS             <1..940
                     /locus_tag="AC16FU_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR2993066.1"
                     /translation="QSLIPDEIYLPASLEEEDLVRAWLEKKKGAKVAFVYPQRGEKAK
                     LLEMAEQNAKLSLDELLLQKHRAEKKVPEAVTLLKKDLFLDVLPRRIVAFDISNLGTS
                     DPVGSAVYFEDARPKKSEYRHFQIKTVVDQQDDFAMMREVVNRYFSRLIEEQRAFPDL
                     VLIDGGKGQLSSALEALTDLNVTSQPIIGLAKRLEEVILSGAPESLTIPKSSPSLRLL
                     QQIRDEAHRFAITYHRKKRTQRTIKSELDDIKGIGPARRQALLSQFGSVEQIKAADLE
                     ALKQVPGLGERAAQAVHEFFHPSVPVVAEQPIISKA"
     gene            943..1614
                     /locus_tag="AC16FU_01405"
     CDS             943..1614
                     /locus_tag="AC16FU_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2993067.1"
                     /translation="MCSIAPLRESNGLIFEPLVVGPLEVNCYILAERPSGPAVVIDPG
                     ADSERILAVINRHHFYVEKIVLTHAHGDHIGAVSELKRKTGAKILIHPGDAEMLTSAE
                     KNLSLWAGLTVTAPAADQFLNEGDEITIGAVTLKVLHTPGHSPGGISLVGPDFVFTGD
                     LLFAGSVGRTDFPGADAPTLMRSIKEKILPLGDHFTIYPGHGDSSTIGEEKRTNFFLN
                     QRYFV"
     gene            complement(1616..>2362)
                     /locus_tag="AC16FU_01410"
     CDS             complement(1616..>2362)
                     /locus_tag="AC16FU_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993068.1"
                     /translation="KGFSVTALVKGEWAKQREKILWFHSPTDIDDRKRQGIYRGFGFE
                     NGGELRLKTGWVFRLLFGGNYSEGDSATSVVQQATRTYLSDSLDIYVFRSRVNGTHIV
                     RSGLVGVGMQKEVTASTRVLAGVRFQVMAQRLDFHYTFFDSVSRFRNDTLIFQNGSES
                     KRQFFSKPWSDLLTVPLALETNLSPNVEARFGTVWIHGFDRSQAALTTSLQYTLGLGF
                     HFGDLSIDALASNSLTASLQQWRVSFEYRL"
BASE COUNT          571 a          652 c          624 g          515 t
ORIGIN      
        1 ccagtcgctc attccggacg agatttacct gccggcttcg ctcgaggagg aagatttggt
       61 gcgcgcctgg ctggaaaaaa agaaaggggc caaagttgcc ttcgtctacc cccagcgggg
      121 cgagaaggcc aagttgttgg agatggccga gcagaatgcg aaactttcat tagacgaact
      181 cctcctccag aagcaccggg ccgagaaaaa agttccggag gcggtgaccc tcttgaagaa
      241 agacctcttt ctggatgtcc tcccgcgccg catcgtcgcc ttcgacatct ccaacctcgg
      301 cacgagcgac ccggtcggct ctgccgttta tttcgaggac gcccggccca aaaagtcgga
      361 gtaccggcac ttccagatta aaaccgttgt ggaccagcag gacgattttg ccatgatgcg
      421 cgaggtggtg aaccgctact tcagccggtt gattgaggaa cagcgggctt tccccgattt
      481 ggttttaatt gatggcggga agggacaact ctcaagtgcg ctcgaagccc tgaccgatct
      541 gaacgtcacc agccagccga taatcggctt agccaagcgg ctggaggaag tgattctctc
      601 cggcgccccg gaatccctca ccattccgaa gtcctcgccc tctctcagac ttcttcagca
      661 gatccgggat gaggcccacc gctttgccat tacgtatcac cgcaagaagc ggacgcaacg
      721 gacgattaaa tccgaactgg acgacatcaa aggcatcggc cccgctcggc gtcaggcgct
      781 cctttcccaa tttggctcgg tggagcaaat caaagccgct gacctggagg cgctcaaaca
      841 ggtgcccggt ttgggcgagc gggcggcaca ggcggtgcat gagtttttcc atccctcagt
      901 cccggtagtg gctgagcaac cgataatctc aaaagcgtaa gtatgtgcag tatagccccg
      961 ctgcgtgaat cgaatggttt gatttttgaa ccgctggtgg tcgggccctt agaagttaac
     1021 tgttacattt tagccgagcg gccctctggc ccggcggtgg tgatcgaccc cggcgcggac
     1081 tccgaaagaa ttctcgccgt catcaaccgc caccacttct atgtggagaa aatcgttttg
     1141 actcacgccc acggcgacca catcggcgcg gtatcggaat tgaaaagaaa aaccggcgcc
     1201 aagattttga ttcatcccgg cgacgccgag atgctcacta gcgccgagaa aaatctctcc
     1261 ctctgggccg gtctgacggt aaccgcgcca gccgccgatc aatttttgaa tgaaggcgat
     1321 gaaatcacca tcggggcggt gaccttaaaa gtgctccaca cgccgggcca ttcgccgggc
     1381 ggcatttcgc tcgtggggcc ggactttgtc tttacggggg atttgctctt cgccggttcg
     1441 gtggggcgga ctgatttccc cggcgccgac gcgccgactc tgatgcgctc gataaaggag
     1501 aagattctgc ccttaggtga tcacttcacc atctacccgg ggcacgggga ctcctccacc
     1561 atcggagaag agaagcgtac gaatttcttt ttaaatcaga gatactttgt gtgacctaaa
     1621 ggcggtactc aaaacttacg cgccattgtt gcaaagatgc agtgagacta ttgcttgcca
     1681 gagcatcgat acttaagtcc ccaaaatgaa aacccagccc aagagtgtac tggagagagg
     1741 tagttaatgc ggcctgtgag cgatcaaaac cgtgaatcca aacagtacca aaacgtgctt
     1801 caacattggg tgatagattg gtttcgagcg ccagaggaac cgtcaggaga tcggaccacg
     1861 gcttagagaa aaattgacgt ttggattcac tgccattctg gaagataaga gtatcgtttc
     1921 tgaagcgcga aacggaatcg aaaaatgtat agtgaaagtc aagccgttgt gccatgactt
     1981 gaaaacgcac gccagccagg actctcgtcg aagcagtaac ttccttctgc attcccaccc
     2041 ctaccaagcc ggatcgaaca atgtgcgttc cgtttacacg agatctaaaa acatagatgt
     2101 caagagaatc tgacaaatat gttcgggtgg cctgttgaac cacggaggtg gctgagtcac
     2161 cttcactgta attgccacca aacagcagac ggaataccca accagttttt aatctcaatt
     2221 cacccccgtt ttcgaatcca aaccctcggt atataccttg gcgctttcgg tcatcgatgt
     2281 cagtaggcga gtggaaccaa aggatttttt ctctctgctt cgcccattct cctttcacca
     2341 gagcagtaac cgagaaccct tt
//
LOCUS       DBNGZA010000069         4581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_331582, whole genome shotgun sequence.
ACCESSION   DBNGZA010000069 DBNGZA010000000
VERSION     DBNGZA010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_331582"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16FU_01415"
     CDS             complement(<1..352)
                     /locus_tag="AC16FU_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR2993069.1"
                     /translation="MLKKMKTIRVDLGARSYSIHLKARGLADLARLVQREFPESKIAV
                     ITDRNVARLYSRSLMQVFSGRKKQTVFIKLPSGEKTKTLAACQNIYTQLLRRRFDRGD
                     LVIAFGGGVVGDIAG"
     gene            complement(346..915)
                     /locus_tag="AC16FU_01420"
     CDS             complement(346..915)
                     /locus_tag="AC16FU_01420"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR2993070.1"
                     /translation="MRRENIPPSRSFVNIYVTGFMGTGKTVVGRRLARKLNRHFVDLD
                     SEIVRTTRRSIPHIFSEDGERAFRRLEDKVFRRFAVRKKSVVALGGGTLLNRQNMLLA
                     RKTGVIVCLEASLEVILQRVGNAGERPLLNLPGNLPEREQRRMALARIRAMLARRRSV
                     YQQSDFFVNTSRRSVAQVAAAVQSVLEKC"
     gene            complement(908..1360)
                     /locus_tag="AC16FU_01425"
     CDS             complement(908..1360)
                     /locus_tag="AC16FU_01425"
                     /inference="COORDINATES: protein motif:HMM:NF019292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR2993071.1"
                     /translation="MFETSGFLSALFWGALKLLGIVFVIVVPLMVLLEIVQRRRLFDR
                     LIAVVAPVMRFWGFEREAIFPLLAGLSFGVAYGAGVLLQESRENRLSGRQAFLVCTFL
                     GMSHAVFEDTLLFVAVGANGWAVVGVRLLTALVVTFVVAHYVVKVQRA"
     gene            complement(1353..1841)
                     /locus_tag="AC16FU_01430"
     CDS             complement(1353..1841)
                     /locus_tag="AC16FU_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR2993072.1"
                     /translation="MSAAGASAIPLEKSETWPALLTDGLKRGLSTFWFLCKIMVPVYA
                     VVTFLKFTPVLKAIASFCAPAMRFFALPGEAALALVMGNAVNLYAAIAVMITIHLEPA
                     QITLLAVMLGISHSQVMETTIMNKMKINGWAVMALRMAMSLLVGFILGRIALWVGVTL
                     RV"
     gene            complement(1838..2743)
                     /locus_tag="AC16FU_01435"
     CDS             complement(1838..2743)
                     /locus_tag="AC16FU_01435"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR2993073.1"
                     /translation="MPSANRQNLNLVGIIGYPLDHSLSPLLHNTAFRSLRLPWHYQKF
                     EVKPKELANCLAGVRAMGMRGLSVTIPHKEKVIRFLDRVTGAAAKIGAVNTIVNENGK
                     LVGYNTDYLGFLSPLVRLLKPAKPLSVALLGAGGAARAVLFALFSHFRVWETLIFNRN
                     QKRAAALARWAGRVARQEMQIHILRLLDASEEMRLADMDVIVNATPLGLYPKIKQMPV
                     TSADVFRRGQIVYDLNYNPAETRLLREARRQKARTINGLEMLLGQGREAFRLWTGREM
                     PVHQVRTSLTTHLNHHPGPHLADEE"
     gene            complement(2748..4109)
                     /gene="aroA"
                     /locus_tag="AC16FU_01440"
     CDS             complement(2748..4109)
                     /gene="aroA"
                     /locus_tag="AC16FU_01440"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002110455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR2993074.1"
                     /translation="MSPVQLGTQNEVLIRPAKSLSGVITVPGDKSISHRAALLLALAD
                     GTGTIENFSPAADCRSTLACLKKLGVEIKETKENITIAGRGFGGLRRPEAALDCGNSG
                     TSLRLLAGIVSFCDFSARLDGDASLRTRPMQRVVEPLSRMGARFDAPTGTAPLTVHGG
                     ALSGIEYRLPVASAQVKSAVLLAGLHAKGETTVIETAPTRDHTERMLEFLGVTLTAVP
                     ERDESTAEVDFRKKPKTTGQRITVKPIEKIAPKTLFIPGDVSSAAYFIAAALLVRRSE
                     VIIQDVGLNPTRTAFLRLLKQMGANLAIEKTGERNGEPFGTVTVKSSTLSPRKISGEA
                     VAGLIDELPLLALLATQSEGTTVIRDAQELKVKESDRIATVAENLRRMGAKVGILDDG
                     WVIEGKSELDGAEIDPQGDHRIAMTFAVAGLVAHGQALISEAGSVAVSYPNFFETLEA
                     LRR"
     gene            complement(4106..>4581)
                     /locus_tag="AC16FU_01445"
     CDS             complement(4106..>4581)
                     /locus_tag="AC16FU_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR2993075.1"
                     /translation="IQIGARNMQNFSLLKRVGKARKPVLLKRGMAATLEELLMAAEYI
                     MVEGNRDIILCERGVRTFADHTRNTLDLSAVPYVKRMSHLPIIVDPSHGTGRREKVLP
                     MSRAAVAVGCDGLIVEVHHDPGHALSDGPESIYPSQFEQLMQEISQIAAVIGRSV"
BASE COUNT          961 a         1405 c         1271 g          944 t
ORIGIN      
        1 atccggcaat atctcccacc acccccccgc cgaaggcgat gaccaggtca ccccggtcaa
       61 aacgccgccg taatagttgc gtgtaaatat tctggcaggc tgcgagcgtc ttcgtttttt
      121 ctccagatgg gagttttatg aataccgtct gcttttttct tcccgagaac acctgcatca
      181 gcgaccggga atataggcgt gctacatttc tgtcggttat aacggcgatt ttgctttcgg
      241 gaaattcccg ctgcaccaat cgcgccaggt cggccaggcc gcgggctttg aggtggattg
      301 aatacgaacg tgcccccaga tctacccgga tcgttttcat ttttttcaac atttttccag
      361 tacgctttgt accgctgctg caacctgtgc caccgaccgc cgtgatgtat tcacgaaaaa
      421 atctgactgt tggtagacgg agcgacgccg agccagcatt gctcggatac gcgccagagc
      481 cattctccgc tgttcccgct ccgggagatt ccccggcaag ttcaaaaggg gacgctcacc
      541 agcattcccg actcgctgta ggatcacctc taggctcgcc tcaaggcaaa ctatgacgcc
      601 ggtctttctg gccaaaagca tattctggcg gttgagcagg gtcccgcccc ccagtgccac
      661 gacggatttt tttctaacgg cgaacctccg gaaaacttta tcctccaagc ggcgaaaagc
      721 ccgttctcca tcctcgctga aaatgtgcgg aattgaccgg cgcgtcgtgc gcactatttc
      781 cgaatcaaga tcgacaaaat gtcggttgag ttttctcgcc aaccgcctcc ccaccaccgt
      841 cttccctgtc cccatgaagc ccgttacgta gatgttcaca aaacttcgag aggggggaat
      901 gttctcccta cgcacgctgg accttgacga catagtgcgc cacgacgaag gtcacaacca
      961 aagccgttaa aagacgcacg cccacgacgg cccagccgtt ggcccccacc gccacgaaga
     1021 gcagcgtatc ctcgaacacg gcatggctca tccccagaaa ggtgcagact aaaaaggcct
     1081 gccgtccgct caggcggttc tcgcgcgatt cctgcaggag cacgccggca ccgtaggcga
     1141 cgccgaacga caaccccgct aagagcggga aaatcgcctc tcgctcgaag ccccagaagc
     1201 gcatgaccgg cgcgacgacg gcaatcaagc ggtcgaagag ccgtcggcgc tggactattt
     1261 cgagcaagac catcagcggc accacgatga cgaacacgat gcccagaagt ttcagtgctc
     1321 cccagaaaag cgccgagaga aaaccggagg tttcaaacac gcagtgtcac tccgacccag
     1381 agggcaatcc gccccagtat gaatccgacc agaagcgaca tcgccatgcg cagcgccatc
     1441 accgcccagc cgtttatttt cattttgttc ataatcgtcg tttccatcac ctgcgagtgc
     1501 gaaatcccca gcatcaccgc cagaagggtg atttgggccg gctcgaggtg aatggtgatc
     1561 atcaccgcaa tcgccgcgta gaggttgacc gcattcccca tcactagagc cagggccgct
     1621 tctccgggca aagcaaagaa gcgcatggcg ggggcgcaga aggaagcgat ggccttcagg
     1681 accggcgtga atttcaaaaa ggtgacgacc gcgtaaaccg gaaccataat tttgcagaga
     1741 aaccaaaaag ttgaaaggcc gcgtttgagg ccgtcggtca gaagcgccgg ccaggtttcg
     1801 cttttctcca gaggtatggc ggaagcgccg gctgcactca ttcttcgtcc gccaggtgcg
     1861 gtccggggtg gtggttcagg tgcgttgtga gagaggttct aacctggtgc accggcattt
     1921 ctcggcccgt ccagaggcga aaggcctccc gcccctggcc cagaagcatt tcgagtccat
     1981 tgatggtcct ggctttttgc cggcgggctt cccggaggag ccgcgtctct gccggattgt
     2041 aattcaggtc gtagacaatt tgcccccggc ggaaaacgtc tgccgaagtg accggcatct
     2101 gtttgatttt cgggtataaa cccagtggcg tggcgttgac gattacgtcc atatcggcca
     2161 gacgcatttc ttcggatgcg tccagaagcc gcagaatatg aatctgcatt tcctgacgtg
     2221 caactcgccc ggcccaacgc gcgagcgccg cggcacgttt ctgatttcgg ttgaaaatca
     2281 aagtttccca cacccgaaaa tgggaaaaaa gggcaaagag aaccgcgcgc gccgctccgc
     2341 ctgcaccgag gagggcgacc gagagaggtt tggcgggttt cagcaaacgg acgagcggtg
     2401 agaggaatcc aagatagtca gtattatagc cgaccagttt gccattctcg ttcacaatcg
     2461 tgttaaccgc accgattttg gccgccgcgc cggtgacgcg gtctaagaac cggattacct
     2521 tttccttatg cggaatggtg acgctcaaac cccgcatccc catggcccgt actccggcca
     2581 aacagttcgc cagttccttc ggcttcacct cgaatttctg atagtgccag ggcagccgca
     2641 atgaccggaa ggcagtgttg tgcagaagcg gcgaaagcga atggtcgagg gggtagccga
     2701 tgatgccgac caaattcagg ttctgtctgt ttgcgctcgg cattggttca gcgacgcagg
     2761 gcttccagcg tttcaaaaaa attcgggtag gaaaccgcaa ccgacccggc ctcggaaatc
     2821 aacgcctgcc cgtgggccac gagcccggcc accgcaaagg tcatggcgat gcggtggtct
     2881 ccctgcggat caatttccgc gccgtctaat tcgcttttgc cttcaatcac ccagccgtcg
     2941 tccagaatgc ccactttcgc ccccatccgg cgcagattct cggccacggt tgcgatccgg
     3001 tccgactcct ttaccttcaa ctcctgcgcg tcgcggatca ccgtggttcc ttcgctctgg
     3061 gtcgccaaaa gtgccaaaag cggaagttcg tcgattaatc cggcaacggc ctcgccggaa
     3121 attttcctgg gggacaaggt acttgatttt acggtaaccg taccgaacgg ctcaccgttc
     3181 cgctccccgg ttttctcgat ggcgaggttg gcccccatct gtttgagcag ccggagaaag
     3241 gccgttcgag tcggattgag acccacgtct tggataatga cttccgagcg ccgcaccaga
     3301 agtgcggccg cgatgaagta cgcagcggaa gaaacatccc cgggaatgaa aagcgttttc
     3361 ggtgcgattt tttctatcgg tttgacggtg atcctctgcc ccgtagtctt gggctttttc
     3421 cgaaagtcaa cctcagctgt tgactcgtcc cgctcgggca cagccgtcag cgtaaccccc
     3481 aaaaactcca gcattctttc ggtatggtcg cgggtcggcg ccgtttcgat gacagtcgtc
     3541 tcgcctttcg cgtgcaggcc ggccaggaga accgccgatt tcacctgcgc cgaagccact
     3601 ggcaggcggt actcaatccc gctcagggcg ccgccgtgca cggtcaaagg cgcggtgccg
     3661 gtgggagcgt caaaccgcgc gcccatacgg gagagcggtt ccaccacccg ttgcattggc
     3721 cgggtgcgca aggaggcgtc accatccagc ctggctgaaa aatcgcagaa acttactatc
     3781 ccggccaaaa gccggagcga cgttccggag ttgccgcagt ccagcgccgc ttccggcctg
     3841 cggaggccgc cgaatccccg gccggcaatc gttatgttct cttttgtctc ttttatttcg
     3901 acacccaatt ttttcaaaca ggccagggtg ctccggcagt cggcggccgg agaaaaattc
     3961 tctatggtcc cggttccatc agccaatgcc agaaggagcg ccgcccggtg ggaaatcgat
     4021 ttgtcgcccg gaactgtaat gacgcccgag agacttttcg ccggtcggat caagacttcg
     4081 ttctgtgtgc cgagctgaac cggactcata ccgatctccc gatcaccgcg gcaatctggc
     4141 tgatttcctg catcaactgc tcgaattggg agggataaat cgactctggc ccgtcggaga
     4201 gggcgtgtcc cggatcgtgg tggacttcga caatcagccc gtcgcacccc accgccactg
     4261 ccgcccgcga catcggcagc actttctcgc ggcgcccagt tccgtgggag ggatcgacga
     4321 taatcggcag atggctcatc cgcttgacgt agggcactgc cgaaagatcg agcgtgttgc
     4381 gggtgtggtc ggcaaaggtg cgcactcccc gctcgcagag gatgatgtcg cggttcccct
     4441 ccaccatgat gtactctgcc gccatcagga gctcttctaa ggttgccgcc atgccgcgct
     4501 tgagcagcac cggcttccgc gccttgccga cccgcttcag aagtgaaaaa ttctgcatgt
     4561 tgcgcgcgcc gatctggatg a
//
LOCUS       DBNGZA010000070         1710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2134592, whole genome shotgun sequence.
ACCESSION   DBNGZA010000070 DBNGZA010000000
VERSION     DBNGZA010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2134592"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="AC16FU_01450"
     CDS             complement(<1..1177)
                     /locus_tag="AC16FU_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993076.1"
                     /translation="MKKLQLFWIAATLSTGWCDYAVALPQNPLWGQDYLAYDGHIAAF
                     AADYTTDGSIYAVVVPAASPDSMVLLKSDNGGACWSEIGGRLVASGDSISLVRLATIE
                     YGTVKGLLVFSLTRLGGGVLRATRFSTAGVFNAQSTVLAGILGDTLERFSVSPNLERG
                     DTLVLAFGTKKGSVGVTRSVNAGATWGAYRLLAVGSTGLPDLAYGSKGAYFFACYNSS
                     TGEIQTFTSRDYGLTWTGPTAVARDTLRLALDLQVMAEHDANVDSTTLWVFTNRLETD
                     ALLCGAGISFCFQLDLFFNASLDAGVKWSADSNLTSTPCCCNAGTTVGYHEILPRLAV
                     SKVKPTRVSDLTYILVGGACSGVSAVEHLSSTVDAPDIWTAPQAVNVSGITEAIPAQI
                     "
     gene            complement(1222..>1710)
                     /locus_tag="AC16FU_01455"
     CDS             complement(1222..>1710)
                     /locus_tag="AC16FU_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2993077.1"
                     /translation="VIYTGLVDIRTGETLAVDTSLKRIMPGVLALQVVPPDALIFIDG
                     VAVSLVNGRAELPAGGHTLKATRTGYKPYDGKFSISSGEQISLTINLEPASQPATPAL
                     AFNETPKPSKPLFKKPVFWGILGAAVIGGVVVAAGGGGKGGGTGGGTTALPPPPPPPG
                     KR"
BASE COUNT          342 a          530 c          516 g          322 t
ORIGIN      
        1 caatttgtgc gggaattgct tccgtaatgc cgctgacgtt caccgcctgg ggcgccgtcc
       61 agatatcggg tgcatccacg gtggaggaga ggtgctctac tgccgaaacc ccgctgcacg
      121 cgccccccac taagatgtaa gtgaggtcgc tgacgcgcgt cggcttcact ttggagacgg
      181 ccaggcgcgg gaggatttcg tggtacccca ccgtcgtacc ggcgttgcag cagcagggtg
      241 tactggtcag attggaatcg gcgctccact tgacacccgc atccaggctc gcattgaaga
      301 acaaatccag ctgaaaacaa aaagagatgc ccgccccgca gaggagcgcg tcggtttcca
      361 gccggttggt aaaaacccac agcgtggtgg aatcgacgtt ggcgtcgtgc tcggccataa
      421 cctgcaggtc cagcgcgagc cgcaaggtat cgcgggcgac cgctgttggt ccggtccagg
      481 tcaaaccgta atctcgggag gtaaacgttt gaatttcacc ggttgacgaa ttgtagcagg
      541 cgaaaaagta ggcccctttg gatccatagg cgaggtccgg gaggccggtg gagccgacag
      601 cgagcaggcg gtaggcgccc caggtggctc ccgcgttgac gctgcgcgtc acccccacgc
      661 tccccttctt cgtcccaaat gccaagacca gcgtgtcgcc ccgttccaga tttggcgaca
      721 ccgagaaacg ctccagggta tctccgagaa ttcccgccag gacggtgctc tgcgcgttga
      781 atacccccgc ggtggaaaaa cgggtggcgc gcagcactcc gccccccagc cgggtcagac
      841 tgaagaccaa gagccctttc acagtgccgt actcgatggt ggccagccgc accaggctga
      901 tcgaatcgcc cgacgccacc agccgcccgc cgatctccga ccagcaggcg ccgccgttgt
      961 ccgatttcaa gaggaccatc gaatcgggag aagccgccgg caccacgacc gcatatattg
     1021 aaccgtcggt ggtgtaatcg gcggcaaaag cggcaatgtg gccgtcgtaa gccaggtagt
     1081 cctgtcccca gagcggattt tgcggaagag ccacggcata atcacaccag ccggttgaca
     1141 atgtggcggc aatccaaaac agttgcaatt tcttcacggt tccctccctt ggctggcttt
     1201 gcgtttcaca gtagtaactg cttaacgttt ccccggcggc ggtggcggcg gcggtaaagc
     1261 ggtcgtacct ccaccggtcc ccccgccttt ccctccgcca cccgcggcca ctaccacgcc
     1321 gccgatcact gccgctccca gaatgcccca gaagaccggt tttttgaaaa gcggcttgga
     1381 aggtttcggc gtttcgttga acgctaaagc cggagtggcc ggttggctgg ccggctcgag
     1441 atttatggtc aggctgattt gttcgcccga actgatgctg aatttgccgt catacggctt
     1501 gtagcccgta cgggttgctt tgagcgtatg tccgccggcg gggagctcgg cccggccgtt
     1561 gaccagagag accgccacac cgtcaatgaa aatcagggca tcgggaggaa caacctgcaa
     1621 cgccagaact cccggcatga tccttttgag actggtatcg acggcgagtg tctcgccggt
     1681 tcggatgtcg accagaccgg tgtagatcac
//
LOCUS       DBNGZA010000071         4426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2664176, whole genome shotgun sequence.
ACCESSION   DBNGZA010000071 DBNGZA010000000
VERSION     DBNGZA010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2664176"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="AC16FU_01460"
     CDS             complement(<1..708)
                     /locus_tag="AC16FU_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993078.1"
                     /translation="MGRKFIGAIFLFFIMSLAWTNDGWAQVVPSVTESLIVNGVAVPK
                     TPGSSFSITLSLTNQRRISALSGRLVFDPNIITPEVSGADVVMQRVGRGDTFAFFGAS
                     THQPGAVIFGMFTDLFDTVVHSLPAGRGPVCQINFTVVASIDTTICVRFQDNPADTED
                     VKNQLSDEQGFLILPSLGPSSVQVGAGSATGGCGPVQPPPDTLRNDPPIINPIAAQTI
                     EQGSILSFVVTASDPDGG"
     gene            complement(738..1493)
                     /locus_tag="AC16FU_01465"
     CDS             complement(738..1493)
                     /locus_tag="AC16FU_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993079.1"
                     /translation="MKIDSVPGFPGALVSIPIELTNDRPIIGLNIRLHYDVLAESLHS
                     VTPGERVADWEFFDATPDTGSGEIHIVGYADFFSPVDTIVMPAGSGVVGYLNFKVYDP
                     SLFVGSFTDVVFLYPESDTNVMRDSTGGLIGHNEITYLNGGIDLRTTDVSDWRENRPN
                     QFALEQNFPNPFNPQTKIDFYLPASEHARLVVYDLLGRTVRQLVDENLPAGAHAALWD
                     GRDSGGHPAASGVYFYRLVAGPFHQTKKMILVK"
     gene            complement(2127..2759)
                     /locus_tag="AC16FU_01470"
     CDS             complement(2127..2759)
                     /locus_tag="AC16FU_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2993080.1"
                     /translation="MNPAEGAVSITLSSAGPALTLSANSASDLGGLYLKFKVSGTVGT
                     LALAKAAEGMTMKTNLDGNVLSVLIYSETKGQRIEANAGALFSMAVAGSAELIESGAA
                     DYYGIALPTLTKVAALPTSFGLSQNYPNPFNGRTSFVLALPVASDYRVTVYNVAGQVV
                     RTFEGSAPAGNRTITWDGTDRNGTAVSSGVYFYKAVAGNYSATLKMLYLK"
     gene            complement(3714..4319)
                     /locus_tag="AC16FU_01475"
                     /pseudo
     CDS             complement(3714..4319)
                     /locus_tag="AC16FU_01475"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a         1275 c         1236 g          910 t
ORIGIN      
        1 accgccgtcc ggatcggaag cggttactac aaaggacaaa atggaacctt gttctatggt
       61 ctgggcggca atgggattaa tgatcggcgg gtcattcctc aaagtatcag gaggaggttg
      121 caccggcccg cagcctcccg tcgcactgcc ggctccaact tgcacgctgg atgggcccag
      181 cgagggaaga atcaaaaatc cctgctcgtc ggaaagctgg ttttttacgt cctcggtatc
      241 cgccggattg tcctgaaaac gcacgcaaat ggtggtatcg atggaagcca ccacggtaaa
      301 atttatttga cagaccggac ctctcccggc gggaagggag tgcaccaccg tatcaaaaag
      361 gtcggtaaac attccaaaaa ttacggctcc cggctggtgc gtggaggcgc caaagaaagc
      421 aaacgtatcc cccctgccga cccgctgcat cacgacgtcc gcaccggaca cttccggcgt
      481 tatgatgttc gggtcaaaca caagccgtcc ggaaagcgcc gaaatccgcc tttgattggt
      541 caatgaaagc gttatcgaaa acgaactccc gggggtcttg ggcacggcca cgccgttgac
      601 gattagcgat tccgttacgg agggcacgac ctgcgcccag ccgtcgtttg tccaggccag
      661 actcataatg aaaaaaagga aaatcgcccc aataaatttt cttcccatct ttcttccttc
      721 ccttgacagt cgtcttctca tttcaccaga atcatttttt tggtttgatg gaacggcccc
      781 gccaccagcc ggtaaaaata aacgccggaa gcggccggat gcccgccgct gtcgcggcca
      841 tcccagagag ccgcgtgcgc cccggccggc aaattttcat ccaccagttg gcgcaccgtg
      901 cggccgagca ggtcatagac aaccagacgg gcatgttccg atgcgggcag gtaaaagtcg
      961 attttcgtct gggggttgaa ggggttggga aagttctgct ccagtgcaaa ttggttcggc
     1021 cgattttccc tccaatcgga aacgtccgta gtccgcaggt caatgccgcc gttgaggtag
     1081 gtaatctcgt tatgcccaat cagccccccg gttgagtcgc gcatcacgtt ggtgtcactt
     1141 tccgggtaaa gaaaaaccac gtccgtaaag gagccgacaa aaagggatgg gtcatatacc
     1201 ttaaaattca aatagcccac aactccgctg ccggccggca tgacgatggt gtctacggga
     1261 gaaaaaaagt cggcataccc cacgatatga atttcgccgc tgccggtatc gggggtcgcg
     1321 tcaaaaaatt cccagtcggc cacccgttct cccggtgtga cggaatgcag cgattccgcc
     1381 aggacatcgt aatgcagtcg tatatttaag ccaatgatgg ggcggtcgtt ggtcagttca
     1441 atgggaatgc tgaccaaagc tccgggaaat ccgggaaccg agtcaatttt catcacgttt
     1501 gcgccgccac ttccaaaact cgctcccccc caaacgcttg tggctgcgct ccaacccaaa
     1561 gaaaccccga gaatcaagaa ccctccttta accatcgttt tttaggggta catgacccca
     1621 acgccaagat tccgcaaacc ccataccgct cccgcagcaa aagcgggaat atgggggctg
     1681 gcccaagcct ggatcgagcc gcttaacttg ttttgtttta attacttccg gcccggtcgc
     1741 gcacggcaac caaaaaccgg taccaagcgg tagcgagatt tacgaaggta gagaactcac
     1801 ctctgtggtt tgaatgccca gcttatgcaa tcccctgcaa cccaatcttt ttccggctct
     1861 ctctgcgtaa cagaagctcg gccggctttg gaaggtcccg accgcccctg caggacctgc
     1921 cagaaataaa aaaatcagtt caaatttttg ctgcaaactg cctcttccca tatataatgt
     1981 cgcgcgcact ttctctcctt tatgaaaact acctgttggc tattttgctc ggttattcct
     2041 cctttctttt ctccctcttc cggtactatg atttcgggag tcccgtcccc cgaaggggac
     2101 gggagctccc aaaatcgaac tgccaattac ttcagataca acatcttcaa agtggcgctg
     2161 tagttgccag ccaccgcctt gtagaagtag acacccgaag aaacagccgt tccgttgcgg
     2221 tccgtgccat cccaagtgat ggtccggttg cccgccggag ccgaaccttc aaaggtcctc
     2281 acgacttgac cggccacgtt gtaaaccgtg accctgtagt cggaagccac cggcaaagcc
     2341 agaacaaagc tggttctgcc gttgaagggg ttcggatagt tctgcgacag gccgaacgaa
     2401 gtcggcaggg ccgccacctt ggttaaagtc ggcagcgcaa tgccgtagta atcggccgct
     2461 ccggactcga tgagctcggc cgagccggcc acggccatgg agaacagggc gcccgcattc
     2521 gcttcgattc tctgaccctt ggtctcggag taaatcagaa ccgaaagcac gtttccatcc
     2581 aggttggtct tcatggtcat gccttcagcg gctttcgcca aagccagcgt tccgacggtg
     2641 ccgctgacct tgaatttcag gtacaggccg cccagatcgg aagcggaatt ggccgaaagc
     2701 gtcaaagcgg gaccggccga agacagcgta atggaaaccg cgccttccgc cggattcatc
     2761 ctcggcagcg ggttttcgtc gccggtgatg atgcgaatca aggacaccaa atccgccacg
     2821 gaaagaacca gtccgtcgcc gttgatgtcg gaggccgcaa tctgcccctg acggcgctgc
     2881 ggatcggggg acaaaacgcc tgggccgtag atgaagtagt tctcatacaa aacggcgtcg
     2941 gcaatttcgt tcttcagtcc gttcaggttc aaatcgccgc gatcgtcgat ttcttccggc
     3001 gtcggcaggc agatttgccc gttgcagaag atgatgtttt tcttcggtgt cggtttgtcc
     3061 gggccgcccg agaaggcgtc gcagttggcg acgttgttcg ggaagctggt ggccagatcg
     3121 atgagagcgc cggaggcgct gtagagcgaa tcgaccacgt acagcacgtt accggtcgaa
     3181 tccgacacgg tgttgtcggt gcagtcatac cagaaccaag aaatggcgca gcactgtccc
     3241 gccagggtcc ggtcgttgga ggtgcggaac ttcagcacca cccattcccc ttggttcgtg
     3301 tggtcataaa gcggatggga agcgccgttg ttaacgtcgg cgatcgccac cacccgaatc
     3361 aagcaggaag gacacccggc gccgcagttg ccgttgccaa tggcgccgta gcggtaggtg
     3421 aagaactccc accgatgact caccaaaaag gcgcccttgc gggccgacag gaactgcaag
     3481 cagctgcagt cataggacaa taggaatgag aaaccgccca catccttgcc gttggccagg
     3541 agcggatcgg tggcatcatc cacgttccga atggaaactt ccacatccgt gccctgataa
     3601 actccgcgcg gacccagctt gtcaatacag atgcggaacg gcccgcccac caccacgcgg
     3661 atggtaaagc tgcattgagc cgtttgctgg cagttgtcga caacgtgtac gatcacggtg
     3721 tgcgaaccga cgtccgccac ggtaggcgtc cagctgatgg ccccggtgga agtgaccacc
     3781 aaaccaaccg gattgctggg atctccggag aaggtatagg gtggaacccc gctcgaagcc
     3841 gacgcgttac cattgtaagt cgaaccggcc tgcacctgcg ccgatggcgg gcagatggcg
     3901 ttaggcggtc cggggttgaa atgcgcggta aaggtgtagg gtatatcatt cgggccgcaa
     3961 tgctcgatga tcgttaccgg atagtctccc ccgcactggt cgcacaggtt aaaagcacca
     4021 tctgtagtga tggttgcacc cggcggtgaa ggttgaccca tggtaaagtt atccgaacac
     4081 ggggaagctg cgttgaaatt gtagttggcg cagccggtgc tgggatctgc gtttacgatg
     4141 ttccccggag gaggattgct caccggcggc tggctgacgg tgataactcc ctttacgaag
     4201 ttcaccggca cgtcagccac gaactggcca tggtcccgga gaatggtgaa accggttttc
     4261 tgggccagcg cccccgacgt atccacgtcg acggttccca acggcgcatc gctgctcatg
     4321 tacttgaact cccagatggc cgaacgggtc aggttgatcg gttcggcatc ccgaaccggg
     4381 ttcgacccag tgggggagaa ggtggaagcc accaggaaat aatcgg
//
LOCUS       DBNGZA010000072         5719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_225597, whole genome shotgun sequence.
ACCESSION   DBNGZA010000072 DBNGZA010000000
VERSION     DBNGZA010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_225597"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..883)
                     /locus_tag="AC16FU_01480"
     CDS             complement(47..883)
                     /locus_tag="AC16FU_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR2993081.1"
                     /translation="MLKLPFMLQALAGCLLLAVTLSYFGIHVVLRRIVFVDLALAQVS
                     SIGVAVAMLVEGNVTLYALAFTLLGAAVFSIRIKERRIPQEAAIGFVYAVASAVAILL
                     ISKSPHGEADVLKILFGNILAVNKKQLLEMAFVFGGVGILHFLLHKKIFAISQAEAHH
                     EFSARHLLLWDLLFYLTLGLVIAFAIRSAGVLLVFSFLIAPGIFAIGLGARSLGALYA
                     LAAGLGVINSFTGLYLSYRYDLPTGAALIATFGAFIVLAGAVRLVFLRRNKTENEVQP
                     GG"
     gene            complement(889..1323)
                     /locus_tag="AC16FU_01485"
     CDS             complement(889..1323)
                     /locus_tag="AC16FU_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR2993082.1"
                     /translation="MPLFFEANLKTFNETLTQQEARWVRLMEPFQGAKVITYHNSWPY
                     FVRRFGLNVVGYVEPKPGIPPPPSHVAELIDQVKREGIKLIIMEPYFSEKIPQLVASQ
                     TGCRVLVLPPSVGGVKGVDDYAALLDYDLNQIVSALKGQGTP"
     gene            complement(1307..1828)
                     /locus_tag="AC16FU_01490"
     CDS             complement(1307..1828)
                     /locus_tag="AC16FU_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR2993083.1"
                     /translation="MQKKKICRWLAAVGIPLLLASGVTGAQKIKVVTTTTDLKALAEA
                     VGKDKVEVTAIARGYEDPHFVEAKPSHMLKLQKTDLLVKVGLDLEIGWLPVLLDGARN
                     PKIMPGAPGHVDASKSIAVLEVPTGKVERSQGDIHALGNPHYWLDPENAKASAASIAD
                     GLKAIDPSDAAFF"
     gene            complement(1872..3059)
                     /locus_tag="AC16FU_01495"
     CDS             complement(1872..3059)
                     /locus_tag="AC16FU_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-regulated TonB-dependent outer membrane
                     receptor"
                     /protein_id="HIR2993084.1"
                     /translation="MRLFGWTFLFVFLAVETGSAQQPAPSPRGGASLNPDISLDGLFS
                     AGYFSKIPNLQFGGHDPKNRGFTAENIELTLGSVVDPYFRADAHIVLLIEDGETVVEV
                     EETYLTTLALPHHLQLLAGQFFTRFGRLNPTHPHTWDWADQPVVNSRFFGPDGVRNPG
                     MQLSYLFPLPFYLELAGSVQNANGETAVSFLSKEGEEIAGRILPKREVHSLGDLLYLG
                     RARASWDLSEAATLVLGSSALLGPNASGPRARTNIFGGDLYFKWRPARASFGWPFFTV
                     QTEALWRNYQAGEFITPVSETLAATTLRDGGFYTQAAYGFTRRWVAGLRFDLARGNDP
                     TDPQRDHRWRGALDLTFYPSEFSKWRLQYDHDVAQHRSDRPIHALFLQWEFLIGAHGA
                     HKF"
     gene            complement(3134..3379)
                     /locus_tag="AC16FU_01500"
     CDS             complement(3134..3379)
                     /locus_tag="AC16FU_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993085.1"
                     /translation="MPLAITFLPLLHHHAGPDGRSAATHCVACSFSHNLFGGQVTLFA
                     LVFSCLVLWFFSLQPALHFSLFSPLGTPTRAPPAFRF"
     gene            complement(3498..4175)
                     /locus_tag="AC16FU_01505"
     CDS             complement(3498..4175)
                     /locus_tag="AC16FU_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6946 family protein"
                     /protein_id="HIR2993086.1"
                     /translation="MSKILIPTKSAEDWKQFLAEPDKHWKRGYSARAIAYCWQEADGL
                     PDDVKKVLMQEPAFEGIEILFAIPEHQVMLNSGTGPSQNDVWVIARTRTSLVSIAVEG
                     KVSESFGPTMAEWSINPSEGKLERLKYLCSVLSLKAKPDDDIRYQFLHRTTSAIIEAN
                     RFQASHAVMLVHSFSLTDEGLTDYKRFLSLFNVNGEADSIIHAGRVSGVALYFAWVRG
                     DKQYLER"
     gene            complement(4807..5256)
                     /locus_tag="AC16FU_01510"
     CDS             complement(4807..5256)
                     /locus_tag="AC16FU_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993087.1"
                     /translation="MKRTRAIGGNLRRIFSFLALSTAFLGSGCTKEPGQVSYEVVQEW
                     SLPNGGHGRVIVIDKKHRNEADLKKLAEQLKRSTAQDRNAFVFIYDDKKAAESRKAAL
                     DNRLSGQELVYHDTHLVGSYFKNAGTRYQALTITLQGVMGPAKEIKL"
     gene            complement(5401..>5719)
                     /locus_tag="AC16FU_01515"
     CDS             complement(5401..>5719)
                     /locus_tag="AC16FU_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993088.1"
                     /translation="AIDFDAGKDASAYEFTYSNDHQGFAPSIEYELPGMGHTVDATVE
                     TRLLPPTENGPHVLQFFDMEEGEGPDEWWAFTCKNYRCDYQIYKSEPPSEFIKLWKTA
                     KKL"
BASE COUNT         1295 a         1665 c         1519 g         1240 t
ORIGIN      
        1 atccggctga agcagttccg aacttccaca ggttgactcc ctaaaattat ccccccggct
       61 gcacctcgtt ttcggtttta ttccgccgga gaaatacgag tcgtaccgct ccggcaagaa
      121 cgatgaaggc cccgaaggtg gcaatgagcg ccgccccggt cggcagatcg taccggtacg
      181 aaaggtaaag cccggtgaaa ctgtttatga cgccgaggcc cgccgccagt gcgtagagcg
      241 ctcccaaaga ccgcgctccg aggccgatgg caaaaatccc cggcgcaatg aggaacgaaa
      301 aaaccagaag cacccccgcg ctccggatgg caaaggcgat cacgagcccc agggtgaggt
      361 agaagagcaa atcccagagc aagaggtgcc gggcggaaaa ttcgtggtgc gcctccgcct
      421 gcgaaatcgc gaaaatcttt ttgtggagca ggaagtgcaa aatccccacg ccgccgaaga
      481 cgaaagccat ttccagcagt tgctttttgt tcaccgccag aatgttcccg aagaggattt
      541 tgagcacgtc ggcctcgccg tgcggcgatt tggaaatcag aagaatggcc acggcggagg
      601 ccaccgcgta aacgaaaccg atcgccgcct cctgcggaat ccgccgctcc ttgatccgga
      661 ttgagaacac cgccgcgccc agaagggtaa aggccaacgc gtagagggtg acgttgccct
      721 ccaccagcat cgccaccgcc acgccgatag aggaaacctg cgccagcgct aaatcgacga
      781 agacgatccg ccgcaagacg acatggatgc cgaagtagga gagcgtgacc gccagaagca
      841 ggcagccggc caacgcctgg agcatgaagg ggagtttcag catctcaatc atggcgtccc
      901 ctgccctttc agcgcgctca cgatctggtt gaggtcgtag tccaggagcg ctgcgtagtc
      961 atccactcct ttcaccccgc ccaccgaagg cggcaggacg agcacccggc agccggtctg
     1021 gctcgccacc aactgtggaa ttttctcgga gaaatacggc tccatgatga tcagtttgat
     1081 tccctcgcgt ttcacctggt cgattaactc cgccacgtgc gatggcggcg gcggaatgcc
     1141 cggtttgggc tcgacgtacc ccaccacgtt caggccgaaa cgccgcacaa agtagggcca
     1201 ggaattatgg taggtgatca ctttggcgcc ctggaacggc tccatcagcc ttacccagcg
     1261 cgcttcttgc tgcgtcagcg tttcgttgaa cgttttgagg ttggcctcaa aaaaaagcgg
     1321 catcagaggg atcaatcgcc tttaacccgt ccgcaatgct ggccgcgctc gctttggcgt
     1381 tttccggatc gagccagtag tgcggattgc ccagagcgtg gatgtcgccc tgcgaacgtt
     1441 ccactttgcc cgtgggcact tcgagcacgg caatggactt agacgcatcc acgtgccccg
     1501 gcgctccggg catgattttc ggattgcgcg cgccgtccaa aagcaccggc agccagccga
     1561 tttcgagatc gagccccact ttcaccaaga ggtcggtctt ctggagtttg agcatgtgcg
     1621 acggcttggc ctccacaaag tgcgggtctt cgtacccccg cgcaatggcg gtgacttcga
     1681 ctttgtcctt ccccaccgct tccgccaggg ctttgagatc ggtggtcgtc gtcacgactt
     1741 ttattttctg tgcgccggtg accccgctcg ctaacagcag aggaatgccg accgccgcca
     1801 accaccggca aatcttcttt ttttgcattt gtcttttcct ttccctctgt tttgtgtgcc
     1861 gtctaccgtg tttagaactt atgcgccccg tgcgccccga tcagaaactc ccactgcaaa
     1921 aacagcgcgt gaatgggacg atcactccgg tgctgagcga cgtcatggtc gtactgcaag
     1981 cgccatttcg agaactcgct cgggtagaag gtgaggtcga gcgcaccgcg ccagcggtga
     2041 tcgcgctgcg ggtcggtggg atcgttgccg cgcgccaggt cgaaccgaag tcccgccacc
     2101 caccgccggg tgaagccgta ggccgcctgt gtgtagaaac ccccatcgcg gagcgtcgtc
     2161 gccgccagcg tttcggatac cggggtgatg aattcacctg cctggtagtt gcgccagagt
     2221 gcttcggtct gcacggtgaa aaagggccac ccgaaactgg cgcgtgccgg ccgccacttg
     2281 aagtacaggt cgccgccgaa aatattcgtc cgcgccctcg gcccggaagc gttcggcccc
     2341 aaaagtgccg acgaacctaa aaccagagtc gccgcctcac tcaagtccca cgaggcccgg
     2401 gcgcgtccga ggtacaaaag gtcgccgagg gagtgaactt cccgcttggg taaaatcctc
     2461 ccggcaatct cttccccctc tttactcaga aaactgactg ccgtctcccc attcgcattc
     2521 tgaacgctgc ccgccagttc cagatagaac ggcagcggga aaaggtacga caactgcatt
     2581 ccgggattgc gcaccccgtc cgggccgaag aagcggctgt tgaccaccgg ctggtcggcc
     2641 cagtcccagg tgtgcgggtg ggtcgggttc agacgcccga aacgtgtgaa gaactgcccg
     2701 gccaaaagct gcaggtgatg cggtaacgca agcgttgtca ggtaggtctc ttccacttcg
     2761 acgaccgttt ccccgtcttc gatcagaagg acgatatggg cgtccgcccg gaagtaggga
     2821 tcgaccacgc tccccaaggt caactcgata ttctctgccg tgaatccccg gtttttggga
     2881 tcgtgcccgc cgaactggag gttcggaatc ttcgagaagt agccggcgct gaataagccg
     2941 tccagggaaa tgtccggatt caaactcgcc ccgccccgcg gggaaggagc cggctgctgt
     3001 gctgatcccg tctctactgc cagaaaaacg aaaaggaacg tccacccaaa aagtcgcatc
     3061 ttgccaccgc ccttcgcttg agctaacccg accgattcgg cctatgccga atcagtatca
     3121 cgaactgatc aggttaaaag cgaaaggcag gcggtgcgcg ggtgggagtt ccgagtggag
     3181 aaaataggga aaagtgaagt gccggttgaa gcgagaagaa ccagagaacc aggcagctga
     3241 agactaaagc gaagagggta acctgcccgc cgaaaagatt gtgtgagaag gaacaggcga
     3301 cacagtgggt ggccgccgac cggccgtcgg gcccggcatg gtggtgcagg agcggcaaaa
     3361 aggtgattgc cagcggcaac cacaaaagaa aggcgattcg gaaaaaaaga ttcgatctcc
     3421 ccatctctgg atttattata cgcaatagag ttgaaaatcg ctacgccagc cgctggagca
     3481 acttgttaga ccattcttta tctctcaaga tattgtttgt ctccacgaac ccacgcaaag
     3541 tataaagcaa ctcctgaaac ccgcccagca tgaataatac tatccgcttc tccgtttacg
     3601 ttgaacaatg aaagaaaacg tttgtagtct gtcaatcctt catcagtcag gctgaatgaa
     3661 tgaacaagca tcactgcatg actcgcttga aatctgtttg cttcaatgat tgctgaagtt
     3721 gtacgatgta aaaactggta tctaatgtcg tcatctggtt ttgctttaag actaagaaca
     3781 gagcataaat atttcagtct ttccagtttg ccttctgatg ggttaatact ccactcagcc
     3841 attgttggcc caaatgactc tgaaaccttg ccctctactg ctatggagac aagacttgtt
     3901 cttgttcttg ctataaccca tacatcattc tgtgatggtc ctgttccact gttcaacatc
     3961 acttggtgtt ctggaatggc aaaaagtatt tcgattcctt caaaggcagg ctcctgcatc
     4021 aaaacctttt tcacgtcatc aggtaagccg tccgcctctt gccaacaata tgcaattgcg
     4081 cgggcagagt aaccgcgctt ccaatgttta tccggctcag ccaaaaactg cttccaatct
     4141 tctgctgact tggtagggat caatatcttg ctcataatct gtcatcctcc tttctttgta
     4201 ggttcttttt tcttccgctc caaggccgcg gccaaaccta gcgaaattac ctcatgaagc
     4261 agctgaatgc ggccagccaa tgatgaacga tcagcagatt tccggccctc tgacaaccca
     4321 cgaaggctaa caagctctct aaactgttca atgtcctctc ggagcagcac tgggttgtgg
     4381 agaagctcct cgtggcactc gtagcagagc aatgctgact cgccctcaac accccagggg
     4441 cacttctcaa agataccccc tgtctgcgtg ccgcgacgat tctgcgagcg tccaaagaag
     4501 cgctcagcaa cgaagtgatg cttcgttgcg tggctacgcc cttcaaccgt aggacgagcg
     4561 taagtgccgc tccggcggtg cagagcacaa ccgcagattt cgcaattctc ggtcatggcc
     4621 aatgacagtt aaaaatgatt atacagtctg cataataccg caaaggcagt cgctttcctt
     4681 acgcataata cctattcatg tctggaggag tataggtaaa agataccgca ggtgctacgc
     4741 ctgctccggc gggccaaacc aatctctgct agtcggggca aacacttgtc agagatgcca
     4801 gcgggcttaa agttttatct ccttagcagg tcccattacg ccttgcagcg ttatggtaag
     4861 cgcctgatac cgggtgccgg catttttgaa gtaggagccg acgaggtggg tgtcgtggta
     4921 gaccagctcc tgcccgctca gccgattgtc cagtgccgcc ttcctcgatt ccgccgcttt
     4981 tttgtcgtcg tagatgaaga cgaaggcatt gcggtcctga gccgtgctcc gcttgagctg
     5041 ctcggccagc tttttcaggt cggcttcgtt gcggtgcttt ttgtcgatga cgatcacccg
     5101 tccgtggccg ccattgggta acgaccactc ctgcaccacc tcataactga cttgtcccgg
     5161 ctctttggtg cagcccgatc ccagaaaagc ggtcgaaagc gccaggaacg aaaaaatccg
     5221 ccgcagattg ccgccgatgg cgcgtgtcct tttcatggaa ttacccctcc cctccggaat
     5281 ggagctttcc tcgcgcaaaa ttaaaccgga aaacgagtcc tgtcctctca tcttcggatg
     5341 gagtatatgt aaaagccacc gcaggtgcta tgcccatctg tcgtcaggat taggtaggcc
     5401 ctataacttc ttggccgtct tccacaattt gatgaactca cttggtggtt cactcttgta
     5461 tatctgataa tcgcaacgat agttcttaca tgtgaaggcc caccattcat ccggcccttc
     5521 accctcctcc atatcgaaaa actgaagaac gtgcgggccg ttctccgtag gcggcaatag
     5581 ccgggtttct acagtggcgt ccactgtatg ccccatgccc ggcaattcat attcgatgga
     5641 gggagcaaat ccttgatggt cgtttgagta agtgaattca tatgcactgg catcttttcc
     5701 agcatcgaaa tcaatagct
//
LOCUS       DBNGZA010000073         3794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1604413, whole genome shotgun sequence.
ACCESSION   DBNGZA010000073 DBNGZA010000000
VERSION     DBNGZA010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1604413"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC16FU_01520"
     CDS             complement(<1..338)
                     /locus_tag="AC16FU_01520"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743973.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR2993089.1"
                     /translation="MASVKALKSQRYRWPDKGGHFGKFGGMFVPETLVTPLLELWDGY
                     WKARRDREFQSELRGLLAHYAGRPTPLYFAERLSGKLGLRIYLKREDLTHTGAHKINN
                     TVGQILLAKRL"
     gene            complement(325..942)
                     /locus_tag="AC16FU_01525"
                     /pseudo
     CDS             complement(325..942)
                     /locus_tag="AC16FU_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012900.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            complement(936..1745)
                     /gene="trpC"
                     /locus_tag="AC16FU_01530"
     CDS             complement(936..1745)
                     /gene="trpC"
                     /locus_tag="AC16FU_01530"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR2993090.1"
                     /translation="MSILDDIVAVKEREVAEAKTSVPLDCLMKRIQSKVGRPFREALT
                     NGDALSVIAEVKKASPSSGLLQPDYDPVAIAQGYKQNGAAALSVLAEVHYFQGAPEHL
                     QQVSENVPLPVLWKDFVIDEYQIYQAKAAGADCVLLIAALLPGRRLATFLTDVRRLGL
                     EALIEVHAESELQQATDAGADLLGVNNRNLRTLAVDVRTSFRLAERLPQEVVAVSESG
                     IETPETLVELSRLGYRAFLIGTYFLRQANPGRALADLLAKARTLRQKAAVC"
     gene            complement(1745..3091)
                     /locus_tag="AC16FU_01535"
     CDS             complement(1745..3091)
                     /locus_tag="AC16FU_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2993091.1"
                     /translation="MSAAVEQVQEEKLESTPAPEGPPPFFAHPRRWLTDYGTHLALFS
                     RNARLFLFGSFFMGLGFSVFQVLLNLYFKELGFRESTIGDILSVGALGSVLIAGPAAI
                     LLSRRDAKPFLVVTAVLAGLTYSLQATVVQKAGLLAASLGTGMMVSVFRVAAAPFFMR
                     NSTQKERTYLFSVNFGIGTLAGFVGSLLGGWLPAVFSSFDSSIIHSHRWALVSGAGLT
                     LLAAIPFALLRPQAPVSGQSGQKLAWHLLKKKGGLYFKLCFPFLILGLGAGLVIPFLN
                     LYFKTRFQQSSGEIGTFFAILSFATLAGVLTGPVLTRKWGMVRTIVWTQFISIPFMLI
                     LAFTYFLPLAVVAFLFRGALMNLGQPIAVHFTMEQVAPEEHALANSLLMLAWAGAWVV
                     STNLGGKLIARYGFTPPLLVTVGLYIVSSVLYYLYFAHAEKVPKAVEAVPVPEAVG"
     gene            complement(3117..>3794)
                     /gene="trpD"
                     /locus_tag="AC16FU_01540"
     CDS             complement(3117..>3794)
                     /gene="trpD"
                     /locus_tag="AC16FU_01540"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR2993092.1"
                     /translation="SSRCGSADVLEALGVKIEASAPTVNRCLREVGIGFLFAPLFHQA
                     MKHAASPRKELGLRTAFNLLGPLTNPARPRRQMVGVYDPVLTELIASVLQKLGTERAL
                     VCSGMDGLDELSLSGESKITELANGSLRTYSLKPEEVGLARVPKAKLSGGEAGENAAI
                     LSRILAGENGPARDVVLLNAGAAIYMAGEAKTIAAGIEVAKKSIDSGAAQKKLERLVA
                     VSQKSVS"
BASE COUNT          850 a         1148 c         1055 g          741 t
ORIGIN      
        1 agccgcttcg ctaacaatat ctgaccaaca gtattgttga tcttgtgcgc gccggtgtgg
       61 gtcaggtctt cgcgcttcag gtaaattcgc aggcccagtt tgcctgagag acgttccgca
      121 aagtagagag gcgtcggacg accggcgtag tgcgccagca aaccccgcag ttcgctctga
      181 aattcccgat cgcgcctcgc tttccagtag ccatcccaga gttcgagcaa aggcgttacc
      241 agggtctccg gcacgaacat cccgccgaat ttcccgaaat gcccgccttt atcgggccag
      301 cggtagcgct gggatttcaa agccttaacg gaggccattg attaccttaa agatcgctcg
      361 caatttttcc gggtctttct tgcccggctt cgcttcaacg ctggaggaaa aatcaaccac
      421 cgcgggctcg accgccgcca ccgcctgcgc gatattgtct tcgcgcaagc caccggacag
      481 aatttaatcg gctttgcctt tcaggtgggc gatccgcttc cagtcaaaac tccttcccgt
      541 gccgccggtc cgcgccgcat cgtacgtatc gaacagaaag taatcgacgt tgtaaacgta
      601 cacgttggga aattcgccgc cgcctatccg gaaggtttta atcaccggga cttggcgcgc
      661 cagccgccgg caggtctgcg gcgtctcgct cccgtgcagc tgcaccatat ccagtcccac
      721 cccgcggacg gttaacaaga tcgttttctc cggtgcatcg acaaaaacac cgactgccag
      781 cgtgaagggc gaaagtttct tgacgattgt actcgccaaa cgcggcgtga ccgctcgtgg
      841 gcttttcggg tagaaaacga agcccaaagc atcagcgccc agggactccg ccagccgggc
      901 gtcttccaga ttcgtaatgc cgcacacttt cactttcagc atacagctgc cttctgccgg
      961 agtgtcctcg ctttcgccaa caaatccgcc agcgcccggc ccggattggc ctggcgcagg
     1021 aagtaagtac cgatcaagaa tgctctgtag ccgagtcggg agagttccac cagcgtttcc
     1081 ggcgtctcga taccgctctc gctcaccgcc acgacctctt ggggcagtcg ttctgccagg
     1141 cgaaatgagg ttctcacatc cactgcgagc gtccgcaaat tgcggttgtt aacgccgagc
     1201 aaatcggcac cggcatcggt ggcttgttgc aattcagact cggcgtgtac ttctatcaat
     1261 gcctccagac ccaatcttcg gacatcggtg aggaaggttg ccaaacgtct ccctgggaga
     1321 agtgcggcaa ttaagaggac gcaatctgcg ccagccgctt tggcctggta aatctggtac
     1381 tcgtcgatga caaaatcttt ccacagaacc gggagcggaa cgttctcgct cacctgctgg
     1441 aggtgctccg gcgctccctg gaagtagtgt acttcggcca aaaccgaaag cgccgccgcg
     1501 ccgttctgtt tgtatccctg agcgatggcc actggatcgt agtcaggttg cagcaggccg
     1561 ctggaaggcg acgctttttt cacttcagcg atgaccgaga gagcgtcacc attcgtcagg
     1621 gcttcccgaa atggccggcc aaccttcgat tgaatccgct tcatgaggca atcgagcggc
     1681 acgctggttt tcgcttccgc cacctcgcgc tctttgactg caacaatgtc atctaaaatt
     1741 gacattaccc caccgcctcg ggtacgggaa cggcttcaac ggctttaggc accttttctg
     1801 catgggcaaa gtagaggtag tagaggacag aagaaacgat gtacagaccg acggtcacca
     1861 gaagcggcgg ggtgaacccg taccgggcaa tcaacttgcc gccgagattg gtgctcacca
     1921 cccaggcccc ggcccaggca agcatcaaaa gactattggc cagcgcatgc tcttccgggg
     1981 cgacctgctc catcgtaaaa tgcacggcta tcggctggcc taaattcatc aacgcgccgc
     2041 ggaaaaggaa cgccaccacc gccaaaggca gaaagtaggt gaacgccaga atcagcataa
     2101 acgggatgga gatgaactgc gtccagacga tggtgcgcac catcccccat ttgcgggtta
     2161 gaaccgggcc ggtgagcacc cccgccagcg tggcgaaaga caggatggca aagaaggtgc
     2221 cgatctcgcc ggaagactgc tggaagcggg ttttaaagta gaggttgaga aaggggatca
     2281 ccaggccggc gcccaatccc aaaatcaaaa acgggaagca gagcttgaag tagagtccgc
     2341 ctttcttttt cagcaaatgc caggccagtt tctgtccgga ttggccactg actggagcct
     2401 gcggccgcaa cagggcaaag ggtatggctg ccagaagtgt caaacccgcg ccgctcacca
     2461 gtgcccagcg gtgtgaatga atgattgaac tgtcaaaaga gctgaatacg gccggcagcc
     2521 agccccccaa tagactcccc acaaaaccgg ccagggtccc gatgccgaag ttcaccgaaa
     2581 agaggtaagt tcgctctttt tgcgtgctgt tgcgcatgaa aaaaggcgcc gccgccaccc
     2641 gaaagaccga gaccatcatc ccggtgccga gactggccgc caaaaggccc gctttttgca
     2701 ccacggtcgc ttgcaaagaa taggtgaggc cggccaaaac cgcggtgacg accaaaaacg
     2761 gcttggcgtc acgccgcgaa agcagaattg ccgccggccc ggcaatcaaa accgaaccga
     2821 gcgctcccac cgaaaggatg tcgccgatgg tgctctcccg aaaaccgagt tccttgaagt
     2881 agaggttcaa aagcacctgg aagaccgaaa accccaaccc catgaaaaat gaaccgaaga
     2941 gaaagagccg cgcgttgcgc gagaagagcg ccaggtgcgt cccgtagtcg gtcagccacc
     3001 gccgcggatg ggcgaagaaa ggcggcggtc cttccggtgc gggagtggat tccagcttct
     3061 cttcctgaac ttgttctacg gctgcgctca caaatttcct cgccggattg atttgattaa
     3121 ctcacgcttt tctggctgac tgctaccagc cgttccaatt tcttctgcgc ggcgccggag
     3181 tcaattgatt ttttcgcgac ctcaatccct gcggcaatcg tctttgcctc tcccgccatg
     3241 taaatggccg ccccggcatt caggagaacc acgtcgcgcg ccggcccgtt ttccccggcc
     3301 aggatgcggg agagaattgc ggcgttctca ccggcctcac caccggaaag tttcgctttc
     3361 gggacgcgcg ccagaccgac ctcttccggt ttcaaggagt aggtgcggag cgagccgttg
     3421 gccagttcgg ttattttcga ttcgccggag agcgagagtt cgtccagacc atccattccg
     3481 gagcaaacca gtgctcgctc ggtgcctaac ttctggagca cggaagcaat cagttcagtg
     3541 aggacggggt cgtaaacacc gaccatctgc cgccggggcc gggccggatt ggtgagcggg
     3601 cctaagagat taaaagcggt gcgtagcccc aattccttcc gggggctggc cgcatgcttc
     3661 atcgcctggt ggaataaagg cgcaaagaga aaaccgatcc ctacctcgcg gagacaacga
     3721 tttaccgttg gggccgacgc ttcaatcttt actcccagag cttccagcac atccgcactg
     3781 ccgcagcgtg aaga
//
LOCUS       DBNGZA010000074         3311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_14631, whole genome shotgun sequence.
ACCESSION   DBNGZA010000074 DBNGZA010000000
VERSION     DBNGZA010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_14631"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="AC16FU_01545"
     CDS             complement(<1..1762)
                     /locus_tag="AC16FU_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2993093.1"
                     /translation="MPKPLIAFFGTLLLLTGLVSLSSQAAEPQVLVTIEPTTAEQIAA
                     ARDLGVVAYQRWERLLLGTIPEKNLPLLRAAGLPVTVLEPQPWSGTYTLVTPRRESTR
                     KAADVAQYGEVLTTFGPGALVKNLGPEPEAELRENFHLVAVTPDPIPLELPTLVAPNP
                     AYFAGATLLDSLVSLVSTESLSTYDNRLVAFQTRYSFADSNYRARDWLVQKFHDFGYT
                     DVVVDTFYYQGRFLNNIICTKPGTAEPDAIVVVGGHFDSVVYGAGDPMIFAPGADDDG
                     TGTVGTLELARVLAQTPLRKTIKFITFNTEEQGLIGSNAVASRFRGQGKDVRLMVNMD
                     MIAYVTDTIPDFDIEMTNQWASFAQLLAAAARTFTPLIPYFTTAAANSDHWPFIQYGY
                     PAVFASEGDFNQPNWHQPTDLPGKLYFPYARDVVKAVLATMYAVSEFPAGVTNLRAAD
                     LGTGDALEVRWNHSRDSLNVQGYKICWGTVSGILDSSKVVSGTTTVDTIRGLTDGQTY
                     FISVTPLPFFTSLTSCVGPQVQAAPHRAPLAPVGIRAAPGWHSATVAWNANHEVDLTG
                     YRLYRFTGAGDTLVIPRGLPE"
     gene            complement(1985..2251)
                     /gene="rpsT"
                     /locus_tag="AC16FU_01550"
     CDS             complement(1985..2251)
                     /gene="rpsT"
                     /locus_tag="AC16FU_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR2993094.1"
                     /translation="MPRHRSPEKALRKSTRENLRNRIWKSKVKTAVKAVEAAPDAATA
                     QAQLRTAVSLLDKAARRKIIHPNAAARVKSHLAKSIRARVTRQP"
     gene            2436..>3311
                     /gene="murJ"
                     /locus_tag="AC16FU_01555"
     CDS             2436..>3311
                     /gene="murJ"
                     /locus_tag="AC16FU_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR2993095.1"
                     /translation="MSTSAPRLDSGPAPLARNAAVVSLATVASRLFGLLREQTFAYLF
                     GAGLATDAFVAAFRIPNLLRDLFAEGVLGVAFVPVFTDSLTKKGKVEAFRVANLCASG
                     LVVAVSAVVIVGIVASPWIVHLIAPGFEKIPGKMELTALLTRIMFPFLLFIALAALWM
                     GMANSLGSFAIPAAAPILFNLGMIVAGFTLGSLFAEPVVAMALGVLVGGFGQWAIQWP
                     VVHRHGFRPAWELSFRDPELRRILVLMVPAVIALASTQVNIFVNTLVASLLPQGSVSY
                     LNYSFRLMHFPLGAFG"
BASE COUNT          625 a          946 c         1011 g          729 t
ORIGIN      
        1 tctcgggaag tccccggggg ataaccagcg tgtcgccggc gccggtgaag cggtagagcc
       61 ggtagccggt caggtcgact tcgtggttgg cgttccaggc gacagttgcg gagtgccagc
      121 caggggcggc gcgaatgccg accggggcca gcggagcccg gtgcggcgcc gcctgcactt
      181 gcggtcccac acagctcgtg agactggtaa agaacggcag cggagtcacg gaaatgaaat
      241 aggtctgtcc atcagtcaat ccccggatgg tatcgacggt agtggtgccg ctgacgactt
      301 tgctgctgtc gagaatgccc gacaccgtac cccagcaaat tttatatcct tgaacgttca
      361 gagagtcacg cgaatgattc caacggacct ccaaagcatc acccgtgccg aggtcggcgg
      421 cacgcaggtt cgtcactcca gccggaaatt cggacaccgc atacatcgtc gccaacaccg
      481 ccttcaccac gtcgcgcgca tacgggaaat acagtttacc cggtaagtcg gtcggctggt
      541 gccaattcgg ctggttaaaa tcgccttccg aagcgaagac ggcgggatag ccgtactgaa
      601 tgaacggcca gtgatcggaa tttgccgccg cggtggtgaa gtaagggatg agcggcgtaa
      661 aagtccgcgc ggctgcggcc agcagttggg caaaggaggc ccactgattg gtcatttcga
      721 tatcgaagtc cggaatggtg tcggtgacgt aagcgatcat atccatattc accatgagcc
      781 ggacgtcttt gccctgccct cggaagcgtg aggcaacggc gttactgccg atcaagccct
      841 gctcttcggt attgaacgta atgaatttga tcgtcttgcg caggggagtt tgggccagca
      901 cccgggccag ttccaaagtt cctaccgtgc cggtaccatc atcatcggct ccgggagcga
      961 aaatcatcgg gtctcctgcc ccgtagacaa ctgagtcgaa gtgtccgccg acgaccacga
     1021 tggcgtccgg ttcggcggtg cccggcttgg tgcaaatgat gttgttcagg aaacggccct
     1081 ggtagtaaaa ggtatcaacg accacgtcgg tgtacccaaa gtcatggaac ttctgcacta
     1141 gccagtctcg cgcgcggtag tttgaatcgg cgaaactgta acgggtttgg aaagccacca
     1201 ggcggttgtc gtacgttgaa agcgactcag tcgaaaccag ggagaccaaa gaatcgagga
     1261 gggtggcgcc ggcaaagtag gccgggttcg gggctaccag cgtcggcaat tccagaggaa
     1321 tgggatcggg ggtgaccgcc accaggtgaa agttctcccg cagctcggcc tcaggttccg
     1381 gccccagatt tttcaccagc gcaccgggcc caaaggtagt gagcacttca ccgtattggg
     1441 ctacgtcggc tgctttcctg gtgctttctc tccggggagt gaccagggta taagtacccg
     1501 accagggttg tggttcaagc acggtaacgg gcaagccagc ggcccggagc agcggcaggt
     1561 tcttttccgg gatggtgccg agcagcagcc gttcccagcg ttgataggca acgacaccca
     1621 gatctcgggc cgccgcgatc tgttccgctg tcgtgggctc gatggtgact aacacctgag
     1681 gttccgccgc ctggctggag agtgaaacca ggccagtgag caagagcagg gttccgaaaa
     1741 aagcaatcag cggtttcggc atgagtttcc tcctggggga aagatagcac gggtgggaga
     1801 agcagtcacg tttctttcac aatcactttt gagaaccgaa gatttcaaac gacgaaattt
     1861 ttcgtcgata aacaaaatac ggagaaggag gtctttttcc tacaagaata atgccattcc
     1921 ggacggaagc actctcttta gggatgcctc gggacgagcg ttgtttccgg tcctgcggtt
     1981 aggactacgg ttgccgggtt acgcgcgctc gtatagattt cgccagatgc gatttgaccc
     2041 gggcggcggc attcgggtgg atgattttcc gacgggcggc tttgtccaga agcgacacgg
     2101 ccgtccgcaa ctgcgcctgc gccgtagccg catcgggggc cgcttccacc gctttcaccg
     2161 ccgttttcac tttcgatttc cagatgcggt tgcgcaggtt ctctcttgtg ctcttccgca
     2221 gtgccttctc gggcgaacga tgtcggggca actcctcctc ctctatttcg ggactcaggc
     2281 atttacttat tctttgggca gtgaatatag ctcttttgcg cgggccgtca aattccgctc
     2341 ctccggattt ggcggcaaac gaactctgca caagcggatg accgggaagt gaacaacagg
     2401 ttcgccgagg cggtttgccg ggtcgtgccg gatccgtgag caccagcgcc cctcggcttg
     2461 actcgggacc agcccctctg gcccgaaatg ccgccgtggt cagtctggcc acggtcgcct
     2521 cgcggctctt cggcctgttg cgtgagcaga cctttgccta cctcttcggg gccggcctgg
     2581 ccaccgacgc ctttgtggcg gccttccgca ttcccaattt gctgcgcgac ctgtttgcgg
     2641 agggcgtcct gggcgtcgcc ttcgtcccgg tctttaccga tagtctgacc aaaaaaggga
     2701 aagtcgaagc cttccgcgtc gcgaatctct gcgccagtgg attggtcgtc gccgtctcgg
     2761 ccgtggtcat cgtgggaata gtcgcttcac cctggattgt ccacctgatt gcacccggat
     2821 tcgagaaaat tcccggaaag atggaattga ccgcgctcct cacgcggatc atgttcccgt
     2881 ttcttctgtt catcgcctta gccgcactct ggatgggaat ggccaactcg ttaggatcat
     2941 tcgcgatacc agcggcggcg ccgattctct ttaacctggg gatgattgtg gcgggtttca
     3001 cgctcgggtc gctctttgcg gagccggtcg tcgccatggc cttaggcgtt ttggtcgggg
     3061 gattcggtca gtgggccatt cagtggcccg tggtgcaccg gcacgggttt cgtcccgcct
     3121 gggagcttag ctttcgcgat ccggaactgc gccgcatcct cgttctgatg gtgccggccg
     3181 tcatcgccct ggcttcgacc caggtgaaca ttttcgtcaa tacgcttgtg gcctcgcttt
     3241 taccccaggg gagcgtttcc tatctcaact acagttttcg gctgatgcat tttcctctgg
     3301 gtgccttcgg c
//
LOCUS       DBNGZA010000075         1468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_651080, whole genome shotgun sequence.
ACCESSION   DBNGZA010000075 DBNGZA010000000
VERSION     DBNGZA010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_651080"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /gene="ruvX"
                     /locus_tag="AC16FU_01560"
     CDS             complement(<1..108)
                     /gene="ruvX"
                     /locus_tag="AC16FU_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018924126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR2993096.1"
                     /translation="MQRILGIDYGRKRVGVAVSDLLGLTAQMLDTLEVES"
     gene            265..1299
                     /locus_tag="AC16FU_01565"
     CDS             265..1299
                     /locus_tag="AC16FU_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR2993097.1"
                     /translation="MRRLGFVLCLLTFSVAGVTAHTVHYDQLLDPVTHEPPIATFSIC
                     AFDPETGDFGVAVQSKFFGVGTVVPWAKAGVGAIATQAWGNTSFGPLGLEMLAHGYTA
                     QQVVDALIAGDDHRHRRQLGVIDSKGNAANWTGDSCLAWAGGIKGRYYTCQGTILTGD
                     SVVKAMARAFEATKGDLAERLMTALEAGQSKGGDSRGMQSAALLVVRDKGGYNGYTDR
                     YVDLRVDDAPNPFKEMRRLLGIQQGLKHLQVAGLKYREGKIKEAIGECREAVKTDPTN
                     ADNYYDLACYLSLDKEKTEALDALRSAMKLNPKLKKLAEGDSDLDNIRNENAFKTILE
                     GHRGTRGLRR"
BASE COUNT          342 a          407 c          408 g          311 t
ORIGIN      
        1 cgattcaacc tccaaggtgt ccagcatctg cgccgttaag cccaaaagat cggagaccgc
       61 tacgcccact cgctttctgc cataatcgat gcctaaaatt ctctgcatag ggatttacag
      121 acgtgccacc gataatttat tctcaaaggc tgcctccgtc aaacgttgcg gccgttttcc
      181 cccgcaggct ttacccttgc catttctctc aaagagggta acttaggcca catgtttgca
      241 acttttccaa caaaggagat ttctatgcgg cgcctgggat ttgtgctgtg tctgttgaca
      301 ttttctgtcg ccggtgtcac ggcacatacc gttcactacg atcaactcct cgacccggtg
      361 actcatgaac cgcccatcgc cactttttcc atttgcgcct tcgatccgga aaccggtgat
      421 tttggcgtgg cggtgcagtc aaaattcttc ggggtcggga cggtcgtccc ctgggccaaa
      481 gccggggtgg gagccatagc cacccaggcc tggggcaata cttcctttgg ccctttgggg
      541 ctggagatgc tggcccacgg ctacaccgcc cagcaggtgg tggatgcact catcgcggga
      601 gatgaccacc gccaccgccg gcaattaggg gtcatcgact cgaaaggcaa tgccgccaac
      661 tggacgggcg attcctgcct ggcctgggcg ggaggcatca aagggcgcta ctacacctgt
      721 caggggacta tccttacggg agacagcgtg gtgaaggcaa tggctcgggc ttttgaagca
      781 accaaaggtg atctggctga acgcctgatg actgctttgg aagcaggcca atccaaaggc
      841 ggcgactcgc gcgggatgca gtcggctgcg ctcttagtcg tgcgcgacaa aggcggctac
      901 aacggctaca ccgaccgcta cgttgatctg cgggtcgatg acgcccccaa ccccttcaaa
      961 gaaatgcgcc gcctgctcgg catccagcag gggttaaaac acctgcaggt ggcgggattg
     1021 aagtaccgcg agggaaaaat caaagaggcg atcggcgagt gtcgagaggc ggtcaaaacc
     1081 gaccccacca acgccgacaa ctattacgac ttagcctgct acctgtcgct cgacaaggaa
     1141 aaaaccgagg cgttagatgc cctgcgctcg gcgatgaaac tgaacccgaa gttgaagaaa
     1201 ctggccgagg gagattccga tctggacaac attcgcaacg agaacgcgtt caaaacaatc
     1261 ctggaaggac atcggggaac gcgcggtttg cgacgttgaa aacagcggct atagagacga
     1321 aaagacttga caaattaatg actttcttat aaatttatca aggactgcct ccccgaggga
     1381 gggtttgaaa tgcgacgggt aatctttctt gtttttttaa ccgcaggagc attggcctcc
     1441 ggcggtttct ttctttgcag caagagga
//
LOCUS       DBNGZA010000076         1867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1498895, whole genome shotgun sequence.
ACCESSION   DBNGZA010000076 DBNGZA010000000
VERSION     DBNGZA010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1498895"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16FU_01570"
     CDS             complement(<1..191)
                     /locus_tag="AC16FU_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993098.1"
                     /translation="MKRVTVLTVGPVFITLLAAFAHAQEAQKPEAVAKSQHVMLTESD
                     LKWTDPAALPAGAKLAVLEG"
     gene            complement(250..1335)
                     /gene="mqnC"
                     /locus_tag="AC16FU_01575"
     CDS             complement(250..1335)
                     /gene="mqnC"
                     /locus_tag="AC16FU_01575"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR2993099.1"
                     /translation="MNSVSLDAIRKKVLAGERITPAEGISLMREDDLLSVGDLAGQVR
                     WRRHPEPVVTFVIDTNPNYTNVCYIDCIFCAFYRHEGDPDAYTLSVDQVLEKVKWAEE
                     QGATTVLLQGGVNPALPLDYYLDIVRRMRQEFPRMHPHFFSAVEIQGMASVSGLSIRE
                     VLLKLKEAGLATLPGGGAEILADRVRMKLGKKKGMSNAWLEVHREAHQLNFKTTATMM
                     FGHIETDAELIEHLERIRALQDETGGFTAFIPWSFKPGNTPLEKRYPHHAGPNRYLRI
                     LAVARIFLDNFPHIQASWFSEGKKTGQVALHFGADDFGGTIFEENVHKCAGFVNTTTT
                     DEVVALIQEAGFRPAQRTTLYEIIKYL"
     gene            complement(1316..>1867)
                     /locus_tag="AC16FU_01580"
     CDS             complement(1316..>1867)
                     /locus_tag="AC16FU_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HIR2993100.1"
                     /translation="GAVKSVVLFSKVPREKLSGRKIGITPETSTSYRLLQLLCRGFWR
                     VAPSAFIRDAAAISSEADVYLWIGNEVLRRNHGRSPEFPYQYDLALEWWRWQQKPFVF
                     ALWAIHRKVTTLERTRLAQVLEGSLDETSGQFEKVAQRLSGVLGTTAELTEYLSNFTY
                     RLGRDEWQGLETFRRLLDEFRIA"
BASE COUNT          382 a          566 c          504 g          415 t
ORIGIN      
        1 ccttccagga cggcgagctt ggcacctgca ggcagcgccg ccgggtcagt ccacttgagg
       61 tcgctttccg tcagcataac atgctgactt ttggcaaccg cttccggctt ctgcgcttct
      121 tgcgcgtgtg caaaagctgc aagcagggtg atgaaaacgg gcccgacggt cagaactgtg
      181 acgcgtttca tttttccctc cttccttact gtttttgtcc gctctctctc ggcgaatgaa
      241 aaaccgccgc tataggtatt tgatgatctc gtacagcgtc gtgcgctggg ccggacggaa
      301 gccggcttct tgaatcaggg ccaccacctc gtccgtcgtg gtggtattca caaaaccggc
      361 gcacttgtgg acgttctctt cgaagatcgt gccgccgaaa tcgtccgctc cgaagtgcag
      421 cgccacctgg ccggtctttt tcccttccga aaaccaggag gcctggatgt gcggaaagtt
      481 gtccagaaaa atgcgcgcga ccgccagaat gcgcagatag cgattcggcc ccgcatggtg
      541 cgggtatctt ttttcaagcg gggtattgcc cggtttgaag ctccagggaa tgaaggcggt
      601 gaagcccccg gtttcatcct gcagcgcccg gattctctct aaatgctcga tgagctccgc
      661 gtcggtttca atgtggccga acatcatggt tgcagtggtt ttgaagttca actggtgcgc
      721 ttcgcggtgg acctccagcc aagcgttgga catccctttc ttcttgccta atttcatccg
      781 cacccgatcg gccaagattt cagccccgcc gccgggcagc gtcgccagtc cggcctcttt
      841 caatttcagc aggacttccc gaatcgataa acccgacacg ctggccatgc cttgaatttc
      901 cactgcggaa aagaagtgcg ggtgcatccg gggaaactct tgccgcatcc gccgcacgat
      961 gtctaaatag tagtccagcg gcaaagccgg attgacgccg ccctgcaaga ggaccgtggt
     1021 ggcaccttgc tcctccgccc atttgacttt ttccagaacc tgatcgactg aaagcgtgta
     1081 agcgtccgga tcaccctcgt gtcggtagaa agcgcagaag atgcaatcaa tataacagac
     1141 gttcgtataa ttcggattcg tatcgatgac gaacgtcacc accggctccg ggtgccgccg
     1201 ccagcgaacc tggccggcca ggtcaccgac gctcaagagg tcatcttccc gcatcaggga
     1261 gattccctct gccggggtga tcctctcccc tgctaatact ttcttacgga tggcatcaag
     1321 cgatacggaa ttcatccagc agtctccgaa aggtttccaa accctgccat tcgtcccgac
     1381 ccaggcggta ggtaaaattg gagagatact ccgtcaattc agccgtcgtg ccgaggaccc
     1441 cgctcaatcg ctgcgcgact ttttcgaact gcccgctggt ttcatccagc gagccttcca
     1501 gcacctgcgc cagccgagtg cgttccaaag tcgtgacctt tcggtggatg gcccagagcg
     1561 caaaaacgaa tggcttttgt tgccaccgcc accactccag cgccagatcg tactggtagg
     1621 gaaactccgg acttcttcca tggttgcggc gtaagacctc attgccaatc cagaggtaga
     1681 cgtcggcttc ggatgaaatt gcagccgcat cccggatgaa ggcgctgggc gccacccgcc
     1741 aaaagccccg gcagaggagc tgcaagagac ggtaggaagt tgacgtctcg ggagtaatgc
     1801 cgatcttccg cccggagagt ttttcccgag gcactttgga aaataacacc acgcttttga
     1861 ccgctcc
//
LOCUS       DBNGZA010000077         2720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_862781, whole genome shotgun sequence.
ACCESSION   DBNGZA010000077 DBNGZA010000000
VERSION     DBNGZA010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_862781"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="AC16FU_01585"
     CDS             <1..650
                     /locus_tag="AC16FU_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HIR2993101.1"
                     /translation="YRDGSKRTQPLVTGLGEPTATVAAPRRRRLPDERKAVTHKFSIA
                     GHEGYITVGLYDDGVPGEIFITMAKEGSTVSGLMDSFATAISLALQYGVPLKTLVEKF
                     SHTRFEPAGFTNNPEIPLARSVMDYIFRWVGKKFLPPEDQPKGQTSATTEENGGTKTA
                     TAHPVGKQTTLFAGEKTDTFENQTDAPACPDCGMIMVRNGNCYKCLNCGETSGCS"
     gene            complement(815..1585)
                     /locus_tag="AC16FU_01590"
     CDS             complement(815..1585)
                     /locus_tag="AC16FU_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR2993102.1"
                     /translation="MRFAVRRIAISILISLLGLAPVVLAGTAPGGAESAAEGKERGRA
                     GALALRGFFGGDAAADSSKKSDTLDPCGQPVPPNLFQKVSTTGERPAGSPNASPATVL
                     RKADLPKVPGGPKGTIYFFLGSLCPCTDAHRNSIAELMSLLPKRRVKPVAIFPNRGES
                     VELVEHFFRGLGFKMAYVIDSTNRLVDKFRAKKTPEVFLVDTKGKVVYSGSIDDSVEN
                     LGQVKHAYLKNAVMDLTDGRPVAVSRAEGSGCWIVRKK"
     gene            complement(1596..2666)
                     /locus_tag="AC16FU_01595"
     CDS             complement(1596..2666)
                     /locus_tag="AC16FU_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR2993103.1"
                     /translation="MKKSKAVWAVAAGLVVLGGGWLWLAGCGGSAKGGIDTKHMVYGA
                     PESKMSMTPASGPLFEGAALSIAPEVKPVPNTNEHHVRIDVTHSEIVVNDTVKFIGWV
                     FGGMLPGPVLHVRQGDKVYFTMTNRSNETVGTSPPSPHSIDFHAAMVNPKDKYREVIP
                     GATIEFEWTANYPGVYMYHCGSPAILQHMIYGMIGMTIVEPKDGYPTKVDREYALFQS
                     ELYLTKLKSGMYVTDMVTAKKKEPMFVCFNGKPAQYVKNPLKARAGERVRLYVLNAGP
                     NGTSSFHVVGTLFDRAWMEGNPLNEMRGAATILLPSSGGAIVEFVVPEKGIYTFVDHE
                     FADVEAGAVGLIDATSEEVAVK"
BASE COUNT          525 a          878 c          736 g          581 t
ORIGIN      
        1 tttaccgcga cggctccaag cggacgcagc cgctcgttac gggcttgggc gagccaactg
       61 caaccgtcgc tgcgccgcgc cgccgccgcc tacccgacga acgcaaggcc gtcacccaca
      121 agttctccat cgccgggcac gaggggtaca tcaccgtggg gctttacgat gacggcgtcc
      181 cgggcgaaat cttcatcacc atggccaaag agggctcgac cgtctccggg ttgatggatt
      241 ccttcgccac tgccatttcg ctcgccctcc agtacggcgt tccgctcaag accttggtgg
      301 agaagttctc ccacacccgc ttcgagccgg ccggcttcac caacaatccg gagattccgt
      361 tagcccgttc ggtgatggac tacatcttcc gctgggtggg gaagaagttc ctcccgccgg
      421 aagaccaacc caaaggacaa acgtcggcca ccaccgaaga aaacggcggc acgaagaccg
      481 caaccgctca tccggtaggg aaacagacga ctcttttcgc cggagagaaa accgacacct
      541 tcgaaaacca gaccgacgct cccgcctgcc ccgactgtgg aatgattatg gtccgcaacg
      601 gcaactgcta caagtgcctg aactgcggcg aaacctcggg gtgcagttaa tttttacggt
      661 tacgttttga tgtcggggct gggtctccca gcctcaatgc ccgtggcgcg ggttggtttt
      721 gtaacccgcg ctttgctttt tagcggagct gacgatttcc tccgtgccct ctcgaagagt
      781 cgtgccgact gtgtttccgt gttggaattt tcttttattt cttccttaca atccagcacc
      841 cgcttccttc ggctcgggac acggccaccg gtcgcccgtc ggtcaaatcc ataacagcgt
      901 ttttgagata ggcatgcttc acctggccta aattctccac cgaatcgtca atcgatccgg
      961 agtagaccac tttccccttg gtatccacca gaaagacctc tggtgttttc ttggccctga
     1021 acttgtccac taagcggttg gtcgaatcga tgacgtaggc cattttgaat cccagccctc
     1081 tgaagaaatg ctcgaccaac tcgaccgact ctccgcggtt ggggaaaatg gcgaccggct
     1141 tcactctccg cttgggcaaa agcgacatca gttcggcaat cgaattccgg tgcgcgtcgg
     1201 tgcagggaca gagcgacccc aaaaagaagt agatcgtccc ttttggcccg ccgggtactt
     1261 tggggaggtc ggccttgcgc aacaccgtcg ctggggaagc gttcggtgaa cctgcgggac
     1321 gctcccctgt cgtgctgacc ttctggaaca aattgggagg tacaggttgt ccgcacgggt
     1381 cgagcgtgtc gcttttcttg ctcgaatcgg cggctgcgtc ccctccaaaa aacccgcgta
     1441 gggcgagcgc ccccgctcgc cctcgttctt tcccttctgc cgccgattcc gccccgcctg
     1501 gggcggtccc ggccaatacg accggggcca gccccaagag ggaaatcagt atggaaatcg
     1561 caatccgccg cacggcaaat ctcaaaaaga gacgcctact ttactgccac ttcttcgctc
     1621 gtggcgtcaa tcaagcccac cgcgcctgct tccacgtcgg cgaactcgtg atccacaaag
     1681 gtgtaaatcc ccttttcggg aacgacgaat tccacaatcg ccccgcccga agaaggcaaa
     1741 agaatcgttg ccgccccgcg catttcgttc aagggattcc cctccatcca ggcccggtca
     1801 aagagcgtgc ccaccacgtg gaaactggag gtgccgttcg gcccggcgtt caaaacgtac
     1861 agccgcaccc gctccccggc tctggccttc aggggatttt tgacgtactg cgccggcttg
     1921 ccattgaagc agacgaacat cggttctttc tttttcgctg tgaccatgtc ggtcacgtac
     1981 atccccgatt tcagtttggt caggtagagc tccgactgaa agagcgcgta ctcgcggtca
     2041 actttggtgg gatagccgtc cttcggctcg acgatcgtca tcccgatcat cccgtaaatc
     2101 atgtgctgca ggatcgccgg cgacccgcag tggtacatgt agacgcccgg gtaattggcc
     2161 gtccactcaa actcgatggt ggcgccggga atcacctccc ggtatttgtc cttcggattg
     2221 accatcgccg cgtggaagtc gatcgaatgc ggcgaaggcg gcgaagtgcc caccgtctcg
     2281 ttggagcggt tggtcatggt gaagtagacc ttgtccccct gccgcacgtg caaaaccggc
     2341 ccggggagca tcccgccgaa aacccagccg atgaatttga ccgtgtcgtt caccacgatt
     2401 tcggagtggg tgacatcaat tctgacgtgg tgctcgttgg tgttcggcac cggcttcact
     2461 tccggcgcga tggagagcgc cgcgccttcg aagagcggtc ccgacgccgg cgtcatcgac
     2521 attttggact ccggcgcgcc gtagaccata tgcttggtgt caatcccgcc tttggcgctg
     2581 ccgccgcagc cggcgagcca gagccaaccc ccccccagta cgaccaaccc cgctgccact
     2641 gcccagactg ccttactctt tttcacttcc tcctcctttg ttgctcccgc ctgttatcga
     2701 atccgacgtg aaatgattat
//
LOCUS       DBNGZA010000078        11854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2241923, whole genome shotgun sequence.
ACCESSION   DBNGZA010000078 DBNGZA010000000
VERSION     DBNGZA010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2241923"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..222)
                     /locus_tag="AC16FU_01600"
     CDS             complement(31..222)
                     /locus_tag="AC16FU_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993104.1"
                     /translation="MKFLAPVVLLLGVVVYQSLFSELFSIAEIKPDLALALTVYLALA
                     RGPEAGLAFGFFSGLCLDV"
     gene            complement(219..1046)
                     /gene="mreC"
                     /locus_tag="AC16FU_01605"
     CDS             complement(219..1046)
                     /gene="mreC"
                     /locus_tag="AC16FU_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR2993105.1"
                     /translation="MRAFPVLIAKYRPTLLLITGLVISGGTAVLPVPVKFGVANFLVR
                     IVYAPFFRISNKIDELKSLYVANRDLNRVLAELSLENARLRTAAEENARLRELLGFAE
                     PLPYHLIPAEVITLDPQYPFRSALIAAGTERGVRRFLPVIDRFGVAGKISAVTARQAE
                     VQLLFDPGFRAAARDLRSGVLGIVRWKKEFLLAFDNVPNREDVQIGDSVITSGLGGVF
                     PEGLPLGRVIRVQTDSMAFFKSILVEPLARLATVDELFVLALPPATRSGALEGEPEP"
     gene            complement(1077..1538)
                     /locus_tag="AC16FU_01610"
     CDS             complement(1077..1538)
                     /locus_tag="AC16FU_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR2993106.1"
                     /translation="MKLSKFCEEDLITFELKSETKDDVIRELVELAARSKMVRDKVEL
                     SRAVVEREKLVTTGVGYGVAFPHAKTKAVKGVVIAFGRSKEGIDFEAMDRKPVHLFFL
                     IAAPEDAIGAHLNVMARLSYIMRSEKNREKFGTIKSPGEFLEQLDLASEAT"
     gene            complement(1582..1758)
                     /locus_tag="AC16FU_01615"
     CDS             complement(1582..1758)
                     /locus_tag="AC16FU_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR2993107.1"
                     /translation="MMLDEKFLSILACPKCKGELAYRPELQRLICPACRLQYPIQDDI
                     PILLVEKGEPLPQG"
     gene            complement(1771..2751)
                     /locus_tag="AC16FU_01620"
     CDS             complement(1771..2751)
                     /locus_tag="AC16FU_01620"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR2993108.1"
                     /translation="METQTYLDFEKPIAELEKKIKDIRDFATGEAVELDREIKGLQRR
                     LERLERDIYSNLSRWQRVQLSRHPRRPLALDYIVLITTEFIELHGDRGFADDRALVGG
                     FAKIDGRKVLMLGQQKGRDTKQKLYHNFGMMHPEGYRKALRLMLLAEKYRKPVVVLVD
                     TPGAFPGVGAEERGQAEAIARNLREMARLSVPIIVVIIGEGASGGALGVGVGDLILML
                     ENAWYSVISPEGCAAILWRDAEKKPLAAEALKLSAFDLLGLGIIDKIIPEPLGGAQRD
                     PESQAARVKTEILQGLEALETLAPEELVTRRLQKYREMGAYHELLPGENL"
     gene            complement(2753..3829)
                     /locus_tag="AC16FU_01625"
     CDS             complement(2753..3829)
                     /locus_tag="AC16FU_01625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993109.1"
                     /translation="MLRYALVFAVAGLTTLFLTPLVLRLCWRYGWFDHPNGRKVHQNP
                     VPRLGGVAIFFGFWLAVAVGLARSPDRFYLATEQFAPIFFGSLVILAIGLYDDLKGAV
                     PAAKLLAQITAGLILIAWGIQIRVLYIPFVGGVALGATSVPVTLLWLLALANTINLID
                     GLDGLAAGISAIVAASLLLTGLFLKIESVAIISAAVMGATLAFLRFNWHPARLFMGDS
                     GSLVLGFLFASVSIICPVKSITGVALFVPLLALGVPLIETASAIVRRISAKQKLLAAD
                     NQHLFHYLLGLGLSQRAVVWLFYVAACACLFVVLCLLTLDRRVIVSALMVLYVMLFLA
                     LAFALRQLTGRSEKGVLNAPPEKV"
     gene            complement(3823..4776)
                     /locus_tag="AC16FU_01630"
     CDS             complement(3823..4776)
                     /locus_tag="AC16FU_01630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993110.1"
                     /translation="MATDLSIIIVNYNTADCLVHCLSALFGSPPAMAWEVLVVDNASS
                     DRSVPCLRSDFPQVRLLVNNRNLGFGAAGNRGFAKAKGEFILFMNADVIVCPQAIDQL
                     VRTLRSRPEVGVCGGKLVSPDGTVQPTFRRFPTHRSILFARGSLLGKIFPDRRGRYVL
                     PEVDDTVAVDSVAAAFLLTRRSALEQMQGFDERFFLYAEDLDFCRRMKDAGYEVLYVP
                     RALATHQWGASTRQNRSLAWRAHHRSIYRYFRKHYPQKKVSNFFLYLLLQAQLFLLLA
                     LNFWRREEAAEGNPVSKAAALPAGRTQSEATAAVSATGLSC"
     gene            complement(4804..5517)
                     /locus_tag="AC16FU_01635"
     CDS             complement(4804..5517)
                     /locus_tag="AC16FU_01635"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR2993111.1"
                     /translation="MRALVAIPTYNEKENLEKIVPVVLAQDVCLEVLVVDDNSPDGTG
                     QIADRLAQANPRVHVLHREKKSGLGQAYLAAFRWALEHGYDLVFEMDADFSHDPKYLK
                     DFLVAIKEADLVIGSRYISGVNVINWPMGRLLLSYYANVYTRLVTGLPLRDATGGFKC
                     YRRKVLETIDLAAVRSNGYSFQIEVSFRAWKKGFRLREIPIIFIDRQAGTSKMSKKIV
                     REAVWIVWRLRLWSLLGRV"
     gene            complement(5617..6519)
                     /locus_tag="AC16FU_01640"
     CDS             complement(5617..6519)
                     /locus_tag="AC16FU_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR2993112.1"
                     /translation="MPEERRPHWWSAPVKAILLAVIIFFIAKTLLANWRQVRTFPWRF
                     NIPLLLSATVLQYSVLLYLIEVWRRVLAYFGGRLSFRAAYRIWVVANLGKYLPGKVWS
                     VVGLATLAQRAGVTAELTVAVAMLTSALTVLGAFVVVFLFSGTSHFAGIPPSVYFVFV
                     SICLVAVYPRWLFRLLNLALGFLKRDPIRLAVPPGLPFVLLIFYLVAWVLYGGSFFLF
                     LRALGLGTWHQFFPITAAFIFSYLIGFLALFVPGGLGVREGLLTLSLSAYLPAGVATA
                     VAIGSRLWMTAIELLALLGIRRAR"
     gene            complement(6613..7710)
                     /locus_tag="AC16FU_01645"
     CDS             complement(6613..7710)
                     /locus_tag="AC16FU_01645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993113.1"
                     /translation="MPNYPSRPRFRHQSHQPRFSVVVPAYNEAENIPILVEKFVETFR
                     QNRLFGEVVIVDDGSTDRTYLRAREYQEKFRYIRVISHKTNMGLTAALQTGFQAARAD
                     VLVFYPADLQYLPEDLPKLLNRLNEGYDIVTGWRQGHYGLKRFVSFVYNLLSRLLFRM
                     KVHDLNSIKAFRREVIEAIPFRRDWHRYFVVIAAERGFKISEVKVRLYPRKYGKSKFG
                     FWRIPVGMLDLIAVKFQISFMQKPLLFFGSIGLFLLGLAVLVGVVAVILRFGLDEGYR
                     PLLYLVMLLASIGMVNFALGFLAEGLQIIREKLEDLEKKLPATPAAVVQSPPQLPRPR
                     PQMRPQPQFPQPRPPAQEPQAPPGEPETMQS"
     gene            7838..8899
                     /locus_tag="AC16FU_01650"
     CDS             7838..8899
                     /locus_tag="AC16FU_01650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2993114.1"
                     /translation="MRATTETAINEIRAALSDLWDISVIYTTPAELVIQGQPRQSLEL
                     DGVTTRARLAALGYQVLIEPHPDQTLLILRRTEATAARRIPWVNIILFFATLLTTSLM
                     KPLTQPGVSLSDRPRLILEWLPYSGALLTILVCHEFGHYFMSKARGVQTSLPYFLPAP
                     TLFGTFGAFIKSKSYFRNRSDLLAIGAAGPIAGFIVALPVLFIGLSQAKIVPAGGGGM
                     NFGESLLTWAIASITHGPLPKGTALEAGPLLLAGWVGVLVTMMNLLPLGQLDGGHILY
                     GLLGKKQVTIARWAFLALLPLGLLWSGWWFWGALALVFRLRHPPTLDDTAPLDFPHKL
                     MAFACGLIFILCFIPVPFS"
     gene            complement(9310..9894)
                     /locus_tag="AC16FU_01655"
     CDS             complement(9310..9894)
                     /locus_tag="AC16FU_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993115.1"
                     /translation="MTVRWHNLGTVIVNENGEKEYILNEEGAGIPILLALLGQDGMVL
                     PEIPWCGGIFWRYAYFNNGKDTSFLCPVFQYDFPSLNAEPGATSIGWGTARNEEEAER
                     WNRDFADRCWDEFQTGCINYTYTLLEWLPKEKLRDPKAVCFVYPGRQLGRDFTFYVLQ
                     WKGGQAPFRSMAGVDGSGIILRAWDLNTKKKAQK"
     gene            complement(10113..11711)
                     /locus_tag="AC16FU_01660"
     CDS             complement(10113..11711)
                     /locus_tag="AC16FU_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR2993116.1"
                     /translation="MTKVQVSRILVVDDEQYICDIVREALVSHPEYQIQTTADPRRAV
                     EILTNEPVDLVLSDLIMGKITGVEILEVARKLHPDSIVILMTGQPTVENAVEVIKQGA
                     YDYLIKPFTLETLRATVERGLERQRLARENVHLKEQMGLFRISEAMGSTIHLDQLLTL
                     MLDAIIKEFDADMASILLVDKITGELKLNAFRGNTSDSAGSWFLLGHDEVCRWVVDNG
                     LPKMVNEKQERRRRTSPGLKQNVKSFVCYPLLARGKVLGVLNVVRTEKMAEFSQGQLQ
                     SLGIIVSKAASAIESSMLYEELQDAYLDTIIVLANAIEARDSYTRLHTDRVRYLAELI
                     ARELGWPDARMREVWMGGILHDVGKIAVPDGILNKRGPLSPEEFTIMRQHPQTGAKML
                     ESIPFLAPAIPFVLYHQERFDGTGYPFGLKGESIPIEGRLLSVVDTFDAVVSDRPYRR
                     GRSFEEAITELVRCAGTQFDPHVVELFVKLWNEEKIDRMLVATGKVDNPYRDTIQPET
                     SQTRKLRMSQIPVGANTGKVIVQP"
BASE COUNT         2640 a         3536 c         3291 g         2387 t
ORIGIN      
        1 gaccatcccg gtgccccgag agagcggact tcacacatcc aggcaaagtc ctgagaaaaa
       61 gccgaaggcg agccccgctt cgggaccccg ggccaacgcc aggtacaccg tgagtgccag
      121 cgccagatcg ggcttgatct cggcaatgct gaacagttcg gaaaaaagcg attggtacac
      181 gaccactcct aaaagcagca ccacaggtgc gaggaacttc acggttccgg ttctccttcc
      241 aaagcgcccg aacgggttgc cggcggcagt gccagaacga acagctcatc caccgtcgcc
      301 aggcgcgcca aaggttccac caaaattgac ttgaagaacg ccatgctatc ggtctgcacc
      361 cgaatgacac ggccgagcgg caacccctcc ggaaagacgc cccccagtcc ggaagtaatg
      421 acggaatcgc cgatctgcac gtcttcccgg ttgggcacgt tatcgaacgc cagcaggaac
      481 tccttcttcc accggacgat tcccaaaacg cccgaccgca ggtcgcgcgc cgccgcccgg
      541 aacccggggt caaagagtaa ttgcacctcg gcctgacggg cggtcaccgc gctgattttg
      601 ccggctacac cgaagcggtc gatcacgggc aaaaaccgcc gcaccccccg ttcggttccg
      661 gcggcgatga gcgccgaccg aaatgggtac tgcggatcca gcgtaatgac ttcggccggg
      721 atcagatggt aggggagcgg ctcggcaaac cccaaaagct cccgcagacg ggcgttctcc
      781 tctgcggcgg tccgcaaccg ggcgttctcc agcgagagtt cggccaaaac ccggttcagg
      841 tcacggttcg ccacgtagag ggacttcagc tcatcgattt tgtttgaaat gcgaaaaaaa
      901 ggagcataga cgatccggac gaggaagttg gccacgccaa acttgaccgg cacgggcaac
      961 accgccgtgc cgccggaaat gacgagaccg gtaatcaaaa ggagggtcgg ccggtatttg
     1021 gcaataagaa cggggaaggc gcgcatacgt cgaacgcggt ttccgcggtg ccccggctac
     1081 gtcgcctcac tggccaaatc gagctgttcc aaaaattcac ccgggctttt gatcgtgccg
     1141 aatttctccc ggttcttctc gctgcgcatg atgtaggaga gtcgcgccat gacgttcagg
     1201 tgggcgccaa tggcgtcttc cggcgcggcg atgagaaaga agaggtgcac cggcttgcgg
     1261 tccatcgcct caaaatcgat cccctccttg gagcgcccaa aggcgatgac cacccccttg
     1321 accgctttgg tcttcgcatg ggggaaggcg acgccgtagc cgaccccggt agtcaccagc
     1381 ttctcccgct ccaccaccgc ccgggagagc tccactttgt cccgcaccat ttttgaccgg
     1441 gccgccagct ccaccagttc gcggatgacg tcgtctttcg tttcgctctt cagttcgaag
     1501 gtgatcaagt cttcttcgca gaatttggaa agtttcatcc ctggcctccg tagcgactat
     1561 cggttgttcg gaaagaaaaa tctacccctg cgggagcggc tccccttttt cgacgagcag
     1621 aatgggaatg tcgtcctgga tcgggtattg caggcggcag gccggacaaa tcaagcgctg
     1681 tagctccggc cggtacgcaa gctccccttt gcatttcggg caagctaaga tggacagaaa
     1741 tttttcgtct aacatcattt gtccttaccc ttagaggttt tccccgggca gcagttcatg
     1801 gtaggccccc atctcccggt acttctggag tcgccgggtc accagttctt ccggggcgag
     1861 tgtttccagg gcttccagcc cttggagaat ttcggttttc acccgggcgg cctggctttc
     1921 ggggtcacgc tgcgctccgc cgagcggttc cggaatgatt ttatcgataa tccccaatcc
     1981 caggaggtcg aaagcggaca gcttgagcgc ctcggccgcc agaggttttt tctccgcatc
     2041 gcgccataaa atggcagcgc acccttccgg ggaaatcacc gaataccagg cattttccag
     2101 catcaagatg agatctccca cgcccacacc caaggcacca ccggaagcac cttccccgat
     2161 gatcaccacg atgatcggga ccgagagccg cgccatttcg cgtaggttgc gggcgatggc
     2221 ttcggcctgg ccccgttctt cggcgcccac gccggggaac gcccccggcg tatcgaccag
     2281 caccaccacc ggctttctat atttttcggc caagagcatc aaccggagcg ccttgcggta
     2341 cccttccggg tgcatcatcc cgaagttatg gtagagtttc tgcttggtgt cgcgcccttt
     2401 ctgctggcct aacatcagca ccttccgtcc gtcaattttg gcgaacccgc ccaccagcgc
     2461 ccggtcgtcg gcgaatcccc ggtcgccgtg cagttcaatg aactcggtcg tgatgagtac
     2521 gatgtagtcc agcgccagag gtcggcgggg gtggcgggac aactgcaccc gctgccagcg
     2581 cgagaggttg gagtagatat cccgctccag ccgttccagg cgtctctgga gtcctttgat
     2641 ctcccggtcg agctccaccg cctcgccggt ggcgaagtcg cggatgtcct tgattttttt
     2701 ttctaactcg gcgatcggtt tttcgaaatc gaggtaggtc tgggtttcca tattagactt
     2761 tctccggtgg ggcattgagg acgcctttct ccgagcgtcc cgtcagttgc cgcagcgcaa
     2821 aagccaaagc cagaaaaagc ataacgtaga gaaccatgag tgccgaaacg atgacccgcc
     2881 ggtctaaggt caaaaggcaa aggacgacaa agagacacgc gcaagcggcc acgtaaaaga
     2941 gccagaccac cgcccgctgg ctcaagccca atcccagaag gtagtgaaag aggtgctggt
     3001 tgtcagccgc caaaagtttc tgcttggccg agatccggcg gacgatagcc gaagcggttt
     3061 cgatcaaggg cacgcctaat gccaaaagcg gtacgaaaag cgccaccccg gtgatacttt
     3121 tcaccggaca gatgatcgaa accgaagcga agaggaaacc caggactaga gagccggagt
     3181 cccccatgaa gagcctggcc ggatgccagt tgaagcgcag gaacgccagg gtggctccca
     3241 tgacagccgc ggagatgatg gccaccgact cgatttttaa aaagagaccg gtaagaagca
     3301 aactcgccgc cacaatggcc gaaatgccgg ccgccaagcc gtccagtcca tcaatcaaat
     3361 tgatggtatt ggccaacgcc aaaagccaga gcagagtcac cgggacgctg gttgctccca
     3421 acgcgacacc ccccacaaac ggaatgtaga ggacacggat ttgaataccc caagcaatca
     3481 agatcaaacc ggcggtgatc tgggccaaca gtttggctgc gggaactgct cccttgaggt
     3541 cgtcgtaaag tccaatggcc agaatcacca gagaaccgaa aaatatgggc gcaaactgtt
     3601 cggtggctag gtagaaccgg tcgggagaac gcgcgagtcc caccgccacg gccaaccaga
     3661 aaccaaagaa gatagccacg ccgcccaagc gcggaaccgg attttggtgc actttgcgtc
     3721 cgttgggatg atcaaaccag ccatagcgcc agcagagccg caagaccagc ggtgtcaaaa
     3781 acaaggtcgt cagccccgct acggcaaaga ccagagcata cctcaacatg acaaacccgt
     3841 tgcggagacc gccgccgtgg cctcagactg ggtacgaccg gcggggaggg ctgcggcttt
     3901 gcttaccgga ttcccttccg ctgcttcctc cctgcgccag aaattgaggg cgagcaacaa
     3961 gaataattgt gcctgaagca ggaggtacag aaaaaagttg gacactttct tctgtggata
     4021 atgctttcgg aaatagcggt aaatgctccg gtgatgtgcg cgccaagcca gcgagcgatt
     4081 ttgccgggtg ctggcacccc actggtgagt ggccaaagca cgaggaacgt agagcacttc
     4141 atagccagcg tccttcatcc ttctacagaa atcgagatct tcggcgtaga ggaaaaacct
     4201 ttcgtcaaaa ccttgcatct gctcgagtgc ggaacgtcgg gtgagcagga aggcggcggc
     4261 aacagaatcg acggcgaccg tgtcatcgac ttccggcaaa acgtagcggc ccctccggtc
     4321 gggaaagatt tttcccaaaa gagagccgcg ggcaaaaagg atgctccggt gggtcgggaa
     4381 gcgccggaag gtcggctgta ccgttccatc gggcgaaacg agcttgccgc cgcacacgcc
     4441 aacttcagga cgcgaccgga gcgttcgcac caactggtcg atggcctgcg gacaaacgat
     4501 gacgtcggca ttcataaata aaatgaactc gcctttcgcc tttgcaaaac cccggttacc
     4561 ggccgcgcca aacccgagat tgcggttgtt taccagcaac cgtacctggg ggaaatcgct
     4621 ccggaggcag gggacactcc ggtcggaaga ggcattatcc accaccagca cttcccacgc
     4681 catggctggc ggcgacccga agagggcgga gagacaatgg acgagacagt ccgccgtgtt
     4741 ataattgacg atgataatgg aaagatcggt cgccattgcc ataccgcgtc ggtagccggg
     4801 gcactacacc cgacccaaaa gactccacag ccgcaagcgc cagactatcc aaaccgcttc
     4861 gcgcacgatt ttcttggaca ttttggacgt cccggcttgt cggtcgataa agataatcgg
     4921 aatttctctc aaacgaaacc cttttttcca ggcccggaaa ctcacttcga tctggaacga
     4981 gtagccgttg gagcgtaccg cagccaaatc gattgtctcc agcaccttgc gccggtagca
     5041 cttgaagccg ccggtcgcat cccgcagcgg cagcccggta accagccgcg tgtagacatt
     5101 ggcatagtat gacaaaagca accgccccat gggccagttg atgacgttca cccccgaaat
     5161 gtaacgcgaa cctatcacta aatcagcttc tttgattgca accaaaaagt ctttcaggta
     5221 tttggggtca tgggagaagt cggcgtccat ttcaaacacg aggtcgtagc cgtgctcgag
     5281 cgcccagcgg aaggccgcca ggtaggcctg ccccaagccg gacttctttt cccggtgcag
     5341 gacgtgcacc cgtgggttag cctgtgccag ccggtcagca atctggccgg tgccgtccgg
     5401 cgagttgtcg tccaccacca gaacctctag acaaacatcc tgcgctaaga ccaccggcac
     5461 gattttttct agattctctt tctcattata cgtgggtatg gctaccagag cccgcatgct
     5521 cgctccgggc ctagtgggga ggggaatgaa agtccacgtg caattttcct ccagcacatc
     5581 acgtttgcat gaatatagac ggccgcttcg agagggtcaa cgcgcccttc gaattcccaa
     5641 aagcgccagg agctcaatgg ctgtcatcca gagccgcgag ccgatggcta cggctgtggc
     5701 caccccggca gggaggtagg cggacaaaga cagggtgagc aacccctccc gcactcccaa
     5761 accgcctggt acaaaaagtg ccaggaaacc gatgaggtag gagaaaatga aagcggcggt
     5821 gatgggaaaa aactggtgcc aggtacccag acccagcgcc cggaggaaga gaaaaaaaga
     5881 accgccgtag aggacccagg ctacgaggta aaaaatcagg agcacaaacg ggagtcccgg
     5941 cggcacggcc agccgaatcg ggtcacgttt gaggaaaccg agcgcgaggt tgagcaaccg
     6001 aaagagccag cgcgggtaga ccgccacgag gcagattgag acgaaaacga aataaaccga
     6061 cggtggtatg cccgcgaagt gactcgtacc ggagaaaagg aacaccacca caaacgctcc
     6121 caaaacggtc aaagccgagg tcagcatcgc caccgccacg gtcaattccg ccgtcactcc
     6181 cgccctttgc gccaacgtgg ccagacccac caccgaccag actttccccg gcaggtactt
     6241 ccccagattg gcaaccaccc agatccggta cgccgcccga aaggaaagac ggccgccgaa
     6301 ataggcgaga acccggcgcc aaacttcgat aaggtagagg aggacggagt actggagaac
     6361 ggtagcgctt aaaagaagcg gaatattgaa ccgccaggga aaagtccgca cctgccgcca
     6421 gttggccaaa agggtcttgg cgataaaaaa aataattacc gccaaaagga tggctttgac
     6481 gggcgcagac caccagtggg gtcgcctctc ctcaggcatg ggcggctacc cctatcccgg
     6541 tgaagatcgt cggcagccgc cggcaaactc cctccggccc gcaattggcc gcgccgaaca
     6601 gtcggacatg cgctatgact gcatcgtctc cggctcaccc ggcggcgcct gtggctcctg
     6661 cgccggaggc cgcggctggg gaaattgggg ctggggtctc atctgcggcc gtggccgggg
     6721 cagctgcggg ggagattgaa ccaccgcggc tggcgtagcg ggcagtttct tctccagatc
     6781 ttctaatttt tcgcggatga tttggagtcc ttcggccaga aaaccgagcg caaaattgac
     6841 cataccgatg gaggccagaa gcatcactaa gtaaaggagc ggccggtacc cttcatcgag
     6901 accgaaacgc aagataaccg ccaccacgcc caccagcacc gccagtccca aaaggaacag
     6961 cccgatagag ccgaagaaca gaagcggctt ctgcatgaac gaaatctgga acttcacggc
     7021 aatcagatcg agcatcccca ccggaatccg ccagaagccg aatttcgact tcccgtactt
     7081 gcgggggtag agccgcactt taacttcgct gattttgaaa cctctctctg ccgctatgac
     7141 cacgaaatac cggtgccaat cccgccggaa gggaatcgct tcaatcacct cccgccggaa
     7201 ggccttgatc gaattcaaat cgtgaacttt catgcgaaag agtagccggg acaaaagatt
     7261 gtagacgaag gagacgaagc gtttgagccc gtagtgcccc tgccgccagc cggtgacgat
     7321 gtcgtaccct tcattcaagc ggttgaggag tttggggaga tcctcgggca ggtactgcag
     7381 gtcagccggg taaaagacca aaacgtcggc ccgggctgcc tgaaagccgg tttgcaacgc
     7441 tgcggtcaag cccatattgg ttttgtggga gatcacccga atgtagcgga atttttcctg
     7501 gtactcccgg gccctgaggt aggtgcggtc ggtggagccg tcgtccacga tcaccacttc
     7561 gccgaagagc cggttctggc ggaaggtctc gacgaacttc tcaaccaaga tcgggatgtt
     7621 ttccgcctcg ttgtacgccg gcaccactac tgagaaacgg ggctggtgac tctggtgacg
     7681 aaagcgtggt cgggagggat agttaggcat gattctgtat ctcagccacc gggctggact
     7741 tctctatata aagcacgagg taagccgccg tcaagatacc cgaccgaaag aaaatcggat
     7801 actgcgcaac ccgtagctgt attatcttac tcacacgatg cgcgctacca cggaaacagc
     7861 cataaacgag atccgggcgg ctctgtccga cctctgggac atttcggtca tctacacgac
     7921 gcccgctgaa ctcgtgatcc aagggcaacc ccgacagagt ctggaattgg atggagtcac
     7981 gacgcgggct cgcctcgctg ccctgggcta tcaggtcttg attgagccgc acccggatca
     8041 aaccttgctc attctccgcc ggaccgaagc cacggcggcg cgcagaattc cgtgggtcaa
     8101 catcatcctc ttttttgcga ccttgttgac gacttcgctc atgaaacctt tgacgcagcc
     8161 cggggtctcc ctatctgacc gaccgcgcct gattctcgaa tggctccctt actctggagc
     8221 actcttgacc attttggtct gccacgaatt cgggcactac tttatgtcaa aggcgcgggg
     8281 tgtacagacc tccctgccct attttcttcc ggcgccgaca ttattcggaa cattcggtgc
     8341 tttcatcaag tcgaaatcgt atttccgcaa ccggagcgac ctcttagcca tcggcgccgc
     8401 cgggccaatt gcggggttca tcgtcgcttt accggtgctc tttattggtt tgagtcaggc
     8461 gaagattgtt cctgccggcg gcggaggcat gaatttcggc gagtcccttt tgacctgggc
     8521 gatcgcctct atcacccacg gcccgctccc caagggcacc gctctcgaag ccggcccgct
     8581 tcttttggcc ggctgggtgg gagttctcgt gacgatgatg aacctgctcc ccttagggca
     8641 gttggacggc ggccacatcc tctacgggct tttgggaaaa aaacaggtga ccattgctcg
     8701 ctgggctttc cttgcgcttt tgcctctggg tttgctctgg agcggctggt ggttttgggg
     8761 cgctctggct ttagtcttcc gcctgcgtca cccaccgact cttgacgaca ccgctccgct
     8821 ggacttcccc cacaaactga tggccttcgc ctgcggcctc attttcatcc tctgcttcat
     8881 cccggtgccg tttagttgag gggggatagt tacccgcgta ggcggagcac cgggtttgaa
     8941 aaccccacgc gactcagaat aaattaaatt ctaagtcagc atttggtaaa tcgaactgca
     9001 gtttactatt tcagacatac cggtctctac agggaaagta ctacggttgc gttcggtcat
     9061 tttccaaaca ctcagatgcc ccggagtcat tatatcaagc ccccggggct gtttttcttt
     9121 aaacctttac catattatta agtctacgaa cgaattgcaa aagttccttt gctgtcagta
     9181 gcggtaggtg gatggatttt ccagtagtca aagttatatc caactggttc atcatcagac
     9241 cacttttagt tcgtacggcc tgcccacgtc tcaccttgac tttcttcatt cccgctcctt
     9301 tctccctgtt catttctgtg ctttcttttt tgtgtttaag tcccacgccc gcaggataat
     9361 gccagacccg tccacaccgg ccatcgagcg aaaaggcgcc tgccctccct tccactgcag
     9421 aacgtaaaaa gtaaaatccc gtcctaattg gcgaccggga tatacaaaac acaccgcttt
     9481 cggatcccgt aatttctctt ttggtagcca ctccagcagt gtgtatgtgt agttaataca
     9541 accagtctgg aattcgtccc aacagcggtc ggcaaagtca cgattccacc tttctgcctc
     9601 ctcctcattt cgggccgtcc cccacccgat gctcgtggct cccggctccg cattcagaga
     9661 gggaaagtcg tactgaaaaa ctggacaaag gaaagaagtg tccttaccgt tgttgaaata
     9721 ggcataacgc caaaaaatcc ctccgcacca aggtatctcc ggcaagacca tgccgtcttg
     9781 ccctaagagg gccaagagta tggggatgcc cgccccctct tcatttagta tgtattcttt
     9841 ctctccattt tcattcacga tcaccgtgcc gagattgtgc cagcgtaccg tcacgctatc
     9901 tctttgtgcg ttcccggccg ccggaaggaa aagtgtcgtc aatataaata tgactacgag
     9961 tgcccttttc atatcccgct tctttctcta tatttttttc gttgtgtcag gtcttcagac
    10021 ctgacacttg cgtcgggttt gaaagcccga cgcaactcaa aaaacctctt gttgcacagc
    10081 agagtagccc cgctcatcac ttatggaaag ccctacggtt gcacaataac cttccccgta
    10141 ttcgccccca cgggaatttg cgacatccgc agtttgcggg tctggctggt ctccggctga
    10201 atcgtgtcgc ggtaggggtt gtccaccttg ccggtcgcca ccagcatccg gtcgattttc
    10261 tcttcattcc agagtttcac gaacagttcg acgacgtgcg gatcgaactg cgtgccggca
    10321 cagcgcacta attcggttat ggcttcttca aaagagcgtc cccggcggta gggccggtca
    10381 ctcactaccg cgtcgaaggt gtcaacgacc gagagcaaac gcccctcaat tgggatgctc
    10441 tcgcctttca ggccgaaggg gtagccggta ccgtcgaaac gctcctggtg gtagaggacg
    10501 aaaggaattg ccggcgcgag aaaaggaatc gattccagca tcttggcccc ggtctgcggg
    10561 tgctgccgca taatggtaaa ctcttctggc gagagcggcc cgcgcttgtt caaaatcccg
    10621 tccggcacgg cgattttccc cacgtcgtgc agaatgcccc ccatccagac ctcgcgcatc
    10681 cgcgcatccg gccagcccaa ctcccgggcg atcaattcgg ccaggtagcg cacccggtct
    10741 gtgtgcagcc gggtgtagga atcccgcgct tcgatggcgt tggctaaaac gatgatggtg
    10801 tcgaggtagg cgtcctgcag ttcttcgtaa agcatggaac tttcaatcgc cgaagccgcc
    10861 ttgctgacga tgatgcctaa agactgcaac tgcccctggg aaaattcggc cattttttcg
    10921 gtgcgcacca cgttgaggac gcccaacact ttgcctctgg ccaagagcgg gtagcagacg
    10981 aacgatttga cgttctgttt gagccccggc gaggtgcgcc ggcggcgttc ctgcttttcg
    11041 ttcaccatct tgggcaatcc attgtccacc acccagcggc agacttcgtc gtggcccaaa
    11101 aggaaccagg agccggccga atcgctggtg ttcccccgga aggcgttcag tttcagttcg
    11161 ccggtgattt tgtccaccaa aaggatcgag gccatgtcgg cgtcgaactc tttgatgatg
    11221 gcatcgagca tgagggtcaa gagctggtcc aagtggatgg tggaacccat cgcctcgctg
    11281 atgcggaaga gccccatctg ctctttcaag tggacgttct cgcgggcgag ccgctggcgc
    11341 tccagcccgc gttcgacggt ggcccgcagc gtctcgagag tgaagggttt gatcaggtag
    11401 tcgtacgccc cttgcttgat gacctcgacg gcattctcaa cggtcggttg cccggtcatc
    11461 agaataacga tcgagtccgg gtgcaatttt cgggcgactt cgaggatttc gacgccggtg
    11521 atcttgccca tgatcagatc ggagaggacc aaatcgaccg gctcattggt gagaatttcc
    11581 accgcccggc gaggatcggc ggtggtctgg atttgatact ccggatggga tacgagcgcc
    11641 tcccggacga tgtcgcagat gtactgttcg tcgtccacca ccaggatgcg gcttacctgt
    11701 actttggtca cacgggctcc ggtcgttggg tcagcggtat cgtagatttt atcggaatgt
    11761 tggagagaaa ggttcagccg gagcgcacgg cgaaaggcgc tcagtgccgc gtctacctgg
    11821 tagtcgggct cgtccccgac ccctcccgct cagg
//
LOCUS       DBNGZA010000079         2713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_757071, whole genome shotgun sequence.
ACCESSION   DBNGZA010000079 DBNGZA010000000
VERSION     DBNGZA010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_757071"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="purD"
                     /locus_tag="AC16FU_01665"
     CDS             <1..1011
                     /gene="purD"
                     /locus_tag="AC16FU_01665"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR2993117.1"
                     /translation="WKIAQSPRVKKIYAAPGNAGIAQETLGPHERVTCVPIQPTDFTG
                     LVEFARANQIDLTLVGPEQPLCLGIADEFLLHGLKIVGPTRKAALIEGSKAFAQDFMQ
                     RRNIPTASFCVFTQFESARTFVQQADYPLVIKASGLAAGKGAFVVESVEEAAAVLEQL
                     LVQRTLGEAGATVVVEKFLEGVEATVMAFTDGKTVVPLPPVHDYKRAYEGDRGPNTGG
                     MGAVAPTDRLDQKTLNTIVAEILEPAVNGLAAEGRPYQGILYAGVMLTESEPKVLEFN
                     CRFGDPETQAVLPLMKSDLVEILLALADRELELVPIDWSGQYACSVVLAAGGYPEKYE
                     TG"
     gene            1015..1287
                     /locus_tag="AC16FU_01670"
     CDS             1015..1287
                     /locus_tag="AC16FU_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HIR2993118.1"
                     /translation="MFGLRPREQEGTLIFSAGIKSDRGRLVTDGGRVVSVTAIHDRPD
                     QAVARAYVAAERIKFEGKQFRRDIGFSASRIERAAPRDRGKFAAVR"
     gene            1300..1794
                     /gene="purE"
                     /locus_tag="AC16FU_01675"
     CDS             1300..1794
                     /gene="purE"
                     /locus_tag="AC16FU_01675"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR2993119.1"
                     /translation="MATSKLKVPILILLGSTSDRPAMAGCEAVLGELGIGYRLEISSA
                     HRHPDRTRRLARTAERQGYQVIITAAGLAAHISGVVASESLLPVIGVPLAGSPLAGLD
                     ALYSMVQMPRGVPVATMALASSGPVNAAIFAAQILAIEDRQVKTRLTRWKAKLARRSS
                     PKRS"
     gene            1811..2608
                     /locus_tag="AC16FU_01680"
     CDS             1811..2608
                     /locus_tag="AC16FU_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993120.1"
                     /translation="MRTQHFKPETGWTFTFSVVAAAVLIFFPRPSGATISEDAKKHFN
                     AGVEAKKTGNSDKAIAEYEQAIAAAPEYVEARVNVANLYETKGLVPKAIEQFKALTQL
                     EPGKAEYRSKLAALYVRNRQFADAGALYEEITAKNPKDGTAWLDLAEVYKKTGQLAKA
                     VEAYKQAIAANPQNVEAHYNLGNAYLGKGLYGEAQKEYRQVLQVKPAHFKAAFNLAVA
                     VQSQDPESLEQFLPLYEEFVQKFKGNAQARPMLQKAQEIIKQGKGVK"
BASE COUNT          596 a          739 c          891 g          487 t
ORIGIN      
        1 tggaaaatcg cccagagccc ccgggtcaaa aaaatctatg cggcgccggg gaacgccggg
       61 atcgcccagg aaacgctcgg gccgcacgag cgggtgacct gcgtgccgat tcagccgacc
      121 gatttcacgg gcttagtcga gtttgcccgc gccaatcaga ttgacctcac cctcgtcggg
      181 ccggagcagc cgctctgtct ggggattgcc gatgagtttc tcctccacgg actgaagatc
      241 gtcgggccga caagaaaagc ggcgctgatc gaggggagta aagcctttgc ccaagatttc
      301 atgcagcgcc gaaacattcc gaccgccagt ttctgcgtat tcactcagtt tgaatccgcc
      361 cgaactttcg tacaacaggc agactacccc ctggtgatca aagccagcgg tctggcggcc
      421 ggcaagggtg cttttgtggt ggaaagtgtt gaggaagccg ccgccgtact cgagcaactg
      481 ctcgttcaga gaaccctggg ggaagcggga gcgaccgtcg tagtggagaa gttcctggag
      541 ggcgtggaag ccacggtcat ggcctttacc gatgggaaga cagtcgtgcc tctgcccccg
      601 gtccacgact acaagcgggc ctacgagggc gaccgcggac ccaatacagg ggggatggga
      661 gcggtggcgc cgaccgaccg gctggatcag aagacgctca acacgatcgt cgcggagatt
      721 ctggagccgg cggtgaatgg tctggccgcc gaaggacggc cctatcaggg aatcctctac
      781 gccggggtga tgctgaccga aagcgagccg aaagtgttag aattcaactg ccgctttggg
      841 gatccggaaa cgcaggcggt gctgccactt atgaaaagtg acctggtgga gattttgctg
      901 gctttggccg atcgggaact ggaactggtg ccgatcgact ggtcgggtca gtatgcctgt
      961 tccgttgtgc tggccgcggg ggggtatccg gaaaaatacg agacgggata agtaattttc
     1021 gggctccggc ccagagaaca ggaaggaacg ctcatcttct ctgccggaat caaaagtgac
     1081 aggggccggt tggtcactga cggcggccgg gtggtgagtg tcaccgcgat tcacgaccgc
     1141 cccgatcagg cggtggcccg ggcctatgtg gcggctgagc ggatcaaatt tgaaggaaag
     1201 cagttccgta gagatattgg tttcagcgcc agccggatcg agcgggcggc ccccagagac
     1261 agaggcaagt tcgcagccgt ccggtaaaga atgcgggtta tggccaccag taagctgaaa
     1321 gttccgattt tgatcctcct gggcagtacg tcggatcgcc cggcgatggc aggctgtgaa
     1381 gcagtgctcg gggaactggg gatcggctac cggttggaga tttcttcggc ccaccggcat
     1441 cccgaccgga ctcggcggct ggcgcggacg gccgagcggc aggggtacca ggtgataatc
     1501 accgccgccg ggctggcggc ccatatctcc ggcgtggtgg cgtcggaatc actcctcccg
     1561 gtgatcggcg tgccgcttgc gggctcgcct ttggccggac tggatgcgct ctactcgatg
     1621 gtgcagatgc cccgaggggt gccggtggcg acgatggcct tggcgtccag cgggccggtc
     1681 aatgccgcca tttttgcagc gcagattctg gcaatagagg atcggcaggt aaaaacccgg
     1741 cttacgcgct ggaaggcgaa gttggcacgg aggagcagtc cgaaaaggtc ttaaagaaaa
     1801 aggagcggca atgcgtacgc aacatttcaa accggaaaca gggtggacct tcaccttctc
     1861 ggttgtggca gcggcggttc tgattttttt tccaagaccc tctggtgcca cgatttcgga
     1921 ggatgccaaa aagcacttca atgccggtgt ggaggccaag aagacgggga atagcgacaa
     1981 ggccattgcc gagtacgaac aggccattgc cgcggcaccg gagtacgtgg aggcgcgggt
     2041 caacgtcgcc aacctgtacg agaccaaggg tctcgttccc aaagccattg agcaattcaa
     2101 agccctgacg caactggagc cgggcaaagc ggagtaccgg agcaaactgg cggcgctcta
     2161 cgtcaggaac aggcagttcg ccgacgccgg tgcgctctat gaggaaatca ccgccaaaaa
     2221 ccccaaggac gggaccgcct ggctcgattt agccgaggtc tacaaaaaaa cggggcagct
     2281 ggccaaggca gtggaggctt acaagcaagc gattgccgcc aatccgcaga acgtagaggc
     2341 gcattataac ttaggcaatg cctatttggg caagggcctg tacggcgaag cgcagaagga
     2401 ataccggcaa gtgttgcagg tgaagccggc gcactttaag gccgccttca acttggccgt
     2461 ggcggtgcag agccaggatc ccgaaagcct cgagcagttc ctgccgctct acgaagagtt
     2521 cgtgcagaaa ttcaagggca acgcgcaggc gaggccaatg ttgcagaaag cccaagaaat
     2581 tatcaaacag ggcaaaggcg taaagtagat ggcggggata tttttccttc tggtcatcct
     2641 cttcgccacg ccggtggtac tgttcatcgt ctactacaac cggctcgtcc gcttgcgaac
     2701 gggaagcgag gcc
//
LOCUS       DBNGZA010000080         1161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_333173, whole genome shotgun sequence.
ACCESSION   DBNGZA010000080 DBNGZA010000000
VERSION     DBNGZA010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_333173"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16FU_01685"
     CDS             <1..258
                     /locus_tag="AC16FU_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HIR2993121.1"
                     /translation="LNSTKSMLGHLLGAAGAVELVVAIKSLATGIIHPTINLDDPDPQ
                     CDLDYTPHQPRQKTVRVALSNSFGFGGHNVTLAVRSFNGQP"
     gene            309..1103
                     /gene="rnc"
                     /locus_tag="AC16FU_01690"
     CDS             309..1103
                     /gene="rnc"
                     /locus_tag="AC16FU_01690"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR2993122.1"
                     /translation="MRLRFFELFRSFGRRQNPEEAPLAAVERLFGYAFRERSLLATAL
                     THRSSTKSQMEQRNGGAASRETSYERLEFLGDAVLGLLISEYLYRQYPDQDEGELTKL
                     KAALVNEVSLSRVARTIGLGDHLFFSPEGEKSGARQRNSILADAYEAVIGAIYLDGGL
                     APVRRAMARQMLSLLDDLLYDTSHFNFKGELLEFLQARALGMPRYQVVSERGPDHAKE
                     FTIAVSVQGETLGTGSGSTKKEAEQKAAAAALEKLSGQKVNSLEAL"
BASE COUNT          235 a          347 c          341 g          238 t
ORIGIN      
        1 ttgaactcca ccaagtcgat gctcgggcac ctcctgggtg ccgccggggc ggtggaactc
       61 gtcgtcgcca ttaaatcgct ggcaacgggg attatccatc ccacgatcaa cttggacgac
      121 ccggatcccc agtgtgatct tgactatacg ccgcaccagc cgcgccaaaa gacggtacgg
      181 gtggcgctct ccaattcttt cggttttggc gggcacaacg tcaccctggc cgtccgcagc
      241 ttcaacggcc aaccgtagtt tttttcactg gtaaaatcat cggactttgg ctacatttac
      301 ggaaacgtat gcggctgcgg tttttcgagc tgttccgaag ttttgggaga aggcagaacc
      361 cggaagaagc gcctttggct gccgtggagc ggctcttcgg ttacgccttc cgtgagcgtt
      421 cccttttggc cacggcactc acccaccgtt cttccaccaa aagccagatg gagcagcgca
      481 atgggggggc ggcttcccgg gagacttcct acgaacggct ggaatttctg ggcgacgccg
      541 ttttaggttt gctgatttcc gagtaccttt accgtcagta tccggaccaa gacgaagggg
      601 aactcaccaa actcaaagcc gccttagtca acgaggtgag cctgtcgcgg gtggcacgga
      661 cgatcgggtt gggcgaccat ctgttcttct cgccggaggg ggagaagagc ggcgcccggc
      721 agcgcaattc cattctggcc gacgcctacg aagcggtcat cggcgccatc tatttggacg
      781 gcggcttagc gccggttcgc cgggcgatgg cgcgtcagat gctctcctta ctggacgacc
      841 tcctctacga caccagccat ttcaatttca agggcgaact tttggagttc ctccaggcgc
      901 gggctttggg aatgccgcgc taccaggtgg tctccgagcg cggccccgat cacgccaagg
      961 aattcaccat tgccgtttca gtccagggag aaactctggg aaccggcagc gggtcgacca
     1021 aaaaggaagc ggaacaaaaa gccgccgccg ccgcgctgga gaaattgagc ggccagaagg
     1081 tcaattcact ggaggcgctc taaccggagg cacctacccc atgcacgtca tcgtctgcat
     1141 caagcaggtt ccggatatcg c
//
LOCUS       DBNGZA010000081         3245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_226867, whole genome shotgun sequence.
ACCESSION   DBNGZA010000081 DBNGZA010000000
VERSION     DBNGZA010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_226867"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..732)
                     /locus_tag="AC16FU_01695"
     CDS             complement(19..732)
                     /locus_tag="AC16FU_01695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR2993123.1"
                     /translation="MAQERDQANLFGLAQAALKQQTEFGFSEIYFDEALPPAQRFSPT
                     GEAIPGLEAQTLYELYDKIKNCQRCPLGASRTNLVFGSGNPQAAIVFVGEAPGRDEDL
                     QGEPFVGRAGQLLTKIIEAMKMKREDVYIANVLKSRPPENREPQPEEIAACFPYLQKQ
                     IELIKPKFIVCLGRIAAVTLLNTKLPLGKLRGALHEYRGIKVLVTYHPAALLRFPSYK
                     KDVWEDMKILRKAYDGTVL"
     gene            complement(693..1946)
                     /gene="coaBC"
                     /locus_tag="AC16FU_01700"
     CDS             complement(693..1946)
                     /gene="coaBC"
                     /locus_tag="AC16FU_01700"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003348955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR2993124.1"
                     /translation="MLTGKKILVGVTGGIAAYKAAYLVRRLRQAGVEVRVVMTKSATQ
                     FITPLTMETLSERPVGIEMFPEREFVGTHHITLADWPELVVVAPCTANVIGKVAGGLA
                     DDLLTNVLIATRAPVLLVPAMHDGMWANPQVQKNIVRLKEEGVHVLDPDIGPLASGDF
                     GPGRLPEVEVILARIEELVGGQGPLKGKKVLVTAGRTEEPLDPVRYLSNRSSGRMGIA
                     LAEAARDTGANVTLVHGPVSIALPAGVTSVSVSSAQEMYAMVADRFDACDVLLMAAAV
                     ADYAPEKPVAHKLKKTKKTLTVTLAPTVDILAEMGREKDKQILVGFALETEQEVKQAR
                     EKLQAKNVDLMVVNNPLEPGAGFEVETNRVTLLFRDGETVPLPLLRKRQVADRIVAEV
                     VNLLKARERKSTQWPKRETRPTYSD"
     gene            complement(2049..>3245)
                     /gene="purH"
                     /locus_tag="AC16FU_01705"
     CDS             complement(2049..>3245)
                     /gene="purH"
                     /locus_tag="AC16FU_01705"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR2993125.1"
                     /translation="IEQIDIGGPTLVRAAAKNHRFVTVVTSPADYPALKDELQKNQGS
                     VSLSLRQQFAQRAFAHTAAYDGAITEYLSRQSKTGEIFPEQLSGSFARVQALRYGENP
                     HQQAALYRKSDYRGPSLATAKLLSGKEPSYNNLMDLDFALSTVLDFSEPCAVIVKHAN
                     PCGLACDNSLAQAYQDAYDCDPLSAYGCIIGLNRKVDVETAQRIDATPFVECVLAPDY
                     APEVLELLQKKKNRRLVACLEMLQKNALPRRVARVISGGLLVQTPDTYRVGTPDLKTV
                     TQKAPTADQIASLLFAFHAVKQVKSNAIVIAQKTKTVGIGGGVTSRVDAVRLALDKAG
                     DRAKGAVLASDAFFPMPDSIDLAARAGIAAIIQPGGSKADPEVIAACDQSSIAMVFTG
                     IRHFRH"
BASE COUNT          669 a          932 c          926 g          718 t
ORIGIN      
        1 taactacccg ttcctgtttt acaacacagt gccatcatac gctttccgca atattttcat
       61 atcttcccag acatcttttt tatacgaagg gaaacgcaaa agcgccgccg ggtgataggt
      121 gacgagcacc ttgatgcccc ggtactcgtg gagagcaccg cgcagtttgc ccagcggcag
      181 tttggtgttc aaaagcgtca ccgcggcaat ccggcccaag cagacgatga acttcggctt
      241 gatcagttca atttgcttct gcagatacgg aaaacaggcg gcgatttctt cgggctgtgg
      301 ttcgcggttc tccggcggcc ggcttttgag cacgttggct atatagacat cctcccgttt
      361 cattttcatc gcttcgatga ttttggtgag cagctgcccg gcccggccga cgaacggctc
      421 gccctgcagg tcttcgtccc ggccgggtgc ttcgccgacg aacacaattg cggcctgggg
      481 attgccggag ccgaaaacta agttcgttcg gctggcgcct aaagggcacc tttgacaatt
      541 tttgattttg tcatacaact catagagcgt ctgcgcttcc agtccgggaa tcgcctcacc
      601 cgtcggggaa aagcgctgcg ccggcggaag cgcctcgtcg aagtaaattt ccgaaaaccc
      661 aaattcggtt tgctgcttga gcgcggcctg cgctaatccg aataggttgg cctggtctct
      721 ctcttgggcc actgggtcga cttccgttcc ctagctttaa ggaggtttac cacttcggcg
      781 acaatccggt cggctacctg acgtttgcgt aaaaggggca agggcacggt ctcgccatcg
      841 cggaaaagta aagtgacgcg gttggtttcg acttcaaatc ccgcgcccgg ctccagggga
      901 ttgttgacga ccatgagatc gacgtttttg gcttgcagtt tctcgcgcgc ctgtttgact
      961 tcctgttcgg tttccagcgc aaagccgacc agaatttgct tgtccttctc cctgcccatc
     1021 tcagccaaaa tatcgaccgt cggggccagc gtaacggtta aagttttctt ggttttcttc
     1081 aacttatgcg caacgggctt ttccggcgcg tagtcggcca ccgcggcggc catcaagagc
     1141 acgtcacagg catcgaatcg atcggcgacc atagcataca tttcttgcgc gctgctcacg
     1201 gatacggagg tcactccagc tggcaaagca atggaaacgg ggccgtgaac gagggttaca
     1261 ttagccccgg tgtcgcgcgc cgcttcagcc agggcaatgc ccatccgacc cgaggaacgg
     1321 ttcgagaggt agcgcaccgg gtcgagcggc tcttcggtcc gcccggcggt gactaacacc
     1381 ttcttccctt taagcggccc ttgaccgcca accaattctt cgatcctggc caggatgacc
     1441 tcgacttcgg gcagccgccc cgggccaaaa tcgccggagg ccaaggggcc gatgtccggg
     1501 tcgagaacgt ggacaccttc ttccttcagc cggacaatat tcttctgaac ctgcggattg
     1561 gcccacattc catcgtgcat agcgggcacg agaagcaccg gagcgcgggt ggcaatgaga
     1621 acattagtga ggagatcatc agccaggccg cccgccactt tgcctataac attagctgta
     1681 cagggcgcca cgacgaccaa ttccggccag tcggccagtg taatgtggtg ggtgccgaca
     1741 aattctcttt cggggaacat ctcaatgccc accggccgct cggagagagt ttccatagtc
     1801 aagggagtga tgaactgggt agccgatttg gtcatcacta ccctaacttc gactcccgcc
     1861 tgcctgagcc gccgcaccag ataggccgct ttataggcgg caatcccgcc ggtgacaccc
     1921 accagaattt ttttgccggt taacatcgag acggtactcc tgaaccagcg ccaaatctaa
     1981 cggaaaatag tgaaagtgca accagaccca taatcatcat cgacaaatct accggaaggg
     2041 gacagccctc agtgccgaaa atgccggatg ccggtgaaga ccatagcgat gctagattga
     2101 tcacaggcgg caatcacttc cgggtcggct ttggagccgc cgggctggat gatagcggca
     2161 attccggccc gggctgccag gtcgatgctg tcaggcatcg gaaagaaagc gtctgacgcc
     2221 agaaccgcgc ctttagcccg gtcgccggct ttgtccagag ccagccgcac tgcatcgact
     2281 cgggaggtca caccgccgcc gatgccgacc gttttggttt tctgcgcgat gacgatggcg
     2341 ttcgatttga cctgcttgac tgcgtggaag gcgaagagga gtgatgcaat ctgatccgcc
     2401 gtcggcgctt tctgcgtgac ggtttttagg tctggcgtac cgacccgata ggtatcaggc
     2461 gtttgaacta aaagaccacc tgagattacc cgtgcaaccc gacgaggcag tgcattcttc
     2521 tgaagcatct ccaggcaagc aactagacgg cgatttttct ttttctgcaa aagttccagc
     2581 acttctggag cataatcggg ggcgagaaca cactcaacaa acggcgtggc gtcgattcgt
     2641 tgggctgtct cgacatcaac tttccggttc aatccgatga tacagccgta cgccgagagc
     2701 ggatcgcaat cgtacgcatc ctgatacgct tgcgccagcg aattatcaca ggccaaaccg
     2761 cagggattgg catgcttgac gatgaccgcg cagggctcgc tgaaatcgag aacagtcgag
     2821 agtgcaaagt cgaggtccat aaggttgttg taggaaggtt ccttgccgga gagaagcttg
     2881 gcggtcgcca gcgacggtcc ccggtagtca gatttccggt acagcgccgc ctgctggtgg
     2941 gggttttcgc cgtagcgcaa ggcctggact ctggcgaaac ttccgctcag ttgctccgga
     3001 aaaatttcgc cggttttgct ctgccgggaa aggtactcgg ttatggcgcc gtcgtacgcg
     3061 gcggtgtggg caaaggccct ctgcgcgaat tgctgtctca gagacaggct gaccgatccc
     3121 tgattttttt ggagttcatc tttgagcgcc gggtagtcag ccggactggt gacgaccgtg
     3181 acgaaccggt ggttcttggc cgccgcgcgg accaacgtcg ggccgccgat gtcgatctgc
     3241 tctat
//
LOCUS       DBNGZA010000082        20798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1711537, whole genome shotgun sequence.
ACCESSION   DBNGZA010000082 DBNGZA010000000
VERSION     DBNGZA010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 20798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20798
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1711537"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC16FU_01710"
     CDS             <1..432
                     /locus_tag="AC16FU_01710"
                     /EC_number="4.2.1.136"
                     /inference="COORDINATES: protein motif:HMM:NF013425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0046496 - nicotinamide nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR2993126.1"
                     /translation="SALKNRTHPTIVTPHVGELARLLSESPEELNRNRIESARQAARE
                     LNCIVVFKGAPTLIGEPSGNVYLNPTGNSGMATAGSGDVLTGAIAGIWAQGAKPVQAA
                     CAGVFLHGLAGDLSRDAKNEYSLIAGDILDHLPQTISSLFS"
     gene            497..1783
                     /locus_tag="AC16FU_01715"
     CDS             497..1783
                     /locus_tag="AC16FU_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR2993127.1"
                     /translation="MRVQPLKFVVPVLLALVVLLSLCEPASAQKLLIPMDLAQADHLK
                     AYGIAYWCLQNAHNVEWLLNYRGGSFLTDGWQALADLCRLRGVTFTPIDAAGAGQIYQ
                     TIEQGNMDVVLLEKSPKLAVYVPANREPWDDAVRLALDYAEIPYTTLWDTEVLRGDLA
                     KYDWLHLHHEDFTGQYGKFYGAYRNALWYREEVATNEALAHALGFRKVSEEKKAVART
                     IKDYVTSGGFVFAMCSAPETMEIALAAEKDDIVAQEFDGDPPDPDYQKKLDYSQCLAF
                     ENFVVYPDALDNRRSNIDTITDRLRYIKNQETDYFTLFDFSAKLDPVPTMLVQDHVNA
                     VAGFMGLTTAFRKSLLKKYVTILAEVEGEDEVRYIHGNYGRGTFTFYSGHDPEDYQHF
                     INDPPTDLSLHKNSPGYRLILNNILFPAARKKERKT"
     gene            complement(1931..2536)
                     /locus_tag="AC16FU_01720"
     CDS             complement(1931..2536)
                     /locus_tag="AC16FU_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2993128.1"
                     /translation="MTSQRKVKVLVVTADQPQAAELGQALVQKGYQAQVTGKAATALA
                     KIHNENPHIVFVDQTLPDRSGLDFLREIRKHNTDLCVIMFTDQPTVEQAVASFKLGVF
                     DYLKKPSKPADLWPVVERAVVERGLVTDLAERVNLKIGERLRALRLKKDLTLQQVANR
                     ADVSVSLISQIERAQSSASLETLTKLASALGAKLASLFEGL"
     gene            complement(2611..2967)
                     /locus_tag="AC16FU_01725"
     CDS             complement(2611..2967)
                     /locus_tag="AC16FU_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR2993129.1"
                     /translation="MTSDLQYVRVAALADISEGEGKRVRVGLAVIVLFKISGDLFAVE
                     GLCRHMKAPLEFAQVQPNQTITCSWHGWQYDLTSGQCLTTSWAKLKTYPVKIENGQVW
                     VSVPGPVEPEKSSAAL"
     gene            3078..3479
                     /locus_tag="AC16FU_01730"
     CDS             3078..3479
                     /locus_tag="AC16FU_01730"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR2993130.1"
                     /translation="MLQVAAAVIVQNGKILIGQRKKTDRFPLKWEFPGGKVQSKESPE
                     TGLLRELSEELGIEAQIGNKLTEVTYAYPNFGPVRIQFFTVTRYHGTLENRAYEQIAW
                     VNPAQLPDYDFLEADRSLIRELARSTVAGGR"
     gene            3617..4399
                     /locus_tag="AC16FU_01735"
     CDS             3617..4399
                     /locus_tag="AC16FU_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD family protein"
                     /protein_id="HIR2993131.1"
                     /translation="MMLQQTQVERVLPKYREFLKKFPTLTSLAKAPVAAVIRCWCGLG
                     YNQRAVYLHRAARLLIERNGGKFPRVPVELQKFPGVGPYPAGAVAIFACNRDVVMLDT
                     NIRRVLTRVFAGQRPVSEKKLHKLAFRLLPAGRSKIWHSALMDLGALLCTTRNPKCPL
                     CSVQRDCLWYRESQRKPRPASRPARRKQPPFRNSTRFWRGRIVAALRELSNGRKFSLF
                     QLGKRIKPNFKTADLPWLKRLATALVRDGLATQSGRQISLPT"
     gene            complement(4536..5390)
                     /locus_tag="AC16FU_01740"
     CDS             complement(4536..5390)
                     /locus_tag="AC16FU_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2993132.1"
                     /translation="MKISQIFGLSVCFLPASAGCFTSAQAEPADSVHANQTAGAAQNS
                     TLKALDHLVSEFWTDTKSVVFTPLHWQKKDWVVCASAGAVTVGLLATDHDVEAEISRR
                     RSNLSDDLARWVTPLGSREGASLAMGGLYVGGIVFRHPTSRRAAWNSFKAVAIAQGIT
                     QVLKHGLNRRRPGALQNPGHFDGPTWHSPETGLSFPSGHTTTAFALASAISRTYRKPY
                     VTIPAFTLAGTVALARLNDFVHYPADVFFAAAMATWVGQVVSREKGERKVQVGLLSGN
                     RAGLVVRF"
     gene            complement(5422..6168)
                     /locus_tag="AC16FU_01745"
     CDS             complement(5422..6168)
                     /locus_tag="AC16FU_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993133.1"
                     /translation="MPLFPRRSVRTYSGVVLFFLLLTPTVAFAHREDYLNKTFVYQTL
                     DHRELELEYWAHYGSTSVSRSVLNTWQHTASFEYGILEHWMVEAFSTFQHLPGRNLGY
                     VATHVETRVRLFDEGKKFLDPAFSLEYSHSNAEGVSIHSLEPTIVLSKDLQRLNFTLD
                     FSLEQQLNAGHETEFGYAIATRYDTKHLLRIGAEAQGVFGHAAPHYLVPQVHLVLPAE
                     VTAKVGVGFRFSGRGEKIFLQLLTELGLEL"
     gene            complement(6215..6598)
                     /locus_tag="AC16FU_01750"
     CDS             complement(6215..6598)
                     /locus_tag="AC16FU_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2993134.1"
                     /translation="MKSVGAVLIYVKEIERVKAFLRDSLGLHLFDSKGDWAGFKLGEV
                     LIELFGPQKSGLDVGVPGRSGIKDREQQPLRLSFKVDDIEAEVARLKSKGVRLVTPVG
                     EESWGKYVWFADPEGNQYQLWKPAK"
     gene            complement(6636..7535)
                     /locus_tag="AC16FU_01755"
     CDS             complement(6636..7535)
                     /locus_tag="AC16FU_01755"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR2993135.1"
                     /translation="MNILVAGGTGFVGSEIARQALARGHTMRCLARGTRDVSGTVREL
                     KRLGAEICHGNLAEPASLTSAFAGVNVLVHAIGIIRERKEQTFEKLVVTGTRNLVAEA
                     KRAGVRQIIYISAAGTRENARSTYHRTKWQAEQIIIQSGIPYTVFRPSVIFGPRDAFI
                     NLLAKLIRYNPVVPVIGSGLSKLQPVSVKDVAACAVKSLADPRAQNQIFEIGGPQAYT
                     YNELLDVVAKVMLKKRMKIHLPLALMRPVVRLLEIMLTNPPVTSEQLIMLQEDNVCDN
                     RKLIGTFGIALLPLEAGIREYLT"
     gene            7733..8737
                     /locus_tag="AC16FU_01760"
     CDS             7733..8737
                     /locus_tag="AC16FU_01760"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR2993136.1"
                     /translation="MERTTTISGTGHAVPENVLTNQDLEKMVDTSDEWIIERTGIRQR
                     RIGNATTTASSLATAAAERALTMAKVRPEELDLILVATVTGDMPFPSTACLVQKNLNA
                     LKAVPFDLSAGCTGFIYGLATADAFIRAGVYQKVLLVGVEILTKITDWTDRNTCVLFG
                     DGAGAVVLTAKSHVATHHNGQAGLLGTFLAGDGRLGNLLMMPGGGSLYPATHASVDQK
                     LHFLKMSGNDVFKLAVTEMGEAATTVLEKTGIRPEQIDLLVPHQANLRIITATAKRLK
                     LPMEKVYVNIDRYGNTSSASIPIALDEIRRSNRYHPNQLLLLVAFGAGFTWGSALVRW
                     "
     gene            complement(8816..10156)
                     /locus_tag="AC16FU_01765"
     CDS             complement(8816..10156)
                     /locus_tag="AC16FU_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HIR2993137.1"
                     /translation="MKFFSNLPVTLSNFSQKWVPGSFIIALLLTLFTIAMALVVAHQP
                     PLKVIEFWGDGFWELLSFAMQMCLIILTGYIVASSPPVDRLFRAAAELARGPKSAILL
                     MTAVSLLLSWLHWGLSMVGAAIFVRHLARRDLEVDYRLLVACAYLGMGCTWHAGLSAS
                     APVLVATPGHFLEKAVGIIPTSLTIFSPFNLLLVGFIFAFLCFFTPLLHPKPEETRRV
                     DPSLLEQFQQQPVFSAPKKDSLSVILEYSPLVNLIFAAAGLVWLLVHFSGKRFDLTLN
                     VLNFTFLFLGILLHWTPARFLRSAEEGGKLIFGVVLQFPFYAGMYGIIKSSGLADIVG
                     NWFVSISSQKTYPAVIYWYSGLINYFVPSGGSKWAIEAPYILAAAKSLAVPAHKVVVA
                     YAWGDMATDLIQPFWAIPLLTVARLEFKDIMGYLMVIFLVYLFLVSAAFLLFPL"
     gene            complement(10172..10948)
                     /locus_tag="AC16FU_01770"
     CDS             complement(10172..10948)
                     /locus_tag="AC16FU_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR2993138.1"
                     /translation="MQIDLFFTPAEVSEADLANKTAVVIDVLRASTTVAWALFNGARE
                     IIPVDTIGAASELKQKLGKDHVLLAGEREGVKIDGFDLGNSPSEYQAPVVKEKIVVYC
                     STNGSKAMLKAGQAGLCLIGGLVNLSAVLEQVLASGRDVVLVCSGTNGKFSLEDAVCG
                     GFFVSELRRKLRQDCAENDAAGVALSIYHQLGKDLLATLKSSAHGKYLVRLGFEQDLQ
                     TAAEVDSLSVVPVHEAGRFVWRAEKAEPAAAPREFPVGAR"
     gene            complement(10970..12067)
                     /gene="gcvT"
                     /locus_tag="AC16FU_01775"
     CDS             complement(10970..12067)
                     /gene="gcvT"
                     /locus_tag="AC16FU_01775"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR2993139.1"
                     /translation="MADELKKTPFYNLHVQSGAKIVPFAGFLMPVQYRGITEEHRSVR
                     SSVGLFDLSHMGEFVVTGQDALAFLQKMTVNDVAALAPCQVQYSCMCYPEGGIVDDLL
                     VYRLPDHYWLVVNASNIAKDFGWLKEHLSGDVQLRDISDQTGLLAIQGPKAEPLLAKL
                     TDYPLATLKFYWAAEGIIAGEKVLFSRTGYTGEDGFEIYCKPEQAEKLWQAAMEAGDE
                     FDVEPIGLGARDSLRLEMKYMLYGNDIDRTTNPIEAGLGWIVKTDKGDFIGREVIVQA
                     RQQGVRKLVAFEMTEKAIPRHGYEINADGQRIGQVTSGTFSPSLEKGIGLGYVPKQFA
                     APGQGFGVAIRDRIVPAVVVQPPFWKQYSHK"
     gene            12228..13313
                     /gene="ychF"
                     /locus_tag="AC16FU_01780"
     CDS             12228..13313
                     /gene="ychF"
                     /locus_tag="AC16FU_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR2993140.1"
                     /translation="MQIGIIGLPFVGKTTVFNAVTAQARAVTSHGPAEKVHHGVIKVP
                     DSRLDFVAGLFHPRRVTPAEIEYLDVAGITGETHEKKTVEHEIPPALHEVAALAQVVR
                     TFSDPAILHISGSVDPRRDIEKLDLEIFLADLVTLEKRMERVKHGLKMGKKEFQAEFD
                     LLSRCQKALEQNRPLRDLDLTSEESHQLSGFGLLSIKPMLYLFNIDETDINGGPEVVA
                     PFADLTRHRHTIVYPLCAKIEMELAQLPDGDRPAFMEAYGIKESALDSLIHLSYQLLG
                     LISFFTASEPEVKAWAIPRGTTALKAAGTVHSDMERGFIRAEVVAVATLRELGDLHKV
                     KEKGLLRLEGKEYPVQDGDLIHFRFHV"
     gene            13511..14869
                     /locus_tag="AC16FU_01785"
     CDS             13511..14869
                     /locus_tag="AC16FU_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993141.1"
                     /translation="MQDRRLIQNLMVTGAIFCLLGVATLAFGQATGEKAKQQRLSPAG
                     FDAVISAHSQQMLREGKRIFRFDTFGDEAFWGDALKLHQAIEGEKQGGVGPGVSPKTA
                     LSVGLKVDVDALPPALVEQLKAGKVDLDDPATTLALLKLNAVVGVTGFFNEKGNLRSI
                     GIQCASCHSTVDNSFAPGIGHRLDGWANRDLNVGAIVNLAPDLGVVTSLLGVDEATVR
                     KVLQSWGPGKFDAELFMDGKALRPDGKSAATLIPPAFGLAGVNLHTWTGWGSVTHWNA
                     FVANLEMHGKGTFFDPRLNDPAQFPVAAKAGFGNVRNTPDLVSSKLAALHFYQLAIPA
                     PAPLQGSFNAGAAKRGEAVFNGKAKCAGCHVPPLFTEPGWNMHTAAEVGIDDFQANRA
                     PDKRYRTAPLKGLWSHQNGGFFHDGRFANLKEVIEHYNTFLGLGLTEPEKSDLIEYLK
                     SL"
     gene            15026..15427
                     /locus_tag="AC16FU_01790"
     CDS             15026..15427
                     /locus_tag="AC16FU_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR2993142.1"
                     /translation="MYDGYNATITDHLKNPRNAGDLPGADVVATDKNDYCGDIMRLSL
                     KVVDGIIADLRFKTFGCGISIAAGSMMTVLAQGKTVSEVQQVTENQIASALGGLAPGK
                     IHGAQLAAAVLQKAVAEYLAKSANGSRGAGR"
     gene            15387..16181
                     /locus_tag="AC16FU_01795"
     CDS             15387..16181
                     /locus_tag="AC16FU_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2993143.1"
                     /translation="MRKAPTAAGAQAGDMNPLPLTHPSPTVETSLDLVRAVPFFRTLA
                     DADAAPLIGFFHERKFAKGDYLFWEGDAADWMYVIRSGKVRMFKSSPNGKEINLEIMA
                     PGEFCGGGAIYVDVQPASAQALTETVACGIRKTDFQEVLTRHPRLAQEVIAYLGQKLM
                     SAHDMILSLITSRVENRVASLLVRLAEHHGTKTPEGVRINIALTRQNIADVIGSTVET
                     TIRVMRRFEREKILGTREGRIMIFQLDRLQKMQFAQESGPSKKQRK"
     gene            16238..17959
                     /locus_tag="AC16FU_01800"
     CDS             16238..17959
                     /locus_tag="AC16FU_01800"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR2993144.1"
                     /translation="MTSNQISPVIPAHLKPPVRPGEFVSGLAAASENRIDVGVLFVGA
                     GPAGLGGAIRLAQLLEQAPKVKEALGEVPIAVIEKGKYPGAHLVSGAVVNPVVLRKLF
                     PDLKDGDFPFFGRVEKERVYFLTARQAFRAPFLPPTMRNHGNFVASLSRLGKWLAEKA
                     EAAGVTILSETVGMKLLVKNGAVCGVQTDDKGRDREGNPLPNFQPGSEIIAKTTVLGE
                     GTTGHLSQAAVEHFRLTRPNPQIYALGVKEIWEIPKPLDSVIHTMGWPLSSSKKAREF
                     GGSFIYPMGRNMVSIGLVVGMDYADAALSVHDLLQEFKTHPLIWNILSGGKRVGWGAK
                     AIPEGGFYSLPEPLHAPGALFVGDAAGFVNVPALKGIHYALWSGVLAAEAIFEALQNN
                     RNPATPGVLMAYDLKIKNSFIWRDLYRVRNMRQAFGYGFFSGAALAGMMTLTGGLFPG
                     WRFKTHLDSEHPVSFGESRDGHTYPKPDNVLTFDKLSSVYASGNRSRDSQPNHLRVTI
                     DVPEAIGETWIHMCPAHVYEWENDGSGQKKIRINPSNCIQCGAISAKGGRLTPPEGGS
                     GPEYTLT"
     gene            18040..18807
                     /locus_tag="AC16FU_01805"
     CDS             18040..18807
                     /locus_tag="AC16FU_01805"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIR2993145.1"
                     /translation="MLLKDKVALVTGASRGLGAAIATALGQAGAKIVVNYFKNKDKAE
                     QVAQKIKSSGSDAIAIYADVRNRKAVEALVAEATKKFGRIDILVNSALYNYSFDPVRR
                     KTFLQINWQDYQEQINGTLKGAFNCCQAVLPQMIQRKSGKIIQILTNLVNNPVVQYHD
                     YTTAKAALVGFSRNLAAELGPYGITVNMIAPGLTVPTDAAAATTEEVKQLVASITPLR
                     RVGSPEEVAQVVVAWASDQMNWATGQYITADGGLVMA"
     gene            complement(18935..20176)
                     /locus_tag="AC16FU_01810"
     CDS             complement(18935..20176)
                     /locus_tag="AC16FU_01810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2993146.1"
                     /translation="MTNSEINRPQATGVPMVFRLFLLFGILVIALFFIWYTQNVIAKL
                     KEDQAKVVDMYARLWQLVTQEPTTGPAVSFIFEEIIQKSNIPIIITDSGGSIQFWRGL
                     EQVPEKDRSPANLARVHQMLEKFDHQRGPLPIRYGDRIIYYFHYGDPKLVRQLQQMPW
                     ILLSLFGLFLVVAFIGYRNIKRSEQRFIWVGMAKETAHQLGTPLTSLLGWLEILKSHT
                     AETGQAENPSLAEIAERMEGDIVRLQRVAQRFGQIGSLPELKPVDLNALVHEAASYFR
                     TRLPGSGQGVVIKENLKELPPVEANPELLSWVIENLLKNALEAVDASTGHIEVTSRMS
                     KDEKTVCITVTDNGKGIPSREQKKVFHAGYTTKKRGWGLGLTLAKRIVDEYHKGKLAL
                     VESIPNQRTEFQISLPLRNPS"
     gene            complement(20177..>20798)
                     /gene="serS"
                     /locus_tag="AC16FU_01815"
     CDS             complement(20177..>20798)
                     /gene="serS"
                     /locus_tag="AC16FU_01815"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR2993147.1"
                     /translation="YRLEQDDLWLVPTAEVPVTNLFRDEILEADGLPVYRAAYTPCFR
                     REAGSYGKETRGIIRVHQFDKVELVKIVRPEHSYAELEKLVTDAEEVLQLLGLPYRVR
                     LLSTGDLSFAAAKCYDLEVWAPGVESFLEVSSCSNYEAFQARRMNVRFRPAPNAKPDY
                     PHTLNGSGLALPRTLAALLENYQTEKGTVRIPDVLQPYLGGLAEIT"
BASE COUNT         4637 a         6177 c         5698 g         4286 t
ORIGIN      
        1 tcggctctga aaaatcgcac acatccgacc atcgtcaccc cccacgtggg ggaactggcc
       61 cgcctcttga gtgaatcgcc ggaggaattg aaccgcaatc gaatcgaaag cgcgcgccag
      121 gccgcccgcg aattaaattg cattgtcgtc ttcaaaggtg cgccgacttt aatcggggaa
      181 ccctctggaa atgtctacct gaaccccacc ggcaactccg gcatggctac cgccgggtca
      241 ggtgacgtgc tgacgggagc catcgccggc atttgggcgc agggagccaa accggttcaa
      301 gcggcctgtg ccggtgtgtt cctccacggc ttagccggcg atctgagccg tgatgcaaaa
      361 aacgagtaca gtctcattgc gggggatatt ctcgaccacc tgccccagac gatttcgtca
      421 cttttctctt gaattttcca gtacgactcc ggtaaaattc tttcagaaag actgaattct
      481 gccgtataac caaaagatgc gcgtgcagcc attaaaattc gtagtgcctg tacttttggc
      541 tctcgttgtt ctcctctccc tgtgcgaacc cgcctcagcc cagaaactct taatcccgat
      601 ggacctcgca caggccgacc acctgaaggc ctacggcatt gcctactggt gtttacagaa
      661 cgcccacaat gtcgagtggc tattgaatta ccgcggcggt tcgtttttga ccgacggctg
      721 gcaggcgtta gcagacctct gccgcctgcg gggggtcact tttacaccca tcgacgccgc
      781 cggagccgga cagatttacc agaccattga acagggcaac atggacgtgg tgctgttgga
      841 aaaatcaccc aaattggccg tctacgtgcc tgccaaccgc gagccctggg acgacgcggt
      901 acgcctagct ttggactatg ccgaaattcc gtacaccacc ttgtgggaca ccgaagttct
      961 acgcggcgac ctggccaagt acgactggct ccacctgcac cacgaggact tcaccggcca
     1021 gtacgggaag ttctacggcg cttaccggaa cgcgctctgg taccgcgagg aagtggccac
     1081 gaacgaagcg ttggcccatg ctttgggctt ccgcaaggtt tccgaagaga aaaaagcggt
     1141 cgcccggacc atcaaggatt acgtcacctc cggcggtttc gtcttcgcga tgtgctccgc
     1201 gccggaaacc atggagatcg ccctggccgc cgaaaaggac gacattgttg cccaagagtt
     1261 cgacggcgac ccgcccgacc cggattacca gaaaaaactc gactactccc agtgcctggc
     1321 ttttgagaac ttcgtggtct accccgatgc cttggacaac cgccgttcca acatcgatac
     1381 catcaccgac cgcctgcgct acatcaaaaa ccaggaaacc gattacttca ccctgttcga
     1441 cttttcagcc aaactcgacc cggtgccgac gatgttagtc caggaccacg tcaacgcggt
     1501 ggccggattt atgggattga ccacggcgtt ccgcaagtca cttttgaaga agtatgtgac
     1561 catcttggcc gaagtggaag gggaagatga agtgcgctac atccacggca actacggccg
     1621 cggcactttc actttctact ccggccacga cccggaagac taccagcact tcatcaacga
     1681 cccgccgacg gacctctcat tgcacaagaa ctcccccggc taccggctga ttttgaacaa
     1741 catcttgttc ccggcagcgc gcaaaaaaga gcgcaaaacc taaaaaacca agagatcaac
     1801 ccctcttacg ttgtagcccc cctctcttca gcttgagtcc cgcctcgggc gaatcgaagg
     1861 ccagaacggg gaaattatag aataagaaaa tgcgcttgta ggaacgaacc gctataaccc
     1921 ggcaactgac ttacaatccc tcgaataagc tcgctaactt cgctcccagc gcgctcgcta
     1981 gcttggtcaa ggtttccaga gaggccgaac tctgcgctcg ctcgatctgg gaaatcaaac
     2041 tgaccgatac gtcggcccgg ttggccacct gctgcaaagt caggtccttt ttcaagcgca
     2101 gcgcccggag ccgttcgccg attttcagat ttacgcgctc ggccaaatcg gtcaccagcc
     2161 cgcgttcgac cactgctcgc tccaccaccg gccaaaggtc agctggcttg gacggtttct
     2221 tgaggtagtc aaagactccc aacttgaaac tggccaccgc ctgctccacc gttggctgat
     2281 cagtaaacat aatgacgcaa agatcggtat tgtgcttccg gatttcccgc aggaagtcaa
     2341 gcccgcttct atccggcagg gtctggtcga cgaagacgat gtgcggattt tcgttgtgga
     2401 ttttggcgag cgccgtggcg gccttgccgg tcacctgagc ctggtaaccc ttttgcacca
     2461 gggcttgtcc taattctgcc gcctgcggct ggtcggcagt caccaccagc accttcactt
     2521 tgcgttggct ggtcataaaa cacctctttt tctttctgcc cgtagagtat acgtgaggta
     2581 ttcaactacg ttgtaaaaat aggagggagc ttaaagggca gctgaacttt tttccggttc
     2641 gacgggtcct ggcaccgaga cccagacctg cccgttttcg attttcaccg ggtaggtttt
     2701 gagcttggcc caggacgtag taaggcactg accggaggta agatcatact gccacccgtg
     2761 ccaggagcag gtaatggttt ggttgggttg cacctgcgcg aattccagag gcgccttcat
     2821 atggcggcaa agtccttcca cggcaaaaag atctccgctg attttgaaaa gaacaataac
     2881 cgccagaccc acgcgcaccc gtttcccctc cccttcggag atatccgcaa gtgccgccac
     2941 ccggacgtac tggagatcgc tcgtcatttc ttgcgagact accggcgcag ctgatttttg
     3001 tcaaggtcgg cccgtctgtt ccgatctccg tgatgggctc ccttgtacca tcgccacaga
     3061 atttctatgt ttaagaaatg ctccaggtcg ccgctgcggt catcgtccaa aatggaaaaa
     3121 ttttgatcgg ccaacgcaaa aaaaccgacc gctttccgct caaatgggaa tttcccggcg
     3181 gcaaagtaca gtctaaggag tcaccggaaa ccggtctcct gcgggaactc agtgaagaat
     3241 tagggattga agcgcagatc ggcaacaaac taaccgaagt gacctacgcc tacccgaact
     3301 ttggcccggt gcggattcag tttttcaccg tcacacggta ccatgggacc ctggagaacc
     3361 gggcgtacga acagatcgcc tgggtcaatc ctgcccaact gccggactac gactttcttg
     3421 aggccgaccg atcgttgatc cgggaactgg cacgcagtac ggtagccggg ggtagataga
     3481 aagagcacct gtgaaagcaa tttccacgaa aaaaaagaac agattccgaa aaaacttgcg
     3541 gcgctggttt gtccgaaata gacgcgacct cccctggcgc cgaacccgga atccataccg
     3601 cattctggtt tccgaaatga tgctccagca aactcaggtg gaacgcgtcc tgccgaagta
     3661 ccgggagttc ctcaaaaaat ttccgacgct gacttctctg gcaaaagcgc cggttgctgc
     3721 agtgattaga tgctggtgcg gcttggggta caaccagcgg gcggtctacc tccaccgagc
     3781 cgctcgactg ttaatcgagc gaaacggcgg aaaatttccc cgcgtgcctg tcgaattaca
     3841 gaaattcccc ggcgttggtc cctatcccgc cggtgccgtt gctatttttg cctgcaatcg
     3901 agatgtcgtg atgctggaca ccaacatccg gcgggtgctg accagagtgt ttgccggtca
     3961 gcgacctgta tccgaaaaaa aactccataa actggctttc cggctcttgc ccgctggccg
     4021 gagcaaaatc tggcacagcg cccttatgga tctgggtgct ttactctgca ccacccgaaa
     4081 tccaaagtgc cctctctgtt cggtacaacg tgattgcctt tggtatcggg aatctcagcg
     4141 aaaacctcgg cccgcgagcc gtcccgcacg acgaaaacaa ccgccatttc gcaattccac
     4201 ccgcttctgg cgcggacgga ttgtggctgc actgcgggaa ctttccaatg gccgaaagtt
     4261 ttcgcttttt cagttgggga aacggatcaa gccgaatttc aaaaccgccg acctgccctg
     4321 gctcaaacga ctcgctaccg ctttagtccg cgacgggctg gcaacccaga gcggccggca
     4381 aatctcgctc ccgacgtaat tgcagccacg ctcttttgag cgcggtgaaa gttgaggcat
     4441 tgggaataat aatcgcacag cagatgaagc agattgagca gatcttctct gtcggatatg
     4501 cttcatccgc tccctctgct gcgaatgcct tctttctaaa accgcactac caaccctgct
     4561 cgatttccgg atagcaaccc tacctgtact ttcctttccc ctttttcccg gctgaccacc
     4621 tgccccaccc acgtagccat cgccgcggcg aagaatacgt ccgccgggta gtgcacaaaa
     4681 tcattcaagc gtgccagagc caccgtaccg gccaatgtga acgcgggaat cgtgacgtat
     4741 ggcttccggt acgtcctact tatagccgaa gccagggcaa acgccgttgt ggtatggccg
     4801 gaaggaaagg aaagtccggt ttcaggtgaa tgccaggtcg ggccgtcaaa gtgcccgggg
     4861 ttctggagcg cgcccggacg ccggcgattc aatccgtgct tgaggacctg cgtgattccc
     4921 tgcgctatgg ccaccgcttt gaagctgttc cacgccgcgc gccgtgaggt tggatgccgg
     4981 aaaacgatgc cgccgacgta aagcccgccc attgccagac tggcgccctc gcggctgccc
     5041 aaaggcgtta cccagcgcgc cagatcatcg gagaggtttg agcggcgccg ggaaatttcc
     5101 gcttcaacgt cgtgatcggt tgccagaagg ccgacggtaa ctgcgccggc gctggcgcaa
     5161 accacccaat ccttcttctg ccagtggagc ggtgtgaaaa cgaccgattt cgtgtcggtc
     5221 cagaattctg atacaagatg gtcgagggct ttcagcgtgg agttctgtgc ggcacccgcc
     5281 gtctgattcg cgtggacgga atccgccggc tcagcctgag ccgaagtaaa gcaacccgcg
     5341 ctggccggga ggaaacaaac cgaaagcccg aaaatctgtg agattttcaa agcgcttctg
     5401 cggggctgac cggttcagtc ttcacaattc caggccgagt tccgtcaaaa gctgcaggaa
     5461 aattttttcg ccccggccgg agaaccggaa accgacgccg actttggccg tcacttcagc
     5521 cggcaaaacg aggtggacct gcggcacaag atagtgggga gcagcgtgcc cgaacactcc
     5581 ctgggcttcg gcgccaatgc gcaaaagatg tttggtatcg tatcgcgtcg cgatggcata
     5641 gccaaactcg gtttcgtgcc cggcgtttaa ctgctgctcc agcgaaaagt cgagggtgaa
     5701 attcaaccgc tggagatcct tagaaaggac tatagttggt tccagactgt ggatagaaac
     5761 cccttctgca ttggagtggc tatactccaa tgaaaaggcc ggatcgagaa acttctttcc
     5821 ttcatcaaaa agtcgaaccc gtgtctcaac atgcgtggct acgtagccca gattcctgcc
     5881 gggaaggtgt tgaaaagtgg aaaaagcttc gaccatccag tgctcgagaa tgccgtactc
     5941 gaaagatgcg gtgtgctgcc aggtattcag aactgaacgt gagacgctgg ttgaaccgta
     6001 gtgcgcccag tactcaagtt ccagttcccg gtggtctaag gtctggtaga cgaaggtctt
     6061 gttgaggtaa tcttcgcggt gcgcgaatgc aaccgtcgga gtcaacaata agaagaacag
     6121 aacgactcct gaatacgtgc ggactgaccg tctggggaaa aggggcatct ctatttccta
     6181 cagcatcatt ggaggaaaag tttgtgtgaa ctcttcactt cgccggtttc cagagttggt
     6241 actgatttcc ctcgggatcg gcgaaccaga cgtacttgcc ccagctttcc tctcccacgg
     6301 gtgttaccag ccgcacccct ttgcttttca gccgggccac ttcggcttcg atatcgtcca
     6361 ctttgaacga aagccgcagt ggttgctgct ctctgtcttt gatacccgac ctacccggta
     6421 cgcccacatc caagccgctc ttttgcggcc cgaacagctc aatcagaacc tcaccgagtt
     6481 tgaagccggc ccagtcgccc ttgctgtcga agagatgaag gcccagcgag tcgcgcagga
     6541 atgccttcac tctctcgatt tccttgacat aaatcaaaac ggctccgaca cttttcattg
     6601 cacctccctc gccgggggaa tcaacaacaa tactctcagg tgagatactc ccgaatgccc
     6661 gcttcgagag gtaataaggc aatcccgaac gtcccgatca gcttgcggtt gtcgcacacg
     6721 ttgtcctcct ggagcattat caactgctcg ctggtaacag gaggattggt gagcatgatt
     6781 tccaagagcc gcaccaccgg acgcatcagt gccagcggga gatgaatttt cattcttttc
     6841 ttcagcatga cttttgcaac gacgtcgagg agctcattgt aggtataggc ttggggccca
     6901 ccgatctcaa aaatctggtt ttgcgcccgc ggatcggcta gcgacttaac agcgcaggca
     6961 gcgacatctt tcaccgacac gggctgcaac ttcgataaac ccgaaccgat caccggcacc
     7021 accgggttgt accggatcag ttttgccagg agattgatga acgcatctct cgggccgaag
     7081 atcaccgatg gtcggaaaac cgtgtacggt attccgcttt gaattattat ctgttctgct
     7141 tgccacttgg ttcggtggta ggtggaacgg gcattttccc gcgtacccgc agcgctgatg
     7201 taaataatct gcctgacacc cgctcgttta gcctcagcca ccagattccg agtgccggtg
     7261 acaactaatt tttcaaaggt ttgttctttc ctttcccgaa tgatgccaat tgcatgcacc
     7321 aggacattaa caccggcaaa ggcagaagtt agggacgccg gctcggcaag gttcccgtgg
     7381 cagatttcag cgcccagccg cttcagttcc cgaaccgttc cggagacgtc gcgcgtaccg
     7441 cgcgccaggc agcgcatcgt gtgcccccgg gcgagcgctt gccgcgcgat ttccgatccc
     7501 acgaagccgg tgcccccggc gacaagtata ttcacagaga tcctcggact ctcctgccaa
     7561 gatagacgtt agaaaaaccg cgtcaatccc gcgccatgta cccaaaaatc tcttgcgtac
     7621 cgcaggctcc atttcgtatt ttataattgc gaatgcggca ggcatccttc cactacgacg
     7681 ccagggagca tgccctcagg aacctaaaga aggcctgcct gaactaaacg agatggaacg
     7741 aaccaccacc atcagcggaa ccggccacgc cgtgccggaa aacgtgttga ccaatcagga
     7801 tttggaaaag atggtcgaca cctccgacga gtggataatt gagcgcacgg gcatccgtca
     7861 gaggcgtatc ggcaacgcca ctaccactgc ctcgtcattg gcaaccgccg ctgccgaacg
     7921 agctctgacc atggccaagg tccgcccgga ggaacttgac ctcatcctgg tcgccaccgt
     7981 caccggcgac atgcccttcc cctcgaccgc ctgcctggtg cagaaaaact tgaatgccct
     8041 gaaggcggtt ccatttgacc tgtcggcggg ctgcaccggt ttcatttacg gtctggccac
     8101 tgccgacgcc ttcatccggg caggcgttta ccaaaaggtc ctcttagttg gcgtagaaat
     8161 tttgaccaag attaccgatt ggactgaccg gaacacttgt gttctgttcg gcgacggcgc
     8221 cggtgccgtg gtgctcactg ccaaaagtca tgtcgccacc caccacaacg gccaggcggg
     8281 actcctggga acctttttag ccggtgacgg ccggctcggg aatctgttaa tgatgcccgg
     8341 cggcggttcg ctctaccccg ccacgcacgc ctcggtggac cagaaactcc attttctgaa
     8401 aatgtcgggg aacgacgttt tcaaactggc cgtgacggag atgggcgagg ctgccacaac
     8461 cgtcctggaa aaaaccggca tccggccaga gcagatcgat ctgctcgttc cgcaccaggc
     8521 caatttgcgg atcattaccg ccacggccaa gcggctgaaa ctgccgatgg aaaaagtcta
     8581 cgtcaatatc gaccgctacg gcaacacctc ctcggcttcg attccgattg ccctggacga
     8641 aatccgccgc agtaaccgtt accatcccaa tcaactcttg cttctggtcg ctttcggcgc
     8701 cggcttcacc tggggttcgg ccctggtacg ctggtaagtc ctgaggactc ataaccccgc
     8761 gtgtagccac gctctcttga gcgtggctct tctaatgttg ttaagccgac ctgcttcaaa
     8821 gtgggaacaa aagaaacgcc gcgctcacca gaaagagata aaccagaaaa attaccataa
     8881 ggtatcccat gatgtctttg aattccaatc tggctacggt caaaagcgga atcgcccaga
     8941 agggctggat gagatcggtc gccatgtcgc cccaggcgta ggccaccacc accttgtgcg
     9001 ccgggacggc gagtgacttg gctgccgcga gaatgtacgg agcttcaatc gcccacttgg
     9061 acccgccgga agggacgaag tagttgatga gccccgagta ccagtagatc accgccgggt
     9121 aggttttctg gctcgaaatt gagacgaacc agttgccgac aatgtcggcc aacccggacg
     9181 acttgatgat gccgtacatg ccggcgtaga agggaaactg cagcaccacg ccgaaaatca
     9241 acttcccgcc ctcctcggcc gagcggagga agcgcgccgg cgtccagtgc agaagaattc
     9301 ccagaaagag gaaggtgaag ttgaggacgt tcaaggtgag gtcgaaccgc ttgccggaaa
     9361 aatggacgag aagccaaact aatcccgccg ctgcaaaaat gagattgacg aggggcgaat
     9421 attctaagat cacggagaga ctatcttttt tgggcgctga gaaaaccggc tgctgctgga
     9481 actgttccag aagggaggga tcaaccctcc gggtctcttc cggcttgggg tggagaagcg
     9541 gcgtgaagaa gcagaggaaa gcgaagataa aaccgaccag caataaattg aacggcgaaa
     9601 agatggtgag actcgtggga atgatgccga ccgccttttc cagaaaatgt cccggcgtgg
     9661 cgaccaagac cggtgccgag gctgatagcc cggcgtgcca ggtgcagccc atccctaagt
     9721 aggcgcaggc gaccaaaagc cggtaatcga cttcgaggtc gcgccgggcg aggtggcgca
     9781 cgaaaatcgc cgcccccacc atcgagagtc cccagtgcag ccaggagagc aaaagcgaca
     9841 cggcggtcat caagagaatg gccgacttgg ggccccgggc cagttcggct gccgcccgaa
     9901 agagccgatc caccggcggg gaagaagcga ctatgtagcc agtcagaatg atcaaacaca
     9961 tttgcatggc aaaagacaaa agttcccaga agccatcgcc ccagaactcg ataactttca
    10021 aaggcggctg gtgggcaaca accagagcca tggcaatagt gaagagggtg agcaaaagcg
    10081 caatgataaa cgagcccggc acccatttct gtgaaaaatt cgagagggtg acgggcaggt
    10141 tgctgaaaaa tttcattccg ggaaatgtag gctaccgtgc cccgacggga aattccctcg
    10201 gcgctgcggc gggctcggcc ttctccgccc gccacacgaa ccgtcccgcc tcgtgcaccg
    10261 gcaccaccga gagactatct acttccgctg ccgtctgcag gtcctgctca aaccccaggc
    10321 gcaccaggta cttcccgtgg gcggacgact tgagcgtggc caggagatct ttccccagtt
    10381 ggtggtagat ggaaagcgcc actccggccg cgtcgttttc cgcacaatcc tggcgcaatt
    10441 ttctcctcag ttcggataca aaaaacccgc cgcagaccgc gtcctccagc gaaaactttc
    10501 cgttggtgcc ggagcaaacc agcacaacgt cgcgaccgga agccaaaacc tgctcgagca
    10561 ccgcactcag gttgaccagc ccgccgatca ggcacagacc agcctgcccc gctttgagca
    10621 ttgccttgga gccgttggtg gagcagtaga ccacgatttt ttccttcacc accggtgcct
    10681 ggtattcgga cggcgaattc cccaggtcga agccatcgat tttcacgccc tcgcgctccc
    10741 cggccaaaag gacgtggtct ttccctaatt tctgctttaa ctcggaggcg gcgccgatgg
    10801 tatctaccgg aatgatttcc cgcgccccgt tgaagagcgc ccaggccacc gtggtggaag
    10861 cgcgcaaaac gtcgatcacc actgcggttt tgttggctaa atcggcctcg ctgacctcgg
    10921 ccggcgtgaa gaacaaatca atttgcattc ggacgctgct tactcctctc tacttgtgcg
    10981 aatactgctt ccagaacggc ggttggacca ccactgccgg gacaattcta tcccgtatcg
    11041 ccacgccgaa cccttggccc ggcgcggcga actgtttggg aacatatccc aaaccgatcc
    11101 ctttttcaag cgaaggcgaa aacgtgccgg aggtcacctg cccgatcctc tgcccgtcgg
    11161 cgttaatctc atacccgtgc cggggaatgg ctttctcagt catttcaaag gcaaccaatt
    11221 tgcgcactcc ctgttgccgg gcctggacaa tgacctcccg gccgatgaaa tcacccttgt
    11281 cggtcttcac gatccagccg agcccggctt cgatcggatt ggtggtccgg tcgatgtcat
    11341 tgccatagag catatacttc atttcgagcc gcagactgtc ccgcgccccc aagccgatcg
    11401 gttcgacgtc aaactcgtcc ccggcctcca tagccgcctg ccacagtttc tcggcctgct
    11461 cgggtttgca gtagatttcg aagccgtcct caccggtgta gccggtgcgc gagaacaaaa
    11521 ccttttctcc agcgatgatt ccttccgccg cccagtagaa tttgagggtc gccagaggat
    11581 agtcggtcag tttcgctaag aggggctcag cctttggtcc ttgaattgcc aaaagccccg
    11641 tctggtcact tatgtcgcgc aactgcacgt caccggaaag gtgctctttc agccagccga
    11701 aatctttggc gatgttagag gcattcacca caagccaata gtggtcgggc agccggtaga
    11761 ccaggaggtc atcgacgatc ccaccctccg ggtaacacat acaggagtac tgcacctggc
    11821 agggtgcgag cgcggccacg tcgttgaccg tcatcttctg gaggaacgcc agggcgtctt
    11881 gaccggtgac gacgaactcc cccatgtgcg agagatcgaa cagacccacc gacgaacgca
    11941 cgctgcggtg ctcctcggtg atcccgcggt actgcaccgg catgaggaat ccggcaaaag
    12001 ggacgatttt cgctccgctt tgtacgtgca ggttgtagaa aggagttttt tttagttcgt
    12061 ccgccacggc agcgcgaata atatgtagcc cggctccaat gtcaatccgc ggccatgcgc
    12121 ctgctcgcgc ggcctccggc caaatcaata ccggataacc gggttccggt aattgacttc
    12181 ccgcccaaat cacctaccat tacgatgcga aaggcggttg tgtttttatg caaatcggca
    12241 tcatcggact cccctttgtc ggtaagacca cggtcttcaa cgctgtcacc gcccaggcca
    12301 gggcggtcac ctctcatggt cctgccgaga aagtccacca cggcgtaatt aaagtgccgg
    12361 acagccggct tgactttgtt gcgggacttt tccacccccg ccgcgtgacg ccggcggaaa
    12421 tcgagtattt ggatgttgcc ggcatcactg gcgagacgca cgagaaaaaa accgttgagc
    12481 acgaaatccc gccggccctt cacgaggtcg ctgctctggc tcaggtggtg cggacctttt
    12541 ccgatccggc cattctgcac atctccggtt cggttgaccc ccgccgcgat atcgaaaaac
    12601 tcgatctcga aatcttctta gccgatctgg taactttgga gaaacggatg gagcgggtga
    12661 aacacggcct gaagatgggg aaaaaagaat ttcaagccga gtttgacctt ctctcccgct
    12721 gccagaaggc tctggagcaa aatcgacccc tgcgtgacct cgaccttacg tctgaagaat
    12781 cgcaccaact gagcggtttc ggtttgctct caatcaaacc gatgctctac ctgtttaata
    12841 tcgatgagac agacatcaac ggcggccccg aagtcgtcgc cccgttcgcc gacctgacgc
    12901 ggcaccggca caccatcgtc taccccctct gtgccaaaat agaaatggaa ctggcccaac
    12961 ttcccgacgg cgatcgccca gcctttatgg aagcctatgg gattaaagag tcggctctgg
    13021 attcgctcat tcacctctcg taccagctct tgggactcat cagtttcttt actgccagcg
    13081 agccggaggt gaaagcctgg gcgattcccc gagggacgac cgccctcaag gccgccggca
    13141 ccgttcattc cgatatggag cgcggtttca tccgcgccga agtggtcgcc gtcgccaccc
    13201 tcagagaact tggcgacctg cataaagtga aagaaaaggg gttgctgcgc cttgagggca
    13261 aagaataccc cgtgcaggat ggcgacctga ttcacttccg ctttcacgtg tagcgatacg
    13321 ctacagactg gcgcgtcaac acccactact ttattgtacc gcaaaaacat aaaagctcga
    13381 catccgaata aaattttctc ttgacaagtg atgggtcatc tattattagt tagcgacaaa
    13441 actactggtt tatattatta gcaatcgcag tcaaccgcat aaccgctatg ttggaaagga
    13501 gaccagaatc atgcaagatc gaaggctcat ccaaaacctc atggtaaccg gggcaatctt
    13561 ttgtcttctc ggtgtggcca ccctggcttt cggccaagcg accggcgaga aggccaagca
    13621 acaaaggctg tctcccgccg gatttgacgc tgtgattagc gcccactccc agcagatgct
    13681 ccgggagggt aaacgcatct ttcgcttcga cacgttcggc gatgaagcct tctggggcga
    13741 tgcgctcaaa ctgcatcagg ccatcgaagg tgaaaagcag ggaggcgtgg ggccgggtgt
    13801 gagtccaaag accgcactgt ccgttggatt gaaagtcgat gtggatgctc ttcccccggc
    13861 tctggttgaa caactgaaag ccggcaaggt cgatctcgat gatccggcca ccacgctggc
    13921 tctgctcaaa ctgaacgcag tcgtaggcgt caccggcttt ttcaacgaaa aaggcaatct
    13981 acgctcaatc ggcattcaat gcgcctcgtg ccactccacc gtggataact cgttcgctcc
    14041 cggcatcggc caccggctgg atggctgggc caatcgggac cttaatgtgg gcgccatcgt
    14101 caacttagcg cctgacttgg gtgtggtcac gagccttttg ggtgttgatg aagccacggt
    14161 cagaaaagtg ctccaaagct gggggccggg aaaattcgac gccgagcttt tcatggatgg
    14221 caaagcgctc cgtccggacg ggaagtctgc cgccaccctg atcccgcccg ctttcggtct
    14281 ggccggcgtg aacctgcaca cctggaccgg ctggggctcg gtgacgcact ggaatgcttt
    14341 tgtagccaat ctcgaaatgc acggcaaggg aaccttcttt gacccccggc tgaacgatcc
    14401 ggcccaattc ccggtggcgg cgaaagccgg tttcggcaac gtgcggaata cacctgatct
    14461 ggtctcctca aaattggccg cgctacactt ctatcaactg gctattcctg cgccagcacc
    14521 cctgcagggg agcttcaacg caggagcagc gaaacgtggg gaagcggtct tcaacggcaa
    14581 ggccaagtgc gccggttgcc acgtgccgcc gctcttcacc gagccgggct ggaatatgca
    14641 cacggccgcc gaggtgggta tcgacgattt ccaggcgaac cgggcgccgg acaaacgcta
    14701 ccggacggcg ccactgaagg gactctggag ccaccagaac ggcggtttct tccatgacgg
    14761 ccgttttgca aatctgaagg aagttattga gcactacaac acttttctcg gattagggct
    14821 gactgagcca gaaaaaagcg acctgatcga atacctgaag tcgctgtaaa ttgtaggata
    14881 aaatccagcc gaggagcata gtgcgggtgc cccttctaaa caagggcacc cgtatctgcc
    14941 cacgcaggcc ttcccgccca gacccgtact gcgccgagtt ctatgttctc atttggccaa
    15001 gtgttgtata ttgctttgac gaattatgta tgacgggtac aacgccacaa tcaccgacca
    15061 cctgaaaaac ccccgcaatg ccggtgacct tccgggggcc gacgtggtcg ccacggacaa
    15121 aaatgactac tgcggggaca tcatgcggtt gtcgctgaaa gtggtggatg gaataatcgc
    15181 cgacctgcgc ttcaaaacct tcggctgcgg catttccatt gccgcggggt cgatgatgac
    15241 cgtgctggca caagggaaaa ccgtttccga agtgcaacag gtgaccgaaa accagatcgc
    15301 ctctgccctg ggcgggctgg cgccggggaa aatacacggt gcgcagttgg ccgcggcggt
    15361 cttgcagaaa gccgtagcgg agtatcttgc gaaaagcgcc aacggcagcc ggggcgcagg
    15421 ccggtgatat gaacccgctc ccccttactc acccgtcgcc gactgttgaa accagcctcg
    15481 atctggtgcg cgccgttcct ttcttccgta ccctggccga cgcggacgcc gcaccgctca
    15541 ttggattctt ccacgaacgg aaattcgcca aaggcgatta cctcttttgg gagggcgacg
    15601 ccgccgactg gatgtacgtc attcgttccg gcaaagtgcg catgttcaaa agctccccga
    15661 acggcaagga aatcaatctc gaaattatgg ctcccggaga attctgcggc ggcggcgcca
    15721 tctacgttga tgtccagccc gcgtcggccc aggccctcac agaaaccgtg gcctgtggca
    15781 tccggaagac cgacttccag gaggttttaa cgcgccatcc gcggctggcc caggaggtca
    15841 ttgcctacct ggggcagaag ttgatgtcgg cccacgacat gatcctgagt ctcatcacca
    15901 gccgcgtgga gaaccgcgtg gcgagcctcc tggtccgcct ggccgagcac catgggacca
    15961 aaacgccgga gggcgtacgc atcaatatcg ccttgacccg gcagaacata gccgacgtca
    16021 tcggcagtac ggtggagacc accatccgcg tgatgcgccg cttcgagaga gaaaaaatcc
    16081 tgggaacccg ggaaggccga atcatgattt tccagctcga ccggttgcag aaaatgcaat
    16141 tcgcccagga gagcgggccg tcaaagaagc aaagaaaata atcccgctcc gcagatcgag
    16201 cgtcactgat gcaccagacg cgaatgctga cagctgaatg acctcaaacc aaatttcccc
    16261 agtcattccg gctcacttga aaccgcccgt ccgtcccgga gaattcgttt ccggtttggc
    16321 cgctgcgtcg gaaaatcgga tcgacgtcgg cgtgctcttc gtcggcgccg gcccggccgg
    16381 tttaggcggg gcaatccggc tggcgcaact cctggaacaa gcgcccaaag tcaaagaggc
    16441 cttaggtgaa gtccccattg cggtgattga aaaagggaaa tatcccggag cgcacctggt
    16501 ctccggcgcg gtggtcaacc cggtggtctt gcgaaaactc tttcccgatc tcaaagacgg
    16561 tgattttccc ttcttcggcc gggtggagaa agagagagtc tactttctta ccgcccgtca
    16621 ggcctttcgc gcgcctttcc tgccgccgac gatgcgcaat catggtaact ttgtcgcttc
    16681 cttaagccgt ctgggcaaat ggctggccga aaaagccgaa gcggccggtg tgacaatttt
    16741 aagcgaaacc gtcggcatga aacttctcgt gaaaaatggc gcggtctgcg gcgtccagac
    16801 tgacgacaaa ggccgcgacc gggaaggcaa cccgctgccg aatttccagc ccggctcgga
    16861 aatcatagcg aaaaccaccg ttctgggcga aggcacgacg ggacacttat cgcaggcggc
    16921 ggttgaacat tttcggctga cccggcccaa cccgcagatt tacgccttgg gtgttaagga
    16981 aatctgggaa ataccgaagc cgcttgactc agtcatacac acgatggggt ggccactttc
    17041 cagttccaag aaagcgcgcg agtttggcgg ctcattcatc tacccaatgg gccggaacat
    17101 ggtttcgatt ggcctcgtcg tagggatgga ttatgccgat gctgccttgt ccgttcacga
    17161 cctcttgcag gaattcaaaa ctcatccgtt gatctggaat attctctccg gcggcaagcg
    17221 cgtcggctgg ggagccaaag ccattcccga aggtggtttc tactccctcc cggagccgtt
    17281 gcacgcaccg ggggcgcttt tcgtcggcga tgccgccggc tttgtgaacg ttccggcact
    17341 caaggggatt cactacgccc tctggtcggg tgtcctggcc gctgaggcga tctttgaggc
    17401 gttgcaaaac aaccgcaatc ccgccactcc cggagttctg atggcgtacg acctgaaaat
    17461 caaaaacagt ttcatctggc gcgacctgta ccgcgtccgc aatatgcgcc aggcgtttgg
    17521 gtacggtttt ttctccggcg cagcgctggc gggaatgatg acgttaacgg gagggctctt
    17581 tccgggttgg cggttcaaga ctcatctcga tagcgagcat cccgtctctt ttggggagtc
    17641 gcgggacggg cacacctacc cgaaaccaga taatgtcttg accttcgaca aactttccag
    17701 cgtctacgcc agcggcaacc gcagtcggga tagccagccg aaccacctgc gggtgacaat
    17761 cgacgttccc gaagccatcg gtgaaacctg gattcatatg tgtccggccc acgtttacga
    17821 atgggaaaac gacggttcgg gccaaaagaa gattcggata aacccctcca actgcattca
    17881 gtgcggcgcc atttccgcga aaggcggtcg tttaacccct cctgaaggtg gctccgggcc
    17941 tgagtatacg ctgacgtaat tgagcctgat gataggtcgc tttgcgcgcc agcacccata
    18001 ggtatatttt aatgaccctg agaaccaact ggaggaatga tgcttttgaa ggataaagtc
    18061 gccttagtca ccggcgcatc ccgcggactg ggcgccgcta tcgccactgc tctggggcaa
    18121 gccggcgcga aaattgtcgt caactatttc aagaacaagg acaaagccga acaggtggcg
    18181 cagaaaatca aaagttctgg tagtgatgcc attgctattt acgccgacgt ccgtaaccgg
    18241 aaagcagtcg aagccttggt cgccgaagca acgaagaaat tcggcagaat tgatatcttg
    18301 gtgaatagcg ccctctacaa ctactccttc gacccggtca ggcgcaagac cttcctgcag
    18361 atcaactggc aggattacca ggagcaaatc aacggcacgc tcaagggcgc cttcaactgc
    18421 tgccaggcgg tcctgcccca gatgatccag cgcaagtcgg gaaaaatcat ccagattctc
    18481 accaacctgg tcaataaccc ggtggtgcag taccacgatt acacgacggc caaagctgcc
    18541 ttagtcggct tttcgcgcaa cttggcggcg gaactcggcc cctacggcat tacggtgaat
    18601 atgatcgccc cgggcttgac cgtgcccacc gacgccgccg cagccactac cgaagaggtc
    18661 aagcagctgg ttgcttcaat cacgccgttg cgccgggtcg gctcgccgga ggaggtagca
    18721 caggtcgtcg tcgcttgggc ctcggatcaa atgaactggg ccaccggcca gtatatcact
    18781 gcggacggcg gactggtcat ggcatgacaa agtcgggatc gggtcaggcc tgccggccta
    18841 accgctgcag aatctaaagt tcttaaaata atttctctga atttctgtag cgccgctctc
    18901 ccgagcggcg tcgcggatac gtcgtggctg gaatctatga cggattccgc agcggcaggg
    18961 aaatctgaaa ctcggtcctc tggttcggga tgctctccac caacgcgagt tttcccttat
    19021 ggtactcgtc aacaatccgc ttggccaaag tcaaccccaa cccccatccg cgctttttcg
    19081 tcgtgtagcc ggcgtgaaaa acttttttct gctcccggga gggaatcccc ttcccgttgt
    19141 ccgtgaccgt aatgcagact gttttttcgt cttttgacat gcggctggtc acctcaatgt
    19201 gccccgtgct agcgtccacc gcctccagag catttttgag aaggttttca atcacccatg
    19261 acaggagttc cggattggct tcgacggggg gcaattcctt caggttttcc ttgataacga
    19321 ccccctggcc gctgcccggc aagcgggtgc ggaagtagct ggcagcctcg tgaacgagcg
    19381 cattcagatc aaccggcttg agttcgggga gcgaaccgat ctgcccgaac cgctgggcga
    19441 ctctctggag ccggacaatg tcgccttcca tccgctccgc aatctccgcc agactgggat
    19501 tttcggcttg acccgtttct gccgtatggg atttgagaat ctcgagccac cccaaaagcg
    19561 aggtcaaggg cgtccccaac tggtgggccg tctccttggc catccccacc cagatgaaac
    19621 gctgctcgga ccgcttgatg ttccggtaac cgatgaaggc gacgaccaag aaaagtccaa
    19681 agagcgagag cagaatccag ggcatctgct gtagctgccg caccagtttc ggatcgccgt
    19741 agtgaaagta gtagatgatc cggtcgccgt agcggatcgg taacggcccg cgctggtggt
    19801 caaacttctc cagcatctgg tggacccggg ccaggttggc cggcgagcgg tctttctccg
    19861 gcacttgctc caggccccgc cagaactgga tgctcccccc tgagtcagtg atgataatcg
    19921 gaatgtttga cttttggatg atctcttcaa aaatgaacga aaccgccggt ccggtggtcg
    19981 gctcctgggt caccagctgc cacagccgcg cgtacatgtc cactactttg gcctggtctt
    20041 ccttcagttt cgcgatgacg ttctgggtgt accagatgaa aaacagagcg atcaccagaa
    20101 tgccgaagag caaaaagagg cggaagacca tcggcacccc ggtggcctgt ggccggttga
    20161 tctcggagtt ggtcacctac gtaatctccg ctaaaccgcc gaggtacggc tggagcacgt
    20221 cggggatccg caccgtccct ttttcggtct ggtaattttc caaaagggcc gcgagcgtcc
    20281 gcggcagggc cagtccggag ccgttgagcg tgtggggata gtcgggcttg gcgttcggcg
    20341 ccggccggaa gcgcacattc atccgccggg cctggaacgc ttcgtaattc gaacaggagg
    20401 aaacttccag aaaactctcg acgcccggcg cccagacttc gaggtcgtag cacttggccg
    20461 ctgcaaaaga gaggtcgccg gtggaaagga gacggacgcg gtacggcaga ccgagcaact
    20521 ggagaacctc ctcggcgtcg gtcactaatt tttccagttc agcatacgaa tgctccggac
    20581 ggacgatttt gaccaattcc accttatcaa actgatgaac ccggatgatg ccgcgcgttt
    20641 ccttgccgta ggagccggcc tcgcgccgga aacagggggt gtaggccgcg cggtagaccg
    20701 ggaggccatc ggcttccagt atttcgtcac ggaagagatt ggtcaccggc acctccgccg
    20761 tgggtaccag ccagaggtcg tcctgctcca ggcggtac
//
LOCUS       DBNGZA010000083         7895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2136460, whole genome shotgun sequence.
ACCESSION   DBNGZA010000083 DBNGZA010000000
VERSION     DBNGZA010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2136460"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="AC16FU_01820"
     CDS             complement(<1..726)
                     /locus_tag="AC16FU_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR2993148.1"
                     /translation="MTFVQTASPPRKFKELKDDFKALALEQEVLKFWREEKIFEKSIQ
                     SRTQAPPFVFYEGPPTANGKPGIHHVIARTIKDLICRYKTMQGFRVERKGGWDTHGLP
                     VELEVERELGLNSKARIEAYGVEKFNARCKESVFRYVEDWNALTERIGYWLDLRDAYI
                     TCDNNYIESVWWILAQFHQRGLIYKGHKVVPYCPRCGTGLSSHEVALGYEEITEDSIY
                     VRFRLKKDPKTSILSWTTTPRTVP"
     gene            complement(744..1589)
                     /locus_tag="AC16FU_01825"
     CDS             complement(744..1589)
                     /locus_tag="AC16FU_01825"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR2993149.1"
                     /translation="MQNKVSVNDLKNKINEAVATIRSRTKLAPQIGIVMGTGLGKLGE
                     NIKVETTIDYQDIPHFPLSTVESHAGRLIFGLLEGKPVVAMQGRFHYYEGYTMQQVTF
                     PVRVMKFLGAKVLVVSNACGGLNPGYKSGDLMVINDQINLQGDNPLIGPNDESIGPRF
                     PDMCGCYDRELVALAEKTALELGIKLHKGVYVSVTGPNLETAAEYRFLRIIGADVVGM
                     STVPEVIVARHMDLRVLGFSIITDMGLPDALKPTNLEEIIRVANASEPVLKKLMTETV
                     KRMKV"
     gene            complement(1670..2245)
                     /locus_tag="AC16FU_01830"
     CDS             complement(1670..2245)
                     /locus_tag="AC16FU_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR2993150.1"
                     /translation="MKTTPLDLRSQAFNKKMRGYDPDEVHTFLDAFADELEQHLKEHH
                     LLLEKFGLLESQVAEYRTMENSLRETLMAAQRASEEMRGHARKEADLVLQQAEVESQR
                     MVNETKDQLTQVRKELAELSMLKRNYMLQFRSLLDAHKLMLDDLEKSQAEGTQRTLRL
                     SRKGEVTDEEIEKVVQEFYRSSATRTEREVK"
     gene            complement(2299..3000)
                     /locus_tag="AC16FU_01835"
     CDS             complement(2299..3000)
                     /locus_tag="AC16FU_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR2993151.1"
                     /translation="MNTGPGPIRDNIRLLRERIFAICARLGRSPDEVTVVAVSKGHPA
                     RAIQEALLAGVTDIGENRVQEAEAKKREVAATARWHLVGHLQSNKARKAVALFDVIQS
                     VDTLPLAVKLAGLGAERGKPLRVLIEVKTSREATKFGVASEAVIDFAGKIRELFGLQL
                     EGLMTVGPLTDESDAIRHAFQTLKGLLERINAHLSPPERLAHLSMGMTADYEIALEEG
                     ATLLRIGTAIFGPRP"
     gene            complement(3096..3704)
                     /locus_tag="AC16FU_01840"
     CDS             complement(3096..3704)
                     /locus_tag="AC16FU_01840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/polyprenol kinase family protein"
                     /protein_id="HIR2993152.1"
                     /translation="MDERRPEAAAHEISFANEVKRKLIHLFAAVIPLGYLIVPRTLAV
                     VLLTVAVLVSLTLDVIRIYQLPPRRFVEKMLGSLLRHHESRDFTGGTYILSGALFSVI
                     LFPKPVAVAALGFTVVGDIAAALVGRRYGRVRFGAKSVEGSLACLAACFAVALVVPHL
                     SVWIGAVGALVATIVEATSLPVDDNLSMPLLSGMAMVGLLAF"
     gene            complement(3737..5506)
                     /locus_tag="AC16FU_01845"
     CDS             complement(3737..5506)
                     /locus_tag="AC16FU_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2993153.1"
                     /translation="MKVFASYLNPFFICLLWVGNQPGAQEAKPDPPAVTVDGAPIPFS
                     RFVEKAGALTPKPKTKVEIKESKEHIVNALIVEQIQEGLAAAIPVWDEPAFRQRVEKQ
                     LVQVALGELAGKLLTNAVTVSDSEVRAYFDTSTGQFDIPERVKASHILIAPMKDSSHV
                     DSLKKAQGLFLLTLEAAHGFAERLYGVVARGGAFDSLARLYSDDRLSGVKGGDLGYFA
                     YDEMEEPFAKAAFGMQPGEISRPVQTKYGYHIIKVWDHQPAGKVPLSDSVRTEITRTL
                     QTANVQIRSQAVIDSLRHAARVTFNDSVIAHSDSVRNSDLWVAIVNERDTVRFAEFWN
                     QLGWLRFGRRPEDSVNFAQKHRLVEQLLSAVLLKQAALITGCFEALSVQAARAQMIAE
                     EQKRRIEKQFQLDYEPSEQELIAYYDAHREEYKEAEPLHVQHIIFKDSSLARSVRDSI
                     VRLGLDFAAMAKKYYPGDPDIRGVAFDLGFISPQAMPKAFFDAASGLGIGEVSQPVKT
                     EWGYHLIKLVNRQTSKSFGAVRSDIAKLLIDRHLAQVRAKRAAEARKEHKIKVNKKLI
                     DAYQFAPEAPAAQAGAGQPAGSR"
     gene            complement(5522..6523)
                     /locus_tag="AC16FU_01850"
     CDS             complement(5522..6523)
                     /locus_tag="AC16FU_01850"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993154.1"
                     /translation="MKRLDWYPGKLQWGRWFWLTLFLGMVAASCPTSLFAAKFAGEFL
                     RLGVGARPLGMGSAFVAVADDITAGYWNPAGLAALSKREAMFMHAETFGSLLNHDYLA
                     FGLPVALGHGVGAASFIRLGGGGLKLTSLPDPTRPISLTNRPFVSRVASHGDYALFFS
                     YGLQRWPKIRLGASGKVLFRDLVDNSAFGLGIDLAAQAQVNSWVSAGITVQDATTSFL
                     AYDTGTKESITPTVKTGLAGRQMFGEITMTGAVDADFNFDNFRDAAQFWMGAISADTH
                     FGLEADYRGIGQVRIGSDIGKFSAGAGLRWRGWRLDVAYLNHPQLDNSYRISLAATW"
     gene            6700..7698
                     /locus_tag="AC16FU_01855"
     CDS             6700..7698
                     /locus_tag="AC16FU_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993155.1"
                     /translation="MNKTFPSAVETRKLRRLFKTKKKEGSGNGFLEALAGVDLTINRG
                     ELFGLLGPNGAGKTTLIKILSTLLLPTSGEALIDGTDVVAHPEQVRRKINVVSGGEHS
                     GYGILNVRENLWMFSQFYGVPTKLARERIDRMLALLGLTEFARTKLGKLSTGQRQKMN
                     LIRGFVPDPQILFLDEPTLGLDVQISREVRQFIREWVTNGQGKTVLLTTHYMAEADEL
                     CDRVAVIDRGRVLACDTPANLKKALRQEVVFKLEVSLLTDHLERLGQLEPVRNFAYEH
                     KPDQGRTILKLILHSDAGLPKITDFVTSNGSRILSISKMEPTLEDVFVAMVGRGLE"
     gene            7695..>7895
                     /locus_tag="AC16FU_01860"
     CDS             7695..>7895
                     /locus_tag="AC16FU_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993156.1"
                     /translation="MKTDLKTNLRAIWGRAYVRIVGANREPSWIIFETLMPLLATLTY
                     VFVYKALKAPEDYTGFVVLGGAM"
BASE COUNT         1619 a         2395 c         2216 g         1665 t
ORIGIN      
        1 cggcacagtc ctgggcgtcg tcgtccagga gagaatcgag gtttttgggt cctttttaag
       61 ccggaaacgg acgtagatcg aatcttcggt gatttcttca tatcccagag ccacttcgtg
      121 gctggaaagg ccggttccgc agcgcgggca gtaggggacg actttatggc ctttgtagat
      181 gagaccgcgc tggtgaaatt gggccagaat ccaccagacc gactctatat agttattgtc
      241 gcaggtgatg taggcatcgc gcaagtccag ccagtagccg atccgctcgg tcagggcatt
      301 ccagtcctcg acgtagcgga agaccgattc cttgcagcgg gcgttgaact tttcgacccc
      361 gtacgcttca atgcgggctt tggaattcaa gcccagttcg cgttccactt ccaactccac
      421 cggcaaaccg tgggtgtccc agcccccttt gcgctccacg cggaacccct gcatcgtctt
      481 gtagcggcag attaaatctt tgatcgtccg ggcgatgacg tggtggatgc ccggcttccc
      541 gttggcggtc ggcggccctt cgtagaagac gaaaggcggt gcctgcgtcc ggctctggat
      601 gctcttttca aaaattttct cctcccgcca gaacttgagc acttcctgct ccagcgccag
      661 ggccttgaag tcgtctttga gctctttaaa tttcctcgga ggtgaggcgg tttgcacaaa
      721 cgtcataaga aataattaca ctctcagact ttcattcgct tgaccgtttc ggtcatcaat
      781 ttcttcagca ccggctcgga agcgttggct acccggataa tttcctcgag gttcgtcggc
      841 ttgagcgcgt cgggcaagcc catatcggtg atgatggaga acccgagaac gcgcagatcc
      901 atatgccgcg ccacgatcac ctcgggaaca gtggacattc ccacgacatc agcaccgatg
      961 atccgtaaga accggtactc ggcggcggtc tccagattcg gcccggttac agatacgtaa
     1021 acgcccttgt gcagtttgat tcccaactcc agcgcggtct tctcggccag tgcgaccagt
     1081 tcccggtcgt agcagccgca catatcgggg aaacgcgggc cgatcgattc gtcattcggc
     1141 ccgatgagcg gattatcccc ctgcaggttg atctggtcat tgatgaccat aaggtcgccg
     1201 gacttgtagc ccggattgag cccgccgcag gcgttggaga caaccagcac cttggctccc
     1261 agaaatttca tcacccgcac cgggaaggtg acctgctgca tcgtgtaacc ttcgtagtag
     1321 tggaagcgtc cctgcatggc cacgaccggt ttgccttcca ggagcccgaa gatcagccgc
     1381 ccggcgtggc tttccaccgt ggagagcggg aaatgcggaa tgtcctgata gtcgatggtc
     1441 gtttcgacct tgatgttttc gcccagcttc cccagcccgg tgcccatgac aatgccgatc
     1501 tgtggcgcca gtttcgtccg gctgcggatg gtcgctaccg cttcgtttat tttattcttg
     1561 agatcattca ccgatacctt gttctgcata gaagtccttg tctgtgttga tatccgtagg
     1621 tagtccggct cgtcccggag ccggacacct tgcgttttca cctaaccacc tacttcactt
     1681 cccgctcggt acgagtcgca ctcgaccggt agaattcctg taccaccttt tcgatttctt
     1741 cgtcagtgac ctcgccttta cgcgacaggc gcagggtgcg ctgggtccct tcggcttgcg
     1801 atttttccag gtcatccagc atcaatttgt gcgcgtccag aagcgagcgg aactgcagca
     1861 tgtagttgcg cttgagcatg gagagctcgg ctaattcttt ccgcacctgg gtcaactggt
     1921 ctttggtctc gttcaccatc cgctgcgatt cgacctccgc ctgctggagg accaggtcgg
     1981 cctccttcct ggcgtggccg cgcatctctt cggaggcgcg ctgggccgcc atcagggtct
     2041 cccgcaggga attttccatc gtccggtact ccgccacctg ggactccaaa agcccgaatt
     2101 tctccagaag caggtggtgc tccttcaagt gctgttccag ttcgtcggca aaagcatcca
     2161 gaaaggtgtg gacctcgtcc gggtcatacc cgcgcatttt tttgttgaag gcctggctgc
     2221 gcagatccaa aggcgtggtt ttcatcgtcg gctccttttc ctgtcctcaa ccggaaccac
     2281 cggacaacct tcccaccgtc acgggcgcgg cccgaagatc gccgtcccga tccgtaagag
     2341 cgtggcgcct tcttcgaggg cgatctcgta gtcggcggtc atccccatcg acaggtgcgc
     2401 cagccgctcc gggggactca ggtgtgcgtt gatccgttcc aaaagaccct tgagcgtttg
     2461 aaatgcgtgg cggattgcgt cgctttcatc cgtcagcggg ccgacggtca tcagtccttc
     2521 caattgcagg ccgaaaagtt cacggatctt tccggcgaaa tcgatcaccg cttcggatgc
     2581 cacgccgaac ttggtggcct cgcgcgacgt cttcacctca atgagtaccc gcaggggctt
     2641 cccgcgctcg gcgcccagcc cggctaactt taccgctaat ggcagcgtgt ctaccgactg
     2701 aatgacgtca aaaagtgcga ccgccttccg cgccttattg ctctgcagat gtccgaccaa
     2761 gtgccagcgc gcagttgccg cgacctcccg ctttttcgct tccgcctcct gcacccgatt
     2821 ctcgccgata tcggttacgc cggctaaaag cgcttcctga attgcccggg ccgggtggcc
     2881 tttgctcacg gccactaccg ttacctcatc cggactacga cccaaccgcg cgcagatagc
     2941 aaaaatccgc tcccgcagga gccggatatt gtcgcgaatc ggccccggac cagtgttcat
     3001 ccctcaccaa cctggcccat gcggagggag atattacacc gggaaaggcc aaaatgcaac
     3061 agccgatgcg gcccgccttt ctgcagggag aggatttaaa aggccaaaag accgaccatc
     3121 gccattccag acaaaagcgg catcgagaga ttatcatcca ccggcaggct ggtcgcctcc
     3181 acaatggttg ccacgagagc accgacggca ccgatccaga ccgagaggtg gggaacgacc
     3241 agggcaacgg cgaaacaggc cgccaggcac gccagactgc cttccaccga tttcgccccg
     3301 aaccgcaccc gcccgtaccg gcgacccacc agagccgccg caatatctcc cacgacggtg
     3361 aacccgaggg cggcaaccgc caccggcttg ggaaaaagga tgacagagaa aagcgccccg
     3421 gacaatatgt acgtgccacc ggtaaagtcg cggctctcat ggtggcgcaa aagcgatccc
     3481 agcatctttt cgacgaaacg tcgcggcggc agttggtaga tgcgaatgac gtccaacgtg
     3541 agcgaaacca aaacggcaac agttaaaagg acgacggcga gcgtacgtgg tacgatgagg
     3601 tagcccaggg gaatgacggc cgcaaacagg tgaatcagtt tgcgcttgac ttcattggca
     3661 aaggatatct cgtgagcggc tgcttccggt ctccgttcgt ccacggtggg aggcagaagt
     3721 gtgcttcggc ggtgacctac cgggagccgg cgggctgacc cgcgccagct tgggctgctg
     3781 gcgcttccgg cgcaaattgg taggcgtcga tcagtttctt attcaccttg atcttgtgct
     3841 ctttgcgcgc ctccgccgcc cgcttggccc ggacttgcgc caggtgccgg tcgatgagaa
     3901 gtttggcaat gtcagaccgt actgcgccga agcttttgct cgtctgccgg ttcaccaatt
     3961 tgatcaggtg gtacccccat tccgttttga ctggttggct cacttcgcca atccccagcc
     4021 ccgaggcggc atcgaagaac gcttttggca ttgcctgcgg ggaaatgaag cccaagtcga
     4081 aagccactcc tcggatgtcg ggatcgccgg gataatactt cttggccatc gcggcaaaat
     4141 ccaagcccag acggacgatc gagtcgcgca ccgatcgggc cagggacgag tccttgaaaa
     4201 taatgtgctg gacatggagc ggctcggcct ctttgtactc ttcccggtgg gcatcatagt
     4261 aggcaatcag ttcctgttcc gagggctcgt agtcgagctg gaactgcttt tcaatccgcc
     4321 gtttctgttc ctcagcgatc atctgggccc gggcggcttg gacgctgagc gcctcgaagc
     4381 acccggtgat cagtgccgcc tgcttcagga gcaccgccga aagtaactgc tccaccagcc
     4441 ggtgcttctg ggcgaaattg acgctgtcct ccgggcgtcg cccgaagcgt aaccagccga
     4501 gctgattcca gaactctgca aaacgcacgg tgtcccgttc attgacgata gccacccaga
     4561 gatcggagtt ccggaccgaa tcactgtggg cgatgaccga atcgttgaac gtcacccggg
     4621 cagcgtggcg caagctgtcg attactgctt gtgaacggat ttgaacattg gcggtttgta
     4681 acgtccgggt gatctcagtg cgtacggagt cgctcaaggg caccttgccc gccggctggt
     4741 gatcccacac tttgatgatg tggtagccgt acttcgtttg gaccggccgg gagatctccc
     4801 cgggctgcat cccgaaggcc gctttcgcaa acggctcttc catctcgtca taagcgaagt
     4861 agccgaggtc gcctcctttg accccgctca gccgatcgtc cgagtacaaa cgcgccaggg
     4921 aatcaaaggc gcctccccgg gcaaccaccc catacaagcg ttcagcgaag ccgtgtgccg
     4981 cctcaagggt gaggaggaac aagccctggg cctttttgag tgaatcgacg tgactggaat
     5041 ccttcatcgg ggcaatcagg atgtggctgg ccttcacccg ttcggggata tcgaactggc
     5101 cggtactggt atcgaagtag gctcgcacct cgctgtcgga aacggtgacg gcgttcgtga
     5161 gcaacttccc ggctagttct cctaacgcga cctggactaa ctgtttttcc actcgctggc
     5221 gaaatgccgg ttcatcccag accggaatag ccgccgctaa accctcttga atttgctcga
     5281 caatcagtgc attgacgatg tgctctttac tctctttaat ttcgactttg gtttttggct
     5341 tgggcgtaag cgccccggct ttctccacaa agcgggagaa ggggatcggc gcgccgtcga
     5401 cggtgacggc cggtggatcc ggcttggctt cttgtgcacc cggctgattg cctacccata
     5461 agaggcagat aaaaaatgga ttcaagtacg acgcgaaaac tttcattgca tttccctcat
     5521 ttcaccaggt ggctgccaga gaaatcctat aagagttatc taactgcgga tggttgaggt
     5581 acgcgacatc cagccgccac ccgcgccagc gcaggccggc gcccgcgctg aacttgccaa
     5641 tgtctgagcc aatccgaacc tgcccgatcc cgcgatagtc ggcttccaac ccgaagtgcg
     5701 tgtccgccga gattgcgccc atccagaact gcgcggcgtc ccggaagtta tcaaagttga
     5761 agtcggcgtc aacggcaccg gtcatcgtta tttctccgaa catctgccga ccggcaagcc
     5821 cggtcttcac cgtcggcgtg atgctttcct tggtgccggt atcgtaggcg agaaaggaag
     5881 tagtagcatc ctgaaccgtt atccccgcgc tcacccagct gttcacctgc gcttgggcgg
     5941 ccagatcgat ccccaaaccg aaagcggagt tatcgaccag atcgcggaag agcactttgc
     6001 ccgaagcgcc gagccgaatt ttgggccagc gttgcagtcc gtaggagaag aaaagagcat
     6061 agtcgccgtg ggaagccacc cgggagacaa acggccggtt ggtgagagaa atcggacgcg
     6121 tgggatcggg cagggaggta agtttcaacc cccctccgcc cagacggatg aaactggccg
     6181 ccccgacgcc atggccgagt gccaccggca gcccgaaggc caggtaatca tggttcagaa
     6241 gcgaaccaaa cgtctccgca tgcatgaaca tcgcctcgcg cttcgacagg gcggccaatc
     6301 cggccgggtt ccagtaaccg gcggtgatgt catcggccac cgccacaaag gcgctgccca
     6361 tgcccaaagg ccgcgctccg acccccaggc gcaggaactc cccggcaaat ttggccgcga
     6421 aaagtgaggt cggacaggac gctgccacca tgcccaaaaa aagcgtcagc cagaaccagc
     6481 ggccccattg cagttttccc ggataccagt ccaatcgttt catcccaacc tgcctaaaat
     6541 taggcggtca gaccctaact gtcaatttta tacggaggaa tctccgccgc cgtgcttgac
     6601 aaatcctctt tcggcgcata tccagaggag gcgtctgtgg cgatttgact acttggcttc
     6661 gtacccagcc cggcattttc gttctgttgg agcaacgcaa tgaataagac atttccgtcc
     6721 gccgtcgaaa ccagaaaact gcggcggctg ttcaaaacca aaaagaaaga gggttccggt
     6781 aatggtttct tagaagccct ggcgggtgtg gacttgacca tcaaccgggg ggaactcttc
     6841 ggcctcttgg gccccaacgg cgccgggaag accacactca taaagatttt atcgacgtta
     6901 ttgctcccca cctccggtga agctttgatc gacggcaccg acgtggtggc ccacccggaa
     6961 caggtgcggc ggaagatcaa cgtcgtctcc ggcggggaac actccggcta cggcattctg
     7021 aacgtccgcg aaaacctctg gatgttctcc cagttctacg gcgtgccgac caagctcgcc
     7081 cgcgaacgga tcgaccggat gctcgcactt ttgggactga ccgagttcgc ccgaaccaaa
     7141 ctggggaagc tctccaccgg acaaaggcag aagatgaatt tgatccgcgg tttcgtcccc
     7201 gacccgcaga ttctctttct ggacgaaccg acgttggggc ttgacgtgca gatctcccgc
     7261 gaagtgcgcc agttcattcg cgagtgggtc accaatggtc aaggaaagac ggtgctcttg
     7321 accactcact atatggccga agccgatgaa ctgtgcgacc gcgtagccgt gatcgaccgc
     7381 ggcagagtcc tggcctgcga tacaccggcg aatttaaaga aagcgctccg gcaagaagtg
     7441 gtgttcaaac tcgaagtgtc gcttttgacc gaccacctgg agcgcctggg ccaactggaa
     7501 ccggtacgga atttcgccta tgagcataag ccggatcaag gccggacgat tttgaaatta
     7561 atcctgcaca gcgacgcggg gctgccgaaa ataactgact tcgtcacgag taacggctcg
     7621 cgcattctct cgatttcgaa aatggaaccg accttagaag acgttttcgt ggcgatggtc
     7681 ggcagaggtt tagagtgaaa acagatctga aaaccaacct ccgcgccatc tggggccgcg
     7741 cctacgtgcg gatcgtcggg gccaaccgcg aaccctcctg gattatcttt gagaccttga
     7801 tgccgcttct ggccaccctc acctacgtct tcgtttacaa ggccctcaaa gcgccggaag
     7861 actacaccgg ctttgtagtt ttaggcgggg cgatg
//
LOCUS       DBNGZA010000084         9739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2136540, whole genome shotgun sequence.
ACCESSION   DBNGZA010000084 DBNGZA010000000
VERSION     DBNGZA010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2136540"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1701)
                     /gene="groL"
                     /locus_tag="AC16FU_01865"
     CDS             complement(73..1701)
                     /gene="groL"
                     /locus_tag="AC16FU_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR2993157.1"
                     /translation="MPKLIEYNTTAREKLKRGVDKLADAVKVTLGPRGRNVVIDKKFG
                     SPTVTKDGVTVAKEIELEDHFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFREG
                     LRNVTAGANPMDLKRGIEKAVGVVVGELKKISKPVPGKTEISQVGAISANNDKVIGDL
                     IADAMEKVGKDGVITVEEAKTTETKLEVVEGMQFDRGYLSPYFITNPDTMEAVLEEAL
                     ILIHDKKISTMKDLLPILEKVAQVGKPLLIVSEDVEGEALATLVVNKLRGTLRVCGVK
                     APGFGDRRKEMLQDIAVLTGGKVISEELGFKLENTVMSDLGTAKRITIDKDNTTIVEG
                     GGKSSEIKGRVNSIKKQIEETTSDYDKEKLQERLAKLAGGVAVINVGAATETEMKEKK
                     ARVEDALHATRAAVEEGIIPGGGVAYLRCLAALDKIKENGDVAIGVNIVRKALEEPIR
                     MIAGNAGVEGSLVVNRVKEEKGAFGFNAENEKYEDLYTAGVVDPTKVARIALENAASI
                     AGLLLTTEAIIAEKPEEKKAPAMPPGGGYGGDMY"
     gene            complement(1831..2130)
                     /gene="groES"
                     /locus_tag="AC16FU_01870"
     CDS             complement(1831..2130)
                     /gene="groES"
                     /locus_tag="AC16FU_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR2993158.1"
                     /translation="MQVKPLADRVLIKPLETQETKKGGIIIPDTAKEKPQQGEITAVG
                     PGRITEDGKKIAMEVRKGDRVLYGKYSGTEVTIDDLECLIMRESDILAVIENGKK"
     gene            2532..3242
                     /locus_tag="AC16FU_01875"
     CDS             2532..3242
                     /locus_tag="AC16FU_01875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2993159.1"
                     /translation="MAKESIKDRAYYDQAGPYFERLKREYLQDTRWKRNRVRNVLNLA
                     RPQPGDVVLDLGCGIGTFVFESARRNARAYGIDFSITALSTAREIAAGQGLRVGGFVA
                     GDAGALPFQSESFTLIICADLVEHLYENTYRSMLAECHRTLTRGGRLAIYTPSPTHLF
                     ERLQHHEILLQKDESHIGLKTMPYLLETLRQAGFVIPHYYFRPTHLPVYRWVESCFIP
                     IPGLGRPFRRRSCILAMK"
     gene            3333..5798
                     /locus_tag="AC16FU_01880"
     CDS             3333..5798
                     /locus_tag="AC16FU_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993160.1"
                     /translation="MAKKKVHAKGCGRLQPAAPGGIVLAPPLQRLLPVVAYAIFTLLL
                     FREFIFSKGMLYGADTFQAGVFFRNFFVQFVKTFHELPQWDPYLFCGMPYLDAFHGDI
                     LYPLSLPFKFLLPLSRALGWILVFHIFLAGCFMYLCARGFGLGKFASFFAGTVFMLSS
                     YLVSLVHPGHDGKIFITALFLLALFFLHRGLEKKRAAFFVYFGVVVGVIIITPHPQMA
                     YFSLWGLGAYTVFRLVIHLRTEKKILETARLGVFTALAVLLALAISAIQFWPGYVYVK
                     QYSPRAEGAKDFAWATSWSLHPEELVSQVVPEFSGQHLGTGKNDYWGRNPFKDNSEYA
                     GLIPLLLAGIALVGARRKEAWFFCGLGLFVLIYALGATTPFFHFFYAVIPNIRNMRAP
                     SMIMFLFAFCSSLLGAMGLEEVFRRIKQGKLGESQKVVHYLWGATALLGLGAIIVTFS
                     GQGFAGLWKSIFWTTMPPERLVQLRLNLPRITLGFWLATFFVGICAFAIQRGLKGVWK
                     PTLVGAIIWTVAFFDLWRMDGRFIYVVDPQRVFGLHPSVEFLKQDPEPFRCFMQNDNF
                     SRDYFPYYGIELVSGYHGNQLRWYQEFLEAAQSETLGGFRFWNLINTKYFILPHGVDI
                     PRDPALGLDKVYDEQNVTIYRNLNAQPRAFVVHQIEVIPDREKILPRLADPTFDYRRK
                     IILEEDSKVALPPADTLATAEPVRISDNRINSFTVTATLDRPGFLFLSDTYYPAWKVL
                     EGNRERTIYRADYAFRAVYLEAGTHVLRFVYDSALYRIAKQVTIFSTLFAFAFLGGDL
                     AWRRWKKKRGKIAIPPVSSDTGG"
     gene            5901..6251
                     /gene="hpf"
                     /locus_tag="AC16FU_01885"
     CDS             5901..6251
                     /gene="hpf"
                     /locus_tag="AC16FU_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR2993161.1"
                     /translation="METRVTARHFELSDPLRRLAEEHSARLRRFFDRIIENHWTLTRE
                     KSRQTAELTVKVYGTVLTSKVVSHDIVLAVEGAADKMERQLKKYKERLKEKDPKRIST
                     LKTGGEIRPGAAEY"
     gene            6262..7230
                     /gene="hprK"
                     /locus_tag="AC16FU_01890"
     CDS             6262..7230
                     /gene="hprK"
                     /locus_tag="AC16FU_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR2993162.1"
                     /translation="MELISVEQLYRDKKEYLELSVLTGNSGLSKLVLNNEVHRPGLAM
                     AGFLDRFASQRTQVLGETELAYLVSFDEKQRAEIAERIFGQNLPVVFVTKGLAPPADF
                     LEVAVRHETAVLSSRLTTTEFVSRISTYLDHLFAPQTTIHGTLVDVYGVGLLYTGKSG
                     IGKSECALDLVERGHRLVADDMVRIIRKTPEVIIGTGSELLGHHMEVRGIGIIDIEKL
                     FGIRAIRMQKRIEVEVRLVAWDEEAEYERLGVEDKFTTILGVEIPVVTIPLSPGKNIT
                     VISEVIAMNHMLKIYGEHTAQEFSKRLSEEIHRRKMTTTYLESDYE"
     gene            7467..8363
                     /locus_tag="AC16FU_01895"
     CDS             7467..8363
                     /locus_tag="AC16FU_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR2993163.1"
                     /translation="MNNRTTELRVGLAVLIGLVILIWGLIWLKGFRFQRQRYEVNVLF
                     PNIGTLKTGDMAAVSGVDKGKVEKIQLQRGDVMVKMSLDKDVVLKADARFTVMNVGLM
                     GERFVEVRTGYSDTLLNLREPIYGFYDTGIPEVMGMLGRGIDELRELIAALRGTVASP
                     GYLSKVQDVIANTQKLTERLNAVVEGNQRKIGQATEDFASAAGRLKTLLETNAPKIDS
                     TVTSAREAASRMNEITTGLDSLSHSLHSFIDKIDSQQGTLGKLVNDDRLYGEIRKAAN
                     DLDSLVLDIKKNPKKYLRIQIF"
     gene            8538..>9739
                     /locus_tag="AC16FU_01900"
     CDS             8538..>9739
                     /locus_tag="AC16FU_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2993164.1"
                     /translation="MKRETNPIRISVVIPALNEVQLLPHCLRSLKNQNFGHSYEIIVV
                     DNNSEDRTAAVAAGMGARVVFEPHRGITWARQKGLEAARGEIIAWVDADTVVTVDWLQ
                     QIYAALNDADGVVAVSGKILYTKSTHWKGKLPGYWVAAVRYGDRAIRFLLRKPGTLWG
                     GNFAVQKKALLQVGGFNKNIEFYGEDTELSLRLGKAGKIQFNKNQIAYTSPRRFENQP
                     LIRTAWFYVSNFVRIMVFHRYAASRPKPGFPALKIYFQKVLPVFGMLSILVAFGFFAF
                     NPASQFYGRVYSAGLDWRQKVIALSFDDGPDEPYTSQVLNILDENGIKATFFIIGKNA
                     EYYPEAVREIVDRGHIIGNHSYSHYYLLPFENNDRIQKDLIKTENLLFHLTGLRTALF
                     RPPHGLRTP"
BASE COUNT         1981 a         2642 c         2711 g         2405 t
ORIGIN      
        1 ggggctctta gccccgccag cccgtcaacc aaaagccccg cctctggcgg ggcttttggt
       61 tctttctgta gattagtaca tatctccgcc atagccaccg cctggcggca tggccggagc
      121 cttcttctcc tcgggtttct cggctatgat tgcctcggtg gtcaagagca acccggcgat
      181 cgaggcggcg ttctccagcg caatccgcgc caccttggtc gggtccacga cacccgcggt
      241 gtagaggtct tcgtatttct cgttttcggc gttgaagccg aaggcgcctt tctcctcctt
      301 cacccggttg accacgaggg agccctccac cccggcgttc ccggcgatca tccggatcgg
      361 ttcctccaac gccttgcgga cgatgttgac cccgatagcg acgtcgccgt tttctttaat
      421 tttatctaag gccgccaagc agcgcaggta ggccaccccg ccgccgggga taatcccttc
      481 ctcgaccgcc gcgcgcgtgg cgtggagcgc gtcttcgacg cgggccttct tctctttcat
      541 ttctgtctcg gtggcagcac cgacgttgat caccgccact ccgccggcca acttcgccag
      601 gcgctcctgc aacttctcct tgtcatagtc ggaggtggtc tcttcgatct gttttttgat
      661 cgaattcacc cggcccttga tttccgacga tttgccgccg ccctcgacga tggtggtgtt
      721 gtccttgtca atggtgatgc gcttggcggt gcccaggtcc gacatcaccg tgttctccag
      781 tttgaaaccc agttcctcgg agatcacctt gccgccggtc agaaccgcga tgtcctggag
      841 catctccttg cggcggtcgc cgaaaccggg agctttgact ccgcaaaccc gcagggtgcc
      901 gcgcaatttg ttgacgacga gagtcgccaa tgcctcgccc tccacatctt ccgagacgat
      961 caagagcggc ttgccaacct gcgccacttt ttccaggatc ggcagaagat ctttcatcgt
     1021 ggagattttc ttgtcgtgga tgagaatgag cgcttcctcc agcaccgctt ccatcgtgtc
     1081 gggattggtg atgaagtagg gcgagagata gccgcggtcg aactgcattc cctccaccac
     1141 ttcgagtttg gtctcggtgg ttttggcttc ctcgactgtg atgacgccgt ctttgcccac
     1201 cttttccatg gcgtcggcaa tcaggtcgcc gatcaccttg tcattattgg cggaaatggc
     1261 gccgacctgg gagatttcgg tcttccccgg caccggcttg gagatttttt tcaattctcc
     1321 gaccaccacg ccgaccgctt tttcaatccc gcgcttcaaa tccatcgggt tggcaccggc
     1381 ggtgacgttc ctcaaacctt cccggaaaat cgcctgagcc aggacggtag cggtggtagt
     1441 gccgtcaccg gccacatcag aggttttgga ggcaacctct ttgaccatct gggcgcccat
     1501 gttctcgaag tggtcttcga gttcgatttc cttggccacg gtgacgccgt ctttggtgac
     1561 ggtgggggag ccgaacttct tgtcaattac tacgttccgg ccgcgcggac ccaaagtcac
     1621 ttttaccgcg tcggccagtt tgtccacgcc cctttttaat ttctcccggg cggtggtgtt
     1681 gtattcaatt aacttcggca tacactaaac ctccgttttg tttttgttta gggtgttttg
     1741 gagtgtcgaa gtttcgaagt gtcgaggttg aaagactttg tccttaactc tgacactccg
     1801 acactacgat acttcgacac gcagtttcct tcacttcttc ccgttctcga tcacggcgag
     1861 gatgtcggat tcgcgcatga tcaggcattc caaatcatcg atggtcacct cggtacccga
     1921 gtatttgccg tagagcacac ggtcgccttt tctgacttcc atcgcgatct tcttgccgtc
     1981 ctcggtgatc cgccccggcc caacagcggt gatctccccc tgctggggtt tttccttggc
     2041 tgtgtccggg atgatgatcc cgcccttttt ggtttcctgc gtctctaagg gcttgatcag
     2101 tacccggtct gccagcggtt ttacctgcat tgcttttccc tcctttgggg ttttattaaa
     2161 ttttccctga gtgtgagtga aatcgattta cacctcgcac ttttagcact cattcgaaga
     2221 gagtgctaac aaaattaaaa cctccaatga atattgtcaa gcgaaaaaaa tgtcggaagg
     2281 gcatttttta tttaactcat tgtctgacag attgttatag tggaattatg tgggcttagt
     2341 actttttaga gcaatcttaa aggagtgaac tcaagtccgt tctggttctt tttttttggg
     2401 gtcggtagga gtaatagccc tgcgaggccg caggtcttcg ccaactatcg tttaccattt
     2461 gaattgaata agactccttg ccaaataatt gggttctgtg tgtattgatc aatttggaga
     2521 cgatagggag aatggcaaag gaatcgatta aagatcgcgc ttattacgat caagccggtc
     2581 cgtatttcga gcgcctgaag cgggagtacc tccaagacac gcgctggaag cgcaaccgcg
     2641 tccgtaacgt cttaaactta gcccgaccgc aaccgggcga cgtggtgttg gatttgggct
     2701 gcggcatcgg gactttcgtt tttgaaagcg cccggaggaa tgcccgggcc tatggtatcg
     2761 atttttcgat aaccgctctt tccacagcgc gcgaaattgc tgccggtcag ggcttgcggg
     2821 tgggcggctt tgtcgccggg gacgccggag cactcccgtt ccagagcgaa agttttacgc
     2881 tcatcatctg tgccgatctg gtggaacacc tctacgaaaa tacctatcgg agtatgttgg
     2941 ccgaatgcca ccgcaccttg actcgcggtg gtcggctggc catttacacc ccgtcaccga
     3001 cgcacctgtt cgaacggctg cagcaccacg aaatcttgtt gcaaaaagac gaatcccaca
     3061 tcggcctcaa aacgatgccg tatctcctgg aaactctccg gcaggccggg tttgtcatcc
     3121 cgcactacta ctttcgcccc acacatctgc cggtttaccg ctgggtggag tcttgtttca
     3181 taccgatacc gggtctgggg cggcccttcc ggaggcggag ttgcattctg gcgatgaagt
     3241 aggtcgtccc ccccttcgtg tttttaaaaa cgaaactggc cgtctgcgag ttttggcttg
     3301 acaggaagga cttctttctg cctatttaac ctgtggcgaa aaagaaagtc cacgcgaaag
     3361 gctgtggacg actgcaaccc gccgcacccg gcggtatcgt tcttgctcct cccctgcagc
     3421 gtctgttacc ggtggttgcc tacgctattt tcacgctgct tttgttccgc gagttcatct
     3481 tctccaaggg catgctttac ggggccgaca cgttccaggc cggggtcttc ttccgtaatt
     3541 tctttgttca gttcgtcaaa actttccacg aattgccgca gtgggatccc tatcttttct
     3601 gtggcatgcc gtacttggat gccttccacg gcgatattct ctacccgctc tcactcccct
     3661 tcaaattcct tttgccgctc tcccgggctc tgggctggat tctggtcttt cacatcttcc
     3721 tggccggttg cttcatgtac ctctgtgcgc gcggcttcgg tctggggaag tttgccagtt
     3781 ttttcgccgg aacggtcttc atgctctcca gttatctggt ttcgttggtc catcccggac
     3841 acgacgggaa gattttcatc accgccctgt tccttttggc attgtttttc ctccaccggg
     3901 gattagaaaa gaaacgagcg gcattcttcg tttatttcgg ggttgtggtc ggggtcatca
     3961 tcatcacgcc gcacccccag atggcgtatt tttccctctg ggggctgggc gcctacacgg
     4021 tcttccgcct ggtcatccac ctgcgcacgg agaaaaaaat tttggagacg gcccggttgg
     4081 gggtgtttac cgcactggcc gttcttttgg cactggcgat ttcggcgatc cagttctggc
     4141 cggggtatgt ctacgtgaaa cagtactccc cgcgggcgga gggagccaaa gacttcgcct
     4201 gggctacttc ctggtcgctc catcctgagg aattagtgag tcaggtcgtg cccgaattct
     4261 cgggccagca cctgggaacg gggaaaaacg actactgggg gcgcaatccg ttcaaggata
     4321 actcggagta cgccggcttg atacctttac ttttggccgg catcgctctg gtcggcgccc
     4381 ggcgcaaaga agcctggttt ttctgcgggt tgggtctttt tgtcctgatc tacgctttgg
     4441 gagccacgac cccctttttc catttctttt acgccgtcat ccccaacatc cgcaacatgc
     4501 gggcgccgtc gatgattatg ttcctgtttg cgttctgctc gtcgcttctg ggagcgatgg
     4561 gattggagga ggtgtttcgg cggataaagc aagggaagct gggcgaatcc caaaaggtag
     4621 tccactacct gtggggggcg acagcgcttc tggggctggg tgcaattatc gtcaccttct
     4681 ccgggcaggg attcgcgggg ctgtggaagt cgattttctg gacgacgatg cccccggagc
     4741 gcctcgtgca gttgcggttg aatttacccc gcattaccct gggattctgg ctggccactt
     4801 tttttgtcgg tatctgcgct tttgccattc agcgcggatt gaaaggggtc tggaaaccaa
     4861 ccttggtcgg tgcgatcatc tggaccgtgg ctttttttga cctctggcgg atggacggcc
     4921 gcttcatcta cgtggtcgat ccccagcggg ttttcggatt gcacccgtcg gtggaattcc
     4981 tcaaacagga tccggaaccc tttcgctgct ttatgcaaaa cgacaacttt tcccgtgatt
     5041 atttccccta ttacggcatc gaattggttt ccggctacca cggtaaccag ctccgctggt
     5101 atcaagagtt cctggaagcg gcgcagtcag agactctggg tggtttccgg ttctggaacc
     5161 tcatcaacac caagtacttt attttaccgc acggggttga tattccgcgc gatccggctc
     5221 tggggttgga caaggtctat gacgaacaga atgtgaccat ctaccgcaac ctgaacgctc
     5281 agccccgggc ttttgtggta caccagatcg aggtcattcc cgaccgggag aaaattttgc
     5341 cgcgcttggc cgatccgacc ttcgattacc gacgaaaaat cattctcgaa gaagactcaa
     5401 aggtcgccct cccgccggcc gatactctgg caacggctga gccggtacgg attagcgata
     5461 accgcatcaa ttctttcaca gtgacggcga ctctggacag accgggtttt ctctttctga
     5521 gcgacaccta ctatccggcc tggaaagttc tggaagggaa ccgggagcgc accatctacc
     5581 gggcggatta cgccttccgc gcggtctatc tcgaagcggg gacgcatgtc ctccgctttg
     5641 tgtacgattc cgccttgtac cggatcgcca agcaggtcac gatcttttcc acgctttttg
     5701 cttttgcctt tctgggcggg gatctggcct ggcggcgctg gaagaaaaaa aggggaaaaa
     5761 tcgcaatacc tcccgtctct tctgacacgg ggggatagaa aatgcgtata ttaattcagg
     5821 gaataagatg cgggtacgat tcgcgagaga tgtccacctc ttaacccatc acctttcaat
     5881 gacttaagaa aggagcggtt atggaaaccc gtgttaccgc gcgccatttt gagctatctg
     5941 atcctttacg gcgattagcc gaagagcatt ctgcccgcct gaggcggttc tttgaccgga
     6001 tcatagaaaa ccactggacc ttaacccggg aaaagtcgcg ccagaccgca gagttaaccg
     6061 tgaaagtgta cggcacggtg ctgacgagca aagtcgtttc gcacgatata gtgctggcgg
     6121 tggaaggggc cgccgataag atggaaaggc agttgaaaaa atataaagag cggctgaaag
     6181 aaaaggaccc aaagagaata tccaccctga aaacgggtgg agagattcgt cctggagcag
     6241 ctgaatattg atgggcgaag catggaactg atttcagtcg aacagctcta ccgcgataaa
     6301 aaagagtatc tggaactttc cgttttgacc ggcaactcgg gcctctccaa gctcgttttg
     6361 aacaatgagg tgcaccggcc ggggctggcg atggccgggt ttctggaccg ctttgccagc
     6421 cagcggacgc aggttctggg cgaaaccgaa ctggcctacc ttgtcagttt cgatgaaaag
     6481 caacgcgcgg agatcgccga gcggattttc ggacaaaatc tgccggtggt cttcgtcacc
     6541 aaagggctgg cgccgcccgc cgatttcctg gaggtggcgg tgcgtcacga aaccgcagta
     6601 ttaagctcgc gtttgacgac cactgaattc gtttcccgga tttcgaccta cctggatcac
     6661 ctctttgcgc cgcagaccac catccacggc accttagttg acgtctacgg ggtggggctg
     6721 ctctacaccg gcaaaagcgg catcggcaaa agcgagtgcg ccctcgatct ggtggagcgg
     6781 ggccatcggc tcgtcgccga cgacatggtg cggataatcc ggaagacgcc ggaagtgatc
     6841 atcggcaccg gctccgaact tttgggccac cacatggagg tgcgcggcat cgggatcatc
     6901 gacatcgaga aattattcgg catccgcgcc attcggatgc agaagcgaat cgaagtcgaa
     6961 gtgcgtttag ttgcctggga cgaagaagcg gagtacgagc ggctgggggt cgaagacaag
     7021 tttaccacga ttctgggagt ggagattccg gtggtgacga ttccgctttc gccgggcaag
     7081 aacatcacgg tgatctccga ggtaatcgcc atgaaccaca tgctcaaaat ctacggcgag
     7141 cacacggccc aggagttctc caaacggctt tccgaagaga tacaccggcg caagatgacc
     7201 accacctact tggaaagcga ctacgagtaa gcaggtagca ggtggtgggt gagaggtggt
     7261 agggggaaaa agttcatctc tgggggctga ttgtattcag cccctccatt ttgagcacat
     7321 cccttacggt tacttggttt gtatgaggtc gccccgacat gcgggggctc ggggtatgcc
     7381 ggaggcgatt ttaggccgat gacaaaaaga gggaatctat ccggagtttt ggagggacgg
     7441 caagagtttt gtaggttgtc ggcgcgatga ataaccgcac caccgaactg cgcgtggggc
     7501 tggccgtttt gatcggcctg gtcatcctca tttggggttt gatctggctc aaggggtttc
     7561 gcttccagcg ccagcggtac gaagtcaacg tccttttccc caacatcggg acgctcaaga
     7621 ccggggatat ggcggcggtc tccggcgtgg acaaagggaa ggtcgaaaaa atccagctcc
     7681 agcggggcga cgtaatggtc aagatgtcct tagacaagga cgtggtgctc aaggccgatg
     7741 cgcgctttac ggtgatgaac gtgggcttga tgggggagcg cttcgtggaa gtccgcaccg
     7801 gctactccga tacgctcctg aacctccggg agccgattta cggcttttac gacacgggga
     7861 tacccgaagt gatggggatg ctgggccggg ggattgacga actgcgggag ttgattgcgg
     7921 ccctgagggg gacggtagcc tcgcccgggt atctctccaa ggtgcaggac gtgattgcca
     7981 atacgcagaa gttgaccgag cggttgaatg ctgtggtgga gggcaatcaa aggaaaatag
     8041 gtcaggcgac ggaagatttt gccagcgcgg cggggcggct gaagaccctt ttggagacca
     8101 atgcgcccaa gattgactcc accgtgactt ccgcccggga ggcggcctcc cggatgaatg
     8161 agattactac cggcctggat tcgctctccc attcactcca ttcttttata gacaaaattg
     8221 attcccagca ggggactttg ggcaagttgg tgaacgacga ccggctgtac ggcgaaatac
     8281 gcaaagcggc caacgatctc gacagtctcg tgctggatat taagaaaaac cccaagaagt
     8341 atttacgaat tcagatcttt taggtaatcg ctctgctctc cggtcggaat ttctcttggc
     8401 cgttcccggc gttaagattt ttttgattca aagggaaggc gggacgtacc accgattaat
     8461 ggctctattg ccttccgggt gcggccggtt ctcttggttg cctttgcggc agcgggcgca
     8521 cagggggtaa gaggcggatg aaaagggaaa caaaccccat aagaatttcc gttgtcattc
     8581 cggcgctcaa tgaagtgcaa ttactgcccc attgtctgcg cagtctaaaa aatcagaatt
     8641 ttggccactc gtacgaaatc atcgttgttg acaacaacag cgaagacaga accgccgcgg
     8701 tggctgccgg catgggagcc cgggttgtgt ttgaaccgca tcggggcatt acctgggccc
     8761 ggcagaaagg cctggaagcg gctcgcggag aaattattgc ctgggtggat gccgatacgg
     8821 tagtcaccgt agactggctg cagcaaattt atgccgcctt aaatgatgcc gacggggttg
     8881 ttgccgtaag cggaaaaatt ctgtacacga aaagcactca ctggaaggga aagttacccg
     8941 gctactgggt ggcggctgtc cggtacggtg accgggctat tcgatttttg ctgagaaagc
     9001 ccgggacgct gtgggggggg aattttgcag ttcagaaaaa agcgctgctc caggtcgggg
     9061 gattcaataa gaacattgaa ttttatggag aagatacgga gctgtcttta cgcttgggca
     9121 aggcgggaaa aattcaattc aataagaacc agatcgccta tacttcaccc cggagatttg
     9181 aaaatcaacc gctcatccgg accgcgtggt tttacgtatc caacttcgtt cggataatgg
     9241 tctttcatag atatgccgcg agccggccca agccgggatt tcccgccttg aaaatatact
     9301 ttcagaaagt tctcccggtt ttcggcatgc tctcgatcct tgtcgccttc ggtttctttg
     9361 ctttcaatcc ggcttcacaa ttttacgggc gggtctattc cgcggggtta gactggcgac
     9421 aaaaagtcat cgccctgtct tttgacgacg gcccggatga accctatacc tcgcaggtgc
     9481 ttaacatttt ggatgaaaat ggcattaagg ccacgttctt cataatcggg aagaacgccg
     9541 aatattaccc ggaggccgta agagaaatcg tcgaccgggg ccacattatc ggcaaccatt
     9601 cgtacagcca ttattacctg ctgccgttcg agaacaatga ccgaattcaa aaagacctca
     9661 tcaaaaccga aaatctgctt tttcatttga ccggactgcg gaccgccctt ttccggccgc
     9721 cccacggtct acgcactcc
//
LOCUS       DBNGZA010000085         4186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1500057, whole genome shotgun sequence.
ACCESSION   DBNGZA010000085 DBNGZA010000000
VERSION     DBNGZA010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1500057"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1000
                     /locus_tag="AC16FU_01905"
     CDS             239..1000
                     /locus_tag="AC16FU_01905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR2993165.1"
                     /translation="MAERVRVLFVIVFFVVFGFLASTGYAKVSTSTVSYPSGSDTVSG
                     YLALPGGQGPFPALVVIQEWWGLNDWIKENARKFAEQGYVALAVDLYRGRVANSPDEA
                     HELMRGLPEDRAIRDLKAAAAYLQSRSDVKKNKIGAIGWCMGGGYALQLAINQPDLAA
                     CVMNYGRIVTDSALIADIACPVLGIFGEEDRGIPVADVRQFEKACQRAGKTVNVHFYK
                     AGHAFMNPNNKSGYRPAATKDAWSMITTFFARSLK"
     gene            complement(1301..3085)
                     /locus_tag="AC16FU_01910"
     CDS             complement(1301..3085)
                     /locus_tag="AC16FU_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR2993166.1"
                     /translation="MGRGIKTLRPVIAGAVLMTLAVQSPPAGAQTAKSAALHKIFEDY
                     WEFVLQASPTFATYLGDHRYDDRLEDLSQEFYERRIAKAGEFLARLQAIPLDSLSSAD
                     SLNYDLFAREMQEQIEAAQFRPYLLPLSQQEGPHIGLVELTTYHPFFTPTDYENYIKR
                     LSLFPVQVEQILTNLREGLRTDIVPAKVTVEKIIPQIQAQVVAAPESSDLYQPVRKFS
                     DAFSPDDKVRLTDELKNAIASGVIPAYRKLLDFVQREYLPKCRTEAGIWALPDGDQRY
                     RFAVRHYTTTNLPPEEIFELGKKELARIQAEMKKIKEAAGFKGDLPAFIHYLRTDPKF
                     HYPTADSLVAGFKAILRRMDAKLPLLFGKLPQAHYDFREIERFRAAAAPDAYYYPPPE
                     DFSRPGYFYINTYKPESRPKYTMEALAYHEAVPGHHLQIAIQQELTDLPKFRRHGGYT
                     AFVEGWGLYSEQLPKEVGMYQDPYSDFGRLTMEAWRAVRLVVDPGLHFFKWTREQAIE
                     FFKANTALSEQNIVSEVERYIADPGQATAYKIGQLKIRELRAEAEKTLGPQFDVRAFH
                     DELLSDGALPLAILEKKMQRWVVRVNPN"
     gene            complement(3127..>4186)
                     /locus_tag="AC16FU_01915"
     CDS             complement(3127..>4186)
                     /locus_tag="AC16FU_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR2993167.1"
                     /translation="VEEPGVILVLAPKERSRKEVLLLPPRNPEEERWTGERPSLGKKL
                     EDSLGFEYVQRTTGLGGIINSYLQHTKILSYISPPAAYSSPVPPDLSLYRDVSSRVPE
                     TSIKNFSTLLPSLRVIKSAEEIAAIQKAIDITGAGFRAACQKVKPGMKEEDVQDVIES
                     TFKAEGARRLAFPSIVATGHNTTTLHYTRNDAVVKDGDLFLMDIGAEYDHYAADVSRT
                     IPVSGKFTPEQRKIYDIVLKAQKAAIAKVKPGAFIRDDVHNTARKVIEEAGYGDYFIH
                     STSHFLGMDVHDVGDYSLPLQPGMVITVEPGIYLPDQDIGVRIEDDVLVTENGCKVLT
                     QGIPREVEEIERLLRERK"
BASE COUNT          827 a         1157 c         1271 g          931 t
ORIGIN      
        1 gatctgacgt tcactccgtt tcggtcactg ccggtcaagt atgcaccatt gcagagagtt
       61 tgtaaaggca gagaatctcg accggttcgg gaactgtttg cttttgttct gcaaaatctg
      121 ttttttctga cgtagattga agaaatgggt aaacgataaa aagggagatg tccctactca
      181 gggacgaccg ggggcggtcg ccacacgcga ataacaggtg tagcgcaagg aggagagaat
      241 ggcagaacgt gtccgggtac tgttcgtgat tgtcttcttt gtcgtatttg gttttctggc
      301 aagcaccggt tatgccaaag tcagcacgag cacggtctct taccccagcg gcagcgacac
      361 ggtttcgggc tacttggcgc tgcccggggg ccagggccct ttcccggctc tggtggtgat
      421 tcaggaatgg tggggcttga acgactggat caaggagaat gcgcgaaaat tcgccgagca
      481 gggctacgtg gccctggctg ttgatctcta ccggggcaga gttgccaact cgcccgacga
      541 agcgcatgaa ctgatgcgcg gcctgccgga ggaccgcgcc atccgcgacc tgaaagcggc
      601 ggcggcctac ttgcagagcc ggagtgacgt gaagaagaat aaaatcggcg ccatcggctg
      661 gtgcatgggc ggcgggtatg cactgcaact ggcgattaac cagcccgatt tggccgcctg
      721 cgtgatgaac tacggccgca tcgtcaccga ttcggcgctc atcgccgata tcgcctgccc
      781 ggttttgggg attttcgggg aggaagaccg tggcataccg gtggccgacg tgcgccagtt
      841 cgaaaaggcc tgccagagag cgggcaagac ggtcaacgtg catttctaca aggctggcca
      901 cgccttcatg aatccgaaca acaagtcggg ctaccgcccg gcggcgacca aagacgcctg
      961 gagcatgatt acgaccttct ttgcgcgcag cctcaagtag ccacgatcgg tcgaatacga
     1021 gaagcgattg tgggtagtcg ggctcgtccc tgagcccgac tttaatattt aaatcagcga
     1081 tcctgcggat cgccgaggct aaaactctcg gctacaaata tcaaatcgga attcccacca
     1141 gttccgtagt cgagaattac attctcggcg cccgtccggg cgttgaatta taaaaggcgg
     1201 cggtctaccg gccgcagagg ttaaaaacct cggctacgaa aatccgaatg acccttgggc
     1261 cctgtagccg aggatttcat cctcggcgga gtcaaatggc ttagtttgga ttcacccgca
     1321 ctacccagcg ctgcatcttc ttttccagaa tagccagagg cagcgcgccg tcgctcaaga
     1381 gttcgtcgtg gaaggcgcgg acgtcaaact gcgggcctaa agtcttctcc gcctccgccc
     1441 gcagttcccg gattttcaac tggccgatct tgtaggccgt ggcctgtccg gggtcggcga
     1501 tgtagcgctc cacctcggag acgatgttct gttcggagag tgcggtattg gctttgaaaa
     1561 actcaatcgc ctgttcgcgc gtccacttga aaaagtgcag gcccggatcg acgactaacc
     1621 gcaccgcccg ccaggcctcc atagtcagcc ggccgaaatc ggaataggga tcttggtaca
     1681 tccccacttc tttgggtagc tgctccgagt agagccccca gccctcgacg aaggcggtgt
     1741 agccgccgtg gcggcggaat ttcggcaggt cggttaattc ctgctggatg gcgatctgca
     1801 ggtggtggcc gggaaccgct tcgtgatagg ctaaggcctc catggtatac ttgggccggc
     1861 tctccggttt gtaggtgttg atgtaaaagt agccgggccg ggagaaatct tccggcggcg
     1921 gatagtagta ggcgtccggt gctgccgcgg cgcggaaacg ctcgatctcc cggaagtcgt
     1981 agtgcgcctg gggcagtttt ccgaaaagca agggcagttt ggcatccatc cgccgcagaa
     2041 tcgctttgaa cccggccacg agcgagtcgg cggtcgggta gtggaacttc ggatctgtcc
     2101 gcaggtagtg aatgaacgcc ggtaaatcgc ccttgaatcc cgctgcctcc ttgatctttt
     2161 tcatttccgc ctgaatgcgc gccagttctt ttttgcccaa ttcaaaaatc tcttccggcg
     2221 gaagattggt ggtcgtgtaa tgccggacgg cgaagcggta gcgctggtcg ccatccggca
     2281 gggcccagat gcccgcctcg gtgcggcatt tggggaggta ctcgcgctgc acgaaatcga
     2341 ggagtttccg gtaggcggga atgaccccgc tggcgatggc gttcttcagc tcgtcggtga
     2401 gccggacttt atcgtcgggc gaaaaagcat ccgaaaattt cctgaccggc tggtagaggt
     2461 cgctcgattc cggtgcggcc accacctgcg cttgaatctg gggaataatt ttctccaccg
     2521 tcaccttggc cggaacgatg tcggtgcgca gaccttctcg cagattagtt aaaatctgct
     2581 cgacctgcac cgggaatagg gagagccgtt tgatgtagtt ctcgtagtcg gtgggtgtga
     2641 agaaggggtg gtaggtggtg agttccacca gcccgatgtg cggtccctcc tgctgggata
     2701 gcggcaagag gtagggccga aattgggccg cttcgatctg ctcctgcatt tcacgggcaa
     2761 agaggtcgta gttgagactg tcggcggatg acagcgaatc gagcggaatt gcctgcagcc
     2821 gggccaggaa ctctccggcc ttggcgatcc gccgctcgta gaattcctgc gagaggtctt
     2881 ccagccggtc gtcgtagcgg tgatcaccca agtaggtggc aaacgtcgga gacgcttgca
     2941 ggacaaattc ccagtagtcc tcgaagattt tgtggagtgc tgccgatttg gcggtctgcg
     3001 ctccggctgg cggagattga actgccaaag tcataagcac cgctccggcg atgaccggac
     3061 gaagagtctt gattcctctg cccataatac ctcgcttcgc aggttgaaat tcctcaaaac
     3121 tatcgattac ttccgttcac ggagaagtcg ttcaatctct tctacctcgc ggggaatgcc
     3181 ttgggtgagc actttgcacc cattttccgt caccaaaacg tcatcttcga tccgcacgcc
     3241 gatgtcctga tccggcaggt aaatccccgg ctccacggtg atcaccattc ccggttggag
     3301 cgggagcgag tagtcgccca cgtcgtgcac gtccattccc aaaaagtggc tggtgctgtg
     3361 gatgaagtag tcgccgtagc ccgcctcttc gatcaccttg cgggcggtgt tatgcacgtc
     3421 gtcgcggatg aaggcacctg gtttgacctt ggcaatggcc gccttctggg ctttgaggac
     3481 gatgtcataa atcttccgct gttccggcgt gaacttgccc gacaccggga tggtgcgcga
     3541 cacgtccgcc gcgtaatggt cgtactccgc cccgatgtcc atcaagaaca ggtcgccgtc
     3601 tttcaccacc gcatcgttgc gggtgtagtg cagggtggtg gtgttatgtc cggtggcgac
     3661 aatcgacggg aacgccagcc gccttgcccc ttcggcctta aaagttgact cgataacgtc
     3721 ctgcacgtcc tcttctttca ttccgggttt gaccttctgg caggcggctc ggaatcccgc
     3781 gccggtgatg tcgatggcct tctggatcgc ggcaatttct tcggctgatt tgataacccg
     3841 caaagaaggc aaaagcgttg agaagttttt gattgacgtt tccggcacgc gggaggagac
     3901 gtcgcggtag agcgagaggt cgggcggcac gggcgaactg tacgcagccg ggggcgaaat
     3961 gtacgagagg attttcgtgt gctggaggta ggagttgatg atcccgccta aacccgtcgt
     4021 cctttgcacg tactcaaagc caagcgaatc ttctaatttc tttcccagag acggccgttc
     4081 gccggtccag cgctcttctt ccggattgcg cggcggaagc agaagcactt ccttccggct
     4141 ccgctctttc ggggccagta ccagaattac ccccggctct tccact
//
LOCUS       DBNGZA010000086         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_969852, whole genome shotgun sequence.
ACCESSION   DBNGZA010000086 DBNGZA010000000
VERSION     DBNGZA010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_969852"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..104
                     /locus_tag="AC16FU_01920"
     CDS             9..104
                     /locus_tag="AC16FU_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993168.1"
                     /translation="MQTGMIIGAALCEALTIYSLIAFFMLQGRIK"
     gene            179..664
                     /gene="atpF"
                     /locus_tag="AC16FU_01925"
     CDS             179..664
                     /gene="atpF"
                     /locus_tag="AC16FU_01925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR2993169.1"
                     /translation="MNLELQQILTQIVGFLIALWILKRYAWKPLLNLLEQRRAKIRSE
                     FDAIEEQKKKIDAIAADYREKLKEIDALARVRLQEAVAEGQKVAVEIRENARREAKEL
                     LDKAKAELEREVAKAKVQLKNDLVEMTLAASRKMVKESLDTAKHRQLISDFISDLERV
                     T"
     gene            664..1230
                     /gene="atpH"
                     /locus_tag="AC16FU_01930"
     CDS             664..1230
                     /gene="atpH"
                     /locus_tag="AC16FU_01930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR2993170.1"
                     /translation="MARTKRTGDAALAERYAAALFALAQEKGAVEPVAADVAGLVELF
                     DRDSTLEDFLLAPHVLDEQKEELARTALSGKVLPTFLDFLLLLLDRHRFDQFRAIARR
                     YQELMEEDQGILRAQVVTVVDLPPVEALRLKAKLEQKTKKKILLKPVVDPEILGGMIV
                     KLKHQIIDRSIAQQLVELKEQLLAVKVH"
     gene            1327..>2524
                     /gene="atpA"
                     /locus_tag="AC16FU_01935"
     CDS             1327..>2524
                     /gene="atpA"
                     /locus_tag="AC16FU_01935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR2993171.1"
                     /translation="MAIRPEEVSAIIKKEIERYETKLEMESVGTVLQVGDGIARVWGL
                     EDAMMSELVLFPGEVTGMVLNLEADNVGVALFGSDRGIKEGDTVRSTGRIAQVPVGEA
                     LIGRVVNALGQPLDGKGPIVTDKFRPMEGHAPNVVQRQPVNQPVQTGLKAIDSMIPIG
                     RGQRELVIGDRQTGKTALVLDTIINQKGKDLFCIYVAIGQKQSTIAQVVETLTGYGAM
                     EYTTVVAAAATDPAPLQYISPYAGCAIGEEFRDIGRHALCVYDDLSKHAQSYRQLSLL
                     LRRPPGREAFPGDIFYLHSRLLERAAKLADQRGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYAGVRPAINVGISVSRVGGKAQVKAMRKVAGRLRLDLAQYREL
                     AAFAQFG"
BASE COUNT          577 a          695 c          781 g          471 t
ORIGIN      
        1 cgggcaagat tcaaaccggc atgatcatcg gcgcggcgct ctgcgaggcg ttgacgattt
       61 actccttgat cgccttcttc atgctgcagg ggaggatcaa gtaaaaaggt ggtaggtgga
      121 aggtggtagg tggtggggaa atatcatccg ccgccaatgc acttcctggt gaacgacgat
      181 gaaccttgaa ctgcagcaga tcttaaccca gatcgtcggc tttctcatcg cgctctggat
      241 tctcaagcgc tatgcctgga aaccgctttt gaacctgctc gagcaacggc gggccaaaat
      301 cagatccgag ttcgacgcca tcgaagaaca gaaaaagaaa atcgatgcca ttgccgccga
      361 ttaccgggag aaattaaaag agatcgacgc gctagcacga gtacggctgc aggaggcggt
      421 cgccgaaggc caaaaagtcg cggtggaaat tcgcgagaac gcgcggcggg aagcgaaaga
      481 acttttggac aaggccaaag ccgaattgga gcgcgaggta gccaaagcca aggtgcaact
      541 gaaaaacgac ctggtggaaa tgactttggc cgccagccgg aaaatggtca aggagagtct
      601 ggataccgcc aagcaccggc agttgatttc cgatttcatt tccgatctgg agcgggtaac
      661 ctgatggcgc gcaccaaacg caccggggac gcggccttgg ccgaacgata cgccgccgca
      721 ctctttgcac tggcacaaga gaaaggtgcc gtcgaaccgg tcgcggctga cgtcgccggt
      781 ttggttgagc tcttcgaccg cgattcgact ttggaagatt ttctcttggc gccccacgtc
      841 ttagacgaac agaaagagga attggcgcgg acggcgcttt ccggaaaggt gttgcccacc
      901 tttctcgatt tccttctgct ccttctggat aggcaccgct tcgaccaatt ccgggccatc
      961 gcccgccgtt accaggagct gatggaagag gatcagggca tcctgcgagc gcaggtggtg
     1021 accgtggtcg atcttccgcc ggtggaagcc ctccggctga aagccaaact cgaacagaag
     1081 acaaaaaaga aaattctgct caaaccggtc gttgatcccg aaatcttggg cgggatgatc
     1141 gtcaaactca agcaccagat catcgaccgg agtattgcac agcaattggt tgaactcaaa
     1201 gaacagcttc tggcagtcaa agtgcattag aaaatcagca ttgaggactg agaattgagg
     1261 gaaggccaaa atcccgaata ggtcaggcat cttgcctgac cgcgagcact gaacggagac
     1321 cagtaaatgg cgatacggcc ggaagaagtc agcgcgatca tcaaaaaaga aattgaacgg
     1381 tacgagacca aactcgaaat ggagtcggtg gggaccgtgc tccaggtggg ggacggaatc
     1441 gcccgggtct ggggtttgga agacgctatg atgtctgaat tggtgctctt ccccggtgaa
     1501 gtcaccggca tggttttgaa cctcgaggcc gacaacgtgg gggtggcgct cttcggctcc
     1561 gaccggggca tcaaagaggg cgatacggtc cggagcaccg ggcgcatcgc gcaggtgccg
     1621 gtgggggagg cgctcatcgg ccgggtggtg aacgcgctgg gccagcccct ggacggcaaa
     1681 gggccgattg tcaccgacaa attccggcca atggaaggcc acgcgccgaa cgtcgtccag
     1741 cgccagccgg tgaaccagcc cgtgcaaacc gggttgaaag caatcgactc gatgattccc
     1801 atcggccggg gccagcgcga actggtgatc ggcgaccggc agacgggaaa aacggcgctc
     1861 gtcctagata ccatcatcaa ccaaaagggt aaagacctct tttgcattta cgtcgccatc
     1921 ggacagaagc aatcgaccat tgcgcaggtg gtggagaccc ttacgggcta cggcgcaatg
     1981 gagtacacca ccgttgtggc ggctgcggcg actgacccgg cgcctttgca gtacatttcc
     2041 ccgtacgcgg gctgcgccat aggcgaggag ttccgcgaca tcggccgtca tgccctctgt
     2101 gtctatgacg atctttcaaa gcatgcgcag tcgtaccggc agctctcgct cctgttgcgc
     2161 cggccgccgg ggcgggaggc cttcccgggc gacatcttct atttgcactc ccgcttgctc
     2221 gaacgggcag cgaagttggc tgaccagcgc ggcggcggct ccttgaccgc cctgccgatc
     2281 atcgagacgc aggcggggga tgtgtcggct tacattccga ccaacgtgat ttccatcacc
     2341 gacggccaga ttttcttaga gtcggatctc ttctacgccg gggtgcggcc ggcgattaac
     2401 gtcggcatat cagtttcccg cgtcggcggc aaagcccagg tcaaggcgat gcggaaagtg
     2461 gcgggccggt tgcggctcga tttggcccag taccgggaac tggcagcctt cgcccagttc
     2521 ggct
//
LOCUS       DBNGZA010000087         4674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2349988, whole genome shotgun sequence.
ACCESSION   DBNGZA010000087 DBNGZA010000000
VERSION     DBNGZA010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2349988"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..635
                     /locus_tag="AC16FU_01940"
     CDS             78..635
                     /locus_tag="AC16FU_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2993172.1"
                     /translation="MKTKQSNFDSAWWGGRAPGLTVVLLGFLFALGTSAFAADQRKAT
                     GSQLSPVQESKPAEARATAPLQIELECLSVQGSGATFVTGGGLQLGFSVAQSVAGAQS
                     AATRKLGIGFWGACAACALLGDLNGDLVRDIVDIVAMINYVVFGDPVATGLICADLTG
                     DGVVDISDIICLINNVVFSNPIPCL"
     gene            complement(708..1757)
                     /locus_tag="AC16FU_01945"
     CDS             complement(708..1757)
                     /locus_tag="AC16FU_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993173.1"
                     /translation="MVGIDSYRNNNLWLKSRAWDLSLLTFSGVLSIFPHALYSWMGFK
                     PWMVGLTVALLVGGPHIYATFTRILLDKSFTQRFSNYIFIVFAVIPVAVVYLLITRQV
                     LLLTIFFFWASMHVLHQIAYVVELYNQKMPVRIPNIRRAAEYFLVFTSLYPFAISKLV
                     SGNFYIGTKKLLFPQFMAGPAIPILVWTVFGLSLLVVIYHSLRDWIRERRIYPKTYLL
                     GVTVVVCGIAPTFPDLDVAFQGVNIWHSFQYLALTYYITRLKKAEGLLREKPVARVIE
                     KRQGLNFYLFHVGITLLAGVAVVALYKFSGLSWQMAYWAVIFSSLLVHYFFDHILFTE
                     FRLFKTRLKAEPALA"
     gene            2182..2319
                     /locus_tag="AC16FU_01950"
     CDS             2182..2319
                     /locus_tag="AC16FU_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993174.1"
                     /translation="MRRHRSPALLFWIAIGLTLLAILLTYSDMDGQTATSTAKSLISL
                     F"
     gene            complement(2441..3220)
                     /locus_tag="AC16FU_01955"
     CDS             complement(2441..3220)
                     /locus_tag="AC16FU_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster loop"
                     /protein_id="HIR2993175.1"
                     /translation="MAQPSIRDILIKRGEEILSQPFRPIVFARHQEADALLNDLDRYP
                     HAFVLACIMDRQIIAEKAWMIPYEFKERLGSFEFTNLQALSLERVQRIMSNPKPLHRF
                     PIVMSKNFFSAVQKIARVYSGKASNIWAGCPDSVTIVNRFREFDGVGPKIGPMAANIL
                     VRDFKIPIGNKNSIDISPDVHVRRVFTRLGLIVEGASNEELIFRARELNPTYPGVFDL
                     PAWEIGREWCKPNKPNCPKCYMNGCCPTARITKGGKTGRNA"
     gene            complement(3377..>4674)
                     /locus_tag="AC16FU_01960"
     CDS             complement(3377..>4674)
                     /locus_tag="AC16FU_01960"
                     /inference="COORDINATES: protein motif:HMM:NF044439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993176.1"
                     /translation="EDLAGRGLLTQHDNGSIAATSFGKKVAQTGIRVATGKAIEEACS
                     SKTRFDALDWFYFLCGTEEAANLPVTLNFHERTSDYYHGLWLKEKTNFPPDSGPLVSG
                     QMVVRLKLALLLSDWIDGVVTFDLEKKFFSPAGTIQQVAETLEWLLDAAAQMALVLCP
                     GQKLSPELKSLSRRVKWGAREELLPFFDALGPAAGRDHLLKLVASGWKTIEGLKQATV
                     EELRGVVPQKIAERIVAHLERPQAGAPAKEKSHPARAVPEQGGNQNPRKAGLWLVGVP
                     KKQRWTVEIDGQPVLLSNKSFRFLALLAFARVERPDGWLSKEELEPGDYQARYLFQLK
                     REIAEQLGVRKCRGESLDSPKNAGEPKGSPLHATGRERPNMIDIENDRRGHYRLALKP
                     EEIDFDFATLAKHPDVEIKKGATRLSTYCPNKMNATQKE"
BASE COUNT         1067 a         1210 c         1350 g         1047 t
ORIGIN      
        1 gcagaggtgg cagagatgaa ggcgcgcctg cagacgctgg aagagcaggt gaagcagcag
       61 aacgcgggag ggggccgttg aaaacgaaac agtcgaattt cgattcggcg tggtggggcg
      121 gcagagcgcc gggcctgact gtcgttctgc tcgggtttct cttcgctctc ggtacttccg
      181 cgttcgctgc cgatcagcgg aaggcaaccg gttcgcaact ctcgccggtg caggagagca
      241 agcccgccga agcccgcgca accgcaccgc tccagattga actggagtgc ctttcggttc
      301 agggaagcgg ggcgaccttc gtcaccggcg gtgggctgca gttggggttt tcggtggccc
      361 agtcggtggc aggcgcacag agcgcggcca cgcgcaagct cggcatcggg ttctgggggg
      421 cctgcgcggc ctgcgcgctt ttgggtgatt tgaacggcga tttggtgcga gacatcgtgg
      481 acatcgttgc catgatcaat tacgtcgtgt tcggggaccc ggtggccacg ggtctcatct
      541 gcgccgattt gaccggcgac ggcgtggtgg acatcagcga catcatctgc ctgatcaaca
      601 acgtcgtttt cagcaacccg atcccctgct tgtagaagaa aagagcagca atcgaaagag
      661 cgcccctgcg ggcgctcttt tttttctgcc caattcgggt gcgcgagcta cgccagggcc
      721 ggctcggctt tgagccgggt tttgaacagc cggaactccg tgaataggat gtggtcgaaa
      781 aagtagtgaa ccaggagcga ggagaaaatc accgcccagt aggccatctg ccaggagagg
      841 ccggaaaatt tatagagcgc caccaccgcc accccggcca gaagggtgat gccgacgtgg
      901 aagaggtaga agttcaaccc ctggcgcttc tcgatcacgc gggccaccgg tttttcacgc
      961 aggaggcctt cggctttctt gagccgcgtg atgtaatagg tcaaggccag gtactggaac
     1021 gagtgccaga tgttgacgcc ctggaaggcc acgtccagat cggggaaagt gggagcgatc
     1081 ccgcagacga cgaccgtcac gcccagtaag taggtcttgg ggtagatgcg tctctcgcgg
     1141 atccaatccc gcagggagtg gtagatgacg accaataaac tcaagccgaa gactgtccag
     1201 accaaaatgg gtatggcggg tccggccata aactgcggaa agagcagttt tttcgtgccg
     1261 atgtaaaaat taccgctgac cagtttggaa attgcaaagg ggtagagact ggtgaagacc
     1321 aaaaagtact ccgccgcgcg ccttatgttc gggatccgca ccggcatttt ctggttgtag
     1381 agttcgacga cgtaggcgat ctggtgcagg acgtgcatcg aggcccagaa gaaaaagatg
     1441 gtcaggagca agacctgccg ggtgatgagc aggtagacga cggcgacggg gatgacggcg
     1501 aagacgatga agatgtagtt tgagaatctt tgggtgaaac tcttatccag caaaatgcgg
     1561 gtgaaggtgg cgtagatgtg cggccccccc accaaaagcg ccacggtcag ccccaccatc
     1621 cagggtttga aacccatcca ggagtagagg gcgtggggga agatcgagag gacaccggaa
     1681 aaggtgagga gcgacaggtc ccaggcccgg ctcttcagcc acaggttatt gtttcggtac
     1741 gagtcgattc cgaccaaagt aatcctccgt gacagcgcga agagaaatca tcagcaattg
     1801 cactacccta cggttgactg acccaagtcc ccgaaaaaag taagccggta tatacggcgg
     1861 aatgcaaagc gttgtcaagg aaaaattggt atgggaaagt tcccatactg ttggcgaccc
     1921 gtacgggtcg ccctacgcaa gaagtcggga ataataaagc cgcgtaggtg cggcgccctc
     1981 gccgcacttg aattatggag atgacggcgc tatcatcttg agcaaagaaa ttcacagtcc
     2041 cttttccggg cgggtattcg ggtcaaaaac ctcgttggta gatcacaaga tagcgggagg
     2101 aggcgacggg tagtttggca cacccgttgc agaaagggtg ggtgtgcaga aaccaccgac
     2161 tccattctgg agagtcacca cgtgagaagg caccgcagcc ctgcgctctt attctggatt
     2221 gccatcggcc tgacgcttct ggcgattctt ttgacctaca gcgacatgga cggtcaaacc
     2281 gccaccagca cggccaagag cctcatctct ctcttctaag tcctgtcacc catacgggtc
     2341 gggataggtt tgaggggggt acctcaacaa ggcgaccagg cggtcgcgct cgattacgag
     2401 cggggccgct ttcctctttt atgaatagtt aaagccgacc tcaagcattt cgccctgttt
     2461 tcccgccttt agtaatcctt gcggtaggac aacacccatt catatagcat tttgggcagt
     2521 ttggcttatt tggtttacac cattctctgc caatttccca ggccggaagg tcgaagactc
     2581 cggggtaggt cgggttcagt tcccgggcgc ggaagattag ttcttcgttt gaagcgccct
     2641 ccactatcag accgagtcta gtgaatactc tccgaacatg gacgtcagga gaaatgtcaa
     2701 tggagttctt gttgccaata ggaatcttga agtcgcgcac gagaatgttt gccgccatcg
     2761 gtccaatttt ggggcccacg ccgtcaaact cgcgaaatcg gttaacaatg gtgacactat
     2821 caggacagcc agcccatata ttagatgcct tacctgaata cactctcgca atcttttgca
     2881 ccgcagaaaa aaaattcttg ctcatgacga tcgggaatct gtggagaggt tttggatttg
     2941 acattattct ctgtactctt tcgagagata atgcttgaag attggtgaac tcaaaagaac
     3001 cgagtcgttc tttgaactca tagggaatca tccacgcttt ttcagctata atctgacgat
     3061 ccataataca ggcaagtacg aatgcatgag gatagcgatc tagatcattg agaagtgcat
     3121 ccgcttcttg atgtcttgca aagactattg gtctaaaagg ttgcgagagg atctcttcgc
     3181 ctcttttgat caagatgtct ctgatagacg gctgtgccat tttttctcca agttattgtg
     3241 aggccccgtt acggctttag gtagaagtgt ttttttgtca tagcccgaca atgcaaatat
     3301 gcatcttttt tgtctaaggg caaaatggat gattagcata tcttcctctt aactcaagtt
     3361 ttgtcacatt agaagattac tccttttgag tcgcgttcat tttgttagga caatatgtag
     3421 agagtctagt tgcccccttc ttaatttcca catccgggtg tttggcgagc gtggcgaaat
     3481 cgaagtcgat ctcttccggc ttgagcgcca gccggtagtg gccgcggcgg tcgttctcga
     3541 tgtcaatcat atttggtctt tcacgaccgg tggcatgtag gggtgaacct ttaggttcac
     3601 ccgcattctt gggcgaatct aaagattcgc ccctacattt tctcacacct aactgctcag
     3661 cgatttcccg cttcaactgg aacaggtagc gcgcctggta gtcgccgggc tccaattcct
     3721 ctttggagag ccaaccgtcc gggcgctcga cgcgggcgaa ggccagaagc gcgaggaagc
     3781 ggaacgattt gttcgaaagc aaaacaggct ggccatcaat ttctacagtc cagcgctgtt
     3841 ttttggggac gccgacaagc caaagccctg cttttcgcgg attttgattc cctccctgct
     3901 ccggtacggc tcgcgccggg tgggattttt cttttgcggg cgcgccagct tgcggccgtt
     3961 cgagatgggc cacaattcgc tccgcgattt tttgcgggac aacacccctc aattcctcga
     4021 cggtggcttg cttgagaccc tcaatcgttt tccagccgga agcaaccagt ttcagcaggt
     4081 ggtcgcggcc ggcggccggg ccgagagcat caaaaaacgg cagcaattcc tcccgcgcgc
     4141 cccatttgac ccggcgcgaa agcgatttga gttccggtga caatttctgg ccgggacaga
     4201 gcaccagcgc catctgcgcg gcggcatcga gaagccattc cagcgtttcg gccacctgct
     4261 gaatcgttcc ggccggagaa aagaatttct tctccagatc gaaggtgacc accccgtcga
     4321 tccaatccga aagtaaaagc gccagtttga gccggacgac catctgaccg gaaacgaggg
     4381 gcccggaatc gggagggaaa ttcgttttct ctttgagcca gaggccgtgg taataatcgg
     4441 aggtgcgttc gtgaaagttc agagtgacgg ggaggttcgc cgcttcctcg gttccgcaga
     4501 gaaaatagaa ccagtctaaa gcgtcaaatc gagttttcga agaacaggcc tcttctatcg
     4561 ctttgccggt ggccacgcga atgccggtct gggccacttt tttgccaaaa gaggtggccg
     4621 caatgctgcc gttgtcgtgc tgggtcaaaa gaccacgccc agcaaggtcc tcaa
//
LOCUS       DBNGZA010000088         3332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2137198, whole genome shotgun sequence.
ACCESSION   DBNGZA010000088 DBNGZA010000000
VERSION     DBNGZA010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2137198"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16FU_01965"
     CDS             complement(<1..337)
                     /locus_tag="AC16FU_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993177.1"
                     /translation="MDSVPLLSLPASLKPHTAALDSSGGAIRLSNGSPDDLLLLDLSA
                     YRNWRSQTTYLDEWGKLKATSIHKPESQKAGGLTHFQIPWKPPKAVANIIGEDSAGLK
                     VSGYRRIPMG"
     gene            complement(595..1590)
                     /gene="murB"
                     /locus_tag="AC16FU_01970"
     CDS             complement(595..1590)
                     /gene="murB"
                     /locus_tag="AC16FU_01970"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR2993178.1"
                     /translation="MLKLQEGTQRDGPLTPPWAGLLKKYLSEEVTEKVLLAPHTTFGL
                     GGPADFFYAPRTLTEVARAVQLAKDEGIPYYLMAGGSNLVIADRGFRGLVIKITCAEL
                     EADNHTISAQAGISLGKVVDTAYEMGWAGIETLTGIPGSFGGAIYGNAGAYGRSISDV
                     LESAVVYDSKGQVKIVGPDYFGFRYRHSDLKVTKEVVLSARIRLTPGDKPKLKAKMEE
                     ILATRHAKHPVDLGSAGSYFKNIEDPQAEFGKVPAGLLLDRVGAKNIRIGNAGVYRRH
                     ANIIVNYGGALTSDVLRVAELMREKVKRAQGITLEEEIQLVGDFDEPPAGILKAA"
     gene            complement(1687..>3332)
                     /locus_tag="AC16FU_01975"
     CDS             complement(1687..>3332)
                     /locus_tag="AC16FU_01975"
                     /inference="COORDINATES: protein motif:HMM:NF028259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative ABC transporter permease subunit"
                     /protein_id="HIR2993179.1"
                     /translation="LLKVRFKSYLNQFRRVSWGKRIGALALLLAYLYVGVMLSLLFVA
                     LFKLFQRPELASLGLDYHMLGALFLIVLVALLFSGNSFTIYTIFLSPDLPLLSTFPLA
                     RRTIFTSKFLEGTALNALIALVFLWPVMLGFGLAHRVTFVYYPVALAGLFLLVLVPTG
                     LSVFTALFFIRILPVTRIKEIMAAAGSILGIAIWVGSQLVSQRAEHAFSGTQAYKTVE
                     SLRPLLDRPWFDYLPSHWMAKTLCGIAGLDFPVLFPAFLTVTFATLFFFGCLLLAERV
                     YYRGLSALQSEAVRQRKRKKPRTIAIPLPDRVRSPLWAIAIKDLRTTFRNFNLLINIA
                     IFPLMVILMPVLMSQNQKMPSGIGHLVQYFLIFFLLIMAASQMGMRTVPLEGKGFWLL
                     LTSPLALEKILWSKLWSSLFLVMLIAVAGLVSLGLLRGVDVTFSLFTAVASLCAAAGA
                     ASLGIWQGAAYANFDWTNPKRMIKGAGGITFVFINLGYIAVWAVIIGLTYLAMKTLHL
                     PVWLAAVVLALLGISVAWALTMLSIKRAAVSIAKREWEY"
BASE COUNT          780 a          942 c          951 g          659 t
ORIGIN      
        1 cacccatggg aatccggcgg tagccggaga ctttcagccc ggcagagtcc tcgccgatga
       61 tgttcgccac cgccttgggc ggtttccagg gaatctggaa gtgggtcagg ccgcccgcct
      121 tctggctttc gggcttgtgg attgaagtcg ctttcagttt gccccactcg tccagatagg
      181 tcgtctggct gcgccagttg cggtaggcgg agaggtccaa caaaagcaga tcgtccggtg
      241 agccgttgga gaggcgtatg gcgccgccgg acgaatccag agcagccgta tgcggcttta
      301 agctcgccgg cagggaaagg agcgggactg aatccaaaca ccagcagagg cccgtccggg
      361 gggtcggtcc ttgggacggg acagagccat cgacggcgag gtccaggcgc agtccctggc
      421 caaaacagag gaggggccac cccaagaaaa acaaaaacgc caaccacgcg ctttggcgcc
      481 gcctgttggc gaagattttg gtcacgttcg gcaaacgact aacccttttt agtcgatcgt
      541 aacttttcgc cttcggtctt tatttcctcc tttcgctcgg gttgactgtc gccatcaggc
      601 cgccttgagg atacccgcgg gaggctcgtc aaaatcaccg acgagttgaa tttcctcctc
      661 cagagtaatc ccctgcgccc gtttcacctt ttcccgcatc agttcggcca ctctgagcac
      721 gtcactggtc aaggcgccgc cgtaattcac gatgatgttc gcatgccgcc ggtagacccc
      781 ggcattgccg atgcgtatat tttttgcgcc aactcggtcc aagagcaatc cggccgggac
      841 cttgccgaac tcggcttgcg ggtcctcaat gttcttaaaa taactccccg ccgaaccgag
      901 gtcaaccgga tgtttggcgt gtctggtcgc cagaatttcc tccatcttgg ctttcaattt
      961 tggtttgtcc ccgggcgtca accggatccg cgccgacagc accacttctt tggtcacctt
     1021 caggtcacta tgccggtagc ggaagccgaa gtagtcgggc ccgactattt tcacctgccc
     1081 tttactatcg tacaccactg cgctttccag aacgtcgctg atcgaccggc cgtaggcgcc
     1141 ggcatttccg taaatggcac cgccaaaact gcccggaatt cccgttaagg tctcgatgcc
     1201 ggcccagccc atttcgtagg ccgtatcgac cactttgccg agcgaaattc cggcctgggc
     1261 gctgatggta tgattgtcgg cttctaactc cgcacaggtt atctttatca ccaatccccg
     1321 gaagccccgg tcggcaatca ccagattgct cccgccggcc ataagatagt agggaatccc
     1381 ctcatctttc gcaagttgaa cggcgcgagc gacttcagtc aaagtccggg gtgcgtagaa
     1441 gaagtcggcc gggccgccca ggccaaaggt ggtatgaggg gctaagagta ccttttcggt
     1501 tacctcctca cttaaatact ttttcagcag ccccgcccac ggcggggtca gaggtccgtc
     1561 tctttgggta ccttcctgca gtttcaacat ataatatacc cagaatcagg cgcctgtcaa
     1621 ggattttcgt cgtttttctc gctcgtgcct cagggtgagc gctgcttaaa ctaccgtatc
     1681 aaatgcctaa tattcccact ctcttttggc tatcgacacg gccgcccgct ttattgagag
     1741 catagtcaag gcccaggcga ctgagatgcc caggagagcc aggacgaccg cggccagcca
     1801 gaccggcagg tggagggttt tcatcgccag ataggttaaa ccgattatca cggcccagac
     1861 cgctatataa cccagattga tgaagacgaa agttatcccc ccggctcctt taatcatccg
     1921 cttggggttc gtccagtcaa agttggcgta ggcagccccc tgccagattc ccagagacgc
     1981 agccccggct gcggcgcaga gggaagccac agcggtgaaa agactaaaag tgacatccac
     2041 accgcggagc aacccaaggg aaaccagtcc cgccaccgca attagcatga cgagaaaaag
     2101 ggacgaccaa agtttactcc agaggatttt ttccaatgcg agggggctgg ttaaaaggag
     2161 ccagaaccct ttgccttcta ggggaacagt acgcatgccc atctggctgg cagccataat
     2221 cagcaggaag aaaatcaaaa aatactgcac gaggtggccg attcccgaag gcattttctg
     2281 gttttggctc atgagcaccg gcatcaagat gaccatcagg gggaagatgg cgatgttgat
     2341 taaaaggttg aagttgcgga aagtcgtccg gagatcttta atggcaatag cccacaacgg
     2401 gctgcgtacc cggtcaggaa gcgggattgc aatcgttcgg ggttttttgc gcttcctttg
     2461 acggactgcc tctgactgca acgccgataa cccgcggtag tagacccgct ccgccagaag
     2521 caggcaaccg aaaaagaaga gcgtcgcaaa ggtcactgtc aaaaaagcgg ggaaaagcac
     2581 cgggaaatcg aggccggcga ttccgcagag cgtcttggcc atccagtggg atggcaggta
     2641 gtcgaaccag ggccggtcca gaagcggcct gagcgattca accgtcttgt aggcctgtgt
     2701 gccggaaaag gcgtgctccg cacgctgaga caccaactgg gaaccgaccc aaattgcaat
     2761 tcccaaaatt gaccccgcgg cggccatgat ttctttgatg cgcgtgaccg gcagtatccg
     2821 gatgaaaaag agcgcggtga ataccgagag gccggtcgga accagcacga gcaggaagag
     2881 ccccgcgagc gcaaccggat agtaaacgaa cgttaccctg tgcgccaggc cgaagcccag
     2941 catcaccggc cagaggaaaa ccagggcgat cagggcattc aatgcggtgc cttcgagaaa
     3001 tttgctggtg aaaatcgtgc ggcgcgccaa ggggaacgtg gagagcaggg gcaggtcagg
     3061 ggagaggaaa atcgtataga tcgtaaaaga gttgccgctg aagagcagag cgaccaatac
     3121 gatcaggaaa agagctccca gcatgtggta gtccagccca agagacgcca gttccggacg
     3181 ctggaagagc ttgaacaagg ccacgaagag aagagagagc atcacgccca cgtacaggta
     3241 ggcgagcagg agggccaaag cgccaatcct tttgccccag gaaacgcggc ggaattgatt
     3301 gaggtaactt ttgaatctaa ctttgaggag ca
//
LOCUS       DBNGZA010000089         3444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2560618, whole genome shotgun sequence.
ACCESSION   DBNGZA010000089 DBNGZA010000000
VERSION     DBNGZA010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2560618"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..758
                     /locus_tag="AC16FU_01980"
     CDS             300..758
                     /locus_tag="AC16FU_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2993180.1"
                     /translation="MFNFVSPRGIPLVGFWPTGKDLEKLEKPLSYQPTDPPLLSMLDA
                     SALYQKSDVIFIDARSPADFEKGHIPRAINVPFEEVDLEEFWPKVADRVPKEKQVVTY
                     CGGADCEASLHLARFLKEKGYSNLHIFFGGWKHWTESKMPIEQGPVASSQ"
     gene            782..1273
                     /locus_tag="AC16FU_01985"
     CDS             782..1273
                     /locus_tag="AC16FU_01985"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HIR2993181.1"
                     /translation="MEKILGNRWLQIMLRLFLGGVFIYASVDKIAHPDQFARAIYNYR
                     ILPGELVNLLAIWLPWLEIATGAFLIFGVWLRASALLLTVQIAMFFIAASSALARGLN
                     FACGCFTTDPKAQSAGITHLFLDGGLLLIALVILFFARRPRAGTEPITVPATAAPEPV
                     SGG"
     gene            1270..2160
                     /locus_tag="AC16FU_01990"
     CDS             1270..2160
                     /locus_tag="AC16FU_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR2993182.1"
                     /translation="MNSRWKNLFGWSKSKADTWERCKKQFFYEYAAQYEAEPLGAQAK
                     SLKKLTKIPFLKGNLIHEAIAAEIQRRRQGQAVNPERAKAYARTRITEVTAQPKRFLS
                     EAVNGLSVTAQLPKAEEEVIQEIETFFSSIWPRYEEWEVLYVDGEEDFSSFVYGTAKV
                     FAEPDLVTKNPDGTCLVTDWKTGSEWEEPEESEELSAQILWVERRYKLLLPQITAEYV
                     YLRTGQVRETRRTLEQMEKFVTRFIRSTSEMLQAENESDLPPTPSLKNCLDCKFATIC
                     EEGRPFLPSEPAVETTLLNS"
     gene            2637..3444
                     /locus_tag="AC16FU_01995"
                     /pseudo
     CDS             2637..3444
                     /locus_tag="AC16FU_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
BASE COUNT          787 a          930 c          999 g          728 t
ORIGIN      
        1 gtcgccagcc tgtgccggca ggtgggcttc cgcgaggcca cctaccagaa tctcatgggc
       61 ggctcggtgg cggtctaccg gatggtgaag tgattcaagt gaagcgacgc tgggattgtg
      121 cagaatcgag gcattttgca tctttccagt ggaggttttg gagactaacg taagagacgc
      181 tgataccgta atttttttgc atgattattt tttaggagcg cgagcggatg actgaatagt
      241 ggagtaaggt tatcaatcgg gcgctgatac tgttcctcgc taccggcgtg tggtcggcat
      301 tgttcaattt cgtctcgccg cgggggattc ccttggtcgg tttctggccc accggcaagg
      361 atttagagaa gttagaaaag ccgctctcgt atcagcccac cgatccaccg cttttgagca
      421 tgctggatgc ctccgctctc taccagaaat cggatgttat cttcatcgat gcccgttccc
      481 cggcagactt cgagaaaggg catattccca gggcgatcaa tgtgccgttt gaggaagttg
      541 atttggagga attctggccg aaggtggcgg accgcgttcc caaagaaaag caggtcgtca
      601 cttactgtgg cggcgcggac tgcgaggcct cgctccactt agcccgcttc ttgaaagaga
      661 agggttactc caacctccac atcttcttcg gcgggtggaa acactggacg gagagcaaaa
      721 tgccaataga acaggggcca gtagccagta gccagtagcc agtagccagg aaagacaaag
      781 gatggaaaaa attctcggca atcggtggct gcaaatcatg ctccggctct ttctgggcgg
      841 cgtgttcatt tacgcttcag tggataagat cgctcatccc gatcaatttg cgcgcgctat
      901 ctacaattac cgaattctgc ccggcgaatt ggtcaatctc ctggcaatct ggctcccctg
      961 gctggagatt gctactggcg catttctgat cttcggcgtt tggctgcggg cgtcggcact
     1021 tttgctcacg gtacaaatag ccatgttctt catcgctgcc agttcggcct tggcgcgcgg
     1081 gttgaatttc gcctgcggct gcttcaccac cgatcccaaa gcccaatccg ccggcatcac
     1141 gcacctcttt ttagatggcg gcctcctttt gattgcactc gtcatcttat tttttgcccg
     1201 tcgacctcgg gcgggcaccg agccaatcac tgtccctgca accgccgcac cggaaccggt
     1261 ctcgggcgga tgaactcccg ctggaaaaat ctcttcggct ggtcgaagag caaagccgac
     1321 acctgggagc ggtgcaaaaa acagtttttc tacgagtacg ccgctcagta tgaggcggag
     1381 ccgctcggcg cacaggccaa atcacttaaa aagctgacca agattccctt cctgaaggga
     1441 aatctgattc atgaggcgat tgccgccgaa attcagcgcc gccgacaggg gcaggccgtt
     1501 aatcccgaac gcgccaaggc ctacgctcgt acccgaataa ccgaggtcac cgcgcagccg
     1561 aaacggtttc tctccgaagc agtcaacggt ctctctgtca ccgcgcaact gccgaaggcc
     1621 gaagaagaag tcatccagga aatcgaaacg tttttttcat ccatttggcc gcggtacgag
     1681 gagtgggaag ttctctacgt ggatggagaa gaggatttct cttccttcgt ctacggcacc
     1741 gccaaagtat ttgccgaacc ggatctggtc accaagaacc ctgacggcac ctgcctggtg
     1801 accgactgga agactggttc ggaatgggaa gagccggagg aaagcgaaga actctccgcg
     1861 cagatcctct gggtggagcg gcggtacaaa ttactcttgc cacagatcac cgcggagtac
     1921 gtctacctgc gcacggggca agtgcgcgag acccggcgta cactggaaca gatggagaag
     1981 ttcgtcaccc gcttcatccg cagcacgagc gaaatgctcc aggcggaaaa tgaatcggat
     2041 ttgccgccga ctcccagtct gaagaactgc ttggattgta aattcgccac gatctgcgag
     2101 gaggggcgcc cgttcttgcc ttccgagcct gcggtggaga caacgctatt gaattcttaa
     2161 tttttgattc gcttacccac aggatcctgc gcggcgaggg cgccgcgcct acgcgaaagg
     2221 gcacgagcta cgcgtagggc gaccgccctc ggtcgcccca acgctgctca gaggttgaaa
     2281 gaaatcgagg gagtatgtat cttctctcta tgttcgtgca ctcggagggc gagtaagcgg
     2341 aaagcggggt tggtatgcag ccgataacat ttctgttggt tggcattagt ttggtggttg
     2401 gttttggtct gggagctctg atcacctcgc tcgtaggcag agcccaaacc aagaatgcca
     2461 gggagcaatc ccagaacgcc atggagatcg tcaagcagca aattcagcag aaggacgaca
     2521 ggtttattga cctccgcaat ttgtatgaaa cggcgctgaa agatcgagcc aaggcggaaa
     2581 ccgaactcca agcagcagag aaaagtctcg cagaacagaa aacattgatt gagaaaatgg
     2641 cgggagacct taaagatgct tttgccgcca tctctcagga agcactcaaa agcaacagtg
     2701 aagaatttct caaattggca aactcgcgct ttgagaccct ggtcaccgac gccaagggcg
     2761 atttgggcaa aaggcaggag gccatcgatg ggctggtcaa gcccttgcaa gacactttga
     2821 ctcgcttcga tggagaagtt cgcaagatag aagacagccg taaagaagac tacggaagtc
     2881 tcaagcaaca tcttgagcta ctggctcaac agacgcacac tttgggcacg gcactacgga
     2941 ctccccaagt gcggggccgc tggggggaaa tcactctccg ccgcgcggtg gaactggcgg
     3001 ggatgtccga gcactgcgat ttcgtcgagc aggaatcggt ggccgcggag aacggccttt
     3061 tgcggcccga tatgatcgtt cgccttcccg gcgggaggga aattgtcatc gattctaaag
     3121 tgccgttgca agcgtttctg gacgcggcgg aggccaagaa cgatccggag cggaatgccg
     3181 ccttgctccg ccacgcggcg ctggtgcgcg agcacatgaa atagttgagc accaaggcct
     3241 actgggagca attcgagaaa acgccggact tcaccgtcat gttcattccg ggcgagtcgt
     3301 tcttcggcgc cgccttggag gtggacaagt cgctcatcga acagggtgcg cagagcaacg
     3361 tcatcctggc gacgccgacg accttgatcg cgctcttgcg caccgtggcc tacggctggc
     3421 gccaggagca aattgcgcag aatg
//
LOCUS       DBNGZA010000090         5321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2030739, whole genome shotgun sequence.
ACCESSION   DBNGZA010000090 DBNGZA010000000
VERSION     DBNGZA010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2030739"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1199
                     /locus_tag="AC16FU_02000"
     CDS             213..1199
                     /locus_tag="AC16FU_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2993183.1"
                     /translation="MQFPQEFPLRQIPWGFLKRGTLLVLIIALGGLFLCCGKKKSVTP
                     IGRGHDTIPIPPPPQRLAQNAQDEAWSPRGGFIAYSHVENVFGTEESGLYLINSDGTG
                     ERLLFPGLVYNLTWSPDGEWIAFYYQRQIFKIKLSGDSLTQLTFDQDFQHLYPRWSPD
                     GKWIAYSVPTFPPGGLWVMSSKDGSQRRQLLENGGLSFWISEDEIGYLFAVTDTTTPR
                     MWAGYMAKFNLNDSKLTILDTIGPLTSHPDYNDRTGKVIFANGKNYDYSLRIYMQDLD
                     GKNEKLVLTNHPAYFPAWLPDGGWIVYTNLDSTEGGLWITDTLGQNQRRLFR"
     gene            1388..2410
                     /gene="rfaE1"
                     /locus_tag="AC16FU_02005"
     CDS             1388..2410
                     /gene="rfaE1"
                     /locus_tag="AC16FU_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR2993184.1"
                     /translation="MTVPHPGINHLISAKRIRQLFRRFGGRKIIVLGDLMLDEYLWGS
                     VNRISPEAPVPVVEVTGSSVKLGGAANVALNLKALGDQPVVVGVIGADDKGKKLAHEL
                     ERQHLSKRGLVVDKSRPTTTKLRIIAHNQQVVRADRETAEEILDAVAERLLKVLVREL
                     HGAAGLVISDYGKGVITASILRSVIAEARGRGLFLAVDPKETHFNSYRTVTVITPNHH
                     EAAFAYGKRIRDEKTLEEVGRGLLARLDCESVLITRGERGMSLFEKNGRVTHIPTVAK
                     KVFDVTGAWDTVISSFAAAYAAGATLREAAFIANVAAGLVVEELGTAQVAKERLLADL
                     LNHSRG"
     gene            2634..3665
                     /locus_tag="AC16FU_02010"
     CDS             2634..3665
                     /locus_tag="AC16FU_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2993185.1"
                     /translation="MKALVIPKHGPPEVFIEAERPVPEIKPNEILIRTEAAGVNFADL
                     MGRMGLYPEAPKPPFVPGYEVCGVVEKIGANVAGIAVGTRVMAFSKFNGYAEYVAASA
                     AGVFPLPQNLSPTEGAAVPVNYLTAYVNLITVGNAKSGERILVYGGTGGVGNAMLQLA
                     RGLGVEIFATCGSEEKVAYLKKLGVEHAINYRTSDVLAAVRRLTAGKGVHLVLDPVGG
                     ESIRRDFHMLAPLGRIVCYGASSLAPGSRRNIFTALWQYLRFPRVNPIRLMQRNFGIL
                     GVNLNQLWNETALLRTAWQNIGEGLSSGKLRPVIAATFPFSAKGAADAHRFLHERKNI
                     GKVVFEVTR"
     gene            3781..4251
                     /gene="rfaE2"
                     /locus_tag="AC16FU_02015"
     CDS             3781..4251
                     /gene="rfaE2"
                     /locus_tag="AC16FU_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR2993186.1"
                     /translation="MNKSITLVRLTKLRRQWKRQGRKVVFTNGCFDILHRGHVEYLRK
                     AKKLGDILIVGLNSDRSVRALKGPGRPLQPQEDRAAILSALSAVDYVVIFNDLTPRKL
                     IETLKPDVLAKGADYRQSEIVGADVVKSYGGRVVRVPLFKGKSTSDLLRQVKKL"
     gene            4456..>5321
                     /locus_tag="AC16FU_02020"
     CDS             4456..>5321
                     /locus_tag="AC16FU_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HIR2993187.1"
                     /translation="MSGTRGQGAEDAPQIAASLPNCNDVPDPFFQDTNGDGIDGDTSM
                     AIFVSDAAGNDADPGTMLLPVKTIAQGISLAVANSKPHVYVAGGTYSVGTLNIADGVS
                     LYGKYGGPPSWGRSAGNTTTISGGGTAVLAPNLTLETHLEGFNISASGGFTNYAVRVI
                     GGGGNFYVRYNNISAGNGAAGFSGSPGGSGSPGSGGAPGGAFGCPGAPGPGGPGGPGC
                     GLPGGSGGATGNPGLPGAGGAPGGNPGGGPGASGFSGLPGPNGAGGSGYAINGAGLYV
                     ATSGNFGGGVPGG"
BASE COUNT         1181 a         1376 c         1587 g         1177 t
ORIGIN      
        1 gagttgaaac gacgccttta gccgaagttt tccgccacca gaagacctta gacaaacgct
       61 caattgaatt ggcggagatt ttgagtaagt agggcataca agactacctc cctcctggag
      121 aaaaaaatac taaatacata aatttgtctc ccgacaaaac ctatgcgggg gtcgtataat
      181 aagcggtaga aattcagccc ggggccgttt gtatgcagtt tccgcaagaa ttccctctta
      241 gacaaatacc ttggggcttc ctcaaaagag ggacgctttt agttttaatt attgccttag
      301 ggggcctgtt cctttgttgc gggaaaaaaa agagcgttac gcctatcggg agagggcacg
      361 atactatccc tatacccccg ccgccacaaa ggttggccca aaatgcgcaa gacgaggcct
      421 ggtccccgcg tggtggtttt attgcctatt cacatgttga aaatgttttt ggcacagaag
      481 aaagcgggct ttatctcatt aattcggacg gaactgggga gcgattgctc tttcccggtt
      541 tggtttacaa cctgacatgg agtccggacg gtgaatggat agcattttat taccagagac
      601 aaattttcaa aataaaacta agtggcgata gcctaaccca actgaccttt gatcaagatt
      661 ttcaacatct atacccacgt tggtcacctg acgggaagtg gatagcatac tctgtgccca
      721 ctttcccacc cgggggtctc tgggtaatgt catctaagga tggttcgcag agacgccaat
      781 tactcgaaaa cggcggcctt tcgttctgga tttcagagga cgagattggc tatctatttg
      841 cagttaccga caccactacc ccgcgtatgt gggcaggata catggccaag tttaacctca
      901 atgacagtaa gctaacgatt ctcgatacta ttggtccact tacttcacac cctgactata
      961 atgacagaac tggcaaagtg atctttgcca acggaaagaa ctatgactat tcattgagaa
     1021 tttacatgca agacttagat ggcaagaatg agaaactggt tctcaccaac catccggctt
     1081 acttccccgc ctggttgccc gacggcgggt ggatcgtgta cacgaacctc gattccacgg
     1141 aaggaggatt atggattacg gacaccctgg ggcaaaatca acgcaggttg tttcgctaaa
     1201 aagcagttca gttccagagt gagtgtacat tgatagtcgg aaatgggaac agctaaactc
     1261 aagaccagat gtagaactta accgtaatcg aaggggaaag cctagcctga agcgaccttc
     1321 gctccgagcg ccgctttttg tttttgtctt gacgcaattg gttgtcttcg gtaggtttcc
     1381 ttttgcaatg acggttccgc atccgggaat caatcacctg atttcagcca aacgtatccg
     1441 tcagctcttt cgccgcttcg ggggccggaa aatcatcgtg ctcggcgacc tgatgttaga
     1501 cgagtacctc tggggcagtg tcaaccgcat ttcgccggaa gcgccggtgc cggtggtgga
     1561 ggtgaccggt tccagcgtca aattgggcgg cgcggcaaat gtggccctga acctcaaggc
     1621 gctgggtgac cagcccgtgg tggtcggtgt cattggcgca gacgacaagg gcaagaaact
     1681 ggctcatgaa ctggaacgcc agcatctgag caagcgtggt ttggtggtgg ataaaagccg
     1741 tcccaccacg accaaactcc ggatcatcgc gcataatcag caggtggtgc gggcagatcg
     1801 agaaaccgcc gaagagattt tggacgcggt cgctgaacgt ctcctaaaag tgctcgttcg
     1861 ggaactgcac ggagcggcgg gattggtcat ttccgactac ggcaaggggg tcatcactgc
     1921 atccattttg cgaagcgtca ttgccgaagc cagagggcgc ggactttttc tggcagtcga
     1981 tccgaaagag acgcacttca acagttaccg caccgtgacg gtcataaccc ccaaccacca
     2041 cgaggcggct ttcgcctacg gcaaacgcat ccgggacgag aagacgctgg aggaagtggg
     2101 gcggggactt ctggcccggt tggattgcga gtcggtctta atcacgcgcg gggagcgggg
     2161 gatgtcgctc tttgagaaaa acggtcgggt gacacacatc ccgactgtag cgaagaaagt
     2221 cttcgacgtc accggcgcgt gggatacggt catctccagt tttgctgcgg cctacgccgc
     2281 cggcgcaacg ctcagagagg cggcgttcat tgcgaacgtg gcggccgggc ttgtggtgga
     2341 ggagttgggc acggcgcagg tggcgaaaga aagacttctg gcggaccttt tgaaccatag
     2401 tcggggatag cttgtagacg aggattaaat cctccgcgcc cgtttgggcg tggcactgta
     2461 agcggcgacg atcctcgtag ggaaatgacg agatgacctc accccagccc tctcctacag
     2521 cagagggagt attattccct ccccttgagg gaagggtgag ggaggggtcg attttggcca
     2581 ctattcctga tgactgaagt cgcttctacc gtatcgagaa aagaattgcc tggatgaaag
     2641 ccctcgtcat ccccaaacac ggcccgccag aagtttttat cgaagccgag cggccggtgc
     2701 ccgaaataaa gcccaatgaa attttgatac gtaccgaggc ggcgggggtg aatttcgccg
     2761 acctcatggg gcggatgggc ctctacccgg aagcgccgaa gccgccgttc gtaccgggat
     2821 atgaagtctg cggcgtcgtg gagaagattg gcgcaaatgt cgccggcatt gccgtcggaa
     2881 cgcgggtgat ggcgttttca aagttcaacg gctatgcgga gtacgtggcg gcttcggcgg
     2941 cgggcgtttt tccgcttccc caaaatttat cgccgaccga gggcgcggcg gtgccggtga
     3001 actatttgac cgcctacgtc aatttgatta cggtaggcaa cgcaaaatcc ggcgaacgta
     3061 tcctggtgta cggtggaacc ggcggcgtgg gaaatgcaat gcttcaatta gcccgaggtt
     3121 taggcgtcga aatttttgcc acctgcggtt cggaggaaaa agtggcctat ctgaaaaaat
     3181 tgggagttga gcatgcaatc aattaccgca ccagtgatgt actggctgcg gtgcggcgcc
     3241 tcaccgcagg caaaggagtt catttagttc ttgatccagt aggtggcgag agcatccgcc
     3301 gagactttca catgctggcc ccgctggggc ggatcgtctg ctacggcgcc tcgtctttgg
     3361 cgcccggcag ccggcggaat atctttactg cgctctggca gtatctgcgc tttccgcggg
     3421 tcaatccgat tcgtttgatg cagcgtaatt ttggtatttt aggggtgaat ctaaaccagt
     3481 tgtggaatga aacggcgctc ttgcgaacgg cctggcagaa tatcggggaa ggtttgagtt
     3541 cagggaagct gcgaccggtg attgcggcga cttttccctt ctcagcgaaa ggcgccgcgg
     3601 acgcgcaccg ttttttgcac gagcgaaaga atatcgggaa agtggtgttc gaggtaacgc
     3661 gatgagtccg tcctatttaa agtcctcggt agaccggctc gtctccgagc cggtcggaga
     3721 tttaagtcgg gctcagggac gagcccgact acctgatgaa atggttttac atatccggga
     3781 atgaataaat ccattaccct tgttcgcctg acgaaactcc gccgccagtg gaaacggcaa
     3841 ggcaggaagg tcgttttcac taacggctgc ttcgacatcc ttcatcgggg gcacgtcgaa
     3901 tacctgcgca aggcaaaaaa gttgggggac atcttgattg tgggcttgaa cagcgaccgc
     3961 tcagtgcgcg cgctcaaagg cccgggccga ccgctccaac cgcaggaaga ccgggcggcg
     4021 attctctcgg cgctctccgc cgtcgattac gtcgtcatct tcaacgacct caccccgcgc
     4081 aaattgatcg aaaccctcaa acccgatgtt ctggccaaag gcgccgatta ccgacagagt
     4141 gaaatcgtcg gcgctgatgt ggtcaaatcc tacggcggcc gcgtggtgcg ggtgccgctg
     4201 ttcaaaggca aaagcacgtc agatttgctc aggcaagtca aaaagttgta aagtccctgc
     4261 atgggggcct gtcccgtcgc ctgagagtgc cccaaaattt ttcttgacaa ctaaatttat
     4321 cctgatttat taccctttag cgagggtgag ggaggggact cgcggacggg agagcttatg
     4381 cgaggtcagt tacgtaggtc acttttcatt tcagcgctta gcgcggttgg ctttttcggt
     4441 gccacctcca cctgggtgag cggaactcgc ggtcagggcg cggaggatgc gccccagatt
     4501 gcggcttcgc tgccgaactg taatgacgtc cccgacccat tcttccagga taccaatggc
     4561 gacggcatcg acggcgacac cagcatggcc atttttgttt cggacgcggc cggcaatgat
     4621 gccgatcccg gcaccatgct tttgccggtg aaaactattg cccaaggaat ttctttggcg
     4681 gtggcgaaca gcaagccgca cgtttacgtg gcgggcggca cgtatagcgt agggacgctt
     4741 aatattgccg acggcgtcag cctctacggc aaatacggcg ggccgccctc ctgggggcgt
     4801 tcggccggca ataccactac catcagcggt ggcggcacgg cagttttggc tccaaattta
     4861 acgctggaga cccatctgga aggttttaac atctccgcca gtggcggctt caccaactac
     4921 gctgtgcggg tgattggcgg gggcggcaat ttttatgttc gctacaacaa catttctgcg
     4981 ggtaacggtg ctgcggggtt ttccggctct ccgggcggct ccggttcccc cggcagcggt
     5041 ggagcgcctg gcggggcatt cggctgcccc ggtgctcccg gtccgggagg gccgggaggt
     5101 ccggggtgtg gattgccagg aggcagtggc ggtgcaactg gcaatcccgg tttgccgggt
     5161 gctggcgggg ccccgggagg taacccagga ggaggacctg gggcaagcgg ttttagcgga
     5221 cttccaggac caaacggtgc cgggggctcc ggctatgcca taaacggggc cggcttgtat
     5281 gtggccacaa gcggcaactt tggcggcgga gttcccggcg g
//
LOCUS       DBNGZA010000091         7844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2773938, whole genome shotgun sequence.
ACCESSION   DBNGZA010000091 DBNGZA010000000
VERSION     DBNGZA010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2773938"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16FU_02025"
     CDS             complement(<1..204)
                     /locus_tag="AC16FU_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993188.1"
                     /translation="MLWGISPEASQAQQSTVDTLRVLDAFGLPGDTAVIVQLHLRNTV
                     AVSGLTFRVVFDTAFLTPVFVTPP"
     gene            complement(289..2007)
                     /locus_tag="AC16FU_02030"
     CDS             complement(289..2007)
                     /locus_tag="AC16FU_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993189.1"
                     /translation="MTGKIRSGRVSVLVAAVVLGCAVGAPAGEQFTNRPPRLGVPDSI
                     HVFMGDSTRFTVTANDPNGDPVTVTVLRLPPGASFTGNNPWLFRWWTAARDTGRYWVV
                     FWAKDPHGGGDVESVLVAVQTATLFWLQVLTASGYNDGDVVVPVVLGNQDSVGGMDLL
                     INYDISALTLSSVTRTGSRLADWELFSVVPNAGGNAGDIRIVGIANTNAPGVTPPLAP
                     GIGPVAFLSFHITRDVNLAGFAIPVTFAFRAPSDNVLSDPRGNLIAQSAITFQDGAVL
                     ILTPGTPLVGDINLNGAAFDIGDAIRLINFFLNPGAFPLNAQQRVNSDINGDGIPATV
                     GDLVALIRRILEGGGGQTGGFAKNAVAAAAQVTVQPLVRNDSLILSLAAPVPVSGLFL
                     EFGNVPATVGQPELRLADPDFTLASQNVGSALRLVLYSSQGRRLPAGCTEVLSIPLRG
                     ARAPEISQAACCDSSGDLLETAVGRAVTSVTPVDFFENYPNPFNPATTIAFRLPQGGR
                     VNLSIFNILGQKVKTLVDGTLSTGRHELKWDGTSDSGQPVATGVYFSRLITEQSEALR
                     KMTLLR"
     gene            complement(2456..5326)
                     /locus_tag="AC16FU_02035"
     CDS             complement(2456..5326)
                     /locus_tag="AC16FU_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993190.1"
                     /translation="MRRFSIFVIACSLLVWAGVAVAQEAGAGPFVDSLIIESKTFSNG
                     SIGFISVMVSNDSACNGISLPIVITTLSGGAFGKVDSIKKNANIDPLSATVSLAGVNG
                     TSPDSAFVLSVDVGGGSFPAGARREAYKFWVTNNSNLGQYQLDTNRLSASKTLKFTHP
                     PTQTSFPKVVKGIETIANLPPTCDGTAPISVHFGSPASQSFTGADPEGDPLTFVKISG
                     LGAVAANGDWTLGAGATVCGDVGVYQVCAAVSDPLHHNADTCCFTLTITQNPPVLACP
                     PNQSVLHGALIDVPIGASDDACPSPLNVFLASGPGGVTGSPPGPFRYRFQTSCEDVGQ
                     HVVTLKATDGKDTVQCSFNVTVTNQAPSITCPGGVIQIQANSCDNETFPTTDADGDAL
                     TYSLGNSVPPSVGTITPGGAYQYCPTPAEAGPQHAWIVATDACGAKDSCEKNFLVVVG
                     QKFCLDIEHVTAFLGTHTVPVCVNEPLNGSDPDIPNGSCVGGFNFLFAYDPTCLSFQK
                     ATMGSFLIRCQWEFFTFRFGPMGNCGNGCPSGFIRVIALADINDAAQHHPPRGACQTD
                     NAGQWLCLWFEVTRDRNFQNTCCPIRWWFWDCNDNTISDCSGNQLWTVDSVYNAEGVG
                     LDLATEFGLGVDVSNCVSSGGPNKPRSKKFICLHDGKICIPADSTIDARGDLNLNGLA
                     NEIADAVLYENYFLQGSSVFSSDPQRRQAQIAASNVNNDAFALTVADLVYLIRILTGD
                     AQPFPKVVPGDVKVEFATTRTADGYSIQGNAGVDLGGALMVFKTTGTVSDVRTVGTAS
                     DMNVKFNQEGGQLRVLIYSHERGMKIDAGQSPVLNIVTDGNLTLVDGDASSYDGYPVA
                     ISGLGKPVPSGFAVSQNVPNPFNPSTRITLSLPAASNYTLTIYNVAGQVVKSFTGHGD
                     AGDVTITWDGTDNTGAKVASGIYFYKGVAGNSSDTKKMVLMK"
     gene            complement(5566..6423)
                     /locus_tag="AC16FU_02040"
     CDS             complement(5566..6423)
                     /locus_tag="AC16FU_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2993191.1"
                     /translation="MRVRSFLLTAGAVVGVSGMAQAQLTVQVQVVDTVLWPGDQTAKI
                     RIAMTNPNLKIGGYTLTLTIDNPQGVEFIFNRVDTLPPETAYVCVNPPACTLRDTVIN
                     RPVVYITNVDTNGSRTRNFSKVDGRWQSEIFLRVVAIAGSNSNAIPFGNGTLLRLPVR
                     VNPVADTFSQRAIQVGFALDPTQTFFSDTTGNILCFTGGPSCGLTKNAGTVTIPRSLR
                     GDVNFDGVIDIVDVVRLVQYVVFGTQEPIPDLANGDVRCPPDGVVDIIDIVSLIQYVV
                     FGVPEPCPL"
     gene            complement(7095..7706)
                     /locus_tag="AC16FU_02045"
     CDS             complement(7095..7706)
                     /locus_tag="AC16FU_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993192.1"
                     /translation="MPVPQAQPGADAASTSSKKQKTKPQQRGVRPRYPLIVFGLLGAG
                     AVIFLFIYLRSTRPPIPSKAGQSGEEAALAEINRLLSANDRQTAYRELVLQGNQAMDS
                     QRFPEAILYYTHALAIDSSAVDVRVDLGACYHFVGENQKAKGEFLRALNASPQHLVAL
                     FNLGVVYHGLGQKDSARVVWQKYLRLAPNGPLVPQIQNLLSQQ"
     gene            complement(7725..7844)
                     /locus_tag="AC16FU_02050"
                     /pseudo
     CDS             complement(7725..7844)
                     /locus_tag="AC16FU_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
BASE COUNT         1603 a         2389 c         2315 g         1537 t
ORIGIN      
        1 cggcggcgtg acaaaaaccg gcgtgagaaa cgccgtatcg aacaccactc gaaaggtaag
       61 tccggagacc gccacggtat tccgcaggtg aagttgcacg atgaccgccg tgtcgcccgg
      121 aaggccgaaa gcgtccaaca cccgcagcgt gtcgacggtc gattgctgcg cctggctggc
      181 ctccggggaa atcccccaga gcaccagacc tatgagtaga acgagaagga tctttcctgc
      241 aacgacgcga ctgcgtcggc cctgccgtac tgccggttgt gtatccatct atcgcaaaag
      301 cgtcatcttg cggagtgcct cgctctgttc ggtgattagg cgggaaaagt agacgcccgt
      361 ggccaccggc tgtccgctgt cgctggtgcc gtcccacttg agttcgtggc gtccggtgga
      421 gagggtcccg tcaacgagcg tcttcacctt ctggcccagt atgttgaaga tcgagagatt
      481 cacccgaccc ccctgtggca gacgaaaggc aatcgtggtg gccgggttga acgggttcgg
      541 gtagttttca aaaaagtcga ccggcgtaac ggaagtcacc gctcgcccca ccgcggtttc
      601 caaaagatct ccggaagaat cacagcaggc cgcctgcgag atctccggcg ccctggctcc
      661 ccgcagagga atgctgagca cctctgtaca gccagcggga agccgccggc cctgggaact
      721 gtagagcacc aaccgcaggg ccgaaccgac gttctggctg gcaagcgtaa agtcggggtc
      781 agccaagcgg agttccggtt gccctaccgt ggcgggaacg ttgccgaatt ccaaaaacag
      841 tccgctcacc ggcaccgggg cggccagaga aagaatgagt gagtcgttgc gcaccagagg
      901 ttggacggtc acttgcgcgg cggcagccac cgcattcttg gcgaagccgc cagtctgtcc
      961 tcccccccct tccagaatcc gccgaatgag cgccaccaga tcgcccaccg tggccggtat
     1021 accgtcgccg ttaatgtcgg agttcacccg ctgctgcgcg ttcagcggaa acgcccccgg
     1081 attcaaaaag aagttgatca gccggatcgc atccccgatg tcaaacgcgg caccgttcaa
     1141 attgatgtcg ccgaccaggg gcgtgccggg cgtcaggatc aaaaccgcgc cgtcctggaa
     1201 ggtgattgcg ctctgcgcaa tgagattccc gcgcggatcc gacagcacgt tgtcgctggg
     1261 tgcgcggaag gcgaacgtca cgggaatggc aaaaccggcc agattgacgt cgcgcgtaat
     1321 atggaaagag agaaaagcca ccgggccgat gccgggtgcc agaggcggcg taacgcccgg
     1381 ggcgttggtg ttggcgatgc cgacaatgcg gatatccccc gcgttgcctc ccgcattcgg
     1441 gacgacggag aataactccc agtcggccag gcgcgaaccg gttcgggtga ccgatgagag
     1501 ggtcaaagcc gaaatgtcgt aattgatcaa aaggtccatc ccgcccacgg aatcctgatt
     1561 ccccagaacc accggtacca ccacgtcgcc gtcgttgtat ccgctggcgg tcaagacctg
     1621 caaccagaac aatgttgccg tctgcaccgc caccaggacg ctttcgacgt cgccgccgcc
     1681 gtgggggtcc tttgcccaga acaccaccca gtagcgcccg gtgtcgcggg ccgcagtcca
     1741 ccagcggaaa agccagggat tgttgccggt aaaactggct cccggcggca gccgcagaac
     1801 cgtcaccgtg accgggtccc cgttcgggtc gttggcggtc acggtaaacc gggtggaatc
     1861 gcccatgaaa acatgaatgc tatcgggaac ccctaaccgg ggggggcgat tggtgaattg
     1921 ttctcccgcc ggggcaccga cggcgcaccc gagaaccact gcggccacga gcacgctgac
     1981 ccgaccggag cgtatcttcc ctgtcatcgt tcgcaactgt aaaagcttat ggcgcaaatg
     2041 cgataccgat ttgccggaca aaacgatacc tgattgacct gggaaatttt tgaacagtgc
     2101 caataaaaaa gcagttcgct ccgcctttcg gtagaactgc tcacccacac tccatccgca
     2161 ccctgtcgcc aagtcacctc ggcgagcggc ggtccgtgcc ttgcgggtcg aaatcgactc
     2221 gtaccgagac ggattctcac cggcgcggca ctgctctacc gaaacactac gctttgcgtc
     2281 gctatgcata tgcgcaagaa gggacagaca acccacgcgg ctctgtccgt atgttcctta
     2341 ccaatcttgc gcgatcgact ttcggtgtac ctcccctggt ttaagttttt ttcgatagga
     2401 gagtcccccg cacgggggac tctccctatc gttggatccg tccgcctaag gcgggttact
     2461 tcatcagcac catcttcttg gtgtccgagg agttcccggc tacacctttg tagaagtaga
     2521 tgcccgaagc caccttggcg cctgtgttgt cggtgccgtc ccaggtaatg gtcacgtcac
     2581 cagcgtcacc gtggcccgtg aaactcttca ccacctggcc agccacgttg taaatggtca
     2641 acgtgtagtt gctggctgcc ggcagtgaca gagtaatccg ggtcgacggg ttgaacgggt
     2701 tcggcacgtt ctgcgacacg gcgaaaccgg atggaaccgg cttgcccagg ccgctgatcg
     2761 ccaccgggta cccgtcgtag gagctggcgt caccatccac cagggtcagg ttgccgtcgg
     2821 tcacgatgtt caacacgggg ctttgacccg cgtcgatctt catgccacgc tcgtggctgt
     2881 agatcaacac ccgcagctgg ccgccctcct gattgaactt gacgttcatg tcggaggcgg
     2941 tgcccacggt gcggacgtcg gataccgtgc cggtggtctt gaagaccata agcgcgccac
     3001 ccaggtccac gcccgcgtta ccctgaatgc tgtatccgtc ggcggtccgt gtggtggcaa
     3061 actccacctt cacgtcgccc ggaacaacct tcgggaaggg ctgcgcgtca ccggtgagga
     3121 tgcggatcaa gtagaccagg tcggccaccg tcaaggcaaa cgcatcgttg ttgacgttgg
     3181 aggccgcaat ctgcgcctgc cgcctttgcg ggtcggacga gaacacactc gagccttgca
     3241 ggaagtagtt ctcgtagagc accgcgtcgg cgatttcgtt ggccagaccg ttcaggttca
     3301 ggtcaccgcg cgcgtcgatc gtgctgtcgg ccgggatgca aatcttcccg tcgtgcaagc
     3361 agatgaactt cttgctccgc ggcttgttcg gtccgccgga agagacgcag ttggaaacgt
     3421 ctaccccgag gccgaattca gtcgccagat caaggcccac accctcggcg ttgtaaaccg
     3481 agtcgaccgt ccacagctgg ttaccgctgc agtcggagat ggtgttgtcg ttgcaatccc
     3541 agaaccacca gcgaatcggg cagcaggtgt tctggaagtt ccggtcgcgg gtcacctcaa
     3601 accagaggca cagccactga ccggcgttgt cggtctggca ggctccccgc ggcgggtggt
     3661 gctgggccgc gtcgttgatg tcggcaaggg cgatcacccg gatgaatccg gagggacagc
     3721 cgttgccgca gttccccatc ggcccaaagc ggaaggtgaa gaactcccac tgacagcgga
     3781 tcaggaagga acccatggtc gctttctgga acgacaagca ggtcgggtcg taggcgaaca
     3841 ggaagttgaa gccacccacg cagctgccgt tcgggatgtc cgggtcgctg ccattcaggg
     3901 gttcgttcac gcagaccggc acagtgtgcg tgccgaggaa agcggtcaca tgctcgatgt
     3961 ccaggcagaa cttctggccc actacgacca ggaagttctt ttcgcaactg tccttagcgc
     4021 cacaagcatc agtggcaacg atccatgcgt gctggggacc agcctcagcc ggggtcgggc
     4081 agtactggta ggctccgccc ggggtaatcg tgccgacact cggcgggaca ctattgccta
     4141 agctgtaggt caaggcatcg ccgtcggcgt cggtcgtcgg gaacgtctcg ttgtcacaag
     4201 agttggcctg gatctggatc acgccgcccg ggcaggtgat cgacggcgcc tgattggtca
     4261 ccgtcacgtt gaagctgcac tggaccgtgt ccttgccgtc ggtggctttc agggtcacta
     4321 cgtgctggcc aacatcctca caagatgtct ggaaccggta gcggaaaggc ccagggggcg
     4381 atcccgtcac tccacccggc ccggatgcca ggaacacgtt caaaggactc ggacaggcgt
     4441 cgtccgaagc cccgatcggc acgtcgatca acgcgccgtg gagcacgctc tggttcgggg
     4501 ggcaggcgag caccggcggg ttctgggtaa tggtcaaggt aaagcagcag gtgtctgcgt
     4561 tgtggtgcaa tgggtcactg accgcagcgc acacctggta aaccccgacg tcaccacaaa
     4621 cagtagcccc ggctcccaaa gtccagtcac cgttagctgc cactgctccc agcccgctga
     4681 tcttcacgaa ggtcagggga tcaccctccg ggtcggcccc ggtaaagctc tggcttgccg
     4741 ggctcccgaa atgcaccgaa atcggcgcgg tcccgtcgca ggtcggcggc aggttggcga
     4801 ttgtctcgat gccttttacc accttcggaa agctcgtctg cgtcggcggg tgggtgaact
     4861 tcagcgtctt agaggccgag agccggttgg tgtccagctg gtactgccct aagttgctgt
     4921 tgttggtcac ccaaaacttg taggcctccc gccgcgcacc ggccgggaac gaaccaccac
     4981 cgacgtccac agacaggacg aaggcggagt ccgggctggt gccgttcacg cccgctaagc
     5041 tcactgtggc gctcaacgga tcgatgttgg cgttcttttt aatggagtca accttgccga
     5101 acgccccgcc cgagagggtg gtgatcacta tgggcaggga tatcccgttg caggccgagt
     5161 cgttggaaac catcaccgag atgaacccga tgctcccgtt gctgaaggtt ttggactcga
     5221 tgatgagcga gtccacaaag gggcccgccc ccgcctcctg cgccaccgcc accccagccc
     5281 acaccagcag gctacaggca atgacgaaaa tactgaaacg tctcatcttg tatactcctt
     5341 tggttcaagg tacacccgtg cttgttgcac gtacacattg ttcacgcttc tccttcccct
     5401 ccgttaaagt ccccgttatg ggcgggactc ttaacacgct cacctccttt gcagggcttt
     5461 gcagggaagt tttacgcttg gctcacagac atcgcgttgc gtttaggtcc tcctcacctc
     5521 ctcccgagta tggtcgccat tttacctcca cggtctgcgg ggaacttaga gtgggcatgg
     5581 ctccggcacc ccgaagacca cgtactgtat gagtgacaca atgtcgatga tatccaccac
     5641 gccgtccggc ggacaacgga cgtcgccatt cgccaggtcg ggaatcggct cctgggttcc
     5701 gaacaccacg tactgcacca gccgcacgac gtccacgatg tcgatcacgc cgtcgaaatt
     5761 cacatccccc ctgagcgatc ggggaattgt aacggtcccc gcattcttgg tcagtccgca
     5821 ggaagggccg ccggtgaagc agagaatgtt gccggtggta tccgaaaaga aggtctgggt
     5881 gggatccagg gcaaaaccga cctgtatcgc ccgctgggaa aaagtatcgg cgacggggtt
     5941 cacccgcacc ggcaatcgca aaagcgtacc gttgccgaag gggatggcgt tcgaattgct
     6001 ccccgctatc gccactaccc gcaagaatat ctccgactgc cagcggccgt cgaccttgct
     6061 gaaattgcgc gtgcgggaac cattcgtgtc cacgttggtg atgtagacca cgggccggtt
     6121 gatcaccgtg tcccgcaacg tacaggccgg cgggttcaca cacacgtagg cggtttccgg
     6181 gggcagggta tcgacccggt tgaagatgaa ctccaccccc tgcgggttgt caatggttaa
     6241 cgtcaacgta tagccgccga ttttcaaatt gggattggtc atcgcaatcc tgatcttcgc
     6301 cgtctgatcg ccgggccaca acaccgtatc caccacctga acctgcaccg ttagctgggc
     6361 ctgagccatc ccgctcactc ccaccaccgc tcctgccgtg agcaggaaac ttctgactct
     6421 catccagaca cctccctggt cattcgggtt tgcctttctt tctcctcacg tagtagcaaa
     6481 cccggctatg caacctcctt ggctttgggt aaccctgccg ttggatgaaa agtccactgc
     6541 atactgttta caccgtttga taaccgaccc cgcacggctg aaggacaaaa caccctcagt
     6601 cgccggtcgt aacagacctg tgaccctcgg gaagggctaa agagcagggt atttacaacg
     6661 aactcgaatc ggctaagatt caaccctaat ataagaggcc ggtaaatcct gtcaagtaaa
     6721 aaaatcgttg ccccaccttt tttttcagta cggtaattcg accgaaacat tgccccccag
     6781 cctattgcaa ggagagtgcc acttttggga cagcgcaccg cctccgaata tcgattgcct
     6841 attctttacg gtgaattcca ccgccgccat agaggattat gacaatctag tttattctcg
     6901 atttttcttg ggtataaccg atccggtggc aacaaattgc acaaataagg cagatagttt
     6961 caatagtcaa gaggctgatt gccggtcgcg tatttttcgt ctgagggggg gcagctgaaa
     7021 ctcagacggc aaacagcccg acatccagcc tccgctcacc acaaccagag actccttgaa
     7081 atagtttcgc cgagttattg ttgagaaagg aggttttgta tttgggggac caaagggccg
     7141 ttgggcgcca aacggaggta tttctgccag acgacccggg cactgtcttt ctgtcccagt
     7201 ccgtgataga cgaccccgag gttgaagagc gccacgaggt gctgcgggga ggcgttgagc
     7261 gcgcgcaaga attccccttt ggccttttgg ttttcgccca cgaagtggta gcacgctccc
     7321 agatcaacgc gcacgtccac ggcgctcgaa tcgatggcca gagcgtgagt gtaatagaga
     7381 atggcttcgg ggaacctctg actatccatg gcctgattcc cctggagcac cagttcgcgg
     7441 taggccgtct gccgatcgtt agcggaaagc aggcggttga tttccgccag agcggcttcc
     7501 tctcctgatt gtcccgcttt tgacggaatg ggcggacggg ttgaccgcaa atagatgaaa
     7561 agaaaaatca ccgcgccggc cccgagcagt ccgaagacga tcaaaggata gcgtggacgg
     7621 acgcctcgct gctgcggctt cgttttttgt ttttttgagg aggtgctcgc tgcatccgcg
     7681 ccgggttggg cctggggtac tggcaaggga accccgcaac cggagcagac ggaggcctcc
     7741 cgggagttga gcgtttggca ccaccggcag cggaggttct ctggcagggg acgaccgcag
     7801 cggttgcaaa accggctgcc ggcagaattg gccgcctgac aggc
//
LOCUS       DBNGZA010000092         4322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2774056, whole genome shotgun sequence.
ACCESSION   DBNGZA010000092 DBNGZA010000000
VERSION     DBNGZA010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2774056"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..1256)
                     /gene="larE"
                     /locus_tag="AC16FU_02055"
     CDS             complement(420..1256)
                     /gene="larE"
                     /locus_tag="AC16FU_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR2993193.1"
                     /translation="MSAEKLQRLAEELRTLGSVLVAYSGGVDSAFLLWAACRFLGNER
                     VLAVTANSASYPPEELESARSFARLLGLAERHLVVETDEVASENYAQNSPARCYFCKE
                     ELYTKLTPVAAQYDIPFIVDGFNASDAGDFRPGKKAADLFGNVKSPLLAAGLTKPEIR
                     EIAKAHDLPFWNKPAMACLSSRVPYGQRVTPEKLAQIAQAERFLRSLGFRQFRVRHHE
                     QVARIEVEPEELSRFLDEGIRTAIADRLREIGFLYVTLDLAGYRSGSLNEALALERKG
                     QR"
     gene            complement(1313..1819)
                     /locus_tag="AC16FU_02060"
     CDS             complement(1313..1819)
                     /locus_tag="AC16FU_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR2993194.1"
                     /translation="MTNEARMIKAKINSLALDVSTNSPVVVLSPEDSEKILPIWIGHY
                     EAWAIAMELSGIASKRPLTHDLVKAVVGSLGGKIQKVEVTDLVSQTFYAKIYIERNGS
                     VLTVDARPSDSIALALKSKAPIFVNETLFQEKKEETGERPEGESPDSLRERLRRINPE
                     DFGKYSLG"
     gene            complement(1898..2359)
                     /gene="rplI"
                     /locus_tag="AC16FU_02065"
     CDS             complement(1898..2359)
                     /gene="rplI"
                     /locus_tag="AC16FU_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR2993195.1"
                     /translation="MKIILRDDVEKLGKAGEVLEVKDGYARNYLLPRNLAIPATQGNL
                     RAIDQIHRQKAGREQKRQRESLKLKEAIEKVSLTAEVLVGEEDRLFGSVTALDIAELL
                     ARQGLTIDRHKIELEEPIKALGVYSVPVRLAHDVTAEVKLWVMKKEKEAEA"
     gene            complement(2371..2610)
                     /gene="rpsR"
                     /locus_tag="AC16FU_02070"
     CDS             complement(2371..2610)
                     /gene="rpsR"
                     /locus_tag="AC16FU_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR2993196.1"
                     /translation="MAEEFEPRRIKKSCRFCARGAEPIDYKNERLLRRCVTEQGKIVP
                     RRISGCCAKHQRRVATAVKRGRHLALIPFVAEVYR"
     gene            complement(2637..2990)
                     /gene="rpsF"
                     /locus_tag="AC16FU_02075"
     CDS             complement(2637..2990)
                     /gene="rpsF"
                     /locus_tag="AC16FU_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR2993197.1"
                     /translation="MLRYETTVIFDPLLEEAALQTEIDRITNQITAAGGSVVALQRWG
                     LKRLAYSIKKKKQGYYLHVVFEGPGPLPQELERNYRLSESILRFLTVRAEESGPAQPE
                     AAGGASAERQAVEVE"
     gene            complement(3061..3645)
                     /gene="pth"
                     /locus_tag="AC16FU_02080"
     CDS             complement(3061..3645)
                     /gene="pth"
                     /locus_tag="AC16FU_02080"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR2993198.1"
                     /translation="MVKLIFGLGNPGRNYNGTRHNLGFEVIDQLAAKYKVPVAPGPGD
                     FLWGTWVRANGDVVLAKPTTFMNACGEALVQMKEFFDYTPDEVLVICDDIDLPLGKLR
                     LRPDGSDGGHKGLQSVIYHLQSEDFPRLRLGIGRPVTAAAPAPEKKEQVAEYVLDRFL
                     PEEQKVKEEIVNRAVVAVEICLESGLQTAMNQFN"
     gene            complement(3967..>4322)
                     /locus_tag="AC16FU_02085"
     CDS             complement(3967..>4322)
                     /locus_tag="AC16FU_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993199.1"
                     /translation="LQYFTDRKVINLDGLVNNEVLPYYRENKGLEYFRNRNIGWLVDR
                     AGYFVHVFGPYFGPAAESSLAVVASSQDTVYTGQYILTIRVLPEGMRPPPERVLQRFY
                     RPAPITWGPVPFLKP"
BASE COUNT          906 a         1335 c         1109 g          972 t
ORIGIN      
        1 gtcaaacgat ggtggaaggc gccaaacacc gcctctacct ctacgcagtt ttttctgctc
       61 cgaagggctg tactccttta acccataagg tggatcggta acaactgcat gtatcgaatg
      121 cggttctctc cgggctaacc atgaaaaaca atcatcgagg ataagaatat atcccgccat
      181 tcagactccc gatatttttt taattagact gacaccatgt agtttacgaa aaagaaaata
      241 ctacatctag acgtcatgtc aactcaatct tcttgagaga cttacaccta atcccggttc
      301 ccgttctctt atgattccat ggttcgccat aaacccccat ttccatttaa ttcttgaaga
      361 gcggccccaa agaacttttt atgtggtatt tccttgcaat gaatattggg taagcaggtt
      421 tacctttgac ctttccgctc aagcgccagt gcctcattca aactcccgct ccggtacccc
      481 gccagatcga gcgtgacgta caggaacccg atttctcgga gtcgatccgc tatggccgta
      541 cgaatgccct catccaaaaa acgggatagt tcctcaggtt ccacctcaat gcgggccacc
      601 tgctcgtggt gtcgcacccg aaactgccgg aagcccagcg aacggaggaa gcgctcggcc
      661 tgtgcgattt gagccagctt ttccggggtc acccgctggc cgtacggaac gcgcgacgag
      721 aggcaggcca tggccggctt gttccagaag ggcaggtcat gcgctttggc aatctcccgg
      781 atttccggct tggtgagacc ggcggccaga agcggacttt tcacattgcc gaaaaggtca
      841 gccgcctttt tgccggggcg aaaatctccc gcatcgctgg cattgaaacc gtcgacgatg
      901 aagggaatgt cgtactgcgc ggcaacgggg gtgagtttgg tgtacaactc ctctttacag
      961 aaatagcagc gtgccgggga attttgagcg tagttttcgc tggcgacctc gtcggtctca
     1021 accaccagat gccgctccgc cagaccgagt aagcgggcga agcttctggc gctctccaat
     1081 tcctcgggcg ggtaactggc cgagttggcc gttaccgcca ggaccctctc attccccaaa
     1141 aatcggcagg ccgcccagag gagaaacgca gaatcgacac cgccggagta ggccaccaga
     1201 accgacccga gggtgcgaag ttcctccgcc aggcgctgga gcttttccgc agacattggc
     1261 taaccggaaa aaggcccggg gtgtacccat ccggtgaacc ccggtggtgc catcatccca
     1321 gcgagtactt gccgaaatct tccggattga tccggcgcag ccgctcccgc agcgaatcgg
     1381 gcgactcgcc ttcggggcgc tccccggtct cctctttttt ctcctggaag agcgtctcgt
     1441 tgacgaagat cggcgctttc gacttcaaag ccagggcgat ggagtcggac ggccgggcgt
     1501 ccacggtgag cacgctgccg ttcctttcga tatagatttt cgcgtagaac gtctggctga
     1561 ccagatcggt cacttccact ttctggattt tgcctcccag ggagcccacc accgctttca
     1621 ccaggtcgtg ggtcaggggg cgtttggagg cgatgccgga gagctccatg gcgatggccc
     1681 aggcctcgta gtggccgatc cagatcggca ggattttttc ggaatcttcc ggggagagga
     1741 ccaccaccgg cgagttggtg gaaacgtcca aagccagact gttgattttg gccttgatca
     1801 tccgcgcctc gttcgtcata cgctcgtctt caccaatggc ttcgctcgct ctatcaatat
     1861 atacggcagc gatccgcgct tgcaccacga aaaatgccta cgcttcggcc tctttttctt
     1921 ttttcatcac ccagagcttc acttcggcgg tcacgtcgtg cgccagccgg accggcacgc
     1981 tgtagacgcc caaggctttt atcggctctt ccaattcaat tttgtgccgg tcgatagtga
     2041 gcccctgccg ggccagaagc tccgctatgt ccagcgccgt aaccgagccg aacagccggt
     2101 cctcttctcc gaccaagacc tccgcagtca gagaaacttt ttcaatcgcc tctttcagtt
     2161 tcagggactc tctctgtcgt ttctgctcgc gacccgcctt ctgccggtgg atttgatcaa
     2221 tcgcgcgaag attcccctga gtggccggaa tcgccaaatt acgcgggagc aggtaattcc
     2281 gcgcgtagcc atccttgact tccagcacct cgccggcctt gcccaatttt tcgacgtcgt
     2341 cgcgcaggat aattttcata ggctctgcct ctaccggtag acctcggcca caaacggaat
     2401 caaggctagg tgccgccccc gcttgacggc tgtggcgacg cgcctttggt gcttggcgca
     2461 gcagccggaa atccgccggg gaacgatttt cccctgttcg gtgacgcaac ggcgcaacag
     2521 ccgttcgttc ttgtagtcaa tcggctccgc gccccgggcg cagaagcggc agctcttttt
     2581 gattcgtcgt ggttcaaatt cctcagccat cgccactcct taagatcatt cccgtgtcat
     2641 tcgacttcta ccgcctgccg ctcggcggac gctcccccgg ccgcctccgg ctgggccgga
     2701 cccgactctt cggcgcgcac cgttaaaaaa cgcaggatgc tctcactcaa ccggtaattt
     2761 cgctccaatt cctgcggcag gggccccggc ccctcaaaaa cgacgtggag atagtacccc
     2821 tgttttttct ttttaatcga ataggccagg cgtttgagcc cccagcgctg cagggccacc
     2881 accgacccgc ctgcggcagt gatttgattg gtgatccggt cgatttcggt ttgcagcgcc
     2941 gcttcttcta agagcggatc aaaaataacc gtcgtttcgt aacgcagcac tctgggtctc
     3001 ccttcggtct ctggcggccc cccggcggat caccagaccc gcctggggag cagggtacac
     3061 ctaattaaac tgattcatcg ccgtttgtaa accgctttcc aggcaaattt ctactgcaac
     3121 cacggcccgg ttcacaatct cctctttaac cttttgttct tccggcagga accggtcgag
     3181 aacatactca gccacctgct cttttttttc cggcgcgggc gcggccgcgg tcaccggccg
     3241 cccgatcccc aggcgcaggc ggggaaaatc ctccgactgc aggtggtaga ttaccgattg
     3301 caacccctta tgcccgccgt ccgaaccatc cggacgcaaa cgcagtttgc ctaacggcaa
     3361 atcaatgtca tcgcagatga ctaaaacttc gtcgggcgta taatcgaaaa actctttcat
     3421 ctgcaccagc gcctcgccgc aggcattcat gaatgtcgtc ggcttggcca agacgacgtc
     3481 gccgtttgcc ctcacccacg taccccacaa aaaatctccc ggcccgggtg ctaccggaac
     3541 tttgtatttc gccgctaatt gatcaatcac ctcaaacccc aagttgtgcc gcgtgccgtt
     3601 gtaattgcgg cccggattcc ctaacccgaa aatcagcttg accatgcatc gtaattatat
     3661 caaaagaagc cgccggagaa ctaatcctgc ggcttgccac aggccagtat atgcgccgac
     3721 gctcttttgt caagttcccc gtccgcaaac ctgtaaaggc aaatccgcgt gaggcggatt
     3781 cgtcctctct tcccgggaac accacccgcc ggtggcgcac acccgtagtg agcgaaggcg
     3841 aatacattgt tgttaggggc gcctggcctg aatacaaaga accccgaaca aagtcggggg
     3901 accagagagc tatcctaaac ggcaaagttg acaaaaataa ccgtgactag tacagccccg
     3961 ccgcgattac ggcttgagga atggaaccgg cccccacgtg attggagctg gccggtagaa
     4021 tctctggagc accctttccg gcgggggccg catcccctca ggcagaactc gaatcgtaag
     4081 gatgtattga cccgtataaa cggtatcttg actggacgcc acgacggcca gtgagctttc
     4141 ggctgccgga ccaaaatagg ggccgaatac gtggacgaag taccccgctc tatccaccag
     4201 ccacccaata tttcggttac ggaaatattc caaccccttg ttctctctat agtatggtaa
     4261 aacctcatta ttcaccaggc cgtctaagtt tatcactttt cggtcggtga agtactgcag
     4321 gt
//
LOCUS       DBNGZA010000093         4143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2879538, whole genome shotgun sequence.
ACCESSION   DBNGZA010000093 DBNGZA010000000
VERSION     DBNGZA010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2879538"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16FU_02090"
     CDS             complement(<1..230)
                     /locus_tag="AC16FU_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993200.1"
                     /translation="MKIYHSRRFRQRRGGTENTEKKAFVLFFLTLTVVISAAISPAFE
                     DEVRMTPHFSTFSIVALDPATGQLGIAVTSKFP"
     gene            332..1615
                     /locus_tag="AC16FU_02095"
     CDS             332..1615
                     /locus_tag="AC16FU_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2993201.1"
                     /translation="MKLSQASVAFYLTLLIGSKAYSAPPLFIKDGTLFTITKGTIQKG
                     SILIKDGKIADIGENLKPPKDAVVVDATGKYVMPGIIDVHSHMGVYAWPGVDANSDGN
                     EMTNPVTAQVRAADAVNIEDPAIWRAIAGGTTTVQILPGSGNLIGGQSVVVKLKPGRS
                     LDDMLFKGAPPGLKMAMGENPKRVYGSRNQSPMTRMGNFFVMRDAFIKAQEYQRKWDD
                     YNKKKEKDKGLPPERDLKLETLSDVMQGKYLVHVHCYRQDEMQRMIDIAHQFGFKIRT
                     FEHSLEAYKVADLLAKEGIAISTWPDWYGFKIEAWYGIPGNAAICAKHGVRVNIHTDS
                     PEKVQRLYVDAAKCVKAGMPKDEALKALTLNPAWSLGIDDRVGSIEKGKDADLAIFSG
                     DPLSIYSLVEKTLIDGEVVFDRSKMTYDGKPLSQK"
     gene            1721..2218
                     /locus_tag="AC16FU_02100"
     CDS             1721..2218
                     /locus_tag="AC16FU_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR2993202.1"
                     /translation="MRIFVITVATLLTAVATLAQQPAQQGAQITIAPAPEKTISLKPA
                     DLVWVDVPNLPQGVKRAVVHGDPAKGPFEAFVKFPDGTELVPHWHTANEFVTVISGSV
                     LFGFGEKADKTKAVEYGPGSYIYIPGKTLHWAWAKGELVFLQHYTGPADRHLVGEGSE
                     KAKEK"
     gene            2303..3619
                     /locus_tag="AC16FU_02105"
     CDS             2303..3619
                     /locus_tag="AC16FU_02105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2993203.1"
                     /translation="MSKLIWKLRLLMALISSTALAAPIAITGGKVYPISGPVIEGGTV
                     VFDNGKITAVGKNVPVPPGAQVIDATGMTVIPGLIDSYTHLGAMEIDQVDVTRDIDEA
                     ADPIEPQLKIVDGLFPESEVIPVTRTNGIATVLVAPDENNVINGQSALINLDASKFVD
                     SIIVKSPVGMHVTLGEAPKARYGPKNQMPMSRMGTAALLRQTLIKAKEFLAKQEDYQK
                     KLQEYETKKNDPKAKKEDLKEPEKPGRDAVLEALVPVIKGELPVIVSAHRVDDIETAI
                     RIAEEFNLKLILNHCTNGYKIADLLAQKKIPVLVGPITTQPGSMETQGALYENAALLQ
                     KAGVKIAIQSGEVHNVRNLPYEAGIAVSYGLPWEEALKAITLTPAEILGVASRLGSLE
                     PGKDANIAIFDGDPLQPLSNCKKLFINGRDVPLTNYQTELYEKYNK"
     gene            3764..>4143
                     /locus_tag="AC16FU_02110"
     CDS             3764..>4143
                     /locus_tag="AC16FU_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR2993204.1"
                     /translation="MPELPDLEVVREFLAENVAGMTIAEFKVLRPLVLRPLVQGDVAA
                     QVTDKKILSVERRGKLLLLFLSGDLVLVIHPMLAGRLQYCDPKDKLRQNTFATFTLSN
                     GKQLRYFDDETMGMIYVVEQDKLNS"
BASE COUNT         1010 a         1174 c         1146 g          813 t
ORIGIN      
        1 ggaaattttg atgttaccgc gatgcctaat tgcccggttg ccggatcaag ggcaactatg
       61 gaaaaagtcg aaaagtgtgg tgtcatccgt acctcatcct caaaagccgg acttattgca
      121 gcgcttatta ccaccgtgag tgttagaaag aacaaaacga atgccttttt ctccgtgttc
      181 tccgtgcctc cccgcctttg gcggaatctg cgactgtggt agattttcat ttctcctcct
      241 tttgacaccc gtttttaccc gactatattt gcccgcaatt tatgcgttgc tccggccgtt
      301 gtcattcgct ttccccagta aaggaggttg cgtgaagctg tcccaagcat ccgtcgcgtt
      361 ttacttgact ttgctcatcg ggtcaaaggc gtatagcgcc ccgccgctct tcatcaagga
      421 cggcaccctg ttcaccatca ccaaagggac gatccagaaa gggtcgatcc tgatcaaaga
      481 cggaaaaatc gccgacatcg gcgaaaacct caagccgccc aaggatgcgg tggtggtcga
      541 cgccacaggc aaatatgtga tgccggggat tatcgacgtc cactcccaca tgggcgtcta
      601 cgcctggccg ggagtcgatg ccaactccga cggcaacgag atgaccaatc ccgtcaccgc
      661 gcaggtgcgg gcggcggatg cggtcaatat cgaagaccca gccatctggc gggctattgc
      721 cggcggaacg accacggtgc aaatcctgcc cggctcgggc aacctgatcg gcggtcaaag
      781 cgtggtggtc aaactcaaac cggggcgctc cttggatgat atgcttttca aaggcgcgcc
      841 gccggggctc aaaatggcca tgggggaaaa ccccaaaaga gtctacggct cgcgcaacca
      901 gtcgcccatg acccggatgg gcaacttctt cgtgatgcgg gacgctttta tcaaagcgca
      961 ggaataccag cgcaagtggg atgattacaa taaaaagaaa gagaaggaca agggcctgcc
     1021 gccggagcgg gatttgaaac tggaaacgct gtctgatgtg atgcagggga aatacctggt
     1081 gcacgtccat tgctaccggc aggacgaaat gcagcggatg atcgacatcg cccaccagtt
     1141 cggctttaag attcggacct ttgagcactc gctggaggcc tacaaagtgg ccgacctgct
     1201 ggcgaaagaa ggcattgcca tttccacctg gcccgactgg tacggcttca aaatcgaggc
     1261 ctggtacggc attccgggca acgccgccat ctgcgccaaa cacggtgtgc gggtgaacat
     1321 ccacaccgat tcgccggaaa aagtccagcg cctgtacgtc gatgccgcca agtgcgtcaa
     1381 ggccgggatg ccgaaggacg aagcgctcaa ggccctcacc ctcaatccgg cctggagtct
     1441 gggtattgat gaccgggtcg gctcgattga aaaaggcaag gacgccgact tggcgatttt
     1501 ctccggcgac ccgctctcga tatattcgct ggtggagaaa acgctgatcg acggcgaagt
     1561 ggttttcgac cgctcgaaga tgacctatga cggcaagccg ctctcgcaaa agtgacgaga
     1621 gttccctccc cttgagggga gggatcaagg gaggggtcat gaactttgac ctcaccccga
     1681 ccctctccta caggagaggg agaaccctga ggaggaaagc atgcgcatct tcgtaatcac
     1741 agtagcaact ctattgacgg ctgtcgccac cctcgcgcag cagcccgccc aacagggagc
     1801 acaaatcact attgcgccgg ctccggaaaa aaccatctcc ctgaaacccg ccgatctggt
     1861 ctgggtcgat gtgcccaacc tgccgcaagg cgtcaagcgc gccgtcgtcc acggcgaccc
     1921 ggccaagggg cctttcgagg ccttcgtgaa gtttcccgat ggcaccgaac tggtgcccca
     1981 ctggcacacg gccaacgaat tcgttaccgt catctccggc tcggtgcttt ttggttttgg
     2041 agagaaggca gacaaaacca aagccgttga gtacggcccg gggagttaca tctacattcc
     2101 cggcaagacc ctgcactggg cgtgggccaa aggagaattg gttttcctcc agcactacac
     2161 cggcccggcg gaccggcatt tggtgggtga aggaagtgaa aaagcaaagg agaaatagcg
     2221 agttatagtg gcgtcaggag ttactcctga cgctgtgtcg ggagtaactt ccgacgcgaa
     2281 caaccggaat gaagggaaaa caatgagtaa actgatctgg aagctcagat tactgatggc
     2341 actcatctca agcactgccc tcgccgcccc catcgccatc accggcggca aggtctaccc
     2401 catctccggg ccggtgattg aaggcgggac ggtggtcttc gacaacggca aaatcaccgc
     2461 cgtgggcaaa aacgttcccg ttccccccgg cgcgcaggtg attgatgcca ccggaatgac
     2521 cgtaatcccc ggcttgatcg actcctacac ccacctcggt gctatggaaa tcgaccaggt
     2581 tgacgtcacc cgcgatattg acgaagcggc tgaccccatt gagccgcagt tgaaaatcgt
     2641 tgatggcctc ttcccggaaa gcgaggtgat tccggtcacg cggacgaatg gaattgcgac
     2701 tgtcctcgtg gcgccggatg aaaacaacgt catcaacggt cagagtgcgc tcattaacct
     2761 tgacgcgagc aagttcgttg attcgattat cgtaaaatcg ccggtgggga tgcacgtcac
     2821 cttaggtgaa gcgcccaagg cgcgctacgg gccgaaaaac cagatgccga tgtcgcgcat
     2881 gggcaccgcc gcgcttttga ggcaaacgtt gattaaggcg aaagaatttt tagccaagca
     2941 ggaagattac cagaagaaat tgcaggagta tgagacgaag aagaacgacc ccaaggcaaa
     3001 aaaggaagat ttgaaagagc cggaaaaacc gggccgcgat gcggtgcttg aagcgctggt
     3061 gccggtcatc aagggcgaac tgccggtgat cgtctccgct caccgggtgg acgacatcga
     3121 aaccgccatc cgcatcgccg aagaattcaa tctaaaattg attttgaacc actgcaccaa
     3181 cggctacaag attgccgacc ttttagcaca gaagaaaatt ccggtgttag tcggccccat
     3241 caccacccag cccggctcaa tggagacgca gggcgcactg tacgaaaatg cggcgctctt
     3301 gcagaaagcg ggcgtcaaaa tcgccatcca gtcgggcgag gtgcacaacg tccgcaacct
     3361 gccctacgag gcgggcattg ccgtctccta cggtctgcca tgggaggaag cgctgaaggc
     3421 gattacgctc accccggcgg aaattctcgg cgtggccagt cgcctcggct cactggaacc
     3481 gggcaaagac gccaacatcg cgattttcga cggcgatccg ttacagcccc tctcaaattg
     3541 caaaaagctg ttcatcaacg gcagagacgt tccgctgacg aactaccaga cggaactgta
     3601 tgagaagtat aacaagtaaa tgctgaatcc tattcctttg acctcacccc ggccctctcc
     3661 tataggagag ggagcataaa ccttctcaat acagagttga atttccctcc cctcggaggg
     3721 gagggttagg gtggggtcaa aagaaaacgt ctttcaaata aacatgcccg aactccccga
     3781 cttagaagtc gtccgcgaat ttttggccga aaatgtcgcc ggcatgacca tcgccgaatt
     3841 caaagtcctg cgccctttag tccttcgtcc actggtgcag ggagacgttg ccgcgcaggt
     3901 cacggacaaa aaaatcctct ctgtggagcg gcgcggaaaa ctgcttctgc tcttcctctc
     3961 cggtgatctc gtcttagtca tacacccaat gttggcgggg cggcttcagt attgcgaccc
     4021 gaaagacaaa ctaaggcaga acaccttcgc cacttttact ctgtcaaacg gcaagcaact
     4081 ccgctacttt gacgatgaga cgatggggat gatttacgtt gtggagcaag acaagctcaa
     4141 ttc
//
LOCUS       DBNGZA010000094         3014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2666961, whole genome shotgun sequence.
ACCESSION   DBNGZA010000094 DBNGZA010000000
VERSION     DBNGZA010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2666961"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="AC16FU_02115"
     CDS             complement(<1..288)
                     /locus_tag="AC16FU_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2993205.1"
                     /translation="MPKFVSFNPGPSQVYDEIVADFKRAVEEHILSLSHRSEEFQEIF
                     SRAVDGIRTYFSLPTDYRIYFVSSATECMEIVLRGCCQYRSFHFVNGAFARK"
     gene            complement(297..1517)
                     /gene="serA"
                     /locus_tag="AC16FU_02120"
     CDS             complement(297..1517)
                     /gene="serA"
                     /locus_tag="AC16FU_02120"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR2993206.1"
                     /translation="MAKGTRPAIKVLLLEKINPGAEEQFRAQKYRVTLRAGALSEPEL
                     AGVLGPVQILGIRSKTQVTPKILQQAENLLAIGCFCIGTDQVDLRACTERGIAVFNAP
                     YSSTRSVTELVIGEIIMLLRRVTERSTAAHHGRWLKSDDSSFEVRGKTLGIFGYGHIG
                     SQVSVIAEALGMNVIYYDILDKLSLGNARSVHSLSELLRRADIVTLHVPEDKTTRHMI
                     GAREMAQMKKGSYLINTSRGSVVVLEDLYRALVDGHLRGVAIDVYPNEPSGPNARFTC
                     LLQGLPNVILTPHIAGSTQEAQADIAQKTSSRLIRVLEFGTTTGSINFPEVELPVLNQ
                     NHRIIHIHRNVPGVIRQVSAILAQHKYNIEGEYLRTTHELGYLVTDVNRKVTPEVLAE
                     LKRVRATLKVRVLF"
     gene            1638..>3014
                     /locus_tag="AC16FU_02125"
     CDS             1638..>3014
                     /locus_tag="AC16FU_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2993207.1"
                     /translation="MVGQTISHYKILEKLGEGGMGEVYLAEDTDLNRKVALKFLPQQL
                     TADPETKLRFKQEAQAAAALNHPNIITVYEVAEYQRAGYIAMEYVAGQSLRDLAAGRG
                     LPAGKALDIAIQIAEGLEKAHQAGIVHRDIKSDNILINQDGRVKILDFGLAKLKGASR
                     LIQPGSTAGTVAYMSPEQATGEEVDQRSDLFSLGVVFYELLTGQLPFPGEHQTAILYS
                     ILNEPPQPLARFAGEVPAQLQEIVEKMLAKKREERFQSAAELVVDLRRCKKELEEGKP
                     EAPKKEKKAVAVLYFENLSAEPDSDYFSAGITEDIITDLSKIESLRVASRNAVLPYKD
                     QPVDIPLLGRKLNLDAVLEGSVRKFGNRLRITAQLIDTKEGFHLWAERYDREMQDVFV
                     LQEEIAKKIALALQVALSAKDEAEIAAKYKGSLDAYSCYLKGRGYYYKYTKPDLLTAI
                     QMFHQALEL"
BASE COUNT          673 a          826 c          887 g          628 t
ORIGIN      
        1 cttccgggca aaggccccgt tcacaaagtg aaaactgcga tattggcagc atccccgcag
       61 gacaatctcc atacactcgg tcgctgagga gacgaagtag atgcgatagt cagtcggcaa
      121 ggagaagtaa gtgcgaatgc cgtcaacggc gcggctaaaa atctcctgaa attcttccga
      181 acggtgggag agcgaaagga tgtgttcttc caccgcccgc ttgaaatcgg ccacgatttc
      241 gtcgtaaacc tgcgaagggc cgggattaaa ggaaacgaac ttcggcattt gaagtcttag
      301 aacaataccc gaaccttgag ggtggcccga actcgcttaa gttctgcgag aacctctggc
      361 gttacttttc ggttgacgtc ggttacgagg taacccaact catgggtggt acgcaggtat
      421 tcgccttcga tattgtactt gtgctgtgcc agaattgcgc ttacctgtct gatcaccccg
      481 gggacgttcc gatgaatgtg gataatccgg tggttctggt tcagcacggg caattcgact
      541 tcggggaagt tgatgctgcc ggtagtcgtg ccgaattcca gaactcgtat cagccggctg
      601 gaggtcttct gggcaatgtc ggcctgcgct tcttgagtgc tcccggcgat gtggggcgtc
      661 agaatgacgt tcggcagacc ttgcaaaaga caggtgaagc gagcgttcgg cccggagggt
      721 tcgttgggat agacgtcaat cgctacaccg cgcaggtgtc cgtccaccag tgcgcgatag
      781 agatcttcca gcacgacgac gctcccgcgg ctggtgttaa tcaggtaact ccccttcttc
      841 atctgcgcca tttcccttgc gccgatcata tgccgtgtgg ttttatcctc cggcacatgc
      901 agggtgacga tgtcggccct gcggagtaat tcggagaggg aatgcactga ccgggcattg
      961 cccaaggaga gtttgtccag gatgtcgtag taaatgacgt tcatgcccaa cgcttcggcg
     1021 atgaccgaca cctgcgagcc gatgtggccg tacccgaaga tgcccagggt cttcccgcgc
     1081 acctcaaaac tggagtcgtc gcttttgagc cagcgtccat gatgggcggc ggtactcctt
     1141 tcggtcaccc gccgcaggag catgatgatt tcgccgatga ctaactcggt cacgctgcgg
     1201 gtgctggagt agggggcgtt gaagacggca atgccccgtt cggtgcaggc gcgcaggtcc
     1261 acctggtcag tgccgatgca gaagcagccg atggccagga gattttctgc ctgctgcagg
     1321 atttttggag ttacctgcgt tttcgagcgg atgcccagaa tttgcaccgg cccaagtacg
     1381 ccggccaact ccggctcgga aagcgccccc gctcgtaggg tgacgcggta cttttgcgcc
     1441 cgaaattgtt cctccgcgcc ggggttaatt ttttccagga gcaggacttt tattgctggt
     1501 cgggtacctt tggccattat cccgaccaaa ttgacacggc ggagggaatt agtcaactcc
     1561 ccggcggaga ctgcgggtaa cccagaaagg tttcttgaga tgcgtctccc tcgcccgaaa
     1621 gatatattca atttctgatg gtcggccaaa cgatttcgca ttacaaaatc ttggagaaat
     1681 tgggcgaagg ggggatgggg gaggtctacc tggccgaaga taccgacctg aaccgaaaag
     1741 ttgcgctcaa gttccttccg cagcaactca ccgccgaccc ggaaacaaaa ctgcgcttca
     1801 agcaggaagc ccaggcagca gccgcgctga atcatcccaa catcatcacc gtgtacgaag
     1861 tggccgagta tcaacgggcc gggtacatcg ccatggaata cgtcgcgggg caatcgctgc
     1921 gggatttggc ggccggcagg gggctgcccg cagggaaagc tctggacatt gccattcaaa
     1981 tcgctgaagg tttggagaag gcgcaccagg ctggcattgt ccatcgggat ataaagtccg
     2041 acaacattct gatcaatcag gacggacggg tgaaaatctt ggactttggc ctggccaaac
     2101 tcaaaggcgc ttcccggctg atccagcccg gctccacggc cgggacggtc gcctatatgt
     2161 cgccggagca ggccacgggc gaagaggttg accagcggtc ggatttgttt tcgctggggg
     2221 ttgtcttcta cgaactcctc actggccaac tccccttccc gggcgaacat caaacggcga
     2281 tcctctactc cattttgaac gagccgcccc agcccctggc ccgctttgcc ggtgaggttc
     2341 ccgcgcagtt gcaagagata gtggaaaaaa tgctggcgaa gaaaagagag gaacgttttc
     2401 agtcggcggc cgaactggtt gtcgatctcc gcagatgtaa gaaggaattg gaagaaggca
     2461 agccagaagc cccgaaaaaa gagaagaaag cagtcgccgt gctctatttt gagaatctca
     2521 gcgccgagcc ggacagcgac tacttctccg ccggcatcac cgaggatatc atcaccgatc
     2581 tgtcgaaaat cgaaagtctc cgggtggcct cgcgcaatgc cgttttgccc tacaaggatc
     2641 aaccggtcga tatcccgctt ttgggcagaa aactgaatct ggatgcggtc ctggagggga
     2701 gcgtgcggaa gttcggcaac cggctccgca tcaccgccca gctcatcgat accaaggaag
     2761 gcttccatct ctgggcggag cggtacgacc gcgaaatgca agacgtcttc gtgctccagg
     2821 aggaaatcgc caagaagatt gcgctggccc tccaggtcgc cctttccgca aaggatgagg
     2881 cggagatcgc cgcgaaatac aagggcagct tagacgccta cagctgctat ttgaaaggga
     2941 ggggttacta ttacaaatac accaagcccg atttgctgac cgccatccag atgttccacc
     3001 aggcgctgga gctc
//
LOCUS       DBNGZA010000095         8735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_546576, whole genome shotgun sequence.
ACCESSION   DBNGZA010000095 DBNGZA010000000
VERSION     DBNGZA010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_546576"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2895
                     /locus_tag="AC16FU_02130"
     CDS             <1..2895
                     /locus_tag="AC16FU_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993208.1"
                     /translation="PGQSLIDPPPHGRLYFQAHLRALANYWRAVSNGKLEIVGDVFPT
                     AELQAYQLPVTMAYYGSKGPWKDSSITAQLGHFFYDAVLLADTQEPAIDFSRYDALIL
                     FHAGADRQNDLGDLVAYTPNDLFTGFIVLDTSLSPRVDNDSVRITGGIIAPESPSQDN
                     RVVALNGVLAHEFGHVLGLPDLYNTTSRPPITQLGDFSLMDNFGQDVGVVASDGIRTV
                     TAFGLVPTYLDAWSRAFLGFLDTLAEIRNGQNLQLAAAALQQTGIEAVKVPISSREYF
                     LLENRQEDIDGITTNIIVDSVTNVVLGTGYRPPGQPRQFSDEYDILLPGSGIAVYHVD
                     EGVAYLLDPDFPQFTKFETNSVQTDPNRRMVALEEADGYVDFGGNYFTGFGTSTDLYR
                     AGNNTAFTPTSNPSTRTNFKANSHISITNISASSPFMTLDVKTDFTAPGWPVWAGKDT
                     GTVPVVIDTTAGRPLIFTRHGQYVLGWEKTGQPIFSNAFSDTVRNLNNSTSVHPLAVF
                     ARAAAPLSAGPSVADINGDGAVDVVAVGTDRKLYVWTNRDANIDGLADSLPGFPVSLV
                     DTVLLAPVCLNDSTIPFLPNARILVGGKNERFDAFSAGGTSVAVGSLGAAIEGISVDE
                     TGKIYLQLRRPEGVTLTEFPNIWLRMITSGTQATAGVWGPVTGDLNRDGVPDIVLVDQ
                     SGLVHAFDSAGVYLPGFPVRFSQPFVAEPILADFDGDGYLDIVGVTQNKIAVFNRAGV
                     MVTDFPVGPEVHDLAGYLPQPPVFLPVDNSNVGMFLASTRNGNLNAFHQTNLAPATFP
                     LSIGSQARSTIAAVERLGRTQLFLKGSDGFLYSFLLDAGSQPLATAWPQFGYNGGHTH
                     SFPSNLLPPVARPAGPLVRKESFFPYPNPAQRATKLRYYLTEDADVTFSFFDLAGNLV
                     EEKRVRGGGSRDNDFDWDCSTMAAGVYLCRMEAKSAGQKQVEFTKIAVVR"
     gene            2910..3782
                     /locus_tag="AC16FU_02135"
     CDS             2910..3782
                     /locus_tag="AC16FU_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993209.1"
                     /translation="MSHKIIVAVLGMLVVIDWFASGELAQAADPTTVVALPAMENPLG
                     VADYFAGQSLVVYQNAAVFDSDQPPLPGTKDVYAVQSKSPGKAFLFSFLLPGLGQNYS
                     ESKIKRFIFPAIEVGLWAGWFALRSHGFDKRREYQSFADTHWDRNRYYDSLRVTYNCG
                     LGYNDDTQPCYYNPQDSTRFFYFTHHLPATKNIEYYENIGKYEQFRGGWDDPPNRTAY
                     LEMRFLSNRAFDRAKALAITSLGNHLLSAIDAAVSANKFNKKADRLSQVKFQMRLAAE
                     GNEVVPHVRMAYNF"
     gene            3787..4575
                     /locus_tag="AC16FU_02140"
     CDS             3787..4575
                     /locus_tag="AC16FU_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993210.1"
                     /translation="MRRLPLFVLSVLLAPAFVRADQILLPPSLSLQLEQQIQELGVRE
                     FALAPPNAALTPAPIGAATLQGERPRSATKAVALSLLLPGAGQFYTEQGTAARAFVGT
                     EVALWTGVLAFRTYGRLKKEDYVGFATEHAGIQSTGKDDNFYKFLTFYDNRDEYNKIS
                     RIFEPSNPFYPETNQWNWQWDSRESRTRYRELRNASKTAYRRAVYLIGGAVANRLLSA
                     VHAYRAVKQYNRKLRQEKGNLEIGLSRFRFQKPLGFRLTVTKAF"
     gene            4585..5595
                     /locus_tag="AC16FU_02145"
     CDS             4585..5595
                     /locus_tag="AC16FU_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2993211.1"
                     /translation="MFSGRPQPGWGRKARAALGVGCSVFLLVGCSASVSRKTEVLQTT
                     ATIEETPGLRLETIYVIRGQIGSAELKEPTAVVPDLFGNLFIVDSGQRRILKLDPQFR
                     LLAEFRVPGEGFSLFNGLGYAAATHAGEVFVGDTWARQVWHLDGLLRPLEPVEPRYAT
                     ADSVFQGPGGVAADESGLLTVADPANSVIFRIDVFGQKRVGEPFGHPGNLSSPAGLAL
                     DRWGNLFISDAGNRRIAVYDPGGRFIRDFGGRVLDTPAGIAVDPARRVWVADRRRNSI
                     VVFDPEGGVLTEQSDFGQLGGQLSKPTGLALDLRGKVYIADTDNNRLVICQIARETAP
                     KP"
     gene            5961..>8735
                     /locus_tag="AC16FU_02150"
     CDS             5961..>8735
                     /locus_tag="AC16FU_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993212.1"
                     /translation="MGPAQAVGLAQGLNLSLSGQVSPNSRLAASISDRASPGAGFQTS
                     RRLAELDQVKVEFSSPALQATVGDFRWQAGGSAGPGWAQTLSGFKGGFTGRGWKLQAV
                     AAATKGRWQHKSFYGQPGRQGPYLLGTGAVVPNSEKIYLNGEQLFSGSDRDYVIDYGS
                     GSVTFTPRRPIGADVRIACDFETLEGDYPQHLYGGESEIQGLSGRARFSFDLLRAIDD
                     RSSGFGASLTAAEEGRWGQTGQGDGLVAKSGAQRVGFGQGDYVALDSSSGVKIYRYAG
                     PGRGDWRVSFSVVGAGKGDYLPLAGGGYQYVGYNQGTYAPIVFLPAPQGHWQISSGLA
                     VTPLPSLSVSGQWRHTSLEPDLTVDTKSTIPGNELRFGFAWGTGTAPVQVSGNFGLRE
                     KRFQALGDSTSQIREVSWGIFGSTPEGDEHTGEFVATLNTPRVQGKLTTGLLAQSGNF
                     AARRAGGSASLSLWRNSELHLKDEEIRGAQKNFSLSSVVPYLYRHASTAELVQRVGSW
                     TVGAAWQQGTAWRAGGSYSGFGGERFGVLSARVDRGRTAVAVSRRQSDVHQTVWQRFS
                     EQILATLSSGWTRRAFSGDLTYTRQLQSNHTSLPGRKSLDLALLRTAYRPSEGALAVE
                     FFHSINQVGAGNYGERFESRELFPDTPGSSDSLTSRPYALYDSTIAGEPVPEFSQALR
                     LEYHPRRNGSVVNARSVMGDLAQRLSADVSLSQTHREKGYGYGDLGLLAFWRKSNPAA
                     LLFGEKRAAGEVRLWPVGRQAHLFARGNLDRQQYRLFGSGLRERENHQYGMGIRGAFA
                     SKSTGEVEFYLTNKNEQSVDQLTLGLSGRGGRTNVWYYLSRTLDLNGEIKILREREQV
                     GGAQTVFYSAAPLVRLRPLAGLSVSAQARWVAVTTQTPAAPLSFFQAEGRPVGQSWEW
                     SVTGEWRLRTK"
BASE COUNT         1646 a         2568 c         2604 g         1917 t
ORIGIN      
        1 cccggtcagt ccctcattga cccgccgccg cacggccgac tgtacttcca ggcccacctg
       61 cgggcactgg cgaactactg gcgggcggtt tccaacggaa agttggagat cgtcggggac
      121 gtgttcccta ccgctgaact gcaggcttac cagttaccgg tgactatggc ctactacggc
      181 tctaaggggc cgtggaagga ttcttccata accgcccaat tgggccattt cttttacgac
      241 gcggtgcttc tcgctgatac gcaggaaccg gccattgatt tctcccgcta tgatgctctg
      301 atcctctttc acgccggggc tgaccggcaa aatgatctgg gcgacctcgt cgcttatacc
      361 ccgaatgatc tctttaccgg ctttatcgtt ctggacacct cgctttctcc tcgtgtcgat
      421 aacgacagtg taagaatcac cggaggcatt attgcgccgg agtcgccctc gcaggataat
      481 cgggtggtgg cgctgaacgg cgttttggcg cacgagttcg gccacgttct cggcctgccc
      541 gacctgtata acactacgtc acgtccaccc ataacccagt taggcgattt ttctctgatg
      601 gacaatttcg ggcaggacgt gggagtggtg gcctcagacg gcatccggac ggtcaccgca
      661 ttcggcttgg tgcctaccta cctcgatgcc tggtcgcggg cgtttctggg cttcctcgat
      721 actttggcag agattcgcaa cgggcagaat ctccaattgg ccgccgccgc cctgcagcag
      781 accgggatcg aggcggtcaa agttccgatc agttcgcgcg agtatttcct tctggaaaac
      841 cggcaggagg acattgacgg catcaccacg aacatcatcg tcgattcggt cacgaacgtg
      901 gtgctcggca ccggctaccg cccgccgggg cagccgcggc aattctcaga cgaatatgat
      961 atcttgctcc ccggctcagg catcgccgtt tatcacgtcg atgagggcgt ggcctacctc
     1021 cttgatccgg actttcccca gttcaccaag tttgagacga attcagtcca gaccgacccg
     1081 aaccgccgga tggtggcctt ggaagaagcc gacggctatg tcgattttgg cggcaactac
     1141 ttcaccggct tcggcacgtc caccgacctt tatcgcgcgg ggaacaatac tgccttcact
     1201 cccacgtcaa atccttccac ccgcaccaac ttcaaagcca acagccacat ttccattacc
     1261 aacatcagcg ccagcagccc ctttatgact ttagacgtga agaccgactt cacggcgccc
     1321 ggctggccgg tctgggcggg gaaggatacg ggcacagttc cggtcgtcat cgacacgacc
     1381 gcaggccgcc cgctcatttt cacccgccac ggccagtacg tgctcggatg ggagaaaacg
     1441 ggccagccaa ttttctcaaa tgctttttcc gatacggtcc ggaacctcaa taactctacc
     1501 tcagttcacc cgctggcggt ctttgcacga gcagccgctc cgctttccgc cgggccgtcg
     1561 gtggcggaca tcaacggtga cggcgcggta gatgtcgtag ccgtaggaac tgatcgtaag
     1621 ctatatgttt ggacaaatcg cgatgcgaac atagatggtt tagccgattc tctgcctggt
     1681 tttccggtgt cgttggtgga caccgtgctt ctggctccgg tttgcctgaa tgactcgact
     1741 attccgtttc tgccgaacgc acgaattctg gtgggcggaa agaacgaacg gttcgatgct
     1801 ttttccgccg gtggtacatc agtcgctgtc ggctcccttg gagctgccat cgaaggcata
     1861 agcgttgatg aaactggcaa aatctacctg cagttgaggc gccccgaggg agtaaccctc
     1921 acggaattcc ccaatatttg gttgagaatg attacgagcg gtactcaggc caccgcgggc
     1981 gtctggggac cagtgacggg cgatctcaac cgcgacggcg ttccggacat cgttctcgtt
     2041 gatcagagcg gcctagttca cgccttcgat tcggctggcg tctacttgcc gggatttccg
     2101 gtgcggtttt cacaaccatt cgttgccgaa ccgattctgg cggatttcga cggagacgga
     2161 tatctggata ttgtcggcgt cacccagaat aaaattgcgg tcttcaaccg ggccggggtg
     2221 atggtgaccg attttccggt cggccccgag gtgcacgatc tggccggtta ccttccgcag
     2281 ccgccggttt ttctgccggt ggataattct aacgtcggca tgtttttggc ctcgacccgc
     2341 aatggcaatc tcaatgcctt ccatcaaacc aatctggctc ccgcgacgtt tccgctttct
     2401 atcggctctc aggcgcgctc gactatcgcc gcggtggagc gtttaggtcg gactcaactg
     2461 ttcctcaagg gttcagacgg cttcctctac agtttcctcc tggatgcagg ctcacagccg
     2521 ctggccactg cctggcccca gttcggctac aatggcggac acacccactc attcccctcg
     2581 aaccttttgc cgccagtggc ccggccagcg gggccactgg tgcggaaaga gtcatttttc
     2641 ccttacccca acccggcaca aagggcgacg aagttgcgtt actatttgac ggaagatgcc
     2701 gacgtaacct tttccttctt tgacctggca ggtaatttgg tagaggagaa aagggtgcgc
     2761 ggcggcggca gccgggacaa cgatttcgac tgggattgca gtacgatggc cgccggcgtg
     2821 tacctctgcc ggatggaagc gaaaagcgcc ggccagaagc aggtggaatt taccaaaatc
     2881 gccgtggtgc gatgagagag gaagttctta tgtcacacaa aatcatagtg gccgtgttgg
     2941 ggatgcttgt ggtaattgac tggttcgctt ctggcgaatt agctcaagca gctgacccga
     3001 cgaccgtcgt cgcgctcccg gcgatggaga acccgcttgg ggtggcggac tactttgccg
     3061 ggcagtcgct tgtggtttac cagaatgcgg cagttttcga ttccgatcag ccgcctttgc
     3121 ccgggacgaa ggacgtctac gcggtccaga gcaagtcgcc ggggaaggcg tttctctttt
     3181 cctttctttt gcccggcctg ggacagaact actcggagtc aaaaattaag cgcttcatct
     3241 ttccggcgat agaagtcggc ttgtgggccg gctggttcgc ccttcgctca cacggttttg
     3301 ataaacgaag agagtaccaa tctttcgccg acacccactg ggatcggaat cgctactacg
     3361 actcgctcag agttacctac aattgtggct tggggtacaa tgacgatacc cagccttgtt
     3421 attataaccc gcaggactca actcggttct tctacttcac ccatcattta ccggcgacga
     3481 agaatatcga atattacgag aacatcggga agtacgagca gtttcgcggc gggtgggacg
     3541 acccgcccaa ccggacggcc tacctcgaaa tgcgcttcct ttccaaccgg gccttcgacc
     3601 gggccaaggc cctggccatc acctccctgg gcaaccacct gctttccgcc atcgatgcgg
     3661 cggtttcggc caacaagttc aacaagaaag ctgaccggct ctcgcaggtg aaattccaga
     3721 tgcgcttggc ggcagagggt aacgaggtgg ttccgcacgt gagaatggcg tacaatttct
     3781 gatcgtatga gacgcctccc ccttttcgtc ctgtcggttt tgctcgcccc ggctttcgtt
     3841 cgggctgacc aaatactctt gccgccttcg ctctcgttgc agttggagca acagattcag
     3901 gaactgggcg tgcgggagtt tgccctcgct ccgccaaacg ccgctctgac accggcacca
     3961 attggagcgg caactcttca gggagaaaga ccgcgttcag caaccaaagc agtggcgcta
     4021 tcgctccttt tgcccggcgc agggcagttc tacaccgaac agggcacggc ggcgcgggcc
     4081 ttcgtcggca ccgaagtggc cctctggacg ggggttttgg ctttccgcac ctacgggcgt
     4141 ttgaagaagg aagactacgt cggttttgcc accgagcatg ccggcatcca gtccacggga
     4201 aaagacgaca atttctacaa gttcttgacc ttctacgaca accgggatga gtacaataag
     4261 atctcgcgca ttttcgagcc gtcaaatcct ttttacccgg aaaccaacca gtggaactgg
     4321 cagtgggatt cgcgggagtc gcggaccagg taccgggaac tgcgcaacgc ctccaagacc
     4381 gcctaccgcc gggcggtcta tttgatcggc ggtgccgtcg ccaaccgcct gctctccgcg
     4441 gtgcacgcct accgggcggt gaagcaatac aaccgcaaac tccgtcagga gaagggaaat
     4501 ttagaaatcg gtttgagccg gttccgcttc cagaagccct tgggttttcg actgaccgtc
     4561 acgaaagcct tctaattact gtctatgttt tccggacgcc cacaaccggg gtgggggcgc
     4621 aaggccagag ccgcgctggg tgtggggtgt tcggttttcc ttttggtcgg ctgttcggcc
     4681 tcggtttccc ggaaaaccga ggttctccag accaccgcga ccatagaaga aactccaggt
     4741 ctccggctgg aaaccatcta cgtcatccgc ggccagattg gctccgccga actgaaagag
     4801 ccgacagccg tcgtccccga cctgttcggc aatctcttca tcgtcgattc ggggcaaagg
     4861 cgtatcctga aacttgatcc gcaatttcgc cttttggccg agttccgggt gcccggcgaa
     4921 gggttttcac ttttcaacgg tctggggtat gcggccgcca cgcacgccgg tgaggttttt
     4981 gtcggcgaca cctgggcgcg ccaggtctgg catctggatg ggttgctgcg gccgctggag
     5041 ccggtcgaac cccggtacgc gactgccgat tccgtttttc aagggccggg cggtgtggcc
     5101 gcagacgaaa gcggtctttt gaccgtggcc gacccggcca atagcgtgat tttccggatt
     5161 gacgtctttg gccagaagcg ggtgggggaa ccgttcggcc atccggggaa tctctcttcc
     5221 cctgcgggac tggcgctcga ccgctgggga aatctcttca tcagcgatgc cggcaaccgg
     5281 cggattgccg tttacgatcc gggcggacgc ttcatccgtg acttcggcgg ccgggtgctg
     5341 gatacacccg ccggcattgc cgttgatccg gcccggcggg tctgggtggc cgaccggagg
     5401 agaaacagca tcgtggtgtt tgacccggaa ggcggtgttt tgacggaaca aagcgacttc
     5461 ggccagctgg gagggcagct ctctaagccg accggcctgg cgctggatct gcgggggaaa
     5521 gtctacatcg ccgacaccga taataatcgc ctggtgatct gtcagattgc cagggagact
     5581 gcaccgaaac cgtgaaaccg tgttctttat cccggcacct ctttttttgt agtgctgttt
     5641 tttccctcag cacgtccccc tgtttttcgg cgctctatat ccctccggag aatgcgtacg
     5701 ggaatctcca ttattctctt cccgacactc ttcccgctgc aacttcagaa cttccgagtt
     5761 ccaccgcaca gtctctcaat ctttcccttc gggttctgcc catcggccaa cgctatttca
     5821 accgccaacc ccgaacgttc gtggaagaat cctcaatcgt ttccgaacga ccaccccgtc
     5881 taccctcccc aaagacacca gcggctcggc ccgctccttt tgacctctcg gtttcgggag
     5941 ccaagtcgtt tgcggtagaa ttggggccgg cgcaagcggt gggtctggcg cagggtttaa
     6001 acctctcgct ttcggggcag gtcagtccca acagccgcct ggctgcctca atttctgatc
     6061 gcgccagccc cggcgccggt tttcagacct cgcggcggct ggctgaattg gatcaggtca
     6121 aagtcgaatt cagttcgcct gccctccagg cgacggtggg cgattttcgc tggcaggcgg
     6181 gcggatcggc agggccgggc tgggctcaga cgctttcggg atttaagggc ggatttaccg
     6241 gacgcggatg gaaactgcag gcggtggcgg cggcgaccaa aggccggtgg cagcacaaga
     6301 gtttctacgg ccagccgggg aggcagggcc cgtaccttct gggcacgggc gcggtggtgc
     6361 cgaactccga aaaaatctac ctcaatggcg agcagttgtt ctccggttcg gaccgcgact
     6421 atgtcattga ctatggttcc ggttcagtca ccttcacgcc gcggcggccg attggagctg
     6481 atgtccgcat cgcctgcgat ttcgaaactc tggaaggaga ttatccccaa cacctctacg
     6541 gcggtgaaag tgaaattcag ggattgagtg gtcgagcacg gttttcgttt gatctcttgc
     6601 gcgcaattga tgaccgaagt tccggttttg gtgcgagttt gactgcggcc gaagaggggc
     6661 gctggggtca aacgggtcag ggggatggtc tggttgccaa aagcggtgcc cagcgcgtcg
     6721 gctttgggca gggtgactac gttgctttag atagttcatc cggggttaag atttaccgtt
     6781 atgcaggccc ggggcgcggc gactggagag tcagtttctc ggtggtcggt gccggcaagg
     6841 gtgactacct gcctctggcc gggggcggct accagtacgt cggctacaat cagggaacct
     6901 acgcgccgat cgttttcctg cctgcgccgc aagggcactg gcagatcagt tccggtcttg
     6961 cggtcacacc acttccatcc ctctcggtct ccgggcagtg gcggcacacc agccttgagc
     7021 cggatctgac cgtcgacacg aaaagcacga ttccgggaaa cgaattgcgt ttcggttttg
     7081 cttggggtac cggcaccgca ccggtgcagg tgagcggaaa ttttggacta cgggagaagc
     7141 ggtttcaggc actcggcgac tcaacgagtc agatccggga agtgagttgg ggtatcttcg
     7201 gcagtacacc ggagggcgat gagcatacgg gcgaatttgt ggctacccta aacacgccga
     7261 gagtacaggg aaagctgacc accggtctcc tcgctcagtc cggcaatttt gctgcgcgcc
     7321 gggcgggcgg gtccgcctcc ctctcacttt ggcgtaatag cgaactgcat ctgaaagacg
     7381 aagagattcg cggtgcgcaa aagaatttca gtttatcgtc tgtggttcca tacctttacc
     7441 gtcacgcgag caccgcggaa ctggtgcagc gggtggggtc gtggaccgtc ggtgctgcct
     7501 ggcagcaagg cacggcctgg cgggccggcg gttcctactc cggtttcggc ggggagcgct
     7561 ttggagtgct atccgcgcgg gtggaccgcg gccggacggc tgtggcggtt tcccgacggc
     7621 aaagtgacgt ccatcaaact gtctggcaaa ggttttcgga gcaaatcctg gccacgcttt
     7681 cctccggctg gacgcgacgt gccttttccg gtgatctgac ctacacacgt caattgcaat
     7741 ccaatcacac cagcctcccc ggcagaaagt cgcttgacct ggccctgcta cggacggctt
     7801 accggccgtc tgagggtgca ctggcggttg aattcttcca ctcgatcaat caggtcggtg
     7861 cggggaacta cggtgagcgt ttcgaatcaa gagagttatt tcctgacacg ccgggaagtt
     7921 ccgattcact gacatcgcgg ccctatgcgc tctacgattc gaccatagca ggggagccgg
     7981 tgcccgaatt ctcacaggcg ttgcggttgg agtaccatcc ccgccggaat gggtcggtcg
     8041 tgaacgcacg cagtgtcatg ggggacctcg cgcagcggct gtcggctgat gtttccctat
     8101 ctcagacgca ccgggaaaag ggctatgggt acggggattt gggtcttttg gccttctggc
     8161 gcaagagcaa cccggccgca ctccttttcg gggaaaagcg cgcagccggg gaggtgagac
     8221 tctggccggt gggccggcag gcgcacctgt ttgcccgtgg taatcttgac cggcagcagt
     8281 accggctctt tgggtccggg ctgcgggaac gagagaacca tcaatacggt atgggcattc
     8341 gcggcgcctt tgcgtccaag tccaccggcg aagtggagtt ctacctcaca aacaaaaatg
     8401 aacaatcagt cgatcaactg accctgggac tatcggggcg gggcggccgt acgaatgtct
     8461 ggtactacct ctcacgtacc ctcgatctca acggcgaaat caaaatttta cgcgagcggg
     8521 aacaggtcgg cggggcgcag acggtgtttt actcggcggc gccgttggtg cgcttacggc
     8581 cgctggccgg cctttcggtt tccgcgcaag cccgctgggt ggcggtgacg acgcaaacgc
     8641 cagctgctcc gctctcgttc tttcaggccg aagggcggcc ggtcggtcag agctgggagt
     8701 ggtcggtgac cggcgagtgg cggctgcgca ccaaa
//
LOCUS       DBNGZA010000096         1717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_228334, whole genome shotgun sequence.
ACCESSION   DBNGZA010000096 DBNGZA010000000
VERSION     DBNGZA010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_228334"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..892
                     /locus_tag="AC16FU_02155"
     CDS             326..892
                     /locus_tag="AC16FU_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2993213.1"
                     /translation="MRNAKGLGLAVAGAILLAAAVSGTAQEKAKAPVFKYVGADKCKL
                     CHNTQAMGKIYDKWLASPHAKAYTTLASDSAKAAGKRLGIDDPQKSEKCLKCHTVGFA
                     AHKTQAPDLAWASEGIGCEECHGAGEKYKALTVMKDKKQSLANGLIIPDEKTCLKCHN
                     NPENPFNKPFVYAEAYKKIEHKKPPKAN"
     gene            903..1343
                     /locus_tag="AC16FU_02160"
     CDS             903..1343
                     /locus_tag="AC16FU_02160"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR2993214.1"
                     /translation="MAENKSRREFLNFILGGGLATLLGAILYPILRFVMPPAKSEAFG
                     GRVTAAKVGELAPNSGKIFPLGDTPGILVQTAGGEYKAFSAVCTHLSCTVQYRPDLGE
                     IWCACHNGHYDLSGKNIAGPPPRPLTEYPVTVAGDNVVVTQRGV"
     gene            1345..>1717
                     /locus_tag="AC16FU_02165"
     CDS             1345..>1717
                     /locus_tag="AC16FU_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR2993215.1"
                     /translation="MPFGSGFYRWLGERINLQPLLHLVKKKAVPAHRHSVWYYTGGMT
                     LFFFGVQVVTGILLLLYYRPSAEAAYESVQFIMTEVKFGWLVRSIHSWSANLMIGALF
                     VHMFSTFFMKAYRKPRELTWFS"
BASE COUNT          398 a          460 c          513 g          346 t
ORIGIN      
        1 aggaatagag gatgattttt gtcttttgat aattttggcg acccggcgtc ggaaccggct
       61 aacaagttgg ttaccagtcg gttgcggcag ttgtaaaata cgagaaaagc aatgtgaagt
      121 ttttcacaaa agcacttgac gagtccgacc gtcccgcgta tactttgtta gatatgatta
      181 taatcagatg taaatgtccg gtaaataatc gcctgaccgg cgaccgaagg caggccagag
      241 atcgggttgt ttcgaacgac aggcgacgag gtaactctat caacgacgga gtaaacgttc
      301 acgcactggg cagggaggag caggcatgag aaacgcaaaa gggctgggac tggcggtggc
      361 gggagccata cttttggccg cggccgtcag cgggaccgcg caggagaagg ccaaagcacc
      421 agtattcaag tacgtcggtg ccgacaagtg caagctgtgc cacaacacac aggcgatggg
      481 gaagatctac gataagtggc tggcctcgcc ccacgccaag gcctacacaa ccctggcgtc
      541 cgactcggcc aaagcggccg gtaagcggtt ggggatcgac gatccccaga aaagcgagaa
      601 gtgcctgaag tgccacacgg tcggttttgc agcgcacaag acccaggcac cggatctggc
      661 ctgggcgtcg gaagggatcg gctgcgagga atgccacggc gcgggtgaaa agtataaagc
      721 gcttaccgtg atgaaagaca agaagcagtc actggccaac ggtctcatca ttccggacga
      781 aaagacctgc ttaaagtgtc acaacaaccc ggaaaatccg ttcaacaagc cgttcgtgta
      841 cgccgaggcg tacaaaaaga tcgagcataa gaagccgccg aaagcgaatt agtcacggag
      901 gcatggccga gaacaaatcg cgtcgcgagt ttctgaactt cattctgggt ggaggattgg
      961 ctacgttatt gggagccatc ctctacccga ttctccgctt tgtaatgccg ccggcaaagt
     1021 cggaagcgtt cggcgggagg gtcaccgcag ccaaagttgg ggaactggcg ccgaattcgg
     1081 ggaagatctt tcctctgggt gatacccctg gcattctggt gcaaaccgcg ggaggcgagt
     1141 acaaagcctt ttcggcagtc tgcacccacc tttcctgtac cgtgcaatac cggcccgatc
     1201 tgggggagat ctggtgcgcc tgccacaacg ggcattacga cctctccggt aaaaacattg
     1261 ccgggccgcc gccccgcccc ctgaccgagt atccggtaac ggtggccgga gacaacgtcg
     1321 tcgtgaccca aaggggtgtg tagcgtgcct ttcggttccg gcttttaccg gtggctgggg
     1381 gagcggatca acctccagcc acttctgcat ctggtcaaaa agaaagcggt gccggcccac
     1441 cggcattcgg tctggtacta taccggcggg atgacgctct ttttcttcgg agtgcaggtg
     1501 gtcaccggca ttctgctttt gctctactac cgaccctccg ccgaggcggc ctacgaatcg
     1561 gtgcagttca tcatgaccga agtgaagttc gggtggctgg tgcgttcgat tcactcctgg
     1621 tcggccaatc tcatgatcgg cgccctcttc gtgcacatgt tctcaacttt tttcatgaag
     1681 gcctaccgta agccgcgcga gctgacctgg ttctcgg
//
LOCUS       DBNGZA010000097         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2561407, whole genome shotgun sequence.
ACCESSION   DBNGZA010000097 DBNGZA010000000
VERSION     DBNGZA010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2561407"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="AC16FU_02170"
     CDS             complement(<1..588)
                     /locus_tag="AC16FU_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR2993216.1"
                     /translation="MPIRKNTLRAFLFGLFLAGIFFPLPARAALSRQVVHYEITAKLD
                     TFPKAVIGQETVTWINDSPDHVSSLRFHLYLNAFKNEKSTFFKELGGRRRRGKLRGDK
                     WGYIDIQKMTLADGTDLLPTLRYEHPDDNNLDDQTVCSVKLPKPVPPGGQVTLRIEFF
                     SNLPEVFARTGYKRNFYIVGQWFPKLGVYEKAGMRG"
     gene            687..1103
                     /locus_tag="AC16FU_02175"
     CDS             687..1103
                     /locus_tag="AC16FU_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993217.1"
                     /translation="MTRNKRRTAASLLLLVLLLVLWVVFNPFGRFGWCSFGFTIYNAI
                     PRPVTDIQVRADGKVRRVAKTHDLRYEDIVWLLDPKPDLLIIATGWDGVTVPRPEIGN
                     LRDCEVRILKTEEARLLFNALKRSGKQVAIHFHSTC"
     gene            complement(1174..>2007)
                     /locus_tag="AC16FU_02180"
     CDS             complement(1174..>2007)
                     /locus_tag="AC16FU_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993218.1"
                     /translation="EMEEKVLRKTIVAVFILSLVVAATALAVDQSKLEQLPAKIEHGR
                     TLTAFPLSLGYACNLSFIYPVPDGVGDTYEGMRFDVPAGKTFTLGTVDVLLYNRPDST
                     NDTVNNVVVYVWGDSPSCPPGVPDTTRLLACDTITPAEQLAPGPWPPNGVQSVASDFT
                     SKNLHFYSGQKFHITLMNLPTDPGPFWALLDDGTCTPLAKHWVEWGPGCPPCAGTAFH
                     VVPVCFGASNDLNLAIFADGTVPTLTTYGLIIFGVLLAGTLGWVVVRRRKLATAKGSL
                     A"
BASE COUNT          439 a          551 c          596 g          421 t
ORIGIN      
        1 gccgcgcatc ccggcttttt cgtatactcc caatttcggg aaccactgtc ccacaatata
       61 gaaattgcgc ttgtagccgg tgcgggcgaa gacttcgggc agattggaga aaaattcaat
      121 ccttagcgtc acctgcccgc cgggcggcac cggcttcggc agttttacgg aacagacggt
      181 ctggtcgtcc agattgttgt catccgggtg ttcgtagcgc agcgtgggca acaaatctgt
      241 gccgtcggct aaagtcattt tctgaatgtc gatgtacccc cacttgtcac cccgcaactt
      301 cccacggcgc cggcggccgc ctagttcctt gaagaaagtc gacttttcgt ttttgaatgc
      361 gttcaggtaa agatggaacc ggagactgga gacgtgatca ggcgaatcgt taatccaggt
      421 cacggtctcc tgcccgatga ccgctttcgg aaaggtatcc agtttggcgg tgatctcgta
      481 gtggaccacc tgacgggaga gggcagccct ggcgggaagc ggaaagaaaa ttccggcgag
      541 aaacagaccg aacaaaaacg ctctcagcgt gtttttacgt atcggcacgg ggacggagca
      601 tatgcgaaag acggcgattg gtcaatcgac ttcaggggtg gcttgcctcc gtcgatatgc
      661 taagcgagag gagatccgct gccgccatga cccggaataa gagaagaacc gccgcctccc
      721 tgctgttgct ggtgttactc ctcgttctct gggtggtgtt caaccccttc ggacggtttg
      781 gctggtgctc cttcgggttc acgatctaca atgccattcc ccgtccggtg accgatatcc
      841 aggtgcgcgc cgacgggaag gtgagacggg tcgccaagac gcacgactta cggtatgaag
      901 atattgtgtg gctgctcgac ccgaaacccg atctcctcat catcgccacc ggctgggatg
      961 gcgtcaccgt cccgcgcccg gaaatcggaa acctccggga ttgtgaggtg cggatcctga
     1021 aaacggaaga ggcgcggctt ctctttaacg cgctcaagcg gtcggggaag caggtggcga
     1081 tccacttcca ttcaacctgt taggctgaaa caaaaagaga aagagcggaa tgctccgctc
     1141 tttctcttaa actacttctg actgctcttt tccctacgcc aaactgcctt ttgccgtcgc
     1201 cagcttcctc cggcgaacaa cgacccatcc cagcgtgccg gctaagagca caccgaagat
     1261 gatgagacca taagtggtga gcgtcggaac cgtgccatcg gcaaagattg cgagattgag
     1321 gtcattgctg gcaccgaagc atacgggaac cacgtggaag gctgttccgg cgcagggggg
     1381 acagccaggc ccccattcga cccagtgttt tgccagaggg gtgcaggttc catcgtccag
     1441 gagcgcccag aagggaccgg gatcggttgg caaattcata agcgtaatgt ggaatttctg
     1501 gcctgagtag aagtggagat ttttggacgt gaaatcggat gcgacagact gaacaccgtt
     1561 cggtggccag ggaccggggg ccagttgctc ggcgggggta atcgtgtcgc aggccaagag
     1621 tcgggtagta tccggaacac ccggcgggca ggaagggctg tctccccaga cgtataccac
     1681 cacgttgttc acggtatcat tcgtcgagtc agggcggtta tagaggagaa cgtccaccgt
     1741 tcccaacgtg aaggttttgc cggcgggaac gtcaaagcgc atgccctcgt acgtgtctcc
     1801 gacgccatcc ggcaccgggt agatgaagct caagttgcag gcgtacccca ggctcaaggg
     1861 aaaggccgtc agggtacgac cgtgctctat tttcgctggc aactgctcaa gcttggactg
     1921 gtcgacggcc agagcagtgg cggccacgac caacgacaag atgaacactg cgacaattgt
     1981 tttgcgcaac actttttcct ccatttc
//
LOCUS       DBNGZA010000098         6609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2244533, whole genome shotgun sequence.
ACCESSION   DBNGZA010000098 DBNGZA010000000
VERSION     DBNGZA010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2244533"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3303
                     /gene="porU"
                     /locus_tag="AC16FU_02185"
     CDS             <1..3303
                     /gene="porU"
                     /locus_tag="AC16FU_02185"
                     /inference="COORDINATES: protein motif:HMM:NF033707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HIR2993219.1"
                     /translation="VDSFSRSPDWYKITVRDNAIYKIDAPLLAAAGVNLATLDPRTIR
                     IFSGDGRALSPFNVDPRPQFQELAILVTGDVDGRFDPSDLILFYGWSVDRWTKTAAGQ
                     DTFVRHPYTRENVFWFTFGGSFPTPPKRMATRDGSLRFAAPSVPTDFTELAHFEDDRI
                     FGQTSGGDVVDYFTWYWLNNQKPPPLFFNLPGVAAAAPETVAITHTNTGMNLTVNGVL
                     TTALGPGNGISRFVTSSLVDGLNQFALEFFTNPFDPRYYLNAIEMAYPRRFQAVSDFL
                     PFGFTGTGAHRFDVSGITSTAALLLDVTNGFDQVQMINVPPPQGGVFRFADSLSGTEI
                     RYAVAGSAAWRRPLRLAREVPSNLRDPASRADLLVITHDDFVAEANQYAAYRQAQSGL
                     TTKVVKVSDIYDQFAWGLFDPVAIRDFLKYTFENWTQPAPSFVLLVGDGSYDYRNNLG
                     TGAKNYIPPFIAAPAFDASTVADENYLYFGQPGILDPPPFDQVLAMVIGRWPVRTPEE
                     VQTVIAKVKAYEATPELGEWRNQITLVADDEFGEPPFGVAEAFHTQQTEGLARHVPPL
                     FSLNKIYLFEFPFDANRKKPTAEEAIISTWNRGTLIIDYIGHGNPDVWAHESVFKRGQ
                     DIPRLTNFTRLPLVYNASCSIGFFDSPFSEGMGEELVRSPLGGAIAVIAATRLAFAFQ
                     NVALNEQVFDFLLGQDSLSIGQALYLAKLLRQYQLCFPSPPCPQPNDRQYVLFCDPAL
                     HLGAPKNRVRFLPPVPDSLAALTVAQIIGEATDTSGAPLPGFSGSADVVVFDAERPRA
                     YIMAALPNSPVTYTLPGNRIYRGKAQVNGGQFNLSFVVPKDISYGSKTAKISVYIAGG
                     STDGVGLLDSLTLAGAPVAITDSLGPEIDLRFDGRPAADGDFVRADATLTGMLRDQNG
                     INITGEPGHGIVLTLDGNRQASYDLTDRFAYDPGSFQAGTVTFTLPNLASGAHTVELK
                     AWDSANNSAVRRVTIQAASEGSLAIRDFLAYPNPFRDQTTFCYQLTRDVDQMKIMIFT
                     ESGRQIKTIEGPNQLAGYHADLVWDGCDQRGDRVANGLYLCKAVAQGRVAAGTNAVTK
                     TAEEFGKVLVVR"
     gene            3343..5919
                     /locus_tag="AC16FU_02190"
     CDS             3343..5919
                     /locus_tag="AC16FU_02190"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993220.1"
                     /translation="MKRAVGMLTVAMHFVSFSYRQGQASTSQAGVLFLRIAAGARAAG
                     MGESFVALANDATATHWNPAGLGQYPLTATWLDWELPVAEPIEKMALTHNGVPEANYK
                     RFAVWAWASGQLYHWNGRQWQTSHVYRPAGDETLESIVRKYTKISDDSTVAALMEKVA
                     EANSRFTKAALRDWREKVLALLPQESADRTSLANAFDELATNWSHLTVDEGTAAQVGE
                     QVGAVTADGSLTPEEFDKLRATLAHTGRRFLLEEIRLPYNLAFTAPVTALTGEESNLW
                     VGTESGLYRFDGSAWRRFGRADSLASEKITCLAAGPNKTIWIGTDAGVLRTDGRQVTN
                     YDTTSGLPGQPIVALAVKGERLAYAATQDDLYRLDGERWSDALAYTLRVNDTWERVAA
                     GFTGRQDDRKVAAVVKALKEVNHAGDTATVAGAVVRLPYRVALESPITTLEIDKEDHL
                     WIGTAAGLKRFAEGEFTVWGYDWHAVKEGETVQSIAAATLGSTDSAKVALLVEKIKAY
                     NQLGDEPLAAGQKIGVYDNILGSPTRSIGTNGDKILVGTAFGTLMYRNEKWSRYYHNG
                     LDKVGAAAIFAVDGELFFGALNRVVVYAHARREITFMHVNWLPELATDLAYEYLSYVQ
                     HVEGWGTLGGNVTFLNLGRNVRTSETGDTLGVFDSFDAALTLSYGTRLTRSLVGGISA
                     KVIYSNLANVGAGAELGSGRGSSVALDFGLLYQTPFPRLNLGLAVTNLGPNITYIDAN
                     QSDPLPRNLAFGWAYKVFDTPYNRLTFVGEVNKELVDLNSPRSVELKEAIVNAGLEYW
                     YGSFFALRTGYIYDQLGDIKTPTFGGGLQYSSFRIDFSYIPASRDLALANTLRLSVTG
                     RF"
     gene            5945..>6609
                     /locus_tag="AC16FU_02195"
     CDS             5945..>6609
                     /locus_tag="AC16FU_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993221.1"
                     /translation="MRKRKIVKTVGPIAVLLVAGAGAGGLAFPHLLAAQTLSVQEIVR
                     PEPTDKVTAETRALFKSSVSDKNLLHLERETPFGLPKLTGRATQVDTVHVLAIRVEFQ
                     FENPDDSTTTGRGLFDRSAPGQSLIDPPPHGRLYFQAHLRALANYWRAVSNGKLEIVG
                     DVFPTAELQAYQLPVTMAYYGSTGPWKDSSITAQLGHFFYDAVLLADTQEPAIDFSRY
                     DAL"
BASE COUNT         1315 a         2008 c         1934 g         1352 t
ORIGIN      
        1 gttgattctt tttcccggtc gcccgactgg tacaaaatca ctgtgcgcga caacgcgatc
       61 tacaaaatcg atgcgccgct gctcgcggcc gcgggggtaa acctcgcaac gcttgacccg
      121 cgcaccatcc gcatcttttc gggggatggc cgtgcactct cgcccttcaa cgttgatccc
      181 cggccgcaat tccaggaatt ggctattctg gttaccggcg atgtcgatgg ccgctttgat
      241 ccgtcagatt tgattctctt ttacggttgg tcggtcgatc gctggacgaa aactgccgcc
      301 gggcaggata ctttcgtccg ccacccctac accagagaga atgtcttctg gtttaccttt
      361 ggcgggagtt tcccaacccc gcccaaacgg atggcgacgc gtgacggttc gttgcgtttt
      421 gccgctccca gcgtgccgac cgatttcact gaactggcgc actttgaaga tgaccggatc
      481 ttcgggcaaa cctccggcgg cgacgttgtc gattatttca cctggtactg gctgaacaat
      541 cagaagccgc ctcccttatt ttttaatctt cccggcgtgg ctgcggcggc accggaaacc
      601 gtcgccatca cccacaccaa taccgggatg aacctgaccg tgaacggtgt gctgacgacc
      661 gcactgggtc cgggaaatgg gatcagccgg ttcgtaacat ccagtctggt ggatggcctc
      721 aatcagtttg ccctggaatt ttttactaac ccgtttgacc cacgctacta tctcaatgct
      781 attgaaatgg cctatccccg ccgctttcaa gcggtgagcg acttcctgcc cttcggtttc
      841 accggtactg gcgctcatag gttcgacgta agcgggatca cttccactgc agcgttactt
      901 ttggatgtca ccaacggatt tgaccaggtg cagatgatta acgtgccacc tccccaaggc
      961 ggggtcttcc ggttcgcaga cagtctgtcc ggaacagaga ttcgctacgc agtggccggc
     1021 agtgcggcct ggcgccgacc tttgcgttta gcccgggaag tgccatcgaa tctgcgtgac
     1081 cccgccagcc gagcagactt gctggtgatc acgcacgacg actttgtggc cgaggcgaat
     1141 caatatgcgg cttaccggca ggcgcagtcc ggcttgacca cgaaggtcgt aaaagtgtcc
     1201 gatatttacg accaattcgc ctggggactt ttcgatccgg tggcaatccg cgattttctc
     1261 aaatacacct ttgagaattg gacccagccc gcaccgagtt ttgtgctgtt ggtcggcgac
     1321 ggcagttacg actaccgcaa taacttaggg actggggcca agaattacat tccacccttc
     1381 atagccgcgc ccgcctttga cgccagcacc gtagccgacg agaactacct ctacttcggc
     1441 cagccgggga ttttagaccc cccgccgttc gatcaggtgt tggcgatggt cattggccgc
     1501 tggccggtgc ggacgccgga ggaagtgcaa acggtcatcg cgaaagtaaa agcgtatgag
     1561 gccaccccgg agttgggaga gtggcgcaac cagatcaccc tggtggccga tgacgaattc
     1621 ggcgagccgc ctttcggcgt ggcggaagca ttccacaccc agcagactga aggtctcgca
     1681 cgtcacgtgc cgcccctttt ctctctgaac aaaatctacc tctttgagtt tcccttcgac
     1741 gcgaaccgca agaagccgac ggcggaagaa gctattatca gcacctggaa cagaggaact
     1801 ttgattatcg actacatcgg gcacggcaac ccggatgtct gggcgcacga atcggtcttc
     1861 aagcgcgggc aggatattcc gcgcttgacc aatttcaccc gtctgcctct ggtctataac
     1921 gcctcgtgca gtatcggttt tttcgactcc cctttctccg agggaatggg tgaggaactg
     1981 gtccgctcac ctctgggcgg cgccattgcc gtcatcgccg ccacccggct ggcctttgcg
     2041 ttccagaacg tcgctttgaa cgagcaggtg tttgattttc tcttaggaca ggactcgctt
     2101 tcgatcggtc aggcactcta cttagcgaag ttactgcggc agtatcagtt gtgttttcct
     2161 tcgccgccct gcccgcagcc aaatgatcgg cagtacgttt tgttctgtga cccggcactc
     2221 catttaggcg cgccgaaaaa ccgggtgcgg ttcctcccac cggtgcccga ttctctggcc
     2281 gccctgacgg tggcgcagat tataggcgaa gcgacagata cgagtggcgc cccgcttccc
     2341 ggcttttcgg gttcagccga cgtggtggtt ttcgatgcgg aaagaccgcg ggcctacatt
     2401 atggccgcct tgcccaactc cccggtgact tacacactcc cgggcaaccg gatttatcgg
     2461 ggcaaagcgc aggtgaacgg cggtcaattt aatctctcat ttgtcgtgcc gaaggacatc
     2521 agttacggct ccaaaacagc caagatttcg gtctacatag ccggcggatc aaccgacggc
     2581 gtaggcctct tggacagttt aaccctggcc ggggcgccgg tagccatcac cgacagcctg
     2641 ggaccggaaa ttgacctgcg tttcgacggg cggcccgcgg ccgacggcga tttcgtccgg
     2701 gccgacgcaa cactcaccgg catgctccgt gatcagaatg gaattaacat caccggcgag
     2761 cccggacatg gaattgtgct caccctggat ggaaatcgcc aggccagtta cgacctgacc
     2821 gaccgctttg catacgatcc gggaagtttt caagcgggga cggtcacctt caccctgccg
     2881 aatcttgcca gcggagcgca cacggttgaa ctcaaagcct gggattcggc caacaactcg
     2941 gcggttcgca gagtcaccat ccaggcggcg tccgaggggt cgctcgccat ccgggatttc
     3001 ttggcctatc cgaacccgtt ccgcgaccag accaccttct gctaccagtt gacccgcgat
     3061 gtggatcaga tgaaaattat gattttcacc gaatcgggcc gtcaaatcaa aactattgaa
     3121 ggcccgaacc agttggccgg ctaccatgcc gatctggtct gggatggttg cgaccagcgg
     3181 ggtgaccggg tggccaacgg cctgtacctc tgcaaggcgg tggcgcaggg ccgggtagcg
     3241 gcgggaacga atgcggtcac gaaaaccgcg gaagaattcg gtaaagtact cgtcgtacgt
     3301 tagcaatcga tgcttatgat gcggcggaca aaggaggagc aagtgaaacg cgcagtcggc
     3361 atgctcaccg tagcaatgca tttcgtcagt ttttcatatc gccagggaca ggcgtccacc
     3421 agtcaggccg gagtgctctt cttgcgcatt gcggcgggcg cccgggcagc cgggatgggg
     3481 gaatcgttcg tggctttggc caacgatgcc actgccaccc actggaaccc agccggtctg
     3541 gggcagtacc ccctgaccgc cacctggctg gactgggagt tgccggtggc cgagcccatt
     3601 gaaaaaatgg cgctcaccca caacggagtc ccggaagcca actacaagcg gtttgccgtc
     3661 tgggcctggg cttccggcca gctgtatcac tggaacggtc gccagtggca gaccagccac
     3721 gtctaccggc ccgccggcga tgaaaccctg gagagcatcg tccggaagta caccaaaatc
     3781 agcgatgatt cgacggtggc ggccttgatg gaaaaagtgg cggaggccaa ttcgcgtttc
     3841 accaaagcgg cgctccggga ctggcgcgag aaggtcctgg cgcttctgcc gcaggaatcg
     3901 gccgatcgga cgagtctggc caatgctttt gacgaactgg ccaccaactg gtctcacctg
     3961 accgttgacg aagggaccgc agcccaggtc ggggaacagg ttggcgccgt gacggctgac
     4021 ggatcactga ctcccgaaga attcgataag ctccgggcca ctctggccca taccggtcgg
     4081 cgttttcttc tggaagaaat tcgtttgcct tacaatctgg cttttacggc accggtgacg
     4141 gctttaaccg gtgaggaatc taatttatgg gtcggcaccg aatccggttt gtaccgtttt
     4201 gacggcagcg cctggcggcg cttcggccgg gccgacagtc tggccagcga aaaaattacc
     4261 tgcttggcgg ccggtccgaa caagacgatc tggattggca ccgacgcggg cgtcttgcgc
     4321 accgatggcc gccaggtgac caattacgac accacttccg gcctgcccgg tcagccgatt
     4381 gtggctctgg cagtcaaggg tgaacggctg gcctacgccg ccacccaaga tgacctgtac
     4441 cggctcgacg gagagcgctg gtccgatgcc cttgcctaca ccttgcgggt aaacgatacc
     4501 tgggagcggg tggcggctgg attcaccggg cgtcaggacg accggaaggt ggcggcggtg
     4561 gtcaaggcgc tgaaggaagt caaccacgcc ggcgataccg ccaccgtggc gggggccgtg
     4621 gtgcggctgc cctatcgggt ggctttggaa agtccaatca cgacactgga aattgacaag
     4681 gaagaccatc tctggatcgg gaccgcggcc ggccttaagc gctttgccga aggggaattc
     4741 accgtctggg gctacgactg gcatgcggtc aaagagggtg aaaccgtgca atcgatagcc
     4801 gccgcgactc tcggctctac cgactcagcc aaggtggccc ttttggtgga gaagatcaaa
     4861 gcctacaacc agctgggtga cgaaccgctg gccgcgggcc agaaaatcgg cgtgtacgac
     4921 aacatcctgg gcagccccac ccgctccatc ggcaccaacg gcgacaaaat tctggtgggc
     4981 accgccttcg ggactttaat gtacaggaac gaaaaatgga gccgctacta ccacaacggg
     5041 ctggacaaag ttggtgcggc ggcaatcttt gccgtcgatg gcgaactgtt cttcggcgcc
     5101 ctaaaccggg tggtggtgta cgcgcacgcc cggcgggaaa ttaccttcat gcacgtcaac
     5161 tggctgccgg aactggccac cgacctggcc tacgaatacc tctcctacgt ccagcacgtc
     5221 gaaggctggg gcactctggg cggcaacgtc acctttctca acttaggccg caacgtgcgg
     5281 acgagcgaga ccggcgatac gctgggggtg ttcgacagct tcgatgccgc tttaaccctc
     5341 tcctacggca cccggctgac gcggtctctg gtgggcggca tttcggccaa ggtcatttac
     5401 agcaatctgg ccaacgtggg cgccggtgcg gaactgggct ccggccgcgg ctcgtcggta
     5461 gcgctcgatt tcgggcttct ctaccagacc ccctttcccc ggctcaactt aggcttggcg
     5521 gtcaccaact taggcccgaa catcacctac atcgacgcca accagtccga cccactgccg
     5581 cggaacctgg cgttcgggtg ggcctacaag gtcttcgaca cgccgtacaa ccggctgacc
     5641 ttcgtgggag aggtcaataa ggaactggtt gacctgaact ccccgcgctc ggttgagctc
     5701 aaagaggcga ttgtcaacgc cgggctggag tactggtacg gctcgttctt cgccctgcgc
     5761 acgggctaca tctacgacca actgggcgac atcaaaacgc ccaccttcgg cggcgggttg
     5821 cagtattcga gtttccggat cgacttctcc tacatcccgg cttcccgcga cctggccctc
     5881 gccaacaccc tgcggctctc ggtgaccgga aggttctaag tggccgtagt gaggccgttg
     5941 gaatatgaga aagcggaaga tagtaaagac cgttggcccg atagcagtac tacttgttgc
     6001 cggtgcgggc gcagggggtc tggctttccc ccacctgctg gcggcccaaa ctctttccgt
     6061 ccaggaaata gtgcgaccag agccaaccga taaagtcact gcggaaaccc gggcgctttt
     6121 caaaagtagc gtgtccgaca aaaacctcct gcatctggag cgcgaaacgc ctttcggcct
     6181 gcccaaactg accgggcgcg ccactcaggt ggacacggtg cacgtgctgg ccatccgcgt
     6241 ggagtttcaa tttgaaaacc cggacgattc cactacaacg ggccggggcc tttttgaccg
     6301 cagcgctccc ggtcagtccc tcattgaccc gccgccgcac ggccgactgt acttccaggc
     6361 ccacctgcgg gcactggcga actactggcg ggcggtttcc aacggaaagt tggaaatcgt
     6421 cggggacgtg ttccctaccg ctgaactgca ggcttaccag ttaccggtga cgatggccta
     6481 ctacggctct acggggccgt ggaaggattc ttccataacc gcccaattgg gccatttctt
     6541 ttacgacgcg gtgcttctcg ctgatacgca ggaaccggcc attgatttct cccgctatga
     6601 tgctctgat
//
LOCUS       DBNGZA010000099         1256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1184192, whole genome shotgun sequence.
ACCESSION   DBNGZA010000099 DBNGZA010000000
VERSION     DBNGZA010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1184192"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..603)
                     /locus_tag="AC16FU_02200"
     CDS             complement(223..603)
                     /locus_tag="AC16FU_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2993222.1"
                     /translation="MPRVLTTRKVLIVEDNQQMSRLLSEMLEVFDVESLCAASGGAAL
                     KMLEEQQFGLVITDLRMPEMSGVELLRAVKEKHPEVPVVVISGFATKKAESEIVAADA
                     DGFLQKPFRMEDIGRLLNRILPVG"
     gene            complement(728..>1256)
                     /locus_tag="AC16FU_02205"
     CDS             complement(728..>1256)
                     /locus_tag="AC16FU_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR2993223.1"
                     /translation="STLFNHLTRAEVIVEDRLFSTLDSTSRLVKWPLGPKMILTDTVG
                     FIRKLPTHLVASFRSTLDQVCQADLLLHVVDLASPHFRHQIAEVEKVLVAIEAQKIKR
                     FLVFNKIDALPNGVPLADLAGLNGEQFFISAEARLGLATLVETIRVQALSHTPVYTNS
                     AYPDGDSADGETKNA"
BASE COUNT          294 a          333 c          347 g          282 t
ORIGIN      
        1 tgatgcccgg ggcgtcacta accccgactc tttggcaaaa tgtccggatt gcacccgatt
       61 caatttcgat cctgaatctg aaaatcactt tacaacgtaa tcctaaaagt acccatgatt
      121 ggcacgagca aattgtcggg aaggacactt atcaacaaaa gtaatttagc tccggctaat
      181 tgctattacc aatgttctat ttgtgattta actgggggcg ctctacccca ccggcagaat
      241 gcggttcaaa agccggccaa tgtcttccat gcggaagggc ttctgtaaaa aaccgtcggc
      301 gtcggcggcc acgatctcgc tttccgcttt cttggtggca aaaccggaga tcaccaccac
      361 cggcacctcg gggtgtttct ccttgaccgc gcgcaggagt tcaacgccgg acatttccgg
      421 catgcgcagg tcggtaatca ccagcccgaa ctgctgttct tccagcattt tcagggcggc
      481 tccgcccgag gcggcgcaga gactttcgac gtcgaagact tccagcattt ccgacagaag
      541 acgggacatc tgctggttat cctcaactat caagactttg cgggtggtca ggactcgcgg
      601 catgtcatag taattgtcgg aagatagagg ggcaaaattt acttttttta gcgagaaatg
      661 cttctgaaat gtagtgggga cgcagtcggt gagactcgta ttgagcgaaa cgctgatcaa
      721 ctattgatca cgcgtttttc gtctcaccgt cagccgaatc gccgtcggga taggcgctat
      781 ttgtgtaaac gggagtatga cttagcgcct gcacacggat cgtttccacc agagtggcca
      841 gacccagccg cgcttcggca gagatgaaga actgctcgcc gttcaacccc gccaaatcgg
      901 ccagagggac gccattcggg agcgcgtcaa tcttgttgaa caccaggaac ctcttaatct
      961 tttgcgcttc aatcgctacc agaacctttt ccacctcggc gatctgatgc cggaaatggg
     1021 ggctggctaa atcgaccacg tggaggagca aatccgcctg gcagacctgg tctaaagtgg
     1081 agcggaaaga ggccaccaag tgggtgggca gtttgcgaat gaaacccacg gtgtcggtga
     1141 ggatcatctt cggccccaga ggccacttga ccagccggga ggtggagtcc agcgtggaaa
     1201 agaggcggtc ttccacgatg acttcggccc gggtcaggtg gttgaagagg gtcgat
//
LOCUS       DBNGZA010000100         2207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1396976, whole genome shotgun sequence.
ACCESSION   DBNGZA010000100 DBNGZA010000000
VERSION     DBNGZA010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1396976"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="AC16FU_02210"
     CDS             complement(<1..602)
                     /locus_tag="AC16FU_02210"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2993224.1"
                     /translation="MRFLTAGESHGQALAGILEGMPAGLAVDISFLNAELARRQKGYG
                     RGGRMQIEKDTLKILSGVRHLKTLGSPIAFLVENQDWENWKEVMAVEDNPDSAAVANP
                     RGAPRTAPRPGHADLAGAIKYGQKDIRNVLERASARETAARVGVGAFAKLLLREFGID
                     FASHVVEIGGVAVPDGRPAFAEIQSRAPKSELHCIDPATE"
     gene            775..852
                     /locus_tag="AC16FU_02215"
     tRNA            775..852
                     /locus_tag="AC16FU_02215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Glu,seq:ttc)
     gene            complement(986..1468)
                     /locus_tag="AC16FU_02220"
     CDS             complement(986..1468)
                     /locus_tag="AC16FU_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993225.1"
                     /translation="MTARSFDSVPYEEPDEAGLHRSHRSFQTRARQELARAERYAAYL
                     SLVTLDLSCLLRSRAPDQKNEEMNRLFDELYDLVRTRVRTSDVVSGVENARLGLLLIE
                     TAKSGAQTLAARLAEQVRAYLASHVPLPSDWQVPVEIDSFPDVHSGREQFLSRLGTFQ
                     "
     gene            complement(1796..>2207)
                     /locus_tag="AC16FU_02225"
     CDS             complement(1796..>2207)
                     /locus_tag="AC16FU_02225"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR2993226.1"
                     /translation="DHFLKKYNTQYHKEVASLSARAMTLMQQFPWPGNVRQLENVIKQ
                     VVVRGDEQIIGDLLRDRGKAVPEAALPLPSGPNTYSLKKRVEALVTQEEKRLIAEVLH
                     KTNWNRRKAADLLAISYRSLLYKIKEYRINEVAE"
BASE COUNT          458 a          686 c          600 g          463 t
ORIGIN      
        1 tcttccgtcg ccggatcgat gcagtgtaac tcggacttgg gtgccctgga ttggatttca
       61 gcgaacgctg gccgtccgtc cggcaccgca acgccgccga tttccaccac gtggctggcg
      121 aaatcgatgc cgaattcccg gaggaggagt ttggcaaagg caccaactcc cacacgcgcc
      181 gccgtctcgc gggccgatgc tcgctccagc acgttgcgaa tgtctttctg gccgtacttg
      241 atcgctccgg ccagatcggc atgccccgga cggggagcgg tgcgcggagc accgcgcggg
      301 ttagcgactg cggctgaatc cggattgtct tccaccgcca taacctcttt ccaattttcc
      361 cagtcttggt tctcgaccag aaacgcgatc gggctgccca gagttttcaa gtgacggacg
      421 ccggagagaa tcttgagggt atccttttcg atttgcatcc gtccgccccg accgtagccc
      481 ttctgccgcc gcgccagttc agcattaagg aaagaaatat cgactgccag gcccgccggc
      541 atcccctcga ggatgcccgc taaggcctgc ccgtgtgatt ctcctgccgt caaaaagcgc
      601 accatcgtct ttaccccgaa caacccaaat gagtttccgg aagtgaaaga gaaaatacga
      661 cggtggcgcg acaattccaa attacgttcg ggggaagatc aacggcccgt cttgacggaa
      721 actgcgcggc caacgtatct tcccgcagcc gcccaaggcg ggcaattacc caccgtcccc
      781 atcgtctagg ccggtctagg acaccgccct ttcacggcgg taacacgagt tcaaatctcg
      841 ttggggacac cacaaaaaac agggatcagc gatcagggat cggtgaaaaa gacggggaaa
      901 aattacaccg cttcccggcg aatgtagttc aggaagaaat gggtagccgg ccgcactcaa
      961 cgaccggctg acgtactcac acaacttact ggaaagtccc cagccgggag aggaactgct
     1021 cccggcccga gtgcacgtcg ggaaacgagt caatctccac cggcacctgc cagtcggagg
     1081 gcagggggac gtgactggcc agataggcgc ggacctgttc cgccaggcgg gccgccaagg
     1141 tctgcgcacc gcttttggcg gtttcaatca aaagcaaacc gagacgggcg ttctccaccc
     1201 ccgagaccac gtcgctcgtc cgcacccggg tacgaaccag gtcgtataac tcgtcaaaca
     1261 gccggttcat ttcctcattt ttttgatccg gcgcgcgcga gcgcaaaaga caacttaaat
     1321 cgagggtgac cagagaaagg taggccgcgt accgttcggc ccgggccagc tcctgcctgg
     1381 cgcgcgtctg gaaagatcgg tgggacctgt gcagtcccgc ctcgtcaggt tcttcgtacg
     1441 gtaccgagtc gaagcttctg gcggtcatgc tgctcccctt ccctgggttg acagtgatag
     1501 agaatttctc tcccaccgct tatcctaccg aaatcttttg ggacacccac cctcaccctt
     1561 tgatccgcat ccaggctctg ctgcgacttt tttacagcaa actcaatgcc acagtaaaag
     1621 ttacccccgg tcaaacgctc cgcggttaaa tcggtccggg caaaagcgct cctgaccttt
     1681 gccccaacga ggtagcggaa caccgtaact tttctttctg tcccactccg cgattgccac
     1741 tctgcacctg ctcgaccagg agttgcaaaa ccgttcagtt tttctacatc cccgactatt
     1801 cggccacctc gttgatgcgg tactctttta ttttgtacaa gagcgagcgg tagctgatgg
     1861 ccaaaagatc ggcggctttc cggcggttcc aattcgtctt gtgtagcact tcggcaatca
     1921 aacgtttttc ttcctgcgtc accagagctt caactctctt tttgagcgag taagtgttcg
     1981 gaccggaagg aagggggagg gcagcctctg gcaccgcctt gccgcggtcg cgcaacaaat
     2041 ctccgataat ttgttcgtcg ccgcgcacca ccacctgctt gatgacgttt tccagctgcc
     2101 ggacgttgcc cggccagggg aactgctgca tcagggtcat ggcccgggcg gaaagcgacg
     2161 ccacctcttt gtggtactgg gtgttgtact tcttcaggaa atggtcg
//
LOCUS       DBNGZA010000101         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1291995, whole genome shotgun sequence.
ACCESSION   DBNGZA010000101 DBNGZA010000000
VERSION     DBNGZA010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1291995"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="AC16FU_02230"
     CDS             complement(<1..643)
                     /locus_tag="AC16FU_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993227.1"
                     /translation="MVMKQTGKWKDHLLKSSLPLQQLVSEKLEKHELYVTGEYTYSRS
                     NERDVETEFSVDLRAFNLLKRRKRHWGNLNVLIECKYNHPGVRWIFSQHPKESDVTAG
                     CISCLEDLCTERLENKHPLYKIDNELRFCTHGVELYEQGVDPNAISHGLSQLRYALPN
                     LVADECRMQVSILNEEDLSICFICPILVTTASLYILKFGLSLQAFQCASSLNDV"
     gene            1507..3342
                     /locus_tag="AC16FU_02235"
     CDS             1507..3342
                     /locus_tag="AC16FU_02235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2993228.1"
                     /translation="MTAPERKALPPAGGDEPTLVFARHEPLPAWLLPLRLLIFVLLFG
                     IVLALLRQPPQLITPLLGYGFCTLVFLGIVTLHRRRSMPMVLRLVLAVQIVWEVFLEA
                     GIVHQSGNLSQASPYNALFLLTIVSASLVYRLIGTLAIATAASLFYSFAIWGTNPATL
                     PWPWDFSLRQILAATGDDSFAVIFVHICVFYITAFISGYLAERLQHKGEELAETSEQL
                     QKARLDTDDILHHLYSGLLTIDRAGKVIYFNQAAQEILGYRSPEIQGRDFRRVFGHRM
                     PDLVERLQQVLESGRMEPRFELVIVSRSGRQCPVGFTLSPLKDPQGNPRGVIGVFQDL
                     TEIKLLEDRIRTSDRLAAVGELSAGIAHEIRNPLAAIAGSVEVLRKELQLTGDEKRLL
                     DLVIRESERLNQILSEFLHFARIRPTLLTAVDLASVTRETIEALKRQPLFSGGSELGP
                     RRKIEMKLQSDPALAPVWGEKNQIMQILLNLLTNAAEAMTGKGRAITVRLSNPPEADW
                     VAAAIEDEGCGMTPEQKEKIFEPFYTTKAHGTGLGLSIVHRLVTNLHGRLEVESAPGR
                     GTTVTVYFQKYEKSPQEKPEPSDSSLFDTPAEFIPAQLTAPPAKK"
BASE COUNT          746 a         1036 c          873 g          866 t
ORIGIN      
        1 ccacatcatt cagtgacgat gcgcactgga aggcttgtag gcttagccca aactttaaaa
       61 tgtagagact tgcggttgtc actagtatag ggcagatgaa acatatagat aaatcttcct
      121 cgtttaatat ggaaacttgc attcggcatt catccgccac gaggtttgga agggcgtagc
      181 gaagttgaga tagaccatgc gatatggcat ttgggtctac accttgctcg taaagctcaa
      241 ctccgtgagt gcaaaatcgc aattcattgt caatcttata caaaggatgt ttgttctcaa
      301 ggcgttcagt gcataagtct tctaggcaac taatacatcc ggccgttaca tctgactctt
      361 ttggatgctg agagaatatc catcttacgc ccgggtgatt atatttgcac tcaattaaaa
      421 cattaaggtt tccccagtgt cttttcctgc gcttgagaag attaaaagca cgaaggtcaa
      481 ctgagaattc cgtttcaaca tcacgttcgt tcgacctgct gtatgtgtat tcaccggtta
      541 catagagttc gtgtttctcc aacttttccg atactaattg ttgaagaggt aaactacttt
      601 taagcaggtg gtctttccat ttaccagttt gtttcataac catagtcttt ctcattaacc
      661 atttaagttt ctgcttttag ctgtgaacta ctcagcagat gatgtgtcat tttaatcaac
      721 cgtatttcac cagtcaattt ttccttcttt ctccttgccc tatgtaccac tgcaagcggg
      781 ttctccagcc ctgttaattt ggggtcactc tgggggcaca caggtgcccc ttcccgcgct
      841 aatttggggc tgtcataggg gtaaaacgcc ccccgctttt cccgtaggcg aggcttccag
      901 cctcgcggaa ttttggggtc actccggggg taaaacggac aaagtgcgaa atagtaggag
      961 cacttcaggg gccagcggcc agtcggagcg aagcgaagat cccgctaaag gcggggtagc
     1021 caagggccag agttccttgc tcccgaaacg ctttggtgct gtatgtcttt tcgtcttagc
     1081 ctcaactctc agttctcaat cctcgattct gtttttcatc cgctttatct gctgtgaata
     1141 ccgttgtcat tccccaattc ccatctccca aatccgtctt cctctcccac ctcgacattt
     1201 tctattccgc ttcccggaac cggagttatc cacttatccc acaaggagat ccgtcgccaa
     1261 atcgtcggtg atagtcatcc ggagagtccg cctaacttct tgtctcccaa caggagaaaa
     1321 aaattccggg aagcgttttg ttcagcttcc ttgaacagtc gcgaatctga ccggaattcc
     1381 ctgccttcga ttacgaatta cgaggtattg aaaccccgac acctcgacac ttcgacaccc
     1441 cgatacctca atgccgcgac acttcgacac atcgacactt agagtaattc gccgataaac
     1501 aaatagatga ccgcgcccga gcgaaaagcc ctgcctcctg ccggcgggga cgagccgact
     1561 ttagtttttg cccggcacga gcctttgccc gcctggctcc tgcctctacg gcttttgatt
     1621 ttcgttttgc tgtttggaat tgtcttggcc ctcctgcgcc agcccccgca attaataacc
     1681 ccgcttctcg gttacggctt ttgcacgctg gtctttttgg gcatcgtcac acttcaccgg
     1741 cggcgtagca tgccgatggt attgcgtttg gtcttagcgg tgcaaatcgt atgggaagtt
     1801 ttcctggaag ccggtatcgt ccaccagtcg ggcaacctgt ctcaggcgag cccgtacaat
     1861 gcgctctttt tgctcaccat cgtctccgcc agtttggtct accgcctgat cggcacgctc
     1921 gccatcgcca cggcggcgag tcttttctat tccttcgcca tctggggcac caacccggcg
     1981 accctgccct ggccctggga cttttccctg cggcaaatcc tggcggccac cggcgacgac
     2041 tccttcgcgg taatcttcgt gcacatctgc gttttctaca tcaccgcctt catcagcggc
     2101 tacctggccg aaaggctgca gcacaagggc gaagaactgg cggaaacctc cgaacaactc
     2161 cagaaggccc ggctggatac cgacgacatc ctccaccacc tctactccgg acttttgacc
     2221 atcgaccgcg caggtaaggt gatctacttc aaccaggccg cccaggaaat tctggggtac
     2281 cggtcacccg aaattcaggg ccgcgacttc aggcgggtct tcggccaccg gatgcccgat
     2341 ctggtggagc ggctccagca ggtgctggaa tcgggccgga tggaaccccg gttcgaactg
     2401 gttattgtca gccgctccgg ccgccagtgc ccggtgggct tcaccctgtc gcctttgaaa
     2461 gacccgcagg gcaacccgcg cggggtcatc ggtgtttttc aggatttgac cgaaatcaaa
     2521 ctgctggaag accgcatccg cacttccgac cggctggccg cggtgggcga actctccgcc
     2581 ggcatcgctc acgaaatccg gaaccctctg gccgccatcg ccggctcggt ggaggttttg
     2641 cggaaagaac tccaactcac cggcgacgaa aagcggctcc tcgacctggt catccgcgaa
     2701 tcggagcgtt tgaatcagat cctctccgag ttcctccact tcgcccgtat ccgcccgacg
     2761 cttttgacgg ccgttgacct cgcctccgtg acccgcgaaa ccattgaagc tctcaagcgt
     2821 cagccgcttt tttcgggtgg aagcgagttg ggcccccgcc gcaaaattga gatgaaactg
     2881 cagtctgatc cggctctggc cccggtctgg ggcgaaaaaa atcagattat gcagattctc
     2941 ttgaatttgc tcaccaacgc cgcggaagcg atgaccggaa aaggccgggc cattaccgta
     3001 cggctgagca atccgcccga ggcggactgg gtggcggcgg cgatagagga tgaggggtgc
     3061 gggatgaccc ccgagcagaa agaaaaaatc ttcgagccct tctacaccac gaaagcgcac
     3121 ggcaccggcc tggggctctc catcgtccac cggctggtaa ccaacctcca cggccggttg
     3181 gaagtggaga gcgcacccgg ccgcggcacc acggtgactg tctacttcca gaagtatgag
     3241 aaatcgccgc aggaaaagcc cgaaccttcg gactcatctt tgttcgacac gcctgccgaa
     3301 ttcatcccgg cccaactcac cgccccgccc gccaaaaaat agtctgcttt catcctcttc
     3361 ttggccttga ctttttctat tcccgtgtta acttagaggg tgacctttta acccgatccg
     3421 gtgagatcgg gaaaggaaag cctgttgaac ctatgtgtcg tggaaaaatt cgcccctccc
     3481 tctgtcgagc ggagggattt tttatgccgg tgaggtgctc g
//
LOCUS       DBNGZA010000102         6568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_444173, whole genome shotgun sequence.
ACCESSION   DBNGZA010000102 DBNGZA010000000
VERSION     DBNGZA010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_444173"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16FU_02240"
     CDS             <1..410
                     /locus_tag="AC16FU_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HIR2993229.1"
                     /translation="YLAESFMAGDPFQDGGFVIVNGLKYDDDGNLVELDTPYPGGNLF
                     SLASGGAIYIRDPHQKVGEDQLNGGKFTPVTAKDWELIEPYLKENQNLFGITVEQLLT
                     VGGKKMAPSEVYRKIEPVVLRALEPEEMEVARF"
     gene            704..2137
                     /locus_tag="AC16FU_02245"
     CDS             704..2137
                     /locus_tag="AC16FU_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2993230.1"
                     /translation="MIYQKNGADTTAGFGFSIASAGDVDGDGKADFIVGAPQSGTPYT
                     SAGTGSVFVYSGATGILLHQKNGSVLFDNFGHSVAGAGDVNGDGKADFIVGAPFAAPA
                     GVEQAGAVYLYSGGTGSLLFQKNGIDPFDWFGLTVAGVGDVNGDGRADFIVGAPLADP
                     GGVVNAGSIFVYSGATGTLLYRKDGAAFLDNFGRSVAGAGDIDGDGRADFIVGALLAD
                     PGGVEAAGSAFVYSGATGSLLYQKDGADSVDLFGRSVAGAGDMDGDGRADFIVGAPDA
                     NPGGVRDAGTAYVYSGATGDLLFQKNGTDSADLFGFSVAGAGDVDGDGKADFIIGALL
                     ADPGGLTDAGSAYVFSGATGALLFSVNGTAPLDELGHQVASAGDVQGDGKAEVIVGAP
                     ETDPEGIIDAGSAFVYGFCVLRGDVDNSGVADIIDIVGLVRNVVFGDPLPNTVAGDTD
                     CNGIWDISDLVLLVQYVAFGTPTPCCL"
     gene            complement(2165..2238)
                     /locus_tag="AC16FU_02250"
     tRNA            complement(2165..2238)
                     /locus_tag="AC16FU_02250"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:His,seq:gtg)
     gene            complement(2406..2479)
                     /locus_tag="AC16FU_02255"
     tRNA            complement(2406..2479)
                     /locus_tag="AC16FU_02255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2443..2445),aa:Arg,seq:tct)
     gene            complement(2536..2925)
                     /locus_tag="AC16FU_02260"
     CDS             complement(2536..2925)
                     /locus_tag="AC16FU_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2993231.1"
                     /translation="MEKQSKAVVQSYRDLVVWQKAHELTLSVLDLCDSWNTTRAAKII
                     SDQLIRSATSVPANIAEGYGGRKGKEFVSYLYQARRSVTETDYWLLLSRERGLSSAEQ
                     TKLLQSGYAGLIRMINTIIGKLNAKEL"
     gene            complement(2959..3032)
                     /locus_tag="AC16FU_02265"
     tRNA            complement(2959..3032)
                     /locus_tag="AC16FU_02265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2996..2998),aa:Pro,seq:tgg)
     gene            complement(3083..3688)
                     /locus_tag="AC16FU_02270"
     CDS             complement(3083..3688)
                     /locus_tag="AC16FU_02270"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HIR2993232.1"
                     /translation="MRLVLATANADKIQEINSCLKKLPIEILTYRDFPAFPEVAETGK
                     TLEENAKLKARSIHRATGLPALADDTGLEVDALGGAPGVYSSRFAGENATYADNNRKL
                     LSLLASIPLEKRTARFRCVLALALGPDDLRTAEGKIEGVITDQPRGSHGFGYDPIFSI
                     PHLKRTFAELSTAEKNQISHRGLALKKMAALLARLVNAPAS"
     gene            complement(3690..4541)
                     /gene="murI"
                     /locus_tag="AC16FU_02275"
     CDS             complement(3690..4541)
                     /gene="murI"
                     /locus_tag="AC16FU_02275"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR2993233.1"
                     /translation="MPTTLPAPHPDLVGDSRKQPIGIFDSGIGGLTVAAEIFKKLPHE
                     PVIYFGDTGRYPYGMRSAEVIQKFSRQDANFLLTFGVKLIVVACNTASALALEKIKGE
                     YRVPLVGVIEPGARAAVQATRTGRVGVIGTVGTINSDSYARAIRRIDPAVKVFSLPCP
                     LFVPLAEEGFLDKPATRLIAREYLAPLKKKDIDALVLGCTHYPLLKKVISHVMGKQVR
                     LIDSASETAGAVKAELEVMGLSASDGKQSGRQFFVSDTPEKFVQIGRRFLRKHITRAE
                     KIDITQY"
     gene            complement(4510..6054)
                     /locus_tag="AC16FU_02280"
     CDS             complement(4510..6054)
                     /locus_tag="AC16FU_02280"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR2993234.1"
                     /translation="MKRFRTTPFVLVLGLAALSGASADVKVKQGDRSQTFRSTTIEKE
                     EYVTAQDAAAAVDGKVQWDPVQKKVTFFVGEHKLVFSLFSRYVQVDDRVANLHLLPEF
                     QAGTLWLPLRFLAPIFESIRPEEFIWDQNDKTLRVETGKYNVVDLRASEKLNGTLIEI
                     FTTDPLSFETFLSEDVWLSVNIYKGKLNPAELQKKYLGRYVKEIKAYQFDNAAQVSFR
                     LSKPFLQYRESQAADPDRIQIALEDTSLAKSIDTGAFRPRTTRPGPPKPPVVLPQAKP
                     IQVVVVDAGHGGQDTGATGKKKKTKEKDVVLAIAKKVARKLEADGRFRVVLTRTDDTF
                     IPLEERTKIANDRGADLFVSIHANANRNKSLAGFETYFLDQAVTDEARAVAQMENASL
                     RFEQKDTALARLDDLSFILLDMVQNEHQKESEDLAGLIQGELHRHLPIPDRGVNQAAF
                     FVLNRAYMPAVLVETAFISNAKEEQLLRKGEFQDRIAESIYQAVVQFKTKYEKLNAHD
                     SSRASP"
     gene            complement(6051..6521)
                     /gene="smpB"
                     /locus_tag="AC16FU_02285"
     CDS             complement(6051..6521)
                     /gene="smpB"
                     /locus_tag="AC16FU_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR2993235.1"
                     /translation="MSQTDERKIVATNRKARYEYEILETYEAGLVLAGTEVKSLRGGR
                     ANLQEAYAHPLRGELFVYNLHISPYEAGNRENRDPIRPRKLLLHKKEIRRLAGKIAEK
                     GLTLVPLLLYFKEGYAKLELGLGRGKKLYDRRRAIREREMKREIERAHRERFKA"
BASE COUNT         1302 a         1772 c         1931 g         1563 t
ORIGIN      
        1 attatttggc tgagtcgttc atggcgggtg atccgttcca ggatggcggg ttcgtcatcg
       61 tcaacgggct taagtatgat gacgacggca atcttgtcga attagataca ccctatcccg
      121 gcgggaatct cttttcactc gcctcgggcg gggcgattta cattcgcgat ccgcaccaaa
      181 aagtggggga ggatcaactg aacggtggaa agttcacccc ggtcacggcg aaagactggg
      241 agttgattga accgtacctc aaagaaaatc aaaatctctt tggcattacg gttgaacaac
      301 tgctcaccgt tggcggaaag aaaatggcac cctccgaggt ctaccgcaag attgagccgg
      361 tggtgctaag agccctagag ccggaggaga tggaagtggc gaggttttaa agggcaggga
      421 tcagttgaaa gcgcgaaaag ccagcgtagc tctctgaaat ttcctttaca actcaataaa
      481 attcgttgta tatttacttg gcgcgaatgc tgacgggtgg ggcgcctcgc ggaaaaggca
      541 gactgttgtg gacggtcgaa ctacaattca aaagtttttt gccagcgaga actggcggtt
      601 ctgaggagaa taaatgtctg gttttgctcg ttttctttcc tccctctgtt ctggatgcct
      661 cattttgttc tcttcttttt cgtccgctct tagccaggcc agtttgatat accagaaaaa
      721 tggcgccgac actaccgctg ggtttggttt ctccatcgca agtgccgggg acgtggacgg
      781 cgacggcaaa gctgatttta ttgttggtgc tccccaatcc gggacgccct atacctccgc
      841 cggaacagga tcggtttttg tctattccgg cgccaccggc atccttctgc atcaaaagaa
      901 cggctctgtc cttttcgata actttgggca ttcggtggca ggtgccggcg atgtgaatgg
      961 cgatggcaag gccgatttca ttgtcggggc gcctttcgcc gctccggctg gggttgaaca
     1021 ggcgggtgcg gtgtatctct actccggcgg aacgggctcc ctcctcttcc agaaaaatgg
     1081 catcgatcct ttcgattggt tcggccttac tgtggccggc gtcggagatg tcaatgggga
     1141 tgggagggct gatttcattg tcggggcacc gctggctgac ccgggcggag ttgtcaatgc
     1201 cggttcgatt tttgtctatt ccggtgccac cggcacgttg ctttatcgga aagacggcgc
     1261 cgccttcctg gacaactttg ggcgttctgt cgccggggcc ggagacattg acggcgacgg
     1321 gagagccgat tttatcgttg gcgcacttct tgccgacccc ggaggggttg aggcagctgg
     1381 ttcagctttt gtctactccg gtgcgactgg ctctctcctt taccagaaag atggtgccga
     1441 ctccgtcgat ttatttggcc gttccgtagc cggcgccggt gatatggatg gtgatggtag
     1501 ggctgatttt atcgtcggtg ctcccgatgc taatccgggt ggggttaggg acgccgggac
     1561 agcctatgtc tattccggag ccaccggcga cctcctcttc cagaaaaatg gcaccgactc
     1621 cgccgatctg tttggttttt ctgtagccgg cgccggggat gtggatggcg atggcaaagc
     1681 cgattttatc attggcgctc ttcttgcgga cccgggcgga ctcaccgacg ctggctcggc
     1741 ctacgttttt tccggcgcca cgggtgctct ccttttctct gtgaacggca cagcgcccct
     1801 ggacgagttg ggtcaccagg tggcaagtgc cggtgacgtg cagggtgatg gaaaggccga
     1861 ggtgattgtc ggagctcccg aaaccgaccc tgagggaatt atcgatgccg ggtcggcatt
     1921 tgtttacggc ttttgcgtgt tgcgcgggga tgtggataac agtggagtag ccgacatcat
     1981 tgacatagtg ggattggtaa ggaatgtggt tttcggcgat ccgttgccca ataccgttgc
     2041 tggcgatacc gactgcaacg ggatatggga catttcggac ctggtgctcc tggtgcagta
     2101 cgtggcgttc gggacgccca cgccgtgctg cctgtaaaaa gcagggatta ggaatcagaa
     2161 taagggtggg tagagggact tgaaccctca acctcctggg ccacagccag gcgctctaac
     2221 cagttgagct atacccaccg ggaaatacag tgataagtga ttagtgataa aagtcaagag
     2281 caatattcct acctttatct gtctgccggt cgattgtggt ttaagatttt cgcttttcat
     2341 cactcaacac tgtttgactg tggttttcat cactaatcac tccatcaccc atcactgtct
     2401 ttcaataccc ccgggccgat tcgaacggcc ggccagctgc ttagaaggca gctgctctat
     2461 ccgcctgagc tacgggggcg aggtaagaca gtgataaagt gacgagtgat gggtgatgaa
     2521 aatctatctc acagatcaga gctcctttgc attcagtttg ccgataatcg tgttgatcat
     2581 ccggatgagt ccggcataac cactctgcaa cagtttcgtt tgttccgcag atgacaaccc
     2641 gcgctcccgt gatagcagta accagtaatc ggtttccgtc accgaccgcc gggcctggta
     2701 caggtagctg acaaactctt tgcctttccg tcctccatat ccttcggcaa tattggcagg
     2761 gacagaagtg gcggaacgaa tcagttgatc cgagatgatc ttggcagcac gtgtcgtatt
     2821 ccaggagtca cagaggtcga gaacgcttaa ggtgagttcg tgcgccttct gccagacgac
     2881 gaggtcacgg taactctgaa ccaccgcttt agattgtttt tccatcactc gtcacccatc
     2941 actttttcac tgtagtaatc ggggtgagag gatttgaacc tccgacgacc tgctcccaaa
     3001 gcaggtgcgc taccgggctg cgccacaccc cgagaacgtt ccctcaatat agaacggcag
     3061 aaaaggtctt acaaggaaac gttcaggagg ctggagcgtt taccaaccgg gccaggagcg
     3121 ccgccatttt cttgagcgcc agtccgcgat ggctgatttg gtttttttcc gcagtcgaaa
     3181 gctcggcaaa ggttctcttc agatgcggaa tggagaaaat cgggtcgtaa ccgaagccgt
     3241 ggctgccgcg cggctggtcg gttatcactc cctcaatttt accttcagcc gtgcgcagat
     3301 catccggtcc caaagcgagc gccaagacgc agcggaaccg ggcggtgcgt ttttcgagcg
     3361 ggatgctagc aagcaacgag agaagtttgc gattattgtc ggcatacgtg gcgttttccc
     3421 cggcaaagcg ggaggaataa accccgggcg caccgcctaa tgcgtccacc tctaagccgg
     3481 tgtcgtccgc caaagcagga aggccggtgg cccggtgaat cgatctggct ttcagtttag
     3541 cgttctcctc taatgttttt ccggtttcag cgacttcggg gaaagcaggg aaatcccgat
     3601 aagtaagaat ttcaatcgga agttttttga ggcaggaatt gatttcttga attttgtccg
     3661 cattcgccgt ggctaagacc agccgcatat caatactgcg tgatgtcgat tttctcggct
     3721 cgcgtaatgt gcttcctgag gaagcggcgg ccaatctgca cgaatttctc gggcgtatcg
     3781 gaaacgaaga attgacgccc tgactgtttt ccgtcacttg cacttaaccc cattacttca
     3841 agttctgctt taaccgctcc ggcggtctcg gaagccgaat cgatcagccg gacttgcttg
     3901 cccatcacgt gcgagatgac cttcttcaac agcgggtagt gggtacagcc caaaaccagc
     3961 gcatcgatat cctttttctt caagggcgct agatactcgc gggcgatcaa acgcgttgcc
     4021 ggtttgtcca gaaatccttc ctcggctaac gggacgaaaa ggggacaggg gagcgaaaaa
     4081 actttgaccg caggatcaat ccggcgaatc gcccgggcat aagaatcgga gttgatcgta
     4141 cccaccgtgc cgataacgcc gacccgtccc gtacgggtcg cctgcaccgc ggcccgcgcg
     4201 ccgggttcaa tcacccccac caacggcacc cggtactcgc ctttaatttt ttccagcgcc
     4261 agggccgacg cggtgttgca ggcgacgacg atcagtttga cgccgaaagt caaaaggaag
     4321 ttggcgtcct ggcgggaaaa tttctgaatg acctcagccg aacgcatccc gtacgggtag
     4381 cggccggtgt cgccgaagta gatgaccggt tcgtgcggga gttttttgaa aatctccgcc
     4441 gccacggtca ggccgccgat gccggagtcg aaaatgccga tcggttgctt ccggctgtca
     4501 cccaccagat cagggtgagg cgcgggaaga gtcgtgggca ttcaatttct cgtatttcgt
     4561 tttgaactgc actaccgcct ggtagatact ttcggcaata cggtcctgaa actcaccttt
     4621 gcgcaggagt tgttcttcct tggcattgga gatgaaggcg gtttccacca ggacggccgg
     4681 catgtaggcc cggttgagca cgaagaaagc ggcctgattt accccccggt cgggaatcgg
     4741 caggtgccgg tgcagttcgc cctggatcaa tcccgccaaa tcctcggact ccttctgatg
     4801 ctcgttctgc accatatcga gaaggatgaa actcaaatcg tccaggcggg ccagggcggt
     4861 gtctttctgc tcgaaccgga gggaggcgtt ttccatctgg gccacggcgc gcgcctcgtc
     4921 ggtcaccgcc tgatcgagga agtaggtttc aaatccggcc agcgacttat tgcggttcgc
     4981 gttggcgtgg atgctgacga agagatcggc gccgcggtcg ttggcgatct tggtccgctc
     5041 ctccagcgga atgaaggtgt cgtccgtgcg cgtcaaaacc acgcggaagc ggccgtcggc
     5101 ttccagtttc cgcgccactt ttttggcgat ggccagcacc acatccttct ccttggtctt
     5161 tttcttcttg ccggtggcgc cggtgtcctg gccgccgtgg ccggcatcca cgacgacgac
     5221 ttgaatgggc ttggcctggg ggaggaccac cggaggtttg ggcgggcctg gccgggtcgt
     5281 ccgcggccgg aaggcgcccg tgtcaatcga tttggcgagc gaggtatcct ccagagcgat
     5341 ctgaatgcgg tccgggtcgg cggcctgcga ctcgcggtac tgcaggaacg gtttggagag
     5401 gcggaacgac acctgggcgg cgttgtcgaa ctggtaagcc ttgatctcct tcacgtagcg
     5461 gcccaggtat ttcttctgca gttcggccgg gttcagtttc cccttgtaga tattcaccga
     5521 gagccagacg tcttcggaga ggaaagtctc aaaactgagc gggtcggtgg tgaaaatttc
     5581 aattaacgtt ccgttcaatt tctccgaggc gcgcagatcg acgacgttgt atttgcccgt
     5641 ttcgacccgc agggttttgt cgttctggtc ccagatgaac tcctccgggc ggattgattc
     5701 gaatatcggc gccagaaagc ggaggggcag ccagagggtg ccggcctgga attccggcag
     5761 gaggtgcagg ttcgccaccc ggtcgtccac ctgcacgtag cgggagaaaa gtgaaaagac
     5821 gagtttgtgc tcgccgacga agaaggtcac cttcttctgc acggggtccc actggacttt
     5881 accgtccacc gcggcggcgg catcctgggc ggtgacgtac tcctcctttt cgatcgtcgt
     5941 ggaccgaaag gtttgcgagc ggtcgccctg cttgactttg acgtccgctg acgcgccgct
     6001 gagagcggcc aggcctaaaa ccaggacaaa gggggtggtg cggaaacgtt tcacgctttg
     6061 aatcgctccc tgtgggcgcg ttcaatctcc cgtttcattt cccgttcgcg aatggcccgg
     6121 cggcggtcat acaatttctt tccccggccc aggccgagtt ccaatttggc atatccctct
     6181 ttgaaataca aaaggagcgg caccagcgtc aacccttttt cggcaatttt tcccgcgagg
     6241 cggcgaatct cttttttgtg caggagcaac ttccggggcc ggatggggtc acggttctcg
     6301 cggttgcccg cctcataggg agagatgtgc aggttgtaga cgaagagttc accccgcagc
     6361 ggatgcgcgt aggcctcctg cagattcgcc cggccgccgc gcaaagattt cacctcggtg
     6421 ccggctaaca ccaggcccgc ctcgtaggtt tccagaatct cgtactcgta gcgcgccttg
     6481 cggttggtgg cgacaatttt gcgttcatct gtttggctca ttgacttccg ctcatttcca
     6541 atatatcggc taatttccgc cgcgcgca
//
LOCUS       DBNGZA010000103         4481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_656356, whole genome shotgun sequence.
ACCESSION   DBNGZA010000103 DBNGZA010000000
VERSION     DBNGZA010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_656356"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="AC16FU_02290"
     CDS             <1..711
                     /locus_tag="AC16FU_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR2993236.1"
                     /translation="TFQGTEGKMNGGIRPLVAEFVATFAFIFVGAGAICANEFANGDV
                     GLLGIALAHAVALAVMVSATGPISGGHVNPAVTIALWAGGKIGLPRAALYIVAQLAGA
                     AVGALFLRWIFPADVWQAVHLGATEVATNISTGTAIFLEAVLTFFLVFAVYGTAVDER
                     APKSLAGFGIGLVLLFDILVGGPLTGASMNPARTFGPALVGCFWANHVVYWVGPIAGG
                     LLATVVYKFIASRPVSVA"
     gene            925..1530
                     /locus_tag="AC16FU_02295"
     CDS             925..1530
                     /locus_tag="AC16FU_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR2993237.1"
                     /translation="MFRTAVALGVGILLAGCATNKYVNRKFAEVQAANQQTAARVDEM
                     ASRLQAAEANAQQAYALAANAKDEAEKAKILSAAFANYEEIGSKELTFAFDRYVLTEI
                     SHNVLDEIGAMMQQRKDLVLEIAGHTDAIGSDPYNLLLGTNRANAVVRYLNDKFQIPL
                     YRMYTVSYGKSKPKVMAEGRSAQANRRVELRLLGAPGMTNP"
     gene            1947..2708
                     /locus_tag="AC16FU_02300"
     CDS             1947..2708
                     /locus_tag="AC16FU_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR2993238.1"
                     /translation="MEKSFKPAIAEGIASFIFVCCAAGVICANAMTQNAIGWVGIALA
                     NAFAFTALVAFTSHISGGHVNPAVTIGNLFSGRIDFPKATFYIIAQLVGAILAGFVTR
                     TIFPANVWQTVSLGAPILNNPVTTGGAIFIEFLFTCFFVMVAIGCTADDRAPKNLAPL
                     AMGFALGFATLVIGPLTGAGLNPARAFGSAIGSGNWTNQVPYWIGPVLGGICAPILYR
                     ICGGQITKMVSSPWADFESTPTAPEPKVTVKSVNR"
     gene            complement(3068..>4481)
                     /locus_tag="AC16FU_02305"
     CDS             complement(3068..>4481)
                     /locus_tag="AC16FU_02305"
                     /inference="COORDINATES: protein motif:HMM:NF039629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HIR2993239.1"
                     /translation="DYFNAKLIPKVQLQTRFSLTGFQAPTGRSDFVKLYQKLLSARHR
                     NFEVQAGNYFATFGRGMLLRAFQLPGFIYEDRVERIRHEAIRDLFGFRATVTLPLLEA
                     AFVQGKPQDALVTPTGRRTGSLWGGDFRVKPFPRSEALRLGGSFLHLKSQGESEPVAS
                     GVLEANPLQFFGEKVSAKWGADLYVEYALKHPTFQRFFSFGWRDSAYGFYGSMNLSYA
                     RVGLSFEYKDYHNFLLRAGGPVNDPPALIREQSPILLNRETHVLVPEDERGFQIELAA
                     GITTALRVVANLSRAENEIKIGRRKKPVFLERYLEVDYHPLPNWSFRGFADLAQDQFR
                     LQKRRLSGGLAADFDLTKTHSVGFDLQAQKITRDLPFAFIKPDFTNLYLGLKYAWSPL
                     LTVTLSGERSDDPFEDTDTEKGPHDWVNVGLSLRIADSHQIDLFYGRRRGGPACDHGY
                     CISVLPFKGFEMRMTNNLWF"
BASE COUNT          948 a         1216 c         1422 g          895 t
ORIGIN      
        1 acctttcagg gaacggaggg gaagatgaac ggaggaatcc ggccgttagt cgcggagttt
       61 gtcgcgacat tcgcttttat ttttgttggc gcgggagcaa tttgcgccaa tgagtttgcc
      121 aacggcgacg tgggtctcct gggcattgcg ctggcccacg ccgtggcgct ggcggttatg
      181 gtttcggcca ccgggcccat ctccggcggg cacgtcaatc cggcggtgac gatcgcgctc
      241 tgggccggcg ggaaaatcgg tttgccgcgg gcggcgctct acatcgtggc gcagttggcc
      301 ggggcggcgg tgggcgcgct ttttttgcgc tggatttttc cagcggacgt ctggcaggcg
      361 gtgcatctcg gcgcgacgga agtagccacg aatatctcca ccggcaccgc catcttcctc
      421 gaagcagttt tgactttctt tttggtcttt gcggtgtacg ggacggcggt ggacgagcgg
      481 gcgcccaaat cgctggccgg attcggcatc ggcctcgtat tactttttga cattctggtg
      541 ggcgggcctt tgaccggggc ctcgatgaac ccggcccgga ccttcggccc ggctctggtc
      601 ggctgttttt gggccaatca tgtagtatat tgggtgggac ctattgccgg cgggcttttg
      661 gctacggtgg tctacaagtt cattgccagc cgcccggtgt cggtcgcgtg aaagcagtag
      721 ccagtagcca ggtaccagta gccagaagtc aaaggctaag agccaaaaat caagaccagg
      781 aaccagcagc cagggaccag gactaaaacc aggaggaagt gaccagttac cagttaccag
      841 ttaccagtta ccagtagcca ggagccagat tacggaaagt cggaacaagg gatgagcaca
      901 agcaaatcac acaggaggag cagtatgttt cgaacagcgg tggcactcgg ggtggggata
      961 ctactcgccg ggtgcgcaac aaacaagtac gtaaaccgga aatttgcgga ggtgcaggcc
     1021 gcgaaccagc agaccgctgc ccgggtggac gagatggcca gtcgcctcca agcagccgaa
     1081 gccaacgcgc agcaggccta cgctctggcg gccaatgcca aagatgaagc ggagaaggcc
     1141 aaaatcctct cggcggcctt tgccaactac gaggagatcg gctccaagga attgaccttc
     1201 gccttcgacc ggtacgtcct gaccgaaatc tcgcacaacg tcttggatga aatcggcgcg
     1261 atgatgcagc agcggaagga tttggtctta gaaatcgccg ggcacaccga cgccatcggc
     1321 tccgacccgt acaaccttct tttggggacc aaccgggcca acgccgtggt gcgttactta
     1381 aacgacaagt ttcaaatccc cctctaccgg atgtacaccg tcagttacgg caagtcgaag
     1441 ccgaaagtga tggctgaagg ccggagcgcg caggccaacc ggcgggtgga attgcggctt
     1501 ttgggcgcgc cggggatgac aaatccgtaa ccggtaattc gtaactcgta attaaaaccg
     1561 ttgcggcgca ggttggttta tgaaacctgc gccgcttcat ttgggggaaa taaaacgcag
     1621 gtttgtcgct tttttgaaca ggaaggcgac agggcctacg ctacgcggga attgcagcct
     1681 cggttccagg gaacggtcag gctcaaagcc tgacctatgg gataatggta tataatgtaa
     1741 ggaggctcta aacccgcatc tggagcgggg atcgaccctt attaagtttt atggggggtg
     1801 gccgatactt tcactgatcc gactgcgctc accatccgcc tcaggcgggc agttggactt
     1861 gtaaaccggg caaaaagtta ttcccgtccg ccttaggcgg attggtaaca gcgtaaacag
     1921 gagcaacaca caaggaggga gggagtatgg agaaatcgtt caagccggcc atcgcagagg
     1981 ggatagcgag tttcatcttc gtctgctgtg cagccggagt gatctgcgcc aatgcgatga
     2041 cgcagaatgc gatcggctgg gtgggcatcg ccctggccaa cgcctttgcc tttacggctc
     2101 tggtggcgtt cacctcacac atctccggcg ggcacgtcaa cccggcggtg acgatcggca
     2161 acctcttttc ggggcggatt gatttcccga aggcgacctt ttacatcatc gcgcagttag
     2221 tcggggccat cctggccggt ttcgtcaccc ggacgatttt cccggccaac gtctggcaaa
     2281 cggtgagcct gggggcgccg attctgaaca acccggtgac caccggcggg gcgatcttca
     2341 tcgagttcct ctttacctgc ttcttcgtca tggtcgccat cggctgcacg gcggacgacc
     2401 gggcgccgaa gaacctggct ccactggcga tgggattcgc gctgggattt gccaccttag
     2461 tgatcgggcc tctgacgggc gcggggctaa acccggcccg ggcgttcggc tcggccatcg
     2521 gctccggcaa ctggaccaac caggtgccgt actggatcgg gccggtttta ggcggcatct
     2581 gcgcgccgat cctttaccgg atttgcggcg gacagattac caagatggtt tccagcccct
     2641 gggccgattt tgagagcacg cccacggctc cggaaccgaa agtaacggtg aagtcggtga
     2701 accgttagtt cccaacctat cacagggatg tgaaagtgtg gggcacccgg cccccccgga
     2761 tcgatccata cggacgtacg ggagggccgg gttttttatt tcccgggaga gtttcacagc
     2821 agatgaagca gattgagcag atgggaatac taaccggctc agggacgagc tcatacgagg
     2881 cgcaccgacc ggtctaccag cgtagccgag gtttgtcacc tcggcggctc tcctaagctg
     2941 tcatcctacg tcaggccacg ctcgtacgga gcgccgagga tgtaatcctc ggctacgaaa
     3001 cagcggagag acttgctgac ccctcccgtg ccctcctctc ggaggggagg gggcatcaaa
     3061 tgaggaatca aaaccagagg ttgttcgtca tacgcatttc aaagccctta aagggcagga
     3121 cgctaatgca gtaaccgtgg tcgcaggcgg ggccgccgcg gcgacggccg tagaagagat
     3181 caatctggtg gctatcggcg atccgcagac tcaggccgac gttaacccag tcgtgcggcc
     3241 ccttctcggt atcagtgtcc tcaaatggat cgtcgctccg ttcgccggag agggttacgg
     3301 tcaaaagcgg tgaccaggcg tacttcaaac cgaggtagag attggtgaaa tcgggtttga
     3361 tgaaggcgaa gggcaggtcg cgggtaattt tctgcgcctg taggtcaaag ccgaccgagt
     3421 gcgttttggt caaatcaaag tccgccgcca agccgccgct caaacggcgc ttctggaggc
     3481 ggaattggtc ctgcgctaag tcggcgaagc cacggaaaga ccagttgggg agcgggtgat
     3541 agtctacttc caaatagcgc tccaggaaaa ccggcttttt acgacggccg atcttgattt
     3601 cgttttcggc gcgggagagg ttcgccacta cgcgaagcgc ggtggtaata ccagcagcaa
     3661 gttcaatctg aaatcctctt tcgtcttccg ggactaagac gtgggtttca cggttcaaga
     3721 gaatcggcga ctgttcgcgg atcaaagccg gaggatcgtt caccgggccg ccggcgcgca
     3781 aaaggaagtt atggtagtct ttgtattcga aagaaagccc cacccgggcg tacgacaggt
     3841 tcatgctgcc gtagaagccg taggcggagt cgcgccagcc gaaggagaag aaacgctgga
     3901 aggtcggatg ttttaacgca tactcgacat acaggtcggc gccccatttt gcggagactt
     3961 tttccccaaa aaattgcaaa ggattagctt cgaggacgcc ggaggcgacc ggctccgact
     4021 ccccctgact tttcagatgc aggaacgacc cgccgagacg gagcgcttcg ctccggggaa
     4081 agggtttgac ccggaaatcg ccgccccaga gggaaccggt gcggcggccc gtgggcgtga
     4141 ccagggcgtc ctggggtttg ccctggacga acgcggcttc cagcaaaggc agggtgaccg
     4201 tggcccggaa cccgaagagg tcgcggatgg cttcgtggcg gatacgctcg actctgtctt
     4261 cgtaaatgaa gccgggaagt tggaaagcgc gtaacaacat cccccggccg aaggtggcaa
     4321 agtagttacc cgcctgtact tcgaagttac ggtgccgtgc ggatagtagt ttctggtaca
     4381 gtttcacgaa gtcggagcgg ccggtggggg cctggaaacc ggtgagcgag aagcgggttt
     4441 gcagttgaac tttcgggatg agtttcgcgt tgaagtagtc c
//
LOCUS       DBNGZA010000104         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2246925, whole genome shotgun sequence.
ACCESSION   DBNGZA010000104 DBNGZA010000000
VERSION     DBNGZA010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2246925"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1366)
                     /locus_tag="AC16FU_02310"
     CDS             complement(137..1366)
                     /locus_tag="AC16FU_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR2993240.1"
                     /translation="MGYEFFIARRYLRAKRHRHFVSAITLISVLGVVVGTAALIVVLS
                     MMNGFEKEIRTRIVGITGHVTIFDQHNEGVAEYQDLGKRVQALPQVVATAPFVYAKAA
                     IASENENDGILVRGVDAEAEKKVTTIGDEMVAGTFDLAEQPGRLSRIILGRGLAERLG
                     VRTGDTVALFSLRGQSLSAGLTQPKVAKFSVGGLFETGMYEYDASLAYINLTRAQSLF
                     EMDGKVTGLQVKTDDLFGAQKVARALENVLGTAYYAQDWMAMHKNLFSWMALEKYAMF
                     VALSLIIAVAAFNIVSSLIMVVLEKRREIGVLKAMGAKREGIKKIFVLMGLITSSVGL
                     VAGFGLGLLLCWLQQTFKIISLPAEIYFINTLPIDVRPGDFLIVAVAALGISFLATLY
                     PSRRAAALVPAEAIRYQ"
     gene            complement(1367..>2257)
                     /locus_tag="AC16FU_02315"
     CDS             complement(1367..>2257)
                     /locus_tag="AC16FU_02315"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR2993241.1"
                     /translation="TPILQPLYGGALARPFKTHHNTLDLDFYLRIADELYLKRLIIGG
                     FEKVYEFCKDFRNEGMDKFHNPEFTMVELYWAYADYRGILALHQELMLFVARQVFGTT
                     QFAYQGQTIELGGQWEEVPYFDGIKKFTGYDLYQKDEKTVRQAAEKLGIDAAKLTTRG
                     KLLDEIFSAKVEPNLIQPTFVTDFPLEISPLAKKHRDKPELVERYELFIAGLELGNAF
                     SELNDPLDQRRRFEELARLRLLGDEEAAIIDEDFLAALEHGMPPTGGLGFGVDRLVMF
                     FTDQHSIRDVIFFPQMRPEK"
BASE COUNT          484 a          648 c          651 g          474 t
ORIGIN      
        1 aaaagaccgt gcggggtctg gtaactcttt tcgatgcctg aggcggccag caccacacgg
       61 tcgttttcaa cgtatccctc cgcccggctt ctgtcaagag gtatcggcct gttccgggaa
      121 ctcctgactt cggggctcac tggtagcgga tggcttcggc cgggaccagt gccgccgcgc
      181 ggcgggaggg gtagagggtg gcgaggaaac tgatccccag ggcggcaacg gcgacaatta
      241 aaaaatcgcc gggccggacg tcaatcggca gcgtgttgat gaagtaaatt tccgcgggca
      301 aggaaatgat tttgaacgtc tgctgcaacc agcagaggag aagccccagg ccgaacccgg
      361 cgaccaggcc gacgctcgag gtaatcaatc ccatcaagac gaaaatcttt ttgattcctt
      421 cacgtttggc gcccatggct tttaagacgc cgatttcccg gcgcttttcc aggacaacca
      481 tgatcagact cgacacgatg ttgaaggcgg cgacggcgat aatgagactc aaggcgacga
      541 acatggcgta cttctccaag gccatccagg aaaagaggtt cttgtgcatc gccatccagt
      601 cctgggcata gtaagcggtg ccgagcacgt tctcaagcgc gcgggccact ttttgggcgc
      661 cgaagaggtc gtcggttttg acctgcagtc cggtcacctt gccatccatt tcgaagagcg
      721 attgcgcccg ggtcaggttg atgtaggcga gggaggcatc gtactcgtac atcccggttt
      781 caaacaggcc gcccacgctg aacttcgcca ccttcggctg ggtcaaccct gccgaaagac
      841 tctgaccgcg caggctgaaa agtgccaccg tgtcgccggt gcgcaccccc agacgttcgg
      901 ccaggccgcg gcccaagatg atgcgggaga gccggcccgg ttgttcggcc aagtcaaaag
      961 tgccggcgac catctcgtcg ccgatcgtcg tcactttttt ttccgcttcg gcatcaacac
     1021 cgcggaccag aataccgtcg ttctcgtttt ctgacgcgat ggccgctttg gcatagacaa
     1081 agggagccgt agccactacc tgcggcagcg cctggacccg tttccctaaa tcttgatatt
     1141 cggcaacgcc ttcgttgtgc tggtcgaaga tcgtgacgtg gccggtaatg ccgacgatcc
     1201 gggtgcggat ttctttctca aaaccgttca tcatcgagag cacaacgatt aaagccgcgg
     1261 taccgaccac gacacccagg accgaaatca aggtgatggc cgagacgaag tgccggtgtc
     1321 ttttggcccg caggtagcgc cgggcgatga aaaattcgta acccatttac ttttccggcc
     1381 gcatctgggg aaagaaaatc acgtcccgga tcgagtgctg gtcggtgaag aacatcacca
     1441 gccggtcgac gccgaagccc aacccgccgg tggggggcat cccgtgctcc aaggcggcca
     1501 aaaagtcttc gtcgatgatt gcggcctctt cgtcacccaa aaggcgcagc cgggccaatt
     1561 cctcaaaacg ccggcgctgg tcaagcgggt cattcaattc cgaaaaggcg ttccccaatt
     1621 ccagtccggc gatgaaaagt tcgtagcgct ctaccaactc tggtttgtcg cggtgcttct
     1681 tggccaaggg cgagatttcc aggggaaaat cggtgacgaa agtcggctgg atcaaattcg
     1741 gctccacctt cgcggagaaa atttcgtcta agagtttccc gcgggtggtg agtttggccg
     1801 cgtcgatacc cagtttttcc gcggcctggc ggaccgtctt ttcgtctttc tggtacaaat
     1861 cgtatccggt gaattttttg atcccgtcaa aatagggaac ttcctcccac tggccgccca
     1921 gctcaatcgt ctggccctgg taagcaaact gcgtggtgcc gaaaacctgc cgggcgacaa
     1981 agagcatcaa ctcttggtgg agcgccagaa tgccccggta atcggcgtag gcccagtaga
     2041 gttccaccat ggtgaactcc gggttgtgga acttgtccat accctcgtta cggaagtcct
     2101 tgcagaattc ataaactttc tcaaaaccgc cgatgatcaa gcgtttcagg tacaattcgt
     2161 ctgctatccg caggtagaaa tctagatcca gggtgttgtg gtgggttttg aaaggccggg
     2221 ccagcgcccc gccgtacaaa ggctgcagga tcggcgt
//
LOCUS       DBNGZA010000105         1158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1293196, whole genome shotgun sequence.
ACCESSION   DBNGZA010000105 DBNGZA010000000
VERSION     DBNGZA010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1293196"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC16FU_02320"
     CDS             complement(<1..234)
                     /locus_tag="AC16FU_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR2993242.1"
                     /translation="MTNRLRWQFLVGIVISVIFLYLAFRGVKLSELKRALVAVNYWYL
                     LPSIALILASFVVRAYRWGWMVAPIKKAQFGNLF"
     gene            complement(231..>1158)
                     /locus_tag="AC16FU_02325"
     CDS             complement(231..>1158)
                     /locus_tag="AC16FU_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993243.1"
                     /translation="YTFNFSVPGTVRRLLRAEPFDLVVEDINKIPFFSPLFHKRPVLV
                     IVPHLFADSVFREINFILGTYIFLAERPIPAIYRGRRFVVISESTKSDLIRRGIPEDN
                     ITVSFCGIDHTLYSPDKKVAKSGRPTILYLGRLKKYKSVDHLIRALPHVQAKIPDAQV
                     VILGDGDARPRLEKITRVLGLAADVQFLGFVSPAEKVNWLRRAWVAVCPSLKEGWGLT
                     NIEANACGTPAVAADVPGLRDSVNDGTSGLLYRYGDVAQLAERLIRILTDVRLRNNLE
                     RGALAWARKFQWDTAARELAGVIDEVVGRERR"
BASE COUNT          247 a          359 c          332 g          220 t
ORIGIN      
        1 gaaaaggttg ccgaactgtg cctttttgat gggtgcgacc atccaccccc agcggtacgc
       61 ccgtacgaca aagctggcca aaatcagggc aatgctcggc aacagatacc agtagttaac
      121 ggcgaccaac gcccgtttca attccgacag tttcaccccg cggaaggcga ggtagagaaa
      181 tatgacgctg atcacgatgc cgaccagaaa ctgccagcgc agccggttcg tcatcgcctt
      241 tccctcccca ctacttcatc gatcactccg gctaattcac gcgccgcggt gtcccactga
      301 aacttccggg cccaggccag tgcgccccgt tccagattat tccgcaggcg aacgtcggtg
      361 aggatccgga ttaaccgctc cgccagttgc gccacgtcgc cgtagcggta gagcaggccg
      421 ctcgtcccgt cgttgaccga gtcgcgtaaa cccggcacgt cggcggcgac cgccggtgtg
      481 ccgcaggcgt tcgcttcgat attggtgagt ccccagcctt ctttgagcga cgggcagacc
      541 gccacccagg cccgtctgag ccagttgact ttttcggccg gagacacgaa acccaaaaac
      601 tgaacgtccg ccgcgaggcc cagcacacgg gtaatctttt ccaggcgcgg ccgggcatcc
      661 ccgtcgccga ggatgaccac ctgcgcatcg ggaattttcg cctggacgtg cggcagcgct
      721 cggatcagat gatcgacgct tttgtacttc ttcagccgcc ctaaatagag gatcgtcggc
      781 cgcccggact tggcgacctt tttgtccggc gagtaaagcg tgtggtcaat cccgcagaag
      841 ctcaccgtaa tgttgtcctc cggaataccg cgccggatca ggtcgctctt ggtgctttcg
      901 gaaatcacca cgaaccggcg gccccggtag attgcgggta tcggccgctc ggccaaaaaa
      961 atgtaggtcc ccagaatgaa gttgatttcc cggaaaaccg aatcggcaaa gaggtggggc
     1021 acgatgacca gaaccggtct cttgtggaac aacggcgaaa agaaagggat tttgttgatg
     1081 tcctcgacca ccagatcaaa tggttcggcg cgcaggagcc gccgcacggt tcccggcacg
     1141 gagaagttga aagtgtat
//
LOCUS       DBNGZA010000106         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1398989, whole genome shotgun sequence.
ACCESSION   DBNGZA010000106 DBNGZA010000000
VERSION     DBNGZA010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1398989"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC16FU_02330"
     CDS             complement(<1..285)
                     /locus_tag="AC16FU_02330"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR2993244.1"
                     /translation="MEPLDSRTKEYYQASLRPQGEEAVICLDPQGCVTSWSRGAQKLY
                     GFSSEEVLGQSLDILVPEEKRPEMRALLERARSGAEVVRLLVPQKRGEGNS"
     gene            complement(290..>1727)
                     /locus_tag="AC16FU_02335"
     CDS             complement(290..>1727)
                     /locus_tag="AC16FU_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2993245.1"
                     /translation="VAAWLGDSNFWVNHLHPEDRSWALDFCVRATAEKKPHEFEYRMI
                     AADGRTLWVRDLVTVIVENDQPVKLRGVIVDITERKRAEEELNNTLSLLTATLDSTAD
                     GILVVDGDRKIASFNQKFVGMWRIPESVIAARDDHQALAFVLNQLKNPEEFLRKIREL
                     YAQPEGESYDLLEFKDGRIFERYSQPHRIGGKSVGRVWSFRDITELKRQQEELSRTER
                     LRTVGELAASIIHDLKNPMNVILGSAELLNMEGLPAERRGKYCRSIAEQIQRMLILTQ
                     DILDFSRGEMRITPAPVNLLELCEEIADANQAVLARLGIRLTYAGRRESGLSPAFSLD
                     REKFWRVMMNLISNARDALSNGGEIRLRCLIAPAEARLEVEDTGQGVPEEIRDRVFEP
                     FVTYGKTRGTGLGLSIAKKIVEAHGGTITFTTTAGVGATFTASLPRQRVEQVEPGRPA
                     ESPAQGADSTQTIIRERVRAMLKSPEQK"
BASE COUNT          290 a          557 c          514 g          366 t
ORIGIN      
        1 cgagtttccc tctccccgct tttggggaac cagaagccgt accacttcgg caccggagcg
       61 tgcccgctcg agcaaggcgc gcatttccgg ccgcttttct tccggtacca gaatgtccag
      121 actctgcccc agtacctcct cagaggaaaa tccgtagagt ttttgagcac cccggctcca
      181 ggaggtgaca cagccctgcg ggtccaagca aatgaccgcc tcctctccct gcggccggag
      241 gctggcttgg tagtattctt tcgtgcggga gtccagaggt tccatggtct tacttttgtt
      301 ccggtgactt gagcatcgcc cggacgcgct ctcggatgat cgtttgcgtc gagtccgccc
      361 cctgcgccgg gctttcggca gggcggccgg gttcgacctg ttccactctc tgccggggca
      421 gggatgccgt aaaggtcgcc cctactcctg cggtggtcgt aaaggtaatc gtgccgccgt
      481 gcgcctcgac gatttttttg gcgatggaga gccccagacc ggtgccccgg gtcttcccgt
      541 aggtcacaaa gggctcaaag acccggtcgc ggatctcttc cggaaccccc tggccggtgt
      601 cttccacctc caagcgggcc tccgccgggg cgatcagaca gcgcaggcga atctccccgc
      661 cattggagag agcgtcgcgg gcattgctga ttaagttcat cattacccgc cagaatttct
      721 cccggtccag agagaaggcg ggggacaaac cgctctccct gcggccggcg taggtcagcc
      781 gaatccccag acgggccagc accgcctggt tggcatcggc aatttcctcg cagagctcca
      841 acaggttcac cggcgccgga gtaatccgca tttcaccccg cgaaaaatct aagatgtcct
      901 gcgtcagtat cagcatccgc tggatctgct cggcgatgct gcggcagtac ttcccccgcc
      961 gttcggcggg caaaccctcc atattgagca gctccgccga gcccaggatg acgttcatcg
     1021 ggttcttcag gtcgtggatg atggacgcgg ccagctcgcc cacggtgcgg agccgttcgg
     1081 tgcgcgagag ttcctcctgc tggcgtttca gttcggtgat gtcgcgaaag ctccagacgc
     1141 gtcccacgct cttgcctccg atccggtgcg gctgggagta gcgttcgaag atccgtccgt
     1201 ccttgaattc caggaggtcg tagctctcgc cttccggctg ggcgtacagt tcccggattt
     1261 ttcgcaaaaa ctcttccggg tttttcaact ggttcaagac gaacgccagg gcctggtggt
     1321 cgtcccgcgc ggcgatgacc gattcgggga tccgccacat ccccacgaac ttctgattga
     1381 aactggcgat ttttctgtcg ccatccacca cgagaatccc gtcggcggtc gaatccagag
     1441 tggcggtgag gagagacagc gtgttattga gctcctcttc cgcccgcttg cgttcggtaa
     1501 tgtcgactat cacgccgcgg agcttgaccg gctggtcgtt ttcgacaatg acggtgacca
     1561 ggtcgcgcac ccagagcgtg cgcccgtcgg cggcaatcat ccggtattca aactcgtgag
     1621 gtttcttctc cgcggtggcg cgcacgcaga aatccagggc ccagctccgg tcctccgggt
     1681 ggaggtggtt cacccagaag tttgaatcgc cgagccacgc ggcgacc
//
LOCUS       DBNGZA010000107         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1823003, whole genome shotgun sequence.
ACCESSION   DBNGZA010000107 DBNGZA010000000
VERSION     DBNGZA010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1823003"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="AC16FU_02340"
     CDS             complement(<1..259)
                     /locus_tag="AC16FU_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2993246.1"
                     /translation="MSVVAVDLQAFLAEKKKSIDSSLDSFLPKADQEPRRLHQAMRYA
                     VLDGGKRFRPILAIAAFEMAGGRGEVILPAACALELVHTFSL"
     gene            complement(256..519)
                     /gene="xseB"
                     /locus_tag="AC16FU_02345"
     CDS             complement(256..519)
                     /gene="xseB"
                     /locus_tag="AC16FU_02345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR2993247.1"
                     /translation="MPKKQAFEEALKRLEEIVEKLETGDVSLDESLAVFQEGIDLYRF
                     CHKRLTEAEKRVKVLIENVKGEFSLENFEPEGQGEAGADEEKS"
     gene            complement(606..1808)
                     /gene="xseA"
                     /locus_tag="AC16FU_02350"
     CDS             complement(606..1808)
                     /gene="xseA"
                     /locus_tag="AC16FU_02350"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR2993248.1"
                     /translation="MLPKLFTVSELTRRIKENLEAGFPEVWVEGEISNYLLHTSGHRY
                     FSLKDESAQVRCVFWRRQARLLRFEPEDGMKVQIYGNLTVYEKQGQYQLNVLRILPKG
                     RGELELAFQQLKEKLFREGLFDEARKRPLPRFPEAIGIVTSPTGAAIRDLIEILTRRY
                     PPAQIILYPARVQGEDAGEEIEAGIKAFNRHGEADLIIVGRGGGSLEDLWAFNEERVA
                     RAIFHSRIPVVSAVGHEIDYTIADFVADLRAPTPSAAAELVVPEQGQLLAGLRGWLQS
                     LVNELERQVETRRERVVAIGKSYGFRRPLDFIAQRAQEVDEALRVLKFSASHRLDALR
                     GHLALLTGKLDALSPLAVFQRGYAMVLKLPERSLVREARVLQREDQVEVKVFKGRFQA
                     RVEETNVE"
BASE COUNT          414 a          611 c          522 g          428 t
ORIGIN      
        1 ctaaagaaaa ggtgtggacc aattcgagag cgcaggccgc gggcaaaatc acctctcccc
       61 taccacccgc catctcgaaa gcggcgatgg ccagaatcgg ccggaagcgc ttgccgccgt
      121 cgaggacggc gtagcgcatc gcctggtgga gcctcctggg ttcctggtcc gctttcggga
      181 gaaaagaatc cagggaggag tcaatcgact tctttttttc agccaaaaac gcctggagat
      241 caaccgccac cacgctcacg acttttcctc gtcagcccct gcttctccct gtccctcggg
      301 ctcaaagttt tccaagctga attcgccttt cacattttcg attaacactt tgactcgttt
      361 ctcggcttcg gtcaggcgct tgtggcagaa acggtagagg tcgatacctt cctggaaaac
      421 ggccaaggat tcgtctaaag atacgtctcc cgtttccagt ttctcaacga tttcctctaa
      481 tcgtttcagc gcttcttcaa aggcttgctt tttgggcata gtgtccggag tctcttcgga
      541 tgcaggttac ggtgccgggg tagtcgggct cgtctccgag cccgacgggt tagctcaggg
      601 tacactcact cgacatttgt ttcttccacc cgcgcttgaa atcttccttt gaaaaccttg
      661 acctccacct ggtcctcccg ctgcaaaacg cgcgcttctc gaacaagcga ccgctccggc
      721 agtttcagca ccatcgcgta gccgcgctgg aacaccgcca gaggcgagag cgcgtccaat
      781 ttgcctgtca acagcgccag atgaccccgt aaggcatcca accggtggga agcggaaaac
      841 ttgagcaccc gcagcgcttc atccacctcc tgcgcgcgct gggcaatgaa atccagcggg
      901 cgtcggaaac cgtacgactt gccgatggca accactcgtt cccggcgtgt ttccacctga
      961 cgttccaatt cattcaccag actttggagc cacccccgca gtccggccaa aagttgtccc
     1021 tgctccggca ccactaactc cgccgcagcc gagggcgtgg gcgcccgcag atcggccaca
     1081 aaatcggcaa tggtgtagtc gatctcgtgc cccacggcgg agaccaccgg gattctggag
     1141 tgaaaaatgg cccgcgccac tctttcctca ttgaaggccc acaggtcttc gagcgaacct
     1201 ccaccgcgac ctacaatgat gaggtccgcc tctccgtgac gattgaacgc tttgatgccg
     1261 gcttcaattt cttctccggc atcttccccc tgcacccggg ccgggtagag aatgatttgc
     1321 gccggcggat agcgccgcgt aagaatttca atgagatccc gtatggctgc accggtggga
     1381 gaggtcacaa tgccgatcgc ttcgggaaaa cggggcaaag gccgcttgcg ggcttcatca
     1441 aataagccct cgcggaacag tttctccttc aactgctgaa aggccagttc cagttcgccc
     1501 cggcctttgg gcaaaatccg caggacgttt aactggtact ggccctgttt ttcgtagacg
     1561 gtcaaattgc cgtaaatttg cactttcatc ccgtcttccg gttcaaagcg caagagccgc
     1621 gcctgccggc gccagaagac gcatctgacc tgggcgcttt cgtctttgag cgaaaagtag
     1681 cggtgcccgg aggtgtgcag gaggtagttg gaaatttcac cctctaccca aacttcggga
     1741 aagccggctt cgaggttctc tttgatccgg cgcgtcagtt cggagaccgt gaaaagtttg
     1801 gggagcatac aggccccgcc cctggcgggt tcttcataag atacgcttac gtcaccgaca
     1861 ccgtcaaccg gaaaccgtca ggaaaaaatt ggcgaaagcg ctcaaccaaa ggatggagga
     1921 atcttcacca gggtagtcgg gctcgcccca gagcccaacg ttcttttcga ccgct
//
LOCUS       DBNGZA010000108         1468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_550383, whole genome shotgun sequence.
ACCESSION   DBNGZA010000108 DBNGZA010000000
VERSION     DBNGZA010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_550383"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..587
                     /locus_tag="AC16FU_02355"
                     /pseudo
     CDS             51..587
                     /locus_tag="AC16FU_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            632..>1468
                     /locus_tag="AC16FU_02360"
     CDS             632..>1468
                     /locus_tag="AC16FU_02360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2993249.1"
                     /translation="MTPSLTAALDPLTGIFSPIQSDLEAFERLLERSLVSDADLITEI
                     NRHLLARRGKRLRPAIVFLAARLCGDGRGKAVPVFSERAGYAALAIELIHNATLLHDD
                     VVDESPTRRGQATVNAKWKNLIAVLMGDYYFSKAFTLLVKAETEGLMGAVSTATERVS
                     VGQLLEIQESRNFGVTEAAYFRIIQEKTASLFGAAAEAGALVAGAPPAAQLALREYGE
                     RLGNAFQIADDGLDLVGKVEKTGKGLGTDLREGKVTLPLIFALAQAADGNRERIVQLL
                     ENG"
BASE COUNT          279 a          451 c          457 g          281 t
ORIGIN      
        1 gcgcggagat ctacggcgcc gattgttcgt cctacgccca atgccgacgg ctggtcaact
       61 tcacccaaaa acgtttcggc cgcatcgata ttctggtcaa taacgccgcc atctgggagg
      121 cggcgccgat tcaccgcctg acgccgcaac agttggagaa gactctggcg gttaatctct
      181 aaagtatttt ctactgcacg cgcctgacgg ctcccatcat gatgcggcag aagtcgggtt
      241 ccatcgtcaa cctttcctcc accgccgggc agcggggcga agcgttccac tcccactacg
      301 ccgcgacgaa aggggcgatc acgagtttga ccaagtctct ggcagcagaa ctggcgccct
      361 accgcatccg ggtgaacgcg gtggccccgg gctgggtcga taccgacatg agccacggag
      421 cgctcgcagg tcgtgaccgg aaaaaaattc tgggggccat tcccttggga cgggcggccc
      481 atccgcggga gatcgccgcc tgcatcgtat ttgtggcttc ggaggcggcc gcctttgtca
      541 ccggcgagat tttcaacgtc aacggcggcg cggtcttgtg cgggtgaggc tccccttgac
      601 agcccccgca caccgcgtac cttcctccgt catgacgcct tccctcaccg ccgcgctcga
      661 tcccctgacc gggatttttt ctccgattca gtctgatctg gaagcgtttg aacgcctcct
      721 ggagcggtcg ctcgtaagtg acgccgacct gatcaccgaa atcaaccggc accttctggc
      781 gcgccgggga aaaaggctgc ggccggcgat agtttttctg gcggcgcggc tgtgcggcga
      841 tggacgcgga aaagcggtac cggtattttc cgaacgtgcg gggtatgcgg ctttagccat
      901 tgagttgatc cacaacgcca ctctcttgca cgatgacgtg gtggacgagt ctccgacccg
      961 acggggccag gccacggtga acgccaagtg gaaaaatctc atcgcggtgc tcatgggcga
     1021 ctactatttc tccaaggcct tcaccctttt agtgaaagcc gaaaccgaag gtctcatggg
     1081 cgcggtctcc accgccaccg agcgggtgtc ggtggggcag cttttggaaa ttcaggagag
     1141 ccggaacttc ggcgtgaccg aagccgccta ctttcggatc atccaggaga agaccgcctc
     1201 gctcttcggc gccgccgccg aagcgggggc tctcgtggcc ggggcaccgc cggcagcgca
     1261 actggcgctc cgggagtatg gagagagatt gggaaacgct tttcagattg ccgacgacgg
     1321 cctggatctg gtcggcaagg tggagaaaac cggcaagggt ctgggaaccg acctgcggga
     1381 aggaaaagtg acccttccgc tcattttcgc tctggctcag gccgcggacg gcaaccggga
     1441 gcgcattgtg cagctcttag aaaacgga
//
LOCUS       DBNGZA010000109         9368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1931529, whole genome shotgun sequence.
ACCESSION   DBNGZA010000109 DBNGZA010000000
VERSION     DBNGZA010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1931529"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC16FU_02365"
     CDS             complement(<1..729)
                     /locus_tag="AC16FU_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR2993250.1"
                     /translation="MSAELGSMLVKAGKITEAQLTRALEIQKNSKEKLATILVKIGAL
                     ADEDDVSEFIGRQLNMGTLRLADIELNPDVVKLIPLDIARKFNVIAISKLGKTLVVAI
                     SDPNNIYVLDAIKFITGCAIQPVISPEKAIQKAIEHYYQDTGNLKEILGQIEGEGLEI
                     VEAGEGEAPSEQDLQSAVQDKPLVKLVDHLIADAIRKGASDIHLESYEKRIRVRYRID
                     GTLREMAPLPFKYRAAIVSRVKIMA"
     gene            complement(808..1290)
                     /locus_tag="AC16FU_02370"
     CDS             complement(808..1290)
                     /locus_tag="AC16FU_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR2993251.1"
                     /translation="MANEALVLYEEQMALIDRTLAKLLKAAEAKCALLVDKDGHLVTR
                     QGFTHSLDTTALAALLAGSFASTREIARLVGEPEFSVLFHQGKRDHIHINLVGERSIL
                     VVIFDDRTTIGMVRLYAKEISDELVKILSAKKKGHKGPAITADFAASAESRLDDVFQD
                     "
     gene            complement(1772..2350)
                     /locus_tag="AC16FU_02375"
                     /pseudo
     CDS             complement(1772..2350)
                     /locus_tag="AC16FU_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(2358..2696)
                     /locus_tag="AC16FU_02380"
     CDS             complement(2358..2696)
                     /locus_tag="AC16FU_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR2993252.1"
                     /translation="MYHVLESLNKTTGITGSMLVGNDGIVIAADLTEQIDSETIGALA
                     ASISANVQKSLDRLQQTPLRQITIEAQKGKLFLTDVGRGVLVVTTEPQVNIGLVRLEI
                     KEAAQKILRA"
     gene            complement(2671..3654)
                     /locus_tag="AC16FU_02385"
     CDS             complement(2671..3654)
                     /locus_tag="AC16FU_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993253.1"
                     /translation="MARNRREGRLVWVWSVVLALVPLVLYPNSLGTAGWLLGPQRLLF
                     EAVFYLVAFSFLFAEAGLQRLLRPLFVSWIFRGAIGLLFGLAAAVVHGTAVIEALLDG
                     WYRYLPALFLQALATPFVLRGWVGRWLVEARAAARLATFEPAPGPLPVPATGVPHHRT
                     PRPQPYLAASVEPVAASVQKVGREVTWEDAVRFVGEHAGVIKCFLVDEEGLTIASWFR
                     DGAVEDDLWAGLSLRFWEANAGAIARAGARQVTKLEVHTEQEKVFLNRIDLFTLVAVA
                     EPDADELVNVRLARATEMIAAYWHDRAEVLKSHLKTTGQTEEHHVSRVGVT"
     gene            complement(3657..4520)
                     /locus_tag="AC16FU_02390"
     CDS             complement(3657..4520)
                     /locus_tag="AC16FU_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993254.1"
                     /translation="MLNLAEIDERIQKCEKILSENPGSQIFAALADSYRRRGLLDRAF
                     AICREGLRRHPNYGPGHLVMAKLHLERRMFELAEREARLAMELEGRTRVTEILLAEIY
                     LGHGRFADALALLEPLAATGPRDPQIKTLITKAKIGAQKSRTVPVRTKAPAAEVEPEP
                     RPVPLDPAGALQLLRDFAGVAGAAIVSREGLVLEQVGLPKNLAEAVGAVASAIFGSTA
                     ASLPEPVFGSCEQLWIETDAARYWIAAVVEKIWVVVCRPDINFGSFKLHAEEIKRRVR
                     DDAPQHTPLEV"
     gene            complement(4535..4912)
                     /locus_tag="AC16FU_02395"
     CDS             complement(4535..4912)
                     /locus_tag="AC16FU_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993255.1"
                     /translation="MVRGLASFIAICVILVAAVPVAAEVTAAPSRSFLGAIFELVSAG
                     VAALCLLACFKLSTSTKGGEVASSWQVAAGSFLLFTLGELVAFLSQIGVLPPVESWVA
                     LFRVAAIGAFFWALTRMRKVFST"
     gene            complement(4923..6113)
                     /locus_tag="AC16FU_02400"
     CDS             complement(4923..6113)
                     /locus_tag="AC16FU_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR2993256.1"
                     /translation="MSFAGNLATVTFADILQLLSTGKKSGALVITRGSQSKEIFFRNG
                     HVIYATSTNADEDLLGQLLVKRGTIKQADLDRALALQKTSGKKLGATLIELQLISQQE
                     MAACLKIQIEEIVYSLFGWNQGEFNFHEGRTPSANTLVTELNTMNIVMEGMRRIDEWG
                     QIQKNLPPDNIPLKVTMPPPVKGEVPLSADEISLLTLVNGQRTLSDLIRESPVGEFAT
                     TKAIHRLVSAGLLEKSNAPRLLVSEEKNEEHLWTLMLKVYASAFAVVARTLQQKLGEA
                     GQHLYRNVPEKTARGMDAQHVQFVFGEGFDHKALFAALAGVPREIRLFSLCNRFNRLL
                     AEALTVVQCALGGAVKKQVVAEIKREIGLVLGESRSLATAFQLEGELTSTLRQLAGRD
                     GYRG"
     gene            complement(6223..8550)
                     /locus_tag="AC16FU_02405"
     CDS             complement(6223..8550)
                     /locus_tag="AC16FU_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR2993257.1"
                     /translation="MNQPALDSAAALAGGILLGSAVNLSGVLLFLVTVGLVGLGFFAH
                     WHQRPRLATVTLLGVLVAAGMMRYGQVTRDFPLNHISHFVHLGKSLTVTARITDLPDV
                     FNDRTYLTVVVDSLSDGRKRVAASGKISVRLREPTSAFAYGDRIRFSGTIWPPPEAKN
                     PGAFDYQRYLQAQEIFGLVYLSSVNLVAVQRGSELRGLKSFLEKSRAGIIRQFQTNFS
                     HEESALLAGFLLGYTRDIPSEIYRLFRDTGTLHLLAVSGANVGLVVGLVWFLARLFLF
                     PRRLTVVLVLAAIWFYAGLALFQPSVIRAAIMWSLGALAFWFYRTPKAVNIIGVAALG
                     MLGFKPLWLFDVGFQLSFAATLGIVVFVPQVAEWVNRLPGYIRLPARFSLLPLAVSLI
                     AQLATWPFIVYYFHRVSIVSPLANLCIVPLASLAMYSTIPFLAAAKIFPPLGHLVAGA
                     AAKILGLIFFFEERFGADRAAAHVLGGCDLLCFAALGVLAGSLLALTFFPHRRWLSAA
                     LVLGMLNFLAWRSVLHESPSVRATFLEASPGRWLVVQAGSTAALFGSGGRSQIRYVVV
                     PFLAQAGIQRVTACFPFDGGDSTRRALEELCREVTVNRFVWPQAAGERRADFPERAVA
                     GRSGDSLAIVGIVAQPAPMLSGYLCRTRYFSILSVVRADWVTQTDFWNRLAVFAPTVV
                     SLPIQAASPEVLDALARQAPATVVLVEGRQRFGWEREQMWRGATGSQGRLPQHLMILS
                     SCGAVTLAATEKRLRAESYLGGRFLSRTVPAVHNR"
     gene            complement(8765..9355)
                     /locus_tag="AC16FU_02410"
                     /pseudo
     CDS             complement(8765..9355)
                     /locus_tag="AC16FU_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
BASE COUNT         2049 a         2886 c         2710 g         1723 t
ORIGIN      
        1 ggccatgatc ttgacgcgcg agacaatggc cgcccggtac ttgaagggca gaggtgccat
       61 ctcgcggagg gtgccgtcga ttcggtagcg gacccggatc cgtttttcgt aactctcgag
      121 gtgaatatcg gaggcgcctt tacggatggc gtcggcgatc aggtggtcga ctaacttgac
      181 taacggtttg tcctgcaccg ccgattggag gtcttgttcg gacggtgctt ccccttcccc
      241 ggcctcgacg atctctaaac cttctccttc gatctgaccc agaatttctt tgaggttgcc
      301 ggtatcctgg tagtagtgct caatcgcttt ctgaattgct ttttccggac tgatcaccgg
      361 ctggatggca cagccggtga tgaacttgat ggcgtctaaa acgtagatgt tgttggggtc
      421 agagatggcc acgaccaagg tcttgccgag cttggaaatg gcgatgacgt tgaacttgcg
      481 ggcaatgtcc aagggaatga gcttcaccac gtccggattg agctcgatgt cggccagccg
      541 cagcgtgccc atattcaact ggcggccgat gaactccgaa acgtcgtctt catcggctaa
      601 cgcaccaatc ttgaccagaa tggtggccag cttctctttg ctgttcttct gaatttcgag
      661 cgctctggtc agctgggctt cggtaatctt ccccgctttg accagcatgc tccccagttc
      721 agccgacatc cgacactcgt ttcatccgaa gtgaaatgct tgccgcgaag ggcagtggct
      781 accccgcctt ggacggggtc aaacggttca gtcctggaac acgtcgtcca gccggctttc
      841 ggctgaagcg gcaaaatcgg cggtgatggc cggccctttg tgtccttttt tcttggccga
      901 aagaatcttc accagttcgt cggaaatttc cttggcgtac agacgcacca tgccgatggt
      961 ggtccggtcg tcaaagatca cgaccaggat gctccgctcg cccaccaggt tgatgtggat
     1021 gtgatcgcgt ttgccttggt ggaacaagac cgagaactcc ggctccccca ccagtcgggc
     1081 gatttcgcgg gtggaggcga aactgccggc caagagcgcc gccagggccg tggtatccag
     1141 cgaatgggta aaaccctgcc gggtgaccag gtgcccgtcc ttatcgacca ggagcgcgca
     1201 tttcgcctcg gccgccttga gcagcttggc cagggtacgg tcaatcaagg ccatctgctc
     1261 ctcgtagagt actaacgctt cgtttgccat cgtgacctcc ttacaggtat cgcaacgacc
     1321 ccgggaacta cctccccaac gaaaacaatc gtgaccagaa cgactttttc ttcttcttcg
     1381 gtggcagtgg ccgcaaccgc ggccgggagg ccaccaattg cgccggcgtc aaaaccggtt
     1441 ttcccaccgg atctgccgag gaatctggcc gccgcacagg cgcaatcggt ccggaactat
     1501 ttcccgaaaa ccgttccgga tagagccgtc caatcaccgg ttccggagcg ccgcccggac
     1561 gtgggcggtg cggaagcact ggcgctcggg ccgccacaga ctccgctttg aacatggttg
     1621 cggcgacagg tgcgcccacc cccaccggct cttccagtgt gcgtggctgg accgaactcc
     1681 cgggcccagc cgccgggacc gccggtgtca cctgcttctc aaccgccgcc ggcatgtcgg
     1741 tctgggtcac ctcggaattt ttctccgtct ggcggcgcgc tttatcgagc accaacttgg
     1801 tgacgcattt gagtgtatca aacacaccaa tccctttggt ggcgacggct tcaaacgagg
     1861 ggagtctttt agggttgagt gcctgctcca gttgctccaa agggacagcc ccggggaggt
     1921 cgcgcttgtt atactgaaaa accatgggaa tttcttcagg ttcgtaccca tattcgcata
     1981 aattctcata caaattccgc aggctttcga tattctcatc ggccttctcg gcctgcgaat
     2041 cggccacgaa gacgagcccg tccgcgcccc gcagcaccag cttgcgcgtg gcattgtagt
     2101 agacctgccc gggcacggtg tagagctgga atttggtggt gaacccggcc accgacccga
     2161 tgttgatcgg caggaaatcg aagtagaggg ttcggtccgc atcggtggcc aaagaaatca
     2221 actccccttt cgtattcggc ggcaccttct tatggatgta ctgcaagttc gtggtcttgc
     2281 ccgacaaccc cggaccgtag aagacgatct tgcagtgaat ctcccggaag gcgtaattga
     2341 tcgagaccat gggaaggtta cgcccggagg attttctgcg ccgcttcctt aatctccagc
     2401 cgtaccagac cgatgttcac ctgcggttcg gtcgttacca ccaagacgcc ccgacccaca
     2461 tcggtcaaaa agagttttcc cttctgcgct tcgatggtaa tctgccgcag gggggtctgc
     2521 tgcaagcggt ccaaggactt ctgcacgttg gccgaaatcg aggcagccaa ggcgccgatg
     2581 gtttcgctgt caatttgctc ggtgagatcg gcggcgatga cgatgccgtc gttgcccacc
     2641 agcatactgc cggtgatccc ggtggttttg ttaagtgact ccaacacgtg atacatggtg
     2701 ttcctccgtc tgtcccgtcg ttttgaggtg ggatttgagc acttctgcgc ggtcgtgcca
     2761 gtaggcggcg atcatctcgg tggcccgggc cagccgcacg ttgaccagct cgtcggcgtc
     2821 cggttccgcc accgctacca gggtgaagag atcaatacga ttcaagaaaa ccttttcttg
     2881 ttcggtgtgg acttcgagct tggtgacctg ccgtgctccg gcacgggcaa tcgccccggc
     2941 attcgcttcc cagaaccgga gggacaaccc ggcccacagg tcgtcttcga ccgcgccatc
     3001 ccggaaccaa ctggcgatgg tcagtccttc ctcgtcaacc agaaaacact tgatgacccc
     3061 ggcatgttcg ccgacgaacc gcaccgcatc ttcccaggtg acttcccggc ccaccttctg
     3121 cacgctggcc gccaccggtt cgaccgaagc agcgaggtac ggttgcgggc ggggtgtccg
     3181 gtgatggggt acgcctgttg ccggtaccgg cagaggcccc ggagccggtt caaacgttgc
     3241 caggcgggca gccgcgcgcg cttcgactaa ccaacgcccc acccaccctc tgaggacaaa
     3301 gggagtagcc agcgcctgga gaaacagcgc tggcaggtag cgataccaac cgtctaagag
     3361 cgcttcaatc accgcggtgc cgtggaccac ggcggcggcc aaaccgaaca aaagaccgat
     3421 agcgccgcgg aaaatccagc tgacgaagag cggacggagg agccgttgca acccagcctc
     3481 ggcaaacaga aacgagaaag cgaccaggta gaacaccgct tcgaagagca gccgctgcgg
     3541 tcccaaaagc caccccgccg tccccaggga attcgggtac agcacgagcg gcaccagtgc
     3601 caacaccaca ctccagaccc aaaccagacg accttctctg cggttacgtg ccatcgttac
     3661 acctcgagcg gcgtgtgttg gggtgcgtcg tccctcaccc gtcgtttgat ttcttcggcg
     3721 tgcagtttga acgatccgaa gttaatgtcg ggccggcaga cgacgaccca aattttttcg
     3781 accaccgcag caatccagta gcgcgccgcg tcggtttcaa tccacagctg ctcacagctc
     3841 ccgaagaccg gctccggaag gctggccgcc gtggaaccga agatggcgct ggcgaccgcc
     3901 cccacggcct cggctaagtt tttcggcaaa ccgacctgtt cgagcaccag gccttcccgc
     3961 gaaacgatcg ccgccccggc cacgcccgca aagtcgcgaa ggagctgcaa ggctcccgca
     4021 gggtcgagcg gtaccggacg aggctccggc tcgacttccg cggccggcgc cttcgtcctg
     4081 acgggcaccg tgcgggattt ctgagcgcct attttcgctt tcgtgatgag cgttttgatc
     4141 tgaggatcgc gcgggccggt cgccgccaaa ggctccagga gcgccagcgc gtcggcaaag
     4201 cggccgtgcc ccaggtaaat ttccgccaaa aggatttcag tcacccgggt ccgcccttcc
     4261 agttccatgg ccagccgggc ctcgcgttcg gccaattcga acatgcgccg ctccagatgc
     4321 aacttggcca tcaccaggtg tccgggacca tagttcggat gccggcgcaa gccctcccgg
     4381 cagatggcaa aggcccggtc taagagcccg cgccgacggt aagaatcagc cagcgcggcg
     4441 aagatctgcg agccgggatt ctcgcttaag attttctcgc acttctgaat gcgttcgtcg
     4501 atttcggcta aattcagcat ccggcactca cgcttcaggt actgaaaacc ttccgcatcc
     4561 gggtcagggc ccagaagaac gctccgattg cggccacccg gaacagggcg acccagctct
     4621 ccaccggcgg gagcacacca atctggctta agaaagccac gagttcgcca agagtgaaaa
     4681 ggaggaatga acctgcggca acttgccagc tggaggccac ctcaccccct ttggtggagg
     4741 tggacagttt aaaacaggcc aaaaggcaca gcgccgcgac gccggcggaa accaactcga
     4801 aaatcgcacc gaggaacgac cgggacggag ccgcggtaac ctcggccgcg accggcactg
     4861 cggccaccag tatgacgcat atcgctatga aactcgccag gcctcgtacc atatgttcct
     4921 cctcatcccc gatacccgtc gcgaccggcc agttggcgca gcgtgctggt cagttctcct
     4981 tccagctgga aggcggttgc cagacttcgc gactcgccca gaaccaaccc gatctcccgt
     5041 ttgatttctg ccaccacctg ttttttcacg gcgcccccca gcgcacactg taccaccgtc
     5101 agggcctcgg ccaaaagccg gttgaaacgg ttgcagagac tgaagagccg aatttcccgg
     5161 ggcacgccag ccagggccgc gaacagggcc ttgtggtcaa aaccttcacc aaagacgaac
     5221 tgcacgtgtt gcgcatccat gccgcgcgcg gtcttttccg gaacattccg gtacaggtgc
     5281 tgacccgctt cccccaactt ttgctgcagg gtgcgggcca cgaccgcaaa cgcgctggcg
     5341 tagactttga gcatgagcgt ccagaggtgc tcctcgttct tttcctcgga aaccaaaagc
     5401 cgcggcgcgt tgctcttttc cagaaggccg gccgacacca gccggtggat cgctttggtg
     5461 gtggcgaatt ctcccaccgg actttcgcgg attaagtccg acaacgtccg ctgtccgttg
     5521 accagagtta acagggaaat ttcgtcagcc gacaagggca cttcgccttt caccgggggc
     5581 ggcatggtca ccttcagcgg aatgttatcc ggtggcaggt tcttctggat ctgcccccac
     5641 tcgtcgatcc ggcgcatccc ctccatcacg atgttcatgg tgttcagctc ggtgaccagc
     5701 gtattggcgg agggggtccg gccttcgtgg aaattgaact ccccctggtt ccagccgaac
     5761 agactgtaca cgatttcttc aatctgaatt ttcaggcagg cggccatctc ctgctgactg
     5821 atcagttgca gctcgatcag ggtggcgccc agtttcttcc cggaagtttt ctgcaacgcc
     5881 agggcccggt ccagatcggc ctgcttgatg gtgccccgct tgactaacaa ctgacccaaa
     5941 agatcttcat cagcgttggt cgatgtagcg tagataacgt gcccgttgcg aaaaaaaatt
     6001 tctttgctct ggctccccct ggtgatgacc aaggcaccgg acttcttgcc ggtggagagc
     6061 agctgcaaaa tatcggcgaa cgttaccgtt gcgagattgc ccgcaaaact catcgcacca
     6121 tcctcgtacg ccccgcgcgg ggaacggcgg cacaaacgcc gacacactcc gcccgtagca
     6181 gagccatctt ctgtttatgt atcgggatag agaaaggttt tcttaacggt tgtgcacggc
     6241 gggcacggtt ctgctcaaga atcgaccacc gaggtaactt tcggcccgga ggcgcttttc
     6301 ggttgccgcc aaagtcaccg cgccgcagga agataaaatc ataaggtgct ggggtaggcg
     6361 cccctgactg ccggtcgccc cccgccacat ctgctcacgc tcccaaccga accgctgtcg
     6421 gccttccacg agcacgaccg ttgccggcgc ctgccgggcc agtgcgtcca gcacctccgg
     6481 ggaagcggcc tgaatcggca gggacaccac tgtgggcgca aaaacagcca gacgattcca
     6541 gaaatctgtc tgggtgaccc agtccgcgcg cacaacactc agaatggaaa agtatctggt
     6601 ccgacaaagg taaccggaga gcattggcgc cggctgagcg acaatcccca cgatggccag
     6661 agaatctccg gaacggccgg cgacggccct ctccggaaaa tcggctcgtc tctctcccgc
     6721 tgcctgcggc cagacaaaac ggttaaccgt tacttcccga cacagttcct ccagcgcccg
     6781 tcgggtggaa tcgcccccat caaaagggaa gcaggcggta acccgctgga tacccgcctg
     6841 tgccagaaag ggaacgacga cgtagcggat ttggctcctg ccgccgctgc cgaagagcgc
     6901 cgcggtggag ccggcctgaa cgaccagcca gcgtcccggg ctggcctcca gaaaggttgc
     6961 tcgaaccgac ggcgattcat gaagcaccga acgccaggcc aaaaaattga gcatccccag
     7021 caccagcgcc gcggacagcc agcgccggtg cgggaagaag gtcagagcca atagcgatcc
     7081 cgcaagcacc ccgagcgctg cgaaacacag gaggtcacac ccacccaaaa cgtgcgccgc
     7141 agcccggtcg gcaccgaatc gttcttcaaa gaaaaaaatc aaaccgagaa ttttggccgc
     7201 cgctcccgcc accaggtgac ccagcggcgg gaaaatcttc gcggctgcta gaaaagggat
     7261 agtcgagtac atcgccagac ttgccagggg cacaatgcag agattcgcca aaggcgagac
     7321 gattgatacg cggtggaaat agtagacgat gaagggccag gtcgccagtt gcgcgatgag
     7381 cgagaccgcg agagggagaa gcgaaaatcg cgcgggcagg cgaatatatc cgggcaagcg
     7441 attcacccat tcggcaacct gcggaacgaa taccacgatt cccaaagtag ccgcaaaaga
     7501 aagttgaaat cccacgtcaa acagccagag gggcttaaaa ccgagcatcc ctaaggcggc
     7561 gaccccgatg atattgactg ccttgggtgt tcggtaaaac cagaaggcca gcgcccccag
     7621 actccacatg atcgccgcgc gaatcaccga aggttggaag agcgccagcc cggcataaaa
     7681 ccagattgcc gcgagcacca ggaccacggt caagcggcgg gggaaaagaa agagacgcgc
     7741 caaaaaccag accagcccca cgaccaagcc gacattggcc ccgctgactg ccagcagatg
     7801 cagcgtaccg gtatcgcgga agagacggta gatttccgac ggtatgtctc gggtgtagcc
     7861 caagagaaac ccggccagaa gcgcgctttc ctcatgagaa aaattggttt ggaactgccg
     7921 gatgatcccg gcccggcttt tttccaaaaa tgatttcagt ccacgcagct cagagcctcg
     7981 ctgtaccgca accagattca ccgaggaaag gtacaccagc ccgaaaattt cttgggcctg
     8041 gagatacctt tgatagtcga acgcacccgg gttctttgcc tcaggcggcg gccagatggt
     8101 gccgctaaac cgtatccggt cgccgtaagc gaaagcagaa gtcggttccc tgaggcgaac
     8161 agatattttg ccgctggcag caacccgttt tctgccgtcg gaaagggaat cgactacgac
     8221 cgtcaggtag gtgcgatcgt tgaagacgtc gggaagatcg gtgatgcgtg ccgtgaccgt
     8281 cagagatttt ccaagatgaa cgaagtggct gatgtggttc agcggaaagt cgcgggttac
     8341 ttgcccgtag cgcatcatcc ccgccgcaac cagaacaccc aagagggtga ccgttgccag
     8401 ccgtgggcgc tggtgccagt gggcaaaaaa accgaggccg accagtccga cggtgaccaa
     8461 aaagaggagc actccggaga ggttcaccgc ggaacccaaa agaatccctc cggccagggc
     8521 cgctgcgctg tccaaagccg gctgattcat gcttctgctt tctgatcaaa cgcaggaaaa
     8581 gaccggaaac ctgctgccat tcaggaatcc gcaggagttt gccggccagg ccgtaggtca
     8641 gcgagaagag actgagtaag atcgccacgg tgatgaaacg accaatgacg gtgtgggcag
     8701 ccgaaaaata cggaaaaagt cggggtgtgc cccagccaat caagccggcc aatagcgcag
     8761 cggccaaaac ccggctggca cccgctccca ttgtccccca gggaagtgcg cccagccgaa
     8821 agtgggcaaa ataaaacaaa ccgcagacat tcagagcagc ggaggctgaa gttgccagcg
     8881 ccagcccgcg aaaccccaga ggttgcatga gcactaaatt caaaccaata ttgaaggcca
     8941 cggccagggc tccaatcgct accgggattt tgggcaacct ggcggcgtag aacgtttgcg
     9001 ccagaacccg caccgacgca tagaagacta aaccgagcgc atagaaccag agggcgctgg
     9061 cggtggcgag agtatcggcc cgggtgaagc ggccgtactg gtacaagagc gccaccagag
     9121 gtttgccggc caccaaaagg aaaaccgtgg ccggaatggt gaggaaaaaa accaaccgga
     9181 gggaactagt gacggcggcg agcatcccgg tccggtcacc ctgcgctgct aaacgcgaga
     9241 ctaaaggaag cgtcaccgtg gctacggcaa tgccgaaggc acccagagga aaatgcatca
     9301 gccgaaaact gtagttgaga taggaaacgc tcccctgggg taaaagcgag gccacaagcg
     9361 tattgacg
//
LOCUS       DBNGZA010000110         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2564863, whole genome shotgun sequence.
ACCESSION   DBNGZA010000110 DBNGZA010000000
VERSION     DBNGZA010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2564863"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="AC16FU_02415"
     CDS             complement(<1..281)
                     /locus_tag="AC16FU_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993258.1"
                     /translation="MAPVGKTFFLIVVTFLAGRNVSFAYPKIYGNYQFNFQRQLLPLG
                     SNNLYNQILYFNLQDYLMVKNRFLFTAYVVRNEYVSNSKRVDFRPRLDF"
     gene            complement(281..2422)
                     /locus_tag="AC16FU_02420"
     CDS             complement(281..2422)
                     /locus_tag="AC16FU_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2993259.1"
                     /translation="MKRSAWAIGSLLGFVAALLVRAQTGNYDTSPHGDPNTGVLRDNR
                     FARGECSQCHLAHKDITPEALLLFTENNNNLCYTCHSQKPIGYPAQESDRLPIASTYP
                     GYFEYNSNGVKITGVENRKRWPGQLVYENSGSSASGKYFSPHRSDLDMPRKDNNGSGM
                     CLNCHNPHGTRNPFDMLDTTYLNIQGAFVSGRPGNYALCFKCHSTDGPAGMNPENKRI
                     ADYYDKSVNNDGESGHQFKTTFGYVRAGDKLPCYDCHNPHGSQGNDGLHPNTFLLSDQ
                     RAGWSGLDSLQTSSTQVRRFCFGCHKSSDGQGGGTVEGVTLAALPSSVAEHAFNDQTH
                     CYECHGRDYSSPTTRNVHHPLPGGDCLSCHSMAQGDPASPTRRRAITGQAGDFVKVAH
                     HVTNGTGIEVVTKFDCGVCHMEGDPLTGRTNSTYHKNGVLDLRNPDTGGAIAGFTRFS
                     RDTLSNNLESWVTSVQNNLCLKCHDADGAASPLARTQLGTPQRPFTSQTKDALDVSGL
                     LQSVNNFHHAVLFPGNNPYCTPTATNGNVVTMNPPWNQIANTHNRISCFDCHAVNGHG
                     GHFSGILRRETYYKDPVVNPNFANAEKNFCTICHKLTSYYLGSGGSRLNEHDKSEHRT
                     VADGGRNQYGCRGCHAGIYENDANLACDNGSARGTIHGGNFTWPICSKSPGSVSKRFM
                     VGGYLSGWQEGTVNNATCWGGGCHHSNGVRY"
     gene            complement(2426..>3358)
                     /locus_tag="AC16FU_02425"
     CDS             complement(2426..>3358)
                     /locus_tag="AC16FU_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2993260.1"
                     /translation="SCATGPDMNRYLFPTLLLLLPGQLAAQEMHLTSYSQITGWGRGA
                     SLKNPAAFWYDEFKERILVANTGANAVDVFTAEGQPLFRIGEQGELSLPVGIAASPAG
                     KIFILERNSPLLKIYDEETKKTDTFNLAGVDSTRKVSISRISADREGNLLLVDAENDR
                     ILVLDRSLRLAAKVGSSGRGEGKLQEIVALAADNSGKIFVADALNYPVQVFDRKGRFL
                     FRFEKSALAERSVWKPAGMFLDPRNRLWVIDAVAAGFRVYDPNGNLLQKVEAADGGTK
                     KFFSPIQIAADKYGLLYVLEKGKDRIDLLRIENF"
BASE COUNT          686 a          920 c         1015 g          737 t
ORIGIN      
        1 tcaaaatcga gccgggggcg gaaatcgacc cgcttggaat tgctcacgta ttcgttgcgc
       61 acgacatagg cggtaaagag gaaacgattc ttgaccatga ggtagtcctg caggttgaaa
      121 tagagaatct ggttatagag attattggaa ccaagaggca aaagttgccg ctggaaattg
      181 aactggtagt tcccgtaaat cttcgggtag gcaaaagaga cgttcctccc agccaggaac
      241 gtcacgacaa tcaagaagaa ggttttgccc accggagcca tcagtaccgc actccgttgc
      301 tgtggtggca gccgccgccc cagcaagtgg cgttattgac cgtcccctcc tgccagccgg
      361 agagatagcc gcccaccatg aaccgcttgc tcacgctgcc cggggatttg gagcagatcg
      421 gccaggtgaa attgccgccg tgaatcgtgc cgcgcgccga gccgttgtcg caggcaaggt
      481 tggcgtcatt ttcgtaaatc cccgcatggc agccccggca gccgtactga tttcggccgc
      541 cgtcagccac ggttcggtgc tcactcttat cgtgctcgtt cagacggctg ccgccggaac
      601 cgagatagta cgacgtcagc ttatgacaga tggtgcagaa gtttttctcg gcgttggcaa
      661 agttcggatt gaccacgggg tctttgtagt aggtttcgcg ccgcaggatc ccggagaagt
      721 gtcccccatg gccgttgacc gcatggcagt caaaacagga aatccggttg tgggtgttgg
      781 cgatctgatt ccagggcggg ttcatggtga cgacattgcc gttggtagcg gtcggcgtgc
      841 agtacggatt attcccgggg aataagacgg cgtgatggaa attgttgacg ctctgcagga
      901 gaccactcac atccagggca tctttcgtct gggaagtaaa gggccgctgc ggcgtaccca
      961 gctgggtacg tgccagaggt gaagccgcac cgtcggcatc gtggcatttc aggcaaagat
     1021 tgttctgcac actcgtcacc caggattcaa gattattcga aagggtgtcc cggctgaagc
     1081 gggtaaaacc ggcaatggcg ccgccggtgt cgggattccg caggtctaga accccatttt
     1141 tatggtaggt ggagttggtc cggccggtca aaggatcccc ttccatatgg cagacgccgc
     1201 agtcaaactt ggtgacgacc tctattccgg ttccgttcgt cacatggtgc gcgactttca
     1261 cgaaatcacc ggcttggccg gtgatcgctc tgcggcgggt cggcgaagcg ggatcgccct
     1321 gagccatgga atggcaggag agacaatctc ccccgggtaa ggggtggtgg acgttccgcg
     1381 tcgtgggcga agagtaatcc cggccgtggc actcgtaaca atgcgtctgg tcgttaaagg
     1441 cgtgctcggc gacactactg ggcaaggcgg ctaaggtcac tccttccacc gttccgcccc
     1501 cctgcccgtc cgaggatttg tggcagccga aacagaagcg tctgacctgg gtgcttgaag
     1561 tttgcaggga atccaggccg gaccagccgg ccctttggtc actcaagagg aaggtattcg
     1621 ggtgcaggcc gtcgttcccc tgcgacccgt ggggattgtg acagtcgtag cagggcagtt
     1681 tgtcgccggc ccggacgtag ccgaaggtgg tcttgaactg gtgcccggac tcgccgtcgt
     1741 tattgaccga tttgtcgtag taatccgcaa tccgtttatt ttccggattc ataccggccg
     1801 ggccgtccgt ggaatggcat ttgaaacaga gcgcgtaatt ccccggacgc ccggagacga
     1861 aagccccctg gatgttcagg taagttgtgt ccagcatgtc aaagggattg cgggtgccgt
     1921 ggggattatg gcagttcagg cacatgcccg agccgttatt atccttgcgg ggcatgtcga
     1981 gatccgaacg gtggggggag aaatattttc ctgaggccga actgccgctg ttctcgtaga
     2041 ccagttgtcc gggccaccgt ttgcggtttt ccactccggt gattttgacc ccgttggagt
     2101 tgtattcaaa gtagccggga taggtgctgg cgatcggcag gcggtcgctt tcctgcgccg
     2161 gatagccgat cggcttctgg gagtggcagg tgtagcagag gttgttgttg ttttcggtga
     2221 agagcaaaag tgcttccggt gttatatcct tgtgcgccag gtggcactgg ctgcactctc
     2281 cccgggcaaa gcggttatcc cgcagaacgc ccgtgttcgg atcgccgtgc ggcgaggtgt
     2341 cgtaatttcc cgtctgcgcc cgaaccaaaa gcgcggccac gaagccgagg agcgaaccga
     2401 tggcccaagc ggaacgtttc ataagctaaa agttttcaat cctgagcagg tcaatccggt
     2461 cttttccttt ttccagaaca tacagcaacc cgtatttatc cgccgctatt tgtatcggcg
     2521 agaaaaattt tttcgttcct ccgtcggcag cctcaacctt ctgcaagagg ttgccgttgg
     2581 gatcatacac gcgaaaaccc gccgccaccg catcgatgac ccagaggcgg ttccgggggt
     2641 ccaagaacat cccggccggt ttccagacgc tccgttccgc gagggccgat ttttcaaagc
     2701 ggaacaaaaa ccttcctttc cggtcaaaga cctgtaccgg ataattcaga gcgtcggcca
     2761 caaagatttt gccggaattg tccgccgcca aggccacgat ctcctggagt ttcccctccc
     2821 cgcgcccgga ggagcccacc ttggcggcga gccgcaagga cctatccaga accagaattc
     2881 ggtcattttc cgcatcgacc agaaggaggt tcccctcccg atcggcggaa atccggctga
     2941 tggaaacctt ccgggtggaa tctaccccgg ccagattgaa ggtgtcagtc ttttttgtct
     3001 cctcgtcata aattttcaag agcggactgt tccgctccag aataaagatt ttgccggcag
     3061 ggcttgcggc aatgccgacc gggagggaga gttccccttg ttcaccgatg cgaaagaggg
     3121 gctggccctc cgccgtgaaa acgtccactg cattggcgcc ggtgttggcg accaaaatcc
     3181 gctctttgaa ctcatcgtac cagaacgcgg ccggattttt cagagatgct cctctccccc
     3241 agccagtaat ctggctgtac gaggttaagt gcatttcctg tgcggcaagc tggccgggca
     3301 ggagcaggag gagagtcggg aagagatacc tgttcatatc aggtccggtg gcacagga
//
LOCUS       DBNGZA010000111         4201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2459926, whole genome shotgun sequence.
ACCESSION   DBNGZA010000111 DBNGZA010000000
VERSION     DBNGZA010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2459926"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC16FU_02430"
     CDS             complement(<1..765)
                     /locus_tag="AC16FU_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2993261.1"
                     /translation="MLDFFAIAALCLVWGGTWLAIKIGLSDAPPFLSAAARFLIACLL
                     LFPFIWRKKLALPKTKKEISAILVTGFCMYALSYALVYWAEQYVPSGLTAIIFSTLPF
                     LVALFAHFLLPAEPFTLPKTVGMVVGFAGIGLIFWEGAGIAGSRQFLGMLLLLGSPLA
                     TSYANVLTKRDLHDVHPAVIAGLQMALGFVLLSVLGFAFERFADFHLTHRSLGSLAYL
                     ATFGSVFAFATYYWLLKRVEATKLSLIAFVTPVVALI"
     gene            complement(778..1245)
                     /locus_tag="AC16FU_02435"
     CDS             complement(778..1245)
                     /locus_tag="AC16FU_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993262.1"
                     /translation="MQQIDVKDTVLIVVGVELNAEEKDRPIAYYLKSQIDKHARAAGK
                     PYRKGIVITDALWLRDKIVQICPTIALGGPGVNALAAQLVDKLPVAYGEENRFFIQME
                     EQVLNNKISIWGMNQATTKMGVDTFLSRGLLDKYLEVVWPSGSTRVESDPSGK"
     gene            1662..3368
                     /locus_tag="AC16FU_02440"
     CDS             1662..3368
                     /locus_tag="AC16FU_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2993263.1"
                     /translation="MRSGASVSGTAMRWCGLFLIFFLAGNLAAQTGRKVKMEPGGSLR
                     LFLSAERVELAALAESVSFPVNTLQADNLTIRSGQRVVADKDGFHMNVQIFAAGQVSE
                     IVVGKKKDSVVTKHPSEDDTFSLFSAFSVGPDDFLRGDLFLFGGSAAVAGEISGDIIA
                     LFGSVRLEPTAVVRGDVVAIGGSVEVAEGAMTYGEITTVRSSDSKVSTISHRKRGIRF
                     RFDSRFDPGFTYNRVDGVALFPRYTFQGKPNGRPTVHLAGGRAFERNRYLYDFGFKEE
                     IGDSNTFLFGGSFYRYTATDDDWILPAGENNVSTLFVRRDFRDYVEREGINGFVGQKL
                     FRYHTVRLDYFFDRYRPLRNASNWSLFGGPKRFRANFSRVPDTLLVRYAGDFNAEIGS
                     AIFTYAFDTRDDEDDPTRGWLGKLEIEHGGPDLGGDLFYTRWRAVLQRYQPITLDQNL
                     SLRAMYGNSGERLPLQRLFFLGGLGTMRGYEFKEFSGDQFALLNADYDVRLPKGSLTA
                     VLFFDLGTATFRRNLFDAGPWKADVGLGLKLGSVVRFDLAKRLDDFDRNLKLTVRFAR
                     MF"
     gene            complement(3482..3835)
                     /locus_tag="AC16FU_02445"
     CDS             complement(3482..3835)
                     /locus_tag="AC16FU_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR2993264.1"
                     /translation="MKKFVEIRSLNLKPGTRGEFHRRYLEKALPLLNKWKMNVVAFGP
                     SPHDENSYYVIRAFESLEHRQESEETYYGSSDWRQGPREEMLALIENYIDIVIEMEEE
                     TVNALRESSTPYSLP"
     gene            complement(3904..>4201)
                     /locus_tag="AC16FU_02450"
     CDS             complement(3904..>4201)
                     /locus_tag="AC16FU_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HIR2993265.1"
                     /translation="TRQPTADSAGQTKISRMAFDKKFSGDLQATSKGEMLAAGTGAPG
                     SSGGYVAIKQVSGSLNGKKGSFILQHSGTMRRGAPELVITVVPDSGADELKGLA"
BASE COUNT          856 a         1170 c         1287 g          888 t
ORIGIN      
        1 gatcaacgcc accacgggtg tcacaaaagc aatgagtgag agtttggttg cttccacccg
       61 cttcaggagc cagtagtagg tcgcaaaggc gaagaccgaa ccaaaggtcg ccaggtaggc
      121 cagggagccg agtgaccggt gggtgagatg gaaatcggcg aaacgttcaa acgcgaaacc
      181 cagaacggaa agcaaaacga atcccagagc catctggagt ccggcgatca ctgccggatg
      241 tacgtcgtgc aggtcgcgct tggtcaggac gttggcgtag gaggtcgcta aagggctgcc
      301 gaggaggagg agcatcccca gaaactgccg ggagccggcg atcccggctc cttcccagaa
      361 gatgagtccg atgcccgcaa aaccgacgac catgccgacc gttttgggca gggtgaacgg
      421 ctcggcgggc aaaaggaagt gggcgaagag cgccaccaag aaaggcaaag tcgagaagat
      481 gatcgcggtc agcccggagg ggacgtactg ctcggcccag tagaccaatg cgtaggagag
      541 ggcgtacatg caaaagccgg tgaccagaat ggcggagatt tcctttttcg ttttcgggag
      601 ggccagtttt ttgcgccaga tgaaagggaa gaggaggagg caggcaatca aaaaacgcgc
      661 cgccgcgctg agaaaaggcg gcgcatcgga taagccgatt tttatcgcca gccaggtgcc
      721 gccccagacc aaacagagtg cggcaatggc gaagaagtcg agcattgagg gaggagacta
      781 cttgcccgac gggtccgatt cgactcgtgt cgagccggag ggccagacta cttcgaggta
      841 cttgtccaga agcccccggg agaggaaggt atcgaccccc attttggtcg tggcctgatt
      901 catcccccag atggaaattt tgttgttcag gacctgctct tccatctgga tgaagaagcg
      961 gttctcttcg ccgtaggcca ccggcagttt gtccaccaac tgtgcggcca gggcgttcac
     1021 ccccggaccg cccaaggcga tggtcgggca aatctgaacg attttgtccc gcagccagag
     1081 ggcgtcggtg atgacgatgc ccttgcggta cggtttgccc gccgcgcgcg catgcttgtc
     1141 gatctggctt ttgaggtagt aggcaatggg ccggtccttc tcttcggcgt tcagttcgac
     1201 ccccaccacg atcagaacgg tatccttgac atcaatctgc tgcacgggga actcccagcg
     1261 aatattttct catagacaaa tccagacagg caaataagga ttttccgaga ggatgtcaaa
     1321 caacaggacg gatttgggat gagggggtta ggatttgggg aaatatgaga ggggagttcg
     1381 acccggatag aaagggtaac aaaacggagg agaaattccg gccacgggaa ttcggtcagg
     1441 ctggtcagac acgactctac cgagaagcct gacctatgga tgagagacat ttctacctcc
     1501 atagccgagg tttctagcct cggaacggcc acaagggcct atgcctatgc gtggaggtag
     1561 tagatcaatg caccttagcc aggagggcat aactcagaac gcgcacttgg acttcaggga
     1621 agggctcttg tattatatac cgagcgagct atggagggcc ggtgagaagc ggagcatctg
     1681 ttagcggcac agccatgcgc tggtgtggat tgtttttgat tttttttctg gcgggcaacc
     1741 tcgccgccca gaccgggcgg aaagtgaaaa tggagcccgg gggatctctg cgtctttttc
     1801 tttcggcgga gcgggtggaa ctcgccgcgc tggcggagag cgtctctttt ccggttaata
     1861 ccctgcaggc cgataacctg acgatccgct ccggtcagag agtggtagcc gacaaggacg
     1921 gctttcacat gaatgtgcag atattcgcgg cggggcaggt gtcggaaatc gtcgtgggga
     1981 agaaaaaaga ttccgtcgtc accaaacatc cgtcggaaga cgacaccttt tcgctcttca
     2041 gcgcgttctc cgtcggcccg gacgactttt tgcgcggcga tctcttcctc ttcggcggga
     2101 gtgccgccgt cgcgggggag atctccggcg acatcatcgc gctgttcggc tcggtacggc
     2161 tggaaccgac ggcggtcgtc cggggtgacg tggtggctat cgggggcagt gtggaggtgg
     2221 ccgagggtgc catgacctac ggggaaatca cgacggtgcg ctcctccgac agcaaagtct
     2281 ccaccatcag ccaccgcaag agaggcatac gcttccggtt cgactcgcgt tttgatccgg
     2341 gctttaccta caaccgggtc gacggtgtgg cgcttttccc gcgctacacc ttccagggca
     2401 agccgaacgg gcgccccacc gtccacctgg ccgggggccg ggcctttgag cgcaaccggt
     2461 atctgtacga tttcgggttc aaagaggaaa ttggcgacag caataccttc cttttcggcg
     2521 ggagtttcta ccgctacacc gccaccgatg acgactggat tctgccggcg ggggagaata
     2581 acgtctccac gctctttgtg cggcgcgact tccgggatta tgtcgagcga gaggggatca
     2641 acggttttgt cggccagaaa ttattccgct atcacaccgt ccgcttagac tacttcttcg
     2701 accggtaccg tcccttgcgg aacgcgagca actggagtct cttcggcggg cccaagcggt
     2761 tccgcgccaa cttttcgcgc gttcccgata cacttctggt gcgctacgcc ggggatttca
     2821 acgctgaaat tggctcagcc attttcacct acgctttcga tacgcgtgac gatgaagacg
     2881 atcccacccg gggttggctg ggcaaattag aaatcgagca cggcggcccg gatctcgggg
     2941 gcgatttgtt ctacacccgc tggcgcgccg tcctgcagcg gtaccagccg ataaccttgg
     3001 atcagaacct gtcgctgcgg gccatgtacg gcaattcggg cgagcggctc ccgttgcagc
     3061 gcttgttctt cctcggcggc ctcgggacta tgcgcggcta cgagttcaag gaattttcag
     3121 gcgaccagtt cgcccttttg aacgccgact acgacgtgcg gctgcccaaa gggtctttga
     3181 ccgccgtcct gttcttcgat ctgggcaccg ccaccttccg gcggaatctg ttcgacgccg
     3241 ggccgtggaa agccgacgtg ggcctcggcc tcaaacttgg cagcgtggtc aggttcgatc
     3301 tggccaagcg gctggatgac ttcgaccgga atttgaaact gacggtgcgg ttcgcgcgga
     3361 tgttttaagt ttgaggggga cgggggcgcg gcctcgtgcc ccctgaacta aggggaaggc
     3421 tgaccccctc cggcactccc tcgacttcgc tcgggacaag ccgtgcctcg tccccctgtt
     3481 tttaagggag actataaggg gtagaacttt cccgcagagc gttgacggtt tcctcttcca
     3541 tttcgatgac gatgtcgatg tagttttcta tcagcgccaa catctcctcc ctcggcccct
     3601 gccgccaatc ggagctcccg tagtaggttt cctcgctctc ttgacggtgt tccaaacttt
     3661 caaaggcgcg gatgacatag taggaatttt catcatgagg ggaggggccg aaagcgacga
     3721 cgttcatttt ccatttgttt agcaagggta gcgctttttc caagtagcgg cggtgaaact
     3781 ctccgcgggt gcctggcttt aaatttaaag agcggatttc aacgaatttt ttcatcccta
     3841 ctccgggagg gtgtactcga agacgtagga atgtttcccc tcttcgattt tgatttccat
     3901 ttttcacgcc agacccttca actcgtctgc ccccgagtcc ggcaccacgg tgatgaccag
     3961 ttccggcgcg ccgcgcctca tcgtgccgct gtgctggagg atgaaacttc cctttttgcc
     4021 gttcaaactc ccgctcacct gcttgatggc aacgtaaccg ccggaggagc ccggagcgcc
     4081 cgtgccagcc gcgagcatct cccccttgct ggttgcttgg aggtcgccgg aaaacttttt
     4141 gtcgaaggcc atccggctga ttttcgtctg cccggcgctg tcggccgtcg gctggcgagt
     4201 t
//
LOCUS       DBNGZA010000112         2059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1187400, whole genome shotgun sequence.
ACCESSION   DBNGZA010000112 DBNGZA010000000
VERSION     DBNGZA010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1187400"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /gene="uvrA"
                     /locus_tag="AC16FU_02455"
     CDS             complement(<1..1921)
                     /gene="uvrA"
                     /locus_tag="AC16FU_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR2993266.1"
                     /translation="MSQSVIRIKGAREHNLKNIDVEIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIDGLSPAISIEQRSAAKNPRSTVGT
                     VTETYDYLRLLYARIGVPHCYRCGRKISQQATDQIVDAVLDLPRSTKIQILAPLVSGR
                     KGEHKEVFDEAKRKCFVRVRVDGKVLELGPEIKLEKKKKHSIEVVVDRLVVEPKIKKR
                     LADSLETALSTGGGLVMVERVGSSTATPIKSRSAKGAKPTELLQFSQHFACPHCGISY
                     EEPAPRLFSFNSPFGACPTCDGLGTKMEIDPDRIIPDPNLSINQGAIAPWGDAIGSWY
                     RQMLTSVADHYRFSLHTSWSRFSEEARHIVLYGTGKKELRFTYEHREGKGHGEYTGQF
                     EGVIPNLERRYRKTESAGVREWIEKFMAVRPCPTCNGARLKKEALAVKLNGLSISELT
                     AFTVKKTREFFKDLTLTKREQVIAKQILKEINERLGFLVNVGLDYLTLDRSATSLSGG
                     EAQRIHLATQIGSRLMGVLYILDEPSIGLHQRDNKRLLNTLIGLRDLGNTVVVVEHDR
                     ETIQTADFVVDLGPGAGKNGGEVVVSGPPEVVARTPRSITGQYLSGKRFIPIPERRRN
                     GSGKYVKLIGATGNNL"
BASE COUNT          393 a          595 c          609 g          462 t
ORIGIN      
        1 tcaaattgtt gccggtggcg ccgattaact tcacatactt gccgctgccg ttgcgccgtc
       61 tttccggaat cggaatgaaa cgtttgccgg ataaatactg gccggtgatg gaacgcgggg
      121 tgcgcgcaac cacttccggg gggccggaaa ccaccacttc gccgccgttt ttcccggccc
      181 ccgggcccag gtcaaccaca aaatcggcgg tctgaatcgt ctcgcggtcg tgctccacga
      241 cgacgacggt gttgcccaaa tctcgtaggc cgatgagcgt gttcaaaagg cgtttattat
      301 cccgctggtg caggccgatg gacggttcat ccagaatgta aaggacgccc atcaagcggg
      361 agccgatctg ggtggccagg tgaattcttt gggcttcgcc gccggagagc gaggtagccg
      421 agcggtccag ggtgaggtag tcgaggccca cattgaccag aaaccccagc cgctcgttga
      481 tttctttcaa gatctgtttg gcaatcacct gttcgcgctt agtcaaagtc aaatctttga
      541 agaactctcg ggttttcttg accgtgaagg cggtcaattc tgatatggaa agtccattca
      601 gtttcaccgc cagggcttct tttttcaagc gcgcgccgtt gcaggtgggg cagggccgaa
      661 ccgccatgaa cttctcaatc cactcccgca cccctgccga ttcggttttc cggtagcgcc
      721 gctccaaatt ggggatgacc ccttcgaact ggccggtgta ctcgccatgc cccttccctt
      781 cccggtgttc gtaggtgaag cgcagttcct ttttgccggt gccgtacagg acgatatgcc
      841 gggcctcttc gctgaagcgg ctccaggatg tgtgcaggga gaagcggtag tggtcggcca
      901 cgctcgtaag catttgccgg taccagctgc cgatggcgtc cccccagggt gcgatggcac
      961 cctggttgat ggaaaggttc gggtcgggaa tgatgcgatc gggatcgatc tccatcttgg
     1021 tgcccaggcc gtcgcaggtg gggcaggcac cgaaaggcga gttgaacgaa aagaggcgcg
     1081 gcgccggctc ctcgtacgag atgccgcagt gggggcaggc gaagtgctgg gagaactgca
     1141 aaagctcggt cggcttggca cctttcgcgc tccgcgattt gatcggggtt gcagtggacg
     1201 aaccgacgcg ttccaccatc accagcccgc cgccggtgga gagtgcggtc tccagcgaat
     1261 cggccaggcg ctttttgatt ttgggctcca ctaccaggcg gtccactacc acttcgatac
     1321 tgtgcttctt tttcttctcg agtttgattt ccggccccaa ctccagcact ttgccgtcca
     1381 cccgcacccg gacgaagcac ttccgctttg cctcgtcgaa gacctcttta tgctcgccct
     1441 tccgcccact caccagaggc gctaagattt gaattttcgt gctccggggc agatcgagca
     1501 ccgcatcgac gatctggtcg gtcgcctgct ggctgatttt cctcccgcac cggtagcagt
     1561 ggggcacgcc gatccgggcg tacaaaagtc tcaggtagtc gtaggtttcc gttacggttc
     1621 ccacggtgga gcgggggttt ttggcggcgg agcgctgctc gatggagatg gcgggcgaaa
     1681 gcccgtcaat gaaatcgacg tccggtttct ccatcaggcc cagaaactgc cgggcgtagg
     1741 ccgaaagcga ctccacgtag cggcgctggc cttcagcgta aatggtgtcg aaggccaaag
     1801 acgacttccc cgatccggac aggccggtga tcaccaccag cttatcgcgg ggaatctcta
     1861 catcgatatt ttttaggtta tgctcgcggg cgcctttgat gcgaataacc gactgcgaca
     1921 aagcgaaacc tccattttgg gcaaccggtg aatatacaac ccaaggggtg gaggttcaat
     1981 cagatttttc agaaaaagtt cttaaccttt gtcctacgac cttcgctgag tgctatctgc
     2041 ctttttccaa ggaggggaa
//
LOCUS       DBNGZA010000113         4699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1717711, whole genome shotgun sequence.
ACCESSION   DBNGZA010000113 DBNGZA010000000
VERSION     DBNGZA010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1717711"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="AC16FU_02460"
     CDS             <1..1008
                     /locus_tag="AC16FU_02460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR2993267.1"
                     /translation="FGCEHVNVQPHSGSQANMGAYLSVLDIGDTIMGLNLSHGGHLTH
                     GHPINFSGRFFIVVAYGVSKETETIDYDEVRRLAREHKPKMIVVGASAYSRIFHFPKF
                     REAADEIGAFLMVDIAHIAGLIVAGLHPSPVPYADFVTTTTHKTLRGPRGGMIMCRAK
                     YAKDLDRMIMPGIQGGPLMHVIAAKAVAFKEALTPEFKAYQVQIVKNARTLAEELVRL
                     GYHVVSGGTDTHLMLVSFIEKKLTGKMVEKALERAGITVNKNTVPFDPQQPFVTSGIR
                     IGTPAVTTRGMKEPEMKAIAGLIHRVISNLGDEDTIRQVKAEVTRLCDRFPLYQERLV
                     G"
     gene            1009..1365
                     /gene="cutA"
                     /locus_tag="AC16FU_02465"
     CDS             1009..1365
                     /gene="cutA"
                     /locus_tag="AC16FU_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR2993268.1"
                     /translation="MARRSAVRIDPHKFVLVLSTTAKQRVAERIARALVREKLAACVN
                     VIPGLISHYRWKGRVERSHEWLLLVKTRAALFPRLAERVKELHPYAVPEIISVPLAAG
                     SNDYLRWILEATMKER"
     gene            1365..1787
                     /locus_tag="AC16FU_02470"
     CDS             1365..1787
                     /locus_tag="AC16FU_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR2993269.1"
                     /translation="MQFQRIPSGYLVRLVKGEEIITTLAGFVEREKVKGGGFTGLGAM
                     ADVELGYFDAHTREYVRKEYPGEYEIGNLTGNIAWLDGKPVVHAHVTVSGPDLAAHTG
                     HLFKGIVSVTCEITLVAAEATIRRALDPDIGLNLLALE"
     gene            1784..2623
                     /locus_tag="AC16FU_02475"
     CDS             1784..2623
                     /locus_tag="AC16FU_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR2993270.1"
                     /translation="MTARHESHSTGPLVTFTSDFGSADAYVPAVKGVIASINPRARII
                     DTIHQIPPHNIVRGALMLKGFVPYYPPGTIHLAVVDPGVGSKRRALLIVTDHGFFVGP
                     DNGLFALVVPASGHKKVIHLKEKRFWLNPQSPVFHARDIFAPVAAYLSRGVKPEEFGP
                     EVKGIKSLAIPKPHRSAAAIRGDVISVDRFGNLTTNIPGEWLLQKVAGSREPVARRQF
                     KIHSGPHELAGLFETYALIPPKKAGAVIGSAGFLEVALNRASAAERLQANVGTLVAIR
                     LSG"
     gene            2719..3171
                     /gene="nrdR"
                     /locus_tag="AC16FU_02480"
     CDS             2719..3171
                     /gene="nrdR"
                     /locus_tag="AC16FU_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR2993271.1"
                     /translation="MKCPYCGHEEDKVVDSRTAQDGRAVRRRRECLACGERYTTYEYV
                     ENRELVLVKNDGRREPFDRQKLRRGIELACNKRPVSAKKIDAVVDEIEEELQNLSKGE
                     ISSQEVGEKVMEKLKGLDDIAYVRFASVYRKFQDIEHFQEELNKLLNK"
     gene            3300..4040
                     /gene="tatC"
                     /locus_tag="AC16FU_02485"
     CDS             3300..4040
                     /gene="tatC"
                     /locus_tag="AC16FU_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR2993272.1"
                     /translation="MPRDSKAMPFLAHLEELRWRIIKCLASILVGSVVIYFFSDQMLD
                     FLTRPVRAVYFSGVTEAFAVRIKLSLYGGLILSVPVILYQAWQFVVPGLLEREVKVAL
                     PVVVAATLFFAGGGAFCFFVVLPAGIKFLLTFGSAKLQPLITVDKYVSFVSFMVIAFG
                     AVFELPVLAFVLGQLGIISHRTLAKGRRYAVVLIVILAAILTPTPDAFSQLMLAVPLY
                     FLYELSILVVRLTGRRKLGNDVRVLTPN"
     gene            4120..>4699
                     /locus_tag="AC16FU_02490"
     CDS             4120..>4699
                     /locus_tag="AC16FU_02490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR2993273.1"
                     /translation="MTKRLSSALVMAVLTHLLLAAVVWAQAPPAPSALPDYLRSAGMV
                     EKPVEVVTDSVRLPALLTLPDSTGKGFPIVVLVHGSGPNDKDETVGPNKPFRDLAYGL
                     AKQGIATLRYDKRTRAGYTQVKLLEDEVILDALSALRLAKSFPQVDSAKVFLLGHSLG
                     ATVAPYIARRAPGLAGVIALAGLTQPWDATLLR"
BASE COUNT          947 a         1341 c         1407 g         1004 t
ORIGIN      
        1 ttcggctgtg agcacgtcaa cgtccagccc cactccggct cgcaggccaa catgggtgcc
       61 tacctctcgg ttctggacat tggcgacacc atcatggggt tgaacctgtc ccacggcggc
      121 catctcactc acggccatcc gatcaatttc tccggacggt tcttcatagt cgtcgcgtat
      181 ggagtttcta aagaaacgga aaccatcgat tacgatgaag tcaggcggct cgctcgcgaa
      241 cataagccga aaatgatcgt cgtcggggct tcggcctatt cgcgtatttt ccacttccca
      301 aaattccgcg aggcggcgga tgaaatcggc gcgttcctta tggttgacat tgcccacatc
      361 gcgggcttaa tcgtcgccgg attgcatccg tcgccggtgc cctacgcgga ttttgtcacc
      421 accaccacgc acaaaaccct gcgcgggccg cgggggggaa tgatcatgtg ccgggcgaaa
      481 tatgccaaag atctcgaccg gatgattatg cccggtattc agggcgggcc tttgatgcac
      541 gtcattgcgg caaaggcggt ggcgttcaaa gaggctctca ccccggagtt caaggcctac
      601 caggtacaaa tcgtcaaaaa tgccagaacg ctggccgagg aattagtgcg gcttgggtac
      661 cacgtcgtct ccggcggcac cgacacccac ttgatgctcg tcagtttcat tgagaaaaaa
      721 ttgacgggaa agatggtgga gaaggcgctg gaaagggcgg gcattacggt gaacaagaac
      781 accgtcccct tcgaccctca gcagccgttc gtcacctccg gcatccgcat cggcacgccg
      841 gcggtcacca cccgggggat gaaagagccg gagatgaaag ccatcgccgg cctgatccac
      901 cgggtgattt ccaatctggg ggacgaggat accatccggc aggtgaaggc cgaagtcacc
      961 cgcctgtgtg accgtttccc gctctatcag gaacgcttag ttgggtaaat ggcccgccgg
     1021 tccgccgtcc gcattgatcc gcataaattc gtcttagttt tgagcaccac cgctaagcag
     1081 cgcgtggccg aacggattgc cagagcgtta gtgcgcgaaa aactggcggc ctgcgtgaac
     1141 gtcattccag gtttgatttc ccactaccgc tggaaaggga gagtggagcg atcacacgaa
     1201 tggctgcttc tggtgaaaac ccgggcggcg cttttccccc ggctggcgga gcgggtgaag
     1261 gaacttcacc cctatgcagt gcctgaaatc ataagcgttc ctcttgcggc gggaagtaac
     1321 gactacctgc gctggatttt ggaagcgacg atgaaggagc ggtaatgcaa tttcagcgaa
     1381 ttccttccgg ttacttggtg cggctggtga agggggagga gatcatcact acgctcgccg
     1441 gatttgtcga gcgggaaaaa gtcaaaggtg gcggtttcac cggcttgggg gcaatggctg
     1501 acgtggagtt gggctacttc gacgcgcaca cacgggagta cgtgcgcaag gagtacccgg
     1561 gggagtatga gatcgggaac ctcaccggca acatcgcctg gctggacgga aagccggtgg
     1621 tgcatgccca cgtcaccgtc tccggcccgg atctggccgc gcacaccggg caccttttta
     1681 agggcatcgt ctcagtcacc tgcgagatca ccctcgtggc ggccgaggcc accatccggc
     1741 gggcgctgga tcccgatatt gggttgaatc ttctggccct ggaatgaccg ctcggcacga
     1801 gtcgcattcg accgggcctt tggtcacctt cacgagcgat ttcggctcag ccgatgccta
     1861 cgtaccggca gtcaagggcg ttatcgcttc catcaatccg agagcccgta tcatcgatac
     1921 tatccaccag ataccacccc acaatattgt gaggggtgcg ctgatgctca aaggttttgt
     1981 gccatactat ccgccgggaa cgattcatct tgccgtggtt gatccgggag ttggctcaaa
     2041 gcggcgcgcc cttttgattg tcaccgacca cggctttttc gttgggccgg ataacggcct
     2101 ttttgcgctc gtcgtccccg cctccggcca caagaaagtc attcatctca aagagaaacg
     2161 gttctggctc aatccgcagt caccggtctt tcacgcgcgg gatatttttg cgccggtggc
     2221 ggcctacctc tcgcgcgggg tcaagccgga agaattcggg ccggaggtca agggaataaa
     2281 atctctggcg attcccaaac cgcaccggag tgctgctgct attcgcggcg atgtcatctc
     2341 tgttgataga ttcggaaatc tcacgacgaa catcccaggg gaatggctcc tgcaaaaagt
     2401 agccggtagc cgggagccag tagccagaag acaattcaaa attcactccg ggccgcatga
     2461 actcgcggga ctttttgaaa cctatgcact gattcctcca aagaaggcgg gagcggtaat
     2521 cgggagtgcg ggatttctcg aagtggcgct gaaccgggcg agcgcggctg aaagactgca
     2581 ggcaaacgtc ggcactctcg tcgcaatacg cctgtccgga tgactccaat tccgggtaga
     2641 tggggtgtct ccccctcgtg catcttctcc aaagtttggg tagacggggc gtcccgcccc
     2701 gtgtattttg ccctctggat gaagtgcccc tattgtggtc acgaagagga taaggtggtc
     2761 gattcccgca ccgcgcagga tggccgcgcg gtcaggcggc ggcgcgagtg cctcgcctgc
     2821 ggcgagcgct acaccaccta cgagtacgtc gagaaccggg aactggtttt agtgaaaaac
     2881 gacggacggc gcgagccgtt cgaccgccag aaactgcgcc gggggatcga actggcctgc
     2941 aacaaaagac cggtgagcgc caaaaaaatc gacgcggtgg tggatgaaat cgaggaagaa
     3001 ctgcagaatc tctccaaagg agaaatctca agccaggaag tgggggagaa ggtgatggaa
     3061 aagttaaagg ggctggacga cattgcctac gtccgcttcg cttcggtcta ccggaagttt
     3121 caggacatcg agcatttcca ggaggagttg aacaaactct tgaacaaatg agagaaacgg
     3181 agtcagggat taggatttgg gagttgggga aaggattggt aacctaggcc ctggtggccg
     3241 ttctgatcaa tccgacggct caaaatctcc gccccgtcgg tgaaagcccg gcgccccgta
     3301 tgccgcgtga ttccaaagca atgccctttc tggcgcacct ggaggaactt cgctggcgca
     3361 tcatcaagtg cctggccagc atcctggtgg gctcagttgt catctacttt ttctccgatc
     3421 aaatgctgga tttcctcacc cggcccgtac gggcggtcta tttttcgggc gtgaccgaag
     3481 cctttgcggt gcgaatcaaa ctgtcgctct acggcgggct gattttaagc gtgccggtga
     3541 tcctctacca ggcctggcag ttcgtcgtcc ccggccttct ggagcgcgaa gtgaaggtgg
     3601 cgctgccggt ggtcgtcgcc gccacgttgt ttttcgctgg gggcggcgcc ttctgcttct
     3661 tcgtggtgct gccggcgggg atcaaattcc tcctgacatt tggctcggcc aaactccagc
     3721 ccttgatcac cgtcgataag tacgtcagtt tcgtctcgtt catggtgatc gccttcggcg
     3781 cggtgtttga actgcctgtt ctggccttcg ttctggggca attggggatc atctcccacc
     3841 ggacgctcgc gaaagggcgg cgttacgctg tcgtcctcat cgtcatcctg gcggcgattc
     3901 tcacgcccac gccggatgcc ttttcgcagc tgatgctggc cgtgccgctc tattttctct
     3961 acgagctgag catactggtg gtccggttga ccggccgaag gaagttgggt aatgacgtac
     4021 gagttttgac acccaattga gtcttcattc ccccacacaa gacttttccc tgtcgatacc
     4081 tttgggattg actatattcg ggacaacaag gagagtagca tgaccaaacg tctttccagt
     4141 gctctggtaa tggcggttct gactcatctg ctgctggcag cggtggtttg ggcccaggct
     4201 cccccggcgc cttcggcgct gcccgactac ctccgctccg ccggtatggt cgaaaagccg
     4261 gtggaggtgg tgaccgactc ggtgcgcttg cccgccctcc tcactttgcc ggacagcacg
     4321 ggcaaaggat ttccgatcgt tgtgctcgtt cacggctcag gcccgaatga taaggacgaa
     4381 acggtggggc ccaataaacc gttccgcgac ttggcctacg gtctggccaa gcaggggatt
     4441 gccacgctcc gctacgacaa aaggacgcgc gcggggtata cgcaggtcaa acttctcgaa
     4501 gatgaggtta ttctggatgc gctttcagcc ctgcgcctgg ccaaaagctt tccgcaggtt
     4561 gactcggcca aagttttcct gctgggccac agtctgggcg ccaccgtggc accctacatt
     4621 gcccgacgtg cgccgggatt ggccggggtg atagcactgg cgggtttgac ccagccgtgg
     4681 gatgccaccc ttctgcggc
//
LOCUS       DBNGZA010000114         2680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_976024, whole genome shotgun sequence.
ACCESSION   DBNGZA010000114 DBNGZA010000000
VERSION     DBNGZA010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_976024"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="AC16FU_02495"
     CDS             <1..434
                     /locus_tag="AC16FU_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993274.1"
                     /translation="FFWYYLGKRNALAFYYWRREMHLIEEEFQKIGQGYEVYNIFSNR
                     DRYYAGEPVATLSSDLVESRGESVRTDKIHQYIPGDGSRRPQVRLYAGQGIERQFPWR
                     FLGNSLNFQMIYTPAFFLVAWAVVLGMSIFFPHFFLSGRVR"
     gene            438..995
                     /gene="thpR"
                     /locus_tag="AC16FU_02500"
     CDS             438..995
                     /gene="thpR"
                     /locus_tag="AC16FU_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR2993275.1"
                     /translation="MRTFIAVELPEEVKTRVAEVSAGLSKTGADVKWVELQNLHLTLK
                     FLGEVGQEKIEAVFEKTQHAAAGTKAFQLALESTGCFPNPKRPRVIWIGLSAGKTELV
                     QLAARLENEMASLGFPREDRPFAGHLTIGRVRSPKNLDRLLAAMNGTAFSHSGVTVDH
                     VVVMKSDLTPRGPVYSRLKTITLEP"
     gene            1004..2071
                     /gene="recA"
                     /locus_tag="AC16FU_02505"
     CDS             1004..2071
                     /gene="recA"
                     /locus_tag="AC16FU_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR2993276.1"
                     /translation="MPVEIAQEKRRALDQAVTQIERQFGKGAIMRMGEDHKVAVPVIS
                     TGSVALDAALGVGGVPRGRVVEIFGPESSGKTTLALHIIAEAQKAGGVAAFIDAEHAL
                     DTTYAKHLGVDLDNLLISQPDTGEQALEITETLVRSGAVDLVVIDSVAALVPKAEIEG
                     EMGDAHMGLQARLMSQALRKLTGTISKSKSCVLFTNQIRMKIGIMFGNPETTTGGNAL
                     KFYASVRLDIRRISTIKEGEQVVGSRTRVRVVKNKLAPPFKEAEFDITYGIGISREGE
                     LVDIGSDLKILEKSGTWYSFNGEHLGQGRDNTRQYLKDYPDVANKIAAKIRERLGLVK
                     VESASGGKEQPEKKEPAKAKK"
     gene            2151..>2680
                     /locus_tag="AC16FU_02510"
     CDS             2151..>2680
                     /locus_tag="AC16FU_02510"
                     /inference="COORDINATES: protein motif:HMM:NF047066.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR2993277.1"
                     /translation="MGQGVITKIARQKRARERVSLYVDGRFLCGLDEVAFHELHLKEG
                     DRLSSYSQQELKAQSVAGKAKDAALRLLAIRPRSRKELARRLQKKGYGAAEIESVLHQ
                     LQEENLLDDERFARLYAEHRLTSRHIGQRMLLAELLQKGINRTVAQSVVNEITGDDRE
                     KIEARVFAEKKLRLLS"
BASE COUNT          608 a          726 c          826 g          520 t
ORIGIN      
        1 acttcttctg gtactatctg ggcaagcgta acgccctggc tttctactac tggcggcggg
       61 agatgcattt gattgaggag gagttccaga agatcggcca ggggtacgag gtctacaaca
      121 tattttccaa ccgcgaccgc tactatgccg gagagccggt ggctaccctc tcttccgatc
      181 tggtggagag ccggggggag agcgtccgga ccgataagat tcaccagtat attccgggcg
      241 acggcagccg gcggccccag gtgcggctct acgcgggaca gggaattgag cggcagttcc
      301 cgtggcgttt tttggggaac tcgctcaact ttcagatgat ttacaccccc gcttttttcc
      361 tggtcgcctg ggcggtggtg ctggggatga gcattttctt cccgcacttt ttcctgtcgg
      421 gccgggtcag gtgagccgtg cgcaccttta tcgccgttga actgcccgaa gaagtgaaaa
      481 cccgggtagc cgaggtttcc gccggcctca gcaagaccgg cgccgacgtg aaatgggttg
      541 aactccagaa cctgcatctg acgttgaagt ttttggggga ggtcggccag gagaagatcg
      601 aagcggtgtt tgaaaaaact caacatgcag cggcaggtac caaagcgttc caactggcct
      661 tggagagcac cggctgtttc cccaatccca aaaggccgcg ggtgatctgg atcggacttt
      721 ctgctggcaa aaccgagttg gtgcagttag ccgcccgcct cgaaaacgaa atggcctcct
      781 tgggatttcc ccgggaagac cggccttttg ccggacattt gaccatcggc cgggtgcgct
      841 cgccgaaaaa tttagataga ttactggcag cgatgaatgg gacggctttc agccatagcg
      901 gggtgacggt ggatcacgtt gtcgttatga aaagcgatct gacgccccgc gggccggttt
      961 acagccgcct gaaaacgatt accttagaac cgtagaggga cttatgccgg tagaaatcgc
     1021 ccaggaaaaa aggcgcgcct tagaccaggc ggtgacccag attgaacggc agttcggcaa
     1081 gggcgcgatc atgcgcatgg gggaagatca taaggtcgca gtgccggtga tttccaccgg
     1141 ctctgtcgct ctggatgccg ccttaggcgt tgggggggtc ccccgcggcc gggtggtgga
     1201 gatcttcggc ccggaatcat ccggcaagac cacgctcgcg ctccatatca ttgccgaagc
     1261 gcagaaagca ggcggcgtgg cggccttcat cgatgccgaa catgcgctgg atacgaccta
     1321 tgccaaacat ctgggcgtcg atctcgacaa cctccttatc tcccagcccg acaccggcga
     1381 gcaggcgtta gagattaccg aaactttggt tcgctccggc gcggtcgatc tggtcgtcat
     1441 cgattcggtg gcagccttag tccccaaagc cgaaatcgaa ggcgagatgg gcgacgccca
     1501 catggggctt caggcgcgct tgatgtccca ggcgctccgc aagctgaccg gcaccatcag
     1561 caagtcgaag agctgcgttt tgttcaccaa ccagatccgg atgaaaatcg ggattatgtt
     1621 cggcaacccg gaaaccacca ccggcgggaa cgcgctcaag ttctacgctt cggtgcgact
     1681 ggatatccgc cggatttcaa cgatcaaaga aggcgagcag gtcgtgggct cgcgcacccg
     1741 ggtgcgggtg gtcaagaata aactggcccc gcccttcaaa gaagcggagt tcgacatcac
     1801 ctacggcatc ggcatttccc gcgaagggga attggtcgat attggctccg acctgaaaat
     1861 tctggaaaaa tcgggcacct ggtactcctt caacggcgag catctcgggc agggccgcga
     1921 caatacgcgg cagtacctca aggactaccc cgatgtggcc aataaaattg ccgccaagat
     1981 tcgcgagcgg ctggggctgg tcaaagtcga atccgcctca ggcggaaagg aacagccgga
     2041 gaagaaagaa ccggctaagg ccaaaaagta gccagagacc ataaaccagt ggccagccga
     2101 agcggagcga aggccccgcc caaggcgggg tagccagcac ggaaagagat gtgggtcagg
     2161 gtgtcattac gaagattgcc cggcaaaaac gtgctcgaga gcgtgtttcg ctttacgttg
     2221 acggccgatt tctttgtggg ctggatgaag ttgcgttcca cgaactacat ctgaaagagg
     2281 gcgatcgtct ttcctcttac agtcagcagg aactcaaagc ccagagcgtg gcggggaagg
     2341 ccaaagacgc cgcgctacgg cttctggcca tccggccccg cagccggaaa gaattagccc
     2401 ggcggttgca aaagaaagga tatggcgccg ctgaaattga gtcggttctt catcaattac
     2461 aagaggaaaa cctgctggac gatgagcgtt tcgcccggct gtacgccgaa caccgcctga
     2521 ccagtcggca tatcggccag cggatgcttc tggcggaatt gctgcagaaa ggaattaacc
     2581 gcacggtcgc gcagtcggtc gtaaatgaga taactggtga tgaccgcgaa aaaatcgagg
     2641 cacgtgtgtt tgcggagaag aaattgcggt tgctttcgaa
//
LOCUS       DBNGZA010000115        10500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_340140, whole genome shotgun sequence.
ACCESSION   DBNGZA010000115 DBNGZA010000000
VERSION     DBNGZA010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_340140"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..1699
                     /locus_tag="AC16FU_02515"
     CDS             536..1699
                     /locus_tag="AC16FU_02515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR2993278.1"
                     /translation="MKAVTARDERKTLDDAKKELDEAKRKFEELKKEREEEREAIEII
                     AAEKSKGFPWLARAYADYFHLQGLQEADHLEHKPHPAQVSADRVREIAGERRTIEEKL
                     RIAQWIIIYYQKLFPFLEDVIGNLDDEILQKILSRNIEKPIKEVAEVGIDPVRIYLSY
                     LPEEEYQKLSLIERNQLALDRYWKRKKSDWEIGRDYERYIGYLYEKDGYSVSYQGIIK
                     GYEDLGRDLICTKDNETEIVQCKYWSQYKTIHEKHINQLLGTTVEYWIQSQNVKNLQI
                     ELLPEFLKHCGITAVFITSTKLSERAKLFGETLGVKIRENFPIQQYPSIKCNVSRKTG
                     EKIYHLPFDQQYNRTLVEEERNECYVETVEKAERLGFRRAWRWRGPEQENETP"
     gene            1686..2981
                     /locus_tag="AC16FU_02520"
     CDS             1686..2981
                     /locus_tag="AC16FU_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR2993279.1"
                     /translation="MKLHNKIFLGLLLGALTGVGSHCLFYPDPRLEWVVANLAYPVGQ
                     IFFRLIFMVVLPLIFSALVLGVSELGELKRLGRVGARTFGYTAIVSTISVLIGVGLVN
                     IVKPGAGLEPETRERLIASISGPGMEKEKAQVMEKAKEAQSVPETIVNLIPRNPLGAA
                     VNAFQGEILAFMVFAVLFGIALALIKSERTKPLVGFLEGVYDVVMKLIEIAMKLAPYG
                     VAALIFTATARFGLEIIKSLSQYVLVVVAGLAIHQFVVYSILVKSLARLSPKLFFSRI
                     GEVMITAFSTSSSNATLPTTMRVSEEKLGIPRKVGGFVLTLGSSLNQNGTALYEGVTV
                     LFIAQLFGVHLSIGAQLTVVLMSILAGIGTAGVPGASLPLIVIVLQSVGVPGEGIVVI
                     LGVDRILDMCRTVVNVTGDIAAAAYVARKENLLKVPERV"
     gene            3421..3750
                     /locus_tag="AC16FU_02525"
     CDS             3421..3750
                     /locus_tag="AC16FU_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993280.1"
                     /translation="MKKFALILLAAAVAFMFSAPAFAQGGQATTQQAEKPAAKKEMTK
                     DTAAAHQAQTQHPAGTAEKSKPAAHPQAASHKGKARKHHTAMKAEPMKAKPDTEKVKQ
                     QSTGTPH"
     gene            4145..5782
                     /locus_tag="AC16FU_02530"
     CDS             4145..5782
                     /locus_tag="AC16FU_02530"
                     /inference="COORDINATES: protein motif:HMM:NF021445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR2993281.1"
                     /translation="MKKRSLFVWTVALVTLASGVVNLVSVTSPALPERRAILREVFPL
                     EFLHLSRFLTLIIGFALVISAFNVYKRKKRAFQGVVLLTVLSVVFHLTKGLDYEEALL
                     ALVFLGLLAAARKRFTVKSSLPDLRSGVIRLGIAALVAFSYSVAGFWLLDKKEFGIDF
                     TLADSIKRTLLFFSLVGDPAIVPQTRHARWFVDSLYVMTATAVGYSLYALFRPALYKF
                     RTLPLERARVAEILSRHGRSALDYFKTWPDKSYFFSPSQKTFLAYRVAGNFALVLSDP
                     VGPEEEIEETVHDFMELCDENDWGLAFHQTLPDLLPIYRRLGFRKLKIGDDAIIDLTG
                     FNLDGRGMKSFRHKVNQLEKAGIQIQIHEPPVSEAALLQVREVSDEWLQLPGRRERGF
                     TQGKFEPEYVRSTPLFAALDGEGRMLAFVNLIPSYRPGEATIDLMRHRLQVPNGLMDY
                     LFVKLFLHQKEQGFQRFNLGMAPMSGFQEKEEAAPEERAVHYFFQRLNFLFSYVGLRQ
                     YKAKFASFWEPRYLVYRSVHSLPRLTLALGKVTELKE"
     gene            5791..6579
                     /locus_tag="AC16FU_02535"
     CDS             5791..6579
                     /locus_tag="AC16FU_02535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR2993282.1"
                     /translation="MKSRFCYGMLGAAVLFPAWLSAQKPEAGKNAFVIRSQRQDIYFY
                     PAKAEAGRWQGTLFFAPGDGGWRGFAVTLADSLSAWRYEVYGLDTRRYLESFTGKTGL
                     READVMRDFRQMARWLAGDRERPVTLLGWSEGAGLCLLAAAAEENKKTFNGLITLGLP
                     DTAFLGWKWSDFISQITKKDPMEPKFSTTEYLPKVTPLPFLLIQSSHDEYVSVATGRR
                     LFSAAREPKRFAFIDARNHRFEGNQKEFFRVLHEGVAWINPTPR"
     gene            6555..7604
                     /locus_tag="AC16FU_02540"
     CDS             6555..7604
                     /locus_tag="AC16FU_02540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2993283.1"
                     /translation="MDQPDAPLTIIPRLPAKSARRFFQPVFGYLLAAAGLVWVFYDIR
                     VEKLWHTLPAIDWWWVVPAVVCDTLSYVCQGLRWQLLLRPVGTLPIGRATQAVYAGLF
                     TNEILPMRIGEFVRAYLVSRWISAEFATVIPSIVVERLLDGVWLALAIGLTAIFIPLP
                     KDLLEAGDFLGIIILAATGFFLYLIYRRRNVPADHWGLSPGWKPVRVLLSMAGRLADG
                     LRKIGTSRFLAGAAGVSLLMFLFQALAFWLVMIAYGFQQSFWVGAVVFLIVHLGTALP
                     NAPSNVGTYQFFTVVGLTLFGVEKTAATGFSVVVFLILTVPLWLIGSFALSRSGTTLS
                     AIRSEIKRLLSAVRK"
     gene            7707..8231
                     /locus_tag="AC16FU_02545"
     CDS             7707..8231
                     /locus_tag="AC16FU_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR2993284.1"
                     /translation="MWIFNHTLLEIALRAAIIYVVVLIGIRLAGKREVGQMTPFDLVL
                     LLLIANAVQNAMTGPDTSVTGGLVAAGTLLVANVVVSRLVWRYKKIRRLAEGTPTLLI
                     HSGKVLTENLAKEKLTTEELEQALREHGVASAGEVSQAVLEIDGAISVLRKDEMPAVA
                     RPHHRIRFVGRKSI"
     gene            8601..9935
                     /locus_tag="AC16FU_02550"
     CDS             8601..9935
                     /locus_tag="AC16FU_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2993285.1"
                     /translation="MPPNHHQNKGKSAPRLFRALRNRNYRLFFGGQSTSLIGTWLTRV
                     ATSWLVYRLTHSAFLLGVVGFAGQIPTFLLAPLAGVLVDRWDRRRLLVLTQVLSMFQS
                     LSLAFLALAGLIAVWQIILLSILQGCINAFDTPARQSFVVEMVEKREDLPNAIALNSS
                     MFNSARLIGPSLAGLLIAAVGEGYCFLLDGLSYLAVIGSLWAMKITTRNREARTTEIW
                     HGLKEGFAYAFGFVPIRNILLLIALVSFTGMPYLVLMPVFAKDILYGGPHTFGFLMTA
                     AGLGALAGAIALASRKSVRGLGRIIALAAGLFGLSLVAFSFSRLLWLSLAVLVVTGFG
                     MIVQMASSNTILQTIVDDDKRGRVMSFYTMAFLGMAPFGSLFAGSLASSIGAPYTVLI
                     GGLACLMGAALFARQLPLLREKIRPIYARMGIIPEVASGVQMATQLTTLPKE"
     gene            complement(10192..>10500)
                     /locus_tag="AC16FU_02555"
     CDS             complement(10192..>10500)
                     /locus_tag="AC16FU_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2993286.1"
                     /translation="VMNADGTGRKQITQNGSANFCPFFSPDGRKIIFASNMADPKGRN
                     FDLYLINFDGTGLERVTYNETFDGFPMFSSDGKKLVFASNRHNAKPGETNIFIADWVW
                     "
BASE COUNT         2207 a         2893 c         2972 g         2428 t
ORIGIN      
        1 tttttccaca cccgttttcg gaggtacttc cgggctttcc gctccgtctg tatccgtcga
       61 gaaaacgcat ccgtggggta catgacacca gtatacttct ggtgtcaaat cctttataat
      121 caccaaagat taagcaaaaa ttgacttctc aaaaacaagt tgatgcttca ggaattttag
      181 cgattaaacg tgaattgaaa ttccactttc ttttgattgt gttgtcaatc ggttgccttg
      241 catttcttct actatatctc tttttacctc agcagaaatt gtcttcaata ttagagaagt
      301 taggagtggc tctcgtcgtc gccctcgttt tgtggctaat aagaattgtc ttgagacgac
      361 tcagaatgta atttacaggt tgcgtcagga gttgctcctg acgctgtgtc gggactaagt
      421 cctgacatga attattaaag ccttaataaa tatggaactc ttgataatta taggcataat
      481 cgtcttaata tgggccattg gaaagatttc tgcctacttg cagcaaagac aaatagtgaa
      541 agccgttacc gcgagagatg aaaggaaaac cttagacgat gcaaaaaaag aattggatga
      601 agcaaagaga aaatttgagg aattaaaaaa ggagagagag gaagaaagag aagcaataga
      661 aatcattgcc gcagaaaaaa gtaaaggttt tccttggctt gcgcgagcat acgccgatta
      721 ctttcacctt caaggtcttc aagaggccga ccatttagag cacaagcctc atcctgctca
      781 ggtttcagcc gatagggttc gtgagatagc gggagagcga agaactattg aagagaaatt
      841 gcgcattgcc caatggatta ttatttacta ccaaaaattg tttccttttc tagaagatgt
      901 tataggaaac ttagatgatg aaatcctcca gaaaattctc tctcgcaaca tagaaaaacc
      961 aattaaggaa gtcgcagaag tcggcataga ccctgtaagg atatacctat cctatttacc
     1021 agaagaagaa tatcagaagc tttccttaat tgaaagaaat caactcgcct tagatcgcta
     1081 ttggaaaagg aagaaatccg attgggaaat cggtagagat tatgagcgat atattggcta
     1141 cttatacgag aaagatggtt attctgtttc ttatcagggc attattaagg gttatgaaga
     1201 tcttggaagg gatttgattt gcactaaaga caatgaaact gaaatcgtac agtgtaagta
     1261 ttggtctcaa tataagacga tccatgagaa acatataaat caattacttg gtactacggt
     1321 ggagtattgg attcagtctc aaaatgttaa gaatctacaa atagagttgc tcccagagtt
     1381 tctgaaacat tgcggtatca cagcagtttt tatcacctct acaaaacttt ctgaaagagc
     1441 caaacttttt ggagaaactt taggtgttaa aataagggaa aatttcccta ttcagcagta
     1501 tccatcaatt aaatgcaatg tttctcgaaa gactggagaa aaaatttacc atttgccttt
     1561 cgatcaacaa tataatagaa cccttgtaga agaagagaga aacgagtgtt atgtagaaac
     1621 tgtagagaaa gccgagagac ttggtttccg tcgtgcgtgg agatggagag ggccagaaca
     1681 agaaaatgaa actccataac aaaatcttcc tcggcctcct tctgggagcc ctgacgggtg
     1741 tcggctccca ctgtctgttt taccccgacc cgcgactgga gtgggtggtg gccaatctgg
     1801 cctatccggt ggggcaaatc tttttccggc tcatcttcat ggtggtcctg ccgctcatct
     1861 tttcggctct ggtcttgggc gtctcggagt tgggtgagct caagcgtttg ggccgggtgg
     1921 gcgcgcggac cttcggctac accgccatcg tcagcaccat ctcggttctc attggcgtgg
     1981 ggctggtcaa tatcgtcaag ccgggcgcgg gattggagcc ggagacaagg gagcggctca
     2041 tagcgagcat ttccggcccc ggaatggaaa aagaaaaagc gcaggtgatg gagaaggcga
     2101 aggaagccca gtcggtgccg gagacgattg tcaacctcat cccccggaac ccgctcggcg
     2161 cggcggtcaa tgcctttcag ggcgagattc tggcctttat ggtctttgcg gtgctgtttg
     2221 gaattgcgct ggcgctcatc aagtcggagc gcaccaaacc cctcgtcggc ttccttgaag
     2281 gtgtctacga cgtggtgatg aagttgatcg agatcgccat gaaattggcg ccctacgggg
     2341 tggccgcgct catcttcacc gccaccgccc gcttcgggct ggaaatcatc aaatcgctct
     2401 cgcagtacgt tttagtcgtc gtcgccgggc tggcgatcca ccagttcgtg gtctattcga
     2461 ttttggtcaa atcgctggcg cggctttcgc ccaaactttt cttctcccgc atcggcgagg
     2521 tgatgatcac cgccttctcc acttcttcct ccaacgccac cctgccgacc accatgcgcg
     2581 tctccgagga gaaattgggc atcccgcgca aagtgggcgg gttcgtcctg accctcggct
     2641 cctcgctcaa ccagaacggc accgcgctct acgaaggggt gacggtgctc ttcatcgctc
     2701 agttattcgg cgtgcatttg agcatcggcg cgcagttgac ggtggtcttg atgtcgatct
     2761 tggccggcat aggcaccgcc ggcgtgccgg gggcgtcgtt gcctttgatt gtgatcgtcc
     2821 tgcagagcgt gggcgtgccc ggggagggga ttgtggtgat tttgggcgtc gaccgcatcc
     2881 ttgatatgtg ccggacggtc gtcaacgtga cgggggacat cgctgcggcg gcgtatgtgg
     2941 cgcggaagga gaatttgctt aaagtgccgg agcgggtgtg aatcgtcttt ccctctcctt
     3001 ttaggggagg gtggtcgcag accgggaggg gtcacgattc ttaagcttga cctcaccctg
     3061 ccctctcctc aaagaggagg gacaaatctc tcaaaaataa tcgtagcgct ggttgcttct
     3121 gtaacttaag cctcgattct ccctcccctt agaggggagg atggctgaaa gccaggaggg
     3181 gtcaaacttt tcaaacttga cctcacccgg ccttcggcca ccctctcctc aaggagaggg
     3241 aaataattct cacacaacgt ccgcaggtac gtatctaaac ttgattgaag taatggcgct
     3301 caaatggcat ctggagactc tcaaggaggg aggtcaatct tgtcgataag gaaaccagtc
     3361 ggaactgccg acttagtatc cgtaactcgt tcactcgcaa ccgaaagagg aggtgagaaa
     3421 gtgaagaaat tcgcgctcat cctcctggcg gccgccgtgg cgtttatgtt ctcggccccg
     3481 gcgtttgccc aggggggcca ggccacgact cagcaggccg aaaagcccgc cgccaagaag
     3541 gaaatgacca aggacaccgc cgcggcgcac caggcgcaaa cgcagcaccc ggctggcaca
     3601 gcggaaaaga gcaaacctgc ggcgcacccg caggccgcaa gccacaaggg caaagcccgg
     3661 aagcatcaca cggccatgaa ggcggaaccg atgaaggcca agccggacac tgaaaaggtg
     3721 aagcaacagt caactggcac gcctcattag atgaggcaag ccgaggcgca gaagcagtaa
     3781 caatgtggga ggccgtccat tctgaacggg acggcctccg gcgctatcag accggcggcg
     3841 tacgcggcaa tgaaaaggct tggctctccg tgccggagcg ggtgtaaccc gacccctccc
     3901 gtccgtacgg acaccctccc ctcaaagggg agggaaaatg agagtctaat tcaactgtag
     3961 cgccgctctc ctgagcggcg agaaaagttt tttcctccac tacgaagaaa gcgaaaattt
     4021 ctctcgaccc ctcgatgcag ggccgctccc cgagctaagc ccgacggcat ccatctcctt
     4081 ttagcggcgc cctttataaa atcgtagcac tgtagtagtt taaaagggcg accggtcgtc
     4141 ctctatgaaa aaacgctctc ttttcgtctg gacggtggcg ctggtcaccc tggccagcgg
     4201 ggtggtgaac ttggtctcgg tcaccagtcc agccttgccg gagcgccggg cgatcctgcg
     4261 cgaggtcttt ccgctcgaat tcctccacct ctcccgcttc ctcacgctca tcatcggttt
     4321 tgctttggtc atttccgctt tcaacgtcta caaacggaag aagcgtgcgt tccagggcgt
     4381 ggtgctcctg acggtcctgt cagtagtttt ccacctgacc aaagggctgg actacgaaga
     4441 ggcgcttctg gcgctcgttt ttctcggtct gcttgcggcc gcgcgcaaac gattcacggt
     4501 caaaagcagc cttcccgacc tgcgctcggg agtgatccgt ctggggatcg ccgctctcgt
     4561 ggctttttct tacagcgtgg ccgggttctg gctcctggac aaaaaggagt tcggcatcga
     4621 cttcacctta gccgattcga tcaagcgcac gctgcttttc ttttccttag taggcgatcc
     4681 ggcgattgtc ccgcagacgc gccacgcgcg ctggttcgtc gactccctct acgtcatgac
     4741 cgccaccgcg gtcggctact cgctctacgc cctgttccgg cccgccctgt ataaattccg
     4801 gaccctgccc ctcgaacgcg cccgggtggc ggaaatcctc tcccggcacg gccgctccgc
     4861 gctcgactat ttcaagacct ggccggacaa atcgtacttt ttctccccgt cgcaaaagac
     4921 ctttctggcc taccgggtgg cgggcaactt tgctctggtg ctgagcgatc cggtgggccc
     4981 ggaagaagag atcgaggaga ctgttcacga ttttatggaa ctgtgcgacg agaacgactg
     5041 gggccttgcc tttcatcaaa ccctgcccga tcttctgccc atctaccggc ggctgggctt
     5101 ccgaaaactc aaaatcggcg acgatgccat tatcgacctg accggcttca acctggatgg
     5161 ccgggggatg aagagcttcc ggcacaaggt caatcaactg gaaaaagctg gcatccagat
     5221 ccagatccac gagccgcccg tgtccgaggc ggcgcttttg caggtgcggg aggtctccga
     5281 cgaatggctg caactcccgg gccgccggga gcgcgggttc acccagggga agttcgagcc
     5341 ggagtacgtc cgctccaccc cgctttttgc cgctttagac ggggaaggcc ggatgctggc
     5401 ttttgtgaac ctcatcccgt cctaccgtcc gggcgaggcg accatcgact taatgcggca
     5461 tcggctgcag gtgccgaacg ggctcatgga ttaccttttc gtcaaacttt tcctccatca
     5521 aaaagagcag ggctttcagc gcttcaacct gggcatggcg cccatgtccg ggtttcagga
     5581 gaaagaggag gcggcgccgg aagagcgggc cgtgcactac tttttccagc gcttgaattt
     5641 tcttttcagt tacgtcgggc tgcgccagta caaggcaaaa ttcgccagtt tctgggaacc
     5701 gcgctacctg gtttaccgca gcgtgcacag tctgccgcgg ctgaccctgg cgctggggaa
     5761 agtgaccgag ctcaaggaat agggggaagt gtgaaaagtc gcttttgcta tggcatgctt
     5821 ggggcggcgg ttcttttccc ggcttggctc tctgcccaga aaccagaggc cgggaaaaac
     5881 gctttcgtca ttcgcagcca gcgccaggat atttacttct accccgcaaa ggccgaagcg
     5941 ggccgttggc agggcacgct ctttttcgct cccggcgacg gcggctggcg cggatttgcc
     6001 gtcacgctcg ccgattcgct ttccgcctgg aggtatgagg tctacgggct ggataccagg
     6061 cgctacctgg aaagtttcac cggcaaaacg ggcttgcggg aagccgatgt catgagagat
     6121 ttccggcaaa tggcccgatg gctcgccggg gatagggaga gaccggtcac cttactggga
     6181 tggtcggaag gcgccgggtt gtgccttctg gccgcggctg cggaagagaa caagaaaacc
     6241 tttaacggct taattaccct gggcctgccg gacaccgcct ttttgggctg gaagtggtcc
     6301 gacttcattt ctcaaataac caaaaaggat cccatggagc cgaagttctc gacgaccgag
     6361 tatctgccca aagtcactcc gctccccttt cttttgatcc agtccagcca cgacgaatat
     6421 gtttcggtgg caacgggcag gcggctcttt tccgccgccc gggaacccaa gcggtttgcg
     6481 ttcattgacg cgcggaacca ccgctttgag gggaaccaaa aagaattctt ccgcgtgctg
     6541 cacgaggggg tggcgtggat caacccgacg ccccgctgac cattattccc cgtctgccgg
     6601 caaaatccgc ccggcggttt ttccaaccgg tcttcggcta ccttttggcg gctgcaggtc
     6661 tggtatgggt tttctacgac atccgggtgg aaaaactctg gcacaccctc cccgccattg
     6721 actggtggtg ggtggtcccc gcggtcgtct gcgacaccct gagttacgtc tgccagggtc
     6781 tgcgctggca actccttttg cggccggtgg ggacgctgcc gatcgggcgg gcaacccagg
     6841 ccgtgtatgc cggcctcttc accaatgaaa tcctgccgat gcgcatcggc gaattcgtcc
     6901 gggcctactt agtctcgcgc tggatttccg cagaatttgc gaccgtcatt ccctccatcg
     6961 tggtggagcg tctcctggac ggagtttggc tggcgttggc catcggccta accgccatct
     7021 tcatcccgct ccccaaagat ttactcgagg cgggcgattt tttggggatc atcattctgg
     7081 ccgccaccgg tttctttctc tacctgatct accgcaggcg caacgttccg gctgaccatt
     7141 ggggactgtc cccgggctgg aaaccggtgc gcgtgcttct gtcgatggca ggacgcctgg
     7201 ctgacgggct ccgaaagatc ggaacgtccc gcttcctcgc cggggccgct ggcgtatcac
     7261 tcctcatgtt ccttttccag gccctggcct tctggctggt gatgattgcg tacggtttcc
     7321 agcaatcctt ctgggtgggg gcggttgtct ttttgatcgt ccacctgggg accgccctgc
     7381 ccaatgcgcc ctccaacgtg gggacgtacc aatttttcac cgtcgtcggt ttgacactct
     7441 tcggagtgga aaaaacggcg gccaccggct tttccgtggt ggtgttcctg attctgacgg
     7501 ttcccctgtg gctcatcggc tcgttcgccc tcagccgcag cgggacgact ttgtccgcga
     7561 tccgcagtga aatcaagagg ctattgagcg cggtcaggaa gtgaagccgg cgttttcatt
     7621 ttcggttttt ctctcgatca cgaacttttc tttcggcgcc ggctttccgc ttgacctcgt
     7681 ctccaaccga gcgtagaatc tcctccgtgt ggatcttcaa tcacaccctg cttgaaattg
     7741 ccctgcgagc ggcgattatt tacgtcgtcg tcttaatcgg catccgcctc gccggcaagc
     7801 gcgaagtcgg ccagatgacg cccttcgatt tggtcttact gcttctgatt gccaacgcgg
     7861 tgcagaatgc gatgaccggg ccggatacgt cggtcactgg cggcttggtg gcagcgggga
     7921 cgcttctggt ggccaatgtc gtggtgtcgc gcttggtctg gcgctataaa aaaatccgcc
     7981 ggttggcgga ggggacgccg actctgctca ttcactcagg aaaagttctc acagaaaacc
     8041 tggcgaaaga aaagttgacc actgaagaat tggagcaggc cctgcgggag catggcgtcg
     8101 ccagcgccgg cgaggtgagc caggcggtgc tcgaaatcga cggcgccatc agcgttctgc
     8161 gcaaggacga aatgcccgcc gttgctcgtc cgcaccaccg gataagattt gtgggcagga
     8221 aatcaatcta atcctctttc tgacctcacc cggcctacgg ccaccatctc ctacaggaga
     8281 gggagaatct tccataacaa acgtagtgca ggttggtctg cgtaacctgc accaaaccat
     8341 ctttggcgcg gattaccaaa acccttcgat ccgcctcagg cggactcagg ggcaagccaa
     8401 tctgcgccac cgtgtgttta gagagatgtt ccccttgtag gggagggtgt cccggtgtat
     8461 cgggacggaa ggggtcaggc ggggagatac tgcgctggcc gatgcaaacg gaaaaggaat
     8521 tggccagatg gcggcaacgg caaacaaaca gtaaatgtgg gtgcccgtaa aagttagcaa
     8581 gcagtcccgc agataaatag atgccgccga atcatcatca gaataaggga aaaagcgcgc
     8641 cgcggctttt tcgcgcgctg cggaacagga attaccgcct ctttttcggc ggccagagta
     8701 cctcactcat cggaacttgg ttgacccgcg tcgccactag ctggctggtc tatcgcctga
     8761 cgcattcggc cttcttactc ggcgtcgtcg gcttcgccgg tcaaatcccg acttttctcc
     8821 tcgcccctct ggccggtgtt ttggtcgatc ggtgggaccg gcggcggctt ttggtgctga
     8881 cgcaggtttt gtcgatgttc cagtccctct cgctggcatt tctggccctg gctggtctca
     8941 ttgccgtctg gcagattatt ctcctctcca tcttgcaggg gtgcatcaat gcgttcgaca
     9001 cgccggcccg gcagtcgttt gtggtggaaa tggtcgaaaa acgggaggac ctgcccaacg
     9061 ccattgccct gaattcctcc atgttcaaca gcgcgcgctt gatagggcca tccttagccg
     9121 ggctgctcat cgccgcagtc ggggaagggt actgttttct tttggacggc ctcagctatc
     9181 tggcggtgat cgggtcctta tgggccatga agataactac gcggaataga gaagcccgca
     9241 ctacagagat ctggcacggc ttgaaagagg gcttcgctta cgccttcggt tttgtgccga
     9301 tccggaacat cctgttgctc atcgcgctgg tcagtttcac gggtatgccg tacctggtgc
     9361 tcatgccggt tttcgccaaa gacatcctgt acggcggtcc gcacaccttc ggcttcctga
     9421 tgaccgccgc aggactgggg gcgctggcgg gcgccattgc tcttgcctcg cgcaaaagcg
     9481 tccgcggact ggggaggatc atcgcgctgg cagccggcct tttcggcctg agtctggtgg
     9541 ctttttcttt ttcccgcctg ttgtggctct ctctggccgt tttggtcgtg accggtttcg
     9601 gtatgatagt acagatggcc tccagcaata cgatcttgca aaccattgtg gacgacgaca
     9661 agcgcgggcg cgtgatgagc ttctacacga tggcctttct gggtatggca cctttcggca
     9721 gcttgttcgc gggaagtctg gcgagcagca tcggcgcgcc gtatacggtg ctcattggcg
     9781 gccttgcctg cctgatgggt gccgccctgt tcgcccgcca actccccctc ctgcgggaga
     9841 agatacgccc catctacgcc cgcatgggca ttatacctga agtggcttcc ggagtgcaga
     9901 tggccacgca actgaccacg ttgccgaaag agtaacaaga atcattttcg cctctctggc
     9961 cttcaagggg ctcaagtaaa agagagcggt gctacaggga ggtagatttt ccctctcctt
    10021 gaggagaggg tggccggagg tcgggtgtgg tcagtcccgc tcagggacga gcgggcctac
    10081 cagttggcga ccgggggcgg ccatacggcc cgtaggggtc gccctacgcg ggagaggcat
    10141 atatcccatg taggcgcggc gcctcgccgc gcaggcgcat aaagtcgagt tctaccacac
    10201 ccaatccgcg atgaagatgt tcgtctcccc cggcttggcg ttatgccggt tggaggcgaa
    10261 gactaacttc ttgccgtcag acgaaaacat cgggaagccg tcaaacgtct cgttgtaggt
    10321 gacccgctcc agacccgtgc cgtcgaagtt tatcaaatat aagtcgaaat tacggccctt
    10381 cggatcggcc atattcgacg cgaaaatgat tttcctgccg tccggcgaaa agaaggggca
    10441 gaagttcgcc gagccgttct gggtgatttg tttccggccc gtgccgtcgg cgttcattac
//
LOCUS       DBNGZA010000116         1758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2356239, whole genome shotgun sequence.
ACCESSION   DBNGZA010000116 DBNGZA010000000
VERSION     DBNGZA010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2356239"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16FU_02560"
     CDS             complement(<1..224)
                     /locus_tag="AC16FU_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993287.1"
                     /translation="MAEVAVREPKVKPKPVTEKKTEKNWGKVVQVIGPTVDCEFPSDR
                     LPNMLSAIKIEDEKRGIKLTVEVALHIGDNV"
     gene            complement(330..1190)
                     /gene="atpG"
                     /locus_tag="AC16FU_02565"
     CDS             complement(330..1190)
                     /gene="atpG"
                     /locus_tag="AC16FU_02565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR2993288.1"
                     /translation="MPSIRDLRRRIRSVENTKQITRAMQMVAAARLRRAQAKVEAARP
                     YASKMQEMLASLASAATALEHPLFQKREVRKVGLVVITSDRGLCGSYNANVIRNAELF
                     LRERAPDNTLLALVGKKGFDFYHRRRWELRLKYLDLGGNLVLTRTRRIASDLTNLFLS
                     GEVDEIHLLYTRFVSTVTYRVTREKFLPIEPQKSAGDSAGALDYIFEPGPEQIFQTLL
                     PRFCLTRVQIALAEAFASEHGSRMIAMTSATQNALEMIDHLTLVRNKVRQASITKEML
                     EIVGGAEALK"
     gene            complement(1312..>1758)
                     /locus_tag="AC16FU_02570"
     CDS             complement(1312..>1758)
                     /locus_tag="AC16FU_02570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289591.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR2993289.1"
                     /translation="NVGISVSRVGGKAQVKAMRKVAGRLRLDLAQYRELAAFAQFGSD
                     LDETTKAQLIRGERTVEILKQDQYVPMPVEKQVMIIWVATHGHLDDLLIDAVKRFETE
                     FYAFMEKEYPEVPHTIAQTKELDEKIEKRLEEATQKFKEKFKSNLK"
BASE COUNT          331 a          541 c          448 g          438 t
ORIGIN      
        1 acgttgtcgc cgatgtgcag cgccacctcg accgtcagct taatgccgcg cttttcgtct
       61 tcgattttga tggcgctaag catattcggc agccggtccg agggaaactc gcagtccacc
      121 gtcggcccga tcacctgtac gaccttgccc caattctttt cggtcttctt ctccgttacc
      181 ggcttgggtt tgactttcgg ttctcgcacc gctacttcag ccatgcactc tccttaatgg
      241 taataagttg tttctgtatt tttcccaaat ttattttgtt ccggccagtg atctttccca
      301 gtgtccggtc tcctgtgttc tgcatgctgt catttcagcg cttccgctcc cccgacgatt
      361 tccaacatct ctttggtaat cgacgcttgc cgtaccttgt tccgcaccaa agtcaggtgg
      421 tcgatcatct ctagggcgtt ctgggtggcc gaagtcatgg cgatcatccg cgaaccgtgc
      481 tcggaggcaa aggcctccgc caaggcgatc tgcacccgcg tcagacagaa gcgcggcaga
      541 agcgtctgga aaatctgctc cggacccggt tcaaagatat aatccagcgc acccgcggag
      601 tcacccgccg acttctgtgg ctcgattggc aaaaacttct ccctcgtgac gcgataagta
      661 accgtcgaga cgaagcgggt gtagagcagg tgaatctcat ccacctcacc cgacaaaaag
      721 aggttggtga ggtcggaggc aatccgccgc gtccgggtta atacgaggtt gccgcccaga
      781 tcgaggtatt tgaggcgcag ctcccacctg cgccggtggt agaaatcaaa tccttttttg
      841 ccaacgagcg cgaggagcgt gttgtccggc gccctctcgc gcaggaacaa ttccgcattg
      901 cggatgacgt tggcgttgta cgagccgcac agcccccggt cggaagtaat gaccaccagc
      961 cccacctttc tcacctcgcg cttttgaaag agcgggtgct ccaaggccgt ggccgcggaa
     1021 gccagagatg ccagcatctc ctgcatctta gaagcgtaag gccgggctgc ttccactttc
     1081 gcctgcgccc gcctgaggcg ggccgccgcc accatttgca tggcccgggt gatctgcttg
     1141 gtgttttcga ccgaacgaat ccggcgtctc aagtcacgta ttgacggcat gacgtatcat
     1201 aaatatgact cttttgcgca tctctttttt gtagcgcggg atggtcttcg tatcccgcgc
     1261 tggcgcaggt tccagaaacc aacctgcgct acaagtctct agaaactgtt actacttcaa
     1321 attactcttg aacttctcct tgaacttctg cgttgcctct tccaaccgtt tttcaatctt
     1381 ctcatccaat tctttcgtct gcgcgatggt gtgcggcact tccgggtact ctttttccat
     1441 aaaagcatag aactcggtct cgaaccgttt caccgcgtcg atcagcaggt cgtccaaatg
     1501 gccgtgggtc gccacccaga tgatcatcac ctgcttctcc accggcatcg ggacgtattg
     1561 atcctgcttt aagatttcca ccgtccgctc gccgcggatc aactgcgcct tggtcgtttc
     1621 gtctaaatcg gagccgaact gggcgaaggc tgccagttcc cggtactggg ccaaatcgag
     1681 ccgcaaccgg cccgccactt tccgcatcgc cttgacctgg gctttgccgc cgacgcggga
     1741 aactgatatg ccgacgtt
//
LOCUS       DBNGZA010000117         4129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1613499, whole genome shotgun sequence.
ACCESSION   DBNGZA010000117 DBNGZA010000000
VERSION     DBNGZA010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1613499"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1488
                     /locus_tag="AC16FU_02575"
     CDS             103..1488
                     /locus_tag="AC16FU_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR2993290.1"
                     /translation="MFTRYHIKTKVAPPRSPQPSKFVVKVDRLKFVRMIAGELKESSS
                     LSEVKATIKSRPCLYGVYSRPVGGFAPKSSSCVACYRCVIENPGMVRILPNPDLIEKA
                     QSSGLTPKQISTLWYEAATGRVPVKGAGYNGPFSGPGFDAIWTDMSEIVRPTRDGIHG
                     REYISTGVEIGSRPAFVEFGNPGASRSDNLVRLPIPMLFDAAAIPLPYFQRAAAAAAR
                     QLATLVFVPAAAAGLPGDGRNIIPLVSISDLEKKSDFFKGFKLLEILCNSVDEYRKVK
                     QATGKMIIARLAITLGIEKLITELLREKVEAIHFSPELPGANGTQPALLRDTLRSVHR
                     ILVQERKRDQVTVIAGGAITAAEHVPKAIICGADAVALDLPLVVALQGEFRSGENGRP
                     AGVHLPEFDDDWARQRLVNLVGSWHAQLLEILGAMGLREVRRLRGEMGRAMFAEELEK
                     EFCELFCRSDG"
     gene            1481..2782
                     /locus_tag="AC16FU_02580"
     CDS             1481..2782
                     /locus_tag="AC16FU_02580"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR2993291.1"
                     /translation="MDSVKLKILERHEHKIHRGLTELASGAQGIPESLGNVRWTPELL
                     MTIWEQSRTGRPPTSGLEYKVGRSGGGFDTLNFKFLPQSQWLSETEPIDLSLSLNRRD
                     DRAKITAPLPAYGAGMSFGSVSLQVMLSRARAAKELGTFISTGEGGYPEPLAEYADHV
                     ITQVATGMFGVREETIQRARIVEFKYAQGAKPGLGGHLLGDKVTSEVAAMRESLPWVS
                     LFSPFPFHSVYSVEDHKKHVDWIKAVNPKALVAVKVSTPVDVDMVAVGVYYAGAHILN
                     LDGGYGGTGAAPEIAKKNIAMPIEYAIPKVHKFLEKEGIRDEIVVMASGGIRTAYDVA
                     KAIALGADGCIIGTAEIVAIGCTYCGNCERGRGCPVGITTTDPELSLLINPDWGAQRI
                     VNLYRAWEIQWREILRKLALKSIRELRGRYDLLQHQHNHDA"
     gene            2772..>4129
                     /locus_tag="AC16FU_02585"
     CDS             2772..>4129
                     /locus_tag="AC16FU_02585"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR2993292.1"
                     /translation="MTHNSLTAAESIITSRSALVLPEHLPAHKSEAEGGCGVVGLAAS
                     VPVKGKHLLVPMLQMTNRGNGKGGGIAAVGLSPEQMGVSQEILDNAYLILVAYLNSSS
                     RQAVEERYIKPALDLHQAQKIPTLDDYREVKGLEVPPPEVWRYFGRVKPNVLREFTRK
                     NGLEKHAPQKVEDEFIYQNSFNLNREFYATGERRAFVLSHGRNLYVLKIVGYAQDVVR
                     YYRLEDLQAHIWIGHQRYPTKGKVWHPGGSHPFVGLDEALVHNGDFANYYSITEYLAQ
                     RNIAPLFLTDTEVSVLLFDLLNRVYDYPLEYLIEALAPTTERDFEMLPEEKKRIYQKI
                     QAAHIHGSPDGPWFFIIARNDRKSGAYQLLGITDTSMLRPQVFALQEGEAQIGIIGSE
                     KQAIDATLRSLQSEDSRFWSRADRYWNARGGSYTDGGAFIFSVNGNGNGSAGLTVNGP
                     RS"
BASE COUNT          904 a         1157 c         1254 g          814 t
ORIGIN      
        1 gttgtacttc aggttacgtg aaatcttttg agaagatgaa agcgaagtct atccgaatag
       61 caggacggat cgctacctgt aaaagttaga gcagaaacaa gcatgtttac ccgctaccat
      121 atcaagacaa aagtagctcc accccggtca ccacagccgt caaagttcgt ggtgaaggtt
      181 gaccggctga agttcgtccg gatgatcgcg ggtgaactga aggaatcgtc ttcgctttcg
      241 gaagtgaaag cgacgatcaa aagccggccc tgcctgtacg gcgtctacag ccggcccgtc
      301 ggcggctttg cccccaaaag cagttcctgc gtggcgtgct atcgctgcgt catcgaaaat
      361 ccggggatgg tgcgcatcct gccgaatccg gatttaatcg agaaagctca aagttccggt
      421 ctgaccccca aacagatttc caccctctgg tacgaagcgg cgacgggccg cgtgccggtg
      481 aagggagcgg gctacaacgg gcctttctcc ggccccggct tcgatgccat ctggaccgac
      541 atgtcggaaa tcgtgcgccc gacgcgggac gggattcacg ggcgagagta catctccacg
      601 ggagtggaga ttggctctcg accagcgttc gtcgaattcg gcaacccggg agcatctcgc
      661 tccgacaatc tcgtgcgcct gccgatccca atgcttttcg acgccgcggc catcccgctg
      721 ccgtatttcc agcgggcggc cgccgccgcg gcgcgtcagt tggcgactct ggttttcgtc
      781 ccggcggcgg cagcgggtct tccgggcgac ggaaggaaca tcattccttt ggtttcaatc
      841 tccgatttag aaaaaaagag tgatttcttc aaggggttca agcttctgga aatactctgc
      901 aatagtgtgg acgagtaccg gaaggtcaaa caggccacgg ggaaaatgat tatcgcccgc
      961 ttggccataa cgctgggcat cgaaaagtta ataactgaac ttctgcggga gaaagtcgag
     1021 gccattcact tctctcccga actgcccgga gcgaacggca cccagccggc gctgttgcgc
     1081 gacacgctgc gttcggtcca ccggattttg gtccaagaac gcaaaagaga tcaggtgacc
     1141 gtgatcgccg gaggggcgat taccgccgcc gagcacgtac cgaaggcgat catctgcggc
     1201 gcagatgcgg tcgctctcga tttgcctttg gtggtggcgc tccagggtga atttcgatcg
     1261 ggagaaaacg ggagaccggc gggggtgcat ttgccggaat tcgacgacga ctgggccagg
     1321 cagaggttgg taaacttggt cggctcctgg cacgcgcagc ttctggaaat tctgggtgcc
     1381 atgggactga gggaggtcag acgcctgcgc ggggaaatgg gacgggcgat gttcgcggag
     1441 gaattggaaa aggaattctg cgagcttttc tgtcgaagcg atggatagcg tcaaactcaa
     1501 aattctcgaa cggcatgagc acaaaattca ccgcgggctg acggagctcg cctccggcgc
     1561 gcagggcatc cccgaatcgc tgggaaacgt gcgctggact cccgaactct tgatgaccat
     1621 ctgggagcag tcccgcaccg gaaggccgcc cacgtcaggg ctcgaataca aagtcggccg
     1681 ctccggcggg ggatttgata cactgaattt caaatttctt ccacagtcgc agtggttgag
     1741 cgaaaccgag ccaattgatc tctcgctttc tctgaaccgc cgtgacgatc gagccaaaat
     1801 caccgctccc ttgcccgcct acggcgccgg gatgtcgttc ggctcggtca gtctgcaggt
     1861 aatgctctcc cgggcgcgcg cggcgaagga actgggaact ttcatctcca caggggaagg
     1921 tgggtatcca gagccactgg ctgaatacgc cgaccacgtt atcacgcagg tggccacagg
     1981 catgttcggg gtgcgggaag agacgatcca gcgggcgcga atcgtcgaat tcaaatacgc
     2041 gcaaggagcg aagcccggtt tgggcgggca cctgttgggc gacaaggtca cttccgaagt
     2101 ggcggcgatg cgggagtcgc tcccctgggt ctcgctcttt tcgccctttc cattccacag
     2161 cgtctactcg gtggaagacc acaagaagca cgtggactgg atcaaagcgg ttaatccgaa
     2221 agcgctcgtg gcggtgaaag tttccacgcc ggtggacgtc gatatggtgg cggtgggggt
     2281 ctactacgcc ggtgcgcata ttctgaactt ggacggcggc tacggcggca ccggcgcggc
     2341 gccggaaatc gccaagaaaa acatcgccat gccgattgag tatgccattc cgaaagtcca
     2401 caagtttctg gaaaaagaag gcatccgcga cgagattgta gtcatggctt ccggcggcat
     2461 tcgcaccgcg tacgacgtgg ccaaagcgat tgctttgggc gcggacggct gcatcatcgg
     2521 cacggcggaa atcgtggcca tcggctgcac ctactgcggc aactgcgagc gggggcgggg
     2581 gtgccccgtg ggcatcacca ccaccgatcc ggaactgtca cttttgatca atcccgactg
     2641 gggagcgcag cggatcgtca atctctaccg cgcgtgggaa atccagtggc gggagattct
     2701 gcgcaaattg gctttgaaat cgatccgcga actgcggggc cggtacgact tactgcaaca
     2761 ccagcataac catgacgcat aattccctga ccgccgctga atcgataatc acttcccgca
     2821 gtgccctcgt tttaccggaa catcttcccg cgcacaaaag cgaagccgaa ggcggatgcg
     2881 gagtagtcgg gctagccgcc tccgttcccg tgaaaggcaa gcacctattg gttccgatgc
     2941 tccagatgac caatcgcgga aacggcaaag ggggcgggat cgctgccgta ggactttcgc
     3001 ccgagcaaat gggcgtcagc caggaaattt tggacaatgc ttacttgatc ttggtcgcgt
     3061 acctcaattc ttcctcccgg caggcggttg aagagcgata catcaaaccg gcactcgacc
     3121 tccaccaggc gcagaagatt ccgacactgg acgactaccg cgaagtaaaa ggattggaag
     3181 tgcctccgcc ggaggtgtgg cgctactttg gcagagtcaa accgaacgtt ctgcgcgaat
     3241 tcacccgtaa gaacggtctg gagaaacacg cacctcaaaa ggttgaagac gagttcattt
     3301 accagaacag tttcaatctc aatcgggaat tttacgccac aggagaaagg cgggcgtttg
     3361 ttctctcgca cgggcggaac ctctacgtgc tgaaaatcgt gggctacgcg caggacgtgg
     3421 tgcgctacta caggttggag gatctgcagg cccacatctg gatcggccac cagcgctatc
     3481 ccaccaaggg aaaggtctgg catccgggcg gttcgcaccc gttcgtcggt ttggacgaag
     3541 cgttagtaca caacggtgat ttcgccaact actacagtat caccgagtat ctggctcagc
     3601 gcaatatcgc cccgctcttt ttgaccgaca ccgaagtctc cgtgcttttg ttcgacctgt
     3661 tgaatcgggt ctacgattac ccgctggaat atttgatcga agcgctggcg cctacgaccg
     3721 agcgggattt cgaaatgctg ccggaggaga agaaacggat ttaccagaaa attcaggcgg
     3781 cgcacattca cggctcgccg gacggcccct ggtttttcat cattgcgcgg aatgaccgca
     3841 agagcggggc atatcaactc ctgggtatta ccgacacctc catgctcagg ccgcaggtct
     3901 tcgccttgca ggagggggag gcgcagatag gcatcatcgg gtcggagaag caggcgattg
     3961 acgccacgct ccgcagtttg cagtccgaag acagccgctt ctggtcgcgg gccgaccgct
     4021 actggaacgc ccggggcggc agttacaccg atggcggcgc gttcatcttt tcggtaaacg
     4081 gtaatgggaa cggttcggcg gggctcaccg tgaacggtcc caggagttt
//
LOCUS       DBNGZA010000118         1596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_128770, whole genome shotgun sequence.
ACCESSION   DBNGZA010000118 DBNGZA010000000
VERSION     DBNGZA010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_128770"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC16FU_02590"
     CDS             <1..779
                     /locus_tag="AC16FU_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993293.1"
                     /translation="IASFKKAAEVDPARSDLPIALGGTYARLRKFTEAASYYQRAILL
                     DPGVSGQYVILMYVYVRQDGNTKKARRTLEEATGKVDAAEFNRGWVWLDISDGNYPQA
                     LNRLNGPEAYDNDSVDYYLDKAKIYGLIAQPKPARACYDSARGHLEKQLRTEFENEAL
                     YSQLGIAYAGLGRKEEAIREGKKAVELLPVSRDAWSGPTRILSLARIYVMIGEYDAAV
                     KELEYLLSIPSELTASLLRLDPVWAPLKNHPRIQKLLAGK"
     gene            804..>1596
                     /locus_tag="AC16FU_02595"
     CDS             804..>1596
                     /locus_tag="AC16FU_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993294.1"
                     /translation="MLKAVDLTKQFGKLTAVDHLNLEVKKGEFFGFLGPNGAGKTTTI
                     KMMVGLMRPTSGRVEIGDFDLAAHPLEAKRLLGFVPDQPYLYDKLTVSEFFDFVASVF
                     QLDRRTAAWKKKSLIELFDLGEKEDELIGTLSHGTKQKVALGGALLHEPKVLLLDEPT
                     VGLDPRSARILKDILNELARTGCVVFMSTHIMEIAEAMCDRVGIIDHGKLVAVGNLDD
                     LRRKTKEVGATLEDLFLSLTGGSEYEEVKRFLSAPAPTYAPAAPQS"
BASE COUNT          385 a          421 c          438 g          352 t
ORIGIN      
        1 caatagccag ctttaaaaaa gccgccgagg tggaccccgc ccgttccgac ctccccattg
       61 ctctcggcgg gacctacgct cgattgcgca aatttaccga ggcggcatcc tattatcagc
      121 gcgcaatttt gctcgatccg ggtgtaagcg gccagtatgt aatactgatg tacgtgtacg
      181 ttagacaaga tggtaacacc aaaaaagctc gaaggactct tgaagaggca acgggaaaag
      241 tggatgccgc ggaatttaat agaggatggg tttggctgga tattagtgat ggcaattatc
      301 cgcaggcctt gaaccggctt aacggaccgg aagcgtatga caacgattcg gtggactact
      361 atttggacaa agccaaaata tacggcttaa tcgcccaacc gaagccagcg cgcgcctgct
      421 acgattccgc ccggggtcat ctggaaaaac aattgcgaac agagtttgag aatgaagctc
      481 tttacagtca attgggaatt gcttatgccg gactgggacg caaagaggag gcgatccgcg
      541 aaggtaaaaa agcggtggag ctattgccgg taagccggga tgcttggagt ggtcctaccc
      601 ggattctatc tctcgcccgt atttacgtga tgataggaga atatgatgcc gccgtaaagg
      661 aacttgaata tctgctgtcc ataccgagtg aactaaccgc ctccttgctt cggcttgacc
      721 ccgtctgggc gcctttgaaa aatcacccta gaattcagaa acttttggca gggaaatgaa
      781 gagtaaatcc acttctccca cctatgctca aagccgttga cctgaccaag cagttcggga
      841 aactcaccgc cgtcgatcat ctgaacctgg aggtgaaaaa aggcgagttc ttcggctttc
      901 tggggccgaa cggcgccggg aaaaccacga ccatcaaaat gatggtggga ttgatgcgac
      961 cgacttctgg ccgcgtcgaa ataggtgact ttgatctggc agctcatcct ctggaggcca
     1021 aacgcctcct ggggttcgtg cccgatcaac cctatctgta cgacaaacta accgtgagcg
     1081 agtttttcga ttttgtcgct tcggtcttcc aactcgaccg tcggaccgct gcctggaaga
     1141 agaagtcgct cattgaactc ttcgacctgg gggagaaaga agacgagttg atcggcaccc
     1201 tctctcacgg taccaagcag aaagtggcct tgggtggcgc gctcctgcac gagccgaagg
     1261 tgctcctttt ggatgagccg acggtggggc tcgacccccg ctcggcccgc atcttaaagg
     1321 acattttgaa cgaactggcc cgtaccggct gtgtcgtctt catgtccacc cacatcatgg
     1381 aaattgccga ggcgatgtgc gaccgggtgg gcatcattga ccacgggaag ttagtggcgg
     1441 tcggcaactt agacgacctg cgccgcaaga ccaaagaagt aggtgccacg ttagaagatt
     1501 tgttcctctc ccttacgggc ggctctgagt atgaggaagt gaagcgcttc ctttccgccc
     1561 ccgccccgac ttatgctccg gctgctcctc aaagtt
//
LOCUS       DBNGZA010000119         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_128798, whole genome shotgun sequence.
ACCESSION   DBNGZA010000119 DBNGZA010000000
VERSION     DBNGZA010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_128798"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="argS"
                     /locus_tag="AC16FU_02600"
     CDS             complement(<1..732)
                     /gene="argS"
                     /locus_tag="AC16FU_02600"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR2993295.1"
                     /translation="MNRFVHEIITTLEPRLGLAPEAIAELIEVPPDSQFGDYALPCFR
                     LSKQLRQAPEKIANDLAALFKAANAIQEVTVVGGYLNFRVNQKTFTDETLKEIFSAGE
                     KFGSSDVGKGKTIVIDFSSPNIAKPFGVGHLRSTVIGNALGLLYRKLGHPTLGINHLG
                     DWGTQFGYLIAAFKRWGSEEALASDPIRHLYELYVRFNTEADANPQTAEEGRRWFKKL
                     ESGDGEAVTLWQRFSRTSLESFQKIY"
     gene            complement(734..1528)
                     /gene="trpA"
                     /locus_tag="AC16FU_02605"
     CDS             complement(734..1528)
                     /gene="trpA"
                     /locus_tag="AC16FU_02605"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR2993296.1"
                     /translation="MNRVWRELRNDRRKALVPFVTCGFPRPQSTVAVVRSFAEAGATF
                     VELGLPFSDPVADGPAIQYSSQVALENGVTVRSVLKTVEQIRKQSNIPRLLMGYANPL
                     VQFGWARFVCAARDAGVDGLLIVDMTLEEAKPVSTLCQKAGLSLVFLISPTTPQQRVK
                     TLDRASSDFSYCLSVTGVTGSRWQLDGDLRGFLARVRQNTKKPFVVGFGLWRAEQVRQ
                     IKSLCDGVVIGSVLVDCYRTEKSERQATQKAARLLKGIGKALNTAG"
     gene            complement(1534..>2683)
                     /gene="trpB"
                     /locus_tag="AC16FU_02610"
     CDS             complement(1534..>2683)
                     /gene="trpB"
                     /locus_tag="AC16FU_02610"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR2993297.1"
                     /translation="KARRDREFQSELRGLLAHYAGRPTPLYLAERLSAKLGLRIYLKR
                     EDLTHTGAHKINNTIGQILLGKQLGKTRIVAETGAGQHGLATATVAAKFGLECTIYMG
                     AADVQRQALNVFKMKLLGAQVIPVTSGSRTLKDAINEAIRDWVTNVRTTHYLLGSTVG
                     PHPYPTIVRDFQSVIGRETKKQIIGQAGRLPDLIFACVGGGSNALGMFYPFLTTKVRL
                     VGVEAAGLGLNTKKHAATLNRGKAGILHGSLSYLLQNGNGQVQVAHSISAGLDYPGVG
                     PEHSFLKESGRVEYTAVTDAEALEAFQILSETEGIIPALEPAHAVAQVMKMAPRLKRN
                     SLVVINLSGRGDKDMDIVRERLHLASAAIHGQNGGWQKRKVSHAGTNR"
BASE COUNT          557 a          814 c          709 g          603 t
ORIGIN      
        1 gtagattttt tgaaatgact ccagagaagt ccggctgaag cgctgccaca gcgttaccgc
       61 ttcgccgtcg cctgattcta atttcttgaa ccaccgccgc ccctcttcag cggtttgcgg
      121 attggcatcg gcttcggtat tgaagcggac gtacagttca tagagatgcc gaatcgggtc
      181 gctggcgagc gcttcttctg aaccccagcg cttgaacgcg gcaatcaggt agccgaactg
      241 cgtcccccag tcgccgaggt gattgatgcc taacgtcggg tggcccaatt tccgatacag
      301 aaggcccagc gcattgccga tgacggttga ccggaggtgc cccacgccga acggcttggc
      361 gatattgggc gaggaaaaat cgatgacgat ggttttgcct ttgccgacgt cggatgagcc
      421 gaacttttca ccggctgaaa aaatttcctt tagcgtttcg tccgtgaacg tcttctgatt
      481 cacccgaaag tttaggtagc cgccgacgac agttacttcc tgtattgcat tcgccgcctt
      541 gaacagagca gccaaatcat tggcaatctt ttccggcgcc tggcgcaact gcttgctcaa
      601 gcggaagcag ggcaaggcgt agtcaccgaa ttggctgtcc ggcggcactt cgatcagttc
      661 ggcaatcgct tccggagcca ggccgagccg gggctcaaga gtcgtaatga tttcgtggac
      721 gaaacggttc atttcaccct gcggtattta atgcttttcc gatgccttta agcaagcgtg
      781 ccgctttctg cgtcgcttgc cgttccgatt tctcggtgcg gtagcaatca accagcacac
      841 tgccaatcac cacgccgtcg cagagcgact tgatctgccg cacctgctcc gcccgccaga
      901 gtccgaagcc aaccacaaac ggtttcttag tgttctgccg cacgcgtgcc aagaagccgc
      961 gcaggtctcc atctaactgc cagcggctgc ccgtcactcc ggtaaccgac agacagtacg
     1021 aaaaatcgct ggaagcgcga tctaatgttt tcacccgttg ctgcggcgtt gtcggggaaa
     1081 tcagaaagac cagggaaagt ccagctttct gacacaaagt gctcactggt tttgcctctt
     1141 ccaaggtcat gtccactatg agcagaccat ctacgccggc atctctggcg gcacagacga
     1201 accttgccca tccaaactgc accagcgggt tggcataccc catcaaaaga cgtggaatat
     1261 tgctctgttt ccgtatctgt tcgaccgtct tcaaaacaga tcgaaccgtc acgccatttt
     1321 ccagcgccac ctgcgaggag tattgaatcg ccggcccatc cgccaccgga tcggagaagg
     1381 gcaaaccgag ttcgacgaaa gtggccccgg cttccgcaaa cgaccggaca acagcaactg
     1441 tggattgtgg gcgtggaaaa ccgcaggtta cgaacggcac gagggctttt ctccggtcgt
     1501 ttcgcaattc cctccaaact ctgttcattc ggttcatctg tttgttcccg cgtgagacac
     1561 ttttctcttt tgccagccgc cattctgccc gtgaatggcg gcactggcta aatgtaaccg
     1621 ctcccgcaca atgtccatat ccttgtcacc gcgcccggag aggttgataa ccacgagcga
     1681 attccgcttc aaccgcggcg ccatcttcat aacctgtgcc accgcgtggg ctggttccag
     1741 ggcgggaata attccttcgg tctcggagag tatttggaac gcctccagcg cttcagcgtc
     1801 ggtaaccgcc gtgtactcca cccgtccgct ctctttcaaa aaactgtgct ccggtcccac
     1861 gccggggtaa tctaaaccgg cagaaattga gtgggccacc tgcacctgcc cgttgccgtt
     1921 ctggagcagg taactcaaac tgccgtggag gatgcccgct ttaccccggt tcaaagtcgc
     1981 cgcgtgtttt ttggtattca atcccaaccc cgccgcctcc accccaacca accgcacttt
     2041 ggtagtaaga aatgggtaaa acatccccaa cgcattggag ccgccgccca cgcaggcaaa
     2101 gatgaggtcg ggtagtcgcc ccgcctgacc gatgatttgc tttttagtct ccctcccaat
     2161 gaccgattgg aaatcccgga caatcgtcgg atatgggtgt gggccgacgg tggaaccgag
     2221 aaggtaatga gtggtgcgca cgttggtcac ccaatcgcgg atcgcttcgt tgatggcatc
     2281 cttcaaagtc cgcgagccgg aagtgaccgg gatgacctgc gctccaagaa gtttcatttt
     2341 gaaaacattg agcgcctgcc gttgcacatc cgccgcgccc atataaatgg tgcactccag
     2401 cccgaatttg gccgccaccg tcgcggtggc cagaccgtgc tgccccgctc cggtctcggc
     2461 aacgatgcgg gtcttcccca actgttttcc cagaagaatc tggccgatcg tattattgat
     2521 tttgtgcgct ccggtgtggg tcaggtcctc gcgcttcagg taaatccgca aaccaagttt
     2581 ggccgagagg cgttccgcta agtagagagg cgttggccga ccggcgtagt gcgccagcaa
     2641 accccgcagt tcgctctgaa attcccgatc gcgcctcgct ttc
//
LOCUS       DBNGZA010000120         1662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2357044, whole genome shotgun sequence.
ACCESSION   DBNGZA010000120 DBNGZA010000000
VERSION     DBNGZA010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2357044"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC16FU_02615"
     CDS             <1..190
                     /locus_tag="AC16FU_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993298.1"
                     /translation="GKDALFVYDVRNRYWTPEKLSLFFARVEPADSLPVFRAGKAVTT
                     FYIFKCSQYLGVGGRTSG"
     gene            316..1290
                     /locus_tag="AC16FU_02620"
     CDS             316..1290
                     /locus_tag="AC16FU_02620"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR2993299.1"
                     /translation="MTTSAPQEAILDRKESQPLGAAVLTTEVTDYPLYARGKVRDIYD
                     LGDRLLFVATDRISAFDVVLPTGIPGKGKILTGLSVFWFDFLKKIVPNHFITADAREY
                     PKPLKKYESILAGRSMLVKKVKRIDLECVVRGYIAGSFWKEYVEARKISNAGNIILHG
                     EVLPDNLKESEILPFPIFTPATKADTGHDENISLAEAQKVVASETVLKLREVSLRLFQ
                     KAADYAQKRGIIIADTKFEFGRLGSQLILIDEVLTPDSSRFWPAQKYEPGRPQESFDK
                     QYVRDYLEEVKWNKKPPAPPLPQEVVRNTQKKYLDAYQKLTGRNLSLE"
     gene            1290..>1662
                     /gene="purH"
                     /locus_tag="AC16FU_02625"
     CDS             1290..>1662
                     /gene="purH"
                     /locus_tag="AC16FU_02625"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744183.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR2993300.1"
                     /translation="MPVAGDAVGIRRALVSVADKTGLQELARVLVSFHVELLSTGGTL
                     AHLRAAGIAAVAVAEYTDAPEILDGRVKTLHPKIQAGILARRDRETDQSEMERLKYPL
                     IDLVVVNLYPFEATIAKENVSS"
BASE COUNT          371 a          485 c          449 g          357 t
ORIGIN      
        1 cggcaaagac gcactcttcg tttacgatgt ccgcaaccgc tactggacgc cggagaaact
       61 ttcgctcttc tttgcgcggg ttgaaccggc tgattcctta cctgttttcc gagcgggaaa
      121 ggcggtcacc acattttaca ttttcaaatg ctctcagtat ctcggagttg gtggtcgaac
      181 gtccggctaa agttccgcag cccgggattg tatcctgggc tcgcggattt ttgttggcag
      241 aaccggtcgc tccattttcc atttgcctcc acctgtatta tcttaggtca aaccagtgaa
      301 aatcggggag ccgacatgac gacaagcgca ccacaagaag caattctgga tcggaaagaa
      361 tcacaaccgc tgggcgccgc cgtcctcact acggaagtta ccgactaccc gctctatgcc
      421 cgcgggaagg tgcgggacat ctacgatctg ggcgaccggc tcctgttcgt cgccaccgac
      481 cgcatttccg cttttgacgt ggtcttgccc accggcattc caggcaaagg gaaaatcctc
      541 accgggcttt cggtcttctg gttcgatttc ttaaaaaaga tcgtcccgaa ccacttcatc
      601 accgccgacg cgcgggaata ccccaagccg ctcaaaaaat atgagtccat cctggctggc
      661 cgttcgatgc tggtcaaaaa ggtcaagcgg atcgacttag agtgcgtggt gcgtggctac
      721 atcgccggtt cgttctggaa ggagtacgtg gaggcccgga aaatttcaaa tgcgggcaat
      781 atcatcctgc acggtgaagt gctgcctgac aatttgaagg aatcggagat cttgcctttt
      841 ccgattttca cccccgccac caaagccgac accggccacg acgagaatat ttcactggcc
      901 gaagcccaga aggtcgtcgc ttctgaaacc gtgctcaagt tgcgcgaagt tagtctgcgg
      961 ctgtttcaga aagccgccga ctacgcgcaa aagcgcggga tcatcatcgc cgacacgaaa
     1021 ttcgagtttg gccgcctggg tagccagttg attctgattg acgaagtgct cacccccgat
     1081 tcctcccgct tctggcccgc tcagaagtac gaacccggtc gcccgcaaga aagtttcgat
     1141 aagcagtacg tgcgcgacta cttggaagag gtcaagtgga acaaaaaacc gcccgcaccg
     1201 cccctcccgc aggaagtggt acggaataca caaaagaaat atctcgacgc gtaccagaaa
     1261 ttgaccggac gaaatctgtc gctggagtaa tgcccgtcgc cggtgatgcc gtggggatcc
     1321 gccgggcgct ggtgagcgtg gcggacaaga ccggtctgca ggagttggca cgcgtcttgg
     1381 tttcttttca cgtcgaactc ctttccaccg gcgggacgct cgcccacctg cgcgcggccg
     1441 gcattgccgc agtagcagtc gccgagtata ccgacgcgcc tgaaattttg gacggccggg
     1501 tgaaaaccct gcaccccaaa attcaggccg ggatcctcgc ccggcgcgat cgggaaaccg
     1561 atcagtcgga aatggaacgt ctgaagtacc ccctgattga tctggtggtg gtcaatctct
     1621 acccctttga agccaccatc gctaaagaaa atgtttcttc gg
//
LOCUS       DBNGZA010000121         3020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2673602, whole genome shotgun sequence.
ACCESSION   DBNGZA010000121 DBNGZA010000000
VERSION     DBNGZA010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2673602"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="AC16FU_02630"
     CDS             complement(<1..1052)
                     /locus_tag="AC16FU_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2993301.1"
                     /translation="MRRVMSFQELPQNFRRNYRLSIANGLFFNAANAFITGTTIIPLF
                     VSRLTASTILIGLAASLETAGWLLPQGLVAAMTLDNVYQKPQYIRVAIVRVIVFAALV
                     TSVFLFGRSYPTLCLVSFFLFFSIYAMGGGSAGVAFMDIVAKTIPHHKRGSLWGWRMA
                     LGGSLAAVMGLVIKKLLAAFSFPVNFGLVFACAWVLIVAGLTSFSFVIEPPGVNRLPR
                     LSIRQGFQKFLEIFRRDQNFKMLYYIRIAAGSYIIAAPFYIVYGINHLGYPPEVAGLF
                     LTAQMIGLVITNPLWAKISNTRSNRLVLFWSAVFSTLPPVVVLLSNLTPLPAALYAAI
                     FFLMGVAEAGLTIGYL"
     gene            1196..1999
                     /locus_tag="AC16FU_02635"
     CDS             1196..1999
                     /locus_tag="AC16FU_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR2993302.1"
                     /translation="MGAFGEQNFAYFGQYQAGEGWFFRLDPRSKLLLVAALILAAFRL
                     TDPLSLGLLAIGLCALFILAQFPVTLLIRQFRTVGWLFAVIFLLYWFFSSGQTLLGAG
                     PIGPSQAGALDGLRWCLKLFVFVFSGLLFTSTTSPLDLVDSLLATARFLGLGFVRRWL
                     EEIGLIFYLAFGFVPLFAREAGEIKKAQMARGASFDGPFYQRLRKSVPLVFPLIVNSF
                     RRAEEKAVALEARGFGLAPEPTQLEPRQFSRNDWWVTVAAVILGVSVVV"
     gene            1992..2741
                     /gene="truA"
                     /locus_tag="AC16FU_02640"
     CDS             1992..2741
                     /gene="truA"
                     /locus_tag="AC16FU_02640"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR2993303.1"
                     /translation="MSERAGKVRNIKLVLEYDGTGFSGWQRQPKRRTVQEEVEKAIEK
                     ITGEKARLTAAGRTDAGVHARKQVANFFSATRLSRETLQRALNALLPKDVRVTKVAQA
                     LLDFDARRRAKSRTYRYVLVQKPTALARFFTWYVFQPLNLTKMQSATRFLLGRHDFSS
                     FCVREADEKSRRCTVAQARWRKAGAGIVFDITADRFLRGMVRSIVGTLVGVGRGKISA
                     AQFRSILRRRDRRKAGPSAPAHGLCLWDVAY"
     gene            2754..>3020
                     /locus_tag="AC16FU_02645"
     CDS             2754..>3020
                     /locus_tag="AC16FU_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HIR2993304.1"
                     /translation="MKLFIDSANIDEIREVASWGILDGVTTNPSLVAKEKAGFEEILR
                     EICRIVNGSISAEVVSVEFDAMMREARQLAKLHKNIVVKIPLIKE"
BASE COUNT          653 a          802 c          946 g          619 t
ORIGIN      
        1 ttcaagtagc cgatcgtcag tcccgcttcg gccacgccca ttaaaaagaa aatggccgcg
       61 tagagcgccg cgggcagggg cgtcagattc gaaagcaaca cgaccaccgg cggcagcgtg
      121 gagaagaccg ccgaccagaa gagcactaaa cgattgctcc gggtgttgct gattttagcc
      181 cagagggggt tggtaatgac caggccgatc atctgcgcgg tcaaaaatag cccggccacc
      241 tccggcgggt aacccaggtg attgatgccg tagacgatgt aaaacggcgc ggcgatgatg
      301 tacgacccgg cggcaatgcg gatgtagtag agcattttga agttctgatc ccgccggaaa
      361 atttccaaaa acttctgaaa tccctgccga atggaaaggc gcgggagccg gtttactccg
      421 ggcggttcga tcacaaaaga aaaggaagta agcccggcga cgatcaagac ccaggcgcag
      481 gcgaagacca ggccgaagtt cacagggaag gagaaggcgg ctaaaagttt tttgatcacg
      541 aggcccatta ctgccgccag actaccgccc aaggccatgc gccagcccca gagactgccg
      601 cgtttgtggt gggggatggt tttggccacg atatccataa aagccacccc ggcggagccg
      661 ccgcccatgg cgtagatgga aaagaaaagg aaaaaagaca caagacagag tgttgggtaa
      721 ctgcggccaa aaagaaaaac ggaggtcacc agggcggcga agacaatgac ccgcacgatg
      781 gccacacgaa tgtactgtgg tttctggtag acgttgtcca aggtcatcgc cgccaccagc
      841 ccctggggca aaagccaccc agcggtttcc agactggcag ccaggccgat cagaatcgtc
      901 gaagctgtca ggcggctgac gaagagcggg atgatggtcg ttccggtgat gaaggcgttg
      961 gccgcattga aaaagaggcc gttggcgatg ctcagacggt agttgcggcg gaaattctgc
     1021 gggagttcct gaaaggacat aaccctgcgc aagaaagcgt cctttccctc agtgtcaagg
     1081 agatgccgtg cataaaagag ggtctttatc ttgaccctcc cccgcaacgc ggatatattg
     1141 acaccgtgga attgatccca cggacaaatg atcttggcaa gacttcctaa tttctatggg
     1201 tgctttcgga gaacaaaatt ttgcctactt cggccagtac caggcgggtg agggctggtt
     1261 tttccggctc gacccgcgga gcaagttgct tctggtcgcg gcgctcatcc tggcggcgtt
     1321 tcgcctcacc gatccgctca gtctgggcct tctggcgatc gggctctgcg ccctgtttat
     1381 cctggcgcaa tttcccgtga cgttgctcat caggcagttc agaaccgtcg gttggctgtt
     1441 cgcggttatc tttcttttgt actggttctt ttcctccggg cagacgctcc tcggcgccgg
     1501 gcccatcggt ccttcgcaag ccggcgcact ggacgggctc cgctggtgcc tgaaactctt
     1561 cgtcttcgtt ttttccggac tgctattcac cagcaccacg tctccgctgg atttggttga
     1621 ttctctcttg gccacggcgc ggtttctggg tctgggtttc gtccgcaggt ggctggagga
     1681 gattggcttg attttttact tagccttcgg cttcgtgccg ctctttgccc gggaggcggg
     1741 tgaaatcaag aaagcgcaga tggcgcgggg cgcgtcattc gacgggccgt tctaccaacg
     1801 cttgcggaaa agtgtgccct tggtgttccc gttgatcgtg aattcgttcc gccgggccga
     1861 ggagaaggcg gtggctttag aagcgcgggg atttggcctc gcaccggagc cgacgcagtt
     1921 ggagccgcgg cagttttccc ggaacgactg gtgggtgaca gttgcggcgg tcatactcgg
     1981 agtaagcgtg gttgtctgag cgagcgggca aagttcggaa tatcaaactg gtgttagagt
     2041 acgacggaac cggcttttcc ggctggcaga ggcaacccaa aagacgaacg gtgcaggagg
     2101 aagtggaaaa ggcgattgaa aaaatcaccg gggagaaggc gcgcctgacc gccgccggtc
     2161 gaaccgacgc cggggtgcac gcccgcaaac aagtggcgaa cttcttttcg gccacccggc
     2221 tcagccggga aacgctccag cgggctctca atgcgctttt gccgaaggat gtgcgggtga
     2281 cgaaagtggc tcaagcactg cttgattttg acgcccggag aagggcgaaa agccgcacct
     2341 atcgatacgt tctcgtacag aagccgactg ctttagcgcg gttttttacg tggtacgttt
     2401 tccagccgct caatttgacg aaaatgcagt cggccactcg ttttcttttg ggacgacacg
     2461 acttttccag tttttgcgtc cgcgaagcgg atgaaaagtc gcggcgctgc acggttgcgc
     2521 aggcacgctg gcgcaaagcg ggagcgggga ttgttttcga catcaccgcc gaccggttcc
     2581 tgcgggggat ggtgcggagc atcgtgggga ctttggtggg cgtcggccgg gggaagatct
     2641 cggccgctca attccgttca attctccgta ggcgcgatcg gcggaaagcc gggccatcgg
     2701 cgccggctca cggtctctgc ctctgggacg tagcttatta gggaggaagc aagatgaagc
     2761 tattcatcga cagcgccaat atagacgaaa tccgggaggt ggcctcctgg ggcattctgg
     2821 acggcgttac caccaatccc tctctggtag ccaaggagaa agccggcttc gaagagattc
     2881 tccgggaaat ctgccggatc gtgaacggct ccatctccgc cgaagtggtg tcagtggagt
     2941 tcgacgccat gatgcgggag gcgcggcagc tggcgaaact ccacaaaaat atcgtggtga
     3001 aaattccatt gataaaagaa
//
LOCUS       DBNGZA010000122         3691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_660121, whole genome shotgun sequence.
ACCESSION   DBNGZA010000122 DBNGZA010000000
VERSION     DBNGZA010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_660121"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="AC16FU_02650"
     CDS             <1..2042
                     /locus_tag="AC16FU_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2993305.1"
                     /translation="ALLPRLGGEVACVVQKESRDAYRVIASSDPTCFGKELGSPFTPH
                     FAEAVRTGRPLVVPGLLETPLGDRIASAVIAPLFFWGEGQGFLVIGSARPDPEYSSTE
                     LAQMDFAARLAAVAATQITAQRAVVTTEDKIAALSDSAAALLDIPREKFWPEVVRQCE
                     GLIPKAGAVLVFQEAHPGQLALRFANVAGTVPEVELAPREGSWSKALAEHRPYLATAP
                     GEVARLWSDLSPETRTRLAGIIGARTVTAEITLPLAAGRRLVADSTLRDSPSGTAGAV
                     LSLLTLSETTRTGEPTPPFSAVDFHLAKAFACIVSALWVRFSAPAAAGAPITGDLAAA
                     LNDFNNLLTSVLGNAQMLHAQVDHVPGESRQIFSERLEHIAQAAGAAGDLLRSLPSAG
                     SAAGLPSEAAVDLTALVRETLTKERTESLESGSIFAPGRAAIHPELSSVPPVTLDPGK
                     FKAFLGEIFKRIASRPGESVTVKTDADQKYVYLRLWQKGEPPAAFGGVPSQSMEAGTA
                     RDVPVDLDLVRSFRGEASYIEDETSGRALVLRFPIARAAEKSEAEHPARTLSILAIDD
                     QPLIRDLLVAMGANLGHTVRVAESGPAGVALFRQQRFDLVITDLSMPGASGWEVSRTL
                     KKIAPDVPIVLVTGWGHSVEGLDLKALGIDYVLPKPFRLEQFAELVAQVAAQTAS"
     gene            2401..2898
                     /locus_tag="AC16FU_02655"
     CDS             2401..2898
                     /locus_tag="AC16FU_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2993306.1"
                     /translation="MSGKQICQKLSRLQKEGAGFQELLTGAVELLHHSNPRFHWTGIY
                     ELFPDNVLRLGPFIGAPTDHVFIAVGQGVCGTAVAEKRNMNVPDVSKAANYLACSTAT
                     MSELVVLIRNGETIFAQIDIDSHEYDAFDPDTVKTVEQVADWLAGAYRQRLPVSGPKG
                     SLTKA"
     gene            2918..>3691
                     /locus_tag="AC16FU_02660"
     CDS             2918..>3691
                     /locus_tag="AC16FU_02660"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR2993307.1"
                     /translation="MGTLLKTDSDQVKVIEALRHKVEEYERWFRSLDNQVRVLERERQ
                     KFSAVVNQTDAGFFVVDSSLKVIWANDVFTRRFGAALHGGVTCSQVLCCRSERCASCP
                     ATAAFRGGAVAHHEIRLQLDGQPHQLYATAMPIKSPEGKIDETIVMLQDITDLEILRR
                     SQAALKASEARFRSIFESAGAGMATVGADGRYLQANPAFCNFLGYTEAELQRLTLFDV
                     THSDDLEMTRQMLSEVAAGSRRIIDKEKRYVRKDGQTVWG"
BASE COUNT          693 a         1149 c         1156 g          693 t
ORIGIN      
        1 ctgcgctttt gccgcgcctg gggggcgaag tggcctgcgt ggttcaaaag gaatcgagag
       61 atgcctacag agttatcgct tccagtgatc cgacctgttt tgggaaagag ctcggttctc
      121 ccttcacccc ccacttcgcg gaggcggtac gaacgggccg tccgctggtg gttcccggcc
      181 tgctggaaac gcctttgggt gaccgcattg cgtccgccgt gattgcgccg cttttcttct
      241 ggggcgaggg ccaggggttt ttggtcatcg gttcggcccg gcccgatccg gagtacagtt
      301 cgacggaact ggcccagatg gatttcgccg cgcggctggc tgccgtggcg gccacgcaga
      361 tcaccgccca gcgggccgtc gttaccaccg aagacaagat tgccgctttg agcgacagcg
      421 cggcagcact gctcgatatc cccagagaaa agttctggcc agaagtagtc cggcagtgcg
      481 aaggtctcat tccaaaagcc ggggctgttt tggtctttca agaagcccat ccgggtcagc
      541 tggcgttgcg tttcgccaat gtcgcgggaa cggtaccgga agtagagctg gcgccccggg
      601 aagggtcctg gagcaaagcg ctggccgagc accggccgta tctcgccacc gccccggggg
      661 aagtggcccg cctctggagc gatctctccc cggaaacgcg gacgcgcctc gccggaataa
      721 tcggagcgag gacggtgaca gccgaaatca ccctgccttt ggccgccggt cgaagactcg
      781 tcgcagactc gactcttcga gactctccga gcggaacagc gggcgccgtc ctgtcgcttt
      841 taacgctgtc ggaaactacc cgtaccgggg aaccaactcc gcccttcagt gcggtggatt
      901 tccatctggc caaggcgttc gcctgcatcg tgtctgcgct ctgggtgcga ttttcggcgc
      961 cggccgccgc cggggcgccg atcaccggtg acctggccgc cgcgctgaac gacttcaaca
     1021 acctcctcac cagcgttctg gggaatgcgc aaatgctcca cgcgcaggtt gaccacgtgc
     1081 cgggggagag ccggcagatt ttttccgagc gcttggagca tatcgcgcag gcggccggcg
     1141 ccgcgggaga tctactgcgg agcctgccct cggccggcag tgccgccggg ttgccgtccg
     1201 aagccgccgt cgatttaacc gctctggtgc gggaaacctt gaccaaagag cggacggagt
     1261 ctctcgagag cggttcgata tttgcgcccg gccgggcagc cattcatccg gagttgtcca
     1321 gcgtgccgcc ggtgacgctc gatccgggga agttcaaagc cttcctgggt gagattttca
     1381 aaagaatcgc ttcccggccc ggcgaatcgg tcacggtgaa aaccgatgcg gatcaaaagt
     1441 acgtctacct gcggctctgg caaaaggggg agccgccggc agccttcggc ggcgttccca
     1501 gccaatcgat ggaagcgggc accgcgcgcg acgtccccgt cgatctcgat ctggtgcgtt
     1561 catttcgcgg tgaagcgagt tacatcgaag acgaaacctc cggccgggca ctggtgctgc
     1621 ggttccccat tgcccgggcg gccgaaaagt cagaggccga acaccctgcc cgcacccttt
     1681 ccattctcgc catcgacgac cagccgctca tccgcgacct tttagtcgcc atgggcgcca
     1741 atttgggcca cacggtcagg gtggccgaaa gcggcccggc gggcgtcgcc cttttccggc
     1801 agcagcgctt tgatctggtc attaccgacc tctccatgcc cggcgcctcc ggctgggaag
     1861 tctcccgcac cttgaaaaaa atcgcgcccg acgtgccgat tgttttggtc accggctggg
     1921 ggcactcggt ggagggtttg gatctgaaag cgctggggat cgattacgtc ctccccaaac
     1981 cgttccgctt ggaacagttc gccgagcttg tggcgcaggt agcggcccaa accgcgtcgt
     2041 agccgcattg gcaggccaca ggggcctgtt caaaaatctt tcaaataaca tttcgataag
     2101 gcgtttctcg cattgaattt ttgcaggctg aagcctcggt cagacggggg gcctgactcc
     2161 cggatatgtt cctgacgcta tctccctcgc gcacatcaag cgaaacccga atcagccgaa
     2221 aatattaaaa gaacccctct ggaggttcgc aaggagagcg tttcgtcagc gtgcctccgt
     2281 tctccgcaaa ccacgcaccg agggagtgga agtatgttgc aggattaggg agcaccgggg
     2341 accgggaggc actgtggctg aggcagagtt ttttcccagg gaatcgacgg gagcgcgact
     2401 gtgtccggca agcagatctg ccagaaactt agccgattgc aaaaagaggg tgccggtttc
     2461 caggagctct taaccggcgc tgtggaattg ctgcaccact ccaacccgcg cttccactgg
     2521 acgggcattt acgaactttt cccggacaac gtcctgcggc tgggcccctt catcggcgcg
     2581 cccaccgacc acgtcttcat cgcggtgggg cagggggtct gcgggacggc ggtggcggag
     2641 aaacgcaata tgaacgtgcc ggacgtctcc aaggccgcta attacctggc ctgttcgacg
     2701 gcaacgatgt cggagctggt ggtgctgatc cgcaacggcg agaccatttt tgcgcaaatc
     2761 gatatcgaca gccacgagta cgacgccttc gacccggaca ccgtcaaaac ggtcgagcag
     2821 gtcgccgact ggttagcggg tgcttatagg cagcgtctcc ctgtcagtgg gcccaaaggt
     2881 tctttaacca aagcgtaacg tcgcaggcgg acaccccgtg ggaactctac tcaagacaga
     2941 ttccgaccag gtcaaagtca tcgaggcgct ccggcacaaa gtcgaagagt acgagcgctg
     3001 gttccgatcg ctcgataatc aggtgcgggt gctggaacgc gaacggcaga agttttcggc
     3061 ggtggtcaac cagaccgacg ccggtttttt cgtggtcgat tcctccctga aggtgatctg
     3121 ggccaacgat gttttcaccc ggcgcttcgg tgccgcgctc cacggcgggg tgacctgcag
     3181 ccaggtgctc tgctgccgga gcgaacgttg cgcctcgtgt ccggcgaccg ccgccttccg
     3241 gggcggggcc gtggcgcacc acgagatccg tctgcaactg gacggccagc cgcaccagct
     3301 gtacgccacc gccatgccga tcaaatcccc cgaggggaaa atcgacgaaa ccatcgtcat
     3361 gctccaggat atcaccgacc tcgagatctt gcggcgttcg caggcggcgc tcaaggcgag
     3421 cgaagcgcgc ttccggtcga ttttcgagag tgccggcgcc ggcatggcca ccgtcggagc
     3481 ggacggccgc tacctgcagg cgaatcccgc cttctgcaac ttcctggggt acaccgaagc
     3541 cgaactgcaa cggctgaccc tcttcgacgt cacccattcc gacgatctgg agatgacccg
     3601 gcaaatgctt tccgaagtgg ctgcgggcag ccgccggatt atcgacaagg aaaaacgcta
     3661 cgtccggaaa gatggccaaa ccgtctgggg g
//
LOCUS       DBNGZA010000123         5344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1825829, whole genome shotgun sequence.
ACCESSION   DBNGZA010000123 DBNGZA010000000
VERSION     DBNGZA010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1825829"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2640
                     /locus_tag="AC16FU_02665"
     CDS             <1..2640
                     /locus_tag="AC16FU_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2993308.1"
                     /translation="QKELMDLNTAQIWTVKPLLKTVPGVVEVSMLGGYMRQFHVLPRL
                     DRLRKYGVSLKEVFRAVAENNSVAAGNILDLHSEQNTIRGVGLIKNAQDIETIVVKNH
                     DGTPIFVRDLADVQEGYETRRGAAVENGEGEAVTGLVVMIKDGNAREIVQKIKDKVAE
                     INAAGILPDGMKIVPYYDRTELVDKSTATVEKALLEGILLLVVVLYLFLRNFRSALVV
                     SLALPLAALATFIFMRLLGLSANLMSLGGLAISMGMVIDGAIIQVENVLRHSSENDSQ
                     KTHFQLVLDSVLEVQKPSLFGVLIIALTFLPILSLQGMEGKLFSPLAKTVILALLSSL
                     VLSVLVIPVLCLFLIRPQPHKTTGLAEFLKSRYARALDFCLRRKRLVVSAAFLFLALS
                     LAVVPFLGTEFIPHMDEGNVLLQVLRLPSISLEESNKTDLWVQRRLMQFPEVEKIVSN
                     TGTDEAATDVMGQQLSELNISLKPEKGWKTAKTKEELIEKMRAALAVLPGMAYNFTQQ
                     IAMRVDELVTGVKSPVAVKIFGEDFDILSVKAQETARVISAVKGTVDLRVGQQAGQPT
                     LSIEADRRKMARFGINVADIQEIIEIAIGGKVATEVLEGDRRTEVVVRFPEELRNSVA
                     AIENIPVQSPSGVPVPLGQLVNFQWEENPAAIEREGGKRRVVIECNAAGRDIGGFVRE
                     CREKVKRLVKLPPGYYLTWSGAFENQQRANRRLAVVMPLTVLLVFFLLYTTFGQARQA
                     VLVLLNLPFALTGGVLALLVSGLYLSVPASVGFIALFGVAVLNGVVLISYINQLRQRG
                     TPLEDAIRTGCEKRLRPVSMTALVTMLGLTPLIFATGVGSEVQKPLAVVVVGGLLTST
                     TLTLLVLPVLYQWFERKKEGRRP"
     gene            2640..3413
                     /locus_tag="AC16FU_02670"
     CDS             2640..3413
                     /locus_tag="AC16FU_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HIR2993309.1"
                     /translation="MTPTALAEQIEKHVRPETFPLAIRMLTVGEAPPEKARRPASDLK
                     AKIAVCQGFGISRRYGWTLAIGEEDISCPLTKVAFGFKKEPAFFTQGFCCEGMYTKTK
                     EAGAKTESQLPKFEYGKYRFVLVGPLARAAYEPHVVLLYANPAQVLRLLTASLFGRGG
                     YHTAKFSGRIDCADEVIRTMLTGEPQVILPCYGDRIFGLVQDHEMAFAFPGSFADELV
                     AGLEGTHKGGIRYPIPQFMRFTGQYPEKYQVLEQYFDKE"
     gene            3400..4476
                     /locus_tag="AC16FU_02675"
     CDS             3400..4476
                     /locus_tag="AC16FU_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR2993310.1"
                     /translation="MIKNKTPKKSPARGRRVPTAASLGSSLTHRERITNLIAGKAVDR
                     PPVTFWRHFYHRENAPETLAEALATFQKKFDWDLVKINPRASYHIEDWGAKYRFSKDE
                     FTKPGRLEYPIHVATDWENLEVLTPTRGTLGEQLQAIRLVAKRIGPTVPKVMTVFTPL
                     SIAGDLVGQDEHLVRYIREYPLLLKPALEVIAETFERFAVECLSAGADGVFLATTEWA
                     SGELLSPEEYQEFDLPYARRVAEAVKNAGGLVVLHVCKSQNFLHLFSAFPADIVNWDA
                     ADSTNLKLKQGATLLKRPVLGGFDYKSELTSLSPKKATEVAQKRLQENRSIRFILGPG
                     CAIPPETPEAVLFAIRRAVEGKIK"
     gene            4730..>5344
                     /locus_tag="AC16FU_02680"
     CDS             4730..>5344
                     /locus_tag="AC16FU_02680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2993311.1"
                     /translation="MKSKADVVVIGGGVIGAAVAYNLAKRGLKEVVVLEKEPFLASGS
                     TGKAAGGIRAQFSTPGNILFSMRSEELFACFPEEMDSPAQFEQVGYLFLVSDEKTWEI
                     YKQQAELQQKLGLPVQLLTPEEALQLAPFVRMSDLVGATFCGRDGLGDPGDFSQGYIT
                     QARRLGVEFALEAEVTGIEVTGGKVGGIISQKGRIATQVAVNAAG"
BASE COUNT         1121 a         1426 c         1579 g         1218 t
ORIGIN      
        1 caaaaagagc tgatggattt gaacacggcg cagatttgga cggtcaaacc gcttttaaaa
       61 accgtgcctg gagtggtgga ggtgagcatg ttgggcggct acatgcgcca gttccacgtt
      121 cttccccggc tggaccgcct gcgcaaatat ggtgtttcgt taaaagaagt ttttcgagcg
      181 gtcgcagaaa acaactcggt ggcggctgga aatatattgg acctccattc cgaacagaat
      241 accatccgcg gcgtcggatt aatcaagaac gcccaagaca tcgaaactat cgtggttaaa
      301 aaccacgacg ggacgccgat ttttgtgcgg gatctggcgg acgtgcagga agggtacgaa
      361 acccggcggg gggcggcagt tgaaaatggg gaaggggagg cagtcaccgg cttggtggtg
      421 atgatcaagg acggcaatgc ccgcgagatc gtccaaaaaa taaaagacaa agtggcagaa
      481 attaacgccg ccggcatcct gccggacgga atgaaaatcg tgccgtatta cgaccgcacg
      541 gagctggtgg ataagagcac cgccacggtg gaaaaggcac tcctggaagg aattttgttg
      601 ttggtggtcg ttctctatct gttcctacgc aacttccgca gcgctttagt ggtctctttg
      661 gctttgcccc tggcggcttt agccaccttt atttttatgc gtcttttggg actttcggcc
      721 aatttaatgt ctctgggcgg gctggcgatt tccatgggaa tggttattga tggagccatt
      781 attcaggtcg aaaacgtttt gcggcattcg agtgaaaacg actctcagaa aacccatttt
      841 cagctggttt tggactcggt cctggaagta caaaaaccga gcttattcgg agttttgatt
      901 atcgctttga cttttttgcc gattctatcg ctccagggaa tggaaggaaa acttttctcc
      961 cccctggcaa agacagtgat cttggccctg ctttcctcgc tggtgctttc ggttttggtc
     1021 atcccggtgc tttgcctttt cctcatccgc ccccagcctc ataaaaccac cggcctagcg
     1081 gagtttttaa aaagccgcta cgctcgggct ttggattttt gcctgcgccg taagcgcttg
     1141 gtggtgtccg ctgccttttt gtttttggct ctttctctgg cggtagtccc ctttttgggg
     1201 accgagttca tccctcatat ggatgaaggc aacgttttgc tccaagtttt gcggcttccc
     1261 agcatatcgc tggaagaatc gaataaaacc gacctgtggg tgcagaggcg cttgatgcag
     1321 tttcccgagg tggaaaagat cgtttccaat accggcacgg atgaggccgc caccgatgtg
     1381 atgggccagc agctcagcga gctcaacatt tctttgaaac ctgaaaaagg atggaaaacc
     1441 gccaaaacca aagaggagtt gatcgaaaaa atgcgggcgg cactggcggt gcttcccggc
     1501 atggcctaca atttcaccca gcagatagcg atgcgggtgg atgagctggt cacgggggtg
     1561 aaatcaccgg tggcggttaa aatcttcggt gaggattttg acatcctctc ggtcaaagcg
     1621 caggaaaccg cacgggttat ttctgccgtc aaggggacag tcgatttgcg ggtgggacag
     1681 caagccggac agcctaccct ctcgattgaa gccgatcggc ggaagatggc ccgtttcggc
     1741 atcaatgtgg cggatatcca ggaaatcatt gaaattgcca ttggcggcaa ggtggccacc
     1801 gaagttttgg aaggggaccg ccgcacggaa gtcgtggttc gttttcccga agagttgcgc
     1861 aattcggtgg cggcaattga aaatattccg gtccagtccc cctccggggt gccggttccg
     1921 ctgggccaac tggtgaattt ccaatgggaa gaaaatccgg ccgccattga acgcgagggc
     1981 ggcaagcgcc gggtggtcat cgagtgcaat gccgccgggc gggacatcgg cggcttcgtg
     2041 cgggagtgcc gcgaaaaagt taaaaggctg gtcaagctgc cgcccgggta ctacctcacc
     2101 tggagcggag cttttgaaaa tcagcaaagg gccaaccgcc gcctggcagt agtaatgcct
     2161 ttgacagttt tattggtctt ctttcttctc tataccacct tcggacaggc ccggcaggcg
     2221 gtgctcgtgc ttttaaatct cccattcgcc ttaaccggcg gggtcttggc cctcttggtc
     2281 tccggcctct atctttccgt tccagcgtcg gtcggcttta tcgccctttt cggagtggcg
     2341 gtcttgaacg gcgtcgtgtt aatttcctat attaaccaac tgcgccagcg aggtacgcct
     2401 ttggaggacg cgattagaac cggttgtgaa aaacggttgc ggccggtttc aatgactgct
     2461 ctggtgacca tgctcgggct gacaccccta atttttgcca cgggtgtcgg ctcggaagtc
     2521 cagaaacctc tggcagtggt ggtggtcgga ggccttttga cttccaccac tttaacttta
     2581 ctcgtgctgc cggtcttgta tcaatggttt gaaagaaaga aagaaggtcg gcgaccgtga
     2641 tgactcccac agcgttggct gaacaaatcg aaaagcacgt ccggcccgaa acgtttccgc
     2701 tggcaatccg gatgctgaca gtcggcgaag cgccgccgga aaaagcccgc cgtcccgcca
     2761 gtgacctgaa ggcaaaaatc gctgtctgcc agggattcgg catctcccgg cgctacggct
     2821 ggactttggc cattggtgaa gaggacatct cctgcccctt gaccaaagtc gccttcggct
     2881 tcaaaaaaga gccggctttt ttcacgcagg gtttctgctg tgaggggatg tacacgaaaa
     2941 ccaaagaagc gggggcgaaa accgaaagcc agttgcccaa attcgagtac gggaagtacc
     3001 gtttcgtctt ggtcgggccg ctggcccgcg ccgcctatga gccccacgtc gttctgctct
     3061 acgccaatcc ggcccaggtc ttgcggcttt tgaccgcgtc gctctttggg cgcggcggct
     3121 accacacggc aaagttctcc ggccggatcg actgcgcgga tgaagtgatc cgcacgatgc
     3181 tcaccgggga accacaggta attttgcctt gctacggcga ccggattttc ggtctggtgc
     3241 aggatcacga gatggccttc gcctttcccg gatcgtttgc ggatgagttg gtggccgggc
     3301 tggaaggcac gcataaaggc ggtatccgct acccgattcc gcagttcatg cgcttcaccg
     3361 gccagtaccc ggagaagtat caggtcttag agcagtactt tgataaagaa taagactccc
     3421 aagaaatcac ctgctcgggg gcgtcgtgtt cccaccgctg cctctctcgg ctcttccctg
     3481 acccaccgcg agcgcattac caatctcatc gctggcaaag cagttgaccg cccgccggtc
     3541 accttctggc ggcattttta ccacagggaa aacgctccgg agaccctggc cgaagcgctg
     3601 gcgacgtttc agaaaaaatt tgactgggat ctggtgaaga ttaacccgcg cgcctcctat
     3661 cacatcgaag actggggcgc gaagtaccgt ttttcgaagg atgaattcac caaaccgggg
     3721 cggctggagt acccgattca cgttgccacc gactgggaaa acttagaggt actcacgccc
     3781 acgcggggta ctctgggaga acagctacag gcgatccgtc tggttgccaa aagaattggg
     3841 ccgaccgtgc caaaagtgat gaccgtattc acgccgcttt cgattgcggg ggatttagtg
     3901 ggccaggatg agcatctcgt caggtacatt cgcgagtacc cgcttttgct caaaccggcg
     3961 ctggaggtga ttgccgaaac gtttgagcgc ttcgccgtcg aatgcctctc cgctggggcc
     4021 gacggcgtct ttttggctac gacggagtgg gcctccggcg aacttttgtc gcccgaagag
     4081 taccaggagt tcgatttgcc ctacgcccgg cgggtggcgg aggcagtcaa aaatgcaggc
     4141 ggactggtgg tgctccacgt ctgcaagagc cagaatttcc tccacctctt ttccgctttt
     4201 ccggcggata tcgtcaactg ggacgctgcc gactccacca atttgaaact caagcaggga
     4261 gcgacccttt tgaagcgccc cgtcttgggc ggttttgact ataaatccga actgacgagt
     4321 ctctctccaa aaaaggccac ggaagtcgcg cagaaacggc tgcaggaaaa tcggtcgatc
     4381 cgtttcattt tgggccccgg ctgcgccatc ccgccggaga cgccagaggc ggttcttttc
     4441 gccatccgcc gggcggttga ggggaagatc aagtagtgtg tccgtaacct gcggtgcaat
     4501 cgacattggc tctaatttgc cgaaagcctt cgatggcacg gatcaagttc ttttggaaga
     4561 agtagggctg ccttggccgc ttgttttgtc gtctgaagtc gtcctcccac agcggttttc
     4621 ctcagactgt cgcggtataa cggcctattc ctgtaaaaaa cgccgctgac ggcacggtcc
     4681 tgcttgactg tggccgccgg gagaaattga ttactcctcg gagcgggaga tgaagagtaa
     4741 agccgacgta gtggtcatcg ggggtggagt gatcggggcg gccgtggcct acaatctggc
     4801 caagcggggg ctcaaggagg tcgtcgtcct cgaaaaggaa ccatttctcg cttccggttc
     4861 cactggcaag gccgccggcg gtatccgggc gcaattctcc accccgggaa acattctttt
     4921 ctccatgcgc tctgaggagt tattcgcttg cttcccggaa gaaatggact caccggccca
     4981 gttcgagcag gtgggttatc tctttttggt ctccgacgaa aagacgtggg aaatctataa
     5041 acagcaggcg gaactccagc agaaattggg cctgccggtc cagctcctca cgcccgagga
     5101 ggcgttgcag ctcgcaccct ttgtgcggat gagtgatctg gtgggtgcga ccttctgcgg
     5161 cagggacggt ttgggggatc cgggcgactt cagccagggg tatatcaccc aggcccggcg
     5221 gctgggggtt gaatttgccc tcgaggccga ggtcaccggc atcgaggtca caggcgggaa
     5281 ggtgggcggg atcatttccc aaaaggggag aatcgctaca caagtggcgg tcaatgccgc
     5341 cggc
//
LOCUS       DBNGZA010000124        14514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1507587, whole genome shotgun sequence.
ACCESSION   DBNGZA010000124 DBNGZA010000000
VERSION     DBNGZA010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1507587"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..755)
                     /locus_tag="AC16FU_02685"
     CDS             complement(66..755)
                     /locus_tag="AC16FU_02685"
                     /inference="COORDINATES: protein motif:HMM:NF026253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR2993312.1"
                     /translation="MDKTGKMVIKPQFYSAYDFSEGLAMVFTGHWGYIDKTGKMVIEP
                     QFDDAWKFSEGLAPVQKIYGKEWGFIDRTGKMIIPSQFDKAESFSEGLALVKINWKRG
                     YIDKTGKTLIPPQFDTAMSFSEGLAAVRIDGKWGFIDKLGRYVVKPKFHDADNFSEGL
                     ARVQINSKWAFIDKTGKEIIKPKFDFAWNFSEELAFVEIDKKWGCIDKTGRVKSHSSH
                     QIDMLVAWQDD"
     gene            complement(894..1544)
                     /locus_tag="AC16FU_02690"
     CDS             complement(894..1544)
                     /locus_tag="AC16FU_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993313.1"
                     /translation="MNLERLSTVGYLFIGLGVAILLWGLFRKLKDSTRKPPARQDESS
                     GPSIVGPILLAALLVGLGQLLVWLVGEHENFYPPKPDRPVASLKLVIPKSGDPYLEVQ
                     ELYKSGRYRLLLSKGPGELCRITANAIKWRKEFSFLGLKEYLRLTEVVVRDTADSAGA
                     RPAKLVLSEEAQEFASFLRSSNLPVVNSKEIASAPFPLLPDHEYQVFLQNDKFEIE"
     gene            complement(1680..2489)
                     /locus_tag="AC16FU_02695"
     CDS             complement(1680..2489)
                     /locus_tag="AC16FU_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR2993314.1"
                     /translation="MRKIRHMTFTVFSEDDQKALSIRLPSIIFYLIIGLLIATVIGLV
                     ISVLYSSRLAAKTSLLESVQIENQKLRNYTSKITLLEKELFDNRVLTVKLAKMAGIEL
                     PMLQDSSKSPTNVSMPESSKVVVSETISSSVASVLPPQKEALPTGLPASGTRLPRSLL
                     SGSDLLLPGTFLAATARVSVRATAEGTVEFSGRDSSLGNLVVVSHAQGVKTRYGRLGE
                     LKVSRGQKVNKGQTLGYTTPPDSTGKSIFYYEVQGTTNPPKRSDLSASSDS"
     gene            complement(2542..3393)
                     /locus_tag="AC16FU_02700"
     CDS             complement(2542..3393)
                     /locus_tag="AC16FU_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR2993315.1"
                     /translation="MTQKIALGKGLSALIPEGETGMEGESIQPILLAKIQPNPDQPRQ
                     AFDPERLAELANSLKDQGVLQPIIVRPVGENYQVVAGERRVRAAQLAGFESIPALVRE
                     NVSKEMALELALIENLQREDLNPIEEARAYKQLATEFGLSQEAIAGKVGKERSSVANA
                     LRLLNLPQGIQTKIAEGELSPGQARLLLRLSDPQRQNQLAERIVQGSISVRELERLIE
                     SPRPIRRRLKLKRPAALFDTIEQALKRFFGTNVILRRGKKRGKIVIEFYSDDDLNRIL
                     QLLKIAV"
     gene            complement(3414..4253)
                     /locus_tag="AC16FU_02705"
     CDS             complement(3414..4253)
                     /locus_tag="AC16FU_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2993316.1"
                     /translation="MGKVIAVVNQKGGVGKTTTAINLSACLAAAERNTLLIDIDPQAN
                     ATSGIGLDKSKIKTTIYEALLEELPLREIILKSELAYLDVAPSHTRLVGAEVELVNAI
                     GREKRLTTAVSPIRNSYDFIFVDCPPSLGLLTVNTLTAADSVLIPIQCEYYALEGLGQ
                     LLNTIRLIQKHLNPGLAIEGVLLTMYDSRLNLSKQVADEARKFFTDKVYQTIISRNVR
                     LSESPSFGKPIILYDILSTGAENYIALAKEFLERNGADPQALEPAGVTRTILSELEPA
                     AVK"
     gene            complement(4365..5030)
                     /gene="rsmG"
                     /locus_tag="AC16FU_02710"
     CDS             complement(4365..5030)
                     /gene="rsmG"
                     /locus_tag="AC16FU_02710"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR2993317.1"
                     /translation="MKHCPSPKVEQTKIDLPSALKLINLEPSNLQLSQFYIYFDLVLK
                     HWKKFDLVAPSQANQFGLARQTVDSLSVLRAIDFPQNAKIADVGSGAGFPGIPLAIVR
                     PDLQLTLIDAKPKKSLFLQECRTVLKLSFQIIQSDVRRVTEQFDFTLYKAFADLKMVV
                     RLSRRLLVSGGVAVAYKTKNYPSELKKLKFFTGTARLESAENVPVPELNLNTCFLIFR
                     KLD"
     gene            complement(5077..7047)
                     /gene="mnmG"
                     /locus_tag="AC16FU_02715"
     CDS             complement(5077..7047)
                     /gene="mnmG"
                     /locus_tag="AC16FU_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR2993318.1"
                     /translation="MSNLTYKENYDVIVVGAGHAGCEAALVCARMGLRTSLFTQNRDT
                     IAQMSCNPAIGGQAKGQLVKEIDALGGEMGWVADQTGVQFKILNKSKGPAVWSSRSQN
                     DRLLYKLCMRQVVTSQAGLEIVEDGAIDILTTGGRATGVVGSSGNRYKAQAVVLCSGT
                     FLNGLIHIGKKNFPAGRVGESPALYLSENLQKLGFETGRLKTGTPPRLSHKSIDYSKT
                     ELQPGDDPPVPFSLRTDKIKNPQLFCWLTYTSAETHQIIRDNIHQSPMYSGQIKGIGP
                     RYCPSVEDKIFRFADKPRHQIFFEPEGYETDVVYINGFSTSLPEEVQFAALKTIPGLE
                     QVEMLKPGYAVEYDFFPPTQLKHSLETKLIENLFFAGQINGTSGYEEAAAQGIMAGIN
                     AALKIKGEEPFVLDRSEAYIGVLIDDLVTKNPLEPYRMFTSRVEYRLAIREDNAADRL
                     MPYGYKLSLVPKRLYQKYQERRGEIEREKDRLQKSRYRRGELFSLLMNGNRTAEREGE
                     HDDPLVIEAEDRPPDIARTNDNGKSPNPDQKISLFTFLRDPRVNYRHIREIDSESVCV
                     RPQIAQQVELEVKYEGYILRQEKEIAKFKKTEGMRIPERINYLSLKGFKREAQEKLQR
                     IRPATVGQASRISGVTPGDIAVLMVHLKQLES"
     gene            7198..8241
                     /locus_tag="AC16FU_02720"
     CDS             7198..8241
                     /locus_tag="AC16FU_02720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003324180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2993319.1"
                     /translation="MADGSKNLNEICDITIIGAGPVGLFAAYYAGLRALRAKVIDSLP
                     KVGGQLTSLYPKKNIYDVAGFPKILAEDLVQNLLVQALQYRPELCLEQRIQKLEYVDG
                     NLIQLSDENGIRHLTRTVIISAGTGSLVPRRLELPGVEKYLDHGLTYVVLDPEEYRGK
                     RVLIVGGGDTALDWALTLKDIAKETTLIHRSGKFAGHEDSVRKLFASHVRTHIHTEIK
                     TIHGDSRVAGATITTREKNEEITLDLDAIIVCIGFTPNLGAIKEWGLAIEKNSIQVNS
                     RMETNLPAVYAVGDVVDYPGKVKLIVTGFGDAAVAVCHAAHRINPQLKVFPGYSTNFV
                     EKLQAKVQTSVGG"
     gene            8289..8987
                     /locus_tag="AC16FU_02725"
     CDS             8289..8987
                     /locus_tag="AC16FU_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR2993320.1"
                     /translation="MIKIRKADERGHADYGWLSTYHTFSFDTYYDAAYMGFRSLRVIN
                     EDRVQPGNGFPSHFHRDMEIISYVLEGALEHRDSLGTGSIIRPGDVQRMSAGTGVTHS
                     EYNPSQTDIVHFLQIWILPERKGLKPSYEQKRFSDEEKRGTLRLIASREGRDGSVSIH
                     QNASVYATSLDIGQELVHPLKPGRHAWAQVVRGRATFNDRELKQGDGAAASEEKALKL
                     ASREGAEVLVFDLA"
     gene            9043..11292
                     /locus_tag="AC16FU_02730"
     CDS             9043..11292
                     /locus_tag="AC16FU_02730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR2993321.1"
                     /translation="MTQPQEPQKTIDLAISGMTCASCVSTVEKALKSVSGVTQASVNL
                     ATERALVTYRPGVATLEQMFKAIADSGYKARELSAETRDEREELLKEELTTLKKKLFF
                     SLIAAALLMLLGFQMYLPGLKAFPRSLIPWLGLVLATPVQFWAGARFYRGFWAQLKHG
                     RADMNTLIAIGTSAAYFYSVAVTFSPSGFTSAGHRAEVYYETAAAIIALILLGRYLET
                     QAKGRTSAAIKKLLGLAPKTARVRRDGSEMDLPIDQVFVDDTVIVRPGERIPVDGQVI
                     EGYSAIDESMVTGESLPVEKKIGDRVIGGTINKTGSFVFRATKVGKDTLLAQIIHLVE
                     KAQGSKAPIQKLADRVAAVFVPIVLVIAAITFAVWFLFGPEPKLTRALLNCVAVLIIA
                     CPCAMGLATPTAIMVGTGRGAEMGILIKDAGALEKAQKLDTVVFDKTGTVTLGKPAVT
                     AVFPLNGFAPEQVVRWAASAEKRSEHPLAEAVTRYAEEKKFALSEPTAFTAEPGFGLV
                     ATVEAKRILVGNEALLRRERISLDGVSAKQFAANSGASFLWVAVDGRVAGALTVADTL
                     KPNAVSAVVRLKSLGLEPVLASGDREETTVEIAKKLGIVAYYAQILPQEKGDLVRLLQ
                     QRGKKVAMVGDGINDAVALAQADVGIAMGTGTDVAIETADITLVKGDLSGVARAIALA
                     RKTVKTIRWNLFWAFIYNIIGIPIAAGALYPFFGWQLNPMLAAAAMAFSSVFVVTNSL
                     RLRKFQEGV"
     gene            11403..12209
                     /locus_tag="AC16FU_02735"
     CDS             11403..12209
                     /locus_tag="AC16FU_02735"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR2993322.1"
                     /translation="MTETIQFLIRYGYIVLFISVFAEQIGLPLPAIPLLLAAGALAGL
                     GRLSFALVVVLAVAGSLVADFIWYEIGRRRGGRVLNFLCRISLEPDSCVRRTENFFAR
                     YGARSLLLAKFIPGFSVVAPPLAGIFGMRLGRFLSFTALGALIWAGTFTGVGYLFSSQ
                     LEQVATYAGQFGASLVAVLVGGLALYLAWKYLQRRHFLAQLRVARITPEELKQKLDAG
                     DEMIIVDLRQSLDFEAEPHTIPGAFHLPAEHLDARHHEIPRDREIVLYCS"
     gene            complement(12197..12367)
                     /locus_tag="AC16FU_02740"
     CDS             complement(12197..12367)
                     /locus_tag="AC16FU_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993323.1"
                     /translation="MFYILIDTIDVFLGSVGNPRLRQPSSPPARGRTCVTPRCLSKSA
                     TRALVASFGQEQ"
     gene            complement(12348..12764)
                     /locus_tag="AC16FU_02745"
     CDS             complement(12348..12764)
                     /locus_tag="AC16FU_02745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR2993324.1"
                     /translation="MEATIKQITGITFAGKATSNHWVMLDGKKEFGGAEGANSPMELV
                     LIALGGCTGMDVVSILEKMRVQFDRFEIALSSEQAKDYPRVFTRVKIEYHFWGTDIPK
                     DKVEKAIQLSQEKYCSVSAMVKKTARVTTEYVLHPH"
     gene            12909..14276
                     /gene="rlmD"
                     /locus_tag="AC16FU_02750"
     CDS             12909..14276
                     /gene="rlmD"
                     /locus_tag="AC16FU_02750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR2993325.1"
                     /translation="MQTQLRRGDTIELKVEDLAFGGLGVAHHNGMVVLVERGFPGDTV
                     LARIRRRKSSYLEAVAVELKAPSPERVAAVCQHHPICGGCKAQDLRYEVQIKHKTEQV
                     RQSLIHLGGFADPAVHPTILSPEVFFYRNKMEFSFFTDEAGKVCLGLHKSGTFDKMFD
                     LEKCWLQSELSNEIVAAVRDFAQRYELVPYHVRQHIGFLRFLAIRESKTNGQVLVNLV
                     TNEGEFPQAPDFVVTLTEKFPQITGIVRNISTSRANIATGEREELLWGKRYISEKISG
                     LEFSISANSFFQTNTKQAEMLFATTLAYADCTGSERALDLYSGTGTISLLLAQKVRHV
                     TGIEVVPEAVTMARLNAEHNGITNATFLSGEVRDLLPSQTRRGQFDLVIIDPPRAGLH
                     EDVVRHLIRLKVPNMVYVSCNPATLARDLKLFAAGGYKLIEVQPVDMFPHTPHIECVA
                     KLTAD"
     gene            14290..>14514
                     /locus_tag="AC16FU_02755"
     CDS             14290..>14514
                     /locus_tag="AC16FU_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993326.1"
                     /translation="MTERTSDEKNRVFAFTLIFVSLFVTTCAKQKNKESALRETVTQY
                     ITTWQERNIEKIYEKVYELELPEYKARVSFE"
BASE COUNT         3270 a         3789 c         3924 g         3531 t
ORIGIN      
        1 gtgtgcacac cggcgttttc tccctcggag aatccagtac ttgccactgt cgcagcgtgg
       61 acacctcagt cgtcttgcca tgccaccaac atatcaattt ggtggctgga atgggacttt
      121 acccttcctg ttttgtcgat gcaaccccat tttttgtcaa tttccacaaa agcaagctct
      181 tcagaaaaat tccaagcgaa atcgaacttt ggtttaatta tttcctttcc tgttttgtca
      241 ataaaagccc acttactatt aatttgcaca cgtgccaacc cttcagaaaa attgtcagca
      301 tcatgaaact tgggttttac aacatatctc cccagtttgt cgataaatcc ccatttccca
      361 tctattcgta cagctgctaa accttcagag aaactcatag cggtgtcgaa ttgcggaggg
      421 atgagcgttt ttccggtctt gtctatataa ccgcgtttcc aattgatctt tacaagtgct
      481 aatccttcgg aaaaagattc agctttgtcg aactgcgagg ggatgatcat ttttccagtc
      541 ctatcgataa agccccattc ttttccgtat attttctgaa ctggtgcaag gccttcagaa
      601 aatttccaag catcatcaaa ttgtggttca ataaccattt taccagtctt gtcgatatat
      661 ccccaatggc ccgtaaatac cattgctaaa ccttcagaaa aatcgtaggc actataaaat
      721 tggggtttga taaccatttt gccggtcttg tccatgtacc catatctgcc actacacgac
      781 caaaccaaag tcgcaatgct caccaagagt aatgtacttt tcctcattac catttcagtg
      841 aagctccttt gaccatcttt acccttttca agttggtatg gacgactcaa tatctattct
      901 atctcaaatt tatcattctg caggaacacc tggtactcgt ggtcggggag gagaggaaaa
      961 ggagcgctgg cgatctcttt ggagttcacc accggcaagt tcgaggaacg caggaaagag
     1021 gcgaattcct gggcctcttc gctcaatact aatttcgccg gtcgcgctcc ggctgaatcg
     1081 gcggtgtccc gtactaccac ttcggttaag cgcaggtatt ctttgagccc cagaaaggag
     1141 aattcctttc tccatttgat ggcgttggcg gttatgcggc agagctcgcc ggggcctttg
     1201 gaaagaagca ggcggtaccg gcccgatttg tacagctcct gaacctccag gtaggggtct
     1261 cccgatttgg ggattaccaa tttgaggctg gctaccggac ggtcgggctt gggcggatag
     1321 aagttttcgt gctcccccac tagccagacc aaaagttggc ccagtccgac taagagtgcg
     1381 gccaggagaa tgggcccgac gatgctcggc ccggatgact catcctggcg ggcggggggc
     1441 ttgcgagtcg aatccttcaa tttgcggaaa agcccccaga ggagtatcgc tacgcccagt
     1501 ccgataaaga gatatccaac cgtcgaaagc cgctcgagat tcataatttt tcacacgccg
     1561 aaagaacgca aaaaccgatc atttccgaca tgaaattgac ctgtagccga ggtgtcgcta
     1621 cgactctacc gtcaaacttc ggctgcgact ttgcacgggc cggtagaggc ccgctatatc
     1681 taactatcgg aagaagccga gaggtctgaa cgtttgggtg ggttggtggt cccctgcact
     1741 tcgtagtaaa agatcgattt tccggtcgag tccggcggtg tggtgtagcc gagcgtctgg
     1801 cctttattca ctttttgccc ccggctgacc ttcaattctc caaggcggcc gtagcgggtt
     1861 ttcactccct gtgcatggga aacgaccacc agattgccca ggctggagtc tctgccggaa
     1921 aactcgactg tcccttccgc cgtcgctcga acggaaactc gggcggtcgc ggccagaaac
     1981 gtcccgggca gaagcaggtc agatccagag agaagagagc gggggaggcg ggtgccactg
     2041 gccggcaagc cggttggcag tgcttctttt tgaggaggca aaactgacgc taccgatgaa
     2101 gaaatagttt cacttacaac cacttttgat gattcaggca tcgaaacatt ggtaggtgat
     2161 ttgctcgaat cttgtaacat tggtaattct attcctgcca ttttggcaag ttttactgtc
     2221 aaaacgcgat tatcaaacag ttctttttcc aacaaagtga ttttggatgt gtagtttctt
     2281 aatttttgat tttcaatttg taccgattcg agaagtgaag tcttggcagc caggcggctg
     2341 gaatagagaa ctgaaatgac caatccgatc acggtggcaa tgagcaatcc aatgatcaaa
     2401 taaaaaatga tgctgggaag gcggatggaa agtgcctttt ggtcgtcttc cgagaaaacc
     2461 gtaaaggtca tatggcgaat tttccgcatc aggacctcct ttccttatca aatcggctgt
     2521 tgggccggca tcctttgccg ctcagacggc gattttgagg agttgaagaa tccggttcag
     2581 gtcgtcgtcc gaatagaatt cgatgacgat tttgccgcgc tttttgcccc ggcgcaggat
     2641 cacgttcgtt ccgaagaagc gcttgagagc ctgttcaatc gtgtcgaaga gcgccgcggg
     2701 acgtttgagt ttcagccggc gccggatggg tcggggtgat tcaatcaagc gttccaattc
     2761 ccgtacggag attgatccct gcacgattcg ctctgcgagt tgattttgcc gctgggggtc
     2821 cgaaagccgc aagaggagcc gcgcctgtcc cggagagagc tcgccctccg cgatttttgt
     2881 ctgaattcct tgtggcagat tcagaagccg gagcgcattc gccacggagg aacgttcttt
     2941 acccactttg ccagctatgg cttcctgcga cagaccgaat tcggtcgcca gttgcttata
     3001 ggcccgggct tcctcaatgg ggtttaaatc ttcccgctgg aggttttcaa tcaacgccaa
     3061 ttccagcgcc atttctttcg acacattttc ccgcaccagt gccggaatag attcaaatcc
     3121 ggcaagctgt gcggcccgca cccggcgttc gccggcgact acctggtagt tttcgcctac
     3181 cggccggacg ataatcggtt gaagcactcc ctgatctttc agggaattgg ccagttcggc
     3241 caggcgttcc ggatcaaagg cctggcgcgg ctggtcgggg ttcggttgaa ttttcgccag
     3301 aagaattggc tggatacttt ctccttccat tcccgtttcg ccttccggaa tcagggcgct
     3361 caggcccttg cccaaagcga tcttttgcgt cattcgctgg tcgcctccgt agtttatttc
     3421 acagccgccg gttccaattc cgaaagaatt gtcctcgtca cgcccgcagg ttccaatgct
     3481 tgagggtccg cgccgtttcg ctccaggaat tctttcgcca gagcaatgta gttttccgcg
     3541 ccggtggaca aaatgtcgta cagaatgatc ggtttgccga aactgggcga ttcggaaagc
     3601 cggacgttgc ggctgatgat cgtctggtac accttgtcgg taaagaactt gcgcgcttca
     3661 tctgcaacct gcttggagag attcaaccgg ctgtcataca tcgtcaagag gacaccctca
     3721 attgccagac ccgggttcag gtgcttttga attagtcgaa tggtgttcaa gagctggccc
     3781 agaccttcca gcgcgtagta ttcgcattga atcggaatta aaaccgaatc agctgccgtc
     3841 agcgtgttaa cggtgagcaa gcccagcgag ggaggacaat cgacgaaaat gaaatcatat
     3901 gaattccgaa tgggggacac ggccgttgtc agccgctttt ccctgccgat tgcattgacc
     3961 agttctactt cggccccgac caaacgggta tgtgagggag ccacatccag ataagccagt
     4021 tcggatttga gaattatctc gcgtaacgga agttcctcta aaagagcttc ataaatcgtt
     4081 gttttgattt tacttttgtc taaccctatg ccggaagtag cattagcctg agggtcgata
     4141 tcgatcaaaa gagtattacg ctcggcggcg gccaagcaag ccgacagatt aattgcggtg
     4201 gtagtctttc caactccgcc tttttgattg acgacggcta ttactttacc caataaacat
     4261 ccccatttag gacaaactta agcaattagg gtagataaaa gcaagaactt ttttctttca
     4321 ccacggagac acggagggca cagagaaatg ctattccttc tttcttaatc taacttccga
     4381 aaaatgagaa agcaagtatt gagatttaat tcagggaccg gaacgttctc agcgctttcc
     4441 agccgcgcag tgccggtgaa gaattttagt tttttcaact cggacgggta attctttgtt
     4501 ttataggcca cggcaactcc gcctgagact aagagccgcc gggatagccg caccaccatt
     4561 ttcaaatcgg cgaaagcctt gtagagagta aagtcgaatt gttccgtgac cctgcggaca
     4621 tcagattgga taatttgaaa agacaatttt aggaccgtcc ggcactcctg aagaaagagg
     4681 gattttttgg gcttggcatc gatgagcgtg agttgcaagt caggtcgaac gatggccagg
     4741 ggaattcccg gaaaaccggc gccgctgccg acatcggcta tcttcgcatt ttggggaaaa
     4801 tcgattgccc gaagaaccga gagggaatcg acggtctgcc gggcgaggcc gaattgatta
     4861 gcctgggagg gggcgacgag gtcgaatttt ttccagtgtt tgaggaccag atcaaaatag
     4921 atgtaaaatt ggcttaattg aagattcgag ggttctaaat ttattagttt aagggcagaa
     4981 gggagatcga tttttgtttg ttctactttg ggtgaaggac aatgtttcac gtgaaacagt
     5041 ttaataactc ggtgatttac atattcttgc cgcgtgtcaa ctctcgagtt gtttcagatg
     5101 gaccatcaga acggcaatat ctcccggcgt aactcccgaa attcgtgagg cttgccccac
     5161 cgtagccggg cggatgcgct gcaacttttc ctgggcttca cgcttgaaac ctttcaaaga
     5221 taagtaatta atcctctccg gtatgcgcat tccttctgtt tttttgaact tggcgatttc
     5281 cttttcttgc ctgaggatgt aaccttcata tttgacttct aattcaactt gctgagcgat
     5341 ttgtggacga acgcagactg attccgagtc aatttctcga atatgccggt agtttacccg
     5401 gggatcgcgc aggaaggtga aaagagaaat tttctggtcg ggattcggag actttccgtt
     5461 atcgtttgtc cgcgcaatgt ccggtggccg gtcttcggcc tcaatgacca gcgggtcgtc
     5521 gtgctcgcct tctctctccg ccgttctatt tccattcatt aaaagcgaga acagctctcc
     5581 ccgccgatac cggctttttt gcagtcgatc cttctcccgc tcgatttctc cacggcgctc
     5641 ttggtatttc tgatagagcc gcttcggaac taaactcaat ttgtatccgt agggcatcag
     5701 ccggtccgcg gcgttgtctt cccggatagc gagtcggtat tcgacgcggg aagtaaacat
     5761 ccggtacggc tccagcggat ttttcgtcac caagtcgtca atcaaaacgc cgatgtaggc
     5821 ctctgagcgg tcgagcacga atggttcttc gcctttgatc tttaaagcgg cattgatgcc
     5881 ggccataatc ccctgggcgg cggcttcctc gtagccggaa gtgccgttaa tctgaccggc
     5941 aaaaaaaaga ttctcaataa gtttggtttc cagcgagtgc ttcagttgcg tcggcggaaa
     6001 aaaatcgtat tccactgcat aacccggttt gagcatttca acctgttcca gcccggggat
     6061 ggttttgagg gctgcaaatt gtacctcttc cggcagactg gtagaaaagc cgttgatgta
     6121 gactacgtcc gtctcatacc cctccggttc gaagaagatc tggtgccgtg gtttgtcggc
     6181 gaagcggaag attttgtcct ccacagaagg acaatagcgc gggccgatgc ccttaatttg
     6241 tcctgaatac atcggcgatt gatggatatt gtcacgaata atctgatgcg tctcagccga
     6301 agtgtaggtc aaccagcaaa aaagttgcgg atttttgatt ttgtcggtgc gcagcgaaaa
     6361 aggcacgggc ggatcgtcac ccggttgcag ctccgttttc gaatagtcga tactcttgtg
     6421 cgagaggcgc ggcggagtgc cagttttgag ccggccggtt tcaaacccca gtttctgcaa
     6481 attttcgctc aagtagaggg ccggcgattc tcccacccgt ccggccggga agttcttctt
     6541 tccgatgtgg attaagccat tcaaaaatgt cccggagcag aggaccacgg cttgtgcttt
     6601 atatctgttg ccggacgacc caacgacgcc ggtggccctg ccgcccgtgg tgagaatgtc
     6661 aatggcgcca tcttcgacga tctcaagccc cgcctgggac gtcaccacct ggcgcatgca
     6721 aagtttgtag agcaagcgat cgttctggga gcgcgaggac caaaccgcgg ggcccttgct
     6781 tttgttcaag attttgaact gcaccccggt ctgatcggcc acccagccca tctcaccgcc
     6841 caaagcgtcg atttccttaa ccaactgtcc cttggcctgg ccaccgatgg ccggattgca
     6901 cgacatttga gctatggtat ctcggttctg ggtaaaaagg gaggttctca gacccatccg
     6961 cgcgcaaacc aaagccgctt cgcagccggc atggccggca ccgactacaa tcacatcata
     7021 attttcttta taggttaaat ttgacatttt ccctttttca tttttgattt tttctcaaaa
     7081 taatacttca gcgatggctg tcaaatgccg gtccgggacg attctccata gactttttcg
     7141 gtttctgtac gtaatttgag aagtggagca gctttccctt aaaggagcac tttgcgaatg
     7201 gctgacggtt ctaaaaatct aaatgagatt tgcgacatca ccatcatcgg tgcagggccg
     7261 gtggggcttt tcgccgcgta ctacgccggg ctgcgggcgc tgcgggcgaa ggtgatcgac
     7321 agcctcccga aagtgggcgg gcagctcacc agtctctacc cgaagaagaa catttacgac
     7381 gtggccggtt tccccaaaat cttagccgag gatctggtac agaatcttct cgttcaggcc
     7441 ctgcagtacc gcccggaact ctgcctggag cagcgcattc agaaattaga atacgtcgat
     7501 ggcaatctga ttcaactttc ggatgaaaat ggcatccgac acctgacccg tacggtcatc
     7561 atctcggcgg gcaccgggtc gctcgtgccg cggcggctgg agttgccggg cgtcgaaaag
     7621 tatctcgatc acggcctcac ctatgtggtg ctcgatcccg aggagtatcg cggcaaaagg
     7681 gttctgattg tgggcggggg ggatacggcg ctggactggg cgctgacgct gaaagatatt
     7741 gccaaagaaa cgaccctcat tcaccgttcc ggaaaatttg cgggacatga agattcggtc
     7801 cgcaaactct tcgcttccca cgtccggacc cacatccata ctgaaataaa aacgattcac
     7861 ggcgatagtc gggttgccgg cgctaccatt actacccgcg agaaaaacga agagataacc
     7921 ctggatctgg acgccatcat cgtgtgcatc ggcttcacgc ccaacctggg ggcgattaaa
     7981 gagtggggtc tggctatcga gaaaaactcg attcaggtaa acagccggat ggagaccaac
     8041 ctgccggccg tctatgcggt gggcgacgtg gtagactacc ccggaaaagt taagttaata
     8101 gtcaccggct tcggcgatgc ggctgtggca gtttgccacg cggcgcaccg aatcaatccg
     8161 cagttgaaag tcttccccgg gtactccacg aattttgtgg agaaattgca ggccaaagtg
     8221 cagacctctg tagggggtta agccagtttg gaatcgaagt caagcaatct aatctgcagg
     8281 agaaaaggtt gattaagata cggaaggccg acgaacgtgg acacgcggac tacggttggc
     8341 tgagtaccta tcacactttc tcgttcgaca cttactacga cgccgcgtat atggggtttc
     8401 gctcgctgcg ggtcataaac gaagaccgcg tgcaaccagg gaatggattt ccgtcgcact
     8461 ttcaccggga tatggaaatc atttcctacg tgctggaagg tgccctggag cacagggata
     8521 gtttgggtac tggttccatt atccgaccgg gtgatgtcca gcggatgagc gccgggaccg
     8581 gcgtgacgca cagcgaatac aatccttcgc aaaccgacat tgttcacttc ttgcaaatct
     8641 ggattttgcc ggaacgaaaa gggttaaagc cgagctatga gcagaagcgg ttttcagacg
     8701 aagaaaagcg gggcactctg cgcctgatcg cctcccgcga gggccgcgat ggatcggtta
     8761 gcattcatca aaatgccagc gtctacgcca cttcacttga tatcggacaa gaacttgtcc
     8821 atcccctaaa gccgggccgg catgcctggg cacaggtggt gcgtggccgg gcaacattca
     8881 acgatcgcga gttaaaacag ggcgacggag cagctgcgag tgaggaaaaa gcattgaagc
     8941 ttgcaagccg tgaaggagcc gaagttcttg tttttgatct ggcgtagcaa agttatgagt
     9001 cggtaatacc aacagctcca cgtattctct tttgaaacgc ccatgaccca accgcaagaa
     9061 ccgcaaaaaa ccatcgactt ggccatctcc ggcatgacct gtgcctcgtg cgtttccacc
     9121 gttgaaaaag cgctgaagtc tgtgtcagga gtaacgcagg cctccgtcaa cttagccacc
     9181 gagcgagcac tggtcaccta cagaccggga gtcgccacgc tcgaacaaat gttcaaagca
     9241 atcgctgatt ccggatacaa ggccagggag ttatccgcag agacgcgcga cgagcgggaa
     9301 gaattactca aagaagagtt aacaactctg aagaagaaac tattcttcag tctgatagct
     9361 gccgctctcc tgatgctctt gggtttccag atgtacctgc cggggctgaa agcattcccg
     9421 cgttccctca ttccttggct cggactggtt ctggcgacac cggtccagtt ctgggccggg
     9481 gcgcgcttct atcggggttt ctgggcgcag ttgaagcacg gtcgcgccga tatgaacacg
     9541 ctcatcgcca tcggtacctc ggcggcgtac ttctatagcg ttgcggtgac attttcccct
     9601 tccggattca cctctgccgg gcaccgggcc gaggtttact acgaaaccgc tgcggctata
     9661 atcgctttaa ttcttttagg acggtatctc gagacccagg ccaaaggccg cacctcggcg
     9721 gccatcaaaa aactcctcgg acttgctccc aaaaccgccc gcgtgcggcg ggatggttcc
     9781 gagatggacc taccgatcga tcaggtattc gtcgatgata ccgtcattgt ccgccccggg
     9841 gaacggattc cggtagacgg acaagtgatc gagggatatt cagccatcga tgagtcaatg
     9901 gtgacgggcg agagtctgcc ggtggagaaa aaaatcgggg accgcgtcat cgggggaacg
     9961 attaataaga ctggcagttt cgtgtttcgg gccaccaaag ttggcaagga cacactcctg
    10021 gcgcagatta ttcacctggt ggaaaaggcg cagggctcca aagcgccgat tcagaagtta
    10081 gccgaccggg tggcggcagt gttcgttccc atcgtgctgg tcattgccgc aatcactttt
    10141 gcggtttggt ttcttttcgg gcccgagccc aaactgaccc gcgcattgct caactgcgtt
    10201 gcagttttga tcattgcctg cccctgcgcc atggggctgg ccaccccgac ggcgattatg
    10261 gtgggcaccg gccggggggc tgagatggga attctcatta aagatgccgg ggcgctggag
    10321 aaagcacaga aattagacac ggtggtgttc gacaaaaccg gaaccgttac cttaggcaaa
    10381 ccggcagtaa ccgccgtttt ccctttgaat gggtttgcgc cagagcaagt agtacgctgg
    10441 gccgcctcgg ctgaaaaacg gtccgagcat ccgctggcgg aggcggtgac ccgctatgcc
    10501 gaagagaaga aattcgcttt gagcgagcca accgccttca ccgccgagcc gggttttggt
    10561 ctggtggcga cggtggaggc gaaaagaatt ctggtaggga atgaagcgct cttgaggcgt
    10621 gaacggatct cacttgacgg agtctctgcc aaacaatttg ctgcgaattc aggtgccagt
    10681 ttcctctggg tggcggtgga tggtagggtg gccggcgctt tgacggtggc ggatacgttg
    10741 aaaccgaatg cggtctcagc cgtcgtccga ttgaagtctc tggggttgga gccggttttg
    10801 gcttcgggcg atcgggagga aacaaccgtt gaaatcgcca agaaattggg aatcgttgcc
    10861 tactatgccc agattcttcc gcaagagaag ggcgacttgg tgcgccttct ccaacagcgt
    10921 ggcaaaaaag tggccatggt gggcgatggg atcaacgacg cagtggccct ggctcaagcc
    10981 gacgtgggca ttgccatggg caccggtacc gacgtggcca tcgaaaccgc cgacattact
    11041 ctggtcaaag gtgatctctc cggcgtggcg cgggcgattg cccttgcacg taaaactgtg
    11101 aagacaatcc gctggaactt gttctgggct ttcatctaca acatcatcgg gattccgatt
    11161 gccgcggggg cgctctaccc tttcttcggc tggcagttga acccgatgct tgccgctgcg
    11221 gcgatggcgt tttcctcggt cttcgtggtg accaattcgc tccggctgag gaaatttcag
    11281 gagggtgtgt aatctcagca atttagtgtc ggggcgaatc tgtagatttg cccaaaatgt
    11341 gtcgggtgaa cctataggtt cacccctaca tttttaacaa accgcatggg aggaaagatc
    11401 gagtgaccga gacaattcaa tttctcattc gttacgggta catcgtgctc ttcatttcgg
    11461 tttttgccga gcagatcggg ttgcctttgc cggccattcc cctgcttttg gccgccggag
    11521 ctctggcggg tctgggtcgg ttgagtttcg ctttggtcgt cgttctggcg gtggccgggt
    11581 cgctggtggc tgatttcatc tggtacgaaa tcggccgccg ccgcggaggg cgggtgctga
    11641 acttcctctg ccggatttcg cttgaacccg actcctgcgt gcgccgcact gagaactttt
    11701 ttgcgcggta cggtgcacgg tcgctccttt tggccaagtt cattccggga ttcagcgtgg
    11761 tggcgccgcc attagccggg atcttcggca tgcggttggg gcggttcctc agtttcacag
    11821 cgcttggcgc tctcatctgg gccggaacat ttaccggcgt cgggtatttg ttcagcagcc
    11881 agttggagca ggtggctacc tatgcggggc agtttggggc gtcgctggtg gcggtgttag
    11941 tcggcgggct ggccctctat cttgcctgga agtacctcca aaggcggcac tttttggctc
    12001 agctgcgcgt ggcgcgcatt acgccggaag aattaaagca aaaactcgat gctggtgatg
    12061 aaatgattat cgtcgatttg cgccagtcct tagacttcga ggccgaaccc cacacgattc
    12121 ccggggcgtt ccacctgccc gccgaacatc tcgatgcgcg gcaccacgaa attccccgcg
    12181 accgcgaaat tgttctctac tgctcctgac cgaacgaagc aaccagcgcc cgggtggcgc
    12241 ttttgctcag acaacggggg gtgacgcagg tgcggcccct ggccggcggg ctggatggct
    12301 ggcgcaagcg gggatttcca acagaaccaa gaaaaacgtc aatggtgtca atgaggatgt
    12361 aaaacatact ccgtcgtcac ccgcgccgtc ttcttcacca tcgccgagac cgagcaatat
    12421 ttctcctgcg agagttgaat ggctttttcg actttatctt tcgggatgtc tgtgccccag
    12481 aagtggtact cgattttcac ccgggtgaag acgcgcgggt agtctttggc ctgctcggag
    12541 gaaagagcga tttcgaagcg gtcgaactgc acccgcattt tctccaagat gctcaccacg
    12601 tccatcccgg tacagccgcc gagagcgatc aggactaact ccattggact gttggccccc
    12661 tccgcgccgc cgaattcttt cttgccgtcc aacatcaccc agtgattgga tgtagctttg
    12721 ccggcgaagg tgatgcccgt gatttgcttg atggtggctt ccattaacac ctcacattca
    12781 gatcggctta gccggggatg aaatcctcgg ctgcgaactt tgcgggattt gtaaactatc
    12841 ccgcactgcg agagtataat cgaactcgct ctctttctcg tctgttctcc cgaaactata
    12901 tttcctgaat gcaaacgcaa ttgcggcgcg gagacacgat tgaactcaaa gtagaggatt
    12961 tggccttcgg cgggctgggc gtggcgcatc acaacgggat ggtggtactc gtcgagcgcg
    13021 ggtttcccgg tgatacagtg ctggcaagaa tccggcggcg gaaaagttcc tacctcgaag
    13081 cggtggcggt ggagctcaaa gccccgtcgc cggaacgcgt tgccgcggtc tgtcagcacc
    13141 atcccatctg cggcgggtgc aaggcgcaag acctgcggta cgaagtccag attaagcaca
    13201 aaaccgagca ggtgcggcag agtttgattc atttaggcgg gtttgccgac cccgccgtcc
    13261 atccaaccat actttcgccg gaggttttct tctaccgcaa caaaatggag ttctcgtttt
    13321 tcaccgatga agcgggaaaa gtttgcctgg gtctgcacaa atccgggaca ttcgataaaa
    13381 tgttcgattt ggaaaaatgc tggctccagt cggaactctc caacgaaatc gtggctgccg
    13441 tccgcgactt tgcacaaagg tacgaacttg tcccctacca cgtacgccag cacatcggct
    13501 tcttgcgttt tctcgcgatt cgagaatcca aaacaaatgg tcaggtgtta gtaaacttgg
    13561 tgactaatga aggcgagttc ccgcaggcac cggattttgt ggtgactctc accgaaaaat
    13621 tcccacaaat aaccggcatc gtacgcaaca tatccacttc ccgggcgaac atcgccaccg
    13681 gcgagcggga ggaactcctc tggggcaagc ggtatatttc cgagaaaatt tccgggctgg
    13741 agttctccat ctcggccaat tctttttttc agaccaatac caagcaggcc gaaatgcttt
    13801 tcgccacgac gctggcgtac gccgattgta ccggcagtga acgcgccctc gacctctaca
    13861 gtggcaccgg aacgatttcg ctgctcttgg cgcaaaaggt acggcacgtt accggcattg
    13921 aggtggttcc ggaagcggtg accatggccc ggctgaacgc ggaacacaat gggattacga
    13981 acgcaacctt tctctccggc gaagtgcgcg atcttttgcc atcacaaacc cgtcggggac
    14041 agttcgactt ggtgattatc gacccgcccc gcgccggcct gcacgaagat gtcgtccgcc
    14101 acctcattcg cctgaaagtc cccaatatgg tttacgtctc ctgcaatccg gcaaccttgg
    14161 cgcgcgacct gaagctcttt gccgccggtg gttacaagtt gattgaggtt caaccggtag
    14221 atatgtttcc gcacacgccg cacatcgaat gcgtggctaa actgacagcg gattaggaag
    14281 ttcaaacaga tgacagagcg aacttcagat gaaaaaaata gggtttttgc ctttacgctt
    14341 attttcgttt cccttttcgt tactacctgc gctaaacaaa aaaataaaga atccgctttg
    14401 cgggagaccg ttacacaata cataaccacc tggcaagaga ggaacataga aaagatttat
    14461 gaaaaggtat atgagttaga actccccgaa tataaagctc gggtttcatt cgag
//
LOCUS       DBNGZA010000125         7762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_871797, whole genome shotgun sequence.
ACCESSION   DBNGZA010000125 DBNGZA010000000
VERSION     DBNGZA010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_871797"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16FU_02760"
     CDS             complement(<1..308)
                     /locus_tag="AC16FU_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR2993327.1"
                     /translation="MAKPTIIAGNWKMHKTDAEARALALALRKFIPSGSAQVVLCPPF
                     IALAGVAQAISGSALHLGAQNLYPAKEGAFTGEISGAMLIALGCTYVIIGHSERRHIL
                     G"
     gene            complement(1353..2546)
                     /locus_tag="AC16FU_02765"
     CDS             complement(1353..2546)
                     /locus_tag="AC16FU_02765"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR2993328.1"
                     /translation="MAKLSIDDLKLRGKRVLVRVDFNVPLENGRVADDIRIVASLPTI
                     KKILADGGRAILCSHLGRPKGKPTPEFSLLPVARRLEELLGKKVAFATDCVGPVAEQA
                     VANLRDSECLLLENLRFHSEEEKNDPAFSAQLAKLGDLYVNDAFGSAHRAHASTEGVA
                     RLFQQRAAGYLMQKELHYLGKATANPKRPFIAILGGAKVSDKIAVIENLLSKVDALLI
                     GGGMAFTFLKAQGKEIGGSLVETEKIETAKMLLQKAKEKKVDLVLPVDCVVAEKIEVN
                     VPTAVVPVDRILPNQKGLDIGSRTVELFANKLSTAETVIWNGPLGVFEINAFAQGTFA
                     IAKTLAQLTQKGTVTIIGGGDSAAAVAKAGLANLMSHISTGGGASLEFLEGKELPGVV
                     VLTDK"
     gene            complement(2583..3275)
                     /locus_tag="AC16FU_02770"
     CDS             complement(2583..3275)
                     /locus_tag="AC16FU_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993329.1"
                     /translation="MQPLRFLFGKTHFILDFSIPLALLLHAIPAAAEENREKPRFNQL
                     SGRHPVVSVPMVQTIGPLRFSIARLEAARLAYPNFGNQKRDQHFFVSNSDGEHGERPQ
                     PSIAQPIGIGLFFAAIGFFAGAGIGAGILGGSDDGEFSRFEGAIIGAPIGEATFMPVG
                     VHLGNNRQGFFPIVFLNSLVVGGVGIWAASATDKDIHPAITLSATVIAQLLTCVSIER
                     AGSRHRRSNLRE"
     gene            complement(3305..4351)
                     /gene="gap"
                     /locus_tag="AC16FU_02775"
     CDS             complement(3305..4351)
                     /gene="gap"
                     /locus_tag="AC16FU_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR2993330.1"
                     /translation="MSIRVAINGFGRIGRLVFRAGYRNPDLEFVAVNDITDAPTLAHL
                     LKYDSVHRRMEADVRAEGSAIVVNGHRTEVLAEKEPANLPWKKLGIDVVVESTGKFTD
                     REAAVKHISAGAKKVLISAPAKKPDVMIVLGVNHRIYDPTKHQIVSIGSCTTNCLAPV
                     AKVLHDRFGIKRGFMNTVHAYTNDQRILDQPHKDLRRARAAAYSIIPTTTGAAKAIGQ
                     VIPELEGKLDGVAVRVPISDGSLLDLTCELEKDVTAASVNQAMKEAAEGGMKGILQYC
                     EDPIVSADVVGNPYSAVFDAQATMVIGKNLVKVFAWYDNEWAFSVRMVETIQIMMAGA
                     VGDKTERIATVAAR"
     gene            complement(4376..5032)
                     /locus_tag="AC16FU_02780"
     CDS             complement(4376..5032)
                     /locus_tag="AC16FU_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993331.1"
                     /translation="MGVIFKKILPGLILLSSVLTGTVAPKEFSQAHQFGGRFGILRIK
                     DAALGLNFPGFEIQKDGTLPYIEFFYQPRWNRYLSLDLSFGAVGRGEVRYIDQVNRLY
                     FFSSVNVYPISVGVNLQPLAFGHQPRFFPVMRGGGTFAIATESDLFGVGSKTQSAFGV
                     YLAPGLDVALGSFFFTAEAGYRTARFRDPVGGIKDYSGVFIGLGVGYSYSGRVPGKHH
                     "
     gene            complement(5160..5879)
                     /locus_tag="AC16FU_02785"
     CDS             complement(5160..5879)
                     /locus_tag="AC16FU_02785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2993332.1"
                     /translation="MPIQAVTFDLWETLVAHTPENDVAIRQLRYERMQTALGREIHRE
                     QFQTAYRRSWEKFEEIWITNREVQTEEQLDIFLTLLFNSSRPALNAETRKKLEDAYLS
                     PVRDHPPVVYPDVLPVLTELQKRKLPLGMICNTGRTPGRLLRPILQAQGILDFFRVAL
                     FSNELDLRKPAPEIFHVAIRELGAVPETTVHVGDRPNLDIQGALGAGLQAVQIDRYEL
                     YQPTGKWPFIRSLPELIPLLD"
     gene            complement(5888..>7762)
                     /locus_tag="AC16FU_02790"
     CDS             complement(5888..>7762)
                     /locus_tag="AC16FU_02790"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2993333.1"
                     /translation="LTLLTYLPAFLGFGLMALALSVAKGWLTIEPVIGFLGKIRPLFQ
                     PGNLEPANWGIYLLWFVLEFAVRVKKKEVVINARQRQWLQWGVVLPLLVYVVFNQVLP
                     LFWSWGLLKWSALVILPSQAVLAYLILKERLLEIGVVIKRSAIYAILSASLIGIFVLL
                     VLGISQLMVLLTGQTSILAAFAAALLTAIVLNLYRQKFQNYLDRKFFKDRYNYQRALL
                     EFSKELGRLENLETLLAKISKQFAEALHLSNCLPFLYNQKADAYVMVAPYGLAEPVLS
                     QVRYAASQFGLATLLVQEKHPLDFYDLETKPLYFYLPVVEKVALKKTDTALAVPLLVK
                     EKLVGMLLLGNKKSGDLFSSEDVDFFATLSAPLAVAVENARLHQEELGKKELEREIVL
                     AGEVQKRFLKTVPAQLPGLEIGALNLPSRQVSGDLYAIIPLDEKRLGIAIGDVAGKGM
                     SASLLMALVQATLETLAPSVAAPAELLQRLNTAVCRNSTPGRFVTLFYGIYDTETKRF
                     AYANAGHNPPVWLGRQGVRALDEAGLLLGVDPKARYPQGLVHLKPEDLVVLYTDGVTE
                     ASNTDEHRFEEERLHQCLLKSRTLSAPDVLQAVVEAVRAFAGTSEFEDDLTLVGLKVV
                     "
BASE COUNT         1742 a         2233 c         2081 g         1706 t
ORIGIN      
        1 cccagaatat gccgccgctc ggagtggccg attatgacgt acgtgcaacc gagcgcgatg
       61 agcatcgctc cggatatttc gccggtgaac gccccctctt tggccggata gagattctgc
      121 gcacccaggt gcaaggccga accagaaatt gcttgagcga cgccggctaa ggcgataaag
      181 ggtggacaga ggactacttg tgctgatcca gatggaataa attttcgcag cgccaaggcc
      241 agcgcccgcg cttcggcatc ggttttgtgc atcttccagt tgccggcgat gatggtgggt
      301 ttagccacga gagggtactg atgtttggct atctgataag tttgctactc gactgcgaat
      361 ttttggtacg gcacaaaggc tgaggctcgg gctgaatact ttaccgcaac ttcatttcaa
      421 tcgggcgtga taccgcagtt gttgcacaca acctgctggg aacagacgcg accattacag
      481 gacgagtagc aagttatgtg ctcacaccag taaatacatt ctccgttcgc ccatcccttg
      541 tcacactgac aaacgctgag acagttgttg cagtagcagg gtcctgccgc cgctggcacc
      601 ttgggtgcca ttacaattcc tacggtaaat gaaatgagga gaactgcgac gaacgcaacc
      661 aaaacttttc tcatggcact ctccttccgt ggtttgtacc gagttcagcc cttgtgccgt
      721 cgtaccggtg tgttcataac tgaatttccc ggtcttgtca aatgaattcc atggttttaa
      781 atgagaataa gaggtgaagt tttatttttt tcgtcagtac cacatccccc gtagttcttt
      841 tccttacaga aactccagcg ccgccccgtc gctcatggag actatgtgtg ctaaagtcca
      901 aaaacttggt acggcacaaa ggctgaagaa tcggtcttcc ttacacttct atcctaccgg
      961 caaaccggtc tgcaaaagct agtgcacgaa cccgagccca ggatacagcg agccccgctg
     1021 ttgctacaag ttccgtactg gacgggttta cggagcccag gagtactgca atccacacag
     1081 gtggtacagt cgtcacttat acaggtgccg ccgatgccac attggggcgc tgcaatggac
     1141 aacgcaggca ccagaaccaa tcctaccgca aaagagacca gcaggactac ggctaagaca
     1201 atgattggct ttttcatgac atcctccttc cctggtttta accgacctca gcctttgtgc
     1261 ttctgtaccg gctattttca tagccgatat tgtcatcctt gtcaagtact tttcctgacc
     1321 cgcgggaatc ctcagacgag aaagcccaaa atctatttgt ccgtcagaac caccactccc
     1381 ggcagttctt tcccctccaa aaattccagc gatgccccgc cgccggtgga gatgtggctc
     1441 atcaggtttg ccaatccggc tttggccact gctgccgcag aatctccgcc gccgatgatg
     1501 gtaaccgtcc ctttttgcgt cagctgagcg agcgttttgg caatggcaaa agttccctgc
     1561 gcaaaggcgt tgatttcaaa cacgcccaga ggcccgttcc agataaccgt ctcagcggtg
     1621 ctcaatttgt ttgcgaacag ttcaacagtg cgggagccga tgtctaatcc cttttggttt
     1681 ggtagaattc ggtcaaccgg gacgactgcg gtgggaacat tcacttcaat tttttccgcc
     1741 accacgcaat ccaccggcag gacgagatcg acctttttct ctttcgcttt ttggagtaac
     1801 attttggctg tctcgatttt ttccgtttcc accagcgagc cgccgatctc cttcccctgc
     1861 gctttgagga acgtgaaggc catcccgccg ccaataagca gggcgtcaac tttcgaaagc
     1921 agattctcaa tgacggcgat cttatcagat accttcgctc caccgagaat ggcaatgaag
     1981 ggccgcttgg gattggccgt cgctttccct aaatagtgca gttccttctg catcaggtag
     2041 cccgccgctc tttgctggaa aagtcgcgcg actccttcag tagaagcatg cgcccggtga
     2101 gccgagccga aggcgtcgtt gacgtagagg tctcctaatt tcgccaactg ggccgagaaa
     2161 gcgggatcat ttttttcttc ttccgagtga aaccgcagat tttctaagag caaacattcg
     2221 ctgtcccgaa gatttgcgac tgcttgttct gcgaccggcc cgacacagtc cgtcgcaaag
     2281 gcaacttttt tgcctaagag ttcttccagt cttcgcgcca ccggcaaaag gctgaattct
     2341 ggcgtcggct tccctttggg acggcccaga tgagagcata aaatcgcccg gccaccgtcg
     2401 gcgagaattt ttttgatggt aggtagcgag gcgacgatac gtatgtcgtc ggcaacgcgc
     2461 ccgttttcca gcggcacgtt gaaatcgacc cgcaccaaaa cgcgcttgcc gcgtaacttc
     2521 aggtcgtcaa tcgagagctt ggccatgtta tcaggttggt tcaccccgct attaaatatg
     2581 gtctattccc ttaagttact acgtcggtgt cgacttcctg ccctctcaat cgaaacacac
     2641 gtaagtagtt gcgcaatgac tgttgcactc aaggtaatcg ccggatgaat gtctttatcc
     2701 gtcgccgacg cggcccagat gcccactccc cccacgacca gtgaattcag gaagacaatc
     2761 gggaaaaacc cctgccggtt attgccgaga tgcacgccca cgggcataaa agtcgcttct
     2821 ccgatgggag ctccgataat agcaccttca aatctgctaa actctccgtc atccgatcca
     2881 ccgagtattc cggctccaat acccgccccg gcgaagaagc cgatcgccgc aaagaaaagg
     2941 ccgataccga ttggctgggc tatggagggc tgtggccgtt cgccgtgctc accatccgaa
     3001 ttggatacaa aaaaatgctg gtcgcgtttt tgattaccaa aattcggata agcgagtctc
     3061 gccgcttcga gtcgtgcaat tgaaaatcgc agcgggccta ttgtttgaac cattggaact
     3121 gacacaaccg ggtgccgacc gctgagctga ttgaagcgcg gtttctccct attttcctct
     3181 gctgcggctg ggatggcgtg caacaacaga gcaaggggga tactgaaatc aagaataaag
     3241 tgcgtttttc caaataagaa ccgcaggggc tgcatgattc gacacctttc tcaatggtgt
     3301 cactctaacg ggccgccacc gtcgcaatcc gctcggtctt atccccgacg gcaccagcca
     3361 tcattatctg aattgtctcg accatccgca cagaaaaggc ccactcgttg tcgtaccagg
     3421 cgaaaacttt taccaggttt ttacctatca ccatggtcgc ctgcgcgtca aagactgcgg
     3481 agtaggggtt gcccaccaca tcggcggaga cgatcgggtc ctcgcaatac tgcaggatgc
     3541 ctttcatccc gccttcggcg gcctctttca ttgcctggtt cacggaggcg gcagttacat
     3601 ctttttcgag ttcgcaggtc aagtcgagga gcgagccgtc ggaaatcggc acccgcaccg
     3661 cgacgccatc caacttcccc tctaattcag gaataacctg cccgatcgcc ttggccgcgc
     3721 cggtggtggt cgggatgatg gaataagccg ccgcccgcgc ccgccttaag tctttgtgcg
     3781 gctggtcgag gatccgctgg tcattggtgt aagcgtgaac cgtgttcata aaaccccgct
     3841 tgatgccgaa gcggtcgtgg agcactttgg ccaccggcgc caggcagttg gtcgtgcaag
     3901 agccgatgga gacgatctgg tgtttggtcg gatcataaat ccggtggttg acgcccaaga
     3961 cgatcatcac gtccggcttt ttggcggggg cggaaatcaa aactttcttc gctcccgctg
     4021 aaatatgttt caccgccgcc tcccgatcgg tgaacttgcc agtcgattcc accaccacgt
     4081 caattcccaa ctttttccag ggcaaattag ccggttcttt ttcagccagt acttcggtgc
     4141 ggtggccgtt gacgacaatc gcactgccct cggcgcgcac gtcggcttcc atgcgccgat
     4201 gcaccgaatc gtacttcaaa aggtgcgcta aggtcggcgc gtcggtgatg tcgttgaccg
     4261 cgacaaactc caggtcggga ttgcggtatc cggcgcgaaa gactaaacgc ccgatccgcc
     4321 cgaacccgtt aatggctact cgaatcgaca tgtctcctcc acaattttcg atgcttcagt
     4381 ggtgcttccc tggtacgcga cctgagtacg aataccctac gcccagtccg atgaaaaccc
     4441 cgctgtaatc cttgatcccg ccgaccgggt cacgaaatct ggccgtccgg tagccggctt
     4501 cagccgtaaa gaaaaatgag cccagcgcca catccaaacc gggtgccaag tagacgccga
     4561 aggccgattg ggtcttcgac cctacgccaa aaagatcgct ctcggtggcg atggcgaagg
     4621 tcccgccccc gcgcatgacc ggaaagaagc ggggctggtg cccgaacgcg agcggctgaa
     4681 ggtttacccc gactgaaatc gggtagacat tgacggaact aaaaaaatag agccgattga
     4741 cttgatcaat gtagcggact tcacctcggc caacggcgcc gaaagacaga tccaacgaga
     4801 ggtagcggtt ccagcgcggc tggtagaaga attcgatata cggcagggtg ccgtctttct
     4861 gaatttcaaa ccccggaaaa ttgagcccca acgccgcgtc cttgatccgc aggatgccga
     4921 accgcccgcc gaactggtgc gcctgcgaga attctttcgg cgcaaccgtc cccgtgagaa
     4981 cgctgctgag caggatcaac ccgggtaaga tttttttgaa gattactccc atacgaaact
     5041 ccgactgttc acccttcaag gatacgacac cgatgctaat ttctctatgc cggaaagtca
     5101 agacgacggt cgaaaggaaa ttcgagaatt cataaattgc ttctctcttc aagggggaat
     5161 tagtccagaa gcgggatcag ttccggcaaa ctgcggatga agggccattt gcccgtaggt
     5221 tgatagagtt cgtagcggtc aatctggacg gcctgaagtc cggcacccaa agcaccctgg
     5281 atgtcgagat ttggccggtc gcccacgtgc acggtcgttt ccggcacagc accgagttcc
     5341 ctgattgcaa cgtggaagat ctccggagcc ggcttgcgca ggtcgagttc gttggaaaaa
     5401 agagccacgc gaaagaagtc gaggataccc tgcgcttgca aaatcggccg caatagccgc
     5461 cccggcgtcc ggccggtatt gcagatcatc cccaggggca acttccgctt ttgcagttcc
     5521 gttagaaccg gcagcacgtc ggggtagacc accggcgggt ggtcgcggac cggagacagg
     5581 taggcatctt cgagtttctt tcgagtttca gcattcagcg cggggcgaga actgttaaag
     5641 agcagtgtca ggaaaatgtc cagttgctcc tcggtttgca cttcccgatt ggtgatccag
     5701 atttcctcaa atttttccca cgagcggcgg taggccgttt gaaactgttc ccggtgaatc
     5761 tcccggccca gtgccgtctg catccgctcg tagcgcaact ggcggatggc cacgtcgttc
     5821 tccggggtgt gggcaaccag cgtttcccag agatcaaagg tgacggcttg aatcggcatg
     5881 gtgaaagtca caccaccttg aggccgacca aggtcaggtc atcctcaaat tcgctggtcc
     5941 cggcaaaggc acgtaccgct tccaccaccg cttgcagcac atcaggggcg gagagcgtcc
     6001 ggcttttgag caggcactgg tgcagccgtt cttcttcgaa ccggtgctcg tcggtattgc
     6061 tcgcttcggt aacgccatcg gtgtagagca ccaccaggtc ttctggcttg aggtgcacca
     6121 gcccttgcgg gtagcgggcc ttgggatcga cccccaggag caaaccggct tcatccagcg
     6181 cccgaacgcc ctgccggccc agccagaccg gcggattgtg tcccgcattg gcgtaggcga
     6241 agcgtttggt ctccgtatcg taaatgccgt agaagagagt cacgaagcgg cccggagtgc
     6301 tgttgcggca gacggcggtg ttcaagcgct gcaaaagctc cgcgggcgca gcgaccgaag
     6361 gcgccaaagt ctccagagtc gcctgcacca gggccatcaa gagcgaagcg ctcatccctt
     6421 tgccggccac gtcccctatg gctatgccca gccgcttctc atcgagcgga atgatggcgt
     6481 agagatcgcc ggacacttgc cgggaaggca aattcaaagc gccgatttcc agccccggta
     6541 gctgcgcagg gacagtttta aggaaccgct tctgcacctc tccggctaag acgatctcac
     6601 gttccagttc tttttttccc agttcttctt gatgcaagcg ggcgttttcc accgccaccg
     6661 ccagaggcgc cgagagggtc gcaaagaaat cgacgtcctc agagctgaac aagtcgcccg
     6721 actttttgtt ccccaggagc agcatcccga ccagtttctc tttgaccagg agcggcaccg
     6781 ccagtgcggt gtcggtcttt ttcaaagcca ctttttccac caccggcagg taaaagtaga
     6841 gtggcttggt ctccagatca taaaaatcca gcgggtgttt ttcctgcacc agaagagtcg
     6901 ccagcccgaa ctgggaggcc gcgtagcgca cctgggagag caccggctcg gccaggccgt
     6961 acggcgccac catgacgtag gcgtcggcct tctggttgta gaggaaaggc agacagttgg
     7021 acaaatgcaa tgcctcggca aactgtttgg aaattttggc cagcagcgtt tccaagttct
     7081 ccaggcgacc cagctctttg gagaattcca gcaaggcccg ctggtagttg taccggtctt
     7141 tgaagaactt gcgatccagg taattctgaa acttctgccg gtagagattc aggacgatgg
     7201 cggttaaaag ggcggcggcg aaagcggcca aaatcgaggt ctggccggtc aagagcacca
     7261 tgagctgact gatgccgagc accaacagaa cgaagatacc gatgagactg gcggaaagaa
     7321 tggcgtagat ggcggagcgc ttgatcacca ctccgatttc caagagccgc tctttcagga
     7381 tgagatacgc cagcaccgcc tgcgagggga ggataaccag agcgctccat ttcaaaagtc
     7441 cccacgacca gaagagcggc agtacctggt tgaagacaac gtacacgagc aacgggagaa
     7501 ccacacccca ctgcagccac tgcctttgcc gggcattgat cactacttct ttctttttta
     7561 cccggacggc gaattccagc acaaaccaga gcaaatagat accccagttc gccggctcca
     7621 ggttaccggg ttgaaacagc ggtcttattt ttcccaaaaa gccgatgacc ggctcaatcg
     7681 tcagccaccc tttggcgacg gagagtgcca gtgccatcag gccgaatccc agaaaagcgg
     7741 gcaggtaggt caaaagggta ag
//
LOCUS       DBNGZA010000126         4283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2250660, whole genome shotgun sequence.
ACCESSION   DBNGZA010000126 DBNGZA010000000
VERSION     DBNGZA010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2250660"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="hslV"
                     /locus_tag="AC16FU_02795"
     CDS             <1..479
                     /gene="hslV"
                     /locus_tag="AC16FU_02795"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR2993334.1"
                     /translation="LGGDGQVTFEDTILKHNAKKLRRLHNGTILAGFAGASADAFALF
                     DRFESKIEEYKGNLPRAAVELAKDWRTDRYLRRLEALLAVLDKEHALIISGNGDVIEP
                     DDKIVCIGSGGAYALAAARALIQHAKLDAKTIVKRSLEIASEICVFTNDKISIEEL"
     gene            515..946
                     /locus_tag="AC16FU_02800"
     CDS             515..946
                     /locus_tag="AC16FU_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR2993335.1"
                     /translation="MERYRHTQIGYVTIGATAPVFIFFVYVMTFIPFNGYVVAGFLTL
                     GLVSGLFASLTVLIDGEHLKIRFGPGVIRKKFPLREITVCRVVKNPWYYGWGIHLTPH
                     GWLYNVSGFSAVEIAMRSGKKYRIGTDVPQELERAIKQAIS"
     gene            1043..1834
                     /locus_tag="AC16FU_02805"
     CDS             1043..1834
                     /locus_tag="AC16FU_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993336.1"
                     /translation="MKTSIHSRRWCKFFFLFAVISFSIPKVYAQRDTTQIKEPQNSLR
                     SGAWALLFEVGSNFTLEAFQGFTISAKRHFSDKRALRLGLGISGSVSLDDEVIDRQLK
                     GDTVTFQEDQEGDLNNESVDLIVQHLWYANPKAEVNFFFGVGPLLRFSHSKSETDATE
                     ISGGITTLINSTSKETRWTAGFSAVLGVEWFATKHISFLAEYVNSLEHEWFKRTTNYK
                     VTTAGQTTEDRTEVTRRTLRFNPEHRGGVPLVSFGSVKFGLSVYF"
     gene            1872..2489
                     /locus_tag="AC16FU_02810"
     CDS             1872..2489
                     /locus_tag="AC16FU_02810"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR2993337.1"
                     /translation="MKAVAGTTVNDLIEELTAICSNAKGDAQVPKVKDCLSRFLEGNL
                     ELPPQYLQPAKDHYARHLLYRQPNGGFTIVVMVWSPGQATPVHDHAGTWCVEGVYQGS
                     MKVERFHLRPLANDGERSRTTDQNIVQMTAGEILFPGKGQTGALIPPVEHHRMGNESR
                     NVAVTIHVYGGELSKCKVFEPLGNERYLAKDKTLTYTTERPVVVA"
     gene            2714..3352
                     /locus_tag="AC16FU_02815"
     CDS             2714..3352
                     /locus_tag="AC16FU_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="resistance to Congo red protein"
                     /protein_id="HIR2993338.1"
                     /translation="MRERYEEAKKDLEKNSFVKRVEFREYSEGGMHHFSLDVTVRQFQ
                     NLNEIQKKIPPYIEDSGEESDFRIEKLPSGNISFRQKFGESPAESTEAEADTSERNPW
                     LKFGEFMAKASISTAFGNRYFTVRLHAPKIVSTNGNLDDRMQTAEWKLALVDLLSENP
                     YEQELKAEMHPPGKGLLWIVAAILLVAVIGGITLVTMRRRKRGVEPMAPAGN"
     gene            3416..3823
                     /locus_tag="AC16FU_02820"
     CDS             3416..3823
                     /locus_tag="AC16FU_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2993339.1"
                     /translation="MFTTGLSEIVLVVKDVAASARFYREVVGLTLNEAAASDKNWAWF
                     WAGVPGHPQSVAVTQDKDLLFAEKSPLPEGQRWGQVHYAFNVPREKLAEAVEHVRGKG
                     VEVYGPKHFKWMKATAYYFYDPDGNLLEFWSPD"
     gene            3946..>4283
                     /locus_tag="AC16FU_02825"
     CDS             3946..>4283
                     /locus_tag="AC16FU_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993340.1"
                     /translation="MNMFSRKRIVKIGIVSIPGKRREKNRLKYVSAHYEQAFFSVRDS
                     LSGKYPVEQINLKKLAKFERLFKRGIQILVLPEAGVITHQQLNNLEAFVKLGGIVFAD
                     YPSLAFDALDT"
BASE COUNT         1065 a         1035 c         1208 g          975 t
ORIGIN      
        1 cgctcggcgg cgacggccag gtgacctttg aggacaccat cctcaagcac aacgccaaaa
       61 aactccgccg cctgcacaac gggacgatcc tggcgggctt tgccggggcg tccgccgatg
      121 cctttgcgct tttcgaccgg ttcgagagca aaatcgaaga gtataaaggc aacctgcctc
      181 gcgcggcggt cgaattagcc aaagactggc gcaccgaccg ctacctgcgg cggctggaag
      241 cgcttttggc cgttttggac aaagagcacg cgctcatcat ctccggcaac ggcgacgtga
      301 ttgagccgga cgacaagatc gtctgcatcg gctccggcgg ggcgtatgct ttggccgccg
      361 cgcgggcgtt gatccagcac gccaaattgg atgccaaaac catcgtcaaa cggtcgctgg
      421 agattgcgtc ggagatttgc gtattcacga atgataagat ttcgattgag gagttgtaag
      481 ggtttaacgt ccgcacccgc ccggcaataa tcgcgtggag cgctaccggc acacgcagat
      541 cggctacgta accatcgggg ccaccgcccc ggtgttcatc ttctttgtgt acgtgatgac
      601 ctttatcccc ttcaacgggt acgtcgtggc ggggttcttg accctgggac tcgtctcggg
      661 gctctttgcc tcgctgacgg tcttaatcga cggagagcac cttaagattc gctttgggcc
      721 gggggtgatc cggaagaaat tcccgctcag agaaattacc gtctgccggg tggtgaagaa
      781 tccctggtac tacggctggg gcatccacct gaccccgcac gggtggctct acaacgtttc
      841 cggcttctcg gcggtggaga ttgccatgcg gtccgggaaa aaataccgca tcgggacgga
      901 cgtgccgcag gagttggagc gggcgatcaa gcaagcgatt tcttaaaagc gtacagggat
      961 gttatattgt taaggctaca ggcctcatcg gctagtgtgt tatgatttgt gctgagggaa
     1021 gtcgaaggaa aggggcacca ccatgaaaac gtcaattcat tcccggcgat ggtgcaaatt
     1081 ctttttcctt tttgcagtga tttctttctc tatcccaaaa gtttatgcac aaagggatac
     1141 gactcaaatc aaagaacccc aaaacagtct tcgatccggc gcgtgggcgc tcctgtttga
     1201 agtcgggagc aattttaccc ttgaggcctt tcaggggttt accatttcgg ccaagcgtca
     1261 tttttccgac aaaagggctc tgcggctcgg cctaggcatc agtggctcgg tctcgctgga
     1321 tgacgaggtg atcgacagac agctgaaagg cgacaccgtc acatttcagg aggatcagga
     1381 gggcgactta aataatgaaa gtgtagacct cattgtacag catctctggt atgcaaatcc
     1441 caaagccgaa gtgaactttt tctttggcgt aggaccgctc ctgcgttttt cccacagcaa
     1501 atctgaaact gatgcaacgg aaatttcagg tggaatcacc accctgatca actctacctc
     1561 caaggagacg cggtggacgg ctggcttctc cgcagttttg ggagtggagt ggtttgccac
     1621 aaagcacatc agttttttag cggagtacgt gaactctctg gaacatgaat ggttcaaacg
     1681 aaccacgaac tataaagtca ctaccgccgg tcagacgacg gaggaccgca ctgaagttac
     1741 ccggaggacc ctgcgcttca atcccgagca caggggcggg gttccactgg tgtcatttgg
     1801 ctccgtcaaa ttcggcttgt cggtttactt ttagcgcgtt atgatttccg ccagaggaag
     1861 ggagggcctg aatgaaagcc gtagctggaa caaccgttaa cgacctgatc gaagaactca
     1921 ctgccatctg ttctaatgcc aaaggcgacg cgcaggtgcc caaagtcaaa gattgcttga
     1981 gtcgcttctt agagggcaac ctcgaactgc cgccgcagta cctccagccc gccaaagacc
     2041 actacgcacg ccacctttta tacaggcagc cgaacggtgg atttacgatt gtggtgatgg
     2101 tttggagccc ggggcaggcc acaccggtgc acgaccacgc cggcacctgg tgcgtggagg
     2161 gggtgtacca ggggagtatg aaggtggagc gttttcattt gcggcctctc gcaaatgatg
     2221 gtgagcggag tcgaaccaca gaccaaaaca tagttcagat gaccgccgga gaaatccttt
     2281 ttcccggcaa ggggcagacc ggcgcgctca ttccgccggt ggagcaccac agaatgggaa
     2341 atgaaagcag aaacgtagcc gtgaccattc acgtgtacgg cggggaactc tccaagtgca
     2401 aagtctttga gccgttggga aatgagcgct acttggcgaa agacaagacc ttaacctaca
     2461 cgaccgaaag gccagtagtg gttgcgtaag gcaagttatg aagagtggtg ggctgataca
     2521 aatctgcttt aagaagtcgg tttggttttc tctcctactc tcatcctgtc ttattctctc
     2581 cggctgcttt gaaatgtcgg aggaaatctg gatcagtacc gacggatcgg ggaggataaa
     2641 agaggacttc ataatttccg atgcactgct tggcttagct caggggcgaa gtcaggatga
     2701 tcttattgga cgtgtgcgag agcggtacga ggaagccaaa aaggacttag aaaagaattc
     2761 tttcgtaaag cgggttgaat tccgggaata cagcgaaggg ggaatgcacc attttagtct
     2821 tgacgtcacg gtcaggcaat tccagaatct caacgaaatt cagaagaaaa ttccccccta
     2881 tatagaagat tccggcgaag aatccgactt tcgcatagag aagttgccca gcggcaatat
     2941 ttccttccgg caaaaattcg gcgaatcccc ggcagaaagc acggaagcag aggcggatac
     3001 atccgaacgc aacccctggc tgaaattcgg cgaattcatg gcgaaagcgt cgatttctac
     3061 cgcctttggg aacaggtact ttaccgtgcg gcttcatgcg ccgaaaattg tttccacgaa
     3121 cggaaactta gacgaccgga tgcaaacggc ggagtggaaa cttgccttag ttgacctttt
     3181 gagtgaaaac ccctatgaac aagaactgaa agccgagatg catccgcccg ggaaagggct
     3241 tttgtggatt gttgcggcaa ttcttttggt ggccgttatt ggcggcatta cattagttac
     3301 tatgagaagg aggaagagag gtgttgaacc aatggccccg gcggggaact gatcctgcct
     3361 ggggtgggtc gaacctcaga aaagccggtg gcgattgcgt gaaagggtag cctgagtgtt
     3421 cactaccggt ttgtcggaaa tcgtgttggt tgtgaaagac gtagctgcct cagcccggtt
     3481 ctaccgtgaa gtagtggggc tgacgctcaa tgaagccgcc gcctccgata agaattgggc
     3541 ctggttctgg gctggcgtac ccggccatcc gcaatcggtt gcggtaactc aggataagga
     3601 cctgctcttt gcggaaaagt caccgctgcc ggaaggccaa cgctggggac aggtgcatta
     3661 tgctttcaac gtgccccgcg aaaaattagc cgaggcggtt gagcacgtgc gcgggaaggg
     3721 agtggaagtt tacgggccga aacattttaa gtggatgaaa gccaccgcct actatttcta
     3781 tgatccggac ggcaacctgc ttgaattctg gtctccggac tgaacatgag agaaagcggg
     3841 gaagaccttg acctacacga cttgtggtga acgtagttga accacggaga ggccggtggt
     3901 ctaaacatta agttaaatgg tttgcaggga gaaacttaac ttttggtgaa tatgttctca
     3961 cgtaagagaa ttgttaaaat tggcattgta agtattcccg gtaagagacg agagaagaac
     4021 agattaaagt atgttagtgc ccattatgag caagcatttt tttccgtaag agatagttta
     4081 agcggcaaat atccagtaga acagattaac ctcaaaaaat tagcaaaatt cgagcgacta
     4141 tttaaacgtg gaattcaaat attagtgctg cctgaagctg gggttattac tcatcaacaa
     4201 ttaaataatc ttgaagcctt tgtaaaactt gggggtatag tcttcgccga ttatccatct
     4261 cttgcctttg acgctcttga tac
//
LOCUS       DBNGZA010000127         8442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1719561, whole genome shotgun sequence.
ACCESSION   DBNGZA010000127 DBNGZA010000000
VERSION     DBNGZA010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1719561"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2556
                     /locus_tag="AC16FU_02830"
     CDS             109..2556
                     /locus_tag="AC16FU_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013524.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HIR2993341.1"
                     /translation="MEKWYKVISGLVALFLFGGLCFSNNAVAEETSFRFSFVSVDRNG
                     LNQTSAGVELPGDYLRMAEPGLPALPYRLVHLLLPAGMEVESLSVSCDAPQLLDGLFA
                     IPPAPERIAPDGRTPASDGRDSESKVYAADEPFPGKYAELKSQGIFDGNHIATLALYP
                     YQYLPFEKRVSFTSELQVTLITRPGSLTEVQRISTNSPAQWFVTQPTAGAKTPVSGGT
                     ALSGVQGQSSGCPLGAAYLLVTGARLADAFAPLVEWKKKKGLSAATALIDSVVKHYPG
                     RDDAERLRNYLIACYQSGTVWVLLGGDETVVPIRYAFYTDAAAAPPSTDLAICDLYFA
                     DVNGNWDKDGDGIWGEPTQDQPDLYPDLFVGRVPAADSIAAANFVEKLLTYERNPGYG
                     DPAYLTRVMHMSSDQMRDWPVGVGQHGLVGAKFPPLFQQDLTTLVEAPAGNAPNPASP
                     DGPFSVQRLSQGQGFVTIFAHGRPDGFFARSHLVAQSPISFVFTWGADGDGHGHLANL
                     NNAGKAGIWYSISCDPTHLDCELPPWNLTVPCLGQMLVTRKQSGAVAFIGHSRWGWIF
                     SSYLLMQKFVESIFTNPQGYHLGVAFALGKISFPAYRDLNYNQLLFGDPEMPIYTAPP
                     RPIALSAPASLPVGYSSFVVQAAADSQPLPQATVVIMNRADSILTVAQTDANGNAAFS
                     LSLPTVEPLTVTATRPNYLPVEKTIQVGGTPVTDNDKFKGVLPTLLGQNAPNPFNPLT
                     VITYQLAKSEPARLEIYNLLGQKVRTLVDVQEKAGAHREAWDGRDEKGLEVASGIYFY
                     RLSTPSFSAARKMTLLR"
     gene            complement(2553..3416)
                     /locus_tag="AC16FU_02835"
     CDS             complement(2553..3416)
                     /locus_tag="AC16FU_02835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993342.1"
                     /translation="MNAPASVSVIIVTWNCAGDIARCLQSLSVEQGLIGEVALIDNAS
                     TDWTVKLVEQQFPQVRVIRHRENLGYAAAVNQGLREVSGELVLLLNPDAWVKEAGLRP
                     LLDYLHKHSDVGACAPRLLFPDGRFQASCRRFPTVGRLLIEASGLGLILPFLSHWRWK
                     LSEKEHQEGPVDQPMTSVFLLKRRTMNQTGLFDESFPIFFNDVDYCRRIWIAGFSIAY
                     VPQAQWYHRLGGATGQRKFRMTFVSHRSFFRYFWKHASLIGRLGLVLLWPFFWLLLFP
                     RLLAAVFVQPD"
     gene            complement(3413..4678)
                     /locus_tag="AC16FU_02840"
     CDS             complement(3413..4678)
                     /locus_tag="AC16FU_02840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2993343.1"
                     /translation="MNRERTALIGILAVGLGLRLLYIFAFPLYQAADEYPHFHYIQHL
                     GLTHALPRSTPQYPEYEGFQPPLYYFLSSLVYAPLARYDRDRESSAPLALILPESERA
                     KPPNSLALFFRVLSTLLAVVTLYVVYLISRNFFPDSAGLRLASVAWVAVLPAYATNTA
                     AITNDALAVLVGALLILFLVRQSGWDSKIAFGFGILTGLGALTKHNLLIFLPAGIVAP
                     FFFQSDKRGKAMLTFGLGFVATTFFWYWHNWQTYGSLLAINPDFPTASLFDVRRAQVL
                     QAVRNLARSFWLAGGINYKVMLPVWVYVVVFGGLSLLALSGWVQCARHQWPEFRQGLK
                     QYRIYLLLAILLIFASLFYSLSYPPGRMTSWGKNLFPLLPLWSFFFVGGWKYFWPRAG
                     LGLVWSCVFLLVIVNLVYLFGCVIPYYST"
     gene            complement(4675..5967)
                     /locus_tag="AC16FU_02845"
     CDS             complement(4675..5967)
                     /locus_tag="AC16FU_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993344.1"
                     /translation="MSGPKKVLMVSYYFPPLGMAGVQRVTKFVKYLPEFGWQPVVLTT
                     KTIAFPHADPSLFTDVPQATPIYRAGSFDPLRLAYLWGRSFHGRPVKVGRGSRGSIGE
                     WLNLPDNKIGFVPLALAKARRIVRQEKIDLVWTTSPPPSIHGVGLALKTVFRLPWVAD
                     FRDLWSGTFDRSFPSRGHRWLAGRWQKKFVSGADAVVAVSGEMAQRLGESTSEATQIF
                     TIPNGFDPADLPKNVRKKGAPFTIAYWGTVTPKRSFDRFFAALALWRERARLSSERFR
                     FLQIGAALDGDPFAGARRLGLTEAVEAKGYLPHRQGLAELAQADLFLLLIGEGEGSLG
                     VLTGKLFEYLALGKPILALVPPNGEAARLIENLGAGMVVAPHNQAGMVAALEHFHALF
                     LKKKMSSGVAAERLAPFSRLEQTRTLAGLFDRIVAKRT"
     gene            complement(5964..7085)
                     /locus_tag="AC16FU_02850"
     CDS             complement(5964..7085)
                     /locus_tag="AC16FU_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993345.1"
                     /translation="MSAPRTIAILADARSIHTQRWANYFVRRGDKVNLLSLEPGEGDA
                     YTYQRLRSRLPVRGFKYVAAVPSLRQKLSRMKPDIVNAHFVPNYGLLGLLAGFRPLVV
                     TCWGSDVLLSPLKSGLHRRRVERVIRGADFLTSDSAYLTDRLVAFGAFAEKIATVPLG
                     VEDEILALGAERHHSGDEKVVVLSTRQLEPVYDVETVLHTVPKAIMGFPHEMEFWIVG
                     AGSQRRTLETLSHAIGVSLWTKFLGWLERPRYLEVLRQAAVYVSTARSDSTSVSLLEA
                     MGAGLAPVVADIPGNREWITDGENGFLVPVDSYLSFGEKIRFLADRSELRQQFAVKNQ
                     QIIRAKARWQENMQVISELFDRLIGTKGQTFQTAASLST"
     gene            complement(7082..8074)
                     /locus_tag="AC16FU_02855"
     CDS             complement(7082..8074)
                     /locus_tag="AC16FU_02855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2993346.1"
                     /translation="MTVRQFSSATVDQGEWTRLVHSAPEATFYHTFFWTTVWEKTFPS
                     GTAHFFAVEEAGRYRSGLPAVRFSKRGLAEWHSLPFGTYGGVIGGDGATTEDRAICLE
                     AFRQASREKKSRGAILVDFNGTQPLLPGGRATALETEIVHLPAGAPWFNLWTPMARRS
                     YRKAMNLGLQIDYPHGLEDLSACFELVKLRSRRKKTAWNYPLSFYRELFQAHQNPALD
                     PEAKVLWPVARKGEKVAAFQIFFQFRDQLIYWDGASLPEALPLRPNHLLFGSMFDWAS
                     RNGIQTINLGGSPGREKGLALFKRSLGGESYTYRQYRFPTALGKVVERAKKILR"
     gene            complement(8082..>8442)
                     /locus_tag="AC16FU_02860"
     CDS             complement(8082..>8442)
                     /locus_tag="AC16FU_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993347.1"
                     /translation="LTGDLWIAAGSPLSGEEGGLSRFRTGFGGTVSDLKQVEAYPNPF
                     VLESGRELLTFKGLPFEATVTIYNVAGDRIWRRDRTDQWDGRNEGGELVAGGIYLFVA
                     TAPGKEPGVGKVAVIRK"
BASE COUNT         1872 a         2580 c         2378 g         1612 t
ORIGIN      
        1 tgaaccgaac ttgcacgcct gaaaaaaagg ccttgtgcgg gaaagggggc attgcctttg
       61 cagtttcgcc cccttctgga gtcgaaagac tcactagagg aggggaaagt ggaaaaatgg
      121 tacaaagtta taagcgggct tgttgcgctc tttctttttg gtgggctttg cttcagcaat
      181 aatgctgtgg cagaagaaac ttctttccgt ttttcatttg tgtcggttga tcgaaacggc
      241 ttgaaccaga cctcggccgg agtcgaactg cccggtgact acctgagaat ggctgaaccc
      301 ggactgcccg ctctccccta ccggctggta catctcttgt tacccgccgg gatggaagtc
      361 gaaagtctct ccgtttcctg cgacgcgccc caactcctgg acggcctttt tgcaattccc
      421 cccgcgcccg aacgaatcgc gcctgacggt cgaacccccg catcggatgg cagggactct
      481 gaatcaaaag tttacgcagc cgacgagccc ttccccggca agtacgccga actgaagagc
      541 cagggcattt ttgacgggaa ccatatcgcc acgctggcct tatacccgta tcagtatctc
      601 ccttttgaaa agcgagtctc ctttacctcc gaactgcagg ttacgctgat cacccggccc
      661 ggttcgctga cggaggttca aagaatttcc accaactctc ccgcgcaatg gttcgtcacg
      721 caaccgacgg ctggagcaaa aactcccgtg tcgggcggaa ctgctttgtc aggtgtccag
      781 ggtcaatctt ccggctgtcc cctgggcgcg gcctatcttc tggttaccgg cgctcgtttg
      841 gccgatgcct ttgctcctct ggtggagtgg aaaaagaaaa agggtctttc cgccgccacc
      901 gcactgatcg attcggtggt aaagcactat cccgggcgcg acgacgcgga acgtctgcgc
      961 aactacctaa tcgcctgcta ccagtccgga actgtttggg tactcctggg cggcgacgaa
     1021 acggttgtcc cgatccgcta tgccttttac accgacgctg ccgccgcacc accgtcgacc
     1081 gacttggcga tctgcgacct ctacttcgcc gacgtgaacg gcaactggga taaggacggc
     1141 gacggcatct ggggcgaacc gacccaggac cagcccgacc tctatccgga tctgttcgtc
     1201 gggcgtgttc ccgcggcgga ttccatcgca gccgcgaact tcgtcgaaaa actcttgacc
     1261 tacgagagaa atccggggta cggtgaccct gcctacctca cccgggtgat gcacatgagt
     1321 tcggaccaga tgcgcgactg gccggtcggg gtcggccagc acggcttggt cggcgccaaa
     1381 ttcccccctc tctttcagca ggacctgacg acactcgttg aggctcccgc cggcaatgcg
     1441 ccgaatccgg ccagtccgga tggtccgttc tcggtccaga gactttcgca gggtcagggt
     1501 tttgtgacca ttttcgccca cggccggccc gacggcttct ttgcccgctc ccacctggtg
     1561 gctcaatcgc cgatatcgtt cgttttcacc tggggcgccg atggcgacgg gcacgggcat
     1621 ctggcaaacc tgaacaatgc cggcaaggcg ggcatctggt attcgatctc ctgcgacccg
     1681 acccacctcg actgcgaact gccgccctgg aacctcacgg ttccctgttt ggggcaaatg
     1741 ctggtcaccc gcaaacagtc gggcgcggtg gcgttcatcg gccactcccg ctggggctgg
     1801 atcttttctt cgtacctcct gatgcagaag ttcgtggagt caatcttcac gaatccgcag
     1861 ggctaccatt tgggcgtggc ctttgcgctg gggaaaataa gttttccggc ctaccgcgac
     1921 ttaaattaca accagctgtt gttcggcgat ccggaaatgc cgatctacac cgcgccgccg
     1981 cggcccatag ccctttctgc gcctgcgtcc ttaccggttg gatactcctc ttttgtggtt
     2041 caggcggctg cggattccca gccgctgccg caagcgactg tggtcataat gaaccgggcc
     2101 gattcaatct tgacggtggc gcagaccgat gcgaatggaa acgccgcctt ttcgctctct
     2161 ctgccgacgg tcgaaccgct tacggtgacc gctaccagac cgaattacct gccggttgag
     2221 aaaaccattc aagtcggtgg aacgccggtc accgacaacg acaaattcaa aggggtacta
     2281 cctacgctgc tcggccagaa cgctcccaat ccgttcaatc cgctaacggt gatcacctac
     2341 caattggcga aaagtgagcc ggcccggctg gagatttaca atcttctggg tcaaaaggtg
     2401 cggacacttg tggacgtgca agagaaagcc ggcgcccatc gcgaggcgtg ggacggcaga
     2461 gatgaaaaag gccttgaggt tgcgagcggc atttacttct accgcttaag caccccctcc
     2521 ttcagtgcgg cgcgcaaaat gaccttgctg cgttagtccg gttgaacgaa aaccgctgcc
     2581 aggagtcgcg gaaaaagcaa gagccaaaag aaaggccaga ggagtaccag ccccagccga
     2641 ccgatcaagg aggcatgttt ccagaagtag cggaaaaaac tccggtggga aacaaacgtc
     2701 atcctgaatt tcctctgacc cgtcgcaccc cccaaccggt ggtaccactg cgcctgcgga
     2761 acgtaagcaa tggaaaatcc agcgatccag atgcggcggc aataatcgac gtcattgaaa
     2821 aaaatcggaa atgattcatc gaataacccc gtttggttca tcgtcctccg tttgagcaaa
     2881 aagaccgaag tcatcggctg atccaccggc ccctcctgat gctctttttc ggagagtttc
     2941 cagcgccagt gtgagagaaa aggcagaatg agccccagcc cactcgcctc aatcagcagt
     3001 cgcccgaccg tgggaaagcg gcggcaggag gcctggaacc gtccgtcggg gaaaagcaac
     3061 cggggcgcac aggcgcccac atccgaatgc ttatgtaagt aatcgaggag tgggcgcaac
     3121 cctgcttctt tcacccacgc atccgggttg agcaaaagta ccagttcacc cgaaacttcc
     3181 cgcagacctt gattgaccgc tgcggcgtat cccagattct cgcggtggcg gatgacgcgc
     3241 acctgcggga actgttgctc caccagtttt accgtccagt cagtcgaggc attgtcaatg
     3301 agggctactt cgccgatcaa accctgttca actgacagcg actggaggca acgggcgata
     3361 tcccccgcgc agttccaggt gacgataatg accgaaaccg aagccggtgc gttcacgtcg
     3421 aatagtaggg gatgacacag ccgaacaaat agacgagatt aacgattact aacaggaaaa
     3481 cgcacgacca gaccagcccg agacccgccc ggggccagaa atacttccac cctcccacaa
     3541 agaaaaaact ccagagcggc aaaagcggga agagattttt cccccaggag gtcatccgcc
     3601 ccggcgggta ggaaagggaa tagaaaagcg atgcaaaaat taaaagaatt gctaggagca
     3661 ggtagatgcg gtactgtttt aggccttgcc ggaactccgg ccactggtgt cgggcacact
     3721 gcacccaacc cgagagtgcc agaagcgaga gcccgccgaa aaccaccacg taaacccaca
     3781 ccgggagcat gaccttatag ttgatccccc ccgccagcca gaaactgcgg gccaaattgc
     3841 gcaccgcctg gagcacctgc gctctccgga catcgaacag actggcggta ggaaaatcgg
     3901 ggttgatcgc caaaagcgaa ccgtaggtct gccagttgtg ccagtaccag aaaaatgtcg
     3961 tggcgacaaa acccagaccg aacgtgagca ttgccttccc gcgcttgtcc gactgaaaga
     4021 aaaatggagc aactatcccc gccggaagaa aaatcaaaag gttgtgcttg gtgagcgctc
     4081 ccagcccggt gagaatcccg aagccgaaag cgattttgga atcccacccg ctctgccgca
     4141 ccagaaaaag gatgagcagc gctcccacga gcaccgccaa agcgtcgttg gtgatggcgg
     4201 cggtgttggt cgcataggcg ggtaaaaccg caacccaggc cacactcgcc agccgcaacc
     4261 cggcgctgtc ggggaaaaag tttctgctga tgaggtacac cacgtacaaa gtgactacgg
     4321 ccagcaaagt cgagaggact cggaagaaga gtgccaagct gttcggtggc ttggcgcgct
     4381 cgctctccgg cagaatgagc gcaagggggg cgctgctctc ccggtcacgg tcataccgcg
     4441 ctaggggcgc ataaaccaga gatgagagga agtagtagag cggcggctga aacccctcgt
     4501 actccggata ctgaggcgtt gagcgcggca acgcatgggt gagcccgaga tgctgaatgt
     4561 agtggaaatg cggatattca tccgccgcct ggtagagggg aaaggcaaaa atgtagagca
     4621 atcgtaaacc gagtcccacc gccaggatgc ctatcagtgc ggtccgttcc ctattcatgt
     4681 gcgctttgcc acgatgcggt caaaaagccc cgccaaagtc cgggtctgct caagccggga
     4741 aaagggggcc agcctttcgg cggctacgcc gctcgacatt ttctttttca aaaaaagagc
     4801 gtggaaatgc tccagggcag ccaccatccc tgcctggttg tgtggcgcca ccaccatccc
     4861 ggcgccgagg ttttcaatga gccgggctgc ttcaccattc ggcggcacca gagccagaat
     4921 cggtttgccc agagccaggt attcaaagag cttccccgtt aaaaccccca aggacccttc
     4981 tccctcaccg atcaaaagca aaaagaggtc tgcctgcgcc agttccgcca gcccttgccg
     5041 gtggggcagg taccccttcg cttccaccgc ctcagtcaat cccaaacgcc tagctccggc
     5101 gaacgggtcg ccgtccagag ccgcgccgat ttgcaggaac cgaaatctct ccgaactcag
     5161 gcgggcacgc tctcgccaca gagccaaggc ggcgaagaat cgatcgaaag atcgtttggg
     5221 ggtcaccgtt ccccagtatg caatggtaaa gggggctcct tttttccgga cgtttttcgg
     5281 cagatccgcc ggatcgaatc cgttcggaat ggtgaagatt tgagtagcct cgctcgtact
     5341 ttcgcccagc cgctgggcca tttcgcctga gaccgccacc accgcatcgg cgccgcttac
     5401 gaatttcttc tgccaccggc cggccagcca ccggtgtccg cgggagggga aactccggtc
     5461 gaacgtaccc gaccagagat cgcggaagtc ggccacccag gggagacgaa aaactgtctt
     5521 gagagccaat ccgacgccgt ggatcgaagg cggtggcgag gtggtccaga ccagatcgat
     5581 tttttcttgc cggacgattc ggcgtgcctt ggccaaagcc aggggcacaa agccgatttt
     5641 gttgtcggga agattcaacc actccccgat tgaaccccgg ctccccctgc cgaccttcac
     5701 gggccgtccg tgaaaactgc gcccccagag ataggccaga cgcaagggat caaaggaacc
     5761 ggcccggtaa atcggcgttg cctgcggaac gtcggtgaaa agcgacgggt cagcgtgggg
     5821 aaaggcaatc gtcttggtcg tcaacaccac cggctgccag ccgaactccg gcaggtactt
     5881 gacgaatttg gtgacccgct gcacgccggc cattcccaga ggcgggaagt agtagctgac
     5941 catcaagact tttttcgggc cactcatgtg gaaagagatg cagcggtctg gaaggtttgc
     6001 cctttggttc cgatcaggcg gtcgaagagc tccgaaatca cctgcatgtt ctcctgccag
     6061 cgggccttcg cacggataat ctgttggttc tttaccgcga attgctggcg caactcggat
     6121 cgatcggcga ggaagcggat tttctcacca aagctgaggt agctatcgac cggaacgaga
     6181 aaaccatttt caccgtcggt gatccactcc cggttgccgg ggatgtcggc aaccaccggc
     6241 gccaatcccg cgcccatggc ttccaaaaga ctaaccgagg tggaatcgga acgcgcggtg
     6301 gaaacataca cggcggcctg gcgaagaacc tcgagatagc ggggccgttc cagccacccc
     6361 agaaactttg tccagagcga aacgccgatc gcatgcgaaa gggtctccag ggtgcgtctc
     6421 tggctccccg caccgacaat ccagaactcc atctcgtgcg gaaagcccat gatcgctttg
     6481 ggtaccgtat gcagcaccgt ctcgacgtca taaaccggct ctaactgccg tgtggagagc
     6541 accacgactt tttcatcacc ggagtggtga cgctctgctc ctaaagcaag aatttcgtct
     6601 tcgaccccca gaggcaccgt ggcaatcttc tctgcaaaag ctccgaacgc caccagccgg
     6661 tcggtgagat aggcagagtc ggaagtcaaa aaatcagcgc cgcggatcac ccgttcgacc
     6721 cgccgccggt ggagaccaga tttgagcggt gaaagcagca cgtccgaccc ccagcaggtc
     6781 accaccagtg gccggaaacc agcaaggaga cctaacagac cgtaatttgg aacgaagtgg
     6841 gcattgacga tatcgggctt catccgggat agcttctgcc tgagagaggg cactgccgcg
     6901 acgtatttga aaccccgcac cggcagcctc gaccgcaagc gctgatatgt gtatgcatcc
     6961 ccctctcccg gttcaagcga aagcaggttg accttgtcgc cccgccggac aaagtagttc
     7021 gcccagcgtt gcgtatggat gctccgcgcg tccgcgagaa ttgctatagt gcggggagcg
     7081 ctcatcgcaa aatctttttg gcccgttcca caacctttcc cagagctgtc ggaaaacggt
     7141 actgccgata ggtatagctc tcccctccca gggagcgttt gaatagagcc agtccttttt
     7201 cccgtcccgg cgagccgccg aggttgatgg tctggatgcc gttgcggctg gcccagtcaa
     7261 acatcgatcc gaacaaaaga tggttgggtc gcagtgggag tgcttccggc agactcgccc
     7321 catcccagta gattaattga tcccgaaact gaaagaaaat ctggaatgcc gcaaccttct
     7381 caccctttct cgccaccggc cagaggactt ttgcttccgg atcgagcgcc gggttctggt
     7441 gcgcctggaa caattcccgg tagaaagaca aaggataatt ccaggcagtc tttttgcgcc
     7501 ggctccgcag tttcaccagt tcaaagcagg cgctgaggtc ttccagtccg tgcggataat
     7561 caatttgcag tcccaaattc atcgccttcc ggtaactccg ccgcgccatg ggagtccata
     7621 gattgaacca gggtgctccc gccggcagat gcacgatctc ggtttcgagt gctgtggctc
     7681 gcccgccggg caaaaggggc tgggtgccgt tgaaatcgac aaggattgct ccgcggcttt
     7741 tcttctcccg gctggcctgc cggaatgcct ccaggcagat cgcccgatcc tcggtcgtcg
     7801 cgccgtcacc gccgatgacc cccccgtagg tgccgaacgg cagggagtgc cactccgcca
     7861 ggcctctttt ggaaaaccgc accgccggta gcccggagcg gtaccgtccg gcctcttcca
     7921 ctgcaaaaaa atgagcggtg ccggagggaa aagttttttc ccatacggtc gtccagaaaa
     7981 aagtgtggta gaaggtcgcc tccggtgcgc tgtgaaccag ccgggtccac tccccctgat
     8041 ccaccgtcgc agaagagaat tggcgaaccg tcacgtacgg atcacttgcg aatgaccgcg
     8101 accttgccca cccccggttc tttcccggga gcagtggcga cgaagaggta aatgccgccc
     8161 gctaccagtt cccccccctc gttgcggccg tcccactggt cggtccggtc ccgccgccag
     8221 atccggtcgc ccgcgacgtt gtaaatcgtc accgtcgcct caaacggcaa tcctttgaac
     8281 gtcaaaagtt cgcggcccga ctcaaggacg aagggattcg ggtaggcttc cacctgttta
     8341 aggtccgaaa ccgtgccacc gaatccggtg cggaaacgcg acagtccccc ttcctcaccg
     8401 gagagaggcg agccggcggc aatccagagg tcgcccgtaa gg
//
LOCUS       DBNGZA010000128         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_872264, whole genome shotgun sequence.
ACCESSION   DBNGZA010000128 DBNGZA010000000
VERSION     DBNGZA010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_872264"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="AC16FU_02865"
     CDS             <1..1558
                     /locus_tag="AC16FU_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2993348.1"
                     /translation="SRELFPQEQRHPGETNMSDPRSEFPSSAAVEPSHPDPRQRRDKS
                     SDLSENMRRQLVVQMYVIFRTAQLYEPANSAYQKALEALLHTLSECFAIEGEAQIVFR
                     QGYWYVNGARTRFEIDGRTAAESLAQLFEQRQIGGMGFEPAVSREELVRFADLFLHTE
                     APTPEERLALLMKKLDAAQVLTIILFSPNVSPAEGLADLLTLESSRRSARRTFFYAVT
                     MVEGLMQRAQHGESVNFLKAKRVVHSIIDHLLRDEGALVELATLKTYDDYTYAHSVNV
                     SVLSLALGLRVGLDRHNLSLLGFAALFHDVGKTRLPVELIHKPTEYDEMDWRQMEQHP
                     ILGAKAIALTRAFDEYTARGMLVAFRHHTNQDLSGYPKLRQPRASDLFTRIVKIADSY
                     NAMTSGRAYIKESNTPDEAIRKMLLVSGKAYDSLLLKVFVQVLGIFPVGSLVLLDTQE
                     LALVFRANKSDLSRPQVRLIADATGLKEGLVVVDLAERESPEGPYRRSIVRVLDPKKY
                     GLDLSRFALN"
     gene            complement(1813..>2185)
                     /locus_tag="AC16FU_02870"
     CDS             complement(1813..>2185)
                     /locus_tag="AC16FU_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR2993349.1"
                     /translation="AFTLAFVALPLVLAADKDLKTVTLSVKGMTCAGCKKGVESALLK
                     ANGVEAASVDLETNTATVTYDGSATTVKRVMGSVKKAGFKAEMKNKEISETDCGPCPH
                     DVKATTSTNGKACCTAKKQGT"
BASE COUNT          433 a          666 c          624 g          462 t
ORIGIN      
        1 gtccagggag ttgttcccgc aagaacagcg gcatcccggg gagacgaaca tgagtgaccc
       61 gcgctccgaa tttccgtctt cggccgccgt cgagccgtca caccccgatc cccgtcaaag
      121 gcgggacaag agttcggatc tctccgagaa catgcgccgg caactggtgg tgcagatgta
      181 cgtcatcttc cgcaccgccc agttgtacga gccggcaaac agcgcctacc agaaggcgct
      241 ggaggcgctc ctgcacacgc tgagtgaatg cttcgccatc gaaggcgagg cccaaatcgt
      301 cttccgccag gggtactggt acgtgaacgg cgcccgcacc cgttttgaaa ttgacggtcg
      361 gaccgccgcg gagtctctgg cacaactttt tgagcaacgt caaatcggcg ggatgggatt
      421 tgaaccggcg gtcagccggg aggagctcgt ccgctttgcc gatctgtttc tccacaccga
      481 ggcacccacg cctgaggaac gtctggcgct tctgatgaaa aaattagatg ccgcccaggt
      541 gttaaccatt atccttttca gccccaatgt gtccccagcc gagggcttgg ccgacctttt
      601 gaccctcgaa tcgagccggc gttcggcccg gcgcaccttc ttctacgcgg tcaccatggt
      661 tgaaggcttg atgcagcgcg cgcagcacgg ggagtcggtt aacttcctca aagccaagcg
      721 agtggtgcac tcgatcatcg accacctctt gcgcgatgaa ggtgccttgg tcgaactggc
      781 gacgttgaag acctacgacg actataccta cgcccattcg gtgaacgtga gtgtcctctc
      841 tctggcgttg ggcctccgcg tggggctgga ccgccacaat ttgagtcttc tggggtttgc
      901 ggcgctcttc cacgacgtcg gcaagacgcg gctgccggtg gagttaatcc ataaaccgac
      961 cgaatatgac gagatggact ggcggcagat ggaacagcat ccgatcctgg gcgccaaagc
     1021 catagcgctc acccgcgcct ttgacgagta caccgcgcgc gggatgctcg tggctttccg
     1081 gcaccatacg aatcaagatc tctcggggta tcccaaactc cgccagcccc gggcgtcgga
     1141 tctcttcacc cggatcgtca aaatcgccga ttcgtacaac gccatgacgt cgggacgggc
     1201 ctacatcaaa gaatcgaaca cgccggacga agcgatccgc aaaatgcttc tggtttcggg
     1261 caaggcgtac gattccctcc tgctcaaggt gttcgtccag gtgctgggca tttttccggt
     1321 ggggtccctg gtgctcctgg acacgcagga actggccttg gtcttccgcg ccaacaaaag
     1381 cgacctctcc cggccccagg tgcggctcat tgccgatgcc accggcctca aagaaggtct
     1441 ggtggtggtc gatctggccg aacgggaatc tcccgaaggg ccctaccgcc gcagcatcgt
     1501 ccgcgttttg gatccgaaga aatacggcct cgatctttcc cggttcgccc tcaattagca
     1561 tctgatttca ggaagttcca atccggtacg aaagcttccg agatttaagg gtttatacgc
     1621 cggcaatctt caggcgactg cagccgcttt ttattccata gaggtcaaga atcccgcctg
     1681 acccctcctt cgcccctcct ttggagagat ggaagaaaaa gggagaaaaa aatagcgcgg
     1741 ggttgtgaat gaaccccgcg ctgcgaactc gaatagtagc gcgactcacg tcgcgctacc
     1801 ccttagaagg gattacgttc cctgtttttt cgccgtgcag caggccttgc cattcgtgga
     1861 cgtggtggct ttcacgtcgt gcgggcaggg gccgcagtcg gtttcggaaa tctccttgtt
     1921 tttcatttcg gccttgaagc cggctttttt caccgatccc atcacgcgct tcacggtggt
     1981 ggcgctgcca tcatacgtga ccgtggcggt attggtctcc aggtcgacag aggcggcttc
     2041 gacgccattg gcctttagaa gcgcgctctc cacgcctttc ttgcagccgg cgcaggtcat
     2101 ccccttgacg cttaaggtga cggtcttcaa atctttgtcg gcggccagta ccaaaggcag
     2161 cgcgacaaaa gccagcgtga aagca
//
LOCUS       DBNGZA010000129         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_235584, whole genome shotgun sequence.
ACCESSION   DBNGZA010000129 DBNGZA010000000
VERSION     DBNGZA010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_235584"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="AC16FU_02875"
     CDS             complement(<1..435)
                     /locus_tag="AC16FU_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993350.1"
                     /translation="MRRTILIVLWTCSFLMMGGRRCPPYGFAGTALAQEDPFDQALAK
                     VGLTRETLRFDDDDMANFGGDKFLLPLFTTLHRNPLKIPDYTRVFRDAVIQNAGSLEN
                     MLTFASLRLKAGVRRNLVANPIAGAVEKSKEPEALYQSILALY"
     gene            complement(443..1201)
                     /locus_tag="AC16FU_02880"
     CDS             complement(443..1201)
                     /locus_tag="AC16FU_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR2993351.1"
                     /translation="MRLVKITLWQKEADGDLSAATLSEISSTRPDFLCLPEYCNASRE
                     TRHQFDIASRFPQYRLTMVEITRRLQTTVIGGSAVEQVGDRYYNTCIVASRGKELGGY
                     HKIHLFAREQGKGLATGNEYKVFEAGGVRFGVLICADVLYEESFLEMRQLRPKLIFIP
                     TTSPFKPNDTLEQKLARDKEIFQRGAELSQAYVVKVCAVGTLFGGRLQGRSLVAAPWG
                     ILWRVSPEDEEKELIQAYELDLDQLRHWQTERQP"
     gene            complement(1241..>1707)
                     /locus_tag="AC16FU_02885"
     CDS             complement(1241..>1707)
                     /locus_tag="AC16FU_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR2993352.1"
                     /translation="RFARGASTLTQQLTKNLYLSTSKNPLRKLRELFIALALEKHLSK
                     RRIFELYLNNIEWGRGIYGVEAAALNYFNKSATDLSPDEAARLAAVIPDPLRLTPFDS
                     SRRFEQKRKLILTRMEQYGFLTHEEYAQALAPVARESDTRAGRVGDSALEPQ"
BASE COUNT          357 a          467 c          511 g          372 t
ORIGIN      
        1 gtagagagcc agaatcgact ggtagagcgc ctccggctct ttggattttt cgaccgctcc
       61 cgcaatcgga ttggcgacca gatttcgccg cactccggct ttgagccgca gggatgcgaa
      121 ggtgagcata ttttccagag aaccggcgtt ttggataacc gcgtcccgaa aaacgcgcgt
      181 gtaatcggga atcttgagcg ggttgcggtg gagcgtggtg aagagcggca agaggaactt
      241 atcgccgccg aaattggcca tgtcatcgtc gtcaaagcgc agcgtttcac gcgtgaggcc
      301 gactttcgcc agcgcttggt cgaaaggatc ctcctgcgcg agcgcggtac cggcaaaccc
      361 gtagggcggg catcttcgcc cacccatcat taaaaacgag cacgtccaga gaacgatcag
      421 gatggttcgt cgcatagatt cctcagggtt ggcgttcggt ttgccagtga cgcagttggt
      481 ccaaatcgag ttcgtaggct tgaatgagct ctttctcctc gtcttccggt gacacgcgcc
      541 agaggatgcc ccagggcgcg gcaaccagac tgcgcccctg aagccgcccg ccgaagagcg
      601 tgccgacggc acagactttg accacgtacg cctggctcaa ttcggccccc cgctggaaaa
      661 tttctttgtc gcgggccagt ttctgctcca gcgtgtcatt cggcttgaag ggcgaagtgg
      721 tcgggatgaa gataagcttc ggccgtagct gacgcatctc cagaaacgat tcttcgtaga
      781 gcacatcggc acagatcaat accccgaaac ggacgcctcc ggcttcgaag accttgtact
      841 cgttgccggt cgccagtcct ttcccctgct cccgggcaaa gaggtgaatt ttgtggtagc
      901 cacccaattc ctttccgcgg ctggcaacga tgcaagtatt gtagtagcga tcgccgacct
      961 gctctaccgc actgccgccg atgacggtgg tctggagccg tctggtgatt tctaccatcg
     1021 tcaaacgata ttgaggaaac cgggaagcaa tgtcaaactg gtggcgggtt tcccgggaag
     1081 cattgcagta ttccgggagg cataagaaat ccggtcgggt actcgaaatt tcagagagag
     1141 tggccgcaga caaatctcca tccgcctcct tctgccagag cgtaattttc actaagcgca
     1201 cgctgaccta ctgcccgcct gcaaacaggg tgtctagagt ttattggggt tcaagagcgg
     1261 aatcgccgac ccttcccgct cttgtatctg actccctcgc gaccggagca agcgcttggg
     1321 catactcctc gtgagtaaga aaaccgtact gctccatgcg cgtcaaaatt aattttcgct
     1381 tctgctcgaa gcgccgggaa gagtcgaacg gtgtcaggcg gagcggatcg gggattacgg
     1441 cggccaggcg ggctgcttcg tcgggcgaga ggtcggtcgc cgatttgttg aagtagttga
     1501 gcgcggcggc ctcgacgccg taaatgcctc tcccccattc gatgttgttc agatacagtt
     1561 caaaaatgcg gcgcttggaa aggtgtttct ccagggccag ggcgatgaac aattcgcgca
     1621 atttgcggag gggatttttt gaagtggaga gatacaagtt tttggtcaac tgctgggtga
     1681 gggtagaggc gccgcgcgcg aagcgct
//
LOCUS       DBNGZA010000130         2591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1826770, whole genome shotgun sequence.
ACCESSION   DBNGZA010000130 DBNGZA010000000
VERSION     DBNGZA010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1826770"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC16FU_02890"
     CDS             <1..789
                     /locus_tag="AC16FU_02890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR2993353.1"
                     /translation="PTLDREVAGTLANTLRKRGIEIFTGHKVTQTKLEKNKATLIVEP
                     AAGATPPFSIECDMVLVAVGLRGNVSSFGLEQTGVQADRHGFITVNGRLETNVPDLYA
                     IGDVVGGKLLAHKASHEGIIAAEAASGRKVEMDYRVVPYAVFTDPEIGAVGLTEEEAR
                     AQGYNLKIGRFPFRVLGKAIGISRTEGFVKIIGDAETDELLGMHIIGAHAGDLIAEGA
                     MAMAMSATCEDVASTIHVHPTLAEAVMEAALDADQRVIHLISKK"
     gene            921..1310
                     /locus_tag="AC16FU_02895"
     CDS             921..1310
                     /locus_tag="AC16FU_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993354.1"
                     /translation="MRKTGLLLPLLVVGLFSCVFGDTPKPGSADAQKKPEVKLTGEVI
                     CLSCYMDHNSKGAQHASCAQMCAKNGVPLALLEDKTENVYPLVKGHEGGNADLAPYAG
                     KRVTLTGHWLEKGGAKIFTFAEVTPAK"
     gene            1452..>2591
                     /locus_tag="AC16FU_02900"
     CDS             1452..>2591
                     /locus_tag="AC16FU_02900"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR2993355.1"
                     /translation="MAKDIVVLDGARTPFGEFCGSLRSVSATELGVSAANAALQRSGI
                     KKEWIEHVVFGNVMQTSGDAIYFARHIGLKCGIPIPVPAVTVNRLCGSGLQAIIQGAQ
                     LINLGEAEFVLAGGAENMSQSPHVIRGARWGLDLGRGQMEDALWVSLVDSYNNMGMAI
                     TAENLAERYKISREQVDEYALRSHSAAAKAQSNGRFTEEIVPVEIKNRKGDVTVVDKD
                     EHVRNDTSLEVLAKLKPVFKKEGVVTAGNASGINDGAAAVILTTKEKAKELGLSYLAE
                     MKTWSYVGCDPDVMGIGPAPAIRQVLKRARLDLEQIDLIEINEAFSAQYLAVEKELDL
                     ERNKVNVNGGAIALGHPLAASGARLTLTLLYELKRRIKKYGIASLC"
BASE COUNT          602 a          710 c          787 g          492 t
ORIGIN      
        1 ccgaccttgg accgggaagt ggccgggact ttggcgaaca ccctgcgcaa gcggggaatc
       61 gaaattttta ccggccacaa ggtgacacag acgaaactgg agaagaataa agcgactttg
      121 atcgtcgagc cggccgccgg agccacgccg cctttttcga ttgagtgcga catggtgctg
      181 gtggcggtcg gcttacgggg gaacgtctcc agtttcggtt tggaacaaac cggcgtgcag
      241 gcagaccggc acggtttcat caccgtcaac ggtcggttag aaacaaacgt gccggacctc
      301 tacgccatcg gcgacgtggt cggcgggaaa cttttggccc acaaggcttc gcacgagggg
      361 attattgccg ccgaagccgc ttccggccgg aaagtcgaaa tggactaccg ggtcgtccca
      421 tacgccgtct tcaccgatcc ggaaatcggg gcggtcggct tgaccgaaga ggaagcgcgc
      481 gcgcaggggt acaacctaaa aatcgggcgc ttccccttcc gggtgctggg aaaagcgatc
      541 ggcatctcgc gcaccgaggg gttcgtaaaa attatcggcg atgctgaaac cgacgaactc
      601 ctgggtatgc acatcatcgg ggcgcacgcg ggcgatttaa tcgccgaagg agcaatggcg
      661 atggcaatga gcgccacctg cgaggacgtg gcttcaacga tccacgtcca ccccactctg
      721 gccgaagcgg tgatggaggc ggccctggac gccgaccagc gggtgattca tctgataagc
      781 aaaaagtaaa agccggcgac ctgaataccc cagtacgttt ttccgtaatt agcttatcga
      841 tttctcccgt gccggcgtat taacggtagt cagcactggt acgaagcacg acaaccgaga
      901 aaagaaagga agaggaaaag atgagaaaga caggactctt gctcccgctt ttggtcgtcg
      961 gtctcttttc ttgcgtcttc ggcgacacgc ccaaacccgg gtcggctgac gcgcagaaaa
     1021 aaccggaagt aaagctcacc ggtgaggtga tctgcctctc ctgctacatg gaccacaact
     1081 ccaagggggc ccagcacgcc tcgtgcgcgc aaatgtgtgc taagaacggc gtccccttgg
     1141 cacttttaga ggacaaaacg gaaaatgttt acccgctggt gaagggacac gaaggcggca
     1201 atgcggatct cgctccctat gcgggcaagc gagtcacgtt gaccggccac tggctggaaa
     1261 aaggcggagc caagatcttc acctttgccg aagttacccc ggcgaagtaa aaagcattct
     1321 tccgccacgg gtgggagtcg cgctctcacc cgtttttttt cagttaccga tcgaagcggg
     1381 acttgacttc tgcggacggt atgctactat ttcgttgaac aaagataaag agaggatgta
     1441 aaggatagaa tatggctaaa gacatcgtgg tattggacgg cgcgcggacg ccgttcggcg
     1501 agttctgcgg atcgctccgg tcggtttcgg ccaccgaatt gggcgtcagt gccgccaacg
     1561 cggcgctcca gcgctcgggc ataaaaaagg agtggattga gcacgtcgtc ttcggcaacg
     1621 tgatgcagac ctccggcgat gcgatctact ttgcccgaca catcggcttg aagtgcggga
     1681 ttcctattcc cgtccccgcg gtgacggtga accgtctctg cggctccgga ctgcaggcga
     1741 tcattcaggg ggcgcagttg attaacttag gcgaagccga gttcgttctg gccggcggcg
     1801 cagagaatat gagccagtcg ccccacgtga ttcgcggcgc gcgctggggg ctcgatttag
     1861 gccggggaca gatggaagat gcactctggg tgtctttggt cgattcctac aacaacatgg
     1921 ggatggcgat tacggcggag aacttagccg agcggtacaa aatttcccgc gagcaggtgg
     1981 atgaatacgc gctgcgttcg cattccgccg cggccaaggc gcaatccaac ggccgcttta
     2041 ccgaagaaat cgtgccggtg gaaattaaaa accgcaaagg cgacgtgacc gtggtggata
     2101 aagacgagca tgtgcgcaac gatacctccc tggaagtgct ggcgaaactc aaaccggtgt
     2161 tcaaaaaaga gggcgtggtg accgccggga acgcctccgg catcaacgac ggcgccgcag
     2221 cggtaatctt gaccaccaag gaaaaagcaa aggaactcgg cctctcctac ctggcggaaa
     2281 tgaagacctg gtcgtatgtc gggtgcgacc ccgacgttat gggcattggg ccggccccgg
     2341 ctatccggca ggtcctcaag cgggcgcggc ttgatttaga acaaatcgac ttgatcgaga
     2401 tcaatgaggc cttctccgcg caatacctgg cggtcgagaa agaactcgat ttagaacgca
     2461 ataaagtaaa cgtgaacggc ggggccatcg ctttaggcca tcctctggcc gcctccggtg
     2521 cacggttgac tttgacgctc ctctacgaac tcaagcgccg gattaaaaaa tacggcatcg
     2581 cttccctctg c
//
LOCUS       DBNGZA010000131         3562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2782422, whole genome shotgun sequence.
ACCESSION   DBNGZA010000131 DBNGZA010000000
VERSION     DBNGZA010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2782422"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="AC16FU_02905"
     CDS             <1..773
                     /locus_tag="AC16FU_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR2993356.1"
                     /translation="NGLLRPDMIVRLPGGREIVIDSKVPLQAFLDAAESKNDPERNAA
                     LLRHAAQVREHMKKLSTKAYWEQFEKTPDFTVMFIPGESFFGAALEVDKSLIEQGAQS
                     NVILATPTTLIALLRTVAYGWRQEQIAQNALQISALGSEIHERISKFIEHFQKVGNAV
                     EKATDAFNAAAGALETRVLVTARKLKELGATSAPEIPLVGQIERLPRSVAIPAESEMV
                     TRLQGRSRVKLSKPSSSTESDEQLMLPAEPDNQAGAAP"
     gene            831..1811
                     /locus_tag="AC16FU_02910"
     CDS             831..1811
                     /locus_tag="AC16FU_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR2993357.1"
                     /translation="MRLQATLLVLLSAVFSLCISVFAQEAKVGHGSLTLDGLVQVWYD
                     YTPDRSQKNGDTFRLRRTEFKFSGIVKPDRIKYFLLLDPASVPSTLIQDAGVAYAFPF
                     GVEVHVGQEKAPVSEEGLRPTPQVDFVEKSILALTFGEQRNIGLQVWGQKKRGEVRLA
                     VINGTGRNQQDNNRNKDFVGRLVVKPVPEISLGASGYTGRSTIGDSLRFKKWSVGGEA
                     GLDWRNWRLRGEILRGHGGKDDKNQTQEGVSGYLRYTYQEKIQAAVRVERWDVNLHQG
                     GDRQDVVTLGAGYLFDSFHARVLANFLVKQEEQGHRISNNEMQVLLQEAF"
     gene            1834..2466
                     /locus_tag="AC16FU_02915"
     CDS             1834..2466
                     /locus_tag="AC16FU_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR2993358.1"
                     /translation="MRTPGQTKIKKILALGFTGLALALFIAARERLPASGQVARVVDG
                     DTIVLESGEHLRYIGIDSPEHDDTLFAEARTLNQKFVEGKTVRLEYDVTKRDGYGRLL
                     AYVWLDQQMVNLELVKAGLARVYTFPPNVRYAQRFMAAQKEARSRKAGMWGIPVWSLE
                     AYYVGNSRSLKFHRPQCRQARRLKSSEKVKFSSRDAALDSGYAPCRNCKP"
     gene            2656..3039
                     /locus_tag="AC16FU_02920"
     CDS             2656..3039
                     /locus_tag="AC16FU_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993359.1"
                     /translation="MFVVTLEVVDHPSGGEVGMSQSAVEFFVVGAVGVRQRRIVARGT
                     RRDEGMLNRRGAEGFFEGTESLDVTEEAAGEVGTMVGRDGLDAERIEAYQSASGGKAT
                     AVWGVSSGYRETNCKRVNPPTAENW"
BASE COUNT          777 a         1015 c         1094 g          676 t
ORIGIN      
        1 agaacggcct tttgcggccc gatatgatcg ttcgccttcc cggcgggcgg gaaattgtca
       61 ttgattccaa ggtgccgttg caggcgtttc tggatgcggc ggagtccaaa aacgacccgg
      121 agcgaaatgc cgccctgctc cgccacgccg cgcaggtgcg cgagcacatg aagaaactga
      181 gcaccaaagc ctactgggag caattcgaga aaacgccgga cttcaccgtc atgttcatcc
      241 cgggcgagtc gttctttggc gccgccttgg aagtggataa atcgctcatc gaacagggcg
      301 cgcagagcaa cgtcatcctg gcgacgccca caacgctcat cgcgctcttg cgcaccgtgg
      361 cctacggctg gcgccaggag caaatagctc agaatgcgct ccagatcagc gctctgggga
      421 gcgaaataca cgagcgcatt tcgaagttta tcgaacattt ccaaaaagtg ggtaatgcgg
      481 tcgagaaggc gaccgacgcc ttcaacgcag ccgcgggggc tttggaaacg cgagttttgg
      541 tcacggcgcg aaaattgaaa gaactgggtg ccaccagcgc acccgaaatt cccctggtgg
      601 ggcagatcga acgattgccc cgctcggtgg ccatacccgc cgaaagcgaa atggtgacaa
      661 ggctacaagg tcgttcacga gtcaaacttt ccaaaccgag ttcatctacg gagtcggacg
      721 aacagctaat gctccctgcc gaaccggaca accaggcggg cgccgcgccg tagcgaaaac
      781 tttctaacct gacattccat ttgcatcatc ccgaacgaaa ggactcgccc atgcgcctgc
      841 aagccacatt gctcgtcctg ctcagtgcag ttttttccct ctgcatttcc gttttcgccc
      901 aggaggcgaa ggtcggccac ggcagtttga ccctcgatgg gctcgtccag gtttggtacg
      961 actacacccc cgaccggtcg caaaagaacg gcgacacctt ccgcctgcgc cgtaccgaat
     1021 tcaaattctc aggcatcgtc aagcccgacc ggatcaaata ttttctgctc cttgatccag
     1081 cttcggtgcc gtccactctg attcaggacg cgggcgtggc ctatgccttc ccctttggcg
     1141 tggaggtgca cgtcggccag gaaaaggcgc cggtctctga agaggggctg cggcccacac
     1201 cccaggttga tttcgtcgag aaatcgattc tggctctgac cttcggcgag cagcggaaca
     1261 tcggcctgca ggtctgggga cagaagaaac ggggagaagt gcgtttggcc gtcatcaacg
     1321 gcaccggccg gaatcagcag gataataatc gcaataaaga tttcgtcggg cgcctcgtcg
     1381 taaagcccgt gccggaaatt tccctgggtg catccgggta cacggggcgc tcgaccatcg
     1441 gggattccct gcgttttaaa aagtggagcg tgggcggcga agccggcctt gactggcgca
     1501 actggcggct gcggggcgaa atcctccgcg gccacggcgg caaggacgac aagaatcaaa
     1561 cccaggaagg cgtttccggc tacctccgct acacctatca ggaaaaaatt caggccgcgg
     1621 tgcgggtcga acgctgggac gtcaacctcc accagggcgg cgaccggcag gatgtggtga
     1681 ccctcggcgc ggggtacctc ttcgacagtt ttcacgccag ggtattggcc aactttttgg
     1741 tcaaacagga agaacagggt caccggataa gcaacaatga aatgcaagtg ctgctgcagg
     1801 aagcgttcta ataccagaac acagaacacg ggcttgagaa caccgggcca gacaaaaatc
     1861 aaaaaaattc tggcactcgg cttcaccggg ctggcgttgg ctcttttcat tgcagcccga
     1921 gaacgcctgc cagcgtcggg gcaggtcgcg cgggtcgtcg acggcgacac catcgtcctc
     1981 gaatcgggcg agcacctccg ctacatcggc atcgactcgc cggagcatga cgacacactc
     2041 ttcgccgaag cacgaacatt gaatcaaaaa ttcgtggaag ggaaaaccgt ccggctggaa
     2101 tatgacgtga ccaagcggga tggttacggg cgccttttgg cctacgtctg gctcgatcaa
     2161 cagatggtca atctggaact ggtcaaagcc ggattggccc gggtctatac ctttccgccc
     2221 aatgtccgct acgcccagcg cttcatggcc gcgcaaaaag aggcgcgcag ccgcaaagca
     2281 gggatgtggg ggattcccgt ctggtcgctg gaagcatact acgtcggtaa tagccgcagt
     2341 ttaaaatttc accgtccgca atgccgccaa gcgcgtcggc taaagtcgag cgaaaaagtc
     2401 aagttttcct cccgcgatgc ggcgctggac agcggatacg cgccgtgccg gaattgcaag
     2461 ccgtgagcgt tctcgcctta gtcaaacgca ggggtgggcg ttccagaatt gccacaggag
     2521 accaagcagc gcaccgtgaa aactgtacta atcacagaca taggcaacag cgtagttggg
     2581 ggagagggta gggttttggg tgaaggtgcg caagcgttgg atgggggcga ggtatcccaa
     2641 ggcgctgtgc gcccgatgtt cgttgtaaca cttgaggtag ttgaccaccc ctctggggga
     2701 gaggtgggga tgtcccagag tgcggtagaa ttcttcgttg tcggtgcggt gggagtccgc
     2761 cagaggcgga ttgtagcgcg gggtacccgg agggatgagg gtatgctgaa tcgcagaggt
     2821 gcggagggtt tcttcgaagg gacggagagt cttgatgtga ccgaagaagc ggcaggtgag
     2881 gtcggcacca tggtcggtcg ggatggtctg gatgcggaaa ggatagaagc gtaccagtcc
     2941 gcctcaggcg gaaaggcgac cgcggtctgg ggcgtgagct cggggtaccg cgagacaaat
     3001 tgcaagcggg tgaacccacc gacggcggaa aactggtagg cgcgccgagg cattcccggg
     3061 cgcagggagc ggtatttgac atccaccggg actttctccc cggggaaggt caatccgcct
     3121 caggcggacg acgcgtgtat ctctttctcc ggggcttgcg agaatggagc tgtccggccc
     3181 gcttcaggat gttgtagatg ccggagattc gcctgaggcg aactgcttcg cgagaagaac
     3241 cagtgcaagc gtcgcggccc ataccccgtc tcgaggcgta accgcagtac cttgcgcgtc
     3301 cagtacttcc cgcatcgttt cggactgcgc ttcggcttgc gggagtgatt gtccagactc
     3361 tccggctgac cgccactcgc ctcccatcgg tggcgctact tgtaaaacgt cttggcggaa
     3421 atgccatacc tccgacatac ccgccgcgca ttgccgcact gcccgtactc acgaaaccat
     3481 tgcatccgcc ggtgccgatc agtatagtct gtactatcgg gcatgggtaa cctccgtgtt
     3541 ctttgaacgt cccagtccaa tt
//
LOCUS       DBNGZA010000132         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_236457, whole genome shotgun sequence.
ACCESSION   DBNGZA010000132 DBNGZA010000000
VERSION     DBNGZA010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_236457"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="AC16FU_02925"
     CDS             <1..1324
                     /locus_tag="AC16FU_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2993360.1"
                     /translation="EGKKSLPVSVLAGQPEIPQGLSAVGGRGEVTLFWAPNPEPDIDH
                     YNIYRSTAPNLPFGNIGSTTGAETTFVDQTVQNGVVYRYQISAVRVDGLASFLSPIVF
                     GVPMAFDQGLGLVDLTFNGFANLVNHDSVIAFYNRALRGYQFSLLNFSASIDATPSLA
                     NLSPYPVLLLASEDLRYPLPDSLFRVLSFYRIAGGKVLISGQANFSFYSYTDPNRYND
                     IFNLSGLPPQMLFAVDSAYAPRPAIYLAFTDSLKVQREFIGAQPKVQGYPLLEVDTAR
                     LNRYLCVPFVPPRCLAHNGTLPAVEYFFSADTSEDIYTFVSRYPDTSHAHGKAVAKRK
                     ITPGYSYVFFDLPLFFMKEAEAKAALKAGLADLGLTPTPYTVGDVNNNGAIDIADIAQ
                     LVQFVVFATNPPQPFEFVGDTNCDGLIDIVDIVLLLQYVLYGQPPLCL"
     gene            1498..3672
                     /locus_tag="AC16FU_02930"
     CDS             1498..3672
                     /locus_tag="AC16FU_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR2993361.1"
                     /translation="MTARRLFLTVGLGVAAGFLLIASDRPAGPMLVRAYFPDRQTFQK
                     IQALDLDIAEVNAQGLFCDIVASSSDLARLQGAGIRTEVTIRDLTLYYTNRTLATMGG
                     FHTFSEVVAALDSIHNEHPTITTAKDSIGTTIEGRTIWAMKISDNPGVDENEPEVFFN
                     GLTHAREPIGMEICLNTMRYLTNNYGSDPQVTSLVNDRELWFVPVVNPDGYVRNQTTN
                     PSGGGMWRKNRRNNGDGTFGVDLNRNYGYQWGYNNVGSSPNTSSDVYRGTAPFSEPET
                     QVIRNFCNSHNFTICLNYHSYGNLFLAPWGYTTEFPEDYDLLRAIGDSAVQSNGYISQ
                     PGWGLYFTNGDADDWQYGERSTKAKTISYTPEVGRNSDGFWPTLARIPVLISENLAPN
                     LFVARIADNPWRLLPPLPPTLTLGGTEVDTAGYVIHWSHSDPNNPAALFEVHELQSPY
                     LATDGAELGLARWRADGFTMTNARSFSGVASFYGGNANATVTTLTAKEGYLVSSVADT
                     LRFHTWYDTEVDYDYAYVEVSTDQGRTFTPIPGNITTDSNPNYLNRGNGINGSSGGLW
                     GEAKFPLGSYAGQRILIRFTYITDPFFTGEGFYVDDIDPVVTFASDNTLSSSVAAESL
                     AIGSRAPGVYYYEVRARDAQGQWGNFSVPDSLNVVGHPRFAKGDVNQDGTRDLTDIVM
                     LIQYVVFGDVIFADPSLADLDNDSVVDLTDIVALINIVVFGG"
     gene            complement(3721..>4622)
                     /locus_tag="AC16FU_02935"
     CDS             complement(3721..>4622)
                     /locus_tag="AC16FU_02935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR2993362.1"
                     /translation="IEQARAGHSFSQPRELRGPLAGREAIFMVTSAQIQDEADGLLGV
                     CVLGTDVTELRRLQEELVTKEKLAALGEMAAGLAHQLRNALAAISGFGKLLHRVAPEE
                     SQVKSVAAEILREAEATAALLDRFLSFAQPQKLAAEVVSAADLWQEVTAAKSVDLKER
                     NISCTVNLPPGLVIQGDLHLLKQVFVNLLQNAIEAVPQNGKIEITGVAGPEIVELAIS
                     DNGPGVPKALRTRIFDPFFTTKESGSGLGLSVARKVLELHGGTLTVRDQAGGGARFIL
                     TFPRQKATEDDAPLVLASSAPHQ"
BASE COUNT          925 a         1393 c         1267 g         1037 t
ORIGIN      
        1 tgaaggcaaa aagtcacttc cggtatcggt gctggcggga cagcccgaaa tcccgcaagg
       61 tttgtctgcc gtcggcggca ggggagaggt gacacttttc tgggcaccca atccagagcc
      121 ggatattgac cactacaata tctaccgatc cacggctcca aatcttccgt ttggaaacat
      181 tggttccact acgggagccg aaaccacttt cgttgatcaa acggtgcaga atggggtcgt
      241 ctaccgctat cagattagtg ccgtgcgggt cgatggactg gcaagtttct tgtccccgat
      301 tgtgttcggc gtgccgatgg cttttgacca gggtttgggg ttggtcgatt tgacttttaa
      361 tggcttcgcg aatctggtaa atcatgacag cgttattgcc ttctataacc gcgcgctgcg
      421 ggggtatcaa ttttcactcc tgaatttctc tgcttccatc gacgcgacgc catctctggc
      481 gaacctctcg ccgtaccccg tcctcctcct tgcgtctgaa gatttgcgct accccctacc
      541 cgactcgctc ttccgtgttc tttcgttcta tcgtattgct ggtggaaaag ttctgatcag
      601 cgggcaggcg aacttctcat tctacagtta cactgatccc aaccgctaca acgatatttt
      661 caatctctcg ggtctgccgc cacagatgct ttttgctgta gatagtgcct acgctccacg
      721 ccctgccatc taccttgcct tcaccgattc attaaaagtt cagcgagaat tcatcggggc
      781 gcagcccaaa gtgcagggct atccgctact ggaagtggat acggccaggc tcaaccgcta
      841 cctgtgtgta ccgtttgttc cgccccggtg ccttgcccac aacggcacct tgccggccgt
      901 ggagtatttc ttcagcgctg atacctcaga ggacatttac acttttgtct cgcgctaccc
      961 cgacacttcg cacgcccacg gcaaagcggt agccaagcgc aaaattacac cggggtattc
     1021 ctacgtcttc ttcgacctcc cgcttttctt catgaaagaa gcagaggcga aagccgccct
     1081 aaaggccggg ctggcagatc tgggtttgac gcctactccc tacaccgtcg gtgacgtgaa
     1141 caacaacggg gcaatcgaca ttgccgacat agcgcagttg gtgcagtttg tcgtctttgc
     1201 caccaacccg ccccaaccgt tcgagttcgt cggcgatacg aactgcgacg gattaatcga
     1261 cattgtcgat atcgttcttc tgctgcagta cgtcttgtac ggccagccgc ctttgtgtct
     1321 gtaacctccc accgagggtt gcccgcaact cttcctgccg cccgctgcag ggggggccag
     1381 accagcgaaa atttttcttg catggggtct aaaaactgtt atattctccc cgcgcggcga
     1441 agccagaaag aggtcgcgct ttcacgtact ggctgacctg atgcggagcg aaatgtgatg
     1501 acagcgcgaa gacttttcct gaccgtcggg ctgggtgtgg ccgctggttt tctccttatt
     1561 gccagtgacc ggcccgcggg cccgatgctg gtgcgggcct actttcccga ccggcaaact
     1621 ttccagaaaa tccaggcgct tgatttagac atagccgagg tcaatgccca gggtttgttc
     1681 tgcgacattg ttgcttcgtc atccgacctg gctcgcctcc agggagccgg cattcgtacg
     1741 gaagtgacca tccgcgatct caccctctat tacacgaacc gaacgcttgc caccatgggt
     1801 gggttccata cgttcagtga agtggtggcc gcgctggatt caatccacaa cgaacacccc
     1861 accatcacca ccgccaagga cagcatcggg acgaccatag aaggacgcac catctgggcg
     1921 atgaaaattt ccgataaccc cggcgttgac gaaaatgaac cggaagtctt tttcaacggg
     1981 ctcacccacg ctcgcgaacc gatcgggatg gaaatctgtc tgaataccat gcgttacctg
     2041 accaacaact acggcagtga cccacaggtg acgtcactcg tgaacgaccg ggagctctgg
     2101 ttcgtgccgg tggtcaaccc cgacggctac gtgcgcaacc agacgactaa cccctcaggc
     2161 ggcgggatgt ggcgcaagaa caggcgcaac aacggggacg gcacgttcgg cgtcgatttg
     2221 aaccggaact acggctacca gtggggctac aacaacgtcg gctccagtcc taacacttcc
     2281 agcgacgtct atcgcggtac ggcccctttt tccgagccgg agactcaggt gatccgcaac
     2341 ttctgcaaca gccacaattt caccatctgc ctcaactacc attcctacgg gaatctcttc
     2401 ctggccccct ggggttacac caccgagttt ccagaagact atgatctcct gagggcaatc
     2461 ggggacagcg ccgtgcagtc caacgggtac atcagccagc cgggctgggg gctgtacttc
     2521 accaacggcg acgctgacga ctggcagtat ggggaaagaa gcacgaaagc caagaccatt
     2581 agttataccc cggaagtagg gcggaatagc gacggttttt ggccgactct ggcccgcatt
     2641 ccggttttaa tcagtgagaa tctcgccccg aatctcttcg tggcccgcat tgcggataac
     2701 ccctggcggc tcttgccccc cttgccgccg actctaacac tcgggggtac ggaggttgac
     2761 actgcgggct atgtcattca ctggagccac agtgatccca ataacccagc ggcactgttt
     2821 gaagtccatg aactgcaaag tccctatctg gcgaccgatg gcgcggaatt ggggctggcc
     2881 cgctggcggg ctgacggctt tacgatgacc aacgcccgca gtttttccgg ggtcgccagc
     2941 ttctacggcg ggaatgcgaa cgccaccgtc accactttga cggcgaagga agggtacctt
     3001 gtgtccagcg tggccgatac gctgcgcttc cacacctggt acgacactga ggtcgactac
     3061 gactacgcct acgtcgaggt ctccaccgat cagggacgga ccttcacccc cattccgggc
     3121 aacatcacga cggattcaaa tccgaattat ttgaaccggg gcaacggcat caacggttcc
     3181 tcgggcggcc tgtgggggga ggccaaattc cctctgggga gttacgccgg tcagagaatt
     3241 ctcatccgct tcacctacat caccgatccc tttttcaccg gggaaggttt ctacgtcgat
     3301 gacatcgatc cggtggtcac ctttgcgtcg gataatactc tttccagttc ggtggcggcc
     3361 gaatcgctcg ccatcgggtc gcgtgctccg ggagtgtact actatgaggt gcgggcacgg
     3421 gatgctcagg ggcagtgggg aaatttcagc gtgcctgaca gtctgaacgt ggtcggtcat
     3481 ccccgctttg ccaaagggga cgtcaatcag gatgggacgc gcgaccttac cgacatcgtc
     3541 atgctcattc aatacgtcgt ctttggggac gtcatcttcg cggatccgtc actggccgac
     3601 ctggacaatg acagcgtggt cgatctaact gatattgtcg ctctgattaa tatcgttgtg
     3661 ttcggcgggt agcccggtgg cgctgccgag cttgcgatcc cgctcagcgg gcgggatttt
     3721 ttattggtgc ggtgccgagg atgccaggac gagtggagca tcatcttccg tcgctttttg
     3781 ccgggggaaa gtgaggatga aacgagctcc gccgccggcc tggtcgcgca cggtcagcgt
     3841 tcccccgtgc aattccagca ccttgcgggc cacgctcagg cccagaccgc taccgctttc
     3901 cttggtggtg aaaaacggat caaaaatgcg ggtccgcagg gctttcggga cgcccggtcc
     3961 attgtcactt atggcaagtt ccacaatttc gggcccggcg acgccggtga tctctatttt
     4021 cccattctgc ggaacggctt cgatcgcatt ctgcaacagg ttcacaaaga cctgcttcag
     4081 caggtgaagg tcgccctgga tcaccagccc gggcggaagg ttcacggtac agctgatatt
     4141 tctctccttc aagtcgacgc ttttcgccgc agtgacctcc tgccagaggt cggccgccga
     4201 caccacctct gcggccagtt tttgcggctg cgcaaaggag aggaaccggt cgaggagcgc
     4261 ggcggtcgct tcggcttccc ggagaatctc cgcggcaacc gattttacct gcgactcttc
     4321 gggagcgact cgatgcaaaa gttttccgaa cccgcttatt gccgcgaggg cgttgcgcag
     4381 ctggtgtgcc agaccggccg ccatttctcc cagtgcggct aatttctctt tggtgaccag
     4441 ctcctcctgc agacggcgta attccgtgac gtcggttccc agaacgcaga ctcctaacag
     4501 gccgtccgct tcgtcctgaa tctgagcact ggttaccata aaaatcgctt cgcgtccggc
     4561 caagggaccc cgcaactccc ggggttggga aaacgagtgt ccggcccgcg cttgttcaat
     4621 ga
//
LOCUS       DBNGZA010000133         6148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2252104, whole genome shotgun sequence.
ACCESSION   DBNGZA010000133 DBNGZA010000000
VERSION     DBNGZA010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2252104"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16FU_02940"
     CDS             complement(<1..418)
                     /locus_tag="AC16FU_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993363.1"
                     /translation="MKTAQVAMLLGAVACSLAFTPATLKADCKPADYYTRSAKVNYIQ
                     IRNPDYKKALALLNEGRACYPKNAEIYFWLGNLYGELDSIKLMSAFYDTSVSLSDKYK
                     KEITNTRVSKWGDLFQTGLNALKADTVQSDSLKLATR"
     gene            complement(447..1844)
                     /locus_tag="AC16FU_02945"
     CDS             complement(447..1844)
                     /locus_tag="AC16FU_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR2993364.1"
                     /translation="MSLLNRENGRRRMVIRQSAFRFVVLALFTLTADHTSAQKEVSLT
                     IYNQDLGLVKEIRTLDFARGRNRIQFTDVASAIDPTSVRFRALEHPESINLLEQNYQF
                     DLVNSEKILSRYVDKSIQAIAKDGKVFEGTLLSVSPGSLVLQEPSGGLRIVSRDNILD
                     IAFPALPEGLFTRPTLLWLIDAETAGKRKAEVSYLTSAINWHAEYVATVDATDTNLEL
                     AGWVSIDNRSGAGYADAKVKLIAGEIHRAEAPRPRYAEGLAMEQMAKAPAFAEEAFFE
                     YHLYTLQRPATVANNEIKQISLFEPAQTPVKKIFTFEGQRRAKVNVTLEFVNAQARGL
                     GMPLPAGKIRVMKKSADGGMEFVGEDMIDHTPKDEKVRIEVGSAFDLVGERKVKDRRQ
                     ISDRVWEEDYEISLRNRKAENVEIVVTEHLYGYWEIRNASSPYTKKDANTVEFPVKVP
                     AGAEVVLTYTARFRS"
     gene            complement(1912..4638)
                     /locus_tag="AC16FU_02950"
     CDS             complement(1912..4638)
                     /locus_tag="AC16FU_02950"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR2993365.1"
                     /translation="MAKTENTERPQPAAPPLEERTARESLPKVYDPKLVEEKWYRQWL
                     EYGLFHGNPKTKKPVYSVVIPPPNVTAALHIGHALNNTLQDVLIRWKKLQGFETEWLP
                     GIDHAGIATQVMVEKELAKEGKTRQQLGREAFLEKIWDWKNKYGDTILNQIQKVGCAC
                     DWQRTRFTLDEGLSRAVSDVFVRLYEKGLIYRGNYIINWCVRCQTAISDDETERTEIN
                     SNLWYLRYPLEDKSGHVVIATTRPETMLGDTAVAVNPDDSRYAHLVGKTAILPLLGRR
                     LPIIADAYVDPEFGTGALKVTPGHDRNDFEIGQRHKLEVVNILNLDGTLNQNAGKFAG
                     QDRLKARAAVVEALETEGLLEKIEPYNLSAVTCSRCHSIIEPLISLQWFVKMQPLAEP
                     AARAVREGQIHFHPEHWAKVYLHWMDTIHDWCISRQLWWGHRIPVWYCRDCGEVNVAR
                     TTPAVCRKCQSKNLKQDEDVLDTWFSSWLWPFSTFGWPDETPELKKFYPTKALFTAPE
                     IIFLWVARMVMAGLEFTGKIPFADVYLHGTVRDAIGRAMSKSLGNGIDPLDIIKDHGA
                     DALRVSLILAQPDGQDPWISPNTFELGRNFANKLWNATRFALLNLEDFDRSPAEIKDP
                     AMLSLPDRWIFSRLHQTIQTMTAQLDDFRYNAAAKTLYDFAWGDFCDWYLELSKVLLK
                     NAASGAKRKTQAVLFTTLNDLLLLLHPFLPFLSEELWHKLNGIPENDSFRSILQEHWP
                     QANAKLINAGLEKEVAWLQSLITAVRALRGELDIPPVKKSRLVVSVDDLAQKESLEFQ
                     LDFVRELARAEAVEVGQHLRRPHQSAVAVLSGAEVYLPLEGLVDIAAEKSRLSRAKEK
                     YEHLLSAVERKLATPEFAQKAPPEVVQKEETKRADFAKAVAKLQALVEQLEK"
     gene            4809..5597
                     /gene="mazG"
                     /locus_tag="AC16FU_02955"
     CDS             4809..5597
                     /gene="mazG"
                     /locus_tag="AC16FU_02955"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR2993366.1"
                     /translation="MKRKKAQQPFDELVQIMARLRGPGGCPWDRKQNHKTLLPYLVEE
                     CYEVLEAIESRHYGKLKEELGDLLLQVVFHAQLASERGRFSVYESVADLNRKLISRHP
                     HVFGRTQRISARKVLHNWERLKLTDPSGNHRSVLDGIPRTLPALLFAFRTQEKVARFG
                     FEWKQISDVKDKIAEELTELKRAQRKRRKTAVEEELGDLFFALVTLARKSGIDPEAAL
                     RRTVRKFQRRFAGMERELQRQGKKLGTVPLRELEHLWGEAKAKR"
     gene            5925..>6148
                     /locus_tag="AC16FU_02960"
     CDS             5925..>6148
                     /locus_tag="AC16FU_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2993367.1"
                     /translation="MSRVLQKLLGSSQGWFAPLAGIILVTFFISLPSASGQSTLLHQK
                     DGDAASDFLGNSVAGAGDVNGDGKADFIIGA"
BASE COUNT         1258 a         1637 c         1846 g         1407 t
ORIGIN      
        1 ggcgggtagc gagtttgaga ctgtcgctct gtaccgtgtc ggccttgagg gcgttcaatc
       61 ccgtctggaa cagatcgccc cacttgctga cccgggtatt ggtaatttcc ttcttatact
      121 tgtccgaaag ggagacggaa gtgtcgtaga aggcgctcat caacttgatc gagtccagtt
      181 caccgtagag gttgcccagc cagaagtaga tttcggcgtt tttgggatag caggcgcggc
      241 cctcattcaa aagcgccagc gccttcttgt agtccgggtt tctgatctgg atgtagttca
      301 ccttggccga gcgggtgtaa tagtcggcgg gcttgcagtc ggccttaagt gtggcgggtg
      361 tgaaagccaa agaacaggct actgccccta acagcatggc aacttgtgcg gttttcattg
      421 ccctcccttt cgttactcta tccatttcaa ctccggaagc gggcggtgta ggtaagcacc
      481 acctcggcac cggctggcac ttttacgggg aattcgaccg tgttggcgtc ttttttcgtg
      541 tagggagaac tggcattcct gatctcccag tagccgtaca ggtgctcggt cactactatt
      601 tctacgtttt ccgccttgcg gttgcgcagg gaaatctcgt agtcctcctc ccagacccgg
      661 tcggaaatct gccgccggtc tttcaccttc cgctccccca ccaggtcgaa tgcggagccg
      721 acttcaatcc ggactttctc gtctttgggg gtgtggtcga tcatatcttc gcccacgaat
      781 tccattcccc cgtcggcgga ctttttcatc acccggattt tgcccgccgg caggggcatt
      841 cccagccctc tggcctgggc gttgacgaat tccagcgtca catttacttt cgcgcgcctt
      901 tgaccctcaa aggtgaagat tttcttgaca ggggtctggg ccggctcaaa cagggagatc
      961 tgttttatct cattattggc gacggttgca ggccgctgga gggtatacag gtggtattca
     1021 aagaaggcct cttcggcaaa ggccggggcc ttagccattt gctccattgc cagcccttcg
     1081 gcatagcggg ggcgcggcgc ctcggcccgg tgaatttcac cggcgatcag tttgactttg
     1141 gcgtcggcgt aaccggcccc ggaacgattg tcgatcgaca cccagccggc caattcgagg
     1201 ttggtgtcgg tggcatccac cgtggccacg tactcggcgt gccagttgat ggcggaggtc
     1261 aggtagctca cttcggcctt gcgcttccct gctgtttccg cgtcgatcag ccagagcagc
     1321 gtcggccggg tgaagagccc ttccggcagg gcggggaagg caatgtccag gatgttgtcc
     1381 cggctcacta tgcgcagacc gccgctgggt tcctgcagga caagactccc cggcgaaacc
     1441 gaaagcaaag ttccctcgaa gaccttgcca tctttggcga tggcttgaat tgacttatca
     1501 acatagcggg agaggatttt ttcggaattg accaggtcga actggtaatt ttgttccaac
     1561 aggttgatgc tttccggatg ctccagcgcc cgaaagcgga ccgaggtcgg gtcgatggcg
     1621 gaggccacgt cggtgaactg gatgcgattc cttccgcggg caaaatccag cgtcctgatt
     1681 tctttcacga gtcctaagtc ctgattgtag atcgtgagac tcacttcttt ttgcgccgaa
     1741 gtatggtcgg cggtcagggt gaagagagcc agcaccacga atcgaaacgc actttgccgg
     1801 atcaccattc tcctgcggcc gttttcccgg ttcaaaagcg acatgcgctc ctccttttct
     1861 ggtcaaccgg gataccggcg gagccgggaa atattcccga acatgtcgac atcacttttc
     1921 caactgttcc acaagggcct ggagtttggc caccgctttg gcgaagtcag cccgtttggt
     1981 ctcttctttt tgcactacct ccggcggggc cttctgtgcg aattcgggcg ttgcaagttt
     2041 tcgttccacc gccgatagca ggtgttcgta cttctccttt gcccgggaga ggcggctttt
     2101 ttcggcggcg atgtccacca gtccttccag aggcaggtaa acctccgctc cggagagaac
     2161 cgccaccgcc gattgatgcg ggcgtctcaa gtgttgcccc acctctaccg cttcggcacg
     2221 agccagttcg cgcacgaaat cgagttggaa ttcgagggat tccttttgtg ccagatcgtc
     2281 cacactgacc accagccggc tcttcttaac gggcgggatg tccaattcgc cgcggagggc
     2341 gcgcacagcg gtgatgagcg actggagcca ggcgacctct ttttccagtc cagcattgat
     2401 gagtttggcg ttcgcctgcg gccaatgctc ctggagaatt gagcggaagg agtcgttctc
     2461 cgggatgccg ttcagtttgt gccacaattc ctcgctcaga aatggcagaa agggatggag
     2521 caaaagcaag aggtcgttca gcgtggtaaa gagcaccgcc tgcgtctttc gtttggcgcc
     2581 ggaggcggca tttttcaaga gcactttgga cagttccaag taccagtcgc agaagtcgcc
     2641 ccaggcaaaa tcgtagaggg tcttggcggc ggcgttatag cggaagtcat ccagctgggc
     2701 ggtcatcgtc tggatggttt gatgcaagcg gctaaaaatc cagcggtctg ggagactcaa
     2761 catagccgga tcctttatct ccgccgggga gcgatcgaaa tcttccagat tgagcagggc
     2821 gaagcgggtg gcgttccaga gcttgttggc gaaattgcgg cccagttcaa aggtattcgg
     2881 cgaaatccag gggtcctggc cgtcgggctg ggccaaaatc aaactgactc gcagggcatc
     2941 ggcgccgtga tctttgatga tgtctaaagg atcgatgccg ttccccagcg acttggacat
     3001 ggcccggccg atggcgtcgc gtaccgtgcc gtggaggtaa acgtcggcaa agggaatttt
     3061 gccggtgaac tccagcccgg ccatcaccat acgggccacc cagaggaaga tgatttccgg
     3121 cgcggtgaaa agcgctttgg tcgggtagaa ttttttcaac tccggcgttt catccggcca
     3181 gccgaaggtc gaaaaaggcc agagccagga agagaaccag gtgtcgagga cgtcttcgtc
     3241 ctgcttaaga ttcttgctct ggcatttccg gcagacggcc ggtgtcgtcc gtgcaacatt
     3301 cacctcgccg cagtcccggc agtaccagac cgggatgcgg tggccccacc agagttggcg
     3361 ggaaatgcac cagtcgtgaa tggtatccat ccagtggagg taaactttgg cccagtgctc
     3421 cgggtggaag tggatttgcc cctcgcgcac cgcccgggcg gccggctcgg ccaaaggctg
     3481 catcttgacg aaccactgga gggaaatcaa gggttcgata atcgagtggc agcgggaaca
     3541 ggtaaccgcc gatagattgt agggttcgat tttttccaaa agcccttcgg tctctaacgc
     3601 ttccaccacg gcggcgcggg ctttgagacg atcctggccg gcaaacttgc cggcgttctg
     3661 attcaacgtc ccgtccaaat tgaggatatt caccacttcc aatttgtgcc gctggccgat
     3721 ttcaaaatca ttgcggtcgt ggccgggagt caccttgagc gcgccggtgc cgaattcggg
     3781 atcgacgtag gcatcggcaa tgatcggcag gcggcggccg agcaaaggca aaatcgccgt
     3841 cttacctacg agatgggcgt agcgggaatc gtccggattc accgccacgg cggtatcacc
     3901 cagcatcgtt tcgggccggg tggtggcaat gacgacgtgg ccgcttttat cctccagcgg
     3961 gtagcggagg taccagaggt tcgaattgat ctcggtgcgc tcggtttcgt cgtcggaaat
     4021 ggcggtctgg cagcggacgc accagttgat gatgtagttg ccccggtaaa tcaacccttt
     4081 ctcatagagg cggacaaaaa catcgctcac cgcccgcgat agtccctcgt ccagcgtaaa
     4141 acgggtgcgc tgccagtcgc aggcgcagcc gactttttga atctgattaa gaatcgtatc
     4201 gccgtacttg tttttccagt cccagatttt ttccaggaat gcttcgcgac cgagttgctg
     4261 gcgcgttttc ccctctttgg ccagttcttt ttccaccatc acctgggtgg cgatgccggc
     4321 gtggtcgatc ccggggagcc actcggtctc aaacccctgc agtttcttcc agcggatgag
     4381 cacgtcctgc agggtgttgt tcaaggcgtg gccgatgtgg agcgcggcgg tgacgttggg
     4441 cggcgggatg acgaccgagt agaccggttt tttcgttttg ggattcccgt gaaaaaggcc
     4501 gtactccagc cactgccggt accacttctc ctcgacgagt tttgggtcat aaactttggg
     4561 cagcgattcg cgagcggttc gctcctccaa aggcggtgcc gccggttgcg gtcgttcggt
     4621 attctccgtt tttgccatta cttttgatga tagggcagat aatgagcttt gtcaagaatg
     4681 cgttgtttga atgcgggtga actggttata ctggtaacct gtcatcggat ttcgaacgga
     4741 tttaaaacgg attagaaaac ggtgtggctt tttttgattg agtggtgcta catgaaggaa
     4801 atttcttgat gaaaaggaag aaagcgcaac agccatttga tgagttagta caaataatgg
     4861 cccgcctgcg ggggccgggc gggtgcccct gggaccggaa gcagaaccat aagacgcttt
     4921 tgccgtattt agtcgaagag tgctacgagg tgttagaggc catcgaatcc cgccactacg
     4981 ggaaactcaa agaggaattg ggtgacctcc tgctccaggt ggtgttccac gcgcaattgg
     5041 cgtcggagcg gggacgattt tcggtttacg agagcgtcgc cgacctgaat cggaagttaa
     5101 tcagccgcca cccgcacgtc ttcggtagaa cccagcgcat ttccgcccgg aaagtcctgc
     5161 acaactggga acgactgaag ttaaccgatc cctcaggcaa tcaccgctcg gttttggacg
     5221 gcattcctcg aacgctcccg gcactgctct tcgccttccg cactcaggag aaggtggcgc
     5281 ggttcggctt tgaatggaag cagatctccg acgttaagga caagattgca gaggaactga
     5341 ccgaactgaa acgggcgcag aggaagagga gaaaaacggc ggtggaggag gaattgggcg
     5401 accttttctt tgctctggtt acgctggcgc ggaaatcggg gattgacccc gaagcggcgc
     5461 tgcggaggac ggtgcggaag tttcaaaggc gctttgccgg aatggagcgg gagttacaaa
     5521 gacaggggaa gaaattgggg acggtgccgc tgagagaact ggaacatctc tggggggaag
     5581 caaaggcaaa gagataaggt ggcctgatcc gcaggagagc aaccaaaacg gggggctagg
     5641 acaaggggtc aggaacggcg aacctgtcgt cgcgggaaga aggacaagtc cgcagcttta
     5701 gttaaccact tgagggacag ataattaggg gcgggacttc ttgtgagtgt ggccaggatc
     5761 acctaccggg cgggaaaaat attgataaaa gggtaaaaat gtccttgaca agagaaaacc
     5821 ggttaagtat tctatccttg cacggacacc tcgaagtcgc gtgacggata agcaatgaag
     5881 acttaggtca acttcccaac cctctttccc aagggaggtg tagtatgtcg agggtacttc
     5941 aaaaactttt ggggtcttct caagggtggt tcgcaccgct cgcgggcatc attcttgtca
     6001 cttttttcat ttcattaccg tccgcctccg gccaatccac cttgctccat cagaaagacg
     6061 gcgacgccgc aagtgacttt ctgggtaact ccgtcgccgg agcgggggat gtgaacgggg
     6121 atggaaaggc cgatttcatt atcggcgc
//
LOCUS       DBNGZA010000134         4488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2039590, whole genome shotgun sequence.
ACCESSION   DBNGZA010000134 DBNGZA010000000
VERSION     DBNGZA010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2039590"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1048..2643)
                     /locus_tag="AC16FU_02965"
     CDS             complement(1048..2643)
                     /locus_tag="AC16FU_02965"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR2993368.1"
                     /translation="MPEPTGTDNRPVLSKEIIINVSEHETRIAILEEGKLVELLVERA
                     EAERMVGNIYKGKVTNVLPGMQAAFIDIGAEKAAFLHASDAGEVVTGDEESEESADEA
                     EKPQRGRGDSDREDERRFRSIQEVVAKGQEILVQIVKEPISTKGPRVTTEISLPGRFI
                     VLVPAWKQVRVSKRIEGWPERRRLRRIVTELLPPGLGAIVRTEGEGKTEREFKADIKL
                     LVHTWKKILRQAARKGAPALIHKEFGITSSIIRDLFTRDVDRLVIDSRKEYKLILGYL
                     RSVAPELREKVSLYAESVPIFDLYGIESEIEKMLDRKIWIKRGAYIVIDQTEAVVAID
                     VNTGRFVGKGNQEQTIFRTNLEAAKEVARQIRLRDIGGLIIIDFIDMYSRENRRRLFE
                     EFRAGFKNDRSKFSISPVSEFGLIEMTRERVRPSLMYTFSEPCPTCSGFGRVLSRETV
                     ATKIERWFKRAKAGAKYKRFKLIISPYLAEAIVDANPSRVRQVAQEFKVEIDVIRDTT
                     MPPEEFRVIAAQDGKDITELFSA"
     gene            complement(2727..3656)
                     /gene="fmt"
                     /locus_tag="AC16FU_02970"
     CDS             complement(2727..3656)
                     /gene="fmt"
                     /locus_tag="AC16FU_02970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR2993369.1"
                     /translation="MRSVFLGASEFALPSLQKLARSHHTVAAVVTHPDRPQGRGLKPE
                     PTPVKRQAQELGLPVFTPAKLDAPDFLEQMRGLQSDLMVVVAFRFLPEALYAIPPKGA
                     INLHASLLPKYRGAAPIQWVLIHGAKVTGLSTFFLNRSLDQGDLLLQREVAIAPDDNF
                     GSLSARMAEQGAGLLLETIDRIEAGTLKPKKQDNSQATRAPKITKEMCVIDWSRSAEK
                     IHNLVRGLAPEPGAVTFFQGKRLKILRTGITPEKETGSAGAILHADPKQGLSVAAGEG
                     SLELLEVQPEGKSPVSGAEFVRGYRPRTGERLG"
     gene            complement(3657..4172)
                     /gene="def"
                     /locus_tag="AC16FU_02975"
     CDS             complement(3657..4172)
                     /gene="def"
                     /locus_tag="AC16FU_02975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR2993370.1"
                     /translation="MALLTIRQYGDPVLRQTAEEVTNINGTLARLAADMLATMKSAKG
                     VGLAANQVGSLARLYVVDTSPFLLNGGTLVLINPRVVETKGEETAEEGCLSIPGIYED
                     IIRPQSATVEGLDLDGKTVTYEGKGMVARVFLHELDHLNGVLFIDHLSPAKRELLKSR
                     LREIKEKHWAL"
     gene            complement(4179..>4488)
                     /gene="yajC"
                     /locus_tag="AC16FU_02980"
     CDS             complement(4179..>4488)
                     /gene="yajC"
                     /locus_tag="AC16FU_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR2993371.1"
                     /translation="GMGPQGGGGGGGGGMMALLYFGLLILIFYFFFIRPQQKKHKTQQ
                     KMLSELKKGDRVITNGGMFGTIVGIDEKENMIVLKVGENTKLEFLRSAIAGRVEKGSE
                     "
BASE COUNT          832 a         1398 c         1250 g         1008 t
ORIGIN      
        1 actggtgccc cttcccccgc taattggggg ttgccttgga ggtaaaacgg atgccctctt
       61 cctttcgcgt aggcgggact tttagtcccg aaaaacggga cggcactgaa agtgccgtct
      121 acgcggagag agaaaatttg gggtcgtctg gggggtaaaa cggacaaagt gcgaaatagt
      181 agagggcctg agtgctctca ccggcctctg tcggtagacc cgtaccgggt gagctggcca
      241 acttcaggtc gaagatcccg tccaaaggcg ggaccgggta cccgttaaac aaagagcttc
      301 aagcgccagc gcacgccggt cggtccgacc cgcctcaggc ggataggatc gggcatcact
      361 ctcgccagga gaaaaaggcc acgtggccac cttggaaaac ccaggggaag agaaggaata
      421 gaaagacaca ggcagatgag ataacccgct taatccgctg gctaatccgt tgtcagttct
      481 ccgtgccccc cgtgtctccg tggtgagagc tttttatctg ctccatctgc tgtgaatgcc
      541 gttgcttttc ctcaaatcct aactcccatc tcccgaatcc atttttttcc taccacctat
      601 caccttccac ctaccacctt gctttgttct tactcctgct ccaccgacac cccgacacct
      661 tgacacttcg acacccttgg gccgccctct gtgacattaa ccccaagacc gctttacgct
      721 tgcgcaactg ccgctggtac gtcgcccctg acgatgagtg aatttccgtg ccgctccccc
      781 gaaccgtgat tattctcttg gacgataaga ccttgctccg cccgattgcg ctttttatcg
      841 tgaaaaccgg tttacttccc cgaactgccg ctgccccttg cccggtagag agttgcgcag
      901 ttttcccgcc aaacggcttt tccccgcttt tctgcggatc tcgttgccgt tcaaccagga
      961 cgcaaaagaa aaaatttatg ccgttttgcc cccctcccag gaactttggt tccgccttaa
     1021 cctcgacacc ccgacacgga gttcgcttta tgccgagaaa agctccgtaa tgtccttgcc
     1081 gtcttgcgcc gcaatcaccc ggaactcctc cggcggcatc gtggtgtcgc ggatcacgtc
     1141 gatttccact ttgaactcct gcgccacctg ccgcacccgg ctggggttgg catccacgat
     1201 ggcctcggcc agataagggg agatgatcag tttgaagcgt ttgtatttcg ctcccgcctt
     1261 ggcccgcttg aaccagcgct cgattttagt cgccactgtc tcccgcgaga gcacccggcc
     1321 aaagccggaa caggtggggc agggctcgga gaaggtgtac atcaaagagg gacgcacccg
     1381 ctcgcgggtc atctcgatca agccgaattc cgagacgggc gatatggaaa acttggagcg
     1441 gtcgtttttg aagccggcgc ggaactcttc gaagagccgc cgccggttct cccgcgagta
     1501 catgtcgatg aagtcgatga tgatgagccc gccgatgtcg cgcagccgga tctggcgcgc
     1561 cacctctttg gccgcttcca ggttggtgcg gaaaatcgtt tgctcctggt tccccttgcc
     1621 gacgaagcgg ccggtgttga cgtcgatcgc caccaccgcc tcggtctggt cgatgacgat
     1681 gtaggccccg cgcttgatcc agatcttgcg gtcgagcatt ttttcgattt ccgactcgat
     1741 gccgtagaga tcgaagatcg gcaccgattc ggcgtagagg gaaactttct cgcgcagctc
     1801 cggcgccacg ctccgcaggt agcctaagat caacttgtac tcttttcggg aatcgatgac
     1861 taaccgatcc acgtcgcgag tgaagaggtc gcggatgatg ctggaggtga tgccgaactc
     1921 tttgtgaatc aaagcgggcg ctcccttgcg cgccgcctgg cgcagaattt tcttccaggt
     1981 gtggactaag agcttgatgt cggctttgaa ctcccgttcg gtcttcccct cgccttcggt
     2041 gcggacaatg gcgcccaggc ccggcggcag aagttcggtc acgatgcgcc gcagccttcg
     2101 cctttccggc cagccctcaa tccttttgga gacccgtacc tgcttccagg cgggcacgag
     2161 cacgatgaag cggccgggga gcgaaatttc ggtggtgacc cgcggccctt tggtgctgat
     2221 cggctctttc acgatctgca ccaaaatttc ctgacccttc gccaccacct cctgaatgct
     2281 ccggaagcgg cgctcgtctt cccggtcgga gtcaccccgg ccgcgctgcg gtttttcggc
     2341 ttcgtcggcc gattcctcgg actcctcgtc gccggtcacc acttcgcccg cgtcgctcgc
     2401 gtgcaaaaag gcggcttttt cggcgccgat gtcgatgaaa gctgcctgca ttcccgggag
     2461 gacgttcgtg actttccctt tgtaaatgtt ccccaccatc cgctccgctt cggcccgttc
     2521 gaccaaaagt tcgaccaact tgccttcttc caggatggca atgcgggtct cgtgctcgga
     2581 gacgttgatg atgatttctt tgctcaaaac ggggcggttg tctgttccgg ttggttcagg
     2641 cataggtgtt ttcacgtggg acgcgggcgc cggcgggcgt gccggtggct gacccgcgtc
     2701 tttagttctg acggtttcaa ctctgttcat cccaatcttt cccctgttcg gggacggtag
     2761 ccgcgcacga attccgcgcc ggaaacaggt gacttccctt ccggctgcac ctccaagagt
     2821 tccagacttc cctctccggc agcgacggag agaccctgct tcggatcggc gtggagaatg
     2881 gcaccggctg aaccggtttc tttttctggc gtgatgcctg tccgcaaaat cttcagccgt
     2941 ttgccttgga aaaaggtgac ggcgcccggc tcgggcgcca aaccccgcac cagattgtga
     3001 atcttttcgg cggaacgcga ccagtcaatg acgcacattt ctttggtaat cttcggcgcc
     3061 cgggtggctt gtgaattgtc ctgttttttc ggtttgaggg tacctgcttc aatacggtca
     3121 atggtctcca agagaagtcc ggcgccttgc tcggccatgc gcgctgaaag actcccgaag
     3181 ttgtcgtccg gcgcaatcgc cacctcccgc tggagcaaaa ggtcgccttg atcgagactg
     3241 cggttaagga aaaaagtcga gagaccggtc acctttgctc cgtgaatgag cacccactga
     3301 atcggcgctg ccccgcggta cttgggcaga agcgacgcgt ggagattgat ggcgcccttc
     3361 ggcggaatgg catagagtgc ttccggcagg aagcgaaagg cgacgactac cataaggtca
     3421 ctctgcaaac cgcgcatctg ttccagaaaa tcgggcgcat ccagtttggc cggtgtgaac
     3481 acgggcaacc ctagttcctg cgcttggcgc ttgaccggcg tcggctccgg cttcaaaccc
     3541 cggccctgtg gccggtcggg gtgggtgacg accgccgcca cggtatggtg ggagcgggca
     3601 agcttttgca aactgggaag cgcaaactcc gacgccccca ggaagactga gcgcatctac
     3661 aacgcccagt gtttctcttt gatctcccgc aggcggctct tgaggagctc acgcttggcc
     3721 gggctcaggt ggtcgatgaa caacaccccg ttcaagtggt ccagttcatg caaaaagacc
     3781 cgcgccacca tcccctttcc ctcgtaggtt acggttttgc cgtcgagatc gagtccctcc
     3841 accgtcgcgg attgggggcg gatgatgtct tcgtagatgc cgggaatcga aagacaccct
     3901 tcttcggcgg tctcttcgcc tttcgtctcc accactcgcg gattgatgag caccagcgtt
     3961 ccgccgttca aaaggaaagg cgacgtatcc accacgtaca ggcgcgccag ggagccgacc
     4021 tgattggccg ccagccccac gcctttggca gatttcatgg ttgccagcat atccgcagcc
     4081 agtcgcgcca aagtgccgtt gatgttggtt acctcctcgg cggtctgccg cagcaccgga
     4141 tcgccgtact ggcggatggt caaaagcgcc acgccggcct attcgctccc tttctccacc
     4201 cgtcccgcaa tggcggagcg gaggaattcg agcttcgtat tctcacccac tttcaaaacg
     4261 atcatgtttt ctttttcgtc gatgccgacg atggtgccga acatgccgcc gttggtgatc
     4321 acccggtcgc cttttttgag ttcggagagc atcttctgct gagtcttgtg cttcttctgc
     4381 tggggccgga tgaagaagaa gtagaagatc aaaattaaaa gcccgaagta gagaagcgcc
     4441 atcatccccc cgcctccccc gccgcccccg ccctgcggcc ccatcccg
//
LOCUS       DBNGZA010000135         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1191167, whole genome shotgun sequence.
ACCESSION   DBNGZA010000135 DBNGZA010000000
VERSION     DBNGZA010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1191167"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="AC16FU_02985"
     CDS             <1..1270
                     /locus_tag="AC16FU_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR2993372.1"
                     /translation="WERQLDHTEIRAKEQFLRQHSRDRQVALKFRDPFVSYLEGILGR
                     GDRNLGKVVYRAWQKGARFDAWTEHFDYARWQTAFEECGVDPRFYTRELDPNWALPWD
                     HIDKGETRQRLLREREKSLVTTWETVAPFLESPTPGPENAKVSVAPPGEAGESPVSGN
                     GQGTASDLYGRGKKRVRAVVPLQVARSRVRLRWAKGPEVRFISHLDVMRTLERTIRRA
                     ELPVAYSEGFHPHMKLAFGPPRPLGFVSDDEYLDLQLTEPYTPALFARLSEQLPPGFT
                     VKNGRSILNKTFSLSQVINAARYAVDFTGDVPPERTDGWPEKITDILAARSWEITRRQ
                     TERTSEVDIRPHIYDLKWEKRAETTRINMLVGLGTGGYAKPQEILRFGLGLSEEEIAG
                     LVIRRTNLYVRRQARLDPGQFEWLSPLEVV"
     gene            1263..>1861
                     /locus_tag="AC16FU_02990"
     CDS             1263..>1861
                     /locus_tag="AC16FU_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR2993373.1"
                     /translation="MCKIAPTVRSSRSSAGLKSRQIALELLLGLERSREDFFESLAAH
                     RLLAPLVPVDRRLVQELVSGACKMQRRLDFILNQFLTQKKTKLDPLVRSILRLALYQL
                     EFLTRVPDFAAVNSAVELARERALGRKADFLNGLLRSYLRNREKLAYPERERNPNEYL
                     GIYYSYPDWLVARWVDEFGPEETEKLCAYGNRKSSLTLRV"
BASE COUNT          373 a          563 c          563 g          362 t
ORIGIN      
        1 gtgggaaagg cagctggacc acaccgaaat ccgggcgaaa gaacaattcc tgcggcagca
       61 cagccgggac cggcaggtgg cgctcaagtt ccgcgacccc ttcgtctcct acctcgaagg
      121 gattttgggc cggggcgacc ggaacctggg gaaagtggtc taccgcgcct ggcagaaagg
      181 cgcccgcttc gacgcctgga ccgaacactt cgactacgcg cgctggcaga ctgcgttcga
      241 ggagtgcggc gtcgatccac gattttacac gcgcgagtta gacccaaatt gggctttgcc
      301 ctgggaccac atcgacaaag gtgaaacgcg ccagcggctc ttgcgggagc gggaaaagtc
      361 gctggtcacc acctgggaga cggtggcgcc ttttctggaa tctcccacgc ccggccctga
      421 gaatgcaaaa gtctcggtgg cgccccccgg cgaagcggga gagtcacctg tatccggcaa
      481 tgggcaaggc actgcctctg acctctacgg ccgggggaag aaaagggtgc gcgcggttgt
      541 tcctttacaa gtcgcgcgca gccgggtgcg cctgcgctgg gccaaagggc cggaagttcg
      601 cttcatttct catttggacg taatgcgcac cctcgagcgg actatccgtc gtgctgaact
      661 tccggtggcc tattcggaag gtttccaccc gcacatgaaa ttggccttcg gtccgccgcg
      721 gcccctggga ttcgtttcgg atgatgagta cctcgacctg cagctcaccg agccctacac
      781 cccggctttg ttcgcgcggc tctccgaaca acttccgccc ggcttcaccg tgaaaaacgg
      841 aaggagcata ctgaacaaaa cgttttcgct ctcgcaggtg atcaacgccg cccggtacgc
      901 cgtggacttc accggtgacg ttcccccaga gcgcaccgac ggctggccgg agaagataac
      961 agacatactg gcagcgcgct cctgggagat tacccgccgc cagaccgaaa gaacctccga
     1021 ggtcgatatc cggccccaca tttatgacct caagtgggaa aaacgggctg aaacgacacg
     1081 aatcaacatg ctggtggggc tggggacggg cgggtacgcc aagccgcagg aaattctgcg
     1141 cttcgggttg gggttgagcg aagaggaaat tgccggattg gtgatccgcc gcacgaatct
     1201 ctacgtccgc cggcaggccc gtctggatcc ggggcagttc gagtggctct cgcccctgga
     1261 ggttgtgtaa gattgctccg acggtccgct cgtcccgttc ttccgctgga ttaaaatccc
     1321 gccagatagc ccttgaactg ctgctcggac ttgagcgcag ccgggaagat tttttcgaat
     1381 ctctggctgc gcaccggctc ttagcgcccc tggttccggt tgaccgccgc ttggtgcagg
     1441 agttggtttc cggcgcctgc aagatgcaga ggcggctcga ttttatcctg aaccagttct
     1501 tgacgcaaaa gaaaaccaaa ctcgatcccc tggtgcgcag catcctgcgt ctggctctct
     1561 accagctgga gttcttaacc cgagtgcccg atttcgccgc cgtcaattca gccgtagaac
     1621 tggccaggga gcgcgccctg ggccggaaag ctgacttcct caacggcctt ttgcgttcct
     1681 acttgcgcaa tcgggagaaa ctcgcttacc cggagagaga gagaaatccg aacgaatatc
     1741 tgggaattta ctactcctac cccgactggc tggtggcgcg ctgggtggac gaatttggcc
     1801 cggaggaaac cgaaaagtta tgcgcctacg gcaatcgtaa gtcctctttg accctgcggg
     1861 t
//
LOCUS       DBNGZA010000136         3415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1509271, whole genome shotgun sequence.
ACCESSION   DBNGZA010000136 DBNGZA010000000
VERSION     DBNGZA010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1509271"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16FU_02995"
     CDS             complement(<1..380)
                     /locus_tag="AC16FU_02995"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR2993374.1"
                     /translation="MNSILILTGPTAVGKTQLALEIADNRGGEIISADSRQIYRFLDI
                     GTAKPSAEIRSRVPDYLLDILDPTERYSAARFRADALRLIREIAGRGRWPLVVGGTGL
                     YLRALTQGLFQGPGADEKVRERLH"
     gene            complement(423..2270)
                     /gene="mutL"
                     /locus_tag="AC16FU_03000"
     CDS             complement(423..2270)
                     /gene="mutL"
                     /locus_tag="AC16FU_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR2993375.1"
                     /translation="MQIASSSQRIKILPEELANKIAAGEVVERPASVVKELVENSLDA
                     GATQITIDLKQSGFAEIRVTDDGSGMNREDALLALQRHATSKITSLDDLTRITTLGFR
                     GEALPSIAAVSLLELTTCAGEGTGGTRLVCEAGKVTKVSDIGAPQGTTVTVRELFFNT
                     PARRKFQKSPMAEIRQISEVVTQIALGNSGVAFALTNDGRELLKVPGKQTTVDRLGRL
                     FGKGWAAQLVPLSENLEGVRLDGFAGKPDFSKSNRAQLYLFVNRRPIQSRLLYHAVAA
                     GYGELLPRGRFPVAVVFLTIDPELVDVNVHPTKNEVRFASDRLLHSLLYSAVSRVMQA
                     GGGEKQFATPAVREQSVIPQPLPELVLTGTLSLPVQPDERKSALAAEKAAPLPLPDSR
                     QRLESPAPPSPATLESSEKVSAPLESSQVTADHKPESGTFYQFANVYIISQVKGSLVV
                     VDQHTAHERVLYEEALRMFAASGAHSQQLLFPISLELSPVEYATFTENQDVLAKLGFE
                     LQPFGDRTVLIAGMPMLVREKNPEKFLREVLDDLAENLKAGGDRLKAVAQSYACKAAV
                     KAGDKLTPEEMSALFDQLFATRNPYVCPHGRPTVVRIPVEELNKKFGRS"
     gene            complement(2391..2813)
                     /locus_tag="AC16FU_03005"
     CDS             complement(2391..2813)
                     /locus_tag="AC16FU_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR2993376.1"
                     /translation="MPEPKIRVLLAKPGLDGHDRGVKVIAAAMRDAGFEVIYAGLRQT
                     PEMVVEAAIQEDVDAIGLSILSGAHMTIFPAVLKLLKERGAGDVILFGGGIIPDEEMK
                     QLEALGVGKLFTPGAPTSEIIEYVRKAVKGRKKPAAIL"
     gene            complement(3025..>3415)
                     /locus_tag="AC16FU_03010"
     CDS             complement(3025..>3415)
                     /locus_tag="AC16FU_03010"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2993377.1"
                     /translation="KPIVCACLEDITFYCKRFEVKPHGWVIMPDHVHLLATFARREII
                     SQFVHGVKSHFAHRLRQGSTWQKGCYDHGIRNEKDFFEKLNYIHNNPVTAGLVSDPGE
                     YRFSSYRTYGYDEEGLLPIDKLLLLKA"
BASE COUNT          690 a          992 c          961 g          772 t
ORIGIN      
        1 ttgtgcaacc tttccctcac tttttcatcg gctcccgggc cctggaacaa tccctgggtg
       61 agcgccctaa ggtaaaggcc ggttccaccc actaccagtg gccaccggcc gcgtcctgcg
      121 atttccctta tcaaccggag cgcgtcggca cggaagcgcg ccgccgagta gcgctcggtc
      181 gggtcaagga tgtctaataa gtaatccgga acacggctcc ttatttcggc actcggtttg
      241 gccgtcccga tgtccaaaaa acgatagatc tgccgggaat cagccgagat gatttcacca
      301 cctctattat cggcaatttc aagcgccagt tgagttttgc ctaccgcggt ggggccggtg
      361 agaatgagga tgctgttcaa gttttgagca gaattactgg taagcaatga tggacaaaag
      421 gatcacgatc gaccgaattt tttattcaac tcctccaccg gaatccgcac aaccgtcggc
      481 ctcccgtggg ggcagacgta ggggttccgg gtggcgaaga gctggtcgaa gagcgcgctc
      541 atctcctccg gggtcaactt atccccggct ttgaccgccg ccttgcaggc gtaggattgc
      601 gccaccgctt tcagccggtc gcctcccgcc ttgagattct ccgccagatc gtctagcact
      661 tcgcgcagga acttttccgg atttttctcg cgcaccagca tcggcattcc ggcaatcaag
      721 accgtacggt caccgaacgg ctgcagttca aaacccagtt tcgcgaggac gtcttgattc
      781 tccgtaaacg ttgcgtactc aaccggcgaa agttccagtg aaatcggaaa gaggagttgc
      841 tgggagtggg cgccagaggc ggcgaacatc cggagcgcct cctcgtagag tacccgctcg
      901 tgggcggtgt gctgatcgac gacgaccaga ctgcccttca cctgcgagat gatgtaaacg
      961 ttggcaaact ggtagaaggt tccgctctca ggcttgtggt cagcggtgac ctgtgaagat
     1021 tccaacggcg cgcttacctt ttcactcgac tcaagcgtcg ccgggctggg aggcgccgga
     1081 ctctctaacc tttgcctgga gtcggggagc ggaagcggcg cagctttttc tgctgcaagt
     1141 gctgattttc gttcgtcagg ctgtacgggg agcgaaagtg ttccggtgag caccagttcc
     1201 ggcaggggtt gagggataac gctctgttcc cgcaccgcgg gagtcgcgaa ctgtttctct
     1261 cccccgcccg cttgcatcac ccggctcacc gccgaataga gaagactgtg gagcagccgg
     1321 tcgctggcga agcggacttc gtttttggtc gggtggacgt tcacgtctac cagttccgga
     1381 tcgatggtga gaaaaaccac cgctaccgga aagcggcccc gaggcaaaag ttcgccgtac
     1441 ccggctgcaa ccgcgtggta tagcaagcga ctctgaattg ggcggcggtt gacgaagagg
     1501 tacaattggg cgcggttcga tttggagaaa tcgggtttcc cggcaaaacc gtccaggcgt
     1561 actccttcga gattttccga caaaggcacc aactgagccg cccacccttt gccgaacaat
     1621 ctccccaggc gatcaacagt ggtttgcttg ccgggaacct tgagcaattc acggccgtca
     1681 ttggtgaggg caaaggccac tcccgaattt cccagcgcta tctgcgtcac cacttcagag
     1741 atttgccgga tttcggccat cgggctcttc tggaatttcc tgcgggccgg ggtattgaaa
     1801 aagagttcgc gcaccgtgac ggtcgtgccc tgtggcgctc caatatcaga tacttttgtg
     1861 actttgcccg cttcgcacac cagccgagtt ccgcctgttc cttcaccggc gcaggtggtc
     1921 aattctaaca gggacacagc ggcgatcgac ggcaaggcct cgccccgaaa gccgagcgtg
     1981 gtgatgcggg tcaggtcatc cagcgaagtg atcttgctcg tggcatggcg ctggagggcc
     2041 agaagggcat cttccctatt catcccggag ccgtcatccg tcacgcggat ttcggcgaag
     2101 ccggattgct tgaggtcaat ggtaatttgg gtggctccgg cgtcgaggga attttccacc
     2161 aattccttga ccaccgaagc cgggcgctcc accacctcgc ccgcggcgat tttattggcc
     2221 agctcttcgg ggaggatttt aattcgctgc gacgaagatg caatctgcat gtctgtgatg
     2281 gaatgagctt gtctcattaa caccaaattt cgggcgaccg ggggcggtcg ccctacgcga
     2341 ggcgtttttc tttttcccgc gtaggcgcgg cgccctcgcc gcgctcatcg ctaaagaatc
     2401 gccgctggct tcttccgccc tttaaccgct ttccggacgt actcaataat ttccgaggtc
     2461 ggcgcgccgg gggtgaacaa cttccctacg cccagggctt cgagttgttt catctcttcg
     2521 tccggtatga tgccgccgcc aaagaggatg acgtcaccgg ccccacgttc tttgagcagt
     2581 ttcagtaccg ccgggaagat cgtcatatgc gcgccggaaa gaatcgagag cccgatggcg
     2641 tccacatctt cctgaatggc ggcctcgacc accatttccg gagtctgccg cagtccggcg
     2701 tagattacct caaagccggc gtcgcgcatc gctgcagcaa tcaccttgac gccgcggtcg
     2761 tggccgtcga ggccgggttt ggctaaaagg acgcgaattt tcggttcagg catatcatct
     2821 tttccctctc tacctacttg cggaattttc gccttcgcct gagcgatcaa accgaaaaat
     2881 aggtggaagt aaaatatgtg aaaggactct gtttgcaaac ccctgctggt acgggcgacc
     2941 ggggggggag gtcgccctac gcggcggact gtctcgcttc gccacgatgg cttttttccg
     3001 cgtaggcgcg gtgccctcgc cgcgctatgc cttcagaagc aacaacttat cgataggtaa
     3061 caagccctct tcatcatacc catatgtccg atagcttgaa aatcgatact cgccggggtc
     3121 actgaccaga ccggcggtca ccggattgtt gtggatgtag ttcagtttct caaagaaatc
     3181 cttctcgttc cgaatgccgt gatcataaca ccccttctgc caggtcgaac cttgtcgcaa
     3241 acggtgagca aagtggcttt taaccccgtg gacaaattgc gaaataatct ctcttcttgc
     3301 aaaggttgcc agaagatgca cgtggtcggg cataatcacc caaccgtgtg gttttacttc
     3361 gaaacgctta caataaaagg tgatgtcctc taaacaggcg caaacaattg gtttc
//
LOCUS       DBNGZA010000137         1665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1404389, whole genome shotgun sequence.
ACCESSION   DBNGZA010000137 DBNGZA010000000
VERSION     DBNGZA010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1404389"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1007)
                     /locus_tag="AC16FU_03015"
     CDS             complement(261..1007)
                     /locus_tag="AC16FU_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2993378.1"
                     /translation="MPKVFLLIDSRVWQLVVDADPVAKGVLAILAFISVLSWAVIINK
                     IRVFRRARSESEQFLASFRRRRRIADVYANLVSLRHTPLARIFAEGFKDLEHLQRKEA
                     RNPGPVSIVRGEAVRQAAPPEDELLGGSQIDALRLTLSRVAGEELARYEKNVTVLATT
                     GNVAPFFGLLGTIWGVMDAFLSIGIRGQANLAVVAPGIAEALIATIVGLAAAIPAVMG
                     YNYCVSRLRELSGQMDNFTLELLTAFLKEQ"
     gene            1338..1514
                     /locus_tag="AC16FU_03020"
     CDS             1338..1514
                     /locus_tag="AC16FU_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993379.1"
                     /translation="MKKFLAVLLIGLVGLVVIAGCGKKEEKTAPATETAAPAPTDTTH
                     AMDTTKAADTTKTK"
BASE COUNT          368 a          501 c          481 g          315 t
ORIGIN      
        1 ctcccaatgt aggggctggg tctcccagcc cagagcggtt ctatcattcc gtaggggcga
       61 cccgccgggt cgcccggcct tgttctttta atccttctgt atgggcgaac ccccgtgttc
      121 gcccgcgtct ctgattattt gggggtgaat ccgcctcagg cggattcatc ctgtttcttg
      181 gggcgaatct aaagattcgc ccctaccaca ccgtgggcgc ggcgccctcg ccgcgcactc
      241 caaataatgc ctgatggcac tcactgctct ttgagaaacg ccgtcagcaa ctccagcgta
      301 aaattatcca tctggccgga gagctcccgc agccgggaga cacagtagtt ataccccatc
      361 acggccggaa tcgccgccgc cagcccgacg atggtggcga tgagcgcctc cgcaatgccg
      421 ggagcgacga cggccaggtt cgcctgcccc cggatcccga tcgaaagaaa cgcatccatc
      481 acgccccaga tggtccccag aaggccgaag aatggcgcca cgttgcccgt ggtggcgaga
      541 acggtgacgt tcttctcgta gcgcgccaac tcttcgcccg ccacccgcga cagggtcagc
      601 cgcagggcgt caatttgact cccccccaga agttcgtcct caggaggagc cgcttggcgg
      661 accgcctcgc cgcgcacgat agagaccggg ccgggattgc gcgcttcctt ccgctgcaga
      721 tgttccaaat ccttgaaccc ttcggcaaag atgcgcgcca gaggcgtatg ccggaggctg
      781 actaaattgg cgtagacgtc ggcaatccgc cggcggcggc gaaacgaggc cagaaactgc
      841 tccgactcgc ttctggcccg gcggaaaact ctgattttat tgatgatcac cgcccaggag
      901 agcaccgaaa tgaaagccag aatggccagg acccccttgg ccaccgggtc ggcatcgacg
      961 actaattgcc agacgcggct gtcgatcaaa agaaaaactt tcggcatacc tcctccttca
     1021 tacgagtcgt tcggacggaa aggcggtatt ttcttgtcgg tcaatcggtt ttgaccgccg
     1081 gcacgacccg caggagacaa aataggccgg ctctccggag cggtcaaggc caaagagaaa
     1141 aaaggtctgc aagaaaaatt atttcggaat gccgggaaag ggcttgacgg gctggcacgc
     1201 ccctctatat tagcgcctta attttgtttg cggataacag ggatgcaaat cattccaaaa
     1261 gtgccaaaca cgcactgcgg gtcgaatatg actcccaagg gtcgtatcga ccgtgccggg
     1321 ggaggtgaga gtaaaggatg aagaagtttt tggccgttct tttgatcggc ttggtcggtc
     1381 tggtcgtcat cgccggctgc gggaagaaag aagagaaaac cgcgcccgcc accgagaccg
     1441 ccgcgccggc tccaacggac accacacacg cgatggacac cactaaggcc gccgacacta
     1501 cgaagacgaa gtaactccac gacgaatctc cgaagacgaa ggcagtgagg agcagaggca
     1561 ctgccttttt gtttttggtg gtgcgccacg tccggctggg aaagatttct taaaacacgt
     1621 agcgcgggtt ggtttgcgta acccgcgcca tgtgggaaca cattt
//
LOCUS       DBNGZA010000138         5724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2888192, whole genome shotgun sequence.
ACCESSION   DBNGZA010000138 DBNGZA010000000
VERSION     DBNGZA010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2888192"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1522)
                     /gene="dnaA"
                     /locus_tag="AC16FU_03025"
     CDS             complement(65..1522)
                     /gene="dnaA"
                     /locus_tag="AC16FU_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR2993380.1"
                     /translation="MLKSEQEIWTDCLAYLEKRIKKQSFYTWLRPTRAERLMNDKLVV
                     SVPNRFVVEWLEEHYLPLIHEALLAVTGKSLAVILQIGANGGGAQLSESNGQVRSFAD
                     QRTAAQEPVHGESRSANSSASFHPKTIDPRVVKGAAPGDGQLNLRYTFDTFVVGESNQ
                     FAHAAALAVAESASGGAGQTKYNPLFVYGGTGLGKTHLAQAIGHYVKELTPQARILYV
                     TSEKFTSDFITAIGHNTANAFAAFYRQAELLIIDDIQFFTGKESTQMQFFHTFNALYH
                     NGKQIVLTADRAPKEIRGLEDRLLSRFSWGLVTDIQPPDMETRLAILKKRTAVEQIGL
                     PEDVLEFVALRVTSNIRELEGALVRLLAYASLARKDINLEVAQTILKDTLSNGSARSV
                     TIEQIKQGVANEFGVPLEVLVAKRKTQEVALARQVAMYLARTLTSSSLKTVGEKFGGR
                     DHSTVLHACNVVAQQFKSNADFKRRIDGLITSLYV"
     gene            complement(1875..2222)
                     /locus_tag="AC16FU_03030"
     CDS             complement(1875..2222)
                     /locus_tag="AC16FU_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993381.1"
                     /translation="MTGKFAAQSFSILDRFVNKKKKGVHLFDIRVNYFADRDDYHFLV
                     PEKNTLHSSSNDLAFGKAAWSFHIAQKKFCSHRDLVDLKLFYPIDRLSPVIDYALCEQ
                     RKTCGVSNAGHVN"
     gene            complement(2229..3611)
                     /gene="thiC"
                     /locus_tag="AC16FU_03035"
     CDS             complement(2229..3611)
                     /gene="thiC"
                     /locus_tag="AC16FU_03035"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR2993382.1"
                     /translation="MSVNGSCPEYCFSQMHYAKRGIITDAMRRVAEGEDQNPELIRAE
                     IARGRLIIPANVNHRSLVPMGIGIAVKCKINANIGNSAVISKIDDELAKLHLAVKFGA
                     DTVMDLSTGGNIDKIREAIIAASPVPVGTVPIYQAVAGVKKVEALTADDLVGMIEHQA
                     KQGVDYMTVHAGVLREHLPLVQGRITGIVSRGGALLAQWIISHRQENPLYAHFDKILE
                     IARRYDVTLSLGDGLRPGCLADASDKAQFAELKTLGELTLRAWEKDVQVMIEGPGHIP
                     IDQLQMNVEMEQQLCHEAPFYTLGPLVTDIAPGYDHITSAIGAAMVGFYGASLLCYVT
                     PKEHLGLPEFEDVKQGVIAYKIAAHAADIARHRKGARERDDALSRARFAFDWERQFNL
                     SLDPETARKYHDLTMPHPVHKEAEFCSMCGPKFCSMHISKAVENWAVALLPAEESTPL
                     KELQESRPGT"
     gene            complement(3715..4341)
                     /locus_tag="AC16FU_03040"
     CDS             complement(3715..4341)
                     /locus_tag="AC16FU_03040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2993383.1"
                     /translation="MPVDTLKVPIVLSVGRLVEGKGHMYLIRAMKMLRRETELIIVGT
                     GPERDRLIAEARTAANGSPPTFRFTLVENPTQEKLHTLYDQASVFVLPSVSDRRGWKE
                     ALGMVLLEAIAHRLPVVAFDSGGISDVIQDGVTGFLVKEGDEPGLANQIADALDGKLN
                     VETALRVTTAKFSRRKIQETVLKALAETLARNRAGGAAHQPARMPADR"
     gene            complement(4323..4820)
                     /locus_tag="AC16FU_03045"
     CDS             complement(4323..4820)
                     /locus_tag="AC16FU_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR2993384.1"
                     /translation="MQTLLVLTHCYPQNERDSAGIFVKDWVEMVRDGGYKVEVLVCEG
                     LAGKLTRWRGLLKAIGLLPSCFWSAFRKARAVDLVAAHWIFPSGLLAVVVGFLRRKPV
                     YVIMHAEPFVIEKRRWVRVIAKMILHIPAHVTVQVVSTPCRKIVEQIYRGRLVVAPMP
                     CRLIP"
     gene            complement(5044..>5724)
                     /locus_tag="AC16FU_03050"
     CDS             complement(5044..>5724)
                     /locus_tag="AC16FU_03050"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HIR2993385.1"
                     /translation="VESNNYLGGGFWIGGYLMNGVTVRRPAQAILDEIGAPYQEVAPG
                     LYLSPGPHACSKLVAAACDAGVTVINMSKFDDVVLREGNRVAGVVVNWTPVSALPRAI
                     TCTDPVALEAKVVIDATGHDAVVCEALKRRKLLDYGTYGAMWVERSEDLVVEKTCEVH
                     PGLIVAGVAVATVFEIPRMGPTFGAMLLSGKKAAQIAKQLLPRKAAVSGNGQPKTGRT
                     KPALAGVK"
BASE COUNT         1205 a         1666 c         1547 g         1306 t
ORIGIN      
        1 ggaaatgaaa gatatccaca acgggactgt tatgcgtcgg gtttgaactt attatccccg
       61 catgctacac atagagtgag gtgataaggc cgtcaattct ccgtttgaag tcggcgttgc
      121 tcttgaactg ttgggcgacg acgttgcagg cgtggagcac cgttgagtgg tcacgcccgc
      181 cgaacttctc accgaccgtt ttgagcgaac tactggtcag tgtccgggcc aagtacattg
      241 cgacctgtcg ggccagggcg acttcctgcg tttttctttt ggcaaccagc acctctaaag
      301 gaaccccaaa ttcattggcc accccctgtt ttatctgttc aatcgttacc gaccgggccg
      361 atccgttgga gagagtgtct ttgaggatgg tttgcgctac ctcaaggtta atgtctttac
      421 gtgccaagga agcatacgct aaaagccgga ctaaggcccc ttccaactcg cggatgttgg
      481 aggttacccg cagagctaca aattccagca catcttccgg aaggccgatc tgttccacgg
      541 cagttctttt tttgaggatc gcgagtctgg tctccatatc cggtggctga atgtccgtca
      601 ctaaacccca ggagaagcgg gaaagcaggc gatcctcaag cccccgaatc tccttcggag
      661 cccggtcggc ggtcaagaca atttgtttcc cgttgtggta cagggcgttg aaggtatgga
      721 agaactgcat ctgggtcgat tctttgccgg tgaaaaattg gatatcatcg atgattaaaa
      781 gttcggcttg gcggtaaaac gcggcgaagg cattcgcggt gttatggccg atggcggtaa
      841 tgaagtcgct ggtaaatttt tcagaggtga cgtaaagtat ccgggcctgg ggcgtcagct
      901 ccttgacata gtgcccaatg gcttgcgcca aatgggtctt ccccagaccc gtgccgccat
      961 aaacgaaaag cggattgtat ttggtctgtc ccgctccgcc ggaagcggat tccgcgacgg
     1021 ccagagcggc agcatgcgca aactggttcg attcaccaac gacgaacgta tcgaaagtgt
     1081 accgcaagtt aagttgtccg tcacctgggg cggcgccttt cacaacccgg ggatcgatag
     1141 ttttcggatg aaaactcgcc gacgagtttg cgcttcgcga ttcgccgtga accggttctt
     1201 gagcggcagt ccgttggtct gcaaaagacc gcacttgacc attcgattca gataattgtg
     1261 cacccccgcc attcgcacct atctgcaaaa tcacagcgag cgacttgccg gttaccgcca
     1321 acagcgcctc gtggatcaac gggaggtagt gttcttccaa ccactcaacc acaaagcgat
     1381 tgggaacaga cactaccaat ttgtcgttca tcagacgctc ggccctggtt ggccgcagcc
     1441 aggtgtagaa gctctgcttt ttgatccgct tttctaaata agcgaggcaa tccgtccaaa
     1501 tttcctgttc acttttcaac attttttcca cagacaatgg gggaaaactt tgagcaatca
     1561 tttatttgca caccgactcg catttggagc actcaagaaa ctgaaagtca tttcggtcaa
     1621 gccgttgtgg aaaaagctaa ctcgaagagc ggtaagccgt taataaaaaa atcaaaacac
     1681 ttttccaccc attctccaca gcaaagggcc ttgcccacaa aggaaaatta cctgtcagaa
     1741 caataagttc ccgcccaccc aatcagatcc aacgattttt ccttgttcgg taataagcac
     1801 gagttgttga gttggtaaat aactcgtgat gagcgatgct gtcaagagtt tttcttcttg
     1861 ttcgcacaaa aaagttaatt gacgtgcccc gcattactta cgccgcatgt ttttctttgt
     1921 tcgcatagtg cgtagtcgat caccggggaa agtcgatcga ttggataaaa caatttcaag
     1981 tcgactaagt ctctatgaga gcagaatttt ttctgggcaa tgtgaaaact ccaagccgcc
     2041 ttcccgaacg ccagatcatt tgacgaggag tgcaaagtgt ttttctctgg aacaagaaaa
     2101 tgataatcat cccgatccgc aaaataattt acccggatat cgaaaagatg tacccccttc
     2161 ttctttttgt tcacaaagcg atcaaggatc gagaaacttt gggccgcgaa cttgccggtc
     2221 aaacgttttt aggtcccggg ccgcgattcc tgcagctctt tcaggggcgt tgattcctcc
     2281 gccggcagca gcgccaccgc ccagttttca accgccttgg aaatgtgcat cgagcaaaac
     2341 ttcggcccgc acatggagca gaattcggcc tctttgtgaa cggggtgggg catcgtcagg
     2401 tcgtgatact tccgggcggt ctccggatcc aaagaaagat taaactgccg ttcccagtcg
     2461 aaagcaaacc gggcccgcga cagagcgtcg tctctttccc gcgctccctt ccggtgccgg
     2521 gctatgtcgg cggcgtgggc cgcaattttg taggcaatta ccccttgctt cacatcttcg
     2581 aattccggca accctaagtg ttccttgggg gtaacgtagc agaggaggct ggcaccgtaa
     2641 aatcccacca tagccgcgcc gatggcggaa gtaatgtggt cataccccgg tgcgatgtcg
     2701 gtgaccagag gccccagcgt gtagaaaggg gcctcatggc acagttgctg ttccatttcc
     2761 acgttcatct gcaactggtc aatcggaatg tgccccggtc cctcgatcat cacctgcaca
     2821 tctttctccc aggcccgcag ggtcaattcc ccgagcgttt tcagttcggc gaattgggct
     2881 ttgtcggaag catcggcgag gcagccgggc cggaggccgt cgcctaaact caaggtgacg
     2941 tcgtaccgcc gggcaatctc cagaatcttg tcgaagtgcg catacaacgg attctcttgg
     3001 cgatgtgaga taatccattg tgccagaagt gctccgcccc ggctgacgat gccggtaatc
     3061 cgcccctgca ccaaaggcag gtgttcccgc aggactccgg cgtggaccgt catgtaatcg
     3121 actccctgtt tggcctggtg ctcgatcatc ccgaccaagt cgtcggcggt cagggcttcg
     3181 acttttttga cgcccgccac ggcctgataa atcggcaccg ttcccaccgg aaccggactg
     3241 gctgcaataa tcgcctcccg aattttgtca atgttcccac cggtggagag gtccattacc
     3301 gtgtcggcgc cgaatttgac cgccaggtgc aacttggcca gctcgtcgtc aatttttgag
     3361 atgaccgccg agttcccgat gttggcgttg atcttgcact tgacggcaat gccgattccc
     3421 atcggcacca gactccggtg gttgacgttg gccggaatga tcagccggcc gcgggcgatt
     3481 tcggcgcgga ttagttccgg attttggtcc tctccttccg ccactctccg catggcgtcg
     3541 gttataatcc cccgcttggc atagtgcatt tggctaaagc aatactccgg gcacgatcca
     3601 tttactgaca taacggagac ctctcgcgac aggccccgcc gatagcaggg cacgctttct
     3661 ttataccgcg gcgtggtttt tttatcgggt tcatggttcc acgccctacg tcgattatct
     3721 gtctgcaggc atccgggctg gctggtgcgc tgcaccgcct gcccgatttc gcgccagagt
     3781 ttcggccagt gcttttagaa cggtctcttg aatttttcgg cgcgagaatt tggccgtcgt
     3841 tacccgcagc gccgtttcca cgttcaattt gccgtccagc gcatcggcaa tctggttggc
     3901 cagtccgggt tcatcgccct ccttgaccag gaagcccgtt acgccgtcct ggatgacgtc
     3961 ggagatgccg cccgagtcaa acgccaccac gggcaggcgg tgggctatgg cctccaggag
     4021 caccatgccc agagcctcct tccacccgcg ccggtcactg acgctcggca ggacgaaaac
     4081 cgaggcttga tcgtagaggg tatgtagttt ttcctgcgtc gggttttcca ccagggtaaa
     4141 ccgaaacgtc ggcggcgaac cgttcgccgc agttctcgct tcggcgatca ggcggtcccg
     4201 ctccggcccg gtaccgacga taataagttc agtctctcgc cgcagcatct tcatggcgcg
     4261 aatcaggtac atatggccct tgccttcgac caaccggccc acggagagga caattggcac
     4321 cttcagggta tcaaccggca tggcatcgga gcgacgacga ggcggccccg gtagatttgt
     4381 tcgacgattt tgcggcaggg ggtggagact acctgcaccg tgacgtgcgc cgggatatgg
     4441 agaatcattt tcgcgatgac gcgtacccag cgccgctttt cgatgacgaa cggttcggcg
     4501 tgcatgatga catacaccgg tttgcgccgg aggaagccga cgaccaccgc cagaagaccg
     4561 gacggaaaaa tccagtgggc ggccactaaa tcgacagccc gcgcttttct gaaagcagac
     4621 cagaagcagg aggggagcag gccgatggcc ttgagcaggc cccgccaacg ggtcaacttg
     4681 cccgccaatc cctcacacac aagcacttcg actttgtacc ccccgtctct taccatttcc
     4741 acccaatcct tgacgaatat tccggcactg tcgcgctcat tttgggggta gcagtgcgtg
     4801 aggaccaaga gggtttgcat acgctacaat cggcaagcga acaaagggta aatccacacg
     4861 ttgtcctgca gtataaagtg aactcgtttt tctgtccacg cggcgttgtt ctccgttttc
     4921 aagcggctaa ggatgtacgc cggaacgaga ttgatgaact cccgcagtaa ccaatgtggc
     4981 ggggcggcgg cgttgccacc cctggttgct gggaagttct ccgccttctt ttcagtgctt
     5041 cccttacttg actccggcca gagccggctt agtcctgcca gtctttggct ggccgttgcc
     5101 ggagaccgcc gccttccggg ggagcaactg tttggctatc tgcgccgctt ttttgccgga
     5161 gaggagcatc gccccgaaag tcggccccat ccgcgggatt tcgaacacgg tggcaaccgc
     5221 cacgccggcg acgatcaacc ccgggtgcac ttcgcaggtt ttctcgacca ctaaatcttc
     5281 cgaccgctcc acccacatcg cgccgtaggt accgtagtcc aacaacttgc gccgcttgag
     5341 cgcctcgcag accaccgcat cgtggccggt ggcgtcgatg acgaccttgg cttccagggc
     5401 caccggatcg gtacaggtga tggcgcgggg cagcgccgaa accggcgtcc agttcaccac
     5461 cacgccggcc acccgattcc cttcccgcag caccacatcg tcgaattttg acatattgat
     5521 caccgtcacc ccggcgtcgc aggcggcagc gacgagtttc gaacaggcgt gcggtccggg
     5581 cgaaaggtag agaccgggtg cgacctcctg gtacggcgcc ccgatttcgt ctaagatcgc
     5641 ctgtgccggc cggcggacgg tcaccccgtt catcaggtag ccgccgatcc agaacccgcc
     5701 gcccaagtag ttgttgctct ccac
//
LOCUS       DBNGZA010000139         5102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2569973, whole genome shotgun sequence.
ACCESSION   DBNGZA010000139 DBNGZA010000000
VERSION     DBNGZA010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2569973"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1950
                     /locus_tag="AC16FU_03055"
     CDS             235..1950
                     /locus_tag="AC16FU_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2993386.1"
                     /translation="MTGVFSISAFGVDAPAVSKISPELLTRFQSGNPGATAKVWVFFT
                     DKGIADPAVLAKATADLSEYFSFRAIARRQKVRPGQLLFDETDLPVSADYLSLLSNSG
                     ITIHEVSRWLNAASISMTYAQALTVANLAFVREILPVMRTVQRLPAIEPVPRIEFAPA
                     APGALNYGPSFRQVSQIRANILHRLGYTGKGVLIGMLDTGFFIDHPVFDSLRLQGRIV
                     ATRDFINGDADVQDNAFDGQRDHGTATFSLLGGFADGTLIGTAFGASFALAKTEIVAT
                     EYIISEEDRWVKGLEWLDSLGADIVSSSLGYNVGDTGFSYTPAQMDGNTATTTKAADL
                     AASKGLLVVNAAGNEGNDPWRIIIAPADGDSVLAVGAVDSLGFHTSFSSTGPTADGRI
                     KPDVMAQGEDDWLASASGGYGRGGGTSFATPLAAGVAALLLEIDSTLTPFQIIQRLHQ
                     SASQASRPDNLYGWGIVNAARAAGFPEQPPVHRAQIRPNPVRTTATIDVTLDQPGKVE
                     LSIHTLAGERLWELVTNAAGTGAQTYFWDGRNQLGQPVAGGLYLLLIKTTSFQDVVKF
                     ALTRP"
     gene            2063..3670
                     /locus_tag="AC16FU_03060"
     CDS             2063..3670
                     /locus_tag="AC16FU_03060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR2993387.1"
                     /translation="MESTTLTPKAGLARGCAIFKVDLKGRLVFLDDEAESLFGYSEAE
                     LYGRPLVNYLVPDDRQRLTGWLQRKRFEHPDWENCAVSIVNRQGEQIILDTIVSVHFS
                     GGVPANYQFVVRGVTIPKTEEETKSAVFLDFTSDLKGLYTAAGEGQALAQKFVATVVE
                     RFSWAWAGFFVAAEKEPLLVVQAGSPPAGFNQIGGKLKTLKGQKRVGAVAVNGSDPAE
                     FVAVAVEIGLKERGYLVAGPAQAANLRPVPREFLEHFSAVFGTLYASALQHERLEETG
                     KTLAGAEQERQHLQRLLEQKTKQMAEAADSLAHDLRAPLVAVQGFAKRLANECGSAIG
                     PQGQFSLLCLQENVEIMGKMIDDLLQFSRVTHTPEKPQPLPLREVVTDLLEEFRSTSS
                     QPVEWRGVEELPTVVFDKGKLTQVLRNLLGNAITYTEGKGHPKVTLGWTDRPEAWEIR
                     ISDNGIGIPRQYHKKIFEMFTRVPNAVTKKQPGTGMGLAIVKKVLEDHGGKIWVDSHE
                     GRGSTFHLVIPKPAAEGKLPPADERQP"
     gene            complement(3852..>5102)
                     /locus_tag="AC16FU_03065"
     CDS             complement(3852..>5102)
                     /locus_tag="AC16FU_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR2993388.1"
                     /translation="RTYRLLLLGDSSPVGLGLQSHSLAFGELLESLLEREYADRKRFE
                     LINAAVSGYTSEQIARFLELRGWDFKPDLVILYCGNNDASISGSYSDRQLLGQQKLRK
                     VRRLFSHLALYRVLRALLVQDRWSGTRPAETLQVRVPASRFGTNLTRIADQCRQHGCP
                     LIVLKPPVPYLWPAGLQFKLFTQVSGEDGRLIIPDAMQRILGRPIKYCLSPERFTALY
                     GRGDIFTRSVYRSAYSDSLPPQEAIAYYANLIATDSGNPVLFNNLGVLLWEDRRYWEA
                     DYYLKTARNLYLRHYRDSLNPTVESAGAPFLYNIGINLLSLENYGPNLLHDTARAAFQ
                     YLDSALQADYFSLRVKRTYWEKIDRLKGEKNLIVIDLPATFQAEGGEKLFIDHCHPTA
                     EGHRLIAEEILKVIRNLLSRQPTT"
BASE COUNT         1052 a         1442 c         1509 g         1099 t
ORIGIN      
        1 cgggatttac ttctaccgct tgcaggcggg tgagtttcgg gagaccaaac ggatggtgct
       61 cctgaagtag tttatccgac ctttctttct gcgtcaagct gaccctctga gttttctctt
      121 gcacaacggc gatttatttc gtaaaaattg ctgatgccct tctctgacca actctcccga
      181 gaaaccgcac aaccatactt tcggcggtta cgcattattt tatgtgctct cggattgacc
      241 ggcgttttct ctatttcagc tttcggcgtg gatgcacccg ccgtttcgaa aatctcaccc
      301 gaacttctaa cgcggttcca gagcggaaat ccgggagcga cggccaaagt ctgggtcttt
      361 ttcaccgaca agggaatcgc tgacccggcg gttctggcaa aggccaccgc tgacctgagc
      421 gaatattttt cgttccgcgc catagcgcgg aggcagaagg ttcggcctgg ccagctgctc
      481 ttcgatgaaa ccgatctgcc ggtttccgcc gactacctga gcctcctctc gaattccggc
      541 atcaccattc acgaggtctc ccgttggctc aatgcggcca gcatctctat gacgtatgct
      601 caagccctga cggtggcaaa tcttgcgttt gttcgggaaa tcctcccggt gatgcggacg
      661 gtgcagcggt taccagcgat tgagccggtt ccgagaatcg aatttgcccc cgccgcgccg
      721 ggagcgctga actacgggcc ctctttccgg caggtcagtc aaatccgggc gaatatcctc
      781 caccggttgg gctacacggg caagggggtg ctcatcggca tgctcgatac cggatttttt
      841 atcgaccacc cggtgttcga cagcctccgg ttgcaaggtc gtatcgtcgc cacccgcgat
      901 ttcatcaacg gcgatgccga tgtccaagac aatgctttcg atggccagcg ggatcacggg
      961 acagccacct tttcgctttt gggcggtttc gcggacggga cgctcatcgg cacggctttc
     1021 ggcgcttcat tcgctttggc caagaccgag attgtcgcca ccgaatacat catctccgag
     1081 gaagaccgct gggtgaaagg gttggagtgg ctggacagtc tgggagccga catcgtttcc
     1141 agttccttgg gctacaacgt gggagacacc ggcttcagtt ataccccggc gcaaatggac
     1201 gggaacaccg ccacgaccac aaaagcggct gacctggcgg cctccaaggg gcttctggtc
     1261 gtcaatgcgg cggggaatga agggaatgat ccttggcgca tcatcatcgc tcccgcggat
     1321 ggtgacagcg ttttggcggt cggcgcggtc gattcgctcg gcttccacac ctcattcagt
     1381 tccaccggcc ccacggcgga tgggcgcatt aagcccgacg taatggccca gggggaagac
     1441 gactggctag cctcagcgag cggtgggtat ggacggggtg gggggacatc ttttgccacg
     1501 cctttggcgg cgggcgtggc ggccctgctt ttggaaattg actcaaccct cacgccgttt
     1561 cagatcatcc aacgcctcca ccaatcagcc agtcaggcct cccgacctga taacctgtac
     1621 ggctggggta ttgtcaacgc tgcccgcgcc gccggatttc ccgagcagcc gccggttcac
     1681 cgggcgcaga ttcgtccaaa tccagtgcgt acgacggcaa ccattgacgt aactctcgac
     1741 cagccgggaa aagtcgaact gtccatccac accctggccg gggagcggct ctgggagttg
     1801 gtgaccaatg ctgccggcac cggggcgcaa acctacttct gggacggcag aaaccagctc
     1861 gggcaaccgg tggccggcgg tctctacctg cttttgatca aaaccacatc gtttcaagac
     1921 gtggtgaagt tcgcgttgac ccgcccgtag ttttcccctc tcttttcctt gagtttcgaa
     1981 gccattctgc cgatgaaatg gtgagagtgg ttcccgcggc gagcgcgggc ccgtcagaaa
     2041 acttcagacc ctattcggca ctgtggagag cactaccctt accccaaaag caggactggc
     2101 gcggggctgc gccatcttca aagtcgatct caaaggccgg ctggtctttc tggacgacga
     2161 agccgagtcg cttttcggct attccgaagc ggaactttac ggtcgcccgc tggtgaacta
     2221 cttagtcccg gatgaccgtc agcgcctgac cggctggctc cagaggaagc ggttcgaaca
     2281 ccccgactgg gaaaactgcg cggtttccat cgttaaccgc cagggggagc agatcatctt
     2341 ggacaccatt gtcagcgtcc atttctccgg aggcgtgccg gccaactatc agttcgtggt
     2401 gcggggggtc acgatcccca aaaccgaaga ggagaccaaa agcgccgtct ttttagattt
     2461 caccagcgac ctgaaaggac tttataccgc cgccggggag ggtcaggctt tggctcaaaa
     2521 gtttgtcgcc accgtcgtgg agcgcttcag ctgggcgtgg gccggatttt ttgtagccgc
     2581 cgaaaaggag cccctactcg tggtgcaggc cggcagtccg ccggcggggt tcaatcaaat
     2641 cggggggaaa ctgaaaacgc tgaagggcca aaaacgggtg ggcgcagtgg ccgtcaacgg
     2701 ttcggatcca gcagagtttg tggcggtcgc cgtcgaaatc ggactgaaag aacgggggta
     2761 tctggtggcc gggccggccc aggcggccaa tctccgtccg gtgccgcgag agtttttgga
     2821 gcatttctcc gcagttttcg gtacgctgta cgcttccgcc ctacagcatg agcggctgga
     2881 agaaacggga aaaaccctgg ccggcgccga acaggaacgt cagcacctgc agcgtctgct
     2941 cgaacagaaa acgaaacaaa tggccgaggc ggccgattcg ctggcgcacg acctgcgggc
     3001 gcctctggtc gcggtacagg gattcgccaa acgtctggcc aacgagtgcg gttcggccat
     3061 cgggccgcag gggcagttct ccctgctttg cctgcaggaa aatgtggaga ttatggggaa
     3121 gatgattgac gatctgttgc agttctcccg ggtgacccat actccggaaa agccgcagcc
     3181 gttgcctttg cgcgaggtcg taaccgacct cctggaggag ttccgttcaa cctccagcca
     3241 gccggtggag tggcgcggcg tggaggagtt gccgacggtc gtctttgaca aaggcaagtt
     3301 gacgcaggtc ctccggaacc ttttgggcaa tgccattacc tacacggagg gcaaaggcca
     3361 tccgaaggtg accttggggt ggaccgaccg gccggaagct tgggaaatcc gcatctccga
     3421 caacggcatc ggcattcccc ggcagtacca caagaaaatt tttgagatgt tcactcgggt
     3481 tccgaatgcg gtaaccaaaa agcagccggg gacggggatg ggattggcga ttgtgaagaa
     3541 agtcttggaa gatcacgggg gaaaaatctg ggtcgactcc cacgaggggc gggggagcac
     3601 cttccacctc gtcattccca aaccggcggc cgagggaaag cttccacctg cggacgagcg
     3661 ccagccctga cgttctccgt tcctgttccg gtcagtcctt taagatctgg ctatagagtg
     3721 ttcgacgcgg aacggctttt cgtcatcatc cgcaacatct tgaatttttc gggaaggatc
     3781 tcagtgactt cgccgaaaat tcggctccga tcttcagatg tatcccaact cggcctgtgt
     3841 ggtttaggaa attaggtcgt cggctgtcta ctcagcaggt tgcggatgac cttcaaaatc
     3901 tcttcggcaa tcaaccgatg tccctccgcg gtggggtggc agtggtcgat aaatagcttt
     3961 tctccgcctt cggcctggaa ggtcgccggt aggtcgataa cgatgagatt cttctctcct
     4021 ttcaacctgt cgattttctc ccagtacgtt ctcttaactc gcaaagagaa ataatccgcc
     4081 tgaagggcgg aatcgaggta ttgaaacgcc gcgcgggccg tgtcgtgcag gagattgggg
     4141 ccatagtttt ctaacgataa gagattaatg ccgatgttgt agagaaaagg agccccggcc
     4201 gattccacgg tgggattgag actgtcgcga tagtggcgga ggtataagtt tcgagctgtc
     4261 ttcaagtaat aatcagcctc ccagtaccgc cggtcttccc agagcaatac ccccaggttg
     4321 ttgaaaagca ccggatttcc ggagtctgtg gcgattaagt tcgcgtagta agctatagcc
     4381 tcctgcggtg gcagagaatc gctataggcc gaccgataga cgctgcgggt aaaaatatcg
     4441 ccccgaccgt ataaagcggt gaagcgttcc gggctcaggc agtacttgat tggtcttccc
     4501 agaattctct gcattgcatc agggataatc aggcggccgt cttctccgga gacctgtgta
     4561 aaaagtttga actgcagtcc ggccggccag aggtagggta ccggcggttt gagaacgatg
     4621 agcgggcagc cgtgctggcg gcactggtcg gcgatgcggg tgagattcgt cccgaaccgg
     4681 cttgcgggaa ctctgacttg tagcgtttcc gccggtcggg taccgctcca cctatcctgc
     4741 accaggagag cccgtaaaac ccggtacagc gccagatggg agaagagcct ccggactttc
     4801 cgtaatttct gctgtcccag taattgccga tccgagtagc ttccggatat cgaagcatcg
     4861 ttatttccgc agtacagtat gaccagatca ggtttgaagt cccacccgcg gagttccaga
     4921 aatcgggcga tctgttcgct ggtatagcct gaaacagcgg cattgatcaa ttcaaaacgc
     4981 ttcctatcag catattcacg ttcgaggagc gactccaaca gttctccgaa agcgagcgag
     5041 tgggattgca gtcccagtcc caccggggat gagtccccca agagcagcag gcgataggtc
     5101 cg
//
LOCUS       DBNGZA010000140         1007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2570145, whole genome shotgun sequence.
ACCESSION   DBNGZA010000140 DBNGZA010000000
VERSION     DBNGZA010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2570145"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /locus_tag="AC16FU_03070"
     CDS             <1..>1007
                     /locus_tag="AC16FU_03070"
                     /inference="COORDINATES: protein motif:HMM:NF040057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR2993389.1"
                     /translation="ITEDEIALVGSGSVQYQNLTLSADSIHYHTETQILNAGGLALTP
                     PGEKRYRLQDGSDEIFFSRLSYNIRTRKGRLKQSSTQLEGGYYHGSNLAKVDENVLLV
                     RQGRYTTCDDPNPHYYFGSRNMKIVAKDKVVAKPVFFYVAGIPLFGVPYYLFPIKPGR
                     HSGFLTFRIGDFAQGRRGVRDLGYYWAFSDYWDLESAVDLTEGGGNLSLLWRNRINYA
                     WRYHLSGSVSGSYNRSSSGFGAAKTNRWDLTLNHFQELSPTARLTAAGSFVSDKSYYP
                     IFANTLNQRLNRVLNPRASLTKSFGSGVSVFAAGEFVRNLDTDARTLTLPTLSITKPQ
                     RP"
BASE COUNT          198 a          321 c          283 g          205 t
ORIGIN      
        1 atcaccgagg atgaaatcgc tttagtcggc tccgggagcg tgcagtatca gaacctcacg
       61 ctcagcgccg attcgattca ctatcacacc gaaacgcaaa ttctcaacgc cggcgggctg
      121 gcgctgacgc cgccgggaga aaagcgctac cggcttcagg acggctccga tgagattttc
      181 ttttcgcgcc tgtcgtacaa tatccgcacc cgcaaaggcc ggctcaagca gtcgtccacg
      241 caactggaag gcggctacta ccacggctcg aacttagcca aagtcgacga gaacgttctc
      301 ctggtgcggc aggggcggta caccacctgc gacgacccga atccgcacta ctacttcggg
      361 agccggaata tgaaaatcgt ggccaaagat aaagtggtgg ccaaaccggt cttcttctac
      421 gtggcgggca ttccgctctt cggggtgccc tactatcttt ttcccattaa gcccggccgg
      481 cactccgggt ttctgacctt tcgcatcggt gattttgccc agggccggcg gggcgtgcgg
      541 gatttgggct actactgggc tttttccgac tactgggatt tggagagcgc ggtcgatctc
      601 accgagggcg gcgggaacct ttctttactc tggcggaacc ggatcaacta tgcctggcgc
      661 tatcacctgt ccggttcggt ttccggttcg tacaaccgct ccagtagcgg cttcggcgcc
      721 gcaaaaacca atcgctggga cctcacctta aaccactttc aggaactgtc gcccacggcc
      781 cgcctgaccg ccgccggcag tttcgtgagc gacaagagct attacccgat cttcgccaat
      841 acgctcaacc aaaggctgaa ccgggtgctc aacccgcggg ccagtctgac gaagtcgttc
      901 gggagcggcg tgagcgtctt tgccgccggc gagttcgtcc gcaacctgga caccgatgcg
      961 cggacgttga cgttgcccac cctttcgatc accaagcccc agcggcc
//
LOCUS       DBNGZA010000141         4884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1721940, whole genome shotgun sequence.
ACCESSION   DBNGZA010000141 DBNGZA010000000
VERSION     DBNGZA010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1721940"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..617)
                     /locus_tag="AC16FU_03075"
     CDS             complement(30..617)
                     /locus_tag="AC16FU_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR2993390.1"
                     /translation="MTTARIETGFLGQAFILPRIAVNAAGIVFFAIATAIGAQIRIPL
                     PFTPVPVTGQTFFVLLSGLTLGSRLGGTTQLLYLLLGITGLPFFSSGKGGWSILAGPT
                     GGYLVGFVLAAWLAGYFPRPRKNLFTSIGALWFVSLSILSLGTLWLGVSLHLSPAAAI
                     QQGFLPFLVGDALKVLAASGLFRLYHRHAKKLFPR"
     gene            complement(671..2404)
                     /locus_tag="AC16FU_03080"
     CDS             complement(671..2404)
                     /locus_tag="AC16FU_03080"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR2993391.1"
                     /translation="MALRESPADLRLDGEPVVISNIEIAHRAKLNPIRNIMDDLGITD
                     DDFEFYGKFTGKIRLETLAKFSARPDGKLIVVTAMTPTSKGEGKTLTTVGLGQALAKI
                     GKKGAITLREPSLGPVFGVKGGATGGGYAQVIPMEHINLHFNGDIHAVTAAHNLLAAM
                     VDNHILKGNDLRIDVTNILWNRALDMNDRSLRQIVVGLGGRVNGVPRESGFVITAASE
                     VMAILALAQSRADLKARVGEIVVGYTLEGTIVKAKDLQAHNAMAVMLSEAIMPNLVQT
                     IEHTPALIHAGPFANIAHGTNSVMADKIALKLADYVVTECGFGADLGAEKFFDIVCRS
                     SGLWPSAAVVVATCRGIKYHGGVPEKPESLLTQENLEASQKGLDNLAAQIRNVRRFGV
                     PVIVAVNRFPFDTPAELGMIFDLCQKLEVDCATNEAFAKGGNGAKELAEKTVALADDH
                     RDPEPTFLYDLTLSVEEKVEKIATQMYGAAGIYFEKRAKKKIEKFVELGYGNLPVCIA
                     KTQSSLSDNPRARGVPKGWTLTVSDAFLSAGAGYLVLACGEMMLMPGLPKIPAAVQMD
                     VDEHGNITGLF"
     gene            complement(2477..3958)
                     /gene="betB"
                     /locus_tag="AC16FU_03085"
     CDS             complement(2477..3958)
                     /gene="betB"
                     /locus_tag="AC16FU_03085"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008272605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HIR2993392.1"
                     /translation="MTTITEVKTEPKVRPGRLFIGGKWIEAASGKTFETINPANGEPL
                     AAVAEGDALDIDFAVKAARKAFESDPWASMSAADRGKIIWKIGDLILKYADEFARLES
                     LDNGKPIWESKNIDLPQTADVFQYYAGWATKIHGETVPVKGNYFAYTLREPVGVVGGI
                     IPWNFPLLLASWKVAPALACGNTMVLKPAEQTPLTALRLAEICQEAGLPDGVLNVVTG
                     FGPTAGAALVKHPDVDKIAFTGSYQVGQEIMRNAASTLKKISLELGGKSPNIVFADAD
                     LDAAVKGATVGIFYNKGEVCCAGSRLFVEEKIHDQFLEKLLERAKKLVPGDPLDPKTR
                     LGPLVSDEQLRKVTSYIEAGKREGAKLVCGGDRPAVGNGKGYFLNPTVFDDVKNEMKI
                     AREEIFGPVLATIRFADFDEVIAKSNDTFYGLAAAIWTKEIKKAHTAARALKAGTVWI
                     NTINMFDSTVPFGGYKMSGFGRELGQHALELYTQVKSVWVDLN"
     gene            complement(4006..4455)
                     /locus_tag="AC16FU_03090"
     CDS             complement(4006..4455)
                     /locus_tag="AC16FU_03090"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2993393.1"
                     /translation="MRPEEVSTVAQLQLEMWQDHAQNPIYQVVPDALELLQNNLREHL
                     NHPDWRIFVADVDGDPVGTLSCWIIEQKFLFVPRRFGYIGSVYVKPAFRRRGLTRQLT
                     ELAIQFCRAQSVTQVRLNTDVSNPVSNATWQALGFKPAAYTLYLDLM"
     gene            complement(4583..>4884)
                     /locus_tag="AC16FU_03095"
     CDS             complement(4583..>4884)
                     /locus_tag="AC16FU_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2993394.1"
                     /translation="TGQLFSFEEAEKLGLVNHVFPADKFWEDGLKFAQQFVPPAKASR
                     AVGRIKRAVCSGVEVPFSEGLAIERELQQQLFMSEDAKEGLNAYVEKRPAVFAGR"
BASE COUNT          991 a         1413 c         1410 g         1070 t
ORIGIN      
        1 gagctcacag gggctgagct cttttcatct tagcgcggga aaagtttttt agcgtggcgg
       61 tggtaaaggc gaaacaaacc ggaagcggca aggactttca gcgcgtcacc aacgagaaaa
      121 ggcaagaaac cctgctgaat tgcggcggca ggcgacaagt gcaaggatac gcccaaccac
      181 aaagtgccaa gagagagaat tgaaagggaa acgaaccaga gtgctccgat gctcgtgaaa
      241 agatttttcc gggggcgggg gaaatacccg gccagccagg cggctaggac aaagccgacg
      301 agataccccc ccgtcggtcc ggccagaatg ctccagccgc ctttgccact ggagaaaaag
      361 ggcagcccgg taattcccag aaggaggtaa agcaattgcg tcgttccgcc gaggcggctc
      421 cccagggtta agccagagag cagaacgaaa aaggtttgtc cggtgacggg gaccggtgta
      481 aagggaagcg gaatccgtat ctgtgcgcca atggccgttg cgatggcaaa gaaaacgata
      541 ccggcggcat tcaccgcgat gcggggcaga ataaaagcct gtcctaaaaa tccggtttcg
      601 attctggcag ttgtcactct tgtcacccct ggttggttcg acgcattctg tctcggaaga
      661 tcatgccggt tcaaaacaga ccggtgatgt tgccgtgttc gtccacatcc atctgaactg
      721 cggccggaat tttgggcagc ccgggcatga gcatcatttc gccgcatgcc agaacgagat
      781 acccggcgcc ggcggaaaga aacgcgtcgc tcacggtcaa agtccaaccc ttgggcacgc
      841 cccgcgcgcg gggattgtcc gaaagggagg actgggtttt ggcgatgcaa accggcagat
      901 tgccgtagcc cagttcgacg aatttttcga ttttcttctt ggcgcgcttt tcgaaataga
      961 tcccggcggc gccgtacatc tgggtggcga ttttctccac cttctcttcg acggacaggg
     1021 taaggtcgta gaggaaagtt ggttccgggt cgcgatggtc atcggctaag gcgaccgtct
     1081 tctccgccaa ctctttggcg ccgttgccgc ctttggcaaa cgcttcgttc gtagcgcagt
     1141 ccacttccag tttctggcag aggtcgaaga tcatgcccaa ctcagccggc gtatcgaatg
     1201 ggaacctgtt cacggccacg atcaccggca ccccgaaccg gcgcacgttg cgaatctggg
     1261 cggcaagatt gtccagtcct ttttgggacg cttctaagtt ttcctgcgtc aaaagcgatt
     1321 ccggtttctc cgggacgccg ccgtggtact tgattccccg gcaggtcgcc accaccaccg
     1381 ccgcagaggg ccacagtccg gatgagcggc agacgatgtc gaaaaatttc tccgcgccga
     1441 ggtcggcgcc gaagccgcat tcggtgacga cgtagtcggc cagtttgagg gcgatcttat
     1501 ccgccatgac gctgttggtg ccgtgggcga tattggcgaa ggggccggcg tggatcaagg
     1561 cgggggtgtg ctcgatcgtt tgtacgaggt tgggcataat cgcttcgctg agcatgacag
     1621 ccatcgcgtt gtgggcctgg agatctttcg ccttgacgat tgttccctcg agagtgtagc
     1681 ccacgacaat ttccccgacg cgggctttca aatcggcccg cgattgagcc aaagccagga
     1741 tcgccataac ttcagatgcg gcggtgatga caaagcccga ttcccggggc acgccgttca
     1801 cccggccgcc caggccaacc acaatttgcc gcagggagcg gtcgttcata tcgagcgccc
     1861 ggttccagag aatgttggta acgtcgatgc gcaggtcgtt gcccttgagg atgtggttgt
     1921 ccaccatcgc tgctaataag ttgtgcgctg cggtgacggc atggatgtcg ccgttgaagt
     1981 gcaggttgat gtgctccatc ggaatcacct gcgcatagcc gccgccggtg gcgccgccct
     2041 ttacgccaaa gaccggaccc agagagggct cgcgcagggt gatggcacct tttttcccaa
     2101 tcttcgccag ggcctgaccc aggcccaccg tcgttaaggt cttgccttcg cccttgctcg
     2161 tgggggtcat ggccgtgacc acgatcaatt tcccatccgg acgggcggaa aactttgcga
     2221 gcgtttccag acggattttg ccggtgaact ttccgtagaa ttcgaaatcg tcatcagtaa
     2281 tgcccaggtc atccataatg ttgcggatgg ggttcagttt cgcccggtgg gcgatctcga
     2341 tattggagat gacaaccggt tcaccgtcca gccgcaggtc ggccggactc tctcttagag
     2401 ccattcggtt cggttccacg gaaaaactcc tcacctttcg ggagcgcagg gagagcccgg
     2461 gcgcttccgc agagtatcag ttcaaatcca cccagacact tttgacctgg gtgtagagct
     2521 ccagagcgtg ctgacccaat tcccggccaa agcccgacat tttgtatcca ccgaaaggta
     2581 cggtgctgtc gaacatgttg atggtattga tccagaccgt gccggctttg agcgcgcggg
     2641 ccgccgtgtg ggctttcttg atctctttcg tccagattgc ggcagccaga ccgtagaagg
     2701 tatcgttact cttggcgatc acttcgtcaa aatcggcaaa gcggatggtg gctaatacgg
     2761 gaccaaaaat ctcctcgcgg gcaatcttca tttcattctt cacgtcgtcg aaaaccgtgg
     2821 gattcaaaaa atatcccttg ccgttgccga ctgcgggccg gtcgccgccg cagaccagtt
     2881 tcgccccctc ccgcttaccc gcctcgatgt aggaggtcac cttgcgcaac tgttcgtcgg
     2941 aaactaaagg ccccaggcgc gttttggggt cgaggggatc acccgggaca agtttcttgg
     3001 cacgttccaa gagtttttct aaaaactggt catggatttt ttcctctaca aacaggcgcg
     3061 agccggcgca gcaaacttcc cctttgttgt agaaaatccc gaccgtggcg cccttgaccg
     3121 ccgcatccag atcagcgtcg gcgaagacga tgttgggtga tttcccaccc agttcgagcg
     3181 aaatcttctt caaggtgctg gcggcattgc gcataatttc ctgccccacc tgatatgagc
     3241 cggtaaaagc gattttgtct acgtccggat gtttgaccag cgccgcgccg gcggtgggtc
     3301 caaagccagt caccacgttc agcacgccat cgggcaagcc ggcttcctga cagatttcgg
     3361 ccaagcgcag ggcggtcaaa ggagtctgtt cggcgggctt cagaaccatc gtattgccgc
     3421 aggctagggc gggcgcaacc ttccaggacg ccagaagcag agggaaattc cacgggataa
     3481 tgccgcctac tacacccacc ggctcgcgca gggtataagc gaagtagttt cccttcaccg
     3541 gcaccgtctc gccatggatt ttggtggccc agccggcata gtactgaaac acgtcggcgg
     3601 tctgggggag atcgatgttt ttcgattccc agatcggttt gccgttatcc agcgattcca
     3661 gccgggcaaa ctcgtccgca tatttcaaaa tcaagtcgcc aattttccat atgattttcc
     3721 cccggtcggc ggcactcatg ctcgcccagg ggtcggactc aaaggccttc cgcgcggcct
     3781 tgactgcaaa atcgatatcc agcgcgtcgc cttcagccac cgcagccagg ggctcgccgt
     3841 tggccggatt gattgtctca aaggtctttc ccgaggccgc ctcgatccat ttgccgccaa
     3901 tgaaaaggcg ccccggacgg acttttggtt cggtctttac ttcggttata gtagtcatct
     3961 cgttcttctt ctcccttgcg ggactgaagt cccgcctccc gggatttaca tcaaatccag
     4021 atacagcgtg taagccgccg gtttgaatcc cagagcctgc caggttgcgt tggagaccgg
     4081 gttggacacg tcggtgttca gccgcacttg agtgacactc tgtgcccggc aaaattgaat
     4141 tgcgagctcc gtcagttgtc gcgtaaggcc ccggcggcgg aaggccggtt ttacgtacac
     4201 gctcccgatg taaccgaaac gacgcgggac gaagaggaat ttctgctcga tgatccagca
     4261 ggagagcgtg ccaaccgggt cgccatccac gtcggcaacg aaaattcgcc agtccgggtg
     4321 attgaggtgc tcgcggaggt tgttctggag tagctcaagt gcatcgggca ccacctggta
     4381 gatcgggttt tgcgcgtgat cctgccacat ctcaagttgc aactgcgcca ccgtagaaac
     4441 ttcctctggc cgcatgagcc ggattaccgg ttttttcttt ccggtttgtt tctgaaagat
     4501 ttcagccgtt gtttcaaccg tgattcactt gaccgggttt gtgttttctg gtccctggct
     4561 accggcaact ggctactttt tttcaccggc ccgcaaaaac cgccgggcgc ttctcgacgt
     4621 aggcgttcag tccttctttg gcgtcttcgc tcatgaacaa ctgctgctgc aactcgcgct
     4681 caatggccag gccttcggag aagggcacct ccactccgga gcagaccgcc cgcttgatcc
     4741 gccccaccgc ccgggaggcc ttggccggcg gcacgaactg ctgggcaaac ttgagaccat
     4801 cctcccagaa cttgtccgcc ggaaagacgt gattcaccaa tccgagtttc tcggcttcct
     4861 caaaggagaa gagttgcccg gtcg
//
LOCUS       DBNGZA010000142         3569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_663105, whole genome shotgun sequence.
ACCESSION   DBNGZA010000142 DBNGZA010000000
VERSION     DBNGZA010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_663105"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="AC16FU_03100"
     CDS             complement(<1..971)
                     /locus_tag="AC16FU_03100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR2993395.1"
                     /translation="MPRFVKNLLLAIASVLIFFGSIEVVLSLLHVEEKFQKRTFLLEP
                     ERDYPQFFAKDRNLFWKFRPNQTIQSHFFVEGEYRINSHGFRDREFSVAKPPGTFRIV
                     CLGNSCTFGWGVRQEETYPKVLEKILSDSLPGRKVEVINAGVTGYSSLQGLRLFEREI
                     LKLQPDLVTVCYGWNDHWPAVGGIPDTNQRLPAQWVLDLENFFDRLQTYRLLRRALGG
                     VSLNKQRYQDKSGTTRLRATPEEVEANLRELAFQAKARSIPLILVTPPSASPAWPWNE
                     IDYKRFTRIWQVHQQYTNAVRMTAQRWQVPLVDADFDFQQSIEYYDNP"
     gene            complement(984..1163)
                     /locus_tag="AC16FU_03105"
     CDS             complement(984..1163)
                     /locus_tag="AC16FU_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR2993396.1"
                     /translation="MVKVGGVKEKVSLARELWAFMRTRKKWWLGPILFILLLMAALII
                     FAETSAIAPFIYTLF"
     gene            complement(1189..2928)
                     /locus_tag="AC16FU_03110"
     CDS             complement(1189..2928)
                     /locus_tag="AC16FU_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR2993397.1"
                     /translation="MHILGISAFYHDAAACLLKDGEVVAAAQEERFSRIKHDFNFPIK
                     AIQYCLKEAGITSKDLSYVGFYEKPLLKFERILYQYFSTFPRSFNSFRQALPIWLKEK
                     LWIPGLIRKKLDYEGEILFIEHHLSHAASTFLVSPFKEAAILTIDGVGEWATATYGIG
                     RDNDITIFKEIRFPHSLGLLYSSFTYYLGFRVNSAEYKVMGLAPYGEPKYVDLIKREI
                     IDIKEDGSFVLNQDYFAYTYGLTMTNGKFDHLFDGPPRESDDPLEQRHKDLAASVQKI
                     TEEVMLKMANHLHREAKTKNLCLAGGVALNCVANGRILRETPFAELFIQPAASDAGGA
                     VGVATYIWHTLLKKERRWTWPHAFWGPEYSDEEIRSFLESQKVPFHRYERRKLLDHTA
                     QLIDEQKVVGWFQGRMEFGPRALGSRSILADARNPRNRDVVNLKIKFRESFRPFAPAV
                     LAEKASDYFELSCPSPYMLLTAQVRPDRRTIPAVTHVDGSARVQTVAREDNSRFYDLL
                     AAFAQRTGCPVLINTSFNVRGEPIVSSPEEAFRGFVNTDMDVLVIGSFVLLKVEMPPG
                     LRERGWYKKFQPD"
BASE COUNT          826 a          980 c          987 g          776 t
ORIGIN      
        1 ggattatcgt agtattcgat tgactgttgg aaatcgaagt cggcatcaac cagcggcacc
       61 tgccacctct gggccgtcat ccggacggcg ttggtgtact gctggtgcac ctgccagatt
      121 cgggtgaagc gcttgtagtc aatctcgttc cagggccagg cgggcgaagc ggagggcggc
      181 gtcacgagaa tcagcggtat gctccgcgct tttgcctgga acgccagttc gcgcaggttc
      241 gcttccacct cctccggagt ggcccgcagc cgcgtcgtcc cgctcttatc ctgatagcgt
      301 tgtttattca gggaaacgcc gcccagtgcg cgcctgagca agcggtaagt ctgcagccga
      361 tcaaagaaat tctcgagatc cagtacccac tgagccggga gacgttgatt ggtatcggga
      421 atccccccca ccgccggcca gtggtcgttc cagccgtagc agacggtcac gaggtccggc
      481 tggagtttga gaatctcccg ctcaaagagc cgtaacccct ggagcgacga gtaaccggtg
      541 actcccgcat tgatgacctc aaccttccgc cccggaagcg aatcagagag tattttctcc
      601 agtaccttgg gataagtctc ctcctgccgc acgccccagc cgaaggtgca agagtttccg
      661 aggcagacga tgcgaaacgt tcccggtggt ttggcgaccg aaaactcccg atccctgaag
      721 ccgtgcgaat tgattcggta ctctccctcg acgaagaagt ggctctggat ggtctgattc
      781 ggacggaatt tccagaatag gttgcggtct ttggcaaaaa actgggggta gtcccgttcg
      841 ggttcaagga ggaaagtgcg cttttgaaac ttctcctcga cgtggaggag actgagcacc
      901 acttcgatgc tgccgaagaa aatcagaaca ctcgcaattg ccaggagaag atttttgaca
      961 aagcgcggca tcgtggcggg aggttaaaac aaggtgtaga tgaagggcgc aatggccgag
     1021 gtctcggcga agataatgag cgccgccatc aggagcaaaa tgaaaagaat cggccccaac
     1081 caccacttct tacgcgtgcg cataaaggcc cacagctcac gcgccagcga tactttttct
     1141 tttacaccgc cgactttaac cattcctcct catcgctgcc gaccaatctc aatcgggttg
     1201 gaatttctta taccagccgc gttcgcgcaa gcccggcggc atctcaactt tgagcaaaac
     1261 gaaggaaccg atgaccagaa catccatatc ggtattgaca aaaccccgga aggcctcctc
     1321 cggcgaagag acgatcggct ccccgcgcac gttgaacgag gtgttgatga gcaccggaca
     1381 cccggtgcgc tgcgcgaacg ccgccagaag gtcgtagaag cgcgaattat cctcgcgggc
     1441 aaccgtctgc acccgggccg agccgtccac gtgcgtcacc gccggaatcg tgcgccggtc
     1501 ggggcgcacc tgcgcggtca ggagcatata ggggctgggg caactgagtt cgaagtaatc
     1561 ggacgctttc tcggccaaca ccgccggcgc aaagggccgg aacgattccc ggaatttgat
     1621 tttgaggttg accacgtccc ggttccttgg attgcgcgca tcggccagaa tggagcggct
     1681 gcccaaggcg cgcgggccga attccatccg cccctggaac caccccacca ctttctgctc
     1741 gtcaatcaat tgcgccgtgt gatcgagtaa cttccgccgc tcgtagcggt gaaagggtac
     1801 cttctgcgat tccaaaaacg agcggatttc ttcgtcggaa tactccggcc cccagaaagc
     1861 gtgcggccag gtccaccggc gttctttttt caagagggta tgccagatat aagttgcgac
     1921 ccccaccgcg ccgccggcgt cgcttgccgc gggctggatg aatagttcgg cgaaaggcgt
     1981 ctcgcgcaga atccgtccgt tggccacgca gttgagcgcc accccaccgg ccagacagag
     2041 gtttttggtt ttcgcttcgc ggtggaggtg gttggccatc ttgagcatca cctcttcggt
     2101 gattttctgg acgctggccg ccaggtcttt gtgcctttgt tccagaggat cgtcggattc
     2161 acggggcggc ccatcaaaaa ggtggtcgaa tttgccgttg gtcatggtca agccgtaggt
     2221 gtaggcgaag taatcctggt tcagcacgaa cgagccgtcc tctttgatgt cgatgatctc
     2281 gcgcttaatg aggtctacgt acttcggctc accgtaaggc gccaggccca tcaccttata
     2341 ctccgcgctg ttcactctaa aacctaaata gtatgtgaaa gagctgtaga ggaggcccag
     2401 agagtgcggg aagcgaattt ctttgaaaat cgtgatgtca ttatcccgac cgatcccgta
     2461 ggtagccgtc gcccactctc ccacgccgtc gatggtcaga attgccgctt ccttgaaagg
     2521 cgaaaccaga aaagtggacg ccgcgtgcga caggtggtgc tcgatgaaaa gaatttcgcc
     2581 ctcgtagtcg agttttttgc ggatcaagcc gggaatccag agtttttcct tgagccagat
     2641 gggcagcgcc tgccggaaag agttgaacga gcggggaaaa gtcgagaagt attgatagag
     2701 gatgcgctcg aatttcaaaa ggggcttttc gtagaaaccg acgtaagata ggtctttgct
     2761 tgttatcccc gcttctttca gacagtactg gatggccttg atgggaaagt taaagtcatg
     2821 cttgatgcgg gaaaaccttt cctcctgcgc cgccgccacg acttcaccgt ctttgagcag
     2881 gcaggccgcc gcatcgtgat aaaaggccga aatgccgaga atatgcatag caaaaatata
     2941 acaccctttc cccgctgcgt gtaccccctt cgttcgcgga aatgttccta agaaacgtag
     3001 gggtgaatcc gcctcaggcg gattcacccg ctctgcttgt aattaaaaca ttcttttcgc
     3061 cctttcccag gacacctcca tcggctcttt ggacacccag aaggtggggc gattgcgcct
     3121 gcggacgttc agaaggtctt tgcggaggag aaaagagacc agccaaacga ggccaactac
     3181 aagaaagtaa aagagagaga caatgattgt ggtctggatt ttgcccaggc ggtgggcgaa
     3241 ggatttccaa acttgccaag cctttctaaa agtggttttt gcgctatccc ccattgccga
     3301 aggccctcat cttaaggaaa caaatttgac tgagaaatta cacttgtaac atcgaatatc
     3361 tcgattaaat cgtcgaaaac caaaaaaatt acatccagga cactgatttt tcaaaaacca
     3421 gtcaccagcg ttgggcagat atgtagaaaa cctgactgaa agaggtagta ggacctcctt
     3481 cgcaactcgg taacgatctt tggctgtaag tttttcaaaa agcagagaaa tgagttctcc
     3541 aagtgactgc gtccatgtat ttcctgcct
//
LOCUS       DBNGZA010000143         7330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1938061, whole genome shotgun sequence.
ACCESSION   DBNGZA010000143 DBNGZA010000000
VERSION     DBNGZA010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1938061"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC16FU_03115"
     CDS             complement(<1..828)
                     /locus_tag="AC16FU_03115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR2993398.1"
                     /translation="MAYHELAVRLPAKYHDAVSNFLITRGVNGLITKDGSHWLIGYAF
                     DRQSLAEKKGALLAYLRSLQKLNHPFPFRITTREIPRWDWSQRWKESFAPKELSCGIV
                     IKPPWVRRAFPGKLVITINPKMAFGTGEHATTQLCLSAVYALTRPGMRVLDVGTGSGI
                     LAIAAVKLGAKTAFGCDTDPLAIDNARENSRLNGVAKKIQLACGSLERTPAEKTYNLV
                     LANLITNQVIAYLPDLVRRARPRGYLILSGVLARQKRRVLDAMEKQKLHLVSIGQQHE
                     "
     gene            complement(832..2004)
                     /gene="dnaJ"
                     /locus_tag="AC16FU_03120"
     CDS             complement(832..2004)
                     /gene="dnaJ"
                     /locus_tag="AC16FU_03120"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR2993399.1"
                     /translation="MAKRDYYEVLSVERGASEDEIKKAYRKLALQHHPDRNPGSKEAE
                     EKFKEATEAYEVLKDPEKRRRYDQFGHVGVGAGAGAGAGGFSDFGFGGFDLSDALRAF
                     MRDFGGFEEFFGAGTPRRSRGERGGATRGRDLQVRLQLTLEEIANGAEKTIKLRRLVK
                     CETCNGSGAEKGSSKRTCPTCRGSGEIRQVSRSLFGQFVSVSTCTTCGGEGVVVERPC
                     AACGGEGRVRGQSTINVKVPAGVATGNYIPIRGAGDVGPKGGPAGDVYVLIEEEEHPV
                     FTRHENNVICEIPISMIQAALGDEIEVPTLDGRAQIKIPAGTQSGKIFRLKNKGIPHL
                     HAHGKGDELVRVVVWTPTSLSSEDKRLLRELEKSENLRPPKADKSFFTRLRESLGF"
     gene            complement(2362..3150)
                     /locus_tag="AC16FU_03125"
     CDS             complement(2362..3150)
                     /locus_tag="AC16FU_03125"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR2993400.1"
                     /translation="MKQGVKLFVLLALSTLFISVATIATAGDTKNGEAKKTYWKITGQ
                     LEEACSCNPACPCWFDSKPTRMTCGGGQVLFIEEGTYGDVPLKGLAVANMVESPPGQS
                     MMESFGKWKFSYLYIDEKASPEQRQALEELGKTVLSSHASSPNVKIRYVPIVRAIEGK
                     EHKITIGDYGSFHGHLIEGGLGGTPKIVNPPGADPIHQEYQQGQTTKLTYSDAEQNWN
                     YEKSNYMFTTFTVDSDQYEKYAAGLAQKMKAMKKQEGTEKGNRK"
     gene            complement(3359..3991)
                     /gene="grpE"
                     /locus_tag="AC16FU_03130"
     CDS             complement(3359..3991)
                     /gene="grpE"
                     /locus_tag="AC16FU_03130"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR2993401.1"
                     /translation="MSKKKEKGEEKVTVRNTVPLPSAQEAGAPPRRVESTTEAVPTLE
                     ETLRNQLDESETRFKELEDRYLRLAAEFDNFRKRTNRDLATIIKNANENLILQQIDIV
                     DNFHRALDAAKTSADFDSFHKGVELIYTQMFDILNREGVQIIEPAGQVFDPHFHEAIM
                     YVESDQPEGKVIEVVSTGYLLNDKVLRPARVVVSKGKTRASPENREEEKS"
     gene            complement(4063..5097)
                     /gene="hrcA"
                     /locus_tag="AC16FU_03135"
     CDS             complement(4063..5097)
                     /gene="hrcA"
                     /locus_tag="AC16FU_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR2993402.1"
                     /translation="MTKPFENLNEREREILHALIDHYIRTADPVGSRILATRYPFGLS
                     PATIRNTMQDLESRGLVEQPHMSAGRVPTDAGYRLYVDNLLRPEQLTEEEKERIRNEV
                     VLDQVAVDRILEQTSRVLAGVSHQLGVTIAPSFEQGVLTRIDLVPVTERKLLVIIAVK
                     SGLAKSVLLEVDVAIDERLIGETARLLNERLAGLTLGEIKRTIRERLSAPGEGDPKLL
                     RLFLNASDQLLSAEEAEHLHIEGTANVVNQPEFKDHNRLTTLFSLLEERKSIVEMLNT
                     QGIKEGIVITIGSENPRAEGQNLSVVTSTYQAGKVKGTIGVIGPTRMPYSKLVSVVDY
                     TAKVLSEIFS"
     gene            complement(5166..6407)
                     /locus_tag="AC16FU_03140"
     CDS             complement(5166..6407)
                     /locus_tag="AC16FU_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR2993403.1"
                     /translation="MRLRHRLRRRFQTFLIFLAVLGPGMITSNVDNDAGGITTYSLAG
                     AQFGYNLLWVMIPVVVALIVIQEMVARMGVVTGKGLADLIRERFGVRTTVYLLLAIVI
                     TNYANTVSEFAGIAASLEIFNISRHLSVPVGAILVWLLVVKGNYKTVEKVFLVACLVY
                     FTYIVSALMARPDWSEVLRAVKATDISFDSKSLAMVIGLVGTTIAPWMQFYLQSAIVE
                     KKIHVNHLKHSRWDVISGSVIVNVVAFFIIVACGATLFVKGVQIETAKEAALALAPLA
                     GKYASYLFAFGLLNASLFSASILPLSTAYMVCEGMGWQDGVDKKFSEAPLFYLLYTLL
                     ILGGAVTVLLPGIPLIKIMYFSQVLNGIVLPLILVFMLLLVNDRRLMGKYVNGPVFNA
                     IAIVTAGTLIVLSILLVSFVR"
     gene            complement(6411..>7330)
                     /locus_tag="AC16FU_03145"
     CDS             complement(6411..>7330)
                     /locus_tag="AC16FU_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR2993404.1"
                     /translation="KRLMEAGIILHTQVVLCPGLNDGAFLEKTIRDLAHFYPKIPSLA
                     VVPVGLTQYRARLTPMHSVDRDYAREVITYLDSRQKWLKEKLGTHFVYPADEFFLLAD
                     WLVPPADYYDDFPQVENGIGMVRRFLDDFQARKSKLPKRLTKPVKILIVTGTSAGKVF
                     RSEIIPELRYFSGLQCDLLIVENKFYGPSVTVSGLLTAGDIVDAIKEHGQKYNYVLLP
                     PSCLNTDGIFLDDLTLEDVAARCGMPVVRGQISLVDTLLPYLNRKRSKSRVNPKPTNS
                     HGGLFHEYQVESEPSPSEEEALFRNRTIA"
BASE COUNT         1528 a         2039 c         2120 g         1643 t
ORIGIN      
        1 ttcatgctgt tggccgatag acaccaaatg caacttctgc ttttccattg cgtccagtac
       61 ccggcgcttt tggcgggcaa gcactcccga aagaatcaaa tagccccgcg gtcgggcacg
      121 gcgaaccaaa tcgggcaggt atgcgatcac ctgattggta atcaggtttg ccagaaccag
      181 gttgtacgtt ttttctgcgg gggtacgttc gagtgagccg catgccagtt gtattttttt
      241 tgcgactccg ttcagacggg agttctcccg ggcattgtca atagcgagcg ggtcggtatc
      301 gcagccgaaa gcagttttag ctcccagttt cacggcggca atcgccagaa tgccgctgcc
      361 ggtgccgaca tccagaactc gcattccggg acgcgtcaac gcatacaccg cggagagaca
      421 caattgtgtg gtggcatgct cgccggtacc gaaggccatc ttgggattga tggtgatgac
      481 cagttttccc ggaaaggcgc gcctgaccca gggcggtttg atgacgatgc cgcaggataa
      541 ctctttcggc gcaaaacttt ccttccacct ctgcgaccaa tcccagcgcg gaatttcccg
      601 ggtggtgatg cggaagggaa acgggtgatt cagtttctgg agacttcgaa gatatgcaag
      661 taaggcgcct tttttctcgg cgagactttg gcgatcaaaa gcgtaaccga tgagccagtg
      721 ggaaccatcc tttgttatca aaccattgac gccgcgggtg atgaggaaat tgctgacggc
      781 gtcgtggtat ttggccggca gacgcaccgc cagttcatgg taggccatgc cttaaaaacc
      841 cagactttcc ctgagacggg taaagaagga cttgtcggct ttggggggcc tgagattctc
      901 gcttttctcc agttcgcgca ggagccgttt gtcctcggac gaaagggagg tcggcgtcca
      961 gaccaccacc cgcaccagtt cgtcgccttt gccgtgtgcg tgaagatgcg gaatcccttt
     1021 gtttttcaag cggaagattt tcccgctctg cgtcccggcg ggaattttga tttgcgcccg
     1081 gccatccaga gtcggcactt cgatttcatc ccccagcgct gcctgaatca tgctgatcgg
     1141 aatctcgcag atgacgttat tctcgtggcg ggtgaagacg ggatgctctt cctcttcgat
     1201 taagacgtag acgtcgcccg ccgggccgcc tttggggccg acatcgcccg cgccgcgaat
     1261 cgggatatag ttgccggtgg cgactcccgc cgggactttg acgttgatgg tcgactgccc
     1321 gcgcacccgg ccctctcccc cgcaggcggc acagggacgt tcgaccacga ccccttcccc
     1381 gccgcaggtg gtgcaagtgg aaacgcttac gaactggccg aagagcgagc ggcttacctg
     1441 ccggatttcg ccggagccgc ggcacgtggg acaggtgcgc ttggacgatc ctttctcggc
     1501 gccgctgccg ttgcaggtct cgcactttac cagccgccgg agtttgatcg tcttctccgc
     1561 gccgttggcg atttcttcca gggtcaactg caaacgcacc tgcagatcgc gcccgcgcgt
     1621 agccccgcca cgttctcccc gactgcggcg tggcgtgccg gcgccaaaga actcctcgaa
     1681 gccgccgaag tcgcgcataa aggcgcgcag ggcgtcggag agatcgaagc cgccgaagcc
     1741 gaagtcacta aatccgcccg cgcctgctcc ggcacccgcg ccgacaccca cgtggccgaa
     1801 ctggtcatag cggcggcgct tctccgggtc ttttaagact tcgtaggcct cggtcgcttc
     1861 tttgaacttt tcctcggcct ctttggagcc ggggttgcgg tcggggtgat gctggagcgc
     1921 taacttccga taggcctttt tgatctcatc ttcggaagcg ccccgctcca cgctcaacac
     1981 ttcgtagtaa tcgcgcttgg ccatagtttc catgctaatt tatccctgag gaagaagaga
     2041 gtcaatttcc acgcattcga tcgtccgtaa tacgcaaaag ggcgagcaga agttgcccgg
     2101 gcttcgaccg gctcaaaggc gagcccacac gggtcgtacc gaccgattta ccttaaatga
     2161 aggtaaatcc gaaaaagcca gcgaaaatga aagagtaaag gcaaacgcct tgcgcgggac
     2221 gtttgccttc tcagtgacct ttcttggtgc cccggaggtg ggcacttgct atggcaacac
     2281 cctgcctctt gcaaccgtcg aactttgagg gcgcaacaga acgtgaagac agtgactgaa
     2341 cgtcagccct tacgtctgcc gctatttccg gttccccttt tctgtccctt cttgcttttt
     2401 catagctttc atcttctgcg caagtccagc ggcatacttc tcatactgat cgctgtccac
     2461 ggtgaacgtc gtgaacatgt agttggattt ctcatagttc cagttctgct ccgcatcgga
     2521 gtaagtaagt ttggttgtct gtccctgctg atactcctga tgaatcggat cagcgcccgg
     2581 tgggttgacg attttgggcg tgccgccgag ccctccctcg atcaggtgcc cgtggaaact
     2641 cccgtagtca ccaatcgtga ttttatgctc cttgccttcg atcgcccgaa caatcggaac
     2701 gtagcggatt ttaacgtttg gcgacgaagc gtgagatgaa agcaccgtct tcccgagctc
     2761 ttcgagcgcc tggcgctgtt ccgggctggc cttctcatcg atgtagaggt aggagaactt
     2821 ccacttgcca aaggactcca tcatcgactg gccgggaggg ctttccacca tgttggcaac
     2881 cgccaggcct ttgagcggca cgtccccata ggtgccttct tcaatgaaga gaacctggcc
     2941 accaccacag gtcattcgtg ttggtttgga atcgaaccag cacgggcagg ctggattaca
     3001 ggaacaagcc tcttccagtt gccccgtgat cttccaatac gttttcttcg cttccccgtt
     3061 tttcgtgtca ccggcagtcg caatcgtagc gacgctgatg aagagagtgg ataaggcgag
     3121 gagcacgaag agctttacac cctgtttcat agtacctcct ttcgcttagc ggcctcaatt
     3181 atagaagcaa tgagtcctgt ttgtcaagaa tttatttctg tactcgctaa attattctgc
     3241 cggccaaacc caggcaatct gttctgcgaa gaaaatagat gacatccctt tggataccag
     3301 taggttcaag aggctatata aagttttgct ttaatcgaaa tgccgcggat gcgggccttc
     3361 aagatttttc ttcttcccgg ttctcaggag atgctcgcgt cttgcctttg gacaccacga
     3421 cccgcgccgg gcggaggact ttgtcgttaa gcagataacc ggtggagacg acctcgataa
     3481 ctttaccttc cggctggtcg gattcgacgt acattatcgc ctcgtgaaag tgcgggtcga
     3541 aaacctgacc cgctggttcg atgatctgca ctccttcccg gttgagaatg tcgaacatct
     3601 gggtgtagat cagttcaacg cctttgtgga agctgtcaaa atccgcggag gttttggccg
     3661 cgtccagcgc ccgatgaaag ttgtcgacga tgtcgatctg ttgcagaata agattttcat
     3721 tggcgttttt gatgatcgtg gcgaggtcgc ggttggtgcg cttgcggaag ttatcaaact
     3781 cggccgccag ccgcaggtag cggtcttcca actccttgaa gcgcgtctcg ctttcgtcta
     3841 actgattgcg gagcgtttct tccagcgtgg gaaccgcctc ggtcgtagac tcgactcttc
     3901 gaggcggcgc tcccgcttcc tgagcagacg gtagcgggac tgtgtttcga accgtcactt
     3961 tctcttctcc tttttctttt ttcttactca tcacttaccc tcgacacaca aagaagggcg
     4021 gcgcacttca ccgcccctgc agacgctcgg cgattcccgg ttctacgaga aaatttcgct
     4081 caatactttg gcggtgtaat cgaccaccga gaccagtttg gaataaggca tccgggtggg
     4141 gccgatgacg ccgatcgtgc cttttacctt ccccgcctga taggtggagg tgacgacgct
     4201 caaattctgg ccttcggccc ggggattctc gctgcctatg gtaatgacga tgccttcttt
     4261 gattccctgg gtgttcagca tctcgacgat cgacttccgc tcttccaaaa gcgagaagag
     4321 cgtggtcaac cggttgtgat ctttgaactc cggctgattc accacgttgg ccgtaccttc
     4381 aatgtggagg tgctcggcct cctcggcgga aaggagctgg tcggaagcgt tcaggaacag
     4441 gcgcagcagt ttcgggtcac cttccccggg agccgaaagg cgctcccgaa tggtgcgctt
     4501 gatttcgccc agagtgaggc cggccaggcg ctcgttcaaa agccgcgccg tttcccctat
     4561 cagccgttcg tctatggcca cgtcaacttc caaaaggacg ctcttggcca ggccggattt
     4621 aaccgcaatg atgaccaaaa gtttccgctc ggtcaccggc accagatcga tgcgggtcaa
     4681 gaccccctgc tcaaaactgg gggcgatggt cactcccagc tggtgcgaca cgccggccag
     4741 aacccgcgag gtctgctcca gaatccggtc caccgccacc tggtcgagca ccacctcgtt
     4801 gcggatccgc tctttttcct cttccgtcaa ctgttccggg cggagcaggt tatccacgta
     4861 gaggcggtag ccggcgtcgg tgggcacacg ccctgcagac atatggggct gttcgaccaa
     4921 cccgcgcgac tccaaatcct gcatcgtgtt gcggatggtg gccggagaca agccgaaggg
     4981 atagcgggtg gccagaatcc gggagccgac cgggtcggcg gtgcggatgt agtggtcgat
     5041 taaggcgtgg agaatctccc gctcgcgttc attcaagttt tcgaagggct tagtcatagg
     5101 agtaattata atttatagaa aaatttgctc ctgtcaagaa ttaggacgga ctaacgccgt
     5161 gggcttcagc gtacgaagga gactaacagg atcgagagca caatcagcgt accggcggtt
     5221 acgatggcaa tggcattgaa gaccgggccg ttgacgtact tgcccatgag ccggcggtcg
     5281 ttcaccagga gcagcatgaa gactaaaatc agcggcagga cgatgccgtt gagcacctgg
     5341 gagaaataca tgattttaat gagcggtatc cccggcagaa ggacggtgac ggcgccgcct
     5401 aaaatcagaa gcgtgtagag gaggtaaaag aggggcgcct cggagaactt cttgtccacg
     5461 ccgtcctgcc agcccatccc ctcgcagacc atgtaggcgg tggagagggg cagaatcgag
     5521 gccgagaaaa gcgaagcgtt gagcaggccg aaggcaaaga ggtaggaagc gtattttccc
     5581 gccagaggcg ccagggccag cgccgcttcc ttggccgttt cgatctgaac tcctttgacg
     5641 aacagggtcg ccccgcaggc cacgatgatg aagaaggcca ccacgttgac gataaccgag
     5701 ccggaaatca cgtcccagcg ggagtgcttg aggtggttga catgaatttt cttctccacg
     5761 atggccgact ggaggtagaa ctgcatccag ggggcgatgg tggtgccgac cagaccgatg
     5821 accatggcca gggactttga gtcgaaagaa atgtcggtgg ctttcacagc ccggagcacc
     5881 tccgaccagt ccggcctcgc cataagcgca gagacgatgt aggtgaagta gaccaggcag
     5941 gcaaccagaa agaccttctc cacggtcttg taatttccct tcaccaccag aagccagacg
     6001 agaatagctc ccaccggcac cgagagatgc cggctgatgt tgaagatttc cagactggcg
     6061 gcgataccgg cgaattcgga gaccgtattg gcgtagttgg tgatgacgat ggccaagagc
     6121 aggtagaccg ttgtgcgcac gccgaatctt tcgcggatca agtcggcgag ccctttgccg
     6181 gtgaccaccc ccatgcgggc caccatttct tgaatgacga tcagggccac gacgactgga
     6241 atcatcaccc aaaggaggtt gtagccgaac tgcgcgccgg ccagagagta ggtggtgatg
     6301 ccgcccgcgt cgttgtcgac gttggaggta atcatgcccg gccccagaac cgctaagaaa
     6361 atcagaaagg tctggaagcg gcggcgcagg cggtggcgga ggcgcatggg tcaggcaatg
     6421 gtgcggtttc ggaaaagggc ctcttcctca ctcggagacg gttcgctttc cacttgatac
     6481 tcgtgaaaca gcccgccgtg tgagttggtc ggcttcgggt taacacgact ctttgaccgt
     6541 ttgcggttca ggtacggcaa aagcgtatcg accagactga tttgcccccg tacgaccggc
     6601 attccacagc gagcggcgac atcttccagc gtgaggtcat cgaggaatat accgtcggtg
     6661 ttcaagcagc tgggaggcag gaggacgtaa ttgtacttct ggccatgctc tttaattgcg
     6721 tcaacaatat ctcccgcagt caaaaggccg ctcacggtga ccgaggggcc gtagaatttg
     6781 ttttcgacga ttaaaagatc gcactgcaaa ccggagaagt acctcagttc aggtattatt
     6841 tcgctacgaa agaccttccc ggcggaggtg ccggtaacaa tcaaaatctt gacaggtttt
     6901 gtaagacgct tggggagttt gcttttcctt gcctgaaagt catctaaaaa tcgccgcacc
     6961 atgccgatac cgttttccac ctgtgggaag tcgtcatagt agtctgccgg gggtacgagc
     7021 caatcggcca gaaggaagaa ttcgtcggcg gggtaaacga agtgggtgcc taatttttcc
     7081 ttcaaccatt tttgccggga atcgaggtag gtgatcacct cgcgggcgta gtcgcggtcg
     7141 acggagtgca taggcgtgag gcgggcgcgg tactgggtta aaccgacggg gacgacggcg
     7201 agggatggaa tcttaggata aaaatgcgcc aggtcgcgga tggtcttttc cagaaacgcg
     7261 ccgtcattca agccggggca gaggacgacc tgggtgtgga ggatgatgcc ggcctccatc
     7321 agccgtttca
//
LOCUS       DBNGZA010000144         1466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1192773, whole genome shotgun sequence.
ACCESSION   DBNGZA010000144 DBNGZA010000000
VERSION     DBNGZA010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1192773"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="ftsA"
                     /locus_tag="AC16FU_03150"
     CDS             complement(<1..753)
                     /gene="ftsA"
                     /locus_tag="AC16FU_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR2993405.1"
                     /translation="MVGAETVAGLDLGTTKVSVIVAEVERDLWPKLLGIGVAPAAGIR
                     QGQVVDLPAAIHSIRQAIRDAELMAGVKITRVVVGISGEQVQNFHSGSMVSVGTPRLR
                     QEVSPADVGRVLAAARLLEFPSDRQVLHVLPTRFAIDDQKGVRQPVGMIGARLSADVQ
                     VVTCGRTATENLLRAVEKTELEVSELVAEPLAASRAVLTPEEKETGVALIDLGGSTTQ
                     LAVFLEDALALTACIPLGGRNATNDLAVGLRLP"
     gene            complement(740..>1466)
                     /locus_tag="AC16FU_03155"
     CDS             complement(740..>1466)
                     /locus_tag="AC16FU_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR2993406.1"
                     /translation="TVLGFLLAVGTATAERAGLFRLRRVRVSGNTVVSLEEILQRAQL
                     PFGLNLFRVDIEKITHRVAADHRFWGVTVSRQLPDAVEISVTEKKPALTLNTGQLWGL
                     TANLEVLPEGEVSPSLPVVTAGYWQPDRFYQSNRQNGLGLAAEVYRALVAYDSRLLET
                     ISEITVAQSGDVVLYLLPKGVAINLGTGDFSIKVRRLAALLAEFGGILPDLVYDLRFS
                     GNLFVTPKDSLSVAKARNRRGRG"
BASE COUNT          291 a          479 c          431 g          265 t
ORIGIN      
        1 gggcaagcgc aagcccaccg ccaggtcgtt ggtggcattc cgcccgccca ggggaataca
       61 ggcggtcaag gccagggcat cctccaggaa aacggctaac tgcgtggtgc tgccgcccag
      121 gtcgatcaaa gcgaccccgg tttccttttc ctccggcgtc aaaacggccc ggctggcggc
      181 caaaggctcg gccaccagtt cgctcacctc aagttcggtt ttctctacgg cccgcagcaa
      241 attttcggtt gccgtccggc cgcaggtgac gacctgcacg tcggcggaaa ggcgcgcgcc
      301 aatcatgccg accggctgcc gaaccccctt ttggtcatcg atggcaaagc gggtcggcag
      361 gacgtgcagc acctgccggt cggagggaaa ttccagaagg cgggccgccg ccagcacccg
      421 cccgacgtcg gcgggggaaa cctcctggcg taaacgcggg gttcccaccg acaccatgct
      481 cccgctgtga aaattttgta cttgctcgcc ggaaattccg actaccaccc gggtgatctt
      541 aactcccgcc atcagttcgg cgtcccgaat cgcctggcga atcgagtgga tggctgccgg
      601 gaggtcgacc acctgtcctt gccggattcc cgcggcggga gccacgccga tacccaaaag
      661 tttcggccag aggtcgcgct ccacttcggc cacgatcacg ctcaccttgg tggtgcccag
      721 atcgagcccg gcgaccgttt cagccccgac cacggcggtt cctcgctttc gcaacggaaa
      781 gtgaatcttt cggcgtgacg aaaagattgc cggaaaacct gaggtcgtag actaagtcgg
      841 gcagaatgcc gccgaactca gccaaaagcg ccgctaatcg ccggactttt atcgaaaagt
      901 cgccggtgcc caaattgatc gccacacctt tcgggaggag gtaaagcacc acgtctccgg
      961 actgcgctac ggtaatttct gagattgttt caaggagtcg ggaatcgtag gcaaccaggg
     1021 cccggtacac ctctgccgcc agacccaacc cgttctgccg gttcgattgg tagaaacggt
     1081 cgggctgcca gtacccggcg gtcaccaccg gcaaggatgg ggagacttcc ccttccggga
     1141 gcacttccaa attggcagtg agaccccaga gttgcccggt gttcagggtc agggccggtt
     1201 ttttttcggt cacggaaatc tcgaccgcgt cgggcaattg tcgcgaaacc gttacccccc
     1261 aaaacctgtg atccgcggcc acgcggtgcg tgattttttc gatgtcgacg cgaaacagat
     1321 taaggccgaa cggcagttgc gcccgctgga gaatttcttc caggctgacc accgtattac
     1381 cgctcaccct gactctgcgg agccgaaaca gtccggcccg ctcggcagtc gccgtcccga
     1441 ccgccaagag aaaccccagc acggtg
//
LOCUS       DBNGZA010000145         4194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2570724, whole genome shotgun sequence.
ACCESSION   DBNGZA010000145 DBNGZA010000000
VERSION     DBNGZA010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2570724"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="AC16FU_03160"
     CDS             complement(<1..971)
                     /locus_tag="AC16FU_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR2993407.1"
                     /translation="MIELSAGLVVVLVLVLAAEFVNGWTDAPNAIATVVSTRALSPGK
                     AVVLAAVLNAVGALSGTAVAATIGKGIVRPEVINLTTVGAAMVAIVFWSTLAWKYGLP
                     TSESHALVAALAGAGLATAGPSVLLWVGWKKVLIGLVFSTFLGFGVGWAIVKVILRFF
                     AQMTPTRARKVFGRMQLVSAAFMAFSHGSNDGQKFIGTFSLALVLGGVLPEFRVPWEV
                     ILICSLIMGISTSVGGWRIIKTMGMQMVKLETYQGFSAEAGAASSIELASRLGIPVST
                     THTIGTALMGVGASRRLSAVRWGVAGQVVTAWILTFPVCGFIGWLVAT"
     gene            complement(1091..1711)
                     /locus_tag="AC16FU_03165"
     CDS             complement(1091..1711)
                     /locus_tag="AC16FU_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR2993408.1"
                     /translation="MGLLPAKEKFFDMLDRAAKNVTQGVEEFQAMLADYAHREEHVKK
                     LDAMEHEGDIITHEIMDKLNRSFVTPIDREDIHALASGLDDVIDVVEEAAHRMYLYRI
                     EKPNPRLLEQAEILLKAVQEVCRAVTSLRDLKRPRRILDYAIEINRLENEGDVALNRN
                     VAALFEGQVAPLEVIKWKEIYEHVEAALDKCEDIAVIIESVVVKNA"
     gene            complement(1711..2184)
                     /locus_tag="AC16FU_03170"
     CDS             complement(1711..2184)
                     /locus_tag="AC16FU_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993409.1"
                     /translation="MHLVALVGAAMLVLGVFLPWMQVGIFQERGIDALDGGLVLAAGL
                     LGLIVAGHNLARGRNEYRWVYLPDGVLALIISLVALARIGQRAAKITGALEEILRIFG
                     GTEKIKTSELVGSGLVVVIIGSMLLLAAGLGQVVGSKFAPPRGDTEGTNSAKEST"
     gene            complement(2291..3499)
                     /locus_tag="AC16FU_03175"
     CDS             complement(2291..3499)
                     /locus_tag="AC16FU_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993410.1"
                     /translation="MRFQETAESILAGAAKGKALSALWTFPSILFAAFCIAWAAEAAQ
                     VLVSRGLALAVLAWLQTAPEFAVEGVIAWRAGKVPTPENIGLMTANFTGALRLLVGFG
                     WPGVYFMCWLGHRRKGNSGLPVIRMEGEDSIPIVGMIVPLLYAFFIAIKGSLSLWDAA
                     LLIFIYSIYLWILYRVPPRQEEDLEEMERLPKWILSHTKPRRNALILLFFGAGAILII
                     TMAHPFVESMLGVAVWVGISSYFAVQWIAPFLSESPEFLSAFYWARRSAKAPMALLNT
                     VSSSIAELTLLIALIPIVYSLSGGHFSAVPFDGPHRMEILLTAVQALLGFLLLANFRF
                     HWYEAAGIFALWFVQFIIPHLREEVIFAYGFWILVEFTLIMVGQQQLPAFAAFGRCLR
                     QHGILVKKSA"
     gene            complement(3499..>4194)
                     /locus_tag="AC16FU_03180"
     CDS             complement(3499..>4194)
                     /locus_tag="AC16FU_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR2993411.1"
                     /translation="DQVTGLIFAVAVTRRDLPEKTIDAKPLRVICLFLTPRHMARLYL
                     QTMSALVTFARTEGLVEHLLQAKTAEELILFFRKANIEVRRDLVARDIMRRQVVKATL
                     QMSLKEVANLLFKHRISGVPVVNAEEEVVGEVTEKELIKAAIPNYSRLVDNARAHLEV
                     DPFETLIQKEDKITARDLMASKFVTRSEETPVVELAALMLLNNVRRVEIVRDHKLVGI
                     VVRSDIVGKIIRG"
BASE COUNT          916 a         1359 c         1150 g          769 t
ORIGIN      
        1 gtcgccacca gccacccgat gaagccgcag accgggaagg tcagaatcca ggcggtgacc
       61 acctgccccg ccacgcccca gcgcaccgcc gaaagccgcc gggaagcgcc gacgcccatt
      121 aaggccgtgc cgatggtatg cgtggtgctc accggtatgc ccaggcgcga cgccagttcg
      181 atactggagg cagctcccgc ctcggcggaa aagccctggt aggtttctaa tttcaccatc
      241 tgcatcccca tcgtcttgat gatccgccag ccgcccaccg acgtgctgat ccccataatg
      301 agagagcaaa tcaaaataac ctcccagggt acccggaatt cggggagaac tcctcccagc
      361 actaaagcca gcgaaaacgt cccgatgaac ttctggccgt cattgctgcc gtggctgaaa
      421 gccataaagg cggccgagac caactgcatt ctcccgaaca ccttgcgcgc cctggtcggc
      481 gtcatctgcg caaagaagcg taaaatgact ttgacgattg cccagccgac cccgaaacct
      541 aaaaacgtgg aaaagacgag accgatgagc acctttttcc atcccaccca gagcaggacc
      601 gacggaccgg cggtggccaa ccccgctccg gccagagccg ccaccagggc gtggctttcg
      661 gaggtcggca ggccgtactt ccaggcgagc gtgctccaga agacaatcgc caccatcgcc
      721 gctccgacgg tggtaaggtt aatcacctcc gggcggacga tccctttgcc gatggtggcc
      781 gccaccgccg ttccggagag cgctccgacg gcattgagca ccgccgccag aaccaccgct
      841 ttccccggcg aaagtgcccg tgtggagacc accgtggcga tggcgttggg cgcgtcggtc
      901 cagccgttga caaattccgc ggccagaacg aggacgagga cgacgaccag tcccgccgaa
      961 agctcaatca tgaccactct cccccgcggg caacgacttt atgtatgcac aaaaaaatgt
     1021 ttgcccctgt agccgaggcc atacgggccg tatgggattg tatcctcggt ccaattcggt
     1081 tctgcccgcc tcacgcattc ttcaccacca cgctctcgat gatgaccgcg atgtcctcgc
     1141 acttgtcgag ggccgcctcc acgtgctcgt aaatctcctt ccatttgata acctccaaag
     1201 gcgccacctg accttcaaag agcgccgcca cgttgcggtt caaggccacg tccccttcgt
     1261 tttccagccg gttgatttca atggcgtagt ccagaatccg ccgcggtcgt ttcagatcac
     1321 ggaggctggt caccgcccgg cagacctcct gcaccgcctt gagcaggatt tcggcttgtt
     1381 ccagaaggcg cgggttcggc ttttcgatcc ggtacaggta catccggtgc gccgcctcct
     1441 ccaccacgtc gatcacgtcg tccaggccgg aggcgagcgc gtggatgtct tcgcggtcga
     1501 tgggcgtgac gaaggagcgg ttcagtttat ccatgatctc gtgggtgatg atgtccccct
     1561 cgtgctccat ggcgtccagc ttcttgacgt gctcctcgcg atgcgcgtag tcggccagca
     1621 tcgcctggaa ctcttccacc ccctgggtga cgttcttggc ggcgcggtcc aacatgtcga
     1681 aaaatttctc tttcgcgggc aaaagtccca ttacgtgctc tccttcgctg aattcgttcc
     1741 ctcagtgtct ccccgcggcg gtgcgaactt gctcccgacc acctgcccga gccccgccgc
     1801 caacaagagc atactgccga tgatgacgac caccaaaccc gaaccgacca gttcgctcgt
     1861 tttgattttt tccgtcccac cgaaaatccg gaggatctcc tccagcgctc cggtgatttt
     1921 ggccgcccgc tggccgatcc gtgccagggc gacgagactg ataattaagg ccagaactcc
     1981 gtccggcagg tagacccagc ggtactcgtt ccgcccccgg gccagattgt gcccggcgac
     2041 aattaacccc aagaggccgg cggccaaaac cagtccgccg tccagggcat caatcccccg
     2101 ctcctggaaa atacccacct gcatccaggg caaaaagacg cccaggacga gcatcgccgc
     2161 acctaccagc gctacgaggt gcatgaaact tttccccgcg tgggcgggcc gccctcggcc
     2221 cgccaagact caagtccgtt acgaaagaca tggttagtgg ccttcagcgc ctttatgaat
     2281 attaatcgca ttacgcgctc tttttcacca gtatcccgtg ctgcctcaaa cagcgtccaa
     2341 aggccgcaaa cgccggcaac tgttgctggc ccaccataat cagagtgaac tccaccagaa
     2401 tccagaagcc gtaggcaaaa atcacttcct cccgcaggtg gggaatgatg aattgcacga
     2461 accagagggc gaaaatcccc gccgcctcgt accagtggaa gcggaaattc gccagcaaga
     2521 gaaatcccag aagcgcctgc accgcggtca aaagaatctc catccggtgc ggcccgtcaa
     2581 acggcaccgc cgaaaaatgc ccgcccgaaa gggaatagac aatcggaatg agcgcaatca
     2641 aaagcgtcaa ttcggcgatg gaagacgaga cggtgttgag cagagccatc ggcgccttgg
     2701 cgctgcggcg ggcccagtag aaagcggaga ggaattccgg cgactccgag agaaagggcg
     2761 caatccactg caccgcaaag taggacgaaa tccctaccca gaccgctacg cccaacatgc
     2821 tctccacgaa cgggtgggcc atggtaatga tcagaatggc gccggcgccg aagaaaagga
     2881 gaatgagtgc gttccgcctg ggcttggtgt gagagagaat ccacttgggg agccgctcca
     2941 tttcctccaa atcttcttcc tgccggggag gaacgcggta cagaatccag aggtaaatcg
     3001 agtagatgaa aatgagaaga gccgcatccc aaagcgaaag cgagcctttt attgcaataa
     3061 aaaaggcgta gaggagcggc acgatcatcc cgacgatggg aatcgaatcc tctccttcca
     3121 tccgaatgac cggcaaaccc gaattcccct tccgccggtg tcccagccag cacatgaaat
     3181 agactccggg ccagccgaag ccgaccaaaa gccgcaaggc gccggtgaag ttggcggtca
     3241 tgagcccgat attctccggc gtcggcactt ttcccgcccg ccaggcaatc accccttcca
     3301 ctgcaaactc tggcgcggtc tgcagccagg ccagaaccgc cagggccagt ccgcgggaaa
     3361 ccagaacctg cgccgcttcc gccgcccagg caatgcagaa cgccgcaaag agaatggaag
     3421 ggaaagtcca gagagccgag agggccttgc ctttggccgc tccggccaga atcgattccg
     3481 ccgtctcttg gaagcgcatc agccccggat gattttgccg acgatgtcgg aacggaccac
     3541 aattccgacc aatttgtggt cccggacgat ttccacgcgc cggacgttgt tcaaaagcat
     3601 gagcgctgct aactccacta ccggcgtctc ttcgctgcgg gttacgaact tggacgccat
     3661 caggtctctg gcggtaatct tgtcttcttt ctgaatcaag gtctcgaacg gatcaacctc
     3721 caggtgcgcc cgggcattgt ccaccaagcg ggagtagttc ggaatggcgg ccttgattaa
     3781 ctctttctcg gtgacctctc ccaccacttc ttcctccgca ttgaccaccg gcacgccgga
     3841 gatgcggtgt ttgaacagga ggttggccac ttctttcagg ctcatctgga gcgtcgcctt
     3901 gaccacctgc cgccgcatga tgtcgcgtgc caccaggtcg cgccgcacct cgatattggc
     3961 tttccggaaa aaaagaatga gctcttccgc ggttttggcc tgcagcaggt gctccaccaa
     4021 cccttcggtg cgggcgaagg ttaccagggc cgacatcgtt tgcaaataga gccgcgccat
     4081 gtgccgaggg gtgaggaaaa ggcagattac ccgcaggggt ttggcatcga tggtcttctc
     4141 cggtaaatct cttcgggtga cggcgacggc gaagattaat cccgtcacct gatc
//
LOCUS       DBNGZA010000146         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1938461, whole genome shotgun sequence.
ACCESSION   DBNGZA010000146 DBNGZA010000000
VERSION     DBNGZA010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1938461"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16FU_03185"
     CDS             complement(<1..224)
                     /locus_tag="AC16FU_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008639903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR2993412.1"
                     /translation="MNGEPVILSACRTAIGNFLGGLATVSAPRLGAVAVREVVKRAGI
                     EPNEVDEVMLGHVVQAGAGQAPARQAAIHGG"
     gene            complement(255..1163)
                     /locus_tag="AC16FU_03190"
     CDS             complement(255..1163)
                     /locus_tag="AC16FU_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993413.1"
                     /translation="MRFLLVLFITVGGHSRFAVVAFAQRDLPLCAVKQADPPQAGWYV
                     EPMVFVHRESESPVVYRYPQPGEAIWKYAFPGGARFDLFRWGVKVGEFVLKRFQSSLE
                     DSCQGARAWGQPAWGTTIESPDELIAVRTGEYPEAQPNWSALDSVAASGEAIPQTRQL
                     LQKQGVPAGVTLKCEVSQLQTFQINHTPFFAVTLAYRPPAGDSTSAEQISRGVGALVI
                     TKLLRRSKKESLYQPVLTRVAQWDENEPPVTWDLVGLFDADQDGMPELATRRKENGTY
                     TYEISVMESGRYRSVYESDRRGCAQK"
     gene            complement(1260..1967)
                     /locus_tag="AC16FU_03195"
     CDS             complement(1260..1967)
                     /locus_tag="AC16FU_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HIR2993414.1"
                     /translation="MKNPTVSPAAKEPQKSQGADPRELIIRRAAKELRDGYYVNLGIG
                     MPTLVANYIPPGMDIVLQSENGLLGIGPFPYAGEEDADLINAGKETVTALPAASYFSS
                     ADSFAMIRGGHIDLSILGAMEVDQEGNLANWVIPGKMVKGMGGAMDLVAGAKRVVVTM
                     LHTAKDGSSKVLKQCKLPLTGRKVVHRIISDLAVMDVTPQGLLLLEVAPGVTVEQVKK
                     STEADLAVSNRVREMEG"
BASE COUNT          381 a          634 c          527 g          440 t
ORIGIN      
        1 ccgccgtgga tcgccgcctg ccgggccggg gcctgcccgg cgcccgcctg cactacgtgg
       61 cccagcatga cctcatcgac ttcattcggc tctatacccg cgcgcttgac cacttcccgc
      121 accgcgactg cacccagccg cggcgccgac accgttgcca gaccgcccaa aaaatttccg
      181 attgccgtcc ggcaggccga cagaatgact ggctctccgt tcatatcccc tcctcactcc
      241 gaaaaccggc gttcttattt ctgtgcacaa ccccgcctat cactttcata gacgctgcga
      301 taccgtcccg attccataac ggaaatctca taagtatacg taccattctc tttccgccgt
      361 gtggctaact ccggcatacc gtcttgatcg gcatcaaaga ggccgaccaa gtcccaggtg
      421 accgggggtt cgttttcgtc ccattgcgcg acccgggtga gcacgggctg atacaggctt
      481 tcttttttcg aacggcgcag aagtttcgtt atgaccaggg cgcccacgcc ccgagagatc
      541 tgctcggctg aagtcgagtc cccggccggg ggccgatagg ccaaggtgac ggcaaagaac
      601 ggcgtatggt ttatttggaa ggtttggagc tggctcactt cgcattttaa cgtcactccc
      661 gcgggaacgc cctgcttctg gagcagctgc cgcgtttggg gtatcgcctc gccgctcgcg
      721 gcaactgaat caagcgcact ccagttcggc tgagcttccg gatattctcc ggtgcgcacc
      781 gcgatcagtt cgtccggact ttcgatagtc gtcccccacg ccggctggcc ccaagcacgg
      841 gcgccctggc aggaatcttc cagagaggac tgaaatcgct tcagcacgaa ttctcccact
      901 tttacgcccc agcgaaagag gtcgaaccgc gctcctccgg ggaaagcata cttccagata
      961 gcctcacccg gctgggggta tcgataaacc accgggctct ccgactctcg gtgcacaaaa
     1021 accattggtt ccacgtacca gcccgcctga ggcgggtccg cctgtttgac tgcgcagagg
     1081 ggcaggtcac gctgggcaaa cgccacgacg gcaaaacgcg aatgtccacc gaccgttatg
     1141 aaaagtacga gaagaaatct cacgcgcaat ccgttgcagc gctgttttta ctgttattcg
     1201 tatggctttc cttataacac ccgtaaaggt aaaggacgag aaccattgct attttctgtc
     1261 tatccttcca tttctcttac tcggttactg acggccaaat ccgcctcagt ggactttttg
     1321 acctgttcca ccgtcactcc cggtgccact tccagcagga gcaatccctg cggggtcacg
     1381 tccatcaccg ccaggtcgct gatgatgcgg tgaaccactt tccttcccgt cagcggcagt
     1441 ttgcactgct tgagcacctt ggaggagccg tccttggcgg tgtggagcat ggtgacgacc
     1501 acccgcttgg cgccagcgac cagatccatc gcgccgccca ttcccttgac catttttccg
     1561 gggatcaccc agttggccaa gtttccttcc tgatccactt ccattgcacc taaaatcgag
     1621 aggtcaatat gcccgccgcg gatcatcgcg aaagagtcgg cgctggagaa ataggaagcg
     1681 gccggcagcg cggtgaccgt ctccttcccg gcgttaatta aatccgcgtc ttcttcccct
     1741 gcgtagggga aaggaccgat ccccaagagc ccgttttccg actggagcac gatatccatt
     1801 cccggtggga tgtagttggc gaccaaggtc ggcatgccga tgcctaaatt gacgtagtag
     1861 ccgtcgcgaa gctcttttgc ggcgcggcgg atgatcaatt cccgcgggtc agccccttga
     1921 gatttctgtg gttctttcgc tgctggagat acggttggat ttttcatcgg gttgtgaccg
     1981 cc
//
LOCUS       DBNGZA010000147         7799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_664068, whole genome shotgun sequence.
ACCESSION   DBNGZA010000147 DBNGZA010000000
VERSION     DBNGZA010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_664068"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(593..1417)
                     /gene="mutM"
                     /locus_tag="AC16FU_03200"
     CDS             complement(593..1417)
                     /gene="mutM"
                     /locus_tag="AC16FU_03200"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR2993415.1"
                     /translation="MPELPEVETIARSLRKRMIGRTIITARLFCPKLQRENPPDFSSR
                     VAGSTVQSIQRKGKHLIFLLSDGWAFWVHLMMTGHLFLLQKSHPLVKADRAVFKLSGG
                     LELRFHDIRRFGHLRLVRQQAIGNLKEYRRLGPDALFVSEEEFCSRLRSRKRQMKPAL
                     LDQTLLAGLGNIYADEALFAARIHPLTRTDRLSRPQLRKLWSEIRRLLTRAIHLRGTS
                     VYSYTDTDGRRGKYQEKLKVYGQENAPCPRCRAEVKRLVIGQRSSHFCPRCQKRGA"
     gene            complement(1462..1941)
                     /locus_tag="AC16FU_03205"
     CDS             complement(1462..1941)
                     /locus_tag="AC16FU_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR2993416.1"
                     /translation="MSTSGKTVAPRATESETSAGSPSAEFPVGRRNRFYSAYLPAILY
                     ALLIFGLSSIPNLQAPNLGIGPVDKLVHAVEYAIFGVLLSRAVTGFPRLRNHILWRPL
                     VIAVGGLYAASDELHQLLVPGRSAELADWVADVAGLVIGLFILLPLVHRLLKKKVKT"
     gene            2412..3194
                     /locus_tag="AC16FU_03210"
     CDS             2412..3194
                     /locus_tag="AC16FU_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2993417.1"
                     /translation="MKTTPTHDSKQPLKGKVALVTGGSRGIGRACALKLAEKGARVAI
                     GFFKSREKAKKTQAEIAALGGDCQTVRVNLAKTDSVEKMFAEMKAHYGRLDILVCNAA
                     MGIFGLTLDISLEDWELTMDANTRSFFLCAREAVKLMLPHGGGKIVGLTSYGSQRFIP
                     AYAAMGVAKAGIETLTRYLAVELVPKGINVNCVSGGPVDTDSLRLIPGADKVVEESVK
                     RTPAGRIGQPEDIANIVAFLCTDEANWICGQTIVADGGVSLI"
     gene            3467..3676
                     /locus_tag="AC16FU_03215"
     CDS             3467..3676
                     /locus_tag="AC16FU_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993418.1"
                     /translation="MKKMVMAVLTLVLGLSFVAGILISRAEAACYTQCQYCPNCHLLQ
                     CCNGVCVDTGKSCGSFCRFVDCNPP"
     gene            complement(3744..4226)
                     /gene="bcp"
                     /locus_tag="AC16FU_03220"
     CDS             complement(3744..4226)
                     /gene="bcp"
                     /locus_tag="AC16FU_03220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR2993419.1"
                     /translation="MPTETALAEGAPAPQFTLSADTGQKISLADFAGKTNVVLYFYPK
                     DDTPGCTKEACSFRDLKKEFEKAGAAILGVSMDSVDSHKKFKAKFKLTFPLLADEDGK
                     VTRAYGVYKEKNMYGKKYWGIERTTFVINKQGKIAQIFPKVQVDGHDREVLGVVKVLK
                     "
     gene            complement(4432..4818)
                     /locus_tag="AC16FU_03225"
     CDS             complement(4432..4818)
                     /locus_tag="AC16FU_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993420.1"
                     /translation="MKRILTPVLAFVAVCAFTVMVFAQTPAADSQKAAPATKAQTKAP
                     AMKATTKPAAAKSLTFSGRVVSVDTVAMTVVVKGKKAEETFNVEPGTKVKVAGKTAKL
                     ADLKKDAWVKVMYKMKDGRRTATAIM"
     gene            complement(5134..5574)
                     /locus_tag="AC16FU_03230"
     CDS             complement(5134..5574)
                     /locus_tag="AC16FU_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993421.1"
                     /translation="MKKGLVFAGLLVLAFVFLFSSAFAQMSQPKKEEPKKEAMKMEMG
                     PKEMTVTGEVVDLACYIKMGARGAGHKECATECANAGIPLGIVDAKGEIFLPASKEDK
                     KGANAELLPFVAEMVTVKGKLYHKGGQRLLAIESVEKAAAAPAK"
     gene            5725..7614
                     /locus_tag="AC16FU_03235"
     CDS             5725..7614
                     /locus_tag="AC16FU_03235"
                     /inference="COORDINATES: protein motif:HMM:NF025034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HIR2993422.1"
                     /translation="MGRKNYSLQLPVRATHRLILWAVWFFSIWFISPGTGNANEIESI
                     LAKHAQALGGLEKVRALTTSHAQATVTAGGFTGKSEAYEAPPGRFRQDIDLGIFNLSV
                     GCDGTNWWIKDQNGIVRDLGEKEKEEFVTESFFSAYKYLTEDTATFTLRFLPEKSDSQ
                     FYAIAVKPQGGLGRTLFINRRTYLVDKSQQIQDGALITTTYADFRSVDGLLQPFHLHQ
                     SSGQVIFDTDIRITSVETNVAIDPAVFARPGAAARRDFAFAPGKTSTTVPFELVNNHI
                     YFSATLQGSVTGSFLLDTGAGAVVLDSTFAAKSGLVGAGGLEAKGVSGSEKAGLVKIG
                     SLTVGDVTFDSLVAATLDLQPLTQFEGHGIDGILGYDILARVVVEVDYEKSQLTFSDP
                     DLFTYTGTGQVLPMTLDQRIPHVEATVEGQYRGWFNVDTGSRNTLDLFSPFVFEHTLA
                     DKYPKRVETRLGGIGDDLPSTTQMVRMKSFTVGSFEIPDLLVGLSTAQKGIFASEKFQ
                     GNIGSGLLKRFTVIFNYPKQQLILFPNRHFGERDEFDKSGLWLREVAGKFIVEEVVKG
                     TAGDKSGFKRGDAITEINGKPAREYSLNDLRALFRGPDGTKFSLKFQRTNKEKKKELK
                     LKTIL"
BASE COUNT         1681 a         2233 c         2129 g         1756 t
ORIGIN      
        1 aaaaaagaat accccacatc cgcttaaccc tcccgaacat ttgcctcatt ttctcttgcc
       61 acgtaatcat tgccgaaccc gttagacccc gcactacgaa atagcgcgca attaaacaga
      121 tgccctaaaa gttgtcaact cttttttctg gccggtggac gctcgccccg gataatccca
      181 ttcctgcaat aattgccaaa gtttaagcgt aaccgcttgc cgtttagttt cggaggggct
      241 ccgcccgggt gtcgccactc acccgggcag agcatgcgga ggcatgaagc gttcacggag
      301 gtgaggaatt caaacggttc aaacctcact tctctggtgt ggcaaccggc gtgcccgaat
      361 ctcgcggaga attgtggtga ggaaaaatta tccgctattt cttttgtacc aaaaggttac
      421 ggcgtctgat aagacaagaa tgaagggaaa ggtggagtca cttcgtaacc caaaaagggc
      481 ggaaaatcgt cggcgctgac gattctttac ttttgtattt aatcaaccat aaaattgaca
      541 agaacttaat cgatcggcga aaatttgccg ccccgcggac gatttttcaa gattatgcgc
      601 ctcgtttctg acaccttgga cagaaatgac tggagcgctg cccgatgacc agccgtttca
      661 cttcggcccg gcagcgcggg cagggcgcat tttcctgacc gtaaactttc agtttttctt
      721 gatatttgcc ccggcgtccg tcggtatccg tgtaactata gaccgaagtt ccccgcaggt
      781 gaatcgctct ggtcaaaagg cgcctgattt cgctccagag ttttctcagt tgcggccggg
      841 aaagccgatc ggtccgcgtc agcggatgaa tgcgggcggc aaaaagagct tcgtcggcgt
      901 agatattccc cagcccggcc aaaagcgttt gatcgagaag agcgggcttc atctgccgct
      961 tgcggctgcg gagccgggaa cagaattctt cctccgaaac gaagagggca tcgggaccga
     1021 ggcggcggta ctcctttaaa ttcccaattg cctgctgacg caccagccgc agatggccga
     1081 accgccggat gtcgtggaaa cgcaactcta agccgccgga aagtttgaac acggcgcgat
     1141 cggctttcac cagcggatgt gatttttgca aaaggaagag gtggccggtc atcattaaat
     1201 gcacccagaa ggcccagccg tcggataaga gaaaaatcag gtgcttgccc ttgcgctgga
     1261 tcgattggac ggtcgaccct gcgacccggg atgaaaaatc aggcgggttt tcccgctgga
     1321 gtttgggaca gaaaagccgg gcggtgatga tcgtgcgccc gatcattctt tttctgagac
     1381 tgcgcgcgat ggtctcaact tcgggcaact cgggcatgca gattctctgg cagaacggcg
     1441 taattcgcta acacgttttt cttacgtctt gacttttttc tttaacagcc ggtgtacgag
     1501 cggcaggagg atgaacaacc cgatcaccag gccggccacg tccgccaccc agtcggccag
     1561 ttccgcagag cggcccggca cgagcaattg gtgcagctcg tcgctggcgg cgtacagtcc
     1621 gcccacagca atcacgagcg gccgccagag gatgtgattg cggagccgcg ggaatcccgt
     1681 gacagcgcgg gagagcaaaa ccccaaaaat ggcatactcc accgcgtgca caagtttgtc
     1741 caccgggcca atacccaaat tcggcgcctg gagattggga atcgaagaca gaccgaagat
     1801 caacagggcg taaaggattg ccggcaggta ggcggaatag aaccggttcc gccgacccac
     1861 agggaattct gcgctgggcg agccggcact cgtctccgac tcggtcgcgc gcggtgcgac
     1921 tgtcttgccg ctggtgctca cttccccagc atacgcgccg atcgattccc cggcaaataa
     1981 aaaaggccgg agtcgttacc tccagccttc actcaaagaa gaagcgggtt tagcgctttt
     2041 tccccttctt cgcttttttc ttttttttca tcagggcacc ctcacctcct tcggcacagc
     2101 gggttcctcc agattgcccc gtccgaggcg gggcacaaaa aaacccgagt cacgctcggg
     2161 ttcgtctgcc aatgatcgga tactcgacca cgcacttacc accgaaattt ttcaatcgac
     2221 accgcattta cgttaaagta acggacgctg caaaaatttg tcaagaaaaa ttttgcattt
     2281 tgagaaatta ttttcgttcc gccctttcgt gcgcgtaagc gttgaagaga ttttctgcga
     2341 aaggaagtgc tgagcgctgt gtttttctcg tcttgactgc cttccgcacg cggcttatac
     2401 tcgacaccaa aatgaaaaca actcccacac acgactcaaa gcaaccgctg aagggaaaag
     2461 tggcgctcgt gaccggcggc tcgcgcggga tcggccgcgc ctgcgcgctc aagttggccg
     2521 aaaagggcgc cagggtcgcc atcggtttct tcaagagccg ggagaaggcc aaaaaaacgc
     2581 aggccgaaat cgccgcgttg ggcggcgatt gccagacggt gcgggtcaac ctcgccaaga
     2641 ccgacagtgt cgaaaagatg ttcgccgaga tgaaagcgca ctacggccgg ctagatattt
     2701 tggtctgcaa cgcggcgatg ggcatcttcg gcctgacgct cgacatctcc ctggaagact
     2761 gggaactgac gatggacgcc aacacccgga gtttctttct ctgcgcgcgg gaggcggtca
     2821 aactgatgct cccccacggc ggcgggaaga ttgtggggct taccagttac ggctcccagc
     2881 gcttcattcc ggcctacgcc gcaatgggcg tggctaaggc agggatcgaa accctgaccc
     2941 gctatttggc ggtggagttg gtccccaagg ggatcaacgt caactgcgtc tccggcggcc
     3001 cggtggacac cgactccctg cgcttgatcc cgggggccga caaagtcgtg gaagagtcgg
     3061 tcaagcgcac acccgccggg cggatcggcc agccggagga catcgccaac atcgtcgcct
     3121 tcctctgcac cgacgaagcc aactggattt gcggccagac gattgtggcc gacggaggag
     3181 tatctttgat atgaggttgc aaggccaccc tgagcgaaat cgaagagtcg cggacggcgg
     3241 gatttctttt tgacaaattc ctgccggccg tatttttttc gaccgaagta aaaaaaataa
     3301 cagtccctgg ttacggggtg agacggttga gaaaagagac gctttatctt ttgatgattc
     3361 aatgcggctg tagccgcata ttcccgggta tttcggcgag ccgaccagaa agcgggtcgg
     3421 cttttttacg tccaaaacct cattcgaaaa aaggaggtga gtaggaatga aaaagatggt
     3481 catggcggtt cttaccctgg tcctggggct ctcgtttgtc gccggcatcc tgattagccg
     3541 ggcggaagcg gcctgctata cgcagtgcca atattgcccc aattgccacc tcttgcagtg
     3601 ctgcaatggg gtgtgtgttg acacggggaa gtcgtgtggc tcgttttgcc gctttgtcga
     3661 ctgcaacccg ccctaagccc cgaatccacg gaaagaaaac ccctcaaaga atgaggggtt
     3721 ttctttcccg acccggacga cgattacttt aagactttca ccacccccag cacctcccgg
     3781 tcgtgcccgt ccacctgcac cttcgggaag atttgggcga ttttgccctg cttgttgatg
     3841 acaaaggtgg tccgctcaat cccccagtac tttttgccgt acatattctt ctctttgtaa
     3901 accccgtagg ccctggtcac cttcccgtct tcgtcggcta aaagcggaaa agtcagtttg
     3961 aacttggctt tgaacttctt atgggagtcg accgaatcca tcgagactcc taagatcgcc
     4021 gcgccggctt tttcaaactc ttttttcagg tcgcggaatg aacaggcctc cttggtacag
     4081 ccgggggtat cgtctttggg gtagaagtag agaacgacgt tcgttttgcc ggcaaagtcg
     4141 gccaaagaaa tcttttggcc cgtatcagcg gagagcgtga actgcggggc gggagcgcct
     4201 tcggccagag cggtttcggt cggcatcgtt ttactcctca ttttccggca ggtcgttctc
     4261 tgaccagtca aatccggtcg aacggagaca acaaaataga actctcactg cggcggggat
     4321 cggacaccgc cgagtctgat taagaaaata ttctccccct cgagccctaa ccgccacttt
     4381 tagtaaagtg gtgactggct cttggcttta ctgcgcaagc cgggtgttca attacatgat
     4441 cgctgtggcg gtcctccggc catctttcat cttgtacata acctttaccc aggcgtcctt
     4501 tttgaggtcc gccaacttcg ccgtctttcc cgccaccttg actttggtgc cgggctcgac
     4561 gttgaaagtt tcttccgcct ttttgccctt gaccacgacg gtcatagcca ccgtatccac
     4621 cgaaaccacc cggccagaga aagtgagact ctttgccgcg gcaggcttgg tggtcgcctt
     4681 catggcgggc gccttggttt gggctttggt ggcgggagcg gccttctggc tgtcggcagc
     4741 cggggtctgg gcaaacacca tcacggtgaa tgcgcagact gcgacgaacg ccagtacggg
     4801 agttaagatt ctcttcatga aaacctcctt ctttgggtta tgggtgttct aataacgctc
     4861 ttcttctctc gcatcagacg attttatcct catccaatac gtacatttgt tcgctgattc
     4921 taaagtcaag ttttttccgg gacaaaacca ctccggctgg gccgagcccg gtttggcggg
     4981 agcggattct gaattcatag ctcggattcc ggtgttccgg aaatggttct taactttgac
     5041 gcaagaaccc acattgctcc ctccgctgac ccacaaaagc cccgctcggt agtaatcgtt
     5101 ccgaccacca gcggggcttt ggctgtgacg cgcttacttg gccggcgcgg cggcggcctt
     5161 ctccaccgat tcgatggcga gcaggcgctg gccacccttg tggtacaact tccctttcac
     5221 cgtcaccatc tcagccacaa aaggtagcag ttccgcgttg gcgcctttct tatcctcctt
     5281 ggatgcgggc aggaagattt ctcccttggc gtcgacgatg cccaatggaa tcccggcgtt
     5341 ggcacattcg gtggcgcact ccttgtgccc ggctccccgg gcgcccatct tgatgtagca
     5401 ggccaggtcc accacttcgc cggtcaccgt catctccttc ggccccattt ccatcttcat
     5461 ggcttccttc ttgggttctt ccttcttggg ctgggacatc tgcgcgaacg cagacgagaa
     5521 gaggaacacg aacgccaaaa ccagcaggcc ggcgaatacg agtcctttct tcacgcttca
     5581 ctcacctcct ttatgtgata ttgatcatgg ttgcgtattt atgccttata cgaaaaatgc
     5641 ttaccactgt tggaacggac ttttccatca ttttgggggc cagtatcttg accggccctg
     5701 aagggaatgc tatgctttta ccttatggga cgcaaaaact attctctcca gttgccggtg
     5761 cgggcaaccc accgactcat tttgtgggcg gtgtggtttt tctcgatctg gtttatttct
     5821 ccaggaaccg gaaatgcaaa cgaaatcgaa tcgattctgg ccaaacacgc gcaggcgctg
     5881 gggggattgg aaaaggtccg cgccctcacc accagccacg ctcaagccac cgtcaccgcg
     5941 ggcggcttta ccggaaaaag cgaagcgtac gaggcgccgc cgggccgctt ccgccaggat
     6001 atcgatctgg ggatcttcaa tctgtcggtg ggctgcgacg ggacgaactg gtggattaaa
     6061 gaccagaacg gcatcgtgcg cgatctgggc gagaaagaga aagaggagtt cgtcaccgaa
     6121 tcattttttt ctgcatacaa atacctcacc gaagacaccg ctactttcac cctccgcttc
     6181 ctgccggaaa agtcggacag ccagttctac gccatagccg tgaaaccgca aggcggcctg
     6241 ggccgcaccc tgtttatcaa ccgacggact tatctcgtgg acaagtcgca gcaaatccaa
     6301 gatggcgcgc tcatcaccac gacctacgcc gacttccgca gcgtggacgg cctcctgcag
     6361 ccgttccacc tgcaccagtc cagcggacag gtgatcttcg acaccgatat ccggattacc
     6421 agcgtggaaa caaacgtcgc cattgacccg gcggtctttg cccgccccgg cgcggcagcc
     6481 cggcgcgatt ttgctttcgc accgggcaag acctccacga ctgtaccctt tgaactggtg
     6541 aacaaccaca tctatttttc cgccaccctt caggggagcg tgaccggctc gttccttttg
     6601 gacaccggcg ccggagcggt ggtgctggat agcacctttg ccgccaagtc cggactcgtg
     6661 ggtgccggcg ggctggaagc caaaggtgtt tccggctcgg aaaaggcggg cttggtgaag
     6721 atcggctcac ttaccgtcgg cgacgtgacc ttcgacagtc tggtggcggc gactctcgat
     6781 ttacagccgt tgacgcaatt cgaaggccac ggcattgatg ggatcttggg atacgacata
     6841 ctggcccggg ttgtggtgga agtcgattac gagaagagcc aattgacctt ctccgacccc
     6901 gacctcttca cctacacggg cactggacag gtactgccta tgacgcttga ccagcgcatt
     6961 ccgcacgtgg aggctacagt tgaggggcag taccgcggct ggttcaacgt cgataccggc
     7021 tcgcgcaata ccctcgacct cttctcgcct ttcgtctttg aacacaccct ggcggacaaa
     7081 tatccgaaaa gagtcgaaac ccgcttaggg ggaattggcg acgatctgcc ctccaccacg
     7141 cagatggtgc ggatgaagtc ttttactgtg ggctcttttg aaattcccga cctgctcgtc
     7201 ggactctcaa cggcgcagaa agggatcttt gcctcggaga agttccaggg caacatcggc
     7261 agcgggcttt taaagcgctt tacggtgatc tttaactacc ccaaacagca actgatcctg
     7321 ttcccaaacc ggcacttcgg cgagcgcgac gagttcgaca aatcgggcct ctggctccgc
     7381 gaagtggcgg ggaaattcat cgtggaggaa gtggtcaagg gtactgcggg agataagtca
     7441 ggattcaagc ggggcgacgc aatcactgag atcaacggca agccggcgcg ggagtactcc
     7501 ttgaacgatc tgcgggccct cttccgcggc ccggacggca ccaagttttc gctcaaattc
     7561 cagcgaacca acaaagagaa gaaaaaagag ttgaaactga aaacgatttt gtagcctcct
     7621 cccccgctct gggaggcgcg actttagtcg cgcattcttt cgattagtag gtaaggttga
     7681 ccccagctgg cggaagtgta attatagatt tttccccttt tacccgcccc agtacccgcc
     7741 cgtacagcac cgttccttct ttgagcttta tgttccttgg gccgggactt ttgggacca
//
LOCUS       DBNGZA010000148         1841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2677940, whole genome shotgun sequence.
ACCESSION   DBNGZA010000148 DBNGZA010000000
VERSION     DBNGZA010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2677940"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16FU_03240"
     CDS             complement(<1..649)
                     /locus_tag="AC16FU_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR2993423.1"
                     /translation="MLVVMEVAATSEQIDAVCQVITEMGLKAHPMPGAQRTAIGITGN
                     IGVVDSSRIETLPGVINVIHVSQPFKLASREVKPEDTVVSVNGVKVGGQKLAIMAGPC
                     AVESREQALTIAKAVKKAGATIYRGGAFKPRTSPYSFQGLGGKGLEILAEVRKETGLQ
                     IVTEALDPEGVDLVEEHADIIQIGARNMQNFSLLRRAGRSRLPVLLKRGMSSTLEE"
     gene            complement(672..>1841)
                     /gene="argS"
                     /locus_tag="AC16FU_03245"
     CDS             complement(672..>1841)
                     /gene="argS"
                     /locus_tag="AC16FU_03245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR2993424.1"
                     /translation="GSEEALASDPIRHLYELYVRFNTEADANPATAEEGRRWFKKLES
                     GDSEAVALWQRFSRTSLDSFQKIYALLGISFAHFTGESFYNDRIEPTLKRIAEKKLSE
                     TSQEALIVNLEKYGMPPALLRKKDDTTLYATRDLAAAIYRYETFHFHRLLYVVGSAQR
                     LHFQQLFKVLELLGADWADRCVHVEFGWVKFEDKSMATRRGNLIFLEDVLDKAIALAE
                     KIIREKNPELENIDSVKRMVGVGAVIFADLAVRRQKDVNFEWEKVLTFEGETGPYLQY
                     THARLASLERKFSRTITAAINFELLTQKELWLLVRTLADFPQKVQIAAAQYEPAIVAG
                     YLLELAGRFNTYYQNYRIITEDPESTQAKMLVVAAARSVLQEGLRLLGLAAPERM"
BASE COUNT          360 a          568 c          492 g          421 t
ORIGIN      
        1 actcttccag cgtggaggac atgccgcgct tcaggagcac cgggaggcgg gagcggccgg
       61 cgcggcgcag cagggagaaa ttctgcatgt tgcgcgcgcc gatctggatg atgtcggcgt
      121 gctcctccac cagatcgacc ccttcgggat ccagcgcttc ggtgacgatc tgaagtccag
      181 tctccttccg cacctcggcc aagatctcca gcccttttcc ccctaatcct tgaaaactgt
      241 acggcgatgt ccgcggcttg aacgccccgc cccggtaaat cgtcgcaccg gcctttttca
      301 ccgctttggc aatagtcaac gcctgctcgc ggctctccac cgcgcacggt cccgccatga
      361 tcgccagttt ctgcccgcct actttaaccc cgttcacgga aacgaccgta tcttccggct
      421 tcacctcccg gctcgctaat ttgaacggtt gcgaaacgtg gatgacgttg atgaccccgg
      481 gcagcgtttc gatccgggac gaatcgacga caccgatatt gccggtgatg ccgatggcgg
      541 tccgctgcgc ccccggcatg ggatgggctt tcagccccat ctccgtgata acctggcaga
      601 cggcgtcgat ctgctctgac gttgccgcta cttccattac gactagcatt gatatcctcg
      661 attccgagta ttcacattct ctccggcgca gccaggccca aaagccgtaa gccttcctgc
      721 aacacgctgc gggccgccgc aaccaccaac atctttgcct gggtgctctc aggatcctcg
      781 gtgatgatgc gatagttctg atagtaggta ttgaaccgtc cggccagttc caggagatag
      841 cccgccacga tggccggctc gtactgcgcc gcggcgatct ggactttttg cggaaagtcg
      901 gccagcgtgc gcaccaaaag ccagagttcc ttctgggtga ggagttcgaa attgatggca
      961 gcggtaatgg tccggctgaa tttccgctcc agactcgcca gccgcgcgtg agtgtattga
     1021 agatatggac ctgtttctcc ttcaaaagtc aagaccttct cccattcgaa gttgacgtcc
     1081 ttttgccgac gcaccgccag atcggcgaaa atcaccgccc ccacccccac catccgcttc
     1141 accgaatcaa tgttttctaa ttccggattc ttttcgcgga tgattttctc cgccaaagca
     1201 attgctttat ccagcacgtc ctctaagaag atgagattcc cgcgccgggt ggccatactt
     1261 ttatcctcaa acttcaccca gccgaattcg acgtgtacgc agcggtcggc ccagtcggcg
     1321 cccagaagtt ccaagacctt gaagagctgc tgaaagtgca ggcgctgcgc cgatccgacc
     1381 acgtagagca gtcggtgaaa gtgaaatgtc tcgtatcgat agatcgccgc cgccagatcg
     1441 cgggtggcat acagggtcgt atcgtctttc ttgcgcaaaa gggccggcgg catcccgtac
     1501 ttttcaagat tgacgatgag cgcttcctgg ctggtttcgg agagtttttt ctcggcaatc
     1561 cgtttcaggg tgggctcaat ccggtcgttg tagaaactct cgccggtgaa gtgagcgaac
     1621 gagatgccta agagtgcgta gattttttga aacgaatcga gcgaagttcg gctgaacctt
     1681 tgccacagcg ccaccgcttc gctatcacct gattctaatt tcttgaacca ccgccgcccc
     1741 tcctcggctg tagccggatt ggcatcggct tcggtattga agcggacgta cagttcatag
     1801 agatgccgaa tcgggtcgct ggcgagcgct tcttctgaac c
//
LOCUS       DBNGZA010000149         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_239345, whole genome shotgun sequence.
ACCESSION   DBNGZA010000149 DBNGZA010000000
VERSION     DBNGZA010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_239345"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..>1978
                     /locus_tag="AC16FU_03250"
     CDS             536..>1978
                     /locus_tag="AC16FU_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2993425.1"
                     /translation="MSSLSQRVPVSPPVPEKLESKIPPTGLPDAILHEMEQTLQKIEA
                     ARPRLAGDKEGGKYRLADLEALLLINRVINSSLILDEILQIVMQKAIELLKAERGFLM
                     LVDERGELQFKTAHNIGKELLTDEDFRISRTIANQVARSGESVYTSDALKDDRYAHQK
                     SVMELHLRSIMCVPLKSRDAILGVLYLDNSSEVNVFLQSDLFLFELFAGQAAFAIEHA
                     KLYDSLLRMERYHEEVVNRTPVGIIVVDSAFTITTANEVAQQILGASGTLSGSLLEKK
                     IDLFTLFPSGEREKWRALCQKVLATGQAHAEPRYYLHAGPDERVLSLKISPLVRPETG
                     TVGLILVFEETTEKVMLENYVIMSEKLVAKGEMAAAIGHELNNYLTIISNCTEMLELT
                     VRKGDQEKLQQNTGVILENIGKMKRFTDGLMDYTRLETEPVPYELPRLVDDLLFAIKP
                     QQQFRKIRFVTEVAPGLPKVAMDVGQVQQVL"
BASE COUNT          461 a          583 c          563 g          371 t
ORIGIN      
        1 ccgtagctat agacgatact caaaatcggc caggcagtca ccacgaccaa tgcggtcgac
       61 gtcccaagcc gtgtctccgg ccacgggagc gtgatggcaa gaatatagct gaccacggcc
      121 aaaaccccgg caatcccgct cgccacccat agattacgat ttgacactat tcgcctctcc
      181 cgcaggctcc ggtgattgaa cttccatctg ccttgccaat aaaattacca tagccaatcc
      241 ccaaaaagtt gtcaaggtga tggatttcag caaatcgcct ccggcatgag gcgcaaaata
      301 cgccccttgg gattgtcgga caaaggatag agccgtccat ccggcccctg ccgcacggcg
      361 gtcttcccaa gtcggaattt aagtaactct cgatctggcc gatacttaaa gagatgagcg
      421 ggaaaccatt gctccgcatc tgacggggcg gtggtctctg cgcgttcatc tttacgtgca
      481 tagggcgcct atgagcaagt ccactgaaac agggaaagta aagaagcctt cgggtgtgag
      541 ctcgctttcc cagcgggtgc ccgtctctcc gccggtacct gagaaactcg agagcaaaat
      601 accgccgacc ggactgccag acgccattct gcacgaaatg gagcagacgc tccaaaagat
      661 tgaggcggcc cggccgcggc tggccggaga caaggagggc gggaagtacc gcttagccga
      721 cctcgaagca ctgcttttga tcaaccgggt catcaactcc tcgctcatct tagatgaaat
      781 cctccagatc gtgatgcaaa aggcgatcga actgctcaag gccgagcggg ggtttttgat
      841 gctcgtggac gagcgcgggg agttgcagtt caagaccgcc cacaacatcg gcaaggaact
      901 tttgaccgac gaggatttcc gaatttcccg caccatcgcc aaccaggtgg cccgctcagg
      961 cgagtcggtt tacacctcgg acgccttgaa ggacgaccgc tacgcccacc agaagagcgt
     1021 gatggaattg cacctgcgct ccatcatgtg cgtgcccttg aaaagccgcg acgccatcct
     1081 gggagtcctt tacctggaca acagttcgga agtgaacgtc ttcctgcaga gtgacctctt
     1141 ccttttcgaa ctctttgccg ggcaggcggc ttttgccatc gagcacgcca agctgtacga
     1201 ctcgcttctg cggatggaac gctaccacga agaagtggtg aaccgcacgc cggtggggat
     1261 catcgtggtg gacagcgcgt tcaccatcac cactgccaac gaagtggccc agcagattct
     1321 gggagcatcg ggaacactat ccgggtcgct tctggagaag aagatcgacc tgttcaccct
     1381 cttcccgtcc ggagaaaggg agaagtggcg ggcgctctgc cagaaggttc tggccacggg
     1441 gcaagcccac gccgagccgc gctactatct gcacgccggc cccgacgagc gggtcctctc
     1501 gctcaaaatt tcgcctctgg tgcggcccga aaccggcacg gtcggcttga ttttagtctt
     1561 tgaagaaacc accgaaaagg tgatgctgga aaactacgtc atcatgtccg agaaattggt
     1621 ggccaaaggg gagatggcgg cggccatcgg ccacgagttg aacaactacc tgaccatcat
     1681 ctccaactgc accgagatgc tggagctgac ggtcaggaaa ggtgatcagg aaaaactcca
     1741 gcagaacacc ggagtgatcc tggagaacat cgggaagatg aagcgcttca ccgacgggct
     1801 catggactac acccggctgg agaccgagcc ggtgccgtac gaactgccgc ggctggtgga
     1861 tgacctcctg ttcgccatca agccgcagca gcagttccga aagatccgtt tcgtcaccga
     1921 agtcgctccg gggttgccca aggtagcgat ggacgtgggg caggtccagc aggtgctt
//
LOCUS       DBNGZA010000150         3587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1087988, whole genome shotgun sequence.
ACCESSION   DBNGZA010000150 DBNGZA010000000
VERSION     DBNGZA010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1087988"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            589..1563
                     /locus_tag="AC16FU_03255"
     CDS             589..1563
                     /locus_tag="AC16FU_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993426.1"
                     /translation="MSKANGLAVEVKDLSRRFGSFVAVDRVSFAVERGEVFGFLGPNG
                     AGKTTTIRILCGILVPTSGSATVAGIDVAHKPDLVKEKIGYMSQKFSLYEDLTPAENL
                     DFFSGIYRISKKEREERKRWVLKMSGLVEWANEVTSELPVGFKQRLALGCAILHRPEV
                     VFLDEPTAGVDPASRRVFWKLIAELAAEGTTVFVTTHYLDEAEHCHRLALMNNGRIVA
                     QGSPQELKLSQRDQDFLEIECAEPVRALGLLRAAFPEWEIALFGNFLHVAAPVGDKAC
                     ASVRAFLQKAGVEIYRCEEIVPTLEDIFVTVVRREESVASVTEGKAPP"
     gene            1560..2696
                     /locus_tag="AC16FU_03260"
     CDS             1560..2696
                     /locus_tag="AC16FU_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993427.1"
                     /translation="MKSRIPAIMRKEFITVRRDSTVIANVFVLPLVMMLIYGYGIRFD
                     IRELPTAIYDLSRTPESRDLISAVVGSGYFRRVADLTSTAEVDTLFEQSKIKVALVIP
                     PRYATDVGLGKLARVQVLVDGSDANTATLAVGYLEGIIAKHSGSLLGEKFEKAGIHLP
                     FEIPAVTQETRLWYNPEQKSVNFIVPGIIVVMMSLLGALMTTMTIVGEKERGTMEQIV
                     ASPVTSTEFMVGKIVPYMLLAMTALALIVSCGYLVFGVPVKGSILLLTFFAFLFVTCA
                     LGLGLLVSTLASSLQVGMMLAMLITLLPSILLSGFIFPIESMPPVLQGFSRVVPGRYF
                     LEALRGIYLKGVGLGYLWPQALFLAGMGTLLLWVSTKKFRKRLD"
     gene            3001..>3587
                     /locus_tag="AC16FU_03265"
     CDS             3001..>3587
                     /locus_tag="AC16FU_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993428.1"
                     /translation="MFERTFHLVKKEVIQVLRDKRMLSILIIAPILQLALFGYVATTD
                     IRNLPMVVCDYDNSRWSREVAARFLNSGYFSLVGQVKSESEINAYLDGGKSKVALVIP
                     PDFGRKVAGGRKGEIGVALDGTNSNSAGIAANYIFSIVNRLSTDLVIQRLHTRGIKVE
                     QAAAVIPETRVWYNPELKSVNFMVPGIICVLLMMTT"
BASE COUNT          753 a          930 c         1118 g          786 t
ORIGIN      
        1 accgtccaga atatccggca ggtatcgcct tcgttagagg atgtgtttat ttcgctggcg
       61 aggcagaagt ctcaggtgga gtatggcggc agcagttgac ccctcccttt atccctcccc
      121 tcaaagggga gggaactata cctcttttgc agaagcagta gggttgagac gagtttccct
      181 ctcctttgag gagagggtgt cccgccaaag gcgggacggg agaggtcaga gtgcctcgtg
      241 agagaataga agttgacccc tcccttgttc cctcccctcg gaggggaggg tgtccgtacg
      301 gacgggagag gtcaggagac ataaagggaa agaaactgaa acatgagcgc agacaacgga
      361 tacgcagttg aagtttcgta gtcgaggatt ctatcctcgg cgcccgtacg ggcgtggcac
      421 ttcaaaaggg aacggttcca gcgaaccacc gaggttggtc ggtacgaccc gtatgggaaa
      481 cctcggctac gaagctaatt gtggggaagg ttggattcag gtcagtctcc ctctcctgta
      541 ggagagggta gggtgaggtc aaaagcatta aaggaaagat actgaaacat gagcaaagcc
      601 aacggactcg cagttgaagt caaagacctc tcgcgccgct tcggctcgtt cgtggcggtg
      661 gatcgggttt cgttcgccgt cgagcgggga gaagtgttcg gcttcctcgg ccccaacggc
      721 gcggggaaga cgacgaccat ccggatactg tgcgggattc tggtgcccac ctccgggtcg
      781 gccaccgtcg ccggcattga cgtggcgcat aagccggatt tagtcaagga aaaaatcggc
      841 tatatgtcgc agaaattctc tctctacgaa gacctgaccc ctgcagagaa tctcgacttc
      901 ttcagcggca tctaccgcat ctccaaaaaa gagcgcgagg aacgaaaacg ttgggttctc
      961 aaaatgtccg gtttggtcga gtgggcgaat gaagtaacct ccgaattgcc cgtgggattc
     1021 aagcagcgtt tggctctggg gtgcgccatt ctccaccggc cggaagtcgt tttcttggac
     1081 gagccgaccg ccggagttga ccccgcttcc cgccgcgttt tctggaaact gatcgccgaa
     1141 ttggcggctg aggggacgac ggtattcgtc accacccact acctggacga ggccgagcac
     1201 tgccaccggc tggctctgat gaacaacggc cggattgtgg cgcaggggag cccgcaggaa
     1261 ctgaagttgt cgcagcgcga tcaggatttt ctggagatcg aatgcgcgga gccggtgcgg
     1321 gcgctgggac tcctgcgcgc tgcttttccg gagtgggaaa ttgcgctctt tgggaacttc
     1381 ctgcacgtgg ccgcgcccgt aggggataaa gcgtgtgcca gtgtccgggc gtttttgcaa
     1441 aaagcagggg ttgagattta ccgctgcgag gagatcgtcc cgacgctcga agacattttc
     1501 gtcaccgtcg tccgacggga agagtcggta gcgtcagtca cggaggggaa agcgccgcca
     1561 tgaagtcgcg cattccggcc atcatgcgca aggagttcat cacggtcagg cgcgactcga
     1621 ccgtcatcgc caacgtcttc gttctgccat tggtgatgat gctgatttac ggctacggca
     1681 tccgcttcga tatccgagaa ctgccgaccg cgatttacga cttaagccgc acgccggaga
     1741 gccgcgacct gatttccgcc gtggtcggct cgggctactt ccggcgagtg gccgatttga
     1801 ccagtacggc ggaagtcgac accctctttg agcaatcaaa aatcaaagtg gcgctggtga
     1861 tcccgccgcg ctacgccacc gacgtcggat tggggaaact tgcgcgagtg caggttttag
     1921 tggatggttc ggatgcgaac acggcgactc tggcggtggg atacttagaa ggaatcatcg
     1981 ccaaacattc aggcagtctg ttaggcgaaa aatttgagaa ggcgggcatc cacctgccgt
     2041 ttgagattcc cgccgtcacc caagagacgc ggctctggta caatccggag caaaagtcgg
     2101 tcaacttcat cgtgccagga atcatcgtcg tgatgatgag tctcttgggg gcgctcatga
     2161 ccactatgac catcgtgggc gagaaggagc gggggacgat ggaacagatc gttgcctcgc
     2221 cggtcacctc aaccgaattc atggtcggga aaattgtccc ctatatgctg ctggcgatga
     2281 cggccttggc tttgatcgtc tcctgcggct acctcgtctt cggcgtgccg gtcaaagggt
     2341 cgattttact tttgaccttc ttcgcgtttc tgttcgtcac ctgcgcgctg ggtttggggc
     2401 ttctggtttc gactctggct tcttcgctcc aggtagggat gatgctcgcc atgctcatca
     2461 ctcttctgcc ttcgattttg ctctccggct tcatattccc catcgaaagc atgccgccgg
     2521 tgctgcaggg tttcagtcgc gtcgtcccgg ggcgctattt cttagaagcg ttacgcggaa
     2581 tttacctgaa aggggtgggg ttgggatacc tctggccgca ggcgctcttt ttggccggga
     2641 tggggacgct cctcctctgg gtcagcacga agaagttcag gaaacggctc gactgagtaa
     2701 ggtggaaggt ggtaggtcgt aggtgatagg ggataaacag gaattggggg ttgggatttg
     2761 ggagttaggg gaagcgggaa aagcagacac gggtagcata ggcccttgtg gccgttgcgg
     2821 agcaggtgat aggtggtggg tcgtaggtgg aagggaaaga caggaattgg gagtcgggag
     2881 ttaggattta gggaaaactt gaacgcgtag ggcgacccct acgggccgta tgaccgccct
     2941 cggtcgccta aggcagggaa tgagaacgaa ctcccgctgt attgtagctg aggaatgaga
     3001 atgtttgaac gcacgttcca tctggtaaaa aaagaagtca tccaggtttt gcgcgacaag
     3061 cggatgctct ccatcctcat catcgctccc attctccagt tagccctctt cggttacgtg
     3121 gccaccaccg acatccgtaa tctgcccatg gtcgtttgtg actacgacaa ctcccgctgg
     3181 tcgcgggaag tggcggcgcg ctttttgaac tccggctatt tctcgttggt cggacaggtc
     3241 aaaagcgaat ccgagataaa tgcctatctt gacggcggga agtcgaaggt ggcgctggtc
     3301 attccgcctg attttggccg caaggtggcc ggcggccgca aaggcgaaat cggcgtggcg
     3361 ctcgatggca ccaactccaa ttccgccggg attgcggcca actacatatt ttccatcgtc
     3421 aaccggctgt ccaccgattt agtgattcaa cgactgcata cccggggcat aaaagttgag
     3481 caggccgcgg cggtgatacc ggaaacccgc gtctggtaca acccggaatt gaaaagcgtc
     3541 aacttcatgg tgccggggat catctgcgtc cttttgatga tgaccac
//
LOCUS       DBNGZA010000151         5269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1088092, whole genome shotgun sequence.
ACCESSION   DBNGZA010000151 DBNGZA010000000
VERSION     DBNGZA010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1088092"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="AC16FU_03270"
     CDS             complement(<1..1018)
                     /locus_tag="AC16FU_03270"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2993429.1"
                     /translation="MNEAKQIVILGSGVGGLTCARRLREKLGRGHKIVLIDHRTEYVL
                     GASLLWIATGQRKPEEVTKNLSGVTRLDITFVPEEITRIDLQSRVVHTTSQEIGFDYL
                     VIALGAAFAMEKIPGLATAGFNLYTLPDAVRLHQALVDFPGGRIAVLVSALPFKCPAA
                     PHETAFLVDWFLTKRGVRPKTQIQLFTPEPLPMPAAGPEIGQVVKGMLVGRGVTPYFQ
                     HKATAVDPAKKEISFENGQRVQFDLLIIIPPHVSPRVVQEAQLTGDSGWIPVDRYTLR
                     TEFEGVYAIGDVTGIKIGSGFMLPKAAVFAQSQAEVVAHNIACQIKGSGNPYFFDGVG
                     ACYLE"
     gene            complement(1172..2128)
                     /gene="glpX"
                     /locus_tag="AC16FU_03275"
     CDS             complement(1172..2128)
                     /gene="glpX"
                     /locus_tag="AC16FU_03275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR2993430.1"
                     /translation="MDRNLALELVRVTEAAALSAARWMGKGDAMAADQAAVEAMRKAF
                     ASVEFDGTIVIGEGERDEAPMLFIGEKVGTGRSAQVDIALDPLECTNSVAYGRLNAIS
                     VIAAAERGNFLHAPDTYMEKIAVGPGAEGAIDLDLSPAENLRRIAEAKGYKVDDLTVV
                     ILDRPRHAELIKSVRQAGARIQLIPDGDVSAAIATALPDTGVDVLMGTGGAPEGVLAA
                     AALRCVGGAMQARLKPRNAAEIERAKKMGIRDPEKIYTEQELAGGTNIMFAATGITEG
                     DMLKGVRFFGGGAETHSLVMRCKTGTIRFITTRHSFDRKPIY"
     gene            complement(2198..3676)
                     /gene="rpoN"
                     /locus_tag="AC16FU_03280"
                     /pseudo
     CDS             complement(2198..3676)
                     /gene="rpoN"
                     /locus_tag="AC16FU_03280"
                     /inference="COORDINATES: protein motif:HMM:NF016439.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
     gene            complement(3836..4573)
                     /gene="lptB"
                     /locus_tag="AC16FU_03285"
     CDS             complement(3836..4573)
                     /gene="lptB"
                     /locus_tag="AC16FU_03285"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR2993431.1"
                     /translation="MNNHNQTLRSENLVKIYRNRRVVDGVSIDVKQGEVVGLLGPNGA
                     GKTTTFHMVIGFIQPNGGRVLIDDTDITDLPMYRRARRGIGYLAQEPSVFRKLTVEKN
                     ILAILEMQKLTHQERKERLKTLLAELDLTGLARRKAYTLSGGERRRVEITRALVNEPK
                     FLLLDEPFLGIDPIAVEEIQKIINRLKTRGLGILITDHNVRETLSITDRAYIMSDGEI
                     LKSGTAEFLASDPEARKIYLGEKFRLN"
     gene            complement(4611..4991)
                     /locus_tag="AC16FU_03290"
     CDS             complement(4611..4991)
                     /locus_tag="AC16FU_03290"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2993432.1"
                     /translation="MFTLVEAKVEFDGKRNVFKFFLQPIEKKAFRKERVVEHTRLVPT
                     HVKIAVWKRDYGSCVLCGAKENLHFDHEIPFSKGGSSITVQNVRLLCAQCNISKGSKI
                     MVFIPFLVSSLSVSRIDGLYSQVN"
     gene            complement(4979..>5269)
                     /locus_tag="AC16FU_03295"
     CDS             complement(4979..>5269)
                     /locus_tag="AC16FU_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993433.1"
                     /translation="KGYSILLMSVRKGAPYRDEFDQDTGLLIYEGHDVATKKTDPKSI
                     DQPMSFPSGKLTENGNFTKRQKISKREQHLVSVCKCMRKSYGEYGVTKECLR"
BASE COUNT         1041 a         1531 c         1469 g         1228 t
ORIGIN      
        1 tttccaggta gcaggcgccg acgccgtcga agaagtaggg gttgcctgag cctttgatct
       61 ggcaggcaat gttgtgggcc accacttcgg cctggctctg ggcaaaaacc gcggccttgg
      121 ggagcataaa gccggagccg attttgatgc cggtgacgtc gccgatggcg taaactccct
      181 cgaattcagt ccgaagggtg tagcggtcaa ccggtatcca accggagtca ccggtcagtt
      241 gtgcctcttg caccacacgg ggagaaacgt gcggagggat gataattaaa agatcgaact
      301 gaaccctctg tccgttttca aaggagattt ctttttttgc cgggtcaacg gcggtggcct
      361 tgtgctggaa gtagggcgtt accccgcggc ctacaagcat ccccttaacc acctgcccta
      421 tttccggtcc ggcggcgggc atgggcaaag gttccggggt gaaaagctgg atttgggtct
      481 ttgggcgcac cccccgcttg gtcagaaacc aatcgaccaa aaacgccgtc tcgtgcggcg
      541 ccgccggaca cttgaagggg agcgcggaaa cgaggacggc aatgcgcccg cccggaaaat
      601 caaccagcgc ttggtgcagg cggacggcat cgggcaaggt gtagagattg aatcccgcgg
      661 tcgccagccc gggaattttc tccatggcaa aagcagcgcc cagggcaatg accagatagt
      721 caaaaccgat ttcctgggag gtggtgtgga caacgcggga ttgcaggtca attctggtga
      781 tttcttccgg aacgaaggtg atgtcgagac gggtcacccc ggagagattt ttggtcacct
      841 cctccggttt ccgctggccg gtggcaatcc acagcagcga ggcacctaag acatactcgg
      901 tccggtggtc gatgaggaca atcttatgcc cgcgacccag tttttcccgc agacgccggg
      961 cgcaggtgag cccgcccacg ccgctcccga ggatgacgat ttgcttcgct tcattcattc
     1021 gcagacttaa ataacatcaa caagtggcca ttgtaaatcc gggtaaaaag cctttctgga
     1081 tgaagggcgg cactgaaagt gccgcctgcg cggagagcgg gatgttcttt tcgcgcggat
     1141 aagtggtccg acgcctgtcg aaaacggtcg ttcaataaat cggcttgcgg tcgaacgaat
     1201 gccgcgtggt gatgaagcgg atggtgccgg tcttgcagcg catgaccagc gaatgggtct
     1261 ccgccccgcc gccgaagaag cgcacccctt tgagcatgtc gccttcggtg atgccggtgg
     1321 cggcgaacat aatgttcgtc ccgcccgcca attcctgttc ggtatagatt ttttccggat
     1381 cgcggatgcc catttttttg gcgcgttcaa tttctgccgc gttgcgcggt ttgagccgcg
     1441 cctgcatcgc cccccccacg cagcgcaacg ccgcagcggc caatacccct tccggcgcgc
     1501 cgcccgtgcc catcaggacg tccaccccgg tgtcggggag ggccgtggca atcgccgccg
     1561 aaacgtcgcc atccggaatc aactgaattc gcgccccggc ctggcgcacg ctcttgatca
     1621 actcggcgtg cctcggtcga tccagaatca ccaccgtcag gtcatcgact ttgtacccct
     1681 tggcctcggc aatccggcgg aggttttccg ccggcgaaag atcgagatca atggccccct
     1741 ccgctcccgg cccgaccgca attttctcca tgtaggtgtc cggcgcgtgg aggaagttcc
     1801 cccgttccgc ggcggcgatc accgaaatgg cgttcagccg tccgtaggcg acgctgttgg
     1861 tgcactccaa aggatcgagc gcaatatcga cctgggccga gcggccggtg cccacctttt
     1921 cgccgatgaa aagcattggc gcctcatccc gctctccttc gccgatgacg atagtgccgt
     1981 cgaattcgac cgaagcgaag gctttgcgca tggcttcgac cgcggcctgg tcggcagcca
     2041 tggcatcccc tttgcccatc cagcgggcag ccgaaagcgc cgccgcttcg gtaacgcgca
     2101 ccagttccag cgccaggttt cgatccattt cagttggaat ttagccaaaa gacaaaaaaa
     2161 accaagccgg gggcttggcg gtgcgagtta ggagcaatta tgacgactgc ccgaatgaat
     2221 tcggggtagg gccgggggca acggggagcg gagtatcgcc gttggctttc accgaccgtt
     2281 tgcggaaacg ggccgcctgg atgttcaatt cttcgcggta cttggtcacc gtgcggcggg
     2341 cgagcgtcaa cctctcggct ttcagtttct caaatatctc ctgatcggag agcggctgcg
     2401 cgggattctc ctcccggatc agtttggtaa tccgctcctt gaccgtttgc ttggaaatgt
     2461 cttcgccgcc cacctgcggc accccctggt tgaaaaagcg gcggatttca aagactccaa
     2521 agggcgtctg cacgtacttg tcgttggcca cccgggagac ggtggccacg ttcatcccga
     2581 tccggtcggc cacctcttac atggtcaggg gtttcaagtg ctccggcccg tgttcaaaga
     2641 agtttttttg ggtttccacc acggcgttca tcaccttgat catcgtgttg cgcctttggt
     2701 tgatggcgtt caaaagccag cgcgcctgct ccaatttctc ccgcacgtag cttttggtct
     2761 cggcggcgga agacgagttg cgccggagca tctcccgata ggcggggttg atgcgcaggc
     2821 gcgggatgtt ccggtcgttg tggtagacga cgaactcctc gccaatccgc tccacggtta
     2881 aatccggcac caccggctgg gccgcggtga cgaagcggcc ggtcgcgggt cggggggaga
     2941 gggtgcggat caccgccatc gcttcttcca cccgttccag ttccacgccc aaaagccgcg
     3001 ccagctggga gttgctcttc cggtcgagat cggtgagctg ctcggcgacg atttgccagg
     3061 gcagggtatc ttccagaccc cgttcgcgca tttgaatcaa aagcgcctcc cggaggtcgc
     3121 gggcccccac ccccaccgga tcgaatcctt gaatgacctt caaaactttc tccaccgcgg
     3181 ccaccggcac ctggagcgtc tcggctattt cctccaccga aatcgtcagg aaaccgctct
     3241 ggtcgatgtt gccaataata aagaggccga tctgccgctc ctcttcggtg acccggtgga
     3301 gggagagctg gtcggctaag tgttcgtaga gggttttttc ggaaacggga gtctggaggg
     3361 gttcgtcctg atctttttcc cgatgggcgg cgatggcgta gtcggtggcc ttggcgtctt
     3421 cgtctaagta attgtcccaa tcgatcttgt tcagctccgg gtcattggga ccgtcggctg
     3481 attccgtttc gggggtggcc gtgtcggttt ccggttcttc ctccaaggcc tcggcttcct
     3541 ccaggagcgg attggttgac agctcgaccc gcagcagttg ctccaatttc aagatcggca
     3601 cctgcagcaa atgcagagac tgaatcaact ggggggcgat ggtttgcctg agttttgcct
     3661 ggagtcctaa cttcataatt gactcttcac cgcctggtct gcgtccctac tgcgatcccg
     3721 ccgtgaccgg ggcttgacct cagaatagcc gcccccggcc tgcgcaaaaa ttgcacagcc
     3781 ctcgaccctg gcgttggttc cgggtactga ttcagttgtc ggcggaaaga gagccctaat
     3841 ttaaccgaaa cttttcgcca aggtagatct tgcgcgcctc cgggtcgctg gccaggaact
     3901 cggccgtgcc tgacttcaaa atctcaccat cactcataat atacgcacgg tcggtaatag
     3961 acaaagtctc ccggacgttg tggtcggtga tgaggatgcc caaccccctg gttttcagcc
     4021 ggttaataat tttttgaatt tcttcgacgg cgatcgggtc gatgcccaga aacggctcat
     4081 ccaaaagcag gaacttgggc tcgttgacca gagcccgggt gatctccacc cgccgcctct
     4141 ccccgccgga gagggtgtag gccttgcgcc gggctaagcc ggtgaggtca agttcggcca
     4201 ggagggtttt gagcctttcc ttccgctcct ggtgggtcag tttctgcatt tccagaattg
     4261 ccaggatgtt cttttcgacg gtcagtttgc ggaaaaccga cggctcctgc gccaggtagc
     4321 cgatcccccg ccgggctctt cgatacatcg gcaggtcggt gatgtcggtg tcgtcgatca
     4381 acaccctgcc gccattgggt tggatgaaac cgatgaccat atgaaaggtg gtagtcttgc
     4441 cggcgccgtt cgggcccaaa agccccacca cctccccctg cttcacatca atagacaccc
     4501 catccaccac ccgacggttg cggtagattt tgaccaaatt ctcggagcgg agggtttggt
     4561 tatggttgtt catagttagt gttagctatt tccagttacc cctgtgtact ttagtttact
     4621 tgagaatata ggccatcaat tcggcttacc gagagtgaac tcaccaagaa tggaataaat
     4681 accatgatct tgcttccctt gctaatatta cattgggcac aaagtaagcg cacgttctgt
     4741 acagtaatac tacttccgcc ttttgaaaaa ggtatttcat gatcgaagtg taaattttct
     4801 tttgctccac acaacacgca cgacccataa tctcttttcc aaactgctat cttaacatga
     4861 gttggtacaa ggcgagtatg ttctactact ctttctttcc tgaaggcctt cttctcaatc
     4921 ggttgaagaa aaaatttgaa aacattccgt tttccatcaa attctacttt cgcttcaact
     4981 aacgtaaaca ttcctttgtc actccatact ccccgtaaga ttttctcata cacttgcaca
     5041 cgctcaccag gtgctgctcc cgttttgaaa tcttctgccg ctttgtaaaa tttccatttt
     5101 cggtaagctt cccactcggg aaactcatag gttgatctat agacttcggg tcggttttct
     5161 ttgtggcaac gtcatgtcct tcataaataa gcaaaccggt atcctggtca aattcgtctc
     5221 gatatggagc tcccttccga actgacatta aaagaattga atatccttt
//
LOCUS       DBNGZA010000152         2621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_239721, whole genome shotgun sequence.
ACCESSION   DBNGZA010000152 DBNGZA010000000
VERSION     DBNGZA010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_239721"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1911)
                     /gene="selB"
                     /locus_tag="AC16FU_03300"
     CDS             complement(34..1911)
                     /gene="selB"
                     /locus_tag="AC16FU_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR2993434.1"
                     /translation="MFVLGTAGHVDHGKSTLVTRLTGIDPDRLPEEKARGMTIDLGFA
                     WLALPSGLEVGIVDVPGHERFVRNMIAGVGGIDAVLFVVAADDGWMPQSAEHLAILKL
                     LGIQRGLIVLTKIDLVKPDWRILVEEDIREKLKGSFLAQAPLVKVSAPTGEGVETLKQ
                     EIGSLVARLAPRRDLGKPRLFIDRVFSMAGRGTVVTGTLRDGSLRVGEEIEILPTGLR
                     ARIRGLETHKKPVEQSGPGARLALNLSGVDKEKLSRGDLIIKPGQGFLTRRWAASVAL
                     WEQSPFPLKDNSEVLFLLGTSETRAKVHLLDRSVAQPGEEVLVEFTAPSPVAARIGDH
                     FILRLPSPGVTLAGGKVLELEPFWRKRRTPEVRAYLSERVSLAASDLLRSELEKSVVQ
                     PRTTLLFHSHIAPAEIEAALLDLERQQLLWQWGGFVGLRQKIEGLQREIVTLVEKEHQ
                     THPYRLGIVRAEMAGRLRKNEAEIDPLIVQMLEKGLLVQEAGFLRLPPFSPRLLPEQE
                     RQVEKLLNEFEQSPYGAPTKEEIFARGDGWKDLVAFLLYQKTLIDCGDGVLLRALDWE
                     AVTRKVTAFFKENGTMTVGQFRDLLHTSRKYAVPILETLDHVGLTRRVGDQRVLVDTH
                     D"
     gene            complement(1921..2607)
                     /locus_tag="AC16FU_03305"
     CDS             complement(1921..2607)
                     /locus_tag="AC16FU_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2993435.1"
                     /translation="MGFVRSVSVRDLVLLGQEHGVPVMEDIGSGAVPDTAEFGLKTEP
                     TVGGAVSAGCNVVSFSGDKLFGGPQAGILVGKKEIVERLKKHPLYRALRVDKIIFAAI
                     EVLARQYLTKATDKIPVWQMITLPIDTLQKRAQVLAKELTGDKITVRVVECRSTVGGG
                     SLPGETIPSSGLEFESEVSPHEIAAVFRGQSPPVIGRIENDRFLIDLRTVFPDQDKSL
                     VTAAQQVFKK"
BASE COUNT          559 a          768 c          752 g          542 t
ORIGIN      
        1 gatggaaaaa atatttgcgg tagaacgaaa agctcagtcg tgggtgtcga ccagaacgcg
       61 ctgatccccc acccgccggg tgaggcctac gtgatctaat gtttccagaa tcggcacagc
      121 atatttgcgc gaggtgtgca gaagatcgcg gaactggccc acggtcatcg tgccattttc
      181 tttgaaaaat gccgtcactt ttcgggtgac tgcttcccag tcaagggcgc gtagcaacac
      241 gccgtcgccg cagtcgatta aggtcttctg gtaaagcagg aaagccacca gatctttcca
      301 cccgtcgccg cgggcgaaaa tctcttcttt ggtgggcgcg ccgtaaggtg attgttcaaa
      361 ttcgtttaat agcttttcga cctgtcgttc ctgttccggc aaaagacgcg gggaaaaggg
      421 gggcaaacgg aggaaaccgg cctcctgaac gagcagacct ttttccagca tttggacaat
      481 cagcggatcg atctccgctt cgttcttccg cagccgaccg gccatctcag cccggacaat
      541 accgagccgg taagggtggg tttggtgctc tttctccacc agggtgacga tttcacgctg
      601 aagcccttca attttttggc ggagaccgac gaagcctccc cactgccaga ggagttgctg
      661 tcgctctaaa tccagaagcg ccgcctcgat ttcagccggg gcgatgtggc tgtgaaagag
      721 caaagtggtg cgcggttgca cgacactttt ttccaactcg gaacggagga gatcgcttgc
      781 cgccagcgaa acacgttccg atagatacgc ccgaacttcc ggtgtacgcc gtttgcgcca
      841 gaaaggttcg agttccagca ccttgccgcc agccagggta actcccggcg agggcagccg
      901 caggatgaag tgatcgccga ttcgagcggc cactggtgag ggagcggtaa actcaactaa
      961 aacctcctca cccggctggg caacgctccg gtcgaggagg tgaactttgg ctcgcgtttc
     1021 gctcgtgccc aaaaggaaga gcacctcgct attgtctttg agcggaaacg ggctttgctc
     1081 ccagagcgca accgacgcgg cccagcgccg ggttaaaaag ccctggcctg gtttgataat
     1141 caaatctccc cgggacaatt tttctttgtc aacgcccgag aggttgagcg ccagccgtgc
     1201 gccggggccg ctttgctcca cgggcttctt gtgggtttct aaaccgcgaa tccgcgcgcg
     1261 caaaccggtg gggagaattt cgatttcttc gccaaccctg agggaaccgt cgcgcaacgt
     1321 gccggtgacc accgtcccgc gcccggccat gctgaagacg cggtcgatga atagccgcgg
     1381 cttgccgaga tcgcgccggg gcgccaggcg cgccaccagg gaaccgatct cctgctttaa
     1441 ggtttccacc ccttcaccgg tgggagccga gacttttacc agaggagcct gagcgagaaa
     1501 acttcctttg agtttctccc ggatgtcttc ttccaccaaa atgcgccagt ccggttttac
     1561 cagatcgatt ttcgtgagga ctatcagccc ccgctggatc cccagaagtt tcagaatcgc
     1621 cagatgttcg gcggactggg gcatccagcc gtcatcggcg gccaccacga agagtacggc
     1681 gtcgatgccc cccaccccgg caatcatatt ccggacaaaa cgctcgtggc cggggacgtc
     1741 gacgatcccc acttccagac cggaaggaag cgccagccag gcgaagccca ggtcaatcgt
     1801 catcccgcgg gctttctctt ccggcaggcg gtcggggtcg atgccggtga ggcgtgtaac
     1861 cagtgttgac ttgccgtggt ccacgtgccc ggcggtgcct aagacgaaca taagacctat
     1921 ctattttttg aagacttgct gagcggcggt gacgagactt ttatcttggt cagggaaaac
     1981 cgtccggagg tcgatgagaa aacggtcatt ttcaatacgg ccgatcaccg ggggagattg
     2041 cccgcgaaac acagctgcaa tctcatgcgg ggaaacttcg ctctcaaatt caagaccgga
     2101 ggaaggaatg gtttcaccgg gcagtgatcc gccacccacg gtgctgcggc actcaacaac
     2161 ccgaacagtg atcttgtctc cggtcagttc cttagccagt acctgagcgc gtttttggag
     2221 agtgtcaata ggcagtgtga tcatctgcca gaccggaatc ttgtcggtgg ctttggtgag
     2281 atactggcgc gcaagcactt caatggccgc aaagatgatt ttatcgacgc gcaaggctcg
     2341 gtagagcgga tgttttttca accgttccac gatttctttt ttaccgacga gaatccccgc
     2401 ctgcgggccg ccgaacagtt tatcgccgct gaaactgact acattacagc cagcggaaac
     2461 ggccccgccg acggtgggct cggttttcag tccaaactca gcggtgtccg gaaccgcgcc
     2521 ggagccgata tcctccatta ccggaacccc gtgctcctga cctaagagca ccaggtcacg
     2581 gacggagacc gagcggacga agcccacctg caggaaattg c
//
LOCUS       DBNGZA010000153         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2890606, whole genome shotgun sequence.
ACCESSION   DBNGZA010000153 DBNGZA010000000
VERSION     DBNGZA010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2890606"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..767)
                     /gene="lexA"
                     /locus_tag="AC16FU_03310"
     CDS             complement(147..767)
                     /gene="lexA"
                     /locus_tag="AC16FU_03310"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR2993436.1"
                     /translation="MEKNVRPLTKRQKQILDFIDSTIQKAGHPPTLREMGKRFGIKST
                     NGVRTFLIALEKKGYIKKAPYRSRGIYMADQLKAAWRPVPVVGKIAAGLPITAIENIE
                     SSIAIDKTFLPGDGVFSLKVTGESMKNAGIFDGDYVLVQQQATANPGDIVVAIIGEEA
                     TVKRFYPEKGHVRLMPENPDFSPLIVEKNTPGFSLAGKVVGVMRRL"
     gene            complement(909..>1667)
                     /locus_tag="AC16FU_03315"
     CDS             complement(909..>1667)
                     /locus_tag="AC16FU_03315"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2993437.1"
                     /translation="PEYLLQVAVLDTQGLVVKDYGQKAFAKPGSTAVLASSIPIDSLP
                     EGKYLLDLTVVDRATGQVAEQRKRFTVVGAVTLPQTLTFADSGAFREEEAKAAKSLIS
                     YLATPEELLQYDRLPLGEKYSFIQNFWKKRDPDPNTRINEFLTEYYRRFLYANANFSA
                     DLKEKKDGWKTDLGRVYMVYGPPNDIERGYMASDQAAWERWQYSSLPGQGGVYFLFVE
                     EHDTGRWRLVDSTARREIHDPRWEERIQTERLQP"
BASE COUNT          337 a          493 c          427 g          410 t
ORIGIN      
        1 acacggtgcg tctaccccat tttgattatt ccttgtgctc tacctaaact acgtcaaaag
       61 cacagaaagt gcatgctttt agaaaaagtc tcttcatcgg tctttttctc tccctcagtt
      121 ttcaatcctc aatcctgtct tttcttttag agccgccgca tcacccccac caccttcccc
      181 gcaagggaga atcccggcgt gttcttctcg acaatgagcg gcgaaaaatc aggattttcc
      241 ggcatcaggc ggacgtggcc tttctccgga tagaagcgct tgaccgtcgc ctcttccccg
      301 ataatcgcca ccacgatgtc ccccggattg gcagtggcct gctgctgaac taagacgtag
      361 tccccgtcaa aaatccctgc gtttttcata ctttcgccgg tgactttgag cgaaaagacg
      421 ccgtcacccg gcaggaaggt tttatctatc gcgatactcg attcgatatt ttcgatggcc
      481 gtaatcggca gtcctgccgc tatcttcccc accaccggca ccggccgcca ggcggccttc
      541 aattggtcgg ccatataaat cccccgcgag cggtagggcg ctttcttgat gtaccctttc
      601 ttctccaggg cgatgaggaa agtccgcacg ccgttggtcg atttgatccc gaagcgtttg
      661 cccatctccc gcaaggtcgg cggatggccg gctttttgaa tagtgctgtc aatgaaatcc
      721 aaaatttgct tctgtctttt ggtcaacggc cgaacatttt tctccattac catataacct
      781 cctttttatc cccggccaca accttaaagt ctacgtcggg caagctacac atccgtatat
      841 actgaacaaa agaatagctg tcaagagaaa aaatcactct ctggtggctt acgaatttcc
      901 ggagtgcctt acggctggag ccgttcggtc tggatacgtt cttcccagcg cgggtcgtga
      961 atctcccgcc gggcggtgga atcgactaag cgccagcgac cggtgtcatg ctcttccaca
     1021 aagaggaaat agacgccgcc ctgccccggc aggctggagt actgccagcg ctcccaggcc
     1081 gcctggtcgc tcgccatgta gccccgttcg atgtcgttgg gcgggccgta gaccatgtag
     1141 acccggccca ggtcggtctt ccagccgtct ttcttttctt tcaggtcggc ggagaagttg
     1201 gcgttggcat agaggaagcg ccggtaatac tcggtcagga actcgtttat ccgggtgttc
     1261 ggatccgggt cgcgcttttt ccagaaattt tgaatgaagg aatacttttc acccaggggc
     1321 aggcggtcgt actgcaaaag ctcttcgggc gtggcgagat aggaaatgag acttttcgcc
     1381 gctttcgctt cctcctctcg aaaagccccg ctgtcggcga atgtgagggt ctgcggcagg
     1441 gtcaccgcac cgaccaccgt gaaccgtttc ctttgttcgg caacctgacc ggtggcccgg
     1501 tccaccacgg tgagatcgag gagatacttg ccctccggca gactatcgat cggaatggaa
     1561 ctggccaaaa cggcggtgga acccggcttg gcaaacgctt tctggccgta gtcttttacc
     1621 actaagccct gcgtgtcgag cacggccacc tgcagcaggt attcagg
//
LOCUS       DBNGZA010000154         1631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1407638, whole genome shotgun sequence.
ACCESSION   DBNGZA010000154 DBNGZA010000000
VERSION     DBNGZA010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1407638"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..1252)
                     /locus_tag="AC16FU_03320"
     CDS             complement(413..1252)
                     /locus_tag="AC16FU_03320"
                     /inference="COORDINATES: protein motif:HMM:NF039484.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993438.1"
                     /translation="MAPKKGYATVNRIFPGRYDPAKGGEQSREMGRDDPYGVTRKAVF
                     DLEFVWKAGWAVASGYLLVLCQISEKFSPLVWVALVPLLFVLRRADTKQALWYGFLWG
                     VVYFGLANLAVTLKGSVVVTHSEHFTTFASTGGSAHVISGWLLAFLCAFGFSLTTLFI
                     STLSRRALFNPILIASVWAAFELVRIKLGVAFGLLADAHHSPFLLNFANFAGLIAVSF
                     LIALANSAWVSLYEWVREKFTPAYRFASLPDFPHILIPNSQIRYQILALVPEVRGPPG
                     AAI"
BASE COUNT          413 a          472 c          420 g          326 t
ORIGIN      
        1 gcacttcgca aacttcccac gtaacggcgt aattcccccc tcgtccctgc gttataacac
       61 tatccaccgc agtaccgtcg gcgaccacga tggagcctac aacttgcccc tcggcattac
      121 gacaagcaac acttatcgta cctcctgttc tattcctgat ttttaactta tctcccggct
      181 cttgcttgta gggagggggg ggcgttagga caagagttgg gggcggtccc gtgtctttcg
      241 tgactgtaac tgttacatcc ccagtaggcg ccggtattgc cactggagta aaggcatagg
      301 tcgctaaaaa cattactaaa agaccaatca ccacgttgct tgctctagat actcgcattg
      361 caactcctcc ttgaaaagat gtgtggttac tactccactc accgtaccaa ccttatatag
      421 ccgcacccgg cggcccccgc acctccggca ccagcgctaa aatctgatat cggatttggg
      481 agttaggaat taggatatgg ggaaaatctg gcaaagaagc aaagcgatag gcgggtgtaa
      541 acttttctct aacccactcg tagagcgaaa cccaggcgct gttggccagg gcaatgagga
      601 acgaaacggc tataagcccg gcaaaattgg caaaattgag caggaatggg gagtggtgtg
      661 catcagccag aagaccgaag gcgactccta attttatccg caccaattca aacgccgccc
      721 acaccgaggc aatgagaatg ggattgaaga gcgcgcgccg tgaaagagtg gagataaaaa
      781 gggtcgtcag actgaacccg aaggcgcaca agaaagccaa aagccacccg gaaataacgt
      841 gggcggagcc gccagtagag gcaaaagtgg taaagtgttc ggaatgagtg acaaccaccg
      901 aacccttgag cgtgaccgcc agattcgcca gcccgaagta aaccaccccc cagaggaacc
      961 cgtaccagag cgcctgcttg gtatccgccc ggcgcagaac aaaaaggagt ggcaccaaag
     1021 ccacccagac taaaggacta aatttctcgg aaatctggca aaggactaac aggtacccgc
     1081 tggccactgc ccagccggct ttccagacaa actcaagatc aaatactgct tttctcgtta
     1141 ccccatacgg gtcatcccga cccatctccc ggctctgttc cccgcccttg gcggggtcgt
     1201 accgaccggg gaaaatccga tttaccgtag catagccctt tttaggcgcc aaacgcggac
     1261 aagccgctat gctacacgtc cactttccaa tctgtgccga ctaaagcagt gctcttcgtc
     1321 ttcattcggg tcgtagaccc tgagcgaagc cgaagggtgg ggtctgccgc tttagccctc
     1381 cctcctcagc gggggagagt ataattcagc ggcgtcaact tgtcaaggat tttcttatct
     1441 taaagggata gatttactcc tgtatccccc ggccaccaac catccgcggg gcacgcttcc
     1501 ggagaaaccg ctgtcaggga taaggtagtc gggctcgtct ccgagcccga catttcctcg
     1561 gtacgagccc tcgccctgag ctcgggccga agggtcattc cgatcgcaga gagacgagcg
     1621 ggagtaccgg t
//
LOCUS       DBNGZA010000155         5532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_240024, whole genome shotgun sequence.
ACCESSION   DBNGZA010000155 DBNGZA010000000
VERSION     DBNGZA010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_240024"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1900)
                     /gene="dnaG"
                     /locus_tag="AC16FU_03325"
     CDS             complement(95..1900)
                     /gene="dnaG"
                     /locus_tag="AC16FU_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR2993439.1"
                     /translation="MSSQNQFDRSDTIDRVRDASDIVEVIGQYVTLKKRGNSYLALCP
                     FHSEKTPSFSVSQQKQVYHCFGCGKGGNVFTFLTEHEKMTFGEALRYLARRANIELPR
                     TKSPQGQKESEDLYRATQVALAYFRESFADPRLGKEGKAYLRERELSAETIEEFQVGY
                     APFAWDGLIAFAAERGVPAEMLFKVGLVARKDATAYSGGSRYYDLFRRRVVFPIFNLS
                     NAPIAFGGRVINTEDQPKYLNSPEHPLYQKGKTLFGLNLSKEEIRSTGKAVVVEGYLD
                     LISVYQVGIKNVVAPLGTAFTPDQARLLARFASEAILFFDPDAAGQSAVLRSIEHLID
                     QGFSVRVALLPAGEDPDTFARKHGQSWIQEVLQEAVGFLEYKKSRLEADFTTLSFSRK
                     EEVLQDLALLASRVADPARRNLFLSEAGDTFGVELAVLLGYVKKLRRQSARPPASRPE
                     AEKAPRAAPHPGDSRERELLGLVFASADVLKDAAGFMHRSDWQNPALADLFELAVKHF
                     HQEGKVSLSGLLDSAGVEEKRQVLVKLSDWEAPDAELSWQMWQDHRKLVLKRRREEQI
                     RLLSGQIEVAQKQGHEEEAVRLTRKLREVSRAIAG"
     gene            complement(1913..4300)
                     /locus_tag="AC16FU_03330"
     CDS             complement(1913..4300)
                     /locus_tag="AC16FU_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR2993440.1"
                     /translation="MDRHSLEVLEFDKIVALVGERCLAAPGRELAGALTPQTDPGRIA
                     TWLSETAEVKEIITTDQHFPLRSYPDIREHLATVKMEGTFLEPLRLLEVADFSGLVGD
                     LVRTAKALLNAYPLVTSHLAGLTAHSELKKAIEKAIDPSGEIRDSASPALHTLRQQKQ
                     KIRREILAKLEAILSRRKTPQAQVTLRDNRYVIPVDEHDLSRQKGIVHDRSASGATFF
                     VEPLETVELNNRLRETEIKERQEIDRILRALSDHVRKHQDELAENLEILAQLDFLRAK
                     ARLSVDLNGSAPKITAGAFVSLLNARHPLLLLQVKTQPGKQVIPLDFSLGRDFSCVIV
                     TGPNTGGKTVALKTLGILVLMAQAGLHIPASPDSEIGLFGSVWADIGDEQSIEQSLST
                     FSSHMSRIIQALSRADAATLVLFDELGVGTDPKEGAALGEAVLLDLLEKKAKIVATTH
                     YSALKTLAQQYPQIRNASLEFDRKTLRPTYRFSLGLPGASYALEIAERLGMPKKVTNK
                     AAELVGSQERSLVKLLEEVEENLHALSEDRKQVALQKATVGELERFIAEKKKKLDERE
                     RQAKAAALEEAQEIVRTTRKETERLVAEIRRSQADKKVVKETQTSLQRTAEELAAKGK
                     ELAPVVTPEMTELRIGDTVSVALSTDGAQQISGEVVELANDERKVRLWVGNIFYTVYR
                     EQIVKKVEPSAVPSSSTPPRRQLEEEKGVSRELSVRGMTVDEALAEIDKYLDGVALSG
                     LSEVVIIHGKGTGKLRKEVGAYLKNHPLVAATRLGQWNEGGEGVTVVQMKREGKL"
     gene            4531..>5532
                     /locus_tag="AC16FU_03335"
     CDS             4531..>5532
                     /locus_tag="AC16FU_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR2993441.1"
                     /translation="MERMRPGVLVLTFMFLFSSLFLLSSVPARGENAALPAKGDESNS
                     PDSARMPVYQLDGIVVTANRYEKSAFAVPYAMSILSADKFQQAGAPIIADLLRNLAGV
                     DVTDAGPFRTRPIIRGLAGSRILVLVDGQRMNDTRENTFSGAELSQVSPGQVERVEVL
                     RGSNSVLYGSNAMGGIINILTRQPSARRGPLAVSGSLVNRYSTTDQQPYSRLNLNLAN
                     HRWRLAGGAEYRRANNYHAPGRSVFGRGREIQNSNLRRSVGLDFAGSYRLADRHTLAL
                     EAQSMLNSEIGFPETPNPTFPVGIFFPYHDRSKIALTYEGKKITPRLVSIRATLYGQK
                     "
BASE COUNT         1075 a         1686 c         1549 g         1222 t
ORIGIN      
        1 ctgagctatg ggccctggtc gagttaaaga aagccgaatg ttatcagtcc gtcaaggcag
       61 ggcgcatgcg gcgaaccttg ccgcagtaac cgcgttaccc ggcgatggcc cgagaaactt
      121 ctcgtagctt tcgagtaagc cgtaccgctt cctcttcatg tccctgtttt tgggcaactt
      181 ctatctgtcc actcaacaac cgtatctgct cttcccgccg gcgcttcaaa accaatttcc
      241 ggtgatcctg ccacatttgc caggagagtt ctgcatccgg agcctcccag tcactcaatt
      301 tcactaacac ctgtcgcttt tcttcaactc cggcagaatc gagcaagccg gatagcgaga
      361 ccttcccctc ctggtgaaag tgcttcaccg ccagctcaaa caggtcagcc agagccgggt
      421 tctgccagtc gctgcggtgc ataaacccgg cggcatcctt gagcacgtcc gcggaagcaa
      481 agaccagacc cagcagttcc cgctcccggc tgtcacccgg gtgcggggcg gctcttggcg
      541 ctttttcggc ctcggggcga ctggctggcg gccgggcaga ctgccgtcgc aactttttaa
      601 cgtagcccag gaggacagcc aactccactc cgaaggtatc accggcttcg gagaggaaga
      661 gattacggcg ggccggatcg gccacccggc tggctaagag ggccaagtcc tggagcacct
      721 cttcttttcg tgaaaaagag agcgtcgtga aatcggcctc caggcggctc ttcttgtatt
      781 ccagaaaacc gacggcctcc tgcaggacct cctgaatcca tgactgcccg tgcttgcgtg
      841 caaacgtatc cgggtcttca ccggccggca acagagccac ccgcaccgaa aacccctgat
      901 caatcaaatg ctcaatcgag cgcaggacgg ccgactgccc ggcggcgtcg ggatcgaaaa
      961 agagaatggc ttccgacgca aagcgggcca aaaggcgcgc ctgatcggga gtgaacgccg
     1021 ttcccaaagg cgccaccaca ttcttaatcc cgacttgata caccgagatt aaatcaaggt
     1081 aaccctcgac caccaccgcc tttccggtgg aacgaatttc ctcttttgag agattcaatc
     1141 cgaaaagtgt cttccccttc tggtagagtg ggtgttccgg agagttaagg tatttcggtt
     1201 gatcttcggt attgatcacc cggccgccga aagcgatcgg cgcgttcgaa aggttgaaaa
     1261 tcggaaagac cacccgccgc cggaagaggt cgtagtaccg gctgccgccg gaataggccg
     1321 ttgcgtcctt tctggcaacc aggccgactt tgaaaagcat ttcggcgggc acaccacgtt
     1381 ccgcggcaaa ggcgatcaga ccatcccacg caaagggcgc atatcccact tgaaattctt
     1441 cgatagtttc ggcggatagt tcacgctcac ggagataggc ttttccttct ttgcccaaac
     1501 gaggatctgc aaaactttcc cggaagtaag ccagagccac ctgcgtggcg cggtagaggt
     1561 cttcactttc cttctgccct tgcggtgatt tggtcctggg cagttcgata ttagcccggc
     1621 gagccaaata ccggagcgcc tcgccgaagg tcatcttttc gtgctccgtc aggaaggtga
     1681 agacgttccc ccccttgccg cagccgaagc agtggtagac ctgcttctgc tggctgaccg
     1741 aaaacgaggg cgtcttctcc gagtggaacg ggcacaaggc gaggtaacta ttccccctct
     1801 tcttcaaggt gacgtactgc ccgattacct cgacgatatc ggaggcgtcg cggacgcggt
     1861 cgatggtgtc tgatctgtcg aactgatttt gggaagacat tgacttcccc ggttacagtt
     1921 ttccttctct tttcatttgc accaccgtca ctccctctcc cccctcgttc cactgcccca
     1981 gccgggtggc ggctaccagc ggatgattct tcaagtatgc acccacttct ttgcgcaact
     2041 tcccggtccc tttcccgtga atgataacca cttcggagag tccggacaag gcaacgccgt
     2101 cgaggtactt gtcgatctcg gccagcgctt cgtcaacggt catcccgcgc acggagagct
     2161 cccgcgagac acctttttct tcttccaatt gccggcgggg gggcgttgac gatgacggaa
     2221 ctgccgacgg ttcgactttc ttcacgatct gctcccggta gaccgtgtag aagatgttgc
     2281 cgacccacag ccgtactttt cgctcgtcgt tggccagttc cacaacttcg ccggaaatct
     2341 gctgggcacc atccgtagac agggcaaccg acacggtgtc gccgatgcgc aattcggtca
     2401 tttctggagt cacaaccggt gccaactctt ttcccttcgc cgccagttct tctgcggtcc
     2461 gttgaagcga cgtttgcgtt tctttcacca cttttttgtc cgcctgcgag cggcggatct
     2521 ccgctaccag acgttccgtc tccttgcggg tcgtacgtac aatttcctgc gcttcctcaa
     2581 gtgccgccgc tttggcctgc ctttcgcgct catccaattt ctttttcttt tccgcaatga
     2641 accgctccag ttctcccaca gtcgctttct ggagcgccac ctgtttcctg tcttccgaga
     2701 gcgcgtggag attttcttcc acttcttcca ggagtttgac cagactccgc tcctggctcc
     2761 ccaccagttc tgcggctttg ttcgtgacct ttttgggcat ccccaaccgc tcggcaattt
     2821 cgagggcgta actggctccc ggcaggccga gcgagaaacg gtacgttggc cgcaacgttt
     2881 tccgatcgaa ctctaaagaa gcgttgcgga tctgcgggta ctgctgggcc agagttttca
     2941 aagctgaata gtgggtcgtg gccacgatct tggctttttt ctccagcaaa tcgaggagca
     3001 ctgcctcacc cagggccgca ccctctttcg ggtcggttcc gacgcccagt tcgtcgaaga
     3061 ggacgagcgt agcggcatcg gcccgcgaaa gcgcctggat gatccggctc atatgagagg
     3121 agaaggtgga gagtgattgt tcaatcgact gctcgtcgcc gatgtcggcc cagacgctgc
     3181 cgaatagacc gatttccgaa tcggggctgg cgggaatgtg caggccggcc tgcgccatga
     3241 gcacgagaat ccccaaggtc ttcagggcca cggtttttcc ccccgtgttc gggccggtga
     3301 cgatcacgca ggaaaaatcc cgccccagag agaaatcgag cggtatgacc tgctttccgg
     3361 gttgcgtttt cacctgcaaa agcaaaagcg gatgccgggc gttcaggaga gaaacaaagg
     3421 ccccggcggt gatcttcggc gccgagccgt tcaaatcgac tgacaggcgg gccttggccc
     3481 ggaggaaatc caattgcgcc agaatttcca gattttcggc caattcgtcc tggtgcttgc
     3541 ggacgtgatc ggagagcgcc cgcaggatgc ggtcgatctc ctggcgctct ttgatttctg
     3601 tctcgcgcag ccggttattc agttccaccg tctccagcgg ctcgacaaaa aaagtcgccc
     3661 cggaggccga gcggtcgtgc acgataccct tttgacgcga gaggtcgtgt tcgtctaccg
     3721 ggatgacgta gcggttgtca cgcagggtga cctgtgcctg tggcgttttg cgccgggaca
     3781 gaatggcttc aagtttggct aatatctcac gccggatttt ctgcttctgc tgacgcaggg
     3841 tgtgcagagc gggcgaggcg gaatctctaa tttcgcctga ggggtcaatg gctttttcga
     3901 ttgccttttt caattcggaa tgggcagtga gcccggcgag atggctcgtt accaaaggat
     3961 aagcgttaag caaggccttt gcagtgcgga ccaggtcacc cacgagaccc gaaaaatcgg
     4021 ccacctccag gagccgcaac ggctccagaa acgttccctc cattttcacc gtagccagat
     4081 gctcccgaat gtccggatac gatcgcaggg ggaaatgctg gtcggtggta atgatttctt
     4141 ttacctcggc cgtttcggac agccacgtcg cgattcttcc gggatcggtc tggggcgtga
     4201 gcgctcccgc cagttcgcgt cccggagccg ccaaacagcg ttcgccgacc aaggcgacga
     4261 ttttatcgaa ttcgagaacc tcaagggagt gccgatccat acacctcttt atgatctatc
     4321 gcctgtgctt agagtaaccg gtaagtaata taagccgccg gttgcaaaac cgtacctcct
     4381 tctggctgca gcggtccgga atgcttccct tcggtttatg ataagcgtca tagcacgaat
     4441 ttcgcccgat ccatattgtt gcgaaacgtt atcggtaagt gcccggcccc ggaagtggcc
     4501 cgggcgagag gcgtcttaaa ggaggttctg atggaacgga tgcgaccggg tgtgttggta
     4561 ctcacgttca tgttcctttt ctcttcactt tttctgctct ccagtgtccc cgcccggggg
     4621 gaaaacgctg cactgccggc aaagggagac gaaagtaatt ccccggacag cgcccgaatg
     4681 ccggtctacc agttagacgg catcgtcgtc accgccaacc ggtacgagaa atcggctttt
     4741 gccgtcccct acgcgatgtc gatcctctcg gctgataaat ttcagcaggc cggggcgccg
     4801 atcatcgccg acctcttgcg caacctggcc ggtgtcgatg taaccgatgc cggaccgttc
     4861 cgcacccggc ccatcatccg ggggctggcg ggcagccgca tcctggtgct ggtggacggt
     4921 cagcgcatga atgacacccg cgagaacact ttttccggcg cggaactgtc gcaggtgagc
     4981 ccgggtcagg tcgagcgggt ggaggttttg cgcgggtcga actcggtcct ctacggctcc
     5041 aacgccatgg gcgggatcat caatattctc acccggcagc cgtcggcgcg gcgggggccg
     5101 cttgcggtgt ccgggagtct ggtcaaccgg tacagcacca ccgaccagca gccctactcc
     5161 cggctgaatc tcaacctcgc caaccaccgc tggcgcttgg cgggcggcgc cgagtaccgc
     5221 cgcgccaata actaccatgc accgggccga agcgttttcg gccggggccg cgaaattcaa
     5281 aattcaaatc tgcgccgctc ggtggggctg gattttgccg gcagttaccg gctggcggac
     5341 cggcacacgc tcgcgctgga agcgcagagc atgctgaact cggagatcgg tttcccggaa
     5401 acgcccaatc cgacttttcc ggtgggtatc ttcttcccct atcatgaccg gtcaaaaatc
     5461 gccctgacgt acgaagggaa gaagattaca ccgcgtctgg tctccatccg tgccacgctc
     5521 tacggccaga ag
//
LOCUS       DBNGZA010000156         1342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_982553, whole genome shotgun sequence.
ACCESSION   DBNGZA010000156 DBNGZA010000000
VERSION     DBNGZA010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_982553"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16FU_03340"
     CDS             <1..335
                     /locus_tag="AC16FU_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR2993442.1"
                     /translation="SQGIKAKCQQLLFVSGQIASKPENGELVAGGIIEQTKQALENVK
                     AIVAAGGGTLADVVKTTVYLTNFDDFTLMNETYSRFFAEMPPARATVQVAKLPKGAKI
                     EIDVVAAL"
     gene            390..>1342
                     /locus_tag="AC16FU_03345"
     CDS             390..>1342
                     /locus_tag="AC16FU_03345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2993443.1"
                     /translation="MDFQLTEDHLAIQQLVRDFAAKEMAPGLRERDRQEKFDPAILKK
                     LGAADILGLCIPAKYGGAGFDYISLGLACEELEYVDTAGRVILSVHIGLNSLALLTWG
                     TEAQKQKYLAPQAKGDKIATFGLTEPNAGSDAVGIQTTADREGDAFVLNGEKMWISLA
                     DVADNFLIFAWTDREKKKKRDHTGLSAFIVERSLGVKTGTIHGKLGVRAGNTGFVSLA
                     DVRVPKENMLGQEGEGFKIAMFCLEQGRFTVAAGSTGLIKACLDACVAYAKTRKTFGE
                     SIAQHQLVKEMIAKMVAGYETSRLLWLRAAWLKNQGLRNTR"
BASE COUNT          303 a          368 c          426 g          245 t
ORIGIN      
        1 acagccaggg gatcaaagcg aagtgtcagc agctgctttt cgtctccggt caaatcgcct
       61 caaaaccgga gaacggtgaa ctggtggcgg gtggcatcat tgagcagacc aagcaggcac
      121 tggagaatgt caaagcgatc gtcgcagccg gcgggggcac gctggcagac gtggtgaaga
      181 ccacggtcta cctgacgaac ttcgacgatt tcaccctgat gaatgaaacc tacagccggt
      241 tttttgccga aatgccgccg gcccgggcca ccgtgcaggt ggccaagctc cccaaaggcg
      301 cgaaaattga aatcgacgtc gtcgccgcgc tgtaaatttc cagcggagcc ggaacgggaa
      361 acgatttctc ctgtattgag ggagatatga tcgactttca acttaccgaa gaccacctgg
      421 ccattcaaca attagtccgc gatttcgctg cgaaggaaat ggcgcccggc ctgcgggagc
      481 gcgaccggca ggagaagttc gacccggcca tcctcaagaa attgggtgcg gccgacatcc
      541 tggggctctg tatcccggcc aagtacggcg gggcgggatt tgactacatc agtctcggcc
      601 tggcctgcga ggaactggag tacgtcgaca ccgccgggcg ggtgatcctc tcggtgcata
      661 ttggactcaa tagtcttgcg cttttgacct ggggcaccga agcgcagaag cagaaatacc
      721 tcgccccgca ggcgaaaggt gataagattg caaccttcgg cttgaccgaa ccgaacgccg
      781 ggtcggatgc ggtggggatt caaaccaccg ccgaccggga gggcgatgct ttcgttctga
      841 acggcgagaa gatgtggatt tctttagccg acgtggccga caactttttg attttcgcct
      901 ggaccgaccg ggagaagaaa aagaagcgcg accataccgg gctctcggcc ttcatcgtgg
      961 aacgcagctt gggggtcaaa accggcacca tccacggcaa gttaggcgtg cgggccggga
     1021 acaccggttt cgtttcactg gccgatgtgc gcgtaccaaa ggagaacatg ctgggccaag
     1081 agggcgaggg attcaagatt gcgatgttct gcctggaaca ggggcgcttc acggtggcgg
     1141 ccggctccac ggggctgatc aaggcctgcc tggatgcctg cgtcgcctac gccaaaacgc
     1201 gcaaaacctt cggagagtcg attgcccagc accagttggt gaaagaaatg atagccaaga
     1261 tggtggcggg gtatgagact tcgcgcctct tgtggctgcg tgccgcctgg ctcaagaacc
     1321 agggactgcg caacacgcgg ga
//
LOCUS       DBNGZA010000157         3124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_453267, whole genome shotgun sequence.
ACCESSION   DBNGZA010000157 DBNGZA010000000
VERSION     DBNGZA010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_453267"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1164
                     /locus_tag="AC16FU_03350"
     CDS             133..1164
                     /locus_tag="AC16FU_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993444.1"
                     /translation="MEKKKAHAIALFSGGLDSSLAILLMRQQGIEVTALSFLTHFGCD
                     LSDRSSCGHNPYPVAEKFGFTVKLVHLGMEYVEAVKKPRYGHGKNMNICIDCRLIMLS
                     KAKEYMELTGADFVITGEVLGQRPMSQRWETLRMTDKQSGLQGRLLRPLSAKLLPPTV
                     PELEGLVDREKLLDIEGRSRHRQMELAKELGLEDYPSPASGCLLTDPGYSQRLRDLLA
                     HKTDADFTDINLLRVGRHFRLSPTCKVIVGRNDTENQIIKGLAREGEWLLEVPGTGSP
                     AVLFQIVGTNGNGSPDPEWLLTAARLTARYCDLKNQPEVEVTVTQNGSVESLTVSPSP
                     DDLVNSLRI"
     gene            1208..1282
                     /locus_tag="AC16FU_03355"
     tRNA            1208..1282
                     /locus_tag="AC16FU_03355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Arg,seq:cct)
     gene            complement(1633..2292)
                     /gene="phoU"
                     /locus_tag="AC16FU_03360"
     CDS             complement(1633..2292)
                     /gene="phoU"
                     /locus_tag="AC16FU_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR2993445.1"
                     /translation="MERHFDEELNEIRKKLVAMAGLTEQLISRSIQVLVERDKSVAEE
                     VWPGEDSVNKKQIEIDEDCLRLLALHQPMAVDLRFLVATLRITGELERIADQAVNITQ
                     ASLVLIVRPEVKPLIDIPKMAALAAGMVKDSLDAFVKKDAGLAHAVLSRDDEVDAYKD
                     QIFRELLTYMLSDPKTIERGIELILISRHIERIADHATNIAEDAIYYVEGRDVRHHAG
                     E"
     gene            complement(2292..3035)
                     /gene="pstB"
                     /locus_tag="AC16FU_03365"
     CDS             complement(2292..3035)
                     /gene="pstB"
                     /locus_tag="AC16FU_03365"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR2993446.1"
                     /translation="MVVERFSFWYTKTKALNAVSTKVYQHQITAYIGPSGCGKSTFLR
                     ALNRMNDLIPYTRWEGKILLDGVDITRPQLDVTELRRRVGMVFQKSNPFPKSIFENIA
                     FGLRIAGEISPRKLSVAVEEGLRQAALWDEVKDKLGRSALALSGGQQQRLCIARALAV
                     KPEVLLLDEPCSQLDPISTAKIEELLLNLKQHYTIVLVTHNMQQAARISDYTGFFLLG
                     ELIEFDATPKLFRTPADKRTEDYITGRFG"
BASE COUNT          660 a          900 c          828 g          736 t
ORIGIN      
        1 ccttgtggtt tcctccgtgt actccgtgcc tccgtggtga gatttgcctt gcctttcacc
       61 aaatcccaac tcctaaatcc caccttttcc ccgattgacc accctcaccc catctggtat
      121 cttaaaattg ctatggaaaa aaagaaggcc cacgccatcg cccttttctc cggcgggctc
      181 gatagttcgc ttgccatcct ccttatgcgc cagcagggga tcgaggtaac cgccctgtcc
      241 tttttgaccc acttcggctg cgacctctcc gaccgctcct cctgcgggca taatccctac
      301 ccggtggcgg agaaattcgg cttcaccgtc aagttggtgc acctggggat ggagtacgtg
      361 gaggcggtca aaaagccgcg ctacggccac ggcaagaata tgaatatctg catcgactgc
      421 cggctcatca tgctcagcaa agcgaaagag tatatggaat tgaccggcgc cgatttcgtc
      481 atcacgggcg aagtgttggg ccaaaggccg atgtcccagc gctgggagac tctgcggatg
      541 accgacaaac aaagcggatt acaggggcgt ttgctccgac ccctttcggc caaacttttg
      601 ccacccaccg ttccggagtt ggaggggctg gtggaccgcg aaaagttact ggatattgag
      661 ggccgctccc gccaccgcca aatggaactg gctaaggaac tggggctgga ggactaccct
      721 tctcccgcat cggggtgtct cttgaccgat cccggctact cccagcgcct ccgcgatctg
      781 ttggcgcata agaccgacgc cgatttcacc gatatcaatc tcctgcgcgt gggccgccac
      841 ttccggttgt ctccgacctg caaggtaatc gtgggccgga acgacacgga aaaccagatc
      901 atcaaaggtc tggcgcgaga gggcgaatgg cttctggagg tgccgggcac cggcagccct
      961 gcggtgctgt ttcagatagt cgggacaaat ggcaacggct cacccgatcc ggagtggctt
     1021 ttgaccgcag cacgtttgac cgcccgctac tgcgatctga agaaccaacc ggaagtggaa
     1081 gtgacggtga cccagaacgg ttcagtggag agcttgactg tctccccctc tcccgatgat
     1141 ttggtcaact ctttacgcat ttgactttca tcacccatca ctctgtcact aatcactgtt
     1201 cttatccgcc cccgtagctc aggatggata gagcagcggt ttcctaaacc gttggccggc
     1261 cgttcgaatc ggcccggggg tactttttca aaaccgtttg cgcgtcaagc ggttcaagcc
     1321 ggtcgactgc cgcgcatttg acgtcggtgt ttgaccctgc tacgactttt gctacgacat
     1381 ttgctacagc tcgaggtagt tattggaaca ttaaaagcgt tctactcgca tatcacgctt
     1441 cactcaatta tttagaaaat ttaacgtagc acgctggcat ctaacaggtt gctacgaaaa
     1501 catcgattgg actaaaacag ttcttagaac ggagcagagg tctcaaaaac cgctgtccaa
     1561 cgtattttct tatacgtggc agttctgaat ctgtaaatcg ttcgaaataa tcggtgcact
     1621 tttcgcctta gattattcgc cggcgtggtg gcggacgtcg cgcccttcca cgtagtagat
     1681 ggcgtcttcg gcgatgttcg tggcgtgatc ggcaatccgc tcgatgtggc gggagatcaa
     1741 gatcagctca atgccccgct caatggtctt cgggtctgag agcatgtagg tcaacagttc
     1801 ccggaaaatc tgatctttat acgcatccac ctcgtcgtcg cgggaaagca ccgcatgggc
     1861 gagcccagcg tccttcttga cgaaggcgtc cagactgtcc ttgaccatac cggcagccag
     1921 cgcggccatc ttggggatgt cgatgagagg tttcacttcc ggtcggacaa ttaaaaccag
     1981 cgacgcctgg gtgatgttca ctgcctgatc ggcaatgcgc tccagttcac ccgtaatgcg
     2041 taacgtcgca actaaaaatc gcaaatcgac tgccatcggt tgatgcaaag ccagcaagcg
     2101 caagcagtct tcatcgatct caatctgctt tttgttgacc gagtcttcgc ctggccagac
     2161 ctcttcggcc acggatttat ctcgctccac caagacctgg atcgagcggc taatcaactg
     2221 ttccgtcagg ccggccatgg cgactaactt tttccggatt tcattcaact cttcatcgaa
     2281 gtggcgttcc attatccgaa cctcccggtg atatagtcct cggttctctt gtccgctggc
     2341 gtccggaaga gcttgggtgt ggcgtcgaac tcgatcagtt cacccaagag gaaaaagccg
     2401 gtgtagtcgg aaatgcgggc cgcctgctgc atgttatggg tgaccaggac aatcgtatag
     2461 tgctgtttga ggttgagcaa tagctcctca attttggcgg ttgaaattgg gtccaattgg
     2521 gagcagggtt cgtccaggag gaggacctcc ggcttgaccg ccagcgcccg ggcgatgcag
     2581 agccgctgct gctggcctcc ggaaagagcg agcgccgacc ggcccaactt gtcctttacc
     2641 tcatcccaca gagcagcctg ccgcaaaccc tcttctaccg caacggaaag tttccggggg
     2701 ctgatttccc ctgcaatccg caggccgaag gcaatgttct caaaaattga tttggggaac
     2761 gggttcgatt tctgaaaaac catgccgact ctgcgccgta attcagttac gtcgagctgg
     2821 ggacgtgtga tgtcaacccc atccaagagt atcttgcctt cccaccgggt gtacggtatc
     2881 aaatcattca tccggtttaa ggcccgcaga aacgtcgact tgccgcagcc ggaagggccg
     2941 atgtacgcag ttatttggtg ctggtaaacc ttcgtgctga ccgcattgag ggcctttgtt
     3001 ttcgtgtacc agaaggaaaa gcgttcgacg acgattttct cctgctcagc cgcatcggcc
     3061 gcattccgtc tggtgggctc aagctttaac cgcggaccct caggcaccgt ttcccattcc
     3121 acgt
//
LOCUS       DBNGZA010000158         2319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_771278, whole genome shotgun sequence.
ACCESSION   DBNGZA010000158 DBNGZA010000000
VERSION     DBNGZA010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_771278"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16FU_03370"
     CDS             <1..198
                     /locus_tag="AC16FU_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993447.1"
                     /translation="QLGALLAEMLREPDKRAHGLHRVMHRVKDYDLQRRLEKIEADLA
                     DIRHTFAVAKRLTEQCFGERA"
     gene            209..403
                     /locus_tag="AC16FU_03375"
     CDS             209..403
                     /locus_tag="AC16FU_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993448.1"
                     /translation="MMKDDSVARQLEALLNESDKCAHAIQKLIPKIGDYDLVRLLKKI
                     DADLADIRHNLAVTKRLAEK"
     gene            518..>2319
                     /locus_tag="AC16FU_03380"
     CDS             518..>2319
                     /locus_tag="AC16FU_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2993449.1"
                     /translation="MIGQTISHYKISAKLGEGGMGVVYKAQDLRLDRTVAIKFLPEHL
                     TADPAARTRFVAEAKGASAINHQNITTVYDIDEADGKSFIVMEYLEGKSLKDLAQEGV
                     RINRVSDIGIQIAEGLQAAHRKEIVHRDIKSENILVTKDSVVKIMDFGLAKLKGPSHL
                     TQTGATLGTVAYMSPEQTRGEEVDHRSDLFSLGVVLYELLTGKLPFSGEHQAAIIYSI
                     CHEAPEPLARYKAGIPEELQRVVTKTLQKDRNLRYQSAADLGADLKALRKVEEGTGAP
                     VSPPEPVRRQKTWVAVAIALIFVVIAGYLLFSRFFGPKGEKSEARRKMLAVLPFENLG
                     SPEEEYFATGITEEITTRLAKIGGLGVISHTSARKYKKTEKTLKQIGAELGADYILEG
                     NIRWAKSDGASRIRINPQLIQVSDDSHLWAENYDAVLNDVFELQSSIAEKVAAALDVA
                     LLEPERRVLAAKPTENLEAYDFYLRGNDQFNRTSMVGVGSEKYLRIAFQMYERALDLD
                     SNFALAHCQFSRVNTELYWHYDRKDQYLKNANEGVEKAFKLSSGLPEAYLALGSLHYH
                     AGEYEKALEEFAIAQKGLPNNPDLLKEIGFVQRR"
BASE COUNT          593 a          596 c          650 g          480 t
ORIGIN      
        1 caactcggcg cgttgttggc tgaaatgctc agggaaccgg ataagcgcgc ccacggcctt
       61 caccgagtaa tgcacagggt gaaagactac gacctccagc ggcgtttgga gaagattgaa
      121 gccgatctcg ctgacatccg gcataccttt gcggtagcca agcggctgac ggaacagtgc
      181 ttcggggaaa gggcgtaagc gggctatcat gatgaaagat gactcggtgg cccggcaact
      241 cgaggccctc ctcaatgaat cggacaagtg tgcgcacgca atccagaagt taattcctaa
      301 gatcggcgac tatgacctgg tgcgcctctt aaagaaaatt gatgccgacc tggccgacat
      361 ccgccacaac ctggccgtga ccaaacgcct ggcggaaaag tagtctccca ccggtatggg
      421 tgaagccact gctggtctgc tcttcccaca ggtggttttg agtcccccgg cccccgccgg
      481 tgctactcct gaatcgtaaa atcaagacaa cgcaattatg atcggccaga cgatctctca
      541 ctacaaaatc tcagccaaac tcggtgaagg gggaatgggc gtcgtctaca aggcccagga
      601 cctccggtta gaccgcacag tggccattaa gttcttgccg gagcacctca ccgctgatcc
      661 tgccgccaga actcgattcg tcgccgaagc caaaggcgcc tcggccatta accaccagaa
      721 tatcaccacg gtgtatgaca tcgacgaagc ggacggcaaa tccttcatag tcatggagta
      781 cctcgagggg aaatccttga aagacctggc gcaagaagga gtaagaataa atcgggtttc
      841 ggatattggg attcagatcg ctgaggggtt gcaggccgcc cacagaaaag agatagtgca
      901 ccgggacatc aagtccgaga acattctggt gaccaaagac agcgtggtga agattatgga
      961 cttcggcttg gccaagctga aagggccgtc ccatctcacg caaaccggtg ccaccctcgg
     1021 cacggtggcc tacatgtccc cggagcagac gcggggggag gaagtagacc accgaagtga
     1081 tctgttttct ttaggcgtag ttttgtatga gttactaacg ggcaagttgc ctttttcggg
     1141 cgaacaccag gcggccatca tctattcaat ctgtcatgaa gcgccggaac cgttagcgcg
     1201 ctataaagct ggcattcccg aagaactgca gcgggtcgtg actaaaacgc ttcagaagga
     1261 ccgcaatctt agataccaga gtgcggctga cctgggagcg gacctgaagg cgctgagaaa
     1321 ggtggaggag ggcaccggcg ctccggtatc acctccagag ccagtccgaa ggcaaaaaac
     1381 ttgggtcgct gtggcgattg ctttaatctt tgtcgttatt gcgggatatt tgctattctc
     1441 gcgctttttt ggcccaaaag gagaaaaatc ggaagctcga agaaaaatgc tggcagtttt
     1501 gccgttcgag aatttggggt cgccggaaga ggagtacttt gcgactggca taacggagga
     1561 gattaccacc cgtttggcga aaatcggcgg gttgggcgtc atttctcaca ccagtgcccg
     1621 taaatacaaa aagaccgaaa aaactttgaa gcaaatcggc gccgaactgg gggcggatta
     1681 cattctggaa ggaaatattc gctgggctaa atcggacggc gccagccgca ttcgtataaa
     1741 tccgcagctg atacaggttt ccgacgactc acacctttgg gccgaaaact acgacgcggt
     1801 attaaacgac gttttcgaac tgcagtccag catcgccgag aaagtggcgg ccgctttgga
     1861 tgtggccctg ttagagccag agcggcgcgt gctggccgcc aaaccgacag aaaatttaga
     1921 ggcctacgac ttttacctgc gcggcaatga ccagttcaat cgcacttcca tggtaggggt
     1981 cggatctgaa aaatatttga gaatcgcctt tcaaatgtat gagcgggcgc tcgatttgga
     2041 ctccaatttt gccttggccc attgccagtt ttcaagggtc aataccgagc tgtactggca
     2101 ttacgacaga aaggaccagt atcttaaaaa tgccaatgaa ggggtggaaa aggcctttaa
     2161 gctttcttcc ggcctgccgg aagcctattt ggcgcttggc tccttacact accacgcagg
     2221 ggagtacgaa aaagcgctcg aagagttcgc cattgcccaa aaggggctgc ccaacaatcc
     2281 cgacctttta aaggagattg gatttgtgca aaggcgtaa
//
LOCUS       DBNGZA010000159         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2679375, whole genome shotgun sequence.
ACCESSION   DBNGZA010000159 DBNGZA010000000
VERSION     DBNGZA010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2679375"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="AC16FU_03385"
     CDS             <1..1047
                     /locus_tag="AC16FU_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993450.1"
                     /translation="SKHKPGIVIGNRSEEERNRKGITYDDALAILERCPAVAAVSPEN
                     HYYPFPSGNRVKYKNQSARRPDFLGVLPAYEQVNNSFVSEGRFITESDHQYRSMVVVV
                     GQDVADALFGRLDPIGKEILVNNDKFLVIGVVEKRESILGQSQNNFVLIPYGTFAKIH
                     PEEKELWLAVQAKSRADMPAATDQIRDLMRRRRGVPYDKPDNFAVFTQDDLLQVYNQV
                     TFAIYMVMIVISSIGLLVGGVGVMNIMLVSVTERTREIGIRKAIGAKRNNILYQFLIE
                     AMTLSGTGGILGILFGVLVSQFVAAVSPLPAAVSGFWVFLGFTVAVSVGLIFGIYPAY
                     RAAKVDPIVSLRYE"
     gene            1141..>2452
                     /locus_tag="AC16FU_03390"
     CDS             1141..>2452
                     /locus_tag="AC16FU_03390"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2993451.1"
                     /translation="MVRFCRLTSFPLLLTVVLFTLTVAPAKAQYGFQSREPLAGQPKF
                     QLDAAAFGLFDEGKVRVEVYYRIPADGLRFAKEKDRYRAEYEMAVVLADKKGRQIDAT
                     TFSEKISVDSYQETQSKGDLLINQLNFTALPGEYKVVATLTDRNSGQTYSQTDRLKAQ
                     PFQGGAVTLSTLEFALTVIDSTEAGRFQKGHRFVLPAVSRLYSDSLPRLVLYYEVYSR
                     EKQPSTFQVDYFAASRSLRKRVIDTATVTAVPGVTPVVASLNVSRLPPGAYTLSAVVK
                     VSKGKEVCHQEGEFIIAWSLASILRNDFFGAVEQLRYIATAAEMKALKRSRSAERLKN
                     WDEFWRRKDPSPGTPENEAAEEYFRRIRYANANFTVGLREGWKTDLGRVYVIYGEPDQ
                     IEVHPFERDAKPYQVWFYYQSTRSLFPRTFVFVDETGFGDYELQY"
BASE COUNT          492 a          750 c          726 g          484 t
ORIGIN      
        1 tccaagcaca aaccggggat cgtcatcggc aaccggtccg aagaggagcg caaccggaag
       61 gggatcacct acgacgacgc cttggccatt ctggagcgct gcccggcggt ggcggcggtc
      121 tcgccggaaa accactacta cccgtttccc tccggcaacc gggtcaagta caaaaaccag
      181 agcgcccggc ggcccgattt tttgggcgtc ctgccggcgt acgaacaggt gaacaattcg
      241 ttcgtttccg aggggcgctt cattaccgag agtgaccacc agtaccgctc gatggtcgtc
      301 gtggtgggcc aggacgtggc cgacgccctc ttcggccggc tcgaccccat cggcaaagag
      361 attctggtca acaacgacaa gtttctggtg atcggggtag tggagaaacg cgagagcatt
      421 ttgggccaga gccagaacaa cttcgtcttg attccctacg gcaccttcgc caaaatccat
      481 ccggaggaaa aagaactctg gctggcggtg caggccaaaa gccgcgccga catgccggcc
      541 gccaccgatc agatccgcga cctgatgcgc cgccgccggg gcgttcccta cgacaaaccg
      601 gacaacttcg ccgttttcac ccaggacgac ctgctccagg tctacaatca ggtcaccttc
      661 gccatctata tggtcatgat cgtcatctcc tccatcggcc ttttggtggg cggcgtgggg
      721 gtgatgaaca tcatgctggt ctcagtcacc gaaaggaccc gcgagatcgg catccgcaag
      781 gcgatcggcg ccaaacggaa caacatcctc taccagtttt tgattgaagc gatgaccctc
      841 tccggcaccg gtgggattct gggcatcctc ttcggcgtcc tggtgagcca gttcgtggcg
      901 gcggtgtcgc ctctgccggc cgcagtttcc ggtttctggg tttttctggg gttcaccgtg
      961 gcggtttcgg tcgggctgat cttcggcatc tacccggcct accgggcggc caaggtcgac
     1021 ccgatcgtgt cgctgcgcta cgagtagttt tttctgcccg aaagagtgcc cgcagccgta
     1081 tcaacaaagg caatggggaa tcttttgctc ttttccggtc aaagcgtatc ttttcccgtg
     1141 atggtgaggt tctgccgatt gacctctttc cctttgcttt tgaccgtggt cctgttcacc
     1201 ctgaccgtgg caccggccaa agcgcagtat ggctttcagt ccagggaacc tctggcgggc
     1261 cagccgaagt tccaacttga tgcggcagct tttgggctct tcgatgaagg caaggtgcgc
     1321 gtggaggtgt attaccggat ccccgcggac gggctgcgtt ttgccaaaga aaaggaccgc
     1381 taccgggcgg aatacgaaat ggctgtggtt ctggccgaca aaaaaggcag gcaaatcgac
     1441 gccaccactt tttcggaaaa aatctccgtc gattcgtacc aggaaacgca gtccaaaggc
     1501 gacctgttaa tcaaccagtt gaacttcacc gccctccccg gcgaatacaa ggtggtggcg
     1561 actctgaccg accgcaattc gggacagacc tattcgcaga ccgaccggct caaagcgcag
     1621 ccattccagg gtggtgcggt gaccttaagc actctggaat tcgccctcac ggttatcgac
     1681 tcaaccgaag cgggacgatt ccagaagggc caccgcttcg tcctgccggc ggtgagccgg
     1741 ctctacagcg atagcctgcc gcgcctggtg ctctactatg aagtttacag ccgggagaaa
     1801 cagccgtcga cgtttcaggt cgactatttc gccgccagcc gaagtctgcg gaagcgggtc
     1861 atcgacaccg cgaccgttac cgcagtgccc ggggtgacgc ccgtcgtcgc ctcgctgaac
     1921 gtcagccgcc tgccgccggg ggcctatacc ctgtcggccg tggtgaaagt gtccaaaggg
     1981 aaagaagtct gtcaccagga gggtgagttc atcatcgcct ggtctctggc cagcatcctg
     2041 cggaacgact tcttcggcgc ggtggagcaa ctgcgctaca ttgccacggc ggcggaaatg
     2101 aaggcgctca agcggtcgcg ctccgccgaa cggctgaaga actgggacga gttctggcgc
     2161 aggaaagacc cgagtcccgg cacgccggaa aacgaagcgg cggaggaata tttccgccgc
     2221 atccgctacg ccaacgccaa cttcaccgtg ggcttgcggg aggggtggaa gaccgactta
     2281 ggccgcgtct acgtcatcta cggcgagccg gatcagatcg aagtgcaccc gttcgaacgc
     2341 gacgccaagc cgtaccaggt ctggttctac taccagagca cccggagtct cttcccgcgc
     2401 accttcgtct ttgtggatga gaccggtttc ggcgactacg aactccagta cc
//
LOCUS       DBNGZA010000160         2308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1513148, whole genome shotgun sequence.
ACCESSION   DBNGZA010000160 DBNGZA010000000
VERSION     DBNGZA010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1513148"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="AC16FU_03395"
     CDS             complement(<1..1051)
                     /locus_tag="AC16FU_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2993452.1"
                     /translation="MTSSVRFFVRSFWGLAIFLGLLLPFSSASGQLRLIFPPFTGIAP
                     FDSLGYSVHDAGDLNGDGIVDFLIGAPRADIAGPDAGAVFVISGLTGAPLPYSPILGF
                     PPMPGANLGRSVHGLGGDVDFDGVPDFVVGAPGAFGGVGAAYVVSGATGALIPPAITG
                     FVPPGSLLGWSVSSAGMVDGDPFPDVVVGAPGFAGAGGIAFVVSGIAGHPVIFAIPNP
                     FGPGALLGYSVAGLGDIMPPFGSADVIVGAPGAFGSAGRAVLVSGPAGAPYPYSPILG
                     YAPVGPPVAAPGDSLGYSVHGLGDINFDGAPDFIIGAPGADVVPFDAGAAVAYNSLTG
                     AALTASIFSVPPVPGD"
     gene            complement(1417..>2308)
                     /locus_tag="AC16FU_03400"
     CDS             complement(1417..>2308)
                     /locus_tag="AC16FU_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993453.1"
                     /translation="ISFGSVVQTSGTAYPWLYHQQYLNEHQTYFSSALIPHLIKLGFY
                     SFAQNAFHYGNWILTAIVAGFLIFRLSNWQQKCRPLVWTLAAACVFMGVHTFIRWSVR
                     PWYPQAVFVLTLPLVALALERMNRFWLGLCIVAVLFFSGWAVWSTPLWLANRSKVMLE
                     IVNGQIPAEDRVGIFNSGYLQYFTDRKVINLDGLVNNEVLPYYRKHKGIEYLRKRNIA
                     WLVDRSIYLGAVFGPYLGPAAESTLSLVKVWQDSLHPEIAVGIVQVLPDSLRPLPERR
                     MRRLKEPTHYQWGPLPFLKP"
BASE COUNT          605 a          668 c          643 g          392 t
ORIGIN      
        1 gatcacccgg gacaggggga acggaaaata tcgaggcggt aagcgccgca ccggtgagac
       61 tgttgtaggc caccgccgcg ccggcatcga acggcaccac gtcggccccc ggagcgccga
      121 tgatgaaatc aggcgcgccg tcaaaattga tatcccccag cccgtgcacc gaatagccca
      181 gcgagtcgcc cggagcggca acaggcggtc caaccggggc gtagcccaga ataggcgaat
      241 agggataggg agcacccgcg gggccggaaa cgaggacggc cctcccggca ctgccaaagg
      301 caccgggtgc gccgacaatg acatccgcag agccaaacgg cggcatgatg tctcccagcc
      361 cggccaccga ataacccaat agtgccccgg gaccgaatgg gttgggtatc gcaaaaatta
      421 ccggatggcc cgcaatcccg gatacgacaa aagcaatgcc gccagcgcca gcgaaaccgg
      481 gagccccaac cacgacatca ggaaacggat cgccgtctac cattccggca gatgatactg
      541 accagcccaa gaggctgccc ggggggacga aaccggttat cgccggcgga attagagcgc
      601 cggtggcccc ggaaacgacg tacgcggcgc cgacaccacc aaaagcgccg ggagcgccca
      661 cgacgaaatc gggcacacca tcaaaatcca cgtcaccccc cagcccatgt accgagcggc
      721 ccagattggc gccgggcatg ggcggaaagc cgagaatcgg tgagtagggc agcggtgcgc
      781 cggtgaggcc ggagatgaca aacaccgccc cggcatccgg gccggcgatg tcggcccggg
      841 gggcgccgat cagaaaatcg acgatgccgt cgccgttcag gtccccggcg tcatgcaccg
      901 aatagcccag agagtcgaac ggggcgatac cggtaaaagg cgggaaaatc aggcgcagtt
      961 gtccggaagc cgatgagaaa ggcaataaca gacccaagaa aatcgccaat ccccaaaagc
     1021 tccggacaaa aaaacgaaca gaactggtca tactacctcc tcggacctaa ttgttcctct
     1081 gcaaaaactg ccgcataagc atcgtgacga gacgtttcgc ccgcactttc aggcacgtat
     1141 cacctccatt aacgtatctc ccttcagtgc gagtatcccc cctcgctccg gtaacgtagc
     1201 tacccagccc ccgcctgtca agcatttttt gcgtttgaaa gaaaattttg tcaatttcca
     1261 gtagcatatc agagcaaacc tctcttatcc ttaatccgag aaacttgggg tcgaagtccc
     1321 ccgcaggcgg ggtagaaaga cgcaggtgta gctctggcat cgactgatcg acggtgttaa
     1381 gtcagggcaa caccgattgc agcagtccga ccgtggttac ggttttagga atggaagggg
     1441 gccccactgg tagtgagtgg gctctttcag gcggcgcatt ctcctttcgg gcagtggccg
     1501 caaactgtcc ggcagaacct gaacgatccc gacagctatt tcgggatgaa gactatcctg
     1561 ccaaaccttt accagagaaa gagtactttc ggctgccgga cccagatagg gtccgaaaac
     1621 ggctcctaag taaatcgatc tatccacgag ccatgcaata tttcttttac gcaaatactc
     1681 tatccctttg tgttttctgt aatacggcag gacttcattg ttcaccaatc cgtcaaggtt
     1741 aattaccttc ctgtccgtga agtactgcaa atagccggag ttgaaaattc ccactcgatc
     1801 ttccgcgggg atctgaccgt ttacgatctc cagcatgact ttggaacggt tggctaacca
     1861 taagggtgtt gaccacacag cccagccgct gaaaaagaga acagcgacga tgcaaagtcc
     1921 aagccagaaa cggttcatgc gttctagggc aagcgctacc agcggcaacg tcagtacgaa
     1981 aaccgcctgg ggataccaag gccgaaccga ccagcgtatg aaggtatgaa ctcccatgaa
     2041 aacgcaggca gctgccaagg tccacaccaa tggccggcac ttctgctgcc agttgctgag
     2101 acgaaagatg agaaaaccag ccacaatagc cgtgagtatc caattgccgt aatggaaggc
     2161 gttttgcgca aaactataaa acccgagttt tatcagatga ggaattaacg cggaagagaa
     2221 gtaggtctga tgttcattca ggtactgctg gtggtagagc caagggtatg ccgtgccgga
     2281 ggtttgcacc acagaaccga aagagatg
//
LOCUS       DBNGZA010000161         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1302387, whole genome shotgun sequence.
ACCESSION   DBNGZA010000161 DBNGZA010000000
VERSION     DBNGZA010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1302387"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1326)
                     /gene="purB"
                     /locus_tag="AC16FU_03405"
     CDS             complement(31..1326)
                     /gene="purB"
                     /locus_tag="AC16FU_03405"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR2993454.1"
                     /translation="MIARYTRPEMAKLWSEETKFKTWLQIEILACEAQAQLGVIPAEA
                     VPIIKQKARFEVAAIEKIEATVKHDVIAFLTNVALSVGKESRFIHYGLTSSDVVDTAT
                     AYLLVQAGKQIVADLQKAVHTLKRLALRYKRTRMIGRTHGMHAEPTTFGLKLLGWYDE
                     IKRQQVRMERAIEAVAVGKISGAVGNFAGVDPEVERYVCEKLGLKPSVVATQVVSRDR
                     HAEYLGTLALIAATVEKIATEIRHLQRTEVGEVEEPFAAGQKGSSALPHKKNPVTCER
                     LCGLARLVRSQAQVALENIALWHERDISHSSAERIIFPDATTLVDYLLATLDWILAHL
                     VVHPDRMRANLDQTGGLVYSGRVLLALAQNGLTREQAYGIVQSNALKSLRLKRPFREL
                     IARDKRVTRQVGAKKVQEFFDEKPYLKHVDYIFKQVLGG"
     gene            complement(1345..>1955)
                     /gene="fsa"
                     /locus_tag="AC16FU_03410"
     CDS             complement(1345..>1955)
                     /gene="fsa"
                     /locus_tag="AC16FU_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR2993455.1"
                     /translation="EKAGFEEILREICRIVDGSISAEVVSVEFDAIMREARQLAKLHK
                     NIVVKIPLIKEGLKAIKACSAEGIRTNVTLVFSANQALLAAKCGASIISPFVGRLDDV
                     GHDGMEVVQQIIEIYDNYDYPTEVLVGSVRHPLHVTRAAQMGADIATCPFKVLDQLMK
                     HPLTDVGLKNFLADWEKARKALPEAAAVKKEKARVPTAVGRG"
BASE COUNT          366 a          602 c          557 g          430 t
ORIGIN      
        1 agagccgtca ggacaaacgg ccatcccgct ttagccaccg agcacctgtt tgaaaatgta
       61 atccacgtgt ttcaaatacg gcttttcgtc gaaaaactct tgcacttttt tcgcgccgac
      121 ctgccgggtc acccgtttat cccgggcgat taattcccgg aagggacgct tcagccggag
      181 ggacttgagc gcgttcgact ggacgattcc atacgcctgc tcgcgggtaa gcccgttctg
      241 cgccaaagcc aagagcaccc gccctgagta aaccagcccg ccggtttggt ctaagttcgc
      301 gcgcatccgg tcgggatgaa cgaccaggtg cgcgagaatc caatcgagcg tcgctaaaag
      361 gtaatcgacc agcgtggtcg cgtcgggaaa gatgattcgt tccgccgaag agtgggagat
      421 gtcccgctcg tgccagaggg cgatattctc caaagccacc tgcgcctggc tgcgcaccag
      481 ccgcgccagc ccgcagagcc tttcgcaggt gaccgggttc ttcttgtgcg gcaaggccga
      541 actccccttc tgacccgcgg cgaacggttc ttcgacttcg cctacttcgg tccgctggag
      601 gtggcggatt tcggtggcga ttttttcgac cgtggccgcg atcaaagcca gcgtgccgag
      661 gtattcggcg tgccggtcgc gggagaccac ctgcgtggcg acgaccgaag gtttgagccc
      721 caatttttcg cagacgtagc gctcgacttc gggatcgacc ccggcgaaat tccccaccgc
      781 gccggaaatt ttccccacgg ccaccgcctc aatcgctcgc tccatccgca cctgctggcg
      841 cttgatctca tcgtaccacc ccagaagttt cagcccgaag gtggtcggct cggcgtgcat
      901 cccgtgggtg cggccgatca tccgggtgcg cttgtagcgg agcgccagac gcttgagcgt
      961 gtgaactgct ttctgtaaat cggcgacgat ttgctttccc gcttgcacga gcagataggc
     1021 ggtggcggta tccaccacgt ccgacgacgt gaggccgtag tggatgaagc ggctttcctt
     1081 gcccaccgaa agggcgacgt tagtgaggaa agcaatgacg tcgtgcttga ctgtcgcttc
     1141 gatcttctcg atggcggcca cctcaaagcg tgccttttgt ttgataatcg gcaccgcctc
     1201 cgccggaatg actcccaact gcgcctgcgc ctcgcaggcc aggatttcga tttggagcca
     1261 ggttttgaat ttggtctctt ccgaccagag tttggccatc tccggccggg tgtagcgggc
     1321 aatcataagc gcaagaaccg ctccctatcc gcgccccacc gccgtgggaa cacgggcttt
     1381 ttctttttta accgccgccg cttccggcag cgctttgcgc gccttttccc agtcggccag
     1441 gaaattctta agtcccacgt cggtcagcgg gtgtttcatt aactggtcta aaactttgaa
     1501 cggacaggtg gcgatgtcag cgcccatttg cgccgcgcgc gtgacgtgca gcgggtgccg
     1561 cacgctccct accaacactt cggtggggta gtcataattg tcgtaaatct cgatgatctg
     1621 ctgcaccacc tccatcccgt catggccgac atcatccagc cgccccacga acggggaaat
     1681 gatagaggcc ccgcacttgg cggccaaaag cgcctgattg gcggaaaaaa ctaaagtgac
     1741 gttggtgcgg attccttcgg cgctgcaggc cttgatcgct ttgagccctt ctttgattaa
     1801 gggaattttc accacgatat ttttgtggag tttggccaat tgccgggctt cgcgcataat
     1861 ggcgtcgaac tccaccgaca ccacttcggc ggagatggag ccgtcaacga tccggcagat
     1921 ttcccggaga atctcctcga agccggcttt ctctt
//
LOCUS       DBNGZA010000162         7127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1302402, whole genome shotgun sequence.
ACCESSION   DBNGZA010000162 DBNGZA010000000
VERSION     DBNGZA010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1302402"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /gene="argC"
                     /locus_tag="AC16FU_03415"
     CDS             <1..1027
                     /gene="argC"
                     /locus_tag="AC16FU_03415"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR2993456.1"
                     /translation="VVMKRVSVVGAAGYVGAELCALLARHNGVKLVGVSSSSEGEEGD
                     FASLHPALARREGPPVQRFQLETLLGNSPDCAFLAIPHETSAQLVPRLLAQGLTVIDL
                     SGAYRLKDVSGYPQWYEFSHPCPELLPDAVYGLTEWCDGRLPGCRLVANPGCYATSIL
                     MALKPLTPFLEFQEPLICDSKSGISGAGKRKELSYSFTELSGNFRAYSAGRHRHEPEI
                     RQELGLSTDTPLVFVPHLLPVIRGILSTLYVSFHKPVTVAMVSEAYHRAYVHSPFVQV
                     HPAGELPELKAVVGTPRMEIGFVLLSGGRRAVIISVLDNLLKGAASQAVQNFNHVFGF
                     DETEGLI"
     gene            1024..1815
                     /gene="argB"
                     /locus_tag="AC16FU_03420"
     CDS             1024..1815
                     /gene="argB"
                     /locus_tag="AC16FU_03420"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR2993457.1"
                     /translation="MNSLSALTVVKLGGSLLEDASLRAATLSAIARRWRAGASLVLVH
                     GGGREVDLRLAQLGIPKRTHQGLRITDAETLDEVISVLAGLVNKSLVAEFCARGVVAA
                     GFSGVDGATLRAEFHPRMDEVDLGFVGRVVAANPTLLKSVLKAGMLPVVASIAMGPQG
                     FLLNLNADAAASALAVSLRARRLVFLTDVEGLLDESGRVVGKLTASEACQLLDSNVVN
                     GGMRPKLAACLEAISDGVTEVVIAGPNTYADTLARGRGGTHLAPA"
     gene            1802..2998
                     /locus_tag="AC16FU_03425"
     CDS             1802..2998
                     /locus_tag="AC16FU_03425"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR2993458.1"
                     /translation="MLPLDESPAVVASREQTFLVRTYDRTPFHPRRGKGARLFDAQGR
                     AYWDLLAGIAVNVLGHQYSRLVKTLRAEAKNLLHVSNLFYHPAQGLLAEHLVRASGLS
                     RVFICNSGTEANEAALKFARLKHPGRPDVVALEESFHGRTLGALSLTGHAAYRMPFEP
                     LVPGVRFVPPNDIESLEKTVTEKTSAIFLEPILGEGGIIPLSSAYLKSARRIADATGA
                     LLILDEVQCGLGRTGALFAFQRAGILPDMVTLAKPLGGGLPLGAVLTGPALEGIVKPG
                     QHGTTFGGNPLACRLGLVVLEEIENSGLVKNVEQLGHWLGRELLKLKKRCSAIVDVRG
                     TGLMWGIELDRDASPVARALLAQGFVVGTAQKRVIRLLPPYVVPKTALRSFVKALEKV
                     LKESER"
     gene            2995..4266
                     /locus_tag="AC16FU_03430"
     CDS             2995..4266
                     /locus_tag="AC16FU_03430"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR2993459.1"
                     /translation="MKKTVKKAALAYSGGLDTSIIIPWLKENYGCEVVAVSVDVGQKE
                     EISGLAEKAYRTGASEFHLVDAREEFARDYLVSVLKAGAVYEKHYLLGTATARPLIAR
                     KQVEVALATGCDALAHGCTGKGNDQVRFELAYQALAPELTVIAPWREWAISSREAAID
                     YAAARGIPVPATKQDPYSRDRNLWHLSHEGGPLEDPASEPDPALYQLTVAPEAAPDEP
                     EKVSISFEAGVPVAVNGQQFTPAQLIESLNALGGKHGVGRADLVENRLIGIKSRGVYE
                     TPGGTILVTALPALESLTLDRDSAHEKERLALRYAELVYFGQWFSPLREALDAFVEKL
                     METVTGTVTLKLYKGTVSVLGRRSPYSLYSSHLASFNMAGYDAGDATGFIRLFGLPTR
                     GRKRVAPFVLSAKRTLRVMRNEVGSQEAGIR"
     gene            4263..5639
                     /gene="argH"
                     /locus_tag="AC16FU_03435"
     CDS             4263..5639
                     /gene="argH"
                     /locus_tag="AC16FU_03435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR2993460.1"
                     /translation="MTAPRIWGGRFQESPAENLKRFNDSFSCDHVLLNEEIEGSIAWA
                     RALAGAGVLSHSEVKKIVQGLVEIRKEGYAKPPDTSAYEDVHSFVEAALGVKVGVLAG
                     KLHSGRSRNDQVATDLKLFLRKALAATREQVLDLASALVRRSFEEAGTPMPGYTHLKQ
                     AEPITFGHWCLAYVEMLLRDASRLAGAVKRGDECPLGSGALAGTPLRIDRGRLAQALG
                     FARPTANSLDAVSDRDMAVEFLFAGSLLLVHLSRLAEDVILFSSDEFGFLELPDSLAT
                     GSSRMPHKKNPDVLELVRGNAARAIGDLAGLLSLLKGLPLSYNKDLQLDKEPVFRLHR
                     TLGLALPVLTDLVEKLRLDRNRMCRMAAQDNFLATELTDILAARGIPFRQAHEIVGRR
                     YAEARRAGKTLVEHGPAGKIAAGDLAALRVEKILAKKRAWGGTAPARVKEAAQAARKR
                     IQKLRRRP"
     gene            5636..6817
                     /gene="argJ"
                     /locus_tag="AC16FU_03440"
     CDS             5636..6817
                     /gene="argJ"
                     /locus_tag="AC16FU_03440"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR2993461.1"
                     /translation="MRLPKGFLASGVRAGIRRKHPDLALIVAEEGATAAALFTKNRFR
                     AAPVVLSQTALRKTGGHVKGVVINAGCANAITGNQGLQAARRVRARAATLLRCSPEQI
                     FLASTGIIGVVLADAKIRAALPAAVLRLSASGLASASQAILTTDVAPKVAQANFQWAG
                     RRGRLVGLAKGAGMIHPNLATMLAFLLTDAPATPRFLKAALRKAVDQSFHTISVDGDT
                     STNDTVLLLASGGLGGPTMTSLGQAADFCRALTEVCKALALMIVRDGEGATRLLEVNI
                     CGARTEREAHLAAHAVATSPLVKTALFSGDPNWGRVLAAVGRSGARFSPRRVGLSVGD
                     LPLVSDGEPAPYRETDAARLFSKERVCVRVNLGAGKAAATRITCDLGHDYVSLNGDYR
                     T"
BASE COUNT         1354 a         2039 c         2241 g         1493 t
ORIGIN      
        1 tgtggtgatg aaacgagtct cggttgtcgg agcggccggc tatgtgggag ccgaactctg
       61 cgcattgttg gcgcgccaca acggggtgaa gctggtcgga gtctcttcaa gttctgaggg
      121 agaggaagga gactttgcgt ctctccatcc cgcactggcg cggcgggaag ggcctccggt
      181 gcagcggttc cagttagaaa cgctcttggg caattctccc gactgtgcgt tcctggcgat
      241 tccccacgag acatcggcgc aactggtgcc ccgtcttctg gcccaaggtc ttacggtcat
      301 tgatctctcg ggtgcctatc gcctgaagga cgtcagcggg tacccacagt ggtacgagtt
      361 ttcccatccc tgcccggagc tgctccccga cgccgtctac ggtctcaccg agtggtgcga
      421 cgggcgactt ccaggttgcc gtctggtggc aaatcccggc tgctatgcca catcgattct
      481 catggcccta aaacccctta ctccgtttct ggaatttcag gagcctttga tttgcgattc
      541 caagagtggt atctccggag caggcaagag aaaggagctt tcttattcgt tcacggagct
      601 gtcggggaac ttcagggcct acagcgccgg aaggcaccgc catgagccgg agatccggca
      661 ggaattggga ctctcaaccg atacaccgtt ggtgtttgtt cctcatctcc tgcccgtcat
      721 ccgcggaatt ctttcaacgt tatatgtgag ttttcacaaa cccgtcaccg tcgcaatggt
      781 gtcggaagcg tatcaccggg cctacgttca ctcgcccttc gtccaggtac atccggcggg
      841 ggagctacca gagctgaaag cggtcgtcgg gacgccgcgg atggaaatcg gatttgtgct
      901 cctttcgggc ggccgtcgcg cggtgattat ttccgttctg gacaacctcc tcaagggcgc
      961 cgcttcccag gccgtgcaga actttaacca cgtgttcggc tttgacgaaa ccgaggggct
     1021 gatctgaata gtctcagcgc tctcaccgtc gttaagctgg gcgggagcct cctggaagac
     1081 gcatccctgc gggcggcaac gctctccgcg attgcccgca ggtggcgtgc gggcgcatcg
     1141 ctcgtactcg ttcacggagg cgggcgggag gtcgatttga ggcttgctca gctgggaatt
     1201 ccgaagagaa cgcaccaggg attacggatt actgatgcgg aaaccctcga tgaagtcatc
     1261 tccgttctcg cggggctggt gaataagtcg ctggtggctg agttctgtgc ccgcggtgta
     1321 gtcgcggctg gcttctccgg tgtagatggt gctacgctgc gcgccgagtt tcatccgcga
     1381 atggacgagg tggatctggg gttcgtgggg cgggtggttg ctgctaaccc cacgctgctc
     1441 aaaagcgtct tgaaagccgg gatgctcccc gtggtggctt ccatcgccat gggaccgcag
     1501 gggtttctgc tcaacctcaa tgctgacgcg gctgcctcgg ctctggctgt ttccctgcgg
     1561 gctcgcagac tggtgttttt gactgatgtg gaaggcctgc tggatgaaag tggacgcgtt
     1621 gtgggaaagc tgacggcgtc tgaagcttgt cagttgcttg actcaaacgt cgtgaatggc
     1681 ggcatgcgac ccaaactggc tgcctgtctg gaagccatct ccgacggcgt gaccgaagtc
     1741 gtcattgccg gacccaacac gtatgcagac actcttgccc gcggtagagg agggacgcac
     1801 cttgctcccg cttgacgaat cgccggcggt cgtggcatca cgagagcaaa ccttcctcgt
     1861 gcgaacgtat gaccgaaccc cgttccatcc ccgccggggt aaaggggcca ggcttttcga
     1921 tgcccagggc cgagcctact gggacctttt ggcgggcatt gccgtcaacg tgctggggca
     1981 ccaatattcg cggcttgtga aaactttacg ggccgaagcc aagaatctcc tccacgtctc
     2041 taatcttttc taccatccgg ctcagggcct tctggccgaa catttggtca gagcttctgg
     2101 tttgtctcgc gtcttcatct gcaacagcgg tacggaggcg aatgaggccg cactcaagtt
     2161 tgcccgactc aaacacccgg ggcggccgga cgtggtggcg ctggaggaaa gttttcatgg
     2221 ccggacgctg ggcgcactgt cccttaccgg acacgcggcg taccggatgc cgttcgaacc
     2281 gctcgtcccg ggtgtacggt ttgtcccgcc caacgacatt gaatctctcg aaaaaaccgt
     2341 gacggaaaag acgtcagcca ttttcctgga gcccattctc ggtgaggggg gaatcattcc
     2401 gctttcgtct gcctacctca agtctgcccg caggattgcg gacgcaaccg gagcactgct
     2461 catcttagac gaggttcagt gcgggctcgg tcggacaggg gcgctgtttg cctttcagcg
     2521 agcgggcatc ctgcccgata tggtcacgct ggccaagccg ttggggggcg gtctgccgct
     2581 gggagccgta ctgaccggac cagctctgga aggtatcgta aaaccaggcc agcacggaac
     2641 gacgttcggc ggcaatccgc tcgcctgccg actgggtctg gtcgtgctcg aagagattga
     2701 aaactcgggg ctggtgaaga acgttgagca gctgggccac tggctgggcc gggagcttct
     2761 caaactgaaa aaacgctgct cggccatcgt tgacgtgcgt ggcaccggtc tgatgtgggg
     2821 gatcgaactt gaccgggacg cctccccggt tgcccgggcg ctcctggctc aaggtttcgt
     2881 cgtcggaacg gcgcagaagc gcgtcatccg gttgctcccg ccgtacgtgg ttcccaaaac
     2941 agcgttgcgc tcattcgtca aagcgctgga gaaggtttta aaggagagcg agcgatgaaa
     3001 aaaacggtaa agaaggcggc cctggcgtat tcaggcgggc tggatacgtc catcatcatc
     3061 ccctggctca aggagaacta cggctgcgag gtcgtggccg tctccgttga cgtgggacaa
     3121 aaagaagaga tatccggtct ggccgagaag gcctaccgca ctggtgcgag cgaatttcac
     3181 ctcgtagacg ctcgcgaaga gtttgcccgg gattaccttg tttcagttct taaggctggc
     3241 gcggtctacg agaaacacta ccttttggga accgccacgg cacgtccgct cattgcccga
     3301 aaacaggtgg aagtagcgct cgccaccggg tgcgatgccc tggcgcacgg ctgcaccggg
     3361 aaagggaatg atcaggtgcg ttttgagctg gcgtaccagg cactggctcc ggaactcacc
     3421 gtaattgcgc cgtggcgcga gtgggcgatc agctctcgcg aagcagcgat cgactacgcg
     3481 gccgcacggg gaattccggt ccccgccacc aagcaggatc cgtattcccg ggatcgcaac
     3541 ctctggcacc tttcgcacga gggcgggccg ctggaagatc ccgccagtga acccgacccg
     3601 gccctgtacc agctgaccgt cgctcccgaa gcggcgccgg atgaaccgga aaaggtttct
     3661 atctcctttg aagcaggagt gcccgttgcg gtcaatggcc agcagtttac gccggcccaa
     3721 ttgatcgaat cgctcaacgc gctgggtgga aagcacgggg tggggcgcgc cgatctggtc
     3781 gagaaccggt tgattggcat caagtcgcgc ggcgtgtatg aaacgccggg agggacgatc
     3841 ctggtgacgg ccctcccggc gctcgaatca ctcaccctcg accgggacag tgcccacgaa
     3901 aaagaacggc tggccttgcg ctacgccgaa ctcgtctact ttggacaatg gttttcgccc
     3961 ttgagagagg cactggatgc gttcgtggag aagttgatgg agacggtcac cgggacggtg
     4021 acgttgaaac tctacaaggg aacggtgtcg gttctcgggc ggcggtctcc gtactcgctt
     4081 tattccagcc acctcgcgtc gttcaacatg gcgggttacg acgccgggga cgcgacggga
     4141 ttcatccggc tctttgggct gccaaccaga gggaggaagc gagtggcccc tttcgtgttg
     4201 tctgccaaga ggacactgcg ggttatgcga aacgaagtcg gcagccagga agcagggata
     4261 aggtgaccgc tcctcggatc tggggagggc ggtttcagga atctcccgcg gagaacttga
     4321 aacggtttaa cgattctttc tcttgcgacc acgttctgct caacgaagag atcgaaggat
     4381 cgatagcctg ggcccgcgcg ctggcggggg cgggagtcct ttcacactcg gaggtcaaaa
     4441 agattgttca ggggctggtg gagattcgga aagaagggta tgccaaacct cctgacacga
     4501 gcgcctacga agacgttcac tcgttcgtcg aagcagcact gggggtcaag gtcggggttc
     4561 tggccggaaa actccattcc ggtcgctcac ggaacgatca ggtggcaacc gacctgaaac
     4621 tttttctgcg gaaggcgctc gctgcaaccc gcgaacaggt gctggacctc gcctcggccc
     4681 tcgtgcgtcg ttcattcgag gaggcaggca cccccatgcc cggctacacc cacctgaaac
     4741 aggccgagcc gatcaccttt ggccactggt gcttggcgta cgtggaaatg ctcctgcgag
     4801 atgccagccg gctggccggg gcagtgaaga ggggagacga gtgtcctctt ggttcgggcg
     4861 ccctggccgg aactcccctg agaatcgacc gggggagatt ggcacaagcg ctgggttttg
     4921 cccggcccac ggccaattcg ctggatgcag tttctgaccg ggatatggcc gtcgagtttc
     4981 tctttgcggg tagtctcctc ctggttcatc tctcgcgcct cgccgaggat gtgatccttt
     5041 tttcttccga tgagtttggc tttctggaac ttcccgattc gctcgcaacc ggttcgtccc
     5101 gaatgccgca caaaaagaat cccgatgtgc tggagctcgt gcgcgggaac gcggcgcgtg
     5161 cgatcggcga cctggccggg ctgctttcgc tcttgaaagg gctgccgctc tcctacaaca
     5221 aggatctcca gctcgacaag gagcccgttt tccggctgca ccggacactc ggactggcct
     5281 tgccggtgct gaccgacctg gtagaaaaat tgaggctcga ccggaatcga atgtgccgta
     5341 tggctgctca agataatttt ctggccacgg agctgactga catcctggcc gcgcggggaa
     5401 ttccctttcg ccaggcgcac gaaattgttg gccgccggta tgccgaggcg cgacgagcag
     5461 ggaagaccct ggtcgaacat ggcccagcgg gtaaaatcgc ggcaggagat ctggccgccc
     5521 tgcgtgtcga gaaaatttta gcgaagaagc gggcctgggg cggaacagcg cctgcgcgtg
     5581 tcaaggaagc cgcgcaggca gcccggaaac ggattcagaa actccggagg cggccgtgag
     5641 actgcccaaa ggttttctcg catccggcgt gcgcgccgga atccggagaa aacacccgga
     5701 cttagcgttg atcgtcgcgg aagaaggtgc cacggcagca gcactcttca cgaagaaccg
     5761 ctttcgggcg gctccggtgg tgctttccca gaccgccttg cgaaagaccg gcgggcatgt
     5821 gaaaggagta gttatcaacg ctggttgcgc caatgcgatc accggaaatc aagggctcca
     5881 ggcagcccgc agggttcggg cgagagccgc gacactgttg cggtgcagtc cggaacagat
     5941 ctttctggct tctaccggaa tcatcggtgt cgttctggcc gacgccaaga taagagccgc
     6001 tctgccggcc gcggtgctgc gactttctgc ttcaggtctc gcctccgcct cgcaggccat
     6061 tcttaccaca gatgtggccc ccaaggtggc tcaggcaaat tttcaatggg ctggaaggag
     6121 gggccggctg gtaggcttgg ctaaaggggc aggtatgatt caccccaact tggctaccat
     6181 gctcgcgttc cttttaaccg atgctccggc taccccgagg ttcttgaagg ccgctctccg
     6241 aaaagcggtc gatcagagtt ttcacaccat ttcggtcgat ggcgacacgt ccacgaacga
     6301 tacggttctg cttttggctt ccggtggact gggaggtccc acgatgacgt cacttggcca
     6361 agcggcggac ttctgccggg ctttgactga ggtctgcaag gcgctggcct tgatgatcgt
     6421 ccgcgacggt gagggcgcca ctcggctgct ggaggtcaac atttgtggtg cgcgaacgga
     6481 gcgggaagcc cacctggcgg ctcatgccgt ggccacatca ccgctcgtga agaccgcact
     6541 tttcagcggc gatcctaact ggggacgggt tctcgcagct gtcgggcgca gcggcgcacg
     6601 gttttcaccc cgtcgtgtcg ggttgtcggt aggtgatctg cccttggtat ccgacggtga
     6661 gccagcaccc taccgtgaaa ccgatgccgc ccggctcttt tccaaagagc gtgtttgtgt
     6721 cagggtgaat ctcggtgccg gcaaagccgc agccacccgc ataacgtgcg atctgggcca
     6781 cgactacgta tcgctcaacg gggattaccg gacttagaga gttgccggaa aatagcttca
     6841 taacattcat tgccgcaggt caaagttatc tggctgctgg cgaaaacaat tgttgcggta
     6901 tggaggcgga agtcattatc gcctctcaaa ttttctcgtg gggccgcagg cgaaccggta
     6961 cttttgcttg gtcagacaag gtatagctgt gagtactact tcccgagaga atcattgcaa
     7021 atctgagcaa ttggaggatt gggaagttag gaacgcgtca ataattttcg tgtacttact
     7081 gcaggaattt acgtgtccgg cttcccactt ccttaccgta ctctcgt
//
LOCUS       DBNGZA010000163         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_453952, whole genome shotgun sequence.
ACCESSION   DBNGZA010000163 DBNGZA010000000
VERSION     DBNGZA010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_453952"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1071)
                     /locus_tag="AC16FU_03445"
     CDS             complement(67..1071)
                     /locus_tag="AC16FU_03445"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR2993462.1"
                     /translation="MKIQAEILELRLKHTFRIASGATDLKRNVIVHIGEGIGEAAPAL
                     YLGEWYKSVLACLEFLEPELGDDPALIEEIEDRISQKIGFNFAAKAAIDLALHDHLSK
                     KAGVPLFQFLGLTKSEEKKTSVTIAIAEPKEMAQRAVEAKTFPILKVKVGVPGDMEKV
                     TAVRRARPDAAIRVDANCGWTLVEALEKMPLLEQLSVELVEAPLDSVDIEGLRLLRTK
                     SPLPIFLDEGVMTAKDIPGYAPVADGINVKLMKCGGLREALRMIREARRLNLKVMLGC
                     MLESAIGVTAAAHLAGLADYLDLDSILLTDNDPYQGAAIENGVLRLPQRPGIGVVKVG
                     "
     gene            complement(1068..2033)
                     /locus_tag="AC16FU_03450"
     CDS             complement(1068..2033)
                     /locus_tag="AC16FU_03450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR2993463.1"
                     /translation="MPRTPGKKLLRPRALEIGGTIGIVAPGSPPGRRRLRAGVAFLKQ
                     QCFKVKIHSQCYRSFGFLAGRDEVRAQALHAMFADPDIDAILCARGGVGCLRLLPLLD
                     FGLIRRNPKIFVGYSDLTALHLAIYQRCRLVTLHGPMVASELGPKVPHFTARHLFRSL
                     RDGRPPGVVSIGGKRKPFTIIRPGCASGPLLGGNLSLVCRLVGSGYLPDFAGALLFLE
                     DINEEPYRIDNFLSQLRLSGILNHVAGVVFGNFVDCEPEDAEKPSFSLREVLFDHFSH
                     MPYPVIWGVPFGHGKKTITIPHGVQGTVDTRARRFSIDEAAVTSA"
     gene            complement(2038..>2411)
                     /locus_tag="AC16FU_03455"
     CDS             complement(2038..>2411)
                     /locus_tag="AC16FU_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HIR2993464.1"
                     /translation="IAFLKEVPSGTPISYGRTFTTERPTLVATLPLGFADGFPRQLSN
                     RGAVLVRGKKAPVIGRVCMDAFMVDVTGIVGVKVGDEVVLVGWQGKEAIDLWALASAI
                     DTTAYEVVSRLGKRLPVDYRL"
BASE COUNT          526 a          736 c          676 g          473 t
ORIGIN      
        1 ccgatctgag cgcggccagt cgacacagca agagagaaaa ttaaaaagag aagaaagacc
       61 gaacgcttag ccaactttca ccaccccgat ccccggtctt tgcggcaagc gcaagacacc
      121 attctcaatc gctgctcctt gatagggatc attgtcggtc aaaaggatgc tgtctaagtc
      181 gaggtaatcg gccagaccgg ccaggtgtgc cgcagccgta actccgatag cactttccag
      241 catgcacccc agcataactt tgaggttcaa ccgccgggct tcgcgaatca tccgcagcgc
      301 ctcgcgcagg ccgccgcact tcatcagttt gacgttgatg ccatccgcca ccggcgcgta
      361 gcccggaatg tctttggccg tcatcactcc ttcatccagg aagatcggca ggggcgattt
      421 ggtgcgcaag agccgcagcc cctcgatatc gacagaatcc aaaggcgctt ccaccaattc
      481 aacgctcaac tgctccaata gcggcatctt ctctaacgct tccaccaagg tccagccgca
      541 gttggcatcg acccggatcg cggcatcggg ccgcgcccgg cggactgcgg tgaccttttc
      601 catatctccg ggaactccga ccttaacctt gagtatcgga aaagtcttcg cttccactgc
      661 ccgttgagcc atctcttttg gctcggcaat ggctatggtc accgaggtct ttttttcttc
      721 ggatttcgtc aatcccaaaa actgaaagag cggcacgccc gcttttttgc tcaggtggtc
      781 gtggagggcg aggtcaatgg ccgctttcgc cgcgaagttg aaaccgattt tctgactgat
      841 gcgatcttca atttcctcga tcaatgcggg gtcatcaccc agctccggct ctaaaaattc
      901 caggcaagcc aagaccgatt tataccactc accgagatac agtgcgggcg ctgcttcgcc
      961 gatgccctcg ccgatatgga cgatgacgtt gcgcttgaga tcggtcgccc cgctggcaat
     1021 gcggaaagtg tgtttgagtc ttaactccag aatttcggcc tgaatcttca tgcgctggtg
     1081 accgccgcct cgtcgatgga aaagcgtctc gctctcgtgt cgaccgtccc ctgcacgccg
     1141 tggggaatgg tgatcgtttt tttgccgtgg ccgaagggca caccccagat gaccgggtag
     1201 ggcatgtgcg agaagtggtc aaacaacact tcgcgcaaag agaaagacgg tttctctgcg
     1261 tcttccggct cgcaatcgac gaaattacca aacaccactc ccgctacgtg gttcaagatc
     1321 cctgacagtc tcaactggct taggaaatta tcaatgcggt agggctcttc gttgatgtct
     1381 tccagaaaga ggagcgcacc ggcaaagtcg ggcaggtagc ccgagccgac aagccggcag
     1441 actaaagaga ggttcccgcc caaaagcggg ccggaggcgc agccggggcg gattatcgtg
     1501 aacggttttc gcttgccgcc gatggacaca acccctggcg gccttccatc cctgagcgag
     1561 cgaaaaagat ggcgcgcggt gaagtgcggc acctttggtc ccaactccga ggccaccatc
     1621 ggcccgtgca gggttaccag tcggcagcgc tggtagatgg ctaggtgcaa ggcggtcaga
     1681 tcagagtacc cgacaaaaat tttgggattt ctccgtatga gaccgaagtc gagcagcggg
     1741 agcagccgca gacagccgac gccgccgcgc gcgcagagaa tcgcatcgat atccgggtcg
     1801 gcaaacatcg cgtgcagggc ctgcgccctg acctcatcgc gaccggccag aaagccaaaa
     1861 ctgcggtaac actgcgagtg gattttgact ttgaagcact gctgcttaag gaaagcgact
     1921 cccgctctca ggcgcctgcg gccgggcggg ctgccgggcg ccacaatccc aatggtcccc
     1981 ccgatttcaa gcgcgcgcgg gcgaagcaac ttcttccctg gagttcgggg catcagatca
     2041 caagcggtaa tccaccggca aacgcttgcc caggcggctt accacttcgt aggcggtggt
     2101 gtcaatggcg gaagccagcg cccaaagatc aattgcttct tttccctgcc acccgaccaa
     2161 aaccacttca tcgccgactt ttactccgac aatgccagtg acatcgacca tgaaggcgtc
     2221 catgcagacc cggccgatca ccggggcttt cttcccgcgc accagaaccg cgccgcggtt
     2281 ggaaagctga cgcgggaatc catcggcgaa accgagggga agcgtggcga cgagcgtcgg
     2341 ccgttcggtg gtgaacgtgc ggccgtagct gatgggagtc cccgacggca cttccttgag
     2401 gaaagcaatc c
//
LOCUS       DBNGZA010000164         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_454158, whole genome shotgun sequence.
ACCESSION   DBNGZA010000164 DBNGZA010000000
VERSION     DBNGZA010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_454158"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC16FU_03460"
     CDS             <1..498
                     /locus_tag="AC16FU_03460"
                     /inference="COORDINATES: protein motif:HMM:NF026044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993465.1"
                     /translation="ILHIDFQHITLTRKITVNIPVHVVGLAVGVKDTGGILQHTLREL
                     AVSCLPTEIPEKVDVDVTALKIGDSIHVRDIKIEKAEILNDPNSSVVSVVPPTIIKEE
                     ELAAAAPAAEVTEPEVITEKEAQERAAAKAEAEKVEGKAEKKAPPTAGEAKAEKKEEK
                     KSEKK"
     gene            606..1361
                     /locus_tag="AC16FU_03465"
     CDS             606..1361
                     /locus_tag="AC16FU_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2993466.1"
                     /translation="MKGVILAGGLGSRLFPLTKITNKHLLPVYDQPMIYYPIQTLVEA
                     GIKDILIVTGGNSAGDFLRLLGNGKEFGLKHIDYTYQEKEGGIAEAIGLAEHFAEGEK
                     ILVMLGDNIIFESIKEAVEAFKFQKRGAKIFLKPVKDPEAYGVAELKTGKVTRIVEKP
                     RRPKSNYAVVGIYMYDNQVFEIVKTLQPSGRGELEVTDVNNAYLAQGELTHAILRGWW
                     GDAGESIEALLEANRVVAEYRQNQAKVKRARKK"
     gene            1358..1882
                     /locus_tag="AC16FU_03470"
     CDS             1358..1882
                     /locus_tag="AC16FU_03470"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2993467.1"
                     /translation="MSFAVEQSVVIKRSLIPIWHFWIDLPQWPRWHTGLLDAYWLTSE
                     LWWKKGSQFVLVWQGKFPLNRYKVRCTVTSVEPGKSITWRAKGIGLKAMRGYRFSEVS
                     GGTQVTAYGKFNGPAAVLLRPLARAHIKMRLTQALAGLAKMVEQEVLVGKPKVERVEV
                     PPEWLVTGKKYLPF"
     gene            1905..>2070
                     /locus_tag="AC16FU_03475"
     CDS             1905..>2070
                     /locus_tag="AC16FU_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR2993468.1"
                     /translation="MIHGVKTKQLKVIPDERGRLMEVLRSDDKDFFKKFGQVYVTTAY
                     PGVVKGWHYHK"
BASE COUNT          520 a          540 c          648 g          362 t
ORIGIN      
        1 atcctgcaca tcgacttcca gcacattacg ctcacccgga agatcaccgt gaacatcccg
       61 gtccacgtcg tcggcttagc cgtgggagtg aaagacaccg ggggcatttt gcagcacacc
      121 ctgcgcgagc ttgcggtttc ctgtctgccg accgagattc ccgaaaaagt ggacgtggac
      181 gtcacggcgc tcaaaatcgg cgactcgatt cacgtgcggg acattaaaat cgagaaggcg
      241 gaaatcttaa acgacccgaa ttcctcggtg gtctcggtgg tgccgccgac cattatcaag
      301 gaagaggaac tggcggccgc agcgccggca gccgaggtga ccgagccgga agtgatcacc
      361 gaaaaagaag cccaggagcg ggcggcggcc aaggccgaag cggagaaggt cgagggcaaa
      421 gccgaaaaga aagccccgcc aacggctggg gaagccaaag cggagaaaaa agaagagaag
      481 aaatccgaga agaaatgatt ggggtcaggc agtcggttac gccggggtaa agccccggcg
      541 tttttgtttt gcggcgtggg tttgagtata ttcgcgaaaa atttcaagag atgcaaagct
      601 aaccgatgaa aggcgtcatt ctcgcgggcg ggctgggcag ccggctcttc ccgctcacca
      661 agatcactaa caaacatctt ttgccggtct acgaccagcc gatgatttac tacccgattc
      721 agacgctggt ggaagcgggg atcaaggaca ttctgatcgt caccggcggg aattcggcgg
      781 gcgatttcct ccggctcttg ggcaacggaa aagaattcgg gctcaagcac atcgactaca
      841 cctaccagga aaaagaaggc ggcatcgccg aggcgatcgg cctggcggag cacttcgccg
      901 aaggggagaa aattttggtg atgctggggg acaacatcat cttcgaatcg atcaaagagg
      961 cggtggaggc gttcaagttc cagaagcgcg gcgccaaaat atttctgaaa ccggtgaaag
     1021 acccggaggc ctacggcgtg gcggagttga agactggcaa ggtgacgcgc atcgtcgaaa
     1081 aaccccgacg gcccaagagc aactacgccg tggtcggcat ctacatgtac gacaaccagg
     1141 tctttgagat cgtcaagacg ctccaaccct cgggccgggg ggagttggaa gtaaccgacg
     1201 tgaacaacgc ctacctggcc cagggcgaac tgacccatgc catcctgcgc ggctggtggg
     1261 gggatgccgg tgagtcgatt gaggcgctat tggaagccaa ccgggtggtg gcagaatacc
     1321 gccagaacca agcgaaggtt aaacgtgcca ggaaaaaatg agtttcgccg tcgagcagtc
     1381 cgtcgtcatc aaacgctcct tgattcccat ctggcacttc tggatcgatc tgccacagtg
     1441 gccccggtgg cacaccggac ttctggacgc ctactggctg acatccgaac tctggtggaa
     1501 aaaaggctcg cagttcgtct tggtctggca gggcaaattt ccgctcaaca ggtacaaggt
     1561 tcgttgcacg gtcaccagtg tggaaccggg gaaaagtatc acctggcgcg caaaagggat
     1621 tgggctaaag gccatgcgcg gctaccggtt cagcgaagtt tcaggcggga cgcaggtgac
     1681 agcgtatgga aagtttaacg gcccagcggc ggtgctgttg cgacccctgg ctcgtgccca
     1741 catcaaaatg cggctgacgc aggcgctggc cggattggcg aagatggtcg agcaagaggt
     1801 gctggtggga aaacccaaag tggagcgggt cgaagtacca ccggagtggc tggtcaccgg
     1861 caagaagtac ctgcctttct aatggtagag aaacgaggtc aaaagtgatt cacggcgtca
     1921 aaactaaaca actgaaagtc atccccgacg agcgcgggcg cttgatggaa gttttgcgct
     1981 cggatgataa ggactttttc aaaaaattcg gccaggtcta cgtcaccacg gcctacccgg
     2041 gggtggtgaa gggctggcac taccacaagc
//
LOCUS       DBNGZA010000165         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_135220, whole genome shotgun sequence.
ACCESSION   DBNGZA010000165 DBNGZA010000000
VERSION     DBNGZA010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_135220"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="AC16FU_03480"
     CDS             complement(<1..168)
                     /locus_tag="AC16FU_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR2993469.1"
                     /translation="MDFHTGLALGVPIGLGLAAIGSGIGLGGAVRGAMEAMGRQPEAS
                     GKIKTGIIIGAA"
     gene            complement(196..1062)
                     /gene="atpB"
                     /locus_tag="AC16FU_03485"
     CDS             complement(196..1062)
                     /gene="atpB"
                     /locus_tag="AC16FU_03485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR2993470.1"
                     /translation="MSFTSLVHSSGLVSGGFWQEAGQPPAEVPEGAPELPNILTFVSH
                     LFGPQAAHFIHAYQNIIFAWLVMILLGVVAYLASRKSALIPGPLQNLMEMAVEELSNF
                     IIGILGPEGKRYVPFLGTIFLYTLAMNWFGLIPGLFSPTGGPNGVNITAAQAICVFLY
                     VQYTALTRLGFFKYLHHLAGEPKDAIGWSLVPINLPVHLIGELAKPFSLAIRLFGNIT
                     GEDILIAAFVGLGIALVAVLKSPVGIPLQVPFIFLALLTSTIQALVFTLLSTVYILLV
                     LPHHEEGEKREH"
     gene            complement(1063..1467)
                     /locus_tag="AC16FU_03490"
     CDS             complement(1063..1467)
                     /locus_tag="AC16FU_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993471.1"
                     /translation="MGLEFIQRIIKTTAVVATLAGLFVAVYYQFPYAVGLWLGALWGC
                     LNFFFIAALVKALLTPAQPIRWGNVIVTLGIKFPLLYGLGYLLLVWRYAPAVSYLIGF
                     TLLIAVTVLKALGRMLLGLDTGSLFHLTEMRK"
BASE COUNT          384 a          450 c          447 g          306 t
ORIGIN      
        1 cgccgcgcct atgataatgc cggttttaat cttgcccgaa gcttcgggct gccggcccat
       61 cgcctccatc gcaccgcgca cggcgccgcc caagccgatc cccgagccga tagcggctaa
      121 ccctaaaccg atcggtacgc ctaacgccag tcctgtgtgg aagtccattg tttctcctcc
      181 tgtgtgatct ggtggttaat gttcacgctt ttccccttct tcgtggtgcg gcaatacgag
      241 cagaatgtag accgtcgaaa gcaaagtgaa aactaacgcc tgaatggtgc tggtcaaaag
      301 cgctaagaaa atgaacggca cctgcagcgg gatgccgacc ggagatttga gtaccgctac
      361 gagcgcgatg cctaagccga cgaaagccgc gatcaagatg tcctcgccgg taatgttccc
      421 gaagagacgg atcgccagac tgaacggctt ggccagctcc ccgatcaggt gcaccggcag
      481 attgatcggc actaaggacc agccgatggc atctttcggt tcccccgcca ggtggtgcag
      541 gtatttgaag aaccccagcc gcgtgagcgc ggtgtactgg acgtagagga agacgcagat
      601 cgcctgcgcc gcggtgatgt tcaccccgtt gggtccgccg gtaggcgaga ataaaccggg
      661 aattaggccg aaccagttca tcgccagagt gtagaggaaa atcgtgccga ggaaagggac
      721 gtagcgtttt ccctccggcc cgaggatgcc gatgatgaaa ttggaaagtt cctctaccgc
      781 catctccatc aaattttgca gggggccggg aatcaaagcg ctcttgcgag aagcgaggta
      841 ggccaccacg cctaaaagaa tcattacgag ccaggcgaaa atgatgttct ggtaagcgtg
      901 gatgaaatgc gctgcctgtg ggccgaacag gtgagaaaca aaggtcagga tgttgggcag
      961 ttctggcgcg ccctccggca cttcagccgg gggttggcca gcctcctgcc aaaagccgcc
     1021 gctcaccaga ccgctactat gaaccaggct cgtgaacgac atttacttcc tcatctccgt
     1081 gaggtgaaaa agcgaccctg tatccagccc cagaagcatt cgtcccaggg ctttgagaac
     1141 cgtcaccgcg attaagagag tgaaaccgat taagtaactt accgccggcg catatctcca
     1201 gacgagcaaa agatacccca gaccgtagag cagcgggaat ttgatgccca gcgtgactat
     1261 cacgttgccc cagcgaatcg gttgtgccgg agtgaggagt gccttgacca gcgccgcaat
     1321 gaaaaagaaa ttgagacacc cccagagcgc accgagccac agacccactg cgtagggaaa
     1381 ttggtagtac acggccacaa ataagcccgc gagggtggcc accactgcgg tcgttttgat
     1441 gatcctttgg atgaattcta atcccatatt actcgggcgc gttcgctttc ttggcgattt
     1501 gatacacttc ccgcccggac gccacgaatc ccagaataat caacaggtac gtcaggtagg
     1561 ggtcagtgga aagtttcccg tccagaa
//
LOCUS       DBNGZA010000166         1476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1195771, whole genome shotgun sequence.
ACCESSION   DBNGZA010000166 DBNGZA010000000
VERSION     DBNGZA010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1195771"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="AC16FU_03495"
     CDS             complement(<1..886)
                     /locus_tag="AC16FU_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR2993472.1"
                     /translation="MSIRPVKWLIKAKPTLEGAGVHLRRAFGFGNTADFDPFLLLDDF
                     RNERPEDYLAGFPWHPHRGIETITYVLAGTVEHGDSIGNRGSIAAGDVQWMTAGSGIL
                     HQEMPKWDQGGRMHGFQLWANLPSALKMTAPRYQEVKSEEIPEITDDDGTRVRLVCGN
                     FWGKKGPVVGIAADPIYLDVSIPAGRRKTLPVETRRQAFAYVFAGAGKFCNASGPLDV
                     PTEGVGWADTKPPSEAENRSLVLFDTGDEVTIQAGEEGLRFLLASVEPLKEPVAWYGP
                     IVMKTQAELRQAYEELERG"
     gene            complement(1038..1265)
                     /locus_tag="AC16FU_03500"
     CDS             complement(1038..1265)
                     /locus_tag="AC16FU_03500"
                     /inference="COORDINATES: protein motif:HMM:NF040441.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HIR2993473.1"
                     /translation="MKCPSCNKEMEPGFMYVRGFGGSLLWSKRGDTRFFSRRGLEIID
                     LGRLSQTRTGAQAVLPAARCPSCATLAFKAA"
BASE COUNT          288 a          465 c          427 g          296 t
ORIGIN      
        1 ttccccgttc caactcctcg tacgcctgcc ggagttctgc ctgggttttc atcacgatcg
       61 ggccgtacca ggccaccggc tccttgagcg gttccacgga cgccaaaaga aaccgtaacc
      121 cctcctcccc ggcctggatg gtcacctcgt cgccggtgtc aaagaggacg agcgagcggt
      181 tctccgcctc ggaagggggc tttgtatccg cccacccaac cccctcggtc ggcacatcca
      241 gcgggccgga cgcgttgcag aacttccccg ctccggcaaa aacgtaggcg aaggcctggc
      301 gccgggtttc gaccgggagg gtcttccgcc ggcccgccgg aatcgacacg tcgaggtaga
      361 tcgggtcggc tgcgatcccc accaccggtc ccttcttccc ccagaaattt ccgcagacga
      421 ggcggacccg ggtgccgtcg tcatcggtga tttccggaat ttcttccgat ttcacctcct
      481 ggtagcgggg cgccgtcatc ttgagcgccg acgggagatt ggcccacaac tggaacccgt
      541 gcatccggcc cccttggtcc catttgggca tctcctgatg gagaatcccg ctccccgcgg
      601 tcatccactg aacgtcgcct gcggcaatgg agccccggtt cccgatgctg tcgccgtgct
      661 ccacggttcc ggccagaacg taggtgatgg tctcgatccc gcggtggggg tgccagggga
      721 agccggcgag gtaatcctcg ggccgttcgt tgcggaagtc gtccaaaagg agaaacgggt
      781 cgaagtcggc cgtgttgccg aatccgaacg cacggcgcag atgcaccccc gcgccttcca
      841 aagtcggctt ggccttgatc agccacttaa ccggtcgaat agacatggga atctcctctt
      901 ttatgatttg aggttgacgg ggacgggcat acgtacgcgt ccattttcat cctgtattta
      961 ctctaagtat aaaggcaaaa acaaagagag gcaaaacgtt caaggagact ccatttcttc
     1021 ggaagataaa gagccactca cgcggctttg aacgccagag tcgcgcaaga gggacagcga
     1081 gccgccggca ggacggcctg ggcgcccgta cgggtctggc tgagtcggcc caaatcgata
     1141 atttccaggc cccggcgcga gaagaaccgg gtgtcaccgc gcttcgacca gagtagagag
     1201 ccgccgaaac ctctgacgta catgaatccc ggctccatct ctttgttaca ggacgggcat
     1261 ttcatcggga accccttatg tcgatttaac gggcggctca cttgcagctg cagcgaagag
     1321 ttgggctgcc ccacttgcag gacgccgtga ccgataactg ttcatagttg agatcacctt
     1381 tcgagaaaga ctttcagcac ccgggcactg ttgtgttctg catcgtgtgc tgcgtacaca
     1441 aaggtaacgg ccttcttctc tcgcagcaat ccttgc
//
LOCUS       DBNGZA010000167         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_30895, whole genome shotgun sequence.
ACCESSION   DBNGZA010000167 DBNGZA010000000
VERSION     DBNGZA010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_30895"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16FU_03505"
     CDS             complement(<1..650)
                     /locus_tag="AC16FU_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR2993474.1"
                     /translation="MARKFPLARVRNIGIMAHIDAGKTTTTERILFYTGRSYRIGEVD
                     EGAAIMDWMEQEKERGITITAAATTCVWSDHKINIIDTPGHVDFTIEVERSLRVLDGA
                     VAIFCAVGGVEPQSETVWRQADKYGVPRIAYVNKMDRVGADFDNVLEMMRKRLGANPV
                     PVQLPVGQGDLFTGIIDLIDFTFRVYDDASQGVTFDDRPIPKDLQKPAHDAREKLL"
     gene            complement(845..1315)
                     /gene="rpsG"
                     /locus_tag="AC16FU_03510"
     CDS             complement(845..1315)
                     /gene="rpsG"
                     /locus_tag="AC16FU_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR2993475.1"
                     /translation="MPRRRQIERRLRLPDPKYQSPLVTQFVNNLMKRGKKSTAEKIFY
                     RAVDLAGEKSGQPGLDVFQKALSNVKPVVEVKSRRVGGATYQVPVEVRPERRTALGIR
                     WLIQYGGERPGKTLAAKLAEELLAAAKNEGGAVKKKEDTHKMAEANKAFAHYRW"
     gene            complement(1355..>1669)
                     /gene="rpsL"
                     /locus_tag="AC16FU_03515"
     CDS             complement(1355..>1669)
                     /gene="rpsL"
                     /locus_tag="AC16FU_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR2993476.1"
                     /translation="GSPQKRGVCTRVYTTTPKKPNSALRKVARVRLTNGMEVTAYIPG
                     EGHNLQEHSIVLIRGGRVKDLPGVRYHIIRGTLDAQGVGARHQGRSKYGAKKPKEAAA
                     KG"
BASE COUNT          265 a          544 c          468 g          392 t
ORIGIN      
        1 tccaaaagct tttcgcgcgc gtcatgggcc ggcttttgca aatccttggg aatcggccga
       61 tcgtcaaagg tgaccccctg gctcgcgtcg tcgtagaccc ggaaggtgaa gtcgattaag
      121 tcgatgatgc cggtgaagag gtcgccctgg ccgacgggaa gttgaaccgg caccgggttg
      181 gcccccagcc gtttgcgcat catctctaag acgttgtcga aatcggcccc cacccggtcc
      241 atcttattga cgtaggcgat ccgcggcacg ccgtacttgt cggcctggcg ccagaccgtt
      301 tccgactgcg gctccacgcc gcccaccgca cagaagatgg ccaccgcgcc gtccaaaacg
      361 cgcaaagacc tctccacttc gatggtgaaa tcgacgtggc ccggggtgtc gatgatgttg
      421 attttgtggt cgctccagac gcaggtggtg gccgccgcag tgatggtgat gccgcgttcc
      481 ttttcctgct ccatccagtc catgatggcg gcaccttcat ccacttcgcc gatccggtac
      541 gagcggccgg tatagaaaag aatcctttcg gtcgtcgtcg tcttcccggc gtcgatgtgg
      601 gccatgatgc cgatgttgcg tacccgtgct aacggaaatt tgcgtgccat aaaaaaatac
      661 tccactcccg cctgtgaaaa cccctctccc gtacgggcca agtggaggat ttgtctcact
      721 ccttctgggt tttcaaaaac tcgttacgtg cttatcacct ttacccgtac gggcacggtg
      781 ctggcgacct cctgtatttt ttctgcccta cgacctacga ccgctcaccc atcacctttt
      841 tcggctacca ccggtagtgc gcgaaggcct tgttggcctc ggccatcttg tgcgtatctt
      901 ctttcttttt gacggcgccg ccttcgtttt tcgccgccgc caacagctct tcggccagct
      961 tcgccgccaa agtcttgccg gggcgctccc ccccgtactg gatgagccag cgaatgccca
     1021 gagcggtgcg ccgttccggc cgcacttcca ccggcacctg gtaggtggcc cccccgaccc
     1081 ggcgcgactt cacctctacc accggcttga cgttggagag cgctttctgg aaaacgtcta
     1141 aacccggttg gcctgatttt tcgcccgcca ggtcgacagc ccggtagaaa attttttccg
     1201 cggtgctctt cttgccgcgt ttcatcaagt tgttgacgaa ctgcgtcacc aaaggactct
     1261 ggtacttcgg gtccggcagc cgcaggcgcc gttcgatctg tcgtctcctc ggcatctcgt
     1321 tcctcagtcc tcagggctct ggttcatttt cgattcatcc cttcgccgcg gcttccttgg
     1381 gctttttggc accgtacttg gagcggccct ggtgccgcgc acccacgccc tgggcatcca
     1441 gggtgccgcg gatgatgtgg tagcgcactc ctggcagatc tttgacccgg ccgccccgga
     1501 tgagcacgat ggaatgttcc tggaggttgt ggccctctcc gggaatgtac gcagtcacct
     1561 ccatcccgtt ggtgagccgg acccgcgcca ctttgcgcaa ggcggagttc ggcttcttgg
     1621 gggtggtggt atagacccgc gtgcagaccc cgcgcttctg gggcgatcc
//
LOCUS       DBNGZA010000168         1443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1832670, whole genome shotgun sequence.
ACCESSION   DBNGZA010000168 DBNGZA010000000
VERSION     DBNGZA010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1832670"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="AC16FU_03520"
     CDS             <1..825
                     /locus_tag="AC16FU_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR2993477.1"
                     /translation="AHRWIDFGPIQFQPSELFKLAVILYVAAALAKRGHKISDLRYLL
                     LPYAPLLGVAFILLMLEPDLGTFLVIAGIVFSSLAIAGARWKHLLTGFLAAALVVSVL
                     VVGLGYKQQRVKDYVKSWEDPLAGSYQVKQAMLGLSLGGLTGAGLGEGRQKMLYLPAP
                     HTDFIFAAIGEEGGLLWLALILALFFVLIWRGWLVAQQAPDRFGFSLAFGITALLFTS
                     ITLNVGVVLGLLPTTGIPLPFLSYGGTALLAASAGCGVLLNIASQKEMQARYGYVR"
     gene            809..>1443
                     /locus_tag="AC16FU_03525"
     CDS             809..>1443
                     /locus_tag="AC16FU_03525"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF015022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR2993478.1"
                     /translation="MATYGKLKILFAGGGTGGHLFPAIALAQAAEKAFPGVQVVFVGA
                     PQGLEAKKIPELGWRLHLVPIRGLSRTRPFSNLVFPFSVVRSLVQANRVLSLEQPDLV
                     VGSGGYVAWPVLRAAVAKSIPTLIQEQNSYPGIVTRRLASKVNAVCLAYAEAAAYLKR
                     QDNITVTGNPVRPDIASGKREEAVKVFGLDPAKKTVLVIGGSQGAASINRAL"
BASE COUNT          241 a          404 c          455 g          343 t
ORIGIN      
        1 gcgcaccgct ggatcgactt cgggccgatt cagttccagc cgtcggagct cttcaaatta
       61 gcggtcattc tgtatgtcgc ggccgcgctg gccaagcggg ggcacaaaat ttctgacctg
      121 cgctaccttt tgctgccgta cgcgccgctt ttgggagtgg cgttcatttt gctcatgctg
      181 gagccggatt tgggaacttt tctcgtcatc gcgggcatcg tcttttcatc tttggccatc
      241 gccggggcac ggtggaagca cttactcacc ggatttttgg cagcggctct ggtggtttcg
      301 gtactcgtcg tcggattggg ctacaagcag cagcgggtca aagactacgt caaatcctgg
      361 gaagatcccc tggccgggag ctaccaggtg aagcaggcga tgttgggttt gagtttgggt
      421 ggattgaccg gggccgggtt gggcgaagga cgccagaaga tgctctatct ccccgcgccg
      481 cacaccgact tcattttcgc cgcaatcgga gaagagggag gacttctctg gctggcgctc
      541 atcttagcgc tgttcttcgt tttaatctgg cgcgggtggc tcgtcgccca gcaggccccg
      601 gatcgttttg ggttttccct ggcgttcggc attacggctt tacttttcac cagcatcacc
      661 ttaaacgtcg gcgtcgtttt aggcctttta cccaccacgg gcattccgct gccctttttg
      721 agttacggcg gcacggcgct attggctgcc tccgccgggt gtggtgtgct cttaaatatt
      781 gcgagtcaga aggagatgca ggcgcggtat ggctacgtac ggtaagttga aaattctttt
      841 tgccggcggc ggcaccggcg ggcatctctt cccggcgatt gctctggcgc aggcagcaga
      901 gaaagccttc cccggcgtcc aggtcgtttt cgtcggcgca ccgcaggggc tggaagccaa
      961 aaaaattccc gagttgggct ggcgcttgca cctcgtgccg attcgcggtc tctcacgcac
     1021 acgtccgttc tcgaatttgg ttttcccgtt ctccgtagtg cggtcgctgg tacaggcgaa
     1081 tcgcgtcctt tcgctggagc aacccgacct cgtggtgggc agcggcggct atgtggcctg
     1141 gccggttttg cgcgccgcgg ttgccaagag tattccgact ttgattcaag agcagaatag
     1201 ctacccgggt atcgtgaccc ggcgcttggc gtcgaaggtc aacgcagtct gcctggccta
     1261 cgccgaggcg gcggcctatc tcaaacggca ggacaacatc accgtcaccg gcaatccggt
     1321 gcggccggat attgcgtcgg gtaaaaggga agaagcggtc aaagtttttg gactggatcc
     1381 ggcgaagaaa acggtgctgg tgatcggcgg cagccaggga gcggcctcca tcaatcgggc
     1441 gct
//
LOCUS       DBNGZA010000169         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_667186, whole genome shotgun sequence.
ACCESSION   DBNGZA010000169 DBNGZA010000000
VERSION     DBNGZA010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_667186"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..472
                     /gene="rpmA"
                     /locus_tag="AC16FU_03530"
     CDS             206..472
                     /gene="rpmA"
                     /locus_tag="AC16FU_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009870504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR2993479.1"
                     /translation="MAHKKGVGASRNGRDSHGQRLGVKAYGGEFVPAGSIIVRQRGTR
                     IYPGLNVGMGKDNTLFARITGTVHFETKGGDKKMVRVEPLPQTA"
     gene            638..1303
                     /locus_tag="AC16FU_03535"
     CDS             638..1303
                     /locus_tag="AC16FU_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993480.1"
                     /translation="MKHPVGIILGGCGVTEGSDPYEVAFCAYHIERHGWAALFFAPPE
                     ASSTEKKNPAVWSAAQQLSRGELLALDAPPSENLTALVIPGGEGILGTLATTFHDGNE
                     IDIEPNLKRLIRDLHRRKRPIAACGVAGALVVASLFHPESLSPTVAVGKDTRWIGLVE
                     TLGGVPVQVRSDEVVIDEKNRIVTTPGFAADEQLQMVERGIKNLIDSVGQWMPKGQPM
                     VAL"
     gene            complement(1326..2582)
                     /locus_tag="AC16FU_03540"
     CDS             complement(1326..2582)
                     /locus_tag="AC16FU_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR2993481.1"
                     /translation="MRDESPPRRSFFREALQNWKIVGYLGPAFLVSVGYMDPGNWGTD
                     IQAGSDFNYNLLWVLLLSNLMAILLQTLAAKLGLVTGRTLAQNCRDHYPRWLNIFLWV
                     LTEAAMMATDLAEFLGAAIGFYILFRIPMFSAGLLTGGVVFLILALYRFGYRKVEFAI
                     IGLVAVIGFCYVIELFLAKPNLGQLAYHTVVPWINDKSILVAVGMLGATVMPHNLFLH
                     SGVIQYRLQNGANSLKKKLFRFAVIDSMLALNLAFLVNAAILIMSAAVFHRNGVPVSS
                     IQEAHRTLIPLLGGFSSVAFAVALLSSGLSSSTTGTLAGQIVIEGFLNVKMGLWLRRL
                     ITMIPALVVIALGVNELKVLVLSQVTLSLALPFATIPLIWFTTRKKLMGEFANKPVTN
                     VLAIGAASIIIFLNGLLLYQVLGGSF"
BASE COUNT          592 a          679 c          815 g          501 t
ORIGIN      
        1 aaaggtgggg cgacccagtg ggttaccccg gcgatttttt gttgaccccc tgacccttcg
       61 actctgctca gggctgtccc ccttatcaag ggggacagaa gggagtgata agtacgactt
      121 ggcggtcaat cctgcggtgg ccagcggcgg cttgtgttcg cgaggagagt ttcgtatact
      181 actccagatt taaggaggca attcgatggc gcataagaaa ggtgttggtg cttcgcggaa
      241 cggcagagat tcgcacgggc aaaggctcgg cgtcaaggcc tacggcgggg agtttgtccc
      301 ggcagggtcg attatcgtcc gccagcgcgg gacgcggatt taccccggac tgaatgtcgg
      361 catggggaag gacaacacac tttttgcccg gataaccggc accgtccatt tcgagaccaa
      421 aggcggggat aagaaaatgg tccgcgtgga gccgctgccg caaacagcgt agggtgtagg
      481 ggtttgatta atcaaacccc tacaggtgta tccattgaaa aggatgggca gacacagggg
      541 tctgctccta cgatgaggat gtattaaggg cgggcgttcc ccgattggaa aatcggggtt
      601 tttcttttgg gtctccctgc ttatgtttcg ttgaaccatg aaacatccgg taggcatcat
      661 tctgggcggt tgcggggtca ccgaagggag cgacccgtac gaggtcgcct tttgcgccta
      721 tcacatcgag cggcacggct gggcggcact tttttttgct cctccagaag cgtcttcaac
      781 agaaaagaaa aatccagcgg tgtggtcggc ggcccagcag ctttcccgcg gtgaactctt
      841 ggctctcgat gcacctccca gtgagaacct gaccgctctg gtcattcccg gcggcgaagg
      901 gattttagga acgctggcga caaccttcca tgacggtaat gaaattgaca ttgaaccgaa
      961 tttaaagcgg ctcatccgcg acctccaccg gcggaagcgg ccgattgccg cttgcggcgt
     1021 ggccggggcg ctcgtcgtcg cgtccctttt ccatcccgaa tcactctccc cgacggtggc
     1081 ggtgggcaag gacacccgct ggatcgggct ggtggaaact ctgggcggcg tgccggtgca
     1141 ggtgcggtcg gacgaggtgg tgatcgacga aaagaaccgc atcgtcacca cgcccggctt
     1201 tgcggcggat gaacaattgc aaatggtgga acgggggatt aagaacctca ttgattcagt
     1261 cgggcagtgg atgccgaagg ggcaacctat ggtcgcgtta tgaaagaaca ggaactgccg
     1321 ccggattaga acgacccgcc cagaacctgg tagagcagaa gtccgttcag aaagatgata
     1381 atgctggcgg cgccgatagc caggacgttg gtgaccggct tgttggcgaa ctcgcccatc
     1441 agttttttcc gggtggtgaa ccagattagg ggaatcgtgg cgaagggaag cgccaagctc
     1501 aaggtcacct gactcaaaac cagcaccttc agttcgttta cgcccaaagc gatcacgacg
     1561 agagccggaa tcatcgtgat caggcgcctg agccagaggc ccattttgac gttcaaaaag
     1621 ccctcgatca cgatttgacc ggcgagagtg ccggtggttg atgaagacag tcccgaggag
     1681 agcagggcga cggcaaaggc aacgctggaa aatccgccca ggagcggaat gagtgtgcgg
     1741 tgcgcttctt ggatcgatga caccggcacg ccattcctgt ggaaaaccgc cgctgacata
     1801 atgagaatag cggcattcac gagaaaagcc agattgagtg ccagcatcga atcaatcacc
     1861 gcgaaacgga acagtttctt cttgagcgag ttggcgccgt tctgcaggcg gtactgtatg
     1921 acgccggagt gcaagaagag gttgtggggc atcaccgtgg cgcccagcat gccgacggcg
     1981 accaggatgc tcttgtcgtt gatccacggg accaccgtgt ggtaggcaag ctgaccgagg
     2041 ttgggcttgg ctaagaagag ttcaatgacg tagcagaacc cgatcaccgc caccaggccg
     2101 atgatggcga attccacctt gcggtagccg aagcggtaga gcgccagaat gaggaaaacc
     2161 accccgccgg tcaaaagccc tgcggagaac atcggaattc taaacaggat gtaaaagccg
     2221 atagcagcgc ccaaaaattc cgctaagtcg gtggccatca tagcagcttc ggtcaatacc
     2281 cagagaaaga tattgagcca gcgcgggtag tggtcgcggc agttttgcgc taatgttctt
     2341 ccggtgacca agccgagttt ggcggcgagc gtctgcagga gaatggccat caggttggag
     2401 agcaggagca cccagaggag gttgtagtta aaatccgagc cggcctggat gtcggtaccc
     2461 cagttgccgg ggtccatata ccccaccgac accagaaagg cgggccctaa gtacccgaca
     2521 atcttccagt tctggagcgc ttcccggaag aagctgcgcc ggggcggcga ctcgtcgcga
     2581 atccggc
//
LOCUS       DBNGZA010000170         4493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1942593, whole genome shotgun sequence.
ACCESSION   DBNGZA010000170 DBNGZA010000000
VERSION     DBNGZA010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1942593"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16FU_03545"
     CDS             complement(<1..303)
                     /locus_tag="AC16FU_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2993482.1"
                     /translation="MGKPITTNVMEERTDVARPPVRYRRMERPLWRDYAEASVIALVI
                     VIGPEKNLSFLMRSFAGTAKAYDHIGLLLVGDGPERQDLQEQAAYLKIDDRVHFAGS"
     gene            complement(447..1883)
                     /locus_tag="AC16FU_03550"
     CDS             complement(447..1883)
                     /locus_tag="AC16FU_03550"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR2993483.1"
                     /translation="MKTRIEKDALGSLAVPANAYYGIFTVRASKHFHLTGTKTHPELI
                     KAYALIKLACAKANVALGQLDRKRGQAIIRAAEEVLAGKFNDQFKIDMLQAGAGTPMH
                     MNANEVIANRAIELLGGKKGDYSLVNPNNQVNFGQSSNDVTPTAIRLAARARTEPLLE
                     ELALLAGAFAAKAKEFDRIVKVGRTHLQDAVPIRLGQEFSAYREAVARGRERIAHAIL
                     ELHELQLGGTAVGTGITAHPRMAPLAIREIARRTGFPCYPAKNRFYLMQSMAAFAVVA
                     SALEIFALDMLKVADDLMLLSSGPRAGLGEILLPSIEPGSSIMPGKINPSVVEAFKMV
                     CLQVIGNSRAIHAAVPEGDLEINVMTPLIAYNLIGALEVLTNGCRLFRRHCVAGIKAD
                     RIRTKELFDRAIATATALSPYISYQTTADLVHAALAKNRPLEDLVVERGVLPRDVVRK
                     LLRPEAQTEPGGIDKALLRRIRAAKKPS"
     gene            complement(2116..2595)
                     /locus_tag="AC16FU_03555"
     CDS             complement(2116..2595)
                     /locus_tag="AC16FU_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR2993484.1"
                     /translation="MLSKRHLKSVWHALDHAIIRFLSMYSLLLLRVALAATFIWFGAL
                     KIFGVSPVADLVAQTVYWVSPKFFVSFLGVWEVVIGLGLLFAVALRFVLFLFVLQMAG
                     TFLVLVLRPEVAFQSGNPLLLTVTGEFVIKNLVLIAAGMVIGTTVRRNQKIILNGEA"
     gene            complement(2730..>4493)
                     /gene="lepA"
                     /locus_tag="AC16FU_03560"
     CDS             complement(2730..>4493)
                     /gene="lepA"
                     /locus_tag="AC16FU_03560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR2993485.1"
                     /translation="AHIDHGKSTLADRLLEATGTLPPREMREQVLDNMDLERERGITI
                     KAHAVRMSYRAKNGQTYELNLIDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQT
                     ISNLYLALENNLTIIPVINKIDLPAAQIPETKKQFADLLGAGDEEIILASAKEGRGIA
                     EILEAIIARIPPPADTPDAPLRALIFDSTFDPYRGAVAYVRVVDGRIAKGTKIKFFAH
                     ERIYEVDEVGYLRLRQVPLDSLNPGEVGYVIANIRNVSDTRVGDTITEALRPAPAPLP
                     GFKHIKPMVFSGLYPSAAENYEQLRDAIEKLKLNDASLLYEPEMSNALGFGFRCGFLG
                     LLHMEIVQERLSREYGLSIIATVPNVEYRVTKTSGEVILVDNPAFLPPPGNLEKIEEP
                     FVVVQIITPAEFIGNVMKLCTERRGNYLTTEYVDKSRASLKYEMPLSEIIFDFYDRLK
                     STTNGYASLDYEYLDYREGPLVKLDILINGDSVDALSVIAHRDKAYHWGKQLCEKLRQ
                     LIPRQMFEVAIQAAIGARVIARETVKALRKNVTAKCYGGDITRKRKLLEKQREGKKRM
                     RQIGQVEIPQEAFLAVLKVER"
BASE COUNT          928 a         1323 c         1308 g          934 t
ORIGIN      
        1 cgaaccagcg aaatgcaccc ggtcatcaat tttaagatac gcagcttgtt cctggagatc
       61 ttggcgttct gggccgtctc ccaccagtag aagaccgata tgatcgtagg ccttggcagt
      121 tccagcaaaa gatctcatta aaaagcttaa gtttttctcc ggtccaatga cgataaccag
      181 cgcaatcacc gacgcttcgg cgtagtcccg ccagagcggc cgctccatcc ggcggtagcg
      241 caccggcgga cgggcaacat ctgtccgttc ttccattacg ttagtagtta tcggtttacc
      301 catagaaaag tttatccggc gatcggcggc tgcaagacca attcggatca gcagggtaac
      361 aacacaggag aaatttaaca gcctgcggag acagccccca cttgcaaaaa cccactcgct
      421 cttaacgagt cagaagtatt ggctggtcag gatggttttt ttgcggctcg aatccgccgc
      481 aaaagagctt tatcaatccc acccggctcg gtttgcgcct cgggccgcag gagtttccgc
      541 accacgtccc tgggcaaaac gccccgctcc accaccaggt cttccagcgg gcggtttttc
      601 gccagcgccg cgtgcaccag atcggcggtc gtctgatagg aaatgtaggg agaaagcgcg
      661 gtagcggtgg ctatcgcccg atcaaacaac tctttagtcc gtatccggtc ggccttaatt
      721 ccggccacgc aatgccggcg gaagaggcgg cagccgttgg tcaggacttc gagagcgccg
      781 atgaggttgt aagcgatcag gggcgtcatc acgttgattt ccagatcccc ctccggcacg
      841 gcggcgtgga tcgcccgcga attgccgatc acctgcaggc agaccatctt gaacgcttcc
      901 accaccgagg ggttgatctt ccccggcata atggaggagc ccggctcgat ggaaggcaat
      961 agaatttctc ccagcccggc gcgcggcccg gaggagagga gcatcaggtc gtcggccact
     1021 ttcagcatat cgagggcgaa aatctccagc gctgaggcga ccacggcaaa agccgccatc
     1081 gactgcatca ggtaaaagcg gtttttggcc gggtagcagg ggaagccggt gcggcgggcg
     1141 atttcgcgga tcgccagagg cgccatgcgc gggtgggcgg tgatgccggt gccgaccgcc
     1201 gtcccgccca gttgcagctc gtgcaattcc agaatggcgt gggcaatacg ctcccgtcca
     1261 cgggccaccg cttcccggta agcggagaac tcctgcccca accggatggg gaccgcgtcc
     1321 tgcaggtggg tccgccccac tttcactatg cggtcgaatt ctttcgcctt agcagcaaag
     1381 gctcccgcca ggagggcaag ttcttccaac aacggctccg tacgcgcccg ggcggccagc
     1441 cggatggccg taggcgtcac gtcgttggac gactggccga aattgacctg attgttcgga
     1501 ttcaccagcg agtaatcacc ctttttgccg cccaaaagtt caatggcccg gttggcgatt
     1561 acctcattgg cgttcatatg catgggagtg ccggccccgg cctggagcat gtcgattttg
     1621 aactggtcgt tgaacttccc cgccagcact tcctcagctg cccggatgat cgcctgcccg
     1681 cgcttgcggt ccaactgtcc caaggccacg ttcgccttgg cgcaggcgag tttaatcaag
     1741 gcgtacgctt tgatcagttc cgggtgggtt ttcgttccgg tcaggtggaa atgttttgac
     1801 gcccggacgg taaaaatgcc gtagtaggca ttggccggaa cggctaggct ccccagggca
     1861 tctttttcga ttcgtgtttt catctaagtg ctcctctatg attcccgctt ttggccagca
     1921 tggagaagaa caaatgcgtc tgatgggaaa ttggcaaggc ggggttgggg atttaggagt
     1981 tgggatatag gatttcggga aaacaccggg aggggcgact ttagtggttc aacccgccta
     2041 tggcgggttc accacggtga gtccgccata ggcggatcga accgtcgccc ggcacacttt
     2101 tacgctcgac tgaagtcaag cctccccgtt gagaataatc ttttgattcc gacgcaccgt
     2161 cgtaccaatc accatcccgg cggcgatcag caccagattt ttgatgacaa attcccccgt
     2221 cacagtcaaa agcagtgggt tgccggattg gaaagcaacc tcgggccgca gcaccagcac
     2281 gagaaatgta cccgccatct gcaagacaaa caaaaacagg acaaaacgta acgccacggc
     2341 aaacagaagc cccagcccga tcaccacttc ccagacgccg aggaaagaga caaaaaactt
     2401 cggcgacacc caataaactg tttgcgcgac cagatcggca accggactta cgccaaagat
     2461 tttgagtgcg ccgaaccaga tgaaggtggc cgccagcgcc acccgcaaaa gcaacaggct
     2521 gtacatagag agaaatcgaa tgatggcgtg gtcaagagcg tgccagaccg atttcaaatg
     2581 tcttttggag agcaaagtga tactcaatcc ggtcaatgtc cccgcattgg tggtactttc
     2641 aactgcccaa aacacgggag gggcgacttc agtcgcccac taaactttta attgctcggc
     2701 tgaaatcgag cctcccagtt acatagatct caccgttcta ccttcaacac cgctaagaac
     2761 gcctcctgcg gaatctccac ctggccgatc tggcgcatcc gcttcttccc ctcgcgctgt
     2821 ttctccagca gtttccgctt gcgggtgatg tcgccgccgt agcatttggc ggtgacgttc
     2881 ttgcgcaggg ccttcaccgt ctcccgggcg atcacccgcg cgccgatggc ggcctgaatc
     2941 gccacctcga acatctggcg cgggatcaac tgccggagtt tttcgcacag ctgtttcccc
     3001 cagtggtagg ccttgtcccg gtgcgcgatc accgagaggg cgtccaccga gtcgccgttg
     3061 atcaggatgt ccaatttcac cagcggccct tcccggtagt cgagatattc atagtcgagg
     3121 gaggcgtacc cgttggtggt ggacttgagc cggtcgtaga agtcgaaaat gatctcagag
     3181 agcggcatct catacttcaa actggcgcgg cttttgtcca cgtactcagt ggtgaggtag
     3241 ttcccccgcc tctccgtgca gagtttcatc acgttcccga tgaactccgc cggcgtaata
     3301 atttgcacca ccacgaacgg ctcctcgatt ttttcgaggt tgcccggcgg cggcagaaaa
     3361 gcggggttat cgaccaggat cacctcgccg gaggttttcg tcacccggta ctcgacgttg
     3421 ggcaccgtgg cgatgatgga gagcccgtac tcgcgggaga gccgctcctg gacaatttcc
     3481 atgtgcaaga gtcccaaaaa accgcagcgg aaaccgaacc caagcgcgtt ggacatttcc
     3541 ggctcataga gcaacgaagc gtcgtttaat ttcagttttt caatggcgtc gcgcagctgt
     3601 tcgtagtttt cggcggcgga agggtacaag ccggagaaga ccatcggctt gatgtgcttg
     3661 aacccgggca aaggcgccgg cgccgggcgc agggcttccg tgatggtgtc gcccacccgc
     3721 gtgtcggaga cgttgcggat gttggcaatg acgtaaccga cttcgccggg gttcagggaa
     3781 tccaggggca cctgacgcag gcgcagatag cccacctcat ctacttcgta aatccgctca
     3841 tgggcgaaga atttgatttt tgtgcctttg gcaatccgcc catccaccac ccgcacgtag
     3901 gccactgctc cgcgatacgg gtcgaaggtc gagtcgaaaa tcaaggcccg cagcggcgcg
     3961 tcgggtgtat ccgccggcgg cgggatgcgg gcgatgatgg cttcgaggat ttcggcaatg
     4021 ccgcgtccct ctttggcgga ggcgagaatg atttcttcgt ctccggcgcc caagaggtcg
     4081 gcaaactgct ttttggtttc cggaatctgc gccgccggca ggtcgatctt gttgatcacc
     4141 ggaatgatgg tgaggttgtt ctccagcgcc aggtagaggt tggaaatggt ctgcgcctcc
     4201 accccctggg aagcatcgac caccaagagc gccccctcgc aggcggcgag ggaccgcgag
     4261 acttcgtagg tgaaatcgac gtgccccggg gtgtcgatca agttcagttc atacgtctgg
     4321 ccgtttttcg cccggtagga cattcgcacg gcgtgggctt tgatggtgat cccccgctcg
     4381 cgctccaggt ccatattatc cagcacctgc tcgcgcattt cccgcggcgg cagggtgccg
     4441 gtggcttcca ggagccggtc ggccagggtg gatttgccgt ggtcgatatg ggc
//
LOCUS       DBNGZA010000171         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2681269, whole genome shotgun sequence.
ACCESSION   DBNGZA010000171 DBNGZA010000000
VERSION     DBNGZA010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2681269"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="meaB"
                     /locus_tag="AC16FU_03565"
     CDS             <1..948
                     /gene="meaB"
                     /locus_tag="AC16FU_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011013089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR2993486.1"
                     /translation="VNTLERFRQKDPLALSKVISHVENRLSDYQKLLATLYPQTGRAY
                     RIGLTGPPGAGKSTLADKLTLLLVNEKKSVGIIAVDPTSPFTGGALLGDRIRMQDLTE
                     KKEVFIRSMATRGSVGGLAETTRDVALVLDAFGKEFIFIETVGVGQVELDIAEACDTT
                     LVILVPESGDAIQAMKAGLMEIADIFVINKADRPGAARIISELEMILDTRRKMEGWQY
                     PIVATEAINSKGIELLWQKISEHRQVISANGALEAKRRHQVKVQIEKLMASKVQEEVE
                     GKILHGVNLDEVAGRVFRHEVDPYSVCDELWRKRSRLSQ"
     gene            1085..>1720
                     /locus_tag="AC16FU_03570"
     CDS             1085..>1720
                     /locus_tag="AC16FU_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR2993487.1"
                     /translation="MVEALAPRTAEPRAVQLGLFSEETIAQVREGKQRWQEKVSKPLT
                     PEQRKQTLPKFITVSGEEIAPLYSPEEIQQINFLRDVGFPGEYPYTRGIHADMYRRRL
                     WTMRQFSGFGTPKQTNERYKFLLAQGQTGLSVAFDLPTLMGYDSDHPRSLGEVGVCGV
                     AVDSLKDMEIIFDGIDLGNISTSMTINSPASILLANYIVVAEKKGVPSHRLR"
BASE COUNT          399 a          497 c          497 g          327 t
ORIGIN      
        1 gtgaacactc tcgaacgctt ccgccagaaa gatccgctgg ccctttccaa agtcatttcc
       61 catgtcgaaa accgcctgtc ggactaccaa aaattgctgg cgacactcta tccccaaacc
      121 ggccgggcct accggatcgg cctcaccggc ccgcccgggg ccgggaagag cacgctggct
      181 gacaaactca ccctccttct ggtcaacgaa aaaaaatcgg tggggatcat tgccgtcgat
      241 cccacctcgc ctttcaccgg cggcgcgctc ttgggcgacc ggatccggat gcaggatttg
      301 accgaaaaaa aagaggtctt tatccgcagc atggccaccc gcggctcggt ggggggcctg
      361 gccgaaacca ctcgcgacgt ggccttggtt ttggacgcct tcggcaagga gttcattttc
      421 atagaaacgg ttggcgtggg ccaggtggaa ctcgacatcg cggaagcctg cgatacgacg
      481 ctcgtgattt tagtccccga atcgggcgat gccattcagg cgatgaaggc gggcttgatg
      541 gagattgccg acatcttcgt catcaacaag gccgaccgcc ccggcgcggc gcgaattatt
      601 tctgaattgg agatgattct ggacacccgc cgcaaaatgg aggggtggca gtacccgatt
      661 gtggccacgg aagcgatcaa tagtaaaggc atcgaactct tgtggcagaa aattagcgag
      721 caccggcagg tgatttccgc gaacggtgcg ctggaggcca aaaggcgcca ccaggttaaa
      781 gtgcagattg aaaagcttat ggcgagtaaa gttcaagagg aagtcgaagg gaagattttg
      841 cacggcgtga acctcgatga ggtggcgggg cgggtttttc gccacgaagt cgatccgtac
      901 tcggtctgcg acgaactctg gcggaagcgg agccggttgt cgcagtgatg cgggttgcta
      961 tattaatgat gccatgcgca taatgcagaa agtgcagtcc cgcccggtga aaaagaaatc
     1021 aaggatacca tcagtctcga agagtcgagt ctccgacaaa aaaatgaagg gaaatatgaa
     1081 ccgaatggtc gaagcccttg caccccggac ggctgaaccc cgcgctgttc agctggggct
     1141 tttttccgaa gagacaatcg cccaggtccg cgagggcaag cagcgctggc aagaaaaagt
     1201 ttccaaaccg ctgaccccgg agcaacgcaa gcaaaccctg ccgaagttca tcaccgtctc
     1261 cggcgaggaa atcgctccgc tctattctcc cgaagagatc cagcagataa atttcctgcg
     1321 cgacgtcggc tttcccggcg agtaccccta cacccggggc atccacgccg atatgtatcg
     1381 gcgcaggctc tggacgatgc gccagttctc cggcttcggc acgcccaagc agactaacga
     1441 gcgctacaag ttcctcctcg cccagggcca gacgggtctt tcggtcgctt tcgatctgcc
     1501 caccctgatg ggatacgact ccgaccatcc ccgctcgctg ggagaagtgg gcgtctgcgg
     1561 cgtggcggtc gactcgctca aagacatgga aatcattttc gacggcatcg acctagggaa
     1621 tatctcaacc tcgatgacga tcaactcacc ggcttcgatt cttctggcaa actacatcgt
     1681 cgtggccgag aagaagggag tgccctcgca ccgcctgcgg
//
LOCUS       DBNGZA010000172         1015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2788997, whole genome shotgun sequence.
ACCESSION   DBNGZA010000172 DBNGZA010000000
VERSION     DBNGZA010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2788997"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16FU_03575"
     CDS             <1..198
                     /locus_tag="AC16FU_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HIR2993488.1"
                     /translation="AHNQQFLAALEENVAVGRRLNFLLQEMNREANTIGSKSLSAEIV
                     QRVVVLKEEIEKLREQVQNIQ"
     gene            361..942
                     /gene="gmk"
                     /locus_tag="AC16FU_03580"
     CDS             361..942
                     /gene="gmk"
                     /locus_tag="AC16FU_03580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR2993489.1"
                     /translation="MFIVLSSPSGGGKTTICRKLLARHRDFGFSVSATTRSKRPGERR
                     GRDYHFMSAEEFERKRKQGAFVEWARVYGACYGTLRREVANLARRFPVVLFDVDTRGG
                     LAIKRQYPHTALVFLLPPSSAAQRRRLARRGTDDRATQKARLAASQRELARMTRYDYV
                     VTNERLGQTIRAIESIITAERSKSTCTGTTRTR"
BASE COUNT          237 a          266 c          317 g          195 t
ORIGIN      
        1 gcgcacaacc agcagtttct ggcggcgctg gaggaaaatg ttgcggtggg aaggcggctc
       61 aatttcctcc tgcaggaaat gaaccgtgag gcgaacacca tcggttccaa atcactttcg
      121 gcggagattg tccagcgggt ggtagtgctc aaagaagaaa tcgaaaaatt gcgcgagcag
      181 gtacaaaata tacagtagcc agtggccagt agccagagaa aaccgaaagt ctccaacact
      241 tccgagttta agattagaga agtgggcgga gccgattcgt ttcgatctcc tcagggtgtt
      301 cactgaaaag cacaagctga tttctggagc cgcacgcaga gggtgaagcg ctccccgcgt
      361 ttgtttatcg tcctctcgtc gccctccggc ggcggcaaga ccaccatctg ccggaaactt
      421 ctggcgcggc accgggactt cggtttttca gtttcggcga ctacgcggtc caaacggccc
      481 ggcgagcgcc ggggtcgcga ttaccacttc atgtcggcgg aggaatttga gcggaaaaga
      541 aagcaggggg cgttcgtgga atgggcacgg gtctacggcg cttgctacgg cacgctgcgg
      601 cgcgaggtag cgaacctggc gcggcggttt ccggttgtac tctttgacgt tgatacacgc
      661 ggcgggctgg cgattaagag gcagtacccg cacacggcat tagtcttcct tttgccgcca
      721 tcttcggccg cccagcgccg gcgtctggca cggcgcggca ccgatgatcg cgccacgcaa
      781 aaagcgcgtt tggccgcctc gcagagggaa ctggcgcgga tgacgaggta cgattacgtg
      841 gtgacgaacg aaaggctcgg tcaaacaatt cgcgcgattg aaagtataat tacggcggag
      901 aggtcgaaat cgacttgtac cgggacgacg cggactcggt aacaatcccg aaactctcaa
      961 tagtaaggag aacagagcgt atgccgaaac tcgtaccatt ggacgatctg gaata
//
LOCUS       DBNGZA010000173         1102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1833492, whole genome shotgun sequence.
ACCESSION   DBNGZA010000173 DBNGZA010000000
VERSION     DBNGZA010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1833492"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="AC16FU_03585"
     CDS             complement(<1..844)
                     /locus_tag="AC16FU_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2993490.1"
                     /translation="MRTIESRPKILVVEDRGPDREMLTTVLQHAQYRVLEAADGPQAL
                     ALVRSEHPDLVISDLLLPGMDGYQLVRELRADPSISETGVIFYSAVFDEQEAQELAQE
                     CGVSRVLIKPMDPAKILENVAEVLKNRGVQKIQPLPASFEQKHQQLLVDKLVDQVKAL
                     QRSEERLRVKTQILTGINRVFHEALTTETEEELARTSLEVAKGLTGSRFALMGKVNQA
                     RKLDIALSDPEWGACPVSGSAQEDLSDSLPHLRGFFEKIIIEGRSLIANDLGGHPGST
                     TGPPD"
BASE COUNT          223 a          328 c          300 g          251 t
ORIGIN      
        1 agtccggggg cccggtggtg ctgccgggat ggcctcccag gtcattggcg atcagggacc
       61 gtccttcgat gatgattttt tcgaaaaagc cgcggagatg cggcaagctg tcggacaaat
      121 cttcttgggc cgatcccgac accgggcagg cgccccactc cgggtcactg agggcaatat
      181 ccaacttgcg agcctggttc accttcccca ttaaggcaaa cctgcttccc gtcaagcctt
      241 tggccacctc cagactggtg cgggccaatt cttcttcagt ctctgtggtc aaggcctcgt
      301 gaaagacccg gttgatcccg gtcaggattt gagtcttcac ccggaggcgc tcctcgctcc
      361 gttgcagcgc cttgacctgg tctacgagct tgtctaccaa cagttgttgg tgcttctgct
      421 caaacgaagc cggcagaggt tggatcttct ggacgcccct gtttttcaga acctcggcca
      481 cgttctccag gattttcgcc gggtccatcg gtttgatgag cacccggctg accccgcatt
      541 cctgggccag ttcctgggct tcttgctcat caaataccgc ggaatagaaa atgacgccgg
      601 tctcggagat cgagggatcg gcgcggagct cgcggaccaa ctgatagccg tccattccgg
      661 gaaggagcag gtcggaaatg accaggtcgg gatgttcgga gcgtaccagc gccaaagcct
      721 gggggccatc ggccgcctcc agcacccggt actgggcatg ttgcagcacg gtggtcagca
      781 tttcccggtc aggtccacga tcctccacga ccagaatttt gggccggctt tcaatagttc
      841 tcatgtcaaa attgctccaa attactcgac tcagtgcata atctcataaa taccctaaag
      901 tatcatatct ccctgcgtcc tctgcggtga aatttttacc gcagagacgc agagagcgcc
      961 gagaaaaaac ttttgcaaaa ggctcctggg caagtcattt ccccatgaac gattccacct
     1021 ggtttttaaa ggtctcaggt gcaatcggtt tgtcaatgta gccgtcaaat ccggcggcga
     1081 gcacccgctc gcgatcgccg ac
//
LOCUS       DBNGZA010000174         6508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_773777, whole genome shotgun sequence.
ACCESSION   DBNGZA010000174 DBNGZA010000000
VERSION     DBNGZA010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_773777"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..364
                     /locus_tag="AC16FU_03590"
     tRNA            292..364
                     /locus_tag="AC16FU_03590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:325..327,aa:Thr,seq:ggt)
     gene            434..1639
                     /gene="tuf"
                     /locus_tag="AC16FU_03595"
     CDS             434..1639
                     /gene="tuf"
                     /locus_tag="AC16FU_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR2993491.1"
                     /translation="MAKPKYERTKPHVNVGTIGHVDHGKTTLTAAITMVLNRKGLAQV
                     RTFDSIDNAPEERERGITIATAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKVDAVDDPELLDLVELEVRDLLKK
                     YEFPGDKLPVIRGSALQAMTQAIKPEVKDDPAFKCVYELMAAVDSYIPTPKREIDKPF
                     LMPVEDVFSITGRGTVGTGRVERGKVKVSDELERVGFRETKKTVVTGVEMFRKTMDYA
                     EAGDNVGLLLRGIEKEDLERGMVLAKPGSITPHTKFKGEVYVLTKEEGGRHTPFFTGY
                     RPQFYFRTTDVTGVIKLPEGVEMVMPGDNITMEVELITPIALEKELRFAVREGGHTVG
                     AGVVGTILE"
     gene            1694..1846
                     /gene="rpmG"
                     /locus_tag="AC16FU_03600"
     CDS             1694..1846
                     /gene="rpmG"
                     /locus_tag="AC16FU_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR2993492.1"
                     /translation="MAREIITLACGECKRRNYSGTKNKKKHPDRVEFKKFCPFCSRHT
                     PHKEVR"
     gene            1934..2007
                     /locus_tag="AC16FU_03605"
     tRNA            1934..2007
                     /locus_tag="AC16FU_03605"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1968..1970,aa:Trp,seq:cca)
     gene            2059..2253
                     /gene="secE"
                     /locus_tag="AC16FU_03610"
     CDS             2059..2253
                     /gene="secE"
                     /locus_tag="AC16FU_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR2993493.1"
                     /translation="MGGWIEKITRYLKEVRAELQKVSWPSRKELNASTVIVIFVSLVL
                     ALFIFGVDNVLSMVLSLVIK"
     gene            2253..2798
                     /gene="nusG"
                     /locus_tag="AC16FU_03615"
     CDS             2253..2798
                     /gene="nusG"
                     /locus_tag="AC16FU_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR2993494.1"
                     /translation="MAMRWYVAHTYAGHEQKAKKYLEHAATAAGLADRIGQILVPTES
                     VNEMKQGKRATSLRKFLPSYILIEMDLDRETQALVVNTPAITNFVGGGGKPVPLKPDE
                     VERILEQIETGSTREPKEIPYKIGDPIKVIDGPFSDFSGTVSEVNEEKKRVKVMVSIF
                     GRPTPVELDFLQIQKQEKKRR"
     gene            2803..3228
                     /gene="rplK"
                     /locus_tag="AC16FU_03620"
     CDS             2803..3228
                     /gene="rplK"
                     /locus_tag="AC16FU_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR2993495.1"
                     /translation="MAKKIVATVKLQISAGQANPAPPVGPALGQHGVNIMEFCKAFNA
                     RTQSQTGLIIPVIITIFADKSFTFITKTPPASVLLLKAAKVEKGSGEPHRVKVGSVTR
                     AQVREIATVKMPDLNAASLEAAERLVAGTARSMGIEIVG"
     gene            3232..3945
                     /gene="rplA"
                     /locus_tag="AC16FU_03625"
     CDS             3232..3945
                     /gene="rplA"
                     /locus_tag="AC16FU_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR2993496.1"
                     /translation="MPLKSGKKYRKATEGLEPARRYPLAAAVKEVKERRFAKFDEAVE
                     VSVRLGVDPKQADQIVRGTVVMPHGTGKKVRILVLAKGEKEKEAQAAGADYVGAEEYV
                     AKIQQGWTDVDAIIATPDLMGQVGKLGKILGPRGLMPNPKSGTVTFDVAKAVKDLKAG
                     KIEYRVDKQGNIGTAIGKTSFSEQQLTENAQAFLAAVVRARPASVKGQYLRNVTLSST
                     MGPGVKVDPQEVLNLAKGL"
     gene            3950..4513
                     /gene="rplJ"
                     /locus_tag="AC16FU_03630"
     CDS             3950..4513
                     /gene="rplJ"
                     /locus_tag="AC16FU_03630"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR2993497.1"
                     /translation="MKREAPATFPVDVHEPRPEKKAAVEQLAQKFRSSGSFFLTDFTG
                     LTVAQATTLRKNFREQKVEYVVAKNTLIRLAAHQTGLEGLAEHLSGPTGVVFGADDPV
                     VAAKILFEFIKKAEKPRLKAFWVTGQKYARADFNRLAQLPSRPELLARVVGVLNAPLA
                     NLVSTFDGILRTFIGTIEAIAEKKQKL"
     gene            4612..4992
                     /gene="rplL"
                     /locus_tag="AC16FU_03635"
     CDS             4612..4992
                     /gene="rplL"
                     /locus_tag="AC16FU_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR2993498.1"
                     /translation="MAEAKVAELVGQIESLTVLELAELSQTLQEKFGVSAAVPMAAPA
                     GGGAAPAAAIEEKTEFSVVLAGTGDKKIQVIKVIRELTGLGLKEAKDLVDGAPNVVKE
                     GIPKEEAETIRTKLQEAGATVEIK"
     gene            5193..>6508
                     /gene="rpoB"
                     /locus_tag="AC16FU_03640"
     CDS             5193..>6508
                     /gene="rpoB"
                     /locus_tag="AC16FU_03640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016450.7"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR2993499.1"
                     /translation="MAQATIERKSYGRIDTVVEMPNLLDVQLGSFNDFLQMDVPPAER
                     KHVGLQQIFQEIFPISDVHENYALEFVKYQVGTPRYSIAECQERNMTYAAPLKATLRL
                     MTKGGEGKEKRVRDIIEQDVFLGEIPLITEKGTFIVNGAERVIVSQLHRSPGVFFDEE
                     VHPNGKKLFSARIIPYRGSWVEFNVDVNDLLYCHIDSRRKLAGTTLLRALGYATDEAI
                     TSLFYETDEEDLSGRKGSKAVGKISAASVVDQATGEIILQAGDPITEEILEKIKQAKI
                     ESLRVIKSDLSRDAGVILNTLKKDAAHSQEEALLKIYALIRPGEPPTPEVAFGVLERL
                     FFNAKRYDLGKVGRYMIDERLNLDIPLDCTVLDKRDFIAIIKYLLDLRNGLGDVDDID
                     HLGNRRTRTVGELLSNQFSVGLSRMARTMRERMSLKDTDSITPHDLV"
BASE COUNT         1516 a         1822 c         2026 g         1144 t
ORIGIN      
        1 cgtctcccgg tttgaagtct ttggataagt aaagaagatg gagatagtcc tgcgccaaga
       61 gggtggttct ggaggcgcgg ccgcgaaaat ttaccggggc ccaaaaagga cttgacaaaa
      121 aaatgaaggg gtgtataata ataacttacg tgctctgcta acttgcagaa tgccggttgg
      181 tggggtatac ttgtttgttc ggctcagggg aaagagcata ttcacatcca gcatcaaatg
      241 ctccccaata agccggcaca acgggaagca tttgaaacgg tgacaaggac tgcccagata
      301 gctcagttgg tagagcacgt ccttggtaag gacgaggtca ccggttcaat cccggttctg
      361 ggctgaatta cggaaacgcg ccgaagacgg aaaacgaaag taaagggacg aagcagaggc
      421 tgagggagac ggtatggcca aaccaaagta cgaacgaacg aagccgcacg taaacgtcgg
      481 caccatcggg cacgtggacc acggcaagac cacgctcacc gccgccatca ccatggtgct
      541 gaaccgcaaa gggctggcac aggtccggac gtttgactcc atcgacaacg cgccggagga
      601 gcgggagcgg ggcatcacca ttgccacggc gcacgtggag tacgaaaccg acaaaaggca
      661 ttacgcccac gtcgactgcc ccggacacgc cgactacgtc aagaacatga tcaccggcgc
      721 agcccagatg gacggcgcga tcttagtcgt ttccgccgcc gacgggccga tgccccagac
      781 gcgcgaacac atccttttgg cccggcaggt gggcgtgccg tacatcgtgg tgttcctcaa
      841 taaagtggat gcggtggatg atccggaact tttggatctg gttgagctgg aagttcggga
      901 cctcctgaag aagtacgagt tccccggcga caagcttccg gtcatccggg ggtcggccct
      961 gcaggcgatg acccaggcca tcaagccgga ggtgaaagac gatcccgcgt tcaagtgcgt
     1021 ctacgaactg atggcggcgg tcgattccta catccccacc cccaagcgcg agatcgataa
     1081 gccgtttttg atgccggtgg aggacgtctt ctccatcacc gggcgcggca cggtgggcac
     1141 cggccgggtg gagcggggca aggtgaaagt gagcgacgaa ctggaacggg tcggtttccg
     1201 cgaaacgaag aaaaccgtgg tcaccggggt cgagatgttc cgcaagacca tggactacgc
     1261 cgaagcgggc gacaacgtcg gtttgctctt gcgcggcatt gagaaggaag acttagagcg
     1321 cggcatggtt ttggccaagc ccggttccat caccccgcat accaagttca aaggggaagt
     1381 ctacgtcctg accaaagaag aaggcggccg gcacacgccc tttttcaccg gttaccggcc
     1441 ccagttttac ttccgcacca ccgacgtgac cggcgtcatc aaactgcccg aaggggtgga
     1501 gatggtgatg cccggggata acatcacgat ggaagtggaa ctgatcaccc cgattgcgct
     1561 ggagaaggaa ctgcgctttg ccgttcgcga gggcggccac acggtgggcg ccggggtcgt
     1621 gggaacgatc ctggagtaag ggctctgcgg ggcgcaggcc cttgaatttt tgtctctgaa
     1681 tgcgtggtaa agtatggcgc gagaaatcat tactttggcg tgcggggaat gcaagcggcg
     1741 caactattcg ggcaccaaga ataaaaagaa gcatcctgac cgcgtggagt ttaaaaagtt
     1801 ttgcccattc tgcagccggc acacgccgca caaagaggtg cggtaatccc gcctgtcgga
     1861 gactcgtcgt agactcgact cttcgagact cttcgagagg cggcccccgg acgcaagcga
     1921 gtgacagatt cttaggactg tagctcaact ggccagagca ccggtctcca aaaccggggg
     1981 ttgggggttc gagtccctcc agtcctgttg ggtgtcccgc ccctggcggg attcgaaaga
     2041 tcggtcgaga ggcaacattt gggcggatgg attgaaaaga taacccggta cctgaaggag
     2101 gtccgggccg aactgcagaa ggtgagctgg ccgtcacgca aagagctgaa cgcctccacg
     2161 gtgatcgtca ttttcgtttc gctcgttctg gcgctcttca tcttcggcgt ggataacgtt
     2221 ctttctatgg ttttgagtct ggtgattaag tgatggcgat gcggtggtac gtggcccaca
     2281 cctacgccgg ccacgagcag aaggcaaaga agtacttaga gcatgcggcg acggcggccg
     2341 ggctagccga ccgcatcggt caaatcctgg tgccgaccga gtcggtgaat gagatgaaac
     2401 agggcaagcg ggccacgagc ctgcggaagt tcttgccctc ctacatcctg atcgagatgg
     2461 atctcgaccg cgagacccag gccctggtgg tcaacactcc ggccatcacc aacttcgtgg
     2521 gcggcggggg aaaaccggtg ccgctcaagc cggacgaggt ggagcgcatc ttagagcaga
     2581 tcgaaaccgg cagcacccgg gagccgaagg agatcccgta caagatcggc gacccgatca
     2641 aggtcatcga cggcccgttc tccgatttct ccggcacggt gagcgaagtg aacgaggaga
     2701 agaagcgcgt caaagtgatg gtctccatct tcggccggcc gacgccggtg gaactggatt
     2761 ttttgcaaat tcagaagcag gaaaagaaaa ggcggtaagg cagtggcgaa aaagatcgtc
     2821 gcaacggtga aactccagat ctccgccggg caggccaatc cggccccccc ggtgggcccg
     2881 gctttaggcc agcacggggt gaacatcatg gagttctgca aagccttcaa tgcccgcacg
     2941 cagagccaga cggggctcat cattccggtg atcatcacca ttttcgccga caagtcgttc
     3001 acctttatca ccaaaacgcc gccggcctcg gtcctcttgc tcaaagcggc caaagtcgaa
     3061 aaagggtccg gggagccgca ccgggtcaaa gtcggctcgg tcacccgggc gcaggtacgg
     3121 gagatcgcca ccgtgaaaat gccggatctg aatgcggcca gtctggaagc ggccgagcgg
     3181 ctcgtggccg gcacggcccg cagcatgggc atcgaaatcg tcgggtaagg catgcctttg
     3241 aaatccggta aaaaataccg caaagcgact gagggactgg aaccagcccg gcgctatcca
     3301 ttggccgccg ccgtgaaaga ggtcaaagag cgcaggttcg ccaagttcga tgaagcggtg
     3361 gaagtttcgg tgcggctggg ggtcgatccc aagcaggccg atcagattgt ccgggggacc
     3421 gtggtgatgc cccacggtac cggcaagaag gtgcgcattc tggttttggc caagggcgag
     3481 aaggagaagg aagcgcaggc ggccggcgcg gactacgtcg gcgccgagga gtatgtcgcc
     3541 aagatccagc agggctggac cgacgttgat gccattatcg ccacccccga tctgatgggc
     3601 caggtgggga aactcgggaa aattttaggc ccgcgcgggc tcatgcccaa tcccaagtcg
     3661 ggcacggtca ccttcgacgt ggccaaggcg gtgaaagatt tgaaagccgg aaaaatcgag
     3721 taccgcgtgg acaagcaggg gaacatcggc acggccatcg ggaaaacgtc gttttccgaa
     3781 cagcagttga ctgagaacgc ccaggccttc ctggcagctg tggtgcgggc ccggccggcg
     3841 tcggtcaaag gccagtactt aagaaacgtc acgctgtctt ccactatggg cccgggggtg
     3901 aaagtagacc ctcaggaggt cctgaactta gccaagggat tgtgagacta tgaagcgaga
     3961 agcaccggcg acttttcccg ttgatgttca cgaaccccga ccggaaaaaa aggcggcggt
     4021 ggaacaattg gcgcagaaat tccgctccag cggttcgttc tttttgaccg acttcaccgg
     4081 tttgacggtg gcccaggcga cgacgttgcg caagaacttc cgggagcaga aggttgaata
     4141 cgtcgtggcc aaaaatacgc tgatccggct ggccgctcac cagaccggct tggagggctt
     4201 ggccgaacac ctgtccgggc cgactggggt cgttttcggt gccgacgacc cggtggtggc
     4261 cgccaaaatc ctcttcgaat tcatcaagaa ggcggaaaag ccgcgcttga aggctttctg
     4321 ggtgaccggt caaaaatatg cccgggcaga tttcaatcgc ttagcgcagc tgccgagccg
     4381 cccggaactt ttagcgcggg tggtcggggt cttgaacgcc ccactggcaa atctcgtcag
     4441 cacgttcgac ggtatcctgc ggacctttat cggaaccatt gaggcgattg ccgaaaaaaa
     4501 acagaagctg taagtcgagg ccagcaacgg aactcactcc tgcaggttcg ctgccgggcc
     4561 gaaagcgaga aataacccga taaaaagaga aaaacgagaa gaggagagac gatggcagaa
     4621 gcgaaagtag ctgaactggt cggtcagatc gagagcctga ccgttctgga attggcggag
     4681 ctctcgcaga cgctccagga aaagttcggc gtttccgccg ccgtgcccat ggcggcgccc
     4741 gcaggcggcg gtgcggcgcc ggccgcggcg atcgaggaga aaacggagtt ttccgtggtt
     4801 ctggccggaa ccggcgacaa gaaaattcag gtgattaagg tgatccggga actgaccggc
     4861 ctgggtctca aggaagccaa agatctggtg gacggagcgc cgaacgtggt caaggaagga
     4921 attcccaagg aagaagcgga aacgatccgg accaagctgc aagaggccgg agccacggtt
     4981 gagataaagt aagaattgcc gaagaatccc cttaggaacg gtccgggtgc ccgtacgggc
     5041 cgcggacgga gccgaacggg aatcggtgtc tccgctgcta ttcgtgttca gaaacgaaca
     5101 gtaccacttt cgtataagcc agggcgggaa aactccggcg cacccggggg aaaagcccgt
     5161 ctatccctat tatccgaaga ggagtgaggc ctgtggcgca agcaaccatt gagcgcaagt
     5221 cgtacgggcg gatcgatacc gtcgtcgaga tgccgaacct cctggacgtg cagctgggtt
     5281 cgttcaacga ttttctccag atggacgtgc cgccggcgga gcgcaagcac gtcggcctgc
     5341 agcagatttt ccaggaaatc ttccccattt ccgacgtgca cgagaactac gctttagaat
     5401 tcgtcaagta ccaggtgggc acgccgcgct actccatcgc cgagtgccag gagcggaaca
     5461 tgacttatgc ggccccgctc aaggccactc tccggcttat gaccaaaggc ggtgagggga
     5521 aggagaagcg ggtccgggac atcatcgagc aggatgtctt tctgggcgag atccccctca
     5581 tcaccgagaa gggaaccttc atcgtcaacg gggccgagcg ggtcatcgtc tcgcagctcc
     5641 accgctcgcc cggagttttc ttcgacgagg aagtccaccc caacgggaaa aaactctttt
     5701 cggcccggat cattccctac cgcggctcgt gggttgagtt caacgttgac gtaaacgacc
     5761 tcctctactg ccacatcgac tcacgccgga agctcgccgg cacgacgctc ctgcgggccc
     5821 tgggctacgc caccgacgag gccatcacga gtctcttcta cgaaaccgac gaagaggacc
     5881 tctccggccg caagggctcc aaggccgtcg gcaaaatatc cgccgcgagc gtcgttgacc
     5941 aagctaccgg tgaaatcatc ctccaggcgg gcgacccgat caccgaagag attttagaga
     6001 aaatcaagca ggccaaaatt gagagtctcc gcgtcatcaa aagcgacctc tcccgcgacg
     6061 ctggcgtgat cctcaacacc ttgaagaaag atgctgccca ctcgcaggaa gaagcactct
     6121 taaagattta tgcattgatc cggcccggcg aaccgccgac gccggaggtg gcgttcggcg
     6181 tcttagaacg gctcttcttc aatgccaagc gctacgatct gggcaaggtt gggcgctaca
     6241 tgatcgacga gcgcttaaac ctcgacattc ccctggactg caccgttctg gacaagcgcg
     6301 acttcatcgc catcatcaaa tacctcctgg acctgcgcaa cggcttgggc gacgtggacg
     6361 acatcgacca cctgggcaac cggcgcaccc ggacggtggg ggaactgctc tccaaccagt
     6421 tctcggtggg actttcgcgg atggcccgga ccatgcggga acggatgtcg ctcaaagaca
     6481 ccgattccat caccccccac gacctggt
//
LOCUS       DBNGZA010000175         2146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1622760, whole genome shotgun sequence.
ACCESSION   DBNGZA010000175 DBNGZA010000000
VERSION     DBNGZA010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1622760"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="rfbD"
                     /locus_tag="AC16FU_03645"
     CDS             complement(<1..795)
                     /gene="rfbD"
                     /locus_tag="AC16FU_03645"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR2993500.1"
                     /translation="MKTVLVTGAGGLLGQKLVGVLAPEWHVVAASHRPFEWQEQNVTP
                     EHLDIAIAAESQRIFEQHSPAVVINAAAYTDVDGAEDHREEAHRANVTGPENLARLCR
                     QMNISLVHISTDYLFDGENGPYAEEANPNPLGYYAKTKLWGEEAIIKSGCRYLIVRSN
                     VLYGTAPGVKRNFVLWVIDELQNRRTIRVVSDQYNNPTLADNLAEAIREAVEKGITGL
                     LNIAGSTYLSRHEFALAIAQRFGFDKKLVVPMLTADFKQKAPRPLRG"
     gene            complement(792..1826)
                     /gene="rfbB"
                     /locus_tag="AC16FU_03650"
     CDS             complement(792..1826)
                     /gene="rfbB"
                     /locus_tag="AC16FU_03650"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR2993501.1"
                     /translation="MAGQKQKILVTGGCGFIGSNFVRHLLTTRDDLSLINLDKLTYAG
                     NPENLADLQEHPNYRFIHGDICDGKQVEAVLSEGIDAVVNFAAESHVDRSLYDPAVFI
                     RTNVGGTQVLLEQAVKHKVRRFVQISTDEVYGDLEPQALATESFPLRPSNPYAASKAG
                     ADLLAFSFHRTFGLDVVITRCGNNYGPYQFPEKLIPLFITNALEDKPLPLYGDGLNVR
                     DWIYVTDHCRGIELVMERGRSGKVYNISARTQKNNRDITSAILKALDKPENLIQPVKD
                     RPGHDRRYANDPGKIERELKFKPRFPFEKALAATIQWYRDNRTWWERVKSGEYQKFYE
                     KHYSASGRLK"
     gene            complement(1844..>2146)
                     /locus_tag="AC16FU_03655"
     CDS             complement(1844..>2146)
                     /locus_tag="AC16FU_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR2993502.1"
                     /translation="YHKHQIDTFTVIKGMMKFVLYDSRENSPTKGEVNEFFVGEHNPL
                     LVQIPSGVYHGFKYISDTEAIAINIPTEVYHYKDPDEYRLPAHTDQIPYKWAREDK"
BASE COUNT          416 a          598 c          631 g          501 t
ORIGIN      
        1 gccccgcagt ggccggggtg ctttctgctt gaagtcagct gtcagcatcg gaacaacgag
       61 ttttttatcg aaaccaaaac gttgagcgat tgccagagca aattcgtgtc gggaaaggta
      121 ggtgctacca gcgatgttca gaagaccggt gatccctttc tccacggctt cacggatggc
      181 ctctgccaga ttatccgcca gagtcggatt attatactgg tcgctcacga cgcgaatcgt
      241 cctgcggttc tgtaactcgt cgataaccca gaggacgaaa ttgcgtttga ctcccggcgc
      301 ggtgccgtac agcacgttgc tgcggacgat aaggtacctg cagccgcttt tgatgattgc
      361 ttcctccccc cagagtttcg tcttggcgta atacccaaga ggattgggat tcgcttcctc
      421 cgcgtacggg ccgttttcac cgtcgaaaag gtaatcggtg gaaatgtgca cgagggaaat
      481 gttcatctgg cggcacagcc gggccagatt ctccggcccc gtgacattgg cacggtgggc
      541 ttcttcccgg tgatcttctg caccatcgac gtcggtgtag gcggcggcat tgatgacgac
      601 cgcgggtgaa tgctgctcaa agatgcgctg gctctctgcg gcaatagcaa tatccagatg
      661 ctcaggggtg acattctgtt cttgccactc gaacggtcgg tgtgaagccg ccactacgtg
      721 ccactccgga gccagaacgc ccaccagttt ttgacccagg aggccgcctg cgcccgtgac
      781 tagaacggtt ttcacttcag tcggcctgag gcggaataat gcttttcgta aaacttctgg
      841 tactcgcccg atttcacccg ctcccaccag gtgcggttat cgcggtacca ctggatcgtc
      901 gcggccaggg ccttctcgaa cggaaaccgg ggtttgaact ttaattcgcg ctcgattttt
      961 cccgggtcgt tggcgtagcg ccggtcgtgt cccggccggt ctttcaccgg ctggatcaaa
     1021 ttctccggct tgtccaacgc cttaagaatc gcgctggtga tgtcacggtt atttttttgc
     1081 gtccgggcgc tgatgttgta gactttcccc gaccggcccc gctccatcac taattcgatg
     1141 ccccggcagt ggtcggtaac gtaaatccag tcgcggacgt tcaacccgtc gccgtagagg
     1201 ggcagcggtt tgtcttccag cgcgttggtg atgaagaggg ggatcagttt ttccggaaac
     1261 tggtatggcc cgtagttgtt cccgcaacgg gtgataacga cgtccaatcc gaacgtgcgg
     1321 tgaaaggaga acgccagcag atcggccccg gccttggagg ccgcgtacgg gttcgaggga
     1381 cgcaggggaa aagattcggt ggccagcgcc tgcggctcta aatcgccgta cacctcgtcg
     1441 gtcgaaatct gcacgaagcg cctgacttta tgtttgaccg cctgctctaa gagcacctgg
     1501 gtgccgccga cgttcgtccg gatgaagacc gccggatcgt agagactgcg atcgacgtgc
     1561 gactcggcgg caaaattgac gacggcgtcg atgccctcgg agagaaccgc ctccacctgc
     1621 ttcccgtcgc agatgtcgcc gtggatgaag cggtaattgg ggtgctcttg caggtcggct
     1681 aagttttcgg gattgccggc gtaggtcagt ttgtcgaggt taataagtga gagatcgtcg
     1741 cgggtggtca aaagatgccg gacgaagttc gacccgatga acccgcaacc gccagtgacg
     1801 agaattttct gcttttgacc cgccacacct cacccatcgc ccgtcacttg tcttcccgcg
     1861 cccacttgta gggaatctga tcggtgtgcg cagggaggcg atactcatcc gggtctttgt
     1921 aatggtacac ttccgtgggg atgttgatgg caatcgcctc ggtgtccgag atgtacttaa
     1981 agccgtggta gacgccgctg ggaatctgga ctaatagcgg attgtgctcg cccacgaaaa
     2041 actcgttcac ctcacctttg gtgggggaat tctcccgcga atcgtacagg acgaacttca
     2101 tcatcccctt gatcaccgta aaggtgtcga tctgatgctt gtggta
//
LOCUS       DBNGZA010000176         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2046161, whole genome shotgun sequence.
ACCESSION   DBNGZA010000176 DBNGZA010000000
VERSION     DBNGZA010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2046161"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC16FU_03660"
     CDS             <1..542
                     /locus_tag="AC16FU_03660"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2993503.1"
                     /translation="RGVRILTNTKVVGMEPGKVLIEPGEPIPAETLVWTGGIRASRLL
                     AGSGLPVGVQGRILVDEYLEVKGFPGVFAVGDNALAINPKTGKTVVAAAQFALQQGRL
                     VAWNIFAEMFGKPKKVYVPKVFGEVISLGRHLAVGWIALPGIKRIRFFGFLASLLKRA
                     ITEKHLLLLWRETRNWARA"
     gene            527..1339
                     /locus_tag="AC16FU_03665"
     CDS             527..1339
                     /locus_tag="AC16FU_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR2993504.1"
                     /translation="MGARVISQPSNAAGAPLAASFGWPPWVKFHEQSGAPWLEFEPLK
                     RVPDLLHGFTLRSSNPASSENRREQLKEHLQRIGLPRLEIFLPKQVHGNGVWIIGDAP
                     ERAAPPSVSSDAIVSGRSGLWLGVQVADCLPIFLVDPAKRVVTLIHAGWRSTFLGVIP
                     KTLLTLGDSFASTPDRLVAVLGPTLQSCCAEVGPEVALLFPTKNRVARNSAWVVDFPG
                     MVREQLLAGGLSSGKIFVLPVCTRCGGELFCSYRREGREAGRMFAYAGFVQK"
     gene            1427..2041
                     /locus_tag="AC16FU_03670"
     CDS             1427..2041
                     /locus_tag="AC16FU_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993505.1"
                     /translation="MLKKKMVLWGSLITAGVILSASLQAVAGMGIGVRGGWVNGYKNP
                     AVAGQLAPAQTSLPKQMQMVGLHVKLGTVPVLDLEGSLEYAWKEKKFTYLSNNYNFRL
                     SDISANATAEYVLPFPVLKPHIGAGLGLHRIVYSVKDPQAPYPFPLPGDGTKVGYHAL
                     VGVTFHPPLFPLEFFGDWRYTWVTTTGKQTRYSTLWAGVTLGLP"
BASE COUNT          517 a          699 c          769 g          555 t
ORIGIN      
        1 ccagaggcgt ccgtattctg acgaatacga aagtagtggg tatggagccg ggcaaagtcc
       61 tcattgaacc aggcgagcca atcccagccg agactttggt ctggacgggg ggcatacggg
      121 ccagccgact tctggccggc agcggactgc cggttggtgt tcagggacgc attctggtgg
      181 atgagtacct cgaagttaaa ggatttccgg gcgtcttcgc ggtcggcgat aacgccctgg
      241 cgattaatcc gaagaccgga aaaaccgtag tggctgcggc ccagttcgcc ctccagcaag
      301 gacggctggt ggcctggaac atttttgccg agatgtttgg aaaaccgaag aaagtgtacg
      361 ttcccaaagt cttcggggag gtcatcagtt tggggcgcca cttggcggtc gggtggatag
      421 ccctgccggg tatcaaacgc atccgatttt tcggtttttt ggccagtttg ctcaagcggg
      481 ccatcaccga aaagcatctc ctgttgctct ggagggagac caggaattgg gcgcgcgcgt
      541 aatctcccag ccgtcaaacg cggctggagc accacttgcc gcaagttttg gctggccacc
      601 ctgggtgaaa ttccacgagc aaagcggagc gccttggctt gagtttgagc ccctcaaacg
      661 agttcccgat ttgctgcacg gattcactct ccgctcaagt aatcctgcct cgtcagaaaa
      721 ccgccgggaa caactaaaag agcacctcca acgaatcggc ctgccccgcc tggaaatttt
      781 ccttcccaaa caggtgcatg ggaacggggt ctggatcatt ggtgacgctc cggagcgagc
      841 tgcaccgcct tcggtctcga gcgatgcaat cgtctccggt cgctccgggc tctggctcgg
      901 cgtgcaagtc gccgattgct tgccgatttt tctggtcgat ccggccaaac gagtcgtcac
      961 cctcattcac gccggctggc gcagtacttt tctcggagtg attcccaaaa cgcttttgac
     1021 cctgggcgac tcctttgctt ccacccctga ccgtctagtg gcggtcctgg ggccgacctt
     1081 gcagtcctgc tgcgcggaag tcggcccgga agtagccctc ctctttccga cgaaaaatcg
     1141 ggttgcccgg aacagtgcct gggtggtaga tttccccggg atggtgcggg agcaattgct
     1201 tgcgggaggt ctctcatccg ggaagatttt tgttttaccc gtctgcaccc ggtgcggcgg
     1261 tgaattgttt tgctcctacc ggcgggaggg gagggaggcc gggcggatgt tcgcctatgc
     1321 agggtttgtt caaaaataag gagcggtcaa ggacaccccc gcttattccg atactaactg
     1381 atagccgctc tggcagttag gccaagagca accaagagga gggaagatgc tgaaaaagaa
     1441 aatggttctc tggggaagtc tcatcactgc gggagttatc ttgtccgcct cactgcaagc
     1501 cgtagctggt atgggaatcg gcgtacgcgg cggatgggtc aacggctaca aaaaccctgc
     1561 cgtagcaggt caattagctc cggcccagac cagtctgccc aagcagatgc agatggtcgg
     1621 cctgcacgtc aagctgggca ccgtgcccgt tctggacctg gagggctctc tggagtatgc
     1681 ctggaaggag aaaaagttca cctacctgtc caataactac aacttccggc tctcagatat
     1741 ttccgccaat gccaccgcag agtacgtttt gcccttcccg gtgctcaaac cgcatatcgg
     1801 tgcgggtttg ggactgcacc gcatcgtcta ctcggtcaag gatccgcaag caccgtaccc
     1861 ctttcccctg ccgggtgatg ggaccaaagt cggttaccac gcgctcgtgg gagtgacgtt
     1921 tcacccgccc ctgtttccgc tggaattctt cggcgactgg cggtacacct gggtcaccac
     1981 caccggcaag cagacgagat acagcaccct ttgggccggg gtaactttag ggttaccgta
     2041 agcgccccgc aagctgccac ctcgctgaga gttcttactt accgcctggt catggcgggc
     2101 ggcaccatat tgtcttttcc ggatatctga cgttcccaac ctggtcaccg ccggtggtga
     2161 cgtaattcct gtccgagtca tcctcgggaa aaaaagtaaa ggccgtcacc cccggcgggc
     2221 atttgtattg acaagccagg atcaatcagg tatacttatc cgccagattt gtgtggttgg
     2281 cttttgtgaa gcgtgcggaa cgaggcaaag gagtctctcc ggtcggcagg agccaggtga
     2341 cgtcgttgaa aggagcgaat taatgttgcg tacgatgaga ttcggtgtcg tggcattcgt
     2401 ggcgtgcatt ggaatggccc gggcacagaa agatcaattc ggcgtggtcg ataccgtttc
     2461 ggtggctgtg gagaagggga aaggcgaagg gaactgggtg gcgacggtgg ctttggccaa
     2521 cgacgaaaaa ttgcagggcc
//
LOCUS       DBNGZA010000177         3543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_32412, whole genome shotgun sequence.
ACCESSION   DBNGZA010000177 DBNGZA010000000
VERSION     DBNGZA010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_32412"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16FU_03675"
     CDS             <1..431
                     /locus_tag="AC16FU_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR2993506.1"
                     /translation="VAQKAGEKLKLETLRLLKSDIRYKEIEKRQTLSDDELVAVLSTA
                     AKRRRESIEEFKKGGRADLVAKEEAELGFILGYLPQQLSAEELKELAARAIEEVGAKT
                     QSDVGKVMKVLMPQVRGKADGKTVNACVQALLEATPSPST"
     gene            534..1184
                     /locus_tag="AC16FU_03680"
     CDS             534..1184
                     /locus_tag="AC16FU_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR2993507.1"
                     /translation="MNWVDLVVLLLVIGGAAYGAKRGLIREVMSVLGIVVGIVLAIHH
                     HDAVAMTFLSKMKASPLFVSFLSFLIVLGVSYLGFKILGFALARAASVNQLGNADKVG
                     GAIVGATKAWIIVGFIFLLLFYLPLPDSMLKAVDSSFIAPAMLGTMPVLYEGTKMFHP
                     SGANFLAKLKAPLTRASNKKAEQIQGGMLLPGDWQNDRTEKCLERLDAHFAVKPQQ"
     gene            complement(1284..2036)
                     /locus_tag="AC16FU_03685"
     CDS             complement(1284..2036)
                     /locus_tag="AC16FU_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993508.1"
                     /translation="MTFVQYIPIFTTLFSIFFSWRIFRHYRERKSGPHLLWWGAGIGI
                     YGLGTLAESLVTLFGWHAGLFRLWYITGALLGGAPLAQGTVYLLLKRTTANRLTAVIV
                     PYFLFAATCLLLAPLQNQPADVRILTGKVIGLPDGSWSWVRFLSPFMNTYAVIFLIGG
                     AILSALRYKKDPTMRHRFVGNVFIAVGAILPGVGGAFSRMGHTEVLYVTEFIGLLLIW
                     RGYRLCVKKQEGAEPTLDVRRAVKELLPQEKT"
     gene            complement(2392..>3543)
                     /locus_tag="AC16FU_03690"
     CDS             complement(2392..>3543)
                     /locus_tag="AC16FU_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR2993509.1"
                     /translation="ALYDTSHTYTTVLTVGANLQQIYTLSGKQTFSWGADYYREKIEG
                     FTRDFTHITSPFFSSNTLTQSASVPENHLDALGFYLQDDLQPFERFSGHLGARFDWYR
                     QVTDYTNGYNDISTGRPITPQRKNQTAVSGSAGAQYQVIAGVRLSGNVGTAFRIPNLV
                     EKFFSGTQDQQLVIPNPDLDPEKNISADLGVKFRFDRFSGSVTAFHNQLRDFIELRAT
                     GDSVTKSGRRQAVWHYENISKVRFQGVEAELEAKLPRGFFSYGNASYQRGDNLSTDRA
                     LYVAPAKVVLGLGWKAPQGQFEAEVNDRLILDQKRVDPDPSAVFQLPTPGFDLLSLQT
                     SYTWRWWTFNFNIHNLTNRKYSEPLNAASPFNPITEPGRNFIFGLTTRF"
BASE COUNT          761 a          970 c         1105 g          707 t
ORIGIN      
        1 aagtcgcgca gaaagccgga gaaaaactga aattagagac gctgcggctt ttgaaatcgg
       61 acatccgcta caaagaaatt gagaaaagac agaccctttc ggatgacgaa ctggtcgccg
      121 tgctctccac ggcggccaaa aggcgccggg agtcgatcga agagttcaaa aaaggcggac
      181 gggcagactt agtagccaag gaggaagccg aattgggctt cattcttggc tacttgcctc
      241 agcagctgtc agcggaagaa ttaaaggagt tggccgccag agccatagag gaggtgggag
      301 caaagacaca gtcggacgtc ggtaaggtta tgaaagtgct gatgccgcag gtgcgcggca
      361 aggccgacgg caaaacggtc aacgcctgcg tgcaggcgct tctggaggcc acgccaagtc
      421 ctagcacata gaccgaccat cgtgcgccgg ccagtgaggg tgaaacattt cacgttttga
      481 ccgatacgct aagtagagga acaggacgtg aacttttcat cggaggaggg gaaatgaact
      541 gggtcgatct ggtggtgcta ttgctggtta tcggcggcgc cgcctacgga gccaagcggg
      601 ggctcatccg ggaagtcatg agcgttctcg gtatcgtggt gggaattgtt ctcgccattc
      661 accaccacga cgcagtggcg atgacctttc tctccaaaat gaaggcctcg ccgcttttcg
      721 tttcgttttt gagtttcctc atcgtgctcg gagtttccta cctcggcttt aagattttag
      781 gattcgcttt agcgcgggcc gcctcggtga atcaactggg gaacgccgat aaggtcggcg
      841 gcgccatcgt cggcgcgacc aaggcctgga tcatcgtcgg cttcattttc ctgcttttgt
      901 tctacctgcc cctgccggat agcatgctga aagcggtgga cagctccttc atcgccccgg
      961 caatgctggg cacgatgccg gtgctctacg aaggcacgaa gatgttccac cccagcgggg
     1021 cgaatttcct ggccaagttg aaagctccgc tcacgcgggc gtcgaacaag aaagccgagc
     1081 agattcaggg cgggatgctt ttgccgggag actggcagaa cgaccgcacc gaaaaatgcc
     1141 tggagcgctt ggacgcgcac ttcgccgtca agccgcagca gtagagactc cctggcctta
     1201 acgtattaaa ggaatcgtag ggcggcccgt acgggccgcc tttctttttt tccctcccct
     1261 tgaggggagg attaaaggag gggtcaggtt ttttcttgcg gcaatagttc ttttactgcc
     1321 cggcggacat cgagagtagg ttcggctcct tcctgcttct tcacgcacag ccggtatccc
     1381 cgccagatca aaagcagccc gatgaactcg gtgacgtaga gcacttcggt gtgccccata
     1441 cgggaaaaag cgcccccgac cccgggcagg atggcgccga ccgcaatgaa gacattgccg
     1501 acgaagcggt ggcgcatcgt cgggtctttc ttatagcgca aagccgagag gatggcgccg
     1561 ccgatgagga agatcacggc ataggtattc atgaacgggc ttaagaagcg cacccaggac
     1621 cagctgccgt ccggcagacc gatgaccttg ccggtcagga tgcgcacgtc tgccggttga
     1681 ttctgcagcg gggccaggag caggcaggtg gcggcaaaca agaaataggg aacgatgacc
     1741 gccgtcagcc gattcgcggt cgtccgcttg agcagaaggt agaccgtccc ctgcgccaaa
     1801 ggcgcgccgc ccaaaagcgc tccggtgatg taccagaggc ggaagagtcc cgcatgccac
     1861 ccgaagagag taactaaact ttccgccagc gttcccaacc cgtagatgcc gatgcccgcg
     1921 ccccaccaga ggaggtgcgg cccgctcttt cgctctcgat agtgccggaa aatccgccag
     1981 gagaagaaaa tcgagaaaag cgtcgtgaaa attggaatgt actggacaaa cgtcatagtt
     2041 cgatcacttt ccgcttaatc tcaacttgtc acccgggcga ccgcgggcgt ggattctgta
     2101 ttaggctgta gtcgagactt acatgacctc accccgaccc tctccttcag gagagggggt
     2161 aaatttccct cccctttgag gggagggtga gggaggggtc aagaggtcac caaccaaaga
     2221 tctctcttgg tgaggcggcg agggcgccgc gcccacggca gtgaaatgga acgccgcgtg
     2281 gggcgaccgc tctcggtcgc ccggaacgga gataggtaga ccggtttgtc cctgagccgg
     2341 tcgaaaattg cggcggttca actcgtcgaa aaccacaaca ggaaatgcct ctcaaaaccg
     2401 cgtcgtcaaa ccgaagatga aattccttcc cggctcggtt atcgggttga aggggctggc
     2461 cgcgttcaag ggctcgctgt atttccggtt ggtcaggttg tggatgttga aattgaacgt
     2521 ccaccagcgc caggtgtaac tcgtctggag ggaaaggagg tcaaatcccg gcgtcggcaa
     2581 ctggaagacc gcggaaggat cggggtccac ccgcttctgg tcgagaatca accgatcgtt
     2641 cacttcggcc tcgaactgtc cctggggcgc cttccagccc aggccgagga caaccttggc
     2701 cggcgccaca tagagcgccc ggtcggtgga gaggttgtcg ccccgctggt aggacgcgtt
     2761 tccgtaactg aagaatcccc gtggcagctt ggcctccagt tccgcttcca cgccctgaaa
     2821 gcgcactttg ctgatgttct catagtgcca gaccgcctgc ctgcggccgc tcttggtcac
     2881 cgaatcgccg gtggcgcgca gttcgatgaa atcgcgcagc tggttgtgga aggcggtgac
     2941 cgagccggag aaacggtcga agcggaattt cacccccaga tcggcgctga tattcttctc
     3001 ggggtctaag tccgggttgg ggatgaccaa ctgttgatcc tgggttccgg agaagaactt
     3061 ctccacgagg ttgggaatgc ggaaggcggt gcccacgtta ccgctcaagc gtacgcctgc
     3121 gatcacctgg tactgcgccc cggcgctgcc gcttacggca gtttgatttt tcctttgcgg
     3181 cgttatcggc cggccggtcg agatgtcgtt gtacccattg gtgtagtcgg tgacctgccg
     3241 gtaccagtcg aagcgggcgc ccaggtggcc ggagaagcgt tcgaagggct ggaggtcatc
     3301 ctgcaggtag aaacccaggg catccagatg attctccggc accgatgccg attgcgtcag
     3361 cgtattcgat gagaagaaag gcgaggtgat gtgggtgaag tcgcgggtga acccttcgat
     3421 cttttcacgg tagtaatcgg caccccacga aaaggtctgc ttgccggaga gggtgtaaat
     3481 ttgttgcaaa ttggcgccga cagtcaggac ggtcgtgtag gtgtgactcg tgtcgtacag
     3541 ggc
//
LOCUS       DBNGZA010000178         2258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2152714, whole genome shotgun sequence.
ACCESSION   DBNGZA010000178 DBNGZA010000000
VERSION     DBNGZA010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2152714"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..715
                     /locus_tag="AC16FU_03695"
     CDS             215..715
                     /locus_tag="AC16FU_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993510.1"
                     /translation="MTQRRTGPPALFGKRSYTSLCKKVVWFSLFGAAVLLAFPKNANG
                     WSYPKDGRFKKIKYDPWFYVDERWDIRAPDKFVHAMGSYASCEVLSRVFESRPRWQVA
                     AFVMLSDVYKEYGDAFREGWSPRDLMADALGIGMWIVNRKTAGSRFYVRYDTEKVQFI
                     WALPVR"
     gene            complement(917..1714)
                     /locus_tag="AC16FU_03700"
     CDS             complement(917..1714)
                     /locus_tag="AC16FU_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993511.1"
                     /translation="MTKLFAIFEDTLREAKSRKIFLGFFIFALLVLVVVALGAQFVHR
                     SAGLQVNPTGAKLTGREMSNGVASFLSDILVFLSIFATASVFPHLLEKGTAEIYLSKP
                     VSRGQWLYGKFFSVVGVIAVFVAFVLALVWLVFFLRFGSAWAGYFLFILSTALKFAIL
                     FAFVTCITVLSRSSALALILTFILWVITGFLAVREGLYRVLKTGPVQTVLDWAYYILP
                     KPGEIDKIGEALVRGNPVPSYLPVWSSVLFAVALLLLTHRIFLKKDY"
     gene            complement(1711..>2258)
                     /locus_tag="AC16FU_03705"
     CDS             complement(1711..>2258)
                     /locus_tag="AC16FU_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR2993512.1"
                     /translation="MDRWRKTQLKKYSKGMQQRLGLAVAMVNDPDLLLLDEPTDGVDP
                     VGRKEIRDLLERLKEQGKTIFLNSHLLSEVEMVCDRVAILNQGKLIKVGTVGSLTEQQ
                     CEYMIEVTAWSEAVEGRLREATRAFSRQGNRLSVTLSEPKAINPVIDLLRINKMEILS
                     VIPRRETLEESFLALIARPPQ"
BASE COUNT          527 a          638 c          653 g          440 t
ORIGIN      
        1 accggaggga agtgttaaca atgaggaggg tattcggcta agattagcgc tttcgagcct
       61 cgggagtggt gctcgctggc tttccaacca gccacctgac ccaagtgtcg ggcatccccc
      121 ctccaccact cccctaagcg cggcgggttc agcgacggac ccgccttttt ttatggtcaa
      181 gagcgtgccc tcgaaatgaa agcgaaagga acagatgacc caaagacgaa cgggccctcc
      241 tgccctgttc ggcaaaagaa gttacacctc cctctgtaag aaagtggtct ggttctcgct
      301 tttcggcgca gccgtgctgc tcgcctttcc caaaaacgcc aacggctggt cgtaccccaa
      361 ggacggacgc ttcaaaaaaa taaaatacga tccctggttc tacgtggacg agcgctggga
      421 catccgcgcg ccggacaaat tcgtccacgc catgggctcc tatgcttcgt gcgaggttct
      481 ctcccgcgtt tttgagagcc ggccccgctg gcaggtggcg gccttcgtta tgctgtccga
      541 cgtttacaaa gagtacggtg atgccttccg cgagggctgg tccccgcgcg atttaatggc
      601 ggatgccctg ggcatcggca tgtggattgt caaccgcaaa accgccggtt cgcgcttcta
      661 cgtccggtat gacactgaga aagtgcaatt catctgggcg ctgccggtga gataaagcgg
      721 gacagggaat acggcataga gaacacggga aaaaccattt ccggaaatgt ccgacgggga
      781 ctggcccgat acggctcgta ccgagaagcc ccggctatgg aaaatcccta acatctctgt
      841 acgagtaatc accgaccaat tccataggtc aggcttccag tcccgcccgt agcgggactt
      901 ccagcctgac cggaagctaa taatccttct tgaggaaaat gcggtgcgtg aggagcagaa
      961 gggccaccgc aaaaagcacc gaactccaca ccggcaggta ggacggcacg gggtttcccc
     1021 gcaccaacgc ttcgccgatt ttatcaatct ctcccggttt ggggagaatg tagtaagccc
     1081 agtctagaac ggtttgcacc ggtccggttt taagcactcg atacaaccct tcccggacgg
     1141 ccagaaatcc ggtgatgacc cagaggatga aggtcagaat caacgccagg gccgacgaac
     1201 gcgaaagaac cgtgatgcag gtgacgaagg cgaagagaat ggcgaacttg agcgccgttg
     1261 aaagaatgaa gagaaaatac cccgcccagg cgctgccgaa gcgcaaaaag aagaccagcc
     1321 agaccagagc cagaacgaag gcgacgaaca cggcgataac gccgaccacc gagaagaact
     1381 tgccgtagag ccactgcccg cgcgagaccg gcttggagag gtaaatctcc gccgttccct
     1441 tctccaacag gtggggaaag acgctcgccg tggcgaagat ggaaaggaag actaatatgt
     1501 cggagaggaa actcgccacg ccgttggaca tttcccggcc cgtcagtttt gccccggtag
     1561 gattgacctg caagccggcg cttcggtgga cgaactgcgc gcctaaggcc acgacaacca
     1621 gtaccagaag tgcgaagatg aaaaagccca gaaagatttt gcggcttttc gcctcgcgca
     1681 gggtgtcttc aaaaatggcg aataatttgg tcattgcggc ggtcgggcga ttaaggcgag
     1741 gaaactctcc tccagcgtct cccggcgcgg aatgacggag agaatttcca ttttgttgat
     1801 gcgcagaagg tcaatgaccg gattgatggc tttcggttcg gacagagtga cggagagccg
     1861 attcccctgg cgggagaagg ctctggtcgc ttcgcgcaag cgtccttcca ccgcctcgga
     1921 ccaggcggtg acttcaatca tatactcgca ctgctgttcg gtgagcgagc cgacggtgcc
     1981 gactttgatc agttttccct ggttgagaat cgccacccgg tcgcaaacca tttcgacctc
     2041 ggacagaagg tgcgagttca agaaaatcgt cttcccctgt tctttcagtc gctccaggag
     2101 gtcgcggatt tctttgcgcc ccaccggatc gacgccgtcg gtgggctcgt ccaagagcag
     2161 gaggtcggga tcgttaacca tggcgacggc gagaccgagc cgctgctgca ttcctttgga
     2221 atattttttc aattgcgtct tgcgccagcg gtccattt
//
LOCUS       DBNGZA010000179         9000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_137543, whole genome shotgun sequence.
ACCESSION   DBNGZA010000179 DBNGZA010000000
VERSION     DBNGZA010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_137543"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="AC16FU_03710"
     CDS             <1..2096
                     /locus_tag="AC16FU_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993513.1"
                     /translation="NYMKGLQPDGSPLVNPVTNQVTTFFVSGDPVAGTGWLDNAPADR
                     RMQLTSGPFTFAPGDTQEVVAAIIVGQGGDRLSSITVLKFFDKSAQKAFDLNFVLPSP
                     PDAPAVTVHRLDSKIQLTWTDRSEIQHGDLPFQGYNIYQTRSLIKPDWKLIAVQDVND
                     GLGLVFDDVLDPVTNVVVNKPVAFGSDAGVSHFIDISQDKVLGGPLHNGTPYYFAVTA
                     YSADPNQAPTFRVLETALNPIVAIPEGPVAGSDLTPAKVDTAVIYSRINPALSPSTDV
                     VTAEVFNPEEVTGHTYEVRFHPNQIVSIDSSVVPPETTFQVIPFQNSNGKSVYVTELW
                     DLWDLNTNTAVLTNRWNKAGDNNYDVVGGILVKVQGAYKAELQDVFHVNTGAHRRAWS
                     GVNAGLQFFFNSADYANNFFCSKYDPAVSTNPFTTVQIIFYPNDTARWQYAYNYRRDV
                     VGPNNYRNIGYSRVPFQVWDVVNNLQLNAAFIENARPPVDGNLDSLWDPTDSTTGGRE
                     YVFVFRSTYSPTPDTFYLNHNINSECDIFDMLYAWWPLKRSATDTVDNGDYLEFLFAN
                     PSTANDRYTIRTTAPVLTNAGLAKGQLSRVKAVPNPYFNRSAYELTQFDRQIKFTNLP
                     TTCTIKIYNLAGELVRTLQKTDPSTSQLTWDILSEARLPIASGIYLWRLEAPGIGEAQ
                     GKMVIFTEQEQLNNF"
     gene            2117..3199
                     /locus_tag="AC16FU_03715"
     CDS             2117..3199
                     /locus_tag="AC16FU_03715"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993514.1"
                     /translation="MKKLWMGIAGLTLLLGGVEPVRAGNSNRIGTAGAQELRIPVAAR
                     SVALGGAVVANATGAEALYWNPAGAATLENTEVLFTHLRYIGGMDLNYGAVMHNFGSA
                     GNLGLSAKVLSVGDIEVTNEANPDGTGEIISPTFTTFGAHFARAMTDRVSLGASISVV
                     DERVMREQATGLAFDFGFQYQSGWHGLKLGLVIKNLGSQMRFDGPDFDLNIVPPGADP
                     GSNNRTTRTQSAAFDLPSSMQLGFAYDWLNGGENRVTTMAVFQSNNFSEDEYRIGGEY
                     AYRENYFLRASFVTNAQEVRAWWGRHINKPQSESQQQEGTYLYGAAFGGGVQAKIGDV
                     KAVFDYTFALVQNLEDNHFFTVKFLF"
     gene            3322..3588
                     /gene="rpmE"
                     /locus_tag="AC16FU_03720"
     CDS             3322..3588
                     /gene="rpmE"
                     /locus_tag="AC16FU_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR2993515.1"
                     /translation="MKEGIHPKYYDCVVACACGNRFTTRSTLKEIHVEICSNCNPFFT
                     GKQKLVDTAGRVERYRRKYAPKMETPEPDKPKAARKTQTTTKAH"
     gene            3724..4674
                     /locus_tag="AC16FU_03725"
     CDS             3724..4674
                     /locus_tag="AC16FU_03725"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR2993516.1"
                     /translation="MPDKNIGGQAVIEGVMIRAPRRVATACRKPSGEIVLRSEDYLPL
                     VKRIPFLRVPILRGLVAFLEMLVIGLKSLNFSASVAAEAAQKAGRKNPGKKSAADSFL
                     LFSTTVLAFAIAIAVFFYLPLLAANLFGIRRAQWAFNLAAGGIRVSLFIGYVWFLSLF
                     RDFRRVFEYHGAEHMSIFAYENDEELSTANIRKYPPEHPRCGTSFLMIVALGAIIIYS
                     FSDTIFFWVAGRAPSVPQRFLVHFSLLPFVAGSAFELLKLAGKTRENRLTKILIAPGL
                     WLQKITTRMPHDDQIEVAVAALHSAVTAAPASPLPVSQKA"
     gene            4677..5771
                     /gene="prfA"
                     /locus_tag="AC16FU_03730"
     CDS             4677..5771
                     /gene="prfA"
                     /locus_tag="AC16FU_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR2993517.1"
                     /translation="MLEILEKAEKRYAELSEKLSDPKVLTNQNLYRQVAREHHELEEI
                     VRTGTEFRKVLTAIDDDERIKTESADRELVDLAKGELSELYARREALEANLKRLLIPR
                     DPNDKKSVIVEIRAGTGGEEAALFAAELYRMYTKYAERHAWKIDVMNTNPTGLDGLKE
                     VIFGVTGEGAYGKLKYESGVHRVQRVPVTEASGRIHTSAASVAVLPEAEDVEIDIKPD
                     ELQIDVFRSSGPGGQSVNTTDSAVRITHKPSGLVVSCQDERSQLKNKIKALKILRARL
                     FDKAQREQKEKIDSARRAMVGSGDRSEKIRTYNYPQNRVTDHRIGLTLHKLEDVLSGE
                     LDEFIEGLQTQEQAERLSEAGQIVNEKKAE"
     gene            5866..6513
                     /locus_tag="AC16FU_03735"
     CDS             5866..6513
                     /locus_tag="AC16FU_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HIR2993518.1"
                     /translation="MIRIVIAAIAARLLSKQWNDLQKKLFFQYTRLLNEQEYVPSVLL
                     QRLLISGEDHRFFKHPGFDVIAICRAVWRCVFWGVREGASTIEQQLVRVLTGHYELTV
                     GRKCKEILLATLVTRVVPKSDVPALYLQIAYFGWRMNGFRQACIRLNLRPGVISIQDA
                     ALMIARLKYPEPRIAPPQRLVQIFRRHDHLIRLHQRHLGEGRFVALQPRFCHAPI"
     gene            6461..7180
                     /locus_tag="AC16FU_03740"
     CDS             6461..7180
                     /locus_tag="AC16FU_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993519.1"
                     /translation="MKVVSWLCNHVFVMRPFEHSESAVSLINPRPSADELVKVAKNAW
                     SSELFAIVRQWLSEGIPYAFAHRPFLYETTREFLASRIGIHPKQVTIIGSARLGYSLA
                     PGLNFGKAFNEQSDLDFSAISDDLFERCVNDFRQWRADYKGGTILPAGPRQEGFWKSN
                     FLDVPHNIDRGFIDPWKIPYLPRYHIAQKIGDAMSFVYKKLKVTPNSPSFSKISIRVY
                     KDWYSFVRQVHLNFLSAIRNL"
     gene            complement(7246..8223)
                     /locus_tag="AC16FU_03745"
     CDS             complement(7246..8223)
                     /locus_tag="AC16FU_03745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2993520.1"
                     /translation="MSLPESEAKILDAYSQAVTKVADTVGPAVVNITTSRSPKPYWFE
                     PVPNERAGIGSGVIVRAEGYIVTNAHVVSRSPGLTVTLTDGRRFPARLIGRDRRFDLA
                     VLKIDATRLHAAQLGDSHQLKVGQLVVAIGNPFGLEWTVTAGVVSSLGRTLRLPGGFV
                     AENVIQTDASINPGNSGGALCDSTGKVIGITTAVIYGAQGIGFAIPGETVREVFHEIV
                     EGRTIQKAWLGISGFNQALESEVVAAFNLPSRTGVLVVEVVPNSPADRGGIQQFDIIV
                     AVGGTQTTTVRALQEALRQTVISEPVPVTILRGHQWLQVSVKPEVSPVG"
     gene            complement(8303..8794)
                     /locus_tag="AC16FU_03750"
     CDS             complement(8303..8794)
                     /locus_tag="AC16FU_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993521.1"
                     /translation="MTKKLLSFVVIGMFLLALASFATAGDKPAGKMMGSPNCPACAEG
                     MACEKALKGMGAAMEWVDLSNGLACITTAASPSKATAVQTHMQKHGQKMDELMKTQRD
                     KFCDMCKQMSALIEAGNQQVVNFKGGSMWMVTSNDPKTVESLHQMAAECRSMGTQSAE
                     KKL"
BASE COUNT         1985 a         2532 c         2496 g         1987 t
ORIGIN      
        1 acaactacat gaaggggctg cagccggatg ggagtccact ggtcaatcct gtcaccaatc
       61 aggtgaccac cttcttcgtc tccggcgatc ctgttgctgg caccggctgg ctggataatg
      121 ctcccgctga ccgccgaatg caacttactt ccgggccctt tacctttgcc ccgggcgaca
      181 cgcaggaagt cgtggcagcc atcatcgtcg ggcagggcgg cgaccggctc agttccatta
      241 cagtactgaa attcttcgac aaatccgccc agaaggcttt tgacttgaac tttgtcctgc
      301 catcgccacc ggatgctccg gcggtgacgg tgcaccggtt ggacagcaag attcagttga
      361 cctggaccga ccggtcggag attcagcacg gcgacctgcc gttccagggg tacaacattt
      421 accagacccg ctcgctcatc aaaccagact ggaaactcat tgccgtccag gatgtcaatg
      481 atggactggg gctggttttc gacgatgtcc ttgacccggt caccaacgtg gtggtcaaca
      541 aaccggtcgc tttcggcagt gacgcagggg tgagtcattt cattgatatt tctcaggaca
      601 aggtgttggg tgggccgctc cacaatggga cgccctacta ctttgcggtg accgcttatt
      661 ccgccgatcc gaaccaggcg ccgaccttcc gggttttgga aaccgcactc aatccgattg
      721 tagccattcc ggaagggccg gtggcgggga gcgatctgac gccggcaaaa gtcgatacgg
      781 cagtgatcta cagccgaatc aacccggcat tgtccccttc cactgacgtc gtgacggcgg
      841 aagtgttcaa tcctgaggaa gtgaccggcc acacctacga ggtgcggttc cacccgaatc
      901 agattgtctc catcgactcg tcggtcgttc caccggagac gacattccaa gtgatcccct
      961 tccagaactc caacgggaag tccgtctacg tcactgagct ctgggacctg tgggatttga
     1021 acaccaatac cgccgttttg accaatcggt ggaacaaggc gggggacaac aactatgatg
     1081 tggttggtgg catcctggta aaagtgcagg gtgcgtacaa ggccgaactc caggacgtat
     1141 ttcacgtaaa caccggggct caccggcggg cgtggtctgg cgtgaacgcg ggcctccagt
     1201 tcttcttcaa cagtgccgac tacgcaaaca acttcttctg cagcaaatac gaccccgcgg
     1261 tcagcaccaa cccattcacc acggtacaga taatcttcta ccccaacgac accgcacggt
     1321 ggcagtatgc gtacaactac cggcgggacg ttgtgggacc taataactac cggaacatcg
     1381 ggtactctcg ggtccccttt caggtgtggg acgttgtgaa caatctccag ttgaatgcgg
     1441 cgttcatcga gaacgcaaga cctccggtgg atggcaacct cgattcgttg tgggatccca
     1501 ccgactccac gacgggagga cgtgaatatg tcttcgtctt ccggagcacc tattctccca
     1561 cgcccgatac gttctacctc aaccataaca tcaacagcga gtgcgacatt tttgacatgc
     1621 tctacgcctg gtggccactc aaacgttccg ccactgatac cgttgataac ggggactacc
     1681 tggaattcct cttcgcgaac ccctcaaccg ccaatgaccg ctacaccatc cggacgacgg
     1741 cgccggtact gactaatgcc ggtttggcca aagggcaact gagccgggtg aaggcggtcc
     1801 cgaaccccta cttcaaccgg tcggcctacg aactcacgca gtttgaccgg cagattaaat
     1861 tcaccaacct gccgaccacc tgcaccatca agatttacaa cctggcggga gaactggtgc
     1921 gcacattgca gaaaaccgac cccagtacca gtcaattaac ctgggacatt ttgtcggaag
     1981 cccggctacc catcgcctca gggatttacc tctggcgcct ggaggcgccg gggattggtg
     2041 aggcccaggg caagatggtg atcttcaccg agcaggaaca actcaacaac ttctaaggga
     2101 gaggagagcg agcgcgatga aaaaactatg gatgggaatc gccggactga ccctcctttt
     2161 gggaggggtt gagcccgtac gggccggaaa cagcaaccga attgggactg ccggagcgca
     2221 ggagctgcgc attccggtgg cggcccgctc cgtggccctg ggaggcgcgg tcgtggctaa
     2281 cgctaccggc gccgaagcgc tttactggaa tccggcggga gccgccactt tggaaaacac
     2341 ggaggtgctc ttcacccacc tgcgctacat cggcggcatg gatctcaact acggcgcggt
     2401 gatgcacaac ttcggcagcg cgggcaacct ggggttgtcg gccaaagtgc tctccgttgg
     2461 cgacatcgaa gtgaccaatg aggccaaccc ggacgggacg ggcgaaatta tctcgcccac
     2521 ctttaccacc tttggcgctc acttcgcccg ggcgatgacc gatcgggtct cgctgggagc
     2581 gtccatttcg gtcgtggacg agcgggttat gcgggagcag gccaccggtt tagctttcga
     2641 ttttggcttc cagtaccagt ccggctggca cggtctcaaa ctgggactgg taatcaagaa
     2701 cctgggctca caaatgcgct ttgacggtcc ggattttgat ctcaacatcg ttcctcccgg
     2761 agctgacccc ggttcgaaca accggaccac ccggacgcag tcggcggcct tcgacttgcc
     2821 gtcttcgatg cagttgggct tcgcctacga ctggctaaac gggggtgaga atcgcgtcac
     2881 gacgatggcg gtgttccagt cgaacaactt ctccgaagac gaataccgca tcggcgggga
     2941 gtacgcctac cgcgaaaact acttcctgcg ggccagtttt gtgaccaacg cccaggaggt
     3001 gcgggcctgg tggggccggc acattaacaa gccgcagagc gaatcacagc agcaggaggg
     3061 gacgtatctc tacggggcgg ccttcggcgg cggtgtccag gccaagatag gtgacgttaa
     3121 ggccgtcttc gactatacct tcgccctggt gcagaacttg gaagacaacc acttctttac
     3181 ggtgaaattc ctcttctaag gcatccgcct caggcggatc aattgagaaa aatccccccg
     3241 ccactggcgg ggggattttt ctttccggct caattgcttt ttggcgtttt gcgtatagat
     3301 taggttgtaa ggaggacagc tttgaaagaa ggcatacacc ccaagtatta cgactgcgtg
     3361 gtagcctgcg cctgcgggaa ccggtttacc acccgctcga cgttgaagga aattcacgtc
     3421 gaaatctgct ccaattgcaa cccgttcttc acgggcaagc agaaattagt ggacaccgcc
     3481 ggccgggtgg agcgctaccg ccggaagtac gcgcctaaaa tggaaacgcc ggagccggac
     3541 aaacccaaag cggcgcggaa aactcagacc acaaccaaag cgcattagcg taacgaagcg
     3601 ggcggggttc ggtgcgttac cgttcgtccc cgcccttttt ctttccgctg ttgtcgggaa
     3661 gaaggccact gcccacacgc agtccgggtg gggtgagcgc tacgaaagat cgggaatcag
     3721 gcaatgccag ataaaaacat cggcggtcag gccgtcatcg aaggggtaat gattcgggcg
     3781 cctcgacggg tggccactgc ctgccggaag ccctccggcg aaatcgtcct gcgttccgaa
     3841 gactaccttc cgctggtgaa gcgcatcccc ttcttgcggg tgccgattct ccgcggttta
     3901 gttgcctttc tggagatgct ggtgattggc ctcaagagtc tgaacttttc ggcctccgtc
     3961 gccgccgaag cggcccagaa ggcgggaagg aaaaatcccg ggaagaagtc ggcggcggac
     4021 tcctttctgc tcttcagcac taccgttctc gcgtttgcca tcgccattgc ggtgttcttc
     4081 tacctgccgc ttctggcggc caatttattc ggcatccggc gcgcgcagtg ggccttcaac
     4141 ttagcggcgg gcggcatccg ggtatccctc ttcatcggct acgtctggtt cctctcgctt
     4201 ttccgcgact tccggcgggt ctttgaatac cacggcgccg agcacatgtc gattttcgcg
     4261 tatgagaacg acgaggagct ctccaccgcc aacattcgca agtatccgcc ggaacacccg
     4321 cgctgcggca ccagttttct gatgatcgtt gctctgggag cgatcatcat ctactcgttc
     4381 tcggatacca tcttcttttg ggtagccgga cgggcgccct cggtaccgca acggtttttg
     4441 gtccacttct ctctgttgcc cttcgtggcg gggagtgcct ttgagttact gaagctggcc
     4501 gggaaaacgc gggagaatcg cctgaccaag attttaattg cgcccggtct gtggctgcag
     4561 aaaatcacca cccggatgcc ccacgatgac cagattgagg tagcggtggc ggcgttacac
     4621 agcgcggtga cggccgctcc cgcttcccca cttccggtga gtcaaaaggc ataaccatgc
     4681 tggagattct cgaaaaagcc gaaaagcgct atgccgaact ttccgaaaag ctttccgacc
     4741 cgaaagttct gaccaaccag aacctctacc ggcaggtggc ccgggagcac cacgaattgg
     4801 aggaaatcgt ccgtacaggt accgaattcc gcaaggtgtt gaccgccatt gacgatgatg
     4861 agcggatcaa aaccgagtcg gcggatcggg agttggtcga tctggcgaag ggagaattga
     4921 gcgaactcta cgcccggcgc gaggctctgg aagcgaatct caaacggctc ttgatccccc
     4981 gcgatcccaa tgacaaaaaa agcgtcatcg tcgaaatccg ggccggcacc ggcggcgaag
     5041 aagcggcgct ctttgccgcc gaactgtacc gcatgtacac caagtatgcc gaaagacacg
     5101 cctggaagat cgacgtgatg aacactaacc caaccggtct ggatggtttg aaagaagtca
     5161 tcttcggggt caccggtgag ggggcctacg gaaaattgaa gtacgaatcg ggcgtccacc
     5221 gcgtgcagcg tgtaccggtg accgaggcct ccggccgtat tcacacgtcg gctgcgtcgg
     5281 tggccgttct gcccgaggcc gaagacgtag agatcgatat taaaccggat gaactccaga
     5341 tcgacgtctt ccgctcctcg gggccgggag ggcaatcggt gaataccacc gattcggcgg
     5401 tacggatcac acacaaacca tccggcctcg tcgtttcgtg tcaggacgag cggtcgcagt
     5461 tgaagaacaa aatcaaggcg ctcaaaatcc tccgggcgcg gctcttcgac aaggcgcagc
     5521 gcgagcagaa ggaaaaaatc gactcggccc ggcgggcgat ggtcggctcc ggcgatcgct
     5581 cggagaaaat ccgcacctat aactatccgc agaaccgggt gaccgaccac cgcatcggcc
     5641 tcaccttgca caaattggaa gacgtgctct ccggcgaact ggatgagttc atcgaaggcc
     5701 tccagacgca ggagcaggcg gagcggcttt cagaagctgg gcagattgtc aatgaaaaaa
     5761 aagctgaatg atgttgttct tgacagttgc gtagggtact catttaatcc ctactgacag
     5821 atctttaacg gtaaagaccg cagttttgga attaaaggga gaagattgat aaggattgta
     5881 atagcagcca tcgcggcaag acttctcagt aaacaatgga atgatttgca gaagaaacta
     5941 tttttccaat acacacgatt gttaaatgag caagagtatg ttccctcagt attgctgcag
     6001 cgtcttctta tttcaggaga ggatcaccgc tttttcaaac acccagggtt tgatgtaatt
     6061 gccatttgcc gcgctgtttg gaggtgtgtt ttttggggtg tccgagaagg ggcaagtacg
     6121 attgaacagc aactggtacg cgtccttaca ggacactacg agttaaccgt cgggcgaaag
     6181 tgtaaagaaa ttcttttggc aactttggtc acgcgtgttg ttccgaaatc cgatgttccc
     6241 gcactttatt tacagattgc ctattttggt tggcgaatga acgggtttcg tcaagcttgc
     6301 ataaggttaa atcttcgacc aggagtaata tcaattcagg atgcggcttt gatgatagcg
     6361 agattgaaat atccggagcc acgcattgca ccacctcagc gccttgttca gattttcaga
     6421 cgccacgacc atttgattag gctccatcaa aggcatctcg gtgaaggtcg tttcgtggct
     6481 ctgcaaccac gtttttgtca tgcgcccatt tgaacattca gaatcagcgg tctctttaat
     6541 taatccccgt ccttcagccg atgagttagt caaggttgca aaaaacgcgt ggtcttcgga
     6601 gttatttgct atcgttaggc aatggctgtc ggagggaatt ccatatgctt tcgcacaccg
     6661 gccttttctt tatgaaacaa caagagaatt tctagcttcg agaataggaa tacatcctaa
     6721 acaagttacc atcattggaa gtgcgcgtct tgggtattca cttgctccag gattaaattt
     6781 tgggaaagcg tttaatgaac agtcagactt ggattttagc gcgatctctg atgatttgtt
     6841 tgaacggtgt gtaaacgact ttagacaatg gagagcggat tacaagggtg gtacgatttt
     6901 gcccgctgga ccacgacagg aaggcttttg gaaaagcaat tttttggatg tgccacataa
     6961 tattgatcga ggttttattg acccgtggaa aattccttat ttgccccgtt atcatatcgc
     7021 gcagaagatc ggcgatgcaa tgtcctttgt atacaagaag ctaaaggtca cacccaatag
     7081 cccatctttt tcaaaaatct ccatccgtgt ttataaagac tggtattctt tcgttcgaca
     7141 agttcatcta aattttttgt ccgctatccg aaatctttga tctctcccac tgccttcggc
     7201 gagagtctat cgaccctcaa ggggaggtaa accaggtaca gaatctcatc ccaccggaga
     7261 gacttccggt ttgacgctca cctgcagcca ctggtgcccg cggagaattg ttaccggtac
     7321 tggttcgctg attacggtct ggcgcagggc ttcctgtaaa gctcgcacgg tagttgtctg
     7381 cgtgccgccg acggcgacga tgatgtcgaa ttgctggatg ccgccccggt cagccgggct
     7441 gttcggtacc acttccacca ccagaacgcc ggtgcgggaa ggcaggttga acgccgccac
     7501 cacctccgat tccagcgcct ggttaaatcc gctgatgccc agccaggcct tctgaatcgt
     7561 cctgccttcc acgatttcgt ggaacacttc gcgcaccgtt tctccgggga tggcaaaacc
     7621 gattccctgc gcgccgtaaa tcaccgccgt cgtaatcccg atgactttac cggtagagtc
     7681 gcacaaggcc ccgccggagt tgccgggatt gatggaggca tcggtctgga tcacattttc
     7741 ggccacaaag ccgccgggca aacgcagtgt ccgtcccaga gaactgacga cgccggcggt
     7801 gaccgtccac tccaatccaa acggattacc gatggccaca actaactggc cgacttttaa
     7861 ctgatgcgaa tcgcccaact gcgcggcgtg gaggcgggtc gcatctattt taaggactgc
     7921 cagatcgaac cgccggtcgc ggccgataag ccgggccgga aaccggcggc cgtcggtcag
     7981 ggtaacagtt aagccgggac tgcggctgac tacgtgggca ttggtaacta tgtacccctc
     8041 ggcgcggact atgacgccgg agccgatacc ggcgcgctcg ttcggcacgg gctcaaacca
     8101 gtaaggtttt ggggagcgtg aggtggtgat gttgaccacc gcggggccga ccgtatcggc
     8161 cactttcgtc acggcttggg agtaggcgtc taagattttt gcttccgatt cggggagcga
     8221 catagaaccc tttcttaagc aagaaaaacc ggggcagaga gtgtaccgcc ccggcccaca
     8281 cactgtcccg ccattggcgg gattacagct tcttctctgc ggactgcgtt cccatagagc
     8341 gacactcggc cgccatctgg tgcagcgatt ccacggtctt cgggtcgttc gaggtgacca
     8401 tccacatcga gccacccttg aagttgacca cttgctggtt gcctgcctcg atcaaggcag
     8461 acatctgctt gcacatatcg cagaacttgt ccctctgggt cttcatcagt tcatccatct
     8521 tctgcccatg tttctgcata tgtgtctgga ccgccgtcgc tttggagggc gaggccgcgg
     8581 tcgtgatgca ggccaagccg ttggagaggt ccacccattc catggcggcg cccatgccct
     8641 tcagggcctt ctcgcaggcc atcccttccg cacaggctgg acagttcggt gagcccatca
     8701 tcttcccggc cggcttatcc ccggcggtgg caaaggacgc cagagccagc aagaacatcc
     8761 cgatgacgac gaatgacaat agcttcttgg tcatttcctg ttttcacctc cttccgtaga
     8821 gttaaggttt tggagtttat ttcccggttt catttcagac caaatttctc ttgatagacg
     8881 gagtaagcag agtatttgtt agacttttct tttcagatgt ctccaaccgc ccggatgcta
     8941 atactttttc tctgaaaaag caagagcaat tggcggcggg cgggtgcatt tgacattcct
//
LOCUS       DBNGZA010000180         5206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1727733, whole genome shotgun sequence.
ACCESSION   DBNGZA010000180 DBNGZA010000000
VERSION     DBNGZA010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1727733"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16FU_03755"
     CDS             <1..188
                     /locus_tag="AC16FU_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993522.1"
                     /translation="DWGFGAAAPAKKTWRLRRHRNFYQIRTAEGDEYEMYLDRGAKGE
                     KESWVLLRQVSSGSPAT"
     gene            196..837
                     /gene="plsY"
                     /locus_tag="AC16FU_03760"
     CDS             196..837
                     /gene="plsY"
                     /locus_tag="AC16FU_03760"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR2993523.1"
                     /translation="MAKLAGIILAAYVLGALPFSLWLARLWKGIDIRRHGSGNPGATN
                     VFRVLGPVPGIGALLLDAGKGFLAVAVVSHFWSDALPTSQMVTSLLTAVAVVLGHIYS
                     FFLGFKGGKGVAPATGAMLALLPMEAGAGIMIFLFVVAISRYVSLGSLVSSACVALLV
                     GGEKFLLKKSLDPLLVGTCLFLTVVIFYTHRANIRRILAGTEHKVGQKDKQVS"
     gene            853..1860
                     /locus_tag="AC16FU_03765"
     CDS             853..1860
                     /locus_tag="AC16FU_03765"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010631652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR2993524.1"
                     /translation="MATCAILGAGSWAIAVAGLLDGNGHTVRLWEFDPAEAERLRTTR
                     QQPQKLPGIRLPESVLVSNVPAEVLREAHVVLLALPSHIIREVARRLRDLIPRDALVV
                     NLAKGLEIEPLFRMSEVLRSELPAALQKKIVTLSGPSHAEEVSRQMPTSVVVAGTSPE
                     AVQKTQELFANNFFRVYTNADLIGVELAGSLKNIIAIAAGMVDGLGFGDNTKGALLTR
                     GLAEITRLGEKMGAKPATFAGLAGLGDLVTTCLSRHSRNRYVGEQIGKGKTLQQVLAG
                     MVMVAEGVKTTEAAYRLARRHGVEMPITEQVHCVLFENKSPRAAVQDLMTRTLKAEVW
                     T"
     gene            1999..2340
                     /locus_tag="AC16FU_03770"
     CDS             1999..2340
                     /locus_tag="AC16FU_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR2993525.1"
                     /translation="MAEAPEKLYDSISEVAEMVGVKPYVLRFWEKEFPTLKPKKNNAG
                     NRMYQKKEIEFARQIKELLYERGFTIAGARTNLKTERKEARTNPHHPEATVRLVKEIR
                     KDLSDLLKLLT"
     gene            2380..2453
                     /locus_tag="AC16FU_03775"
     tRNA            2380..2453
                     /locus_tag="AC16FU_03775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2414..2416,aa:Pro,seq:ggg)
     gene            2521..3204
                     /locus_tag="AC16FU_03780"
     CDS             2521..3204
                     /locus_tag="AC16FU_03780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993526.1"
                     /translation="MPPKSGKVAIIIPAYNAEKTIGEVVKRCAEYVPVTDVVVVNDGS
                     ADGTGAKARASGAVVLMHPANRGKGEALKTAFAYAIEKKYTGVVTLDADLQHYPQYVP
                     NFVQTAQRARADVLVGSRPRTFRNMPFSRVVTNTLTSILISITSGQVVRDSQSGYRYF
                     TTEALKKLRPQSSRYDLESEMLIQAGRCGLRIAAVPISTVYEGSRSFINPFKDTGRFI
                     RILWRSLWW"
     gene            3320..4087
                     /gene="surE"
                     /locus_tag="AC16FU_03785"
     CDS             3320..4087
                     /gene="surE"
                     /locus_tag="AC16FU_03785"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR2993527.1"
                     /translation="MLVLITNDDGIQAFGLRARAKEMSKLGRVLIVAPDREQSATSHA
                     LTLHRPLRLNKLAGNVYSVDGTPTDAVMIAVHGILKHRRPDILLSGINHGPNMGDDVT
                     YSGTVAAAIEGTLLGIPSLAISYALGAVRRNSLALRDFALPARIARTVALRMLKHLLP
                     SGTFLNVNIPWVKNGKLRGCEITRLGSRVFNDIIIAKTDPRGKRYYWIGGEPTWKAKT
                     RSDFSALQKKKVSITPLKTDLTNYEALPGLASWRLPL"
     gene            4205..4432
                     /locus_tag="AC16FU_03790"
     CDS             4205..4432
                     /locus_tag="AC16FU_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HIR2993528.1"
                     /translation="MALKACIFVQTTGDPVTVARKIRNVTGIAEATPLFGSVPIIAFA
                     KATNLATLHRLVMKIKKLKGVTDTDTRLAVQ"
     gene            4513..>5206
                     /locus_tag="AC16FU_03795"
     CDS             4513..>5206
                     /locus_tag="AC16FU_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR2993529.1"
                     /translation="MLTLLVVLPIQKSLAYWQQEVHYQIEATLVDEKARKFTGHETLR
                     YVNHSPDTLRVVYFHLYLNAARRGSRMDQRRQEFFDYGLADSKPEQWGEERIDWLEDG
                     IDRPLKVTVDETIARVELAGPLPPGAECAFEMQFTSIFPSVSIGRTGFVDGQFKGGYW
                     YPTVAVYDHKMGWVNNQFLGAGEAYGEFGTFDVSLTFPAHFIVAATGLLQNRNEVLPE
                     SLCQKLRIENFKD"
BASE COUNT         1124 a         1451 c         1520 g         1111 t
ORIGIN      
        1 aggactgggg ttttggcgca gcagctccgg caaagaagac ttggcgtttg cgccgacacc
       61 ggaactttta tcagatacgc acggcggaag gggacgagta tgagatgtac ctggatcgcg
      121 gcgcgaaagg tgaaaaagag tcgtgggtgc tcctgcgcca ggtgagttca gggtctccgg
      181 ctacttgagc agtcaatggc taaacttgcg ggaataattc tcgctgccta cgttctcggt
      241 gctttacctt tcagtctctg gctggcccgc ctctggaaag ggatcgacat ccgccggcac
      301 ggttctggca atccgggagc aacgaacgtc tttcgggttc tggggccggt cccgggcatc
      361 ggcgcacttc ttttggatgc gggcaaagga tttctggcgg tagcggtggt aagtcatttc
      421 tggtcggacg cgcttcccac cagccaaatg gtaaccagtt tgctcaccgc cgtcgcggtc
      481 gtcctgggtc atatctattc gttctttctc gggttcaaag gcggcaaggg ggtagctccg
      541 gccaccgggg caatgctggc gctcctgccg atggaagccg gtgctgggat tatgattttt
      601 cttttcgtcg tcgccatcag ccgctacgtt tccttgggtt ctctggtttc gtcggcctgc
      661 gttgccctgc tcgtcggagg ggaaaagttc cttctcaaaa aatcacttga tccgcttctg
      721 gtcggtactt gccttttcct gaccgtggtg atattctaca ctcaccgggc caatatccgg
      781 cggattctgg cgggaacgga acataaagtt gggcaaaaag ataaacaggt ttcttaaagc
      841 ggaactgtaa gcgtggccac ctgtgcaatt ctgggagccg gctcgtgggc aattgccgtc
      901 gccggtttat tggatgggaa tggccacacg gtgcggctgt gggaatttga tcctgccgaa
      961 gccgaacggc tccgcactac tcgccagcag ccgcaaaaac ttcccgggat ccgcctgcct
     1021 gagagtgttt tggtcagcaa tgttccggct gaggtgttgc gggaggcgca cgttgtcctc
     1081 ttggctttgc catctcacat catccgggag gtggcccgcc gcctgcgcga tttaatccct
     1141 agagacgccc tggtggtaaa tttggctaag ggtctggaaa ttgaaccgct attccggatg
     1201 tccgaggtac tgcgttcaga attgcccgct gcattgcaga aaaagatcgt gacgctttcc
     1261 ggaccaagcc atgccgaaga agtcagccga cagatgccca cctcagtcgt ggtggcgggc
     1321 acgtcacccg aagcggtgca gaaaacgcag gaactgttcg cgaacaattt ctttcgcgtc
     1381 tacaccaatg ctgatctgat tggcgtggag ttagccggat cgctgaaaaa tatcatcgcc
     1441 atcgcggcgg ggatggtgga tggcctgggt ttcggcgaca atacgaaagg tgccctactc
     1501 acccgcggtc tggcggagat aacccggctg ggtgagaaaa tgggtgcgaa gcccgcaacg
     1561 tttgccggtc tcgccggtct gggtgacctg gttaccacct gcctctcgcg tcacagccgg
     1621 aaccggtatg ttggtgaaca aatcgggaaa gggaagaccc tccagcaggt tctcgccggc
     1681 atggtgatgg tagccgaggg ggtcaaaacc accgaggcgg cctaccggct ggcgcgccgg
     1741 cacggagtcg aaatgcccat caccgagcag gtacattgcg tgctgttcga gaacaagagt
     1801 ccccgcgctg ccgtgcagga tttgatgaca cgcacgctta aggcggaagt gtggacgtag
     1861 aagcaggtgg tgggtcggag actcgtccac ccccctgtag cgcaggttgg tttctgaaac
     1921 ctgctcccga gaagagcgga atacgtgcct cgacttcgct cggcataaga aaaccattcc
     1981 gcgccaccaa gtgacactat ggctgaagcg ccggaaaaac tctacgactc gatcagcgaa
     2041 gtcgccgaaa tggtgggcgt caagccctac gtactgcgtt tctgggaaaa ggaattcccc
     2101 actctcaagc ccaagaagaa taacgccggc aaccggatgt accagaaaaa agaaattgag
     2161 tttgcccgtc agatcaaaga actgctgtac gaacgcggtt tcaccatcgc cggcgcccgc
     2221 accaatctca aaaccgaacg caaggaagcc cgcaccaatc cccaccaccc ggaagccacc
     2281 gtacggctgg tgaaggagat ccgcaaagat ctttcggatt tactcaaact tctgacttga
     2341 gtttgtctct ataaaaccta tcttacccaa agcgaatgtc ggggcgtagc gcagcctggt
     2401 agcgcactcg cttggggtgc gagtggtcgg ccgttcaaat cggctcgccc cgataaagac
     2461 agtgattagt gatgagtgat tagggattgg ccaaagaaaa acctgccgag tgttgccatt
     2521 ttgccgccaa agtcgggaaa agtcgccatc attattccgg cgtacaatgc ggagaaaacc
     2581 ataggggaag tagttaaacg ctgtgccgaa tatgttccgg tgacagacgt ggttgttgtc
     2641 aacgatggct cagccgacgg gaccggagcc aaagcgagag cgtccggggc tgtggtgctg
     2701 atgcacccag ccaatcgcgg caagggggag gcgctgaaaa ccgccttcgc ctatgcaatt
     2761 gaaaagaagt ataccggggt cgtcacgctt gatgccgacc tacagcacta tccgcaatac
     2821 gttcccaact tcgtgcagac agcccaacgt gcccgggccg atgttctcgt gggcagccgg
     2881 ccacgcactt tccggaatat gcccttctcg cgggtggtga cgaatacgct gacttcaatt
     2941 ctgatctcga ttaccagtgg ccaggtggtg cgggatagcc agtcgggcta ccgctacttc
     3001 acgacggaag cgttgaaaaa gttacgaccg caaagcagcc ggtatgacct cgaatcagaa
     3061 atgctcattc aagcgggacg gtgcggtctg cggattgcgg cggtgccaat cagcacggtc
     3121 tacgagggct cccggagttt cattaatccg ttcaaggaca ccgggcggtt catccgaatt
     3181 ctgtggcggt cgttgtggtg gtgaagattt ttaggcgagc gaggcgctcg ccctacgcga
     3241 ggagaaaagc attatccgta ggcgcggcgc ctcgccgcgt tcaaattagc atgcattgcc
     3301 gcggtaacgg agttttccca tgcttgtctt aatcaccaac gacgacggca tccaggcctt
     3361 cggcctgcgg gcacgggcca aagagatgtc gaaattaggg cgcgtcttaa tcgtcgcgcc
     3421 ggatcgggag cagagcgcca ccagccatgc cttgacgctt caccggccgc tgcggctgaa
     3481 taaactggcc ggaaatgtct actccgtgga tggtacgccc accgatgcgg tgatgatcgc
     3541 cgtccacggc atcttaaaac accggcggcc cgacattttg ctttccggca tcaaccacgg
     3601 acccaatatg ggcgatgacg tgacctactc cgggacggtg gcggccgcca tcgaaggcac
     3661 cctcctggga attccctcac tggccatttc gtatgcgttg ggcgcggtgc ggcgcaactc
     3721 gcttgcgctc cgcgattttg cactgccggc ccggattgcg cgtactgtgg ccctgcggat
     3781 gctcaagcat ctgttgcctt cgggaacgtt tttgaacgtc aacatcccct gggtaaagaa
     3841 cggcaagctc aggggctgcg agattacccg cctgggctcc cgggtgttca acgacattat
     3901 catcgccaag accgacccgc gcggcaagcg ctactactgg atcggcgggg agcctacctg
     3961 gaaggcgaaa acccgaagcg acttctccgc tctgcagaag aagaaagtct ccatcactcc
     4021 cctcaaaacc gacctcacca actacgaggc gttgccgggt ctggccagct ggcgtcttcc
     4081 gctttaagct gtcggggctg tcaccagcat cgggcttcag gttgtgcttt tgtccggcag
     4141 ggctatttta acctgttacc ggtaaggcgt ttttcttatg tagtcagctg atagaggagg
     4201 tccaatggcg ctcaaagcct gcatttttgt tcaaaccacc ggtgatccgg tgaccgtggc
     4261 ccgaaaaatc agaaacgtga ccggaatcgc cgaagcgact ccgcttttcg gctccgtccc
     4321 catcatcgct ttcgccaagg cgacaaacct ggcgaccctg catcgtctgg tgatgaaaat
     4381 caaaaaactc aaaggtgtga ccgacaccga cacccgcctc gccgtccagt agtaaccccc
     4441 gtccccgttt cctgcggctt cgtggagcat gaagggaaaa aagtgacact gtccccaagc
     4501 aggattgttt ggttgctgac gttacttgtt gtccttccca ttcaaaagtc attggcctac
     4561 tggcagcagg aagttcatta ccaaatcgaa gcgacgctgg tcgatgagaa agcccgcaaa
     4621 ttcaccggtc acgagacact gcggtacgta aatcactcac cggatacgct ccgcgtcgtt
     4681 tactttcacc tctatctgaa tgcggcccgc cggggcagcc ggatggacca gcgccgccag
     4741 gagtttttcg attacggcct ggccgattcc aaaccggagc agtggggcga agaacgaatc
     4801 gactggctgg aggacggcat tgaccgtccg cttaaagtga cggtggatga aactatagca
     4861 cgtgtcgaat tggctgggcc gctgccgccc ggcgctgagt gtgcgtttga aatgcagttc
     4921 accagtattt tcccctcagt ttcaattggt cgcaccggat ttgtggatgg tcagttcaag
     4981 ggcggctact ggtatcccac cgttgcggtg tacgaccaca aaatgggctg ggttaacaat
     5041 cagtttttgg gtgccggcga agcctacggc gagttcggga cgttcgacgt gtcgctgacc
     5101 tttcccgccc acttcatcgt cgcggccacc ggcctcttgc aaaatcgaaa cgaagtgctg
     5161 ccggaatcgc tctgccaaaa actcagaata gagaatttca aagaca
//
LOCUS       DBNGZA010000181         6748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_986503, whole genome shotgun sequence.
ACCESSION   DBNGZA010000181 DBNGZA010000000
VERSION     DBNGZA010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_986503"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /gene="rpoB"
                     /locus_tag="AC16FU_03800"
     CDS             <1..1973
                     /gene="rpoB"
                     /locus_tag="AC16FU_03800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR2993530.1"
                     /translation="VSPKQLVSVAAALIPVLAHDDANRALMGANLPRPAVPLLQTDAP
                     VVGTGVERKAALDSGAVVLARRAGVVESVTAERIVIIPSGRGREGELDFSEADEYELD
                     KFRRSNQDTCINQRPLVQVGDRVEKGQIIADGPATDRGELALGANVLVAFLPWRGYNF
                     EDAIVISERLVVDDIFTSIHIEEFELQVRDTKRGAEELTREIPNVSEEAVMNLDERGI
                     VRTGAEVEAGDILVGKVTPKGETELSPEERLLRAIFGEKAGDVRDASLKAPPGMKGVV
                     IDTRVISRKERTDAVKKQEKTEIARIRKQFHQQMEQIRVIRDRRVLEILEGEVSRTLR
                     NKRGDVVLRAGTKFKEDTFTDIDVADLVGEEGFTDSDAKNRRVAQIVLHCEKLLEKKK
                     GQLEVETEKVIRGAELPPGVVQLVKVRVATKRKVSVGDKMAGRHGNKGVVARTVPVED
                     MPYLPDGTPVDIVLNPLGVPSRMNVGQILETHLGWAAQKLGLYVASPVFDGARSEDIA
                     EALKKAGLPPNGKSVLYDGRTSEKFEAPVTVGIIYMMKLSHLVDDKIHARSIGPYSLV
                     TQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQEMLTVKSDDVAGRSKIYEAIVKGE
                     NPPEPGVPESFNVLVKELQSLGMDVQLMEVNK"
     gene            2022..2435
                     /locus_tag="AC16FU_03805"
     CDS             2022..2435
                     /locus_tag="AC16FU_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993531.1"
                     /translation="MGQVIRPDLLRRVRDYWYQEQLMWQFSNCGRTHRVLPVVMAIFL
                     SGCCQLGLTCYELYIIGEPKLNDADIYVDNKLVKSSVNGSATLHLSWETHEVKVVQKG
                     FKPYEIVIEGKGGRSERGLKVKFEPLNAVDTVHTP"
     gene            2505..6566
                     /gene="rpoC"
                     /locus_tag="AC16FU_03810"
                     /pseudo
     CDS             2505..6566
                     /gene="rpoC"
                     /locus_tag="AC16FU_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706650.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            6639..>6748
                     /locus_tag="AC16FU_03815"
     CDS             6639..>6748
                     /locus_tag="AC16FU_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR2993532.1"
                     /translation="MPTIQQLIRFGRRSVSVSSKTPALGGSPQKRGVCTRV"
BASE COUNT         1475 a         1944 c         2141 g         1188 t
ORIGIN      
        1 acgtctcgcc caagcagctc gtgtcggtgg ccgccgcctt gattcccgtt ctggcgcacg
       61 acgacgccaa ccgggcgctc atgggcgcca acctgccgcg cccggcggtg ccccttttgc
      121 aaaccgacgc gccggtggtg ggcaccgggg tggagcgcaa agcggctctg gattccggcg
      181 cagtggtttt ggcccggcgc gccggggtgg ttgaatcggt gaccgccgaa cgcattgtca
      241 tcattccctc cggccgcggc cgggaggggg aactcgattt ctccgaagcc gacgaatacg
      301 aattagataa attccgccgc tccaaccagg acacctgcat caaccagcgt cctttagtgc
      361 aggtggggga ccgggtggag aaggggcaga tcatcgccga cggcccggcc accgaccgcg
      421 gtgaactggc cttgggcgcc aacgtgctcg tggctttcct gccctggcgg ggctacaact
      481 tcgaagacgc tatcgtcatt tcggaacgcc tggtggtgga cgacatcttc acctcgattc
      541 acattgaaga attcgaactg caggtccgcg ataccaaacg cggcgccgag gaactgaccc
      601 gcgagattcc caacgtctcc gaagaagcgg tcatgaatct ggacgaacgc ggcatcgtgc
      661 ggaccggcgc cgaggtggaa gccggcgaca tcctggtggg caaggtcacc cccaaagggg
      721 aaaccgagct ctcgccggag gagcggctcc tgcgggccat cttcggcgaa aaagcgggcg
      781 acgtgcggga cgcatccctc aaagcgccgc ccgggatgaa aggggtggtc atcgacaccc
      841 gcgtgatctc ccgcaaggag cggaccgacg cggtcaaaaa acaggagaag accgaaatcg
      901 cgcgcatccg caagcagttc caccagcaga tggagcagat tcgcgtcatc cgcgaccggc
      961 gcgttctgga aatcttggaa ggcgaagttt cccgcaccct gcgcaacaag cgcggcgacg
     1021 tggtgctccg ggccggcacc aagttcaaag aagacacctt tactgatatc gacgtagccg
     1081 acctggtggg cgaggaaggg ttcaccgaca gcgacgccaa gaaccgccgg gtggcgcaga
     1141 tcgtcctgca ctgcgaaaaa ttgctggaaa agaaaaaggg acaactcgaa gtcgaaaccg
     1201 aaaaagtgat ccggggcgcc gaactgccgc cgggcgtggt ccagttagtc aaagttcgcg
     1261 tggcgacaaa acgcaaggtt tcggtcggcg acaagatggc cggccgccac gggaataagg
     1321 gggtagtggc cagaaccgtc ccggtggaag atatgcccta cctgcccgac ggcacgccgg
     1381 tggacatcgt cctcaatccc ttaggagtac cttcccgcat gaacgtgggc cagattctgg
     1441 agacccacct gggctgggcc gcgcagaaat tgggtctcta cgtggccagt cccgtcttcg
     1501 atggcgcccg gtcggaagac attgccgagg cgctcaaaaa agccggcctg ccgcccaacg
     1561 gcaagtcggt cctctacgac ggccgcacga gcgaaaaatt cgaagcgccg gtgaccgtgg
     1621 ggatcatcta catgatgaag ctctcgcact tagtggatga caaaatccac gcccgctcca
     1681 tcggccccta ctcgcttgtc acccagcagc ccctgggcgg caaggcgcag ttcggcggcc
     1741 agcgcttcgg cgaaatggag gtctgggccc tggaggccta cggcgcggcc tacgcgctcc
     1801 aggagatgct gacggtcaaa tccgacgacg tggccggccg ctcgaaaatt tacgaggcca
     1861 tcgtcaaggg cgaaaacccg cccgagccgg gcgtgccgga gtcgttcaac gtcttggtca
     1921 aggaactcca gagtctgggg atggacgtgc agctgatgga agtgaataaa taagtgtcga
     1981 ggtttcgaag tatcggagtg tcgaggttga gcccgaaaga ggtgggtcag gtcatcaggc
     2041 ctgacctgct tcgtcgggtt agagattact ggtaccaaga gcaactaatg tggcaattct
     2101 ctaattgcgg gagaactcat cgtgttctgc ccgtggtgat ggcaatcttt ctatcaggtt
     2161 gttgtcaact cgggctgacc tgttacgaac tttatataat cggagagcct aagctgaatg
     2221 acgctgatat ttatgttgac aacaagctgg ttaaatcctc tgtcaatggt agtgctactt
     2281 tgcatctttc gtgggagacg catgaagtta aggtggtgca aaaagggttt aagccgtatg
     2341 aaatcgttat tgagggaaaa gggggacgaa gtgagcgagg gcttaaagtt aagttcgaac
     2401 cgctcaatgc ggtggataca gtgcatacac cctgatggtg aaaccataga actttgaagg
     2461 tatttgatgc ttattgagtt caaagaaatt aaggagggag acgcgtggct gacgtaactg
     2521 tccgttcttc ccgacggccg tcggatttct cggccatcaa gattcaattg gcctcgccgg
     2581 agatgatccg gcagtggtcc tacggcgagg tgaccaagcc ggagaacatc aactaccgct
     2641 cgttcaagcc ggagcgggac gggctcttct gcgagcggat cttcggcccg gtgaaagatt
     2701 gggagtgcaa ctgcggcaag tacaaacgga tccgctttcg cggcatcgtc tgcgaccgct
     2761 gcggcgtgga ggtcacccag tccaaagtcc ggcgcgagag gatggggcac atcgaactgg
     2821 cggtgccggt ctcccacatc tggtacttca agtcgctccc ctcgcgcatg gcctacctgc
     2881 tcgatgtatc cattcgcgat ttggaacgca ttctctacta cgaggcctac gtcgtcatcg
     2941 accccggcaa cacttccctc aagcagagcg acatcattac cgaggagcag tacacccagt
     3001 tggaggaagc caaaaaggag tttacggcca aaatgggggc ggatgcgatc tacgagatcc
     3061 tgaagcaaat cgacatcgaa gagctttcga tttccctgcg cgcccaggtg aaagtggaaa
     3121 cctccgtcgc gcgcaagaag gacactttga agcgcctgcg ggtgatcgag gcctttcgcc
     3181 agtcccggaa ccggccggag tggatgatcc tccaggtttt gccggtgatc ccgcccgatc
     3241 tcaggccctt ggtgcctctg gaaggcggac gctttgccac ctccgacttg aacgacctct
     3301 accgccgggt gatcaatcgg aacaaccggc tcaaaaaact gatcgacatc aaggcgccgg
     3361 acgtcatcct gcgcaatgag aagcggatgt tgcaggaagc ggtcgacgca ctcctggaca
     3421 atggccggcg cacccagtcg gtccgcggcg actccaaacg gccgctcaaa tcgctctccg
     3481 accttttgaa agggaagcag gggcgcttcc gccagaacct tttgggcaag cgggtcgatt
     3541 actccggccg ctcggtcatc gtcgtgggcc cggaactgaa actccaccag tgcgggctgc
     3601 cgaagaacat ggctttggag ctcttcaaac cgttcctcat catgaaatta gagtagaagg
     3661 ggtacgtcca aaccgtcaaa tcggccaaaa aattggtcga gcgggagcgg cccgaggtct
     3721 gggacatttt agaggaaatc atctccgacc acccggtgat gcttaaccgg gcgcccaccc
     3781 tccaccggct gggcgttcag gccttctacc ccgtgttagt cgaaggcaaa gcgattcgtt
     3841 tgcacccgtt ggtttgcacc gcgttcaacg ccgacttcga cggcgaccag atggcggtgc
     3901 acgtgccgct ctcgtttgaa gcgcagttgg aagcgcgcgt gcttatgctc tcgacccaca
     3961 atctcctctc cccttccagc ggcaagccga tcgtggcgcc gaaccaggac atcgttttgg
     4021 gctgctacta cctgaccaaa gaccgccccg gggacaaagg tgaggggatg cgcttctggg
     4081 gtccggaaga agtccaggcc gcctgcaatg caggcgtcat cggtctgcac gcgcggatca
     4141 agatgaagct tgacggtgac ctcgtggaga cgaccgccgg ccgggtgctc tttagttcca
     4201 ttctcccgcc ggaattgggt ttcttcaacg aagtggcgag caagggtaag ttggaggatt
     4261 taatcggcca ggcctacaag acgctggggg cctaccgcac cgcggccttc ttggatgaac
     4321 tgaaaaaact gggttttgag tacgccaccg tggccggcgt gacggtgggc ctcgatgact
     4381 tagtcattcc ggaggagaag gagggcttga tcagccgggc cgaaaaggaa gtcgagcgga
     4441 tccggaagca gtacgagcgc gggctcatca ccaacggcga acggtacaac aaggtcatcg
     4501 acacctggac ccacacctcg accgaagtgg ccgagcggat gttcgcccgg ctggaggcgg
     4561 accggcacgg cttcaacccg gtctacatga tggccgattc cggggcccgc ggctccaagg
     4621 agcaaatccg gcagctggcc ggcatgcggg ggctcatggc caagccgcag aagaaaatta
     4681 ccggccagat cggcgaaatc atcgagtcgc caattatttc caatttccgc gaagggctga
     4741 ctgttctgga gtacttcatc tccacccacg gagcgcgcaa agggctggcc gacacagcgc
     4801 ttaaaaccgc cgatgccggc tacctcaccc ggcggctcgt ggatgtggcc caggacgtga
     4861 tcatcaacga agaggattgc ggcacgatcc tggggctttc catcggcgcc ctgaaagagg
     4921 gcgaagaagt gattgagtcg ctcaaagacc gggtcctggg ccgggtagcg ttagaagatg
     4981 tggtggacct gatcactgaa gaagaactgg cccgcgccgg tgaagagatc accgaaaccg
     5041 cggccgagca gatcgaggag agcggcttgg aggcgatcaa gattcggtcg gtcttgacct
     5101 gcgagtcgcg ccggggcgtg tgcgcgaagt gctacggccg gaacctggcc accggccgga
     5161 tggtggactt aggcgaggcg gtgggtgtca tcgccgccca gtcgatcggc gaaccgggca
     5221 cgcaattgac tttgcggacc ttccacatcg gcggcaccgc ggcccgcatc gccgaacagt
     5281 cgaaagcggc ggcgaaaact gacgggcggg tggtcttccg caatctccgt tcggtggccc
     5341 gcgccgccgg agaaacggtg gccatttccc gggatgggga aattaactta gtcgatggcg
     5401 agggccgcct gcgcgcgcgc tacaacgtcc cctacgccgc ggtgctccac gtggagccgg
     5461 aaagccaggt gaagaaagag caggtgctct ttgagtggga cccctatacc tccaccatcg
     5521 tggccgagca gtccggcacg gtgggattcc gcgatttagt cgaaggtgag tcgtaccgcg
     5581 aagagttaga tgagaccacg ggtttgaggc aaagggtggt gatcgagcag cgcgagaagg
     5641 ccctcaaccc ccagattctt ttgaccggcg agcgggagcg gaaactgggc acctacaaca
     5701 ttccgaccgg tgcccacctg caggtgcacg acgggcagaa aattcactca ggcgacctcc
     5761 tggtcaaaat cccgcgggag atcgcgaaga cgcgcgacat caccggcggt ctgccccggg
     5821 tggcggagct cttcgaagcc cgcaagccgc gcgacccggc catcgtttcg gaaatcgacg
     5881 gctacgtgga gttcggcaag cttatccgcg gggccacgca gctcttcgcg cgcggggaag
     5941 acgaggtcaa ggagtaccag atcccgcacg gcaagcacct ccgggtgcac aacggcgacc
     6001 gagtcaaggc cggtgaccgt ctctgtgaag gcccggtgga tccccacgac atcctgcgga
     6061 tcaaaggggt aaatgcggta caggagtatc tcgtcaacga aatccaggaa gtctaccggc
     6121 tgcagggcgt gaaaatcaac gacaagcaca tcgaagtgat cgtgcgccag atgctccaga
     6181 aagtgcggat cgaggagatc ggcgacacca actttctgga aggggagcag gtggataagt
     6241 tccgcttccg ggaggaaaac gagcgggcga ttgccgaggg cggcgaaccg gccacctttc
     6301 agccccagct cttgggcatc accaaggcgg cgctctccac cgacagcttt atctcggccg
     6361 cctcgttcca ggaaacgacg aaagttctga ccgaagccgc aaccaacggg aggatcgaca
     6421 cgttggaagg actgaaggag aacgtcatca tcggccgcct gattcccgcc ggtaccggcc
     6481 tggagcgcta ccgcagcgtc cagctcttag aggaagcaga ggcgcatccc gcgccgttgg
     6541 cggagatggt tgaggagcgt acttgacact ggcgccggag acgttaccat aggtaggttt
     6601 gcgccgatcg taggcgtttg ctactgagga ggaattgagt gccgacgata cagcagctga
     6661 tacgcttcgg acgccggagc gtgagcgtca gttcaaaaac accggccctg ggcggatcgc
     6721 cccagaagcg cggggtctgc acgcgggt
//
LOCUS       DBNGZA010000182         2893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2047334, whole genome shotgun sequence.
ACCESSION   DBNGZA010000182 DBNGZA010000000
VERSION     DBNGZA010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2047334"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1034
                     /locus_tag="AC16FU_03820"
     CDS             414..1034
                     /locus_tag="AC16FU_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993533.1"
                     /translation="MLRTMRFGVVAFLACMGMARAQKDQFGVVDTVSVAVEKGKGEGN
                     WVATVALANDEKLQGLTVPLKYPEGCKVDSVSFADTRLVSFWMKVGNTNTSVQNAVLL
                     FGIATADQNKQYLEPGRGPIARIYFSAPGELAVTAASLDTTRLPLHNVLQLVTPDSKS
                     IYPAFVNGAAVSAPAAAPAASTDTVAAKKAPMPAKTATPTKSKKKK"
     gene            1148..1224
                     /locus_tag="AC16FU_03825"
     tRNA            1148..1224
                     /locus_tag="AC16FU_03825"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1182..1184,aa:Val,seq:gac)
     gene            1416..>2893
                     /gene="thrS"
                     /locus_tag="AC16FU_03830"
     CDS             1416..>2893
                     /gene="thrS"
                     /locus_tag="AC16FU_03830"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR2993534.1"
                     /translation="MTVGRNSPQQYPRGITPAEILQRIRHPKPEAILAAKVNGRVVDL
                     NRPLAADSTVEFLTFDEAEGREVFWHSSAHVMAQAVTELFPGTKLAIGPPIEEGFYYD
                     FDRGEPFSPEDLEQIEKRMRKIVAGAYPFQRTECSREEALERFGPLGEKYKLEILEAI
                     PPDEQVSLYTHATFTDLCRGPHLPDTGGIKAVKLTASSGAYWRGSEKNPMLQRIYGVS
                     YPDPALLDDFLRRMEEAKRRDHRRLGKELKLFTFAEEVGRGLPLWLPKGATVRRILER
                     YIVDLEQKKGYQHVYTPPLANVELYKISGHWDHFRENMYPPLVIEEEELVLRPMNCPH
                     HIMIYKSEPRSYRDLPVRLAELGEMFRYERSGVLTGLHRVRGMVLNDAHIFCAPEQIK
                     KEFQNVVRLILEVYQDLQITDFWHRLSLHDPQDRVKYAQNEEMWRVAEGMLREALDEL
                     GLPYVVAPGEANFYGPKLDVQIKTATGQEETLSTVQLDFHLP"
BASE COUNT          619 a          857 c          856 g          561 t
ORIGIN      
        1 tccgctggaa ttcttcggcg actggcggta cacctgggtc accaccaccg gcaagcagac
       61 gagatacagc acccttttgg ccggggtaac tttagggtta ccgtaagtca gcgcgcacac
      121 ctcgctcgga attctaccgc ctgttcatac cggcgatgcg aagtcgtctt ttccggatac
      181 cagtcgttcc caccctggtg cccccggtgc tgacgtagtt cctgtccgcg tcattcttgg
      241 aaaaaggtaa agaccatcac ccttggcggg catttgtatt gacaagccag gatcaatcag
      301 gtatacttat ccgccagatt tgtgcggttg gcttttgtga agcgtgcgga acgaggcaaa
      361 ggtgtctctc cggtcggcag gagccaggtg acgtcgttga aaggagcgaa ttaatgttgc
      421 gtacgatgag gttcggtgtc gtggcattcc tggcgtgcat gggaatggcc cgggcacaga
      481 aagatcaatt cggtgtggtc gataccgttt cggtggctgt ggagaagggg aaaggcgaag
      541 ggaactgggt ggcgacggtg gctttggcca acgacgaaaa attgcagggc ctcaccgtgc
      601 cgttgaaata cccggagggg tgcaaggtcg actcggtcag ttttgccgac acgcgactcg
      661 tttccttctg gatgaaagta ggcaatacca acaccagtgt ccagaacgcg gtgctccttt
      721 tcggcatcgc caccgccgac cagaacaagc agtacctgga gccgggccgc ggccccattg
      781 cccgcattta tttttcggcc cccggggaac tggcggtgac cgccgccagc ctggatacga
      841 cgcggctccc cttgcacaat gtgctgcagc tggtcactcc ggacagcaag agcatctacc
      901 ccgcttttgt caacggtgcc gctgtttcgg cacctgccgc cgcaccggcg gccagtaccg
      961 ataccgtcgc cgccaagaag gcgccgatgc cggccaagac tgcgacgccg accaagtcga
     1021 aaaagaagaa gtaagttggc ggccctttcc tgcgaaccat tttttccggc accccgttct
     1081 gccgcgtcat ctcgtttcca ggggagtgtg cctgttgcgg ttgtgggaat gtcgccgcag
     1141 cggtcggggg cgattagctc agttggttag agtgccgtct tgacatggcg gaggtcactg
     1201 gttcgatccc agtatcgccc accattgctg cacgaacgga atggcggtgg ttaaggcaat
     1261 aattcccgcg acgataggaa tgagtgaaga gcccggggtc gaccgggatt atggctacgg
     1321 gagaaatccg cgttagtaca caaagatccc ggcacatgtt tttatctatg gtgaaggagt
     1381 tgcagacaat cccagccggt agtaacaaga taatcgtgac tgtcggcaga aactcaccgc
     1441 agcagtaccc gcggggtatc acgcccgcgg agattttaca gcgcattcgc catcccaaac
     1501 cagaggcaat cttggcggcc aaggtcaacg gtcgggtggt ggacctgaac cggccgctgg
     1561 ctgccgacag cacggttgaa tttctcacct tcgacgaagc ggaaggccgg gaagtcttct
     1621 ggcactcctc cgcccacgtc atggcccagg cggtcaccga actcttcccc ggaacgaaac
     1681 tggccatcgg cccgccgatc gaggaaggct tctactacga tttcgaccgc ggcgaaccgt
     1741 tttcccccga ggacttggaa cagatcgaaa agcggatgcg taaaatcgta gccggcgcct
     1801 acccgttcca gcgcaccgag tgctcacggg aagaggcgct tgagcgcttc ggtcctctgg
     1861 gggagaagta caaactggaa attttagagg cgataccgcc cgatgagcag gtcagtctct
     1921 acacgcacgc cacctttacc gacctctgcc ggggaccgca tctgcccgac accgggggga
     1981 taaaagcggt gaagttaacc gcatcctccg gcgcctactg gcgcggctcc gagaagaacc
     2041 cgatgctcca gcggatttac ggcgtttcgt atcccgatcc ggcgctttta gacgatttcc
     2101 tgcggcggat ggaagaagcc aaaaggcgcg accaccgccg gctgggcaaa gaattaaagc
     2161 ttttcacttt tgccgaagaa gtgggtcgcg gcctgccgct ctggctcccc aagggggcga
     2221 cggttcggcg catcctggaa cggtacatcg tcgacctgga acagaaaaaa ggctatcagc
     2281 acgtctacac gccgcctttg gccaatgtcg aactgtacaa gatctccggc cactgggacc
     2341 acttccgcga gaacatgtac ccgccgctcg tcattgaaga ggaggagtta gtcctccggc
     2401 cgatgaactg cccgcaccac atcatgattt acaaaagcga gccgcgctcc taccgcgacc
     2461 tgccggtacg cctggccgag ctgggcgaaa tgttccgcta cgaacgctcg ggcgtcctca
     2521 ccggcttgca ccgggtgcgc gggatggtgc tcaacgacgc ccacattttc tgcgcgcccg
     2581 aacagatcaa aaaggaattt cagaacgtag tgcgcctcat cctggaagtc taccaggatc
     2641 tccagatcac cgatttctgg caccggctgt cgctccacga tccccaggac agggtgaagt
     2701 atgcccagaa cgaagagatg tggcgggtgg ccgaagggat gctgcgcgag gccttggacg
     2761 aactgggact cccctacgtc gttgcccccg gggaggccaa cttctacgga ccgaaattag
     2821 acgtccagat caaaaccgcc accggccagg aggaaaccct ctccaccgtc cagctcgact
     2881 tccacctgcc gga
//
LOCUS       DBNGZA010000183         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1516655, whole genome shotgun sequence.
ACCESSION   DBNGZA010000183 DBNGZA010000000
VERSION     DBNGZA010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1516655"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16FU_03835"
     CDS             complement(<1..371)
                     /locus_tag="AC16FU_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2993535.1"
                     /translation="MRLRGKVAIVTGASRGIGKAIALRLAAEGADVVVAATNVQLIEE
                     TAHEIEAKGVRAKAVRVDVTRAEETKRLVEETSEAFGRVDILVNNAGITKDGLLVRLA
                     EADWDQVLAVNLKGAFLVSKA"
     gene            complement(385..1335)
                     /gene="fabD"
                     /locus_tag="AC16FU_03840"
     CDS             complement(385..1335)
                     /gene="fabD"
                     /locus_tag="AC16FU_03840"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR2993536.1"
                     /translation="MKRALLFPGQGSQYVGMARDWYEAYPEVQNLFAQAEEILGYDLA
                     GICFAGPEERLTQTQHTQPAIFAHSTAAFTVLQSAGLTFDYVAGHSLGEYSALVANGC
                     LSFHDGLSAVKFRSRFMQEACDEKPGTMAAVIGLPADVVVGICREVSNFGRIQPANFN
                     SPEQIAISGERPGVERAVGLAKEKGALRAMLLPVGGAFHSHLMAPAAERLGEVLTTLS
                     FHPAKVPVVANVTAQPTEDPVLIRKLLVDQITCPVRWVETLQFLHQAGVTEFVEIGPG
                     RVLTGLVRRTLKGVATHSIDTTGDWAGLGAKLSQAALTEK"
     gene            complement(1439..>1936)
                     /locus_tag="AC16FU_03845"
     CDS             complement(1439..>1936)
                     /locus_tag="AC16FU_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR2993537.1"
                     /translation="TEPRVGLLSIGEEKSKGNELVLASHRLLAETPVNFIGNVEGRDI
                     LRGQCEVVVTDGFTGNVMLKFAESVYGFLASKFRDQIRSNVFSKVGVFLLGPFLRRMK
                     RTLDYAEYGGAPLLGIDGVCIICHGKSSPLAIKNAIKVAEQMVTEKVNEQIKEQLAAH
                     ALVAT"
BASE COUNT          369 a          621 c          546 g          400 t
ORIGIN      
        1 caggctttgc tcaccaagaa ggcccctttg aggttgactg ccagcacctg atcccagtcg
       61 gcttcggcga gccgtactaa aaggccgtct ttcgtaatac cggcattgtt gaccaaaata
      121 tccacccgcc caaaggcctc gctggtttct tctaccagcc gtttggtctc ctcggcccgg
      181 gtaacgtcaa ctcggactgc cttagcccgg actcctttcg cctctatttc gtgcgcggtc
      241 tcttcaatga gttgaacgtt ggtggccgcc accaccacgt cggcaccttc ggccgccagc
      301 cgcagggcaa tcgccttgcc gatgccgcgc gaggcaccgg ttacgatggc caccttcccg
      361 cgcaggcgca aacttacgct cccctcattt ttctgtcaac gccgcttgcg agagtttggc
      421 gccgaggccg gcccagtcac cggtcgtatc gatgctgtgg gtggcaaccc ctttgagggt
      481 gcggcgcacc aggccggtga gcaccctccc cggtccgatt tcgacgaact ccgttacacc
      541 cgcctggtgc aggaattgca aggtttccac ccagcgtacc gggcaggtga tttgatcgac
      601 taaaagtttc cggatgagca cggggtcttc ggtcggttgc gcggtgacgt tcgccaccac
      661 cggtaccttc gccgggtgaa acgaaagtgt cgtcaggact tctcccaggc gctcggccgc
      721 cggcgccatc aagtgggaat gaaacgcccc ccccaccggc aaaagcattg cccgcagcgc
      781 ccctttttct ttggccagac ccaccgcccg ttcgacgccg ggccgttcac cggaaatcgc
      841 tatctgctcc ggagaattga aattggccgg ctgaatcctg ccgaaatttg agacctcgcg
      901 gcaaatcccg accaccacat ctgccggcag gccgatgacc gccgccatcg ttcccggttt
      961 ttcatcgcag gcttcctgca tgaagcgtga gcgaaacttg accgcggaga ggccgtcgtg
     1021 aaaagataaa cagccgttgg cgaccagggc cgaatactcg cccagactgt ggcccgccac
     1081 gtaatcgaat gtgagacccg cagattgcag gacggtgaag gcagcggtgc tgtgggcgaa
     1141 aatcgccggc tgggtgtgct gcgtctgggt gagccgctct tccggaccgg caaaacaaat
     1201 ccctgccagg tcgtaaccca atatctcttc tgcctgcgcg aaaaggtttt ggacctctgg
     1261 atacgcctcg taccagtcgc gggccatccc cacgtactgg gagccctggc cggggaacag
     1321 gagtgcgcgt ttcatgggat ttgtaaatta ctgaatgctg ggagagactt cacgtttaac
     1381 tttgccaagc gacctggcct tcggcggcgg agaacggtcg gcgaatggcc ggaggacatc
     1441 aggtcgccac cagggcgtgc gcggccagct gttctttgat ctgttcgttc accttttctg
     1501 tcaccatctg ctcggcaact ttgatggcgt ttttaatggc cagaggcgac gatttgccat
     1561 ggcagatgat gcagacgccg tcgattccca gcagcggcgc gccgccgtac tcggcgtagt
     1621 ccagagtccg cttcatccgc cgcagaaaag gccccaagag gaagacgccc accttcgaaa
     1681 agacgttgct cctgatctgg tcccggaatt tcgacgccaa aaacccgtag accgattcgg
     1741 cgaatttcag catcacgttg ccggtgaagc cgtcggtcac caccacctcg cactggcccc
     1801 gcaggatgtc gcgcccctcg acgttgccga tgaagttgac cggtgtttcg gctaagagtc
     1861 gatgcgaggc caggaccaat tcatttccct tgcttttctc ctcaccaatg gacaaaagtc
     1921 ccacccgcgg ctccgt
//
LOCUS       DBNGZA010000184         3316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2576746, whole genome shotgun sequence.
ACCESSION   DBNGZA010000184 DBNGZA010000000
VERSION     DBNGZA010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2576746"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16FU_03850"
     CDS             complement(<1..313)
                     /locus_tag="AC16FU_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993538.1"
                     /translation="MRTADETAHRPGWQTEFFAQFFDWDEPDPDLLWRFKARLDNPLI
                     KTNSDHLIGDEIPREKDPRTYRLLLLGDSSPVGLGLQSHSLAFGELLESLLEREYADR
                     KR"
     gene            664..2016
                     /locus_tag="AC16FU_03855"
     CDS             664..2016
                     /locus_tag="AC16FU_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR2993539.1"
                     /translation="MSKYPSIKTELPGPKSRALIERDERYVSHSYTRAYPTVIDRAEG
                     ALIWDVDGNCFLDFHSGIGVCSTGSCHPKVVEAIRKQLEKAVHIASADFYHEPVGQLA
                     EKMSEIAPGDSDKRTFFTNSGTEAVETAFKLARYNKKKPRMIAFFGAFHGRTMGALSL
                     TNSKATQRRYFAPLVPEVTHVPFGYCYRCPLNLKYPSCNVACVDYIEGEVFTHVAPPE
                     EVAAIFVEPIQGEGGYIVPPPLWLPKLKALAEKYNILLVADEVQSGMGKTGKMFAVEH
                     WGVEPDIIAAAKALASGMPLGACIARSDLMTWERGAHSTTFGGSPVACAAALVTIDLL
                     QTGLMENAQRLGALILERLYEMQKEHWSMGDVRGKGLMIGTEFVYDRETKKPAGELAE
                     AIQMECFRNGLMLLTAGPSSIRFVPPLVIDQPMVEKALEIFEAAVTKVESRKQSAVLV
                     "
     gene            2018..3064
                     /locus_tag="AC16FU_03860"
     CDS             2018..3064
                     /locus_tag="AC16FU_03860"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR2993540.1"
                     /translation="MGQLVPLAAVNRGDVQESVHYGSVVVVDDTGRVIYSAGDPDFIT
                     FFRSASKPFQAIPLVAEGGIERFGLTSQELAIICGSHSGQPEHIQVVEQLLMKVGFIE
                     GDLQCGIHPPSFFTANELTPEPGARFSQLHHNCSGKHTSMLALSKIYDEPKENYLDPN
                     SRAQQAILQAVAEACRYPAVEIGVAIDGCSAPNFALPLRHMARGYANFVTAHGSTPAS
                     SRVYQTLRQAMLEHPLMVSGVGRFDYALMQAGRGQVLSKAGAEALECAALVDRGWGLA
                     VKIADGSQRAVAPVVIEVLSQLGVPLDMSVPKLAEFARPVIKNYRGRVVGQIAPIFKL
                     QAVKPARVREAVAV"
     gene            3083..>3316
                     /locus_tag="AC16FU_03865"
     CDS             3083..>3316
                     /locus_tag="AC16FU_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR2993541.1"
                     /translation="MLIEPYKIKVVEPIRLISRETSEARLKEAYFNLFNLKAEDVYID
                     LLTDSGTAAMSDIQWAGLMQGDESYAGARSFYRF"
BASE COUNT          730 a          914 c          981 g          691 t
ORIGIN      
        1 aacgcttcct atcagcatat tcacgttcga ggagcgactc caacagttct ccgaaagcga
       61 gcgagtggga ttgcagtccc agtcccaccg gggatgagtc ccccaagagc agcaggcgat
      121 aggtccgtgg gtctttttct ctgggaattt cgtcgccgat gaggtggtcg gagttggttt
      181 ttatcaacgg gttatcgaga cgggccttga atctccagag cagatccggg tcgggctcat
      241 cccaatcgaa gaattgcgcg aaaaactccg tttgccaccc cggacggtgc gcggtctcgt
      301 ctgcggtacg cacaacccgc gcttccagta acagggcggt tatctccaga agtacgagaa
      361 atagaaaagt gataaatacg ggaaaaagta ggtctcgtct cacggtattc aatttacttg
      421 gcgcgccgaa tcagtgtcaa tgaaggcgac cggtcacgcg aaacgtacag cgcaagactt
      481 caccgacctg taaccgcggt tgactttttt ccgggggaag agtagtttct tagcaaggta
      541 tttctatgtt ctacgtacta cgccgcatcc tgttccgtat ctggctcaaa gggcagacct
      601 aagcctcggt tggctttttt ccgattgatt cgaagacaga ggagaccaaa ccgaggagat
      661 acaatgtcga aatatccgag cataaaaact gaactacccg gaccgaaaag tcgggcactg
      721 atcgaacggg acgagcggta tgtctctcac tcttacaccc gcgcctaccc gacggtgatt
      781 gaccgggccg agggcgcttt gatctgggac gtggacggca attgcttcct cgacttccac
      841 tcgggcattg gagtctgctc gaccggttcc tgtcacccga aggtcgtcga agcaatccgc
      901 aagcaactcg agaaggcagt gcatatcgcc tcagccgact tctaccacga gccggtaggc
      961 caattagcgg agaaaatgtc cgagattgcg ccgggtgaca gcgacaaacg gacttttttc
     1021 actaactccg ggaccgaagc ggtcgaaacc gccttcaaat tggcacggta caacaaaaaa
     1081 aagccgcgga tgatagcttt tttcggcgcc ttccacggcc ggaccatggg cgcgctttcg
     1141 ctcaccaaca gcaaagcgac gcaaagacgg tactttgccc cgctcgtgcc ggaggtgacc
     1201 cacgtcccct tcggttactg ttaccgctgc cccttgaact tgaaatatcc ctcctgcaac
     1261 gtggcctgcg ttgattacat cgaaggggaa gttttcaccc acgtggcgcc gccggaagaa
     1321 gtggcggcta ttttcgtgga accgattcag ggcgaaggcg gctacatcgt cccgccgccg
     1381 ctctggctgc cgaaattgaa ggccctggcc gaaaagtaca atattcttct ggtggcggat
     1441 gaagttcagt ccggcatggg caagaccggg aagatgttcg ccgtggagca ctggggcgtg
     1501 gagccggata tcatcgccgc agccaaggcg ttggcctccg gaatgccttt gggcgcttgc
     1561 atcgccagaa gcgatttgat gacctgggag cgcggggcac attccaccac ctttggtggg
     1621 agtcccgtgg cctgcgcggc ggccctggtc accattgacc tgctccaaac cgggttgatg
     1681 gaaaatgccc agcgcttggg cgccttgatt ctcgaacgcc tgtacgagat gcaaaaagag
     1741 cactggtcaa tgggagatgt gcgcggaaaa gggctgatga tcggcaccga gttcgtttac
     1801 gaccgggaga ccaaaaagcc ggccggcgaa ctggccgagg cgattcagat ggagtgcttc
     1861 cgcaacggct taatgctttt gaccgccggg ccttcctcca tccgcttcgt gccgcctttg
     1921 gtcatcgacc agccgatggt ggagaaggcg ctggagattt ttgaagcggc agtgacgaaa
     1981 gttgaaagcc gaaaacaatc ggcggtgctg gtttagaatg ggtcagcttg taccgctggc
     2041 cgcggtcaat cgcggagatg ttcaggaaag tgtccactat ggctcggttg tagtggtgga
     2101 cgacaccggc agagtaattt actccgccgg cgaccccgac ttcatcacct tcttccgctc
     2161 ggcctccaag ccgttccaag cgattccgct ggtggcggag ggcggcattg agcgcttcgg
     2221 cctcacttcc caggaattgg ctattatctg cgggtcgcat tccggccagc cggagcacat
     2281 ccaagtcgtt gaacagttgc tgatgaaggt cggtttcatc gaaggagact tgcagtgcgg
     2341 gattcatccc ccatcttttt tcaccgctaa cgaattgact cccgagccgg gagcgcgctt
     2401 cagccaattg caccacaact gctccggaaa gcacaccagc atgctggctc tgtccaaaat
     2461 ctacgacgaa ccgaaagaaa actaccttga tcctaacagt cgcgcgcagc aagcaatctt
     2521 gcaggcggta gccgaggcat gccggtatcc ggcggtagaa ataggcgtcg cgattgatgg
     2581 ctgcagcgca ccgaattttg ctttacctct ccggcatatg gcccgggggt atgctaattt
     2641 cgtcaccgcc cacgggagca ctcccgcctc gagccgggtc taccagacgc tccgtcaggc
     2701 catgctcgaa caccccttga tggtttccgg cgtcgggcgc ttcgactacg ctttgatgca
     2761 agccggacgt gggcaggtgc tgagcaaagc cggggcggaa gcgctggaat gcgctgccct
     2821 cgttgaccgg ggctggggcc tggcggtcaa aattgccgac gggagtcaaa gagccgttgc
     2881 tccggtcgtc atcgaggtgc tgagtcagct gggtgtaccg ctggatatgt cggtgccgaa
     2941 gctggccgag tttgcccgtc ccgtcattaa gaactaccgc ggaagagtag tcgggcaaat
     3001 tgcgccgata ttcaagttgc aggcggtgaa gccagcgaga gtgcgagaag cggttgccgt
     3061 ctgaaatagc ggagagtgat agatgctgat agaaccgtac aaaataaaag tcgttgaacc
     3121 aatccggttg atttcgagag aaaccagcga agcgcggctt aaggaagcgt atttcaacct
     3181 cttcaatctc aaggccgaag acgtctacat cgatcttttg accgactccg gcaccgcggc
     3241 gatgtcggat atccagtggg ccggcctgat gcagggggat gagtcgtacg ccggggcccg
     3301 gagcttctac cggttc
//
LOCUS       DBNGZA010000185         5558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2683683, whole genome shotgun sequence.
ACCESSION   DBNGZA010000185 DBNGZA010000000
VERSION     DBNGZA010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2683683"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="AC16FU_03870"
     CDS             <1..1989
                     /locus_tag="AC16FU_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993542.1"
                     /translation="TCSFLMMGGQGRPPYGLAGIALAQEDPFDQALAKVGLTRETMRF
                     DDDDMANFGGDKFLLPLFTTLHRNPLKIPDYTRVFREAVVQNAGSLENMLTFASLRLK
                     AGVRRNLVANPIAGAVEKSKEPEALYQSILALYAKDGRSLRGEEKAQIQKRCSQVPVE
                     LANLIAVFVYTTVSSKGYLDEAFAEAHDLPMNKIRNQALRLLVADSDLTTPQFERFAE
                     KVDFPYLYAGAEDLAWAVDLISDSLSRLTVLLQHNLTISTPWGVIAVNGTGNDLVTED
                     HPYLLIIDLGGDDTYQTGGATTRSEQPASVLIDLAGNDRYVNSDSLRPAFGAGVFGYG
                     FLVDLGGNDQYESVNNSLGVGLFGFGTLLDKSGNDQYRARTTSQGAGIFGIGLLSDLD
                     GDDRYYACLAAQGFGYTKGCGILVDLRGSDEYVADDSTVVFPSAQTKEHNDSMCQGVG
                     FGRRADITDGHSWAGGLGFLVDAAGDDRYSAGLFAQGCAYWYGVGILADQAGNDTYHG
                     VWYVQGSGAHFGVGILDDVAGNDHYTATMNMAQGAGHDFTVGFLIEEAGDDTYQAPNL
                     SLGGGNDNGIGIFWDKEGNDTYEVSAATTLGRANITAPRGGLRDLMLCLGLFIDSGGN
                     DTYSKDFAKNNTVWTQKGLNTDRPLSAEKGVGIDCEWK"
     gene            2202..2275
                     /locus_tag="AC16FU_03875"
     tRNA            2202..2275
                     /locus_tag="AC16FU_03875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2236..2238,aa:Pro,seq:cgg)
     gene            2324..2788
                     /locus_tag="AC16FU_03880"
     CDS             2324..2788
                     /locus_tag="AC16FU_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HIR2993543.1"
                     /translation="MHTLYIGVIGSGECSKEEYAVAQELGGEIARAKAVLVCGGLSGI
                     MEGAARGAKMHNGTTIGILPGNDRKDANAYIDIAIPTGLGEARNVVLARTADCLIALP
                     GEFGTLTEVAYGLKFGKPVLSLHSWEFTKEIIKVSTPQEAVEAAFHYAGQKR"
     gene            2796..3077
                     /locus_tag="AC16FU_03885"
     CDS             2796..3077
                     /locus_tag="AC16FU_03885"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR2993544.1"
                     /translation="MAELVGARILVKGVVQGVGYRDFVERKAPQYDLKGYVKNLLTGE
                     VEVVVEGEKGLVNDFIGDLRVGPRGARVADVVVHWKPYGKQFDRFGVRW"
     gene            3171..3701
                     /locus_tag="AC16FU_03890"
     CDS             3171..3701
                     /locus_tag="AC16FU_03890"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR2993545.1"
                     /translation="MAAVDLKEKIRNIPDFPKKGVVFRDITPLLQDGAGFQYAVDLFY
                     KKFKDREIERVVGIESRGFIFGGAVAARLGAGFVPVRKLGKLPAETVKKEYMLEYGFD
                     SLEMHLDAIESGQRVLVMDDLLATGGTLAATCGLVEQVGGLVVGVAVLIELSFLKGRA
                     KLKGYDLFSLIQYERE"
     gene            3839..>5558
                     /locus_tag="AC16FU_03895"
     CDS             3839..>5558
                     /locus_tag="AC16FU_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR2993546.1"
                     /translation="MSSNQSVNPPKLPAVMPVLPLMSTTVFPHGVTSIQIGFERNLKL
                     IRDSGGEKSCIVLAGTTVENIDEIKTTDIASLGVATRLLKVAELAAGSLEVTLEGLER
                     VQIEEFVQEQPYFKAKIRPVEEENRFAPETLALVEKVLDKVGKLIELDRRYPGEFYHI
                     FRLNAHDPSRFADVIASTFHLVLGTKLKILETTDVKARLEKLLGYIDDEIMRLELSAK
                     IEAGVKVNLEKIQREYYLRQQLREIKRELGESDPQEEDVERLTRLVGQSSLPQAVQRQ
                     AILEIERLRSLSTASAEYGITRNYVEWLLALPWRADCPTEVDLESLEKTVAETFYGLE
                     KVKTRLLENFSVRKLKPDYRGAVLCFAGAPGTGKTSLAETIARALGRKFVRLNVGGLD
                     DDSDILGERRSYVAAHPGRILEALRESGVCNPVILIDEIDKIGNATTHGDITGALLSV
                     LDPELNRRFLDRYLGFPYDLSPVLFLCATTSAENIPVPIAQKLELIELPGYLEDEKIA
                     IAQKYLLPRLASEVGLEPGSLEVSDNAIRMLIRQYAVEAGLWALQRELETLTRKYARQ
                     RVASGPC"
BASE COUNT         1175 a         1551 c         1674 g         1158 t
ORIGIN      
        1 acgtgctcgt tcttaatgat gggcgggcaa ggacgcccgc cctacgggct tgccggcata
       61 gcgctcgcac aggaggatcc tttcgaccaa gcgctggcca aggtcgggct cacccgcgaa
      121 acgatgcgct tcgacgacga cgacatggcc aatttcggcg gcgacaaatt tcttttgccg
      181 ctcttcacca cgctccaccg caacccgctc aagatccccg attacacccg cgtttttcgg
      241 gaagcggttg tccagaacgc cggttctctg gaaaatatgc tcaccttcgc atccctccgg
      301 ctcaaagccg gggtgcggcg gaatctggtc gccaatccga ttgcgggagc ggtcgaaaaa
      361 tccaaagagc cggaggcgct ctaccagtcg attctggctc tctacgccaa agacggccgc
      421 tccctgcggg gggaagagaa agcccagatt cagaagcgct gttcccaggt gccggtggaa
      481 ctggccaatt tgatcgcagt attcgtctac actaccgtca gctccaaagg ttatctcgac
      541 gaagcctttg ccgaagccca cgatcttccc atgaacaaaa tccgcaatca ggcgttgcgg
      601 cttttggtgg ccgacagcga cctgaccacg ccgcagttcg agcgttttgc cgaaaaggtc
      661 gatttcccct acctctacgc cggggcggaa gacctggcct gggcggtcga tttgatttcg
      721 gattcactct ccagactgac cgtgttgctc cagcataacc tgacgatttc caccccctgg
      781 ggtgtgattg ccgtcaatgg taccggaaac gatctggtta cggaagatca tccttacttg
      841 ctcatcattg acttgggtgg tgacgacacg tatcagaccg gcggcgccac gacccggtct
      901 gagcagcccg cctccgtgct catcgacctc gccggcaatg accggtacgt gaactccgat
      961 tcgctccgcc cggccttcgg tgccggagtt ttcggctacg gttttctggt cgatctgggc
     1021 ggcaacgacc agtacgagag cgtcaataat tccctggggg tcggcctctt tggcttcggg
     1081 acgcttctgg ataaaagcgg caatgaccag tatcgcgccc ggactacttc tcagggggcg
     1141 ggaattttcg gcatcggtct gctctccgat ctggatggcg acgaccgcta ctatgcctgc
     1201 ctggcggcgc agggcttcgg ttataccaaa gggtgtggca tactcgttga tcttcgcggc
     1261 agtgatgaat atgtcgccga tgacagcacc gtcgttttcc cttcggcgca gactaaggag
     1321 cataacgaca gtatgtgcca gggcgtggga tttggccgac gtgcggacat taccgatggc
     1381 cactcctggg ccggcggtct cggatttttg gtggatgcgg ccggcgacga ccgttattcc
     1441 gccggcctct ttgctcaggg ctgtgcctac tggtacggcg tgggtatcct cgccgatcag
     1501 gccgggaacg atacctatca cggtgtctgg tacgttcagg gctccggggc tcatttcggc
     1561 gtcggcattc ttgacgacgt agccggcaat gaccattaca ccgctaccat gaacatggcc
     1621 cagggcgccg ggcacgactt cacggtcggt ttcctcattg aggaagccgg tgacgacacc
     1681 taccaggcgc ccaacctgag tttgggcggc ggcaacgaca acggcatcgg catcttctgg
     1741 gataaagagg gaaacgacac ctacgaggtt tcggccgcca cgaccttggg tcgcgccaac
     1801 atcacggctc cgcgcggcgg cctgcgtgac cttatgctct gtttgggtct tttcattgat
     1861 tcgggcggga atgacaccta ttcgaaagat tttgccaaga acaataccgt ctggacccag
     1921 aaagggctga ataccgatcg tcctttgagt gcggaaaagg gggtcggcat cgactgcgag
     1981 tggaagtgaa atttctgatc aggtgagtaa atgtggtaga ccggctcggg acgagccggt
     2041 caaatcaaaa gtcgggctca gcttgcccga catacttact accacgccga ccagggagac
     2101 gagctcgact acccaggagt caatgtccga ccgttcccct tgaccctgac ccgctgcctt
     2161 ctatattcac gcaggacaag gtgtccactt ttttatacac tcggggcgtg gctcagcctg
     2221 gtagagcact tggttcggga ccaaggggtc gctggttcaa atccagtcgc cccgatgtga
     2281 gacagggatc agggattaga gattagggat cagtaaaacc gcaatgcaca ccctctacat
     2341 cggggttata ggatcgggcg aatgctcaaa ggaagagtat gccgtggcgc aggagttagg
     2401 gggggaaatc gcccgggcca aggcggtttt ggtctgcggc gggctttccg gaataatgga
     2461 aggcgcggcc cggggagcca aaatgcacaa cgggacgacc atcggcattc tccccggcaa
     2521 tgaccgcaaa gatgccaacg cctatatcga catcgccatc cccacggggt tgggagaggc
     2581 ccgcaacgtg gtcttagccc gcactgcgga ctgccttatc gctctgccgg gtgagttcgg
     2641 gacgttgacg gaggtcgcct acggcctgaa gttcggcaag ccggtcttga gtctgcactc
     2701 ctgggagttc acgaaagaga ttatcaaagt ctccacacct caggaagcag tggaggcggc
     2761 tttccactac gcgggccaga aacgctgagg gcaggatggc ggaactggtc ggtgctcgga
     2821 ttctggtcaa gggggtggta cagggcgtgg gctaccggga ttttgtcgaa cgcaaagccc
     2881 cccagtacga tctgaaaggg tatgtgaaga atctcctaac cggggaagtg gaagtggtgg
     2941 tggaagggga gaagggattg gtcaacgact tcatcggcga cttgcgggtg ggaccgcgcg
     3001 gagcccgcgt cgccgacgtc gtcgttcact ggaaaccgta cggaaagcag tttgaccggt
     3061 ttggcgtgcg ctggtgagat tgaaacagtt cgggctcagg atcgcccgga acgggaaacg
     3121 tggcaataac tcacgccaga cgccaagtcg agactcaaaa gaataatctt atggccgctg
     3181 ttgacctaaa agaaaaaatc cggaacattc ccgacttccc caaaaaaggg gtcgtcttcc
     3241 gcgacatcac ccccttgctt caggatgggg cgggttttca atacgcggtc gacctgttct
     3301 ataagaaatt caaggatagg gaaatcgagc gcgtggtggg gattgaatcg cgcggtttca
     3361 tcttcggcgg cgcggtggcg gcccggctgg gagccggatt cgtgccggtg cggaagttgg
     3421 gaaaactccc ggcggagacg gtcaagaaag aatatatgct ggagtacggc tttgattccc
     3481 tggaaatgca tttggacgcc attgagagcg gccagcgggt cctggttatg gacgatcttt
     3541 tggctaccgg cggaaccctg gcggctactt gcggactggt ggaacaggtg ggagggttgg
     3601 ttgtcggcgt cgccgttttg atcgaactct cttttctcaa aggccgcgct aaactcaaag
     3661 ggtacgattt attctccctc attcaatacg agcgggagta gaccacctca acccaattct
     3721 tgcctataat cgctatcttc ttgcagtaca gtaacttgcc gtattctgtt tctattggtt
     3781 atttctttta ttccccggac gatgataaca gtatattgaa tgaagggaat cgactctgat
     3841 gtcatctaat caatctgtca acccgccgaa actgcccgcg gtaatgccgg tactgccgct
     3901 tatgagcacc accgtcttcc cccacggcgt gacgagtatc cagatcggct tcgagcgtaa
     3961 tctgaaactg atccgcgatt ccggcggaga aaagagttgc attgttttgg ccggaacaac
     4021 cgtggagaac attgatgaga taaaaaccac cgatatcgcc tcccttgggg tagcgacccg
     4081 gctgttgaaa gtggccgaac tggcggcagg gtcgctggag gtgaccctcg aaggattgga
     4141 gcgggttcag atcgaagaat ttgttcagga gcagccctac ttcaaagcga aaattcggcc
     4201 ggtcgaagag gaaaaccgct ttgcacccga gactttggct ttagtcgaaa aggtgctgga
     4261 caaggtcggc aagctcattg agctggaccg ccgctacccc ggagaattct accacatttt
     4321 ccggctcaac gcccacgatc cctcgcgctt tgccgacgtc attgcctcca cgtttcactt
     4381 agtcctgggg accaagctca aaattcttga aaccaccgac gttaaagccc gcctggaaaa
     4441 actcttgggg tacattgatg acgaaattat gcgcctggaa ctttcggcga agattgaggc
     4501 cggggtcaag gtcaacttgg agaaaattca gcgggaatac tacctgcgcc agcaactgcg
     4561 ggagatcaag cgcgagctgg gggagagcga cccgcaggaa gaggacgtgg agcggctgac
     4621 gcgtctggtc ggtcaatcgt ccctgccgca ggcagtgcag cgacaggcga ttctggaaat
     4681 tgagcgcctg cgctcgcttt ccactgcctc ggcggagtac ggcattaccc gcaactacgt
     4741 cgagtggctc ctggcgctgc cctggcgggc cgactgtccg acggaggtcg atctggaaag
     4801 tctggaaaag accgtagcgg aaaccttcta tgggttggaa aaggtcaaga cccgtctgct
     4861 ggaaaacttc tcggtccgca agttgaaacc ggactaccgc ggcgcggtcc tctgctttgc
     4921 cggcgcaccc ggcaccggga aaacgtctct ggccgagacc attgcccggg cgctggggcg
     4981 gaaattcgtc cggttgaacg tcggcggttt agatgacgac tctgacattc tgggtgagcg
     5041 ccgctcgtac gtagccgccc acccgggccg catcctggaa gcgctgcggg agtcgggtgt
     5101 ctgcaacccg gtgattctca tcgacgaaat cgacaagatc ggaaacgcca ccacacacgg
     5161 cgacatcacc ggggcactgc tgtcggtcct ggatccggaa ctcaaccggc gctttctgga
     5221 ccgttacctc ggattcccct atgatctttc gcccgtgctt ttcctctgcg cgacgacttc
     5281 cgcggagaat attccggtgc cgatcgcgca gaagctggag ctcatcgaac tccccggcta
     5341 cctggaagac gaaaaaatcg ccatcgcgca gaaatatctg ttgccgcgcc tggcgagcga
     5401 ggtgggactg gagccgggct ctctggaggt gagcgacaac gccatccgga tgttgatccg
     5461 tcagtacgcg gtggaagccg ggctctgggc gctccagcgg gaattggaaa ccttaacccg
     5521 caagtatgca cgccagcggg tggcctccgg tccctgcc
//
LOCUS       DBNGZA010000186         5349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_246175, whole genome shotgun sequence.
ACCESSION   DBNGZA010000186 DBNGZA010000000
VERSION     DBNGZA010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_246175"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2364
                     /locus_tag="AC16FU_03900"
     CDS             <1..2364
                     /locus_tag="AC16FU_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR2993547.1"
                     /translation="EHLLLGLLKLGEGRAIAVLTNIGLDLAELKQGIEDVVQPSGGTM
                     VMGQLPLTARAKKTLEVSGQEARALKSKDIDTEHILLALLKEEEGVAAQVLSMYDVDY
                     KEVYEELKNIQSGKPSAYGRKRKKSKTPALDHFGRDLTELARRSKLDPIIGRDNEIER
                     VSQILSRRKKNNPVLIGEPGVGKTAIVEGMAQRIVEGRVPQTLENKRVVTLDMAALVA
                     GTKYRGQFEERMKAVMNEIINSRDVIIFIDELHTIVGAGGAEGSLDASNIFKPALSRG
                     ELQCIGATTLNEYRKYIEKDGALERRFQTVMVDPPSPGDTIKILEGLKSRYEDHHKVR
                     ITDEAIAASVKLSDRYISGKFQPDKAIDVLDEAGSRAHLASFTKPVTFTDIEQEIARI
                     QRDKEHSVKNQEFERAAQLRDELKFKKDELEKARRAWEEERSKQVVELKAEEIAQVVS
                     KMTGIPVFRLEEVESKKLLRMEEELTKRVVGQSEAINVISKAIRRSRAGLSDPRRPIG
                     SFIFLGPTGCGKTELARALAAFLFEDEEALVRIDMSEYMEKFSVSRLIGAPPGYVGYE
                     EGGQLTEKVRRKPYSVVLLDEIEKAHPEVFNLLLQVLDDGTLTDSFGRKVDFKNTVVI
                     MTSNIGAREIHAGKHLGFVPEEEQFSYERMRQKITEEVKKIFNPEFLNRIDDMVVFHP
                     LGRPEILKIVDIQLKEVAQRLAEKEVSFTLTDAAKEFLADKGFDPVFGARPLKRAIQK
                     YLEDPMAEEILKGQFGTECEVLVDKGEGDGLRFTIAGGRPTLVPVKK"
     gene            complement(3194..>5349)
                     /locus_tag="AC16FU_03905"
     CDS             complement(3194..>5349)
                     /locus_tag="AC16FU_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE2 domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HIR2993548.1"
                     /translation="PWPKATMARFLDALSAQKPKVIAGNFPLPFTAPDTGAVTDSLAD
                     VIKRAKNVILPVSFTLAETGVNTITTPAYITSSAYLIYGEEFDLQDNPPRLAREIVYP
                     EAPLDSAAFGLGHTAFAPDPDGMLRWAPLIVKYEGFYYPSLAVQAARAYYGLVRQQVK
                     IETGKKIWLDNLSIPIDDHGQMLIAFRGPANTFPSVSAADVLSGRVDPSALRDKVAVV
                     GMTATGLTQQYKTAIARGIPRVEVTATVIENIIHRRFYSSLSLSTLLDVGILILIGVF
                     SALILPKVALLYRAVILAVFAFAILNFNFILFASFNLMPKVFYPMMEVFFFLAASFTI
                     KPKKEGAQAEVETGEEEGYAPRQYQRFARQEEAEPPRPERARVEYGPASLELAEMHAT
                     QHMVSSDLATVELEMKTPEQMAAEETQMLVEAKAEAKKQAEAWQLGKLGRYQVLQVLG
                     KGAMGTVYKGLDPALDRLVALKTIRMDFASSPDEVTELRERLLREAKAAGKLSHPNIV
                     TVYDVGQDGDIQYIAMEYLQGYTLEEVIKKKVELNFRIVASIITQICNALEYAHDQGI
                     VHRDIKPANIMVLNGFNIKVMDFGIARIATSNMTQSGVALGTPNYISPEQLTGQEIDR
                     RADIFALGVVMYEFLTRKKPFTGESLSALIYNIINLTPPPPSQINDKVPELFDRIILR
                     ALEKSVLYRYQRAGEIAEDLKTYLASFRSRPVRTY"
BASE COUNT         1131 a         1517 c         1630 g         1071 t
ORIGIN      
        1 gagcacctcc ttttgggctt gttgaagtta ggcgagggtc gggccatagc tgttttgacc
       61 aacatcggcc tcgatctggc cgagttgaag caggggattg aggacgtggt tcagccctcc
      121 ggcgggacca tggtgatggg gcaactgcct ttgaccgccc gggccaagaa gactctggaa
      181 gtctcgggac aggaagcccg cgcgttgaag tcgaaagaca tcgacaccga gcacattctg
      241 ctcgccctct taaaagagga agaaggtgtg gccgcccagg tcctctcgat gtacgacgtc
      301 gattacaagg aagtctacga agaactgaaa aacatccagt cgggcaaacc ctcggcctac
      361 ggccgcaaac gcaagaagag caaaactccg gctttggacc actttggccg cgacttaacc
      421 gaactcgccc gccgcagcaa attagacccg atcatcggcc gcgacaacga aattgagcgg
      481 gtttcgcaaa tcctctcgcg ccgcaagaag aataacccgg ttctgatcgg cgagcccggc
      541 gtcggcaaaa ccgccattgt ggaagggatg gcgcagcgga tcgtcgaagg ccgggtgccg
      601 cagaccctgg agaacaagcg ggtggtcacg ctggacatgg cggccctggt ggccgggacc
      661 aagtaccgcg gccagttcga ggagcggatg aaagcggtga tgaacgaaat catcaattcc
      721 cgggacgtga tcatcttcat cgatgaactg cacaccatcg tcggcgccgg cggggccgaa
      781 gggtccctgg acgcctcgaa catcttcaaa ccggctcttt cccgcggcga actccagtgc
      841 atcggcgcca cgactttgaa cgagtaccgc aagtacattg aaaaagacgg cgccctggag
      901 cggcgcttcc agaccgtgat ggtcgatccc ccctcacccg gcgatacaat caagattctg
      961 gaggggttaa agtcgcgcta cgaagatcac cacaaagtcc gcatcaccga cgaggcgatt
     1021 gcagcctcgg tgaaattgtc cgaccgctac atttcgggaa aatttcagcc cgacaaagcc
     1081 attgacgtcc tggatgaagc cggctcccgg gcccacctgg cctctttcac caaacccgtc
     1141 actttcaccg acatcgaaca ggaaatcgcc cggatccaac gggacaaaga gcattcggtg
     1201 aaaaaccagg agttcgaacg cgccgcccag ctgcgggacg agttgaagtt caaaaaagac
     1261 gaattggaga aggcccgccg ggcctgggag gaagagcgca gcaagcaggt ggtggaactg
     1321 aaggccgaag agatcgccca ggtggtttcg aagatgaccg gcatcccggt cttccggtta
     1381 gaagaggttg agtccaagaa acttttgcgg atggaagagg agttgaccaa gcgggtggtg
     1441 ggccagagcg aagccattaa cgtcatctcc aaagcgatcc gccgctcccg ggccggattg
     1501 tccgacccgc gccgtcccat cggctccttc atcttcctgg gccccacagg ctgcggtaaa
     1561 accgaactcg cccgcgccct ggcggccttc cttttcgaag acgaagaggc tctggtccgc
     1621 atcgatatgt cggagtacat ggagaagttc tccgtctcgc gcttgatcgg cgcgccgccc
     1681 ggctacgtcg gctacgaaga gggggggcaa ctgaccgaaa aagtccggcg caagccttac
     1741 tccgtggtgc ttttggacga aatcgaaaag gcccacccgg aggtcttcaa cctcctgttg
     1801 caggtgttgg acgacggcac cctgaccgac tccttcggcc gcaaggtcga tttcaagaat
     1861 accgtggtca ttatgacctc caacatcggc gcccgcgaga tccacgccgg caagcatctg
     1921 ggcttcgtcc ccgaagagga gcaattctcc tacgaaagga tgcggcagaa gatcaccgaa
     1981 gaggtcaaaa aaatattcaa cccggaattt ttgaaccgga ttgacgatat ggtggttttc
     2041 cacccgctgg ggcggccgga gattttgaag atcgtcgaca tccagttaaa ggaagttgcc
     2101 cagcgcctgg ccgagaagga agtctccttc accctcaccg acgccgccaa ggaattctta
     2161 gccgacaagg ggttcgaccc ggtcttcggc gcccggcccc tcaagcgggc gatacaaaaa
     2221 tacctggaag acccgatggc cgaagagatc ttgaaaggcc agttcggtac ggaatgcgaa
     2281 gtgttagtgg acaaaggcga aggcgacggg ctgcgcttca ccatcgctgg cggccgcccc
     2341 actctcgtgc cggtaaagaa gtaagagatt attcggtttg ggaaaattgt aggggcgggg
     2401 tcaccccgcc cctttttatt ttccctctcc tcgtaggaga gagcaggtcg aagaccccgc
     2461 cagaggcggg ggaggggtca gccctttgtt gcttttcgct tcgcataggg gcgaccgccc
     2521 ccggtcgcct ataattttcc gtagccgagg attttatcct cggtgccccg ctggcggggc
     2581 attgatttga ttttccctct ccttcgaggg gaggggtaca gggagaggtc attcttggta
     2641 gaccggctcg tctctgagcc ggtcgagacg gatgtgtagg ggcgaatcac aattcgccca
     2701 agatttttcc ctctcctgta ggagagggta gggtgaggtc aagatgtagg ggcgaacctc
     2761 gtgttcgccc atctctcaga ggttcttctt ctccctctcc tgtagtggtt cggcccgtac
     2821 gggctcacca ccctgtcttc gaccctgagg gtctctcgtt cagagcctga cggctcttca
     2881 gagccgaagg cagacccgat gggagcttgc cgaaggggag gaggtcgggg tgaggtcaaa
     2941 tccgtagagc tgattcacga attgccccta cccacaatcc gtttaatctc gccagagtcg
     3001 catcgaccgc tccaaaatcc gttgtcccat acgggtcgca ccgacagaat tttggggtca
     3061 catcgggggc acataggtgc cccttccccc gctaatttgg ggttgccttg ggggtaaaac
     3121 ggacaaagtg cgaaataatg ggcaaccttc accccggtgc gaggcgaagg tgatttttcc
     3181 aaatctgaat tttttagtac gtccgcaccg ggcgggagcg gaaggaggcc aggtaggtct
     3241 tcaggtcttc ggcgatttcg cccgcccgct ggtagcggta gaggacgctt ttctccagcg
     3301 cccgcaggat gatccggtcg aacagttcgg gcaccttgtc attgatctgt gagggcggcg
     3361 gcggcgtgag gttgatgatg ttgtagatta aagcggaaag gctttcgccg gtgaagggtt
     3421 tcttccgggt caaaaattcg tacatcacca cccccagagc aaagatgtcg gcccggcggt
     3481 cgatctcctg accggtcaac tgctccgggg aaatgtagtt gggcgtgccc aaggctacgc
     3541 ccgactgggt catattggag gtcgctatgc gcgcgatgcc gaagtccatc accttgatgt
     3601 tgaaaccgtt taggaccatg atgttggccg gcttgatgtc gcggtggacg atcccctggt
     3661 cgtgggcgta ctccagggca ttgcagatct gcgtgatgat cgaggccacg atgcggaagt
     3721 tcagctcgac tttctttttg atcacttcct ccagcgtgta cccctgcagg tactccatgg
     3781 cgatgtactg aatgtcgccg tcctgaccca cgtcgtagac agtgacgatg ttcgggtggg
     3841 agagcttgcc cgcggctttg gcctcgcgta aaagccgttc gcgcagttcg gtcacctcgt
     3901 ccgggctaga ggcaaaatcc atgcggatcg ttttgagcgc caccagacgg tccagcgccg
     3961 gatcgagtcc cttgtagacc gtacccatgg cgcctttgcc caaaacctgc agcacctggt
     4021 agcgtccgag tttgcccagt tgccatgctt cggcctgctt tttggcttcg gccttggcct
     4081 cgactaacat ttgcgtctct tcggccgcca tctgctccgg agttttcatc tccagttcca
     4141 ccgtggcgag gtcggaagag accatgtgct gggtggcgtg catttcagcc agttcaaggg
     4201 aagccggtcc atattcgacc cgcgcccgtt ccggtcgggg cggctcggcc tcttcctgcc
     4261 gtgcgaacct ttggtactgg cgcggtgcgt acccttcctc ttccccagtc tcgacctcgg
     4321 cctgggcacc ttctttcttg ggtttgatcg taaaactggc ggcgagaaag aagaacactt
     4381 ccatcatcgg gtaaaaaacc ttgggcatta agttaaagga ggcgaacaga atgaagttga
     4441 agttgagaat ggcaaaggcg aacaccgcca gaatgaccgc ccggtagagg agggccacct
     4501 tgggcaggat cagcgcggag aagacgccga tgaggatcaa aatacctacg tccaagagcg
     4561 tggagaggga gagactgctg tagaaccgcc ggtgaatgat gttttcaatg accgtggccg
     4621 tgacttccac ccgcgggatg ccgcgcgcga tggcggtttt gtactgctgg gtcaggccgg
     4681 tggcggtcat cccgaccacg gcgaccttgt cgcgcaaagc ggaggggtca acccgtcccg
     4741 agagcacgtc cgccgcggaa acggagggga aggtattcgc cggtccccga aaggcgatga
     4801 gcatctgtcc gtgatcgtca atcggaatcg acaaattatc cagccagatt tttttgcccg
     4861 tctcaatttt cacctgctgg cggaccagcc cgtagtacgc ccgggccgcc tgcaccgcca
     4921 gggaagggta gtagaagcct tcgtacttga cgatgagcgg cgcccagcgc agcatgccgt
     4981 ccgggtcggg ggcgaaagcc gtgtgcccca ggccaaaagc ggcgctgtcc aaaggcgctt
     5041 cggggtagac gatttcccgc gccaagcggg gcggattgtc ctgcagatcg aactcttcac
     5101 catagatgag gtaggcggag ctggtaatgt acgccggcgt ggtgatcgta ttgacccccg
     5161 tctccgcaag ggtgaaactg accggcagga tgacgttctt tgcccgtttg atgacgtccg
     5221 ctaaactgtc ggtaaccgca ccggtgtccg gcgccgtgaa aggcaaaggg aaattaccgg
     5281 cgatcacttt gggcttttgg gccgagagcg catcgaggaa gcgggccatc gtcgctttgg
     5341 gccagggcc
//
LOCUS       DBNGZA010000187         4534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_882655, whole genome shotgun sequence.
ACCESSION   DBNGZA010000187 DBNGZA010000000
VERSION     DBNGZA010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_882655"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..281
                     /locus_tag="AC16FU_03910"
     CDS             69..281
                     /locus_tag="AC16FU_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993549.1"
                     /translation="MKVEKTRGEFYCATVEEAENKKFRRALRYRGLEKKWKKNLALTK
                     KIVKIIIAGFARERLEIAPSAMFVYF"
     gene            339..1469
                     /gene="asnA"
                     /locus_tag="AC16FU_03915"
     CDS             339..1469
                     /gene="asnA"
                     /locus_tag="AC16FU_03915"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR2993550.1"
                     /translation="MTDKRADLAGPGIGSYDELEKILPQDYSSLLNPKDTQKGIFAVK
                     NYIEENLCRELNLMMVQVPLIVDIESGVNDLLDRDGSRTPIQFHISNDYDKHPIDAQV
                     VQAATKWKRVALKQLQMQVGEGLCTDMRAVRKDYFLDHDHSVYVDQWDWEQVITERQR
                     NLEYLKEVVRKIWKVLKGAERHAQKLFPRLKTDRYPDLPEELTFLHAEDILDRFPDLP
                     RKKRETAILQEYPAIFIIGVGWTLKDGYPHEMRAADYDDWVTETVSEDGRPMHGLNGD
                     ILVWNPVTRRRHELTSMGIRVNAVTLKKQLEITGLLDFLKFPYHQAIINNEIPLSIGG
                     GIGQSRTYMLLLKKAHLGEVSVTVWPKILKELCRKRNIFVLE"
     gene            1635..2564
                     /locus_tag="AC16FU_03920"
     CDS             1635..2564
                     /locus_tag="AC16FU_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993551.1"
                     /translation="MAEKQTGKVIQVEHLRKVYGSVIAVDDISFEVYQGEIFGMVGPN
                     GAGKTTTIECVEGLRKPDQGHIRVLELDPQKNGYALRERMGVQLQEAVLPERMKVWEA
                     LDLYAAFYPRHTDWESLLTRLGLAERRSAHFAKLSGGQKQRLFIALALIHDPEVVFLD
                     ELTTGLDPHARRALWDLVREVRDQGKTIFLTTHFMEEAERLCDRVAIVDHGRIVALDS
                     PENLIRRLGVENRVVFSVDGSFNSEQLSHLAEITKVERIGERVIVYGRGDSLVRVVVD
                     SLSANGFRFRDLRTEQPTLEDVFLSLTGRELRE"
     gene            2575..3318
                     /locus_tag="AC16FU_03925"
     CDS             2575..3318
                     /locus_tag="AC16FU_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993552.1"
                     /translation="MRGVWKLTWMEMKLFLRQPAAAFFTLAFPQMLLLLFGSVNGNKP
                     TPFFGGLGFVDVSVPAYTAIIIATTGLMSLTIHMAAYREFGILRRLKATPLVPQAILF
                     AQVVVLFAMTSLGMAVLVLVGKVVYGLRFSGNPLSVAAAFVLSSISFFSLGFVLAGFV
                     PNARTAQIVAMVLFYPMIFLSGATFPREVLPETIRHYSQILPLTHVVTLLRGLWVGDS
                     WGKHLPEVGILAGCIVVGVIVSAKTFRWE"
     gene            3497..3943
                     /locus_tag="AC16FU_03930"
     CDS             3497..3943
                     /locus_tag="AC16FU_03930"
                     /inference="COORDINATES: protein motif:HMM:NF018942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR2993553.1"
                     /translation="MEVSRAWALLFARCVLGLIFFMAGWYKVFTMGAPEHARRLFVEP
                     YANTFLPVWSLWATGTAIPYVELVAGALLIIGLRVREASVALGVVLVVVTFGHLLKEP
                     FYEFHTHVIPRLALLLFILLLPRQADRFSLDHLLARGANLPAEPGR"
     gene            complement(4031..>4534)
                     /locus_tag="AC16FU_03935"
     CDS             complement(4031..>4534)
                     /locus_tag="AC16FU_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993554.1"
                     /translation="TLSKKYGIINQYTSFLVKVDPAAPLAQTSLQFMQEEAAPLRAEA
                     GREAVAASKVARESKDMATISGSFDALHRRGEDGAVNLATAGGKTFFRQNEKWVESDR
                     DDKKPVLKIQRFSEAYFALLRIRPEFGKYLALGENVSFDAGKVTVEIAADGKEKLTET
                     ELNQYFK"
BASE COUNT          967 a         1210 c         1312 g         1045 t
ORIGIN      
        1 accgtgaagg tttctagaat tgacagaaca agatttatca cctgcccttt gaccaacagt
       61 acgataatgt gaaagttgaa aaaacaagag gagaatttta ttgtgctacg gtggaggaag
      121 cagaaaacaa aaaattccgt agggctctta gatatagagg tttagaaaag aagtggaaaa
      181 agaatttggc gctaacgaag aaaatcgtta aaattatcat agcgggcttc gcccgcgaaa
      241 gacttgaaat tgccccttcg gcgatgtttg tttactttta ggttgtgctg gcggttcctc
      301 ttatgaaacg ctgaaaattg caaccggagg tagatagcat gacagacaaa agagcagacc
      361 tggcggggcc gggcatcggc agctatgacg aactggagaa aatcctgccg caggactata
      421 gctcgctttt gaaccccaaa gacacccaaa aggggatctt tgcggtaaag aactacatcg
      481 aggagaattt gtgccgggaa ctcaacctga tgatggtaca ggtgcccctg atcgtggaca
      541 ttgagagcgg cgtgaatgac ctcctcgacc gggacgggtc ccgcaccccc atccagttcc
      601 acatttccaa cgattatgat aagcacccca ttgacgccca ggtggtgcag gccgcgacca
      661 aatggaaacg ggtggcgctg aaacagctgc agatgcaggt cggcgagggc ctgtgcaccg
      721 acatgcgcgc ggtgcgcaaa gactacttcc tcgaccacga tcacagcgtc tacgtggacc
      781 agtgggattg ggagcaagtg attacggaaa ggcagcggaa cctggagtac ctgaaggaag
      841 tagtccgaaa aatctggaag gttctcaaag gcgccgaacg gcacgcccaa aaacttttcc
      901 cccggctgaa aacagacagg tatcccgacc tgccggagga attgacgttc ctccatgccg
      961 aagatatcct ggatcggttc cccgacctgc cgcgcaaaaa gcgcgagacg gcgatcctgc
     1021 aggaataccc cgcaattttc atcattggcg tcggctggac gctcaaggac ggctaccccc
     1081 acgaaatgcg ggcggctgat tacgatgact gggtcaccga aacagtgtcc gaggacggcc
     1141 ggcccatgca cggactcaac ggagatatcc tggtctggaa cccggtcacc cggcgccgcc
     1201 acgaactgac ctccatgggt atccgcgtga acgctgtgac actcaagaag cagctggaga
     1261 ttaccggcct gctcgatttt ctcaaattcc cctaccacca ggcaataatc aataacgaga
     1321 ttccgctcag cattggtggc ggcatcgggc agtcccgcac ctacatgctc ttgctcaaga
     1381 aggcgcactt aggcgaggtc agcgttaccg tgtggccgaa gatactgaag gagttgtgcc
     1441 ggaagagaaa tatatttgtt ctggagtgaa cggaaatcgg gccgataatg ctcctctccg
     1501 agcgcagctt acgcaaaccc ttctgtccgc agtgaaaatc gcaggtaagg tacttatatg
     1561 atttgccata aatcgtaact ctggggaaga attaacgtag gtattaatca ggactgacgg
     1621 gtgattctac attgatggca gagaagcaaa cgggaaaagt cattcaggtt gaacacctgc
     1681 gcaaagtcta cggttcagtc attgccgtag atgacatttc gtttgaggtg taccaggggg
     1741 aaatcttcgg gatggtgggg ccgaatggag ccggcaaaac cacgactatt gagtgtgtcg
     1801 aaggcctgcg gaagccggac cagggacaca ttcgtgtcct ggaattggat ccccagaaaa
     1861 acggttatgc actccgcgag cggatggggg tgcagttgca ggaggcggtg ctccccgaac
     1921 gcatgaaagt ctgggaggcg ctggatctct atgccgcatt ttacccccgc cataccgact
     1981 gggagagttt gctgacgcgg ctggggctgg cggaacggcg cagcgctcac tttgccaaac
     2041 tttccggcgg gcagaaacag cgccttttca ttgcgctggc actcatccat gacccggaag
     2101 tggttttcct ggacgaattg accaccggtc tcgatcccca cgcccgccgg gccttgtggg
     2161 atttggtgcg ggaagtccgt gaccagggaa agacgatttt tctcaccaca cactttatgg
     2221 aagaagccga acggctctgt gatcgtgtcg cgatagtaga ccacggccgc attgtcgccc
     2281 tggactcgcc ggagaacctg atccgcaggc tgggtgtgga gaaccgggtg gtgttcagtg
     2341 tcgatggaag cttcaattcg gaacaactca gccatttagc cgagataacc aaggtcgaga
     2401 gaatcgggga acgcgtcatt gtctacggac gcggcgatag cctggtcagg gtcgtggtcg
     2461 attctctctc cgcaaacggt ttccgttttc gcgacctgcg taccgagcag ccgactctgg
     2521 aggatgtttt tctgtcactc accggccggg aattacgaga gtaacggagc aactatgcgt
     2581 ggtgtctgga aactgacctg gatggaaatg aagctcttcc tgcgccagcc ggcggcggcg
     2641 ttcttcactc tggcctttcc gcagatgttg ctgctcctct tcggcagtgt caacggcaac
     2701 aaaccgacgc ccttcttcgg cggactcggc tttgtggatg tttcggtccc ggcttacacg
     2761 gccattatca tcgccaccac cgggcttatg tccctgacca ttcacatggc ggcctatcgc
     2821 gaattcggga ttctgcgccg cttaaaggcc actcccttgg tcccgcaggc catccttttc
     2881 gcccaggtag tggtgctctt cgcgatgacc tccctgggga tggcggtgct tgttctcgtg
     2941 ggcaaggtag tctacggttt gcgcttctcc ggcaatccct tgagtgtcgc ggccgctttt
     3001 gtgctcagca gcatcagttt cttctccctg ggttttgttt tagccggttt tgtaccgaat
     3061 gcccgcaccg cacagattgt ggcgatggtg ctcttctacc ccatgatctt tctttcggga
     3121 gcgacgtttc ctagggaagt tttgccggaa accattcgcc attattccca gattttgccc
     3181 ctcacgcatg tggtgacctt gctgcggggg ctgtgggtcg gtgacagctg ggggaagcat
     3241 ttgccggagg tgggcatcct ggcgggatgc atagttgtgg gagtgattgt gtcggccaag
     3301 acgttccggt gggagtgata gaacagggat gttcgctggt ggggtagggt cgtaaccgag
     3361 gaaggaggga aaaccgatgt tcgcaggaat caattttctg gcggtactcg tggcggccgg
     3421 gttgtacgca ataaataatg gctatcacct gctttctttt ctcatcatgg gagtcattct
     3481 ggtggtctgg cgataaatgg aggtatcccg cgcctgggcg ctgctttttg cccgctgcgt
     3541 tctgggcctg attttcttca tggcgggctg gtacaaagtc tttactatgg gtgcgccgga
     3601 gcatgcgcgc cgcttgtttg tggagccgta tgcgaacact ttcctgccgg tctggtcgct
     3661 ctgggccacc ggcacggcca ttccgtacgt cgaactcgtg gcgggggcgc tgctcattat
     3721 cggcctgcgc gtcagagagg cgtcagttgc gctcggagtg gtactcgtgg ttgtgacctt
     3781 cggccacctt ttgaaagagc cattctacga gttccacacc cacgtcatcc cgcgcctggc
     3841 cttgctcctc ttcattctcc ttttgccccg gcaggccgac cgcttttctc tcgatcacct
     3901 cttggcccgt ggggcgaacc ttccagccga gccgggacga tagcggggcg agcgaaggga
     3961 atcagtcata tatccataga aaaaccggcc ctaactgcgg gccggctttc gtttccagta
     4021 gctcctactg ctacttgaaa tactggttca attcggtctc agtgagtttc tctttgccgt
     4081 ccgcggcgat ttccaccgtc actttgccgg cgtcaaaact gacgttttcg cccagggcca
     4141 gatatttgcc gaactccggc cggattctca acagcgcaaa gtaggcctcc gaaaagcgct
     4201 ggattttgag gaccggcttc ttgtcgtccc ggtcggactc cacccatttc tcgttttgcc
     4261 ggaagaaggt tttgccaccg gcggtggcaa ggttgacggc tccgtcttca cccctcctgt
     4321 ggagggcatc aaagctgccg ctgatggtgg ccatgtcttt actctctctc gcaactttac
     4381 tggcggccac cgcctcccgc cccgcttcgg cacgcagtgg tgcggcttcc tcctgcataa
     4441 attggaggct ggtttgcgcc aaaggcgcag ccggatcgac cttgaccaaa aacgaggtgt
     4501 actggttgat gatgccatat tttttggata gtgt
//
LOCUS       DBNGZA010000188         2971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2578286, whole genome shotgun sequence.
ACCESSION   DBNGZA010000188 DBNGZA010000000
VERSION     DBNGZA010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2578286"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..864
                     /locus_tag="AC16FU_03940"
     CDS             301..864
                     /locus_tag="AC16FU_03940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2993555.1"
                     /translation="MTKPQGPFFTLPNLLSLSRLLLVPVFILLAFQEGASVRWLLAAV
                     IIYGNTSDIADGLIARYTKQVSDWGKILDPLSDKISTATVAWYVWLRHDFPLWAFILL
                     LVKDIAILVAGTVTISKSTAIPVSNFWGKTAALLWAVTVLAYFLDLRSMKEGFLYAAT
                     AATIPALVSYGKRWQRQKKEALAGVIR"
     gene            819..2588
                     /gene="recN"
                     /locus_tag="AC16FU_03945"
     CDS             819..2588
                     /gene="recN"
                     /locus_tag="AC16FU_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR2993556.1"
                     /translation="MAASEEGSAGGSDTLMLRRLAIKNYAIIDDVTVEFTPSFTVLTG
                     ATGAGKSIIIGALNLLLGEKASSEVVRSGETNAWVEGEFEIERSEPVTSLFSEAGIET
                     DATLLIRREVADRGTSRALVNDRQVTVGFLKRLGDFLVDLHGQHQHQWLLAAERHLDF
                     LDGFCGTLELREKMGELYNSYVTAHRKLRDLEKLQKESGEREELFRFQVTEIRKANLS
                     IAEEETFLAEKKRLDHAQELVQSAERLYDSLYDSEGSATERLKLAVRELSRLGAIDAD
                     LAKQAESLEQAHIVTQDVASFLGQYRERVGFDPERYQQVVDRLDLYFRLKKKYGGSVD
                     AVNALADDLEKQLHQRMNLGEEIKQTTAELARLRGECARVATALSTKRTEGAGALAKR
                     VEKELEQLGMAKTKFQVQIVQTQADDGVAVNGKTWALSETGIDQVEFLIAPNVGETLK
                     PLAKIASGGELSRIMLALKSVASRADQVDTLVFDEIDVGIGGEVAGAVGRKLKALAKT
                     KQVLCITHLQQIAALADHHLLVSKEKEKNRMVTRIKPLSPKERVPEIARMLSGEKPTA
                     LTLEHAEEMLKTAVRLAHRPEQS"
     gene            2625..>2971
                     /locus_tag="AC16FU_03950"
     CDS             2625..>2971
                     /locus_tag="AC16FU_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR2993557.1"
                     /translation="MRREFKPKFDRWLVALIFGPFLVIGVVLVIVSWASTKTQPAQAG
                     QLLMPLLLIAGIALLLITLIRSTRYILTASDLVVKLGPIHFKFPYGTIQSVEAGGAEK
                     LWSRGGALRMAFS"
BASE COUNT          668 a          860 c          827 g          616 t
ORIGIN      
        1 cggcaatcgg gcggccgtcg tcggagaccg ccacggctcc tgccgccacc aaatcgccga
       61 tttcggtcaa ctgtttactt tccaactgct tggttgcggc cccgatagga tagacgcggc
      121 atttgactaa ctgcgcccgc tcgtagatga atttaatcga ctcacgaaat aattgttttg
      181 taggggctca atgcgttgag ccctccaatt tcgatttttc aaatatattg agcccccgta
      241 ccttgtcaaa atcgtgcgcc tgctgtagat ttctggcact cggacgggtc tccgagtcct
      301 atgaccaaac cacaaggacc gtttttcact ttgcccaatt tattgtccct ctcgcggctt
      361 ttgctcgtgc cggttttcat tcttctggct tttcaggaag gcgcatcggt tcgctggctt
      421 ctggcggccg ttatcatcta cggcaacacg agcgacatcg ccgacggcct gattgctcgc
      481 tacaccaagc aagtttccga ctggggcaaa attttggatc cgctttcgga caaaatcagc
      541 accgccaccg tggcctggta cgtctggctc cgccacgatt tccccctctg ggcgttcatt
      601 ctgctattag tcaaggacat tgctatttta gttgctggaa cggtcacaat ctctaagagc
      661 actgcgattc cggtctccaa tttctgggga aaaaccgcag cgctcttatg ggcggtgacg
      721 gtgctggcct acttcctcga tttgcggtcg atgaaagaag gattcctcta tgccgccacc
      781 gccgcaacca tccccgcgct cgtttcgtac gggaaaaggt ggcagcgtca gaagaaggaa
      841 gcgctggcgg gagtgatacg ctgatgctcc gccggctggc gataaaaaat tacgccatca
      901 tcgacgacgt gacggttgaa ttcacgccca gcttcaccgt gctcaccggc gccaccggcg
      961 cagggaagtc tatcataatc ggtgccctca atctcctgtt aggggagaag gcgagttccg
     1021 aagtcgtccg ctccggcgaa acaaacgcct gggtggaagg cgaattcgaa atcgagcgct
     1081 ccgagccggt cacgtcgctt ttttctgagg ccggcatcga aaccgatgca acccttctca
     1141 tccggcgcga agtggccgac cggggcacca gccgcgctct ggtcaacgac cgacaggtaa
     1201 ccgtcggctt tcttaaacgt ctcggagatt tcctcgttga cctacacggc cagcaccagc
     1261 accagtggct tctggctgcc gagcgccacc tcgacttcct cgacggcttc tgcggcactc
     1321 tcgaactacg cgaaaaaatg ggcgaattat acaactccta tgtcaccgcc catcgaaaac
     1381 taagagattt ggaaaagctc cagaaggaga gtggagaaag agaggaactt ttcagatttc
     1441 aggtcaccga aatcagaaaa gcgaatctct ccattgccga agaagaaaca ttcctggccg
     1501 aaaagaaacg cctcgaccac gcgcaggaat tagttcaatc tgccgaacgg ctctatgact
     1561 cgctctacga ctccgagggc tcggccaccg aacgcctgaa actcgcggtg cgggaattga
     1621 gccgcctcgg agcaattgac gccgacctgg ccaaacaggc cgagtctctg gagcaggcgc
     1681 acatcgttac acaggatgtg gcgtcatttt tgggccagta ccgcgagcgg gtcggcttcg
     1741 atccggaacg ctatcagcag gtggtggatc ggttggacct ctatttccgg ctgaagaaga
     1801 aatacggcgg gtcggtggat gcggtgaacg ctctggcaga cgatctggaa aaacagttac
     1861 accagcggat gaatctcgga gaagaaatca aacaaactac ggcagaactc gcgcgcctgc
     1921 gcggcgagtg tgcgcgggtg gcgacggcac tctcaaccaa acgcaccgaa ggcgccggcg
     1981 ctctggccaa acgcgtggag aaagagttag agcagttggg aatggcgaag acgaagtttc
     2041 aggttcagat cgtgcagacc caagcggacg atggggtagc ggtcaatggc aaaacttggg
     2101 ctttatcgga aaccggcatc gatcaagtag aattcttgat tgctcccaat gtcggagaaa
     2161 cactcaagcc cctggcaaag attgcctccg gcggcgaact ctcccgcatt atgctggctt
     2221 tgaagtcggt tgcctcccgg gctgatcagg tcgatacgct ggtgttcgat gaaattgacg
     2281 tcggcatcgg cggggaagtg gccggcgccg tggggcgcaa gctgaaagcg ttggcgaaaa
     2341 ccaagcaagt gctctgcatc acccatctcc agcaaattgc agccttggcg gatcaccatt
     2401 tgctcgtttc caaagaaaag gagaaaaacc ggatggtgac gcggatcaag ccgctgagcc
     2461 cgaaagaacg ggtgccggaa atcgcccgga tgctctccgg cgaaaagccg actgccctga
     2521 ccctcgagca cgccgaagaa atgctcaaaa cagcggttcg actggctcac cgccccgaac
     2581 aaagctgagg ggcggcgcgt ccttaagcaa accggaactc agatatgcgc cgtgaattca
     2641 aaccgaaatt cgaccgctgg ctcgtggcct tgatcttcgg cccgtttctc gtgattgggg
     2701 ttgttctggt aattgtttcc tgggccagta ccaagaccca gcccgcccag gccggacagc
     2761 tgctcatgcc gctgctcctc attgccggga tcgctttact cctgataacc ctcatccgct
     2821 ccacgcgcta catcctgacc gccagcgatc tggtcgtgaa gctcgggccg atccacttca
     2881 aattcccgta cggcaccatc caatccgtgg aggccggcgg cgcggaaaaa ctctggagcc
     2941 gcggcggagc gctgcggatg gccttttcga a
//
LOCUS       DBNGZA010000189         4374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1836937, whole genome shotgun sequence.
ACCESSION   DBNGZA010000189 DBNGZA010000000
VERSION     DBNGZA010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1836937"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..652)
                     /locus_tag="AC16FU_03955"
     CDS             complement(47..652)
                     /locus_tag="AC16FU_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993558.1"
                     /translation="MMNLEVNQAVMVTVELDFGGKPPAIAEALRGIERISEPFDGVGR
                     TFAVIDAYGEPTAEGKLHLSMHVSTEKPGRGSLVFRRTGEILWQCEMRPSPNPNAAFT
                     GKNLSIFVDDGKGKSLTVVGANNPSSILTANVRELGRPVASIWPDGAEREVTFIYSAC
                     GCPVKVMVKRVGDRTVRTKELPVIFPDDPAAVTTIEQLMRW"
     gene            complement(1062..1520)
                     /locus_tag="AC16FU_03960"
     CDS             complement(1062..1520)
                     /locus_tag="AC16FU_03960"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2993559.1"
                     /translation="MTEVERIADQLNRVFEGDAWHGPSVREVLKDVSAEKAAKKPIPN
                     AHSIWELVLHIAAWENAVRRRIEGDNVDLTDEEDYPPIRDTSESAWQKAMKMLENGHR
                     ALRKVVSGLTDSRLEELIRGRNYSVYYLLHGIIQHDIYHVGQIALLKKAG"
     gene            complement(1539..1964)
                     /locus_tag="AC16FU_03965"
     CDS             complement(1539..1964)
                     /locus_tag="AC16FU_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR2993560.1"
                     /translation="MKRKASAVWKGGLKDGKGTVSTESGVLSNTQYSFGGRFESGKGT
                     NPEELVAAAHAGCFSMALSAELGKANLTPQSINTAATLTLDMLEGGPTITAIHLETTV
                     RIPGANKAAFEKAANDAKAGCPISRLLNTKITMDAKLEA"
     gene            2252..3814
                     /locus_tag="AC16FU_03970"
     CDS             2252..3814
                     /locus_tag="AC16FU_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR2993561.1"
                     /translation="MPVLSVIFRRKKSLIIRLLTWLLVSGYWLSFFGRAAATPLEDIP
                     LDSWVYPALEELYSRGYSYEFFLSDKPYSRGEVARCLKKISASNPHLLENDFLILRLR
                     YEFADELAALENPPETDKPLEARGGAFVRGNGITSGKARPIQRGAVGVFLGLEAEDKF
                     VTRARMTLDTRTQDDPAIRGRRWHNSVTLFFDDAYAKVHTRYLDLFWGRQHLQWGVGS
                     DALLLSNHFPPLEQVKLKANYKTLRYQFLASRLDPIFIDTLGGNVPRFFSAHRLSWKP
                     WPWLEAGVSESIVYGGPGRSFELFYLNPLLPFYAEQYNNFVDDNPFVGVDFLFSRIQH
                     LVFFGELMIDDFQIDFKSEPQQIGFRLGGWISDFISKGRTFGQLEYTRVNTFVYGQNK
                     PWNIYTRFGAGMGSEIGPDADRWRGSFLWRAHRWVDLVLSGEYRRRGENRITTPQNVA
                     VPYEKFPSGVVEKTALASFTGRLQYQANVFADLTCGGTFLRNGDNRRGNNRDDFFVRG
                     TFTITFWKTKQY"
     gene            3814..>4374
                     /gene="ispE"
                     /locus_tag="AC16FU_03975"
     CDS             3814..>4374
                     /gene="ispE"
                     /locus_tag="AC16FU_03975"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR2993562.1"
                     /translation="MGNQSLVSLMQHSQETQYAAFALKSFAKVNLSLSILGQRADGYH
                     DLASLVQAIDLHDTLEFSPRPSGILVQTDQPELQGSDDNLIYQALQKLSQESRITSGV
                     QVKLTKRIPLGSGLGGGSSNAATALWGGAQLWDLAKPVTELSRLGAEIGSDVPFFFSS
                     GQAVIRGRGEVVEPLRLFADYRVVVVY"
BASE COUNT          844 a         1219 c         1203 g         1108 t
ORIGIN      
        1 gttctggtga cggtcacggc caaatcctca tccggcggtt ctgcatttac cagcgcatca
       61 gctgctcgat ggtggtgacc gctgccgggt cgtcgggaaa gatgacgggc agttctttgg
      121 tgcgcaccgt ccggtctccc acacgcttca ccattacctt cacggggcag ccgcaggcgc
      181 tgtagatgaa cgtcacttcg cgctcggcgc catcgggcca gatggatgcc accggccggc
      241 ccaattcccg aacgttggcc gtcagtatgg agctgggatt gttggcaccg acgacggtca
      301 acgacttgcc cttgccatcg tccacgaaaa tcgaaagatt tttgccggtg aacgccgcat
      361 tcgggttcgg ggaaggcctc atctcgcact gccacagaat ttctccagtg cgccggaaga
      421 ccagcgagcc cctccccggc ttttccgtgc tcacgtgcat ggagaggtgc agcttcccct
      481 ccgcggtggg ttctccgtag gcgtcgataa ccgcaaacgt ccgccccact ccatcgaaag
      541 gctccgagat gcgttcaatg ccgcgcagcg cttcggcgat agcgggcggc ttccctccga
      601 agtcgagctc cacggtgacc atcaccgcct ggttcacttc caaattcatc aattggggtg
      661 ccgggttaag ttttggcttt tgatccggtt ggcccggcag cgtgacggct tccgggtttt
      721 tgtccgtcgc cagcggaccg gttgtttctt tcgagggttt cgctacccgg tttcttcttg
      781 ctgtgaataa gaagatgctc ccaactatca ccattgttag aataattgtg gcggtgagcc
      841 gccgtttgaa ttttgaagga gaagccgggt gaatcgttgg ctctgctccg cccgggtaat
      901 tttttttctt gtttttgggc atggaactat ttaccctcag gctgtcccac catccgccgt
      961 aggaagaacg gtaaatcgtt tcgtctgcct caggctcgat tttttccgca ccgccgggtc
     1021 aacggttttt cccgaactgt gcgtgtgccc ttttctcccg gtcaccctgc ttttttcaac
     1081 agcgcgattt gaccgacatg gtagatgtca tgctggatga tgccgtggag cagatagtaa
     1141 acgctgtaat tccggccacg tatgagctcc tccaaccggg aatcggtgag tccggagact
     1201 acttttcgca gtgcccggtg gccattctcc agcattttca ttgctttttg ccaagccgac
     1261 tcactggtgt ctcttatagg aggataatct tcctcgtcgg taaggtcgac gttgtctccc
     1321 tctattcttc tgcgcacagc gttttcccag gcggcaatgt gcaggacaag ttcccagatg
     1381 ctgtgagcat ttgggatggg ttttttagct gctttttcag ccgatacgtc ttttaaaact
     1441 tctcgaaccg aaggcccgtg ccaggcatcg ccctcaaaaa cacggttcaa ctgatccgct
     1501 attcgttcga cttcagtcac aagtcctccg gattttggtc acgcttccag tttggcatcc
     1561 attgtaatct ttgtgtttaa caggcgcgag atggggcagc cggctttggc gtcgttggcg
     1621 gctttttcaa aagcggcctt gtttgcgccc ggaattctga cggttgtttc taaatggatt
     1681 gcggttatgg ttggaccgcc ttccagcata tccagagtaa gtgtcgcagc cgtgttgatg
     1741 ctctgcgggg tcagattggc tttgccgagt tcggcggaaa gcgccatcga aaagcagccg
     1801 gcgtgggccg cggcgactaa ttcttcggga ttagtgcctt tcccgctttc aaagcggccg
     1861 ccgaaggagt actgggtgtt cgagaggaca ccgctttcgg tcgagaccgt tcctttgccg
     1921 tcttttagac cgcctttcca gactgcggat gctttgcgtt tcatttctcc tcctttacct
     1981 ttagtcttcg gttgagatcg ttttgccgat ttcagcttaa tacggcctct tcaatcgcgt
     2041 cgatcttgcc ttaggtgcaa attctctcaa ttccttaacc cgattgggag aatataatta
     2101 cttatatcaa gaccgacagc actctttgga aaaagacttt aaggcgggat ggtttaactc
     2161 aaggtaggca catcggctga ggttcggcat tgaatcagaa tttcttgaca ataaaatgct
     2221 ttggtgtcat tttgggtctg cctgaaataa cgtgcccgta ctttctgtga tctttagacg
     2281 caagaagagc cttatcatac gattattaac ctggctactg gtctctggct actggctttc
     2341 cttctttggc cgggccgcag ccacgccgct ggaggacatc ccgctggaca gctgggttta
     2401 tccggcgttg gaagaattat acagccgagg gtactcttac gagttttttt tgtccgacaa
     2461 gccctatagc cggggcgaag tggccagatg tctcaagaaa atatccgcct cgaatcccca
     2521 cctccttgag aatgattttc tcatactccg cctgcgctat gagttcgccg atgaactggc
     2581 cgcactcgaa aaccctcccg aaacagacaa accgcttgaa gcgcggggcg gcgcttttgt
     2641 ccggggtaat gggataacct ctgggaaagc cagacccatc cagcgcggag cggttggcgt
     2701 ctttttagga ttggaagcgg aggacaagtt cgtgacccgc gcccggatga ccctcgacac
     2761 ccgcacgcag gacgacccgg ccatccgcgg ccgccggtgg cacaacagcg tgactttgtt
     2821 cttcgacgat gcctacgcca aggttcacac ccgctacctt gacttattct ggggccggca
     2881 acacctgcag tggggagtgg ggagcgacgc gcttttgctg tcgaatcatt ttccgccctt
     2941 ggagcaggtc aaactgaaag ccaactacaa gaccctgcgc taccagtttt tggcttcgcg
     3001 gttggatccg attttcattg acactctggg agggaacgtt ccgcgcttct tttcggcgca
     3061 ccggctctcc tggaaaccct ggccgtggct ggaggcgggg gtttccgaga gcattgtcta
     3121 cggcgggccg ggccgcagtt ttgagctctt ctacctcaat ccgctcctgc cgttctacgc
     3181 tgaacagtac aataatttcg tggacgacaa ccccttcgtg ggcgtcgatt ttctcttctc
     3241 gcgcatccag cacctcgtct tcttcgggga attgatgatt gacgacttcc agatcgattt
     3301 caaaagcgag ccgcagcaga tcgggttccg cctgggcgga tggatcagcg attttataag
     3361 taaaggccgc accttcggcc agttagaata cacgcgggta aataccttcg tctacggaca
     3421 gaacaaaccc tggaacatct acacccgttt cggcgccggg atgggaagtg aaattggccc
     3481 cgatgccgac cgctggcggg gaagtttcct ctggcgagcc catcggtggg tcgatctcgt
     3541 cctttctgga gagtaccgcc ggcgcgggga aaatcgtatt accacaccgc agaacgtggc
     3601 ggtgccgtat gaaaaattcc cctccggagt cgtcgaaaaa accgccctgg cgtccttcac
     3661 cgggcggctg cagtatcaag caaatgtttt cgccgacctc acctgcggcg gtacatttct
     3721 gcggaacggc gacaaccgcc ggggaaataa tcgggatgat tttttcgtcc ggggaacgtt
     3781 taccatcacc ttctggaaaa cgaagcagta ctgatgggca accagagttt agtctctctc
     3841 atgcagcaca gtcaggaaac gcaatacgcc gcctttgccc tgaagagttt tgcgaaggtc
     3901 aatctctccc tctccattct gggccagcgc gccgacggct accacgacct cgcctcgctg
     3961 gtgcaggcga ttgacctcca tgacacgctg gaattttccc ctcggccttc gggtattctt
     4021 gttcaaacgg atcagccgga gctgcagggg tccgatgaca atttgattta ccaggcattg
     4081 caaaagctct ctcaggaatc gagaataacg tcgggagttc aggttaaact gaccaagcgg
     4141 attccactgg gttccgggct gggcggcggg agttccaatg cggctacggc gctctggggt
     4201 ggggcgcaac tctgggatct cgctaagccg gtcaccgaat tgtcacgctt gggagcggaa
     4261 attggctcag acgtaccttt tttcttcagt tccggccagg cggtcattcg cgggcggggt
     4321 gaagtggttg agcccttgcg actttttgcg gattaccggg tagtcgtcgt ttac
//
LOCUS       DBNGZA010000190         4725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_564251, whole genome shotgun sequence.
ACCESSION   DBNGZA010000190 DBNGZA010000000
VERSION     DBNGZA010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_564251"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..3123
                     /locus_tag="AC16FU_03980"
     CDS             247..3123
                     /locus_tag="AC16FU_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2993563.1"
                     /translation="MRQRTVFLTVIGFLLLPTGVFAFQPLFDTWLAYDVGSIQKGIFA
                     ADVDGSGGLDLLVADYGTNTVSVLLNNGDKTFAPKVNYATGTRPDAVFAADLDGDGDQ
                     DLAVVNYGSRTVSILKNNGNGTFAVKVDYPTGNGPKSIYIADLDGDGDRDLAVANDSS
                     NTVSILKNNGNATFAAKVDYAAGTNPASVFGANLDGDGDIDLAVANAGSDNVSILLNN
                     GDGTFAAKVDYSSPGNPFSIYAADLDGDGDRDLAVASGFVSGSGFRIFKNNGNGTFAA
                     GVGYSVPTGGGGGLSSVVLTDVDGDGDQDLVVANPATSMVHVGKNDGTGTFFSGPTIW
                     ADYPSGPGPLALFAADLDGDTYPDLAAANGGTNSISVHRNDGNGAFDRRQDINVGSGG
                     HSVYAADLDGDLDRDLAVESGYGFAIYKNGGDGTFGSGAFYGAGGAFTSDLFAADFDG
                     DGDQDVAVTGSSINFPYTYFVSIWKNNGDGTFAGRVDYTTGSGPQTVFAADLDGDGDR
                     DLVTANLEDNKISVLKNNGDGTFAAKVDYATGFSPYYVFAADLDGDGDQDVAICLQYG
                     DAVSVLKNNGDGTFGIHAEYGTGTSSGAFSVYAADLDGDGDRDLVTANQTVDKVSILK
                     NNGDGTFALHVDYPTGKSPTVAVAADLDGDGKQDIAVTNQLDNTVSVLKNNGNGTFAT
                     KVDYGTNLLPWGLAAADLDGDGDQDLVGTVGSVLKNLSTVVSNTSVGRSVVVNLPNGV
                     TMSYDSIGAAGNTTVATSSTGPAPNTGFQILPLASPLYYDFTTTAAFVGPVTICFAYN
                     PAGLTPLQEAQLKVEHFVLGAWVDVTQSLDTASNRICARVTSLSPFALMAPTNPTGVT
                     REEIAGIPTKFTLAQNFPNPFNASTQIVFDLPGAGNRPVKLEVIDILGQRIATLIDQA
                     LEGGRYHITWDGKDGQGKPVASGVYFYRLQAGEFRETKRMTLLK"
     gene            complement(3385..4185)
                     /locus_tag="AC16FU_03985"
     CDS             complement(3385..4185)
                     /locus_tag="AC16FU_03985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR2993564.1"
                     /translation="MGSSLSRSCRYETATLISADITHFFVEPPAVEENRLSLTGPEAF
                     HCASVLRHKVGDRITCVDGQGYTYTVEIVQVKKGLVAGKILHRTVNRNEPKIKVTLAQ
                     GVTKVSKFDWVIEKGTEIGVSAFLPLLTEKSVAKRTDLEVWAQKAARLKRVALAAMKQ
                     SLRSVCPAILPPVKVDELARRLSDYDLSLLASFRPDAQPIFQIFSQRPSKPNRILLTI
                     GPESGLSPREEDSLLSSGVVPVSLGPRRLRTETAGLVLASQVLGWAGD"
     gene            complement(4268..>4725)
                     /locus_tag="AC16FU_03990"
     CDS             complement(4268..>4725)
                     /locus_tag="AC16FU_03990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR2993565.1"
                     /translation="AVYALTRPGMRVLDVGTGTCILAIAAVKLGAKTAVGCDTDPLAI
                     ANAWENCRLNKVEKRIRLAQGSLEQIRARKTYDLVLANLITNQVVEFLPDLVRRARPW
                     GYLVLSGVLARQQRRVLDSIAKQSLDLTAIGEQQEWLCCIAQKTSLRGE"
BASE COUNT          983 a         1293 c         1389 g         1060 t
ORIGIN      
        1 aaaattttct ttctatcgat cgcctccagt cggtaagact cgtacgagaa cgagcccata
       61 cgggtcgctt cgaccggtct acctattttt gtttcacccc tccctgaccc tcccctcaga
      121 ggggagggaa aagctcccca aaagggtgga atttcgattg acaaggaggg aaagcaccgt
      181 ttattacgac gtaatgtagt tttttttcta ctcattcaac cgtgtgattc gcttgaggag
      241 ggtgtgatgc ggcaaagaac ggtcttcttg acagtgattg ggttcttgtt attgccaacc
      301 ggcgtgtttg cctttcagcc gctttttgac acttggttag cctatgacgt gggcagtatt
      361 cagaaaggga ttttcgccgc cgacgtggat gggagcggcg gtttggattt gctggtggcc
      421 gattacggta ccaacaccgt ttcggttctt ctgaacaacg gcgacaaaac cttcgccccc
      481 aaggtcaact acgccaccgg cacccgtccg gatgcggtct ttgccgccga tttggatggg
      541 gatggcgatc aggacttggc ggtggtgaac tacggaagcc gcacggtttc cattctgaaa
      601 aacaacggca acggcacctt cgcggttaag gtggactacc ctaccggcaa cggccccaaa
      661 tccatttaca ttgccgattt ggacggggac ggggaccggg atttggcggt ggccaacgac
      721 agcagcaaca cggtatccat tctgaaaaac aacggaaacg ccacctttgc ggccaaagtt
      781 gactatgcgg ccggcaccaa tccggcctcg gtctttggtg ccaatctgga tggggacggc
      841 gacatcgact tggctgtcgc caatgccggc agcgacaacg tttccattct cttaaacaac
      901 ggggacggaa cttttgccgc caaggttgat tactccagcc ccggcaatcc attctctatt
      961 tatgcggcgg atttggacgg ggatggcgac cgggatttgg ccgtggcaag tgggtttgtc
     1021 agcggctccg gttttcgcat tttcaaaaac aacgggaacg gcacttttgc cgccggcgtc
     1081 ggatattcag taccgaccgg cggtggcggc ggtttgtcct ccgtggttct gaccgatgtg
     1141 gacggggacg gtgaccagga tttggtcgtt gccaatccgg cgacctccat ggtccatgtc
     1201 ggcaaaaacg acgggacggg tacttttttt tccggcccga cgatctgggc cgactatcct
     1261 tcgggaccag gcccgctcgc cctcttcgcg gcggatttgg acggggatac gtatccggat
     1321 ttggccgcgg ccaacggggg gaccaacagc atttccgtcc accgtaacga cgggaacgga
     1381 gcgttcgacc gccggcagga tatcaatgtg ggcagcggtg gtcattccgt ttacgccgcg
     1441 gacttggacg gggatttgga ccgggatttg gccgtggaaa gcggctacgg ctttgccatt
     1501 tacaagaatg gaggggatgg aaccttcggc tccggggctt tctacggtgc cgggggagcg
     1561 ttcacctcag acctttttgc cgcagatttt gacggggacg gcgaccagga tgtggcggta
     1621 actggtagct ctattaattt cccctatacc tacttcgttt caatttggaa gaacaacggg
     1681 gacgggacgt ttgccggcag ggttgactat accacgggga gtggcccgca gacggttttc
     1741 gcggccgact tggatgggga cggcgaccgg gacctggtta cggcaaacct cgaggacaac
     1801 aagatttccg tattgaagaa caacggcgac ggcaccttcg ccgccaaagt ggactacgcc
     1861 accggatttt ctccgtacta tgtttttgcc gccgatttgg acggggacgg agaccaggac
     1921 gtggctatct gtttgcagta cggcgacgcg gtttccgttt tgaaaaataa cggcgacggg
     1981 acttttggga tccatgcgga gtacggaacc ggaaccagca gcggcgcttt ttcggtctat
     2041 gccgccgatt tggacgggga cggcgaccgg gatctggtta cggcaaacca aacggttgac
     2101 aaggtttcca tcttgaagaa caacggggat gggacttttg ccctccacgt ggattacccc
     2161 accggaaaat cgccgaccgt tgcggtcgcg gccgatttgg acggggacgg gaagcaggat
     2221 attgcggtta ccaaccaatt ggacaatacg gtttccgttt tgaagaacaa cgggaacggg
     2281 acttttgcga ccaaggtgga ttatgggacg aatctactcc cttggggact cgccgccgcc
     2341 gatttggacg gggatggaga ccaggatttg gtgggaaccg tgggctccgt tttgaaaaac
     2401 ctctctacgg tggtgtccaa cacctccgtc gggcgctctg tcgtggttaa cttgccgaac
     2461 ggagttacaa tgtcgtacga cagcatcggt gccgccggaa acacaacggt ggcaaccagc
     2521 tccacgggcc cggcgcctaa taccggcttt cagattcttc ccctcgcttc ccccctctac
     2581 tacgacttta ccactaccgc cgctttcgtc ggtccggtta cgatctgttt tgcctacaat
     2641 cccgccggtc ttacacccct ccaggaagcc cagctcaagg tggagcattt cgttttgggt
     2701 gcctgggtgg acgttaccca gtcactggac accgccagca accgcatctg cgcacgggtg
     2761 accagtcttt cgccgtttgc gcttatggcg ccgaccaatc ctaccggcgt gacccgggag
     2821 gagattgcag gcattcccac gaaatttacc ctcgcgcaga attttcccaa tccgttcaat
     2881 gcgagcacgc agattgtgtt tgatttgccc ggagcgggaa accggccggt aaagttagag
     2941 gttattgaca ttctggggca gcggattgcg acgctcattg accaagcgct tgagggtggg
     3001 aggtaccaca tcacctggga tgggaaagac gggcagggga agccggtggc gagcggggtt
     3061 tatttttacc ggctgcaggc gggggagttc agagaaacca agagaatgac cctattgaag
     3121 taggggcaat tcttgaattg cccgtacagg aaattcttct aatcttccga tgggctgacc
     3181 cctccctcac cctcccctca aggggaggga aatctcttga ccggctcagg gcgagccggt
     3241 cttcctgctt ttgtttgacc tcaccctaac cttcgtttac aattccatag gtcaggcttc
     3301 cagcctgacc tgtcctccga ggttggcccg atacggctct accgagaaac ctcggctact
     3361 cgaattctgg gttgcgtaag ggaattaatc cccggcccag cccaggacct ggctggccaa
     3421 gacaagaccg gcggtttcgg tgcggaggcg gcgggggcct aaagataccg gaacgacacc
     3481 tgaggaaagg agagaatctt cttctcgcgg agaaaggccg ctctccgggc cgatggtgag
     3541 caaaattcga tttggtttcg acggtcgctg gctgaagatc tgaaaaatgg gctgagcatc
     3601 gggtcgaaaa gaggccagaa gggataaatc atagtcgctg agccgccggg ccaattcgtc
     3661 cactttcacc ggcggcagaa tagccgggca gacggagcgg agggattgct tcattgccgc
     3721 aagggcgacg cgcttgaggc gcgcggcctt ctgggcccag acctcgaggt cggtccgttt
     3781 ggcgaccgat ttttcagtca aaagcggtag gaaagcggag acgccgattt cggtcccctt
     3841 ttcaatcacc cagtcgaatt tgctaacttt cgtcactcct tgcgctaggg tgactttgat
     3901 tttcggctca tttcgattga cggttcggtg caggattttc cctgccacca gccctttttt
     3961 tacctgcacg atttcaaccg tgtaggtgta accctggcca tcgacgcagg tgatccggtc
     4021 gccgactttg tgccgcagga cagaggcaca gtggaaggcc tccgggccgg tgagggaaag
     4081 tcgattttcc tctaccgctg ggggttcgac gaagaagtgg gtgatgtcgg ccgagatcaa
     4141 agtcgctgtc tcatatctgc acgaccggct tagggacgag cccatacggg ttgcaccaac
     4201 cggtctacca gagaaatgtt gatttttgac ccctccctcg ccctcccctc taaggggagg
     4261 gaaaaatcta ctctccacgt agagaagttt tctgcgcaat gcaacagagc cactcctgtt
     4321 gttcacctat ggcggtgagg tcaagcgact gttttgcaat agaatccagc acccggcgtt
     4381 gctggcgcgc aagcactccc gagagaacca aatagcccca cggtcgggca cggcgaacca
     4441 aatcgggcaa gaattcaacc acctgattgg taatcaggtt tgccagaacc aggtcgtacg
     4501 tttttcttgc gcggatctgt tcgagcgatc cctgcgccaa tcgaatacgc ttctcaactt
     4561 tattcaggcg gcaattctcc cacgcgttcg caatcgccag cgggtcggta tcacaaccga
     4621 cggcagtttt agctcccagt ttcaccgccg caatcgccag aatgcatgtg ccggtgccga
     4681 catccagaac tcgcattccg ggacgcgtca acgcatacac cgcgg
//
LOCUS       DBNGZA010000191         1343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_671545, whole genome shotgun sequence.
ACCESSION   DBNGZA010000191 DBNGZA010000000
VERSION     DBNGZA010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_671545"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16FU_03995"
     CDS             <1..403
                     /locus_tag="AC16FU_03995"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR2993566.1"
                     /translation="MPKGGTIICTEGSEENARMARDFWKRGKVKVKLDFRVGDALSVI
                     DKVKGQFDIILCDIDKWQYPEAFKKAVPRLRKGGLFITDNLLWSGKVIEPNPDADTRG
                     IIEFTKLIYNAKNLLATLLPIRDGISVCVKL"
     gene            419..1078
                     /gene="nth"
                     /locus_tag="AC16FU_04000"
     CDS             419..1078
                     /gene="nth"
                     /locus_tag="AC16FU_04000"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR2993567.1"
                     /translation="MSERLEALKQRTKKIIAGLKKEYPDAKCALNFSNPLELMVATIL
                     SAQCTDKRVNLVTPALFAQYRTPEDYFRAPREELEAAIRTTGFYRNKAKSIKACCLGL
                     LERFGGRVPNRMKDLLSLNGIGRKTANVILGTAYGIAEGVVVDTHVMRLSIRLGLTRE
                     KNPEKIEKNLIKIVPKKDWIIFAHLLILHGRNICIARKPKCLECVVNKWCPSAVKFMK
                     R"
     gene            1104..>1343
                     /locus_tag="AC16FU_04005"
     CDS             1104..>1343
                     /locus_tag="AC16FU_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR2993568.1"
                     /translation="MTSPLSRRDFLQALTAGLALAGLSPVLAGEKTKTPKTAIPTRPL
                     GKTGYEATIFALGGQAKIEEWGGRDNAIEIIHRALD"
BASE COUNT          340 a          379 c          370 g          254 t
ORIGIN      
        1 gatgcccaaa ggcggcacta tcatctgcac tgagggcagt gaagagaacg cccgaatggc
       61 gcgggacttc tggaaacggg ggaaggtcaa ggtcaaattg gactttcgcg tcggcgacgc
      121 cctctcggtc atcgacaagg tgaaagggca gttcgacatc atcctctgcg acatcgacaa
      181 atggcagtac ccggaggcgt tcaaaaaagc ggtgccgcgg ctgcgcaaag gcgggctttt
      241 catcaccgac aaccttctct ggtcggggaa ggtgatcgag cccaatcccg acgccgatac
      301 gcgggggatc atcgaattca ccaaattgat ttacaacgcg aagaacctct tggccacgct
      361 tctgccgatc cgggacggca tttcggtctg cgtgaaactg taaggacagt ccaagaaaat
      421 gtctgagcgc ctggaggcac tgaaacagcg aactaaaaag atcatcgccg ggttgaagaa
      481 ggaatatccg gacgctaagt gcgccctgaa cttctccaat ccattagagc tgatggtggc
      541 caccattctc tccgcccagt gcaccgacaa acgggtgaat ctggtcacac ccgcgctctt
      601 tgcgcagtac cgcacgccgg aggactactt ccgcgccccg cgggaagaat tagaggcggc
      661 gatccgcacc accggcttct accggaataa agccaaaagt atcaaggcct gctgtctggg
      721 gcttctcgag cgcttcggcg gtcgagtacc gaaccggatg aaagatttgc tctctctgaa
      781 tggcatcggg cgcaagaccg ccaacgtcat cctgggcact gcctacggca ttgccgaagg
      841 agtggtggtc gatacccacg tgatgcggct ttccattcgc ctcggcctga cgagagagaa
      901 aaatccggag aaaattgaaa aaaatttgat aaaaatcgta ccgaagaagg attggataat
      961 tttcgcccac cttttgatcc tccacggaag gaacatctgc atcgcccgca agccgaagtg
     1021 ccttgaatgc gtggtgaaca aatggtgccc ttccgccgta aaatttatga agagatgaaa
     1081 acccgtcgac tctggagacc ctcatgactt cccctctttc ccgccgcgac tttctgcaag
     1141 cattaactgc cggactggcc ctggccggtt tgagtccggt tctggccgga gaaaaaacaa
     1201 aaactcccaa aaccgccatc cccacacgtc ccctgggaaa aaccggctac gaggcgacga
     1261 ttttcgcttt gggcgggcag gcaaaaatag aagagtgggg cgggcgcgac aacgccatcg
     1321 aaatcatcca ccgggcgctc gat
//
LOCUS       DBNGZA010000192         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_140482, whole genome shotgun sequence.
ACCESSION   DBNGZA010000192 DBNGZA010000000
VERSION     DBNGZA010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_140482"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="AC16FU_04010"
     CDS             complement(<1..564)
                     /locus_tag="AC16FU_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR2993569.1"
                     /translation="MLQIPAQRKVFFLLAFGFLLRFGLAWLPEKFLYYLISDDAYYYF
                     TIAGNFITRGMLSADGITLTNGFHPLWLFVITPVYFFFRSSPWLGIHLVMTIAAVFDT
                     AAAFLIYKTLKRLGKPNVGFWAALFYLANPYGLLHTMNGLETAQNNFFLAFLVFLSVR
                     STPDWLQKNWLFLGTICGLTLLSRTDNV"
     gene            1214..2110
                     /locus_tag="AC16FU_04015"
     CDS             1214..2110
                     /locus_tag="AC16FU_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993570.1"
                     /translation="MLLAQFFTGLFTDGAVCGEVVLSPAGDRIDRFYFIDPATIEFIP
                     NRNGQLELYQVTEDRPIRLNPHSTFYFGLDTDVANPRGRSLLSGIPFVTRVEQQLVAD
                     MQKTMHNAGYHRLHVKIKPPEKKDAESDDAYVARANRYFEDTVGMMKKIAPEDNPVTW
                     NEVEIAYIGPTGQYAGASGWYLNHKAMVEDIASGTHLDPFMLGYSYGTTQHWAGFKLE
                     LVERQVISVQRPAKRFVEWLHNLELALHGLDLRAEHHFDNRRTFGLLEARKAEQMHLD
                     NVLKQQRAGFLTFDEAKQRAIE"
BASE COUNT          604 a          637 c          671 g          520 t
ORIGIN      
        1 gacattgtcg gtgcgggaaa gcagggtcag gccgcaaatc gtccctagga aaagccaatt
       61 tttctgcagc cagtcaggtg tgctgcgtac tgaaagaaac acgagaaatg ctaaaaagaa
      121 gttattctgt gcggtctcca gcccgttcat cgtatgcagc aaaccgtagg ggttagccag
      181 gtagaaaaga gcggcccaga acccgacgtt tggtttcccc aatcttttca gagttttgta
      241 gatcaggaaa gcggcagcag tgtcaaaaac cgccgctatg gtcatcacca aatgaatgcc
      301 gagccacggg ctgctgcgga agaagaagta gaccggtgtg atgacgaaaa gccagagggg
      361 atggaagccg ttggtgaggg taataccgtc cgccgaaagc attccccgcg taataaaatt
      421 tccggctatg gtgaagtagt agtaggcgtc gtcagaaatg aggtagtaga gaaacttttc
      481 cggcagccag gccaagccga accgcaaaag aaagccaaag gctaagagga agaaaacctt
      541 cctttgcgct gggatctgca gcactaccgg aaagataaac ctcgctgcat tattttgata
      601 gcccggatcg cccctgagtt ggtcaggacg aactcctcga tggagaggaa tattgtcttt
      661 ctcctttcct aatctcttat cacttatcac tcatccttaa tcactgtttt atttggggtc
      721 atcatggggg taaaacggac aaagtgcgaa atagtaggag caggtcagtg gccaggtacc
      781 agtagccagt agccagaaag acaacaagat gacagttacg caacgtgtgc ggaaattctt
      841 gtttggaatc gacgcagaaa gctcatcgaa caaaaatgct gcgcaactca aggcaaatgg
      901 gttgaccgca caagccgagt tcattccgaa caagccggtg tcgcagagga atttcgagcc
      961 ggagccggta ttgtttttgc cggcgtagta tggttgggat aaccggacga ggttattctt
     1021 ctaccgtttc ctgcgggaca acattcccgc gctaaactcc gccatctgga cctggacgcg
     1081 cctctgcacc agcccgaatc gcgctgaagt caaaaccgac aacgcggcac tcaaaaccag
     1141 agcggaggaa attctggtcg acttagacca gcgcattttc gagcacgact tcgccaaatt
     1201 cagcgggttg gacatgctct tggcgcaatt cttcaccggg ctctttaccg acggcgcggt
     1261 ctgcggcgaa gtggtcctct cccctgccgg cgaccggatt gaccgcttct acttcatcga
     1321 tccagcgacg attgagttca ttccgaacag aaacggacag ctggaactgt atcaggtcac
     1381 cgaagaccgg ccgattcgtc tgaatcccca ctcgaccttt tacttcggtc tcgataccga
     1441 cgtcgccaac ccgcgcggga gaagtcttct ctccggaatt ccatttgtga cgcgggtgga
     1501 acagcagtta gtcgccgata tgcagaagac gatgcacaac gccggctacc accgcctgca
     1561 cgtgaagatc aaaccgccgg agaaaaagga cgccgaatcg gacgatgcct acgtggcgcg
     1621 ggcgaaccgc tactttgaag acaccgtggg gatgatgaaa aagatcgcgc cggaggataa
     1681 cccggtcacc tggaacgagg tggaaatcgc ctacatcggc ccgacgggac agtatgcggg
     1741 cgcgtccggc tggtatttga atcataaggc gatggtggaa gacatcgcct ccggcacgca
     1801 tctcgatccc tttatgttgg gttactccta tggcaccact cagcactggg ccgggtttaa
     1861 gttggagttg gtcgagcggc aggtgatttc ggtgcagcgt ccggccaaac gcttcgtgga
     1921 gtggctgcac aatttggagt tggccctgca tggacttgac ctgcgcgcgg agcaccactt
     1981 cgacaaccgc cggacttttg gcctgctcga agcgcgcaag gccgagcaga tgcaccttga
     2041 taatgttttg aaacagcagc gcgccggatt tttgacgttt gatgaagcaa agcaaagagc
     2101 cattgagtaa ttagcaacgc tgaccccacc ctgccctccc ctacagggga gggaaaatcc
     2161 atacttcacc ttcaatagaa agaatgaagg gtgaacttca ctccctctcc ttgtagtggt
     2221 tcggcttcgc tcaccaccct gagcctgtcg aaggggagag ggtcgggtga ggtcattacc
     2281 cctccccttt gaggggaggg tagggagggg ttgcctttgc ctccatagcc gaggcgtccc
     2341 cgcctcggtt ttggctcttt ctcaattctc atgcacatct caccaacctt ccgtgaaaaa
     2401 tggaaaaaat gctggcttga tccagtctat tt
//
LOCUS       DBNGZA010000193         7401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2793346, whole genome shotgun sequence.
ACCESSION   DBNGZA010000193 DBNGZA010000000
VERSION     DBNGZA010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2793346"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1502)
                     /locus_tag="AC16FU_04020"
     CDS             complement(114..1502)
                     /locus_tag="AC16FU_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2993571.1"
                     /translation="MALVLVIDDEILLGRSLSRALSQRGHDASAVGTAEEGLSLLEKL
                     APDIVLLDMQLPGLSGLDALKKIREADPNILVIIVTAYGTIASAVEAMRVGAVDFLRK
                     PLDTDEVAIAIERALAHARLARTVSFYQSKEAQRLAEEEFIGQSPKAREVQAWLKKVT
                     ELKVAKASDLPPVLVLGETGTGKDLVARLLHYRSPFAGLPFIEVNCPTLPQGLEEAEL
                     FGYEKGAFTDARQCKRGLAEVAEGGTLFLNEIGDLTPETQAKLLRLIEHKKMRHVGGL
                     RDVSIDLRIIAATNRNLAQAVKTGHFRQDLFYRLDHVTITLPPLRERGEDIILLADHF
                     LKKVSGSSGGAKKLSDAAHRVLLVYHWPGNVRELRHLMERALLLSSGTFIEPSDLRLQ
                     TADGAAVTIAPGQKVKVEFGPHGIDIEEVEKELISRALDEAGGNVSEAARMLKLGREA
                     MRYRIQKFGLES"
     gene            complement(1504..2952)
                     /locus_tag="AC16FU_04025"
     CDS             complement(1504..2952)
                     /locus_tag="AC16FU_04025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2993572.1"
                     /translation="MTIRSKLLFSYLSLLLAVVAIGVAAFWAAGRWRQSAAELSRTYN
                     QGLAAERLRADIYRQIAAGTDFLRGDRQAEQEFWDIQERVTAHLQQLKASAKAPEEID
                     HLGALEETQKELAYFAGEIFDAPMQTARNPKSDPGEKLDDIGEEVTSDVAALNQYYQV
                     REKESLAAASRSGNLTAYLVGGAALLAFVELLAMVFLLQRWLAGPIAEVGRVTARISR
                     GDFGARVARESTDEWGELSGAVNRMAESLKSYEHRLRAQERLAALGEVAAFAAHNIRN
                     PLAGIRAASQVMLAECVEERTAVEAREALTGIMQSVDRLDVWIGRLLNFAKPLALQPE
                     KADLRLVVAETLRLLNPALKEKKVKITTRVPEDLPPVFADPALLEQVLSSIITNAAEA
                     TAGDGSVEIQAVAASGEVSVKVSDTGKGMTPAFLERVFKPFVTDKPGGTGLGLAQAKK
                     IVDLHGGDITIQSRPGAGTTVTVHLPVWREGT"
     gene            complement(2949..3896)
                     /locus_tag="AC16FU_04030"
     CDS             complement(2949..3896)
                     /locus_tag="AC16FU_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2993573.1"
                     /translation="MKAFVTGGAGFIGSHLVSFLLKKGYAVTILDDFSSGRRENLCGL
                     EAATLVEGDVRDFEVVCQTAKGADYVFHLAAVVSVLGSLEDPQTTWEVNLKGSYNVLE
                     AARRAGVRRVIFISSSAVYGEYPKLPFKETFPAQPESPYALSKLMGEELCSLYWRLYQ
                     MEAVALRLFNVYGPRQNPFSQYANAIPNFTRTLLTGETPTIYGDGEQTRDFVAVSDVV
                     RAALLAGRVKKTVGQVVNVGSGKRLSVNRLFSEIQKILQTDARPQFFPRKQGDVLHTW
                     ADLRRARTVLGFVPRADFARALRETVLWFKNSGIFSRAR"
     gene            complement(4100..5635)
                     /locus_tag="AC16FU_04035"
     CDS             complement(4100..5635)
                     /locus_tag="AC16FU_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR2993574.1"
                     /translation="MLAKVFSSAVHGIDAYLVEVEADITATLPAFATVGLPDGAVKEA
                     KERVMSAIKNSDFIFPNKKVTINLAPADVKKEGSAFDLPMAVGILSATGQISRDDLTP
                     YVFLGELSLDGGLKPVDGVLPMALSLKTDGKKGLVVPRENAKEAAMAAGVPVFPVESL
                     SQAVKFLEGNVDIQPFTLDIDEVFNQALVYPLDFSDVRGQEHAKRALEVAAAGGHNIL
                     LIGPPGSGKTMLAKRLPTILPNLTLDEALETTKIHSVAGQLPPDTALIATRPFRSPHH
                     TISDAGLIGGGRMPKPGQVSLAHNGVLFLDEMPEFRKDVLEVLRQPMEDGYVTISRAL
                     VTLAFPAKFMLAGAMNPCNCGFFGDPGHECSCTPTQIQKYMSRISGPLLDRIDIHIEV
                     PAVKFRELAGQPTGESSAAIRERVNRARQVQLERFKSEKSIYCNAHMQSREIRKYCLI
                     DDKSQELLRLAMQKLGLSARAFDRILKVSRTIADLEGNAEIQPQHVSEAVQYRSLDRN
                     LWL"
     gene            complement(5742..6191)
                     /locus_tag="AC16FU_04040"
     CDS             complement(5742..6191)
                     /locus_tag="AC16FU_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993575.1"
                     /translation="MRRQTERVANAIFGLLFLCFLSGCSRKPQPKETLYRFFAGVVNR
                     DSTAIQDCLDLERTATEAQLQAIGVDSIPIVPEARQKFLIHELAAGRVNQRWREHRIV
                     VGETALRGDTAMVEVSFFHRPTGRQYYNKMGLIFSGGRWKIVSFKLL"
     gene            complement(6245..7189)
                     /locus_tag="AC16FU_04045"
     CDS             complement(6245..7189)
                     /locus_tag="AC16FU_04045"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993576.1"
                     /translation="MKKVFINSLVAGAALLCMSGDTQATNSKVGTTAYSFLKIGVGAR
                     AVALGGAFTGLADDESALYYNPAGLVSTGKRAVTTSYVNYLSDIQSGFVGYIHPYTET
                     QRFGVAVNYFNYGSFTGLDQTGAPTGDFTPSDLALAVSYAAGFKGGLQVGLSSRFVFE
                     KIDSLSSTGLMFDLGALYKFKDGRTQAGVALQNAGFQLSGLTSMHKDDLPVVVRVGFA
                     HFLRGLPLVFAADVAKPVDNDFHIDVGAEFLQLKPVYLRLGWSSIGSAIRTSSDNNKL
                     AGFAGGFGVIYQKYEIDYAFVPYADLGSAHRVTVAARF"
BASE COUNT         1539 a         2195 c         2114 g         1553 t
ORIGIN      
        1 cgaaaaagta tttctgaata agttattgat gagtaatagg ttaaatcata caaataaatc
       61 gagattacag agaaaactgg tttaattaaa caaaactgtt ttatttcaac aaatcacgac
      121 tccaagccga atttctgaat ccggtaccgc atcgcctccc ggcctaactt caacatgcgt
      181 gccgcttcgg aaacatttcc cccggcttca tccagggccc ggctaatcaa ctccttctcc
      241 acttcttcaa tgtcaatccc gtgcggcccg aactcgactt tgactttctg tcccggagcg
      301 atggtcaccg ccgcaccgtc agcggtttgc aggcggaggt cggacggttc gatgaacgtt
      361 ccagaggaaa gtagcaacgc ccgctccatc aggtggcgca gttcccggac gttgccgggc
      421 cagtggtaga ccagcaagac acggtgggcg gcgtcgctca atttttttgc gccgccggag
      481 ctcccggaga cttttttcaa gaaatggtcg gccaacaaaa tgatgtcctc tccccgctca
      541 cgcagaggcg gcagcgtgat ggtcacatgg tcgagccggt agaagagatc ctggcggaag
      601 tggccggttt tgactgcttg ggccagattc cggttcgtgg cggcgataat gcggagatcg
      661 atggagacat cccgcaggcc gcctacatgc cgcatttttt tgtgttcgat caagcgcagc
      721 aatttggctt gcgtttcggg cgtcaaatca ccgatttcat tcaaaaataa cgttccgcct
      781 tcggccactt cggccaggcc acgtttgcac tggcgggcgt cggtgaaggc ccccttttcg
      841 tagccgaaca gctccgcttc ctcaagtccc tgcggcaacg tgggacagtt gacctcgatg
      901 aacggcaggc cggcaaaggg gctccggtag tgcagaagcc gggcgaccag atctttgccg
      961 gtgccggttt cccccagaac cagaaccggc ggcaggtcgg aggctttggc cactttcaac
     1021 tcggtcacct ttttgagcca ggcttgcacc tcacgggcct tggggctctg gccgatgaac
     1081 tcttcctccg ctaagcgctg agcttccttg ctttggtaga aggaaaccgt ccgggccagc
     1141 cgggcgtggg ccagggcccg ctcgatagcg atggccacct cgtcggtgtc caggggcttg
     1201 cgcaggaaat cgaccgctcc caccctcatc gcctccaccg ccgaggcgat ggtgccgtag
     1261 gcggtgacga taatgaccag aatgttcgga tcagcctcgc ggattttttt cagtgcatcc
     1321 agacccgaaa gaccggggag ctgcatatcg agcaggacga tgtcgggggc gagcttctct
     1381 aagagcgaca ggccttcctc ggcggtgccg accgcggagg cgtcgtgtcc ccgctgggaa
     1441 agagcccggg agagcgaacg ccccaggagg atttcgtcgt cgatgacgag caccagggcc
     1501 atgttacgtt ccctcgcgcc aaaccggtag gtggaccgtc accgtcgtcc cggcgccggg
     1561 ccggctttgg atggtaatgt cgccgccgtg caggtcaacg atcttcttgg cctgcgccag
     1621 ccccagcccg gtgccgcccg gtttatcggt gacaaaaggt ttgaagaccc gctccaggaa
     1681 ggcgggtgtc atccctttac cggtgtcgga aactttcaca gagacttctc cagacgcagc
     1741 caccgcctgg atctccacgc tgccgtcgcc cgccgtcgct tcggcggcat tggtaataat
     1801 ggaagataaa acctgctcaa gcaaggccgg atcggcaaag accggtggaa gatcctctgg
     1861 cacccgggtc gtgatcttga cctttttttc tttcagcgcg gggttaagca ggcgtaaagt
     1921 ttccgctaca accaggcgga ggtcggcttt ctccggctgg agtgccaagg gtttggcgaa
     1981 attcaaaagc cggccgatcc agacgtccaa cctgtccacc gactgcatga tgcccgtgag
     2041 cgcctcccgg gcctcgactg cggtgcgttc ctctacgcac tctgccagca tcacctggga
     2101 agcggcgcgg atgccggcca atgggttgcg gatgttgtgg gcggcaaaag cggcgacttc
     2161 ccccagggcg gccagccgct cctgcgcccg gaggcggtgc tcatagcttt tgagcgactc
     2221 ggccatccgg ttgacggcgc cggataattc cccccactcg tcggtgcttt ccctggcgac
     2281 gcgcgctcca aaatcccccc ggctgatgcg ggccgtgacc cgtccgacct cggcgatggg
     2341 gccggccagc cagcgctgca acaggaagac catggccaga agctcgacaa acgccaaaag
     2401 cgcggctccc ccgacgaggt acgcggtcag atttccggaa cgggaagcgg cagccagtga
     2461 ttctttttcc cgaacttgat agtactggtt caacgccgcc acatcggagg tgacctcttc
     2521 gccgatgtcg tctaactttt cgccggggtc acttttcggg ttgcgcgccg tttgcatcgg
     2581 cgcgtcgaaa atttcaccgg cgaagtacgc cagttctttc tgcgtctctt ccagggctcc
     2641 gaggtggtcg atttcttccg gggctttggc ggacgccttc aattgttgca ggtgcgcggt
     2701 gacccgctct tgaatgtccc agaactcctg ttccgcctgg cggtcgcctc tgaggaaatc
     2761 ggtaccagcg gcaatttgac ggtagatgtc ggcccggagc cgctccgccg ccaatccctg
     2821 attgtaggtg cgggaaagtt cggccgccga ctgccgccag cgcccggcgg cccaaaaagc
     2881 ggccacgccg atggcaacca ccgccaaaag cagggaaaga taactgaaaa gtaacttgga
     2941 gcggatggtc atcgggctcg cgaaaaaatg ccggagtttt tgaaccagag cacggtttcc
     3001 cgcagcgccc tggcaaagtc ggcccgggga acaaaaccta agacggttcg ggcgcggcgc
     3061 agatcggccc aggtgtgcag cacatccccc tgttttcggg ggaagaattg cggcctggcg
     3121 tcggtttgca aaatcttttg gatttcggaa aaaaggcggt tgacggaaag acgtttgccg
     3181 gagccgacgt taaccacctg tccaaccgtt ttcttgaccc gaccggccaa aagcgccgcc
     3241 cgcaccacat cggagacggc aacaaaatca cgggtctgtt ccccgtcgcc gtagatcgtc
     3301 ggagtctcgc cggtcaaaag ggtgcgggtg aagttcggaa ttgcgttggc gtattgggaa
     3361 aagggattct ggcgcggacc atacacgttg aacagccgca gggccaccgc ttccatttgg
     3421 tacagccgcc agtacagcga gcagagttct tctcccataa gtttggacag ggcgtagggt
     3481 gattccggct gtgccggaaa ggtttctttg aagggcaatt tggggtactc gccgtacacg
     3541 gccgatgaag atatgaaaat cacgcgccgg accccggcgc gtcgggccgc ttccaggaca
     3601 ttgtagctgc ccttgagatt cacctcccag gtcgtctggg gatcttcgag agagcccaga
     3661 actgatacca ccgccgccag atgaaaaaca tagtcagctc ctttggctgt ttggcagacg
     3721 acctcaaagt cgcgcacgtc cccttctacc agtgtagccg cttcaagtcc gcagaggttc
     3781 tcccggcggc cgctggaaaa gtcatctaga attgtaacgg cgtacccctt tttcagcaaa
     3841 aacgatacaa ggtgggaacc gataaaaccc gccccgccag tgacgaacgc tttcacgtca
     3901 gtcctttttg gtgtctcttt ggacgccaca taatcattca aggtaatggg tgcgtttctt
     3961 attcacaagg ttttgctcat tccttgtgga tttgcgagta gagttgaaca aaacgaggcg
     4021 gactctttac tctgggaaca ggtaggtttt cataactaaa cacttatggc aaccggatcc
     4081 tttttcaccg cctatgttct cacagccata gattccggtc cagcgagcgg tactgcaccg
     4141 cctccgacac gtgctggggt tgaatctcgg cattgccctc caagtcggcg atcgtccgcg
     4201 acactttcag gatgcggtca aacgcccggg cggagagacc caacttttgc atcgccagcc
     4261 gcaggagttc ctgcgatttg tcgtcaatga ggcagtactt ccggatttct ctagactgca
     4321 tgtgtgcgtt gcagtaaatc gacttttcgc tcttgaagcg ctccagttgt acctgccgcg
     4381 cccggttcac tcgctcacgg atagcagccg aactctcacc cgtcggctga cccgctaact
     4441 cgcgaaattt tactgccggc acttcaatgt gaatgtcaat ccgatccaaa agcggcccgg
     4501 aaattctcga catgtacttc tggatttgcg tcggcgtgca ggaacactcg tgtccgggat
     4561 cgccaaaaaa gccacagtta caggggttca ttgcgcccgc taacatgaat ttggccggaa
     4621 acgcgagcgt caccaaagcg cgggaaatag tgacgtagcc atcctccatc ggctggcgca
     4681 aaacttcgag cacatccttg cggaactccg gcatttcatc taagaacagc acgccattgt
     4741 gggccagcga aacctgcccc ggcttgggca tgcgcccgcc gccgattaag ccggcgtcgg
     4801 aaattgtgtg gtgcggggag cggaaaggcc gggtggcgat caaagccgta tcgggtggca
     4861 gctgcccggc taccgagtgg attttcgtgg tctctaacgc ttcgtctaag gtgaggttcg
     4921 gaagaatggt cggcagccgt ttggcgagca tcgtcttgcc cgagccgggc gggccgatga
     4981 gcaaaatgtt atgcccgccg gcggcggcca cttccagcgc ccgcttggca tgctcctgtc
     5041 cgcgcacgtc ggaaaaatca aggggataga ccagcgcctg attgaagact tcatcgatgt
     5101 cgagcgtgaa gggttgaatg tctacattcc cttcgagaaa ttttaccgcc tggctcaaag
     5161 actccaccgg gaaaaccggc acgcccgcag ccatcgccgc ctctttggca ttctcccggg
     5221 gaacgaccag ccctttcttc ccgtcggttt tcagcgaaag cgccatcggc agaacgccgt
     5281 ccacgggctt gaggccgccg tccagcgaga gttcgccgag gaagacataa ggcgtcaggt
     5341 cgtcgcgcga aatttgtcca gtggcggaga gaatgcctac ggccatgggc agatcgaacg
     5401 ccgacccctc tttcttcaca tccgccgggg ctaagttgat ggtgactttc ttattgggga
     5461 agatgaaatc ggagtttttg atcgccgaca tcacccgctc cttggcttcc ttgaccgccc
     5521 cgtcgggcag gccgaccgtg gcaaaggccg gcagcgtggc ggtgatgtcc gcttccactt
     5581 cgactaagta cgcatcgatc ccgtggactg ccgaagagaa gactttggcg agcatggcag
     5641 agaaaaaatg accccccgga acccgtttgt caaaccgcct tcggggtcgc tcaagctctc
     5701 agcatatctt tttccgagga aagccggggt gtggtacgat gttagaggag cttaaatgac
     5761 actatcttcc agcgcccgcc tgaaaaaatt aaccccatct tattgtagta ctgcctcccc
     5821 gtcggccgat ggaagaagga aacttcgacc atcgccgtat ctccgcgcaa tgctgtttct
     5881 ccgacgacaa tccggtgctc ccgccagcgc tggttcaccc gtccggcggc caattcgtgg
     5941 atgaggaact tctggcgtgc ttccggtact atcgggatcg agtccacgcc aatggcctgc
     6001 agttgcgctt ccgtcgccgt ccgttccaaa tcgaggcagt cttggatggc ggtactgtcc
     6061 cgattgacca caccggcaaa aaacctgtac agcgtctcct tcggttgcgg cttccgcgaa
     6121 caaccggaga gaaaacaaag aaagagtaat ccgaagattg cattagcgac tcgttcggtc
     6181 tggcgcctca tcggcgaatt gctcgggaag gagaacgaac cgggatcagc tcgctcggct
     6241 gacgttagaa ccgcgccgcc accgtcaccc ggtgggccga cccgagatcg gcgtacggca
     6301 cgaaggcgta atcaatctcg tatttctgat aaatcacgcc gaaacctccg gcaaaccccg
     6361 ctaatttgtt gttgtccgaa ctggtgcgga tggcgctgcc gatactgctc caccccagac
     6421 gcaaatagac cggtttgagc tgcaggaatt cggcaccgac gtcaatatgg aaatcattat
     6481 ccaccggctt ggccacatcg gcggcaaaaa cgagcggcag tccgcgcaaa aagtgagcga
     6541 acccgacccg gaccaccacc ggcaggtcgt ccttatgcat ggaagtcagc cccgacaact
     6601 ggaaccccgc gttctgcagc gccacgcccg cctgcgtgcg accgtctttg aatttgtaga
     6661 gcgctcccag gtcgaacatc agtccggtgg aactgagact gtcgattttt tcgaaaacga
     6721 aacggctgga gagacccacc tgcaacccgc ctttgaagcc ggcggcgtag gagaccgcca
     6781 gcgcgagatc gctgggggtg aaatcgccgg tcggtgcgcc ggtttgatcc agcccggtga
     6841 aggaaccgta gttgaagtag ttgaccgcca ccccgaagcg ctgcgtttcg gtataggggt
     6901 gaatgtagcc gacgaagccc gattggatgt cggaaaggta attgacgtaa ctggtcgtca
     6961 ccgcccgttt accggtggaa accagtccgg cgggattgta gtagagtgcc gattcgtcat
     7021 ccgccagtcc ggtgaaggcg ccgcccaaag ccaccgcccg cgcccccacg ccgattttca
     7081 aaaacgagta agcggtcgtt cccaccttcg agttggttgc ttgcgtgtcg cctgacatac
     7141 agagcaaggc cgcaccagcc acgagactgt ttatgaatac ttttttcatg gaatgctcca
     7201 cggctgattt gttcaacatc tttctcataa atgtaacagt tttttttcaa tttgcaatga
     7261 aaatctcacc atgtccgcac cgtccggttg actatatcct gccccaattt cgtgattgcc
     7321 cgcgtgcggc cgtccgcttc ggattccgag gtagcgctgt agattccgaa ccctaaaaga
     7381 ttcttctcct cccagaccgt c
//
LOCUS       DBNGZA010000194         6261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_460484, whole genome shotgun sequence.
ACCESSION   DBNGZA010000194 DBNGZA010000000
VERSION     DBNGZA010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_460484"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16FU_04050"
     CDS             complement(<1..225)
                     /locus_tag="AC16FU_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR2993577.1"
                     /translation="MSLRGRAISFSGIGLTAMALACAKRGLPEGGPVDTTPPKIVFTE
                     PADRAVRVDSLATIRITFSERMESRSVQSNL"
     gene            complement(222..1724)
                     /locus_tag="AC16FU_04055"
     CDS             complement(222..1724)
                     /locus_tag="AC16FU_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993578.1"
                     /translation="MQKVRVRYDPRAYNHFTNGEIAFRLGDYATAISEFQQALAYDPA
                     SDEIRESLARVLVHANQYAQAATTAESIANKTADVWLLLGESYRNLNQRSQAIEAYRK
                     VTASDPENAQAWWYLSLLYHQEGDLAKAEEAAGKVWRLRPNHYYLEQYLSILLEEKKI
                     GAAITLLQKVIADDSTDYDAKLRLAEVYENADSTQAALELYRQVLGRMANRKQGEYKI
                     GQLYFRTDQFAPAESVFASLLAGDSSNFGAAVYYAWCALAQNHYADAEGRFKRVVALS
                     DSAYQGYSGLARVYLAQDSAAKAVPLLRQGLGKAPEHPELNYFLGRAFAALKQYDSAV
                     AAFRAAQAKEPNSQRVLFSLGSAFERGGQFDSAVAVFEEFLKLYPDDPGANNYLGYML
                     AEKGIRLPDAKAMIAKALAKEPENGAYLDSYGWVLFRLGQLSDAESWLRKAVEKLDTL
                     AVMRSDNAVIFDHLGDVLSLLGKTDEAKKQWERALALDPNNEKIREKLAR"
     gene            complement(1952..2992)
                     /locus_tag="AC16FU_04060"
     CDS             complement(1952..2992)
                     /locus_tag="AC16FU_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019145.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993579.1"
                     /translation="MRRVVRFFVTALFLLVGVGVSRGQVTRPDSDPPQADKPVVPGKV
                     KIDAERQALLARFKETYNVKTLRLNENGVPEYIKGELSKGLKAGEPVEEAYEFFQVNR
                     EVFHIRNPRSELALVKVERGPTGWTHLFFKQVYDSITVYGGDLRVHFVTNRELQIING
                     SYYPGIEVSTVPEITAEEAQVRVIHDLPCKVQPPKIHSTTLLIYPVTNSVKSGATVYH
                     LAWAVSTSVVDSCGSWGYFIDAHSGEILHRGSNMRELGGPADAGKREDSSTTKTKEVK
                     AKRLNIIEISPGDGRDSHSTSPLWGNDVLINGDLADQKSESLAAAKNGDLYVVFEDYD
                     PTTKPDASLPSP"
     gene            complement(3203..4030)
                     /locus_tag="AC16FU_04065"
     CDS             complement(3203..4030)
                     /locus_tag="AC16FU_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993580.1"
                     /translation="MKRTLWLAVAALVILPVVALAADVGNRDVVKFATARIEPGYVIV
                     PVEIVNDEQLAAVDLPLKWSEGATLEKVVFTERASAFQNFVNIKNADRTVIIAMIAFQ
                     SSPTPAVTAGLVGGSGPVAELYFKLNTGVSKLSVDATTMKKPSHQVMLIYNNKVDKSV
                     SVETISPEFQPFSLASLGAGNVPKTFALGQNAPNPFNPSTRINYALPVAGNVRIEVFN
                     ILGQKVKVLADGYEEAGYKSVVWDGKDDQGSQVASGVYLYRINALNFSDIKKMTLLK"
     gene            complement(4234..4536)
                     /locus_tag="AC16FU_04070"
     CDS             complement(4234..4536)
                     /locus_tag="AC16FU_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR2993581.1"
                     /translation="MVTKEVVLEALKDCYDPEIPVNIVDLGLIYDVQVQGDTVNVEMT
                     LTAPGCHMAAPISEEAKLRIEQLDGVKAANVKVVWEPPWTPERISPEAKKMLGFGE"
     gene            complement(4581..5402)
                     /gene="thiD"
                     /locus_tag="AC16FU_04075"
     CDS             complement(4581..5402)
                     /gene="thiD"
                     /locus_tag="AC16FU_04075"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR2993582.1"
                     /translation="MHVALTIAGSDSGGGAGIQADLKTFAAFGVFGTSAITAITTQNT
                     VAVTGVLELPAEMVAKQIDAVFTDFGVAAGKTGMLASARIIETVAERIMDYNLKKLVV
                     DPVMVSKSGHPLLEPEATATLIKKLLPLARVTTPNLPEAEVIIGKKLSSEADRKEAAR
                     KIFETTGAAVVLKGGHLSGSAVDIYYDGTGFKELFAERVETKNTHGTGCTFSAAIAAN
                     LALGLDLWEACRIAKEYITQTLKSSVTLGHGFGPVNHFPQATVPFHGVEMPARVG"
     gene            complement(5507..>6261)
                     /gene="nadA"
                     /locus_tag="AC16FU_04080"
     CDS             complement(5507..>6261)
                     /gene="nadA"
                     /locus_tag="AC16FU_04080"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR2993583.1"
                     /translation="DVLRAWDEVTAVIPKEKIIPLSYMNSAASLKAFCGKNGGLICTS
                     SNARKAFEWVFARAEKLFFFPDEHLGRNTANAMGIPPEKVIVWDFKQKLGGNSTEKIQ
                     NAQVILWKGYCHVHVTFGSDQIQEMRAKYPGIKVVVHPECTPDVVALADAVGSTTQIV
                     NYVELMPSGSTIAIGTEINLVSRLAHEHPDMQIMQLNGVRCSMCVNMFRTSLYDLLFT
                     LDNLGKINIIRVDPEIKRDALIALNRMLEVAS"
BASE COUNT         1339 a         1876 c         1604 g         1442 t
ORIGIN      
        1 gagattggac tgaaccgagc gggactccat ccgctcgctg aaggtgattc gtatcgtcgc
       61 cagcgaatca acccggacgg cccggtcagc cggctccgtg aagacaatct tgggcggcgt
      121 ggtatcgacc gggccgcctt ccggcaatcc ccgcttggcg caagcgagcg ccattgcggt
      181 caggccgatt ccggagaaag aaatcgcccg cccccgcaaa ctcaccgagc cagtttctcc
      241 ctgatctttt cattattggg atccagcgcc agcgcccgct cccactgctt tttggcttca
      301 tcggttttgc ctaaaagcga aagcacgtca cccaggtggt caaagatcac cgcattgtcg
      361 ctccgcatga ctgccagtgt gtctaacttc tccaccgcct tgcgcagcca ggattccgca
      421 tcggagagct gtcccaggcg gaagagcacc catccgtaac tatccaggta ggcgccattt
      481 tccggctctt tggccagggc cttggcgatc atcgccttgg catccggaag ccggatccct
      541 ttctccgcca gcatgtatcc taagtaattg ttcgccccgg gatcatcggg gtagagtttc
      601 aagaactcct cgaagaccgc caccgccgaa tcgaactgcc ccccccgttc aaaagccgat
      661 ccgagagaaa acaacacccg ctggctgttt ggttctttgg cttgagccgc gcggaaggcc
      721 gccaccgccg agtcgtactg tttgagcgcg gcaaacgccc ggcccagaaa gtaattgagt
      781 tccggatgct ccggcgcttt gcctaatccc tgacgcagga ggggcaccgc tttggccgcc
      841 gaatcttgtg ctaaatagac ccgcgccagc ccgctgtacc cctggtaggc cgaatcggac
      901 agcgccacca cgcgcttgaa ccgcccctcc gcgtcggcat agtggttctg cgccagcgcg
      961 caccaagcat agtagaccgc cgcgccgaaa ttgctggaat cgccggccag aagagaggca
     1021 aagacgctct cggccggggc gaactggtcg gtgcggaagt agagttgccc gattttgtac
     1081 tccccctgct tccggttcgc catccggccc aggacctgcc ggtagagctc cagcgccgcc
     1141 tgagtgctgt ctgcattctc gtagacctcg gccaggcgca gcttcgcatc gtaatcggtg
     1201 gaatcgtcgg cgatgacctt ctggaggaga gtaatggccg ccccaatttt tttttcttcc
     1261 agcaaaatcg agaggtactg ttccaagtag tagtgattcg gccgcagacg ccagaccttc
     1321 cccgccgctt cttccgcttt ggccagatca ccctcctggt gatagaggag cgacagatac
     1381 caccaggcct gggcattttc cggatcggac gccgtgacct tccggtaggc ctcgatggct
     1441 tggctccgct ggtttaagtt gcggtaggac tcccccaaaa ggagccagac atcggccgtc
     1501 ttgttggcaa tcgattccgc cgtggtcgcc gcctgcgcat actggttggc gtgcaccaaa
     1561 acccgcgcca gcgattcccg gatttcgtcg gaggcgggat cgtaggccag cgcctgctga
     1621 aattcagaaa tggcggtggc gtaatcgccc agccggaagg cgatctcgcc gttggtgaag
     1681 tgattgtacg cccgcgggtc gtagcggact ctgaccttct gcaaaggccc gcttttaacc
     1741 gtcgcctttg gcccggcaca gccgaaaaga aagaaaaatc ctgcaacaaa aattgaggaa
     1801 aacgcaagcc ctttgaccca acgcgacatc cgaagtcaat ttacgcctct ttgcgaacta
     1861 tttcaagtcg cagaccccgc cacaggcggg gtagaaagca tttcccacat tcactaaata
     1921 ctacggtcgt gaactttttt cctcccctta cttaagggga gggtagggag gcgtcaggtt
     1981 tcgtcgtcgg atcataatcc tcaaaaacga cataaaggtc cccatttttg gccgccgcca
     2041 gcgactcgct cttttgatcg gccaaatctc catttatgag aacgtcattg ccccagagcg
     2101 gtgaagttga atgtgaatcc cgaccatcgc ctggagatat ttcgattatg ttcaatcttt
     2161 tcgccttgac ttctttagtc ttcgtggtgc ttgagtcttc ccgtttacca gcatcagcag
     2221 ggccacctaa ctctctcata ttacttcccc tatgcagaat ctctccgctg tgggcatcta
     2281 tgaagtaccc ccaggatcca caagaatcga ccacggaagt gctcaccgcc cacgccaaat
     2341 ggtaaaccgt ggccccagat ttgacggaat ttgtcaccgg ataaataagc agtgtcgtag
     2401 aatggatttt tggcggctgg actttgcagg gcagatcatg aatcacccgc acctgtgctt
     2461 cttccgcagt aatctccggc accgtcgaaa cctcaatgcc cggatagtac gatccattaa
     2521 taatttgaag ttctctatta gtgacgaagt gaacacgcaa gtccccccca tacactgtga
     2581 tgctatcgta aacttgttta aaaaaaagat gggtccaccc tgttggaccc cgttcaactt
     2641 tgactaacgc aagttccgag cggggattac gtatatgaaa aacctcccga tttacttgaa
     2701 aaaattcata tgcctcttcg actggttcac ccgctttaag cccttttgat agttcgccct
     2761 ttatgtactc tggcaccccg ttctcattca gccgcaacgt cttaacgtta taagtctcct
     2821 taaaacgggc caggagggcc tggcgctcgg catctatttt tactttgccg ggaacaacgg
     2881 gcttatccgc ctgaggcgga tccgaatccg gccgggttac ttgtcctctg ctaaccccaa
     2941 cccccacaag gagaaacagt gcggttacga aaaatcgaac gactcttctc atcgccagtc
     3001 ttgttgtaca tagttaacgt agtctgtagc tccaacttct gtcaagtttt tctcggtaga
     3061 ccggtcggta cgacccgtat gggctcgtct ccgagccagt ctcggtgtcc atctgctaat
     3121 ctgcttcatc agctgtgaag aaccatcccc aaacaaaaac cccgccaaag gcggggcttt
     3181 tgagactttc ctggctgaag atttacttca gcaaggtcat cttcttgatg tcggagaagt
     3241 tcaaggcgtt gatccggtag aggtagacgc ccgaggcaac ttggctgccc tggtcgtcct
     3301 tgccgtccca gaccaccgac ttgtacccgg cctcttcgta gccgtcggcc agaaccttca
     3361 ccttttggcc cagaatgttg aacacctcaa tccgcacgtt cccggccacc ggcagagcgt
     3421 agttaatccg ggtggagggg ttgaacgggt tgggcgcatt ctgtcccaaa gcgaaggtct
     3481 tgggcacatt gcccgctccc agagaggcga gcgagaacgg ctggaactcc ggcgaaatcg
     3541 tctccacgga gaccgacttg tcgaccttgt tgttgtaaat caacatcacc tggtgcgacg
     3601 gtttcttcat ggtcgtcgcg tccaccgaca acttcgatac gccggtgttc aatttgaagt
     3661 agagctcggc caccggtcca gaacctccca cgagtccggc ggtcaccgcc ggcgtcgggc
     3721 tgctctggaa ggcgatcatc gcaatgatga ccgtgcggtc cgcgttcttg atgttgacga
     3781 agttctggaa cgcgctcgcc cgttcggtga agaccacctt ctccagcgtg gccccttccg
     3841 accacttcag gggcaggtcc accgccgcca gttgctcgtc gttcacgatt tccaccggca
     3901 caatgacgta gccaggttcg attcgcgcgg tcgcgaattt gactacgtcc ctgttgccga
     3961 cgtccgccgc cagtgctaca accggaagaa tcaccagcgc ggctacagcc aaccacagtg
     4021 tacgcttcat cagcatacct cctgtaaaag ttttactgca aattccctgc cggcgctttt
     4081 gggacggtgc cgcaaccata ccaaaatcgc ccgcttcgct tctctacttt cgcaaaacac
     4141 gcaccaggta ttgccgttct ctttcgggcg tgtggggtta tcctcttttc aaaggtcaat
     4201 atacaccccg gccaaaatgc tgtcaagtaa aatttactcc ccgaacccta acattttttt
     4261 ggcctccggg ctgatccgtt ccggcgtcca ggggggctcc cagaccactt taacattggc
     4321 cgcctttacc ccgtccaact gctcaatccg taatttcgcc tcctcagaaa tgggcgccgc
     4381 catatggcac cccggagcgg tcaacgtcat ctcgacatta accgtatccc cttgtacctg
     4441 aacgtcatag attagcccta agtcaacgat attgaccgga atttccggat cgtaacagtc
     4501 ttttaaggcc tccaaaacca cctctttggt taccaagaac tctcctcttt aaggttaaac
     4561 aaatttcggg tcaaaaagtt tcatcccacc cgcgccggca tctccactcc gtggaatggt
     4621 acggtcgcct ggggaaaatg attgaccggg ccgaagccgt ggcccagagt caccgaactt
     4681 ttcagggtct gggtaatata ctctttggcg atccggcagg cctcccacag atcaagtcct
     4741 aacgccagat tggcggcaat ggcggctgaa aaagtgcagc cggtcccgtg cgtatttttg
     4801 gtttcgaccc gttcggcaaa gagttcctta aacccggtcc catcataata tatatcgacc
     4861 gctgaacccg ataaatgacc tccttttaag acgaccgccg caccggtggt ctcaaagatt
     4921 ttcctcgccg cctcctttcg gtcggcttcg gaggaaagtt ttttcccgat gattacctcg
     4981 gcttcgggca ggttgggagt agtcacccgg gccaggggga ggagtttttt tatcaaagtc
     5041 gcggtggcct caggctccag caggggatgg ccgctcttgg aaaccatcac cggatcaacg
     5101 accaatttct taaggttgta atccattatt cgctcggcaa ctgtttcaat gatgcgcgcg
     5161 ctggcgagca tcccggtctt gccggcagct acaccgaagt cagtgaagac tgcatcgatc
     5221 tgcttggcca ccatctccgc cggaagttcg aggaccccgg tcacggccac cgtattctgg
     5281 gtggtgatag cggtaatcgc ggacgtgccg aacaccccga aggccgcaaa agtcttgagg
     5341 tcggcctgaa ttcccgcccc gccgcccgaa tccgacccgg ctatggtcaa cgcaacgtgc
     5401 atattgtctt caaatctacc agcaacgcgt aggcggcact ttactcgtgg tgagcgcagt
     5461 cgaaccagtg ccgcgcgggt ttccttgaaa taaaagaaag cttgaattac gacgctactt
     5521 ctaacatccg gttgagggcg attaacgcgt cgcgcttgat ttcgggatcg acccggatga
     5581 tgttgatctt tcccaggttg tccaacgtaa agagcaaatc atagagactt gtccggaaca
     5641 tattcacgca cattgagcag cgcacgccgt tcagctgcat gatttgcata tccgggtgct
     5701 catgcgccag ccggctgacc aggttaatct cggtgccgat ggcgatcgtg ctcccggagg
     5761 gcatcaactc cacgtaattg acaatctgcg tcgtggaacc gacggcatcg gctaaggcca
     5821 cgacatcggg cgtgcattcc gggtgtacca cgactttaat tcccgggtac tttgcccgca
     5881 tctcctgaat ctggtcagag ccgaacgtaa cgtgaacgtg gcagtacccc ttccagagaa
     5941 tcacttgcgc gttctgaatt ttttcagtcg aattgccgcc taatttctgc ttaaagtccc
     6001 agacgatcac cttctccggc gggatgccca tcgcgttggc cgtattgcgg cccaagtgtt
     6061 cgtccgggaa gaaaaagagt ttttctgctc gcgcaaaaac ccactcgaac gccttgcgcg
     6121 cgttggagga ggtgcaaatc aggccgccgt ttttgccgca gaaggcctta agcgacgcag
     6181 cggagttcat ataggaaagg ggaatgattt tttctttcgg aattactgcg gtgacctcat
     6241 cccacgcacg caggacgtct t
//
LOCUS       DBNGZA010000195         3439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_565228, whole genome shotgun sequence.
ACCESSION   DBNGZA010000195 DBNGZA010000000
VERSION     DBNGZA010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_565228"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2109
                     /locus_tag="AC16FU_04085"
     CDS             58..2109
                     /locus_tag="AC16FU_04085"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR2993584.1"
                     /translation="MAEASEPKPAAKKVGKDPASGGTEVKQTKTGGDHQPEKAKKVST
                     SPTAADTKPAERPAEARRGPERGRGAPPRRRGAKPPPRRRTERVGVSAPAEPASGGPA
                     PGVRAETKPKLPPVKPISEFEFEPTSPEEFEKLLPLYESTFKTVREGELVKGRVLRIT
                     RDDVFLDVGFKSEGIIPLVEFGENPEVKPGDEIDVFLESFEDQNGQLVLSKQKADFMR
                     VWDNIKEASDRNQPITGRAVRRIKGGLVVDLMGVEAFLPGSQIALRQQFDVDTLLGQA
                     LEFKIIKLNKARRNIVVSRRAVLEEQREVMKSKLLAEMAVGQTREGYIKNITDFGVFV
                     DLGGVDGLLHITDMAWGRVGHPSEMVNVGQKINVKILDFDKNTGRISLGLKQLTPYPW
                     ENVEQKFPVGSRVRGRVTSITDYGAFVELEKGLEGLVHISEMSWTHHVKHPSKILSAN
                     DVIEAVVLNVDREHEKISLGLKQLTADPWDTLELRYPVGIKVTGKVRNLTPFGAFVEV
                     EEGIDGLIHVSDMSWTRRIQHPSEVLKKNDVVEVKVLHIDKENRRISLGLKQVQEDPW
                     PKLAEKYRQGTEVEGKVMRLLERGVVVDIQNELEGFVPLNHLGVSDLAKPQDAFKEGE
                     TIPLRVLEFDQSGRRLMLSVDAYFQNRDKKELEEYRSKHPVKKAKEEPVGAAQEKEEQ
                     G"
     gene            2218..2721
                     /locus_tag="AC16FU_04090"
     CDS             2218..2721
                     /locus_tag="AC16FU_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2993585.1"
                     /translation="MAGNVKPIPEGYHSLTPHLVVNDGRRAVEFYQKAFGAEVRGVFQ
                     DSPDGKVHHAELKIGDSLFMLADEFPMEPKAGMKFLSPKSLGGSTTTLHLYVENVDAA
                     FDRAVAAGAAVAMPLMDAFWGDRYGQLLDPFGHRWALATRKENLSQEEVKKRGEAFFA
                     QWAKQKK"
     gene            2766..3344
                     /locus_tag="AC16FU_04095"
     CDS             2766..3344
                     /locus_tag="AC16FU_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993586.1"
                     /translation="MQSALAKEKIGDAALKAKTGKTWREWYAILDKAGARKMNHTEIA
                     AHLYEKEKVPGWWCQMVAVGYEQARGLRQKHERPDGFEISASKTIAASLATIYRAWKD
                     EKLRQRWLPKVTFSLTTDTPNKCLRMKWGEGSERVDVAFFPKGTGKGQITVQHRRLPT
                     ATAAEKMKTYWQKQLGGLKTLIENSASAGRKD"
BASE COUNT          839 a          950 c         1055 g          595 t
ORIGIN      
        1 cagatggata gaacgttgtt cgttggaggt tgtcgcgtaa cgtaaaggag gaacgtagtg
       61 gccgaagcaa gtgaacccaa gcccgccgca aagaaggtcg gcaaagatcc cgccagcggc
      121 gggactgagg tcaaacagac caagacagga ggtgatcacc aaccggagaa agcgaaaaaa
      181 gtttccacaa gccctaccgc agcagatacc aagccggctg aacggccagc cgaagcgcgc
      241 cggggcccgg agcggggccg cggtgctccc ccgcgccgca gaggggccaa gccgccgccg
      301 cgcagaagaa ctgagagagt cggcgtaagc gcgccggcag agcccgcctc aggcggaccg
      361 gcaccggggg ttcgcgccga gaccaaaccg aaactgccgc cggtcaagcc gatttccgag
      421 tttgaattcg aacccacctc gccggaagaa ttcgaaaaac ttttgcctct gtacgagtcg
      481 accttcaaga ccgtccgcga aggggaactg gtcaaaggcc gggtcttgcg cattacccgc
      541 gacgacgtct tcctcgacgt cggcttcaag tcggaaggga ttatcccctt agtcgaattc
      601 ggggagaatc ctgaggtcaa gccgggcgat gaaattgatg ttttcttaga atcgttcgaa
      661 gaccagaacg gccagttagt tctctccaag cagaaggccg acttcatgcg cgtctgggac
      721 aacatcaagg aggcctccga ccggaatcag cccatcacag gccgggcggt ccggcgcatc
      781 aaaggcggct tggtcgttga cctgatgggc gtggaggcgt ttctgcccgg ttcgcaaatc
      841 gctttgcggc agcagttcga cgtggatacc ctcctgggcc aggcgctgga attcaaaatc
      901 atcaaactga acaaagcccg gcgcaacatc gtggtctccc gccgcgcggt tttagaagaa
      961 cagcgcgagg tgatgaaatc gaaactcctg gcggaaatgg cggtgggcca gacgcgggag
     1021 gggtacatta aaaacatcac cgacttcggc gttttcgtcg atttaggcgg cgtcgacggc
     1081 ctcctgcaca tcaccgacat ggcctggggc cgggtgggtc accccagcga gatggtgaat
     1141 gtcggccaga aaatcaacgt caaaatttta gatttcgata aaaacaccgg gcgcatttcg
     1201 ctgggcttga agcagctcac tccctacccc tgggagaacg tggagcaaaa gttcccggtg
     1261 ggctcccgcg tgcgcggccg ggtgacctcc atcaccgact acggtgcctt cgtcgagctg
     1321 gagaaggggc tggagggcct ggtgcacatc tccgagatgt cctggaccca ccacgtcaag
     1381 cacccgtcga agattctctc cgcgaacgac gtgatcgaag cggtggtgct gaacgtggac
     1441 cgcgagcacg aaaaaatctc tctgggcttg aagcagctga ccgccgaccc ctgggacacc
     1501 ttggaactcc gctacccggt ggggatcaaa gtaaccggca aggtgcgcaa cctgacgcct
     1561 ttcggcgctt tcgtcgaagt cgaggaaggg atcgacggct tgatccacgt ctccgatatg
     1621 tcctggacgc ggcggattca gcacccctcc gaagtgctca agaaaaacga cgtggtggag
     1681 gtgaaggtgc tccacatcga caaggagaac cgccgcatct cgctcggttt gaaacaggtg
     1741 caggaagacc cctggccgaa attggccgaa aagtaccggc agggaaccga agtcgaaggc
     1801 aaagttatgc gccttttgga gcggggcgtg gtggtggaca tccagaacga actcgaaggt
     1861 tttgtgccgc tcaaccacct gggtgtttcc gacctcgcca agccgcagga tgcgttcaaa
     1921 gagggcgaga cgattcccct gcgcgttttg gaattcgacc agtcgggccg gcgcctgatg
     1981 ctctcggtcg atgcctactt ccagaaccgc gacaaaaagg aattggaaga gtaccgttct
     2041 aaacatccgg tgaaaaaggc caaggaggag ccggtcggag cagcgcagga gaaagaggag
     2101 caagggtaag gaagagcaat acattcacag cagataaagc agattaaacg gaaagagagt
     2161 aacaacaggc actccccggg gtgagctttc tttggttttt ttaaggagga tcctccaatg
     2221 gccggaaacg taaagcccat tcctgaaggt taccactcgc tgaccccgca cttagtggta
     2281 aatgacggcc gccgggccgt cgagttttac cagaaagcct tcggcgccga ggtgcgcggc
     2341 gtttttcagg acagtcccga cggcaaagtg caccatgccg aactcaaaat cggcgattcg
     2401 ctctttatgc tcgccgacga gttcccgatg gaaccaaaag cgggaatgaa attcctttcg
     2461 ccgaagtctt taggcgggag cactacgacc ctgcatctct acgtggaaaa cgtggatgcc
     2521 gcctttgacc gggccgttgc cgccggcgct gctgtggcca tgcccctgat ggacgccttt
     2581 tggggcgacc gctacgggca gttgcttgat cctttcggcc accgctgggc tctggcgacg
     2641 cgcaaggaga acctatcgca agaagaggtc aaaaagcgcg gcgaggcctt ctttgcgcag
     2701 tgggcgaagc aaaaaaagta accggactct cgaggggcac aatcatcggg ggaaagtgag
     2761 gaacgatgca gagcgcgctg gccaaagaaa aaattggtga cgcggcctta aaagcgaaaa
     2821 caggcaagac ctggcgcgag tggtatgcaa ttctcgacaa agccggcgcc agaaagatga
     2881 atcacaccga gattgccgcc cacctctacg agaaagagaa agtccccggc tggtggtgcc
     2941 agatggtggc ggtgggctac gagcaggcgc gcggcctgcg ccaaaagcac gagcgacccg
     3001 atggttttga aatcagcgcg agcaagacca tcgccgcgtc gcttgcgacg atctaccggg
     3061 cctggaagga cgaaaaactg cgccagcgct ggctgccgaa agtaaccttc tcgcttacga
     3121 ccgatacacc caacaagtgc ctgcggatga aatgggggga aggctccgag cgcgtggacg
     3181 tggcgttctt cccgaaaggc acggggaagg ggcaaatcac cgtccagcac cgcaggttgc
     3241 cgactgccac cgccgccgag aagatgaaaa cctactggca aaaacaactg ggaggactaa
     3301 agactctgat cgaaaattcg gcctcggcgg gtcgcaagga ttgatgttac tctgacgcca
     3361 tggtaagatt tattgcagta attgttgccg tcgttttggt cagcgccatc gcttttgcca
     3421 taagaaaatc caaaagcag
//
LOCUS       DBNGZA010000196         7830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1416452, whole genome shotgun sequence.
ACCESSION   DBNGZA010000196 DBNGZA010000000
VERSION     DBNGZA010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1416452"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="AC16FU_04100"
     CDS             complement(<1..965)
                     /locus_tag="AC16FU_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HIR2993587.1"
                     /translation="MNRLITDAPLIFFLGAGATKCLGKRLMRDFVDDFSIQVRNDQNF
                     PYMERELLEKMLKKEYDLEFLLQELEEISSKEYLPALSGYTQPAESLSLKLKGEIFKH
                     YSPIIAGDAAEYLKWVLEHSHFTFQLLNSTKDTEILPIFTTNYDPAIEEACKFGNFWQ
                     RNINFCNGIIQDFNSQEFVWDRRGFEFNPDPRKKQICLFKLHGSVDWYRINGKIVRVP
                     FSEHHGDDDIIRNVIIYPAMKKVAIAEPFLTAYNYLHHCLLNTNVAVFIGYSFRDYDV
                     LSRIKFALTENPNLMLIIFDPQAKNLRIKVFGLEYSGHVFIVEGN"
     gene            complement(981..4199)
                     /locus_tag="AC16FU_04105"
     CDS             complement(981..4199)
                     /locus_tag="AC16FU_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR2993588.1"
                     /translation="MMLKRALFLFLGFGIVRVSASQADSYWQQEVAYTIDCQLFPKGD
                     SLTGTETLVYTNHSPDTLTYVYFHLYLNAFQPGSYMDLRSREAGNFSLRDLPKRDWGE
                     ERIDSLAIEGERWTKSELDNTILKVDLPKPLLSGEKIAFFFKFVDKIPRNGSRMGKQG
                     RHYDIGQWFPRLAAYDKRGWHDDQYLGSEFYADYGTFDVSITVPKEYVLAHTGKLTNE
                     EELFPGGLPKAEGDSVVIDILKLYKEQHPSEKQIAFDSTNPDTSASPSIRQRPTRGGK
                     RAEPRHPEMQNPNGDSSPLDTAGKKNEPKTWTWKCHADTVFDFAFAADPDFIWDRAQW
                     EGIVMNALYQKDKQEFWQKWGAIYTREIIKFYSQTFGMWPYPQFSSVEGSVGGGIEYP
                     QMTIITRRSGSKYSHGLFAILAHEIGHNWFYGLLGNNETRQAFLDEGFTSFATCLAME
                     HYYGRYDNNYTWEKWYQKKFYPNGDERKDTFFSYLRFAKTGTEDVIDTHSDRFLPGSY
                     TNAVYPKTASILFMLQYVLGDSVFAKAMHDYYDRWHYKHPHKEDFYASIEKSSGCHLE
                     WFFQEWFERVYTCDYSLEGVKSELKDPNRPAPYDAEVKIKRRGRAIMPLDVQLTLADG
                     QKVSRDIPFDLWMKGPTLQTITIPVSSPVKRAVLNPDLRVQDVNRLNDYHPRAPVTFQ
                     FQPPQFLFPGYSPDPPLDAYQIRYQPKLWYNQEDVARLGFGGFGSYLGVDHRLNTDLW
                     VGVRHAQVDFNVNYTSPFYLFDNRTTWSAGGSFLEGRQGASLSLSRTYGRSGFFGFGG
                     FTAAPPRRVSLSWNYSRLDHPDYLSRTQSWSSGRVSTWKFSWNAVFNRWKFDSRIALE
                     LTSAALRTQHDFGKAALEIESDWHTPDKFPLWSRLYFGYAEGDVPLQDLFYVASASPD
                     EQFGSRWYSSRGSLPEGLKRTGNLYLPGGGNLRGFLKKNAGYKRIASANVRLGFENPL
                     TKLLTKIHQGSEILWRDLTSIETHAFFDFGQGWNSGNIPDLGDFFSDLGLSLSYRFPY
                     QSIRRLVGDNYIRVYFPLWISDPLPGESRWKFRWVLAYAAKF"
     gene            complement(4310..5041)
                     /locus_tag="AC16FU_04110"
     CDS             complement(4310..5041)
                     /locus_tag="AC16FU_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR2993589.1"
                     /translation="MSIIALKNVSKRFNQVVALDGVSLSVEPGEVIVLVGPSGSGKST
                     LLRCMNALEIPDSGEVIVDGIMLDHKKAHIYAIRRETGMVFQQFNLFPHLSVLENIYL
                     AQRVVRKRTKAEAGKIAETLLEKVGLLEKKAAYPGQLSGGQQQRVAIARALATNPKIM
                     LFDEPTSALDPEMIGEVLGVMKKLAREGMTMVVVTHEMGFAREVAHRIAFLDEGKLVE
                     IGPVEKVLRAPENPRTQAFLSKVLH"
     gene            complement(5184..5864)
                     /locus_tag="AC16FU_04115"
     CDS             complement(5184..5864)
                     /locus_tag="AC16FU_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR2993590.1"
                     /translation="MLDWLAAELTILKNIILFLLPAVPATIEVTLIAFALALVLGQLI
                     GLLRISRRKVFGITSKIYVDTIRGIPLLVQIFFIYFGLGKVLNLDRFLAGVVAIGVCY
                     SGYLAETFRAGIQAIHKGQYEAALSLGLTRWQMMRHVILPQSLRIVIPPVANDFIACL
                     KDSSLVSVIGLRELTRAGRDYYSQFFVDFQTWLIVGLLYLVMTLTLSRFVAFLEKKFA
                     VHGFEVVH"
     gene            6082..>7830
                     /gene="aspS"
                     /locus_tag="AC16FU_04120"
     CDS             6082..>7830
                     /gene="aspS"
                     /locus_tag="AC16FU_04120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR2993591.1"
                     /translation="MADSVSSQTQPLTPTSPQPVIWTSDWKRTHTCGQLRQADGGKKV
                     TLNGWVYRWRNHGGLAFIDLRDRYGITQVVFNPKILGQEAVDRAGSFRAEYVIAVKGT
                     AAIRPTGMQNPQLETGEIEVAVESFAVLNESKTPPFEIADDSDATEELRLKYRYLDLR
                     RKPLQENIRLRHRVAKAVRDYFDSVGFYEIETPVLIRSTPEGARDYIVPSRVNRGKFY
                     ALPQSPQLLKQILMISGFDRYVQLARCLRDEDLRADRQPEHTQIDLEMAFVTQDDVFS
                     VVEGMFKHIFKVVRNEELPTPFPRIRYVDAMNRYGSDKPDLRFGMELVDLTDLVQQSP
                     FRVFSETVKNKGVVKGLAVKGQAAMTRSQIDHWEETARKWGAKGLIWIVRTKDEVRSP
                     LTKFVPKEEIDTLVKRLSLEIGDLAFLVADRWHVTCNVLGRLRVELARQLGLIPQDQW
                     NFSFVIEFPLFEYHPQEKRYNAMHNIVTSPWAEVIPKLDAGFTSPLSLDHPDHPWGQI
                     RANQYDLVLNGNEMASGGIRNHRRDIQQKILNVLGMSDERAERMFGFLLTALEYGAPP
                     HGGIALGLDRIVTLLTG"
BASE COUNT         1791 a         2021 c         2244 g         1774 t
ORIGIN      
        1 aaattgcctt ccacgataaa aacgtgcccg gaatactcca agccaaatac tttaatcctc
       61 aaattctttg cctgcgggtc aaaaattatg agcattagat ttgggttttc agtcaaagca
      121 aattttatcc ggcttaagac atcatagtcc cgaaaggagt aacctatgaa aacggccaca
      181 tttgtattta gcagacaatg atggagatag ttgtatgcag taaggaacgg ctcggctatg
      241 gccacctttt tcatggcagg atagataatg acgtttctaa taatatcatc gtctccatga
      301 tgttccgaaa atggtacccg aacgattttt ccatttattc tgtaccaatc gactgaaccg
      361 tgtaatttaa agagacagat ctgttttttc cgaggatctg ggttgaattc aaatcctctt
      421 ctatcccaga caaattcttg agaattgaag tcctgaatga tgccattgca aaaatttata
      481 tttcgttgcc agaaatttcc aaacttgcaa gcttcttcaa tagcggggtc gtagttcgta
      541 gtgaaaatag gcaaaatttc tgtatctttc gtggagttta atagttggaa agtgaagtgc
      601 gaatgttcta gtacccattt caggtattcc gctgcatcac cagcaataat cgggctgtaa
      661 tgcttaaaaa tttccccttt gagcttaaga gacaaagatt cagcaggttg agtataacca
      721 gaaagagcgg gtaaatactc tttggaggaa atctcttcca gttcttgtaa gaggaattct
      781 agatcatatt ctttcttaag cattttttcc aacagttcgc gttccatgta ggggaaattt
      841 tgatcgtttc gcacttgaat cgaaaagtcg tccacgaaat ctctcatcaa gcgttttcct
      901 aagcattttg tagctcccgc acccaaaaag aaaattaggg gtgcatccgt tatgagccta
      961 ttcatggtca acgagcattg ttagaacttc gccgcatacg ccagcaccca gcggaacttc
     1021 catctgctct cgcccggcag aggatcggag atccagagcg ggaaataaac gcggatgtag
     1081 ttgtcgccca ccagccgccg gatactctgg tacgggaagc ggtaggaaag cgaaagtccg
     1141 agatcagaga aaaagtcacc gagatcaggg atgttgccgg aattccaccc ctggccaaag
     1201 tcgaagaagg cgtgggtttc tatggaggtg aggtcgcgcc agaggatttc gctgccttgg
     1261 tggattttgg tcaacagttt cgtcagcgga ttctcgaaac cgagccggac attggcgctg
     1321 gcgatgcgtt tgtacccggc attctttttg agaaagccgc gcaggttgcc cccgccggga
     1381 aggtagaggt ttccggttcg cttcaagcct tcgggcaagg agccgcggct ggagtaccac
     1441 cggctgccga actgctcatc gggactggcg gaggccacgt agaagaggtc ctgcaggggc
     1501 acgtcgcctt cggcataccc gaaatagagc cgggaccaga gcgggaattt gtcgggggta
     1561 tgccagtcac tttctatttc cagggcggcc ttgccaaagt cgtgttgcgt gcggagggcc
     1621 gcggaagtca gctccagcgc aatgcggctg tcgaacttcc acctgttgaa cacggcattc
     1681 caggaaaact tccaggtcga gacccgtccg gacgaccagg actgggtgcg ggagaggtag
     1741 tcgggatggt ccagccgcga gtaattccac gacaaactga cgcggcgcgg gggtgcggcg
     1801 gtaaagccgc cgaagccgaa aaagccgctc cgtccgtagg tgcgcgacag agaaagtgag
     1861 gccccctgcc ggccttcgag aaatgagccg ccggctgacc aggttgtccg gttgtcgaac
     1921 aggtagaagg gcgaagtgta gttgacgttg aaatcgacct gcgcatggcg gacgccgacc
     1981 cagaggtcgg tattcaaccg gtgatcgaca cctaagtatg aaccgaagcc gccaaaccct
     2041 aaacgagcca cgtcctcctg gttgtaccag agtttcggct ggtaacggat ctggtaggca
     2101 tccagaggcg ggtcgggcga gtagcccgga aagagaaatt gcggcggctg aaattggaac
     2161 gtgaccggag cccgcgggtg gtagtcgttc aagcggttga catcctgcac ccgcaggtcg
     2221 gggtttaaga ccgcccgctt gaccggagaa gagacgggga tggtaatcgt ttgtaacgtc
     2281 ggtcccttca tccagagatc gaacggaatg tcccggctga ccttctggcc gtcggccagc
     2341 gtcaactgaa cgtccaacgg cataatggcc cggccccggc gtttgatttt cacctccgcg
     2401 tcataaggag caggacgatt cggatctttc agttcggatt tgactccttc gaggctgtag
     2461 tcgcaggtgt aaacccgctc gaaccactcc tgaaagaacc attctaagtg acaaccgctc
     2521 gatttttcga tggaggcgta aaaatcctct ttgtggggat gtttataatg ccagcggtcg
     2581 tagtagtcgt gcatcgcttt ggcaaaaacc gaatctccca agacatactg gagcatgaag
     2641 aggatggaag cggtcttggg gtaaacggcg ttggtgtacg agccgggcag gaagcggtcg
     2701 gagtgggtgt cgatgacatc ctcggttccc gtcttggcga agcggaggta agaaaagaag
     2761 gtgtctttgc gctcgtcgcc attgggatag aattttttct ggtaccactt ctcccaggtg
     2821 tagttgttgt cgtagcgccc gtagtagtgc tccatggcca ggcaagtagc aaaggaggtg
     2881 aagccctcgt ccaaaaacgc ctggcgggtt tcgttgttgc cgagcaggcc gtagaaccag
     2941 ttgtggccga tttcgtgggc cagaatcgca aagagcccgt gactgtattt cgacccgctg
     3001 cgccgggtga taatcgtcat ctgtgggtac tcgatgccgc cgccgacgga accttccacc
     3061 gacgaaaact gcgggtaggg ccacatcccg aaggtctggc tgtagaactt gatgatctcc
     3121 cgggtgtaaa tcgcgcccca tttttgccag aattcctgct tatccttctg gtagagggcg
     3181 ttcatcacaa tgccttccca ctgagcgcgg tcccagatga aatcggggtc ggcggcgaag
     3241 gcgaaatcga agacggtgtc ggcgtggcac ttccaggtcc aggttttggg ctcgttcttt
     3301 ttcccggcag tatcaagtgg ggacgaatca ccgtttggat tctgcatttc ggggtgtcgc
     3361 ggttcggcac gcttaccgcc ccgagtcggc ctttggcgga tcgaagggga ggccgaggtg
     3421 tcggggttgg tggaatcgaa ggcgatttgc ttctcggacg ggtgctgctc tttatagagt
     3481 ttcagaatat caatgacaac cgaatcacct tcggccttcg gcaggccgcc ggggaagagt
     3541 tcctcttcgt ttgtgagttt tccggtgtgg gcgaggacat actctttcgg taccgttatc
     3601 gacacgtcga aggtgccgta gtcggcgtag aactcgctcc ctaagtactg gtcgtcatgc
     3661 cagccgcgtt tgtcgtacgc cgccaggcgt gggaaccact ggccgatgtc gtagtgccgc
     3721 ccctgcttgc ccatccggga gccgttgcgg gggattttgt cgacgaactt gaaaaagaaa
     3781 gcgatttttt caccggacaa aagaggtttg ggcagatcga ctttcaaaat cgtgttgtct
     3841 aattcgcttt tcgtccaccg ttcaccctca attgccagag aatcaatccg ctcttcgccc
     3901 cagtcgcgct tggggaggtc gcgcagggag aaattgccgg cttcccggct gcgcaggtcc
     3961 atatagctcc ccggctggaa ggcgttcagg tagaggtgaa agtaaacgta agtgagcgtg
     4021 tcgggcgagt ggttcgtgta gacgagcgtt tcggtgccgg tgagggagtc gcctttcggg
     4081 aacaactggc agtcgatggt gtaagccact tcttgctgcc agtaggagtc agcctgactt
     4141 gcgctgaccc tcactatgcc gaatccaagg aaaagaaaca gagcgcgttt gagcatcatt
     4201 gacctccagt tgggttggaa tgggaaaacg ggaatcgcag ccgatgaagc ggatgtcctg
     4261 gtcagtgtct gtttcatttg ctccatcggc tgtgaatgct ttgtagtttt caatgcagca
     4321 ccttgctcaa aaaagcctgc gtacgcggat tttcgggagc ccgcagcact ttctcgaccg
     4381 gcccgatctc gaccaacttg ccttcatcca gaaaggcgat ccggtgggcc acttcccgcg
     4441 caaagcccat ttcgtgggtc accacgacca tcgtcattcc ttcccgggcc aactttttca
     4501 tcacacccaa aacctcgccg atcatttccg ggtccaaggc cgaggtcggc tcgtcaaaga
     4561 gcattatctt gggattggta gccagggcgc gcgcaatggc cacccgctgc tgctggccgc
     4621 cggagagctg cccggggtaa gcggccttct tctccagcaa ccccactttt tccaagagag
     4681 tttccgcaat tttcccggct tcagctttcg tgcgcttgcg caccacccgt tgggccagat
     4741 agatattctc caaaacgctc aagtgcggga aaaggttgaa ctgctggaag accatgccgg
     4801 tttcgcgccg gatggcgtag atgtgggctt tcttgtgatc cagcatgatt ccatcaacga
     4861 ttacctcgcc ggagtccggg atttcgagcg cgttcatgca gcgcaaaagc gtgctcttcc
     4921 ccgatccgga cgggccgacc aaaacgatca cctcgccggg ctcgactgaa agagatacgc
     4981 catccaaggc caccacttgg ttgaagcgct tagagacgtt tttgagtgcg atgatggaca
     5041 ttatgccgac ttttaccgta gcgcgggatg gttttcgtat cccgcgcttg attgactcag
     5101 aggcggcgca agtttccaaa accaacctgc gctacgaatg ggagaatcgt gcatctgctt
     5161 catcttctgc tgtgaattct tatttagtgc accacctcaa acccgtgcac ggcaaatttc
     5221 ttctccagaa acgccacaaa tcgtgacagg gtgagtgtca tcactaaata taacagcccg
     5281 acgatgagcc aggtctggaa atcgacgaag aactgggaat agtaatcccg cccggcgcga
     5341 gtcagttcgc gcaggccgat caccgacacg agtgaactat ccttgaggca ggcgatgaag
     5401 tcgttggcca ccggcggaat aacgatgcgc agcgactggg gcaggatgac atggcgcatc
     5461 atctgccagc gggtgaggcc caacgacaag gccgcttcgt actggccttt gtggatggcc
     5521 tggattccgg cgcggaaggt ttcggccagg tagccggagt aacagacgcc gatggcgact
     5581 accccagcca gaaagcggtc caaattcagc actttgccca ggccgaagta gatgaagaaa
     5641 atctgcacca aaagtgggat gccgcggatg gtatcgacgt agatttttga cgttatcccg
     5701 aaaactttgc gccgggagat gcgcaaaagg ccgatgagtt gtcccagaac caaggccagg
     5761 gcaaaagcaa tcagcgtgac ttcaattgtg gccgggacgg cgggtaaaag gaaaaggatg
     5821 atgtttttca gaatagtcag ttcggcggca agccaatcga gcataggtgc tgactggtca
     5881 aagaaaggaa aagttgggta gtgtcaagcc tcctcactta ttctgaagag tggatttacg
     5941 tggcgagggc gccgcggcta cggttttata taacttcttc tcacttgacc gataagatta
     6001 ctatggctct tgcggcaaca aacggttttt gtagagcgtt tgacttctag ctcgtcgggc
     6061 attattctta aactgaaacc tgtggcagat tccgtaagta gtcaaaccca acccctgacg
     6121 cctacctctc cccagccggt catctggact tctgactgga agcgcacgca cacctgcggc
     6181 cagctgcggc aggcggacgg cggcaaaaaa gtgaccctga acggctgggt ctaccgctgg
     6241 cgcaaccacg gcggcttggc cttcatcgac ctgcgggacc gctacggcat cactcaggtc
     6301 gtcttcaacc ccaaaattct ggggcaggag gctgtcgatc gggccgggag tttccgggcg
     6361 gagtacgtca tcgcagtgaa aggcacggct gccattcggc caacggggat gcagaacccg
     6421 caactagaaa cgggcgaaat tgaggtggcg gttgagtcct ttgccgtttt gaatgagtcg
     6481 aaaacgccgc cctttgaaat tgcagatgac tcggacgcca ccgaagaact gcggctaaag
     6541 taccgctatt tagacctcag gcgaaaaccg ctgcaggaga atatccgctt gcggcaccgg
     6601 gtggccaaag cggtacggga ctatttcgac tcggtcgggt tctacgaaat cgaaacgccg
     6661 gtattaatcc gctccacgcc ggagggagcg cgggactaca tcgtaccgtc gcgggtcaat
     6721 cgcggcaagt tctatgccct gccgcagtcg ccgcagctgc tcaagcagat tttgatgatt
     6781 tccggcttcg accgctacgt gcagttggcc cgctgcctgc gcgacgaaga cctgcgggcc
     6841 gaccgccagc cggagcacac gcaaatcgac ctcgaaatgg cctttgtgac ccaggatgac
     6901 gttttttccg tcgtggaagg gatgttcaag cacattttca aagtagtccg caacgaggag
     6961 ttgccgacac ccttcccgcg catccgctac gttgacgcga tgaaccgcta tggctccgat
     7021 aagcccgatt tgcgcttcgg gatggagttg gtggatctca ctgacttagt ccagcagtca
     7081 ccctttcgcg ttttctcgga aacggtaaaa aataagggcg tcgtgaaagg gctggcagtc
     7141 aaaggccagg cggcaatgac gcgcagccag attgaccact gggaggagac agctcggaag
     7201 tggggagcca agggattgat ctggattgtt agaaccaaag atgaagttcg ttcgcctttg
     7261 acgaaattcg tcccaaaaga agaaatagat acgctcgtga agcgattgag tctggagatt
     7321 ggcgacctgg ctttcctcgt ggccgaccgc tggcacgtca cctgtaatgt cctggggcgg
     7381 ctgcgcgtgg aattagcccg gcagttgggc ttgattccgc aagaccagtg gaatttctct
     7441 ttcgtgatag aatttccgct cttcgaatat cacccgcaag agaagcgcta taacgcaatg
     7501 cacaacatcg tcacttcccc gtgggcggaa gttattccga aattagatgc cggtttcaca
     7561 tctcctttgt ctcttgacca ccccgaccac ccctggggac aaattcgtgc caaccagtat
     7621 gatctggtgc tcaacggcaa cgaaatggcc tccggcggga ttcgcaacca ccggcgcgac
     7681 attcaacaga agatattgaa cgtgttaggg atgagtgacg aacgggctga gcggatgttc
     7741 gggtttcttt tgacggctct cgaatacggc gcgccgccgc acggcggaat tgcgttgggg
     7801 cttgatcgga tcgtgacgct gcttaccggt
//
LOCUS       DBNGZA010000197         2336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_142730, whole genome shotgun sequence.
ACCESSION   DBNGZA010000197 DBNGZA010000000
VERSION     DBNGZA010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_142730"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..778)
                     /locus_tag="AC16FU_04125"
     CDS             complement(41..778)
                     /locus_tag="AC16FU_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR2993592.1"
                     /translation="MEGQATVMPATQEPAMNLLARIWNVWVAPKKAFANVKLYPRWVV
                     PFFLLLAIHFAVRPVVAPQVAKDAEQIVNTSPRFENVPAEQKEKMIAGFHQGLTVKNV
                     IQGVLISTIITVLMGALFLFFTNVLLGGQVKYWQTTAVFIYSGFILIPEYVLKLPLIF
                     AKKSALVATGPGLFVSAGEGFGAHFLSAIDFFGLWQVGVVAVGLRVLAGSTTKKASVW
                     IFSAWVLWVIIKAALSYFSGGLSRLSF"
     gene            complement(1036..>2336)
                     /locus_tag="AC16FU_04130"
     CDS             complement(1036..>2336)
                     /locus_tag="AC16FU_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0098655 - monoatomic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proton/sodium-translocating pyrophosphatase"
                     /protein_id="HIR2993593.1"
                     /translation="VSAVTATLGFGLINYFFIKTPDGSADFRFFWATFSGIILAVVIS
                     YLTEYYTALEYSPVKETARATKTGPATTILAGFSEGLESTVVAIMAIAVAIVASALIF
                     KGDLALAAYGIALCGMGMLTNTGVIVSMDSYGPISDNANGIFEMSKVLEEDKNPKSHM
                     IVAKLDAVGNTTKAVTKGFAIASAVVAAVALFRSFSSDVNILDPALNFISKGIQVNLP
                     YVFVGLLIGGAMPFLFCSVAIRAVGRAAFQVVEEVRRQFREHPGIMKGTEKPEYYRCV
                     DIATSAAQKELITPGILAVSAPVAVGFLLGVEALGGFLAGIILTGQLLAVLLSNAGGT
                     WDNAKKSVEAGLYGGKGSEAHKAGIIGDTVGDPFKDTAGPSLNPLIKVMNLVSLLILP
                     LIVGRGKLGGTVATVTAVGCLVLLVISLLISKRSTQAKTA"
BASE COUNT          523 a          712 c          662 g          439 t
ORIGIN      
        1 aaaatcgtct tcctacgcat tctcctcctc gtttatgcaa ctaaaaagat agccgggaca
       61 gtcctccgga gaaataactc aaggcggcct taataatcac ccagagcacc caggcgctga
      121 agatccacac gctagccttc ttggtcgtgc tcccggccag cactctcaag ccgacggcaa
      181 cgaccccgac ctgccagaga ccgaagaaat caatggccga aaggaagtgc gcgccgaacc
      241 cctcccccgc cgacacaaag agccccggcc cggttgccac cagggcactc tttttggcga
      301 atatcaacgg caacttcagc acatactcgg gaatgagaat gaatccagag tagatgaaga
      361 ccgccgtcgt ctgccagtac ttcacctggc cgcctaaaag gacattggtg aagaagagga
      421 acaaagcacc catcagcacg gtgatgatgg tcgatatcag cacaccctgg attacgttct
      481 tcactgtcaa accctggtgg aagccggcga tcattttctc tttctgctct gccggcacat
      541 tttcaaagcg cggcgaggta ttcacaatct gctccgcgtc tttggccacc tggggagcca
      601 ccaccggccg gacggcaaag tggatcgcca gcaaaaggaa aaacggcacc acccagcgcg
      661 ggtagagctt gacgtttgcg aatgcttttt tcggcgccac ccagacgttc cagatgcgcg
      721 ccaaaaggtt catcgccggt tcctgggtcg ccggcatcac ggtcgcttgt ccttccattc
      781 gctctccttt gcctccgtgt taggttgcag aacgtaagaa acgaaaagaa gtttcaaaaa
      841 tcaaaccggg cgttctctct ctaaaggtgg ggatcagaat agggatttcg ttctctccca
      901 ttgaatagga cgaggtggca gcgcaagcgg cgaccgaggg cggtcgccct acgcgagggg
      961 atggctgatc acccgcacgg tgatataaca tccgaccgcg tgggcgcggc gccccccgcc
     1021 gcgctgaaaa gtttgctatg cggttttcgc ctgcgtactg cgcttactga tcagaagcga
     1081 gatgaccagg agaaccaagc agccgacggc ggtcacagtc gcaaccgtcc cgcccagttt
     1141 cccccggccg acgatcagag ggagaatcaa aagcgagact aagttcatta ccttgatcaa
     1201 ggggttgagc gacggcccgg cggtgtcttt gaacgggtcg cccaccgtgt cgccgatgat
     1261 tccggctttg tgcgcttcgg agcccttgcc gccgtagagc cccgcctcca ccgatttttt
     1321 ggcattgtcc caggtcccgc ccgcgttgga gaggagcacc gccaacaact gtccggtgag
     1381 aatgatcccg gccagaaatc cgcccagcgc ttccacaccc agaaggaagc cgaccgccac
     1441 gggcgcggaa accgccagga ttccgggagt aatcaactcc ttctgcgctg cggaagtcgc
     1501 gatgtcaacg cagcggtagt attccggttt ttccgttccc ttcataattc ccggatgctc
     1561 ccggaactgc cgccgcactt cttccacgac ctgaaaggcg gcgcggccca cggcgcggat
     1621 cgccaccgaa cagaagagga acggcatcgc tccgccgatc agaagcccca caaagacgta
     1681 gggcaggttc acctggatgc ccttgctgat gaaattgagc gccgggtcga ggatgttcac
     1741 gtccgaagag aaggagcgga agagcgccac cgccgccacc accgccgagg cgatggcgaa
     1801 ccctttggtc acggccttgg tcgtgttgcc caccgcgtcc agcttggcca cgatcatatg
     1861 cgacttggga tttttgtcct cttcaaggac tttagacatt tcgaagatgc cgttggcatt
     1921 gtctgaaatc ggcccgtagg aatccatcga gacgatgacg ccggtattgg tgagcatccc
     1981 catcccgcag agcgcaatcc cgtaggccgc cagagccagg tcgcctttga agatgagggc
     2041 cgaggccaca atcgccaccg caatggccat gatcgccact accgtcgatt ccagcccttc
     2101 ggaaaaaccg gccagaatgg tggtggccgg tccggtcttg gtcgcccgcg ccgtctcttt
     2161 caccggactg tactcaaggg cggtgtagta ttcggtcagg taggaaataa cgacggccag
     2221 gatgatgccg gagaaggtgg cccagaagaa gcggaagtcg gccgacccgt ccggggtttt
     2281 gatgaagaag tagttgatga gtccgaagcc gagggtggcg gtcaccgccg agacca
//
LOCUS       DBNGZA010000198         1390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2158156, whole genome shotgun sequence.
ACCESSION   DBNGZA010000198 DBNGZA010000000
VERSION     DBNGZA010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2158156"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="AC16FU_04135"
     CDS             complement(<1..98)
                     /locus_tag="AC16FU_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR2993594.1"
                     /translation="MGLKVVFVSSEAVPFAKTGGLADVSGALPKALA"
     gene            complement(253..558)
                     /locus_tag="AC16FU_04140"
     CDS             complement(253..558)
                     /locus_tag="AC16FU_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993595.1"
                     /translation="MSQGLITLRSGGGDRSHLIATGPFDLFPGDSVKVAFAVVGGATF
                     AQLQANADSAKVRYLRMNSSVPLLTPQGLAGLAALLLAALTFGFSRWQRRRTATTLG"
     gene            complement(565..>1390)
                     /locus_tag="AC16FU_04145"
     CDS             complement(565..>1390)
                     /locus_tag="AC16FU_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993596.1"
                     /translation="DSAANSGTLLRFAFDPTAPPNTNLPLQLTVFSNAGPGDTLPLFF
                     HTGDRLVRSIVNHSLANNFFTVSNFGQYGFATGSLNPRSPGQGFFYPKVGGANNLFEM
                     ALVVGMSDSTVSNAARGQPSDLIDRDFAPASEGNLIRQAPGPRAAEETLCRFNDSGAV
                     RPLGLEINQHTFAFSDTANDDYVIVEYVLKNASGVSRMGVRIGLFADWDFPYGSGASD
                     SVNFDASRNLGYMFDGGNPSARKPRGVAVLTLPGATAYRAINSPTEIYDGFSQAEK"
BASE COUNT          313 a          398 c          421 g          258 t
ORIGIN      
        1 gccagggcct tcggcagggc gccggaaacg tcggccaacc cgccggtttt ggcgaacggc
       61 accgcctcgc ttgaaacgaa aacaactttt agccccatgg atgctcctct atttttcgtg
      121 aaatctctca gtcaacaacg tcgtacgctg ccttactcca tgcccagtaa tctatactgt
      181 gcaaacggat ggtcaagaac aatctgttgc ccgtgggaac ggcgctggag ggaagctatt
      241 ggaactagaa ggtcagccaa gagttgtcgc agtacgcctc cgctgccagc gggagaaacc
      301 gaaggtgagg gcggccaaaa gcagcgctgc caaaccggcc aaaccctggg gtgtgaggag
      361 gggcaccgac gagttcatcc tcagatagcg aaccttggcc gaatcggcat tggcttgcaa
      421 ctgggcgaag gtcgcaccgc ccacgacggc gaaagccact ttgaccgaat caccggggaa
      481 tagatcaaac gggccggtgg caatcaggtg ggagcggtca ccccctcccg agcggagcgt
      541 aatcaaaccc tgactcattc cctgtcactt ttccgcctga gaaaatccgt cgtaaatctc
      601 ggtcgggctg ttaatggccc ggtaggcggt cgctcccggg agagtaagga ccgccacccc
      661 gcgcggcttg cgggcggaag ggtttccccc gtcgaacatg taacccaggt tgcggctggc
      721 atcgaaattg accgaatcgc tggcgcccga accgtaggga aaatcccagt cggcaaagag
      781 gccgatgcgt acacccatcc gtgagacacc ggaggcattt ttcaggacgt actccacgat
      841 gacgtagtcg tcattggccg tgtcagaaaa agcaaaggtg tgctgattaa tttccaatcc
      901 caacggccgc acggctccgc tgtcattgaa tcggcataac gtttcctcgg ccgcccgcgg
      961 ccccggagcc tggcggatca gattcccctc agaagccggt gcaaaatcgc ggtcgatgag
     1021 atcggagggt tgcccacgcg cggcgttcga gacggtgctg tcgctcatgc ccaccacgag
     1081 cgccatttcg aagaggttat tcgctccgcc gactttgggg tagaagaatc cctggcccgg
     1141 actccgcggg ttgagtgaac cggtagcgaa gccgtactgt ccgaaattcg agacggtgaa
     1201 gaaattgttg gccagtgagt ggtttacaat tgagcgcacc agccggtccc cggtatggaa
     1261 gaacaaaggc agggtatccc ctggcccggc gttcgagaaa acggtcagtt gcaagggaag
     1321 atttgtattc ggcggtgcgg tcgggtcaaa agcgaagcgc aaaagcgttc ccgaattggc
     1381 cgcgctgtct
//
LOCUS       DBNGZA010000199         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1949363, whole genome shotgun sequence.
ACCESSION   DBNGZA010000199 DBNGZA010000000
VERSION     DBNGZA010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1949363"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="tig"
                     /locus_tag="AC16FU_04150"
     CDS             <1..1245
                     /gene="tig"
                     /locus_tag="AC16FU_04150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR2993597.1"
                     /translation="AQFAATYEQFKAKAKIPGFRPGKAPLDLVKERFAGLARQEVLEE
                     LVPQAYQEAVAQTDLHPITEPEVKDVQFSNGQPLRFAAEFEIRPVAELKKYTGFKFSK
                     PKYEITDADVTEFLTRQRERKAQLKPVARAAASGDVVVATIEKLSDRENRIPPFKKEN
                     AAIDLEQTRDLDDFAKEIVGMRVGEIKEFDVNYPADFSEQAYAGNRVRYWVLAKEVKE
                     KILPPLDDEFAKQEGFTALADMREKVQQTLTAEMEREAESRLRAQVRHTVVEQNHFPV
                     PQSLLATYLDGVAADFRKNYQNFDEKQVRESYRPLGEELIQWEILLRQIAEKENLAPT
                     KEDTNAWLSRFAARYQITPEEAQETLAKSGKIKDVRETILEQKVIDHILKHSEVSETP
                     VAKETASGAKQTGKEKTSKLIT"
     gene            1263..1907
                     /gene="clpP"
                     /locus_tag="AC16FU_04155"
     CDS             1263..1907
                     /gene="clpP"
                     /locus_tag="AC16FU_04155"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR2993598.1"
                     /translation="MKKIIIRSDEEDDDNERRQMTLIPMVVEQTGRGERAYDIYSRLL
                     KDRIIFIGTPVDDTIAKLVIAQLLFLEAEDPEKDIQMYINTPGGIVSAGLAIYDTMQF
                     VKPPVATTCVGMAASMGALLLAAGTKGKRAALPHSRVMIHQPWGGVQGQVTDIQIHAK
                     ELLLTRSRLNEILAKHTGQPLEKIEKDTDRNYFMSAEESKEYGIIDQVFERKPK"
     gene            1909..>2489
                     /locus_tag="AC16FU_04160"
     CDS             1909..>2489
                     /locus_tag="AC16FU_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993599.1"
                     /translation="MRKLTATVALLFLAGQAAAQDTAADRFRKVALRLVEAINAGDSL
                     GIQRDFGQVMLEGFPPEKSIPFFRELTGQLGKIQKLDSLRLVPPNQAIFPAHFERGLL
                     DLKIVLDQEDKIIGLWFLPHAPDIPVPDKHATELSLPFKGRWLAFWGGDTKELNQHHD
                     VPNQRFAFDFVGVGADGQTHRGDGKKNEDYYAFG"
BASE COUNT          624 a          689 c          747 g          429 t
ORIGIN      
        1 gcccagtttg cggccaccta cgagcagttc aaagccaaag ccaaaatccc cggcttccgg
       61 cccggcaaag cgccgttgga tttggtcaaa gaacgcttcg ccgggctggc ccggcaggaa
      121 gtgttggaag aactggtgcc tcaggcctac caggaagcgg tggcgcagac tgacctccac
      181 cccatcaccg agccggaggt gaaagacgtt cagttctcca acggccagcc cctgcgcttt
      241 gcggcggagt ttgagattcg cccggtggct gaactgaaaa agtacaccgg cttcaaattc
      301 agcaagccga agtacgagat caccgatgcg gacgttaccg aatttctgac ccgccaacgg
      361 gaaaggaaag cccaactcaa gccggtggcg cgggccgcgg cctccggaga cgtggtcgtc
      421 gccacaattg agaaactctc cgaccgggag aaccggatcc cgccgttcaa aaaggagaat
      481 gcggcgattg atttagaaca gaccagggac ttggacgatt ttgccaaaga aatagtcggg
      541 atgcgggtgg gggaaatcaa agagtttgac gtgaactacc ccgccgattt cagcgaacag
      601 gcatatgcgg gcaaccgggt ccgctactgg gtgctggcga aagaggtcaa ggaaaaaatc
      661 ctgccgcctc tggatgacga gtttgccaag caggaggggt tcaccgcgct tgcggatatg
      721 cgggagaaag tacagcaaac gctgacggcc gagatggagc gcgaggccga atcccgcctc
      781 cgggcgcagg tgcggcacac ggtggtggag caaaatcact ttcccgtacc gcaatcgctt
      841 ctcgccacct atcttgacgg cgtggcggcc gatttccgga agaattatca gaacttcgat
      901 gaaaaacagg tgcgggagag ttaccggcca ttgggcgaag agctgattca atgggaaatt
      961 ctgttgcgcc agatcgccga aaaggaaaat ctcgcgccga cgaaagaaga taccaacgcc
     1021 tggctttcac gctttgccgc ccgctaccag atcacgcccg aagaagcgca ggagacttta
     1081 gccaaaagcg gcaaaataaa agacgtgcgg gagacgatct tagagcagaa agtcatcgac
     1141 cacatattga aacactccga agtgtcggag acgcccgtcg caaaagaaac ggcaagcgga
     1201 gcgaagcaga cgggaaaaga gaaaacaagt aaattgatca cgtaaacgca ggcaggtcaa
     1261 acatgaaaaa gatcattatc cgatcagacg aagaggatga cgacaatgag aggagacaga
     1321 tgaccttgat tccgatggtg gtggagcaga ccgggcgggg tgagcgggcg tacgacatct
     1381 actcccggct gctcaaggac cggatcattt tcatcggcac gccggtggac gacacgatcg
     1441 ccaagttagt gatcgcgcag ctcctctttc tggaagccga agacccggag aaagacatcc
     1501 agatgtacat caacacgccg ggcgggatcg tctccgcggg cctggccatc tacgacacga
     1561 tgcagttcgt caagccgccg gtggcgacga cctgtgtggg gatggcggcc tcgatgggcg
     1621 cgctcctttt ggccgccggc accaagggga agcgggcggc cttgccccac tcgcgggtta
     1681 tgatccacca gccctggggc ggggtgcagg ggcaggtgac cgacatccag atccacgcca
     1741 aagaactttt gctgactcgc tcgcggctga atgaaatttt agccaaacac accggccagc
     1801 cgctggaaaa aatcgaaaaa gacaccgacc ggaactactt catgtcggcg gaagaatcga
     1861 aggagtacgg gatcatcgac caggtctttg agcggaagcc gaaatgaaat gcggaaactg
     1921 accgcgacag ttgcgctcct ctttctcgcc ggacaggcag ccgcacagga caccgctgct
     1981 gacaggtttc gtaaagtggc tctccggctg gtggaggcga tcaacgcggg tgattctttg
     2041 ggcatccagc gtgacttcgg tcaggtaatg ctggaggggt tccccccgga gaaatcgatt
     2101 ccgttcttca gggaactcac cgggcagttg gggaagattc aaaagctcga ttccctccgg
     2161 ctcgttccgc ccaaccaggc gattttccca gcccactttg aacgggggct tttggatctg
     2221 aaaatcgtcc tcgaccagga ggataaaatc atcgggctct ggtttttgcc gcacgcgccg
     2281 gacatccctg ttccggacaa gcacgcaaca gaactctccc ttcccttcaa gggccgctgg
     2341 ctggcattct ggggcggcga caccaaagag ctcaaccagc accacgacgt accgaatcag
     2401 cgcttcgctt tcgatttcgt tggcgtcggt gcggacggac agacacaccg cggtgacggc
     2461 aaaaagaacg aagactacta cgctttcgg
//
LOCUS       DBNGZA010000200         1952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2265350, whole genome shotgun sequence.
ACCESSION   DBNGZA010000200 DBNGZA010000000
VERSION     DBNGZA010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2265350"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1033
                     /locus_tag="AC16FU_04165"
     CDS             89..1033
                     /locus_tag="AC16FU_04165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR2993600.1"
                     /translation="MHSPPAGPFGNGHTLHRVYFNGAFVPAEQAKVSIQTHAFNYGTA
                     IFEGMRAYWNAEKKRLFGFRWEAHFRRMEAGCRVLKIKLPHHAADLINLTKELLRAND
                     CREDIYIRPLAYKAREGLGPRLTEQDGFAIYLVPFPSVPNPRPWSVCISSYRRIPGFA
                     VPAGVKITGAYVNAALATTEAKEQGFDEAILLDKDGFVCEGSAMNLFLVTDGKLVTPP
                     LTGDILGGVTRDTIITFANEELRIPVEECPVAPEQLFAAEEVFFCGTGVEVVPISSVG
                     RRAIGDGKPGGVTGRLAALYLQVVRGEKEKYQKWLVEI"
     gene            1055..1480
                     /locus_tag="AC16FU_04170"
     CDS             1055..1480
                     /locus_tag="AC16FU_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR2993601.1"
                     /translation="MRWVTREKVKVDRVACPWLIKEFIDPEAEFVFVPRDTDPASIRD
                     GIPFDMAGVELGHREGRCSFESILLKYGLSDPALHEMAKIIHAADIAADEDLVPEGKG
                     LKAIATGFSLLGLSDHQILEKEFIVYDALYRFCQEKVHK"
     gene            complement(1593..>1952)
                     /locus_tag="AC16FU_04175"
     CDS             complement(1593..>1952)
                     /locus_tag="AC16FU_04175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR2993602.1"
                     /translation="VAFYPADWSGGCTKQVCSYRDNFSAFTDLNCEVIMISGDYVFSH
                     QEWAKHHNLPFILASDHDHSVAKTYGSFMPEMGFNKRTVFLVDKAGIIRYINLKYNPG
                     TPDDFEKLKAEAKKLAG"
BASE COUNT          403 a          518 c          622 g          409 t
ORIGIN      
        1 gtggtcgatg atttcggacg cacgctgaag cgatacgcgc gggcgctgcg gagcaggaat
       61 gagagaagat tgcgtcggag cataaagcat gcattcaccg ccagccggtc cattcggtaa
      121 cgggcatacg ctgcaccggg tctatttcaa cggagcattt gtccccgccg aacaggcgaa
      181 ggtttccatc cagacgcacg ccttcaacta cgggacggca atcttcgaag ggatgcgggc
      241 ctactggaac gctgaaaaaa agagactatt cggcttccgc tgggaggcgc acttccggcg
      301 gatggaggcg ggctgccggg tgctgaagat caagctgccc caccacgccg ccgacctgat
      361 caacctgacg aaggaactct tgcgcgccaa cgactgccgg gaggacatct atatccggcc
      421 gctggcctat aaagcgcggg aggggctggg gccgcgcttg accgaacagg acggttttgc
      481 catctacctc gtgccgtttc cctcggttcc caacccgcgc ccctggtcgg tttgcatttc
      541 ctcttaccgc cggatacccg gctttgcggt gccggcgggg gtaaaaatca ccggcgcgta
      601 cgtcaacgcc gctctggcga ccaccgaagc caaggagcag ggttttgacg aggcgattct
      661 cctggacaag gacggcttcg tctgcgaggg ctcagcgatg aatctcttcc tggtcaccga
      721 cgggaaattg gtgacgcccc ctctgaccgg cgatattctg ggaggcgtga cgcgggacac
      781 gatcatcact ttcgcgaatg aagagttacg gatcccggtg gaagaatgtc ccgttgcgcc
      841 cgagcaactc tttgcagccg aggaagtttt cttctgcggc acgggggtgg aggtggtgcc
      901 gatttcgagc gtgggccggc gtgccattgg cgacggaaaa ccgggtggcg ttacgggccg
      961 cttagcggct ctctacctgc aggtggtgcg cggtgagaaa gaaaaatacc agaagtggct
     1021 ggttgagatt tgaccgctac gaggatgaat aattatgcgc tgggtcacgc gtgaaaaggt
     1081 gaaagtcgac cgggtggcct gcccctggct gatcaaggaa ttcatcgatc cggaagccga
     1141 gttcgtcttc gtcccgcgcg ataccgatcc ggcgagcatt agggatggga ttccgttcga
     1201 catggcgggg gttgaactgg gccaccgcga gggccggtgc agtttcgaat caattctcct
     1261 caagtacggt ctctccgatc cagctctgca cgagatggcg aaaatcatcc acgctgccga
     1321 catcgcggcg gatgaggatt tagtcccgga ggggaaaggg ctgaaggcca ttgccaccgg
     1381 gttcagtctt ttgggattat ccgatcacca aattttagag aaagaattca tcgtctacga
     1441 cgcactctac cgcttttgcc aggaaaaagt gcacaaatga attccgttcc ggaactacgc
     1501 cgaggttagg gtgaaacggc aaaagcgggg ttggtatccc ccgcctttgc attcttaacg
     1561 tggggaagcg gttttgtctt tggaaccgga gtttacccgg ccagtttctt ggcttccgct
     1621 ttcagttttt caaaatcgtc cggcgtgccg ggattgtact tcaggttgat gtaacggatg
     1681 atgccggctt tatcaacgag gaagaccgtc cgcttgttga agcccatttc cggcataaaa
     1741 ctcccgtaag ttttggccac gctgtggtcg tggtcggagg ccaggatgaa ggggaggttg
     1801 tggtgcttgg cccactcctg gtgggagaag acgtagtcgc cggaaatcat gatgacctcg
     1861 cagttcaggt cggtgaaggc ggagaaattg tcgcggtagg agcagacctg cttggtgcag
     1921 ccgccgctcc agtcggcggg gtagaaggcc ac
//
LOCUS       DBNGZA010000201         2708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1417627, whole genome shotgun sequence.
ACCESSION   DBNGZA010000201 DBNGZA010000000
VERSION     DBNGZA010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1417627"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1571)
                     /locus_tag="AC16FU_04180"
     CDS             complement(255..1571)
                     /locus_tag="AC16FU_04180"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR2993603.1"
                     /translation="MPPAKSAEAKSREALTVTDNRTGKTYELPIQDGAIRATDLRQIK
                     VSEDDFGLLAYDPAFMNTACCKSKISFIDGDRGILRYRGYPIEQLAEESNYLETAYLV
                     LYGELPSKAQYDEWVWNITHHTIIHENIKKFIDGFHHDAHPMGMVVSVVAALSTFYPD
                     SKNISSAEVRRKQTYRLIGKMPTLAAFAYRHSKGLFFAYPDNEFSYTGNFLNMMFKMT
                     EVKYKPQPVLEKALDLLFILHADHEQNCSANAMRSVGSSYVDPYCATAAAAAALYGPL
                     HGGANEAVLRMLDEIGSKDKVPEFIRRVKGGEGRLMGFGHRIYKNYDPRAKIIKTMAD
                     QVFEVTGKNPLLDIALELERIALEDEYFVKRKLYPNVDFYSGLIYQAMGFPVDMFPVL
                     FAIPRTSGWIAQWEEMLSDPEQKIARPRQLYLGNVKRDFVPMGKRV"
     gene            complement(1913..>2708)
                     /locus_tag="AC16FU_04185"
     CDS             complement(1913..>2708)
                     /locus_tag="AC16FU_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2993604.1"
                     /translation="RIALKLINPPEARRTFLATFASTEKAAEVVAEITRKGVLPSLVE
                     FVDQDALTITLTYLNKTGFTDAAALLLVEVDGSAGAVEQESGYVLNILQMHALEVRQA
                     VTPEEVEELWLVRREVSTAQRNLFRTKISEDICVPPSRLPEVVAFQKVLVERFGLRVN
                     SFGHAGDGNLHVNFLTNGGVPEERSRIETALEELFDKTLELGGTISGEHGIGVTKSQY
                     LEREVGQAGLELFRKMKAVFDPLGVVNPGKIFFDATVGIGSPSGAY"
BASE COUNT          571 a          778 c          719 g          640 t
ORIGIN      
        1 tctcccaaca gtatttcccg ctctacctga aagagatgga gttccggtac aaccaccgca
       61 aagagcccat gttcgaacga ttggcacaac taatggcaaa tcccgtgtca aatcttttat
      121 aatcaccatg ttttttgttg cgtccgattt tcaaacctga cgcacgtggt aagtcttaag
      181 acctgacaca acccccgaaa tgcaaggcgc gggaaagtta cctcccgcgc cttttcttta
      241 cccggctact tatttcacac gcgcttgccc attggcacga aatcgcgctt cacgttccct
      301 aagtagagct gccgcggccg ggcgatcttc tgttccgggt cggagagcat ctcttcccac
      361 tgggcgatcc acccagaagt gcggggaatg gcaaagagca ccgggaacat atccaccggg
      421 aaacccatcg cctggtaaat cagcccggag tagaaatcga cgttggggta gagtttccgc
      481 ttgacgaaat actcgtcctc cagcgcaatc cgctccaatt ccagggcaat gtccaagagc
      541 ggatttttgc cggtgacctc gaaaacttgg tccgccattg tcttgatgat tttggcgcgc
      601 gggtcgtagt ttttgtaaat ccggtgcccg aaccccatca ggcgcccctc gccccctttc
      661 acccgccgga tgaattccgg aactttgtcc ttggaaccaa tctcgtccag catgcgcaaa
      721 accgcctcat tcgccccgcc gtggagagga ccgtagagcg ccgcagcggc tgctgcggtg
      781 gcgcaatagg gatccacgta cgaactgccg acgctgcgca tggcattggc cgaacagttc
      841 tgctcatggt ccgcgtggag gatgaaaagc aagtccaagg ccttctccag caccggctgc
      901 ggtttatact tcacctccgt catcttgaac atcatgttca agaaattacc tgtgtaggag
      961 aattcattgt ccgggtaggc gaagaagagt cccttcgaat gccggtaggc aaaggcggcc
     1021 agagtcggca ttttgccaat cagccgatag gtctgcttcc gccgcacctc agcggaggag
     1081 atattcttgg agtcagggta gaaagttgaa agagcggcca ccaccgaaac caccatcccc
     1141 atcgggtggg cgtcatggtg gaatccgtca atgaattttt tgatgttctc gtggatgatg
     1201 gtgtggtggg tgatgttcca gacccattcg tcatactggg ctttgctcgg caactcaccg
     1261 tagagaacca ggtaagcggt ttccaggtag ttggactctt ccgccagttg ttcaatcggg
     1321 tacccccggt agcgcaggat gcctctatcc ccgtcgatga agctgatctt gctcttgcag
     1381 caggcggtgt tcatgaaggc cgggtcgtac gccagcagcc caaagtcgtc ctcggaaact
     1441 ttgatctgcc gcaggtcggt ggcccggatg gcaccgtcct gaatcgggag ttcgtaggtt
     1501 ttgccggtcc gattatccgt caccgtgagc gcctcacggg atttcgcttc tgcggatttg
     1561 gccggtggca ttggctcttc agccgtcccg cctctggcgg gggtcggctt tggcggggtg
     1621 gctgcggttg ttttcgtcat cggcgtttgc tcctcttcgt ttatcggaaa aaatctcctc
     1681 ctctactatc ccgcaagaat ctacgtagcg gcggggcaaa atacaagact ttttagtatt
     1741 gaatgccacc cggaaaagtc tttgttcttt tggcgcttac gactttcttt aaccactgac
     1801 agcccggtga gaaaaaccgt ctccctcact ggatggatga agttgctccg gacagagatg
     1861 cccataagta gttatggacg tgataattac cgcgataagc aaaggtgata ctttaatatg
     1921 cgccagacgg agaacctatc cccactgtgg catcaaagaa gattttcccc gggttcacca
     1981 caccgagggg atcaaagacc gctttcattt ttcgaaataa ttccagcccg gcctggccga
     2041 cttctctttc caaatactga gacttggtaa cgccgatccc gtgttcgccg gaaatcgttc
     2101 ctcccagttc cagcgtttta tcaaagagtt cttcgagcgc cgtctcaatc cgggatcgct
     2161 cttcggggac cccaccattc gtgagaaagt tgacgtggag atttccgtca cccgcgtgac
     2221 cgaacgagtt gacccgcagt ccaaaccgtt cgaccaacac tttttggaaa gccaccactt
     2281 ccggcaggcg gctgggcggg acgcagatgt cctcgctgat tttggtccta aaaaggttcc
     2341 gctgggcggt ggagacttcc cgccgcacca accagagttc ttccacttct tccggcgtaa
     2401 ccgcctgtcg gacttcgagt gcgtgcattt gcaggatgtt caatacgtaa cccgattcct
     2461 gttccaccgc gccggccgac ccgtccactt cgaccaacag gagtgcggct gcgtccgtga
     2521 agccggtctt attcaggtag gtgagcgtga tggtaagcgc atcctggtcc acgaattcga
     2581 ccaacgaagg gaggaccccc tttcgcgtga tttcggccac aacctccgcc gccttttcgg
     2641 tggaggcaaa tgtcgccaga aaagtccggc gcgcttccgg cgggttgatt aacttcagcg
     2701 caatccgg
//
LOCUS       DBNGZA010000202         1990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_462906, whole genome shotgun sequence.
ACCESSION   DBNGZA010000202 DBNGZA010000000
VERSION     DBNGZA010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_462906"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC16FU_04190"
     CDS             complement(<1..574)
                     /locus_tag="AC16FU_04190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR2993605.1"
                     /translation="MPRILIDNKYEVVRKLREGGFGVIFYGWDLTLDKPVAIKEIAPS
                     LLGHQQYLDMFTDEALNTAKLSHANIVSVYDLRRTPEGRIFIIMEYLDGVDLRTALER
                     CRTDGVPFPVDLAVQVIAEVCKALEYAHTARDRRTGLPLNIIHRDISPSNIMITATGE
                     VKLIDFGVAKARTRVARKTRTGILKGKVSYM"
     gene            930..>1990
                     /locus_tag="AC16FU_04195"
     CDS             930..>1990
                     /locus_tag="AC16FU_04195"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR2993606.1"
                     /translation="MRYLRLASAITAFLFFALIGAAALAAQSAHPQKVEALRVAEPIK
                     IDGILSESVWQRPATSNFTQRDPTEGVSPTESTHVWVAYDNVAVYVAARLYDRTPDSI
                     TARLGRRDAGMSSDLFAFFVDPYYDRRSGFYFGLNPAGTFYDGVLYNDDWDDDSWDGV
                     WEGKAKIDSLGWTAEMRIPYSQLRFHKKDQYLWGVNFERDIARKNERDFLVFTPKNGS
                     GFVSRFVDLVGIENIEIPYHLEVLPYLTTKAEYTRHAPGDPFNDGSKFLPGTGADLKV
                     GLGSDLTLAATVNPDFGQVEVDPAVVNLSDVETFFQEKRPFFIEGSSIFNFGQGGSNS
                     YWNFNWPGPEFFYTRRIGRA"
BASE COUNT          411 a          599 c          552 g          428 t
ORIGIN      
        1 acatgtagct cactttgcct ttcaggatgc cggtgcgggt cttcctggcg acccgggtgc
       61 gggccttggc cacgccgaag tcgatcaact tcacttcgcc ggtggcggta atcatgatgt
      121 tcgaaggcga aatgtcgcgg tggatgatgt tcaaaggcag gccggtccgg cggtcgcggg
      181 ccgtgtgcgc atactccagc gccttgcaaa cttcggcgat tacctggacc gctaaatcga
      241 ccgggaacgg caccccgtcg gtgcggcagc gctccagggc cgtgcgcagg tcaacgccgt
      301 ctaaatactc catgatgatg aaaatccgcc cctccggcgt gcgccggagg tcgtagaccg
      361 aaacgatatt ggcgtgggac aacttggcgg tgttcagcgc ctcgtcggtg aacatgtcca
      421 agtactgctg gtggcccaga agcgacggcg cgatttcttt gatcgccacc ggcttgtcca
      481 gagtcaaatc ccagccgtag aaaatgaccc cgaacccgcc ctcccgcagt ttgcggacga
      541 cctcgtattt gttgtctatg agaatgcgcg gcattactcg actgagctca cgacgttgtt
      601 atcagttgta gccgaggatt tcatcccata cgggtcgtac cgacctcggc gccgaaggcg
      661 tagatttcac tcaaagcgca aaaaatatct cctccccttg ccggcctttt cctaaactat
      721 caggcgggca acgggttagt cgttggtagt tatatcggca gaaatgtcgt ggcctcgact
      781 ctccaagccg atttgcccgg accggaagag attcccctct tctttttgaa accggaggcc
      841 gcaaaagacg tacaagggac taaagatgca gctaaatgag atcgggtcgg gaaactcgtc
      901 ttgaaactct gagcccttcg caaaaggata tgcgttacct ccgactggcc tccgccatca
      961 ctgcattcct ttttttcgcc ctgattggag ccgccgcgct cgctgcccag tcggcccacc
     1021 cgcaaaaagt tgaagccctg cgtgtcgccg aaccgatcaa aattgacggc atcctttccg
     1081 agtcggtctg gcaaaggccc gcaacgtcca attttaccca gcgagacccc accgagggtg
     1141 tgtcgccaac cgaaagcacg cacgtgtggg tggcgtatga taacgtggcc gtttacgtcg
     1201 ccgcccggct gtacgatcgg acgcccgatt ccatcaccgc ccggctgggc cggcgcgatg
     1261 ccggtatgag ttccgacctc ttcgcatttt tcgtggaccc gtactacgac cggcgttcgg
     1321 gcttttactt tggcttgaat ccggccggaa cgttctacga cggcgtcctc tacaacgacg
     1381 actgggacga cgattcctgg gacggcgtct gggagggtaa agcgaaaatc gactcgctgg
     1441 gctggaccgc cgaaatgcgg atcccttatt cccagcttcg cttccacaag aaagaccaat
     1501 acctctgggg cgttaatttt gaacgggata tcgcgcgaaa aaatgaaaga gattttctgg
     1561 tttttacccc caaaaacggc agcggtttcg tttcccgctt cgttgatctc gtcggtatcg
     1621 agaatattga aatcccctac cacctcgagg tactgcccta cctgaccacc aaagcggagt
     1681 acacccgcca cgctcccggc gacccgttca acgacggctc caaattcctg cccggaacgg
     1741 gcgccgacct gaaggtgggg ctgggcagcg acctgactct ggccgccacg gtcaatcccg
     1801 atttcggaca ggtggaagtc gatcccgccg tggtcaactt gagcgacgtg gagaccttct
     1861 tccaggagaa acgccccttc tttatcgaag gctccagtat tttcaacttc ggtcagggag
     1921 ggtccaatag ctactggaac ttcaactggc cgggccccga atttttttac acccggcgca
     1981 tcgggcgggc
//
LOCUS       DBNGZA010000203         4322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_674462, whole genome shotgun sequence.
ACCESSION   DBNGZA010000203 DBNGZA010000000
VERSION     DBNGZA010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_674462"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..632)
                     /gene="purN"
                     /locus_tag="AC16FU_04200"
     CDS             complement(27..632)
                     /gene="purN"
                     /locus_tag="AC16FU_04200"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR2993607.1"
                     /translation="MKPLPLAVFASGEGTNLQAVINAIESGRLAAKIQFVLSNNSRSG
                     ALARARKHNIPAIHLSEVTCGSREEFVEKLKELLSGNSVELIILAGYMKLVPKEVIFL
                     YRNRIINIHPALLPRFGGPGLYGRRVHEAVLASGVKESGASVHLVDELYDHGPVILQR
                     TVPVKPDDTPETLAARVRGVEHEILVEAIDLFVQGKVPVSS"
     gene            complement(670..2109)
                     /gene="gatB"
                     /locus_tag="AC16FU_04205"
     CDS             complement(670..2109)
                     /gene="gatB"
                     /locus_tag="AC16FU_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR2993608.1"
                     /translation="MTYEPIIGLEVHAQLLTHSKIFCGCSTDFGSPPNSQTCPVCLGL
                     PGVLPVLNRKAVEYAMKMILAVGGQVQPKSIFARKNYFYPDLPKGYQISQFDLPLGKG
                     GAVELEFDDSHKSIGITRIHLEEDAGKSLHPEGNPDRDYSLVDMNRCGVPLIEIVSQP
                     DLRSPREAYLYLLKLKQILEYLEICSGNMEEGSLRCDANVSVRPVGREKFGTKTEVKN
                     MNSFKAVERALTHEIERQTNLLESGREVIQQTLLWDADREEVEPMRQKEESDDYRYFP
                     EPDLLTLTVDADWVNTVKATLPELPDQKKNRFVTQYELSTYDAEVLTGEKAIANYYED
                     YVALYPKPKVVANWVMGEVLRELKERKITIQEFAVPPRQLASLLEKIDAGVLSAKMAK
                     EVFTEMAATGDAPEAIIQKRGLVQVSDTAELEKVVEAVLAENPENLKRYREGKDKLFG
                     FFVGEVMKKTKGQANPQVVNTILKSKLAQ"
     gene            complement(2140..2586)
                     /locus_tag="AC16FU_04210"
     CDS             complement(2140..2586)
                     /locus_tag="AC16FU_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993609.1"
                     /translation="MEFLKVANMRTSIKPSLITVLAILVFLGVLAFGVVKWYNGQEKK
                     YSQVMVRILRSVSLPDTATFKDYAKVLRREIKPGFTVREVHAVMRGYDRIETFPFWET
                     KFPSWKYFLEYYRYDFPWIDEFFPGPTAIIIEYDSSWNFKSFATQP"
     gene            complement(2564..4009)
                     /gene="gatA"
                     /locus_tag="AC16FU_04215"
     CDS             complement(2564..4009)
                     /gene="gatA"
                     /locus_tag="AC16FU_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR2993610.1"
                     /translation="MELNRLKAWELLPRLEKKEISATELVTQTLAEIGRTGKTLNTFI
                     TVTAEQALSQAKQIDARRGRGEKVGALAGIPIAIKDVICTQGIQTTCGSRILTGFVPP
                     YDATVITKLKVADAVIVGKTNMDEFAMGSSNENSAFGPVRNPHDPSRIPGGSSGGSAA
                     AVAAHQAIFALGSETGGSVRQPASLCGVVGLKPTYGAVSRWGLVAFASSLDQIGSFGR
                     DVRDCALLFDIIAGGDPRDSTSRKAYQPHCFEKLRQEPKSFRIGVPKEYFALGIEPDV
                     ERLVRGAIDRIGREGVPVEEVSLPSTDKGIATYYIIAPAEASSNLARYDGVKYGLRVY
                     GGNDDLLTMYGKTRQEGFRPEVKRRIMLGTYVLSAGYYEAYYLKAQKLRAQVKKDFDD
                     AFRKVDLLVCPTSPTAAFKLGEKVDDPLTMYLNDVYTVTANLAGIPAISIPCGKTKAG
                     LPVGLQILAPPFAEEKLFQFAHWVDGILKSS"
     gene            complement(4068..>4322)
                     /locus_tag="AC16FU_04220"
     CDS             complement(4068..>4322)
                     /locus_tag="AC16FU_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR2993611.1"
                     /translation="VAFLLYQKTLIDCGDGVLLRARDWDAVTRKVTAFFKENGTMTVG
                     QLRDLLHTSRKYAVPILEKLDHVGLTRRVGDQRVLVDTHD"
BASE COUNT          920 a         1194 c         1204 g         1004 t
ORIGIN      
        1 aaaggtggaa tgtgtttcaa cgacgctcag gacgaaacgg ggactttacc ctggacgaag
       61 aggtcgatcg cttcgaccag aatctcatgt tccactcccc ggacgcgggc cgccagcgtt
      121 tcgggagtat cgtcgggttt taccggcact gtgcgctgga ggatgaccgg tccgtgatcg
      181 tagagttcat cgaccaagtg taccgacgcg ccgctctctt tgaccccgga agccagaacg
      241 gcttcgtgga cgcgcctgcc gtacaggccg gggccgccga agcgcggcaa gagcgccggg
      301 tggatgttga tgatccgatt gcggtagaga aaaattacct ctttcggcac cagtttcata
      361 tagccggcca gaataatcag ttccacacta ttgccggaga gcagttcctt gagcttttcc
      421 acgaattctt cacgcgaccc acaggtcact tccgaaaggt gtatcgccgg gatgttgtgc
      481 ttgcgggcgc gcgccagcgc gccggatcga ctgttgttgg aaaggacgaa ttgaattttg
      541 gcggccagcc gtcccgattc gatggcgttg atgacggcct ggagattggt gccctcgccg
      601 gaagcgaaaa cggccagagg aagcggtttc atctccggga aaagaatcct accgtaaggg
      661 gcaagaggtt cattgcgcca gtttgctttt gagaatggta ttcacgacct gcggattggc
      721 ctggcccttc gttttcttca tcacttcccc gacaaagaag ccgaagagtt tatcttttcc
      781 ctcgcggtag cgtttgagat tttccggatt ttcagccagg accgcctcca ccactttttc
      841 cagttcggcg gtatcggaaa cctgcaccaa tccgcgtttc tggatgattg cctccggcgc
      901 atcaccggtg gccgccattt cagtgaacac ttccttggcc attttcgcgc tcaacacacc
      961 ggcgtcaatt ttttctaaga gcgacgcaag ctgccggggc gggacggcaa actcttggat
     1021 ggtaatctta cgctccttga gttcccgcag gacctccccc atcacccagt tggcgacgac
     1081 cttcggcttg gggtaaagcg ccacgtagtc ttcatagtag ttggctatcg ctttttcgcc
     1141 tgtgagcact tccgcatcgt acgtcgagag ctcgtactgc gtcacgaagc ggtttttctt
     1201 ctgatcgggc agttccggca gggtggcttt gaccgtattg acccagtccg catcgaccgt
     1261 cagggtcaga agatcaggtt ccgggaagta gcggtagtcg tccgattcct ctttctggcg
     1321 catcggctcc acctcttccc ggtcggcgtc ccagaggagt gtctgctgaa tcacctcccg
     1381 tcccgattcg aggagattcg tttgacgctc gatttcgtgc gtcagcgccc gctccaccgc
     1441 cttgaacgaa ttcatattct tgacttcagt cttggtgccg aatttttccc ggccaaccgg
     1501 ccggaccgag acgttggcgt cgcagcgcag actgccctct tccatattgc cggagcagat
     1561 ctccagatac tccaggattt gctttaactt caagagataa agatacgcct cgcgggggct
     1621 gcgcaggtcg ggctgggaga cgatttcgat caaaggcacg ccgcagcggt tcatatcgac
     1681 gagagagtag tcgcggtcag ggtttccctc cgggtgaagc gatttgccgg cgtcctcctc
     1741 caggtgaatg cgggttatgc ctatgctctt gtgtgagtca tcaaattcaa gttcgaccgc
     1801 ccctcctttg ccgaggggta aatcaaactg cgagatttga taccctttgg ggaggtcggg
     1861 gtaaaagtag ttcttccggg caaagatgct tttgggctgc acctggcccc ctaccgccaa
     1921 gatcatcttc atagcgtact ccaccgcctt gcggttcagg acgggcaaaa caccgggcag
     1981 gccgaggcag accggacagg tctgggaatt gggcggactg ccgaagtcgg tcgagcagcc
     2041 gcagaagatt ttggagtgcg tcaggagttg ggcgtggact tctaaaccga tgattggttc
     2101 gtaggtcatt atatttcggt cggttacgga cgactttacc taaggctgag ttgcaaaact
     2161 cttgaagttc cagctggaat catactcaat tatgattgcc gttggacctg gaaaaaactc
     2221 gtcgatccaa ggaaaatcat atcgataata ttctaaaaaa tatttccaag aaggaaactt
     2281 agtttcccaa aaagggaacg tttcaattct atcgtaaccc ctcataacgg catggacttc
     2341 acgaacagta aaacccggtt ttatttctcg ccgtaacact tttgcatagt ccttaaaagt
     2401 tgcggtatcg ggaagtgaca cgcttcttaa aatacgtacc ataacttgac tgtatttttt
     2461 ctcctgaccg ttataccact tcaccacccc gaatgccagc actcccaaaa agaccagaat
     2521 tgccaaaacg gtaattaaag atggttttat tgacgttctc atattagcta cttttaagaa
     2581 ttccatccac ccagtgtgca aactgaaaca acttctcctc agcaaaaggc ggagccagaa
     2641 tttgcaaccc taccggcaag cccgctttgg ttttcccgca agggatcgaa atcgccggta
     2701 tgccggctaa gttggccgtg acggtataaa cgtcgttcag gtacatcgtc aaaggatcat
     2761 cgactttttc gcccagtttg aacgccgcgg tgggggaagt cgggcagacc aacaggtcaa
     2821 cttttctgaa ggcgtcatcg aaatcttttt tcacctgcgc ccggagtttc tgggctttga
     2881 ggtagtaggc ctcatagtag ccggcggaaa gtacgtaggt gccgagcata atgcggcgct
     2941 tgacctccgg cctgaacccc tcctgccggg tcttgccgta catcgttagc aggtcgtcat
     3001 tgccgccgta gacccgcagg ccgtatttga ccccatcgta tcgcgccagg ttggacgacg
     3061 cctcagccgg agcgataatg tagtaggtgg cgatcccctt gtcagtggag ggcagggaga
     3121 cttcttcgac cggcacgcct tcccggccga tgcggtcgat ggcgccccgc accaatcgct
     3181 ccacgtccgg ctcaatcccc agggcaaaat attccttcgg gacgccgata cggaaacttt
     3241 tcggctcctg cctcaacttt tcgaagcagt gcggctggta tgccttccgc gaggttgaat
     3301 cgcgcgggtc gccgccggcg atgatatcga agagaagcgc acagtcgcgc acgtccctgc
     3361 cgaaggagcc gatttgatct aaagaggaag cgaaagccac caatccccag cgagagacgg
     3421 cgccgtaggt cggcttgagt cctaccacgc cgcagagaga ggccggttgg cgcaccgacc
     3481 caccggtttc ggagccgagg gcgaaaatcg cttgatgcgc ggccaccgct gcggctgacc
     3541 cacccgagga accgcccgga atgcggctgg ggtcgtgcgg gttgcgcacc gggccgaaag
     3601 cggagttttc gttcgacgaa cccatggcga attcatccat attggtttta ccgacgatga
     3661 ccgcgtcggc aaccttgagt ttggtgatca ccgtcgcatc atagggcggg acgaatccgg
     3721 tcaaaatccg cgaaccgcag gtcgtctgga tgccttgagt gcagatgaca tctttaatcg
     3781 ctatcggaat tccagccaga gcaccaacct tttcgcctcg accgcgccgg gcatcgatct
     3841 gttttgcctg ggacagcgcc tgctcagcgg tgacggtgat gaaggtgttg agcgttttgc
     3901 ccgtgcgccc gatttccgcc agcgtctgcg tgaccagttc ggtggcggaa atttcttttt
     3961 tctccagacg agggaggagt tcccaggctt tgagccggtt gagttccaca cggagaatat
     4021 agcagatgtt agttgatgga aaaaatattt gcggtagaac gaaaagctca gtcgtgggtg
     4081 tcgaccagaa cgcgctgatc ccccacccgc cgggtgagac ctacgtggtc taatttttcc
     4141 agaatcggca cagcgtattt gcgcgaggta tgcaggaggt cgcgaagctg gccgaccgtc
     4201 atcgtgccat tttctttgaa aaatgccgtc acttttcggg tgactgcgtc ccagtcacgg
     4261 gcgcgtagca acacgccgtc gccgcagtcg attaaggtct tctggtaaag caggaaagcc
     4321 ac
//
LOCUS       DBNGZA010000204         7455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1840853, whole genome shotgun sequence.
ACCESSION   DBNGZA010000204 DBNGZA010000000
VERSION     DBNGZA010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1840853"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1350)
                     /locus_tag="AC16FU_04225"
     CDS             complement(172..1350)
                     /locus_tag="AC16FU_04225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993612.1"
                     /translation="MGKRSRRIGIDVGPLTWPVQSGIPTFVRQLVSGLNSTPEAAAAG
                     AGAISFGYYRFSHRGWSPLAREFVTSGLRIAGLPLPMRLARWLWDWPGFLDVAGVKQY
                     DLVVGTVSELPPLTPQQKGLSVFHDLAGFKIPGAFSAPAVRQRRAIYQKTLERSHRIL
                     VYSRSTWRDLVELFGVPEEKLRLVPLGIELAPFQKKIGPEEKNRILRKYGISPPFIFF
                     SGIIQPRKNLARLAEAFLSLKEEEKLPHQLVLAGGLGYQGKEILEVIRSQDKSGAIHY
                     TGYVAASDLPLLYQAADLFVYPSLYEGFGLPVVEAMASGTPVVASYSSSIPEVVGQAG
                     VLVDPESPEAIASGMIQVLGNRSLASRLSLQGSRRARQFSHRAMARKFMAVCEELLRA
                     "
     gene            complement(1337..1870)
                     /locus_tag="AC16FU_04230"
     CDS             complement(1337..1870)
                     /locus_tag="AC16FU_04230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2993613.1"
                     /translation="MVVVDFGCGRRKHPGTIGLDRNPRSDADIICDLNHPVPLPDSSV
                     DGVICKDVLEHVGDLVFTMEEIHRICKPGAKVEIQGPFASSPEFFHDPTHRRAFTSQS
                     FDYFIEGTPAAQFRYSDAYFRLEKVYYSRGRVRLLDRLFLSLANRFKRQFEARFLFWY
                     PVNTIHFVLRTVKNGKA"
     gene            complement(1861..3369)
                     /locus_tag="AC16FU_04235"
     CDS             complement(1861..3369)
                     /locus_tag="AC16FU_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR2993614.1"
                     /translation="MKSAAPAASPAAQAARPLGTQVFWNSGALLFGSVANKLLRLGAA
                     LFLARYLGATGFGRLALVFAFAEIFRLICDLGLDVVAVRQLARVPSQADRLIPATILL
                     KAGLSVLAILAAGGTALACGYSETLVLLISLATLASAFQSLASGFGALFRARLEVSRF
                     LPVLLAEGAVFFALVVCGTLTRQGLPFFVGVFSVAACLAAVLAFRLARRITSFNWNFD
                     RSLVWDLLRQAWPLTVSLIFGAIYFRIDTILVNALAGDRATGLYSACFKLTETFLLVP
                     SAVAASLLPVLSLLRRENPARMIAAFRKTYQLLLALGIFASGLLAFAAAPLVKAAYGA
                     EFGEAAGGLAVLAWSLTFMFVNMLWTQALLAYDCQKKLAWVTLGNVLVNVGANLVLIP
                     IAGFRGACIATVLTEAVSFVLQGVIIRRLAGSLGILKATAQGAALAAGAVALETAARS
                     LSSGWLVTGIAVWSGLMLAFLFSLRGVLGRLPLFPFFRSPAQETVFPEVPWS"
     gene            complement(3366..4490)
                     /locus_tag="AC16FU_04240"
     CDS             complement(3366..4490)
                     /locus_tag="AC16FU_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HIR2993615.1"
                     /translation="MIPDQFEPEAKNKLTLVRPNPAGGGEGVRWTDLVAAVVARRKLI
                     YAIVGTTTILAVTVSLLLSNRYTARAVILPSGERTNLQKLAGLAGGSLLGLGIDPSAV
                     GIENSSLLFPDVLKSRTVREGVLAATFRFPRGKRTVETTLLEYFGVRSLDRGVKRLKN
                     VTDITMNPKTGVIALEVTLEHPELSAAVANEFIRQLDRYNQETRLSKAKDNEAFIARR
                     LEEVGGELRTAEDDLEKFQAQNRNYYAALAPDLTKELKRLQREVEVKNQVFLTLTQQH
                     ELAKIEAKRDLPIVQVLDYARPPEVKSFPQRSLIVLAAFMLSLAFSIFWALWSAYLGH
                     QLSLEERDRLRQQLQAFSADRRRLWQFVRRPLPKFEVPAS"
     gene            complement(4511..6904)
                     /locus_tag="AC16FU_04245"
     CDS             complement(4511..6904)
                     /locus_tag="AC16FU_04245"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR2993616.1"
                     /translation="MQSQTLAWLATAGLLFFSFAAITSTFAQDLTPEEKARLIVEYEK
                     LKQTTGGAPPDAAKLLETQTYQTPDIYSSIPEPKPDAVKSNAGDKGSIFPEGKMPVPK
                     PAATSSEELKLFGYDIFTAQPSSFAPVEDIAPPVDYTLGPGDNLIVNLWGRVDLELNL
                     TCDREGKVFIPKAGEVIAWGLTVPEFEKRLRSKLSTVYSNFRLTVILGKLRTIKVYVF
                     GEVKRPGGYAVTSLASLFNALYLAGGPNERGSLRQIRLVRQNQLVATVDFYEFLLKGF
                     NRDDLKLLSGDVIYVPVVGPLVKIRGQVKRPAIYELKDAERLTAVIALAGGTLPNAYL
                     ERVMIDRIDAGDRRQLIDLNLRDSTVLAENNLTIHDGDDISVFSKYELRENMVWLTGQ
                     VKHPGTFERTAGMTIRDLLKGGEFLEYEAYRHRADLYRLTHNRVRAIIPVNLDLALAG
                     DSTMNFVLQDRDSLRIYSRQEVEHPKFVFISGEVRQPGKYDLLDSMRLWDLIFLSGNL
                     DKSAYLLRAEVARMNPGKPADVLFVNLADVQADPESPQNLFLKEDDQVFIREIPQYRM
                     QENVKITGEVQFPGEYTLQKENETLYALLKRAGFFTERAFAKGTIITRQEVVAEIRRQ
                     NLPDIVANTQRLVKNDSTGQIERPNLDAFDPAKATRIVVDLEEILRTEGKKGDIVLRR
                     GDQIHVPDLPAGIPVMGAVAANGTINFARGKKLGYYVERAGGFTPNADKKEVRLVKAN
                     GRVYSGGKARGQKVELGDAVVVTTRVQEKKDWLKIFASTASILSGVATTAFVIDRLR"
     gene            7141..>7455
                     /locus_tag="AC16FU_04250"
     CDS             7141..>7455
                     /locus_tag="AC16FU_04250"
                     /inference="COORDINATES: protein motif:HMM:NF042609.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HIR2993617.1"
                     /translation="MEKLFPLTYEFFSSSPVAEDYILGPPFSNCSAGWSTYPALFSFH
                     IPTYLTPVQNWSHPGGKLRELGAESLSDAELLSIIISPGIKGKSAEKIAEDILAKFQS
                     FKG"
BASE COUNT         1597 a         2236 c         2235 g         1387 t
ORIGIN      
        1 agaccgacgg cgaccacaag tgccaggatc ggaagtgccg ccaccggccc ggctccggcc
       61 actatccaga cgctcagagc cgccgcgccg aagaccagaa ggaaggcgcg ggaggaaagg
      121 aaactccaac ccgcgagcgc tcgcgtctct gcggccaggg tggctgcaaa ctcacgcacg
      181 gagcagttcc tcgcagaccg ccataaattt ccgggccatt gcccggtggg aaaactgtcg
      241 ggcgcgccgc gagccctgaa gggagagccg ggaagccagg ctgcggttgc ccaaaacctg
      301 aatcataccg ctggcaatcg cttccgggct ttccgggtca accaagacgc cggcctggcc
      361 gaccacttcc ggtatggatg aactatagga cgccaccacc ggtgtgccgg aggccatcgc
      421 ttccacgacg ggcaggccga acccttcata cagggaggga taaacgaaaa ggtctgcggc
      481 ctggtaaagg agggggaggt cactggcggc aacgtaaccg gtgtaatgga tggcgccgct
      541 tttatcctga gacctgatca cctcaagaat ttcttttccc tggtaaccca gaccgccagc
      601 gagcaccaac tggtgtggca gcttttcttc ttctttgagc gagaggaaag cttcggccag
      661 gcgggccaaa ttcttgcgcg gttgaataat gccgctgaaa aaaatgaagg gtggggaaat
      721 tccatacttt ctcaagatcc tatttttctc ttccgggccg atttttttct gaaagggagc
      781 cagctcgatt cccaggggga ccaagcgcaa tttctcttcg gggaccccaa acagttccac
      841 caagtcccgc cacgttgagc gggagtagac cagaatgcgg tgggagcgtt ctaaggtctt
      901 ctggtagatg gcccggcgct ggcgcacagc cggtgcggaa aaagcacccg gaattttgaa
      961 gccggccagg tcgtggaaga cgctcaaacc tttctgctga ggcgtcaaag gcggcagttc
     1021 ggacacggtg ccgacgacca agtcgtactg tttcactccg gccacatcca gaaatccggg
     1081 ccaatcccag agccagcgcg ccagacgcat gggcaaaggc agccccgcaa tcctcagccc
     1141 gctggtgaca aattctcgcg ccaaaggcga ccacccccgg tgggagaaac gatagtaacc
     1201 gaaggaaatg gctccggccc cggccgcggc tgcttccgga gttgagttga gcccggatac
     1261 gagttgccgc acgaaagtcg ggattcccga ctgcaccggc caggtgaggg gtcccacgtc
     1321 tatgccgatc ctccggctac gctttcccat ttttcaccgt ccgcaggacg aagtggatgg
     1381 tgttcaccgg gtaccaaaag aggaaccgcg cttcgaactg ccgcttgaag cggttcgcca
     1441 gagaaaggaa caggcggtcc aacaaccgca cgcggccgcg cgagtagtac actttttcca
     1501 gccggaaata ggcgtcggag tagcggaact gggccgccgg ggtgccctcg atgaagtagt
     1561 cgaacgactg cgaagtaaag gcccggcggt gggtcgggtc gtgaaagaat tcggggctgg
     1621 aggcgaaggg tccctggatt tccactttcg cacccggctt gcagatccgg tgaatttcct
     1681 ccatggtgaa aaccagatcg ccgacgtgct ccagcacgtc tttgcagatc accccgtcca
     1741 ccgagctgtc gggcagggga accggatggt tcaaatcgca gatgatgtcg gcgtccgacc
     1801 ggggattgcg atcgaggccg atcgtccccg ggtgcttgcg ccgcccgcac ccgaaatcga
     1861 ctacgaccat ggcacctccg ggaagaccgt ttcttgggcc gggctgcgga aaaacgggaa
     1921 gagcggcaga cgccccagca cgccccgcaa ggaaaagagg aacgccagca tgagcccgct
     1981 ccagacggca atgcccgtta cgagccagcc gctggagagc gaacgtgcgg ccgtctccag
     2041 cgccacggcc ccggcggcca gggcggcacc ctgcgcggtc gcctttaata tgcccagaga
     2101 accggccagt ctgcggatga taaccccctg caaaacaaaa ctgaccgctt cggtcaaaac
     2161 ggtcgcgatg caggcaccgc gaaagccggc gatcggtatg agcaccaggt tggcgccgac
     2221 gtttacgagc acattcccca aagtcaccca ggccaacttt ttctggcagt catacgctaa
     2281 gagcgcctgg gtccagagca tgttgacgaa catgaaagtc aaagaccagg ccaggactgc
     2341 caggccgccc gccgcttcgc cgaactcggc gccgtaggcg gctttgacga gcggtgcggc
     2401 agcaaacgcg agcaggccgg aagcgaaaat tcccagcgcc agaaggagct ggtaggtctt
     2461 gcggaaagcc gcgatcatcc gcgccggatt ttcccggcgc aggagggaga gaaccggcaa
     2521 aagcgacgcc gccactgcgg aggggaccag taaaaaggtc tcggtcagct tgaagcaggc
     2581 cgagtaaagt ccggtggcac ggtcaccggc caacgcattg accaggatcg tgtcgatgcg
     2641 gaagtagatg gcgccgaaaa tgagtgaaac cgtcagcggc caggcctgcc tgagcaagtc
     2701 ccaaacgaga gagcggtcaa aattccagtt gaaggaagta atgcgccggg ccaaccggaa
     2761 agccaagact gccgcaagac acgcggcgac gctgaagaca ccgacgaaaa aaggcaaccc
     2821 ctgccgcgtg agtgttccgc agacgaccag ggcaaaaaaa accgctcctt cggccaggag
     2881 aaccggcaga aagcggctca cttctagccg cgcccgaaag agcgcaccga aaccggaagc
     2941 cagcgattga aacgcggagg ccagggtcgc gaggctgatc aaaagcacga gcgtctccga
     3001 gtagccgcag gccagtgcgg tgccgccggc cgccagaatg gccagaaccg agaggccggc
     3061 tttgagcagg atcgtggccg gaatcagccg gtcggcctgg gacggcaccc gcgctaactg
     3121 ccgcaccgcc accacgtcca gacccagatc gcagatcaag cgaaagattt cggcaaaagc
     3181 gaagaccagc gccagccggc caaatccggt cgcaccgagg tagcgcgcca aaaagagcgc
     3241 tgcccccagg cggagcagct tgttggcgac actgccgaaa aggagcgcgc cggagttcca
     3301 gaaaacctgg gtgccgagcg ggcgggcggc ctgcgcggcg ggggaggccg ctggcgcagc
     3361 acttttcatg aagcgggcac ctcgaatttc gggagaggac gccgcacgaa ttgccagagg
     3421 cggcggcgat cggccgagaa cgcctgtaac tgctggcgca accggtcgcg ctcttcaagg
     3481 gagagctggt gcccgaggta ggccgaccag agcgcccaga aaatcgaaaa ggccagagac
     3541 agcataaatg cggccagcac aatgagcgag cgctggggaa aactcttcac ctcgggcggg
     3601 cgggcgtagt ccagaacctg gacaatgggt aagtcgcgct tggcttcgat tttggccagt
     3661 tcatgctgct gggtgagagt tagaaagacc tgatttttca cttctacttc gcgctggaga
     3721 cgctttaatt ctttggtcag gtcgggtgct aaggccgcat agtagttccg gttctgcgcc
     3781 tgaaattttt ccaaatcgtc ttcggcggtc ctcaattcac cccccacttc ctccagccgc
     3841 cgggcgatga aggcttcgtt atctttggct ttggagagtc gggtctcctg gttgtagcga
     3901 tccagctggc ggatgaactc gttggccacc gcggcggaaa gctccgggtg ttcgagcgtc
     3961 acctccagcg caatcacccc ggtcttggga ttcatggtaa tgtcggtgac atttttcagc
     4021 cgtttcaccc cgcggtcgag cgaccgaacc ccgaaatact caaggagcgt cgtttccacc
     4081 gtacgctttc cccgagggaa acggaaggtg gctgccagca ccccttcccg caccgtgcga
     4141 cttttcagaa cgtcggggaa aaggagagag gagttttcaa tgccgactgc ggaggggtcg
     4201 atccccaggc ccaaaagact ccccccggcg agcccggcca atttctgcag gttggtccgc
     4261 tcgcccgaag gcaaaatcac cgcccgggcg gtatagcgat tggaaagcaa gagcgacacg
     4321 gtgacggcca gaatggtcgt cgtcccgacg atggcgtaga tcagtttccg acgggccacc
     4381 acggcggcga ccagatcggt ccagcgcacc ccctccccgc cgccggcggg gttcgggcgc
     4441 actaaagtca atttgttttt ggcttccggc tcgaactggt cgggaatcat aggcacttgc
     4501 gcaaggtcgt tcagcggagc cggtcgatca cgaacgccgt agtggcgacg ccggagagaa
     4561 tcgacgccgt gctggcgaaa atcttcagcc agtctttttt ctcctgtact ctcgtcgtga
     4621 cgacgacggc atctcccaat tccactttct ggccgcgcgc tttcccgccg ctgtagaccc
     4681 ggccgttcgc cttcaccagg cgcacttctt tcttgtcggc attgggggtg aagccgccgg
     4741 cgcgctccac gtagtagccg agcttcttcc cgcgcgcgaa gttaatggtg ccgttggcgg
     4801 ccaccgcgcc catcaccgga atgccggccg gcaggtcggg gacgtgaatc tggtctcccc
     4861 gtctgagtac gatgtccccc tttttccctt cagtgcggag aatctcttcg agatccacca
     4921 cgatgcgggt cgccttggcg gggtcaaaag catccaagtt cggtcgctcg atctggccgg
     4981 tggaatcgtt ctttaccagc cgctgggtat tcgccacaat gtcgggcagg ttctggcggc
     5041 ggatttcagc cacaacctct tgacgggtga tgatcgtgcc cttcgcaaaa gcccgctcgg
     5101 tgaaaaatcc ggcccgtttg aggagcgcgt agagcgtctc gttttccttc tggagggtgt
     5161 attcgcccgg gaactgcacc tccccggtaa ttttgacgtt ctcctgcatc cggtactggg
     5221 gaatttcacg gatgaagacc tggtcgtctt ccttcaggaa caggttctgc ggcgattccg
     5281 gatcggcctg cacgtcggcc aggttgacga agaggacatc cgcgggctta ccggggttca
     5341 tccgggccac ttcagcccgc aaaaggtagg cgcttttgtc gagattcccc gagaggaaga
     5401 tcaaatccca caggcgcatg ctgtccaaaa ggtcgtactt tccgggctgg cgcacttcgc
     5461 cggagatgaa aacgaacttg ggatgttcca cttcctgccg cgagtaaatc cgcagtgagt
     5521 cacggtcctg caagacgaag ttcatcgtgc tgtcgcccgc taaagccaga tccagattca
     5581 ccggaatgat ggcgcgcacc cggttgtgcg tcaggcggta gaggtcggcc cggtgccggt
     5641 aggcctcgta ttccagaaac tcgccgcctt tgagcagatc gcggatcgtc attcccgcgg
     5701 tgcgttcaaa ggtgcccgga tgcttcacct ggccggtgag ccagaccata ttttcgcgca
     5761 gttcgtactt ggagaagacc gaaatgtcgt cgccgtcgtg aatcgtaagg ttgttttccg
     5821 ccagcacggt gctgtctcgc aggttgaggt cgatcaattg ccgccggtcg ccggcatcga
     5881 tccggtcgat catcacccgt tccaggtagg cgttggggag cgtccccccg gccagagcga
     5941 tgaccgcagt caagcgctcg gcgtctttca gttcatagat cgccggacgc ttgacctggc
     6001 cccggatttt caccaagggc cccaccaccg gcacgtaaat cacgtcaccg gagagaagtt
     6061 tcaggtcgtc gcgattgaag cccttcagga gaaactcgta gaaatcgacc gtggccacca
     6121 actggttttg ccggaccaat cggatctgcc tgagactccc ccgctcgttc ggcccgccgg
     6181 ccagatagag cgcattgaaa agggaagcca gggaggtgac cgcatacccg ccgggccttt
     6241 tcacttcgcc gaagacgtag actttgatcg tccgcagttt gcccaggatg acggtcaacc
     6301 ggaaattgga ataaaccgtg gagagtttcg accgcaggcg cttttcgaat tccggaaccg
     6361 taagccccca cgcgatgacc tcgcccgctt tgggaatgaa gactttccct tcgcggtcgc
     6421 aggtgaggtt cagttccaga tcgacccggc cccagaggtt gacgatgaga ttatcgccgg
     6481 gacccagagt gtaatcgacc ggcggggcga tatcctccac cggtgcaaag gacgacggct
     6541 gagccgtgaa gatatcgtaa ccgaacagtt tcaattcttc actgctggtt gccgcaggtt
     6601 tcgggacggg cattttccct tccggaaaaa tcgaaccctt gtcccccgca ttggacttta
     6661 ccgcatccgg tttcggctcg ggaatggacg agtaaatatc cggagtttgg taggtttgcg
     6721 tttccaggag tttggccgcg tcgggcggcg ccccgccggt ggtttgcttc aacttttcat
     6781 attcgacgat gagccgcgct ttctcttccg gcgtcaggtc ctgagcaaag gtggaagtga
     6841 ttgcggcgaa cgaaaagaaa agcagtcccg ccgttgccaa ccacgcaagc gtttgggact
     6901 gcacccgccc cctattccgc cctggccgtt cagagcgtat gaccgtacgg gcaggccata
     6961 ggcggaaaga cttaaagccg tgattcctat tcacgtaaac ctccttcttc ggcggtctta
     7021 attggcaaac cggatgccgc ccgtacgggc ccgaagcccc tttttgaaaa accttaacta
     7081 attgcctgtt aatgagatag cagactggct atcggtcgaa accaataccg gacgggacgg
     7141 atggaaaaac ttttcccctt gacctacgaa tttttttcca gtagccccgt agccgaggat
     7201 tacattctcg gtcccccctt ctcaaattgt agtgcgggat ggtctaccta tcccgcactt
     7261 ttttcatttc acattccaac atatcttact cccgtgcaga actggtcaca cccgggcggc
     7321 aaactgcgcg aacttggcgc cgaaagtctc tccgatgccg aactcctctc catcatcatt
     7381 tcaccgggaa ttaaaggcaa atccgccgaa aaaatcgccg aggatatcct tgccaaattt
     7441 caatctttca aaggt
//
LOCUS       DBNGZA010000205         3328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1950724, whole genome shotgun sequence.
ACCESSION   DBNGZA010000205 DBNGZA010000000
VERSION     DBNGZA010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1950724"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16FU_04255"
     CDS             complement(<1..236)
                     /locus_tag="AC16FU_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993618.1"
                     /translation="MRKILCVLALCSIALTVAAAQQQEKSKSGQPEGQSMGMMMPKPG
                     PELAKLDYFLGTWTGEETLNYPGMPAPAKSKAKYT"
     gene            complement(265..867)
                     /locus_tag="AC16FU_04260"
     CDS             complement(265..867)
                     /locus_tag="AC16FU_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR2993619.1"
                     /translation="MRKSLLVLAVGLFAVTSIVAQQEQSKSNKPAEPSMEMMMPKPGP
                     ELAKLDYFLGTWTGEETMNSPGMPPGSKNKSKTVTKRGIGGMFLVGDYTSSMGEGKNA
                     MKFAGHSLWTYDPQKKGYRHWWFDSFGDGQETPGNWVDDKTYVGETSYEMMGQQVKQR
                     QTYAILSPTQFTMKIEMDNGQGWMTFLEGTYTKQTQKPGK"
     gene            complement(962..2890)
                     /locus_tag="AC16FU_04265"
     CDS             complement(962..2890)
                     /locus_tag="AC16FU_04265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2993620.1"
                     /translation="MKKIRKLWFAPLSILLFMAGLSTANAYEFTQYLNIKHANSPSFS
                     PDGKTLAFMTNVTGVYQGWTIPVTGGWPEQATFYEDGVDFFVYSPMEDKIAFGKDTGG
                     DERTQLYLCSPRGENIVQLTNNPKVIYSFGGWSHDGKKIAYAANERDQAYFDVYVRDL
                     AIDQAKLVYQSDANNSVAAWSPDDRYLVVARANTNLDFDLFLVEAATGTSTHLTPHTP
                     PAVYESIEWTNDGKGFYLASDQDRDFVNLAYYDVAEKKLTFLDDGKSDVDGLKLSDDN
                     RYLAIVRNIDGYGRLFLREHPLRVGTITQAKTSEVHLPDGIPSGLTFSKDGKMMAFAF
                     VGPTQNGNIWTYEMAGEKLTEVTRSTLAGIPPSSFVAPTLVKFKSFDGLELSAFIYLP
                     TGAKKDGSAPCIVMVHGGPESQERPYFATTYQYYLSRGYAILAPNIRGSTGYGKTYTH
                     LDDIEKRGQAIQDVKACYNFLKNSGYVDPAKVAVMGASYGGYMTLAAVTFLPELWAAG
                     VDIVGIANFETFLKNTGAWRRKLREAEYGYLDKDIEVLRKYSPIHAVDKIKCPLFVIQ
                     GANDPRVPQSEAEQIVDQVKKQGGAVEYMLFPDEGHGLAKLANRIKAYPAVAEFLDKH
                     VKNRGVVQAGDSSGEKEK"
     gene            complement(2928..>3328)
                     /locus_tag="AC16FU_04270"
     CDS             complement(2928..>3328)
                     /locus_tag="AC16FU_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993621.1"
                     /translation="KGITLVVTEGFGQINMAERTFTLLKQNEGKLACFNGATQIRAGV
                     IRPEVVIPLVGDIRQDEAAEKFAEEGLAEGSPVRVIRQPYFGHLGKVTTLPPELQKLE
                     SESHARILQVEFADGTRAIVPRANVEMLEG"
BASE COUNT          614 a         1032 c          914 g          768 t
ORIGIN      
        1 gtgtacttcg ccttgctctt ggccggtgcc ggcatgcccg ggtaattgag cgtctcctcg
       61 cccgtccagg tgcccaagaa gtagtcgagc ttggccaatt ccgggcccgg tttgggcatc
      121 atcatcccca tcgactgccc ttccggctgc ccggatttgc ttttctcctg ctgctgcgcc
      181 gccgcgacgg tcagcgcaat cgaacagagt gcgagcacgc aaagaatttt ccgcattcct
      241 cgtcctttcc acccgtgtga atgattactt accgggtttc tgggtctgct tggtgtacgt
      301 cccttccagg aaggtcatcc atccctgacc gttgtccatc tcgatcttca tggtgaactg
      361 ggtcggggag aggatggcgt aggtctgccg ctgcttaacc tgctggccca tcatttcgta
      421 ggaagtttca ccgacgtagg ttttgtcgtc cacccagttg ccgggcgtct cctggccgtc
      481 cccaaaggaa tcaaaccacc agtgccggta acccttcttc tgcgggtcat aggtccagag
      541 ggagtgacct gcaaacttca tcgcgttctt cccctcgccc atcgacgacg tgtaatcgcc
      601 caccaggaac atgccgccga ttccgcgctt ggtgacggtt ttcgacttgt tcttgctccc
      661 cggcggcatg cccgggctgt tcatcgtttc ctcgcccgtc caggtaccga gaaagtaatc
      721 cagctttgcg agctccggtc ccggcttggg catcatcatc tccatcgacg gctccgccgg
      781 cttgttgctc ttgctctgct cctgctgtgc cacgatcgaa gtcaccgcga acaaaccgac
      841 cgcgagcacc aagagacttt tccgcattcc tcctcctttc agctgacgtg cgccaaacga
      901 ttcgcctctt tccctcaagg cgcgacaaac ctaaaaaacg agaacgcacc tgtcaagtgc
      961 ttcacttttc cttttcgccg cttgaatctc cagcttgcac aacgccccgg ttcttgacgt
     1021 gcttgtctaa gaactccgcc accgccgggt aggccttgat ccggttggcc aattttgcca
     1081 ggccgtgccc ttcatcgggg aagagcatgt attccaccgc gccgccctgc tttttcacct
     1141 ggtcgacgat ctgctccgcc tccgactgcg gcacccgcgg gtcgttggct ccctgaatca
     1201 caaagagcgg gcacttgatc ttatccacgg cgtgaatggg cgaatacttc cggaggacct
     1261 caatatcctt gtcgaggtaa ccgtactccg cctcccggag tttccgccgc caggcgccgg
     1321 tgtttttcag aaaagtctcg aagttggcga tgccgacgat atcgaccccc gccgcccaca
     1381 attcgggcag aaaagtgact gccgctaggg tcatgtagcc gccgtaactt gcccccatca
     1441 ccgccacttt ggccgggtcg acatagccgg aattcttcag aaaattatag caagctttga
     1501 cgtcctggat ggcttggccg cgcttttcga tatcatccaa gtgcgtgtag gttttcccat
     1561 agccggtgga gccgcgaatg ttgggagcca gaattgcgta cccgcgggag agatagtact
     1621 ggtaggtggt ggcaaagtag ggccgctcct gagactccgg cccgccgtgc accatcacga
     1681 tgcaaggtgc cgagccgtct tttttggcgc ctgtgggcag gtagatgaag gcggagagct
     1741 ccagcccgtc gaacgacttg aactttacca gagtcggcgc aacgaaggac gatgggggga
     1801 tcccggccag ggtgcttctg gtcacctccg tcaatttctc ccccgccatc tcgtaggtcc
     1861 agatattccc attctgtgtc ggcccgacaa aagcgaaagc catcatcttg ccgtccttgg
     1921 aaaaagtcaa accggaagga atcccatcgg ggaggtgcac ttcgctcgtc ttggcctggg
     1981 ttatcgtccc gacccgcagg ggatgttcgc ggagaaagag ccggccgtag ccgtcgatgt
     2041 tgcgcacaat ggccaggtac cggttatcat cggaaagttt cagcccgtcg acgtcgcttt
     2101 tcccgtcgtc aagaaatgtc aacttctttt ccgccacgtc gtaataggcc aagttgacga
     2161 aatcccggtc ctggtcggag gccaggtaga accccttgcc gtcattagtc cactcgatac
     2221 tctcgtacac cgccggcggt gtgtggggag tgaggtgggt gctggtgccg gtcgccgcct
     2281 cgaccaaaaa gagatcaaag tccaggttgg tgttggcgcg cgccacgacc aggtacctgt
     2341 catcgggcga ccaggccgcc acagagttgt tcgcgtccga ctggtacacc aacttcgcct
     2401 ggtcgatggc caggtcccgg acgtagacgt cgaagtaggc ctgatcccgc tcgttggccg
     2461 cataggcgat cttcttcccg tcgtgcgacc accccccgaa gctgtaaatc actttcgggt
     2521 tgttcgtcag ttgaacgatg ttctccccgc gcggggagca gagataaagc tgcgtccgct
     2581 cgtccccgcc ggtgtccttg ccgaaggcga tcttatcttc catcggggag tagacaaaga
     2641 aatcgacccc atcctcgtag aaggtcgcct gttccggcca gccgccggtc acgggaatgg
     2701 tccacccctg gtaaacgccg gtgacgttgg tcataaaggc gagcgttttg ccgtccggag
     2761 aaaaggacgg cgaattggcg tgcttgatgt tcaggtactg ggtgaactcg tacgcattgg
     2821 ccgtcgagag acccgccata aacaacagga tcgagagcgg cgcgaaccat aatttccgga
     2881 ttttcttcat tgttcctccc cgtgttctgt gttctgtgtt ccggcttcta tccttccaac
     2941 atttccacgt tggcccgcgg tacaatggcg cgtgtgccgt cggcaaactc cacctgcaat
     3001 atacgggcgt gcgattccga ctccaacttc tgcagctccg gcggcagggt cgtgaccttc
     3061 cccaggtggc cgaagtaggg ctgacggatt acccgaaccg gcgacccctc ggctaagccc
     3121 tcttcggcaa atttttcggc tgcctcgtcc tgccggatgt cgcccacgag gggaatcacg
     3181 acctccggac ggattacccc ggcccgaatc tgggtggcgc cgttgaagca ggctaacttc
     3241 ccttcatttt gtttgagcaa agtaaaggtt ctctcggcca tattgatttg accaaaccct
     3301 tcggtgacca cgagcgtaat tcctttct
//
LOCUS       DBNGZA010000206         6703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_782100, whole genome shotgun sequence.
ACCESSION   DBNGZA010000206 DBNGZA010000000
VERSION     DBNGZA010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_782100"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..683
                     /gene="dcd"
                     /locus_tag="AC16FU_04275"
     CDS             129..683
                     /gene="dcd"
                     /locus_tag="AC16FU_04275"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR2993622.1"
                     /translation="MPIKSDRWIREMAKKHKMIDPFVDRQVRQGTISYGLSSYGYDIR
                     VASDFAIFTNVNSNFVDPKKFDRGSLVPVKAKTILIPPNSFALARTVEYFRIPRNILT
                     ICLGKSTYARCGIIVNVTPFEPEWEGHATLEISNTTPLPAKIYAREGIAQIIFLESDE
                     ICEVSYKDKKGKYQAQRGVTLPRI"
     gene            complement(1298..1963)
                     /locus_tag="AC16FU_04280"
     CDS             complement(1298..1963)
                     /locus_tag="AC16FU_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993623.1"
                     /translation="MTPPAGLGAAGGGAGIGEVGAGEVDGLGVTVGVGVTGFGIAVVA
                     GVPDGFGVSVGLGATGLAVALGFGGAAFGVVGVAGLAAGDGGVAGLGAAVVVGVPAGF
                     DISAGFGATVEVTEPVGFGAFGLAGTVVAGVPEGCLGAGAAAVPVDFGAGGGLEVAAG
                     TRLPGFGVAVVAGVPVGFAVAAGFWAGFWVVAGVAVPAGLGTSVALGAAVAAGVPVGL
                     GAV"
     gene            2031..2867
                     /locus_tag="AC16FU_04285"
     CDS             2031..2867
                     /locus_tag="AC16FU_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993624.1"
                     /translation="MPGSNLKKPLTEKDIVSHKVKELEGKHEGASGTLGFFDLTFGGG
                     APLLARYNNQFRAIAWKNIFRSDGSSQRLFEPINSSLDLVGSLGLTFGRHMVSGNFSY
                     WFKMGSANVGDFVLPQPPNGTNTLVTNFPLTSEITAWGISADYQLFVLNPPGEHPLGR
                     TWSFRLGGGAGYYHASWNLWNGYGATDPTTGQYAVAPLPLTQSKFAPHLSLGGDVPVG
                     QGWVLSVDTRYLFLNFNKIGWNDPSGNFHYVEYPSQPGERIPLDLSGFRAHLGFKKYF
                     SF"
     gene            2995..4140
                     /locus_tag="AC16FU_04290"
     CDS             2995..4140
                     /locus_tag="AC16FU_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993625.1"
                     /translation="MTLPGRPFLGLGPALLCLSLAFACGQKKKNAAEENPAPASTGGP
                     SQFQDTSSIRIAPASPAPVPQAHSQSEARGLNLPGRIAFWRLGQLWVMNADGSNQRQI
                     LRFPTGDNGYGKISWAPDGKRLAFARKGLLTVNYPDGGTGNVPIYDVFYTHLDSANWF
                     FGLSDEFGSSFPDWSPDGSFVVYARDMNRATAAANLAQYQPNYQLVVEQVKNYTTRTL
                     QMPKSTQTPLQAIQPAIAPDGRRVAFSVVISSPQGGGEVVGIATDDLRGIKKSGDALL
                     SEARRTPGGYGPAWSPDGKQLAFIVTQGATQGLCLMAPDGTNQRLLVEKSDRISINSF
                     PPAWSPDGRWLAFSTADSSIYVVNVQSRETKRLTGPHDDLHPAWSKK"
     gene            complement(4174..6183)
                     /locus_tag="AC16FU_04295"
     CDS             complement(4174..6183)
                     /locus_tag="AC16FU_04295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2993626.1"
                     /translation="MDDRLQKIQKRDWQLWALALFVLFAAVSLVVFLALGTNIGQWFQ
                     KEFRMQNVLFLLLDFALLAILLGIYLVQEEVKLKKIQEHLIEEQVSSASWQHQFQELR
                     ALFEVSTVMSAKVELPEILNTICEQVNTSLGADRTSVMLYDEHKRTLSCAAAAGQKKA
                     LTVNQEVALGKSVAGWVMEHGEAVVLGDDLSHFAFAEFVPKEEKIASAICIPLVIKGK
                     RAGVLSASRFNPQRKFSPEDLKIVSIFAENAAVSIERAQLYREVKESTRQLVQTEKMR
                     ALGEMAAGVARDFNNTLAIILGRVELLMQQIQDPKYAGWLESVRHAGLHASEIVKKIQ
                     DFTRRHATKTFVSLDLNQIIRDTVELAAPKWKHEPAARGKTIHLSTGFGEISPVEGNP
                     SELKEVFMNLIFNAVEALPEGGTILFQTRLVQGQVEVTVIDNGVGMSEETKQKIFEPF
                     FTTKGPKGTGLGLSVAYGIVAHHGGEIAVDSALGKGTTVCIRLPVHKPAHEQTKVEPK
                     VPKSQELSGARIVIVNGEAPENTILTNFLLGCGHLVKSVLSGREVLDQLRRDDLDLVF
                     LDSALSKSSPFELAAEMKRTKSAPAVVLIGGWGHVRLTQDATEQGIDFVIGRPFVLDE
                     ISAVTQKALLQRRGRLLREQVIPSRTDRAPADPVIDLTKTGAEVV"
     gene            complement(6483..>6703)
                     /locus_tag="AC16FU_04300"
     CDS             complement(6483..>6703)
                     /locus_tag="AC16FU_04300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2993627.1"
                     /translation="TSPDHNAILGRVPEVEGFICANGFSGHGFMHAPAAGEAIAELIL
                     DGKTSLDISAFSIERFRNGNFAVEQNVI"
BASE COUNT         1433 a         2176 c         1767 g         1327 t
ORIGIN      
        1 ctccgacgaa gaactggcgc aactcctcgc ctacctggag acggtgaagt agatcggttt
       61 cggccggctg ttgacttccc cgccgacggg gcattatttg gctgcgaaaa aattacgccg
      121 gagcaaaaat gcccattaaa tctgaccgct ggattcggga aatggcgaaa aagcacaaaa
      181 tgatcgaccc gttcgtggac cgtcaggtgc gccagggaac gatctcctac ggcctttcct
      241 cctacggcta cgacatccgg gtggcgagcg acttcgccat tttcaccaac gtcaactcca
      301 atttcgtcga tccgaaaaaa ttcgatcgcg ggtcgctcgt gcccgtgaag gccaaaacga
      361 ttctgattcc gcccaattcg ttcgccctgg cgcgcaccgt ggaatacttc cgcatccccc
      421 ggaacatcct caccatctgc ctggggaaat cgacctacgc ccgctgcggg atcatcgtca
      481 acgtcacacc attcgagccg gagtgggaag gccacgctac cctggaaatt tccaacacca
      541 ctcctttgcc ggcgaaaatc tacgcccgcg aaggcatcgc ccagatcata tttctggagt
      601 ccgacgaaat ctgcgaagta tcctacaaag acaaaaaagg gaagtaccaa gcccagcggg
      661 gagtcaccct cccccgtatt tagtccaagg tctccttgag gttaggggag tcttaacctc
      721 gacaccccga cacctcgata cttcgacact cacttaagtt tttccccttt ccgtccgatt
      781 taatagcaga tgccaaattg gacaaggagg ggagtatgag acggctgtct cttttgtttt
      841 tggctgtgag tctggtggct ttctcggccg gaaccgtgat ggcccagacc accgaagatg
      901 acatggtcag acgcttcatc gaagagaaag aaaaagcgca gaaagagaag aacaagaaga
      961 agaaaaagac cgcgccccaa aaagaggcca ccgccaagca agccgaaaca gcctcgaccg
     1021 tcgatacgtc gaagaacaag gggacgggtc agaccgccgg aacgaccgcc caacccgccg
     1081 caggcggaca acccgctacg acacgaccga ccgggacggc tccggcaacg gtaacgccaa
     1141 aaccggcctc accaacaaat cctcctccgc cagcgcctat caaaccgggt ctccaaccgc
     1201 cgaaaacaac tgcaactccg gcgccgccga aaccgtcaac agcgccggcc aaaccagccg
     1261 taccgaaacc gggtgtgcca aaagccggag ccccaactca aaccgcgccc aagccgaccg
     1321 gtactccggc tgccacggcc gcacccaaag cgactgaagt acccaaacca gccgggaccg
     1381 cgactccagc tacaacccag aaacctgccc agaaacctgc ggcaacggcg aagccgaccg
     1441 gcaccccggc cacaacggca actccaaagc cgggtaacct ggtgccggcc gcgacctcca
     1501 atccaccgcc cgcaccgaag tcaactggca ccgccgcggc acctgcgccc aaacatcctt
     1561 ccggcacgcc ggcaactaca gttccggcca agccgaatgc gccgaaaccc accggttcgg
     1621 taacctcaac cgttgcgcca aaacctgccg agatgtcgaa gccagccggt accccgacga
     1681 cgacggcggc tcccaaaccg gcgactccgc cgtcaccagc cgcaagtccg gcgaccccaa
     1741 ccactccaaa ggcggctccg ccgaagccaa gcgcgacagc gagacccgtg gcgcccaaac
     1801 ctacggaaac accgaaaccg tccggcacac cggctacgac cgcaattcca aagccggtca
     1861 ccccgacgcc aactgtgact cccaaaccgt cgacttcacc tgcgccaact tcaccaatcc
     1921 cggcccctcc gccagccgca cccaagcctg ccgggggcgt cacaccagcc ccagcaccgg
     1981 cccctgtcca ggccacacca ctggctccgc gcaagcccga ccagggtatt gtgcctggct
     2041 caaacttaaa gaagccctta acggaaaaag atattgtatc ccataaagtc aaagagctcg
     2101 aaggcaaaca cgaaggagca agcgggacgc tcggattttt cgaccttact ttcggtgggg
     2161 gcgcaccgct gttggcccgc tacaacaacc agttccgggc catcgcctgg aagaacatct
     2221 tccgttccga cggctcatcg caacgcctgt ttgagccgat caactccagc ctcgacttgg
     2281 tcggcagtct cggcctgacc ttcggacggc atatggtttc cgggaatttc tcgtactggt
     2341 tcaaaatggg ttccgccaat gtgggcgact tcgttttgcc ccagcccccg aacggaacca
     2401 acacgctggt gacaaacttc ccgttgacca gcgaaattac cgcctggggt atctccgccg
     2461 actaccagct cttcgttctg aatcctcccg gcgagcatcc tctgggccga acgtggtctt
     2521 tccggctggg cggcggtgcg gggtattacc acgcctcctg gaatctttgg aacggctacg
     2581 gggccacaga ccccaccacc ggccagtatg cggtggcacc cctgccactc acgcaaagca
     2641 agtttgcccc ccacctgagt ctgggcggcg acgtccccgt cggccagggc tgggtgctct
     2701 cggtggacac gcgctatctg ttcctgaact tcaacaaaat tggctggaac gacccttccg
     2761 gcaatttcca ctacgttgag taccccagcc agccgggcga gcggattcca ttggatctga
     2821 gcggcttccg ggcgcatctc ggatttaaga agtacttctc cttctaaacg cgggagtttt
     2881 tcctccgcaa gggtagacgg gctcgtctcc gagcccgtct tcttttaccc atcagcttgt
     2941 acccgaaatt ttgttgttgt gctgctcccc ggttcgacta tatttgccgc cgcgatgact
     3001 cttccgggac gacctttcct cggtctgggc ccagcgttgc tgtgcctctc tctcgctttc
     3061 gcctgcggac agaaaaagaa aaacgcggct gaggagaatc ccgctccggc ttccaccggc
     3121 gggccgtcgc aatttcagga cacttcaagc atccgcatcg ctccggcttc acccgcaccg
     3181 gtgccgcagg cgcacagtca aagtgaggcg cgcgggttaa acctccccgg ccgcatcgcc
     3241 ttctggcggt tgggccagct ctgggtgatg aatgccgacg gctccaacca gcgccagatc
     3301 ctccgctttc cgaccggcga caacggctat ggcaaaatct cctgggcacc cgacggcaaa
     3361 cggctcgctt ttgctcgtaa gggactttta acggttaact accccgatgg cggtacgggc
     3421 aacgttccca tctatgacgt tttctacacc catctcgatt cagccaactg gtttttcgga
     3481 ctctccgacg aattcggttc cagctttccc gattggtcgc cggatggttc tttcgtcgtc
     3541 tacgccaggg atatgaaccg ggccaccgcc gccgcgaacc tggcgcagta ccagcccaac
     3601 taccagctcg tggtggaaca ggtaaaaaac tacaccaccc gaacgctcca gatgccgaaa
     3661 tctactcaaa cgccgctgca ggcgattcaa cccgccatcg ccccggatgg ccgccgtgtg
     3721 gccttctccg tggtcatttc cagcccacaa ggtggcgggg aagtggtcgg gattgccaca
     3781 gacgatttgc gcggtatcaa gaaaagtggc gacgcgcttt tgagtgaagc acgccggacg
     3841 cccggcggct acggcccggc ctggtcgccc gatgggaaac agctcgcctt tatcgtcacc
     3901 cagggggcaa ctcagggcct ttgtctgatg gccccggatg gtaccaacca gcgcttgctc
     3961 gtggaaaaaa gcgaccgcat ttcaatcaat agttttccgc ccgcctggtc cccggatggc
     4021 aggtggctcg ctttttcaac cgctgactcg agcatctacg tcgtcaacgt ccagtcgcgc
     4081 gaaaccaaac ggcttaccgg cccgcacgac gacctgcacc ccgcctggtc aaagaagtag
     4141 tcggctttct ggggtggaac gaagttccgg aggttaaact acctccgctc cggtcttcgt
     4201 gagatctatg actgggtccg cgggagccct gtcggtccgg gagggaatga cctgctcgcg
     4261 gaggagccgg ccccgcctct gaagcagggc tttctgagta acggccgaaa tctcatccag
     4321 cacaaacggc cgtccaatca caaaatcgat cccctgctcg gtagcgtcct gggtcaagcg
     4381 gacgtgtccc caccccccga tgagaaccac tgcgggtgca cttttcgttc tcttcatttc
     4441 ggcggcaagt tcgaaaggtg acgatttact cagggcggag tcgaggaata cgagatccag
     4501 gtcgtcccgc ctcaattggt cgagtacctc ccgtcccgaa aggacacttt ttaccagatg
     4561 accgcaaccc aaaaggaagt tcgtcagaat cgtgttctcg ggtgcctcgc cgttcacgat
     4621 cacgatgcgt gcacccgata attcctggct ttttgggact ttcggctcca ccttcgtttg
     4681 ttcgtgcgcc ggcttgtgaa cgggcaaccg gatgcaaact gtggttcctt tgccgagagc
     4741 ggaatcaacg gcgatctcgc ctccatggtg cgccacgatg ccgtaggcga cactcaggcc
     4801 gagaccggtc cccttggggc ctttggtggt gaagaacggt tcaaaaatct tctgtttggt
     4861 ttcttcgctc atgcccaccc cgttatcgat cacggtcacc tcaacctgcc cctgcaccaa
     4921 ccgggtttgg aagagaatgg tgcctccctc ggggagtgcc tccactgcat tgaaaatcag
     4981 gttcatgaac acttctttga gttccgacgg gtttccctca actggggaga tttcaccgaa
     5041 gcccgtggag aggtgaatgg ttttcccgcg cgcggctggt tcgtgtttcc atttcggcgc
     5101 ggcgagttcg acggtgtccc ggatgatctg gttgagatct aaggatacga acgttttggt
     5161 tgcatggcgc cgcgtgaaat cttgaatttt cttgacaatt tcgctcgcgt gcaggccggc
     5221 gtgccggacg ctctccagcc atcccgcgta cttaggatcc tggatttgct gcatcaagag
     5281 ctcgacccgc ccgaggatga tcgccagtgt attgttgaaa tcgcgggcga ccccggccgc
     5341 catctccccc aaagcccgca tcttttccgt ctggaccagc tgccgcgtcg attctttgac
     5401 ctcccggtac agctgcgctc gctcaatgga gaccgcggcg ttctcggcaa agatggaaac
     5461 gattttcagg tcttccgggc tgaatttccg ctgggggtta aaccgcgacg cgctgagcac
     5521 ccccgcccgc ttccctttga tcacgagcgg gatgcagatg gctgaggcga tcttctcctc
     5581 tttgggaacg aactcggcaa acgcgaagtg cgaaaggtca tcgcccagaa ccaccgcctc
     5641 gccgtgctcc atcacccagc ccgctaccga cttccccagg gccacttcct gattcaccgt
     5701 cagggccttc ttctggccgg ctgcagccgc gcagctgagc gtgcgtttgt gctcatcgta
     5761 gagcatgacg ctcgtgcggt cggctcccag actcgtgttc acctgttcac aaattgtatt
     5821 caggatttcc ggtaattcaa ctttggcgct catgaccgtg gagacttcaa agagcgcccg
     5881 cagttcctga aactggtgct gccacgacgc cgaactgacc tgttcctcaa tcaggtgctc
     5941 ctggattttt tttaatttta cctcttcctg caccaggtag atgcccaaaa ggattgccag
     6001 gagggcgaaa tccaaaagga ggaacagcac attctgcatc cggaattctt tttgaaacca
     6061 ctggccgatg ttcgtgccaa gcgccaaaaa gacgaccaga gagacggccg cgaacaggac
     6121 aaaaagtgcg agcgcccaaa gctgccagtc gcgcttctgg attttctgca aacgatcatc
     6181 catggatttg ttccgattct ctcagtctct ctgtatatcg gcatattctc cctgacgatg
     6241 aacaatccgg aggcgtcacc atagtgtcct gcattttccc ccgctttcag cgcctccggc
     6301 cggtgcgtcg cgctccccag ctggtcaatg ggatttcccg tctttcaacg actgcgttgc
     6361 gctccccggc tacagccggt tgagtcggaa ccgagaaaac ccgcaggcat ctttgcgggc
     6421 tctcgaaaaa tcagaagggg aaccgtcttt ggatttgacg gtgcgcctct gccataaagt
     6481 gcttagatga cgttttgctc gacagcgaag tttccgttgc ggaagcgctc aatggaaaaa
     6541 gcggagatat ccagagacgt tttgccgtcc aggatcaact ccgcaattgc ttcccccgct
     6601 gccggagcgt gcataaagcc gtggcccgaa aaaccgttgg cgcagatgaa cccctccacc
     6661 tccggtaccc tgcctaagat ggcgttatga tcgggagagg tct
//
LOCUS       DBNGZA010000207         1829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2373051, whole genome shotgun sequence.
ACCESSION   DBNGZA010000207 DBNGZA010000000
VERSION     DBNGZA010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2373051"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="AC16FU_04305"
     CDS             complement(<1..1463)
                     /locus_tag="AC16FU_04305"
                     /inference="COORDINATES: protein motif:HMM:NF015019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0098655 - monoatomic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proton/sodium-translocating pyrophosphatase"
                     /protein_id="HIR2993628.1"
                     /translation="MKTFTIFLTSLIFLTLATSVWAGEGDVKLPPFDKTINTSLYFVL
                     LSAVIALLYGLYLVRKTLAREQGTAKMIEVGRAIQEGAKAYLTRQMKVLSLFIILLTV
                     VIFFVYKGLYLLEDGTTNWKLVWGIALAFLAGSSLSAMTGLIGMSVAVRSNTRVAHAA
                     RTSFKQALESAFEGGTVTGMFTVGMGLVGATIIFMLFKEQAMQVLIGFGFGGSLVALF
                     MRVGGGIYTKAADVGADLVGKVEAGIPEDDPRNAATIADNVGDNVGDCAGMAADLFES
                     YEVTLVAAMILGAAYSALHPEQSGLALKMIVFPLLVRAVGVFASIFGTWSVRGKDRDD
                     FNPMRPIQNGFVVSAVTATLGFGLINYFFIKTPDGSADFRFFWATFSGIILAVVISYL
                     TEYYTALEYSPVKETARATKTGPATTILAGFSEGLESTVVAIMAIAVAIVSSALIFKG
                     DLALSAYWIALCGMGMLTNTGVIVSMDSYGTISDKANG"
     gene            complement(1636..>1829)
                     /locus_tag="AC16FU_04310"
     CDS             complement(1636..>1829)
                     /locus_tag="AC16FU_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2993629.1"
                     /translation="ARRPLGIAVVGGILFSTFLTLVLVPVVYTLLARFTRREAAKGET
                     PEETPATSEPHLSPAAVTG"
BASE COUNT          437 a          534 c          522 g          336 t
ORIGIN      
        1 ccgttggctt tgtccgaaat cgtcccgtag gaatccatcg agacgatgac gccggtattg
       61 gtgagcatcc ccatcccgca gagcgcgatc cagtaggccg acagggcgag gtcgcctttg
      121 aaaatgagag ccgaggacac aatcgccacc gcaatggcca tgatcgccac caccgtcgat
      181 tccagaccct cggaaaaacc ggccagaatg gttgtggccg gaccggtctt ggtagcccgc
      241 gccgtctcct tcaccggact gtactccagg gccgtgtagt actcggtgag gtaactgatg
      301 acgaccgcga ggataattcc ggagaaggtg gcccagaaga agcggaagtc ggccgacccg
      361 tccggggttt tgatgaagaa gtagttgatg agtccgaagc cgagggtggc ggtcaccgcc
      421 gagaccacaa aaccgttttg aatcggccgc atcggattga aatcgtcccg gtctttgcct
      481 ctgacgctcc aggtgccgaa aatggaagcg aaaacaccca ccgcccgcac taacagggga
      541 aagacgatca ttttcaaagc caggcccgac tgctccggat gcagggcgga gtaggcggcg
      601 cccaagatca tcgcggcaac gagcgtgacc tcgtacgatt cgaacaggtc ggcggccatc
      661 ccggcgcagt cgcccacgtt gtcgccaacg ttgtcggcaa tggtggccgc gttgcgcgga
      721 tcgtcttcgg gaatgccggc ttcgactttg ccgaccagat cggcccccac gtcagccgct
      781 ttggtgtaaa tccccccgcc cacgcgcatg aagagcgcca cgagagaccc gccgaaaccg
      841 aagccgatca aaacctgcat cgcctgctct ttaaagagca tgaagatgat ggtggcgccg
      901 accaacccca tcccaacggt gaacataccg gtaaccgtgc cgccttcgaa agccgattcc
      961 agcgcctgct tgaatgatgt ccgggcggca tgcgccacgc gggtattgga gcgcaccgcc
     1021 accgacatcc caattaagcc ggtcatggcc gaaagagatg aaccggccaa aaaggccagc
     1081 gcaatgcccc agaccaattt ccagttcgtc gtgccgtctt ccaagaggta aagccccttg
     1141 tagacgaaga aaatcaccac cgtcaaaaga atgatgaaaa gcgacaacac tttcatttgc
     1201 cgggtgaggt aggccttggc gccttcctgg atggcgcgcc ccacttcgat cattttggcc
     1261 gtcccctgtt cgcgggccag cgttttgcgc accaggtaca ggccgtacag gagggcgata
     1321 actgccgaca ggagaacgaa atagagcgag gtgttaatgg ttttgtcgaa aggcggaagt
     1381 ttgacgtccc cttcccctgc ccaaaccgaa gtggcaagag tcaaaaagat aagagaagtc
     1441 aaaaagattg tgaaagtttt catttggaaa aagcggcctt tcctccactg aagctgcatg
     1501 cacctctcct tctttggtta cccgctctgc cccaagtttc gtaatctcta cagcgagcag
     1561 aatataggaa cggttttctt atatgcaagt caaaattggt cttgctcgaa gagtaaagaa
     1621 accgcccgac ggggctcatc cggtcacggc agcgggactg agatgcggtt ctgaggtagc
     1681 gggagtttct tcgggagttt cccccttcgc cgcttcccgc cgggtgaagc gcgccagaag
     1741 cgtataaacc acgggaacca agactaaagt caagaacgtg gaaaacagga tgccgcccac
     1801 cacggcaatg cccagcgggc ggcgggctt
//
LOCUS       DBNGZA010000208         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1312343, whole genome shotgun sequence.
ACCESSION   DBNGZA010000208 DBNGZA010000000
VERSION     DBNGZA010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1312343"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="AC16FU_04315"
     CDS             <1..918
                     /locus_tag="AC16FU_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2993630.1"
                     /translation="SRKGNWVGGFDLLVDEFGGARTFGIRWARKSLKTSEVNLAYTEE
                     NPWAWPLDFTFHLAQQQRDTLYFQLAVSGALNWEVLTRLTLGLKSGWEKVVPGDRAPL
                     VVFPSRKFSLGGEVTYDGTDDLFNPARGIRLSVASMVTRKRSFVLHGVAPEKPLLYQR
                     STTLDLTQYLPISRVTQVFFRLHGAGIGSSERVVPLADQFFLGGQGSVRGYQKDQFSG
                     SRVAWTNLEYRLRFGEGDRVFLFTDAGYYYRREQSPGEIIFREATQVGFGLGLAVRRS
                     FGEFEVSVGWPQHAAVGDGIVNFGVVNRF"
     gene            964..1767
                     /locus_tag="AC16FU_04320"
     CDS             964..1767
                     /locus_tag="AC16FU_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HIR2993631.1"
                     /translation="MNVVITFASVGLGAYLTGSGVDWLSIAVAACTAGLICAGGNALN
                     DLCDIGCDQINKPLRPLPSGRLSPKAVRSFVVLLFTSGVILAASFPAPVLGIAVGVVM
                     LLVLYNFRLKRLPLVGNALVSLAAAASFPYGALAAKKEVVPEVWFITLFAFLFHFGRE
                     ILKDLEDLPGDLKVNARTFPIRYGLVPTRRLVAAVFFVLALSTLIPALLKLFSWRYTV
                     VVVGGVDLSLLYIVWSLARDASSLNLHRLSRLLKVDMVVSLLAVFLGVQ"
     gene            1850..>2609
                     /locus_tag="AC16FU_04325"
     CDS             1850..>2609
                     /locus_tag="AC16FU_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2993632.1"
                     /translation="MHQDIKAISELVEKESVAVRQLVAEIGKVIVGQKYMVERLLVGI
                     LANGHILVEGVPGLAKSLTVKTLADTIKAKFQRIQFTPDLLPADLIGTMIYQPGDGKF
                     TTKKGPIFANLILADEINRAPAKVQSALLEAMQERQVTIGEKTYPLDEPFLVMATQNP
                     IEQEGTYPLPEAQVDRFMLKLSVGYPNKEEDLEILNRMTVGNSFNADAVVSPEEILRG
                     RKVIHQIYIDDKVKEYILNIVFATRQPETYGLKLK"
BASE COUNT          523 a          695 c          763 g          628 t
ORIGIN      
        1 tcgcgcaagg ggaattgggt cggcggattc gacctgttgg ttgatgaatt cggcggggcg
       61 cgtaccttcg gaatacgctg ggcccggaaa agtcttaaaa cctcggaagt caacctggct
      121 tacaccgaag agaacccctg ggcctggccg ctcgatttta cctttcatct cgcccaacag
      181 cagcgcgata cgctgtactt tcaactggcg gtctcgggtg cactcaactg ggaagtgctg
      241 acccgtctga cgctggggtt gaaaagcgga tgggagaagg tggttcccgg agacagagcc
      301 ccgcttgtcg tctttcccag ccgcaaattt tctctgggag gtgaagtgac gtatgacggc
      361 acagatgatc tgttcaaccc cgcgcgcggc atccggcttt ccgttgcaag tatggtcact
      421 cgcaagcgga gttttgtgtt gcacggcgtt gcaccagaaa aacccctcct ttaccagcgt
      481 tcgacaactc tggatctgac gcagtaccta ccaatctcca gagtgacgca agtttttttc
      541 cgattgcatg gtgccggcat cggttccagt gagagagtag ttccgcttgc ggatcagttt
      601 ttcttaggcg gccaggggag tgtgcggggg taccaaaaag atcagttttc gggcagccgg
      661 gtggcctgga ccaatctgga ataccggctt cggtttggtg agggagaccg ggtttttctt
      721 tttaccgatg ccggatatta ttaccgacgt gaacaatcac caggtgagat tattttccgg
      781 gaagcgacgc aggtcggctt tggtttgggt ctggcggtga ggcgttcgtt cggagagttc
      841 gaggtctcag tcgggtggcc ccagcacgcg gctgtgggcg atgggatagt gaacttcggc
      901 gtcgttaacc ggttttagac aatgctggca ctggcttagg cgttttggga aatcagccgt
      961 ccggtgaacg tcgtcatcac ctttgcatcc gtcggtctcg gtgcctatct gacgggaagc
     1021 ggggtggact ggctttccat tgcggtagcc gcgtgcactg ccggattaat ctgcgccggc
     1081 ggcaatgccc tcaatgacct gtgcgacatc ggctgcgacc aaatcaataa accgctccgg
     1141 ccgttacctt cggggaggtt gtccccgaaa gccgtccgct cgttcgtggt gctcctcttc
     1201 acatccggag taatcttggc ggcttctttt ccggctccgg tgttgggaat tgccgttggc
     1261 gtcgtcatgc tcttggttct ttacaacttc cggctcaagc ggctgccctt ggtgggcaac
     1321 gccttggtca gcctggccgc agcggccagt tttccctacg gggcgctggc ggccaaaaaa
     1381 gaggtcgttc ccgaagtttg gttcatcact ctttttgcgt ttctctttca ctttgggcgg
     1441 gaaattttga aggacctcga agacctgccg ggcgatctga aagtgaacgc ccgcaccttt
     1501 cccatccgct acggtttggt gccgacccgg cggctggtgg cagcagtctt tttcgttctg
     1561 gcgttgtcta ctctcattcc ggcactgctc aaactttttt cctggcgtta caccgtagtc
     1621 gtcgtcggcg gagtggacct ttccctcctg tacattgtct ggtcgctggc ccgggatgcc
     1681 tccagtctca acctgcaccg gttgtcccga ctgctcaagg tcgatatggt ggtgagtctt
     1741 ctggcggtct ttctgggtgt acagtaacct tctccacttg accctctcga aaattgccaa
     1801 catattggtt ttatctccga ataatagaga ttgaagaact ggaggtcgga tgcaccagga
     1861 catcaaagcg attagtgaat tagtcgaaaa ggaatcggtg gcggtccggc agttggtggc
     1921 cgaaatcggc aaggtgatcg tcgggcagaa gtacatggtg gagcggctct tagtcggcat
     1981 tttagccaac gggcatattt tggtcgaagg ggtacccggg ctggccaaaa gtctcacggt
     2041 gaagacttta gcggatacca tcaaggccaa gttccagagg attcaattca cccccgacct
     2101 tttgcccgcc gacctcatag gcacgatgat ctatcagccc ggcgacggca aattcaccac
     2161 caagaaaggc cccatctttg ccaacctgat tttagccgac gaaatcaacc gcgcgccggc
     2221 caaggtgcag tcggcccttt tggaagcgat gcaggagcgg caggtgacca ttggcgagaa
     2281 gacctacccg cttgatgaac cgtttttggt gatggcgacc cagaacccga ttgagcagga
     2341 aggtacttat cccctcccgg aagcgcaggt agaccgcttt atgctcaaac tctcggtggg
     2401 ctacccgaat aaggaagagg acttggaaat cttgaaccgg atgaccgtcg ggaactcctt
     2461 caatgccgac gccgtggtgt cacccgaaga aatcctgcgg gggcggaagg taattcacca
     2521 gatctacatc gacgacaagg tcaaagagta cattttgaac atcgtctttg ccacccgcca
     2581 gccggaaaca tacgggttga agctgaaag
//
LOCUS       DBNGZA010000209         6596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1523089, whole genome shotgun sequence.
ACCESSION   DBNGZA010000209 DBNGZA010000000
VERSION     DBNGZA010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1523089"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="AC16FU_04330"
     CDS             <1..841
                     /locus_tag="AC16FU_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2993633.1"
                     /translation="SQTGGAFGRVDSIKMVLPVAYPPAFDLKGVNGTDPDSLFILWVD
                     PYGGYIPGSSQRREAFQLFVTNNANVGVYSLDSNRLSSAKTLKVCHIISLPCLVTFVR
                     GVETILNDPPLLRCSLGRQVYFGASVSLLPDEASDPNGDPLTFSLVSFVKAGGGSPPQ
                     YAPVVDSTTGRFFWRSAFDVTELGAWEVTIGVMDPFPRPPVTCSFTIEVADLRGDVNN
                     DGLWSDLVDVLELINAVAFQSPITSENPNAADVNCDGMTDMVDIIDLINFVVFGTPMP
                     CRG"
     gene            1154..1483
                     /locus_tag="AC16FU_04335"
     CDS             1154..1483
                     /locus_tag="AC16FU_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993634.1"
                     /translation="MGAKKRGWIKWVAGTLAGVCLLAAPLWGGPPVTPYDTDDHPWEG
                     NPYGPPTSGGGHGIVIIVKGSGPWVGVPYFWNIAVIKTQGTSTRTASQSASGGKGSAR
                     VEKKAGR"
     gene            1490..4399
                     /locus_tag="AC16FU_04340"
     CDS             1490..4399
                     /locus_tag="AC16FU_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2993635.1"
                     /translation="MQVRPAPETIRSERLAHIAQLVGLRRFTEAIAEIRDFDQSASGG
                     AGKVSPAEWAEACFYAAQAFHSVGNYTEALSRAEAAYEYYKPTSENEKIARLQFLLGL
                     IYLGLGKLPEAEAELRDAYSTSRRIGDMAGMIDALNRQANIAFLRFDHAQAISELTTA
                     IELARGTDDRARSAWLTGNLGRIHLFLGERSKAIECLRVSLEHNRATGDEPNLSRTQL
                     SLGFAYLLSRNLAVATEFLLSARELIEKHNLSRELGIFHEYYGQLLMEKGDFAAARRE
                     YEAGIAIAEKIGPESNLASQLYRLYAECAFEQGDYDLARSYCERGMSVATRLNERIEL
                     GVLHRLSARLALVAQSPAEAERHLRESIAIFEQIGVKHELAQTLIVAAESGLFAPQVV
                     ADQFLRAEKLFAELNDREGLFAACRREIELFGKQEDYDRALDAIARAETDARRLLELR
                     EATAFQTLRRKIEERVAELATSSANEYHFFSPAFAGGSPTEMNQVAEAMLLLMQPVQA
                     TCGILALLEKTPRSQSTAAEAARFSVVWQKDIPAALSPHLEAVFDRVQESRKPAWYSD
                     CRTESAFGGLRSAIVIPFTWARERLAVILLGIHDSAAGPVRQFAKTDFFFAAAFAEFV
                     ALRLVDEERRHLEDENRRLRRQLEERCTFTSVVTQDPALLGMLDRLELVKDSELPILL
                     EGETGTGKDLVAKAVHYASKWKTGSFVPVSCSALPETMLESELFGHKKGSFTGAATDK
                     VGLIEEADGGTLYLDEVAEIPLSIQVKLLRVLEEKELVRLGETRPRKVNCRIISSTNR
                     DVRQAVTEGRFRKDLYYRLNAVNVVFPPLRQRKEDLPLLIDHYLTKLSRNESLPTGNG
                     IPVRLAAEVLRAFYHYDWPGNVRELVNELSRILVLAKGVPVIGAEYLSDKFTSAAASP
                     LLPGSLETRLSQYERDLIEKALSASDRVKAQAARILGIPVTTLRYKMGKHGLNWK"
     gene            complement(4905..5276)
                     /locus_tag="AC16FU_04345"
     CDS             complement(4905..5276)
                     /locus_tag="AC16FU_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993636.1"
                     /translation="MRTIGEFYREEVLPHKPLSKRQLPTQRDKSEIVKDLFGWKLYSG
                     KEFIDCRSEEEARFLKVFLDAGMQEVKVPKDDRILKEILPKLERLKRNIDEIIEEHAD
                     GLLSPRLKEELRHRVWQELVS"
     gene            complement(5273..>6596)
                     /locus_tag="AC16FU_04350"
     CDS             complement(5273..>6596)
                     /locus_tag="AC16FU_04350"
                     /inference="COORDINATES: protein motif:HMM:NF046985.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993637.1"
                     /translation="CDKKIERDANLCRFVYLKKPLTHFIPPAGDIWAQELSRLRAIDN
                     LINTILSHDSLYENEELRIFPKSQKELWEEGVEIEEDGGQEHTGKEKKENYVGSKWGR
                     YLRAPEIFFEILEKAKGKIVPFRQIANIKFGIKSGADDFFYLSKEDLLHLQLTPKYVK
                     PIIVSLDETESIVIRVAKLPKSVFWANEEKSKLPKSILQYIRQGENRTFSKGKRSYIP
                     AKTKTCSSRPLWYSLGERDPAPILHPLYMGDRFLILFNREGVLTNQNLHEVYPLYKED
                     DEAEVLCAIMNSIVFQLCHDLHGRNLTGAINVIGVEMGLLSQVPIIDTAQIPSSTRLD
                     ILRAFRKLSVRPISHILDELGVFSGGNVSFNTAKPDRRELDKIIMRDILGLKEEEQLE
                     VYKAVVDLVRSRIEKAQSVEKGKRYIEGVDVDALAGGVVKQVKGKKEK"
BASE COUNT         1452 a         1849 c         1768 g         1527 t
ORIGIN      
        1 gtcgcaaact ggcggagcgt tcggaagagt tgattccatc aagatggttc tgcccgtagc
       61 ttatccgccc gccttcgatt tgaaaggagt aaatggaacg gatccagact ctctattcat
      121 actctgggtt gatccttatg ggggttacat cccaggtagt tcgcagcgcc gtgaggcatt
      181 tcagctgttt gtgaccaata acgccaatgt aggggtgtac tcgctggact ctaatcggct
      241 ctcctccgcc aaaaccttga aggtctgtca cataataagc ctgccttgtt tggtgacatt
      301 cgtccgcgga gtggaaacga ttctaaacga ccctccgctc ctgcgctgct ctctcggtcg
      361 gcaagtctac tttggagcgt cggtctctct tttgccagac gaagcgagcg acccaaatgg
      421 tgatcccctc accttttcat tggtcagttt cgtgaaggca ggtggaggca gtccgccaca
      481 gtacgctccg gtcgtagaca gcaccaccgg tcgctttttc tggcgtagtg ccttcgatgt
      541 gacagagctt ggcgcgtggg aggtaactat cggcgtcatg gaccctttcc cgcgtcctcc
      601 ggtcacctgt agcttcacca tagaagtagc ggaccttcgc ggagatgtaa acaatgacgg
      661 gctgtggagc gatctggtgg atgtgcttga actgattaac gctgtcgcat tccagtctcc
      721 cataacctcg gaaaatccta atgcggcaga tgtgaactgt gacggaatga ccgatatggt
      781 ggacattatc gaccttatca atttcgtggt gttcggaact ccgatgcctt gccggggata
      841 ggtcttgcac ttacccgcac acaggagtta tcttaccagc gtatacttac ctgcaagaaa
      901 tctcttgact ttttggagga tggcgggtat tttttcttct gagggtgggg gtatgaagct
      961 ggaagcgggc aggttcgccg cctgctcgac ggatatatcg gtcgtgcggg aggcgcccgc
     1021 actgcaaaag aagttcgcca caaggcattg aaaatgaaga agatgccacg tccgccacac
     1081 gtcaggcaag tcaccggcgg ttcgtatcga gggccgcatg gttgggagcg gtccaagtaa
     1141 aggagtccgg cttatgggcg cgaaaaaacg agggtggatc aagtgggtgg caggtactct
     1201 ggcgggagtc tgccttttgg cggcgccgct ctggggcggg ccaccggtta ctccatatga
     1261 cactgatgac catccttggg aagggaatcc gtacgggcct ccgacgagcg gcggcggcca
     1321 cggaatcgtg attatcgtca aaggtagtgg cccgtgggtc ggtgtcccct atttctggaa
     1381 catcgccgtg atcaaaaccc agggcacttc cactcgaacc gcgtcccaat ccgcctctgg
     1441 cggaaaagga agcgcgagag tcgagaaaaa agcaggtcga tagccgacta tgcaagtacg
     1501 ccccgcgccg gagacgattc gatccgaacg tctcgcgcac attgcccaat tggtcggcct
     1561 gcggcggttc accgaagcga tcgccgaaat ccgagacttt gaccagtccg cctccggcgg
     1621 agcaggcaag gtgtctcccg cggagtgggc ggaagcctgc ttttacgccg cccaggcctt
     1681 tcactccgtg gggaactaca ccgaggcctt aagccgggca gaggccgcct acgagtacta
     1741 caaaccgacc agcgaaaacg agaagattgc ccgactgcag tttcttttgg gcttgattta
     1801 cctcggattg gggaaactcc ccgaggccga ggcggagttg agggacgcct actcgacgtc
     1861 ccgccgtata ggcgatatgg cgggaatgat cgatgctttg aaccgccagg ccaacattgc
     1921 ttttctccgc ttcgatcacg cgcaggcaat ttcagagctt accacggcca tcgaactggc
     1981 gcgcggcacc gatgatcgcg cccggagcgc ctggctcacc ggcaatctgg gccgcattca
     2041 cctcttttta ggcgaaaggt cgaaagccat cgaatgcctg cgggtgagtc tggagcacaa
     2101 ccgggcgacc ggcgacgagc ccaacctttc ccggacgcag ctttcgctcg gttttgccta
     2161 ccttctgtcg cgcaacctgg cggtggccac tgaatttctc ctttcggccc gggagttgat
     2221 cgaaaagcac aacctttcgc gcgaactggg cattttccac gagtactacg ggcagttgtt
     2281 gatggagaaa ggcgacttcg ccgccgcccg gcgcgagtac gaagccggaa tagccattgc
     2341 cgaaaagatc ggcccggaat cgaatttggc ctcccagctc taccgcctgt acgcagagtg
     2401 cgcattcgaa cagggggatt atgacttagc ccggtcgtac tgtgagcggg ggatgtcagt
     2461 cgccacccga ttgaacgagc ggatagaact gggcgtcctg caccgtcttt ccgcccggct
     2521 ggcgctggtg gcccaatctc cggctgaggc cgagcgccac ctgcgcgaaa gcatcgccat
     2581 tttcgagcag atcggggtca agcacgagct ggcccaaacg cttatcgtcg ctgcggagag
     2641 cgggctgttc gctccgcagg tcgtcgccga tcaattcctg cgagccgaaa aactttttgc
     2701 agagctgaac gatcgggaag gactcttcgc cgcctgccgc cgcgaaatcg aactctttgg
     2761 gaaacaggaa gactatgatc gcgccctgga tgccattgcc cgggcggaaa ccgatgcccg
     2821 gcggcttttg gaactccggg aagcgacggc gttccaaacg ctccgccgca aaatcgaaga
     2881 gcgggtagcc gagctcgcca cctcctccgc gaacgaatac catttctttt cgccggcgtt
     2941 tgcgggcggc agtccgaccg aaatgaatca ggtggccgag gcgatgctcc tcttgatgca
     3001 gccggtgcag gcgacctgcg gcatcctggc gcttttggaa aagacgccgc gttcccaatc
     3061 gaccgcggct gaagccgcac gattcagtgt cgtctggcag aaagatattc ccgccgcgct
     3121 gtcgccacac ctggaggccg ttttcgaccg cgtgcaggaa tcccggaagc cggcctggta
     3181 ctccgactgc cggactgaat cggcctttgg cggactgcgc tcggccatcg tcattccttt
     3241 tacctgggcg cgggagcggc tggcggtcat tcttttgggc atccacgatt cagcggcagg
     3301 gccggtgcgc cagttcgcca agaccgattt cttctttgcg gcggcctttg ccgaattcgt
     3361 ggccttgcgg ttggtcgacg aagagcgccg gcacttagaa gatgaaaacc gccggctgcg
     3421 gcggcagctg gaagagcgct gcacctttac cagcgtggtg acgcaggatc ccgcgctttt
     3481 ggggatgctc gaccggctcg agttggtaaa agattccgaa ctccctatcc ttttggaagg
     3541 ggagaccgga accggcaaag atctcgtggc caaagcggtg cattacgctt ccaagtggaa
     3601 aaccggcagc ttcgtgccgg tgagctgttc cgcgctgccg gaaacgatgc tggaatcgga
     3661 actcttcggg cacaagaaag ggtctttcac cggcgcggcc accgacaaag tcgggctgat
     3721 tgaagaagcc gacggcggca cgctctactt ggatgaagtg gccgaaatcc cgctctctat
     3781 tcaggtgaaa ctcctccggg tgctggagga aaaagaatta gtgcgcttag gcgaaacccg
     3841 gccgcgcaag gtcaactgcc ggattatttc ctccaccaat cgagatgtcc gccaggcggt
     3901 gaccgagggc cggttccgca aagacctcta ctaccggctc aatgcggtca acgtcgtctt
     3961 tccgcccctg cgccagcgca aagaagatct gcccctgctc atcgaccact acctgaccaa
     4021 actctctcgg aacgagtcgc tgccgaccgg caatggcatt cctgtgcggc tcgccgccga
     4081 agtgctccgc gctttctacc actacgactg gcccgggaac gtgcgggagc tggtcaacga
     4141 actctcgcgc attcttgttt tggccaaagg ggtgccggtc atcggagcgg agtacctctc
     4201 cgacaaattt acttccgcag ccgcctcccc gcttttgccg gggtctctgg aaacgcgcct
     4261 ctcccagtac gagcgcgacc tgatcgaaaa ggcgctctcc gcctccgacc gggtgaaagc
     4321 ccaggccgcc cgcattctgg gcatccccgt caccaccctg cgctacaaga tgggcaagca
     4381 cgggctgaac tggaagtagt tcttcgttca aaagcataat tccaccacag aggcacggag
     4441 ggcacggaga aaatcgtgaa ctctccctcc ataaaatccg tttaatctcg tagagtcgta
     4501 ccgaccgctc caaaatccgt tgtcccatac gggtcgtacc gacagaattt ggggttacga
     4561 caggcgtaaa acggtctctt ttctcctccg taggcggggc gtctcgcccc gcttaattcg
     4621 gggtcatcgc ggaggtaaaa cggacaaagt gcgaaatagt agagggtcgc tagctcgacc
     4681 ctcagagact ggtgccgaga accggcctct gcggactggc ctccttcaag ccgcgcgcgt
     4741 taaacaaaga gcttcaagcg ccagtgcacg ccggtcggtc cgataaagta ggatcgggca
     4801 tcacttctcg tcagggagaa aaaggccccg gggccgccct ggaaaaaaca gggcaaaaga
     4861 aagcaaaaga agatgaaagt gaaagggatt agggcaaaaa tgaattaact taccagttcc
     4921 tgccagaccc ggtggcggag ttcttctttc aggcgtgggc tcaaaaggcc gtcggcgtgt
     4981 tcttcgataa tttcgtctat gtttcttttc agccgctcca gtttgggcaa tatttctttt
     5041 aaaattctgt catctttggg cactttcact tcttgcatcc cggcatccaa aaagaccttc
     5101 aaaaaccggg cctcctcttc cgagcggcaa tcgatgaact ccttcccgga gtagagcttc
     5161 cagccaaaaa ggtctttgac gatttcgctt ttatcccttt gcgtcggcag ttgcctcttg
     5221 gacagcggct tgtgcgggag cacttcttcg cggtagaatt cccctatcgt tctcattttt
     5281 ccttttttcc cttaacctgt tttaccacgc cgcccgccag cgcgtccaca tccacacctt
     5341 caatatatct tttacccttc tccacgctct gggccttttc gattctcgac cggaccaaat
     5401 ctaccactgc cttataaact tccaactgct cttcttcctt cagccctaat atgtcccgca
     5461 ttattatttt atccaactcg cgccggtcag gcttggcggt attaaaagag acattacctc
     5521 cagaaaatac tcctaattcg tctaaaatat gtgaaatcgg acgaacgcta agcttgcgga
     5581 acgctcgcag aatatccagc ctagtgctac ttggtatttg ggctgtatca attataggaa
     5641 cctgactcaa caaccccatc tcaactccaa ttacattaat agctcctgtt agattacggc
     5701 catgcaagtc atggcataat tgaaaaacaa tagaattcat tattgcgcat aaaacttctg
     5761 cttcatcatc ttccttgtag agtggataaa cttcatgaag attctgatta gttagaacgc
     5821 cctccctatt gaacagtatt aagaatctat ctcccatgta caaaggatga agtataggtg
     5881 cagggtctct ttcgcccagc gaataccata gtggtcttga agagcaagtc ttcgttttgg
     5941 ccggaatgta gctccgtttc ccctttgaaa atgttctgtt ttcaccctgt cgtatatact
     6001 gcaagatact tttaggaagc tttgactttt cctcatttgc ccaaaaaaca cttttgggga
     6061 gtttagcgac ccgaatgaca atggattcag tttcatccag tgaaacaata ataggcttaa
     6121 catatttggg ggtcagctgc aaatgcaata aatcttcttt tgaaagataa aaaaaatcat
     6181 cggctccact ctttatgcca aactttatat tagcaatttg acgaaaagga acaatctttc
     6241 ctttcgcttt ttccaaaatc tcaaagaaga tctctggagc gcgtagatat ctgccccatt
     6301 tggaaccaac ataattttcc ttcttttctt taccggtatg ctcctggccg ccgtcttctt
     6361 ctatttccac cccttcttcc cagagttctt tctgactttt ggggaaaatt cgtaattcct
     6421 cattttcata aagagaatca tgagacagaa tagtgttgat caggttatca atggcgcgca
     6481 gtcggctcag ttcttgcgcc cagatgtcac ctgcaggggg aatgaagtgg gtaagtggtt
     6541 tcttgaggta tacaaagcgg cataagtttg catcccgctc gatcttttta tcacat
//
LOCUS       DBNGZA010000210         5074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_675784, whole genome shotgun sequence.
ACCESSION   DBNGZA010000210 DBNGZA010000000
VERSION     DBNGZA010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_675784"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16FU_04355"
     CDS             <1..506
                     /locus_tag="AC16FU_04355"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2993638.1"
                     /translation="LRLRDFVNWGADPTRLVGVDITPERIAQARRLSPNITFVCANAD
                     RLEFSNGSFDLVFQFSTFTSLLDYAMKRRVAEEMLRVLANDGCILWYDFRVRGREREH
                     SAPIGRREIRELFAEGTVRLRSMTLAPPLARRLAPYWWTGCLLLEKIPLLRTHWFGVI
                     RKKRPTA"
     gene            503..1708
                     /locus_tag="AC16FU_04360"
     CDS             503..1708
                     /locus_tag="AC16FU_04360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR2993639.1"
                     /translation="MTRSKIVSDKQPEARQIPFYQPSITEREIRAVVQTLRSGWLTTG
                     PKTKKFEKRFARYVGAKHAVALNSCTAGLHLALVASGVGPGDEVIVPTYTFAASAETV
                     IHAGAKPVLVDSESDTLNLSVAEVEKEISPRTKAVVAVDIAGLPCDYAALQPVVKKQG
                     IHLIDDAAHALGASYDGRMVGTLADLTAFSFYATKNLTTGEGGMVTTDNQELAEKISL
                     LSLHGMSRDAWKRYRTGGSWYYELLDAGFKYNFTDLQAALGLPQLARLRTLQAQRRAL
                     ATLYDRELGDLPEVRLPAQKKGRVHAWHLYIIRLRLEQLKIDRNQFIHELTRRRIGTA
                     VHFIPLHLHPYYRERYGYQPEDFPVAHDNYQRAITLPLYPRMTRAMVCYVAGAVREIV
                     QKYRHRGRG"
     gene            1705..4116
                     /locus_tag="AC16FU_04365"
     CDS             1705..4116
                     /locus_tag="AC16FU_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR2993640.1"
                     /translation="MSVTPPVIAKKLRNTVLLFLGDQAVLWGAFLFALWLKGLTRNLG
                     FFDYLEPGLLLAGLWVVIFLVSGIYWRGSRSFLHELLRINNAVALGILILLLATFDVT
                     SPLSQVRVTLLGYWAVLLAGLWLFRWLFWQKFNRQLVSGFPELPTPLVLLRRLGILGM
                     DLALAGLSLFLAFLLRFDGRIPANHLTIFFYLLPLVVIIRFSAFSLAGLYSGLNRYAS
                     IADLFTVLKGVSFGTALFVLPAAVQWLVNHLDPKVASTLGLTWLLPLFPRLHGYPRSV
                     FFIDWLLMVIFIGGSRFAIRALRELAPNFLREGKRTLIVGAGNAGEMLMREIRRNRSL
                     PYLPVGFIDDDPNKRGLRVHGLPVVGSHREIPQLVSRLGAEQIVIAIPSATGIQMRRI
                     VEACQQSKVEIQTLPPLKEILGGQVDLHQVRKVRLEDLLGREPVQIETGQIASFLEGR
                     RVLITGAAGSIGAELVRQVLRFRPLQVLAVDRAENNVFALEQEIAARTDRSRAMLSIG
                     DVTDGIRMDDLFASFRPEVVFHAAAYKHVPLMEDHPDEAVKNNVAGTIGMADLAERYG
                     TRKFVFISTDKAVHPTSVMGATKRVAELYLQAKPAAPTAFVSVRFGNVLGSDGSVVPL
                     FQKQIARGGPVTVTHPEVSRFFMTTSEAVQLVLQAASIGQAGSVLLLDMGEPIKILDL
                     ACDLITLSGYVPHAEIPIVFTGLRKGEKLHEELVVADEGVTPTSHPKILVARAPRPAD
                     NGFSLNLSNLVACAQRGERQQVKVLLADLVGTYRPNGEGESADRSADSPPGEALAADA
                     VQGSR"
     gene            4137..>5074
                     /gene="serS"
                     /locus_tag="AC16FU_04370"
     CDS             4137..>5074
                     /gene="serS"
                     /locus_tag="AC16FU_04370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR2993641.1"
                     /translation="MLDLKFIRERLEQVKDGVRHKNHQVDIDQLLSLDEERRRLIARS
                     EELKRKRNETTEEIALLKKEKKDAEKLITEMRQVGQEIAILDEQLKQLEQEVETLLWR
                     VPNLPHASVPVGKDETANVEVRRWGKLPEFPFTPRPHWELGQALGILDLPRAAKISGS
                     GFITFTGIGARLERGLISFMLDLHTQKHGYTEVLPPFLVTTAAMYGTGQLPLLAQDMY
                     RLEQDDLWLVPTAEVPVTNLFRDEILEALDLPIYRVAYTPCFRREAGSYGKETRGIIR
                     VHQFDKVELVKIVRPEHSYAELEKLVTDAEEVLQLLG"
BASE COUNT          981 a         1503 c         1513 g         1077 t
ORIGIN      
        1 gtttgcgact tcgcgatttc gtcaactggg gtgccgaccc aactcggctc gtcggcgtgg
       61 acatcactcc cgagcgaatc gcgcaggccc ggcgcctttc cccgaacata accttcgtat
      121 gcgccaacgc agaccgactg gaattttcca acggctcttt tgacttggtc tttcagtttt
      181 ctactttcac gtcacttttg gattatgcca tgaaacgacg ggtagcggag gaaatgttgc
      241 gggttttggc gaacgacggt tgtatccttt ggtacgactt tcgggtgcgg ggtcgagagc
      301 gggagcattc cgcccccatc ggtcgccggg aaattcgtga actgtttgcg gagggtacag
      361 ttcggctgcg gtcgatgact ttagcaccgc cgttggctcg gcgattggcg ccctactggt
      421 ggaccgggtg tcttcttctg gaaaaaattc cgctcctgcg tacccattgg tttggagtga
      481 tccgtaaaaa aaggccgacg gcatgacccg gtcaaagatt gtgtcagaca agcagcctga
      541 agcacgccag ataccattct atcaaccctc catcaccgag cgagaaatca gggcagtggt
      601 gcaaaccctg cgttccggtt ggctcaccac cgggccgaaa accaaaaaat ttgaaaaaag
      661 atttgcgaga tatgtcggcg ccaaacacgc cgtcgcgctc aactcctgta ccgccggctt
      721 gcacctggct ctggtggcct ccggcgttgg gccgggggat gaagtcattg tgcccaccta
      781 cacttttgca gcttcagccg aaacggtcat ccacgcgggc gccaagcccg tcttggtcga
      841 ttccgaatcg gatacgctca acctttccgt ggcggaggtc gaaaaggaaa tctcccccag
      901 gaccaaggcg gtggtcgcgg tggatatcgc tggcctaccc tgtgattacg cagcgttgca
      961 gccagtggtc aagaagcagg gcatacatct gattgatgac gccgctcatg ccctgggggc
     1021 atcatatgat gggcggatgg tcgggaccct ggctgatctg accgccttca gtttttacgc
     1081 cacgaagaac cttaccacgg gtgagggggg catggtcacc acggacaacc aggaactggc
     1141 ggaaaaaatt tctcttttaa gtctgcacgg gatgtcgcga gatgcttgga aacgctaccg
     1201 taccggcggg tcgtggtact acgagcttct ggatgccgga ttcaaataca acttcaccga
     1261 tctccaggcg gccctgggat tgccccaatt ggcccgcctg cgtaccttac aggcacagcg
     1321 ccgggcgctg gcgacgctct atgatcggga attaggagat ctgcccgaag tgaggcttcc
     1381 ggcgcaaaaa aaaggacggg tacacgcctg gcacttgtat atcattcggt tgcggctgga
     1441 gcagcttaaa atcgaccgga atcaattcat ccatgagtta acccggcgca ggatcggtac
     1501 cgctgtgcac ttcatcccgc tgcacctcca cccgtactac cgggagcggt acggctacca
     1561 gccggaagat tttccggtgg cacatgacaa ctaccagcgc gccattacgc tgccgttgta
     1621 tcccaggatg acgcgagcca tggtgtgcta cgtcgccggc gcagtgcgtg aaatcgtgca
     1681 gaagtaccga catcggggga gagggtgagc gtcacgccgc ccgttatcgc caaaaagctc
     1741 cgcaataccg tgctcctgtt tctgggcgat caggcggtcc tgtggggagc tttcctgttc
     1801 gccctctggc tgaaaggact cacgaggaat ctcgggtttt tcgattacct tgagccgggt
     1861 ttgctcctgg ccggcctctg ggtcgttatt tttctcgtct cgggaatcta ctggcgcggc
     1921 tcacgatcct ttctccatga acttctgcgc attaacaatg ccgtggcgct gggtattctc
     1981 attctccttt tagccacgtt cgacgtcacc agccccctct cccaggtgcg ggtgaccctg
     2041 ctcggctact gggcggtgct cctggcgggg ctttggcttt tccgctggct cttctggcag
     2101 aaattcaatc gtcaattggt ctccgggttt cccgaactgc cgaccccttt ggtcctcctg
     2161 cgccgtctcg gcattctggg gatggatttg gccttggccg gcctctcgct tttcctcgcc
     2221 tttcttttgc gctttgacgg gcgcattccc gccaaccatc tgaccatttt tttctacctt
     2281 ttgccgctgg tggtgattat ccggttttcg gctttttctc tggcgggact gtacagcggc
     2341 ctgaaccgct acgccagcat tgccgatttg ttcacggtgc tcaaaggagt cagtttcggc
     2401 actgccctgt ttgttttgcc ggccgcggtg cagtggctgg tgaaccacct cgatccgaaa
     2461 gtggcctcaa ccttggggct gacctggttg ctcccgttgt tccccaggct gcacgggtac
     2521 ccgcggtcag tctttttcat cgactggctg ttgatggtga tcttcatcgg tgggtcgcgc
     2581 tttgccatcc gggcgctgcg ggagctggcg ccgaattttc tgcgcgaagg caaacgcacg
     2641 ttgatcgtgg gggcgggaaa cgccggggag atgcttatgc gcgaaattcg gcgcaaccgt
     2701 tcgctcccct acctgcccgt cggcttcatc gacgacgacc cgaacaagcg cggcctgcgg
     2761 gttcacgggt tgccggtggt cggcagccac cgcgaaatcc cgcagttggt atcccgcctg
     2821 ggagccgaac aaatcgtcat tgccattccc tcggccaccg ggatccaaat gcgccggatc
     2881 gtcgaggcct gtcagcagag caaagtcgag atccaaacgt tgccgcccct gaaggaaatt
     2941 ctgggggggc aggtggacct gcaccaggtg cggaaagtgc gtctggaaga cctcctgggc
     3001 cgcgaaccgg tgcagattga gaccggacaa attgccagtt tcctggaagg ccgccgggtc
     3061 ttgatcaccg gcgcggcggg ctccatcggc gccgaactgg tgcggcaagt tttacgcttc
     3121 cgtccccttc aggtgctggc ggttgaccgg gccgagaaca atgtctttgc gctggagcag
     3181 gaaatcgccg cccgcaccga ccgctcccgg gccatgctct ctatcgggga tgtgaccgac
     3241 ggcatccgga tggacgacct gttcgcttct tttcggccgg aggtggtgtt ccacgccgcg
     3301 gcctacaagc acgtgcccct gatggaagat caccccgacg aagcggtgaa aaataacgtg
     3361 gccgggacga tcggcatggc cgacctggcc gaacgctacg gcactcgaaa attcgttttc
     3421 atttccaccg acaaggcggt ccaccccacg agcgtgatgg gggcaaccaa gcgggtggcg
     3481 gaactgtacc tgcaggctaa gccggctgca cccactgcgt tcgtctccgt ccgcttcggc
     3541 aacgttctgg gctccgacgg gagcgtggtg ccgctcttcc agaaacaaat cgcgagaggc
     3601 gggccggtga ccgtcaccca tccggaggtg agccgctttt tcatgaccac gagcgaagca
     3661 gtgcagttgg tcttgcaggc cgcctccatc gggcaggccg gcagcgtgct gcttctggac
     3721 atgggggagc cgatcaaaat tctcgacttg gcctgcgacc tgatcaccct ctccggctac
     3781 gtcccccatg cggagatccc catcgttttc accgggctgc gcaaagggga gaagctgcac
     3841 gaagaattgg tggtggccga cgaaggcgta acccccacca gccacccgaa gattctggtg
     3901 gcccgggctc cccggccggc agacaacggc ttttctctca atctttccaa tctcgtggcc
     3961 tgcgcccaac ggggggagcg gcagcaggtc aaagtactcc tggccgattt agtcggcact
     4021 taccggccga atggggaagg agaatcggcc gaccgcagcg cagacagtcc ccccggcgag
     4081 gcactggcgg ccgacgcagt ccagggttct cggtaaacaa attttctcat cccgtcgtgc
     4141 tggatctcaa attcatccgc gagcgcctcg aacaggtcaa agacggggtg cgccacaaaa
     4201 atcatcaggt cgatatcgat cagctgcttt ctttagacga agaacggcgg cggctgatcg
     4261 cccgctccga ggaattgaag cgcaagcgca atgagacgac cgaagagatt gcacttctga
     4321 aaaaagagaa aaaagatgcc gaaaaattga ttaccgaaat gcgccaggtg ggacaggaga
     4381 tagccatcct ggatgaacag ttgaagcaat tggaacagga agtcgagacg ctcctctggc
     4441 gggtgccgaa tctcccccat gcctcggtgc cggtggggaa agacgagacg gccaacgtgg
     4501 aggtgcggcg gtggggaaag ctcccggaat tcccctttac cccgcggccc cactgggagc
     4561 tgggtcaggc attgggcatt ctcgatctgc cccgggcggc gaaaatctcc ggttcgggat
     4621 tcatcacctt caccggtatc ggcgcaaggc tggaacgggg actgatttcg ttcatgctcg
     4681 atctccacac gcagaagcac ggctacacgg aagtgctgcc gcccttccta gtcaccaccg
     4741 ccgcaatgta cggcacgggt cagttgccgc ttctggcgca ggatatgtac cgcctggagc
     4801 aggacgacct ctggctggta cccacggcgg aggtgccggt gacgaatctg ttccgcgatg
     4861 aaatcctgga agcccttgac ctccccatct accgggtagc ctacaccccc tgcttccggc
     4921 gggaggctgg ctcctacggc aaggaaacgc gcggcattat ccgggtacat cagttcgaca
     4981 aagtggaatt ggtcaaaatt gtccgtccgg agcattcgta tgctgaactg gaaaaattag
     5041 tgaccgacgc tgaggaagtt ctccagttgc tcgg
//
LOCUS       DBNGZA010000211         3454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1630628, whole genome shotgun sequence.
ACCESSION   DBNGZA010000211 DBNGZA010000000
VERSION     DBNGZA010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1630628"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="ytpR"
                     /locus_tag="AC16FU_04375"
     CDS             complement(<1..434)
                     /gene="ytpR"
                     /locus_tag="AC16FU_04375"
                     /inference="COORDINATES: protein motif:HMM:NF045760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HIR2993642.1"
                     /translation="MKVSYNWLRELIDFPWDPKELAEKLTLAGLEVGSVTPQYPNLDG
                     VVVGRIESVERHPQDEHLFYCQVNIGRERLAIVSGAPNTVSGVKVPVAPPDTVLPDGT
                     LVQKKTIRGVESFGMVCSERELGLSDDHSGLMILDGDSEIGQP"
     gene            complement(536..1555)
                     /gene="pheS"
                     /locus_tag="AC16FU_04380"
     CDS             complement(536..1555)
                     /gene="pheS"
                     /locus_tag="AC16FU_04380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR2993643.1"
                     /translation="MSDRSEEIESIRAEAERAIAAAPDENDLDQVRVTYLGRKGRLTA
                     ILRNLKEVPEGRRPQVGQAANAVKTFLEGLIAQKETELKTSARPAAPAFDYTLPGRGH
                     AVGKLHLVTQTTDAIVRIFSRMGFDTAYGPEVELDYYNFAALNFPPEHPARDMQDTFY
                     VSEDVVLRTHTSPVQVRAMEKRQPPLKILSPGRVYRHESINVRSHVLFHQVEGFAVDK
                     DITFGDMEGVLSVLAWELFGRDVGIRFRPSFFPFTEPSAEVDISCTICGGKGCGVCKN
                     EGWVEIAGCGMIDPEVFRSVGYDSELYTGYAFGLGVERVAQLKHRIDDVRLFLENDLR
                     FLRQF"
     gene            complement(1638..1988)
                     /gene="rplT"
                     /locus_tag="AC16FU_04385"
     CDS             complement(1638..1988)
                     /gene="rplT"
                     /locus_tag="AC16FU_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR2993644.1"
                     /translation="MPRATNNPAARRRKKKILKQARGYFGGRSKLYRTAREAVQRGLA
                     YAYRDRRARKRDFRRLWIVRINAAARLYGLTYSLFINGLHKAGVALDRKILADLAVRD
                     QATFAKLAELAKSA"
     gene            complement(2001..2195)
                     /gene="rpmI"
                     /locus_tag="AC16FU_04390"
     CDS             complement(2001..2195)
                     /gene="rpmI"
                     /locus_tag="AC16FU_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR2993645.1"
                     /translation="MPKLKTNRSAAKRFRKTAKGRFKHSQAGRRHILSSKSAKRKRQL
                     RGAAYVEPVDEKKLKRLLPY"
     gene            complement(2206..2841)
                     /gene="infC"
                     /locus_tag="AC16FU_04395"
     CDS             complement(2206..2841)
                     /gene="infC"
                     /locus_tag="AC16FU_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR2993646.1"
                     /translation="MKGERELRVNYRIRIPQVRVIDYDGKQLGVMSTRDAQRLANDRG
                     LDLVEVAPTVRPPVCRIMDYGRYKYEQNKKARTTKKKQHVTQVKEMHFRPKTDEHDYR
                     FKLKHVREFLEEGDKVKVSVDFRGREMAHQEFGHKLVERLVKDVADTGQVEQRAKLEG
                     RNLILLLAPFHRRAHSPARATEGTESPLRPQTSRTSGTTHAADEPIPTEGT"
     gene            complement(2838..>3454)
                     /locus_tag="AC16FU_04400"
     CDS             complement(2838..>3454)
                     /locus_tag="AC16FU_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR2993647.1"
                     /translation="LPYVVAPGEANFYGPKLDVQIKTATGQEETLSTVQLDFHLPERF
                     GLEYAGADGQPHRPVMIHRAIISTLERMTAFLIERYVGAFPLWLAPVQVAVLPITDAQ
                     VDYARFIHERLVGEGFRAVLDLGNEKIGYKIREAEVQKVPYMFIVGKKETEAGTVALR
                     RHGQGDLGAKVLESAIAVLQAEVETKAVGPPGEKLEVSKRRETP"
BASE COUNT          617 a         1052 c         1039 g          746 t
ORIGIN      
        1 ggctggccga tttcggagtc accgtccaga atcatcaaac cggaatgatc gtcggagagt
       61 cccagctccc gctcggagca gaccatcccg aaagattcga ctcctcggat cgtttttttc
      121 tgcacgagag tgccgtcggg aagcaccgta tcgggtggtg ccacgggcac tttcaccccg
      181 gaaaccgtat tgggcgcgcc ggagacaatg gcaagtcttt cgcggccgat gttgacctgg
      241 cagtaaaaga gatgctcgtc ctggggatga cgctcgaccg actcgattcg cccgaccacc
      301 actccatcta agttagggta ctgcggcgtg accgacccca cctccaggcc ggccagggtc
      361 aacttctcgg ctaattcttt cgggtcccag gggaagtcaa tgagttcgcg gagccagttg
      421 taggaaactt tcatgattta tcgggtatat tcttgaagtg gtaacggttt acccggggat
      481 tcccgcactt tcctttttgg tgctttcggg ggacgagggc agcgaatcgg tggcattaga
      541 attgccgcag aaagcgcaaa tcattttcta agaagagccg cacgtcatcg atccggtgct
      601 tgagttgagc aacccgctcc acccccagac cgaaggcgta gccggtgtag agttcgctgt
      661 catacccgac ggagcggaaa acttccgggt cgatcatccc acagccggca atttccaccc
      721 agccctcatt tttgcagacc ccgcagcctt tgccgccgca gatggtgcag gaaatatcaa
      781 cttcagccga gggctcggtg aaagggaaaa acgagggtcg gaaacggata ccgacatccc
      841 gaccgaagag ttcccaggcc aggaccgaaa ggactccctc catgtcgccg aacgtgatgt
      901 ctttgtccac cgcaaaacct tccacctgat ggaacagaac gtgggagcgg acgttaatcg
      961 actcgtgccg gtaaacccgg ccgggcgaga gaattttcaa aggcggctgc cgcttttcca
     1021 tggcccgcac ctgcaccggc gaggtgtggg tacgcagcac cacgtcctcg gaaacgtaga
     1081 aagtatcctg catatcccgg gccgggtgct ccgggggaaa gttcagggct gcgaaattgt
     1141 agtagtccag ttccacttcg ggcccgtagg cggtgtcgaa acccattcgg gagaagatgc
     1201 gcacgatggc gtcggtggtc tgggtcacca ggtggagttt cccgaccgcg tggccgcggc
     1261 cagggagcgt gtagtcgaaa gcgggcgccg ctggccgagc gctcgttttc aactccgttt
     1321 ctttttgcgc gatgagccct tcgagaaagg ttttcaccgc attggccgcc tgtcccacct
     1381 gagggcgccg gccttcggga acttctttga ggttgcggag gatggccgtc aagcgcccct
     1441 tgcggccgag gtaggtcacc cgcacctggt ccaggtcatt ctcatccgga gcggccgcaa
     1501 tcgcccgctc tgcctcggcc cggattgatt cgatctcttc ggaacggtca ctcatgactt
     1561 acccaaatga caaagggcac aggccaatcc gcctgaggcg gattggcctg tgccccaaac
     1621 gtcagccacg attccctcta agcactcttg gccaattcgg ccagtttggc aaaggtcgcc
     1681 tggtcgcgga cggctaagtc ggccaaaatc ttccggtcga gggcgacacc ggctttgtgc
     1741 aaaccgttga tgaaaaggct gtaggtcaaa ccgtacagcc tggcggcggc gttgatccgc
     1801 acaatccaga gccggcggaa gtcacgcttg cgggcgcgcc ggtcgcggta agcgtaggcc
     1861 aggccgcgct gcaccgcttc gcgggcggta cggtacaact tgctgcggcc gccaaagtag
     1921 ccccgggcct gcttgagtat cttcttcttc cggcgtcggg ccgccgggtt gttcgtagcg
     1981 cgtggcatcg aatggtcctc tcagtacggt aataaacgct tcaatttctt ttcgtcaacc
     2041 ggctccacgt aagccgcccc gcgcagctga cgtttccgct tggcagactt ggaagagagg
     2101 atgtgccggc gtccggcctg gctgtgctta aaacggccct tggcggtttt ccgaaagcgc
     2161 ttggcggcgc tccggttcgt cttgagtttc ggcatagttt tccccttagg taccctcggt
     2221 gggtatcggt tcgtccgcgg cgtgagtcgt cccggacgta cggctcgtct gaggtcgcag
     2281 cggtgattcg gttccctctg tggcccgggc tgggctgtgc gcccgccggt ggaacggcgc
     2341 caagagcaaa atcaggttgc gtccttccag tttggcgcgc tgctccacct ggccggtgtc
     2401 ggccacgtcc ttcaccaaac gttccaccag cttgtggccg aactcctggt gggccatttc
     2461 ccggccgcgg aagtcgacgg aaactttgac cttatccccc tcttccagaa actcccgcac
     2521 gtgcttcagc ttgaagcggt agtcgtgctc gtcggtcttg ggtcggaagt gcatttcctt
     2581 cacctgcgtc acgtgctgct tctttttggt ggtccgggcc ttcttgttct gttcgtactt
     2641 gtaccggccg taatccataa tgcggcagac cggcggccgc acggtgggcg ccacctccac
     2701 taaatccagt ccccgatcat tggccagccg ctgcgcgtcg cgcgtgctca tcacccccag
     2761 ctgcttgccg tcgtagtcga tgacgcgcac ctggggaatc cgaatccggt agttcacccg
     2821 cagttcccgc tctcctttta tggtgtttcc ctccttttgg acacttccaa cttttccccc
     2881 ggtggtccga ccgctttggt ctctacctcg gcctggagga ctgcgatcgc ggactccaac
     2941 actttcgcgc ctaagtcacc ctggccgtgg cgccgcaggg cgacggtccc ggcttcagtt
     3001 tcttttttcc ccacgatgaa catgtacgga actttctgga cttccgcctc ccggatttta
     3061 tagccgattt tctcgttgcc gagatcgaga acggcgcgga atccctcacc gacgagccgc
     3121 tcgtgaatga agcgggcgta gtccacctgc gcgtcggtaa tgggcagaac ggccacctgc
     3181 accggcgcca gccagagggg gaatgccccg acgtagcgtt cgatcaaaaa ggcggtcatc
     3241 cgctccaggg tgctgatgat ggcccggtga atcattaccg gtcggtgggg ctggccatcg
     3301 gcgccggcgt actccaggcc aaagcgttcc ggcaggtgga agtcgagctg gacggtggag
     3361 agggtttcct cctggccggt ggcggttttg atctggacgt ctaatttcgg tccgtagaag
     3421 ttggcctccc cgggggcaac gacgtagggg agtc
//
LOCUS       DBNGZA010000212         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2690194, whole genome shotgun sequence.
ACCESSION   DBNGZA010000212 DBNGZA010000000
VERSION     DBNGZA010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2690194"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="AC16FU_04405"
     CDS             complement(<1..1288)
                     /locus_tag="AC16FU_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993648.1"
                     /translation="MVSIQKKIWWLSSAFLFSLISHHEVCAEALRLNAKVIWTQADRV
                     YCVSSDSGAFEPAKNLTFVYRGREVARGKIVALYNRELALVQLQWGSLINIDHLDSVE
                     VAEQPAPLPPLSRLRIGYPSQKRSNLIFSCSEMAFDHSSIPAAFRPDTLEDAGDQWVR
                     DSLKNPKPDPWPDTLFVREVDEVSDEEIALERGELDIAVFWPGELSQHMRKDPRWQNP
                     AFGRAQQGLVAALWLSQKIDEDDSNLCDLRRWYDFDLFNEELFRGDLVPVEQAAAWAA
                     QSPAVQPHPPAPVVRFQVERSWPGQRLVERFLNRRSKSSPAVTTPGQMETVRLFYLDA
                     PVSSFDSISQALAAYLQNGDFPQEIRARADTLLTLVRAAHNAPDSIRVSALDTVQHYV
                     QNRLHVTLIFCPRCPVICKPELRAYLEALGSDMLVNL"
     gene            1565..2158
                     /locus_tag="AC16FU_04410"
     CDS             1565..2158
                     /locus_tag="AC16FU_04410"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR2993649.1"
                     /translation="MSKRKNLAIAIAISLSASAVNARPGDSLHVIINIPAFHLFLFSG
                     DSLLASYPIAVGKPETQTPVGTFTVKRKVMNPSYWPKGQPPVPPGPGNPLGRYWIGLS
                     QRDLGIHGTNEPHSIGTAASGGCIRMRESDLLALYDRVLLGTRVEIVYETRREGGGRQ
                     SAYADIYHHNLNNSKRNTTEPEPETLPLLAQGNSGKR"
     gene            2233..2820
                     /locus_tag="AC16FU_04415"
     CDS             2233..2820
                     /locus_tag="AC16FU_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993650.1"
                     /translation="MKTTQIKQDTAAHPLRLWRKLGLASLALAVTFLASACSERGNKD
                     EARKEASVKSAEAAEPMTPNAAAEENDSLGLPRAQEQEGTVVTADSLLPDVSAFAADT
                     LTYPGGTVEITARTSSDVTAVALWDGIGQKQAFTCDSEAKVWRITYRVPLKPLWERLG
                     LSVTARNSAKKWRRVWVFLQLQGQAPQAQAAPVEK"
BASE COUNT          699 a          860 c          810 g          565 t
ORIGIN      
        1 ccagattcac aagcatgtcg ctacccagcg cctccaggta ggcccggagt tccggcttgc
       61 agatgaccgg acagcggggg caaaagataa gcgtgacgtg caaccgattt tgcacgtagt
      121 gctggaccgt atccagcgcc gacactctga tgctatcggg agcgttgtgg gcggctctaa
      181 ccaaagtcag cagtgtgtcc gcccgggcgc ggatttcttg cggaaagtcg ccgttctgaa
      241 ggtacgccgc cagtgcctga gagatggaat cgaaagaaga aaccggagcg tcgagataaa
      301 agaggcgcac cgtttccatc tgaccgggag tagtcacggc agggctcgac ttgctccgac
      361 ggttcagaaa gcgctccacc agccgctgtc ccggccaact gcgctcgact tgaaagcgca
      421 caaccggggc cggtgggtgc ggctgtaccg cgggagactg agccgcccag gcggccgcct
      481 gttcaacggg cacgaggtcg ccccgaaaca gttcctcgtt gaaaaggtca aaatcgtacc
      541 agcgtcgcag gtcgcagaga ttggagtcgt cttcatcaat tttctgactc agccagaggg
      601 cggcgaccag cccctgctgc gcgcgcccaa acgcggggtt ctgccagcgg ggatctttcc
      661 gcatatgctg cgagagttcg cccggccaga agacggcgat atccagttca ccccgctcca
      721 gggcaatttc ctcatcagag acttcgtcaa cctcccggac gaaaagggta tccggccagg
      781 gatcgggctt tggatttttc agcgaatcgc gcacccattg gtcgcctgca tcctccagcg
      841 tgtccggacg gaaggcagcg ggaatgcttg aatgatcaaa tgccatctca gaacaagaaa
      901 aaatcagatt agaccgtttt tgagaaggat acccgatccg caggcgggat aaaggcggga
      961 ggggtgccgg ctgctctgcg acctccactg aatccaggtg gtcaatatta attaacgagc
     1021 cccattggag ttggaccagc gccagttcgc ggttgtacaa cgccacgatt ttcccccggg
     1081 caacttccct gccgcggtac acgaacgtaa gatttttggc tggctcgaag gcaccggaat
     1141 cggaggaaac acagtagacg cggtcggcct gcgtccaaat cactttggca ttcagccgca
     1201 aggcttccgc acaaacctcg tggtgggaaa taagcgaaaa aagaaaggcc gaacttaacc
     1261 accaaatttt tttctgaatt gagaccatac cctcaacata atccttactg ctctcgaact
     1321 tcaaagttcc ctattctgtg tcccaaaaat acttatacgt gtcaaatttg ccacgaacga
     1381 ctaactaatt gtagtcatta aactttccgt taccatccta acagttaaga ccatgtgcgt
     1441 gttcgttttg acacatattt tcttctcttt ttgaccgcct catcagcata acctgttaaa
     1501 cgacaaccaa ataagtagac ggcattgatt ttgacatcat ttgctcccgg agatgaagat
     1561 gatgatgtca aaacgaaaaa acctcgcaat cgccatcgca atcagtttat cggcctctgc
     1621 ggtaaacgct cgacccggcg attcactgca tgtcatcatc aatattcctg cgttccatct
     1681 ctttctcttt agcggtgata gtcttctggc ttcttacccg atagcggtcg gcaaaccgga
     1741 gacgcagacg cctgtcggca cgttcaccgt aaagcgcaag gtgatgaatc ccagttactg
     1801 gcccaagggc cagccgccgg ttcctccggg gccgggtaat cctctcggcc ggtactggat
     1861 cggcctctcg caacgcgacc tgggaattca cgggacgaac gaaccgcact ccatcggcac
     1921 cgccgcctcc ggcggttgca tacggatgcg ggaaagcgac ctcctggcat tgtacgaccg
     1981 ggttctcctt ggcacccggg tggagatcgt ctatgaaacc cggcgagaag ggggcggccg
     2041 gcaaagcgcg tatgcggaca tctaccacca caacctaaac aatagcaaac gcaacactac
     2101 cgagcctgaa cctgagactc tgccgctctt ggcacaggga aattcaggga agcgttaact
     2161 gatcgattgc cagtaaccag tacctgacgg tcaatcagta aaaaatcatc aaaaaccgga
     2221 aaggagagac agatgaaaac cacccaaatc aagcaggaca ccgccgctca cccgctgcgc
     2281 ctctggcgca agctggggct ggcctcgctc gctctggccg tgactttcct cgcttccgcc
     2341 tgcagtgagc gagggaacaa ggacgaggcc cggaaagaag caagtgtaaa atcggcagaa
     2401 gcggcggaac cgatgacccc gaacgccgcc gcagaagaga acgactcgct cggtttgccg
     2461 cgggcccagg agcaagaagg aactgtggta accgccgatt ctctcctgcc cgacgtttcg
     2521 gctttcgccg ccgacacgct gacgtacccc ggcggcacgg tggaaattac cgcccggact
     2581 tcctccgacg tgaccgcggt cgccctctgg gatggcatcg ggcaaaaaca ggccttcacc
     2641 tgcgattccg aggcgaaggt ctggcggatc acctaccggg tgcccctgaa accgctctgg
     2701 gagcgcctgg ggctgtcggt gaccgccagg aacagcgcca aaaagtggcg ccgggtctgg
     2761 gtcttcctgc agcttcaagg gcaagcgccg caagcccaag ccgcgccggt ggaaaagtaa
     2821 ccgccgagcc ctgtagttaa gtggtaagac gaaagcgccg caggtcaaag ctcccgtgag
     2881 caggtgatct gcggcgtttt ttatcatcca ggaaaaaccg gggttaccaa tcaa
//
LOCUS       DBNGZA010000213         5338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2902913, whole genome shotgun sequence.
ACCESSION   DBNGZA010000213 DBNGZA010000000
VERSION     DBNGZA010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2902913"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="AC16FU_04420"
     CDS             <1..1478
                     /locus_tag="AC16FU_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR2993651.1"
                     /translation="HWKGKILEIKPSLFWEGERTYTFTFGGQAQDGHGIRMGETFTFS
                     FSTNTVIDSGRIEGLVFAGLVPEVGASVCAYRLNSTADIDPMRQKPEYVTVSGSSGKF
                     RLSALADGRYRLFAVKDQNRDRLWQPKREPFAVGTGEITLDSNSRHQNGLQFFLAEQD
                     TAAFGLVRCQNLGPFVVWLEFNQPLDFGAWNWQENFELRERSDSAGRRSDVTKITRAA
                     GMYEFDAEPKNLYLVTERLSIGSTYDLILKSFTDRYGKPLDSARAHCRFTVAAEPAPP
                     LRIVQTIPRSGEKYFDPGQLPLLVVNYPLKSPVDDAAVLLQDTTGIVVGHFRSGAMPV
                     HLLFSPTNGRALSPGEVYRLRVDEKKIVDILGRRLGDSTRVIEFRTVAEESLGTVTGK
                     VKASPRDGDVQVHLLFTGLTRLEVKRELTMRATAAGNPYSVALLSGKYRLSGYVDANG
                     NGRYDAGRVTPFLPAEPQTVLADTVNVRARFETEEVDLMFP"
     gene            1665..4427
                     /locus_tag="AC16FU_04425"
     CDS             1665..4427
                     /locus_tag="AC16FU_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993652.1"
                     /translation="MYRGKFTFGLVVFLLILQHRSLVTAGTPSFLLSPAQDVSSNTLA
                     TLSTVTDTLNSGRVGVADTHLIGFTLTTQQVPFDGKIIISFPPEFDLSAVKSVFYSDT
                     DFAHPDPSVDSIIITGQEITVLLDSTGQPAALGARINLLIPAIRNATVAGFYSVTVTI
                     ADRQGRILDGPNVSQPFTLSPGPAITLVISPDTDLAVSAGSVIPFQARAQDQFGNPIP
                     ASAFTWALLFTPDSIGQLTGSSLLATKTGTGRVEVTSGTLKDTSGLITVTPAEAARFM
                     LTGTPDTVNAGQGWPGQVTVSVFDRFDNRKTDYVGAVYFLGTDVTDSVPYGPTNRYQF
                     LPADNGQKSFLGTQFKFFSAGSHILTVGDDLLTASSAPIQVLAGPLAAFAVQAPVDVA
                     AGQPVPLSLLNANDGFGNALSGQANVNFVLGGSLSPFGKSPVLTPIAYTNGTGSATQI
                     LVATEKVVLRVTASGISRQTDTIRVRPGTLDTLLFNLSSPQDIGATFSGTALVRALDI
                     CGNTVTDFNAVADSVVIMPDGAGGMANNVLKQPTDFSTGVANLTAKGTTYLGQGGLVR
                     FTAVSQSGKRGRSDFVVMQGLSVDTLRVFPKLVRRGQEIGVEVFLFNSSTVTAQVTAV
                     ELLSDRGLIGGLAPAGGYPVSIGFNASARVADTLILPFDFPPGVAYLGVRVRLTYNTT
                     PLTLQLQDLDSLTVAAPANLNYVVASVTPSLLTRGTDVTLSLQLLNSGTFQVLLDTNT
                     RLVLRHQNGDSLATHLAQATPVFGGGIAPAELQFAPVGVPLTFPRGYYRITSLQVEGS
                     ENQVAFAQSVPTNDSVLVESVVQTSEFTATPNPFSPHGGPVQFEYVLAQPSAVRLKIF
                     SLLGDLAFEQEYPAGTNGGQLGRNVILWNGTNEDGRFVANGVYVGLLTVEGSGETRKV
                     KVAVAK"
     gene            4424..>5338
                     /locus_tag="AC16FU_04430"
     CDS             4424..>5338
                     /locus_tag="AC16FU_04430"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993653.1"
                     /translation="MKKPTPPLPSPQRGGKRKTFITSPWPSPARRGNVRIASSRPAKW
                     LFALALCWVSVGNAWGASDAGRRSLFSFGAGARAQGMGGAFVSVADDASAVFWNPAGL
                     LQLDQGKGLLQHATLFEGDFQFAGVAYPTLDLGTFGVGFMRIGTGDIPGRDRLGQPTG
                     TFSFAQSELVFAYGKKLFPYLGVGASVKAVAQELGDANDVGFGADVGLLLPRWRGLGL
                     GLTWQDAIGARLTLNQSREVMPWNLKGGLSYERFVAADRVGLLFSADLDQVQGGQARV
                     RVGGEVAIRRQLFLRAGLDKDEITFGAGV"
BASE COUNT         1002 a         1480 c         1679 g         1177 t
ORIGIN      
        1 tgcactggaa ggggaagatt ctggagatca agccgtctct cttctgggag ggagaacgga
       61 cgtacacgtt taccttcggg ggtcaggcgc aagacgggca cggcatccgg atgggggaga
      121 catttacttt ctccttttcc accaatacgg tgattgactc gggcaggatt gaagggttgg
      181 ttttcgccgg cttggtcccg gaagtgggag ccagcgtgtg tgcataccgg ctaaacagca
      241 ccgcggacat tgatccgatg cgccaaaagc ccgagtatgt gacggtgagc ggctcttccg
      301 ggaagtttcg cctttccgct ttggcggacg gccggtatcg cctgttcgcg gtcaaagacc
      361 agaaccgcga tcggctgtgg cagccgaaac gggagccctt tgccgtcggc accggagaga
      421 tcaccctcga ttcaaattcc cgacaccaga acggcttaca gttcttcctg gcggagcagg
      481 atacggcggc tttcggtctg gtgcggtgcc agaacttagg gcccttcgtg gtgtggctgg
      541 agttcaacca gccgctcgat tttggggcct ggaactggca ggagaatttt gaactgcggg
      601 agcgctccga ttccgcaggc cgccggtcag atgtgacaaa gatcactcgc gccgcaggga
      661 tgtatgaatt cgacgccgaa ccgaaaaatc tctatttagt gacggagcgc ctgtccatcg
      721 gttccaccta cgaccttatt ttgaagagtt tcactgaccg ctacgggaag ccgctggatt
      781 cagccagagc gcactgccgg ttcacggtgg cggctgaacc ggcaccgccc ctgcgtattg
      841 tccaaaccat tcccagaagc ggcgaaaagt attttgatcc gggacaactg ccgcttttgg
      901 tcgtgaacta tcctttgaaa agtcccgtgg acgatgcggc ggtgctcctc caagacacca
      961 cgggaatagt cgtggggcat tttcggagcg gggcgatgcc ggtgcacctt ttgttttccc
     1021 cgaccaacgg gcgggcgctc tcgccggggg aggtataccg tttacgcgta gacgaaaaga
     1081 aaattgtaga catcctgggc cggcggctgg gggattcgac gcgcgtgatc gagttccgca
     1141 ccgtggccga ggagtcgctg gggacggtca caggaaaggt aaaggcttcg ccgagagacg
     1201 gagacgttca ggtccacctc cttttcacag gtctcacgcg cctggaggtg aaacgggagt
     1261 taaccatgcg ggctaccgct gcgggaaatc cgtactcggt cgccctgcta tccgggaagt
     1321 atcggctgtc cggctacgtg gatgccaacg ggaacgggcg gtacgatgca gggcgcgtga
     1381 ccccattcct gccggcggag ccgcagacgg tactggccga tacggtgaac gtgcgggcgc
     1441 ggtttgaaac ggaggaagtg gacttaatgt ttccatgaaa gaagcagctg tggcgatttt
     1501 tggtgagcaa actcaaaaat ttctttcggt tttccgcgtg gctgattata ctctctttag
     1561 ggagatggcg gaattgagcg aggcgggaat ctcgctggtg ccggtgccac ggatggcgat
     1621 taggtcagtg gccccgccaa tggcgggacg aaccttgagg gaggatgtac cgaggtaagt
     1681 tcacttttgg tctggtagtt tttctcctca ttcttcagca ccggtcactg gtgacggcgg
     1741 gaacgccgtc atttttgctt tcccccgccc aagatgtatc ctcaaacact ctggcgacac
     1801 tttccaccgt taccgacacg ctcaactccg gccgcgtggg ggtggccgat acccacctga
     1861 tcggtttcac cctcaccacc cagcaggtgc cgtttgacgg caaaatcatc atttcgtttc
     1921 cgccggagtt tgatctctcc gcggtcaaaa gtgttttcta ctccgacacc gactttgctc
     1981 atccggatcc ttccgtcgat agcatcatta tcaccggtca agagatcacg gtgcttctgg
     2041 acagtaccgg gcagccggcg gctttggggg cgcggatcaa tctgcttatc ccggcgattc
     2101 ggaacgcgac cgtggccgga ttctattcgg tgacggttac cattgccgac cggcaaggga
     2161 gaattctgga cggccccaac gtctcgcagc cgtttaccct ctcgccgggc ccggccataa
     2221 ctctggtcat ttctccggac actgatctgg ccgtgagcgc ggggtcggtg ataccgttcc
     2281 aggcacgagc gcaggatcaa tttggcaacc cgatacccgc atccgctttc acctgggcac
     2341 tcttgtttac cccggattct atcggtcaat tgaccggaag cagtcttctg gcgacgaaaa
     2401 ccgggaccgg gcgggtggaa gtgacctccg gtactcttaa agacacgagc ggattgatta
     2461 cggtcactcc ggcggaggcg gcgcgtttca tgctcacggg gacgccggat acggtgaatg
     2521 cgggacaggg ctggccggga caggtgacgg tgtcggtttt cgaccgattt gataaccgca
     2581 aaaccgacta tgtgggagcg gtatactttc tgggtacgga cgtcaccgat tcggtgccct
     2641 acgggccgac gaaccggtac cagtttttgc cggcggacaa cggacagaaa agttttctcg
     2701 gcactcagtt caagtttttt tccgcggggt cacacattct cacggtgggg gacgacttat
     2761 taactgcttc cagtgcgccg attcaggttt tggccggacc gctggctgca tttgcggtac
     2821 aagcaccggt tgatgtagct gccggacagc ccgtgccgct ttcgcttttg aacgccaacg
     2881 atgggttcgg caatgctctc tcaggccagg caaacgtcaa cttcgttttg ggtggaagtc
     2941 tttcgccttt cggcaaaagc ccggtactca cgcccatcgc ctacaccaac ggcacgggga
     3001 gcgcgacgca aattctcgtg gcgacggaaa aagtcgtgct gcgggtaacg gcctccggaa
     3061 tcagccgcca gaccgacacg attcgggtgc gcccgggtac tctggataca ctgctcttta
     3121 atctgtcatc accgcaggat atcggtgcga ctttcagcgg cacggcgctg gtgcgcgcgc
     3181 tcgatatttg cggcaatacc gttaccgatt tcaacgctgt agccgatagc gtcgtgatca
     3241 tgcccgacgg agccggtgga atggcaaata acgtcttaaa acagccgacc gatttttcaa
     3301 ccggagtggc caatctgacg gccaaaggca ctacgtacct cggccagggc gggctggtgc
     3361 ggtttaccgc ggtcagccag tcggggaagc ggggccggtc ggacttcgtc gtgatgcagg
     3421 gtttgagtgt ggacaccttg cgggttttcc cgaaactggt gcggcgcgga caggagattg
     3481 gcgttgaagt ttttctcttc aactcttcca ccgtgacggc tcaggtcacg gcggtggagc
     3541 ttttaagcga ccgcgggctc atcgggggcc tcgctccggc gggcgggtat ccggtgagta
     3601 tcggtttcaa cgcttcggcc cgcgtcgccg atacgctcat cctccccttc gatttccctc
     3661 ccggagtggc gtacctgggc gtgcgggtgc gtttgaccta caacacgact ccgctcacgc
     3721 ttcagttgca ggacttggac agtctcaccg tggcggcacc ggcaaatttg aattacgtcg
     3781 tggcttcggt tacaccgtct cttttgaccc gcggcaccga cgtaaccctg tcgctgcaac
     3841 ttttgaattc aggcaccttc caggtgcttc tggacacaaa cacccggctg gtgctgcgcc
     3901 accagaatgg cgattcgtta gccacgcacc tggcgcaggc gacgccggtc ttcggtgggg
     3961 ggatcgcccc tgcggagttg caatttgcac cagtgggtgt gcccctaacc tttccccggg
     4021 gttactaccg cattacgtcc ctgcaggtgg agggatcgga aaatcaggtg gcgtttgccc
     4081 agagcgtgcc gaccaacgat tcggtcttag tcgagtcggt ggtgcaaacg agcgagttta
     4141 ccgctacgcc taatcccttt tcgccccacg gagggccggt gcagtttgag tatgtgctag
     4201 cgcaaccctc ggcggtccgg ttgaaaatat tctcgcttct gggggatctg gcgttcgagc
     4261 aggaataccc ggcggggaca aatggcgggc agttggggcg gaatgtcatt ctctggaacg
     4321 gcaccaatga agatgggcgg ttcgtggcca atggtgtgta tgtcggtttg ctcacggtag
     4381 aagggagcgg cgaaacgcgc aaagtgaaag tggcggtggc gaagtgaaaa agccgacccc
     4441 tcccttaccc tcccctcaaa ggggagggaa aagaaagacc tttataacct caccctggcc
     4501 ctctcctgca aggagaggaa acgttcgcat tgcttcctcc cgcccagcga agtggctttt
     4561 tgctctcgct ttatgctggg tatcagtcgg taatgcttgg ggagcttcgg atgcagggcg
     4621 gcggtcgctt ttctccttcg gcgccggggc gcgggcgcag gggatgggcg gggcgtttgt
     4681 ttccgtagcc gacgatgcct cggcggtgtt ctggaatccc gcgggactcc tgcaattgga
     4741 ccagggtaaa ggacttttgc agcatgccac gcttttcgaa ggagattttc aattcgccgg
     4801 agttgcctac cctactttgg atctgggaac tttcggggtg ggctttatgc ggatcgggac
     4861 gggcgacatc ccgggccggg accgcctggg tcagcccacc ggaacttttt ctttcgcgca
     4921 gagcgaactt gtcttcgctt acggtaagaa gctttttccc tacttaggcg tcggcgccag
     4981 tgtcaaagcg gtggcgcagg agttgggtga cgcaaatgac gtgggcttcg gcgccgatgt
     5041 cggtttactt ctcccgcgct ggcggggatt ggggctcggc ttaacctggc aggatgcaat
     5101 cggcgcccga ctgaccctga accagtcgcg ggaagttatg ccgtggaatc tgaagggcgg
     5161 actcagttac gagcgttttg tcgctgcgga tcgagtcggt ctgctctttt ccgccgacct
     5221 ggatcaggtg cagggcgggc aagcgcgggt gcgcgtcggg ggagaagtgg cgatccgccg
     5281 gcaattattc ctccgggcgg gcttggacaa agatgaaatc accttcgggg ccggcgtc
//
LOCUS       DBNGZA010000214         1701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2055079, whole genome shotgun sequence.
ACCESSION   DBNGZA010000214 DBNGZA010000000
VERSION     DBNGZA010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2055079"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1701
                     /gene="ftsH"
                     /locus_tag="AC16FU_04435"
     CDS             <1..>1701
                     /gene="ftsH"
                     /locus_tag="AC16FU_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR2993654.1"
                     /translation="LVVWMVKILNAGRGEVTRISYTEFMGQLDTNNIKTVTFVDKDLQ
                     GEFKEKFEKKEGRFLRKYGRFKLHIPFDDPQLLEKLRAKSVEIAAEPRGWDWGAALLS
                     MSPFLVLLVFWLLMLRQMQGGPKGLFSFGKSRARLLTEDKPKVTFSDVAGADEAKEEL
                     REIIEFLREPAKFQKLGGKIPKGALLLGPPGTGKTLLARAVAGEAAVPFFSMSGSDFV
                     EMFVGVGASRVRDLFDQGKRSAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLV
                     EMDGFESNEGVILLAATNRPDVLDPALLRPGRFDRQIVVDSPDVRGREGILKVHTRKI
                     PVGEDVELSVLARGTPGLSGADLANLVNEAALLAARRNHPKVTMVDFEDAKDKVMMGT
                     ERRSLVISDDEKRTTAYHEAGHALVAKLIPGSDPVHKVTIIPRGLALGLTSYLPFDEK
                     HTHSKQYLETRLVHMMGGRVAERLVFNQLTTGAGNDIEQATVLARKMVCDWGMSEKLG
                     PLTFGKKEEEIFLGREIAKHRDYSENTAQEIDKEVKKIVEEAERRATDLLSVNLEKLH
                     "
BASE COUNT          366 a          476 c          543 g          316 t
ORIGIN      
        1 tacttgtggt ctggatggtg aaaatcttaa acgccggccg cggggaagtg acccgaattt
       61 cgtacaccga gttcatgggg cagttggata cgaataacat taagaccgtc accttcgtgg
      121 ataaggacct ccagggggaa ttcaaggaga agttcgagaa gaaggaaggc cggttcctcc
      181 gcaagtacgg ccggttcaag ttgcacatcc cgtttgacga cccgcagctc ctggagaaat
      241 tgcgcgccaa atcggtggag atcgccgccg agccgcgcgg ctgggactgg ggagcggcct
      301 tgctctcgat gtcgcccttc ctcgtgcttt tggtcttctg gcttttgatg ctgcgccaga
      361 tgcagggcgg gcccaagggg cttttctctt tcggcaagag ccgggcccgg cttttgaccg
      421 aggataaacc gaaggtgacc ttcagcgacg tggccggggc ggacgaagcc aaagaagaac
      481 tgcgcgaaat catcgaattt ttgcgggagc cggcaaaatt tcaaaaatta ggcgggaaga
      541 ttccaaaagg agcgctcctc ttgggcccgc cgggcacggg caaaacgctt ttggcgcgcg
      601 ctgtggccgg cgaagcggcg gtgccgtttt tctccatgag cggctcggat ttcgtcgaga
      661 tgttcgtcgg cgttggcgct tcccgcgtgc gcgacctgtt cgaccagggc aagcggagcg
      721 cgccctgcat cattttcata gacgaaattg acgctgtagg ccggcaccgg ggcgctggtc
      781 tgggcggcgg gcacgacgaa cgggagcaga cgctcaacca actcttagtg gaaatggacg
      841 gttttgagtc gaacgaaggg gtcatcttgc tggctgccac caaccgtccc gacgttcttg
      901 atcccgccct tttgcggccc ggccgctttg accggcagat cgtggtggac agccccgacg
      961 tgcgaggccg cgaaggaatt ctgaaagtcc acacccgtaa gattccggtg ggtgaggatg
     1021 tagaattgtc ggttctggcc cgcggcactc ccgggctttc gggcgccgac ctggccaacc
     1081 tggtgaacga agcggcgctc ttggcggcgc ggcggaatca tcccaaagtc accatggtgg
     1141 atttcgaaga cgccaaagac aaggtgatga tgggaaccga aaggcggagt ctggtcatct
     1201 ccgacgacga gaagcgcacc accgcctacc acgaagcggg gcacgctctg gtggccaagt
     1261 tgatccccgg ctccgacccg gtccacaagg tgacgattat tccgcgcggc ctcgctctgg
     1321 gactcacctc ctacctgccg tttgacgaga agcacaccca ttccaaacag tacttagaga
     1381 cccgcttggt gcacatgatg ggcggccggg tggcggagag gttggttttc aatcagctca
     1441 ccaccggcgc cggcaacgac atcgagcagg ccacagtctt agcccgcaaa atggtctgcg
     1501 actgggggat gagcgagaag ttggggcctt tgaccttcgg caaaaaagaa gaggagattt
     1561 tcctgggccg ggagatcgcc aagcaccggg actacagcga aaacacggcg caggaaatcg
     1621 acaaggaagt gaaaaagatc gtggaagaag ccgagcgccg cgccaccgac ctcctctcgg
     1681 tcaatctgga aaaactgcac c
//
LOCUS       DBNGZA010000215         5260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1631552, whole genome shotgun sequence.
ACCESSION   DBNGZA010000215 DBNGZA010000000
VERSION     DBNGZA010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1631552"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2711
                     /gene="nuoG"
                     /locus_tag="AC16FU_04440"
     CDS             36..2711
                     /gene="nuoG"
                     /locus_tag="AC16FU_04440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR2993655.1"
                     /translation="MSDKTFTITVDGKKIEARPGETILEAALRSGIQIPTFCWHPKLD
                     PVGACRICLVKVEKSPKLVVSCATPAVAGQVVSTNSPEVIKGRQGVLEFLLINHPLDC
                     PTCDKGGECDLQDLTFRYGVDLSRYLEHKFRWAEQNTFDEISLGPMVLRNQNRCIHCY
                     KCTRVIQEVCGEHDLGAYQRGYRTEIHHWPAKAFEDQYAGNAVEVCPVGALTAKDFRY
                     KVRVWLTKQTKSVCQHCSDGCNTLLWVDPERNFLSRVTSRRNDCVDEGFLCSRGKFQY
                     QEANSPERLKTPLIRKDGRLVEVTWDEAYATLAKRLKDLKAKNGGESLAGIASATTPN
                     EDLYLFSRFLRTLVGSNNLDFRVNWGTNLPDPDLFYKFNRFSVAGVEQKKMIFVFGCD
                     FNREHPIITLRIRKASRKFGARVVLANPYRTKLGDSADVEAVYRFGSEVTFVNSLCQE
                     IIRQKLYPKEGYFCGEAIPEDFIQHLTGFPADQTAKVTGVDSEQVERLARALAGQANM
                     AILCGNGVLQHPQRNELVAALVNLAALLIGPSKVAQSLWLLPEEANFQGALDTGFLPD
                     QLPGNQPLADSQARAKCEQAWKKSISQSAGLSISQIIEATKSGKIKTWWVIGGEPTWQ
                     YPDHAAVEEAVKKVECLIVQANFRTPVVERADIVLPSAMLFERDGSFTSAEKRVQRFR
                     AGMKPVGQAKADWQIFSELAWWLEEPFDLFSAEEVFDEMAKLVPQYAGLNCKLLGDQG
                     LRWAEVNPEKEWTHKFTKVSADPIVAPPGDYPFLLLSGNSLYQSGFVYARTENLLRQE
                     PHPTVELSSETLASFACEEGDWVEIASPSGKVRVTAKENPYLSADVGIFHDNYPEARF
                     NFLTDESRDFCRVKLSKAPQPVTAQVPEPALAGVR"
     gene            2708..3703
                     /gene="nuoH"
                     /locus_tag="AC16FU_04445"
     CDS             2708..3703
                     /gene="nuoH"
                     /locus_tag="AC16FU_04445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR2993656.1"
                     /translation="MSNPVFIDALITVIKVFIVLNVILVSCAYLTRMERKVLARMQVR
                     LGPNKVGPGGIFQPFADLIKLIVKEDIVVTAGNRVLYILAPLLSWIPAFITFAVIPFG
                     TSVTIFGHKVDLVISDFNIGILYIFAATALGVYGIVLAGWSSNNKYSLLGALRSAAQM
                     ISYEVSLGLSVVGILLVAGTTSMLDIVAQQDSFFHWFIFKQPVGFLLYLTCAIAETNR
                     APFDLPEAESELVAGYQTEYSAMKFGMFYVAEYSNMITVAGIATTLFLGGWQGPFLPP
                     FLWFVLKVFVLLFLYIWIRATLPRFRYDQLMNFGWKVLLPLGLLNIVVTALLTLW"
     gene            3731..4273
                     /gene="nuoI"
                     /locus_tag="AC16FU_04450"
     CDS             3731..4273
                     /gene="nuoI"
                     /locus_tag="AC16FU_04450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR2993657.1"
                     /translation="MQNPVTSTWAILKGLYTTFIHLFRKPITVDYPVKKEPMKPRYRG
                     LHFLERYEDGSERCVCCGLCAAACPADCIYMEPAENEKGERYAKVYEINELRCIFCGY
                     CEEACPEEAIFLGREYEFSADDRNKFIYTKEDLLVPWPRIKPPPEFVRKERRVYSVKD
                     VEADLNKFKPSDRRLTEGTR"
     gene            4315..4830
                     /locus_tag="AC16FU_04455"
     CDS             4315..4830
                     /locus_tag="AC16FU_04455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR2993658.1"
                     /translation="MELTIFVITGIVALVSAVLVIAQRDPIRSVIFLIVTLCAQAVLY
                     LVLSAPFVAALQVIVYAGAIMVLFLFVVMLLNLGRDQFGKDPRRWQKRVGILFGLILV
                     LQFGLILRKGLFGRPVRAHPVLSTDYGSVAHVGKSLFTDYLYPFEITSVLLLVAIVGT
                     VILAKKKLEET"
     gene            4827..5129
                     /gene="nuoK"
                     /locus_tag="AC16FU_04460"
     CDS             4827..5129
                     /gene="nuoK"
                     /locus_tag="AC16FU_04460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR2993659.1"
                     /translation="MIPLSYYLILSGILFAIGVTGVLVCRNAIVIFMSIEIMLNAVNL
                     TLIAFAHYWNQADGQVFVFLVMTVAAAEAAVGLAIILNIFRQKESINIDDVNLMKW"
BASE COUNT         1090 a         1430 c         1528 g         1212 t
ORIGIN      
        1 agcgtccgaa gggtagaact tttgacgtga caagcatgag cgataaaacg ttcaccatca
       61 ccgtagacgg caagaagatc gaagccaggc cgggcgagac gattttggaa gcggcgctgc
      121 gatctggcat ccagattccc accttctgct ggcacccgaa actcgacccg gtgggggcct
      181 gccggatttg cctggtgaag gttgaaaagt cgccgaagtt agtggtttcc tgcgctacgc
      241 cggcggtggc gggacaggtt gtgtccacta attccccgga ggtaatcaag ggccgccagg
      301 gggtgttaga gtttttactc atcaatcatc cgctggactg tcccacctgc gacaagggcg
      361 gcgagtgcga tctgcaggat ttgacctttc gctacggcgt cgatctctcg cgctacctcg
      421 aacacaaatt ccgctgggcg gagcagaata cgttcgacga aatttccctg ggcccgatgg
      481 tgctccgcaa ccagaaccgc tgcatccact gctacaagtg cacccgggtg attcaggaag
      541 tctgcgggga gcacgacttg ggcgcctacc agcgcggcta ccggaccgaa atccaccact
      601 ggccggccaa ggcgttcgaa gatcagtacg cggggaacgc ggtggaggtc tgcccggtcg
      661 gtgctttgac cgccaaagat tttcgctaca aggtcagagt ctggctcacg aagcaaacga
      721 aatcggtctg ccagcactgt tcggacggct gcaacaccct gctctgggtc gatccggagc
      781 gaaacttcct ttctcgggtc acctcccggc gcaacgactg cgtggacgaa gggtttttgt
      841 gcagccgggg aaagtttcag taccaggaag ccaactcacc ggagcgcctg aaaacacctt
      901 tgatccgcaa agatggccgc ctggtcgaag taacctggga tgaggcgtat gcaacgctgg
      961 ccaaacgtct gaaggacttg aaagcgaaaa acggcggaga gagtcttgcc gggattgctt
     1021 cggctacgac gccgaatgaa gacctctacc tgttcagccg cttcctgcgc acgctggtcg
     1081 gctcgaacaa tcttgatttc cgggtcaact ggggtacaaa cctgcctgat cctgatttat
     1141 tctacaagtt taaccgcttt tccgtcgctg gtgtggaaca gaaaaaaatg attttcgttt
     1201 tcggctgtga tttcaaccgc gagcacccga tcatcacgct ccgcatccgc aaggcctccc
     1261 gcaagttcgg tgcgagggtc gttctggcga atccctaccg cacgaaattg ggcgacagtg
     1321 ccgacgtgga ggcagtctac cgcttcggtt ctgaagtaac gtttgtcaat tcgctttgcc
     1381 aggaaatcat tcggcagaaa ctctacccga aagaaggcta tttctgtggt gaggccattc
     1441 ccgaagattt cattcagcat ttgaccggtt ttccggcgga ccaaaccgca aaagttaccg
     1501 gcgttgattc ggaacaagtt gagcggttag cacgggctct ggccggccaa gccaacatgg
     1561 ccatcctctg tggtaacggc gtacttcagc atccccagcg gaacgaattg gtcgctgcgc
     1621 tggtcaatct tgcggcactg ctgatcgggc cgtccaaggt tgcccagtcg ctctggttat
     1681 tgccagaaga agccaacttc cagggagcgc tggataccgg ttttctgccc gaccaattgc
     1741 ccggcaacca gccgcttgcc gactcgcaag cgagagcgaa gtgtgaacaa gcctggaaaa
     1801 agtcgatctc ccagagcgct ggtttgagca tttctcagat tatcgaagcg acgaaatccg
     1861 ggaaaatcaa aacctggtgg gtgattggcg gcgagccgac ctggcagtac ccggaccacg
     1921 cggcggtgga agaggcggtc aaaaaagttg aatgcctcat cgtgcaggcg aatttcagaa
     1981 cgccggtggt ggagcgggcg gacattgttt tgccctcggc gatgcttttt gagcgcgatg
     2041 gttccttcac cagcgccgag aagcgggtgc agcggttccg cgcaggaatg aaaccggtgg
     2101 gccaggccaa agccgactgg cagatttttt ctgaattagc ctggtggctg gaggagccct
     2161 ttgacctatt ttccgccgaa gaagtttttg atgaaatggc caaacttgtt ccgcaatacg
     2221 ctggtttgaa ctgcaaactc ctcggcgatc agggactgcg ctgggcggag gtcaacccgg
     2281 aaaaagagtg gacgcacaag ttcacaaagg tgtctgccga tcccattgtc gcaccgccgg
     2341 gagattatcc tttcctcttg ctctccggca attcgctcta ccagtcgggc ttcgtttatg
     2401 cgcgaacgga aaatctccta aggcaggagc cgcatcccac cgttgaactt tcgtccgaga
     2461 ccttggcgtc tttcgcttgt gaagagggtg attgggtgga gatcgcttcg ccctccggaa
     2521 aggtgcgggt gacggccaaa gaaaatcctt acctttccgc cgacgtggga attttccacg
     2581 acaactatcc ggaagcccgg tttaatttct tgaccgatga aagccgggat ttctgccggg
     2641 tgaagttgag caaagcgccg cagccggtca ccgcgcaggt gccggaaccg gcgctggcgg
     2701 gagtgcgatg agcaatccgg tgttcatcga cgccctcatc acggtcatca aggtgttcat
     2761 cgtcttgaac gtgatcttag tttcttgcgc ttacctcact cggatggaac ggaaggtttt
     2821 ggcccggatg caggtacgcc tggggcccaa taaagtcggc cccggcggga ttttccagcc
     2881 cttcgccgat ctgattaaac tgatcgtcaa agaagacatc gtcgtgacgg cgggcaaccg
     2941 ggtgctctac atcttggcgc cgcttttgtc ctggattccg gccttcatca cctttgcggt
     3001 gattcccttc gggacatcag ttaccatttt cggccacaaa gttgacctgg tgatttccga
     3061 cttcaacatc ggcatcctct acatctttgc ggccacggca ctgggcgtct acgggatcgt
     3121 tctggccggg tggtcgtcca acaacaagta ttcgctcctg ggggcgttgc ggtcggcggc
     3181 gcagatgatt tcgtacgaag tgtccctggg gctttccgtc gtcggcattt tgctcgtggc
     3241 cggcaccacc tcaatgctcg atattgtcgc ccagcaggac agtttcttcc actggttcat
     3301 cttcaaacag ccggtcggct ttttgctcta tctgacctgt gccattgccg aaaccaatcg
     3361 ggcgccgttc gacctgccgg aggccgagtc ggaattagtg gccggctacc aaaccgaata
     3421 ctcggccatg aaattcggca tgttctatgt ggcagagtat tcgaatatga ttaccgtcgc
     3481 gggcattgcc accacgctct tcttgggcgg gtggcagggg ccgtttctgc cgcctttcct
     3541 ctggttcgtg ctcaaggtct ttgtgcttct gttcctctac atctggattc gggcgacctt
     3601 gccccgcttc cgctacgacc agctgatgaa cttcggctgg aaggtgctct tgcctctggg
     3661 acttttgaac atcgtcgtca ccgcgctttt aaccctgtgg tagtatcgag tcgaaattga
     3721 ggcgctgaaa atgcagaatc cggtaacgtc gacgtgggcc attctgaagg ggctgtacac
     3781 caccttcatc cacctcttcc gcaagccgat tacggtggac tatcccgtca aaaaagaacc
     3841 gatgaagccg cgctaccggg gactgcactt tttggagcgg tatgaggacg gctcggagcg
     3901 ctgcgtttgc tgcgggctgt gtgcggcggc ctgcccggca gactgcatct acatggagcc
     3961 ggcggaaaac gagaagggcg agcgctacgc caaggtatac gagatcaacg aactgcgctg
     4021 tattttctgc ggctactgcg aagaggcctg cccggaggag gcaatctttt tgggccggga
     4081 gtacgaattc tccgccgacg accgcaacaa attcatttac accaaagaag acctcttagt
     4141 gccctggccg cgcatcaaac cgccgccgga gtttgtacgc aaggaaaggc gggtctattc
     4201 ggtgaaagac gtggaagccg atttgaacaa gttcaagccg tcggacaggc gtttgactga
     4261 aggaacgcga taaggagcgg cgtccgggtc gtttgaccgg gtgggacaga agagatggaa
     4321 ctcacaattt ttgtcatcac gggcattgtg gctttggtct cggcggtttt agtcattgcc
     4381 cagcgcgatc cgatcagaag cgtcattttc ctcatcgtca ccctctgcgc tcaggcagtg
     4441 ctttacttag tgctctccgc gccctttgtg gcggcgctgc aggtgatcgt ctacgccggg
     4501 gcgatcatgg tattattcct ctttgtggtg atgcttttga atttaggccg cgaccagttc
     4561 ggcaaggacc cgcggcgctg gcagaaacgg gtggggattc tctttggctt gattttggtc
     4621 ctgcaattcg gcctgatttt gcggaagggg cttttcggca gacccgtacg ggcacatccg
     4681 gtgctctcca ctgattacgg ctcggtggcg cacgtgggga agtcgctttt caccgactac
     4741 ctctatccgt ttgagatcac gtccgtttta ctcttagtgg ccattgtcgg cacggtcatt
     4801 ctggccaaaa agaagttaga agaaacatga ttcctttaag ttactacctc atcttgagcg
     4861 gcatcctctt cgccatcggc gtgaccggcg ttttagtctg ccgcaatgcg atcgtcatct
     4921 tcatgtcgat tgagattatg ctcaacgccg tgaatttgac tttgatcgcc tttgcacact
     4981 actggaacca ggccgatggt caagtcttcg tctttttagt gatgaccgta gccgcggctg
     5041 aggcggcggt ggggttggcc atcattctga acatcttccg gcagaaagag tcgatcaaca
     5101 tcgacgacgt gaatttgatg aaatggtgaa gaaaagtcat tcgcagcaga tgaaacagat
     5161 ggagcggagg aagaaatccc cgaccggctc aggtcggtac gactctgcga ggacgagccg
     5221 gtctaccggt tcacgagtct gtcggtcgac gtggtggcac
//
LOCUS       DBNGZA010000216         7072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_42108, whole genome shotgun sequence.
ACCESSION   DBNGZA010000216 DBNGZA010000000
VERSION     DBNGZA010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_42108"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="tsf"
                     /locus_tag="AC16FU_04465"
     CDS             <1..979
                     /gene="tsf"
                     /locus_tag="AC16FU_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR2993660.1"
                     /translation="DRPGEGERSGPAKGRKSGPRGRQLRLEMAVTANLVKELREKTGT
                     GMMDCKKALEVTGGDLEKAVVYLREQGVAQAAKKVGRATREGTIYAALAPDYTAGTMV
                     EVNCETDFVARTAEFRALVEGIAREVSALDSDIASEKQLPVAEFLQRPLSSQDGRTVG
                     DLLTEGIARLGENLGLKQFARFSTRAGLVFAYIHPGDKLGVLVELATPAARGPAQEHI
                     RLLAKDVAMQTAAANPLVVSRAELPPDLTEKEKQIYRSQAVHEGKPEKVLNRIVSGKL
                     EKYFQEVCLLEQPFVKDQDRMVREVIAETEQKVGEKITVRRFARFRLGE"
     gene            1048..1770
                     /gene="pyrH"
                     /locus_tag="AC16FU_04470"
     CDS             1048..1770
                     /gene="pyrH"
                     /locus_tag="AC16FU_04470"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR2993661.1"
                     /translation="MPQTKYRRILLKISGEALMGAKEFGIDPDVIVSVAEQIKEVHEL
                     GVQIGVVVGGGNIFRGLAAGEAGIERVTGDQMGMLGTVINALALQDRLEKLGVYTRVM
                     SAIRMEAVAEPYIRRRAVRHLEKGRVIILAAGTGHPYFTTDTAAALRATEVEAEVILK
                     ATKVDGVYSADPKTDKNAKLFDTLSYLDVLSLGLKVLDATATSLCMDNQLPIIVFNLS
                     QRGNLKKIVLGDRIGTIVQNTR"
     gene            1803..2360
                     /gene="frr"
                     /locus_tag="AC16FU_04475"
     CDS             1803..2360
                     /gene="frr"
                     /locus_tag="AC16FU_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR2993662.1"
                     /translation="MIKQIYAETEEKMKKAVEVLSRELASTRTGKATTALLDGIRVDY
                     YGSHVPLNQVASLSVPEHRLLTIQPWERNLIPEITKAIQKSELGLTPSTDGNVIRLPI
                     PPLTEERRRDLVKHVKKLAEESKIALRNIRRDANEHLKKAKTDKKISEDDEKHAEKHV
                     QELIDKYIEAVDKLIKQKEAEIMEV"
     gene            2601..2933
                     /locus_tag="AC16FU_04480"
     CDS             2601..2933
                     /locus_tag="AC16FU_04480"
                     /inference="COORDINATES: protein motif:HMM:NF047646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR2993663.1"
                     /translation="MRQRGAFRLLAFVIMPDLLHLLLQPVNGKSLPNIMHSLKSYTAK
                     KLMRVLPGLRKVWQDGYYEHSIRTERELVEKIRYVESNPLKSGLCRDPEKFCFSSASK
                     REQMDTRT"
     gene            3029..3769
                     /locus_tag="AC16FU_04485"
     CDS             3029..3769
                     /locus_tag="AC16FU_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR2993664.1"
                     /translation="MSKERLRTEILARGNLPRHVAIIMDGNGRWAKKRGLPRTAGHKA
                     GVEAVRRTVRAAGELGIPYLTLFAFSVENWRRPREEVAAIMKLLYETTRRELRELMEN
                     NVRLIATGRLSELSPLRRAALRRAIEKTGANTGLTLNLALNYGGRTEILDAVRKACRE
                     ARAGRLNPEKLDEKLFANYLYTAGLPDPDLLIRTSGELRLSNFLLWQTSYTELYITDV
                     LWPDFSAEDFYRAIQEYQHRQRRFGGVA"
     gene            3819..4646
                     /locus_tag="AC16FU_04490"
     CDS             3819..4646
                     /locus_tag="AC16FU_04490"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR2993665.1"
                     /translation="MKNLAIRVAVATVAIPVILFICWRGGLWLFGFVLAISTLGLYEY
                     VRLIAPRYNLQRGWTFLLFSVGIPLVVHWYGEPALTPAVLVTFAILSLALVGTGQPVN
                     AVLGLGVAFLGIVYVPLLSSFLLLVRGMPDGMAGSSAFGGKWLILLFVTIWSCDTAAY
                     FVGKRFGKHKLAPVISPNKTIEGAVGGIIGGVAAAYLCQGTFFREALPKDALVVGMVV
                     GVIGQLGDLVESMLKRDAQVKDSSAIIPGHGGVLDRFDSLLFTLPCIYYYLKYFVYR"
     gene            4651..5568
                     /locus_tag="AC16FU_04495"
     CDS             4651..5568
                     /locus_tag="AC16FU_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR2993666.1"
                     /translation="MLRFWDYFFVLRPVLWLPVWTVSLLGARSALRHQAGTTLGTRSN
                     LLLILTGALLFGAVYLLNQIFDIESDRVNRKLRFLPEKLISVTAAVRETVLLNMAALI
                     VAFSVSIQTGWLAASIVILGILYSAPPFRLKNHPLGGLLANMLAHGSIVFWLGWSFIG
                     KLSGTAVLQSLPYFLSVGGIYLNTTVPDIPGDARAGKRTLAVIWGPKWTRLLAVLLVT
                     TAALAAYRVHEVPLLVAALASLPPFLLALLQNSTRLTILSTKLALVVLSAFACYYSPP
                     YLLILIVGFLATRLYYRRRFGLVYPSLGT"
     gene            5744..6199
                     /locus_tag="AC16FU_04500"
     CDS             5744..6199
                     /locus_tag="AC16FU_04500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR2993667.1"
                     /translation="MDLEPSVRSGPTVQTAQAIQTLPEAGNGIDPARLDSIISRYLGT
                     PYGQRQGRSRFDCSGFIREVFRNYSGRDLPASSRDMFKRLEPIASDQALCGDLVFFGN
                     NGAVSHAGIYRGDGTFAHASKSLGVTISSFAEPYYRQRYLGARRVPLSR"
     gene            6276..6536
                     /locus_tag="AC16FU_04505"
     CDS             6276..6536
                     /locus_tag="AC16FU_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2993668.1"
                     /translation="MLEAVKAMERFSPALKAKLVNLPDQPGVYLMKDAAGKILYVGKA
                     KVLKHRVRSYFQSASGGPWDPKVQALVSKIADFEILATDNEM"
     gene            6588..>7072
                     /locus_tag="AC16FU_04510"
     CDS             6588..>7072
                     /locus_tag="AC16FU_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR2993669.1"
                     /translation="MNLKDDKRYPYLKLTSEPFPRLLVVRRVKKDKARYFGPYTDVKN
                     MRKTLKLIGRIFQIRSCNLPLPPPPGKKFRVCLEYFIKRCPGPCEFKISQAAYQEMID
                     NAALFLEGKNVQLIKGLREKMEKYAAAEKFEQAALVRDQLLALESVVQKQKVVSQDEK
                     DR"
BASE COUNT         1500 a         2063 c         2021 g         1488 t
ORIGIN      
        1 ggaccgcccg ggagaaggag aaagaagcgg cccggcaaag ggaagaaaaa gcggcccgcg
       61 cggtcgccag ttgaggttgg agatggcggt tacggccaat ctggtcaaag aactccgcga
      121 gaaaaccggc accgggatga tggactgcaa gaaggcgctg gaggtgaccg gcggtgatct
      181 ggaaaaagcg gtggtctacc tccgggagca gggagtggct caggccgcca agaaagtggg
      241 ccgggcgacc cgggaaggca cgatctacgc cgccctcgcc ccggactaca ccgccggcac
      301 aatggtggaa gtgaactgcg agaccgactt cgtcgcccgc acggcggagt tccgcgcctt
      361 agtcgaaggc atcgcccgcg aggtaagcgc gctggattcg gacattgcct cagagaagca
      421 attgccggtg gctgaatttc tccagcggcc cctctccagc caggatggcc ggacggtggg
      481 tgaccttttg accgaaggca tcgcgcgcct cggagaaaat ctggggctca agcaatttgc
      541 gcgtttttcc accagggccg gcctggtctt cgcctacatc caccccggag acaagctcgg
      601 cgtcctggtg gaactggcga ctccggcggc tcggggtcca gcgcaagagc acattcgcct
      661 cctggccaaa gacgtcgcca tgcagactgc cgcggccaat cccctggtgg tcagccgggc
      721 cgaattaccg cccgacctga ctgaaaaaga aaaacagatt taccgctccc aggcggtgca
      781 tgagggcaaa ccggaaaagg tgctcaatcg catcgtgagc gggaaattag aaaagtactt
      841 tcaggaagtc tgccttttgg aacagccgtt cgttaaggac caggaccgga tggtgcggga
      901 ggtcattgcc gaaaccgagc aaaaagtggg ggagaaaatc accgtccggc gcttcgcccg
      961 cttccgcctg ggagagtgac gcgcgggagc cggacgtatt cgcacgggca gggaatgccg
     1021 gtaacaatcc gagagaagac tgaagcgatg ccccagacca agtaccgcag gattctgttg
     1081 aaaatctctg gcgaagcctt gatgggggcg aaagaattcg gcatcgatcc ggacgtgatc
     1141 gtctcggtag ccgaacaaat caaagaagtt catgagctgg gggtgcaaat cggcgtggtc
     1201 gtgggaggcg ggaacatctt ccgcggcctg gccgccggcg aggccggcat tgagcgcgtt
     1261 accggcgacc agatggggat gctgggtact gtcatcaacg ccttggccct gcaggaccgg
     1321 ctggagaaat taggagtcta cacccgggtg atgagcgcca tccggatgga agcggtggcg
     1381 gagccctaca tccgccggcg cgcggtgcgc cacctcgaaa aaggccgggt catcatcctg
     1441 gctgccggga ccggccaccc ctacttcacc accgacacgg cggccgccct gcgggccacc
     1501 gaagtcgagg ccgaagtgat cctgaaagcc accaaagtcg acggcgtcta ctccgccgat
     1561 cccaaaaccg acaagaacgc caaactgttt gacaccctgt cgtacttaga tgtcttaagc
     1621 ctgggactca aagtgctgga tgccaccgcc acctcgctct gcatggacaa ccagctcccc
     1681 attatcgtct tcaacctgag ccagcgcgga aatttgaaga aaatagtcct gggcgaccgc
     1741 atcggtacca tcgtgcagaa tacccgctga aggcatccgg aagggaggga accgagcgtc
     1801 cgatgatcaa gcagatttac gctgaaaccg aagagaagat gaaaaaagcg gtggaggtgc
     1861 tctcccgcga actggcttcg acccgcaccg gtaaggcgac gaccgcactc ctggacggca
     1921 tccgggtgga ctactacggg agccacgtgc cgctgaacca ggtggcctcc ttaagcgtgc
     1981 cggagcaccg gcttttgacc attcaaccct gggaaaggaa tttaattccc gaaatcacca
     2041 aagccattca gaaatcggag ctggggttaa cacccagcac cgacggcaat gtcatccggc
     2101 tgccgatccc cccgttgacc gaagagcgcc ggcgcgattt ggtcaagcac gtaaagaaac
     2161 tggcggagga gagcaaaatc gctctgcgca acatccgccg ggacgccaat gagcatctga
     2221 agaaagcaaa aacggacaag aaaatttccg aagacgacga gaagcacgcc gagaagcacg
     2281 tccaggagtt gatcgacaag tacatcgaag cggtggacaa attgatcaag cagaaggaag
     2341 ccgaaattat ggaagtctaa cccgagtttt tttgcgaagc gtgcctcacc cctctcgttc
     2401 gggaggggtt tttgttacaa tggtctcaaa tattctgggt aggccgggca tcctgcccag
     2461 ccaaagcggg gtcatttgag cagaccaaga cacatcgccg tcgcgaacgc agtgtactta
     2521 gttacgacgg taacccagaa ccggaatcct ctcttctctg aaccggcagc agcccaactc
     2581 gttcaaaacg cctttttttc ctgcgtcagc gcggcgcctt tcggctcttg gcctttgtca
     2641 ttatgcccga cctcctccat ctcctcttac aaccggtgaa tggaaaaagt ctgcccaata
     2701 ttatgcacag cttaaagagt tataccgcca agaaattgat gagggttcta ccgggtttgc
     2761 ggaaagtctg gcaggatgga tattacgaac acagcattcg caccgagcgt gaattggttg
     2821 aaaaaattcg ttatgtcgaa tccaaccccc ttaaatccgg gctatgtcgc gacccggaga
     2881 aattctgctt ttcatcggcg tcaaaacgag agcaaatgga tacgcggaca taaaaaccaa
     2941 aaatggcacc gggcaggatg cccggcctac ccgtcccaag aaatagcacc tgctttttca
     3001 aagacatttc tgttatactg acttttcgat gtcaaaagaa cgtctgagaa ccgaaatcct
     3061 ggcccgaggg aatctgcccc gccacgttgc cataattatg gacggcaacg gccgctgggc
     3121 gaagaaacgc gggctgcccc gtaccgccgg acacaaagcc ggcgtggaag cggtgcgccg
     3181 caccgtccgg gctgccggtg aactgggtat cccctacctc accctcttcg ccttttcggt
     3241 ggaaaactgg cgccggccgc gggaagaagt ggcggcgatt atgaaactcc tgtacgagac
     3301 tacccggcgc gaactgcggg agttgatgga aaacaacgta cggttgattg ccacgggtcg
     3361 cctgagtgaa ctctccccct tgcgccgtgc cgccctgcgc cgagccattg agaaaaccgg
     3421 agcaaatacc ggcttgacgc tcaacctggc gctcaattac gggggccgga cggaaatcct
     3481 ggacgcggtg cggaaagcct gccgggaggc ccgggccggt cgtctcaatc cggaaaaatt
     3541 ggacgaaaaa ctttttgcga attacctcta caccgccgga ctgcccgatc ccgatttgct
     3601 catccgcacg tccggcgagc tgcgcctgtc gaacttcctt ctctggcaaa cttcttatac
     3661 cgaactgtac atcaccgacg ttctctggcc tgattttagc gcggaagatt tctaccgcgc
     3721 aattcaggag taccagcacc gacagaggcg ctttggggga gtggcgtaat ggaaccgctc
     3781 tcttcccgca acgattccaa cccatccgct tgccgcttat gaaaaatctc gccatccgtg
     3841 tggccgtcgc caccgttgcc atcccggtga ttcttttcat ttgctggcgc ggcggacttt
     3901 ggctcttcgg cttcgtactc gccatctcga ctttaggctt gtacgagtac gtccgcctga
     3961 ttgccccgcg ctacaacctc cagcgcggct ggactttttt gctcttttcg gtcggtatcc
     4021 ccctggtggt gcactggtat ggcgaaccgg cgctgactcc ggcggtgctg gtgacgtttg
     4081 ccattttgag tctggcgctg gtcggcaccg gccagccggt gaacgcggtc ttggggctgg
     4141 gcgtcgcttt tctaggaatc gtttacgtgc cccttctgtc gagtttcctt cttttagttc
     4201 gcggtatgcc ggatgggatg gcggggagtt ccgccttcgg cggcaagtgg ctcatcctcc
     4261 tttttgtgac gatctggagt tgcgataccg ccgcctactt cgtaggcaaa aggttcggga
     4321 aacataaact ggctccggta atcagcccga acaaaaccat cgaaggcgcg gtcggcggta
     4381 tcatcggcgg cgttgccgcc gcctacctct gccagggtac gttcttccgc gaagccctgc
     4441 cgaaagacgc gctcgtggtg ggaatggtgg tcggcgtcat cggccagtta ggtgatctgg
     4501 ttgagtcgat gctcaagcgc gacgcacagg tgaaagattc ttcggccatt atccccggtc
     4561 acggcggcgt cctcgaccgt ttcgacagtc tgctcttcac cttgccctgc atttactact
     4621 acctgaagta cttcgtctac cgttagggtg atgctacgtt tttgggatta ctttttcgtc
     4681 ctgcggcccg ttctctggct gccggtctgg acagtttcac ttctgggcgc ccggagcgcg
     4741 ctccgccatc aggccgggac aaccttggga acaaggtcga atctgctttt gatcctgacc
     4801 ggtgcgctcc tcttcggtgc cgtctacctg ctgaatcaga ttttcgacat cgaatccgac
     4861 cgggtcaacc gcaaactccg ttttctgccg gagaaattaa tttcggttac tgccgctgta
     4921 cgtgaaacgg tactgctcaa catggcggct ttgatcgtcg ccttttctgt ttccattcag
     4981 acgggttggc tggccgcaag cattgtcatt ttagggatcc tctactcggc gcctcccttc
     5041 cggcttaaga accatcccct gggcggtctt ctggcgaata tgcttgccca tgggagcatc
     5101 gtcttctggt tgggttggag ttttatcggg aagctgagcg ggacggcggt gctccaatcg
     5161 ctcccctact ttttgtccgt gggcgggatt tatttgaata ctaccgttcc cgatatcccg
     5221 ggtgacgccc gcgccgggaa gcgcactttg gcggttatct gggggccgaa atggactcgg
     5281 ctgctcgccg tcttactcgt tacgactgcg gccctggcgg cgtaccgcgt acacgaagtg
     5341 ccgcttttgg tcgccgctct ggcttccctg ccgcctttcc ttctggcgct cttgcaaaac
     5401 agcacgcgct tgacaatcct ctccaccaaa ttggctttag tagtcctttc cgccttcgcc
     5461 tgttattact cccccccgta cctcttgatt ctgatcgtag gctttttggc aacccggctc
     5521 tactaccggc gccggttcgg tctggtgtac ccctctttgg ggacctgatt ccaatcggac
     5581 tcccgcagta atttcatctc aattttcagg ttggcttttc cgataaacga agaaggtcat
     5641 ttcaggagca aaaatgcgcc ctgtccttct cggcattctg ctgcttgttt tagccggtgg
     5701 ttgtgtaccg gctccgcggt atacgtctcg gccctggtca gacgtggacc tggagccttc
     5761 ggtccgatct gggccaacgg tccaaacggc acaggctata cagacgctcc cggaggccgg
     5821 aaacggcatc gacccggccc ggctggacag catcatatcg cgttacctgg gcacccccta
     5881 tggccagcgt caggggcgga gccggttcga ctgctccggt ttcatccggg aagtattccg
     5941 caattattcc ggccgggacc tccccgcctc cagtcgtgat atgttcaagc gactggaacc
     6001 gattgcctcc gatcaggcgc tctgcggcga tctggtcttc tttgggaata acggtgcggt
     6061 cagccacgcc ggaatttacc ggggagatgg caccttcgcc cacgcgtcga agtcactggg
     6121 ggtgacgatt tcttcgtttg ccgagcccta ctaccggcag cggtacttag gcgcccgccg
     6181 ggtgccgctc tcgcggtgat ctcgtgccac cacctttttc ccggagccgc tcctcgctct
     6241 cgcttttttc tgctgcagag cgtaagttta taggcatgct cgaagcggta aaagcgatgg
     6301 agcgtttcag tccggcgctc aaagccaaac tggtgaatct tcccgaccag cccggggttt
     6361 acctgatgaa agatgcggcg ggaaaaatcc tctacgtcgg gaaggcgaag gtcctcaagc
     6421 accgggtgcg ttcctacttc cagtccgcct caggcggacc ctgggatccg aaagtgcagg
     6481 cgctggtctc caaaattgcc gactttgaaa tcttagccac cgacaacgaa atgtaagcgc
     6541 ttatacttga ggcgaacctt atcaaagagc acaaaccgcg ctacaacgtg aacttgaagg
     6601 atgacaagcg ctatccctat ttaaaactga cctctgagcc gttcccccgc ctcttagtcg
     6661 tccgccgggt gaagaaggat aaagcgcgct acttcggccc ctacaccgac gtcaaaaata
     6721 tgcgcaagac gttgaaattg atcggccgga ttttccagat tcggtcgtgc aacctcccct
     6781 tacccccgcc gcccggcaag aaattccggg tttgcctgga atatttcatc aagcgttgcc
     6841 ccggcccctg cgagttcaaa atttctcagg cagcgtatca ggaaatgatt gacaatgctg
     6901 cgctgttcct cgagggaaaa aacgtccagt tgattaaggg tctgcgcgag aaaatggaaa
     6961 agtatgccgc ggcggaaaag tttgaacagg ccgcgctggt acgcgatcaa cttctggcac
     7021 tggagagcgt cgtccagaag cagaaggttg taagtcagga cgaaaaagac cg
//
LOCUS       DBNGZA010000217         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_677605, whole genome shotgun sequence.
ACCESSION   DBNGZA010000217 DBNGZA010000000
VERSION     DBNGZA010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_677605"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="miaA"
                     /locus_tag="AC16FU_04515"
     CDS             <1..659
                     /gene="miaA"
                     /locus_tag="AC16FU_04515"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR2993670.1"
                     /translation="EIAGRGRWPLVVGGTGLYLRALTQGLFQGPGADEKVRERLHKEA
                     LEKGTGFLHERLAKVDPKTAARLHQNDRTRIIRALEVYEVTGRPISEWQEKGQYPETE
                     FSFVQVGLQMERSRLYERIDRRVDQMLANGLRREVEKILASGVSPEAPGLKTVGYQEV
                     IVHLAGRCSWDKTVALIKQHSRNFAKRQLTWFRSQTDIIWLDAEAPDAARRVLACVPK
                     "
     gene            831..>2212
                     /locus_tag="AC16FU_04520"
     CDS             831..>2212
                     /locus_tag="AC16FU_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR2993671.1"
                     /translation="MKRIVAGFIFALLFSAGACSPVLKSKPPVSPPPSRTAPVASADS
                     AGSGSARRTDAGTPSPKVTGKETGLPAGSEENGPPESGEDQAGIDTTAIEPDLEEQLK
                     DIQCLYADGVSLLRERRWELAQAKFEEALDAIADLEIDDQTEPELYERANTLLKEIGG
                     DLKTALVAQGQLGEESSTASFLERFESIKNFKALREQAHLEPLARPDTVQFDVPIVWN
                     QRVQDALTYLQTVAREPFTRYLERMGKYLDLMRGIFRKKNVPLDLCYLPLIESGYSPH
                     AYSYAHAVGPWQFIASTGRLYGLKSTFWIDPRRDFVKSTEAAADFLGDLYQQYNSWYL
                     ALAAYNGGPGRIDRSMRRQKTDDFWKLDLRRQTENYVPLFLAATIIAKQPKKYGFDVV
                     AEAPLRYDIVTVDRSLELEAVAKAMSVDEEELRGLNPELVRGVTPPNLRGYDLRLPAG
                     STQLFLARYE"
BASE COUNT          498 a          624 c          694 g          396 t
ORIGIN      
        1 gggaaatcgc aggacgcggc cggtggccac tggtagtggg tggaaccggc ctttacctta
       61 gggcgctcac ccagggattg ttccagggcc cgggagccga tgaaaaagtg agggaaaggt
      121 tgcacaagga agcactggag aaaggaaccg gtttcttgca cgagcgctta gcaaaagttg
      181 accccaagac tgccgcccgg ctccaccaga atgaccggac gcggattatc cgggcgctgg
      241 aagtgtacga ggtgaccgga cggccgattt cggagtggca ggaaaaggga caatatccgg
      301 aaactgaatt ctcctttgtg caggtcgggc tgcaaatgga acggagcagg ttgtacgaac
      361 gtattgatcg ccgggtcgat cagatgcttg ccaacggatt aagaagggaa gtggaaaaga
      421 tattggcatc gggcgtatcg ccggaggcgc cgggattgaa aacggtcggc taccaggaag
      481 tgatcgttca tctggcaggt cgatgttcgt gggataagac ggtcgcattg atcaaacagc
      541 acagtcggaa ctttgccaaa cggcagttga cctggttccg cagccagacc gacattatct
      601 ggctggatgc tgaagccccg gatgcggccc ggcgtgtgct ggcctgtgtg ccgaaatgaa
      661 agaggaggag aattcctttg cgcacatacg gttatgcgca aaaaagactt gacagaaact
      721 cgacgacctt gtatgaagag aggcgctgtg cccgggacgg gcgaacgggt gcagttggag
      781 ttttgtgctc ttcggaagga gggcgcagga ccatcgcaaa ggatgagacg atgaaaagaa
      841 tcgtggccgg ctttatcttt gcactgctgt tttcggcggg tgcctgctcc ccggtcttga
      901 agtcgaaacc cccggtttcc ccgccgccat cccgcacggc acccgtggcc tctgcggaca
      961 gcgccggcag cggctcggcc aggcgcaccg atgccggaac tccctcgccc aaagtgaccg
     1021 ggaaggagac ggggcttccg gccggctcag aagagaacgg cccgcctgaa tccggtgagg
     1081 accaggccgg cattgatacc accgctatag agcccgattt agaggaacag ctgaaagaca
     1141 tccagtgcct gtacgcagac ggcgtctcgc ttctgcgcga gcggcgctgg gaactggccc
     1201 aggcgaagtt tgaagaagcc ttagacgcca ttgccgatct ggaaattgac gaccagaccg
     1261 aaccggaact ctacgagcgc gccaacaccc tgttgaagga aatcggcggc gacctcaaaa
     1321 cggccctggt ggctcaggga caactgggag aagagagttc caccgcctcg tttctggaac
     1381 ggttcgagag catcaagaac ttcaaagccc tgcgcgaaca agcccacctg gaacctctgg
     1441 ccagacccga caccgtccag ttcgacgtgc cgatcgtctg gaaccaaagg gtgcaagacg
     1501 cgctcaccta tttgcagact gtggcccggg agccattcac ccgctacctg gaacggatgg
     1561 gaaagtatct ggatttgatg cgcggtattt tcaggaagaa aaacgtgcct ttagacctct
     1621 gctacctgcc cctaattgag agcggctact ctccccatgc gtactcgtat gcgcatgcgg
     1681 tgggcccctg gcagtttatc gcctccaccg gacggctcta cgggctcaag tcgaccttct
     1741 ggatcgatcc ccggcgcgat ttcgtgaagt ctaccgaagc cgccgccgac ttcctcggtg
     1801 acctctacca gcagtacaat tcctggtatc tggcgctggc ggcttacaac ggcgggccgg
     1861 gccggattga ccggagcatg cggcgccaga agaccgacga tttctggaaa cttgatttac
     1921 gccggcagac cgaaaactac gtgcccctgt tcctggccgc caccatcatc gccaagcagc
     1981 cgaaaaagta cgggtttgac gtcgtagcgg aagcgccact gcgctacgac atcgtcaccg
     2041 tggaccgcag cctggaactg gaagcggtgg ccaaagcgat gagcgtggat gaagaggaac
     2101 tgcggggact caacccggaa ctggtccggg gcgtcacgcc gcccaatctg cgcgggtacg
     2161 acctgcgcct gccggccggg tcgactcaac tcttcctggc gcggtacgag ga
//
LOCUS       DBNGZA010000218         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2268497, whole genome shotgun sequence.
ACCESSION   DBNGZA010000218 DBNGZA010000000
VERSION     DBNGZA010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2268497"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..3020)
                     /locus_tag="AC16FU_04525"
     CDS             complement(240..3020)
                     /locus_tag="AC16FU_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2993672.1"
                     /translation="MHILKYVFRFGVLLLLYSPSSSFAANADCLSCHQEKDLTKTTAE
                     KTVKSLYVDQKKFENSIHGQLACVDCHADLAQTEFPHTEKVQPVDCGSCHGEVAEHYR
                     GSLHGQAVAAGAKLAPRCWDCHGAHDILPVQSPESRVRRFNVPFVCGRCHKEGTAVTR
                     TYNIPQDSILTHYSVSVHGEGLFKKGLTVTAICTDCHTAHNVLPHTDPHSTIYRENVP
                     KTCEKCHGLIEQVHRKVIRGELWEKEPNKVPVCIDCHAPHRVRKVFYELGMSDRDCLR
                     CHAQPRLFTIRNGDTISLSIDTVEMRNSIHRTTACAQCHTGASPVEKRPCATITTRVD
                     CSICHAEAAAVYATSTHGTLANRGDPNAPLCRDCHGVHGTKERRDPTSPTYPTHVPNL
                     CGQCHREGEKAALRLTGEKTKIVKNYIESIHGKGLLQSGLVVTAMCTDCHTAHHELPS
                     RDSASSVNRANIAKTCARCHNGIYEQFALSIHFLGKAKTDKPLPLCHDCHTSHTITRT
                     DQEGFKLKMISQCGRCHQDVAEGYFETYHGKVSKLGYTAAAKCYDCHGAHDVLPPWDP
                     KSRLSRQNIVKTCGQCHPGSHRRFAGYLTHATHHDPVKYPVLFYTFWSMTLLLIGTLG
                     FFGLHTILWLPRSFQAMRERKKLDLQSPGKEYLRFDTLSRRLHVLVIVSFIGLALTGM
                     TLKFSYLGWAQWLSRLLGGFESAGYIHRVCAVITFFYFGTHIFDLVRKKRRERKTWKE
                     VLFGPASMLPNKIDLLEFWGTFKWFVGLGPRPAYGRWTYWEKFDYFAVFWGVTIIGAT
                     GLFLWFPETFTHIFPGRFINMATIIHSDEALLAVGFIFTVHFFNTHFRPEKFPMDTVI
                     FTGRVPLEELKLDRPREYEELVTSGELEKRLVEPLPPVVVRGMKMFGWIALSLGLLMV
                     LLIVWAEIFGYR"
BASE COUNT          645 a          885 c          975 g          678 t
ORIGIN      
        1 accgcttgcc cggcgggagt atttttggtc agactgcggt cgccgtgaca ggtaaagcaa
       61 tcggcattct ctatccccca cgccgtcttg gagcagatca ggctgaagcc gatgagcact
      121 acggagtagt ataaattccg catttcagga tctcgaatcg ctgcctttcg ctcggcccac
      181 cggtaacttg tcgtctcgac accttttggt caatggctgg aactgcccgt cccgctatct
      241 taacggtacc cgaatatctc cgcccagaca atcaagagta ccataagcaa ccccaatgag
      301 agcgcgatcc agccgaacat cttcattccc cgcaccacca ccggcggcag gggctcgact
      361 aaccgcttct ccaattcgcc ggaggtcact aattcctcgt attcgcgggg acgatccaac
      421 tttaactcct ccaagggcac ccgtccggtg aagatcaccg tgtccatggg aaacttctcc
      481 ggccggaaat gggtgttgaa gaaatgcacg gtaaagatga acccgaccgc caaaagggcc
      541 tcgtcggagt gtatgatggt ggccatgttg atgaaccggc ccgggaagat gtgggtgaag
      601 gtctccggga accagaggaa taaaccggtg gcgccgatga tggtcacccc ccagaagacg
      661 gcgaaatagt cgaacttctc ccagtaggtc cagcgcccgt aggccggtcg gggtcccaga
      721 ccgacaaacc acttgaaggt tccccagaat tccaaaaggt cgatcttgtt cggcagcatc
      781 gaagccggac cgaagaggac ctccttccag gttttcctct cccttctctt cttccgaacc
      841 aggtcgaaga tgtgcgttcc aaaatagaaa aaggtgataa ccgcgcagac ccggtgaatg
      901 tatccggccg actcaaaccc gcccagcagg cgggataacc actgcgccca tcccaagtag
      961 gaaaacttga gcgtcatgcc ggtcaaagcc agcccgatga aactgacgat cacgagtacg
     1021 tgcaggcggc gggagagggt gtcgaagcgc aggtactctt tacccgggga ttggagatcc
     1081 agttttttcc gctcgcgcat ggcctgaaac gagcgcggca gccagagaat ggtgtgcaga
     1141 ccgaagaacc ccagagttcc gatcaaaagc aacgtcatgg accagaaagt gtagaagagc
     1201 accgggtatt tgaccgggtc gtggtgggtg gcatgggtga ggtacccggc aaagcggcgg
     1261 tgtgatcccg gatggcactg gccgcaggtc ttgacgatgt tctgccgcga gagccgggac
     1321 ttcgggtccc aggggggcag aacgtcgtgc gcaccatggc agtcgtagca tttggcggcg
     1381 gcagtgtagc ccagtttgga taccttgccg tggtaggtct caaaataccc ttccgccaca
     1441 tcctggtggc agcgcccgca ctgggaaatc attttcagct taaacccttc ctgatcggtg
     1501 cgggtgatgg tgtgcgaggt gtggcagtcg tggcagaggg gcaggggttt atcagttttg
     1561 gccttaccga gaaaatggat gctcagcgcg aattgctcgt agatgccgtt gtggcaccgg
     1621 gcgcaggttt tggcgatgtt ggcgcggttc acactggagg ccgaatcacg ggacggcagc
     1681 tcgtggtgcg ccgtgtggca gtcggtacac atggcagtaa ccaccaggcc gctctgcaac
     1741 agccctttac cgtggatgct ctcgatgtag ttttttacga tcttggtttt ctccccggtc
     1801 agccgtaggg ctgctttctc cccctcccgg tggcattggc cgcacaggtt gggcacgtgg
     1861 gtcgggtagg tcggtgaagt gggatcgcgg cgttctttgg tgccgtggac gccgtggcag
     1921 tcccggcaca aaggcgcatt gggatcaccc cgattggcga gcgtaccgtg ggtgctcgtg
     1981 gcgtagaccg ccgccgcctc agcatggcaa atcgaacaat cgacccgggt ggtgatggtg
     2041 gcgcagggcc gcttttccac cggactggca ccggtgtggc actgggcgca ggccgtcgta
     2101 cggtgaatcg agttgcgcat ctcgacggtg tcgatagaaa gcgaaatcgt gtcgccattg
     2161 cggatggtaa agagcctggg ctgggcatga cagcggaggc agtcccggtc cgacatccct
     2221 aattcgtaga aaactttccg cacccggtgg ggtgcgtggc agtcgatgca gaccggaact
     2281 ttattgggct ctttctccca cagttcgccc cggatcacct tccggtgcac ctgctcgatc
     2341 agcccgtggc acttttcaca ggttttggga acgttttccc ggtaaatcgt cgaatgcgga
     2401 tcggtgtgcg ggaggacgtt gtgggcggtg tgacaatcgg tacagatcgc cgtcaccgtc
     2461 agtcctttct tgaacaatcc ctccccgtga acgctgacgg agtagtgggt caggatgctg
     2521 tcctggggga tgttgtaggt tcgggtcacc gcggtgccct ctttatggca ccgcccgcag
     2581 acgaaaggaa cgttgaaccg gcgtacccgg gattccggtg actgcaccgg gaggatgtcg
     2641 tgcgcgccgt ggcaatccca gcagcgcggg gctaacttcg ccccggccgc taccgcctgg
     2701 ccgtgcagac tgccgcggta gtgttcggcg acttcgccgt ggcaactgcc gcagtcgacc
     2761 ggctgaacct tttcggtgtg cgggaattcc gtctgggcta aatcggcatg gcagtcgacg
     2821 cacgccaatt gcccgtgaat cgagttttca aatttcttct ggtccacgta gagggacttg
     2881 accgtctttt cggcggtcgt tttcgttaaa tctttttcct gatggcaact gaggcaatcg
     2941 gcgttggcgg caaaagaact ggagggcgag tataaaagta aaagaacccc gaatcgaaaa
     3001 acatatttta gaatgtgcac cgactactcc gacatcgtaa cgctaccgta tcggtaaaga
     3061 ttcaatcacc ggcttcggat tttcctcttc cgtcgccaaa agactgtggc actggtcgca
     3121 gtcctggcgg atggtttggc cgtctttgct ctggtggttg tcgtcgtggc agcggaagca
     3181 ccc
//
LOCUS       DBNGZA010000219         4427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1101332, whole genome shotgun sequence.
ACCESSION   DBNGZA010000219 DBNGZA010000000
VERSION     DBNGZA010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1101332"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="AC16FU_04530"
     CDS             complement(<1..1426)
                     /locus_tag="AC16FU_04530"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR2993673.1"
                     /translation="MPPPSGGLGAAPSASLDQIIDPNDYVVGPGDVLVLGVWGSFEQT
                     QSLVVTPEGTLLIPTLGEITVGGKTLAHVKEELKAFARGRYSNTTVTMTLVGMRRFKV
                     PVGGWVAKPGTYEVSAAMRVAEAIALAGGIRGDGSLRRIELTRSHGERFLCDLFRVQQ
                     ISDSRFNPRLREGDRIVVPARSYRGDCTVSGAVGTAITVEYREDDSLLCLLALAGGLT
                     EQADSSRVEITRLSNPHGPETFTIDLRAEQPDRAAVPGDVILVRTNPQILRQYAVWVS
                     GEVVSPGSYVIDPGQTKLSEVIQRAGGFTPRAALAQAAILRPAGLSGSRSSSDTLPTA
                     FLSQLSPEEIDYYKTRVLLRPGQVSCDFVRIFERGDLAADVIVQEGDYITVPSRSPSV
                     YVTGRIAQPGFVAHEPDRPLRYYIERAGGYSWNADPGKARVIKGVSGAWQKASPDTRV
                     DAGDTIWIPEKKKQKFLTAMRDNLL"
     gene            complement(1813..3708)
                     /locus_tag="AC16FU_04535"
     CDS             complement(1813..3708)
                     /locus_tag="AC16FU_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993674.1"
                     /translation="MRSYRKANSILAFLLLCLPIMVVPGHAEKAVRFSSQLVENYRGN
                     PPQAGVIDRREFVCPYNVRVNGLAGNFIFDLTVTPISKKMIGLELELFTFGGQPEHFL
                     RIDTLNWGETLTLASLPLKGRSTLQWTLVPSPAETTFECDTSPLDSTFWNYEGSPRFI
                     FYYRLWSLGDFQWNRWRDNLEDELDALQRTFDLTTPERVRFYYFPCQTKALVWDRRFP
                     FAVDPLRYRVCAVHQPGKSGVDPFPVILEKICHLWGYAPALLAEGLAGYLDYSHYYAQ
                     QDLPAGRLLKLATLSKSQEYYAQPAKEATIQAASFVRYLATTYGGARLEQLYRQATDL
                     SLDSTCTAVYGKPLEGLEAEWRAYLDTFPALKPDLNHFAERALRFGHYRDALELFQKL
                     VALDSTDEYEVGQLVLTHYLLGQYPQGLKLALTNLHFHPTAENQVVAANFFLLADQLD
                     SAVVHYRAAMQGDSLLADPYVRLGSFYVERGDESLGVPLLEEALRKRIADPDRAELSI
                     ALGQVAKRRGDLNEMAVALNNSWALLRNMRTEDPSNSFPYYLLGEMFLVKGFLDSAIA
                     NLQTAQFVEERPYYLGKIALALGKAYDLKKQRKEAVAAYQFGLNVPSIPFDRRRLERY
                     LKKPFRE"
     gene            complement(3786..>4427)
                     /locus_tag="AC16FU_04540"
     CDS             complement(3786..>4427)
                     /locus_tag="AC16FU_04540"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR2993675.1"
                     /translation="YLINIASSDKALNQKSREALAIEVERCEALQIPFLVLHPGSHLG
                     AGEEIGIKNIAGALDWVHVQTPRAKAKIALETTAGQGAHLGFKFEQLRDMIDLTKEKE
                     RLAVCLDTCHVFAAGYDIRTEKGYEQTIADFDRTVGLDRLGAIHFNDSKKGLGSRVDR
                     HEHIGKGFLGKEAFGFFLNDKRLAHIPKILETPKGPDYKEDKINLAVLRKLIK"
BASE COUNT          950 a         1354 c         1215 g          908 t
ORIGIN      
        1 ggagcagatt gtctcgcatg gcggtcagga atttctgctt ttttttctcg ggaatccaga
       61 tggtatctcc cgcatcgacc cgggtgtccg ggctggcttt ttgccaggca cccgagaccc
      121 ccttgatgac tcgcgccttt cccgggtcgg cattccagct atagcctccg gcgcgctcaa
      181 tgtagtagcg cagcgggcgg tcgggttcgt gggccacaaa accgggttgc gcgatccgac
      241 ccgtgacgta caccgaggga ctccggctcg gaaccgtgat gtagtccccc tcctggacaa
      301 taacgtcagc ggcgaggtcg cctctttcaa atatccgcac aaaatcacag gagacctgtc
      361 ccggacgcaa aagcacgcgg gttttgtagt aatcaatctc ctcaggtgag agctggctca
      421 ggaaagcggt cggcagggtg tcactgctgc tgcgggagcc ggacaggccg gccggccgca
      481 gaatggcggc ctgcgccagg gccgctcgcg gggtgaatcc tccggctcgc tggatcacct
      541 cagagagttt ggtctgcccc gggtcgatga cgtagcttcc gggagacacc acttccccgc
      601 tgacccacac tgcgtactgc cggaggatct gtggattcgt tcggaccaaa atgacgtcgc
      661 ccggcacagc tgcccgatcg ggttgttccg cccggaggtc aatggtgaac gtctctggac
      721 catggggatt gctcaagcgg gtaatttcta cgcggctcga atcggcctgc tcggttaagc
      781 ccccggccag cgccaaaaga cataagagcg agtcatcttc ccggtactcg acggtgatgg
      841 ctgtgcccac ggctccggaa acggtgcagt cgccccggta gctgcgggcg ggcacgacaa
      901 tccggtcgcc ttcgcgcagc cgcgggttaa agcgggaatc ggaaatctgc tgcacccgaa
      961 acaggtcgca caaaaacctc tctccgtgac tgcgggtcaa ctcgattcgg cgcagtgaac
     1021 cgtcaccccg gataccaccg gctaaggcaa tagcttcggc gacccgcatg gccgccgaga
     1081 cctcgtaggt tcccggttta gccacccagc cgcccaccgg caccttgaag cgccgcattc
     1141 cgaccagggt catcgtcaca gtcgtgttcg aatagcgtcc ccgggcaaaa gctttcaact
     1201 cttctttgac gtgcgccagt gttttgcccc ccacggtaat ttcgcccagc gtcggaatca
     1261 aaagtgttcc ctcaggggtc accaccagcg attgggtctg ctcaaaacta ccccacactc
     1321 ccaaaaccag cacatcgccc ggtcccacca cataatcatt gggatcaata atctgatcga
     1381 gcgaggctga cggtgcggct cccaatcctc cagacggtgg tggcacctgg ctatgcgcgg
     1441 ataaactaac gacaacacag aagaaaaaga gtgcccgtct aaccactctc cgctcgcttg
     1501 ccgtttgagg ccaaaggaaa ggggcgtgca tactgctaac aggagaagta taaaccaaaa
     1561 tcgcagaaag tcaacttctg aatgggtcta ccgacagggc tccgatggcc atctccgtgc
     1621 cggctggtct agcccctccg gtttcctgaa ccggtctcgg cgacgccggt caagccggat
     1681 accactccac acccggcttg tgagcgagtg cttttccctc aattctcaat cctcagtcct
     1741 caatcctcac caccccgcac ctcagagccg aggtatcacg cctcggagaa caaagcggaa
     1801 ctcaaagtgc tcttactccc ggaacggctt cttcaggtaa cgttctaaac gccgccggtc
     1861 aaaaggaatc gacggcacgt ttaacccgaa ctggtacgcc gccaccgctt ctttccgctg
     1921 ctttttcaaa tcgtacgcct tccccagcgc cagtgcaatt ttgcccaagt agtagggacg
     1981 ttcttcgaca aattgagccg tctggaggtt cgcgatggcg gagtcgagga acccctttac
     2041 cagaaacatc tcgcccagta aatagtaagg gaacgagttg gaagggtctt cggtacgcat
     2101 attgcgcaag agagcccagg agttgttcaa agccaccgcc atttcattca aatcgccccg
     2161 gcgtttggcc acctgcccca gcgcaatgct cagttcggcc cggtctggat cggcaattct
     2221 tttccgcaga gcttcttcca gaagcggcac tcccagggat tcatctcccc gttcaacgta
     2281 aaaagacccc aaccgcacgt agggatcggc caggagactg tctccctgca tcgccgcccg
     2341 gtaatgcacc accgccgaat ccagctggtc ggcgaggagg aaaaaattgg ccgccacgac
     2401 ctggttctca gcggtcggat ggaaatggag attggttaaa gcgagcttga ggccctgcgg
     2461 gtactgcccc aggaggtagt gggtcagtac gagctggccg acctcatatt cgtcggtcga
     2521 atccagcgcc actaatttct ggaacagttc cagggcgtcc cggtagtggc cgaagcgcaa
     2581 ggcccgctcg gcaaagtggt tcaaatccgg tttgagcgcc gggaaggtat ccagataggc
     2641 ccgccattcc gcttccagcc cctccagcgg ttttccgtag accgccgtgc aggtgctgtc
     2701 cagactcaag tcggtcgcct gccggtagag ctgttccaag cgcgcccccc cgtaggtcgt
     2761 cgcgaggtag cgaacgaacg aagccgcctg gatcgtcgcc tccttggccg gctgagcata
     2821 gtactcctgg cttttcgaca gcgtggccaa cttcagcagt cgccccgccg ggaggtcctg
     2881 ttgcgcgtag tagtggctgt aatccaagta ccccgccagc ccttcggcca aaagggccgg
     2941 cgcatatccc cagaggtggc atatcttttc taaaataacc ggaaaaggat cgaccccgct
     3001 tttcccgggt tggtgaacgg cacagacacg ataccgcaga ggatccaccg caaagggaaa
     3061 gcgccggtcc cacacaagcg ccttggtttg acagggaaag tagtaaaatc ttacccgctc
     3121 cggcgtggtg agatcaaacg ttcgctgcaa ggcatccagt tcgtcctcca gattatcgcg
     3181 ccagcggttc cactggaaat cgcccaacga ccagaggcgg tagtagaaga tgaaacgggg
     3241 actcccttcg tagttccaga aggtcgagtc gagcggactg gtgtcgcact cgaaagtcgt
     3301 ctcggctggg gagggaacca aagtccactg caaggtggag cgccctttga gtggtagcga
     3361 cgccagggta agcgtctcac cccaattcaa agtgtcaatg cgcaggaaat gttccggctg
     3421 gcccccgaac gtgaaaagtt ccaactccag cccaatcatc ttcttggaaa taggtgtgac
     3481 ggtgagatcg aagatgaaat tccccgccag gccgttcacc cggacgttgt aaggacagac
     3541 aaattctcgc cggtcaatca ctcccgcctg cggtggattg cccctgtagt tttcgactaa
     3601 ctgactggaa aaccgaaccg ctttttccgc gtgtcctgga acaaccataa tgggtaaaca
     3661 caatagcagg aacgcgagaa tactgtttgc ctttctgtag gagcgcacct gcacttgtcc
     3721 tcttggtccc gagcacaatc gaggggagcc aagtcgcagg agatgcgccc gatccaagct
     3781 ccgcatcatt taatcaactt ccgcaatacc gccaagttga tcttgtcttc tttgtaatcc
     3841 ggccctttgg gcgtttccag aatcttggga atgtgggcca aacgcttatc gttcaagaaa
     3901 aagccgaacg cttctttccc caagaagcct ttgccaatgt gttcatgccg atccacccgg
     3961 ctgcccaagc ccttcttgga atcgttgaag tgtatcgcac ccaacctatc caggcccaca
     4021 gtgcggtcga aatcggcgat ggtctgttca tacccctttt ccgtccggat atcgtagccc
     4081 gctgcaaaga cgtggcaggt atccaggcag actgctagac gttccttctc tttggtaagg
     4141 tcaatcatgt cccgaagctg ttcgaactta aaccctaaat gcgccccctg accggcggtg
     4201 gtttccaaag cgatttttgc tttggccctt ggggtttgca catgcaccca gtccaacgcc
     4261 ccggcgatgt tcttgatgcc gatctcctcc cccgcgccta agtgcgagcc ggggtggagc
     4321 acgagaaagg gtatctggag cgcctcgcag cgctcgactt cgatagcgag cgcttcccgt
     4381 gacttctgat tcaaggcctt atccgatgag gcaatgttga tgaggta
//
LOCUS       DBNGZA010000220         4340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2691311, whole genome shotgun sequence.
ACCESSION   DBNGZA010000220 DBNGZA010000000
VERSION     DBNGZA010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2691311"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="AC16FU_04545"
     CDS             <1..656
                     /locus_tag="AC16FU_04545"
                     /inference="COORDINATES: protein motif:HMM:NF037740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cap15 family cyclic dinucleotide receptor
                     domain-containing protein"
                     /protein_id="HIR2993676.1"
                     /translation="PFIYFLAVLSVVVLFVAALIQGFDLGNFLDVLRLIPIVATVDVI
                     AYFVFAGWLWRWKRLQGWLIPFPDLNGTWHGNIQTNWKNADGNTPRPIPAILTIKQTF
                     GRMSCVMRTGEMESHSYLEGFCIDKDAQVRRLCYSYTSRPKAALRERSTPHDGTILFN
                     VVGNPVNKLEGEYWTQRQTAGTVTLTFRTKELVDEIPDDLPAHPITGGRGHIVNDKF"
     gene            733..2643
                     /gene="dnaK"
                     /locus_tag="AC16FU_04550"
     CDS             733..2643
                     /gene="dnaK"
                     /locus_tag="AC16FU_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR2993677.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGQPVVIANSEGMRTTPSVVAFTK
                     TGERLVGQVAKRQAITNPLNTIFSIKRFMGRKFREVTEEIKNFPYKVVEGPNGDARVQ
                     VEKEQYSPPEISAMVLGKMKQTAEDYLGEKITEAVITVPAYFNDAQRQATKDAGRIAG
                     LDVKRIINEPTAASLAYGLDKKKNEKIAVYDLGGGTFDISILELGDGVFEVRSTNGDT
                     HLGGDDFDKRIIDWLVAEFKKQEGIDLSRDPMALQRLKEAAEKGKMELSSTMETQINL
                     PFITADQTGPKHLNVTLTRAKMEQLVDDLIERTVEPCRKALADAKLTVDQIDEVVLVG
                     GMTRMPKVQQLVRDFFKKEPHKGVNPDEVVAVGAGIQGGVLVGDVKDIVLLDVTPLSL
                     GIETLGGVFTRLIERNTTIPTRKSEIFSTAENNQTKVEVHVLQGERQMAIDNKTIGRF
                     ILDGIPPAPRGIPQIEVTFDIDANGILHVTAKDKATGKEQKIRIEASSGLTEAEIKKM
                     VTDAKEHEGEDKKRREEVDTRNRADQLAYETEKNLKELEGKIEPDDKAKIEAAVGRVK
                     EALKGKNTEEIRSAQEALTQVWHQASAKIYQKTAGQSAPGGSTGGTEPPPGGDKGPEG
                     GQPVDADYEVVK"
     gene            2668..2973
                     /locus_tag="AC16FU_04555"
     CDS             2668..2973
                     /locus_tag="AC16FU_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR2993678.1"
                     /translation="MPTYEYRCTANHRFEEVQNISEPPIEKCPHCGAKAQRVISGGAG
                     LLFKGSGFYITDYRSESYKKKAKEESGESNPPKADSGGETKLSETKPEKPKKTEGSP"
     gene            3026..3448
                     /locus_tag="AC16FU_04560"
     CDS             3026..3448
                     /locus_tag="AC16FU_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993679.1"
                     /translation="MPLPLMRVPGKLSSRSILSGAATFWLGMALTIWLSACSKRPSEK
                     ELFTQAQMAVTEQRYYTGLDLYAQVLRNFPKSENAYKALFMLGYVNSEFLHRPTVARD
                     YFKKVLEKYPGCDLADDAVYLLDNQSGLARPLPNPTTP"
     gene            3550..4026
                     /gene="dtd"
                     /locus_tag="AC16FU_04565"
     CDS             3550..4026
                     /gene="dtd"
                     /locus_tag="AC16FU_04565"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR2993680.1"
                     /translation="MRLVLQRVSRARVTVGGNVTGEIGPGLLILLGAKRDDTGKQADF
                     LADKVLGLRIFEDQAGKMNLSVRDVRGEILVVSQFTLYADTRRGRRPSFTEALEPQQA
                     EELYGHFVNRLKASGLVVATGSFGAKMEVELVNAGPVTLVLEKEEGKAEAEAERSA"
     gene            4044..>4340
                     /locus_tag="AC16FU_04570"
     CDS             4044..>4340
                     /locus_tag="AC16FU_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR2993681.1"
                     /translation="MNFVRPQTYTRFAEKIKLLLRGDRLILASSSPRRAQMLAELGLS
                     FFALRPRLVEEDLVIACPKARSVDLSGRKAASVLPEASAGLVLGADTSVVRGGEI"
BASE COUNT         1067 a         1198 c         1258 g          817 t
ORIGIN      
        1 agccgtttat ttattttctg gctgtgttgt ctgtcgtagt cttatttgtc gccgccttaa
       61 ttcagggctt cgaccttggt aacttcctcg acgttcttag acttatacct attgttgcca
      121 cggtggacgt tatcgcctac ttcgtcttcg ccggttggct ttggcgctgg aagcgtctac
      181 agggctggct catcccgttc cccgacctga acggcacatg gcacggaaac attcaaacga
      241 actggaaaaa tgccgacgga aacacgccca gacctatccc cgctatcttg actatcaagc
      301 agaccttcgg gcgtatgagt tgtgtcatga gaactggcga gatggaaagc cacagctacc
      361 ttgaggggtt ctgcatcgac aaggacgctc aagttcgacg gctttgctac tcctacacaa
      421 gcagaccgaa ggccgctttg cgtgaaagga gcacgccgca tgacggcacg atacttttca
      481 acgttgttgg aaatccagtt aacaaactcg aaggcgaata ctggacacaa cgtcaaacag
      541 ccggaaccgt aacactaacc tttcgaacga aggagcttgt tgacgaaatc ccggacgacc
      601 ttccagcgca cccgatcaca ggtggcagag gtcatatagt aaatgataag ttttgacgtt
      661 aaaacacagt gcgggaaacc acaaaaaaac atagcggagc gcgaatgcgc ccgcgataag
      721 gagtgacaaa acatgggtaa aatcattggc attgacttgg gaaccaccaa ctcgtgcgtg
      781 gcggtgatgg aaggcggcca gccggtcgtc attgccaatt cggaaggcat gcgcaccacg
      841 ccttcggtcg tggcttttac caaaaccgga gagcgtctcg tgggccaggt ggccaaacgc
      901 caggccatca ccaatcccct gaacacgatt ttctccatca agcggttcat gggacggaaa
      961 ttccgcgagg tgaccgagga aatcaaaaac ttcccctaca aagtggtcga agggcccaac
     1021 ggcgacgccc gggtgcaggt ggagaaagag caatactctc cgcccgaaat ctcggccatg
     1081 gttttgggaa aaatgaagca gaccgccgag gattacctgg gtgaaaaaat caccgaagca
     1141 gtaatcaccg tcccggccta tttcaacgac gcccagcgcc aggcgaccaa agacgccggc
     1201 cgcatcgcgg ggctggacgt caaacgcatt atcaatgagc cgaccgcggc ttcgctcgct
     1261 tacggtttgg ataaaaagaa aaacgaaaag atcgccgtct acgacctggg cggcggcacg
     1321 ttcgacattt cgattctcga attgggcgac ggcgtcttcg aggttcgctc gaccaacggc
     1381 gacacccact taggcggcga tgactttgac aagcggatca tcgactggct cgtggccgaa
     1441 ttcaaaaagc aggaggggat cgatctctcg cgcgacccga tggcgctcca gcgtctcaaa
     1501 gaagcggcgg agaaggggaa gatggaactc tcctccacga tggaaaccca gatcaacctc
     1561 cccttcatca ccgccgacca gaccggcccc aaacacttga acgtcacgct cacccgcgcc
     1621 aagatggagc agttggtcga tgatttaatt gagcgcacgg tggagccgtg ccgcaaggcc
     1681 ttagccgatg ccaaactaac cgttgaccaa atcgacgagg tcgtgttggt gggcgggatg
     1741 acccggatgc cgaaggtcca gcagttagtc cgcgacttct tcaaaaaaga gccgcacaag
     1801 ggagtcaatc cggacgaagt ggtggcggtc ggcgccggca ttcagggcgg cgttttagtc
     1861 ggcgatgtca aggatatcgt cctcttggac gtgacgcccc tttcgctcgg tatcgaaact
     1921 ttaggcgggg ttttcacccg gctgatcgag cggaacacca ccatcccgac ccgtaaatcg
     1981 gaaatcttct ccaccgcgga gaacaatcag acgaaggtgg aagtccacgt gctccagggc
     2041 gaacggcaga tggcgataga caacaaaacc atcggccgct tcattctgga cggcattccg
     2101 ccggcgccgc gcggcatccc gcagatcgaa gtcaccttcg acatcgacgc caacggcatc
     2161 ctgcacgtca ccgccaaaga caaagccacg ggcaaagagc agaaaatccg gatcgaggcc
     2221 tccagcggtc tcaccgaggc ggaaatcaag aaaatggtca cggacgccaa agagcacgaa
     2281 ggcgaagata agaagcgccg cgaagaagtc gatacccgca accgggccga ccaactggcc
     2341 tacgaaaccg aaaagaatct gaaagaattg gaaggtaaaa ttgaaccgga cgacaaggcc
     2401 aaaatcgaag cggcggtggg ccgggttaaa gaagcgctca aggggaaaaa cactgaagaa
     2461 atccgctcgg cccaggaagc cctcacccag gtatggcacc aggcctcggc caaaatctac
     2521 cagaagaccg ccggtcagtc cgccccaggc ggatcaaccg gtggaacgga acccccgccc
     2581 ggcggcgaca aagggccgga gggcggccaa cctgtcgatg ccgactacga ggtcgtgaag
     2641 taagacggcg gctaagtaca gatcgctatg ccgacgtacg aataccgctg caccgccaat
     2701 caccggttcg aggaggttca gaacatctcc gagccgccga ttgagaagtg cccccactgc
     2761 ggcgccaaag cccagcgtgt gatctccggc ggtgccgggc tgctcttcaa gggttccggt
     2821 ttttacatca ccgactaccg gagcgagtcg tacaaaaaga aagccaagga ggagtcgggc
     2881 gaaagtaatc cgcctaaggc ggactcaggc ggtgagacca agttgtcgga aacgaagccg
     2941 gagaaaccaa agaaaacaga gggtagtccc tgaacgccgc cggtaaggga agttactctt
     3001 ctggggccga aatttcagca aaccgatgcc gttgccattg atgcgcgtgc cgggcaaatt
     3061 gtcttcacgt agcattcttt ccggggccgc gactttttgg ctggggatgg ccctgacgat
     3121 ttggctcagc gcttgttcca aaaggccttc cgaaaaggaa ctctttactc aggcgcaaat
     3181 ggcggtaacg gagcagcgct actacacggg acttgacctc tacgcgcagg tcttgcggaa
     3241 ttttccgaaa agcgaaaacg cctacaaagc gctcttcatg ctggggtatg tcaactccga
     3301 attcttgcac cgcccgactg tggcgcgcga ctatttcaaa aaggtgttgg aaaaatatcc
     3361 cggctgcgat ttggctgacg atgccgttta tttactcgac aatcagtccg gtttagcccg
     3421 accattgccg aatcccacca ccccctgagt taactttttg accggccaca agatatagtg
     3481 gcgggttcaa agatttctct tgacaaattt ttgccaggcg gctatcctat cacctgatca
     3541 aggaggtgtg tgcgccttgt gctccagagg gttagccggg ccagagtgac cgtcggcggc
     3601 aatgtgacgg gcgaaatcgg cccgggactg ctcatcctct tgggagccaa aagagacgac
     3661 accgggaaac aggccgactt cctcgcggac aaagttctgg gcctgcggat ttttgaagac
     3721 caggcgggga agatgaatct ttcggttcgg gacgtcagag gagaaattct ggttgtctcg
     3781 caatttacgc tgtatgcgga cacccgcaga gggagacggc ccagtttcac cgaggcgttg
     3841 gaaccgcaac aagcggagga actgtacggc cattttgtga accggctcaa agccagcggg
     3901 ctcgttgtcg ccaccggctc gttcggcgcg aagatggaag tggaactggt caatgccggg
     3961 ccggttacac tggtactgga gaaggaggag gggaaagcgg aggcagaggc agagcggtcc
     4021 gcctgaggcg ggcagggaag ttgatgaatt ttgtccggcc gcaaacctat acgcgttttg
     4081 cggaaaaaat taagctcctt ctgcgcggcg atcggctcat tctggcatcc agttcgccgc
     4141 ggcgagctca gatgttggct gagttgggcc tttctttttt cgcgctccgc ccccggttgg
     4201 ttgaggagga tctggtgatc gcctgtccca aagcccgctc ggttgatctc tcgggtcgca
     4261 aagccgcttc ggtactgccc gaagcgtccg ccgggttggt actgggcgcg gatacgtcgg
     4321 tcgtacgcgg aggcgaaatt
//
LOCUS       DBNGZA010000221         4954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2691384, whole genome shotgun sequence.
ACCESSION   DBNGZA010000221 DBNGZA010000000
VERSION     DBNGZA010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2691384"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="AC16FU_04575"
     CDS             complement(<1..601)
                     /locus_tag="AC16FU_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2993682.1"
                     /translation="MKKIAVITSKGGTGKTTTAVNLAHGLALSGKKVLVIDCDSQGSV
                     AFAFGVQAERSLAELLVRGEVDFVRARPGLFVVDSGGKKLAEAELYLAARDDREVRLA
                     LALESFAGADYVLCDCSPSINLMNLNALFFCDSMLIPVSLDTFALKGVEKMLALAADV
                     RQAGHPIEPLGILATFYDGRTNIAKRLWNELLECYGEKVL"
     gene            complement(598..3465)
                     /locus_tag="AC16FU_04580"
     CDS             complement(598..3465)
                     /locus_tag="AC16FU_04580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2993683.1"
                     /translation="MSIFQKPIKEIYATFPAEESRLEEVRTFVQTVTQDTGLASKDLT
                     GIQLAIEEACTNVIRHAYLYGPGTIRLKIVLGKDAIVFSIYDTGRQFNFGQAAGATPD
                     LKRYIATGRKGGLGLYLIQKIMDDVSYRSHNGTNEMRLTKKIPRKVPLSMRPVRGLSV
                     RVKFSLLSAAIVLAIMAAVFFFFDHRTTTAIYRADLANVEQLARTVAAAAEDPLLSGS
                     DLELMTVASGTRRQHPELSYLVVVDTGGVVLADPDRVEQVLTAYPLPPGLAPRRFWTP
                     QVVSPAGQETYHTVAPISIGGTFRGFVHVGLAKKTIEAEVVRARYVLILICLVGLLVG
                     FLAIWTLAQWFVKPIQKLTEGVLRIGQGELDATLPVEGKDEFGEIAKAFNDIALKFKQ
                     SQKTLLQQERLQKEIEVAQEIQRALLPRQFPEVEGYDIATIYRAAKDVGGDYYDFFWI
                     DEKTMGIVVADVSGKGVPGSLVMTMIRTAIRLEARGNRSPVEILSRVNAFVADDVKKG
                     MFITIFLVVLDSVNRTITYTSAGHNPMILYRKEEDRAYFLNPRGIPLGISLPDDLSFE
                     ETLAAETVRLKKDDILVIYTDGITEAMNAQKEQFGTQRLLDFIRANCPLAAEEFAEKF
                     NDELSTFTGDLPQHDDITLVAVKEQLVADKVVFATRKRLLDLVAVDGKTVREACAVIG
                     VAPTTYYRYKRLYDQFGPEGLKARTPRLGRGEEMRQVSIDDRQKILSVVALHPEYGPA
                     RVKSELASERFGHLTLDEEVIYDELVRLRLNTKLLRLEFAERTKGQLPATQAHLIDLE
                     RQKEKEQGEAERAAAERIAAAQRQEAAAQQAERQETFLTQLREQGLEPDDLALLAMVA
                     GELKQVAEDEKVEKLFRLIGEKIVAEKKKKAGISGSPRPKVLDWQRLVTSHKPVTQKP
                     EAVTVPEEPGEEKPFNWEEYSRRVKARLVRSPRVPDEGKPE"
     gene            complement(3613..>4954)
                     /locus_tag="AC16FU_04585"
     CDS             complement(3613..>4954)
                     /locus_tag="AC16FU_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993684.1"
                     /translation="SDVGFGADVGLLLPRWRGFGFGLTWQDAIGARLTLNQSREIMPW
                     NLKGGLSYERFVAADRVGLLFSADVDQVQGGQARVRVGGEVAIRRQLFLRLGLDKDEI
                     TFGAGVGWRSFAFDYAYQSVLDLEASHRFSLSIAFGSSRAERLATHNKAAEEKALRVY
                     QESQKEKVQNLFERAQTFDKAGAVDSALFFYQQVLAYDAENARARERMAALKEQVKSQ
                     AEQAVQARSQELIVTDHLRLAEQYQGEGNLALAQAELQQVLAQSPQNPEALRRQKEIA
                     GQIGRQKMAFDSTAEVFFTRKDYSGAIGEWRKALVLNPGDTLFERKIKRAEERLSAAQ
                     LLQEGLAKYTAGEYSAALAIFGNVLRVDPQNPVAREYQKLSQQKMAKVTTLEDLKKDP
                     QAWQWYLAGLESFQKGDYEKAIELWNQVLAVYPNNQNALRNIEQAKKRLPKSGQ"
BASE COUNT          922 a         1591 c         1359 g         1082 t
ORIGIN      
        1 ccagcacttt ttcgccgtag cactcgagca attcgttcca caggcgtttg gcgatgttcg
       61 tccggccgtc gtagaaggtg gccaaaatgc ccaagggctc gatgggatgc cccgcctgcc
      121 gcacgtcggc ggccaaagcg agcatctttt cgaccccttt cagggcaaag gtgtccagac
      181 tcaccggaat gagcatcgag tcgcagaaaa aaagggcatt caggttcatc aagttgatcg
      241 acggcgaaca gtcacacagg acgtaatccg ctccggcgaa ggactccagc gccaaagcca
      301 gccgcacctc ccggtcatcc cgcgcggcta agtacagttc cgcctcggcc aatttcttgc
      361 cgcccgagtc gacaacgaag agaccgggcc gcgcccggac gaagtccacc tcgcctctta
      421 ccagtaattc ggccaaagaa cgctccgcct gaaccccaaa ggcgaaggcc acgctcccct
      481 gcgagtcgca gtcgatcacc aaaacctttt ttccggaaag ggccagcccg tgcgccagat
      541 tgacggcggt ggtggttttg cccgtgcctc ccttggaggt gatgacggcg atttttttca
      601 ttccggcttt ccctcgtccg gtacccgcgg cgaccgtacg agccgcgcct tcacccgccg
      661 gctgtactcc tcccaattga acggtttttc ctctcccggt tcttcgggta cggtcaccgc
      721 ttcgggcttt tgggtaaccg gtttgtggct ggtcaccagc cgctgccaat ccaacacctt
      781 gggccgcggg ctgccagaaa tgccggcctt tttcttcttt tcggccacaa tcttttcgcc
      841 tatcaagcgg aagagctttt cgactttttc atcctcggcc acctgcttga gttcgcccgc
      901 gaccatcgcc agaagtgcca gatcgtcagg ctccagtccc tgctctcgga gctgggtgag
      961 aaatgtttcc tgccgctcag cttgttgcgc cgccgcctcc tgtcgctggg ctgccgcaat
     1021 gcgctcagct gccgcccgct cggcctcgcc ctgttctttc tctttttgcc gctccaggtc
     1081 tataaggtga gcctgggttg ctgggagttg tcctttcgtc cgctccgcga actccaaccg
     1141 caacagtttc gtgttcagcc gcaggcggac caattcgtcg taaatcacct cttcgtccag
     1201 cgtcagatgg ccaaaccgtt cgctggccag ctcgctcttg acccgggccg ggccgtactc
     1261 cgggtggagg gcaacgacgg agagaatctt ctgtcggtca tctatggaaa cctgccgcat
     1321 ctcctcgccc cgccctaggc ggggcgttcg ggctttgagg ccctccgggc cgaactggtc
     1381 gtacagtctt ttgtaacggt aataggtagt cggcgccacc cctatgacgg cacaggcttc
     1441 ccgaactgtt ttcccgtcga cagccaccag atcgagcaga cgcttgcgcg tggcgaagac
     1501 caccttgtcg gccactaatt gctctttcac cgccaccagg gtaatgtcgt cgtgctgggg
     1561 cagatcgccg gtgaaggtgg aaagttcgtc gttgaacttc tcggcgaatt cttccgccgc
     1621 gagggggcag ttggcccgga tgaaatccaa aaggcgttgg gtcccgaact gctctttctg
     1681 cgcattcatc gcctcggtga tcccgtcggt gtagataacc aggatgtcgt cctttttcag
     1741 ccgcaccgtc tcagccgcca gcgtttcttc gaacgacaga tcgtcgggca gcgagatgcc
     1801 cagggggatg ccgcgggggt tcaaaaaata agcccggtct tcctccttgc ggtagaggat
     1861 catcgggttg tgcccggccg aggtgtaagt gatggtccgg ttcaccgagt cgagcaccac
     1921 caaaaaaatg gtgatgaaca tccctttttt gacgtcgtcg gccacgaacg cgttcacccg
     1981 ggagaggatt tccacgggcg accggttgcc gcgcgcttcc agccggatgg cggtgcggat
     2041 catggtcatg accaaggacc cgggcacccc tttgcccgaa acgtcggcca ccacgatccc
     2101 catggtcttc tcgtcgatcc agaagaagtc gtagtagtcg ccccccacgt ccttggccgc
     2161 ccggtagatg gtggcaatgt cgtacccctc cacttccggg aactggcggg gcaaaagcgc
     2221 gcgctggatc tcctgcgcca cctcgatttc cttctgcagc cgctcttgct gcaaaagcgt
     2281 cttctgactc tgcttgaatt tcaacgcaat gtcgttgaag gccttggcga tttcgccgaa
     2341 ttcgtctttc ccttccaccg gcagggtggc atctagttcg ccctgtccga tccgcaatac
     2401 tccctcggtc agtttctgaa tcggcttgac aaaccactgc gctaaagtcc agatggccag
     2461 aaacccgacc agaagcccca cgagacagat cagaatcagg acgtaacgcg ctcgcacgac
     2521 ctccgcctcg atggtctttt tggccaaacc gacatgcacg aagccccgaa aggtaccgcc
     2581 gatgctgatg ggagcaaccg tatggtacgt ttcttggccc gctggggaca cgacctgcgg
     2641 cgtccagaag cgccgcggcg ccaggcccgg aggcaacggg tatgcggtca ggacctgttc
     2701 cacccgatcg ggatcggcca ggaccacccc gccggtgtcg accaccacca gataggaaag
     2761 ctccggatgc tgccgtcggg tgccggaggc caccgtcatc aattccaaat cgctgcccga
     2821 caagagcgga tcttccgccg ccgctgccac cgtccgggcc aactgctcca cgttcgccag
     2881 gtcggcacgg taaatggcgg tggtggtccg gtgatcgaag aaaaagaaaa ctgcggccat
     2941 gatggcgagc acgatggcgg cggacagaag cgaaaacttc acccgcaccg acagtccgcg
     3001 caccggtctc atcgacagcg gcaccttcct gggtattttt ttggtcagcc gcatctcgtt
     3061 ggtgccgttg tgcgaccggt acgacacgtc gtccataatc ttctggatca ggtagagccc
     3121 cagcccgcct ttgcgcccgg tggcgatgta gcgtttcaga tccggtgtcg cccccgccgc
     3181 ctggccaaaa ttgaactgcc gcccggtgtc atagatcgaa aagacgatgg cgtctttgcc
     3241 caggacgatt ttgagccgga tggtccccgg cccgtacagg taggcgtgcc ggatgacatt
     3301 ggtgcaggcc tcttcgatgg ccaattgaat gccggtgagg tctttggaag ccagaccggt
     3361 gtcctgcgtc accgtttgga cgaaggtgcg cacttcctcc aggcggcttt cctctgccgg
     3421 aaaagtggcg taaatctctt tgatcggctt ttgaaaaatg ctcatcgtgt ttttattcca
     3481 acactttgat ggtttcgctt actttgaccc gtcaaaaaaa tgagtcatcc gcgtggggcg
     3541 acccctacgg gccgtatggc cgcctcggtc gcccttcttc gattacctca ctggaacaac
     3601 tcgccgcctc tctcactgcc ccgacttcgg caaccttttt ttcgcctgct caatattgcg
     3661 cagcgcattc tgattattgg gataaacggc caaaacctga ttccacaact caatcgcttt
     3721 ctcgtaatcc cctttctgga aactttccag ccccgccaga taccattgcc aggcctgcgg
     3781 atctttcttt aagtcctcta aggtggtcac tttggccatc ttctgctggg agagcttctg
     3841 gtattctctg gcgaccgggt tctgcggatc tacccgcaag acgttcccga agattgccag
     3901 agccgctgag tactccccgg ccgtgtactt cgctaagccc tcctggagca actgggctgc
     3961 ggacagccgc tcctccgccc gcttgatttt ccgctcgaaa agcgtatcac ccggattgag
     4021 cacgagggct ttgcgccatt cgccgattgc gccactgtag tcttttcggg tgaaaaaaac
     4081 ttcggcggtg gaatcgaacg ccatcttctg cctgccaatc tgtcccgcta tttccttttg
     4141 tcggcgcagg gcttcgggat tttgaggcga ctgggccagc acctgttgga gctccgcctg
     4201 tgccaaggcc aggttcccct caccctggta ctgctctgcc agccgcaggt ggtcagtcac
     4261 tatcaactcc tgcgagcggg cctgtaccgc ctgctcggcc tgggatttga cctgttcctt
     4321 gagcgccgcc atccgttcgc gggctcgggc gttctccgcg tcgtacgcca gaacctgctg
     4381 gtagaagaag agggccgagt ccaccgcgcc ggccttatcg aacgtctgtg ctcgctcgaa
     4441 gagattttgc actttctctt tttgactctc ctgatatacc cgcagggctt tttcttcggc
     4501 ggctttatta tgggtggcca aacgttctgc tcggctggaa ccgaacgcta tcgaaaggga
     4561 gaaccggtgc gacgcttcta agtccaaaac actctggtac gcgtagtcga aagcgaatga
     4621 ccgccagccg acgccggccc cgaaggtgat ttcatctttg tctaaaccga gccggaggaa
     4681 caattgccgg cggatcgcca cttctccacc aacgcgcacc cgcgcctgcc cgccctgcac
     4741 ctgatccacg tcggcggaaa agagcaaacc gacccgatcc gcagcgacaa aacgctcgta
     4801 actgagtccg cccttcagat tccagggcat aatttcccgt gactggttca gggtcaaacg
     4861 ggcgccgatt gcatcctgcc aggttaaacc gaacccgaat ccccgccagc gcggcaggag
     4921 caaaccgaca tcggcaccga aacccacgtc actc
//
LOCUS       DBNGZA010000222         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_42754, whole genome shotgun sequence.
ACCESSION   DBNGZA010000222 DBNGZA010000000
VERSION     DBNGZA010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_42754"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /gene="fusA"
                     /locus_tag="AC16FU_04590"
     CDS             <1..1416
                     /gene="fusA"
                     /locus_tag="AC16FU_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR2993685.1"
                     /translation="DDHLLAKFVHDEPIAHEEIAAALRRATLDGKIIPVLCGSSFKNK
                     GVQKLLDAIVDFLPSPLDMPPLVGAAMSNGNEIVRRPDDREPLTALAFKIMSDPYVWK
                     LTFFRVYAGRLASGSYVLNANTGQKERIGRLLLMHANKREEVEEVYAGDIAAAVGLRK
                     TSTGNTLCDPDHPILLEKMVFPDPVIFVAIEPKTKADQEKLSEALARLADEDPTFKVR
                     TDEETGQTVISGMGELHLEILVDRMLREFKVQANVGRPQVAYKETITKAVEAEGKFIR
                     QTGGRGQYGHVVIDLEPLPPGKGFVFENKVIGGTVPREYVPAVERGIRDALENGVLAG
                     YPLVDLKAVLTDGSYHDVDSSEIAFKIAGSMALQDGAKKAGPKLLEPVMNVEVVVPET
                     YLGDVMGDLQARRGKIGGMFPRADGQVVAATVPLAEMFGYATKLRSLSQGRAVFTMQF
                     AHYEEVPKSVAEEIVAKVRGV"
     gene            1622..1930
                     /gene="rpsJ"
                     /locus_tag="AC16FU_04595"
     CDS             1622..1930
                     /gene="rpsJ"
                     /locus_tag="AC16FU_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR2993686.1"
                     /translation="MPGQKIRIRLKAYDHHSLDQSTKEIARTVLRTGAKLVGPIPLPT
                     ARSVYTVLRSPHVDKNSREQFETRIHKRLIDIYDSTPQTVDALIKLELPAGVDVEIKV
                     "
     gene            1954..2586
                     /gene="rplC"
                     /locus_tag="AC16FU_04600"
     CDS             1954..2586
                     /gene="rplC"
                     /locus_tag="AC16FU_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR2993687.1"
                     /translation="MPGAILGRKIGMTQIFNAAGVAVPVTVVEAGPCPVVQVKTAAKD
                     GYGAVQLGFGARRKNLFTRPLAGHFARHKVEPTHLLREVSLRPDEKIEPGQAVTVELF
                     QVGETVNVIGVSRGHGFQGTVKRHHFKGGPKTHGQSDRWRAPGSIGASSFPSRVLKNV
                     RMAGRMGGNRVTVRNLSVVGVDAERHLLLISGSVPGKPRGWLLVSKVPKR"
     gene            2622..>3242
                     /gene="rplD"
                     /locus_tag="AC16FU_04605"
     CDS             2622..>3242
                     /gene="rplD"
                     /locus_tag="AC16FU_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR2993688.1"
                     /translation="MIVAKVYNSAGEEKEKTALSAALFGVTPHRPVLYQYVKMYLGNQ
                     RQGTHATKTRAEVSGGGRKPWRQKGTGRARSGSNTSPVWVRGGSAHGPKPRDYETKMP
                     QQARRLALKSALSAKAAAGKIVLFEPPSLEKPKTKILVAYFSKIGVLPGRTLVLAEGK
                     DERLGLSCRNIPGVTFKRAAAVNAYDLWIADSVLLTKPALAAVEEVF"
BASE COUNT          702 a          944 c         1023 g          573 t
ORIGIN      
        1 gacgatcacc tcctggccaa gttcgtccac gacgagccga ttgcccacga ggaaatcgct
       61 gcggctttgc ggcgggccac cctcgacggc aaaatcattc cggtcctctg cggctcctcg
      121 ttcaaaaaca agggcgtgca gaaactctta gacgccattg tcgacttcct cccctcgcct
      181 ctggatatgc cgcctctggt cggtgcggcc atgtcgaacg ggaacgagat cgtgcgccgc
      241 cccgacgatc gggagccttt gaccgctctg gcgttcaaga tcatgtccga cccctacgtg
      301 tggaagctga ccttcttccg ggtctacgcc ggacggttgg cctccggctc ctacgtgctc
      361 aatgccaata ccggccagaa agaacggatc ggacggctgc ttttgatgca cgccaacaaa
      421 agggaagaag tcgaagaagt gtacgccggc gatatcgccg ccgccgtcgg tctgcgcaaa
      481 acttccaccg gcaataccct ctgcgatccc gaccacccga ttcttctgga aaagatggtt
      541 tttcccgatc cggtgatttt tgtcgccatc gaacccaaaa ccaaagccga ccaggaaaaa
      601 ctctccgaag cgctggcccg cctggcggat gaggacccca ccttcaaagt ccgtaccgac
      661 gaggagaccg gccaaacggt catctcgggt atgggcgaac tgcatctgga gatactggtc
      721 gaccggatgc tccgggaatt caaagtccag gccaacgtgg gccggccgca ggtggcctac
      781 aaggaaacca tcacgaaggc ggtggaagcc gaaggaaaat ttatccggca aaccggcggc
      841 cgcgggcagt acggccacgt cgtcatcgac ctggaaccgc tcccaccggg aaaaggtttc
      901 gtcttcgaga acaaagtcat cggcggcacg gtgccgcgcg agtacgttcc cgccgtggag
      961 cgcgggattc gcgacgcact ggagaacggc gttctggccg gctacccctt ggtggacctg
     1021 aaggcggtgc tcaccgacgg gtcgtaccac gacgttgatt cgtcggaaat tgcttttaaa
     1081 atcgccgggt cgatggccct gcaagacggg gccaagaaag ccgggccgaa gctcttagag
     1141 ccggtgatga acgtcgaagt ggtggtcccc gaaacctatt taggcgatgt gatgggcgac
     1201 ctccaggcgc ggcgcgggaa gatcggcggg atgttccccc gggccgacgg ccaggtggtg
     1261 gccgccaccg tgcctctggc ggaaatgttc ggctacgcca ccaaattgcg ctccctctct
     1321 cagggccggg cggtcttcac catgcagttc gcccactatg aggaagtgcc caaatcggtg
     1381 gccgaagaaa ttgtggcgaa ggtgcggggg gtttaagacc gcaaaaaagc acttgcgctt
     1441 ttttctcttt ctcatacatt aaaggtttcc cttgccaagc acggtaggga taaaaaggga
     1501 aatagtcttt tttgacggag atatacggtt cggtgagcgg cgggcagaag tccccgcgcg
     1561 ggaacggatg aactcaggag cgcggggaat cgaatcccgc gcgctgagaa gagaaagcga
     1621 aatgccggga cagaaaatca ggatcaggct caaggcgtac gatcaccact ccttggacca
     1681 gtcaaccaaa gagatcgcgc gcacggtgct gcgcaccggc gcgaaattgg tcggtcccat
     1741 cccgttgccg accgcacgct cggtctacac cgttttgcgg tcgccgcacg tggacaagaa
     1801 ctcgcgcgag cagttcgaga cgcggattca caagcgtctg atcgacatct acgactcgac
     1861 gccgcagacc gtcgacgcct tgatcaagct ggaactgccg gccggcgtgg atgtggagat
     1921 caaagtttag tttttcggag acagagagcg aaaatgccgg gtgcgatttt aggcagaaaa
     1981 attggaatga cacaaatctt caacgccgcc ggtgttgcgg tgccggtgac ggtggtcgag
     2041 gccggcccct gtccggtcgt gcaggtcaaa accgcggcca aggacgggta cggggcggtg
     2101 cagctggggt tcggcgcacg gcggaaaaac cttttcacca ggcctttggc gggtcatttc
     2161 gcgcggcata aagtggaacc gactcatctc ttgcgggaag tgtccctgcg gcccgatgaa
     2221 aaaatcgaac ccggccaggc ggtgaccgtc gagctctttc aagtcggcga gacggtgaac
     2281 gtcattggcg tttcacgcgg tcacggcttt cagggaacgg tcaagcggca ccacttcaaa
     2341 ggcggaccca aaacgcacgg ccagtccgac cgctggcggg cgccgggatc gatcggggcc
     2401 agttccttcc cctcgcgggt gttgaaaaac gtgcggatgg cgggacggat gggcggcaac
     2461 cgggtgaccg tacggaatct ttccgtagtc ggcgtagatg cggaaaggca cctccttttg
     2521 atttccggat cggtgccggg gaagccccgg ggctggctct tggtcagcaa ggtgccgaag
     2581 cgctaagacc ggagtcggga attcccggag aaactgaaat gatgatcgta gccaaagtct
     2641 acaatagtgc gggagaggaa aaagaaaaga cggcgttgag cgcggcgctc tttggggtca
     2701 cgccgcaccg gccggtgttg taccagtacg tcaaaatgta cctggggaac caaaggcagg
     2761 ggacgcacgc caccaagacc cgcgccgaag tttcgggcgg ggggcgcaaa ccgtggcgcc
     2821 agaaggggac cgggcgcgcg cgctccggca gtaacacctc accggtctgg gtgcgcggcg
     2881 ggagcgccca cggccccaag ccgcgcgact acgaaaccaa aatgccgcag caggcccggc
     2941 ggctggccct caagtcggct ctttccgcaa aagctgcggc cgggaaaata gtcctctttg
     3001 aaccacccag tttggaaaaa ccgaaaacga aaatactggt tgcctacttc tcaaagattg
     3061 gggtgcttcc cgggcggacc ctggtgctgg cggaagggaa agacgaaagg ctcggacttt
     3121 cgtgccggaa cattcccggt gtcacgttca agcgggccgc cgccgtcaac gcctacgacc
     3181 tgtggatcgc cgactccgtc cttttgacca aaccggccct ggcggccgtt gaggaggttt
     3241 tt
//
LOCUS       DBNGZA010000223        11865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2480295, whole genome shotgun sequence.
ACCESSION   DBNGZA010000223 DBNGZA010000000
VERSION     DBNGZA010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2480295"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..646)
                     /locus_tag="AC16FU_04610"
     CDS             complement(104..646)
                     /locus_tag="AC16FU_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR2993689.1"
                     /translation="MLLGVVLERKVLRNKMAMNPCNCGFFGDPGHECSCTPTQIQKYM
                     SRISGPLLDRIDIHIEVPAVKFRELAGQPTGESSAAIRERVNRARQVQLERFKSEKSI
                     YCNAHMQSKEIRKYCLIDDKSQELLRLAMQKLGLSARAYDRILKVSRTIADLEGSVEI
                     QPHHVSEAVQYRSLDRNLWL"
     gene            658..1053
                     /locus_tag="AC16FU_04615"
     CDS             658..1053
                     /locus_tag="AC16FU_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2993690.1"
                     /translation="MSTIGDHIRKRRLDLGLWQRQVAQQIGVDESTITNWEKHRNFPS
                     LRFMPRIIQFLGYDPCPAEADQPLPARLKTCRKGLGLSQKRLAKILRVDPTTVASWEA
                     ATNRPTAKSIKLIDSFLTSQTLAALVSSE"
     gene            complement(1457..2356)
                     /locus_tag="AC16FU_04620"
     CDS             complement(1457..2356)
                     /locus_tag="AC16FU_04620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR2993691.1"
                     /translation="MWVYIIGYFSLLALLYYREHFSPTEPLLVLLVVGGLFSLLAVWL
                     TRGRKAHSFPVNHPRAESRLLVGYLFVIVAFLTWGIPFLRTLGSTQFSSSLALLTGKL
                     LVFVVVPLILWKFLWGYTPACLLGLPIRLKGHWRPVLGMAGVLIVFQLVFGRALKALA
                     ETAHSPVVLGIASFLAFGWLLVEVGLVEEFFFRGLLQTRLAVWTGSECTAIVLVAVLF
                     GLAHAPGLYLRPETTGEALGTAPSLIKAVGYAITVTSVTGFFLGVLWARLRNLAALII
                     IHTAGDFFPNLVDFVRMVSQHSR"
     gene            3193..4380
                     /locus_tag="AC16FU_04625"
     CDS             3193..4380
                     /locus_tag="AC16FU_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2993692.1"
                     /translation="MSSALRFDIRRGAGLAALLILSFSFSSASGQFTLLYQKNGAAAS
                     DFFGRSVSSAGDVNGDGKADFIIGAYNTAPRDINGIGAGSAYVYSGVDGALLFQRNGA
                     AAGDLLGYSVASAGDVDGDGKADFIIGAPQAHVGSFINNIGSAYVYSGATGALLYQKD
                     GATTGDLFGTSVASSGDLNRDGKSDFIVGANNAQPGGLTSAGSAFVYSGATGALLYQK
                     NGAAAFDNCGYSVASAGDVNGDGKPDFIIGANVADPGGLTSAGSAYLYSGTTGALLLQ
                     KDGATTGDQLGISVASAGDVNGDGRADFIIGAHAASPGGLFNAGSAYVYSLPCVVRGD
                     VTNDAATDIVDIVALVQNVVFGTPLPNPLAGDVNCDTVRDVTDIVSLIQYVVFGTPTP
                     CCL"
     gene            4395..10247
                     /locus_tag="AC16FU_04630"
     CDS             4395..10247
                     /locus_tag="AC16FU_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2993693.1"
                     /translation="MTKRVACYAGFACFLLTSYFVLHGRTAAEQKKSGRSVLEQLLAR
                     GVDPRVSKIIPETLVVAPQPALIRREIPVPTEPRTVRLRGQTATVYPNIQMHPSTTTT
                     QSEMSIATDPVNPNVVLAGSNAVSVFVDFGSQGWYHTTDGGVNWKGGDTLPTHTNPST
                     FMSDPAVGIDLGANLFFSALLLGAQSSDVVIAKSTNGGAVWTQTFVPDPSNDQDKDHF
                     TVDVNPSSPHAGNLYAAYTDFEEVVPPIRFSRSIDGGATFSSPVSISNGVGKLFNQGV
                     NMAVGPDGELYAVWTGYDFFPLQPGDSTRLGFNKSTDGGQTWQAAKTIRNVADIRGYL
                     NKGGNIIRVASFPSMAVDRSAGPRRGWIYVVYAQIATTTLTADVFLIRSTDGGTTWNS
                     PKKVSQENTATDQWFPATAVDPATGVLYVDYYDSRNFADDSAQVYVSISSDGGDTFQD
                     VLVSDAPFLPASIPGLAGGYMGDYIGIAAVNGTVWPSWNDNRTGIHQAYTARMVTSTV
                     SFPAKIRVSPDTLDFGARFIGFPETLAVLVRNLGTPDTLVVSGIGSDNPLFVPDADSF
                     SLTGGAFRLVNVVFSPVAPGSFTGHLSISSSDSTQPLVTVALRGSAALPPQLAVLPDS
                     LRVDLTTGESKAETLLISNPGGSDLSFQILKGAAGPALQANFNAAPSLPTASPLATGR
                     PVTLSELKEMQAGIPETLQNFPDKLPDAIPGYNPTVPLEFSGAAAADSVEIFGGLQFP
                     FTGGFRLRGNMFFCTDSANLTEHRFWLKTFGPTDLWFLVYEGTRPPLPQFFSAGRLIS
                     ASYVKTSGAAEGWYSSGNVDVPLTPGQHYLIVTQFRQACTYYYQLCTSPCVSYPISTS
                     FGRLEGPILLGNWNGGPIYAVPPETLQFVPWYALSLQGAYSAHQTLVTGPGTSWLAAS
                     ADTGTVPPGGSRALPVVFDSKAFGSPSVGAGDYFANLIVSANFPATPAPAETVKVHLH
                     LTAAPNLVFFGDSLDFGEAFVGFPDTLPFIVSNTGAETLRVSGITTSNPRFTLLNSPV
                     FNLLPGQSRTLQVQFLPSGLGVETALLSISSNDPNEPVKNGFLKGAAVHPPVLSPAPD
                     SFAVTLNQGKSLATTLSLHNTGLGTLSFSLHISPRPKVLLLSTDIDPFQIGDVQAKLL
                     ATGRFSVVDTIQLRFVDLPTMDQLRQYDAVLIWSGNLFMQPSAAAVGDLLADYVDMGG
                     GVVTAFFAMDNDPRFPPGNMIEGRFNAENYWALQPGPPKRGRATLGTIHRPGHPTIQG
                     VKLFDGGDFSDRLQGGTVALGGTLVASWSDGEPLVAFKGKKRVDLDFYPVSDSANCYQ
                     CNIGRWVSTTDGAILMADALEYVAGIAALAPNAGTAPPGGTANLSLSLAADVPRPGAY
                     CADLIISSNDPTRRVDTIPVSLTVPAGPNLLPAKSSLDFGDAYVGFPDTLALRVYNNG
                     SAPLSVSGINATNPRFSVLGSTAFSVLPGQFQDLKARFLPTSVGTDTGTLAIASNDAA
                     DPTVAVPLQGKGLLAPNISFSADSIFSTLLPGGKSALTLIIHNPGATDLKFSASISRT
                     PPVEALLLRNGVASLVPTTEKVAAPGQVIRTILPDGNPRGLTFLRGHLWQIKAIYPGG
                     GSGIGNDSIIELDPNTGQELSGFRLQEWDFYQGLTSDGTDLWVLRHPGFPTRADKYSP
                     DGRFLASLPVPGSSTGGGAFGLAFDGVALRSIVGDDTSGLFFAHFTPSGTLLENIAIP
                     FVPSSAFEQPNDMEWVAKHTGGHLWVGSGSRLFANQFDLSTNPAVLIQNFPFPTPFGT
                     EYGNGITHDGENLFFAAGDLINPPVIYVADDGLDEFVSAQPESGLIPAGGSANLTVSF
                     DASTLASGLKPGDYRTHLVFSSNDPSRPNVAVPVRLIVAQTCVTRGNVDGNSTIDIAD
                     IVALVQNVVFDTPLANPTAGDANCDGVVDLVDIVLLVQFVVFGTPQPCCL"
     gene            complement(10385..10909)
                     /locus_tag="AC16FU_04635"
     CDS             complement(10385..10909)
                     /locus_tag="AC16FU_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR2993694.1"
                     /translation="MERKVLRNKMAMNPCNCGFFGDPGHECSCTPTQIQKYMSKISGP
                     LLDRIDIHIEVPAVKFRELAGQPTGEPSAAIRERVNRARQVQLERFKSEKSIYCNAHM
                     QTKEIRKYCLIDDKSQELLRLAMQKLGLSARAYDRILKVARTIADLEGSVEIQSQHVS
                     EAVQYRSLDRNLWL"
     gene            10939..11103
                     /locus_tag="AC16FU_04640"
     CDS             10939..11103
                     /locus_tag="AC16FU_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2993695.1"
                     /translation="MSSIGDHIRKRRLDLGLWQEQVARLIGVTESTVTNWEMQRNTLR
                     YGSCQRFSGS"
     gene            11079..11309
                     /locus_tag="AC16FU_04645"
     CDS             11079..11309
                     /locus_tag="AC16FU_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2993696.1"
                     /translation="MPAIFRFLGYDPYPAEPSQPLSARLKACRRQLGLSQERLAGLLK
                     VDESTIAHWEQGRNRPSRRLLQAIDAFLASQP"
     gene            complement(11497..>11865)
                     /locus_tag="AC16FU_04650"
     CDS             complement(11497..>11865)
                     /locus_tag="AC16FU_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993697.1"
                     /translation="SLIAGGGAYSLASGQSRTVTVRYAPLPNSPGVLTCAVRTGNLAC
                     PVVNLTGAIAGCVLRGDVTNDAVADITDIVALIQNVVFDIGLPNPQAGDTNCDSVRDI
                     SDIVLLIQYVVFGNPTPCCL"
BASE COUNT         2513 a         3505 c         3245 g         2602 t
ORIGIN      
        1 attctttaca aacgaactac ttatagcgaa acctcgccaa attccccgac accggatttt
       61 cactgaagct tagtacactg tagaaatagg cacagacttt caactatagc cagaggtttc
      121 ggtccagcga tctgtattgc accgcttccg acacgtggtg cggctgaatt tcgacactcc
      181 cctcaaggtc ggcgatggtc cgcgacactt tcagaatgcg gtcatacgcc cgggcggaga
      241 gacccaactt ttgcatcgcc agccgcagga gttcctgcga tttgtcgtca atgaggcagt
      301 acttccggat ttctttcgac tgcatgtgtg cgttgcagta aatcgacttt tcgctcttga
      361 aacgctccag ttgcacctgc cgcgcccggt tcactcgctc acggatagcg gccgaactct
      421 cacccgtcgg ctgaccggct aattcacgga atttcaccgc gggcacttcg atgtgaatgt
      481 caatccggtc taagagcggc ccagaaattc tcgacatgta cttctgaatc tgcgtcggcg
      541 tgcaggaaca ctcgtgaccg gggtcgccaa agaagccaca gttacagggg ttcattgcca
      601 ttttgttacg caaaactttg cgctccaaaa ccactcccaa aagcataccc taagtcgttg
      661 tccaccatag gagaccacat ccggaagaga cgacttgacc tgggcctctg gcaacggcag
      721 gtggcccagc agatcggcgt ggatgagagc accataacta attgggagaa gcaccgaaac
      781 tttccctccc tacggttcat gccccggatt atccagttcc tgggctatga tccctgtcca
      841 gccgaggccg accagcccct acctgcccgg ctcaagacct gccggaaagg acttggactg
      901 tcccaaaaac gcttggccaa aatcctccgg gtggacccaa ccaccgttgc cagctgggag
      961 gccgcaacga accggccaac cgccaagtcg ataaaactca ttgatagctt tctcacttct
     1021 caaactctgg ccgccttagt ctcttccgaa tgacctaagt tctaaagtta cggccatcgt
     1081 tttaaaccgt accgaggcgt tggacatcag cgaaaagcct tcgggaggtt ggcgaaaacc
     1141 ccttgtctgc ccgctctcat tggactgagc aacgctaagg ctgagcaagt gaggccatag
     1201 gcgtcggtca acaaacgtgt ctttgctagt aagaagcttg gtgatcagga cgtctatatg
     1261 atcggtgagc tcgcaaagaa cactgaagat ccgaagaact cgtggatgcg accatcacta
     1321 gtttcggaga cactcccctg ttagtagtga agctacgttc acgagcgtag ggtcttgttt
     1381 gagcagtatc ccgtgggcct cgagcactcg gagaatgtag cggttctctc gtgcaaggcg
     1441 gtctaaagcg gcaggttcat ctcgagtgtt ggctcaccat tcgaacaaag tcgaccaagt
     1501 ttgggaaaaa gtcgccagcc gtgtgaatga taatcagtgc cgcgaggtta cgcaaccgtg
     1561 cccacagtac accaagaaag aagccggtga ccgacgtaac ggtaatggca tatccgacag
     1621 ccttgataag ggagggtgcg gtacctagcg cttcaccggt cgtctcaggg cgcaagtaga
     1681 gaccgggcgc atgggctagg ccaaagagga ctgcgacaag aacgatagcg gtgcactccg
     1741 atccggtcca gacggcaagg cgggtctgga gaaggccccg gaagaaaaac tcctctacca
     1801 gaccaacttc gaccaaaagc cagccaaacg cgaggaagga agctattcca aggacaacag
     1861 gggaatgggc ggtttctgct aaagctttga gggcacgacc gaagacgagt tgaaagacta
     1921 tcagcacgcc agccatccca agcaccgggc gccagtgacc tttgaggcga atcggcaaac
     1981 cgaggaggca agccggtgtg tacccccaga gaaacttcca gaggatcaat ggaaccacca
     2041 caaagacaag cagtttgcca gtgagaaggg caaggctgga ggaaaattgt gtgcttccca
     2101 gggtacgtag gaagggaatg ccccaggtga gaaaagccac gataacaaag agatagccga
     2161 ccagtagcct tgactccgcc cttggatggt tcactgggaa agagtgggct tttcgaccgc
     2221 gagtcagcca cactgccaaa agggagaaaa gtccgcccac gacgagaagg acaagaagcg
     2281 gttccgtagg ggagaagtgt tcgcggtaat aaaggagagc gaggaggctg aagtagccga
     2341 tgatgtagac ccacacggca acgtgacgaa ggaaatgtga gggcggtagc attttcgatg
     2401 tcctttctcc gtcagggttt acgcacgtcc agcactaata gccggccggt cgcgtagtca
     2461 acggtcagga taatctatgt tagaatgtca gagtcgatcg agagctgtac ccgcggtgat
     2521 agagtcaagt cgatgcccga ccggacatgc tctatgacga cggcaccaca ttgatgagcg
     2581 gaccaatagc aagtgagtca atgcgtaccc cattgactgc tcctgcaatc gtttgatgca
     2641 ccttcgtctt atctaaaacc ggcaaaagga cgcacaggtt tcacgtctgg agcggaatat
     2701 aatcaatgcc cggggatgcg gcagagaaat ttgcgtccag ggagcatcaa cgggaaaacc
     2761 tgtccccaaa tttgaagcga tcggttcggc cccccccccg acatcgctgg ttcgattccg
     2821 ccttaggcgg attcacctca actcgggaca agttcgctaa ccgcaagtac agtaccgcta
     2881 tgggaataaa gtgaatacat tgcctcccag gaacaatctg acataaggta tggtttaagg
     2941 tagtgatacg cgcattctgt ttaggatggg cagtgccgaa tcgggtgtcc ccgccgaagg
     3001 gaggctgtga gctacgaggc caagagcccg gaaaattatg attaaaataa tataaaaagg
     3061 taatatagtc cttgacaaga ctaaactggt agagtatttt ctcgtaagaa cacttgctgc
     3121 aactcggtct gagaaaagag acgcgtttgg gaagttaacg gcagcattca cccaacttta
     3181 tctggaggtc gtatgtccag cgctttacgc ttcgatatcc ggcgcggtgc aggattggcg
     3241 gctttgctca tcctgtcatt ttctttctca tccgcttccg gccagttcac cttgctgtac
     3301 cagaaaaacg gcgccgcggc cagcgacttc ttcggccgct cggtgtcttc ggccggcgac
     3361 gtgaacggag atggcaaagc cgattttatc atcggcgctt ataacaccgc tccccgcgac
     3421 ataaatggta ttggagctgg ttcggcttat gtctattccg gagttgacgg cgcactcctt
     3481 ttccagagaa acggcgccgc ggccggggac ttgctgggct actccgttgc ctcggccggc
     3541 gatgtggatg gggacgggaa agccgatttt atcatcgggg ctcctcaagc ccacgtgggc
     3601 agctttataa ataacatcgg ctcggcctac gtctattccg gcgccaccgg cgccctgctc
     3661 taccagaaag acggcgccac gaccggtgac ctttttggaa cctccgtggc ctcgtcggga
     3721 gatctgaaca gagacgggaa gtctgatttt attgttgggg ctaataatgc ccagccgggc
     3781 gggcttacct cggccggctc ggcgtttgtc tattccggcg ctaccggcgc ccttttgtac
     3841 cagaaaaacg gcgccgcggc atttgacaac tgtggctatt cggtcgcctc ggccggagac
     3901 gttaatgggg acgggaaacc ggatttcatt atcggggcta atgttgccga cccgggcggg
     3961 cttacaagtg ccggctctgc ctatctttat tcaggcacca ccggcgcttt gcttctccag
     4021 aaagacggcg ccacgaccgg tgaccaactg ggcatttccg tggcctcggc cggggatgtg
     4081 aacggagacg ggagggctga ttttatcatc ggggctcatg cggccagtcc aggcgggctt
     4141 tttaatgccg gctcggcgta tgtttacagt ctgccgtgcg tggtgcgcgg cgatgtgacg
     4201 aacgacgcgg cgaccgacat cgtggatatc gtggccctgg ttcagaacgt ggtgtttggc
     4261 acgcccctgc ccaatccgct cgccggtgac gtcaactgcg acacggtgcg ggacgtcacc
     4321 gacattgtat cgctcatcca gtacgtcgtt tttgggacac ccacgccctg ctgtttgtga
     4381 aaaaaggagt agttatgaca aaaagagttg cctgctacgc cggtttcgcc tgtttcctcc
     4441 tcacctcgta cttcgttctt cacggccgga cggcagccga gcaaaaaaaa tcaggccgtt
     4501 cggttttgga acagctttta gcccgcgggg tcgaccctcg agtctcaaaa ataattcccg
     4561 aaactcttgt tgtcgcgcca caacctgctc tcattcgacg tgaaatccca gtccccaccg
     4621 aaccgcggac ggtccgtttg cgggggcaaa ccgccaccgt ttaccccaac atccaaatgc
     4681 accccagcac aaccaccacc cagagcgaga tgtcgattgc gaccgacccg gtcaatccga
     4741 acgtcgttct ggctggctct aacgccgtca gcgtcttcgt cgattttggc agtcagggat
     4801 ggtaccacac caccgatggc ggcgtcaact ggaagggcgg cgatacgctt ccgacccaca
     4861 ccaacccctc gacgtttatg tccgacccgg cggtcggcat cgacctcggc gcgaaccttt
     4921 ttttcagcgc ccttcttttg ggcgcccaaa gttcggacgt ggtcattgcc aaatcgacca
     4981 acggcggagc ggtctggacc cagaccttcg tcccggatcc aagcaacgat caggacaaag
     5041 accatttcac cgtggacgtt aacccttcca gcccgcatgc cggcaacctt tacgccgctt
     5101 acaccgattt tgaagaggtt gtgccgccca tccgtttctc ccgttccata gatggcggag
     5161 ccaccttttc aagccccgtt tccataagca acggagtggg gaaactcttc aaccagggag
     5221 tcaatatggc ggtagggccg gacggagaac tgtatgccgt ttggaccggg tatgactttt
     5281 ttccgctcca acccggcgat tccacccggc tgggattcaa caaatcgacc gatggcgggc
     5341 agacctggca ggcggccaaa accatccgca atgtggccga catccgcggt tatttgaaca
     5401 aggggggaaa tatcatccgg gtggcgagct tcccgtccat ggcggtggac cgctcggccg
     5461 gccccagaag gggatggatt tatgtggtct atgcgcagat agcgacaact acgctcaccg
     5521 ccgacgtttt tctgattcgt tccaccgatg gcggtactac ttggaattcc cccaaaaagg
     5581 tgagccagga aaacaccgct actgaccaat ggttcccggc gaccgccgtt gaccccgcca
     5641 cgggggtcct ttatgtggat tactacgaca gccgcaattt tgccgacgac tccgcccaag
     5701 tttatgtcag catctccagc gacggcggcg acaccttcca agatgttttg gtcagcgacg
     5761 cgccttttct tcccgcttcc ataccgggcc tggcgggagg ttatatgggg gactacattg
     5821 gcattgccgc cgtaaacggg acggtttggc cctcctggaa cgacaaccgc accggaatcc
     5881 atcaggccta cactgcccgg atggtcactt cgaccgtcag tttcccggcc aaaatcaggg
     5941 tctcgccaga cacgctcgat tttggcgccc gtttcatcgg ctttcccgaa accctggcgg
     6001 tattggtgcg caatctggga acaccggata cgctggtggt atcgggcatt ggttcggaca
     6061 accccctgtt cgttccggat gcggacagct tttcccttac gggaggcgcg ttccgtttgg
     6121 tcaacgtggt gttcagcccg gtggcgcccg gttctttcac cggccatttg agcatctcca
     6181 gcagcgactc cacccagccg ctggtaacgg tcgccctgcg gggcagcgcg gcgctccctc
     6241 cgcaactcgc cgtacttccc gattccttgc gggtcgattt gacaaccggc gaaagcaagg
     6301 ccgaaacgct tttgatttca aatccgggag gcagcgacct ctcctttcag attttaaagg
     6361 gcgccgccgg gccggccctc caggcaaact tcaatgcggc tccatccctc ccgaccgcct
     6421 ctcccctcgc caccggccgc ccggtcaccc tgagcgagtt aaaggagatg caggccggca
     6481 taccggaaac gctccagaat tttccggata aacttccgga cgccattccg ggttataacc
     6541 caaccgttcc gctggaattc tccggagctg cggcggccga ttcggtggaa atttttggag
     6601 ggctgcaatt tccgttcact ggcggattcc ggttgcgggg caacatgttt ttctgcaccg
     6661 actccgccaa tctgacggaa caccggtttt ggctgaagac cttcggcccg accgacctgt
     6721 ggtttttggt gtatgaaggg acccgccccc ccctgccgca gtttttcagc gcgggacgcc
     6781 tgatcagcgc atcctatgtc aaaacctccg gcgcggcgga gggatggtac agttccggca
     6841 acgtcgacgt tccgcttacc cccggacagc attatctgat tgttacccaa tttcggcagg
     6901 cctgcactta ctactatcag ttgtgcacct ccccctgcgt ttcgtacccc atctccacct
     6961 cattcggccg gctggaaggc ccgattcttc tgggcaactg gaatggcggg ccgatttatg
     7021 ccgtcccgcc cgagacccta cagttcgtcc cctggtacgc gctctccctg caaggcgcct
     7081 attctgccca tcagacactg gtgaccgggc cgggcactag ctggctggcg gcttcggccg
     7141 ataccggcac ggttccgccc ggcggcagcc gggccttgcc ggtcgttttt gattccaagg
     7201 cctttggctc ccccagcgtg ggagcggggg actactttgc caatctaatc gtctccgcca
     7261 actttccggc cactccagcc ccggccgaaa cggtaaaggt acatctacat ttgaccgccg
     7321 cccctaacct cgttttcttt ggcgactctc tcgattttgg agaggcgttc gtgggttttc
     7381 cggacacgct tccgtttatc gtctccaaca ccggcgccga aacgctgcgg gtgagcggaa
     7441 taaccacctc caacccccgc ttcactctct tgaactctcc ggttttcaac ctgcttccgg
     7501 gccagtcgcg caccttgcag gtacaatttc tcccaagcgg cttgggagtc gaaaccgctc
     7561 ttctctccat ctcttccaac gacccaaatg aaccggtgaa aaacggattt ctcaaagggg
     7621 cggccgttca tcccccggtc ctttctcccg cgccggactc ctttgcggtg accttgaatc
     7681 agggaaagag cctggccacg accttatctc ttcacaatac cggattggga acgctttcct
     7741 tttctctcca catttccccc cgtccaaaag tcctgcttct ttccaccgac attgacccat
     7801 tccagattgg ggatgtccaa gccaagctcc ttgccaccgg acggttctcc gttgtggata
     7861 cgatccaact tcgtttcgtc gacttgccga ctatggacca actccggcag tatgacgccg
     7921 tcttaatctg gagcggcaat ctatttatgc agcccagcgc ggcggctgtc ggggacttgc
     7981 tggcggatta cgtcgatatg ggcgggggcg tggtaaccgc cttctttgcg atggacaacg
     8041 accccagatt tccccccggc aatatgattg aaggccgctt taacgccgaa aattactggg
     8101 cgctccagcc ggggccgccc aagcggggca gggccacctt ggggaccata catcgtcccg
     8161 gccacccaac catccagggt gtaaaacttt ttgacggcgg ggactttagc gaccggctgc
     8221 aggggggcac ggtcgctttg ggcggcacgc tggtcgcttc ctggagcgac ggcgaaccgc
     8281 tggtggcttt caaggggaaa aaacgggtgg acttggattt ttatccggtc tcggactccg
     8341 caaactgtta ccaatgcaac atcgggcggt gggtctccac caccgacggg gccattttaa
     8401 tggccgatgc cctggaatac gtggccggca tcgccgctct ggcacccaac gccggaacgg
     8461 cgcctcccgg aggaacggca aacctttccc tatcgcttgc cgccgacgtg ccaagacccg
     8521 gtgcgtattg cgccgacctg attatttcga gcaacgaccc gacccgccgg gtggacacca
     8581 tcccggtttc cctgacggta cccgcaggtc ccaacctgct tcctgccaaa tcttcgctgg
     8641 actttggcga cgcctatgtc ggatttccgg acacgcttgc gctccgcgtc tataacaacg
     8701 gttcggcgcc gctttcagtc agtggtataa acgcaaccaa cccccgcttt tcagtcctcg
     8761 gttcgacggc cttcagcgtt ttacctggtc agttccagga tttgaaagcg cgcttcctcc
     8821 caacatcagt cgggacagac accggaacgc tggcgattgc gtccaacgac gcggccgacc
     8881 caaccgtggc cgtgcctctg cagggcaaag ggctcctggc gccgaacatt tctttttcag
     8941 ccgattctat attcagcact cttctccccg gtggaaaaag cgccttgacc ttgatcatac
     9001 ataaccccgg cgccaccgac ctgaaattct ccgcttcgat cagccgaacc cctccggtgg
     9061 aggcgctact cttgcgcaac ggggttgcat cgttagtgcc taccactgaa aaagttgccg
     9121 cccccggtca ggtaattcgt accattctgc cggacgggaa cccaagggga ttgacctttc
     9181 ttagaggaca tctgtggcaa attaaggcaa tctaccccgg gggcggctct ggcatcggca
     9241 atgacagtat cattgagctg gacccaaata caggccagga attatccggt ttccgcctcc
     9301 aggagtggga tttctaccag ggtcttacca gtgacggcac cgacctgtgg gtgctgcgtc
     9361 atcccggctt tccgacccgg gcagacaagt acagtccgga cggccgcttt cttgcctccc
     9421 tgccggttcc cggatccagc accggcggcg gcgctttcgg cttggctttt gacggtgtcg
     9481 cactccggag catagtcggc gacgacactt ccggactttt ctttgcgcac tttaccccct
     9541 ccggcaccct gctggaaaac attgccattc ccttcgttcc ctccagcgcg tttgaacagc
     9601 caaacgatat ggagtgggtg gccaaacata cgggcggcca tctatgggta ggtagcggta
     9661 gtcggttatt tgcaaatcaa ttcgacctct ccactaatcc agccgtttta atccagaatt
     9721 tcccctttcc gacgccattc ggcacggagt atggcaatgg aattacccac gatggggaaa
     9781 acctgttttt tgccgccggt gatttaatta atccgccggt catttacgtg gcggacgacg
     9841 ggctggacga gtttgtttcc gcccaacccg aaagcggcct cattccggcc gggggaagcg
     9901 cgaacctgac tgttagcttt gacgcttcaa ccctggcctc cggcttgaaa cccggggatt
     9961 atcgcaccca cctggtgttt tcaagcaacg acccatcacg tccgaacgtg gctgtaccag
    10021 tgcggctaat cgttgcacag acttgcgtaa cgcgcgggaa cgtagatggt aattccacca
    10081 tcgacatagc agacattgtt gctttggttc agaacgtggt gttcgacaca cccctggcga
    10141 atcccacggc gggtgacgcc aattgtgacg gtgtggtgga cctcgtggac atcgtgcttt
    10201 tggtgcagtt cgtggtgttc gggacgccgc aaccgtgctg cctctgaaaa aggggtagct
    10261 gtgggagtta gaaaaatttg ggcaatgaca gcgtgctaat ctatcgactc tactcaaatt
    10321 tgagagggcc tgcttcctct gttttgggtt cgtatgcttg tgggaggcgt cggcgttctc
    10381 ctacttacaa ccacaggttt cgatccagtg accggtactg caccgcttct gaaacgtgct
    10441 gcgactgaat ttcgacgctg ccctctaagt cggcgatggt ccgcgctact tttaggatgc
    10501 gatcatatgc ccgcgcagag agtcccagct tctgcatcgc cagccgcaag agttcctgag
    10561 atttgtcgtc gatcagacag tatttccgga tttctttcgt ctgcatgtgc gcgttgcagt
    10621 aaatcgactt ttcgcttttg aagcgctcca gttgtacctg ccgcgctcgg ttcacccgct
    10681 cgcggatggc agccgacggc tcgccagtcg gctggcctgc taattcacgg aatttcaccg
    10741 ccggtacttc gatgtggatg tcaattcggt ccaagagcgg cccggaaatt ttcgacatgt
    10801 acttctgaat ttgcgtcggc gtgcaggaac actcgtgacc gggatcgcca aagaaaccac
    10861 agttgcaggg gttcattgcc attttgttac gcaaaacttt gcgctccaaa accacttcct
    10921 aaagcctacc ctaagtcatt gtcttccata ggagaccaca tccgaaaaag acggcttgac
    10981 ctcggtcttt ggcaggagca ggtcgcccgg ctgatcgggg tgactgagag taccgtaacc
    11041 aactgggaga tgcaacgcaa cacccttcgc tacggttcat gccagcgatt ttccggttcc
    11101 taggctatga tccctatcca gccgaaccca gtcagccctt gtcagcccgg cttaaggcct
    11161 gtcgcagaca gttggggctg agccaggagc ggctggccgg attgctcaaa gtggacgaga
    11221 gcacgatcgc acactgggaa caggggcgga accggcctag cagaaggttg ttgcaagcta
    11281 ttgatgcctt tctggcttcc cagccttaac gcccagcggc tgacgtatca tcgggctatg
    11341 tcaggtccgg gcttaattga cttaagacaa agtaagccgg aaaacatgcg actccccttg
    11401 cagttatcgt ttccttcttt tttgattttt caagcgcggg ttggtctcta cagccggttc
    11461 aagttacccg ccaaattctg aatcgaatcg gggcgcttac aagcaacaag gcgtcgggtt
    11521 accgaatacc acatactgga tgagcagcac aatatctgag atgtcccgta cgctgtcgca
    11581 gttggtgtct ccggcctggg gattggggag tccaatatcg aacaccacat tctggatgag
    11641 ggccacgatg tcggtgatat cggctacggc atcgttggtc acatcccccc gaagcacgca
    11701 gcccgctata gcaccggtta agttaacaac gggacaagcg agattgccgg ttctcaccgc
    11761 gcaggtcaac accccagggg agttggggag gggagcatag cgcaccgtga cggtgcggga
    11821 ttgtcctgac gccagggagt aggcacctcc tcccgcaatg aggct
//
LOCUS       DBNGZA010000224         6241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2056130, whole genome shotgun sequence.
ACCESSION   DBNGZA010000224 DBNGZA010000000
VERSION     DBNGZA010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2056130"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="pdxA"
                     /locus_tag="AC16FU_04655"
     CDS             <1..876
                     /gene="pdxA"
                     /locus_tag="AC16FU_04655"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR2993698.1"
                     /translation="FAPKVTLVPVEGESYPHLKANTVGVQDVSTKENVAIEPGKLSAE
                     AGRLAGRAIEEAVRFVLRHRWPARVTAPVTTQTLSLAGYRFTGHTEYLAQATGVADSA
                     MFFVTGDVKVVCVTTHIPLAEVTRVLTPELIERKILLAADALRNYFGLKRPRVALCGI
                     NPHAGDGGLLGSDEAGLIEPVIRRLSQRGMAISGPYSADSVFLPQVRRKFDLIAAMYH
                     DQGMIPVKLLGRGRAVNITLGLPFVRTSPAFGSAFDIAGKGQADAAGMAAAIRWAVRL
                     GEVDLRRRGKGNAQG"
     gene            917..1651
                     /locus_tag="AC16FU_04660"
     CDS             917..1651
                     /locus_tag="AC16FU_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR2993699.1"
                     /translation="MDHNLLLEVADLSHHYQKPVFSDISFSVGAGEIVGIVGPTGAGK
                     TALFNCLIGLAKPTSGSITYADTILPATQKPGPRLRRHLGLVFQDDHLFFDRTVFDNI
                     ALPLLLLKKSSPEIRRRTTAVATRIGLSAVLLSRCGSLSAGERRLVALARAVVHEPPL
                     VLADEPLGPISPPAGEVLGLFRELGDKGTGFLLFSSVVDLIEPYCTRLHVLKEGSLVP
                     VAAATPPESPRPISIPLEPSSPAVSA"
     gene            1648..2511
                     /locus_tag="AC16FU_04665"
     CDS             1648..2511
                     /locus_tag="AC16FU_04665"
                     /inference="COORDINATES: protein motif:HMM:NF037183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR2993700.1"
                     /translation="MTPFYHTKESFRWLSHSLGATLLTIVAFAVVVVLFFLGWQANGK
                     VEAKLLQLEERFVLSVVLADDLPPGTEEELTRQLERLPAVAAVAFQSKAEALSALEKE
                     LETEMGTELAANPLPASFRLQLKREAFTTDRLAQLVTTVRSFSGVREVIYPETWAEQY
                     GRLAAVLGSGRLAFLGIFVALAGLLVAAAVRQLFCDRKDDVFTLRLLGAPWSFALAPY
                     LVAGGVIGLGGGLFGLLAAWGLTTGFSRMAGIRFGPLLPGGYFFAGAAVLLSMLLIGT
                     APLMFRRHSPP"
     gene            2508..3716
                     /locus_tag="AC16FU_04670"
     CDS             2508..3716
                     /locus_tag="AC16FU_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR2993701.1"
                     /translation="MTRLRYTLAALGLFLLAGVVGPRVLAQEENDTARVSGEAERAEL
                     QNIRNQIASQKTTLTALEKKEGTLAAQLTHVEEKLRLSDQLIGRLKSRLARLNRQIKT
                     TDVGLSLTEGQLAAQQVALAELLSAFYYQSREYSPPAGLLFIPPEQALREAEVRAGTA
                     RLARALHQQVQLVASQKGELSLNRSLLEARQKELLSLKRETERTERQRRQDLEKKGQL
                     LAEIRAQKQETASQVAELEKSAEALEKILAKIEKRRRARPKPEARPAWQLPAGEFARH
                     KGSLPWPVAGAVVSTFGPKKDPVKKTVSHQPGIDIAAGAGAQVRAIAAGVVAYVGFLR
                     GYDRFVILEHDAGYYTLYAHLEGVQVTEGQVVQSGQTIGSVAGEKNDALLHFEIRQGK
                     QQLNPQEWLQ"
     gene            3716..4816
                     /gene="holB"
                     /locus_tag="AC16FU_04675"
     CDS             3716..4816
                     /gene="holB"
                     /locus_tag="AC16FU_04675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR2993702.1"
                     /translation="MDRGKVSGQTRLIELLQRAIAHDRLAHAYLFWGPEGIGKWALAI
                     ELAKVVNCEKRRAEPCGICPACRKIDALSHPDLELLYPLPKSRKSRSDDEEDDADEES
                     RLFFLSEKRKNPYRIVRFERYKTITMARIRETQSRLALKPFEARKRVLIVADAETLRS
                     DCANILLKTIEEPPPETLVILTTQNPSLLLPTIVSRCQLLRLSPVPDSTITSFLKEQT
                     DADQESVSRAVALAGGSPGKALEFLDPEIQNLLNQAKAVWSEVSHSEMAALVGLRALV
                     YQLPRDRIFLLLDFWANQICAAWAESFDTPQAARQTALPLVSAQKILSDLEQIRAHLW
                     VNVNASLALGAFLLRAGKLLRSAPATASAASS"
     gene            complement(4791..5108)
                     /locus_tag="AC16FU_04680"
     CDS             complement(4791..5108)
                     /locus_tag="AC16FU_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993703.1"
                     /translation="MFDKPTANTGSPRNFELHQNVPNPFNPTTAITFYLPTETQVELD
                     IFNLLGQRIVRLVDGKLNAGEHMVSWAGQNEHGQSVASGPYFYRLRTDTGFLQIRKML
                     RLR"
     gene            complement(5130..>6241)
                     /locus_tag="AC16FU_04685"
     CDS             complement(5130..>6241)
                     /locus_tag="AC16FU_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993704.1"
                     /translation="WLMKFGADDVNFATLPGRDSLLGYGRINIRKTFKLLDDIQAGTL
                     AWIPLRIPGGTAIADGQVILRLPDRPLTIQNATRYKVEREVTSAAVFPNFPLVYPRKS
                     TVGWDSSRTNFEVGWLEVISVNARGWKMRTYVFQDNSTLQWYPTSPANVQFDVVVITP
                     PYFTPDFGLTTKVPGNSNQIQLNWENAGATNYYVERQESPTSPWVTLDTLPGSQFSYI
                     DGPSLVNPQKGSHTYNYRIRSNTGLTSNESSSKTFPNPPNITSATVHLGPECHTGGEG
                     MMGPVPPPGPNLVPTNQIDISWATDPNQDVPIALYQVRFDHAGTPTYLPATTRLDTTL
                     YPLNFDADYTIYGYAYDQEGNLSRVAGAYTVHTGL"
BASE COUNT         1250 a         1763 c         1898 g         1330 t
ORIGIN      
        1 tttgcgccga aagtcacact cgtgccggtg gagggcgagt catatccgca tctcaaagcc
       61 aataccgtcg gcgtgcagga tgtatcaacg aaagagaacg tggcaatcga gccgggaaaa
      121 ctgagcgccg aagcggggcg gctggccgga cgggcaattg aagaagcggt gcggttcgtt
      181 ttgcggcacc ggtggccagc gcgggtaacg gcgccggtga ccacgcagac cttatccctg
      241 gccgggtacc gcttcaccgg ccataccgaa tatctggcgc aggccaccgg cgttgccgat
      301 tccgcgatgt tctttgtgac cggcgacgtg aaagtggttt gcgtcaccac ccacattccc
      361 ctggccgaag tcactcgcgt tctcacgccg gagttgatcg agcggaaaat tcttctggcg
      421 gccgatgctt tgagaaacta ttttgggcta aaacggcccc gcgtggcgct ctgcggaatc
      481 aacccgcacg caggcgacgg cggtctatta ggaagtgatg aagcaggact cattgagccg
      541 gttatccgac gactgtcaca gcgggggatg gccatatccg gcccctactc ggctgacagc
      601 gttttccttc cgcaggtgcg gcgcaaattc gatctcattg cggcgatgta tcacgaccag
      661 gggatgattc cggtgaaact tttgggccgg ggccgggcgg tcaacatcac gctgggattg
      721 ccgtttgtcc gcacctcccc cgctttcggc agcgcgtttg atatagcggg taaaggccaa
      781 gccgacgcgg caggaatggc ggccgccatc cgctgggcag tccggctggg agaggttgac
      841 ctgcgtcgga gaggaaaagg gaatgcgcaa gggtgagaag aacccgcgct ttgaccgata
      901 gagatttgta ggaactatgg accataatct tttgcttgaa gttgccgatc tttcccacca
      961 ctaccagaag ccggttttca gcgacatttc gttttcggtc ggagccggag agatcgtggg
     1021 catcgtgggt ccgaccggcg ccggaaagac ggcacttttc aattgcttga ttggcctggc
     1081 aaaacccact tccggatcaa ttacctatgc cgacaccatc ctcccggcga cacagaaacc
     1141 gggtccccgg ctgcggcggc acctgggctt agtttttcag gacgaccacc tctttttcga
     1201 ccggaccgtt tttgacaaca ttgcgcttcc ccttctgctt ttgaaaaaaa gttcgccaga
     1261 aatccgccgc cgcaccacgg ccgttgccac gcggatcggg ctttccgcgg ttttgctctc
     1321 ccggtgcggt tcgctttccg ccggggaaag gcgcctggtg gctctggccc gggcggtggt
     1381 ccacgagccg cctttggttt tggccgatga accgctgggg ccgatctcgc cgccggcggg
     1441 agaagtttta gggttgttcc gggaacttgg ggacaaaggc accgggtttc ttctgttttc
     1501 gtcggtggtt gatttgattg agccgtactg cacccggctc cacgtgctca aagagggaag
     1561 cctcgttccg gttgcagccg ccaccccccc ggaatcgccc cggccgatat cgattcccct
     1621 ggaaccatcc tcgcctgcgg tctcggcatg acgccctttt accatacgaa agaaagcttc
     1681 cgctggctgt ctcacagtct cggggccacc ctattgacca tagttgcctt tgcggttgtg
     1741 gtagtgctct tttttctcgg ctggcaggcc aatggaaaag tggaggcgaa gcttttgcaa
     1801 ctggaagagc gcttcgtcct ctccgtcgtc ttggccgacg acctgccgcc gggcaccgag
     1861 gaggagttga cccgtcagtt agagcgtctg ccagcggtcg ctgcggttgc ctttcaatcc
     1921 aaagcggagg cgctgtcggc gttggaaaaa gaactggaaa ccgagatggg gacggaattg
     1981 gcggccaatc cgttgccggc gtcattccgg ctgcagttga agcgagaggc ttttacgacg
     2041 gacagattgg cccaactggt aacgacggtg cgctcctttt ccggtgtgcg ggaagtcatc
     2101 tacccggaaa cctgggccga gcagtacggc cggttagcag cggtgctggg ctcgggccgg
     2161 ttggcgtttt tgggtatttt cgttgctctg gcaggattat tggtcgcggc cgccgtgcgc
     2221 cagctcttct gtgaccgcaa ggacgacgtc ttcaccctga gacttctggg cgcgccgtgg
     2281 tcgtttgctc tggccccata tctcgtggcc ggcggggtga tcggcctggg cggcggcctt
     2341 tttggtcttc tggccgcatg gggcctgacc accggatttt cccggatggc gggaattcgg
     2401 ttcggtccgc ttttgcccgg gggctacttc ttcgccggtg cggcggtgct tttgagtatg
     2461 ctcttgatcg gcaccgctcc actcatgttc cgccggcatt ctcctccatg acgcggctcc
     2521 gctacaccct cgcagcactg ggactttttc tcctggccgg ggtagtcggg ccgagggtat
     2581 tggctcagga agaaaatgac acggcacgcg tttccggcga ggcggaacgg gccgaactgc
     2641 agaacatccg caatcaaatc gcctcccaga agaccaccct gaccgctctg gagaaaaaag
     2701 aagggacact ggcggcacag ttgacccacg tagaggagaa gctgcgcctg tcggaccaat
     2761 tgatcgggcg cctcaagtcc cgcctggcgc ggttgaaccg gcagattaag accaccgacg
     2821 tcggcctttc gctcacggaa ggacaactgg cggcgcagca ggtggcgctg gcagaattac
     2881 tttccgcttt ctactaccag tcgcgagaat actccccccc cgcgggactg ctgttcatac
     2941 ctcccgagca agcgctgcgg gaagcggaag ttcgggcggg cacggcgcgt ctggcgcggg
     3001 ccctccacca gcaggtgcag ctggtagcca gccaaaaagg agagctctcc ttgaatcgat
     3061 cgcttctgga agcccggcaa aaagaattgc tttccctcaa acgggaaacg gagcggaccg
     3121 agcggcagcg gcggcaagac ttggagaaaa aaggacagct tctggccgaa attcgcgcgc
     3181 agaaacagga aaccgcctcg caggttgcgg aactggaaaa aagcgcggag gctttggaga
     3241 agattctggc gaaaattgaa aagagaagga gagcacggcc caaacccgag gcacgcccgg
     3301 cgtggcagct gccggctggc gaatttgccc ggcacaaggg gagtttgccc tggcccgtgg
     3361 ccggggcggt cgtctccact tttgggccca agaaagatcc ggtgaaaaag acggtgtcgc
     3421 accagcccgg cattgacatc gccgccggtg cgggggcaca ggtccgggcc attgcggctg
     3481 gagtcgtggc ctacgtcggt tttctgcgcg gctacgaccg cttcgtgatt ttggagcacg
     3541 acgccggtta ctacacgctc tatgcgcacc tcgagggggt gcaggtgacg gaagggcagg
     3601 tggtgcagag cggccagacc atcgggtcgg tggccggtga aaaaaacgac gcacttttgc
     3661 attttgaaat ccgtcaggga aaacagcagc tgaatcccca ggagtggctg cagtaatgga
     3721 ccggggaaaa gtttcagggc agacgcgcct gattgaactc ctccagcggg cgattgcgca
     3781 tgaccgtctg gcgcacgcct acctcttctg ggggccggaa ggcattggca agtgggcttt
     3841 ggccatcgaa ctggccaaag tggtcaactg cgaaaaaaga cgggcggagc cgtgcggcat
     3901 ctgtccggct tgccgtaaaa ttgatgcact gagccatcct gatctggaac tcttgtatcc
     3961 cttgccgaaa agccgcaaat cgcgcagcga cgatgaagag gatgacgccg acgaggagag
     4021 ccgtcttttc ttcctttccg aaaaacgcaa gaatccctac cggatcgtcc gattcgaacg
     4081 ctataagacg atcaccatgg cgcggattcg agagacccag tcccggctgg cgctcaagcc
     4141 gttcgaagcg cggaagcggg ttttgatcgt agccgatgcg gaaaccctgc gctccgactg
     4201 cgccaacatc ctgctcaaga cgattgagga gccgccgccg gaaacgctgg tcattctcac
     4261 cacgcaaaat cccagtttgc tcttgccgac gattgtttct cgctgccagt tgctccggct
     4321 tagcccggta cctgacagca ccatcacctc cttccttaaa gagcagacgg acgcggatca
     4381 ggaatcggtt tcccgcgcgg tggcgctggc cggggggagt ccggggaagg ccttggaatt
     4441 cctcgatccg gaaattcaga atcttctcaa tcaagcgaag gccgtctggt cggaagtgtc
     4501 ccattcggaa atggcagccc tggtcgggct ccgcgcgctg gtttatcaat tgcccagaga
     4561 ccgcatcttc ctcttgcttg acttctgggc gaaccagatt tgtgccgcgt gggcggagtc
     4621 tttcgatact ccgcaagccg cgaggcaaac ggcattacct ctcgtttcgg cgcagaaaat
     4681 cctctccgac ctggagcaaa tccgcgccca cctctgggta aacgtcaacg cttccctggc
     4741 gctgggggcg tttctgttac gcgccgggaa attgctccgg agcgcgcccg ctaccgcaag
     4801 cgcagcatct tcctgatctg caagaatccc gtatctgtcc gcaaccggta aaagtacggg
     4861 ccgctggcga cagactggcc gtgttcgttt tgacctgccc aggagaccat gtgttctccc
     4921 gcattcaact tgccatccac cagccgcact atccgctgtc cgaggaggtt gaaaatatcc
     4981 agttccactt gggtttcggt cggcaagtag aacgtgatcg ccgttgtggg gttaaatgga
     5041 ttgggaacgt tctgatgaag ctcgaagttc ctagggctcc ccgtgtttgc cgtgggtttg
     5101 tcgaaaatcg acccgcaccc cgcggcatcc tacaaaccag tgtgaaccgt atacgccccc
     5161 gccactcggc ttaaattccc ttcctggtca taggcatagc cgtaaatcgt gtagtcggca
     5221 tcgaaattca gcgggtacag ggtggtgtca agccgtgttg tggctggcag gtaggtagga
     5281 gttcctgcgt gatcaaaccg gacttgatag agagcgatag gaacgtcctg gttcggatcg
     5341 gtcgcccacg aaatgtcaat ctgattcgtc ggaacgaggt tcggtccggg tggaggcacc
     5401 ggtcccatca taccttcgcc accggtatgg cattccggac ccaagtgtac tgtggcagag
     5461 gtaatatttg gtgggttggg aaaggttttg gaggaacttt cattactggt aagcccggta
     5521 ttggaacgga tccgatagtt gtacgtgtgg cttcctttct gaggatttac caaggacgga
     5581 ccatcaatgt aggaaaattg cgaacccggc aacgtatcta gtgttaccca gggtgaagta
     5641 ggactctcct gccgttcgac gtaatagttg gttgcgcctg cattttccca gttcaactgt
     5701 atctggttag agttacccgg aactttcgtg gtcagcccga aatcgggggt gaaatacggt
     5761 ggcgtaatta cgactacatc gaactgcaca ttggcgggcg aagtcggata ccattggagg
     5821 gtactattgt cttgaaaaac gtaggtgcgc attttccagc cccgggcatt tactgagata
     5881 acttccaacc aaccaacttc aaaattcgtc ctactcgagt cccaacccac ggttgatttg
     5941 cgcggataga ctagaggaaa gttggggaaa actgcagcac ttgtcacttc cctctccact
     6001 ttgtagcggg tggcgttctg aatggttaag ggacgatccg ggaggcgcag gatcacttgg
     6061 ccatctgcta tggcagttcc gccggggata cgtaatggaa tccaggccaa tgttcctgct
     6121 tgaatgtcgt ccagaagttt gaaggtcttc cggatgttga tccgcccgta gcccagcaag
     6181 ctgtcccgac ccggcaatgt ggcaaagttg acatcatctg cgccgaattt catcaaccat
     6241 t
//
LOCUS       DBNGZA010000225         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2056137, whole genome shotgun sequence.
ACCESSION   DBNGZA010000225 DBNGZA010000000
VERSION     DBNGZA010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2056137"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="AC16FU_04690"
     CDS             <1..657
                     /locus_tag="AC16FU_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2993705.1"
                     /translation="FIIGAPGADVVPFDAGAAVAYNSLTGAALTASIFSVPPVPGDHV
                     GWSVGNAGFVTPIPDFIVGAPFALGTMGQTSVHTGPHPPSPTLPPILPGAAPGDNLGW
                     SVAGLMGSVDGDATDDIIVGAPGADMPPPDGGAADVYGFCAASGGLGDLDGNGVADIV
                     DIITLINNVVSGGPLPTGRACAELTGDAAMIGGDGIVDISDIICLINNVVFLSPIPCP
                     "
     gene            complement(976..2715)
                     /locus_tag="AC16FU_04695"
     CDS             complement(976..2715)
                     /locus_tag="AC16FU_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HIR2993706.1"
                     /translation="MTPQPKGYFSLVLHAHLPYVLAHGRWPHGTDWLSEAACETYLPL
                     LNVFNDLVATGIPPKVTLNLSPVLCEQLASHAFVAELLTYLDQKIKAAKQDRPAFKQK
                     GLIQLAATAAMWHDFYLGIKKDFEEKYNHDLIGAFRKLNDTGSIEVITCAATHGYLPL
                     LAQDMSVQAQVKQAVVAHQRHFGKKPRGLWLPECAYRPGYAWAPPVESPLGCTAHPRK
                     GVEEFLSENGIEFFFIDAALLKGGKAIGVYIDRFEALAQLWQQFEKEYRPAEENILRS
                     PREVYLVGLPEKKPVAVFTRDAETALVVWSGEHGYPADGNYLDFHKKHFPGGLRYWKV
                     TSAKSDLADKKEYEPEAVEARLEENANHFVSLVKREILAYYEQTKKPGLVVAPYDAEL
                     FGHWWFEGPRFLKKVLTKIAQDPEISLSTAGEYLDLAKPSTVISIPEGSWGEGGYHYI
                     WLNQWTEWTWQKIYSAEAELSSLLGRAGSSLSQPPLAHVLKQAARELLLLQASDWQFL
                     ISTWAARDYAELRLAEHFEKFSRLADFAEKMLNGLALTAEEEAYRRDCETQDNLFPDL
                     DLAWFQRVEFPAR"
     gene            complement(2900..3241)
                     /locus_tag="AC16FU_04700"
     CDS             complement(2900..3241)
                     /locus_tag="AC16FU_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR2993707.1"
                     /translation="MEIKPQPQQINVELGEKESEGIYSNLAFITHSPSEVIIDFARLL
                     PGVPRSKVYARVIMTPQHAKLLLQTLDENLKKYEAQFGQIKIFGKEDKGIGFKTVEPS
                     ATPVVRTDQPV"
     gene            3484..>4364
                     /gene="pfkA"
                     /locus_tag="AC16FU_04705"
     CDS             3484..>4364
                     /gene="pfkA"
                     /locus_tag="AC16FU_04705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR2993708.1"
                     /translation="MSTIKRIGVLTSGGDAPGMNSAIRAVVHEAVEKGLSVFGFSRGF
                     NGLIDNEGIELDLKAVGGLLQRGGTMLLSARSDEFKTPEGRKTARQSLEKRKVDALVV
                     IGGDGSFKGAQLLEEEGVAVAGVPATIDNDIYGTDMAIGVDTALNTIVEVVDKIKDTA
                     SSLERTFVIETMGRDSGYLALMSAIATGAEAAIIPEVPYDLEKIARRLYDRYQRGATN
                     SIIIVAEGASSAYEISRKLAKIASIETKITVLGHLQRGGSPSAFDRLLGSRLGPAAVE
                     ALLRGESGVMVGLVKRGV"
BASE COUNT          849 a         1223 c         1354 g          938 t
ORIGIN      
        1 ttcatcatcg gcgctccggg ggccgacgtg gtgccgttcg atgccggcgc ggcggtggcc
       61 tacaacagtc tcaccggtgc ggcgcttacc gcctcgatat tttccgttcc ccctgtcccg
      121 ggtgatcatg tgggctggtc ggtggggaat gccgggtttg tgactcccat tccggacttc
      181 atcgtcggcg caccctttgc cttgggcaca atgggtcaga cttcggtgca taccggtccc
      241 catcccccca gtccaacgct cccgcccatc ctcccgggcg ccgcgccggg tgacaatctg
      301 ggttggtcgg tggccggatt gatggggagc gtggatggcg atgcgaccga cgacatcatt
      361 gtcggcgctc cgggagccga catgccccct ccggatggag gcgcagccga tgtttatggg
      421 ttttgcgctg cctcgggcgg gttgggagat ttagatggca acggcgtagc ggacattgtg
      481 gatatcatta cgcttatcaa caacgttgtc tccggcggcc cccttcccac cgggcgggcc
      541 tgcgcggagc tgaccggcga tgcggccatg atcgggggag acggcatcgt ggatatcagc
      601 gacatcatct gcctgatcaa caacgtcgtg tttctctccc cgattccctg cccgtaaggc
      661 caggattcag aagcaaaagt agagcgtccg cgcggacgct ctactttttt tgggggaaaa
      721 agatgatcta tctgccttag tgctggccgc tgaccttctc ctcctatttc acacttcgtc
      781 cgttttaccc ctatgacaac cccaaattag cgcgggaagg ggcacctgtg tgcccccgtg
      841 gcgaccccaa agtaaacagg gattaggtcg gcacgacccg tatgcggatc agagatagga
      901 aaataaacat aaaagcactt gagtgacgca gacaagcccg gactatcttg gcttaaggtg
      961 tttcttaaag tgaacctatc gagccggaaa ttccacccgc tggaaccagg cgaggtcgag
     1021 gtcggggaag agattgtcct gcgtctcgca gtcacgacgg tacgcttctt cttcggcggt
     1081 gagggcgagg ccgttcaaca tcttttccgc aaagtcagcg aggcgggaga atttttcgaa
     1141 gtgttcagcg agccgcagtt ccgcgtagtc gcgggcggcc caggtggaga ttaaaaactg
     1201 ccagtcggaa gcctgcaaaa gcagaagttc gcgggcggcc tgtttgagga catgggccag
     1261 aggcggctgg ctcagggacg agccagcccg accgaggagg gacgaaagct ctgcttcagc
     1321 gctgtagatt ttctgccagg tccattcggt ccactggttg agccagatgt agtggtagcc
     1381 gccctcgccc caggaaccct ccgggatgga gataacagtt gatggtttgg cgagatcgag
     1441 gtattcaccg gcggtagaga gggaaatctc cgggtcctgg gcaatcttcg taagcacctt
     1501 tttcaaaaag cgcgggcctt cgaaccacca gtggccgaag agttcggcat cgtagggagc
     1561 gacgaccaat ccgggttttt tggtttgttc atagtaagcc agaatctcgc gcttaaccaa
     1621 cgaaacgaag tggttcgcat tttcttcaag tcgcgcttcc accgcttccg gttcatattc
     1681 ctttttgtcg gccaaatcgc ttttggcgct ggtgaccttc cagtaacgca ggccgcccgg
     1741 gaaatgtttt ttgtggaagt ccagataatt gccgtcggcg gggtagccgt gctcgcccga
     1801 ccagacgacc aaagcggttt ccgcatcgcg ggtgaaaaca gcgaccggtt ttttctccgg
     1861 taagccgacg aggtagactt cacggggcga gcggaggata ttttcttccg ccggccggta
     1921 ctccttttca aactgctgcc agagctgcgc caacgcctca aagcggtcga tgtagacccc
     1981 gatggctttg ccgcctttta gaagcgcggc gtcgatgaag aaaaactcga tcccgttttc
     2041 actcaaaaat tcttccacgc ccttgcgcgg gtgggcggtg caacccaaag gcgactcaac
     2101 cggcggggcc caggcataac cgggacggta ggcgcactcc ggcagccaga ggccgcgggg
     2161 ctttttgccg aaatgtctct ggtgcgcgac caccgcttgc tttacctgcg cttgcacact
     2221 catatcctgc gcgagaagcg ggaggtaacc gtgtgtcgca gcgcaggtga tcacctcgat
     2281 tgacccggtg tcgttcagct tgcgaaaagc accgatgagg tcatggttgt acttttcctc
     2341 aaaatctttc ttgattccca agtaaaagtc atgccacatg gctgccgtcg cggccagttg
     2401 gatcaatcct ttctgtttga aggcgggtcg gtcctgcttg gcggctttga ttttctggtc
     2461 gaggtaggtt aaaagttccg caacgaatgc atgggaagcg agttgttcac agagaacggg
     2521 tgaaagattg agggtcactt tgggggggat gcctgtggcg accaggtcgt tgaaaacatt
     2581 caaaagcggc aggtacgtct cgcaggccgc ctcggagagc caatcagttc cgtgcggcca
     2641 gcggccgtgc gccaaaacgt acggcaggtg cgcgtgcagg acgagggaga agtaaccttt
     2701 gggttgaggg gtcaccggat gctttgataa attggaatcg gttggtatga cgctttcccg
     2761 ctaagggccg gcctcccacc acgtaaggta gtcgggctcg tctctctgcc cgacggttct
     2821 cctcgaccgg ctcgggtcgg cacgactctt cgaggacgag cccctacggg tcgttcgacc
     2881 ggtctaccag agtaaccgct cataccggct ggtcggtccg aaccacaggg gtggcggagg
     2941 gttcgaccgt tttgaaaccg atgcccttat cttctttccc gaaaattttg atctggccga
     3001 attgcgcttc gtacttcttc aagttctcgt ccaacgtctg cagcaggagt ttggcgtgct
     3061 ggggcgtcat aatcacccgg gcgtagactt tggagcgcgg cacgccgggc aggagccggg
     3121 cgaagtcgat aatcacctcc gagggcgaat gggtgatgaa agcgaggttg gaataaatgc
     3181 cctccgattc cttttcgcct aattcgacgt taatctgctg cggctgcggt ttaatttcca
     3241 ttttgcctcc ggttcgtggg cggggattct cccgaccggc tcggagacga gccggtctac
     3301 cccgacttgg gtagtcgggc tcgtccccga gcccgacagg tctacgacgt gcaatcgttt
     3361 cactccgctt ccccgcgtag tcggcccgta cgggccgact gctggtctca tttgtgtttc
     3421 gttacctcac gaatatattc gtccatcctt gagggtcaag gtgaggaatt ttcgggagcg
     3481 gagatgtcca caataaagcg catcggcgtt ttgacctccg gcggggatgc gcccggcatg
     3541 aattcggcca tccgggcggt cgtccacgaa gcggtggaaa aggggctctc ggtcttcggt
     3601 ttttcccgcg gcttcaacgg gctgatcgat aatgaaggta tcgaactcga tctgaaggcg
     3661 gtgggcgggc tgctccagcg cggggggacg atgctccttt cggcgcgctc ggatgaattt
     3721 aaaacgcccg aaggccggaa gaccgcccgc cagagtctgg agaaacggaa agtcgatgcc
     3781 ttagtagtga ttggcgggga cggatcgttc aaaggcgcgc aacttttgga ggaagaaggg
     3841 gtggcggttg ccggggtgcc cgccaccatc gacaacgaca tctacggcac cgacatggcc
     3901 atcggcgtcg atacggcgct caacaccatc gtggaagtgg tggataaaat caaagacact
     3961 gcctcatcct tggagcggac ctttgtcatc gagacgatgg gccgcgactc cggctacctg
     4021 gcgttgatgt cggccatcgc caccggcgcg gaagccgcga ttattcccga agtcccctat
     4081 gatctggaaa aaatcgcccg gcgcctgtac gaccgctacc agcggggcgc gaccaacagc
     4141 atcatcatcg tggccgaagg ggcctccagc gcctacgaga tcagccgcaa gttagccaag
     4201 atcgcttcca tcgaaacgaa aatcaccgtc ttgggacacc tgcagcgggg cgggtcaccg
     4261 tcagctttcg accgcctcct gggcagccgg ctgggccccg cggcggtgga agcgctcctg
     4321 cggggcgagt cgggagtgat ggtggggctg gtcaagcggg gagt
//
LOCUS       DBNGZA010000226         3767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_43092, whole genome shotgun sequence.
ACCESSION   DBNGZA010000226 DBNGZA010000000
VERSION     DBNGZA010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_43092"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="AC16FU_04710"
     CDS             complement(<1..1031)
                     /locus_tag="AC16FU_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR2993709.1"
                     /translation="MSSIFAKKQLSNLLRSKFEFQGANTQYGSHSIHSFAAKFPPQLP
                     AAFILNLTEPGEIVLDPMAGSGTALVEALLAKRRCIGIDLDPLACLIADVKTRPAGSF
                     VSEQDIDRVIFNAVAALQHTALLDKIGEKRFDEKTRLFLDYWFLRPTQHELLSLLAAI
                     DEEQNPRAKQFFKVVFSSIIVTKSGGVSLARDLAHSRPHRDSEKPRQDAFELFRKKAV
                     KALRGLRIFEKPTHDALVMRGDVRSLPLQNNVIHLIVTSPPYANAIDYMRAHKFSLVW
                     LGYPIELLSTVRSSYIGAEKQNHHNGYYSSLANTVLKELDEVDQKRSKIVNQYFAEML
                     VALKEMYRVL"
     gene            complement(1058..1609)
                     /locus_tag="AC16FU_04715"
     CDS             complement(1058..1609)
                     /locus_tag="AC16FU_04715"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HIR2993710.1"
                     /translation="MVIIPKKIFLTKGVGKHREQLSSFELALRGAGIARFNLVSVSSI
                     FPPKCKVISKARGLQYLYDGQVVFCVIARNATNEPNRLISSSVGLAIPQDRRQFGYLS
                     EHHDYGMTEKKAADYAEDLAAEMLATTLGVGFDPNTSYDEKRQIWRISGKIVRTMNVT
                     QSAEGDKNGLWTTVVAAGIFITE"
     gene            complement(1845..3308)
                     /locus_tag="AC16FU_04720"
     CDS             complement(1845..3308)
                     /locus_tag="AC16FU_04720"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2993711.1"
                     /translation="MRKRRTFAGLLLLAGCLIFTTGAFAQPKISEGSKKIAQFKYKDI
                     TWQIPEVGKQVDTLTLPNGIKLFLLEDHAIPIFSMNALVRTGNIYEPADQMGLAGLTG
                     TVMRTGGTETLDPDSLNRLLEFIAGSVETGIGTESGVANLNVLSKDMNLGIEIFADVL
                     MHPAFRQEKIDLAKDQIRESIRRRNDSPGQIVSREFNHLIYGAHPYGRVLEWETVKKL
                     TRDDLIAFHRKYYKPNNILLGVAGDFKKAEVVAKLKQVFADWKPEPVDFPPLPKVADQ
                     PNPGVYVIAKEINQTNVTMGHLGCDFNNPDIYAISVMNFILGGGSFTSRMTSRVRSDE
                     GLAYSVGSSFNTGSRDLGTFSATCQTKTETTHKAISLMLEEIKKIREAPVTADELKTA
                     KDSFINRYVFQFTNPGAIVGQLMGLEYFNRPRDFLKTYLDKIRAVTAADVQRAAQKYL
                     KPENLTLVVVGKTDKFDKNLDDLGQVKPIALQPPAVD"
     gene            complement(3383..>3767)
                     /locus_tag="AC16FU_04725"
     CDS             complement(3383..>3767)
                     /locus_tag="AC16FU_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2993712.1"
                     /translation="YVQATPLAPHMPEEVEQAVYAEIERAKNEPVTDWELQKVKNQTD
                     AALVRSLQSNNGLAFVLGNTEAITGDWKNFITFAEKSKQVTPEQIQQVAKQYLTESNR
                     TVGILVQVKGKKVEPPKISPGSLKY"
BASE COUNT          788 a          984 c         1065 g          930 t
ORIGIN      
        1 aggacacgat acatttcctt taaggccaca agcatttctg cgaaatattg atttactatt
       61 ttacttcttt tctgatctac ttcatccagt tctttcagaa cggtattcgc caacgaggaa
      121 taatagccgt tgtggtgatt ttgtttctcg gcgccaatgt atgaactgcg gacagtagag
      181 aggagctcga tagggtaccc cagccagaca aggctaaatt tatgtgcacg catataatca
      241 atggcattgg cgtacggagg tgaagttact atgagatgaa taacgttatt ctgtagaggt
      301 aatgaacgga catcacctcg catcacgaga gcatcgtgag taggtttttc aaaaatacgc
      361 aggccacgta gggcctttac cgcctttttt cggaagagct cgaaggcatc ttggcggggt
      421 ttttcgctat cacgatgtgg gcgtgaatga gcgaggtcgc gagctaatga gacgcctccg
      481 gacttggtaa cgataattga ggaaaaaacc actttaaaaa actgtttcgc acgtgggttc
      541 tgctcctcat ctatggcagc caaaagactt aaaagttcgt gttgggtggg tctaagaaac
      601 cagtagtcga gaaataagcg ggttttctca tcaaaacgtt tttcacctat tttatccagc
      661 aaggctgtgt gctgtaaagc cgctacagcg ttaaaaatta cacgatcaat atcctgctca
      721 gagacgaatg agccagctgg tcttgttttt acgtcggcaa taaggcaagc cagagggtca
      781 aggtctattc cgatgcacct acgcttggcc agaagggctt caaccagtgc cgtccccgaa
      841 ccagccattg gatcaagtac gatttcaccg ggttcagtta agttcagtat gaaggctgct
      901 ggtaactgag gtggaaattt tgccgcaaac gaatggatac tgtgcgaccc atactgggta
      961 ttagccccct ggaattcgaa tttgctacgg agcaaatttg agagttgttt ttttgcaaaa
     1021 attgaactca agttagaatc ccttctttgt aatttagtta ctccgtaata aaaatccccg
     1081 ccgccaccac ggtggtccag aggccgtttt tgtccccctc cgccgactgg gtgacgttca
     1141 tcgtccgcac gatcttgcca gagatccgcc agatctggcg cttctcgtca tacgacgtat
     1201 tcgggtcgaa accgacgccc agagtcgtgg cgagcatctc ggcggccaag tcttcggcgt
     1261 agtcggcggc tttcttttcg gtcatgccgt agtcgtggtg ctccgaaagg tagccgaact
     1321 gccgccggtc ctggggaatg gccaacccca ccgaggagga gatgagccgg ttcggctcgt
     1381 tggtggcgtt gcgggcgatg acgcagaaga ccacctggcc gtcgtaaagg tactgcaggc
     1441 cgcgcgcttt ggagatgacc ttgcacttgg gcgggaaaat gctggagacc gaaaccaggt
     1501 tgaaacgggc gatgccggcg ccccgcaggg ccagctcaaa cgacgagagc tgctcccggt
     1561 gcttgccgac ccctttggtg aggaaaatct ttttgggaat gatgaccacg ctaccatgcc
     1621 tccgcttttt tcgacccgcc cgttttcaaa atcgggaaaa aatatacagt ctgtgcgccg
     1681 ggacgcaata aaaattgctt cggtcaagca ggtttttttg atgccgtgat ggtgtcggcg
     1741 cgccgcgggc cggagctcgg agaagaaacc taggaaggcc ggcggagaaa agacaacccc
     1801 gatctccgcc gcctaaggcg ggactcttgc gtttcgatcc gcaatcagtc cacggccggg
     1861 ggttgcaggg cgatcggctt cacctggccc aggtcgtcga gattcttgtc aaacttatcc
     1921 gtcttcccca ccacgacaag ggtcaagttc tccggcttca ggtacttctg ggccgcgcgc
     1981 tggacgtcgg ccgcggtgac ggcgcgtatc ttgtccaggt acgttttcaa aaagtcccgc
     2041 gggcggttaa agtactccaa ccccatcaac tggccgacga tcgctcccgg gttggtgaac
     2101 tgaaagacgt agcggttgat gaaggaatct ttggccgttt tcagttcgtc ggcggtgacc
     2161 ggcgcctccc ggattttttt gatttcctcc agcatcaggg agatggcttt gtgcgtcgtt
     2221 tcggttttgg tctggcaggt ggcggagaag gtgcccagat cgcgggagcc ggtgttgaag
     2281 ctggagccga ccgagtaggc caatccttcg tcggagcgca cccgggaggt catccgcgag
     2341 gtgaacgagc cgccgcccag gatgaagttc atcaccgata tggcgtaaat gtcgggatta
     2401 ttgaagtcgc accccaggtg gcccatggtc acgttcgtct ggttgatttc tttggcgatg
     2461 acgtagacgc cggggttcgg ctggtcggcc actttcggca ggggggggaa gtccaccggt
     2521 tccggtttcc agtcggcaaa gacctgtttc aacttcgcta ccacctcggc ttttttgaaa
     2581 tctccggcaa cacccaaaag aatgttgttc ggtttgtaat atttccggtg gaaggcgatc
     2641 aggtcatccc gggtcagttt cttgaccgtc tcccactcca acacgcggcc gtaggggtgt
     2701 gctccgtaaa tcaggtggtt gaactcgcgg gaaacgatct gccccggcga gtcgttgcgc
     2761 cgccggatcg attctctgat ctggtctttg gccaagtcga ttttttcctg ccggaaagcc
     2821 gggtgcatca atacgtcggc gaaaatctca atccctaagt tcatgtcctt ggacaaaacg
     2881 ttcaagttag cgacccccga ttcagtgccg atgccggtct ccaccgagcc ggcaatgaac
     2941 tccagaagcc ggttgagact atccgggtcg agcgtttcgg tgccgccggt gcgcatcacc
     3001 gtgccggtga ggccggccag ccccatttgg tccgccggtt cgtaaatgtt gccggtgcgg
     3061 acgagggcgt tcatcgaaaa aatcgggatg gcgtggtctt ccaagaggaa gagtttgatc
     3121 ccgttgggca gggtcagggt gtcgacctgc ttgcccacct ccggtatttg ccaggtgatg
     3181 tctttgtact tgaactgcgc gatttttttg ctgccctcag agattttcgg ctgggcaaaa
     3241 gcaccggtcg taaaaatcag gcaaccggcc aggagcaata ggcccgcaaa tgtgcgacgt
     3301 ttcctcacga tcgatcctcc tttttccgaa ctcaggttat gagttttcaa aatgttcccg
     3361 gttcttctcg gtcagcgcaa ggttaatact tgagcgagcc cgggctgatt ttgggcggtt
     3421 cgactttctt ccccttcacc tgtaccagga tccccaccgt acggttcgac tcggtcaaat
     3481 actgtttggc cacctgctgg atttgctcgg gggtcacctg tttggatttc tccgcgaagg
     3541 taataaagtt cttccaatcc ccggttatgg cttcggtgtt gcccagaacg aaggccaggc
     3601 cgttgttcga ctggagactg cgcaccaggg cggcatcggt ctggtttttc accttctgca
     3661 attcccagtc ggtgaccggc tcgtttttcg cccgctcgat ttctgcgtag accgcctgtt
     3721 ccacttcttc cggcatatgc ggggccaggg gcgtggcctg cacgtag
//
LOCUS       DBNGZA010000227         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1737377, whole genome shotgun sequence.
ACCESSION   DBNGZA010000227 DBNGZA010000000
VERSION     DBNGZA010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1737377"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC16FU_04730"
     CDS             <1..191
                     /locus_tag="AC16FU_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993713.1"
                     /translation="YELHISIETSDKKKDIGRDYRFTLFESDSNELSRSKDDYKYGDG
                     IYWDSGNHTGVVVQIVEA"
     gene            complement(389..844)
                     /locus_tag="AC16FU_04735"
     CDS             complement(389..844)
                     /locus_tag="AC16FU_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006990012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR2993714.1"
                     /translation="MQSKAKTVKEYLAELPPERREAIQGVREVILKNLPQGYEETMQY
                     GMIGYVVPHRLYPPGYHCDPKLPLPFACLASQKNYMAIYFMHIYGDREAEAWFRKAYQ
                     ASGKKLDMGKSCVRFKKLEDLPLEVIGKAAALTPVKKCIERYEKIRKGK"
     gene            complement(933..1046)
                     /locus_tag="AC16FU_04740"
     tRNA            complement(933..1046)
                     /locus_tag="AC16FU_04740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(971..973),aa:Gln,seq:ctg)
     gene            complement(1079..1891)
                     /locus_tag="AC16FU_04745"
     CDS             complement(1079..1891)
                     /locus_tag="AC16FU_04745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2993715.1"
                     /translation="MKLKIVLTVTVMSLFICGATAGFTGAFTHLTAGRFTLYTIEFIT
                     LEGTTLYGIFERRAPVFGRIFWRGSQNLHAIALTFDDGPNEPYTSGVLDILREFRVNA
                     TFFVIGENVERFPGVVRRTAAEGHELANHTYDHEVLPLRWPGPIRNQIKRTSDLIEKT
                     TGRRPGLFRAPHGWRNPWVNRIAREEGCVPVAWTVGVWDTDRPGAEVIVQRTLKGLGN
                     GCVLLLHDSRGTERGADASQLVTALPAILQEAQRQGYRFLTLSEMVGETNKK"
     gene            complement(1888..3024)
                     /locus_tag="AC16FU_04750"
     CDS             complement(1888..3024)
                     /locus_tag="AC16FU_04750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993716.1"
                     /translation="MALKIGIVSEYYYPLLGGITENVYNTRVRLQERGHEVKIITANC
                     DGTRFAVHRSQDFTDANVIRIGRSLPIYSNSSFGRFTAGRQLRAKMKGILERERFDLL
                     HLHSPVTSSLPAVALLEAKCALVGTFHSYFDWNLVYSVFKGFIQKKGINRLDGKIGVS
                     QSSIDALRRYFDLEARIIPNGVDTTQFSPAAPRLEKFSDGKRNLLFLGRFDPRNGLAL
                     MLRAFAITKAEFPDVRLIVVGDGTFRNYYRRLVPKEVAQDVHFAGPVRDERPAYYATC
                     DVFCSPVTKASFGITLLEAMAAGKPIVATDNIGYREVLSPEEGFCVPPDDPALFARAI
                     LRLLRDENLRQEMGANGRKKALRYSWDKVAGDIAGYYAEILKTR"
     gene            complement(3151..3224)
                     /locus_tag="AC16FU_04755"
     tRNA            complement(3151..3224)
                     /locus_tag="AC16FU_04755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3190..3192),aa:Gln,seq:ctg)
BASE COUNT          675 a          969 c          935 g          720 t
ORIGIN      
        1 aatatgaatt acatatctcg atcgagactt ccgacaaaaa gaaagatatc gggagggact
       61 ataggtttac tctctttgaa tcagattcga atgaactgtc aaggtctaaa gatgattaca
      121 aatatggtga tggaatatac tgggactctg gaaaccatac tggcgttgtt gtgcagattg
      181 ttgaggccta atcagcgagt gcaggctatg cccaccggcg gtgtctgcga ctgacccttg
      241 tccgttaggc tggcaagttc gacgaccgcg cgatcttgct cgcgttcgcg ggcaggattc
      301 gaaccgtcat tagaaagtcg gaaatggaat tggctggggg gacagggatt tcccggccgt
      361 aacccgagga ctcggctcgg gggaacctct attttccttt cctgattttt tcgtatcgct
      421 ctatgcattt tttgaccggg gtcaacgccg ccgccttgcc gatcacctcc agcggcaggt
      481 cctccaattt cttgaaccgg acgcacgatt ttcccatatc cagcttcttg ccgctcgcct
      541 gatacgcctt tctgaaccag gcctccgcct cccggtcgcc gtagatatgc ataaagtaaa
      601 tcgccatgta attcttttgc gaggcgagac aggcgaacgg cagcggcagt ttggggtcgc
      661 agtgataccc cggagggtag agccggtgcg ggacgacata gccgatcatc ccgtactgca
      721 tcgtttcctc atacccttga ggcagatttt tgagaatgac ttcgcggact ccctgaatcg
      781 cctcgcgtct ttccggcggc agttcagcca ggtactcttt tacggttttt gccttgcttt
      841 gcataagccc tcaataatgt agccggtttg actttgaccg gacaaggttt ttcactgatc
      901 cctgatctcc aatccctgat ctctgctctc aaagctgggg gacagggatt cgaaccccga
      961 ttaagtggtc cagagccact cgtcctgtgg tacgtatgac aaatgttaag actccacccg
     1021 tagggccgtt cgacgacttc cccagccaag ttacaaggcg tactttcctt cgtcaacttt
     1081 acttcttatt ggtctccccc accatttctg aaagcgtcag aaacctgtac ccctgtctct
     1141 gggcttcctg cagaatagcc ggcagcgccg ttactaattg actggcatcg gcgccgcgct
     1201 cggtgccgcg gctgtcgtgg agcaaaagga cgcagccgtt gcccagccct ttcagcgtgc
     1261 gctggacgat aacctccgcg ccgggacggt cagtgtccca cacccctacc gtccaggcga
     1321 cgggaacaca cccctcttct ctggcgatcc ggttcaccca gggatttcgc cagccatgcg
     1381 gcgcgcggaa gagtccggga cgccggccgg tggtcttttc gatcagatcg gaggttcttt
     1441 ttatctgatt gcggataggg cccggccagc gcaaagggag cacctcgtgg tcgtacgtat
     1501 ggttggcaag ctcatgccct tccgcagccg tccgcctgac cacgccgggg aatctctcga
     1561 cgttttctcc gatgacgaag aaagtcgcgt tgacccgaaa ctccctcaag atgtctaaaa
     1621 ccccggaagt gtacggctcg ttggggccgt cgtcgaaggt aagcgcaatg gcgtgcaaat
     1681 tctgagaccc ccgccagaag atgcgcccga aaaccggcgc ccgccgctcg aaaataccgt
     1741 aaagcgtcgt accttcgagg gtgataaact cgatggtata gagcgtgaaa cgtccggcgg
     1801 tgaggtgggt aaatgctccg gtgaaaccgg cggtggcccc acagatgaag agcgacatca
     1861 ccgttacggt gaggacgatc ttcagtttca tcgcgtcttt aaaatctcgg cgtagtaacc
     1921 ggcgatgtcg ccggcgacct tatcccacga ataccggagg gccttttttc gcccgttggc
     1981 tcccatttcc tgtctcagat tttcatcccg caggagacgg aggattgccc gggcgaatag
     2041 ggcggggtcg tccgggggca cgcagaaccc ctcttccggg ctcaacactt cccgataccc
     2101 gatgttgtcg gtggccacga tcggcttgcc cgcggccatc gcctccagaa gcgtgatgcc
     2161 gaaagaggcc ttggtgaccg gagaacagaa gacgtcgcag gtcgcatagt acgccgggcg
     2221 ttcatcccga accggtcccg caaaatggac gtcctgcgcc acttccttcg gaaccagtct
     2281 ccggtagtag ttccggaacg tgccgtcccc gacgacgatg agccgcacgt cggggaattc
     2341 ggctttcgtg atcgcaaacg cccgcagcat caaagccagg ccgttccgcg gatcgaaccg
     2401 gcccaaaaag aggaggttcc gcttgccgtc gctgaacttc tccagccgcg gcgccgcggg
     2461 gctgaactgg gtggtatcca cgccgttcgg tataatgcgc gcctcgaggt cgaaataccg
     2521 gcgcagcgca tcgatgctgc tctgggaaac gccgatcttt ccgtccagcc ggttgatgcc
     2581 ttttttctgg atgaatccct tgaagaccga atagaccagg ttccagtcga agtagctgtg
     2641 aaacgtaccc accagtgcgc acttggcctc cagcagcgcc accgccggga gagaagaggt
     2701 gacgggggag tgcaggtgga gtaagtcgaa ccgctcccgc tccaggatac ctttcatttt
     2761 tgcccgcaac tgcctgccgg cggtgaagcg gccgaaggaa ctgttggagt agatcggcag
     2821 actgcggccg atgcgaatga cgttcgcatc ggtgaaatcc tggctccggt ggacggcgaa
     2881 ccgcgtgccg tcgcagttgg cggtgatgat tttcacttcg tggccgcgct cctgcagccg
     2941 cacccgcgtg ttgtagacgt tctcggtgat gccccccaga agcgggtagt agtattcgct
     3001 gacgatccct atcttcaaag ccacggttgg cgttttctcc ccgggtgctc gcgacctgcg
     3061 tcagtcaacc ggcgggctcg acggcacgac cttccctgtc acccacagaa aatcttccgg
     3121 cactacggcc aaaggaaaaa aaatgaaaat tggctggggg acagggattc gaaccccgat
     3181 taagtggtcc agagccactc gtcctgccgt tagacgatcc cccaggtcga gtaaaaatca
     3241 cttgccagag tatacggttc gacgacgctc gccgtcaacg gcttaggcgg cgccggtca
//
LOCUS       DBNGZA010000228         7645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1421771, whole genome shotgun sequence.
ACCESSION   DBNGZA010000228 DBNGZA010000000
VERSION     DBNGZA010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1421771"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC16FU_04760"
     CDS             complement(<1..457)
                     /locus_tag="AC16FU_04760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR2993717.1"
                     /translation="MKKRSKATEKLYVTSVATRMGTVHLASTPRGVARIALPGENHKA
                     FFSWLVKTFPKAEISTQGRVNEKPARQLREYFEGKRKKFNFRLDLRSTPFQSRVLRKL
                     RAIPYGKTASYGEIAKKIGRSKAFRAVGLACGSNPLPVVIPCHRVIGANG"
     gene            complement(482..1408)
                     /locus_tag="AC16FU_04765"
     CDS             complement(482..1408)
                     /locus_tag="AC16FU_04765"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR2993718.1"
                     /translation="MSSITLRPVRPYRFDLILHHWGHNPHEILDVVKNGAFHRVIELE
                     STPVLYRLINSGTNRKPQLSLRFLNGRLSRTQLEKATRIVSRTFSLHVDLKKFYRAVA
                     GDEVLSRIGKTFRGLKPKLIGSTLFENLAIAIMGQQVNLTFATTLKRRLVEAFGPRVV
                     YRGKIFYGFPAAGRLATVDPEKLRKMQFSRQKSRYILDLARQVASGEIDLESYRYLEP
                     DRAVEQLMAIRGIGRWTAEYCLMRGIGYLDALPAGDTGLQNAVTRFYRFKKRATEDQV
                     RKLGERWKPYRSLATFYLWYALSKRLPLIPKS"
     gene            complement(1427..2437)
                     /gene="tsaD"
                     /locus_tag="AC16FU_04770"
     CDS             complement(1427..2437)
                     /gene="tsaD"
                     /locus_tag="AC16FU_04770"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR2993719.1"
                     /translation="MLLLGIETSCDETAAGVLKDRRTLLSNVILSQVVHQPYGGVVPE
                     LASREHMRNLVPIVDQALAEAKTPLEKIEGLAVTFGPGLVGALLVGISFAKAVAVSRR
                     LPIIGVNHLEGHIFSNALEHPELEPPFVCLVVSGGHTLLVLVKNWGVYRLLGQTRDDA
                     AGEAFDKVAKLLGAGYPGGQKIDDLAQTGNPARITFPRAYLEKEAYDFSFSGLKTAVA
                     LYVQKLSERERENLLPHLCAGFQEAVVDVLVAKTVRAAEESNVSAIAVAGGVARNRRL
                     RQRLGDEASRRGMKFFVPVPQFCTDNAAMIAAAGAFHLSRGERSDLSLNAAPYVPLNG
                     LA"
     gene            complement(2437..3354)
                     /locus_tag="AC16FU_04775"
     CDS             complement(2437..3354)
                     /locus_tag="AC16FU_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR2993720.1"
                     /translation="MVKPLWENLPVKVAALLLAVILWFHVSTERRVEHSLAVPLTYTG
                     LDSSLVFLKLPPPAVEVRVRGTGKAVLKAIWQKKAVPVKLDLLGPGNYDRLVRQADLR
                     QALDPELEILELIQPKSLTFSLDRVEEKTVKIESRVTVEPALGYFWNGELTFEPVLAT
                     VRGPQQALAKIEKVFTDTAHLRGTQTPLVATLRLLAPDSFNVSLVTPTVIVRAPVEKV
                     SSLVLDSIPVKTLISALQRVAWIRPNLLRLTLEGPESALRKIDRKTLKAVVNCSRLKA
                     GRHWLVPQVSVPPPVVVDAPLDSVEVRIE"
     gene            3420..4550
                     /locus_tag="AC16FU_04780"
     CDS             3420..4550
                     /locus_tag="AC16FU_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993721.1"
                     /translation="MAQVRFDRVTKIFDSQQPALTNFFLEVKDGEFVVLVGPSGCGKT
                     TALRLLAGLEEPTAGDLFIGERRVNTIPPKDRDVAMVFQNYALYPHMTVYENLAFPLK
                     VRHIVKNEIDRRVRETAELLGLAPLLSRKPKALSGGERQRVAVGRALVRQPQVFLFDE
                     PLSNLDAALRSQLRGELLHLKTKLGATALYVTHDQVEALTLGDRVAVLKAGELQQVGS
                     PGELYRRPQNLFVATFIGTPPMNLIRARVFRQDGLFCHEVFRWRVPPPQAEILEAYVG
                     REIILGLRPDILRVVATDADASGMERFPISIDFMEPIGGEMLLHGTAAGTPVIITERR
                     VMGERKVSPGEKITCAFEWRQVHLFDPASGQRISFPTLTVPP"
     gene            complement(4534..5454)
                     /locus_tag="AC16FU_04785"
     CDS             complement(4534..5454)
                     /locus_tag="AC16FU_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993722.1"
                     /translation="MAVLDFWRRDRFHWRSSLLSIFPFTLIAVASFLYRLPALLAVDS
                     TAAYVPVIGLHILKNYLFLFTGSLVDLDFRHLLEIWNAFRDSHNFSQLLTQITRMGVI
                     PAIIVVMFLYILLWWRGGKAVRLMLLWAILFFLPGSLLKGTGERILYLPLFGLVTAWV
                     TFLLPLLRKFWSTARTQRLAAGTVLALFLLWVGYNVFRLHTRVGCWEKAGAISENVIQ
                     TIDRMQSNWPPGTTVLALDILDNYEGAWVFRMGFEQLPYLFWPTKHFSFAKADSNSLS
                     QLIRENKNFGRPVRVLRYTNETVVEVTAAR"
     gene            complement(6091..6813)
                     /locus_tag="AC16FU_04790"
     CDS             complement(6091..6813)
                     /locus_tag="AC16FU_04790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2993723.1"
                     /translation="MTSNRTLTQLADFVGSENLFLQKTPPAVCPRAAAEVKSIVAFAC
                     GRKIPLAIQGNGAHLPVSDPNRVVVSLERLNRVVELASDDLYVTLEAGFPLGVLNQTL
                     SPHKVAYPLSQAGYNGTVGGSVAMNLSATLEDDTIQTKTYLMALEAVTGYGELVKLGA
                     KTYKSVAGYDVARFLCGSWGRLAVITKVTLRLIPLRLQKQYEKARLIDPIWPWGQAKR
                     DPALAALENQVKSQVDPTGILP"
     gene            complement(6822..>7645)
                     /locus_tag="AC16FU_04795"
     CDS             complement(6822..>7645)
                     /locus_tag="AC16FU_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HIR2993724.1"
                     /translation="KFGAFRSSGFNRISMGYQSIFDHHLKILGRDHTAAEAERACLWA
                     RRAGFKNISLDFIYALSDQTLAEWEQTLARAIALSPEHISAYCLTIETRTPYALYFQQ
                     GRLHLPDEEISRQMFAAARILLAEAGYEHYETSNWAKPGFRSRHNEKYWTDREYLGFG
                     PSAHSFLRNQRWANVRDLKVYTARIVEGKLPRDFIETLSPVEERTEAILLGLRRAEGI
                     DLENYALRFGTRLELDKQKEIERFIRLGYLKRTDGHLQLQAEGWFVADGIMAALA"
BASE COUNT         1753 a         2066 c         2256 g         1570 t
ORIGIN      
        1 atccatttgc gccaatcacc cggtggcagg gaatgaccac cggcaaagga ttggagccgc
       61 aggccaggcc gacggcacgg aaagccttcg agcggccaat ttttttggcg atttcgccgt
      121 aactggcggt tttgccgtag ggaatcgcgc gcagtttccg caacacccgg ctctggaacg
      181 gggtcgagcg caggtcgagc cggaagttaa acttcttccg tttcccttcg aagtattccc
      241 gcaactgccg ggccggcttt tcattcaccc gtccctgggt cgagatttcg gctttgggaa
      301 acgtcttgac cagccacgaa aagaaggctt tgtggttttc acccggcagg gcaatgcggg
      361 caacgccgcg cggggtggaa gcgagatgca cggtccccat ccgcgtggcg acgctggtga
      421 cgtagagttt ttccgttgct ttcgagcgct ttttcataaa gctcctttca ggatttagga
      481 gttaggattt gggaattaga ggtaatcgct tcgacagagc ataccacagg taaaaggttg
      541 ccagactacg gtacggtttc cagcgttcgc ccagcttgcg cacttggtct tcggtggcgc
      601 gcttcttaaa ccggtaaaaa cgggtgaccg cattctgcag gccggtgtcg ccggccggca
      661 gggcatcaag ataaccaatt ccccgcatta agcaatactc ggcagtccag cggccgatgc
      721 cgcgaatcgc catcaattgc tctaccgccc ggtcgggttc gagataccgg tacgattcca
      781 ggtcgatttc acccgaagcc acctgccggg ccaggtcgag aatgtagcgg ctcttctggc
      841 gggagaattg catcttccgc aatttttcgg ggtcaactgt ggcgagccgc ccggcagccg
      901 gaaaaccgta gaaaatcttt ccgcgataaa ccacccgcgg gccgaaagct tcgaccagtc
      961 gccgcttgag cgtcgtggca aaggtcagat tgacctgctg gcccataatg gcaatggcca
     1021 ggttctcgaa aagggtcgag ccgatcagtt tcggtttgag accgcgaaac gttttgccaa
     1081 tgcgcgataa aacttcatct ccggcgacgg cgcgatagaa ctttttcagg tcaacgtgca
     1141 gagaaaaagt gcgggagaca atccgggtcg ctttttctaa ttgtgttcga gacaacctcc
     1201 cgttgagaaa gcggagcgag agttgcggct ttcggttggt tcccgagttg atgagtcggt
     1261 agagaaccgg cgtgctctcc agttcaatga cccggtggaa ggcgccattc ttcaccacgt
     1321 ccagaatttc gtgggggtta tgaccccagt ggtggagaat caggtcaaag cggtagggcc
     1381 ggactggtcg aagcgtaatg gaagacaaag gcaaactgtt gcggttttag gccaagccat
     1441 tcaacgggac atacggtgcc gcattgagag acaaatccga tctttcacca cgggacagat
     1501 ggaaagcgcc ggcggcggca atcattgccg cattgtcggt gcagaactgg gggacaggta
     1561 cgaagaactt catacctcgt cggctcgctt cgtcaccgag cctttgtctg agtctccggt
     1621 tccgcgccac gccgccggcc acggcgatgg cggaaacgtt cgattcttca gccgcgcgca
     1681 ccgttttcgc aaccagaaca tccaccaccg cttcctggaa accggcgcag aggtggggca
     1741 agagattctc ccgttctctc tcggacaatt tttgcacgta gagggcgacg gcagttttga
     1801 gaccgctgaa actgaaatcg tatgcttcct tttccaggta ggcgcgcggg aaggtgattc
     1861 tggccggatt gccggtctgg gccaaatcgt caatcttctg cccgcccggg tagcccgcac
     1921 ccagaagttt cgccactttg tcaaacgctt cgccggcggc atcgtcacga gtttgcccca
     1981 gaagtcggta tacgccccag tttttcacca gcaccaaaag cgtgtgcccc ccggagacga
     2041 ccagacagac gaacggtggc tccagttccg ggtgctccag ggcattggag aaaatgtgcc
     2101 cttccaagtg gttcacgccg ataatcggga ggcggcgcga caccgccacc gccttggcaa
     2161 acgaaatccc caccaaaagc gccccgacca gcccgggacc gaaagtgaca gccagcccct
     2221 ctattttttc cagaggagtt ttagcttcgg ccagcgcctg gtcaacgatc ggcaccaggt
     2281 tgcgcatatg ttcgcgggag gccagttccg gcaccacgcc gccgtagggc tggtgcacca
     2341 cctgcgagag gatgacgttg gacagaagcg tccgccgatc tttgagaacc ccggcggcgg
     2401 tctcgtcaca agacgtttcg atacctaaaa gcagcactac tcaatccgca cttccaccga
     2461 gtcgagaggg gcgtccacga ccaccggagg cggtacgctg acctgaggga cgagccagtg
     2521 tcggcccgct ttgagcctgg agcagttcac caccgccttg agcgtcttgc ggtcgatttt
     2581 tcggagggcg ctttcggggc cttccaaagt caggcgcagg aggttcggcc tgatccaagc
     2641 tacccgctgc agggccgaga tgagcgtctt gaccggaatc gaatcgagga cgagactgct
     2701 cactttctcg actggtgcac ggacgatgac cgtcggcgtt acgagcgaaa cgttaaagct
     2761 atcgggggcc agaagcctga gggtggccac caaaggcgtt tgggtgcccc gcaggtgggc
     2821 ggtatcggtg aacacctttt cgattttggc gagggcctgc tgcgggccgc ggacggtggc
     2881 cagcacaggt tcaaaagtga gttcgccgtt ccagaaatag cccaaggccg gttcgacggt
     2941 cacccggctt tcgatcttaa cggttttctc ctcgacgcga tccaaggaga aggtcagcga
     3001 cttcggttgg atcaattcca agatttccag ttccggatcc agcgcctgcc gcagatcggc
     3061 ctgccgcacc aggcggtcgt aattgccggg gcccaagagg tcgagtttca ccggcaccgc
     3121 tttcttctgc cagatggcct tgagcaccgc tttgcccgtg ccgcgcaccc gcacctccac
     3181 ggcgggaggc ggcaacttga ggaagaccag actcgaatcg agccccgtgt aggtcaaagg
     3241 caccgccaga gagtgttcca cccgtctttc ggtggagacg tgaaaccaaa ggatcaccgc
     3301 caagagcagg gcggcaactt tcaccggcag gttttcccac aggggtttga ccatcacgac
     3361 gattatacct gctcgattta cggttgacaa cccgcctgcg gcggatcatt ctatccgtaa
     3421 tggcgcaggt caggtttgac cgggtcacca aaatttttga cagtcagcaa cccgcactca
     3481 cgaatttctt tctggaagtc aaggacggcg aatttgtagt tctggtcggg ccttcgggtt
     3541 gcgggaaaac gacggctttg cgtcttttgg ccggtttgga agagccgacc gcaggtgacc
     3601 tgttcatcgg tgagcgccgc gtcaacacaa taccgcccaa agaccgggac gtggcgatgg
     3661 tttttcaaaa ctacgccctc tacccccaca tgacggtcta tgaaaacttg gctttcccct
     3721 tgaaagtccg gcacatcgta aaaaatgaga ttgaccggcg ggtgcgggaa actgccgaat
     3781 tgctggggct cgccccgctt ctctcccgca aacccaaagc gctctccggc ggcgaaaggc
     3841 agcgggtggc ggtggggcgg gccctggtgc gccagccgca ggtctttctc tttgacgagc
     3901 cgctttccaa tctggacgcg gctctgcgga gtcagttgcg gggagagctt ttgcatttga
     3961 agaccaaatt gggtgccacg gcgctctacg tcacgcacga tcaagtagaa gcacttacgc
     4021 tgggcgaccg ggtagcggtg ctcaaggccg gtgaattgca gcaggtaggc agtcccggag
     4081 agctgtaccg gaggccgcag aacctttttg tggccacttt catcggcacg ccgccaatga
     4141 atttgattcg ggcaagagtg ttccgacagg acggactttt ctgccacgag gttttccggt
     4201 ggcgcgtccc tcctccacaa gcagaaattt tggaagccta cgttggtcgg gaaatcattt
     4261 tgggactgcg ccccgatatt ctccgagttg tggcgacaga cgctgacgcc agcgggatgg
     4321 agcgttttcc gatttccatc gattttatgg aaccgatcgg cggcgaaatg ctcttgcacg
     4381 ggacagcggc cgggacgccg gtgatcataa ccgagcggcg ggtaatggga gagcggaagg
     4441 tgtcaccggg agagaaaatt acgtgcgcgt ttgagtggag gcaggtgcac ctatttgatc
     4501 cggcatcggg tcagcggatt tcgtttccga cactcaccgt gccgccgtaa cttccactac
     4561 cgtctcgttc gtatagcgca agacgcgcac tggtctccca aaatttttgt tttctcttat
     4621 cagttgtgat agggagttcg aatccgcttt agcgaaagag aagtgttttg ttggccagaa
     4681 gaggtaaggc aactgctcaa agcccatccg gaaaacccaa gccccctcgt agttgtccag
     4741 gatgtcaagc gccagcaccg tcgttccggg aggccagttg ctttgcatac ggtcgatagt
     4801 ttggatgaca ttttcactga tcgctccagc tttttcccaa cagccaacgc gggtatggag
     4861 ccggaaaacg ttatacccaa cccagagcaa gaacaaagca agaacagttc ctgcggccaa
     4921 ccgttgagtt ctcgcagtcg accaaaattt tctcagcaga ggtaggagaa aggtaaccca
     4981 tgctgttacc agaccgaaga ggggcaagta gagaatgcgt tccccggttc ctttcaaaag
     5041 agaacccggt agaaaaaaca gaatagccca aaggagcatc aaccgtaccg ccttcccgcc
     5101 acgccaccag agaagaatgt agagaaacat taccacgatg attgccggaa tcactcccat
     5161 ccgggttatc tgcgtgagca gttgagaaaa attgtgtgag tcacggaaag cattccagat
     5221 ttccaacaaa tggcggaagt ccagatcaac caatgagcca gtgaaaagaa aaaggtagtt
     5281 tttcaggatg tgcagtccga taacagggac gtaggcggcc gtcgaatcga cggcgaggag
     5341 ggcaggcagg cgatacaaaa agctcgcgac agcaatcaga gtaaacggga aaatggatag
     5401 gagacttgac cgccaatgaa aacggtcccg tcgccaaaaa tctaagaccg ccattaccgg
     5461 gacaaccata agcgcttcct ctttgcttga gagcgctgcc aagagcagga acccagtggt
     5521 aaatccggaa aggagcgttt tccggcgagc gtaccggaga tagatgagga gagtcaacag
     5581 ggtaaaggta gtgacgatga gagcggtgcg gccggacatc caggaaattg cctcactgtg
     5641 gataaagtga atactataaa gtccggcgga gagaagagcc agagcgggcc ggtgcgtcaa
     5701 acgaaccacc agccagtaac cgaggaggca attgatcacg tgcagcaaca aatttactcc
     5761 gtgccacgcg ataggactcc acccggtaag atgaaaattc aggtaacaga tgaggaaaat
     5821 cacggggcgg tataaactgg ttggtcggga aaagttttgt agaacgtcag taaaagacat
     5881 tgttgaagaa agacgcagaa accaaaaatc gtcggagaga aactggtggt tgaggttgcg
     5941 gccgtaggtg aacgccaaaa ccaagatgac gaaaacgata aagccagcgg ccagccagaa
     6001 gcgtgctttg gggcgcgaac tggtggcagg gatttcaact ctcatggaaa agagaattta
     6061 caaggatttg gccggaagca gaaacggttg ttacgggaga attccggtgg ggtcgacctg
     6121 acttttgacc tgattttcca gtgcggcaag ggcgggatcg cgctttgctt gaccccaggg
     6181 ccaaatcggg tcgatcagtc ttgccttttc gtactgtttc tgtaagcgca acgggatcaa
     6241 gcgaagcgtc actttggtaa tcaccgccag gcggccccag ctgccgcaga ggaagcgggc
     6301 cacgtcgtac ccggccacgc ttttgtaggt tttggcaccc agtttgacca gttcaccgta
     6361 gccggtcacc gcctccagcg ccataaggta ggttttggtc tggatcgtat cgtcttctaa
     6421 tgttgccgaa agattcatcg ctacgctccc gccgactgta ccgttgtatc ccgcctgact
     6481 cagcggatag gcaactttgt gcggcgatag agtctgattc aaaaccccta agggaaaacc
     6541 ggcctctaga gtgacataga gatcgtcgga agccagttcc accacgcggt tgaggcgctc
     6601 gaggctgacc acgactcgat ttggatccga taccggcaga tgagcgccat ttccctgaat
     6661 ggccagagga attttcctcc cgcaggcaaa agcgacgata gatttgacct cagctgccgc
     6721 gcgggggcag acggcgggcg gagttttttg caggaaaaga ttttccgagc caacaaagtc
     6781 ggccagctgg gtcaatgtgc gatttgaggt caagggtgtg gtcacgccaa ggccgccata
     6841 atgccgtcgg ccacaaacca gccctcggct tgtaactgga gatggccgtc ggtgcgcttg
     6901 aggtatccca ggcggatgaa acgctcaatt tctttctgct tgtctaattc gagccgcgtt
     6961 ccaaaacgaa gggcgtagtt ttccaagtca attccctcag cacggcgcag acccaaaagg
     7021 attgcctcgg tccgttcctc gaccggcgaa agcgtttcga taaagtcgcg gggcaattta
     7081 ccctcgacaa ttcgagcggt atagaccttc agatcgcgga cattcgccca gcgttgattc
     7141 cggaggaatg aatgggctga agggccaaag cccaggtact cccggtcggt ccagtatttt
     7201 tcattgtgcc gggagcggaa gccgggcttg gcccagttgg atgtttcgta gtgttcgtag
     7261 ccggcctcgg ccaagagtat ccgagcggcg gcgaacatct ggcgggaaat ctcctcgtcg
     7321 ggcaaatgca aacgtccctg ctggaaataa agagcgtaag gagtgcgggt ttcaatcgtc
     7381 aagcagtaag cggagatgtg ctcaggtgaa agtgcgatcg ctctggccag cgtttgttcc
     7441 cattcagcca gagtttgatc cgacagagcg tagatgaaat cgagactaat gttcttgaac
     7501 ccggcgcggc gggcccaaag acaggcccgc tccgcctcag cggcggtgtg gtcgcggcct
     7561 aaaatcttca ggtggtggtc gaagatgctc tggtagccca ttgaaattcg gttgaaaccc
     7621 gaggaacgaa aagcaccgaa tttct
//
LOCUS       DBNGZA010000229         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2800540, whole genome shotgun sequence.
ACCESSION   DBNGZA010000229 DBNGZA010000000
VERSION     DBNGZA010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2800540"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1788)
                     /locus_tag="AC16FU_04800"
     CDS             complement(70..1788)
                     /locus_tag="AC16FU_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993725.1"
                     /translation="MGTTFSDTQGNASDGSKHIVVDPTGAAHTAWHDTEAGGRPAAAR
                     GTYWTCVTAAGGVVNLGGAQIPSPQTAANYVSYRFVAVDATPDGRTLLAGHRSATAGT
                     PAPCAFGTTLNYNGWMGVDLGACLNSWVMSVQSCMDTSLNLVFADDATTADSTLHVTW
                     IDARQSSGPSPLCYSRSTDGGATWSVPSLLTGTGPSANSAGPGRDTLAPGSYFPGTRG
                     GSDRHMAFLSYRLIGSRKSRQLALVYGVAGAPEQPPGTQFSGNIWIRRSTDAGLSWEE
                     AKALTCFDSTSQFTAQNNQYSNMVSAIFDGNDSLHITAVFDEYDPVAQTAAFTGGVLA
                     HINAENPCVIHYITRGDFLNDPSLFGDTFGGSITHPSLATPGILDASKKNVLYAVWTQ
                     FSGSDVASQNDTSDFPFYNGEIYAAVSTDKGRSWSSRNLTQTKTPFCSPGTCASEDHA
                     WVADIVANDSLNIYYHLDKYGGQLSGGTTLYPPGNEVHLLRVSVADLPSGNALKGDQN
                     LDGVLDIVDIVTLVNCVVFGGGPNCLFPCLPDFNNDTLFDINDIAALINFVVFGNPPD
                     GFGGCG"
     gene            complement(2140..>2922)
                     /locus_tag="AC16FU_04805"
     CDS             complement(2140..>2922)
                     /locus_tag="AC16FU_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR2993726.1"
                     /translation="GVPWAKAGVGAVATQALANLAYGDDGLKMMAAGLSAELTLQKLL
                     EFDTLREERQVGLVDAKGGAATFTGKNCFNWAGGKTGKYYACQGNILTGPDVVDSMSA
                     AYERTAGRPLAERLLTALAAGQKVGGDRRGRESAAILVVKEKGGYGGYGDRWLDLRVD
                     DHPDPVKELLRLYYDVHQLYFGETDTTKLHKVDKALCTEIQTVLKKLGYYKGEVNGAY
                     DAATRRAFFDWQGWENLEMRWREDDRMDDVVLKYMREHYGRK"
BASE COUNT          601 a          855 c          848 g          618 t
ORIGIN      
        1 cggagtaagg aaagagcggt gggtagctaa acccaccgct ctttctagac cttctaacat
       61 ctcactttat cacccgcacc ctccaaaacc gtccggagga ttcccgaaca ccacgaaatt
      121 gatcagcgca gcaatatcat tgatatcgaa gagtgtgtcg ttgttgaagt caggtaggca
      181 ggggaataga cagttgggtc cccccccgaa cactacacaa ttaacgagcg tcacgatatc
      241 gacgatgtcg agcactccat caagattttg gtccccctta agggcgttcc ccgatggaag
      301 gtcggccact gacacccgca acaggtggac ttcgtttccc ggcgggtaga gcgtggttcc
      361 gccggagagc tgaccaccgt acttgtcgag gtggtaataa atattgagtg agtcgttggc
      421 cacgatgtcg gccacccaag cgtggtcttc actggcacag gtacccggac tgcagaacgg
      481 agttttagtt tgcgtaagat tgcggctact ccagctcctg cccttatcgg tggagacggc
      541 ggcgtagatt tctccgttgt aaaaggggaa gtcgctagtg tcgttctggc tggccacgtc
      601 ggatccagag aattgggtcc aaacggcgta gaggacattt tttttggacg catccaaaat
      661 tcccggagtg gctaagctgg ggtgggtgat gctaccgccg aaagtgtccc caaagagact
      721 gggatcattc agaaagtcgc ctctggtgat gtaatgaatg acgcagggat tctctgcgtt
      781 gatgtgtgcc aacacaccac cggtgaaggc ggcggtctgc gccacggggt cgtattcatc
      841 gaagaccgcg gtgatatgga gactgtcatt gccgtcgaaa atcgccgaaa ccatgttgga
      901 gtactggttg ttctgggcgg tgaactggga agtcgagtca aaacaggtga gggctttggc
      961 ctcttcccag gacaacccgg catcggtcga ccggcggatc cagatgttgc cggagaactg
     1021 cgttcctggc ggctgctccg gggcaccggc cacgccgtag accaaggcca gttgtctgga
     1081 cttgcgggaa ccgatcagcc tgtaactcaa aaatgccata tggcggtccg acccaccccg
     1141 cgtacccggg aagtacgaac cgggagcgag agtgtcccga cccgggccag ccgagttagc
     1201 cgaaggaccg gtgccggtca atagcgacgg gacgctccag gtagccccac catcggtcga
     1261 acgtgaatag cacaagggcg acggcccgga ggactgccgg gcatcgatcc acgtcacgtg
     1321 gagtgtgctg tcagcagtgg tggcgtcgtc ggcgaaaacc aggttcagac tggtgtccat
     1381 gcagctctgg acgctcatta cccaggagtt caaacaggca cccaggtcaa cccccatcca
     1441 accgttgtag tttagtgtgg tgccaaaagc acagggagct ggcgtcccgg ctgttgctga
     1501 ccggtgaccg gccaaaagcg tccggccgtc cggcgtcgcg tccaccgcca caaagcggta
     1561 ggaaacgtaa ttggcggccg tttggggtga cggaatctga gcaccgccca ggttgaccac
     1621 ccctccggcg gcggtaacgc aggtccagta ggtcccgcgg gccgctgccg gccggccgcc
     1681 ggcctcggtg tcgtgccagg cggtgtgagc cgcaccggtg gggtcgacaa caatgtgctt
     1741 ggaaccgtca ctggcgttac cttgcgtatc ggagaaggtg gtgcccaaaa aacggtcagt
     1801 tcctatagca ggggctggaa tgggtgcata ccgggaggcg ccgatatatc ggggaggccg
     1861 cctctttgcg gccgaaaagt aagtggtgtc cgactcgtcg gaacttactg tacgaagctg
     1921 atgttcctgg gcttgttttt tgggagtggg tagatcgctc ttggccttaa ctctgggcaa
     1981 cgacacgccc ccgaaaaaaa ccgcccagac cgctacaaaa aagacctttt tacccatctt
     2041 tgtgccctcc tttcccttgg gggttgaaca agggcacgca aaacggacag tttactctat
     2101 taatttatca gaatccttaa tatttgtcaa gttctttcgt cattttctcc cgtaatgctc
     2161 ccgcatgtac ttgagcacca cgtcatccat ccggtcgtct tcccgccagc gcatttcaag
     2221 gttttcccag ccctgccagt caaagaacgc ccgcctggtg gcggcatcgt aggcgccgtt
     2281 cacctcccct ttgtagtagc ccagtttttt gagcacggtc tggatttcgg tgcagagggc
     2341 tttgtcgact ttgtgcaatt tcgtcgtatc ggtttcgccg aagtacaact gatgcacgtc
     2401 gtagtacagc cgcagaagtt ctttgaccgg atcggggtgg tcatcaaccc gcaaatcgag
     2461 ccagcggtcg ccatagccgc cgtagccgcc cttttctttc acaaccaaga tcgctgccga
     2521 ctctctcccc cgccggtcgc ccccgacttt ttgaccggct gccaaagccg tcaaaagacg
     2581 ctcggccaaa ggtcggccgg cagtgcgctc atacgccgcg ctcatactgt ccaccacgtc
     2641 gggcccggtg aggatgttgc cctgacaggc gtagtatttg cccgtcttcc cgcccgccca
     2701 gttaaagcag tttttgccgg tgaaggtcgc cgccccgcct ttggcatcga ccaaccccac
     2761 ctgcctttct tcgcgcaggg tgtcgaactc gagtaacttc tgcagcgtca attccgccga
     2821 aagcccggcc gccatcattt tcagcccgtc gtcaccgtaa gccagattcg ccagggcttg
     2881 agtagcgacg gcgccgacac ccgctttggc ccagggcacg cc
//
LOCUS       DBNGZA010000230         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_360765, whole genome shotgun sequence.
ACCESSION   DBNGZA010000230 DBNGZA010000000
VERSION     DBNGZA010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_360765"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1998
                     /locus_tag="AC16FU_04810"
     CDS             <1..>1998
                     /locus_tag="AC16FU_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2993727.1"
                     /translation="LKVVVSTKDRRAFDGPIERGQFDLAILIYEKFNQLLIRNLDLLS
                     LIDLLVIDEIQMLDDPTRGPVLELALLKVLSFAKRPQIVALSAVLEDAAPLADWLGSR
                     LLVERKRPVELFTGVLLESRFAFRAFNTKEEGEEQWEVESAAGEDKIDADQRARLVTQ
                     IQKCVAGGDPVLVFLKGKRDCQGLAQLLAEAAPWPPAAGALEEIEAEPEVVLSESLAY
                     CLRRGVAFHHADLPFSLRRIVEHSFLAGEIKIVCATTTLALGVNLPAQTVFVEPWKYR
                     NGPQTNRPVLVPLSVAEFENMAGRAGRLNLAPSGRAVLLAKTPLEQEVLWSNYVTGRR
                     EKIASRLSHLPLGDIVLDLVAAESVKTVEELEATLLKSFHWVQKTPDLSPTLSSKERE
                     GFCSSLPLRGGQGGVSPKDIKRAIEDLAGRGLLTQHDNGSIAATSFGKKVAQTGIRVA
                     TGKAIEQACSSKTRFDALDWFYFLCGTGEAANLPVTLNFHERTSDYYHGLWLKEKTNF
                     PPDSGPLVSGQMVVRLKLALLLSDWIDGVVTFDLEKKFFSPAGTIQQVAETLEWLLDA
                     AAQVALVLCPGQKLSPELKSLSRRVKWGVREELLPFFDALGPAAGRDHLLKLVAAGWK
                     TVEGLKQATVGELRGVVPQKIAERIVAHLQWPQDGTPAKGKS"
BASE COUNT          393 a          603 c          588 g          414 t
ORIGIN      
        1 ctcaaagtcg tcgtctccac caaagaccgc cgggcgttcg acggccccat tgagcgcggg
       61 cagttcgacc tcgccatcct catctacgaa aaattcaacc agttattgat ccgcaacctt
      121 gatctcctct ccctcatcga ccttctggtg atcgatgaga ttcaaatgct ggacgacccc
      181 acgcgcggcc ccgtcttgga attagcgctt ttgaaagttc tgtcctttgc gaaaagaccc
      241 cagatcgtcg ccctctccgc cgttctggaa gatgccgcgc ctttagccga ctggctcggc
      301 agccgtctgc ttgtggagcg caagcgcccg gtggaactct ttaccggcgt cttgctcgag
      361 agccgctttg ccttccgcgc cttcaatacc aaagaagaag gcgaggaaca gtgggaggtg
      421 gaaagtgccg caggagagga caaaattgac gccgaccagc gggcgcggct ggtgacgcag
      481 attcaaaagt gcgtggcggg gggcgacccg gtcttggtct tcctcaaagg caaaagagat
      541 tgccagggct tagcgcagct tctggccgaa gccgccccct ggccgcctgc ggcgggtgcg
      601 ttagaagaga tcgaagccga gccggaggtg gtgctttccg agtcgctcgc ctattgcttg
      661 cgcagaggcg tcgccttcca ccacgccgac ctcccctttt cgctccgccg gattgtggag
      721 cactcgtttc tggcgggcga aatcaaaatc gtctgcgcca cgacgactct ggctttgggg
      781 gtgaacctgc ccgcccagac cgtgttcgtc gagccctgga agtaccgcaa cggcccgcag
      841 accaatcggc ccgttttggt gcccctctct gtggctgagt tcgagaatat ggcgggtcgg
      901 gccggacgtt tgaacttggc cccttccggc cgcgccgtgc ttctggccaa aacgcctctg
      961 gagcaggaag tgctgtggag taactacgtc accggccggc gcgagaaaat cgcatcccgc
     1021 ctgtcgcacc tgcctctggg cgacatcgtc ctcgatttgg tcgccgcaga gagcgtcaaa
     1081 acggtcgagg aactggaagc aacgttgctg aagtcgttcc actgggttca gaaaacccct
     1141 gacctctccc cgaccctctc ctcaaaggag agggagggtt tttgttcttc cctccccttg
     1201 aggggagggc agggaggggt cagcccaaaa gatattaagc gcgcgattga ggaccttgct
     1261 gggcgtggtc ttttgaccca gcacgacaac ggcagcattg cggccacctc ttttggcaaa
     1321 aaagtggccc agaccggcat tcgggtggcc accggcaaag cgatagagca ggcctgttcg
     1381 tccaaaactc gatttgacgc tttggactgg ttttattttc tctgcggaac cggggaagcg
     1441 gcaaaccttc ccgtcactct gaactttcac gagcgcacct ccgactacta ccacggtctc
     1501 tggctcaaag aaaaaacgaa tttccctccc gattccgggc ctctggtctc cggccagatg
     1561 gtcgtccggc tgaaactggc acttttgctt tcggactgga tcgacggggt tgtcaccttc
     1621 gatctggaaa agaaattctt ttctcccgcc ggcacgattc agcaggtggc cgaaacgctg
     1681 gaatggctcc tcgacgccgc cgcgcaggtg gcgctggtgc tctgtcccgg tcagaaattg
     1741 tccccggaac tgaaatccct ctcacgccgg gtcaaatggg gagtgcggga agaattgctg
     1801 ccgttttttg atgctctcgg cccggctgcc ggccgcgacc acctgctgaa actggtcgct
     1861 gctggctgga aaacggttga gggcctaaaa caagccacgg tcggggaatt gaggggtgtc
     1921 gttccgcaaa aaatcgcgga gcgaattgtg gcccatctcc aatggccgca ggatggcaca
     1981 cccgcaaaag gaaagtct
//
LOCUS       DBNGZA010000231         3884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2905100, whole genome shotgun sequence.
ACCESSION   DBNGZA010000231 DBNGZA010000000
VERSION     DBNGZA010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2905100"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="AC16FU_04815"
     CDS             <1..1401
                     /locus_tag="AC16FU_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2993728.1"
                     /translation="RGQLVFDAADLPVSSNYLSQLSHSGITADQVSRWLNAASVSLTY
                     PQALAAANLPFVREIRPVMRSVQLLPLIEPEVQIPYAPAAPEALNYGNSFRQVHQIKA
                     DSLHALGYTGQGILIGMLDTGFFTDHAAFDSLRLQGRLIATRDFINGDFDVQDNAFDG
                     QRNHGTSTFSAVGGFVPGTLVGTAFGASFALAKTEILATEYVISEEDRWVAGLEWLDS
                     LGADVVSSSLGYNVGDTGFSYTPAQMDGNTAITTQAADLAASRGLLVVNAAGNEGDGP
                     WRIIIAPADGDSVLAVGAVDSLGSRVFFSSTGPTADGRIKPDVMAQGRADWLASAGGG
                     YGRGSGTSFATPLVAGVVALLLDVDSALTPDQLIQRLHQSGSRASNPDTLMGWGIVNA
                     FAASNLGCARRGDVDNSSVVDIADITALVQDIVFGTPLPNPVAGDANCDGVRDVIDIV
                     LLIQFVVFGSPTPCCL"
     gene            1715..2908
                     /locus_tag="AC16FU_04820"
     CDS             1715..2908
                     /locus_tag="AC16FU_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993729.1"
                     /translation="MPGLWNNDALTQSLAAIQNGQMVPQFARVAEELQRQKKIDEAVA
                     ICETHLLQFPNYATGHLVLARLYHEKNRLEEARQEYGQVLKLDPENLAAFKGLGDVFL
                     AFGEKGLALWHYRRAAEIDPLSAEVGILVAELSRDEGAWLPAPPTLPGGAARDSRPVQ
                     SQAGSQELPEVDLEVEYTPPPLPKAPEILKAEFASQLGQVKTKEVDADQVLIGFEETG
                     TAPVESNRAELRPGGAPTHTESLAAHETQSLEKPVARPGGAAPVPPPVTPGVGAPLRG
                     ETEAEPTATARAETPPPMDEAEAAIDRAFAGFEEPTGETSHPKTGKVETARTRPAPAR
                     PAGGLAHPLFETESIADIYARQGLGEQALKIYRKLLTQKPDNPVLQEKIKRVESQLSA
                     KTPVK"
     gene            2994..>3884
                     /locus_tag="AC16FU_04825"
     CDS             2994..>3884
                     /locus_tag="AC16FU_04825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR2993730.1"
                     /translation="MKVLAAVARERLDCLVVTSGANVRYLSGFTGSNGLVVLTPRRTY
                     FFSDHRYDLQAHQEVPGAKIVIGTGDLFALLPQIPFLRTPKVKMGCESGHVTVRGWEL
                     LKKHLPQALPLPTENLVERVAAIKDAGEIAAIEQAAGIADRCFEKIVNFLRPGLAERE
                     VAAELEYQMKQDGAEGAAFETIVASGPRSALPHGRASDRKIQKGDFVTLDFGAVVNGY
                     ASDLTRTVVMGRASSRQKKIYRIVLEAQKRVINAARAGIVAKDLDARARGYIAHWRLG
                     KYFQHGLGHGLGLEVHQLPGI"
BASE COUNT          772 a         1174 c         1194 g          744 t
ORIGIN      
        1 cgcggtcaat tggtattcga tgccgccgat ctgccggtat cctctaatta cctctcgcag
       61 ctttcccatt ccggaataac cgcggatcag gtctcgcgct ggctcaacgc ggcgagcgtt
      121 tcgctcacct atccgcaggc gctggcggcg gcaaatctcc cttttgtccg ggaaatccgg
      181 ccggtaatgc gctcggtgca gctgttgcct ttgattgaac cggaggtgca gatcccctac
      241 gctcccgcag cgccggaagc gctgaactac ggcaactcct tccggcaggt acatcagatc
      301 aaggccgatt cgctccacgc gctgggctac acggggcagg ggattctcat cggcatgctg
      361 gataccggct tctttaccga ccatgcggcg ttcgacagtc tccggctgca ggggcggctt
      421 atcgccacgc gcgatttcat caacggcgac ttcgacgtgc aggacaacgc cttcgacggc
      481 cagcgcaacc acggcacctc caccttctcg gcggtcggcg ggtttgtccc cgggacgctg
      541 gtcggcaccg cctttggcgc gtcattcgcc ctggcgaaga ccgaaattct ggcgaccgaa
      601 tacgtcatct ccgaggaaga ccgctgggtg gcggggttag agtggctgga cagtttggga
      661 gcggacgtcg tttccagttc tttgggctac aacgtgggcg acaccggatt cagttacacg
      721 ccagcgcaga tggacggcaa caccgccatc accacgcagg cagcggatct cgccgcttcc
      781 cgcgggcttt tggtcgtcaa cgccgccggc aacgagggtg acggtccctg gcgcatcatc
      841 atcgcgccgg cggatgggga cagcgtactc gcagtcggcg cggtcgattc gctcggttct
      901 cgcgtctttt tcagttccac cggccccact gctgacggac ggatcaagcc cgacgtgatg
      961 gcgcagggga gggccgattg gctggccagt gcggggggcg ggtacgggcg cggcagcggc
     1021 acttccttcg ccactcctct ggtggccggc gtcgtggcgt tgcttctgga cgttgattcc
     1081 gctcttaccc ccgaccaact tattcaacgt ctgcaccaat ccggcagccg ggcttccaat
     1141 cccgatacgc tcatggggtg ggggatcgtc aacgccttcg cagcatccaa tctgggctgc
     1201 gcgcggcgcg gggacgtgga caacagcagt gtcgtcgaca ttgccgacat cacggcgctg
     1261 gtgcaggaca tagtgtttgg cacgcccctg ccgaatcctg tagcggggga cgcgaactgc
     1321 gacggagtgc gggacgtcat agatattgtg cttttgattc agtttgtggt ttttggcagc
     1381 cccactccat gctgcttgta atttggggcg tgtggtgagc ggagccgaac cgcagcccga
     1441 caccgtgctg tctctaaaac cagggaatag ttgaggggac gagacctcac agcatttggg
     1501 gtgacccccg ctcaccccgc cccgggcggg atgttccaca aatcccggtt tttgccgata
     1561 ttttaacgta gtaagccgag agggtagacg accacagggt cgtggtacgt ctaacccttt
     1621 tagcacgttt tgcttaaagt gatgagagaa atttcgcttg acagtccgcc tcaggcggat
     1681 aggtttcagt cgaacgtaga ggagatttta gcgcgtgccc ggattgtgga ataacgatgc
     1741 cttgacccag tcactggcgg ccatacagaa cggccagatg gtgccgcaat ttgcacgcgt
     1801 ggccgaggag ttgcagcggc agaaaaaaat cgacgaggcg gtcgccatct gtgaaacaca
     1861 tctgttgcaa ttcccgaatt acgccacggg gcatctggtc ttagcgcggc tgtaccacga
     1921 gaagaatcgc ctcgaagaag cgcggcagga gtacggccag gtgctcaaac ttgacccgga
     1981 aaatctggcg gcgttcaagg gtttgggaga cgtcttcctg gccttcggtg agaaaggact
     2041 ggcgctctgg cattaccggc gggcggcgga aattgaccct ttgagtgccg aagtggggat
     2101 cctggtggct gaactttccc gggacgaagg cgcatggctt ccggcccctc caacccttcc
     2161 cggcggtgcc gcacgagaca gccgcccggt tcagagccaa gccggttctc aagagttgcc
     2221 ggaagtggat ctggaagttg aatacacgcc gccgccgctc cccaaagcgc cggaaattct
     2281 caaggccgaa ttcgcttccc agctggggca ggtgaaaacc aaagaggtcg atgccgatca
     2341 agtcttaatc ggttttgaag agaccggcac ggctcccgtc gaatcgaacc gcgcggagct
     2401 ccggcccggc ggcgcaccaa cgcacacgga gagtctggcc gcccacgaaa cccaatctct
     2461 tgagaagccc gtggccaggc cgggtggagc agcgcctgtt cctccgcccg tcacacccgg
     2521 cgtcggtgcg ccgcttcgag gtgagactga agccgaaccg accgcaaccg cccgagcgga
     2581 aactcctccg ccgatggacg aagcggaagc cgccatcgat cgggcgttcg ctggttttga
     2641 ggaaccaacg ggtgaaacca gtcaccccaa gaccgggaag gtggaaaccg cgcggacccg
     2701 accggcaccg gccaggcccg ccggcggttt ggcgcacccc ctcttcgaaa ccgaaagtat
     2761 agccgacatc tatgcccgac agggtttagg ggaacaagcc ctgaagattt accggaaact
     2821 cctgacgcag aagcccgaca atccggtttt acaggaaaag atcaaacgcg ttgaaagcca
     2881 actctccgcc aagactccgg tcaagtagtg tcggggtgtc gaggtatcga ggtgtcgagg
     2941 ttaaccccga cactccgaca ctccgacacc tcgacaccct cttacggcgg cgcatgaaag
     3001 tgctggcggc agtcgccagg gaacggctgg actgtcttgt cgtcacctcc ggcgccaacg
     3061 tccgctatct ctccggcttt accggatcaa atggcttagt cgttttgact ccccgccgga
     3121 cctatttctt ttccgaccac cgctacgatc tgcaggcaca ccaggaagta cctggcgcta
     3181 aaattgtgat cggcaccggc gatttgtttg cgctcctccc gcaaattcct ttcctccgca
     3241 cgcccaaggt gaaaatgggc tgcgaatcgg gccacgttac cgtacggggc tgggaactac
     3301 tgaagaaaca cctgccgcag gccctccctc tccccacgga aaatttagtc gagcgggtcg
     3361 ccgcgataaa agacgccggc gaaatcgccg caatagagca agccgctggc atcgccgatc
     3421 gttgctttga aaaaatcgtg aacttcctgc ggccggggct tgcggagcgg gaggtggcgg
     3481 cggaattgga gtatcaaatg aaacaggacg gggccgaagg agcggcgttt gaaaccatcg
     3541 tcgcctccgg cccgcggtcg gctctgcccc acggccgggc ttcggaccgg aaaattcaaa
     3601 aaggcgattt cgtcacgctg gacttcggcg cagtcgtgaa cggctacgcc tccgacctca
     3661 cccgaaccgt tgtcatgggc cgggcctcct cccggcagaa aaaaatttac cgcatcgttt
     3721 tggaagcgca aaaaagagta atcaatgcgg ctcgtgcagg tatcgttgcc aaagacctgg
     3781 atgcccgggc ccgggggtat attgcacatt ggcggttggg gaagtatttt cagcacgggt
     3841 taggtcacgg cctggggttg gaggtgcacc agctgccggg gatc
//
LOCUS       DBNGZA010000232         1196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2057337, whole genome shotgun sequence.
ACCESSION   DBNGZA010000232 DBNGZA010000000
VERSION     DBNGZA010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2057337"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16FU_04830"
     CDS             complement(<1..533)
                     /locus_tag="AC16FU_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993731.1"
                     /translation="MLRVARLRLLLLAGIFVLAPLVASAAKKEKKEKKQEPLVLENAD
                     AYLRVDSAGMVIHHLDGHVLFKHGDARLQSDHADWYETQKRIEFSGRVVFNYPDQQVK
                     SEHVTYYIPDKRALGTGGVELIDLKEDAVVTGGRGDYNRNEKHLRVTENPVLVLKAKD
                     TLQTVTVTSHFMEYFAE"
     gene            complement(626..>1196)
                     /locus_tag="AC16FU_04835"
     CDS             complement(626..>1196)
                     /locus_tag="AC16FU_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2993732.1"
                     /translation="LVVIFVPFIAYFRLKERMTLNLVAGALLALLGLYLLNRPHGWTV
                     RWGDLATILCAWFFAWQLVYINIYTPRFAIPHLITVQFGVSTVAGLVLTVLFEKPPLA
                     YPPKLWGSVLLMGLLATALAFYLQNRFQKDTTTVRAAIIYNLEPIFAVLFAFLLLGEV
                     LEVSEYLGGAAIIGGVLVAEVGKGRSTVF"
BASE COUNT          264 a          338 c          323 g          271 t
ORIGIN      
        1 tcctcggcga agtattccat gaagtgtgac gtcaccgtta cggtctgtaa cgtgtctttg
       61 gccttgagca ccaaaaccgg gttttcggtg acgcgcagat gcttttcgtt tcggttgtag
      121 tctccccggc ctcctgtgac cacggcgtct tccttgagat caatcaactc caccccgcca
      181 gtgccaaggg cccgcttgtc cgggatatag taggtgacgt gctctgattt cacctgttga
      241 tccgggtagt tgaaaaccac ccgaccggag aattcaattc ttttctgggt ttcgtaccag
      301 tcggcgtgat cgctctgcag ccgggcgtcg ccgtgcttga agaggacgtg gccgtccagg
      361 tggtggatca ccattccggc cgaatcgacc cgcaggtagg catcggcgtt ttccagcacc
      421 agtggttcct gcttcttttc ctttttttct tttttcgctg cgctggcgac taacggagcc
      481 aggacgaaga tcccagccag caacagcaag cgtaatcggg ctactcttag cataatcggc
      541 cgaaaggcgg tttgttttct gctctcttcc actatcaggt ttctttacgg aaatggagtg
      601 cgtttgatga attcgttttc atccgttaga aaactgttga ccgccctttg cccacctccg
      661 ctaccagcac tccaccaatg atcgccgctc cccccaggta ttcggacact tccagcactt
      721 ccccaagcag aaggaaagcg aacagcaccg caaaaatcgg ctctaagtta tagataatgg
      781 ctgcccgcac cgtagtcgta tctttttgaa agcgattctg gaggtagaac gccagggccg
      841 tggccaaaag ccccatgagg agaacgctcc cccacagttt gggaggatac gccagcggcg
      901 gcttttcaaa gagaactgtc aagaccagtc cggcaaccgt gctcacccca aattgtacgg
      961 taatcaggtg gggaatggcg aaacgcgggg tgtaaatgtt gatatagacc agctgccagg
     1021 cgaaaaacca ggcgcagagg atggtggcca gatcccccca gcggacggtc cagccgtggg
     1081 gccggttcaa aaggtagagt cccaaaagcg ccaggagcgc cccggccacg agattgagcg
     1141 tcatccgttc tttcagtcgg aaataggcga tgaacggcac gaagatcacg accaga
//
LOCUS       DBNGZA010000233         3910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1844878, whole genome shotgun sequence.
ACCESSION   DBNGZA010000233 DBNGZA010000000
VERSION     DBNGZA010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1844878"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="AC16FU_04840"
     CDS             <1..211
                     /locus_tag="AC16FU_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993733.1"
                     /translation="AGSLEMFLTRATSHILQSSGLSCISGVMVKWRTSGRKNCAWQIC
                     AKELMTSIASNLVLIVALSISELMF"
     gene            complement(194..844)
                     /locus_tag="AC16FU_04845"
     CDS             complement(194..844)
                     /locus_tag="AC16FU_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993734.1"
                     /translation="MKTEYQNWLDADLLKDSTVAKAIRDAFKQAKTDISFFQSFVRID
                     TLTFVDDVLFYQAYRDSFKTDKTNATYPYYLVSAQKLNVNAGGGFGIKAGVSFVPGPN
                     SPPFGNQRWSFVFYKDILANDALLPGEFWRYLAQVTIHELGHQRSGLTHANLDEVNCS
                     VAVVNHDDGGSGAKCVMNFGACITDAPFGVLQTLSFCNNKDTTKTNNCVYKLKHQF"
     gene            1283..3910
                     /locus_tag="AC16FU_04850"
                     /pseudo
     CDS             1283..3910
                     /locus_tag="AC16FU_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          837 a         1047 c         1100 g          926 t
ORIGIN      
        1 ggcaggttct cttgaaatgt ttttgacccg tgctacaagc cacattctgc agtcttcagg
       61 attatcttgt atttcaggtg tgatggttaa atggaggaca tctggccgga agaactgcgc
      121 ttggcagatc tgcgctaagg aactcatgac aagtatcgca agcaatctcg ttctcattgt
      181 tgcgctcagc atttcagaat tgatgtttta atttgtaaac gcagttgttt gttttggttg
      241 tatccttatt gttacaaaaa ctaagagttt gcagaactcc gaatggggca tccgttatgc
      301 acgctccaaa attcatcacg cactttgccc cactgccgcc gtcatcatga tttacaacag
      361 caacgctaca atttacctca tccaaattag cgtgagtaag tccagacctt tgatgcccga
      421 gctcgtgaat ggtaacctgt gctaggtagc gccagaattc accgggcaaa agggcatcgt
      481 tagctaaaat atctttgtaa aaaacgaaac tccagcgttg gttcccaaag ggtgggctat
      541 tagggcccgg tacgaatgaa acgccggctt taatgccaaa acctccacct gcgttcacat
      601 tgagcttctg ggcagagaca agataatagg gataagttgc attggtttta tcggtcttaa
      661 aagagtcacg ataggcttga tagaataata cgtcatccac aaacgttaag gtgtcaattc
      721 gcacaaaaga ttggaaaaaa gaaatgtcgg tcttggcttg cttaaacgcg tcccgaatag
      781 cttttgcgac agtgctgtct ttaaggagat ctgcgtcaag ccaattctgg tattcggtct
      841 tcatagaaag agatgcaaca ttcggagggg gaatgatatt cgggatacgt atcgtacaat
      901 gtaccgtaag aaataggcag ccagtcgtta tcattaggca ccaaagaaac ttacgcttcc
      961 ccatgactgt aatctaacaa aaaaaaaaaa acggttttgt caatgatttt ttcatttttt
     1021 agacaatttt ctcttatttt tgttgaagtc attgcgggtc ttggacttga ctgctggtca
     1081 cagatgaagt cctggttttt acgtggaacg gtgccgtgtc ccccgggtat gtacaaacct
     1141 gcgccggact tgtatattgc ggtctttcga ttctggattc gctggtccta agaagatgga
     1201 gtaacctatg aaacagaaag attacatcgt tgctactctc acggcgggat ttatttactt
     1261 tcaggcacag gcgggcgggg taatgagcgc aacggttgat ccggctctcc aagtccgact
     1321 ggcacaggca cacagcagcg atacgctgcg cgtccttttg gtcttggctg acagagtgcc
     1381 gacggcgaaa cttaactccg atctcaaaac tttgactcaa agccggaccg tgcggcacaa
     1441 gcaggtggtt gaagcgttgc aagccaaaac ccgggcgacc caggcagatc tcctgggcga
     1501 attagagcgg gaaagagagc ggggtacggt cgctgcctac aaagggtact gggtcgacaa
     1561 cattatctgg gtgcggggag agaaaggggt cgtcgaaagg tatttgaatg atccgcgggt
     1621 ggcggaggta tacggcgagc cgcaggtgca attggtggag ccggtcgggc ccgggtcaca
     1681 ggctccctta gttcccggcg ggactgagcc ggggctgcgg gccatcaagg ctgactccgt
     1741 ctggctgatg ggttacactg gccgcggacg gttagtgtgc aatttcgata atggagtatt
     1801 agggacgcat cacgctttta gcacccgctg gcgggggaat aatggtgcac ctgcttctgc
     1861 ttgctggttc gaccctacgc gcaacagcac ctccccggac acctttcaaa tcagcaccaa
     1921 ctggcacggc acctttacga tgggaattat gtgcggccgc gatccgttga ccgccgacac
     1981 catcggtgtt gccttcggcg cgcagtggat cgccgccgga gtgatcgaca tcagcagtgc
     2041 gaccagttaa ctggttttgg acgcctttga atgggtcatt gaccccgacg gtgatccgaa
     2101 cacgatagaa gacgtgcccg acgtgttgaa caattcctgg ggcttgcccc gctttgacgg
     2161 cacctgtcag ccgctggata tcggctgtga tcaggtcttc tggggagcga tcgacaacct
     2221 tgaggcggcg gggtgcgtgg tgctctttgc ggcggggaac gaaggggcct gcggagcggc
     2281 gtcgctccgg aatccggcga accgcgccac gaccgccaac aacgtttttt ccgtcggagc
     2341 caccgccaca acctcgcctt tcaccatcgc ctctttttcc agccgcggcc cctcggactg
     2401 tgacggggtc tccatcaaac ccgaagtggt ggcgcccggc ctcaatgtcc gctcctccgg
     2461 gtggccgacc ggagcccgca ccaatgcctc ctatggcact tcctccggaa cctctttctc
     2521 cactccctac gtggccgggg cggtggcgct gttgcgggag tacaatccga acctcacggt
     2581 agatacgatc aagtgggcgc ttttgaattc ggcgctagat cggggaacgc cgggcgagga
     2641 caatacctac gggcgcggat tcatcaacgt caaggccgcg ctgggtcttt tgccgccgaa
     2701 tcagggcgtg cacctctttg tcaaacgcga catcctgacc gatttgaacg gcgggacggt
     2761 ggaaccgggg gacacggttg atctcctgct acggctgggc aactcggggg cggacacctc
     2821 cttcggcacc tggggccggc ttttttcgac cgattcccgc atttcagtcg ttcctggttt
     2881 tgactctgcc tttttcggta ccgtggcgcc gaaagacagc gcggccaatt cgggaacgct
     2941 tctgcgcttc gcttttgacc cgacggcacc ggcaaatata aatcttccct tgcaactaac
     3001 cgttttctcg aacgccgggc cagaggatac cctgcccttg ttcttccata ccggggaccg
     3061 gctggtgcgc tcaattgtga accactcact ggctaacaac ttctttaccg tttccaattt
     3121 tgggcagtac ggctttgcta ccggttcact tatgccacgg agccctggcc aggggttttt
     3181 ctatccaaaa gcaggtggag cgaataacct ctttgagatg gctttcctgg tggggacgag
     3241 cgacagcacc gtctcgaacg ctgtgcgcgg ccagccctcc gatctcattg accgcgactt
     3301 tgtgccgatt acgggcggga atctgatccg ccaggctccg ggtcctttag ccaatgaaga
     3361 gacgttctgc cgcttcaatg acagcggggc gatccggcca ttggggttgg aggtcaacca
     3421 gcggaccttt gctttttcag atacggcgaa tgacgactac gtgattttgc agtatgccgt
     3481 caagaatgtt tccggccaga cgcggaccgg agtccggatc ggtctctttg ccgactggga
     3541 tttcccctac ggttcgggcg ccagcgattc ggtcaatttc gatgccggcc gcaacctggg
     3601 ctacatgttc gacgggggaa acccttcggc ccgcaagccc cgcggggtgg cggtcctcac
     3661 tctcccggga gcgaccgcct accgggccat taacagcccg accgagattt acgacggatt
     3721 ttctcaggca gaaaagtggc agggaatgag tcagggtctg attacgctcc gctcgggagg
     3781 gggcgaccgc tcccacctga ttaccaccgg tcctttcgat cttttccccg gtgattcggt
     3841 caaagtggct ttcgccgtcg tgggcggtgc gaccttcgcc cagttgcaag ccaatgccga
     3901 ttcggccaag
//
LOCUS       DBNGZA010000234         4768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_44386, whole genome shotgun sequence.
ACCESSION   DBNGZA010000234 DBNGZA010000000
VERSION     DBNGZA010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_44386"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="AC16FU_04855"
     CDS             complement(<1..1230)
                     /locus_tag="AC16FU_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR2993735.1"
                     /translation="MFVATRYILREQIGPFVFAFAVITFVLVMDFILEVINLVVAKGL
                     SVWTVLELFALNLAWMLALSVPMAVLVATLMAFGRLGADHEVTAFKACGVNPVRLLLP
                     VLAAATVLAGLMVFFNNQVLPEANHRARLLLAAISQKRPTLRIEPHVFIDDVPGYHFI
                     VKKVDPRSGKISGVTIYDQKERPYPRTIIAKRGEIAFAADGTTLVFTLYDGEVHEADE
                     NDPTKYRRVAFDKQTVYMREAGSQLIRQESDYRTDREMSAAEMQAKNRQTRANLDGLQ
                     KQLRTAAAQRVSQLLTGNPLSGGTPSAPPDSAVTARAAVEEQAFALRLQEPASLVTSF
                     QQEIKSLAVEIHKKYAIPVACVVFALIGAPLGILVRRGGFGTSAGISLGFFIFYWACL
                     IGGEELADRRFISAFWAM"
     gene            1372..2337
                     /locus_tag="AC16FU_04860"
     CDS             1372..2337
                     /locus_tag="AC16FU_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR2993736.1"
                     /translation="MLSDLKVGDTFTGFAVLRKKELREYDGGKFLRLELGDRSGRIDG
                     VVWENAALLFPNLFVGDVVKIKGYVSLYRDTPQIKVDTIALAHQGEYERKALVPASEK
                     TTEQLIAEFKTVVTTVTQPQLRKLLDLFATDEPLLRAYAAAPAAKLWHHAYEGGLLEH
                     TLQVVQICDFAARLYAVVERDLLIAGALLHDIGKVRSYQLSTLIDFTDEGRLVGHINT
                     GDQIVCEKIKQIPDFPENLALRLRHLIIAHQGELQLGSPVVPQTAEAFVLHYADELDA
                     KLGALTRIITRERGQGKTWSDYVRLIDRYIYLGESASGGTQTGSE"
     gene            2349..3377
                     /locus_tag="AC16FU_04865"
     CDS             2349..3377
                     /locus_tag="AC16FU_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR2993737.1"
                     /translation="MSFFDLISNDIGIDLGTANTLVFVRGEGIVLNEPSVVAVEVATK
                     KVLAIGAAAKEMLGRTPGEIVAIRPLKDGVIADFEITERLLADFIKRVVRHRYLMKPR
                     IVISVPSGITEVEKRAVRDSAENAGAREVYLIQEPMSAAIGVGLPVDKPAGNMVIDIG
                     GGTSEIAVIALNGIVNNTSIRIAGDEMNEAIVTYLKKNYNLLIGELTAEEVKIKIGSS
                     FPLDKEETMEIRGRDLVAGVPKTMKISSVQVREALAETVDAIVEAVRQALERTPPELA
                     ADILDRGIILTGGGALLRGLDKRLRQETNLPVNVADDPLTCVVRGTGKVLENMNHYSK
                     VLIKSRRD"
     gene            3503..>4768
                     /locus_tag="AC16FU_04870"
     CDS             3503..>4768
                     /locus_tag="AC16FU_04870"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR2993738.1"
                     /translation="MTDQRQPPRTIPELFAASVRAYGQLPALLAFRQRRWQSFSYRQI
                     KEQADQVASGLLTLGLKRGDRVGILSENRPEWGIAYLAIAAIGCVVVPLDMLLKSSEH
                     EEILRDAEIRAVFASGQFLPQLKALRPLVPTLRFLISLEETADRAVVSWSQMTQRTKG
                     QLAFIGPKPDDLLALIYTSGTMSTAKGVMLSQKNITANVAGCAEVVIFGPQDRFLSVL
                     PLSHTYECTAGFLVPLAKGAAVAYARSLKSKEILEDIGATGATLMLGVPLLFEKNLAG
                     IWRGIGKQPFGKRTVVQILYRLVKVVKRSTGINLGIAVFSGLRRRAGLGALRMFVCGG
                     APLDPATAEGFDLLGIKFLQGYGLTETAPVLTVNPDYKQKYASVGKPIPGVELAIINP
                     DGRGVGEIAARGDSVMLGYYKNPKATAQVL"
BASE COUNT          986 a         1392 c         1493 g          897 t
ORIGIN      
        1 catggcccag aaggcagaga tgaagcggcg gtcggctagc tcttcccccc cgatcaagca
       61 ggcccagtaa aaaatgaaga aaccgaggct gatcccggcc gacgtgccga accccccccg
      121 tcgcaccaga ataccgaggg gggcgccgat gagcgcaaaa acgacgcagg cgacggggat
      181 ggcgtacttt ttgtggattt ccaccgccag ggacttgatc tcctgctgga aactggtcac
      241 taatgaggcc ggctcctgga gccggagcgc gaacgcctgc tcctcgactg cggctcgggc
      301 ggttactgcg gaatcaggcg gggcgctggg ggtcccaccc gagaggggat tccccgtcaa
      361 gagttgcgac acgcgttgcg ccgccgcggt ccgcagttgt ttctggagcc catccaggtt
      421 cgctcttgtc tggcggttct tggcctgcat ttcggcggcc gacatttccc ggtcggtgcg
      481 gtagtcagat tcctgccgga tcaattgcga cccggcctcc cgcatgtaaa ctgtctgctt
      541 gtcaaaagcc acgcgccggt acttggtagg atcattttcg tccgcttcgt gaacctcgcc
      601 gtcgtagagc gtaaaaacca acgtcgtccc gtcggcggca aaggcgatct ccccccgctt
      661 ggcgatgatc gtgcgcgggt aggggcgctc cttctggtcg tagatggtga cgccggaaat
      721 ctttccggag cgcggatcga ccttcttgac gatgaagtgg tagccgggga cgtcatcgat
      781 gaagacgtgg ggctcgatcc gcaaggtcgg gcgcttctgg ctgatggccg ccagaaggag
      841 ccgcgcccgg tgattagctt ccggcagcac ctgattattg aagaaaacca tcaagcccgc
      901 caggaccgtt gcggcggcca gaaccggcaa aagcagccgc acggggttca ccccgcaggc
      961 tttgaaggcg gtcacctcgt ggtccgcccc cagacggcca aaggccatca gggttgctac
     1021 cagcaccgcc atcggcaccg aaagggcaag catccaggct aagttcaggg cgaacagttc
     1081 gaggaccgtc cagaccgaaa ggcccttggc cacgacgaga ttgatcactt cgagaatgaa
     1141 gtccatcacc agaacgaaag tgatcacggc aaaggcgaag acaaaagggc cgatctgctc
     1201 gcgcaggatg tagcgggtgg cgacgaacat cgcgcggcaa tatacgggag agcggtgccg
     1261 caacaatcgc ttttccttga caaccgaatg gcagcggctt tatatgcaga cattcccccc
     1321 cgcgtgtggc gggggatttc cgaaagggag aagcgataag tcagggatgc aatgctgagc
     1381 gacctcaagg taggggatac tttcaccggc tttgcggttc tccggaaaaa ggaactgcgg
     1441 gagtacgacg gcggaaaatt cctgcggctg gagctgggcg accgctccgg ccggatcgac
     1501 ggcgtggtct gggaaaatgc cgccctgctc tttcccaatc tcttcgtggg tgacgtggtg
     1561 aaaatcaaag gttacgtctc cctgtaccgc gacaccccgc aaatcaaagt cgacaccatt
     1621 gcgctggcgc accaggggga gtacgagcgg aaggcgctcg tcccggcctc ggagaaaacc
     1681 actgagcagt taatcgccga gttcaaaacc gtggtgacga cggtcaccca gccccagctc
     1741 cgaaaactgc tcgatctctt tgccaccgat gagcccctgc tgcgggcgta tgccgccgcg
     1801 cccgcggcca aactctggca ccacgcctac gaggggggac tgttggagca caccctgcag
     1861 gtggtgcaga tctgcgattt cgccgcccgg ctgtacgcgg tcgtcgagcg cgaccttttg
     1921 attgccggcg cactcctgca cgacatcggc aaggtgaggt cctaccagct ttcgacgttg
     1981 atcgacttta ccgacgaggg acgcttggtg gggcacatca acaccggcga ccagatcgtt
     2041 tgcgaaaaga tcaaacaaat tccggatttt ccggaaaacc tggccctgcg gttgaggcac
     2101 ttgatcattg cccaccaggg agaactgcaa ttggggtcgc ccgtggtgcc gcagacggcc
     2161 gaggccttcg tgctccacta cgccgacgaa ttagatgcca agctgggggc cctcacccgc
     2221 atcatcaccc gggagcgggg gcagggcaaa acctggagcg attatgtgcg cttaatcgac
     2281 cgatatatat acttaggtga atccgcctca ggcggaacgc aaaccggaag cgagtgaatg
     2341 gaggcaggat gtcttttttt gatttgatct ccaatgacat tgggatcgat ctggggaccg
     2401 ccaacacctt ggtcttcgtg cggggagagg ggatcgtact caacgagccc tcggtggtgg
     2461 cggtggaagt ggcgaccaaa aaggttctgg cgatcggcgc ggcggccaaa gagatgctgg
     2521 gccgcacgcc gggagaaatc gtcgccatcc ggcccctcaa ggacggggtc atcgccgact
     2581 tcgagatcac cgagagactt ttagccgatt tcatcaaacg ggtggtgcgg caccgctacc
     2641 tgatgaagcc gcggatcgtg atctcggttc cttccggcat caccgaagtg gagaagcggg
     2701 cggtgcgcga ctcggcggag aacgctggcg cccgggaggt ctacttgata caggagccga
     2761 tgtcggccgc catcggcgtc ggcttgccgg tggacaagcc ggccgggaac atggtgatcg
     2821 acatcggcgg cggcacttcc gagatcgccg tgatcgcctt gaacggcatc gtcaacaaca
     2881 cctcgatccg gattgccggc gacgaaatga acgaagcgat cgtcacctac ctcaagaaga
     2941 attacaatct tttgatcggg gagctgactg ctgaagaagt caaaattaaa atcggctcct
     3001 cgttcccctt ggacaaagag gaaactatgg agattcgcgg ccgcgacctg gtggctggcg
     3061 tgccgaaaac gatgaaaatc tcctcggtcc aggtgcggga agcgctcgcg gaaaccgtcg
     3121 atgccatcgt cgaagcagtc cggcaggcgc tggaacggac accgcccgag ctggcggcgg
     3181 acattctcga tcgcggcatt atcctcacgg gcggcggggc gctcctgcgc ggcctggata
     3241 agcgtctccg tcaggaaacc aacctgccgg tgaacgtggc ggacgatccc ttgacctgcg
     3301 tggtgcgcgg caccggtaag gttttggaga atatgaacca ctactcgaag gtccttatta
     3361 agagccggcg cgactaaaga gaggcggtat caggacgata cgacccaccc cgcctcccgc
     3421 ggggtttttt gtcccttgtg gctatgtccg gcgtccttca tattattatt cgtgtcctta
     3481 cctccacaca caaaggaagg gtatgacgga tcagaggcaa ccgccgcgta ccattcctga
     3541 gctattcgca gccagcgtcc gggcctacgg acaactgccg gccctgctcg catttcgcca
     3601 gaggcggtgg cagagttttt cgtaccgcca gatcaaagag caggccgatc aggtggcgag
     3661 cggactcttg accttggggc tcaagcgcgg agaccgggtc ggcatcttaa gtgaaaatcg
     3721 gccggagtgg gggattgcct acctggccat cgccgccatc ggctgcgtcg tcgtcccgct
     3781 cgacatgctc cttaaatcct cagagcatga ggagattctg cgcgacgccg aaatccgggc
     3841 ggttttcgcc tccggccagt ttttacccca attgaaagct ctgcgcccgc tggtgccaac
     3901 cctgcgtttt ttaatctcgc tggaggagac cgccgaccgc gcggtggttt cctggagcca
     3961 aatgacccag aggaccaaag ggcagctggc cttcatcggc cccaaaccgg atgacctatt
     4021 agcgcttatt tatacctccg gcacgatgtc gacggccaaa ggggtgatgc tctcgcagaa
     4081 aaatatcacg gccaatgttg ccggctgcgc agaagtggtc atcttcggcc cccaagatcg
     4141 gttcctttcg gttttgccac tgtcccacac ctatgaatgt accgccgggt ttctggtgcc
     4201 tctggccaag ggtgctgcgg tggcctacgc gcggagtttg aagtcaaagg agattctgga
     4261 ggacatcggc gccaccggag ccacgctcat gctgggagtg ccgctcctct ttgaaaaaaa
     4321 tctcgccggc atctggcgcg ggatagggaa acaaccgttc gggaagcgca cggttgtgca
     4381 gattttgtac cggctggtca aagtggtcaa acggtcaacc gggatcaatc tcgggatagc
     4441 ggtattcagc ggtttgcgcc ggagagccgg tctgggggcg ctgcggatgt tcgtctgcgg
     4501 cggggccccg ctggatccgg ccaccgccga agggtttgac cttctgggca tcaagttcct
     4561 gcagggatac ggcttgacag aaacggcgcc cgttctgacc gttaatcccg actataagca
     4621 gaagtacgcc tctgtgggca agccgattcc gggggttgaa ctggccatca tcaatcccga
     4681 cggccggggc gtcggggaaa ttgcggcgcg gggcgactcg gtgatgctgg ggtactacaa
     4741 aaaccccaag gccaccgccc aggtgctg
//
LOCUS       DBNGZA010000235         3852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1955093, whole genome shotgun sequence.
ACCESSION   DBNGZA010000235 DBNGZA010000000
VERSION     DBNGZA010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1955093"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2437
                     /locus_tag="AC16FU_04875"
     CDS             <1..2437
                     /locus_tag="AC16FU_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993739.1"
                     /translation="KTPSTLYCGHKVGKLWLMVTNFGQFGDEPDNPYFAENCFPGVNK
                     PGKFSAQYPGGSGTEYLFTGALWCGAVSGKDTLTTTAFDGWLNVSEMFADVAPQGDFT
                     ARSINPGSPFYSPDAISTFDLIAAYTDTMTCSGFVNADQYSGRGHKPLNIKVIQKSYS
                     WDYSYAKDFVLFDYQVINMGKIPLESFWMGIYIDADVWPSRLSSVGFKDDLAGFRQYA
                     LDPATGDSVINVAYIGDNDGDGEGGVFKDKRNTKAVTGTRVVRTPNPDLKFSFNWWIS
                     EGTNPVDQDWGPQTLAKKRQNYSGGWGTPEGDIIKYWYMSNNEFDYDQIQLELEHRDW
                     IPPPKNADSLVDGFDTRYLLSFGPFDVNPGDTLPITLAYVAGDDWHRNANDWKNTFPA
                     GDFSKFKDSAVVNNFINNTLNWDDLARNAQWAGKVYDNPICVDTIINGQPKRICKGDG
                     IPDFKGPPPPTPPALRLDADVGKITVRWNGKETEAKAPDPFSNVLDFEGYRIYLSRSA
                     VAGDFVLLGSYDFIDWQVWYKNEGLSPAVWQRLKTEKRPLTLPEIKQQFGATVDPEVV
                     KDSSVAIRTHRGTGTRDTSFFFRPEDYNRGLDPIRVYRDTAAARRDFPTLSPAALDSL
                     FNDSLKYEYQYSADHLIPSRPIFVGVSAFDFGDVASGLDPLESSPQSNAKFAFPLATI
                     DRVFAAKEKVKVFPNPYKIDAGYGIFEQPQGIPEQATEYDRKIHFINLPPKCTIRIFT
                     VDGDLVREIQHDYSSRPFDPLASYDVWDVISRNTQSVVTGLYLYSIEAQDVPAFPIPP
                     ELKRQVGKLVIMK"
     gene            2467..3510
                     /locus_tag="AC16FU_04880"
     CDS             2467..3510
                     /locus_tag="AC16FU_04880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993740.1"
                     /translation="MMKKAKVLWLLTLAVLGPHLAWGQAKVGTAGAQFLELGVSARAA
                     AMGDAFVSLANDATALYYNPAGLTQLSGKQAVFSGVQLPADMQLGFVGFVLPVAKLGG
                     QMGVSVYGLNSGDIEETDYGHPSGTGRTFGVFDLAVALSYARSLTDHFSMGVTAKFIQ
                     ESFNGVGFRITPSGGQESIDEHSRGWAADVGTLYDTGFRGFRIAMSITNFGPDLKLSS
                     NEFPGEAFKRQAYALPINFKFGTAINVVELPNNRVTLALEGSHPSDNLEKFQGGVEYA
                     WHDILALRAGARARSNVTCEQKSIAAFDRLTFTFGGGVQVPLGQYRLQADYAFQDFGV
                     LDNIHRLSVILSF"
     gene            3566..>3852
                     /locus_tag="AC16FU_04885"
     CDS             3566..>3852
                     /locus_tag="AC16FU_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993741.1"
                     /translation="MNKRLRICLVAAGGGALGLALWLWGCTKGKGVYPDNVPPVIAFV
                     NIPPESTYYSQSPTVYWYATDLDGFVTQYQYTIVTKAKLMADILGIDSAVAA"
BASE COUNT          815 a         1104 c         1131 g          802 t
ORIGIN      
        1 caagacaccc tctacgctct actgtggcca caaggtcggg aaattgtggt tgatggtcac
       61 caactttggg cagttcggag acgaaccgga caacccctat tttgcggaga attgttttcc
      121 cggggtaaat aaaccgggaa agttttcggc gcagtacccc ggcggttcag gcacagaata
      181 cctctttacc ggcgcactct ggtgtggggc agtttctggc aaggatacac tgaccaccac
      241 cgcatttgac ggctggctca atgtgtccga aatgttcgcc gatgtcgccc cccagggaga
      301 cttcactgcc aggtcgatca accccggttc tcctttttac tcgccggatg ccatttccac
      361 tttcgattta atcgctgcct acaccgatac gatgacctgc tccggtttcg tcaatgccga
      421 ccagtacagc gggcgaggcc acaaaccgct caacattaag gtaattcaga agagttactc
      481 ctgggattac tcctacgcca aagattttgt ccttttcgac taccaggtaa tcaatatggg
      541 gaagatcccc ctggagagtt tctggatggg tatttacatt gacgccgatg tctggccctc
      601 ccggctgtcg agtgttggtt tcaaagacga cctggccggc ttccggcaat acgcacttga
      661 cccggccaca ggcgacagcg ttatcaacgt ggcctacatc ggggataatg acggggacgg
      721 cgagggcgga gtcttcaaag acaaacggaa caccaaagcg gtgaccggca cccgggtggt
      781 ccggacgccc aatcccgacc tgaaattttc gttcaactgg tggatttcgg aagggacgaa
      841 tcccgtggac caggactggg ggccccagac tttggccaag aaaaggcaga actactccgg
      901 cggctggggt acgccggaag gtgacatcat caaatactgg tacatgtcga ataacgaatt
      961 cgattacgac cagattcagt tggaactgga gcatcgcgac tggataccgc cgcccaagaa
     1021 tgctgactcg ttagtggacg gatttgacac ccgttacctc ctttcctttg gcccctttga
     1081 tgtcaacccg ggcgacacgc tccccataac gctcgcctac gttgcggggg acgattggca
     1141 ccgcaatgcc aacgactgga agaatacctt ccccgcggga gacttcagca agttcaagga
     1201 ttctgcggtc gtgaataact tcattaacaa tacgctcaac tgggacgact tagcgcggaa
     1261 cgcacagtgg gccggtaaag tgtatgacaa tccgatctgc gtggacacga ttataaatgg
     1321 tcagccgaag cggatctgca aaggcgacgg cattcccgac ttcaagggtc cgccgccgcc
     1381 caccccgccg gcgttgcgtt tggatgccga cgtggggaag atcacggtgc gctggaacgg
     1441 caaggagacg gaggccaaag cgccggatcc gttctcgaac gtgcttgatt ttgaagggta
     1501 ccggatctac ctttcccgct ccgcggtggc gggcgacttt gtgcttttgg gctcgtacga
     1561 cttcatcgac tggcaggtct ggtacaaaaa cgaagggctt tccccggcgg tctggcaacg
     1621 gctgaaaacg gaaaagcggc cgcttaccct gccggagatc aaacagcagt tcggggccac
     1681 ggttgacccg gaagtggtca aagattcgtc ggtggccatc cggacgcacc gggggaccgg
     1741 tacacgggac acttcgtttt tcttccggcc ggaggactac aaccgggggt tggatccgat
     1801 tcgcgtctac cgggacacgg cggcggcgcg ccgggacttc ccgacgcttt caccggcggc
     1861 gctcgacagc ttgttcaacg actccctgaa atacgaatac cagtattctg cggatcacct
     1921 aatccccagc cgaccgattt tcgtgggggt cagcgctttt gactttggtg acgttgcgtc
     1981 cggactggat cctctggaat cgtcgccgca gtcaaacgcc aagttcgcgt tcccgctcgc
     2041 gaccatagac cgggtctttg ccgccaaaga gaaagtcaag gtatttccca acccgtacaa
     2101 gatcgatgcg gggtacggga ttttcgagca gccccagggc attcccgaac aggccaccga
     2161 atacgaccgc aaaattcact tcatcaacct gccccccaag tgcaccatcc gtattttcac
     2221 agtggatggc gacctggtgc gggagattca gcacgactac agcagccgtc ccttcgaccc
     2281 cctggcctcc tacgacgtct gggacgtgat cagccggaac acgcagtcgg tggtcaccgg
     2341 gctgtacctc tacagcattg aagctcagga tgtgcccgct ttcccgattc ctcccgagct
     2401 gaaacggcag gtcggcaaac tggtaattat gaaatagggg ccctattctt acaggagcga
     2461 accgatatga tgaagaaagc gaaggtactg tggctattga ccctggccgt tttgggaccg
     2521 cacctggcct ggggccaggc caaggtgggc actgcgggtg ctcagttctt ggaactcggg
     2581 gtatcggcac gcgccgcggc gatgggagat gctttcgtca gtctcgccaa tgacgccacc
     2641 gcgctctact acaatccggc ggggctgacc cagctttcgg gaaaacaggc ggtgttcagc
     2701 ggggtgcagt tgccggccga catgcaattg ggcttcgtcg ggtttgtgct cccggtggcg
     2761 aaactgggcg ggcagatggg tgtttcggtc tacgggctca actccgggga cattgaagag
     2821 accgactacg ggcacccctc cggcacgggc agaacttttg gggtgttcga tctggcggtg
     2881 gctttgagtt acgcccggag cttgaccgac cacttcagca tgggggtcac cgccaagttt
     2941 atccaggagt cgtttaacgg cgtcggcttt cggatcaccc ccagcggcgg gcaggaatca
     3001 attgacgaac attcacgcgg ctgggccgcc gacgtgggaa ctctgtacga caccggcttc
     3061 cggggtttcc ggattgccat gagcatcacc aacttcggtc ccgacctcaa actctccagc
     3121 aacgagttcc cgggcgaggc attcaagcgc caggcctacg ccttgccgat caacttcaaa
     3181 ttcggcaccg ccatcaacgt ggtggaactt cccaacaacc gggtgaccct ggccctggag
     3241 gggagccacc cttccgataa cttagagaag tttcagggcg gcgtggagta cgcgtggcac
     3301 gatatactgg ccttgcgcgc cggcgcgcgg gcacgcagca acgtgacgtg cgaacaaaaa
     3361 agcatcgctg ctttcgaccg gctgaccttt acctttggcg gcggcgtaca ggtgcccctg
     3421 ggtcagtacc ggttacaggc cgactacgcg ttccaggact tcggggttct ggataacatt
     3481 catcggctgt cggtcattct cagcttctga cgagcggtac cgttaaacaa accggactgg
     3541 cgaaaagagt agtggaggaa atttcatgaa taagcggttg cggatctgtc tggttgcggc
     3601 gggaggcggt gctctgggcc tggcgctttg gctctggggc tgtacgaaag gaaaaggagt
     3661 ttacccggat aacgttcctc cggtcatcgc gtttgtgaac atcccgccgg agagcacgta
     3721 ctattcgcag tcccccaccg tttactggta tgccaccgac ctggacggtt tcgtcaccca
     3781 gtaccagtac acgattgtga ccaaggccaa attgatggcc gacattctgg ggattgactc
     3841 ggcggtggcg gc
//
LOCUS       DBNGZA010000236         4702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1316503, whole genome shotgun sequence.
ACCESSION   DBNGZA010000236 DBNGZA010000000
VERSION     DBNGZA010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1316503"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="AC16FU_04890"
     CDS             <1..110
                     /locus_tag="AC16FU_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993742.1"
                     /translation="VTLVHEMQKRQARRGLATLCISGGMGLALLVESVS"
     gene            136..480
                     /locus_tag="AC16FU_04895"
     CDS             136..480
                     /locus_tag="AC16FU_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993743.1"
                     /translation="MAEWSDWYPLTYDSVQAESQDLPGVYQIALAEGELEYGSARSPI
                     IYIEATYRGTLRTQLLEEVEGSGSPCVFDHLSRQPLIYRELATAEPEQEEEAALAEFE
                     REFGTLPACNKQ"
     gene            493..1311
                     /locus_tag="AC16FU_04900"
     CDS             493..1311
                     /locus_tag="AC16FU_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2993744.1"
                     /translation="MTDKHVVLTTSGPIGTLTLNRPEKLNAFIDTMREEIAQGLEELG
                     NNTDVRVVVVTGAGRGFCAGADINYLAQLKRENDARNFKGLLEAGRRAITTIRNMDKP
                     VIAAINGPAAGGGLCLALACDLRIASDQATFGATFVKIGLHPDWAGGYFLPRLAGTAK
                     ALELMLTGEVINAQEAERIGLVNRVVPHDRLAAETKDLALKLSSRPANAVREIKNTAH
                     LSLDLSLEEMLDREFEVQMQMFHTPDMAEGVNAFLEKRTPVFGSAAGHSGRKEK"
     gene            1311..2273
                     /locus_tag="AC16FU_04905"
     CDS             1311..2273
                     /locus_tag="AC16FU_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR2993745.1"
                     /translation="MEQTTCPVQPAHAHRPKTAQVVQVSHGTHYHHRPVEQKRLKLAL
                     WLIGLTMIGECVGGFLTNSLALISDAGHMFTHFFALGVSFFAIRLASKKTGRDKSFGL
                     YRVEVLAALFNGITLVAITAYIFIEGVTRILHPQPVAELAMLGIAFVGLLVNLGSAVI
                     LWGASREDLNVRSAFLHMLGDTASSVGIVAAAIIISFTNWYILDPIASILIGAVILVW
                     AGRLLWDSVYVLLEATPRKIKVDEVITGLSEVEGVKTIHDVHIWEITSKMYSLTAHVV
                     IDDLRVSESQRIADRLSRLADQRFDICHTNFQFETFPQSESMLQ"
     gene            2344..3066
                     /locus_tag="AC16FU_04910"
     CDS             2344..3066
                     /locus_tag="AC16FU_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR2993746.1"
                     /translation="MQWRPDMKKLESPAEMSPEYRDLLITLLTVQCDTEFASVQQLRP
                     WVDMAPSINDRWAVSRVMMEEMRHGWQICQLLKSLAAEAVVQDLMWRKMGEHKLDSFN
                     IPFDEWSDVVLFNFLVDRVGLFQLTEFKDCSFGPLHRAVDTMLEEERFHISTGKAALA
                     KLCEEAEGRQQAQKAIYKWYPRALDMFGRAGSRRNEMARQFGIKKRKNEDARRDYVNE
                     VTPLIAELGLELPPEELNRRIL"
     gene            3141..3860
                     /locus_tag="AC16FU_04915"
     CDS             3141..3860
                     /locus_tag="AC16FU_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR2993747.1"
                     /translation="MTATIEKIETVDQMSPEYRDCLVGLLLVQADAELASVQLIRPWL
                     DQAPTVDDRWLLARRVTDAVQHAASIYRILKPLGAEAQMEQVLARKIGEHALDAFNMP
                     LETWPDLVLFSFLIDRVGVYQLREFVNSSYAPLARAVPKMVEEKTIHMHYGQNLLTAL
                     CQTDSGKKQAQAALRKWYPRALDMFGSAESKRNQQYRQYGLKKKTNEEARQSYVADVG
                     TILREQAGLEVPPPEFDRRIL"
     gene            3933..4688
                     /locus_tag="AC16FU_04920"
     CDS             3933..4688
                     /locus_tag="AC16FU_04920"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR2993748.1"
                     /translation="MDYSLSELMIAAAAREIHDPEIVFVGMRLPLLAFLVAKATHAPH
                     AIGLYENGIIRQKPAPELLYTMGDPPNVLGATFCGQMIDVMGFLQQGKVDVGFIGAGE
                     VDRFGNLNSSYVRDGNRLTRLPGSGGACDIASLAKRVVVILPHEKRRLKEQVDYVTSP
                     GYGTGNGWRRKVGLPHGGTSVVITTKGILRFEEQSGEAHLSSYHPGVTIEEIKKETGW
                     ALKVNQNLHATEPPDETELKIIRNYDPHRFWTK"
BASE COUNT         1038 a         1342 c         1387 g          935 t
ORIGIN      
        1 tcgttaccct ggtacacgaa atgcaaaaac gccaggcccg gcgcgggctg gcgacgctct
       61 gcatttccgg cgggatggga ctggcgctct tagtcgaaag cgtgtcgtag tttcgaaaaa
      121 tagcagggag gcgcgatggc ggagtggagc gactggtacc ctttgaccta cgacagcgtc
      181 caggccgagt cgcaggattt gcctggagtt taccagatcg ccctggcgga aggcgaactg
      241 gaatacgggt cggcccgcag ccccattatt tacatcgagg cgacctaccg gggcaccttg
      301 cggacgcagc ttttagaaga ggtggaaggc agcggcagcc cctgcgtctt tgatcacctg
      361 agtcgccagc cgttaatata ccgggaactg gccaccgcgg agccggagca ggaggaagaa
      421 gcggcgctgg cagaattcga gagggaattc ggcaccctgc cggcctgcaa taaacagtga
      481 ggcccgcaag ctatgaccga taaacacgtc gtcctgacca cctcgggtcc catcggcact
      541 ctgaccctca accgcccgga aaaactgaat gccttcatcg acacgatgcg cgaggaaatc
      601 gcgcaggggc tggaggagtt gggcaataat actgacgtgc gggtggtagt ggtgaccggc
      661 gcagggcggg gtttctgcgc gggagccgac atcaactacc tggctcagtt gaaaagagaa
      721 aatgacgcga ggaattttaa gggcttgctg gaagcgggac ggcgggccat aacgacgatc
      781 cgcaacatgg ataaaccggt gattgccgcg atcaacggcc cggccgccgg cggcggtttg
      841 tgtctggctc tggcctgcga cctgagaatt gcctcggatc aggcgacctt tggagcgacc
      901 tttgtgaaga tcggcctgca ccccgactgg gctgggggtt acttcttgcc gcgcctggcc
      961 ggcaccgcca aagcgttgga attaatgctc accggcgagg tgatcaatgc gcaggaggcc
     1021 gaacggattg gtctggtgaa ccgggtcgtc ccacacgacc ggctggcagc ggaaaccaaa
     1081 gatttggctc tgaaactctc cagtcgtccg gcgaacgcag ttcgggaaat taaaaatacc
     1141 gcccatctga gtctcgatct ctccctggaa gagatgttag accgtgaatt tgaagtccag
     1201 atgcaaatgt ttcacactcc ggacatggcc gaaggcgtga acgcatttct ggaaaaacgg
     1261 accccggtgt tcggttcggc cgccggccat tcaggccgaa aagaaaagta atggaacaga
     1321 caacctgtcc ggtacagccc gcacacgccc accggcccaa aacggctcag gtggtgcagg
     1381 tgagccatgg cacgcactac catcatcgac cggtggagca gaagcggttg aaactggccc
     1441 tctggctgat cggattgact atgatcggcg agtgcgtggg cgggtttttg accaatagtc
     1501 tcgccttgat ctccgacgcc ggccacatgt tcacccactt cttcgctttg ggagtgagtt
     1561 tcttcgccat ccggctggcc tcgaagaaaa ccggccggga taagagcttc ggcctctacc
     1621 gggtcgaagt tttggcggcg cttttcaacg gtatcacgct cgtggctatc accgcttaca
     1681 tcttcatcga gggggtcacc cgcatactcc acccccagcc ggtagcggaa ctggccatgc
     1741 tgggcatcgc ttttgtgggc ctcttagtga atctggggtc agcggtgatc ctctggggtg
     1801 ccagccggga agatttaaac gtgcgtagcg cctttctgca tatgctcggt gacaccgcct
     1861 cctcggtggg aatcgtcgcc gcggcgatta tcatttcctt caccaactgg tacatcctcg
     1921 atccgattgc cagcatctta attggtgccg tgattttagt ctgggccggg cggctgctct
     1981 gggattccgt gtacgtcctc ctggaggcga ccccgcgcaa gatcaaagtg gacgaggtga
     2041 ttaccggtct atccgaagtg gaaggcgtaa agacgataca cgacgtgcac atctgggaaa
     2101 ttacctccaa aatgtactca ctcaccgcgc acgtggtgat cgacgacctg cgcgtgtccg
     2161 aaagccagag aattgccgac cggctgagcc ggctggccga ccagcgcttc gacatctgcc
     2221 acaccaactt ccagtttgag actttcccgc aatcggagtc aatgcttcag taacgtcccc
     2281 ccttctgctg ctttacccgc aaatgctttt gtcatatgtt ctgcctcacg gaaggaggac
     2341 tctatgcagt ggcgcccgga tatgaaaaaa ttggaatcgc cggcggagat gtcgccggaa
     2401 taccgcgacc tcttgatcac gctattgacc gtgcagtgcg acaccgaatt cgcctcggtc
     2461 cagcagctgc gcccctgggt cgatatggcc ccctccatca acgaccgctg ggccgtctcg
     2521 cgggtgatga tggaagagat gcgccacggc tggcagatct gtcagttgct caaaagcctg
     2581 gctgccgagg cagtggtgca ggatttgatg tggcgcaaaa tgggggagca taagcttgat
     2641 tctttcaaca tcccgtttga cgaatggtcc gacgtcgtgc tgttcaattt cttagtcgat
     2701 cgggtcgggc tgttccagtt gaccgaattc aaagattgca gtttcgggcc gctccaccgc
     2761 gccgtggata ccatgttaga ggaagagaga ttccacatct ccaccggcaa ggccgccctg
     2821 gccaaactgt gcgaagaggc cgaaggccgt cagcaagcgc aaaaggcgat ttacaaatgg
     2881 tatccccgcg cgctggatat gttcggccgg gccgggtcaa ggcgcaatga aatggcccgg
     2941 cagttcggga tcaagaagcg caagaatgaa gacgcccggc gggactacgt caacgaagtt
     3001 accccgctga tcgctgaatt aggcctggaa ctgccgcccg aagagctaaa ccggagaatt
     3061 ctgtagacag cgggcaagga gatcgggttc tgcctccggt ttgtggccag atcattctct
     3121 ctccctgatc cgaaaaatca atgaccgcta ctatcgaaaa aatcgaaacc gttgaccaga
     3181 tgtcgccgga gtaccgggac tgcctggtgg ggctgctttt ggtgcaggcc gatgccgaac
     3241 tggcctccgt acagttgatc cgcccctggc tcgaccaagc accgacggtg gacgaccgct
     3301 ggcttttggc ccggcgggtc accgacgccg tccagcacgc cgcctcgatc tatcgcatcc
     3361 tcaaaccgtt gggagccgaa gcccagatgg aacaggtgct ggcccgcaaa atcggcgaac
     3421 atgcgcttga cgccttcaat atgccgctgg agacctggcc ggaccttgtg ctgttctctt
     3481 tcctcattga ccgggtggga gtgtatcagt tgcgcgagtt cgttaactcc agttatgccc
     3541 ctttagcccg cgccgtgccc aaaatggtgg aagagaaaac gattcacatg cactacggtc
     3601 aaaacctttt gaccgccctc tgccagaccg acagcggcaa aaaacaggcg caggcggcgc
     3661 tccggaagtg gtacccccgc gcgctggata tgttcggttc cgccgaatcg aagcgcaacc
     3721 agcagtaccg ccagtacggt ttgaagaaga agaccaacga ggaagcgcgg cagagctacg
     3781 tcgccgatgt cgggacaatc ctccgcgagc aagccgggct ggaggtgccc ccgccggagt
     3841 ttgaccggcg catcctctga aaaagtgtcg aggtgtcggc gtgtcgaagt taaaatccta
     3901 aaatcgaaaa aaaagttatt ccctcttgaa cgatggatta ctccttatcc gaattgatga
     3961 ttgccgccgc cgcgcgggag attcacgacc cggaaatcgt tttcgtcggg atgcgcctgc
     4021 cgcttttggc attcctggtc gccaaggcga cccacgctcc ccacgccatc ggcctctacg
     4081 agaacggcat catccgtcaa aaaccagcgc cggaactgct ctacaccatg ggcgacccgc
     4141 ccaacgtgct gggcgccacc ttctgcggcc agatgattga cgtgatgggt tttcttcagc
     4201 agggaaaagt cgatgtcggt ttcattggcg cgggggaagt agaccgcttc ggcaatctta
     4261 attcatctta tgtgcgcgac ggaaatcgtc tgacccgcct gcccggcagc ggcggggcct
     4321 gcgacattgc gtcattagct aaacgtgtag ttgtcatcct gccgcatgag aagaggcggc
     4381 tgaaagagca ggttgactac gtcacttccc ccggatacgg cacgggcaac ggctggcgaa
     4441 gaaaagtcgg actgccgcac ggcgggacat ccgtcgttat cactaccaaa ggcatcttac
     4501 ggttcgagga acaatccggc gaggcgcacc tctcttcgta ccatcccgga gttactatcg
     4561 aggagattaa aaaagaaacc ggctgggcgt taaaggtaaa tcagaatctc catgctaccg
     4621 aaccgccgga cgaaactgaa ctcaagatta tccggaacta cgatccgcac cgcttctgga
     4681 cgaagtagtg aacccgaaag ga
//
LOCUS       DBNGZA010000237         9727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2270959, whole genome shotgun sequence.
ACCESSION   DBNGZA010000237 DBNGZA010000000
VERSION     DBNGZA010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2270959"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="AC16FU_04925"
     CDS             <1..427
                     /locus_tag="AC16FU_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2993749.1"
                     /translation="NEGPDTWSPYDVVGHLIHGEKTDWIPRLEIILSADPDKRFEPFD
                     RFAQFAESKGKSLEQLIDEFQSLRKLNIEKLRLKKLNAADFAKPGLHPQLGTVTLQQL
                     LATWAVHDLNHIAQIVRVMAKRYRREVGPWTEYLKILRQ"
     gene            761..3223
                     /locus_tag="AC16FU_04930"
     CDS             761..3223
                     /locus_tag="AC16FU_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993750.1"
                     /translation="MPEVLLGVGIFLLALSPSLFGVGLFSFDLQGTPIPRLRANSAVD
                     IVRKGTPDTIWVGTGRGVSFTADSGRTWFTFDHTNGLSADEISALATDGIRLWVGTSH
                     SVVFGNQAVSFGDGFSVTTDNGFSWQSPQPQQAVGGAKVPFDIAFSNLPADTTIWSVA
                     FCGGLFRSTDFGTNWTNVYPDTTYKGTCELGQDNNPRDLNNRFFSVVADTNRPDTAVI
                     WAGSANGVNKFTYIRKRSRLAGNRIGALGFADSLLWLGTEVGLSTTNPFDSLHPFRTN
                     TTTPQLQNVGISAVFADSQKIIVATASAESLKAGIDLLISRDSGRTWQLRGDSLMVGL
                     NRVVTSIATADSILFVTAKSGGLLRSFNSGQSWQRIDSTGRSFFSVATQKIQPDTLVV
                     WAGGDSGLFKFVFGGAVDTLDTRTHYGVSDAAFLGNRVTLVKVFVDSGSVWAGVEPDS
                     LPQRFGLHRTRNDGANWDTSFVDQQAHDLAFAPYGFWLGTSGGLWRSINQGILNSWRQ
                     VTVKDTTTRDSLPGVEVFSLIPVANRLWVGTEVGLATTDSGAQFSIFRTGKGEADRIQ
                     RFTSASHGLSGNFITAMGIQRFSSQKILWAATQFAGGVETNALSFTKDDGKTWQVTLP
                     NVRAWNFSFADSVVWIATSQGLYHSFNLGQTWKRDTLVDFTDGTSISDLSEVFGVLAF
                     TTEVWVATDNGLAHSTDKGNTWRVIRAFKGIAPPPAPQESLTYAAPSPFSPLRSPGFC
                     SIHYRPEKTTQVTIRVYDFALNLVKTVTENQPRLAGVQYDDLDLWDGKNEKNDVVATG
                     VYFYQIDFADGQDLWGKVVVLK"
     gene            3220..4263
                     /locus_tag="AC16FU_04935"
     CDS             3220..4263
                     /locus_tag="AC16FU_04935"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993751.1"
                     /translation="MTAGEQQRPRQRRGSQGPVARKRQAIMTLVVGLLLIFGHKHPRA
                     ATGDGGYAAPFLQLGLGARAVAMGGAFSAIADDGTAPLWNAAASAWLVKRQFSATYRK
                     MFLDRSTGYVSILFPVRHEAAFGANWRFAGTDDIFGRDDVGDITEKIKNFENQFSVNF
                     ARRFTRALALGVNIKYLQQNLANINAFSVGFDLAAHFIPNRDLKLGKTVIYMRSLRIG
                     LAVENLGLKYPWNSAEYYSRFGQLGSSVTDNVPVNYKAGISYRFKRTPALLAVDVEKN
                     SRQRARSHFGTEYTAFRILALRAGVNRGDPTFGLGVRQPFRKAELGLDYAFVTRPVAG
                     ADPDHLLTLQIAF"
     gene            4263..5174
                     /locus_tag="AC16FU_04940"
     CDS             4263..5174
                     /locus_tag="AC16FU_04940"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR2993752.1"
                     /translation="MSLPRIYKFTFATFGFFCLIQTTSPSLHAESGTSSLVFLKIAPG
                     TRAVAMGEAATALESDLAAAYWNPAATVGLKSHHALFLQNFFILDVNYNYAGFGWGNG
                     KRALGTTLTLSNLSGIQRRGDNPLAEPLGAFELNDAALSFFYAREVREKVSVGGSAKI
                     LYEKIDLASDFTWALDLGGTYRLRPELTVGAAFANLGPKLRLQSESFRLPLRGSLGAA
                     YRWRQVLVTSDFVKFNDGSLKWHLGAEYSFEKYFALRSGWQLGYSDKNFSAGFGLKYQ
                     RFVVDYAFVPFQSDLGQSHRISLEVVF"
     gene            complement(5409..6398)
                     /locus_tag="AC16FU_04945"
     CDS             complement(5409..6398)
                     /locus_tag="AC16FU_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR2993753.1"
                     /translation="MSAEKSGITGQLLAPVEHPVSFLHWKIRAAWFLLWQKSRFARDF
                     LWETYRGTFFADNVPLLAAAISFYTVLSILPLALVFVAISGYVLKSSEQALFEATNFL
                     VKIFPATTTSVFTILLDLIRKKTIFGVVGLVGLAWTASRIFGVTEAAMNAVWQAPSGR
                     TWWRSKLLSVILIPLAFIFLVFSVTVTSLYRLAQHRQLPLVGHRISEFPTLGKIVTLL
                     LPIFLGFLAFWVAYRFLSNRKVPWRAAALGALVASLVWEACKYGFDLYIKRFADYNRV
                     YGTYATLVITVLWIYFSAYILLVGAEIGVHFENVRQRFRIFAFFRKAFGQGFS"
     gene            complement(6404..7462)
                     /locus_tag="AC16FU_04950"
     CDS             complement(6404..7462)
                     /locus_tag="AC16FU_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR2993754.1"
                     /translation="MTPPAKNLSLTAIALPPVAVAGATAFFYFAAPILVPLVCGVFAA
                     YLLGPAVGVLRHIKVPRVVAVFLVMLITILVLLVAGYFAGQQIGQLTGDLPHYWNAAV
                     QWLGQLQAKIAGWLQPKSTAPEPLNPVASLKWENLTSVSKIAFKGLGSVVNFFFGVIL
                     IFFITFFLLLDHERLKERAVRAFGRGNDEVAGKILSEINGQIAGFVAIKFVITVMLAV
                     VITAGLLLFRVKYAFVWGPVAAVLNLIPYIGPVIGLVPPVIVAGIQHQSALAAFWVFV
                     FFVAVQAVESNFLTPRLLSEKVNLNPLAVLVASLYWGWLWGAVGAVLAVPITAMVKVI
                     CDHVEALEPIGILLGGKK"
     gene            complement(7466..9490)
                     /gene="ligA"
                     /locus_tag="AC16FU_04955"
     CDS             complement(7466..9490)
                     /gene="ligA"
                     /locus_tag="AC16FU_04955"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR2993755.1"
                     /translation="MAEKTSGAKARAALLREKINYHNYRYHVLDKPEISDAEYDRLFD
                     ELVELEKAHPELVTSDSPTQRVGAAPAEQFKTVRHTLPMLSLGKASSEEEFLDFHRRV
                     LELSDQPESKIEYVCEPKLDGLAVELIYRDGIFVLGSTRGDGVAGEEVTQNLKTIKTI
                     PLRLLSDGKPVPRLLEVRGEVIIAKKAFWELNRKLEEAGEEPFANPRNFAAGQVRQLD
                     PRMTASRPLEIFAYGIGDLKGEKHRTHYETMQRLKELGLRINREIHLCRKPKDVMDYY
                     DKMLARRDKWDYEMDGIVIKVNDYELQRTLGELSRSPRWAVAWKFPPQQAETVIEEIK
                     VQVGRTGVLTPVAHLKPVHVAGVEVSRATLHNEDEIKKKDIRIGDTVVIERAGDVIPD
                     VVSVVTAKRTGKERHFKMPEKCPVCGSKVERLPGEVAYRCTGLACPAQLKERIAHFAC
                     KGGMDIEGLGFKWIEQFVEKRFISDPADLYFLTKEQLLTLDRMGPKLADNILNAIEKS
                     KRPDLPHLLFALGIRNVGEHLAGVLAREVGSIDNIAEQTIDDLTKIHEVGPIVAQSLY
                     NFFQDRNNQKVLAKLEKGGVVFPVEKRKLKQTVLTGKTFVLTGGLDSFTRDEAKKIIE
                     DMGGRVASSVGKKTDFVLVGKDPGSKFDDARRLGIRTIDEAEFKKMIGKG"
BASE COUNT         2046 a         2929 c         2676 g         2076 t
ORIGIN      
        1 caatgaaggg ccggatacct ggagtccata tgacgtcgtc gggcatctca tccacgggga
       61 aaaaaccgat tggatccccc ggctggaaat aatcctctcg gctgatcccg ataaaaggtt
      121 cgaacccttc gaccggtttg cccagttcgc cgaaagtaaa gggaaatctt tggagcaact
      181 tatagatgaa ttccaatctc ttcgcaaact gaatatcgaa aaacttcggt taaagaaatt
      241 aaacgcagcc gattttgcga agccgggctt gcatccccag ttggggacgg tgaccctgca
      301 gcagctgctg gcaacctggg cggtgcacga tttgaatcac atagcgcaaa tagtcagggt
      361 gatggcgaaa cggtacagga gggaggtcgg tccctggacg gaatatttga aaatccttag
      421 acagtagagc gatgctgttc acgagaaacc ttgcgcgaac accgtaaggt cgggagaccc
      481 cgccctgtcc tgattgggca tttcgtagcc gaggtttata acctcggcgg cccgtagggc
      541 cgtcgccttt tgcttgaagg tagaccggct cgtctccgag ccggtcgaaa ccaagtcggg
      601 ctcgagtcga tacaactcgt accgaggacg agcccatacg ggccgttccg gcccgactac
      661 cgctatgaac ctgcttttta ttgacaaaat cccccttacg cggtatcttt ctcgcgtggg
      721 aaaaccgcct cggtgcttct accgcaggtc ccgacggttt ttgccggaag ttctgctcgg
      781 cgtcgggatt tttcttttgg ctctctctcc ttcacttttc ggcgtcggcc ttttctcctt
      841 cgacctgcag ggcacgccca tcccccggct ccgggccaac tcggcagtgg acatcgtccg
      901 caaaggaact ccggacacca tctgggtggg cactggtcgg ggggtcagtt tcaccgccga
      961 cagcggccgg acctggttca cctttgacca caccaacggc ttgagcgccg acgaaatctc
     1021 cgccctggcg accgatggca tccggttatg ggtgggaacc tcgcactcag ttgttttcgg
     1081 caatcaggcc gtatcttttg gcgacggctt tagcgttacc accgacaatg gcttctcgtg
     1141 gcaatcgccg caaccgcagc aggccgtggg gggagcaaaa gtgccattcg acattgcctt
     1201 ttccaatctg ccggcggata ccacaatttg gtcagtcgct ttttgcggcg gcttgttccg
     1261 ctccaccgat tttggcacca actggacgaa tgtctatccg gataccacct acaagggaac
     1321 ctgcgagtta ggccaagaca acaacccccg cgacctgaac aaccgtttct tctcggtcgt
     1381 ggccgacacg aaccggcccg acacggcggt gatctgggcc ggctccgcta atggcgtgaa
     1441 caagttcacc tacatccgca agcgcagccg gctggccggc aaccgtatcg gtgccctagg
     1501 ctttgccgac agtttgctct ggctgggaac tgaggtggga ctttcgacaa ccaatccatt
     1561 tgactcactc caccctttcc gaacgaatac gactacaccg caactgcaaa acgtcggcat
     1621 ctccgccgtt tttgccgact cgcagaaaat cattgtcgcc acggcgtcag ctgagtcgct
     1681 gaaagccgga atcgatttgc tcatttcccg tgactctggc cgcacctggc aactgagagg
     1741 cgattccttg atggtcggcc tcaaccgcgt cgtcacctca attgcaaccg ctgattcgat
     1801 tctctttgtc acagcgaagt ccggcggact cctgcgcagt ttcaattccg gccagtcctg
     1861 gcaacggatt gacagcaccg gccggagttt cttctctgtc gccacacaaa aaattcaacc
     1921 cgatacgctg gtggtttggg cggggggcga cagcgggctg ttcaagttcg tcttcggcgg
     1981 cgcggtcgat acgctggaca cccgcaccca ctacggagtc agtgatgccg cctttctggg
     2041 caaccgggtg accctcgtga aagtttttgt cgattcggga tcggtctggg ccggtgtcga
     2101 gcccgactcg ctcccccagc gctttggcct ccaccgaacg cgcaacgatg gcgcaaattg
     2161 ggacaccagt tttgtggatc aacaggcaca cgatctggcc tttgctccct atggcttctg
     2221 gctgggcact tccggcggcc tctggcgcag catcaatcag ggaattttga actcctggag
     2281 acaggtgaca gtcaaagata ccactacccg tgattccctc cctggagtcg aggttttctc
     2341 tttaatcccc gtagccaacc ggctctgggt cggaaccgaa gtgggtttgg caactaccga
     2401 cagcggcgca cagttttcca ttttccgtac tggaaaggga gaggcggaca gaattcagcg
     2461 ctttacctca gcgagccacg gcctctcggg gaacttcatc actgccatgg gcattcagcg
     2521 cttcagcagt caaaaaattc tctgggccgc aactcagttt gcgggcggag tggaaacgaa
     2581 cgccctctcg tttaccaagg acgacggaaa gacctggcag gtgacgctcc cgaacgtgcg
     2641 ggcctggaat ttctcgtttg ccgactcggt cgtctggatt gccacaagcc aagggttgta
     2701 tcattctttc aatctgggcc agacctggaa gcgggacacc ttggtcgatt tcaccgacgg
     2761 cacctccatc agcgatttgt cggaagtatt tggcgtcctg gctttcacta ctgaggtgtg
     2821 ggtagccacc gataacggcc tggctcattc aactgacaaa ggcaatacct ggcgggtcat
     2881 ccgcgccttc aagggcatcg ccccaccccc ggcgccgcag gagtcgctca cctacgccgc
     2941 cccctctccc ttttcacccc tgcgcagtcc cggtttctgc tccattcact accggccgga
     3001 gaaaaccacg caggtcacca tccgggtcta cgactttgcc ctgaatctgg tcaaaaccgt
     3061 aaccgagaac cagccacgcc tggccggtgt gcagtatgat gatcttgatc tctgggacgg
     3121 gaaaaacgaa aagaacgacg tcgtcgccac gggcgtctat ttttatcaga tcgattttgc
     3181 cgacggccag gacctctggg ggaaagtggt ggtgctgaaa tgacagcggg cgagcagcag
     3241 aggccccgcc agcggcgcgg gagccagggg ccagtagcca ggaaaagaca agccatcatg
     3301 acgttggtgg tagggctgct actcatcttt ggacataaac acccgcgggc ggcaaccggt
     3361 gacggcggct atgcggcgcc ttttttgcag ctgggcctgg gcgcccgtgc tgtggccatg
     3421 ggcggggctt tctctgccat agcggatgac ggcaccgctc ccctgtggaa tgcggctgcc
     3481 tccgcctggt tggtcaagcg ccagttttcc gccacttatc gaaagatgtt tttggatcgc
     3541 tccaccggct acgtcagcat cctctttccg gtgcgccacg aggcggcctt tggcgccaac
     3601 tggcgctttg ccggcaccga cgacatcttc ggccgcgacg acgtggggga catcaccgaa
     3661 aaaatcaaga atttcgaaaa ccagttctcc gtcaacttcg cccgccgttt cacccgcgcg
     3721 ctggccttag gggtgaacat caaatacctt cagcagaact tagccaacat caacgccttc
     3781 tcggtcggct ttgacctggc cgcccacttc attccgaacc gcgatctgaa actggggaag
     3841 accgtcattt acatgcgttc cctgcgcatt ggactggccg tcgagaacct gggcctcaag
     3901 tacccctgga attcggcgga gtattactcc cgcttcggcc agctgggcag ttcggtgacc
     3961 gacaacgtgc cggtaaacta caaagccggg atttcctacc gcttcaaacg caccccggca
     4021 cttctggccg tcgatgtgga aaaaaacagc cgccagcgcg cacggagcca cttcggcacc
     4081 gaatacaccg ccttccggat tttggcgttg cgggccggcg tgaaccgggg tgatcccact
     4141 ttcggcttgg gagtgcgaca acctttccgg aaagccgagc tcggcttgga ttacgctttc
     4201 gtgacccgtc ccgttgcagg tgctgatcct gaccacctct tgactctgca aatcgctttc
     4261 tgatgtcatt gcctcggatt tacaagttca ctttcgcaac gtttgggttt ttttgtctca
     4321 tccagacaac ttctccttcg ctacacgccg aaagcggtac ttccagcctc gtctttttga
     4381 aaatcgcgcc cggcacccgc gcggtggcga tgggtgaagc ggccacagcc ctggagagtg
     4441 atttggctgc cgcctactgg aatccggcgg cgacagtggg gctcaaaagc caccacgccc
     4501 tttttttgca aaactttttc atcctcgacg tgaactacaa ctacgccggc ttcggctggg
     4561 gaaacggaaa gagagcgctg ggcacgacgc tcaccttgag caacctctcc ggcatccaaa
     4621 ggcgcggcga caaccctttg gccgaaccct tgggcgcttt tgaattgaac gacgcggcgc
     4681 tctcgttttt ctacgcccgg gaagtgagag aaaaagtttc tgtcgggggt tccgccaaga
     4741 ttttgtacga aaaaatcgat ctcgccagcg acttcacctg ggccttggac ctcggcggaa
     4801 cctatcggct gcgccccgaa ttaaccgtcg gcgccgcttt tgccaactta ggacccaaac
     4861 ttcgtctgca gagcgaatcg ttccgcttac ccctgcgcgg ctccctgggt gccgcctacc
     4921 gctggcggca ggtactggtg acgagtgact tcgtgaagtt taacgacggc agtctcaaat
     4981 ggcatctggg cgccgaatat agttttgaaa aatatttcgc cttgcgcagc ggctggcagt
     5041 tgggctactc ggacaaaaat ttcagcgccg gtttcggcct caagtaccag cgcttcgtcg
     5101 tcgattacgc cttcgtcccc ttccagtccg acttgggtca gagccaccgc atttccctcg
     5161 aagtggtgtt ttgaaatttc cctaatttcg tagccgaggt ttttaacctc cgcggctcgg
     5221 aaagagccgt cgttgtttgg accacgctcg ggcggacgcc gagggcgttt tcccggttcg
     5281 gaaatttagt caggctggaa gcctgaccta tgggagaaga gatctttacc tccatagccg
     5341 aggcttccag cctcggaact gccgcttttg tagcggggag ccctacgggc cgtcgccatt
     5401 tgttacgtct atgaaaaccc ttgcccgaag gctttacgga aaaaagcaaa aatccggaag
     5461 cgctggcgca cgttctcaaa gtgcacgccg atttccgccc ccaccaaaag gatgtacgcc
     5521 gagaagtaaa tccagagcac ggtaatgacc aaagtggcgt aggtgccgta gacccggttg
     5581 tagtcggcga aacgtttgat gtagaggtcg aaaccgtact tgcaggcctc ccacaccagg
     5641 gaagccacca gtgctcccaa agcggccgcc cgccagggaa ctttccggtt cgacagaaac
     5701 cggtaggcga cccagaacgc cagaaacccc agaaaaatcg gcagaagcag tgtcacgatt
     5761 ttccccaaag tcgggaattc ggaaatccgg tggccgacca aaggcaactg ccggtgctgg
     5821 gccagccggt aaagcgaggt aaccgtcacc gagaaaacga gaaaaatgaa cgccagcgga
     5881 atgagaatga ccgagaggag cttgctccgc caccaggtgc ggccggacgg cgcctgccag
     5941 accgcattca tcgccgcttc ggtgaccccg aaaatgcgcg atgcggtcca ggccagtcca
     6001 accagaccga ccaccccgaa gatcgtcttc ttccggatta ggtcgagcaa aatcgtaaag
     6061 accgaggtcg tggtggccgg gaaaattttg accaggaaat tggtggcttc aaagagcgcc
     6121 tgttccgagg acttgagcac gtatccggaa atggcgacga agaccagagc cagagggaga
     6181 atggaaagca cggtgtagaa ggaaatcgcc gccgccaaaa gcgggacgtt gtcggcgaag
     6241 aacgttcccc ggtaggtttc ccagagaaaa tcgcgggcga agcggctctt ctgccagagc
     6301 aaaaaccagg cggcccggat tttccagtgt aaaaacgaaa ccggatgctc caccggagcc
     6361 aaaagttgac cggtgatgcc gctcttttcc gctgacataa gtgttacttc ttgccgccta
     6421 agagaattcc gatcggctct aaagcttcga catggtcgca gatcactttg accatggcgg
     6481 taatcggtac cgccagaacc gcgcccactg ccccccagag ccagccccag tagagactgg
     6541 cgacgagcac cgccagagga tttaagttca ccttttcgct gagtaacctc ggggtcaaaa
     6601 agttactctc cacggcctgg accgcaacga agaagacgaa cacccagaaa gccgccaagg
     6661 ccgactggtg ctgaatgccg gccacaatca cgggcggcac caggccgatc accgggccga
     6721 tgtagggaat caggttcagg accgccgcca cggggcccca aacgaaggcg tatttcaccc
     6781 gaaaaagcaa aagtccggcg gtgatgacca cggccagcat gaccgtgatg acgaacttga
     6841 tcgcgacgaa gccggcaatc tggccgttga tttcggaaag aattttgccc gccacctcgt
     6901 cattcccccg gccaaaggcg cggacggcgc gctccttgag ccgctcgtgg tccaagagca
     6961 aaaagaacgt gatgaagaaa atgaggatga ccccgaagaa gaaattcacc accgaaccca
     7021 gccccttgaa ggcgatcttg ctcaccgaag tcagattttc ccacttgagc gacgcaaccg
     7081 gattgagcgg ctcaggtgcc gtgcttttcg gctgtaacca gccggctatt tttgcctgga
     7141 gttggccgag ccactggacc gcggcattcc agtaatgtgg caaatcgccg gttaattgcc
     7201 cgatctgctg acccgcgaag taacctgcta ccaaaagaac cagtatggta atcagcatga
     7261 ccaggaagac ggcaaccact cggggaactt tgatgtggcg tagcaccccc acggcgggac
     7321 cgagaaggta cgccgcgaaa acgccgcaga cgagcggaac taaaatcggc gccgcaaagt
     7381 agaagaacgc cgtggccccg gccaccgcta ccggagggag cgcgatggcg gtcaaggaca
     7441 aattcttggc gggaggagtc atagcttacc cctttccgat cattttcttg aactctgctt
     7501 catcgatggt gcgaattccc agccgccggg catcgtcgaa cttggagccg gggtccttgc
     7561 ccactaacac aaagtcggtt tttttgccca ccgaggaagc cacccgcccg cccatatcct
     7621 ctatgatttt tttcgcttca tcgcgggtga acgaatcgag cccgccggtg aggacaaacg
     7681 ttttgcccgt gaggacggtt tgcttgagct tccttttctc gacgggaaag acgactcccc
     7741 ccttttctaa ttttgccaaa accttctggt tgtttctgtc ctggaagaaa ttgtagaggc
     7801 tctgtgctac aatcggtccg acttcgtgaa tctttgtcaa gtcatctatc gtttgttccg
     7861 cgatattgtc gattgacccc acctcgcggg ctaaaacccc ggccagatgc tcgcccacgt
     7921 tacgaattcc cagagcaaac agcaaatgcg gcaggtcggg ccttttactt ttctcgattg
     7981 cgttgaggat gttgtccgcc aacttgggac ccatccggtc gagcgtgagg agttgctcct
     8041 tggtgaggaa atagagatcc gccggatcgg agatgaatct tttctccacg aactgctcta
     8101 tccacttaaa acccagacct tcgatatcca ttccaccttt gcaggcgaaa tgagcaatcc
     8161 gctccttcag ctgcgccggg caggccagac cggtgcagcg gtaggccacc tctcccggca
     8221 gccgctcgac tttggagccg cagaccgggc atttctccgg cattttgaaa tgacgttcct
     8281 tgccggtgcg cttggcggtg acgaccgata ccacgtcggg gatcacgtct ccggcccgct
     8341 cgatcaccac cgtgtcgccg atgcgaatgt ccttcttttt gatttcgtcc tcgttgtgga
     8401 gcgtcgcccg gcttacctcc accccggcca cgtgcactgg cttcagatgc gccaccggcg
     8461 ttagaacgcc ggtccgcccc acctgcactt tgatttcctc aatgaccgtc tcggcttgct
     8521 ggggcgggaa cttccaggcc accgcccagc gcggtgagcg cgataactcc cccaacgtgc
     8581 gctggagttc gtagtcgttc actttgatga cgatcccgtc catttcgtag tcccacttat
     8641 cccgccgcgc gagcatcttg tcgtagtagt ccatcacgtc cttcggcttg cggcagaggt
     8701 gtatctcacg attgatcctc agtcctaatt ctttgagccg ttgcatcgtc tcgtagtgcg
     8761 tccggtgctt ttcgcctttc aggtcgccga tgccgtaggc gaaaatctct aagggccggc
     8821 tggcggtcat ccgggggtcc agctggcgca cctggcccgc ggcgaagttg cgcggattgg
     8881 caaacggctc ctctcccgct tcctcaagtt ttcgattcaa ctcccagaac gcttttttgg
     8941 cgatgatcac ctcgccgcgc acttcaagca atctcggaac cggcttcccg tcggagagga
     9001 ggcgcagggg gatggttttg atcgtcttga gattttgcgt cacctcctcg cctgccaccc
     9061 cgtcgccccg ggtggaacct aaaacgaaga taccgtcgcg gtatatcaac tccaccgcca
     9121 gcccgtctaa cttcggttca cagacgtact cgattttact ctccggctgg tcagataatt
     9181 ccagaactcg gcggtggaag tcgaggaatt cttcttcgga gctggccttg cccaaggaga
     9241 gcatcggtag ggtgtggcgc accgttttga actgctccgc cggcgccgcg ccgacccttt
     9301 gggtggggga atcggacgtg accagttccg gatgggcttt ttccaactcg accagttcat
     9361 caaaaagtcg gtcgtattcc gcgtcgctta tttccggttt gtccaggacg tggtaccggt
     9421 aattgtggta gttgattttc tcgcgcagca gggcggcccg cgccttggca cccgaagttt
     9481 tctcagccat tggaatctaa cagtaattaa cagccgctcg acggggtgtc aaacattgag
     9541 ccgtaatgac cgccagagta tattcacgac ttcatgacct tcttcagaac tctgctctgc
     9601 accaagtgcg gccaggagca caacccttcc cgcccccaaa acctctgccc ctgcggcggc
     9661 ccgctctacg cgcagtacga cctggcggcg attgcgaaaa aactcaagaa aagcgaactg
     9721 ccgagcc
//
LOCUS       DBNGZA010000238         3884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1103779, whole genome shotgun sequence.
ACCESSION   DBNGZA010000238 DBNGZA010000000
VERSION     DBNGZA010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1103779"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1298
                     /locus_tag="AC16FU_04960"
     CDS             387..1298
                     /locus_tag="AC16FU_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR2993756.1"
                     /translation="MRNRSSVWALGLAGLLALAFVGWALAGSRPKEISGENIGSEKCL
                     ACHEEVQAEYAHTVHFGVEENAQTELEKHMCEACHGPGAAHAEKPEDKSLHLTFKAKD
                     GESPDDWNRVCLQCHERGVQTHWAGSVHETRGVACVNCHTVMKNISREKQLAKETAPE
                     VCFTCHKMKKMQFQKSSHMPLVEGKVSCVDCHNPHGSPNPSMVIENSVVENCTKCHAE
                     KSGPFLWEHPPVRENCLNCHNPHGSNHDNLLIVKRPRLCQRCHVEARHPTTPQSEATL
                     FAIDRGCVNCHSQIHGSNHPSGMQFLR"
     gene            1312..3456
                     /locus_tag="AC16FU_04965"
     CDS             1312..3456
                     /locus_tag="AC16FU_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HIR2993757.1"
                     /translation="MKTLILFLATVTLAATAWTQEAVRTTGQLEGGFWQVLKNPGSAK
                     FQEYRQVKEDLLLNRFGLDLTKGNYYFGVNANELGKTDQGAVVRFGLVRKWKNNVSWS
                     QTPHLLSTTTREFFVNAGGGVFVVSDSIQRRLQSVPGSLKSFLEGAQPRNLFFQRNTA
                     SATTEFKPRSDITFKAGYTNERKQGSKQLGVTLFFNPVELPEPLNYETHRLSASAELA
                     RKNFSAAVGYSGRIFNNKTEVVIRDNLFRVTTDTATAPLRVRLALPVDNVAHNFTFTG
                     AYNLPKQTRSIATVSYGVATQNEPFLPHTINSAITDSGLALPAASLDGKFKTLLVNYQ
                     VSSRPVEKLSLKGKIRYYEFDNQTPSLDFGSYVAYGDYSLQTRGRENLPYGYIKRDYG
                     GEAGYQIVRQANASFAYTRENMTRNHREVLSSAEDIFKGTLDVRPSVWLTFRGSYSHA
                     ERKSQKYDSLFFEESFPEGEAVTNAGMSLNELRRFDVYNRNRDNVELSSQISPIDELD
                     VGLSFSLAQDDFANSAYGLEALWNRGWSMDISYSPTPRLTLLGDLGREDGRGEMESRY
                     RPVYANVGYDSLNNDWSGALRDRFENYGFGFSAEVWPKKLSWATNYGFTYSKSALLSS
                     FAPGGNPSGNAVSFPNVYNKFHRVASQVTYRLTSQTALKFGYLYEKYLETDWAIDPLQ
                     PFMAAASRSVFLGARVPGYEAHVVALKLSYNF"
BASE COUNT          905 a         1088 c         1085 g          806 t
ORIGIN      
        1 cgtccagcgc tacctcaagc gccagacggt ggccgactta gccaaaacgg tggcggagaa
       61 aaaaaagctg gtctccaaga gcaaatctcg gtcttaagct aggctgaacg tgctatcaaa
      121 acagggctgt tgcgttcagc gtccgagggt tctcgaatac ccatctgcgc ccattctcca
      181 atgggataag aaaagtcccg ctgactgggg ggcggggggc agcaaaccgg cccttgacag
      241 ggggaaatgc gctgcgcatt atattccaaa atggtcttgg tcatggagaa ttctaaccgg
      301 taatatttca taccaaaagg tcgataatgt aattgaggat gtctgacgtg tgggacagga
      361 agggaagcgt gctgagggag ggagccatgc gaaataggtc ttcggtttgg gcgctcggct
      421 tggccgggct cctggctctg gcgttcgtcg gctgggcgct ggccgggtcg cggcccaagg
      481 agatttccgg ggaaaacatc ggcagtgaaa agtgtctcgc ttgccatgag gaagtccagg
      541 cggagtatgc gcacaccgtc cattttggcg tggaagagaa cgcgcagacc gagttagaga
      601 agcatatgtg cgaagcctgc cacggccccg gtgcggccca cgcggagaag ccggaggata
      661 agagtttgca cctcacgttc aaggccaaag acggggaatc gcccgacgat tggaatcgag
      721 tatgcctgca gtgccacgag cgcggcgtgc agacgcattg ggccgggtcg gtgcacgaga
      781 cgcgcggggt ggcctgcgtc aactgccata ccgtgatgaa aaatatctcc agggaaaaac
      841 agctggccaa ggaaacggcg ccggaggttt gttttacctg ccacaagatg aaaaagatgc
      901 agttccagaa atcttcccat atgccattgg tggaaggcaa ggtttcttgc gtggactgcc
      961 acaatccgca cggctccccc aatcccagca tggttatcga aaactcggtg gtcgagaact
     1021 gcaccaaatg ccacgccgaa aaaagcggcc cctttttgtg ggagcatccg ccggtgcggg
     1081 agaactgttt gaactgccac aacccgcacg gctccaatca cgacaacctg ctgatagtca
     1141 agcgcccgcg gctctgccag cgctgccacg ttgaggccag gcacccgacc actccccagt
     1201 ccgaggccac actctttgcc atcgatcgcg gctgcgtcaa ttgtcattca caaattcacg
     1261 gttccaacca tccctccggg atgcaatttc tacgataggg aggcgaaggc gatgaaaact
     1321 ttgatcctgt ttctcgcgac ggttactctg gcggcgacgg cctggacaca ggaagcagtt
     1381 cggaccaccg gccagctgga ggggggattc tggcaggttc tcaaaaaccc cggctccgcc
     1441 aaatttcagg aataccggca ggttaaagag gatctgctgt tgaaccgctt cggccttgac
     1501 ctcaccaagg gcaactatta cttcggggtc aacgcgaacg aactggggaa aaccgaccag
     1561 ggagcggtgg tcagatttgg actggtccgg aaatggaaga ataatgtttc ctggtcgcag
     1621 accccgcatc ttttgtccac caccacgcga gagttcttcg tcaatgccgg cgggggcgtc
     1681 tttgtcgttt ccgacagcat tcagagaaga ctgcagtccg ttcccggcag tctgaagagc
     1741 ttcctcgagg gcgctcagcc gagaaacctc ttcttccagc gcaacacggc cagtgccacg
     1801 actgaattca aaccccgctc ggatataacc tttaaggccg gttacaccaa cgaaagaaaa
     1861 caggggagca agcaactggg agttactctt ttcttcaatc cggtggaact cccggagcct
     1921 ttgaactacg aaacccatcg cctttccgcc tcggcggaac tggcgcgcaa gaacttttcg
     1981 gcggcggtgg ggtacagcgg gcggattttc aacaacaaga ccgaggttgt gattcgggat
     2041 aacctcttcc gggtgacgac cgatacggca accgcccccc tacgcgtgcg gttggcgctg
     2101 cctgtggaca atgtggcgca caacttcact tttacgggcg cctacaatct tcccaaacag
     2161 acacgcagca tcgcgacggt aagctacggg gtggccacgc agaacgagcc ctttttgccg
     2221 cacaccatca acagcgccat tacggacagc ggcctggcct tgccggccgc cagtctggat
     2281 gggaaattca agaccctgct ggtcaactac caggtcagtt cccggccggt cgaaaaactt
     2341 tcgctgaaag gaaagattcg ctactacgag ttcgacaacc agacccccag cttggacttt
     2401 ggcagctatg tcgcgtacgg cgactacagt ttgcaaaccc gcgggcgtga aaaccttccc
     2461 tacggttata ttaagcggga ttatggcgga gaagccggct atcaaatcgt ccggcaggcg
     2521 aacgcgagct tcgcctacac ccgcgaaaat atgacccgca atcatcggga agtgctatca
     2581 tcggcggagg atatcttcaa ggggaccctg gatgtccgcc cgtccgtctg gctcactttc
     2641 cgcggctctt attcccacgc ggaacggaaa agccaaaaat acgattcgct tttctttgag
     2701 gagtctttcc cggaggggga ggccgttacc aatgccggca tgtctttgaa cgaattgcgc
     2761 cgctttgacg tgtacaaccg caaccgggac aacgtcgagc tctccagcca aatttctccc
     2821 atagacgagt tggatgttgg attaagcttc agcttggcgc aggatgactt tgccaattcg
     2881 gcatacggtt tggaagccct ttggaaccga ggctggagca tggacatttc ctactctccc
     2941 accccccgtc taaccttgtt gggagacctc gggcgggaag acgggcgggg agaaatggag
     3001 tcccgctacc gcccggtcta tgccaacgtc ggctacgact ccctgaacaa tgactggagc
     3061 ggcgctttgc gagaccgctt cgaaaactac ggctttggat tctccgccga ggtctggccc
     3121 aaaaagctct cctgggcaac gaattacggc ttcacctaca gcaaaagcgc cctgctctct
     3181 tcgtttgctc ccggcgggaa ccccagcggc aacgcggtca gtttccccaa cgtgtataac
     3241 aagttccatc gcgtcgcttc gcaggtgact tatcggctca ccagtcaaac cgccctgaaa
     3301 ttcggttacc tgtacgagaa ataccttgaa accgactggg cgattgaccc gctgcagccc
     3361 tttatggctg ccgccagccg ctcggtcttc ttaggcgcac gagtgcccgg ctacgaggcg
     3421 cacgtcgtgg cgctcaagtt gagttacaac ttttagattt tttgagggtt tttctggagt
     3481 gtccgggttg ccataaaaga cccggacact cttttttcat ccccccgatt tttatttttc
     3541 cgttttcagt aacttatcta ttgataggac attaccagcc aatttgaaga ggggctttaa
     3601 tgtgaaagtt ttcacaaaaa agacttgact acctgaattt ggtcctatat atacttggca
     3661 gaaagttagg gactaaaaag atatagtaac ctgattacag aagagggaac caggaaagca
     3721 gcggccaacc accaaaggag ggtacgatgc gccgtaagat ggtcatcacc gcagccacgc
     3781 tcgtcgcttt ttgggcgatc tggtcgttcc acaacgacct gaccgccatg acccagttcg
     3841 cgcggaagac cgggcttgag tgcaccagct gccacacggt catc
//
LOCUS       DBNGZA010000239         8450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1316931, whole genome shotgun sequence.
ACCESSION   DBNGZA010000239 DBNGZA010000000
VERSION     DBNGZA010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1316931"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1218
                     /locus_tag="AC16FU_04970"
     CDS             40..1218
                     /locus_tag="AC16FU_04970"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR2993758.1"
                     /translation="MTDRVILIVLDACGAGELPDARAYGDTGSNTLGNVARAVGGLHL
                     PHLERLGLGKIIPIEGVSPAVVAEGCYGKLAERSPGKDSTTGHWEIAGIFLEKPFPVY
                     PHGFPPEVIGPFEQAVGRKVLGNKPASGTEILKELGAEHHRTGRPIVYTSADSVFQIA
                     AHEEVIPLGELYQMCQTAREILAGDHAVGRVIARPFVGATPENFKRTANRRDFSLPPP
                     APTVLDQLKAADYPVIAIGKIEDLFAGQGVTQAVHTRDNAEVMQAMTQAVAETGRGLI
                     FANLVDFDTIWGHRNDVPAFAGGLERFDRHLGELIPRLRPDDLLIITADHGCDPTTPS
                     TDHSREYVPLLVYGQRLKSDVNLGTRSSFADVGATIAEIFRLPGTGNGKSFWQEIAAA
                     "
     gene            1181..1588
                     /gene="cdd"
                     /locus_tag="AC16FU_04975"
     CDS             1181..1588
                     /gene="cdd"
                     /locus_tag="AC16FU_04975"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR2993759.1"
                     /translation="MAKVSGRKSPRPDRLFLAARHAFRRAYAPYSKFPVGAALEGKRG
                     EVFAGCNVENSSYGLTVCAERVALFSAIARGERSFTRLAIVTRSRTPVTPCGVCLQVL
                     AEFCDDLEIISATTSGKKATYRLRELFPKPFRR"
     gene            1619..2416
                     /gene="deoC"
                     /locus_tag="AC16FU_04980"
     CDS             1619..2416
                     /gene="deoC"
                     /locus_tag="AC16FU_04980"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR2993760.1"
                     /translation="MIEQVLLETWRTDLSRTLGIDFPPLSQNSGRSVAFSPAEFHSLP
                     APEKRKYLTGVSDYALLKPDLTSPVLEEACSRARELGCFGFCSLPYFVPQARKFLEGS
                     PVHLISVVGFPWGVHPPQTKQKETEWLAAEGVAEIDFVTNTSAIKKGDYATVWEEVRL
                     IRQTFPLGILKGIIECPLLTTEEKVKTAWLVEQAGCQFVKTGSGLFGKVQVADVELLR
                     RILLPATNIKAAGGIRTLEDALPLLEAGAERLGTSTPDTILAEISNA"
     gene            complement(2598..5054)
                     /locus_tag="AC16FU_04985"
     CDS             complement(2598..5054)
                     /locus_tag="AC16FU_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR2993761.1"
                     /translation="MASRFLCLLALAFLLTPATLFPFEPLFDTWLSYDVGVYPSAVIA
                     SDLDGDGDQDLVIPSYDGLFLFSVLKNNGDGTFAPRTDYRIGRYIYAVVASDLDRDGD
                     QDLAVTISDSNAVAVLMNYGDDTFAVRVNYDAGYRPEAISAVDIDDDGDVDLVIANTG
                     HTTNQGKTVSVLKNNGDGTFAPRVFYGTGNGPTSICAPDLDGDGHPDLVTADYFSRSI
                     SVLKNNGDGTFALKIDYATGNAPSAVFASDFDNDGDQDLAVTNDSSQSVSIFKNNGDG
                     TFAPKVDYETGNRPRSVFVADLDKDGDKDLAVANMLSNTVSLFLNNGDGTFAPKRDYV
                     VGWNPSSIFCADVDGNGSPDIVASSISSSTVSVLKNKGDGTFFVQTNYAAGGEPSPIL
                     AADLDRDGDQDLVVANSESSTVSVLKNNGDGTFAGRVDYGTGSKPISIFASDFDEDGS
                     QDLAVANAWSYTVSVLRNLGDGTFAEKVDYGTGSTPPFSVFASDLDGDGDPDLAVANR
                     GNTVSVFMNNGDGTFAPKIDYRTGTGPYAVLACDIDGDGDQDLVTADNTLDVGYLSVL
                     KNNGDGTFASPVNYPSAIEPNAIFASDLDRDGDQDLITAGWTVSVSMNNGDGTFPMKV
                     DYGRYYFSSIFVQDFDGDGDPDLVAPNSGNAVSIFKNKGDGSFSNRADYGTGNAPSAV
                     SASDFDKDGDIDIVTANYLGNSVSVLKNISTPTKVKDNGDKPLPSSWVLQQNHPNPFN
                     PSTIIRFAVPQPGNMTLEIFNLLGQRVKTLVNEVVSAGEKEVTWDGTDDRGKEVASGI
                     YFYRLKAEDFMETKKMVLFK"
     gene            complement(5189..7813)
                     /locus_tag="AC16FU_04990"
     CDS             complement(5189..7813)
                     /locus_tag="AC16FU_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR2993762.1"
                     /translation="MQFQRYGILAFVGVVTAALFLSTAPPRFGVSATPGVKERLRKAG
                     ATAELQRLLNVEHLEALISSEGADPSKGYRLRPSTLAGITATDTIRVLFVLVDFPDRP
                     ASAGAVTTPTMFDSLLLSTGHKNPTGSYTEFYMENTNGKLFIKGDVIGWYRMDSTNAY
                     YCNGASGLAPNGRARQLARDVALKVDPDVDFTLYDNDGNGTVDGFYVVHAGIGAEETG
                     SGNDLWSHVSNISPSVPVDGKTVSRYTVCPEENSGNRMVAIGVFCHESGHLLFFLPDL
                     YVDSNPGGEGVGEWSLMGSCNWLNNSKTPCHMDPWCKFQRRWAEIETVTTNQNAVSIP
                     KTVGTNKAYRLWTNGSLGTSYFYVENRRRTGFDAALNGEGLLIWQINESQSQGNNPPK
                     VDLLQADGLRNLENGNNRGDAGDPFPGTSNNRNFDEFSNPNSKNPFNQEATQVAVFNL
                     GNGGLDTMTANFEVTYGRPHLSIDSTWFDDAATGNNNGKIDSAETVRFYVRLKNAWAN
                     ASNVTVLLRADDPRIVFADSTALLASVPGLGATTTSLSDPIEFTADPSLPNSYTIFFT
                     VLAWNNDSSATVQQTFKATVGPTTILLVDDDGGTNNWESFYTTTLDSVRKPFGSHTVL
                     TGGIPSIPTLSSYPTVIWFTGNNRTPTTLTSTAVANLKAYLDGGGKLFITGQSIGEAL
                     AASADSSFLINYLHCRYGGNTFTIRANGVTGDPISNALDVYILGADGAANQTSADWLK
                     LQPGADSIFTYLSNSKPAGVRVIKDTTYKLVYLGFGFEGISSTRVGFATRQDVMTKVL
                     NWLNAPYPVIILGDFDNSGVVDITDIVGTVAYVVFGTPGPVNGQASIDVNCDGVIDLV
                     DIVAMIQNVVFSLPFSCP"
     gene            complement(8027..>8450)
                     /locus_tag="AC16FU_04995"
     CDS             complement(8027..>8450)
                     /locus_tag="AC16FU_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993763.1"
                     /translation="AYNPEQGLGISDLVFDYTGVALAVAKARSQALQNFDVKISFRGN
                     PWAENHHFFSQNARDFDRILYWITYRPEKLARGIMIVGLGYQTDHLPPDYRVTRAFVI
                     GLGTTLPDLLRPIAPGLARRMAPLGIYFLNLRCSAVRW"
BASE COUNT         1860 a         2357 c         2301 g         1932 t
ORIGIN      
        1 aagttccgct cggcggtgca gggtgagcga agccgaacca tgactgaccg ggtcatactc
       61 atcgtactgg atgcctgcgg cgccggcgaa ctgcccgatg ccagggctta cggcgatacc
      121 ggttcgaaca ccctcgggaa cgtcgcccgc gcggtgggcg gcctgcacct cccccatctc
      181 gaacgcctcg gcctggggaa aattatcccc atcgaggggg tcagtccggc ggtcgtcgcg
      241 gagggttgct acgggaagct ggcggagcgc tcgccgggaa aagacagcac caccggccat
      301 tgggaaatcg ctgggatttt tttggagaaa ccctttcccg tctacccgca cgggtttcct
      361 ccagaggtta tcggaccttt cgagcaggcg gttggcagaa aggttttggg gaacaaaccg
      421 gcctccggca cggaaatctt aaaagagttg ggcgcagaac accatcgcac ggggagaccc
      481 atcgtctaca cttcggcgga cagcgtcttt cagatcgccg cgcacgaaga ggtcattcct
      541 ttgggagagc tgtatcagat gtgccagacc gcgcgggaga ttctggcggg cgaccacgcg
      601 gtcggtcggg tgattgcccg gccctttgtg ggggctacac cggaaaactt caagcgtacc
      661 gccaaccggc gcgacttttc tctcccgccg ccggcaccta ccgtgctcga tcagctcaaa
      721 gctgcggact atccggtcat cgccataggc aagatcgaag acctctttgc cgggcagggg
      781 gtaacgcagg cggtacacac ccgggacaac gctgaagtga tgcaggcaat gacgcaggcg
      841 gtggccgaga cgggacgcgg gctcatcttt gccaatttgg tcgatttcga taccatctgg
      901 ggtcaccgca acgacgtgcc ggcttttgcc ggaggtctgg agcgtttcga ccgccactta
      961 ggcgaactca tcccccgcct gaggccggat gatctcttga ttatcacggc cgatcacggc
     1021 tgcgatccca ccactccttc caccgaccac tcgcgggagt acgtgccgct tttggtctac
     1081 ggccagcggc tcaaaagcga cgtcaacttg ggaaccagat cctcttttgc agacgtggga
     1141 gcgaccatag cggaaatctt tcgtcttccg gggaccggca atggcaaaag tttctggcag
     1201 gaaatcgccg cggcctgacc ggctgtttct ggccgcccgg cacgccttcc gccgggcgta
     1261 cgctccctac tcgaagtttc ccgtcggggc ggcgctggag gggaaaaggg gggaggtctt
     1321 cgcgggctgc aatgtggaaa attcctccta cggcttgacg gtttgcgcgg agcgggtggc
     1381 tcttttctcc gcaattgctc ggggtgaacg ctcgtttacg cgcctggcca ttgtcactcg
     1441 cagccgtact ccggtgacac cctgcggtgt ctgcctgcag gttctggccg aattctgcga
     1501 cgatctggaa atcatctcgg ccacgacttc cggcaaaaag gcaacctacc ggctgcgcga
     1561 actcttcccg aagccgtttc gccgctgatc ccggtcgtta cccgccacgg gagttcattt
     1621 gatcgaacaa gttctgctgg aaacctggcg cacagaccta tcccgaactc tggggattga
     1681 ttttccaccg ctttcccaaa attccggacg gtcggttgca ttttctcccg ccgagtttca
     1741 ttctctcccg gctcccgaaa aacggaagta cctgaccggc gtcagcgact acgccctcct
     1801 caagcccgat ctgacgtcgc cagtgctcga ggaggcttgt tcacgggccc gggaattggg
     1861 gtgctttggc ttctgcagct tgccctactt cgtcccgcag gcgcggaaat ttctggaggg
     1921 ctcgccggtg cacctcatct ccgtggttgg atttccctgg ggcgttcacc cgccgcagac
     1981 gaaacaaaaa gaaacggaat ggctggcggc cgaaggcgtg gctgaaattg atttcgtcac
     2041 caataccagt gccattaaaa aaggtgacta cgcgacagtc tgggaagaag tccggctgat
     2101 ccggcaaact ttccccttag gtatcctcaa gggaattatt gagtgtccgc ttttgaccac
     2161 cgaggagaaa gtcaaaaccg cctggctggt ggagcaagcc ggctgccagt tcgtcaagac
     2221 cggctccggc ctgttcggta aagtgcaggt ggcagacgta gaacttttgc gccggattct
     2281 tttgcccgca accaatatca aagccgctgg cggtattaga acactggagg atgcacttcc
     2341 tcttttagaa gccggcgccg aacggttggg aacttcgact ccggatacca tccttgctga
     2401 gattagcaac gcctaaccgg ttgttcctta accgatttca ccaattagca caaggggaat
     2461 attaggttgt tatgacccgt gaggtcatta ctttggtgga atgattcaac tccctctcct
     2521 gtagtggttc gatccgccat aggcggactc accaccctga gcctgtcgat ggggagaggg
     2581 ttagggtgag gtcgatatta cttaaacaac accattttct ttgtctccat aaaatcttct
     2641 gccttcaacc gatagaagta aatcccactg gccacttctt tgccccgatc atccgtcccg
     2701 tcccaggtta cttctttttc cccggcggag accacttcat tcactagcgt cttcaccctc
     2761 tgtcctaata gattgaaaat ctccaaggtc atattccccg gctggggtac tgcaaagcgg
     2821 atgatggtgc tgggattgaa ggggttcgga tgattctgct gtaaaaccca agaggaaggc
     2881 aacggtttgt caccattatc ttttaccttg gtaggagtag atatgttctt taagactgag
     2941 acggagtttc ctaaataatt cgccgtcact atgtcaatgt cgccgtcttt gtcgaagtcc
     3001 gaggctgaaa cagcagaggg ggcatttcca gttccgtaat ctgctctatt ggaaaaagaa
     3061 ccgtccccct tgtttttgaa gatcgaaaca gcattgccac tatttggggc aaccagatcg
     3121 ggatctccgt ctccatcaaa atcttggaca aaaatggaag aaaaataata cctcccataa
     3181 tccactttca ttgggaacgt cccatctcca ttgttcattg ataccgaaac ggtccatccg
     3241 gcagttatca agtcttggtc gccatctctg tctaaatctg aagcaaaaat tgcgtttggc
     3301 tcgatggctg agggatagtt gactggagag gcgaaagttc cgtctccgtt gttctttaat
     3361 accgacaaat aaccgacgtc caaagtattg tctgcggtta ccagatcttg atctccatca
     3421 ccgtcgatat cgcaagcaag aactgcgtaa ggccccgttc ctgtgcggta atcaatctta
     3481 ggagcaaatg taccgtctcc attattcata aagacagaaa cagtgttccc tctattcgca
     3541 acagccaaat ccggatcacc atcaccgtct aagtctgaag caaaaaccga gaatggagga
     3601 gtgctccctg ttccgtagtc aactttttca gcaaaagttc catcgcccag atttctcaag
     3661 accgaaactg tgtagctcca cgcattcgcc accgccaaat cttgacttcc gtcctcgtca
     3721 aagtctgaag caaaaattga gattggctta cttccggttc cgtaatccac tcttccagca
     3781 aacgtcccat ctccattgtt tttgagaacc gaaacagtac tactttcgga atttgctaca
     3841 accaaatctt ggtctccgtc ccgatctaaa tctgcggcca aaattgggga aggttcacct
     3901 cccgcggcat aattcgtctg gacaaaaaaa gtgccgtcgc ctttgttttt caataccgag
     3961 accgtgctac tgctaatact tgacgcgaca atatctgggc tcccattacc atccacatct
     4021 gcacaaaaga ttgaagaagg attccaaccc acaacgtagt ctcgtttggg tgcaaaagtt
     4081 ccgtccccgt tgttcaaaaa gagagagaca gtgttgctga gcatatttgc caccgctaaa
     4141 tccttatcac catccttatc taaatccgca acaaagactg atcgaggccg attccccgtt
     4201 tcatagtcga ctttgggagc gaaagttccg tcgccgttgt tcttgaagat tgagacgctc
     4261 tggctgctat cattggtcac tgctaaatcc tggtcaccat cgttatcgaa atctgaagcg
     4321 aaaaccgcag aaggagcatt tccggttgcg tagtcaatct tgagagcaaa agtgccatct
     4381 ccattgttct ttagaacgga aatacttcgg ctgaaataat ctgccgtaac caaatcggga
     4441 tggccgtctc catctaaatc cggagcgcag atggaagtag ggccgttccc cgttccgtaa
     4501 aaaaccctgg gagcgaatgt tccatcccca ttgtttttta ggaccgacac ggttttgccc
     4561 tgattggttg tgtgtcctgt attagctatt actaaatcta cgtccccgtc gtcgtctata
     4621 tcgacagcac tgatcgcttc gggtcgatat cctgcatcgt aatttactct aaccgcaaaa
     4681 gtgtcgtcac catagttcat taatactgct acagcattgc tgtcagaaat cgttactgcc
     4741 aaatcttggt ctccgtctct atctaagtct gaagcgacga ccgcgtaaat atagcgccca
     4801 atccggtaat ctgtcctagg agcaaaggtc ccatccccat tgttttttaa tactgaaaat
     4861 agaaagagac catcataact cggtatcacc aaatcttggt caccatcgcc atctaaatcc
     4921 gaggcaataa ctgccgaagg gtagacgcca acgtcgtagc taagccacgt atcaaacaat
     4981 ggctcaaaag ggaacaacgt ggcgggtgtc aagagaaatg cgagtgccaa aaggcacaag
     5041 aatcggctgg ccattttcct tcccctggca ataaatatat acgtatttac cgccgtcctt
     5101 tgactgcttt tcgcttttcc gcagtctttt tgtcctaaaa acaaaaagcc cccgctaaac
     5161 cgggggcttt ttggctgaag cgcactcttt acgggcagga gaaaggcagg ctgaagacca
     5221 cgttctggat catcgccacg atgtcgacaa ggtcaatgac accgtcgcag ttcacgtcaa
     5281 tggaggcctg accgttgacc ggacccggcg tgccgaagac cacgtacgcg acggtgccca
     5341 caatgtcggt gatgtccacg acgccgctgt tgtcaaaatc gcccaaaatg atcacggggt
     5401 agggtgcgtt gagccagttt aagacttttg tcatcacgtc ctgccgggtg gcaaaaccga
     5461 cgcgggtgct ggagataccc tcaaagccga aacccaggta aaccagttta taggtggtgt
     5521 ccttgatcac ccgcacgccg gcgggtttgc tgttggaaag gtaggtgaag atgctgtcgg
     5581 ctccgggctg cagtttcagc cagtccgccg aggtctggtt cgccgcacca tcggcgccga
     5641 ggatgtaaac gtccaacgcg ttggagatcg gatcgcccgt caccccattg gcccggatgg
     5701 tgaaggtatt cccgccgtag cggcagtgga ggtagttaat cagaaaactg gaatcggcgc
     5761 tggcggcgag cgcttcgccg atgctctgcc cggtgatgaa gagtttcccg cccccgtcga
     5821 gatacgcctt aagattggcc accgccgtgc tggtgagggt tgtcggcgtc cggttgttcc
     5881 cggtgaacca gatcacagtg gggtaggagg aaagagtcgg gatggaagga atcccgccgg
     5941 tgagcaccgt atgggagccg aacggtttgc gcacactgtc gagggtcgtc gtgtagaacg
     6001 attcccagtt gttggtcccg ccgtcgtcat ccacgagcaa aatcgtggtc gggcccaccg
     6061 tggctttgaa ggtttgctgc accgtcgcgc tgctgtcatt gttccaggcc aggacggtga
     6121 aaaagatcgt gtacgaattg ggcaagctgg ggtcggcggt aaactcgatc ggatcggaga
     6181 gcgaggtggt ggtcgccccc aggccgggca cgctggccag aagcgcggtg ctgtcggcaa
     6241 aaacgatccg aggatcgtcc gcccgcagga gcactgtcac gttggaggcg ttcgcccagg
     6301 cgtttttgag ccggacgtaa aagcgaaccg tctcggcaga atcgatcttg ccgttgttgt
     6361 tgccggtcgc ggcgtcgtcg aaccaggtgc tgtcgattga aaggtgcggc cggccgtagg
     6421 tcacctcgaa gttggcggtc atcgtgtcta agccgccgtt gccgaggtta aaaaccgcca
     6481 cctgagtggc ctcttggttg aacgggttct tgctgttggg gttggaaaat tcgtcaaaat
     6541 tccggttgtt gcttgtcccg gggaaaggat cgcccgcatc cccgcggttg ttgccgttct
     6601 ctaaattccg cagcccgtcg gcctgcagca ggtcaacttt cggcgggtta ttcccctggc
     6661 tctggctttc attgatttgc cagatcaaaa gcccctcgcc gttcagcgcc gcgtcaaagc
     6721 cggtgcgcct gcggttttcg acgtagaagt aggaggtgcc cagcgagccg ttcgtccaca
     6781 accgataagc tttattggtt cccacggtct tgggaatgga caccgcattc tgattggtcg
     6841 tgaccgtttc gatctccgcc caccggcgct ggaacttgca ccaggggtcc atatggcagg
     6901 gcgtcttgct gttgttcagc cagttgcagg agcccatcaa agaccattcc ccaacccctt
     6961 cgccgccggg attcgaatca acgtacaggt cggggaggaa gaagagcaaa tggcccgact
     7021 cgtggcagaa aacgccgatc gccaccatcc ggttgccgga attctcctcg ggacacacgg
     7081 tgtaacgcga gacggtcttc ccgtctaccg gcacggaagg agagatgttg gagacgtgcg
     7141 accacaaatc attcccgcta ccggtttcct cagccccaat accagcgtga acgacgtaga
     7201 agccatcgac tgttccgttc ccgtcattgt catagagggt gaagtccacg tccgggtcta
     7261 ctttgagtgc cacgtcccgc gctaattgtc gggcacgccc gttaggggcc aaacctgacg
     7321 ctccgttaca gtagtaggca tttgtgctat ccatccggta ccacccgatg acgtcgcctt
     7381 tgatgaacag tttcccgttg gtattctcca tatagaactc ggtgtaactg ccggtggggt
     7441 tcttgtggcc ggtggagaga agcagactgt cgaacatcgt cggagtggtg acggcgcctg
     7501 cgctggcggg cctatccggg aaatcaacca gaacgaagag cacacgtatt gtatcggtgg
     7561 ccgttatccc cgccagagtg ctggggcgca gccggtagcc cttactcgga tcagcgccct
     7621 ccgaagaaat gagcgcttcc aagtgctcaa cattcaaaag gcgctgcaat tcggcggtgg
     7681 cgccggcttt ccgcaacctt tcctttaccc ccggcgtggc cgacaccccg aaccttgggg
     7741 gggcggtgga aagaaaaagc gccgcagtta cgactccgac gaaggccaaa attccgtagc
     7801 gttgaaactg catctcaccc tccggttttg tggtactcat ttcccggtac tgttatgcgg
     7861 acaactcgtt ctgaaagcgt ctcatctgct ggtacagctc cgatgaaaag gtaatatata
     7921 ccatagcatt agactgtcaa tgagataaac cgcatccagc ttaagaattg ttcccatttt
     7981 tctcgtattt tcaggcaaac gccgtgccgg gccgcgcttt ttttatttac cagcgcaccg
     8041 cgctgcaacg caggttaagg aagtaaattc cgagtggagc catccggcgg gccaggccgg
     8101 gcgcaatcgg gcggaggagg tcggggaggg tggttccgag gccgatgacg aaggccctgg
     8161 tcacccggta gtccggaggc aggtggtcgg tctggtagcc cagcccgaca atcattattc
     8221 cccgcgcgag tttctccggg cgataggtaa tccagtacag aatgcggtcg aaatcgcggg
     8281 cattctgcga gaagaaatgg tggttttcgg cccaggggtt cccccgaaag gagattttga
     8341 catcaaaatt ctgcagcgcc tgcgacctgg ccttggccac cgccagcgca acaccggtgt
     8401 aatcgaacac gaggtcggat atacccagcc cctgctccgg gttgtaagca
//
LOCUS       DBNGZA010000240         9516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2271539, whole genome shotgun sequence.
ACCESSION   DBNGZA010000240 DBNGZA010000000
VERSION     DBNGZA010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2271539"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1060)
                     /gene="ispH"
                     /locus_tag="AC16FU_05000"
     CDS             complement(188..1060)
                     /gene="ispH"
                     /locus_tag="AC16FU_05000"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009442315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR2993764.1"
                     /translation="MEVILAKKAGFCWGVKRAIKIAAETAEKGEGPITALGPIIHNPQ
                     VISALRRKGVEIAKKLDDVEAGTVIIRAHGVKPEVKASAEGRGLKVVDATCPIVERNQ
                     EFARRLHQEGYLVVIIGEPNHPEVLGVMGYAEEDALVINANAADVEMNIPTNRRIGVV
                     AQTTLNVEAFKRIVGKIVEKAKEVKVYNTICNATDQIQKATVDLTEDVDLMIVVGGRE
                     SANTGRLKTMSEELDCPAYHIETAEELKSEWFVGINRVGVTAGTSTPDWIIQAVVERL
                     KSFDQSARQELTAS"
     gene            complement(1051..1758)
                     /locus_tag="AC16FU_05005"
     CDS             complement(1051..1758)
                     /locus_tag="AC16FU_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR2993765.1"
                     /translation="MNPYYKIGWRLSRFVGKLLWSFRAEGLEHVPPTGGMIIASNHRS
                     YADPPLVGAAILRQLFFFAKAELFTMPVLGWLVRHLNVLPIKRQRYDREGIERALEVL
                     RAGNGLVVFPEGTRCRTDDFLPPKSGIGWLAAEAKVPVVPAYIENSNHLWRALFLRKG
                     VTVRYGEPISAEWLARVPPGKEGYQMIVNEIMRRIRELKGQKQFAIPGGGSYISKLQM
                     EMKETPSRPMKAGEPWK"
     gene            complement(1755..2459)
                     /gene="cmk"
                     /locus_tag="AC16FU_05010"
     CDS             complement(1755..2459)
                     /gene="cmk"
                     /locus_tag="AC16FU_05010"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR2993766.1"
                     /translation="MPRHSRGKIIAIDGPAASGKSTTARLVAKKLGFLWLDTGAMYRA
                     VTLFALEQSVPGDNEPALAALARRLRFEFRAQHNGSRLWVNGRDVSQGIRSLFVTRAV
                     SQVSKHPQVRRVLVKQQQVCGRRGNIVVEGRDTTTVVFPRADLKIYLDASLKERARRR
                     LAELREAGVKSTLAEQMKAITLRDRSDSRRQASPLRRAPDAVYLDTTKLTVRQQVTAI
                     VRLFRQKFPGGTKRKI"
     gene            complement(2750..4762)
                     /locus_tag="AC16FU_05015"
     CDS             complement(2750..4762)
                     /locus_tag="AC16FU_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR2993767.1"
                     /translation="MPRNIPGKIIAIDDPAASGKNTIAPIQRKFTRSCLLPILVFLGT
                     LLLAACAPMHGGRGGYLNVIPVDQRPPLVGAGALADSLYGPAGESERGDGIAESRRAE
                     LEALVRRFAPTLILPDGDQATVNGRTYQLLPSDPQLFAETLQIDLICAAPYHFCDSLD
                     ILFRNLSTDSLAKLVEAAGRYESDPQVFAVWYFDFHGGNPREWWRTYGRLRTGPDSLL
                     WAQPTVYAHPFLDFLGRAVIQYWYFYAFNDFVGNHEGDWEHVNVVLTRGRDSVAEVHY
                     FFHARSMKLPQGAYRPRIVDQTHPVVYAGGRAYRLFDYSIQLFTGDLNAGSHGNYPYS
                     GRWEGAIALSQTEIVSPLGEDSSRVVPHDQFRVVLTPEPSRIDFRRKPEILKEWAWLM
                     LPVRWGFPSAPSVGAELKITDVGNRAPFGPAFNAGWNRTVHGFLYSPYAVRRIGVVRT
                     TINDLLQPWNFLYLYRYPRYFRTARYVHDTQSALNRRELSRRGLAPRGSWAERGLGTT
                     ILGAHLGIPAGDFSNVYRVSTGFSVWRNLWVKFRLGWLEVLAGYQKFLRKQKPRGSLY
                     IYPITGNVVLRLPDALFRPYLTLGGGWYWWQSQVSAPGDSLQYLASGRGLGTSVGVGV
                     EYYLKTKVALDVGLRYHSSPGPGAKAGLNSSRLRFLTLWIGHYFRF"
     gene            complement(5060..5710)
                     /gene="scpB"
                     /locus_tag="AC16FU_05020"
     CDS             complement(5060..5710)
                     /gene="scpB"
                     /locus_tag="AC16FU_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR2993768.1"
                     /translation="MEPKPTQIIEALLFAADHPLTAEEIAAALPEEASIPDLIDDLNR
                     FYETSGRAFRIREVAGGYQLATRPEFAPHVNSLFAGTRAQKLSRAGLETLSIVAYKQP
                     VIKAEIERIRGVQSDGVLKTLLERDLVAICGRQEGPGRPLLYGTTDKFLEYFGLKDLS
                     GLPTPDELGTLLSRQKLTELPATSPELELTLTSDASATNGDPVAADNAHAPESDTS"
     gene            complement(5682..6611)
                     /locus_tag="AC16FU_05025"
     CDS             complement(5682..6611)
                     /locus_tag="AC16FU_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR2993769.1"
                     /translation="MEPTLTYKKQDDSAPLLVKLDLFEGPLDLLLFLVQREEVPITDI
                     PIAKITQQYLDYLKAMETFNLEVAGEYIVMAATLIYIKTQLLLPRPQLEGEEGDPRAQ
                     LVQALVEYRRYKEAAVSLEKREETERQIFPHRDTSFLALKPEAPFELTATLFDLALAF
                     KEACERLPESALGGLEITYRRVTIEERIAHVLSVLKAKKRVPFWELYADEPVRPILIV
                     TFLALLELVRLQKLHLLQKDRTGEIWVMRFRAGERSDAVKEFPSEASESSAPVDRIKD
                     KRLISPVRKEPKRPVRKLIIKRGSDGAEAHPDH"
     gene            complement(6611..7612)
                     /gene="trpS"
                     /locus_tag="AC16FU_05030"
     CDS             complement(6611..7612)
                     /gene="trpS"
                     /locus_tag="AC16FU_05030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR2993770.1"
                     /translation="MSPARSGAGQSRPVVLSGMQPTGRLHLGNLEGALANWVKLQDEY
                     ECYFCIVDWHALTSDYDQTKDLKERIFQMTVDYLAAGLDPDKCAIFVQSQVKEHAELH
                     LLLSMITPVPWLQRVPSYKEKSEELKLDSYGFLGYPLLQAADILLYRANHVPVGQDQL
                     PHIELTREVVRRFNHLYGNVFPEPEALLTKFPVIPGIDGRKMSKSYNNDIAIADSPEI
                     TAKKISRMYTDPRKIYLGDPGHPEECPVYALHQIYNPQHETEIAPHCRSGVLACVDCK
                     KRMANYLNTALAPICERRLKLEKDKAAVMDVLKNGAEKARAKAQKTMEKVRKAIGLL"
     gene            complement(7609..8454)
                     /locus_tag="AC16FU_05035"
     CDS             complement(7609..8454)
                     /locus_tag="AC16FU_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993771.1"
                     /translation="MLTPSIVEGNVHESFSLPLEGRVREGSSFARKRRLVTRWTATVA
                     GLIFFTICKASHAQFPVIQGDFDISPLKTLRINYVLKGKLSGDQTLYIGGNRALVEIE
                     AGTAAPDNQKLSLKEYYSPEAKIVYDAARQVGRKADNVAYYRKQQIALLSKADRRYYE
                     KHRGQAQSSELSRLLGGTGAEKVSFLGKQCDVYRRPNLEVYLWKNIVVKMKVPALKID
                     MEATGIYENPKIPEDFLALPVGMPIAYDEKYSRAAKAGVDSLILAIKNRKLDQTPPRP
                     RSGSK"
     gene            complement(8611..9135)
                     /locus_tag="AC16FU_05040"
     CDS             complement(8611..9135)
                     /locus_tag="AC16FU_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR2993772.1"
                     /translation="MNADGGILRGNVLVLNGNYEPLSVCSVKRAVVMVFLGKAEVVER
                     HDGDSVHSVSVAIPVPSVVRLGFYIAVPQKRILLTRKNVIKRDGHRCQYCGEVRGPMT
                     VDHVIPKMYGGKDVWENLICACLRCNNIKGDRTPDQAGLSLLRQPKRPNHVTFIQQFI
                     GVSDLRWRPYLFLD"
BASE COUNT         1922 a         2786 c         2773 g         2035 t
ORIGIN      
        1 tcgaagcttg cccggtactg accagcgcaa aaaacaaaac gcccaccagc cccagcccgg
       61 cgagcgccaa agttcctttt ttcatttttc actaccgacc tcaccctgac cctctcctac
      121 gaggagaggg aaagtaaatc cctcccctgt agggtcggag actcgtaccg aggagggtgg
      181 ggtggggtca agaagcagtt aattcctgtc gtgctgactg atcaaacgac tttagtctct
      241 ccaccaccgc ctggataatc cagtccggtg tcgatgttcc cgcggtgaca cctacacgat
      301 tgatcccgac gaaccactcc gatttcagtt cctcggcagt ttcgatgtga taggccgggc
      361 agtccagttc ttcggacatc gtcttgagcc gtccggtatt ggcgctctcg cgccccccca
      421 caacgatcat caggtcgaca tcttcggtga ggtcaacggt cgctttctga atctggtcgg
      481 tggcgttgca gatggtgttg tagaccttca cttccttcgc tttctcgacg attttgccca
      541 cgatccgctt gaaggcctcg acgttcaaag tggtttgcgc caccacgccg atccggcggt
      601 tggtgggaat attcatttcg acgtcagccg cgttggcatt gataaccagc gcatcttctt
      661 cggcgtaccc catcacgccc agaacctccg ggtggttcgg ctcgccaatg ataacgacca
      721 agtacccttc ctggtgcaag cggcgggcga actcctggtt gcgctcgacg atcgggcagg
      781 tggcatcgac gacttttaat ccccggccct ccgcgctggc tttcacttcc ggcttgaccc
      841 cgtgtgcgcg gatgataacc gtgccggctt caacgtcatc taactttttg gcgatttcga
      901 cgcccttgcg ccgaagcgcg ctgatcacct gcgggttgtg gatgatgggc cccagagcgg
      961 tgatcggccc ttcccctttt tccgccgtct cggcggcgat tttaatggcc cgcttgacgc
     1021 cccagcaaaa acccgctttc ttggctaaaa ttacttccat ggctcacccg ctttcattgg
     1081 gcgcgaagga gtctctttca tctccatctg cagtttggaa atatatgagc caccgccagg
     1141 tatagcaaac tgcttttgtc cttttaactc ccggatacgc cgcatgattt cattgacgat
     1201 catctgatac ccctctttcc ccgggggcac gcgtgcaagc cactcagcag aaatcggctc
     1261 gccataacga accgttacac ctttccgcaa aaagagcgcg cgccagaggt gattggaatt
     1321 ttcaatgtag gccggcacca ccggcacctt cgcctccgcc gccagccacc cgatccccga
     1381 cttgggcggg agaaaatcgt cggtgcggca gcgggtgcct tcggggaaaa cgaccaggcc
     1441 gtttccggcc cggagcactt ccagcgcccg ctcaatcccc tcccggtcgt agcgctggcg
     1501 tttgattggc aaaacattca agtgccggac gagccagccc aaaacgggca tggtgaataa
     1561 ttcggccttg gcaaaaaaga acaactggcg gagaatggcc gcgcccacca ggggcgggtc
     1621 ggcgtaggag cggtggttgg aggcaatgat cattccgccc gtagggggaa catgttccag
     1681 cccctcggca cgaaaactcc agagtagttt tcccacaaag cggctgagcc gccagccgat
     1741 tttgtaatac gggttcatat cttgcgtttt gtcccgcctg gaaatttttg tcggaataat
     1801 ctgacgattg ccgtcacctg ctggcgcacc gtcagcttcg tcgtatcgag ataaacggca
     1861 tccggtgccc ggcggagtgg actggcttgg cgccggctgt cgctgcggtc acgcagagta
     1921 atcgctttca tttgctccgc cagagtgctt ttgacgcccg cctctcgcag ttcggccagt
     1981 cgtctgcggg ctcgctcttt gagcgaggca tcaaggtaga ttttcaaatc ggcccgcggg
     2041 aaaacgacgg tcgtcgtgtc gcgcccttcg acgacgatat tccctctgcg accacagacc
     2101 tgctgctgtt tcaccagaac cctgcgcacc tgcgggtgct tcgaaacctg ggacacggcg
     2161 cgcgtcacga aaagcgagcg gataccctgg ctgacgtccc gaccattcac ccaaagacga
     2221 gagccattgt gctgtgcgcg aaactcaaac cgcaaacgcc gcgccagtgc agccaaagcg
     2281 ggttcgttgt ccccagggac cgactgctcc agcgcgaaga gcgtgaccgc gcggtacatc
     2341 gcgccggtgt cgagccagag aaagcccaat tttttggcca ccagccgggc ggtggtgctt
     2401 ttccccgacg ccgccgggcc gtcaatggcg atgattttgc cgcgtgaatg tctgggcatc
     2461 ggatcaaaaa gataaacaga gatgcgttag cggccaacgg cgcaaaccgc aattggcgac
     2521 taaatttcaa atttccataa tggctgctta agtctatgac cggctcaggg acgagccggt
     2581 ttacctcgga tcggtagtcg ggctcgtctc agagcccgac ttcaaaactc aaaggtaagt
     2641 ctaaaaacct cggtacaaat cgttccgacc cgccaacgcc ggtcgggtct ttctcttttt
     2701 cgcgaaggcg gcacttcagt gccgccggtt gctgttcgac cccgacgtat cagaaccgaa
     2761 agtagtggcc gatccaaagg gtgaggaagc ggagccgact tgaattgagc ccggctttgg
     2821 cacccggtcc cggggaggag tggtagcgga gtcccacgtc cagtgcgacc ttcgttttca
     2881 agtaatattc aacgccgacg cccaccgacg tccccaggcc ccgtcccgaa gccaggtact
     2941 gcaggctgtc gccgggcgcg ctcacctgtg actgccacca gtaccagccg ccaccgagag
     3001 tgaggtaggg acgaaaaagt gcatcgggca gacgcagcac gacgttgccg gtgatcgggt
     3061 agatgtagag cgacccgcgc ggcttttgct tgcggagaaa tttctggtag ccagccagaa
     3121 cctcgagcca acccagacgg aatttgaccc agaggttgcg ccagactgaa aacccggtgg
     3181 agacgcggta gacgttggag aaatcccccg cgggaatgcc taaatgggcg cccagaattg
     3241 tggtccccaa tcccctctcc gcccatgagc cacgcggcgc caagccgcgg cgggagagtt
     3301 cccgccggtt gagcgcgctc tgggtgtcgt ggacgtaccg ggccgtgcga aagtagcggg
     3361 gatagcggta gaggtagaga aaattccagg gttgcaggag atcgttaatg gtggtccgca
     3421 ccacgccgat tcgcctcacc gcgtacggcg agtagagaaa cccgtgcacg gtgcggttcc
     3481 agcccgcatt gaaagccggg ccgaagggcg cccggttgcc cacatcggtg attttgagct
     3541 cggcaccgac ggagggtgcg ctggggaacc cccagcggac ggggagcatg agccaggccc
     3601 attctttgag aatttccggt ttgcgccgga agtcgatgcg ggagggttcc ggcgtcagga
     3661 cgacgcgaaa ttggtcgtgc ggcaccaccc ggctggagtc ctcgcccaaa ggcgaaacga
     3721 tctcggtctg actcaaggcg atggcacctt cccacctccc ggagtaggga tagttgccat
     3781 gtgacccggc attcaggtcg cccgtgaaga gctggatgga gtagtcgaag agccggtagg
     3841 ccctcccgcc ggcgtaaacc accggatgcg tctggtcgac gatccggggt cggtacgctc
     3901 cctgcggcag tttcatgctc cgggcgtgaa aaaagtaatg cacttccgca acactatcgc
     3961 ggccccgggt gagcactacg ttgacatgct cccaatcccc ttcgtggttg ccgacgaaat
     4021 cgttgaaggc ataaaagtac cagtactgga ttaccgctcg tccgaggaag tcgaggaagg
     4081 gatgggcgta gacggtgggc tgcgcccaga ggagactgtc cggaccggtg cgcagccggc
     4141 cgtaggtccg ccaccactcg cggggattcc cgccgtgaaa atcgaagtac cagaccgcaa
     4201 aaacctgggg atcgctctca tagcggcccg ccgcctccac cagcttggcc agcgagtcgg
     4261 tggatagatt ccggaagaga atgtctaagg aatcacagaa atggtacggt gcggcgcaaa
     4321 tcaagtctat ttgcaaggtt tcggcgaaaa gctgcggatc gctgggtaaa agctggtacg
     4381 tccgcccgtt gacggttgcc tgatccccgt cgggcagaat gagggtgggc gcgaaccgcc
     4441 gcacgagcgc ctccagttcg gctcgccgag actcggcgat tccgtccccg cgttcgctct
     4501 ccccggccgg tccatagagc gaatcggcca gcgcacccgc acccacgagc ggcggccgtt
     4561 gatcgaccgg aatgacattc aggtagcccc cgcgcccgcc gtgcatcgga gcacacgcgg
     4621 ctaagagcaa agttcccaga aaaaccaata tcggcagcaa gcacgaacgg gtgaactttc
     4681 tctggatggg agctatggtg tttttgccgg aggccgccgg gtcgtcgatg gcgatgattt
     4741 ttccgggaat atttctgggc atcagatcaa aaagataagt ggagatacgc tatcggccaa
     4801 cggcgcaaac cacaattagc gaccagattt caaatttccg ttacgggtgc tggaacctat
     4861 aaccggctca gggacgagcc ggtgtacctt ggatcggtag tcgggctcgt ctctgagccc
     4921 gacttcaaaa ccaaaaggtg gggctgaagc cccgcctacg cggtaaggta taacagcaga
     4981 cctagctttt cgcgttggca gcactgaagt gccgccgatt ggatggggtt tcggacgtaa
     5041 tcggcgcttg aaactgcagc tatgaggtat ctgactcggg cgcatgcgcg ttgtctgccg
     5101 ccaccggatc gccgttggtg gcggaggcat cagaggtcag cgtgagttcc agttccggag
     5161 acgtggccgg cagctcggtt aatttttggc gagacaaaag cgtgccgagt tcgtccgggg
     5221 taggtagacc ggagagatcc ttcaagccga agtattcgag gaatttgtcg gtggtgccgt
     5281 agaggagcgg ccggcccggg ccttcctgcc ggccacagat ggccaccaga tcgcgttcca
     5341 aaagcgtctt cagcacgccg tcggactgga cgccgcggat ccgctcgatt tcggccttga
     5401 ttaccggctg cttgtaggcg acaatcgaca gcgtctccaa cccggctcgg gagagtttct
     5461 gcgcccgcgt cccggcgaaa agagaattga cgtggggagc aaactccggg cgcgtggcca
     5521 actggtaacc gccggccacc tcccggatgc ggaacgcccg gccgctcgtc tcgtagaaac
     5581 ggttcaaatc gtcaatcaaa tcggggatcg acgcttcttc aggcagtgcc gcggctatct
     5641 cctctgcggt gagcggatgg tcggcggcga agagcagcgc ttcaatgatc tgggtgggct
     5701 tcggctccat ccgatcctct tttgatgatg agcttccgca ccggacgctt cggctctttg
     5761 cgcacgggag aaatcaaccg cttgtctttt atcctgtcaa ctggagcgga agactcggaa
     5821 gcttcggacg ggaattcttt tactgcatct gaacgctctc cggctcggaa gcgcatcacc
     5881 cagatttctc ccgtgcggtc tttctgcaaa aggtggagct tttgtaaccg caccaattcc
     5941 agaagcgcga ggaacgtgac aatcaggatc ggacgcaccg gttcgtccgc atacagctcc
     6001 cagaatggca cccgtttttt ggccttcaag acggaaagga catgggcaat gcgctcctcg
     6061 atggtgacgc gccggtaggt aatttccagt ccgcccaagg ccgattccgg cagcctttca
     6121 caggcttcct tgaaagccaa cgctaaatca aagagcgtgg cggtcaactc aaaaggggct
     6181 tccggtttga gtgcgaggaa actcgtgtcg cggtgcggaa aaatctgacg ttcggtttct
     6241 tcgcgcttct ccagtgaaac tgccgcctct ttgtagcgtc gatactctac gagcgcctgg
     6301 acgagctggg cgcgggggtc accttcctcg ccttccaact gcggccgcgg caggagcagc
     6361 tgggtcttga tatagattaa ggtcgccgcc atcacgatat attccccggc gacttccaga
     6421 ttgaaggttt ccatcgcctt gaggtagtcc aggtactgct gggtgatttt cgcgatcggg
     6481 atgtcggtga tggggacttc ctcccgctgg actaaaaaga gcaagaggtc cagcggccct
     6541 tcgaagagat cgagttttac cagaagcggc gctgagtcgt cttgcttttt gtaagtaagt
     6601 gtcggttcca ttacaaaagc cctatcgctt tgcgcacttt ttccattgtc ttctgggctt
     6661 tggcccgggc tttttcggct ccatttttga gcacgtccat cacggcggcc ttatcctttt
     6721 ccagcttcag ccgccgttcg caaattggcg ccaacgccgt attgaggtaa tttgccatcc
     6781 gcttcttaca atcaacgcag gccagaactc cggagcggca gtgcggcgca atttcggttt
     6841 cgtgctgggg attgtaaatc tggtggagcg cgtagaccgg gcactcctcc gggtggccgg
     6901 ggtcgcctaa ataaatcttg cgcggatcgg tgtacatccg cgaaatcttt ttggcggtta
     6961 tctccggcga atcggcgatg gcgatgtcgt tgttgtacga tttcgacatc ttccgtccgt
     7021 cgattcccgg gatcaccgga aacttcgtca ggagcgcctc cggttcgggg aaaacgttcc
     7081 cgtagaggtg attgaagcgc cgcaccacct cgcgggtcag ttcgatgtgc gggagttggt
     7141 cctgacccac cgggacgtgg ttggcacggt agaggagaat atccgccgct tgcaggagcg
     7201 ggtagcccag aaagccgtaa ctgtcaagtt tgagttcttc tgatttttct ttgtaggacg
     7261 gcacccgctg gagccaggga accggcgtga tcatcgagag gagcagatgc agttcggcgt
     7321 gctctttgac ctgcgactgg acgaaaatcg cgcacttgtc cggatcgagc cccgccgcca
     7381 ggtaatcgac cgtcatctgg aagatacgct ctttcaaatc tttggtctgg tcgtagtccg
     7441 aagtgagcgc atgccaatcg acaatgcaaa agtagcactc gtactcatcc tgcagtttca
     7501 cccagttcgc cagcgcccct tcgaggttgc ccaggtgcaa ccgcccggtg ggctgcatgc
     7561 cggagagtac gacgggtcgg gattgacccg caccggaccg ggccggactc acttggatcc
     7621 cgacctcggc ctgggtggcg tctggtcgag ttttcggttc ttgatggcta agatgagcga
     7681 atcgacgccc gctttggctg cgcgggagta tttttcgtcg taggcgatcg gcatccccac
     7741 gggcagcgcc aaaaaatctt ccgggatttt cgggttctcg taaatgccgg tggcttccat
     7801 gtcaattttt aatgccggga ctttcatctt cacgacgata tttttccaca gatagacttc
     7861 taaattgggt ctccggtaga cgtcgcactg tttgcccagg aaagaaactt tctccgcgcc
     7921 cgtgccgccc aaaagccgcg agagttcgga ggactgcgcc tgcccgcggt gcttctcgta
     7981 gtagcgccgg tcggccttgg agagcagggc gatctgctgc ttacggtagt atgccacgtt
     8041 atccgccttg cgccccacct ggcgcgcggc atcgtagacg atcttcgctt ccggcgagta
     8101 gtattccttc agcgacaact tctgattgtc tggcgcggcg gttccggctt cgatttcgac
     8161 cagggcccgg ttgccgccga tgtagagcgt ctggtcgccg gagagtttac ctttcaacac
     8221 gtaattgatg cgcagcgttt tgagcgggga gatgtcgaaa tcgccctgga tgacggggaa
     8281 ctgggcgtgg gatgccttgc agatcgtaaa aaaaattagc ccggcgaccg ttgctgtcca
     8341 cctcgttacg agtcttcttt ttctggcaaa agatgacccc tccctcaccc tcccctccaa
     8401 ggggagggaa aaactttcat gcacatttcc ttcaacaatt gaaggggtta acattccctc
     8461 tcctcctagt ggttcgacct tgctcaccac cctgagcctg tcgaagggga gagggttggg
     8521 gtgaggtcaa aagaagaaaa ccgcaacgcg ggtcgtgtgc ctggttccaa cttttccagc
     8581 cggtgcctcc tcagttcgcg tcgtcctccg ctaatctaaa aacagatacg gccgccagcg
     8641 caggtccgaa acgccgatga actgctggat gaaagtgacg tggttgggcc gcttgggctg
     8701 gcgcaaaagc gaaagccccg cttgatccgg cgtccggtcg cctttgatgt tgttgcagcg
     8761 caggcaggcg cagatcaaat tctcccacac gtctttgccg ccgtacattt tgggaatcac
     8821 gtgatccacc gtcatcggcc cgcgcacctc gccgcagtac tggcagcggt gtccgtcgcg
     8881 cttgatgacg ttcttgcggg tcaaaaggat ccgtttctgc ggcaccgcga tgtagaatcc
     8941 caggcgcacg acggaaggaa cggggatcgc cacggaaacc gaatgcaccg agtcgccgtc
     9001 gtgccgctcc accacctcgg ctttgcccaa aaagaccatc accaccgccc gcttgaccga
     9061 gcagaccgag agcggctcgt agttcccgtt caaaacgagc acgttgccgc gtaatatgcc
     9121 accgtccgcg ttcatcgcca atcagttcgc tcttggtaat ttatacgcct ctctccgtct
     9181 gtcaattgcc tccgcaacag tttcggtttc ttgtaattca ccgcctgaag ggaaactcaa
     9241 tcttttccgg gaacgaaacc ctcctcccct gcgtatccca tatgaaagaa aactgttgac
     9301 tttttgcgta aatgccttat tataaaactg cccgctcggt acgagtctat tgcgaccgga
     9361 agcggaattg acaggtgata ggaccccgaa aaaggataaa ttccatagga cggtccgcct
     9421 caggcagatc gattttggac gtttctgaaa ggagattgga gacgctatga acttcgaccg
     9481 ccgttcccaa atagtcgcgc tgattctggc catcgt
//
LOCUS       DBNGZA010000241         8909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2588308, whole genome shotgun sequence.
ACCESSION   DBNGZA010000241 DBNGZA010000000
VERSION     DBNGZA010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2588308"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="AC16FU_05045"
     CDS             complement(<1..363)
                     /locus_tag="AC16FU_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2993773.1"
                     /translation="MKVIIPVAGGGTRLWPLTQRTPKVLLQVADRPMLGHILKQVEKV
                     EPEEVIFIIGAGGDAIVNYVVRTFDFPVRFVEQTDLIGLGYAVALASPFFGRGPLLVI
                     LGDTLIGADLKKIMNIGHD"
     gene            complement(503..1588)
                     /locus_tag="AC16FU_05050"
     CDS             complement(503..1588)
                     /locus_tag="AC16FU_05050"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR2993774.1"
                     /translation="MSKSKNRGDDVAGLDDPQVYRTLDPENYYRLIYDFPLQLKEALA
                     LSFSAAPEQPFDSIVLCGMGGSAIAGDLVLSLLRYELSLPFVVVRQYHLPKSVTALGL
                     VIASSYSGNTEETLAAYDQARARGCPIVALTTGGELLLRAERDGCGYCVLPPGYPPRA
                     ALAFSFVPLLGLLTQLRLTHDWREEVQKASEFLRHVREEWNQKSPVAKNLAKQIAEKL
                     YRRIPIIYASGDRLDAVATRWKGQICENAKTLAFANFFPEFNHNELVGWQVLEGVREK
                     LIALFLEDAGDHPRIRRRSEIVQRVIEGKNVAALTIPSQGTSFLSRLLYLVYLGDLAS
                     YYLALLSGVNPKPVAVIDFLKAELVRS"
     gene            complement(1542..3284)
                     /gene="ptsP"
                     /locus_tag="AC16FU_05055"
     CDS             complement(1542..3284)
                     /gene="ptsP"
                     /locus_tag="AC16FU_05055"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR2993775.1"
                     /translation="MKVYRGIPAAAGMAIGRATVWGTPEPSAVSRTIRSADVAGEKAR
                     LARAVERAQAELRRMAQKLPKTDPGVKLLLAQQMMVTDPEILKETEAVISSHFVDAAT
                     AYRQAVERVRSSIAATANTYLRDRANDLAGASSLVLKYLSGGERLPRRVKPGQIVLAC
                     NLSPGDVLTLCRQQVRGLVSETGGATSHAAILAKAWGIPAVMGLVNVTKKLGGKGSPV
                     IVDGGTGKVIINPDSKTREEFERRSKREKREQRALLALRRLPAVTRDGHRVRLSANLD
                     FPEETEAARRNGAEGVGLFRTEYLLTNQGIPTEEEQSAVYRKIVRTFAPHPVTLRTFD
                     VGGDKFRRGALPEANPFLGWRAIRIGLDEAAFLATQLRAMLRASTYGRVRILLPMVAT
                     VAEVTAAQKIFAQAKQALQHRNVRFDPETLLGIMIEIPAAALVAEHLAAQVDFFSIGT
                     NDLAQYTLAVDRGNPKVAHLFDPFDPSVLRLLAMSLAATKKQGIEVSVCGEMAADPLA
                     VPLLIGLGVQELSVVPALLPGVKRLIRELRFADLGALASEVLEQASPGAVKAILRRFL
                     EQIEKQGRRRGRLG"
     gene            complement(3293..3559)
                     /locus_tag="AC16FU_05060"
     CDS             complement(3293..3559)
                     /locus_tag="AC16FU_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR2993776.1"
                     /translation="MIEKTVMVTNRLGIHARPAAMLVQKASKFKSEIRLVKGALEVNA
                     KSIMGVMMLAAECGSKLFLRAWGPDAEQAVAELTDIFESKFNEE"
     gene            complement(3556..4686)
                     /gene="dprA"
                     /locus_tag="AC16FU_05065"
     CDS             complement(3556..4686)
                     /gene="dprA"
                     /locus_tag="AC16FU_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR2993777.1"
                     /translation="MALTDAEKENWLALSLVPNVGPLHFTALVKRFGSAGAVLEAGEK
                     SLLEVPGVGLKTAAAIRRTPVREEARKQLALLQKEKCDLITLADESYPFNLKNISDPP
                     PVLFVRGKLLPQDKQAVAIVGARSQTHYGESLTENLAGALAQNGVTVVSGFARGIDAV
                     AHRAALKSGGRTIAVLGCGLDVLYPPEHRELREAIAAKGAVISEFPFGTPPDPFNFPR
                     RNRIISGLSLGVVVVEAGSKSGALITAEHALEQGRDVFAIPGNVFAKNSQGTNKLIKQ
                     GAALVTSVEDVLSQLQGLARTQKSRSSTAPLLEPLGLSEEEQHLLRLLSEEPVHIDLL
                     TQKAALSVGQVLAALTNLEFKGVVRQLAGKQFVRQMAPELVP"
     gene            complement(4689..5672)
                     /gene="obgE"
                     /locus_tag="AC16FU_05070"
     CDS             complement(4689..5672)
                     /gene="obgE"
                     /locus_tag="AC16FU_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR2993778.1"
                     /translation="MFVDYVEITVKAGDGGHGCLSFRREKYVPKGGPDGGDGGRGGHV
                     IARVDPHLATLLDLRYQRLYRAAKGGDGQGKDLFGREGEDRVIPVPPGTTIKDLNTGK
                     VIADLTLPDQEVLLARGGRGGHGNAFFKSSTNRAPRRFEAGKKGEEKRLTFELKLIAD
                     VGLVGFPNVGKSTLLAKVSRARPKIAPYPFTTLTPHLGIVRVGDWRSFVLADIPGIIA
                     GAHQGKGLGLEFLRHIQRTRVLVYLLDATSSTLRRDYQILKAEVKAYDPEITRKPSLV
                     VLNKIDLLNGQPKKTLRVSMPVFYISALTGQGIKELLFGLEEVLGSVSRAA"
     gene            complement(5739..6503)
                     /gene="cdaA"
                     /locus_tag="AC16FU_05075"
     CDS             complement(5739..6503)
                     /gene="cdaA"
                     /locus_tag="AC16FU_05075"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR2993779.1"
                     /translation="MNFFTFGFLKFGLVDAVDIGLVSFVTYKLLSLMKGTRSAQILTG
                     LFLVIAVSFLAFWFQLEAVKWLITNIATVGLLVLVVLFQPEIRGALAQIGHNRVVRLF
                     YKYEEQKSLDEIVRATQKLAESKTGSLIVLERQVGLKSYIESGRLMHAQVSEELLITI
                     FSPRAPLHDGAVIIRGDLVVAAGCTLPLSRTLEFTAPHGMRHKAATGITEESDAIAVA
                     VSEETGEISVAGWGRLERGIDPHALRDHLVRLLRSQ"
     gene            complement(6540..7424)
                     /gene="folP"
                     /locus_tag="AC16FU_05080"
     CDS             complement(6540..7424)
                     /gene="folP"
                     /locus_tag="AC16FU_05080"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR2993780.1"
                     /translation="MKVSPENAAPKKKSKRGVIRCGEQEINLFSRTHIMGVLNVTPDS
                     FYDGGRYYDLRRAVNRAREMTDEGADFIDIGGLSTRPGSEPVSEEEEIKRVIPVVTEV
                     ATQVSIPLSIDTYRASVARAALDAGASLVNDISGLAFDPAMPKLVAESGCPVVIMHIK
                     GTPRDMQVDPHYDDVIAEISRHFRDRIASAKSAGIADEQILLDPGIGFGKRMEDNLVI
                     LQRLRDFADLGYPLLVGASRKSFIGKILDLPSAERLEGSLAAAAVAIQNGAAIIRTHD
                     VKATVRVARIVDAIRRSG"
     gene            complement(7459..7953)
                     /locus_tag="AC16FU_05085"
     CDS             complement(7459..7953)
                     /locus_tag="AC16FU_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993781.1"
                     /translation="MTCRFDNSDTLYLCSAYKLPRPTEITDIYELVITKDSTNGKIVF
                     SRNYYPESPDSESQQDNILGLTVEYNGSYNYLVVVGTVGNGVLTRFLAKKNGRFQQLA
                     EFERVRVEFADVDNDETDEVLVPEDKFSPDLGVFPDVNIYKWQGWELKWPRTVPYKKR
                     LCEN"
     gene            complement(8088..8648)
                     /gene="folE"
                     /locus_tag="AC16FU_05090"
     CDS             complement(8088..8648)
                     /gene="folE"
                     /locus_tag="AC16FU_05090"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR2993782.1"
                     /translation="MDKKRIATAVRLLIQAIGEDPERDGLKQTPQRVADFFEEIFSGV
                     GRDPKEALKLYSTPNQDEMIILKDIPFYSLCEHHLLPFFGKAHIAYIPNENKITGFSS
                     LARVVEASAHRLQLQERLATEIADVIMEVVQPKGVLVVIEAEQLCLTMRGIKKPGSLA
                     VTSAIRGAMHNAPTRAEAFALIKGKA"
     gene            complement(8641..>8909)
                     /locus_tag="AC16FU_05095"
     CDS             complement(8641..>8909)
                     /locus_tag="AC16FU_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993783.1"
                     /translation="AKHRDYSENTAQEIDKEVKKIVEEAERRATDLLSVNLEKLHLLA
                     KALLDREILDGEEIDRLLRGEELLPHPDGAKPTKPEAVALKSDG"
BASE COUNT         1840 a         2692 c         2501 g         1876 t
ORIGIN      
        1 atcgtggccg atgttcatga tttttttcaa atccgcgccg atgagggtat cgccgagaat
       61 gacgagaagc ggcccgcggc cgaaaaaagg actcgccaga gccaccgcgt accccaggcc
      121 gatcaaatcg gtctgctcca cgaagcgcac cggaaaatcg aaggtgcgca ccacgtagtt
      181 gacgatggcg tcgccgccgg cgccgataat gaagatcact tcttcgggtt ccaccttttc
      241 aacctgcttc aagatatgtc ccagcatcgg ccggtcggct acctgcagga gcaccttggg
      301 cgttctctgc gttaacggcc agagccgcgt tccgcctccg gccaccggaa taatcacctt
      361 cataatagat caactgcctc ttcgccttcc agttggataa ctgttcggga agcgcggaca
      421 atttactctt tgtaagagaa ttacgcaagc cgagctacag agacgacgtg ctcaagcaca
      481 ttcccgctgc ttttaccgtc gctcaagacc ggaccaattc agctttgagg aaatcaatta
      541 ccgcgaccgg tttgggattg accccgctga gcagggccag atagtaactg gccaaatcgc
      601 ccaggtaaac gaggtacagc aggcgggaga gaaagctcgt gccctggctg ggaatggtga
      661 gggctgcaac gtttttccct tcgatcaccc gctggacaat ctcgctgcgc cggcggatgc
      721 ggggatggtc gcctgcatct tcgagaaaca gagcgatgag cttctcccgc actccctcca
      781 gcacctgcca gccgaccagt tcattgtggt tgaactccgg gaagaaattg gcaaaggcca
      841 gtgtcttggc attttcacaa atctgccctt tccagcgggt cgccacggcg tccaggcggt
      901 cgccgctcgc gtagatgatt ggaattcttc gatagagttt ttcggcaatc tgcttcgcaa
      961 gattcttggc aaccggtgac ttttgattcc actcttcacg gacgtgcctg aggaattcag
     1021 aagctttttg cacctcctcg cgccagtcgt gggttaacct gagttgggtc agaagtccca
     1081 gaagcggaac gaacgaaaac gccagtgccg cccggggcgg gtagcccggc ggcagcacgc
     1141 aatagccgca accgtcgcgc tcggcccgca agagcagttc accgccggtg gtaagcgcga
     1201 cgatcggaca gccgcgcgcc cgggcttgat cgtacgccgc aagggtctcc tccgtgttgc
     1261 cggaatagct cgacgcaatc accagcccaa gggcagtaac acttttcggg aggtgatatt
     1321 gacgtacaac gacaaaagga agcgaaagtt cgtagcgcag gagcgagagg accagatcgc
     1381 cggctatggc cgaccctccc atcccgcaga gcacgatgga gtcgaatggc tgctcgggag
     1441 ccgccgaaaa agaaagcgcc agggcttctt tcaactgcag cgggaaatca taaatcagcc
     1501 ggtagtaatt ttccgggtca agcgtccggt aaacttgggg gtcatccaag ccggccacgt
     1561 cgtctcccct gtttttcgat ttgctcaaga aagcgccgca gaattgcttt aaccgccccc
     1621 ggactcgcct gttccagaac ctcggatgcc aaagcgccta aatccgcaaa ccggagttcc
     1681 cgaatcaacc gcttgacccc gggcaagagc gccgggacca ctgagagttc ctgcaccccc
     1741 agaccgatca ataagggcac tgccaaaggg tcagccgcca tctcaccgca caccgacacc
     1801 tcgataccct gcttttttgt tgcggcgagg gacatggcca aaagccgcag cactgacgga
     1861 tcgaacggat cgaacaggtg tgccactttg ggatttcccc ggtctaccgc taaggtatac
     1921 tgcgccaggt cgttggtccc gatgctgaag aaatcaacct gcgccgcgag gtgctcggcc
     1981 accagtgccg ccgccggaat ttcgatcatg attcccagaa gcgtctctgg atcgaaacgc
     2041 acgttccggt gctggagtgc ttgtttcgct tgtgcaaaaa ttttttgggc agccgtaact
     2101 tcagcaaccg tggctaccat cgggagcaga atccgcaccc gaccgtaggt cgaagcgcgg
     2161 agcatggccc gcagttgcgt ggccaggaaa gccgcttcgt ccagcccgat ccggatcgcc
     2221 cgccagccca gaaagggatt ggcttccggc aatgctcctc tgcgaaactt atcaccgccc
     2281 acgtcgaagg tccgcagcgt caccggatgc ggagcaaagg tgcggactat ttttcggtaa
     2341 acggccgact gctcctcttc ggtcggaatc ccctgattgg tcaacaggta ctcggtacgg
     2401 aacaggccca ccccctcggc tccgttccgg cgggccgcct cggtctcttc agggaaatcg
     2461 aggttggccg atagccgcac ccggtgacca tcgcgggtca ctgcggggag tcggcgaagc
     2521 gccaaaagcg cgcgctgttc tcgtttctcc cgtttactgc gccgctcgaa ctcttcgcgc
     2581 gtttttgaat cgggattgat aatgacctta ccggttcctc catccacaat gacgggggaa
     2641 cccttccccc caagtttctt agtaacgttg acgaggccca tcaccgccgg aatgccccag
     2701 gctttggcca gaatcgccgc gtgggaggtg gccccgccgg tttcggaaac caacccgcgc
     2761 acctgctgac gacagagcgt caggacatcc ccgggcgaaa gattgcaggc caagacgatc
     2821 tgccccggtt ttacccgccg gggaagacgc tccccacccg aaaggtactt gagcaccaaa
     2881 gacgaagccc cggccaagtc attggcgcgg tcgcgcaagt aggtattcgc ggttgcggcg
     2941 attgaactgc gcacccgttc aacagcttgg cggtaggcgg tggcggcatc gacgaaatgt
     3001 gagctgatga ctgcctcggt ctctttgaga atttccggat cagtcaccat catttgctgc
     3061 gccaggagaa gttttacccc gggatcggtc ttcgggagtt tctgggccat ccggcgcagt
     3121 tcggcttggg cccgctctac cgctctggcg agccgtgcct tttccccggc gacgtcagcg
     3181 gacctgatcg tgcgggagac cgccgagggc tccggtgtgc cccaaacggt tgcccgaccg
     3241 atggccatcc ccgccgccgc cggaatcccc cggtatacct tcatttttcc gctcattctt
     3301 cgttgaactt actttcaaaa atatctgtca gttcagccac cgcctgttcg gcatcgggac
     3361 cccaggcgcg gaggaaaagt ttgctgccgc actcggctgc gagcatcatc acccccatga
     3421 tgcttttggc gtttacttcg agcgctccct ttaccagccg gatttcactc ttgaatttgc
     3481 tcgccttctg caccagcatc gccgccgggc gggcgtgaat gcccagacgg ttggtcacca
     3541 tcaccgtttt ttcgatcacg ggaccagttc cggtgccatc tgccgcacga actgcttccc
     3601 cgccagctgc cgcaccaccc ccttgaactc cagattcgtc agggccgcca ggacctgccc
     3661 caccgagagc gccgccttct gtgttaaaag gtcgatatgc accggttcct ccgaaaggag
     3721 ccgcagcaag tgctgttcct cttccgaaag accaagcggc tcgagtagcg gcgcggtgct
     3781 gctccgggat ttctgcgtcc gggccagccc ctgcagctgc gacagcacgt cctctaccga
     3841 cgtgaccagc gcggctcctt gcttgatcaa tttgttcgtc ccctgactgt ttttggcgaa
     3901 gacattcccg gggatggcga agacgtcgcg tccctgctct aaggcgtgtt cggcggtaat
     3961 gagcgcgccc gatttcgatc cggcctccac gacgacgacg cccaaggaca gcccgctgat
     4021 gatccggttg cgccggggaa aattgaaagg atcaggcggt gtgccaaagg gaaactcaga
     4081 aatgaccgcg cctttagcgg cgattgcttc gcgcaattct ctgtgctcgg gcgggtagag
     4141 cacatccaac ccgcagccca ggacggcgat cgttcgcccg cccgatttca aggccgcccg
     4201 gtgcgccacg gcatcaatcc cccgcgcaaa accactcacc accgtgacgc cattctgggc
     4261 cagcgccccg gccagatttt cggtcaagga ctccccgtag tgggtctggg agcgggcgcc
     4321 gacgatggcc acggcctgct tatcctgcgg caaaagcttt ccccggacga agagcaccgg
     4381 cggcggatcg gagatatttt tgagattgaa cgggtagctc tcatcggcta acgtgatgag
     4441 gtcacacttt tctttctgca aaagagccag ttgcttccgg gcttcctccc gcaccggcgt
     4501 gcgccgtatc gctgcggcgg tctttaaccc gacgcccgga acttcgagga gtgacttctc
     4561 gccggcctcc agaacggcac cggcagagcc aaaacgtttc accagcgcgg tgaaatgcaa
     4621 aggcccgaca ttcggcacga gcgaaagcgc cagccagttt tctttttccg cgtccgtcag
     4681 cgccatactc aggccgcccg ggaaactgaa cccaggacct cctccaggcc gaaaagcaac
     4741 tccttgattc cttggccggt caaagccgag atgtaaaaaa ccggcatcga cacccgcaac
     4801 gtcttcttgg gctggccgtt gaggagatcg atcttgttta gcaccacgag cgacggcttc
     4861 cgggtgattt ccggatcgta ggccttcact tccgccttta agatttggta atcgcggcgg
     4921 agcgtggaac tggtggcatc cagaaggtaa actaaaacgc gcgtccgctg aatgtggcgc
     4981 aaaaattcca gccccagccc tttgccctga tgtgccccgg cgatgatccc gggaatgtcg
     5041 gccaaaacga atgagcgcca atcgccaaca cgcacgatgc ctaagtgcgg cgtaagcgtg
     5101 gtgaaaggat agggcgcgat tttcggccgg gctctggaga ctttcgctaa gagggtcgat
     5161 ttgcccacat tcgggaaacc gaccaagccg acatcggcga tcagtttcaa ttcaaaagtc
     5221 aggcgcttct cttctccttt tttacccgcc tcaaagcggc gcggcgcccg gttggtggag
     5281 gatttgaaaa aggcgttccc gtgcccgccc cggccgcctc tggccaaaag cacttcctga
     5341 tccggaaggg tcaaatcggc aatgactttt ccggtgttga ggtctttgat ggtcgtcccc
     5401 ggcggcaccg gaatgacccg gtcttcccct tccctgccga acaaatcttt cccctgaccg
     5461 tcgcccccct tggccgcccg gtagagtctc tggtagcgca aatctaaaag tgtggccagg
     5521 tgtggatcaa cccgggcgat cacgtgcccg ccccgcccgc cgtcccctcc gtccgggccg
     5581 cctttgggca catacttctc gcgccggaac gagagacacc cgtgtccgcc gtcacccgcc
     5641 ttgactgtga tttcaacgta gtcaacgaac ataactctgt tatgcgcaaa ccatatggcc
     5701 gcaatgaatc gctctcggca cccgtcggcg cgagcacctt actggctccg caggaggcgc
     5761 accaggtggt cccggagcgc atggggatcg attccccgct ccagccgccc ccagccggcc
     5821 accgaaattt cgccggtttc ctcggatacg gcgacggcaa tggcatccga ttcttcggtg
     5881 atgccggtgg cagctttgtg ccgcatcccg tgcggggcgg tgaactccag cgtccgcgaa
     5941 aggggcaggg tgcagccggc cgccaccact aagtcgcccc ggatgatgac cgcgccgtcg
     6001 tgcaacggcg cccgcggcga gaagatggtt atcaaaagtt cttcggacac ctgcgcgtgc
     6061 atcaggcgcc ccgattcgat gtacgatttc aaccctacct gccgctccag aacgatcaag
     6121 ctgccggtct tggactcggc cagcttctgc gtggcccgca cgatttcatc caacgatttc
     6181 tgctcctcat acttgtagaa gagccgtacc acccggttgt ggccgatctg cgccaacgcc
     6241 ccgcggattt ccggttggaa gaggacgacc aataccagaa gccctacggt ggcgatgttc
     6301 gtgatgagcc acttcaccgc ttccaactgg aaccagaacg ccagaaacga cacggcgatc
     6361 acgagaaata ggccggtcaa aatctgggct gagcgcgtcc ccttcatgag cgagaggagt
     6421 ttataggtca caaaactgac gaggcctatg tccacggcat cgaccaggcc gaatttcaaa
     6481 aagccgaacg tgaaaaagtt cacgcaaaaa gtatagggag atttacggag agatgcaact
     6541 caacctgacc ggcggattgc atcgacgatc cgtgccaccc gcacggtggc tttaacatcg
     6601 tgggtgcgga tgatggcggc gccgttctgg atggccaccg ccgcggcggc caagctccct
     6661 tccagacgtt ctgcagaggg gaggtctaaa attttgccga tgaaactctt gcgcgacgcg
     6721 cccaccaaaa gcgggtagcc caagtccgcg aagtcgcgca gtcgttggag aataaccagg
     6781 ttatcttcca ttcttttgcc gaagccgatg ccggggtcta agagaatctg ttcgtcagca
     6841 atgccggctg actttgcgga ggcgatgcgg tcccggaaat gccggctgat ttcggcaatg
     6901 acgtcgtcgt agtgcggatc gacctgcatg tcgcgcggcg tgcccttgat gtgcatgatg
     6961 acgaccggac agccgctttc cgctacgagt ttcggcatcg ccggatcaaa agccagcccg
     7021 ctgatgtcgt tcaccagaga ggcgccagca tccagagcgg cccgggccac gctggcgcgg
     7081 taagtatcaa tcgaaagggg gattgacacc tgcgtcgcga cctcggttac gacgggaatc
     7141 acccgcttga tttcttcttc ttcggaaacc ggctcggagc cgggccgggt cgaaagcccg
     7201 ccgatgtcga tgaaatcggc cccttcgtcg gtcatttcgc gcgcgcggtt gactgcccgc
     7261 cttaggtcgt agtaccggcc gccgtcgtag aaggagtcgg gcgtgacgtt gagcacaccc
     7321 ataatgtgcg tgcgggagaa gagattaatc tcttgctcgc cgcagcggat gacaccgcgc
     7381 ttcgatttct tttttggagc tgcattctct ggggaaactt tcattcgtac tgagtaatca
     7441 attggtgtga agtggtcttt aattttcaca taatcttttc ttatagggaa ccgtacgcgg
     7501 ccatttcaac tcccatccct gccacttgta aatattaacg tctgggaaca ctccaagatc
     7561 tgggctgaat ttgtcttcag gtacaagaac ttcatccgtc tcatcattat cgacatctgc
     7621 aaattctact ctgacccgtt caaactcagc caactgctga aatctaccat ttttcttagc
     7681 aaggaatcgg gttaacaccc cattgcctac ggtgccaaca accacaaggt agttatacga
     7741 tccattgtac tcaaccgtca aaccaagtat attatcctgt tgactttcac tatcaggtga
     7801 ttctgggtag taatttctcg agaagacgat ttttccatta gtactatctt tcgttatgac
     7861 gagttcgtat atgtctgtaa tttctgtggg acgggggagc ttgtatgcgg agcaaaggta
     7921 cagtgtatct gaattatcaa acctgcaggt cactacggcg ataagacgtt taagacttct
     7981 tgttggcact ttttcaactt gcttactgga ttgacaggag aaaagaacca agattgaaaa
     8041 cagagggaaa agaaatgaat ggtgctttcg gaacaaggta ttaaaagtta cgctttccct
     8101 ttaatcagcg caaacgcttc ggcgcgggtg ggggcgttgt gcatcgcccc gcgaatggcc
     8161 gaggtgacag ccagagaccc cggcttttta atcccccgca tagtgaggca gagctgctcg
     8221 gcttcgatga cgaccaaaac ccccttgggc tgtactactt ccatgatgac gtcggcgatt
     8281 tcggtcgcca gccgctcctg caactggagc cggtgggcgg aggcctccac cacacgggcc
     8341 aaactggaga aaccggtgat cttgttctca ttgggaatgt aggcgatgtg cgccttgccg
     8401 aagaagggca acaggtggtg ctcgcagagg gagtagaagg gaatgtcctt caggatgatc
     8461 atctcatcct ggttgggcgt ggagtagagc ttgagcgctt ctttggggtc gcgcccgacg
     8521 ccggagaaaa tctcttcaaa aaaatcggcg acccgctggg gtgtttgctt tagaccgtcg
     8581 cgctccgggt cttcgccgat cgcttggatc aaaagccgca ccgcagtggc aattctcttt
     8641 ttatccatcc gattttaagg cgaccgcttc cggcttggtc ggcttggccc cgtcggggtg
     8701 cggcaaaagt tcctccccgc gcaggagccg gtcgatttcc tcgccgtcta aaatctcgcg
     8761 gtccaaaagg gccttggcca acaggtgcag tttttccaga ttgaccgaga ggaggtcggt
     8821 ggcgcggcgc tcggcttctt ccacgatctt tttcacttcc ttgtcgattt cctgcgccgt
     8881 gttttcgctg tagtcccggt gcttggcga
//
LOCUS       DBNGZA010000242         6646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_998573, whole genome shotgun sequence.
ACCESSION   DBNGZA010000242 DBNGZA010000000
VERSION     DBNGZA010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_998573"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..795)
                     /locus_tag="AC16FU_05100"
     CDS             complement(67..795)
                     /locus_tag="AC16FU_05100"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR2993784.1"
                     /translation="MPKLSVNVDHVATLREARKIKEPDPVFAAMLAELAGADGITVHL
                     REDRRHIRDRDLYVLKEVVKTELNLEMAAIPQMVERALEVKPHLVTLVPEKREELTTE
                     GGLDVTGFSERIGAVLKQMKPTGIRVSLFIDPEVEQIKAAAKLRAEIIELSTARYAES
                     KTDKEIDEELKKIDQMTDMADKLGIEVHAGHGLNYRNIVPLLEIEKITEYAIGHAIVA
                     RAVLVGFSQAVKEMIQIIRERCPN"
     gene            complement(804..1319)
                     /locus_tag="AC16FU_05105"
     CDS             complement(804..1319)
                     /locus_tag="AC16FU_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993785.1"
                     /translation="MVIATACRNPFAPPEAGPARSGPLARQTDPDSTLANFRYAYERR
                     DLDVYENCLASNFVFLYIDQDLTGQIEQVAVPRDGASGDLARTERLFRVFDAVSLDTW
                     NVIAEPDDSTATGVVKRRRVNFHLSVQDLTGDFNFDEYQASGFARFTFEQSKTDNLWR
                     IVTWEDQSFTQ"
     gene            complement(1352..1918)
                     /locus_tag="AC16FU_05110"
     CDS             complement(1352..1918)
                     /locus_tag="AC16FU_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993786.1"
                     /translation="MSLALLVGACKNPFSTREAEKPLGQTGTWSFPASPDLVLRNLLF
                     SFNERVVANYTTCFSSDFIYAVDFFISGVPDTSRSWGNAAELELASKLFNFASAHVDS
                     LNYVLSLAPDPSRTDIRQDTLAIFFRTYQILQLRLKPQPPETTVYAGVATFHLRQDSF
                     NFWALDRWDDQHQSSTIPSWGDLKASFR"
     gene            complement(1971..3818)
                     /locus_tag="AC16FU_05115"
     CDS             complement(1971..3818)
                     /locus_tag="AC16FU_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993787.1"
                     /translation="MAQLRRLFSILALFWLGGGAFTRASQVQFNFTQGNRTYSLTDSL
                     LLERNFSDHWKLSLTNQSNLLLVKKAFLGGTRDRLQKYATSQIALGYQLSGTAEVGVA
                     LGREITSTDPLGYALSRFSGMLKWTPRNEFSLTTLLGLADQSKDSDLGKKGNSGPHWR
                     SQVRWQKVLTKVGSVHLGASQNFLELKRLPARESNLSAALERKGGLADSLTVELHQRW
                     AKKTYYFDERALSATARQNVNSSFIDGKLSRRWPGDWLAAFEYQYNRQENNYRPESVS
                     DVLRDTLALDLQRRKNILWNQEVRATVGKQLKNVAGVTLGWHWEKGAEDYGVNFKDQR
                     RETGELSGTVGLRPSSSDTVAVFGSIGVTSYFVPPISGFLSDRDLLNGTVSLSAGHRF
                     SPYFDLKLTGSRRLLKQIYLSGRYSANNRTTDNYLLAPVLSYRFPGGFALRQGFNLGA
                     NYVTFDFLKTSPNADHTILRQASSASEMTLPLTARLAWILGYTYRYEDFGGLLWRDQW
                     VEQTTWDRRTNIVDARFTYQWAKSLGVSGGFIWEQKFTYDHVARLSTNTIERIRSLSF
                     IRKTVTFGLTYALDGANFLRADYQRRAQSGSGFSVGDTDWATVSYGRTF"
     gene            complement(3820..4689)
                     /gene="rsmA"
                     /locus_tag="AC16FU_05120"
     CDS             complement(3820..4689)
                     /gene="rsmA"
                     /locus_tag="AC16FU_05120"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR2993788.1"
                     /translation="MPKLKRTAERFSASTLVASELTKPHLVPRKSLGQNFLVDRRVQE
                     KIAAALSLKKSDVVVEIGAGRGELTRFLAEQAGAVYAVEIDRRLLPALQSLTKERATV
                     EIIPGDILTVNPREITGDRAVKVAGNIPYHLTSPIIDWLIAQKRRIELAVLLLQREVA
                     RRITGKPETKDWGPLAIAVTCHFHAKKLFDVKPGSFYPPPRVMSSLVEFFTRTEPKVP
                     ARIEEKLLEIVRRAFAHRRKTAINSLILSGGYSAEELVPAFREVNLSPKARGEEITFD
                     QFVRLTEALQKLR"
     gene            complement(4631..5407)
                     /locus_tag="AC16FU_05125"
     CDS             complement(4631..5407)
                     /locus_tag="AC16FU_05125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR2993789.1"
                     /translation="MIDTHAHLDFPEFGEDLEAVLARASASGVKNIVTIGIDGESNRK
                     AVALAGQFPQVYAAVGCHPHEADHWSEEFLNDLEKLARHPKVVAIGETGLDFYRNRAT
                     PENQMRAFDAQIDLAIRLKKPLVIHIRNAFERAFTHLVERAEGKTTVVLHCFQEGEEG
                     ARRAVNAGFFLSFNGTLTYKSSALPEVAKALPLESILLETDCPFLPPVPYRGKRNEPA
                     YLSLVLEKLKTVRPARTRAEIDAQIEKNSRAVFGFDSSGL"
     gene            complement(5471..>6646)
                     /gene="metG"
                     /locus_tag="AC16FU_05130"
     CDS             complement(5471..>6646)
                     /gene="metG"
                     /locus_tag="AC16FU_05130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR2993790.1"
                     /translation="WWNQAKVVHLMAKEILKFHAIYWPALLLAAGIKPPEFLFIHGFF
                     SVNGQKMSKSLGNVIDPHHLVQRYGPDGTRFLLLTQFPFGVDGDIQAERFTEKYNADL
                     ANDFGNLASRVFKLVADHFNGEVPQPGKPTPEDKDFHKIALNLAEKAATAYASFAGEN
                     GRYVYNDALAYTSGLVKEANRYMEAQAPWALAKGGKREQLATVLYYALESLRLAALLY
                     EPALPNKCAELKRALGESQPTGAPFAKGPQWGLLKPGAKISLTGSLFPRLEQEKVKMQ
                     TMAGDDQKNQVTIDEFGKLDLRVAQVTGAQKVAGADKLLQLEVEVAGEKRQVIAGVAQ
                     HYTPEVLLGKKIVVVWNLKPAKIRGLESQGMLLAAQADGQLVLVTVDGEIKTGAKIS"
BASE COUNT         1381 a         1809 c         1984 g         1472 t
ORIGIN      
        1 tttaacattt tgagtcaaat tgaaaaatgc tgttaaattt agtgtatctt actgactatc
       61 aagcagttaa tttggacatc tctccctaat aatctgtatc atttctttaa cggcttggga
      121 gaaccccacc aagaccgccc gcgcgacgat ggcgtggccg atggcgtact cggtgatttt
      181 ctcaatctcc aaaagcggca cgatgttccg gtagttcagc ccgtggccgg cgtggacttc
      241 gatgcccaat ttgtcggcca tatcggtcat ctggtctatt tttttcagtt cttcatcgat
      301 ttctttgtcg gttttgctct cggcgtagcg ggcggtggaa agttcgataa tctccgcgcg
      361 cagtttggcc gcggccttga tctgctcgac ttccggatcg atgaacaaac tcacccgaat
      421 gccggttggt ttcatttgtt tcaaaactgc gccaatccgc tcggaaaaac cggtcacgtc
      481 cagcccgccc tcggtggtca actcctcgcg tttttccggg acgagcgtca ccagatgcgg
      541 cttcacttcc aaagcccgct ccaccatttg cgggatggcg gccatttcta agttcagttc
      601 ggtcttgacc acttccttca agacgtagag gtcgcggtcg cggatgtgcc gccgatcttc
      661 gcgcagatgc acggtgatgc cgtcggcgcc ggccaattcg gccagcatcg cggcaaagac
      721 cggatcgggc tctttgattt tgcgcgcttc gcgcagggtg gcgacgtggt caacatttac
      781 ggagagtttg ggcatagcgg tttttattgg gtaaacgact gatcttccca ggtgacgatg
      841 cgccagaggt tgtcagtttt cgactgctcg aaggtgaagc gggcaaagcc ggaggcctgg
      901 tattcgtcga agttgaagtc gccggtcaaa tcctgcaccg aaagatggaa gttcacccgc
      961 cgccgtttga ccacaccggt cgccgtcgaa tcgtccggct cggcaataac gttccaggtg
     1021 tccagactga cggcatcgaa gacccggaag agccgctcgg ttcgggccag gtcgccggaa
     1081 gcgccgtcgc gcggcaccgc cacctgttcg atctggccgg tgaggtcctg gtcaatatag
     1141 aggaagacga aattggaggc aagacagttc tcgtagacgt cgaggtcgcg gcgctcgtag
     1201 gcgtagcgga agttcgccaa agtcgagtcg ggatcggtct gccgggccaa aggtccggaa
     1261 cgggccgggc cggcctccgg cggcgcgaag ggattccggc aggccgttgc aataaccaat
     1321 agaagaaaaa ttaccgcaag gagccgccgc ctcaccggaa actcgctttc aggtcgcccc
     1381 aacttgggat agtgctcgat tgatgctggt cgtcccagcg gtcgagggcc cagaagttaa
     1441 aactatcctg ccgcagatgg aaggtcgcca cgcctgcgta caccgtcgtt tcgggcggtt
     1501 ggggtttgag gcggagctgc aaaatctgat aggtgcggaa aaaaatggcg agcgtgtcct
     1561 gccggatgtc ggtgcgagac ggatcgggcg ccagtgaaag aacgtaattg agcgaatcga
     1621 catgggcgct ggcgaaattg aagagtttgg aggcgagttc gagttcggca gcgttccccc
     1681 aggaacggga agtgtccgga acgccggaaa taaagaaatc gacggcgtag atgaaatcgc
     1741 tggaaaagca ggtggtgtaa ttggccacta cgcgttcgtt gaaggaaaag aggagattcc
     1801 gcagcaccag gtcgggcgag gcggggaaag accaggtacc ggtctgtcct aagggttttt
     1861 cggcttcgcg ggtagagaaa ggattcttac aggcgccgac cagaagggca agcgacacaa
     1921 tcacggctgt cgcagaagtt ggacgtttga gaaagttaga gccaggcata tcagaatgtt
     1981 ctcccataag aaacggtggc ccagtcggtg tcgcccaccg agaacccgga accggactgc
     2041 gcccggcgct ggtaatcggc gcgcagaaag ttggcgccat ccagtgcata ggtcaagccg
     2101 aaagtgacgg ttttccgaat gaaactgagc gagcgaatgc gctcaatggt attcgtgctc
     2161 aatctggcga cgtgatcgta ggtgaacttc tgctcccaga tgaagccgcc ggaaacgccc
     2221 aacgatttcg cccactggta ggtgaaccgg gcgtccacga tgttcgtccg ccggtcccag
     2281 gtggtctgct cgacccactg gtcccgccag agcaagccgc cgaaatcttc gtagcggtag
     2341 gtgtagccca gaatccaggc aagtcgtgcg gtcaaaggca gggtcatttc gctggccgaa
     2401 ctggcctgac gcaaaatcgt gtggtcggca ttgggcgagg ttttcagaaa atcgaaggtg
     2461 acgtagttag ctcccaagtt gaacccctgg cgcagggcaa agccgcctgg gaagcggtac
     2521 gagagcacgg gagccaggag gtagttgtcg gtagtgcggt tgttggcgga gtagcggccc
     2581 gacagataaa tctgtttgag caagcggcgg gagccggtca gtttcaggtc aaaataggga
     2641 ctgaagcggt gcccggcgga aagcgaaacg gtgccgttca aaagatcgcg gtcagaaaga
     2701 aagcccgaga tcggcggcac gaagtaggag gtgacgccga tggaaccgaa gaccgcgacg
     2761 gtgtccgaac ttgatgggcg cagaccgaca gtaccagaga gttcgccggt ttcgcgcctt
     2821 tggtctttga aattcacgcc gtaatcttcc gcgccttttt cccagtgcca gcccagggtg
     2881 actccggcaa cgtttttcaa ttgtttccca acggttgcgc gcacctcttg attccagagg
     2941 atgttcttcc gccgttggag atccagtgcc agcgtgtcgc gcagaacgtc ggaaacggac
     3001 tcgggccggt agttattttc ctgacgattg tactggtact caaacgcggc caaccagtcg
     3061 cccggccagc ggcgggagag ttttccatca atgaaactgg aattaacgtt ttgcctggcg
     3121 gtggcggaaa gcgcgcgttc gtcgaagtag taggtcttct tcgcccagcg ctggtggagt
     3181 tccacggtta aggagtcagc caaaccacct ttgcgctcca gcgcggcgga gaggttgctc
     3241 tcgcgagccg gcaggcgttt taattccaga aaattctggc tggcaccgag gtgcaccgaa
     3301 ccgactttcg tcagcacctt ttgccaccgc acctggctcc gccagtgcgg ccccgagttt
     3361 ccttttttgc cgaggtcgga atccttgctc tgatcggcga ggccgagaag ggtcgtcaga
     3421 gaaaattcgt tccgcggcgt ccacttcagc ataccggaaa agcgggaaag agcgtacccg
     3481 agcggatcgg tggaagtgat ttcgcgcccc agcgccacgc ccacttccgc cgtgccggag
     3541 agttgatatc ccagggcgat ttggctggtg gcgtattttt ggaggcgatc ccgggtgccg
     3601 ccgagaaagg ccttcttgac caggagcagg ttcgactggt tggtgaggga aagtttccag
     3661 tggtcggaga aattccgttc cagcagaagt gaatcggtta agctgtaggt gcggttgccc
     3721 tgggtgaagt tgaactgcac ctgggaggca cgggtgaaag caccgccgcc gagccagaaa
     3781 agggccaaaa tcgagaaaag tctgcggagc tgcgccattt tatctcaatt tttggagtgc
     3841 ttcagtcaga cgcacgaact gatcgaacgt tatttcttcg ccgcgcgctt tcggggagag
     3901 attcacctcc cggaacgccg ggaccaattc ttcggctgag tagcctccgc tcaagatcaa
     3961 agaattaatg gcggtcttgc gccggtgggc aaaggcccgg cggacgatct ccaaaagctt
     4021 ttcttcgatc ctggcgggca cctttggctc ggttcgggta aaaaactcga ctaaactgga
     4081 catcactctt ggcggcggat agaaactgcc cggtttgacg tcgaaaagtt ttttggcatg
     4141 gaagtgacag gttacggcaa tggccaaagg tccccaatct ttagtttccg gtttgccggt
     4201 gatgcggcga gccacttccc tttgcaacaa caaaaccgcc agttcaatcc gccgtttttg
     4261 tgcgatgagc caatcgataa tcggactggt gagatggtag gggatgtttc cggctacctt
     4321 caccgcccgg tcgcctgtga tttctctggg gttcactgtc aaaatgtcac ctggaataat
     4381 ctctacagtt gcccgttcct ttgtcagcga ttgcaatgcg ggaagcagac ggcggtcaat
     4441 ttctaccgcg tacaccgctc cggcttgctc cgccagaaaa cgggtcaact caccccggcc
     4501 cgcgccgatt tccaccacca cgtcggactt tttcagcgaa agggcggcgg ctattttttc
     4561 ctgaacgcgc cggtcaacga gaaagttctg ccccagactt ttcctgggga cgagatgcgg
     4621 tttggtcagt tcagaggcca ctagagtcga agccgaaaac cgctcggctg ttcttttcaa
     4681 tttgggcatc gatttctgcg cgggtgcgcg cgggccgaac tgtttttagt ttttctaaca
     4741 ccaaagacag gtaagccggt tcattgcgtt ttccccggta gggaacaggt ggcagaaaag
     4801 gacaatcggt ttccaagagg atagattcca gcggcagcgc tttggccact tcgggcagcg
     4861 cactgctctt gtaggtgagc gtgccgttga aggagagaaa aaatccggca ttcacggcgc
     4921 ggcgggcacc ttcttctccc tcttgaaaac agtgcagcac caccgtcgtt ttcccctcag
     4981 cacgttccac caggtgcgta aaggcccgtt cgaatgcatt ccggatgtga atgaccagag
     5041 gttttttcag ccggatggcc aggtcgatct gcgcgtcgaa cgcccgcatt tgattttccg
     5101 gcgtggcgcg attgcggtag aaatcgagtc ccgtctcacc gatggcaacc actttcgggt
     5161 gccgggcaag tttttccaaa tcgttcaaga attcttcaga ccagtggtcg gcttcgtggg
     5221 ggtggcagcc gacagcagcg tagacttgtg gaaactgtcc ggccagtgct actgctttgc
     5281 ggttcgattc gccatcaatg ccgatggtga caatgttctt cacaccgctg gcggaggcgc
     5341 gcgcaagcac cgcttccaaa tcttcgccaa attcggggaa atcgagatgg gcgtgagtgt
     5401 cgatcatgcg aaaaccaaaa acgtcccgcc tttggcggga cgtccgtacc gagatgctgt
     5461 gcgggggagg tcagctaatc ttcgcaccgg ttttgatttc tccatccacc gtcaccagca
     5521 ccagttggcc atcggcctgg gcggccaaaa gcattccctg cgactccagg ccccggattt
     5581 tggccggttt caagttccag acgaccacaa tttttttacc caggagcact tccggtgtat
     5641 agtgttgcgc cacgccggct atgacctggc gtttttcacc ggctacttcc acctcaagtt
     5701 ggagcagttt atcggcgccg gctacctttt gggcgccggt cacctgcgcc acccgcagat
     5761 cgagtttgcc gaattcgtca atggtcacct gattcttctg gtcgtcgccg gccatggttt
     5821 gcatctttac tttctcctgt tccagccgcg ggaaaagcga accggttagg ctgattttcg
     5881 ctcccggttt gaggagcccc cattgcggcc ctttggcgaa gggagcgccg gtcggctggg
     5941 attcacccag cgcccttttc aactccgcgc acttgttggg gagcgccggt tcgtagagga
     6001 gggccgccag gcgcagactc tccagcgcgt agtaaagcac cgtggcgagt tgctcccgct
     6061 tacccccttt cgccagtgcc cagggggcct gtgcctccat gtagcggttg gcttctttga
     6121 ccaatccgct cgtgtaggcc agggcatcgt tgtaaacgta gcgtccattt tcgccggcaa
     6181 aactcgcata cgcagtcgcc gctttctcgg ccaagttcag agcgattttg tgaaagtctt
     6241 tatcttcagg cgtcggcttc cccggctggg gaacttctcc attgaagtgg tcggccacta
     6301 acttgaaaac ccggctggcc aagttgccga agtcgttggc taagtcggcg ttgtactttt
     6361 cggtgaagcg ttcggcctga atatcgccgt ccacgccgaa ggggaactgc gtcaaaagca
     6421 aaaagcgggt cccgtccggg ccgtagcgct gcacgagatg atggggatcg atgacgttcc
     6481 ccagactctt gctcatcttc tgcccgttga ccgagaaaaa gccgtggatg aacaagaatt
     6541 ccggcggttt gatcccggcc gccaaaagaa gcgcgggcca gtagatggca tggaacttca
     6601 aaatctcctt ggccatcaga tgaaccactt tcgcctggtt ccacca
//
LOCUS       DBNGZA010000243         2223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_788162, whole genome shotgun sequence.
ACCESSION   DBNGZA010000243 DBNGZA010000000
VERSION     DBNGZA010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_788162"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="AC16FU_05135"
     CDS             complement(<1..1221)
                     /locus_tag="AC16FU_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2993791.1"
                     /translation="MAILLFAWGGILASPVAYSQGFVLKYRIDGVADNFNLGYSVAGA
                     GDVNGDGYSDFIIGAPGRGVSSGLALVYSGRTGQLLHVLYGSDGERFGFSVASTGYVN
                     QDRTTDFIIGAPYANPPGLLHAGSVYLYSGATGELIRRINGTAAGDHVGWSVGGFGDV
                     NEDGRADFLVGAPGFFGEIPGFGSAIVYSGKIGTVLYQQRGVSGENLGYSVAEVGDLN
                     GDGRADFIVGAPLANPPGYPATGGSAFVYSGATGNLLFQIDAATDDDQLGFSVAGAGD
                     VDGDGRADFIIGAPAHDPGEITNAGSAFVYSGATGTLLYQKDGSAAGDNLGYAVAIAG
                     DVNGDGRADFLVGSPFAAPSGLAYAGSAFLYSGRDGSFLFQIDGTAAGDQLGQSVAGA
                     GDLDGDGRAEFIVGA"
     gene            1887..>2223
                     /locus_tag="AC16FU_05140"
     CDS             1887..>2223
                     /locus_tag="AC16FU_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993792.1"
                     /translation="MTNTSNLLGQIQAELAEQSEQVTSDLVALINQRIRPPVPVTIEN
                     VHIRTMFVISDQVNSYGGRLPLEEHPNVARLLVDAPVLIGHAKDRLPVGRNFKAELVD
                     FWAAWCGPCK"
BASE COUNT          527 a          707 c          540 g          449 t
ORIGIN      
        1 cgcgccgacg atgaattcgg cccgcccgtc tccatccaga tcccccgccc cggccaccga
       61 ctggcctaat tgatcccccg ctgcggtgcc gtctatctga aagaggaacg agccgtcccg
      121 gccggaatag agaaaggccg agccagcgta tgcgagcccg ctcggagcgg caaacggcga
      181 ccccaccaga aaatcggctc tcccatcacc attcacatct ccggcgatgg ccactgcata
      241 gcccaaatta tcacctgctg cacttccgtc tttctgatac aaaagcgttc cagtggctcc
      301 ggaatagaca aaggccgaac cggcgttcgt aatttcacct gggtcatgag cgggagcgcc
      361 aatgataaaa tcagctcttc catcgccgtc cacatccccg gctccggcta ctgaaaaacc
      421 taactggtcg tcgtcggtag cagcatctat ctggaacaac aaattcccgg tagctcccga
      481 ataaacaaat gcggaacctc ctgtggcggg gtatcccggc ggattagcca aaggtgcacc
      541 aacaatgaag tcggctcttc catcgccatt cagatctcct acctctgcca ccgaataccc
      601 cagattctcc ccgctgacgc ctctttgttg atacaaaacg gtaccaatct taccggaata
      661 aacgatggca gaaccaaatc ccgggatttc cccgaagaaa cctggagcac ccacgagaaa
      721 atcggctctc ccatcctcat tcacgtctcc gaatcccccc accgaccatc ccacatgatc
      781 gcccgcggcc gtgccgttta ttcgacgaat aagttcgccg gtggctcccg aatagagata
      841 gaccgaaccg gcgtgtagta gtccgggcgg attggcgtaa ggtgcgccga tgataaaatc
      901 ggttgttctg tcttggttca cataacccgt actcgccacc gaaaaaccga accgctctcc
      961 atcgctcccg tagagcacat gtagcaattg tcccgttctg ccggaataga ctaaggccag
     1021 gccggatgat acgccccgcc ccggcgcacc aatgatgaaa tcgctatagc catcaccatt
     1081 tacgtcgccg gctccggcaa ccgaataccc cagattgaaa ttatccgcca ccccatcaat
     1141 gcggtacttc agcacaaagc cctggctgta cgcaacggga gaagccagga ttccgcccca
     1201 ggcgaaaagc aaaatcgcca taagaactaa tcgtgtcgga aatgggatta ctttgcgttg
     1261 cctttgactg cccacaagaa aaccgcttgc catacgcata acggctcctt ttccagcagt
     1321 ggcgagatta tcgtccgcgc cggtggctta aactccaccg cctcccctcg tggatcaagt
     1381 tacctctttt ggcagccggt tgtcaagaag ttttttccac cgccctgttg actataaatc
     1441 gagccccact gactcatcac ccccgagccc ctaatcactt gtcacgactc actgtttttt
     1501 tcggggtcat cccggggggt aaaacgaact tccccttttt tctttcccat aggcggcgct
     1561 atcagcccgc cctacctgtt ttaagatgtt ttcgtgccca ccgcctccaa tgtcacccat
     1621 cccatctcat tgcgctgcac cttaatccgc ttaatccgct tcaaaatccg ttgtcagaat
     1681 ttggggtcgc cacgggggta aaacggacaa agtgcgaaat agtagaggcc ctggttgtag
     1741 caaccgggtg ccaaaaatgt agaggcggat ccgcctcagg cggatgttcg ccaaaaccaa
     1801 accgtagtgc gggatggtct tcgtatcccg cacggaaaag aaaacgtagg ggcgaacccc
     1861 cgtgttcgcc cccaacaaac ggaacgatga caaacacatc aaacctcctc ggccaaatac
     1921 aggcagaatt agcggaacaa agtgaacagg taacgagcga cttggtcgcc ctcatcaatc
     1981 agcgcatccg gccgcccgtg ccggtgacga tcgaaaacgt ccacatccgc acgatgttcg
     2041 tgatatccga tcaggtcaac tcctacggcg gacggctgcc cttggaggaa catccgaacg
     2101 tggcgcggct tttggtcgat gcgccggtgc tcatcggcca cgccaaggac cggttgccgg
     2161 tggggcggaa cttcaaggcc gagttggtgg atttctgggc agcgtggtgt ggcccctgca
     2221 agg
//
LOCUS       DBNGZA010000244         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1104891, whole genome shotgun sequence.
ACCESSION   DBNGZA010000244 DBNGZA010000000
VERSION     DBNGZA010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1104891"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="AC16FU_05145"
     CDS             <1..1517
                     /locus_tag="AC16FU_05145"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR2993793.1"
                     /translation="FVFTYSFWIQATRAEVYSLNILFFSILFFLAVRLALMPPAGAPP
                     STPTLALFGYLTALGLGNHHAILISTLPAFGFLFFRLYGRALLAPRRLFLWAALAAVG
                     ISIYLYLPIRSAQEPLLHWGSQSSAGSTVAGVIAADSFSEFSWRTLTSLPQKFGQIGV
                     LFWDQFTPLLLVVVLGGWVALWGKSRTWWFFSSLLLLGNALMAAVLSDEIILDNPDLH
                     GYLQPSILTLGLWFGVGIFAALALVDRTAARWHARPDFRWLLSAVFSGFFFLIGFLPW
                     WSNRSYADLSANRLAERVGLSVLEPLPEHAVAFIDNPNLDFILRGLQIAENKRPDVIV
                     VDRALLGGSWYVRQTRRNFPFLFAGVPEHLAGESLFVILRDGCLEQGIPAFMEFTEHD
                     ISFVHHLMPSGYLYQIRRDSVGEIPKEVMLYQKEWARKQFINDDDVSRLHDVDGIRML
                     VLTWFRTGLFFEAKGLRPQAQAFYRKTRRAARPEKALPDHPQNPADNADKIPKP"
     gene            1644..2060
                     /locus_tag="AC16FU_05150"
     CDS             1644..2060
                     /locus_tag="AC16FU_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2993794.1"
                     /translation="MSKGFTLIELLIVVIIIGVLAAAAIPSFLRASTKSKQAEAKQFL
                     KEIYTMELAYRHSQDTYIAYGGVRGGGSLPEIGVEIPTSAKYIYSVTAGPTGIATSFI
                     ATATVPAPGLDDDPAPDIWQIDDQGVLQPVSNDAEQ"
     gene            2341..>2671
                     /locus_tag="AC16FU_05155"
     CDS             2341..>2671
                     /locus_tag="AC16FU_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR2993795.1"
                     /translation="MEKGFTLIELMIVVVIIGILAALAIPRFMRAATKTKQSEAKQIL
                     KQIYVQEHAYRQEKDFYIAYPPTGAGGTMPEIIIEIMPSARYTYSVAAGGSGIVNSFV
                     ATATANLD"
BASE COUNT          502 a          787 c          774 g          608 t
ORIGIN      
        1 ccttcgtgtt tacctacagc ttctggatcc aggccacgcg ggccgaagtt tattcactca
       61 atatcctttt cttctcgatc cttttctttc tggcggtccg cctcgctctg atgcctccgg
      121 cgggagcgcc gccgtccact ccgacgctcg cccttttcgg gtacctgacc gcactgggac
      181 tggggaatca tcacgcgatc ttgatctcca ccctcccggc gttcggattt ctttttttcc
      241 gtctctatgg ccgcgccctt ctggcgccgc ggcgtctttt cctctgggcg gctttggcgg
      301 ccgtggggat ttcgatttac ctctacctgc ctatccggag tgcccaggag ccgctgttgc
      361 actggggttc tcaatcctcg gccgggagta ccgtggccgg agtgattgcg gcggattcat
      421 tcagcgagtt ctcctggcgc acgctgacca gtctgccgca gaaattcggc cagatcggcg
      481 tgcttttctg ggaccaattc acgcccttac tattggtggt cgttttgggg ggctgggtgg
      541 cgctctgggg aaagagccgc acctggtggt tcttttcgag tttgctcctt ctgggcaacg
      601 cgcttatggc ggcggttctc tccgatgaaa ttattctgga taatcccgac ctgcacggct
      661 acctccagcc gtcgatcttg accttggggt tgtggtttgg cgtcggcatt tttgccgcgc
      721 tcgcactggt agaccgcacg gcggcacgct ggcacgcccg gcccgatttc cgctggcttt
      781 tgtccgccgt cttcagcggc tttttctttt tgatcgggtt tctgccgtgg tggtcaaacc
      841 ggagttacgc cgatctgtcg gccaaccgcc tggccgaacg ggtgggtctt tcggttttgg
      901 agccgctgcc ggaacacgca gtcgctttca tcgacaatcc aaatctcgac ttcatcctgc
      961 gcggcctcca gatcgccgaa aacaaacggc cagacgtcat tgtagttgac cgcgcccttt
     1021 tgggcggcag ctggtacgtc cgtcagaccc gccgcaattt tcctttcctc ttcgctggtg
     1081 tgccggagca cttggcggga gaaagtctgt tcgtcatcct gcgcgacggt tgcctggagc
     1141 agggtattcc cgcgttcatg gaattcaccg aacacgacat ctccttcgtg caccacttga
     1201 tgccctccgg ctacctgtac caaatccggc gcgactcggt cggtgaaatt cccaaagagg
     1261 tgatgctgta ccagaaggag tgggccagga aacagttcat taacgacgac gacgtttccc
     1321 ggctgcatga cgtggacggc atccggatgt tagtgttgac gtggttccgg acgggtcttt
     1381 tcttcgaagc caaaggcctc cggccgcagg cgcaggcctt ctaccgtaaa acccggcggg
     1441 cggcccgtcc ggaaaaggcg ctccccgacc atccccaaaa tccggcggac aatgccgata
     1501 aaataccaaa gccgtagcgg cacaacggaa actcctatgg agaaaatagc gctggcgtta
     1561 gccatggcgt ttgccgatcc ggctgggccg gagcagttgt gggacctcct gagacgaggc
     1621 gggcgatgac cggtggccga cgtgtgtcaa aaggctttac gctcattgag cttttgattg
     1681 tcgtgattat catcggcgtt ttggccgccg cggcgatccc gagcttcctg cgggcttcga
     1741 ccaaaagcaa gcaggcggaa gcgaaacagt tcttaaagga gatttacacc atggagctgg
     1801 cctaccggca cagccaggac acctacattg cctacggggg tgtgcgagga ggcggctctc
     1861 tgccggagat cggcgtggag attcctacct cggcgaagta catctattcg gttactgccg
     1921 gtcctacggg aattgccact tcctttatcg ccaccgctac cgtccccgct ccgggtttgg
     1981 atgacgaccc ggctccggac atctggcaaa tcgacgacca gggggtcttg caaccggtca
     2041 gcaacgacgc cgagcaataa gccgtcaccg cctttcgttc agaagcgttg ctccccgccg
     2101 ttttctctga cccgtcacgc aactgtggca aattgcaaag gcagtagcag gaaaagtcac
     2161 cagcagtgtg ggggaaaagc cagaccaacc tccggtaagc agaagtacaa ccgacggtta
     2221 tccaaaaacg gcgggcacgg aacggcggtt gctctttcgg gaagtgacaa ggtgtaccct
     2281 tgggtagact cagggcacac ataagcaaag tcgaggtgtg ctatgaccag aaggcgacga
     2341 gtggaaaaag gttttaccct gatcgagttg atgatcgtgg tagtcatcat cgggattttg
     2401 gcggcgttag ccatcccgcg cttcatgcgg gctgcgacga aaaccaagca gtccgaagcg
     2461 aaacagattt tgaaacaaat ctacgttcag gagcacgcct accggcagga aaaggatttt
     2521 tatattgcct atccccccac gggtgccggc gggaccatgc cggaaattat catagagatc
     2581 atgccgtcgg cccgctacac ctactcggtg gccgccggcg gctcggggat cgtcaattcc
     2641 ttcgtggcga ccgcaaccgc gaacttagat g
//
LOCUS       DBNGZA010000245         4346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_470448, whole genome shotgun sequence.
ACCESSION   DBNGZA010000245 DBNGZA010000000
VERSION     DBNGZA010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_470448"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16FU_05160"
     CDS             <1..348
                     /locus_tag="AC16FU_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR2993796.1"
                     /translation="IILLSDLFDDPDRVLSGLKHFRHRKHEVILFHILDPLERTFAFP
                     GEAVFKDMETGEEITTLPWQIKRDYQARLKTLTDRYLYECRKSQIDYQQLDTATPFDK
                     ALLAYLIKRGRLY"
     gene            387..743
                     /locus_tag="AC16FU_05165"
     CDS             387..743
                     /locus_tag="AC16FU_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2993797.1"
                     /translation="MQKIESFQQLNVWQASHRLALEIYLLTKQFPREEKFGLVSQMRR
                     AAVSVPANIAEGFKKRGTKDKINFYNISQGSLEELRYYLILSRDLGYLKDHAATEQKT
                     EIISRMLTALIRSLSE"
     gene            complement(791..1255)
                     /locus_tag="AC16FU_05170"
     CDS             complement(791..1255)
                     /locus_tag="AC16FU_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR2993798.1"
                     /translation="MRLLNLILAWLHLISAFTLVGGSIFVGFVLAPAAKAQSGNWIVT
                     LAATEKRYRKLARRMVEVVILTGVANLLLHRLAFHMPWTGHYLGTIALKLALVLTMVF
                     LYARRVKILTALADKGTLGEVENNELKRADQLIGWQMVLGAVVILLGLSLRG"
     gene            complement(1313..2203)
                     /locus_tag="AC16FU_05175"
     CDS             complement(1313..2203)
                     /locus_tag="AC16FU_05175"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR2993799.1"
                     /translation="MKTAKETAIESEPGVLIQVEEQPVPATETSVGIKHAIAVTSGKG
                     GVGTSAVAVNLAVALAQQGLEVGVVDFDHDGPSVAKMLGIEPQKFAAGDNGVLPVTGP
                     LGIKVLTFDLCQSAAAPQNSATSENGVVSNGTPAPNLAVALANTDWGALDYLFIDVPS
                     ETDDLLQVARTIPALDGILVVTIPAEISHLIVKKSIALTRGVTAAPVIGLVENMQGYF
                     CTHCGQTGELFPQGNGRVTARELGIPYLGGIPFDPRLADAGDRGAPYLVTYRDTATGQ
                     AILKIAKSVTQFFADKNHKS"
     gene            complement(2244..3392)
                     /locus_tag="AC16FU_05180"
     CDS             complement(2244..3392)
                     /locus_tag="AC16FU_05180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR2993800.1"
                     /translation="MLTKAEPVLKTEKEKRLAAHEIEHRTGHPYHMYDSILLQPTMVR
                     EALAANEKNVGKVAGALAKKKRLYLVGIGTSSHAAIAGEYFFRVLCGGKLDAQFIQAF
                     EFVHYPPRLDRETAVLIVSHRGWKTFSEQATKMAREQGALSISVTGRNPQREVILGEH
                     VLFTSEQEKSHAHTMSSTAALAVLLQLAQATGKRVGAKQQETFELSNLPDLLEASLAL
                     EPQTRNLAGRLCDCERFFFVAFGPSVATALEAALKMQETTFTASAGYEVEQFLHGPLA
                     SVSPESAVFFIAPPGLGQKRCWQALNAVKAVGAKTVLLTQKNEISLAPAADETILLPE
                     MNELVTPIVYVVPVQLYSYFTAIKLGHNADVNHRDDLRYQKASEAFAL"
     gene            complement(3500..>4346)
                     /locus_tag="AC16FU_05185"
     CDS             complement(3500..>4346)
                     /locus_tag="AC16FU_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2993801.1"
                     /translation="LQVEYAINEGGRIRTDDSGQVRYVCIQNTEKVPYNLTLKVKGTP
                     GHASVPLPDNPIFALSQALAKIVNYKAPLKLIPTTEKFFAGLAKKEKYPASYYLENAN
                     HPLVGQLCREALAKMHPVFHSMLCNSISPTVFQAGIRANVIPSEAQVNLNCRLLPGEK
                     ITHFVEEVKRVISDPRIEITYEVGKQPDSPESPVDSPVYRALETNAKKLWPEAEVVPL
                     LSTGATDSAELRAIGVKSYGILPFPLSENDEARMHGHDERIPLDGYLEGIKYLFNTTV
                     ELAAE"
BASE COUNT          940 a         1229 c         1202 g          975 t
ORIGIN      
        1 attattctcc tttccgacct ctttgacgat cccgaccggg tgctctcggg actcaagcac
       61 ttccgccacc gcaagcacga ggtgattctc ttccatatcc tcgacccgct ggaacgcacc
      121 ttcgccttcc ccggagaggc ggtctttaag gatatggaaa cgggtgaaga gattacgact
      181 ctcccctggc agatcaaaag agactatcaa gcccgcttga aaacgttgac cgaccgctac
      241 ctctacgagt gccgcaaaag ccagatcgac taccagcagc tggacacggc cacgccgttc
      301 gataaagcgt tattagcgta tttgattaaa cgcggtcggc tgtattaagc ccgaaacacg
      361 gcacacggta tacagcacac cgggaaatgc aaaagataga gagttttcaa cagctcaatg
      421 tctggcaggc aagccaccgg ttagcgctgg agatctacct gctgacaaaa caatttcctc
      481 gtgaagaaaa attcggtcta gtgtcgcaga tgcgccgggc ggccgtctca gtgcctgcaa
      541 atattgcgga aggttttaag aaacgaggga caaaagataa aataaatttt tacaacattt
      601 ctcagggctc tttggaagaa cttcggtact atctcattct ttcacgggat ctcggatact
      661 tgaaggacca tgccgccacc gaacaaaaga cggaaataat ttcccgaatg ctcaccgctc
      721 tcatccgttc tttatctgag tagttccccg tgtgctgtgt actgcatact gtattctaaa
      781 gcgaaatacg ctatccccgc aaacttagac caagaagaat caccaccgca cccaagacca
      841 tctgccagcc aataagctgg tcggcgcgtt tcagttcatt gttttcgacc tcacccaatg
      901 tgcctttgtc ggccagtgcg gtgagaattt tcacccgccg ggcatagagg aataccatcg
      961 tgagcaccaa cgccagcttc agggcgatcg tgcctaaata atggccggtc cagggcatgt
     1021 ggaaggccag acgatgcaac aggaggttgg ccacgcccgt gagaatcacg acctccacca
     1081 tccgccgggc cagcttgcgg tagcgttttt cggtggctgc cagagtcaca atccaatttc
     1141 cgctctgagc tttcgccgcc ggcgccagca cgaagccgac aaaaatcgag ccgccgacca
     1201 gagtgaaggc cgagatcagg tgcagccagg ccaggatcaa attgagcaga cgcatagagc
     1261 gaattcgcga ggccgaagtt tcgcctctca aggaaccagt tgaaggaaaa tactaactct
     1321 tgtggttttt gtcagcaaag aactgggtga ccgacttggc gattttgaga attgcctggc
     1381 cggtggccgt gtcgcggtag gtgaccaggt acggcgcgcc gcggtcgccc gcatccgcca
     1441 gccgcgggtc gaacgggatg ccgcccaggt agggaattcc cagttcgcgc gcggtcaccc
     1501 gcccatttcc ctgggggaag agctcgccgg tctgcccgca gtgggtgcag aagtacccct
     1561 gcatgttctc caccaagccg atcaccgggg cagcggtcac tcccctggtg agggcaatcg
     1621 atttcttaac gatcaggtgc gaaatttcgg cgggaatggt caccacgaga atgccgtcga
     1681 gagcgggaat ggtgcgcgcc acctgcagca gatcatcggt ttccgaagga acatcgatga
     1741 acaaatagtc aagcgcgccc cagtcggtat tggccagagc tactgccagg tttggcgcag
     1801 gcgtgccgtt ggagactacg ccattttcgg acgtggcgga attctgcgga gctgctgcag
     1861 attgacacaa atcaaaagtc agtactttga ttcccaaagg accggtgacg ggcagaacgc
     1921 cattgtcacc ggccgcgaac ttctgcggct cgatacccag catcttcgcc actgaaggac
     1981 cgtcgtggtc gaaatcgacc acgcccactt ccaagccctg ctgggccaga gccaccgcca
     2041 gatttacggc aaccgcgctg gtgccgacgc cgcctttgcc gctggtgacc gcgatggcgt
     2101 gcttgatccc aaccgacgtt tcggtcgccg gcaccggttg ctcttccacc tggatcaaca
     2161 cgccgggttc tgattcaatc gcggtttctt tcgcggtttt catttcttcg ttcctgcttt
     2221 cttttttcac cggccggtac gtttcacaaa gcgaacgcct cgctcgcctt ttggtagcgc
     2281 aggtcgtccc gatgattaac atcggcatta tggcccaact taattgctgt aaagtaagaa
     2341 taaagttgca ccggaacgac gtacacaatt ggtgtgacga gttcgttcat ttcggggagt
     2401 aaaatagttt cgtccgctgc aggtgcaagc gaaatttcat ttttctgcgt cagaagaacc
     2461 gtcttcgcac cgaccgcttt cacggcgttc agtgcctgcc agcaccgctt ttgtcccaat
     2521 ccgggcggcg caatgaagaa aactgccgac tcgggactga ccgacgccaa aggcccgtgg
     2581 agaaattgtt cgacttcgta gccggcgctg gcggtgaacg tagtctcctg cattttcaga
     2641 gccgcttcca gcgctgtggc aacgcttggc ccgaaagcta cgaagaaaaa ccgttcgcag
     2701 tcgcagagac gacccgccag attgcgtgtc tgtggttcca gagccagtga tgcttccagt
     2761 aaatccggca gattcgacag ttcaaatgtt tcctgctgct tcgcaccgac ccgtttcccg
     2821 gtcgcctgag ctaattggag taaaactgcc agcgcggcgg tagaactcat cgtgtgagca
     2881 tgtgattttt cttgctcgct ggtgaagagg acgtgctcac ccaaaataac ttcccgttga
     2941 ggatttctgc ccgtcaccga aatcgaaagt gcgccttgct cgcgagccat tttcgtcgcc
     3001 tgctccgaaa aagtcttcca gccccgatgg ctgacgatga ggacggcggt ctcccggtcg
     3061 agacgcggcg ggtaatggac gaactcaaag gcttgaatga actgcgcgtc taattttccc
     3121 ccacagagca cgcggaagaa atactccccc gcaatggcgg cgtgggatga ggtgccgatg
     3181 ccgaccaggt agaggcgttt cttttttgcc agtgctccgg caaccttgcc gacgtttttc
     3241 tcgttggcgg ccagcgcttc ccgcaccatt gtgggttgca acagaattga atcgtacatg
     3301 tggtacgggt ggccggtgcg gtgctcgatt tcgtgcgccg ccagccgttt ttctttttct
     3361 gtcttgagta ccggttcagc cttcgtcagc atctattttc tatttcggac gaaatttcgg
     3421 ataggataca cccgccggtc gtcaaaatga cgagaaaaat ctatcggctc acttccagcg
     3481 gcggctcgtg gagttccact tactccgccg ccagttccac cgtcgtgttg aacaggtatt
     3541 taattccctc aaggtacccg tccagcggaa ttcgctcatc gtggccgtgc atgcgggctt
     3601 cgtcgttttc ggagagcgga aaaggcaata tgccgtagga cttgacgccg atagcgcgca
     3661 gctccgccga atcggtggcg ccggtggaga ggagcggcac cacctccgcc tcgggccaga
     3721 gctttttggc gttggtctcc agcgcccggt agaccgggct gtccacaggc gactccggcg
     3781 aatcgggctg cttgcccacc tcgtaggtga tttcgatccg cgggtcgctg attacccgct
     3841 tcacttcttc tacgaaatga gtaatttttt cgcccggtaa gaggcggcag ttcaagttca
     3901 cctgcgcttc ggagggaatc acgttggccc ggatgccggc ctggaagacg gtcggtgaaa
     3961 tcgaattgca gagcatcgaa tggaaaaccg gatgcatctt tgccagcgcc tcgcggcaga
     4021 gttggccgac cagcgggtgg ttggcgttct ccaagtagta actggccggg tacttctcct
     4081 ttttggccag accggcgaag aatttctccg tcgtggggat cagtttcaaa ggcgctttgt
     4141 aattgacaat cttcgctaac gcctgcgaga gggcaaagat ggggttatcg gggagcggaa
     4201 ctgaggcgtg gccgggggta ccttttacct tgagcgtcaa attgtacggc accttctccg
     4261 tattctggat gcagacgtac cgcacctggc cggaatcgtc ggtgcggatg cggccacctt
     4321 cattaatggc gtactcgact tgcagt
//
LOCUS       DBNGZA010000246         2079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_46912, whole genome shotgun sequence.
ACCESSION   DBNGZA010000246 DBNGZA010000000
VERSION     DBNGZA010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_46912"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="AC16FU_05190"
     CDS             complement(<1..918)
                     /locus_tag="AC16FU_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HIR2993802.1"
                     /translation="MKTFLGIFEDDTYANFFPVTFNRPVWLLRSGIRLLWEKIEAAFP
                     GTTVGFSCRDLLAETAAEQVTGSVNVLPETAERIVLVNGRLAVDRSGADVIAGTQPPA
                     VFLDGETVAAIILDPKTAGAYLSNLRSLWERGAQRQLVDRLPRRPFKGRWTNYLWELV
                     ATNGDEITRDFAALAPTLDFKKMFDRCEVDQQTVIYKVEQVYLGEGAKIDAGAALDAR
                     SGPIFIDREVAIQAQTRVEGPAYIGPRSILVGGRIRSGCSFGPVCRIGGEVEQSIFLG
                     YDNKYHDGFLGHAYVGQWVNLGALTTNSDL"
     gene            complement(1120..1683)
                     /locus_tag="AC16FU_05195"
     CDS             complement(1120..1683)
                     /locus_tag="AC16FU_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2993803.1"
                     /translation="MAAIFFLLVILFATPVVLFIVYYNRLVRLRTGSEAAWSQVDVEL
                     QRRADLVPNLAETVRGYAGHERGVFENVALARSRWQEAKTIPEKAQASEFLAGTLKSL
                     FAVAENYPALLASQNFRELQQQLTEIETRIAAARKAYNEICFQYNTARQTFPSNLVAG
                     MFTFAGLDYFQPEGPAARAVPKVEFGR"
     gene            complement(1684..>2079)
                     /locus_tag="AC16FU_05200"
     CDS             complement(1684..>2079)
                     /locus_tag="AC16FU_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2993804.1"
                     /translation="AKNPKDGTAWLDLAEVYKKTGQPAKAVEAYKQAIAADPQNVEAH
                     YNLGNAYLGKGLYGEAQKEYREVMKLKPVHYKAAFNLAVAVQSQDPESLQQFLPLYEE
                     FVQKFKGNAQARPMVQKAQEIIKQGKGAK"
BASE COUNT          399 a          649 c          576 g          455 t
ORIGIN      
        1 caggtcacta ttcgtggtaa gcgcaccgag atttacccat tggcccacgt aggcgtgacc
       61 taaaaacccg tcgtggtact tgttgtcata ccccaagaaa atcgactgct ccacttcccc
      121 gccgatccgg cagaccgggc cgaaggaaca gccggagcgg atccgaccgc cgaccaaaat
      181 tgacctgggc ccgatgtaag ccggcccttc aaccctggtc tgggcctgga tagcgacctc
      241 ccggtcgatg aaaatcggcc cggagcgtgc gtcgagcgcc gcgccggcat cgattttcgc
      301 tccctcgccg aggtagacct gctcgacctt gtagattacg gtttgctgat ccacttcgca
      361 tcggtcgaac atttttttga aatccaaggt cggcgctaag gccgcaaagt cgcgggtgat
      421 ttcgtctccg ttcgtcgcga ctaattccca gaggtaattg gtccagcgcc ctttgaaggg
      481 cctccgcggc agccggtcta ctaattgccg ctgcgcccca cgttcccaca aactgcgcag
      541 gttggagaga tacgcccccg ccgtctttgg atccaggata atcgccgcca ccgtctcgcc
      601 gtccaaaaag acggccggtg gctgggtgcc ggcgatgacg tccgccccgc tgcggtcgac
      661 ggcaagccgt ccgttgacca gaacgattcg ctccgcggtt tccggcagca cgttgacgct
      721 cccggtcacc tgttcggcgg ctgtctcggc caaaaggtcc cggcaagaga acccgaccgt
      781 cgtccccgga aaggccgcct cgattttctc ccacaaaagc cggatgcccg accgcaaaag
      841 ccacaccggc cggttgaagg tcaccgggaa aaagttggcg taggtgtcgt cttcgaagat
      901 gccgaggaaa gttttcatag gttatatatc ggtctatctg ttaactctcc cggcaactgc
      961 agccacgcgc tcttaagcct gagagaaact gaaggcctga gcgtggccgg tcgttgccct
     1021 tccacttttg accccagccc aagaccctga attgtcgatt tatggcggga atttgcggga
     1081 ataaaccgtc ggtttggcgt cccggtgaaa cttttagcct caccggccga actccacttt
     1141 cggaaccgcg cgcgcagccg gtccttctgg ctggaaatag tcgagtccgg caaaagtgaa
     1201 cattccggcc acgaggtttg aggggaaagt ctgccgggcc gtgttgtact ggaagcagat
     1261 ctcgttgtac gcctttcgcg ccgcggcgat gcgggtttcg atttcggtca actgctgctg
     1321 gagttctcgg aaattctggc tggccaaaag cgcgggataa ttctctgcca cggcaaaaag
     1381 gctcttcagc gttcccgcca aaaactccga cgcctgggcc ttttccggga tcgtcttggc
     1441 ctcctgccac cggctgcggg ccaaagcgac gttctcgaac accccccgct cgtgtccggc
     1501 gtaccctcgc acggtttcgg ccaagttggg gacgagatcg gcccgccgct gcagttccac
     1561 atccacttgc gaccaggcgg cctcgcttcc cgttcgcaag cggacgagcc ggttgtagta
     1621 gacaatgaac agtaccaccg gcgtggcgaa gaggatgacc agaaggaaaa atatcgccgc
     1681 catctacttc gcgcctttgc cctgtttaat aatttcttgg gctttctgca ccatcggccg
     1741 cgcctgcgcg ttgcccttga atttctggac aaactcttcg tagagcggca aaaactgctg
     1801 caaactttcg ggatcctggc tctggacggc aacggccagg ttgaaggcgg ccttatagtg
     1861 caccggtttc aacttcatca cttcccggta ttctttctgc gcttcgccgt acaggccttt
     1921 acccaaatag gcattaccta aattataatg cgcctctacg ttctgcggat cggcggcaat
     1981 cgcctgcttg taggcctcca ccgccttggc tggctgcccc gtttttttgt agacctcggc
     2041 taaatcgagc caggcggtcc cgtccttggg gtttttggc
//
LOCUS       DBNGZA010000247         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2060023, whole genome shotgun sequence.
ACCESSION   DBNGZA010000247 DBNGZA010000000
VERSION     DBNGZA010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2060023"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="AC16FU_05205"
     CDS             <1..1453
                     /locus_tag="AC16FU_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR2993805.1"
                     /translation="DSFHVELENQRKRSKTIVSSIGISSSETFGTPLIINWKRFEKIP
                     LTEFDYENLEVSAKITEADQIQSKGDEVFCVSLDKTSFYFESGGQISDTGKIESEVDP
                     LLMDVVSLQISPSHKKIHVVRPRQGQAGLVKKDIPVKTRINIPRRRAIMRNHTATHLV
                     HKALREVLGEHVHQAGSLVADDRLRFDFTHFKQMSPEELKAIEEQVNEKILENLKVWY
                     DTKSIEYAKNVMKAMALFGEKYGDNVRVIGIGGDKENFGQAYSIELCGGTHTKATGEI
                     GLFKIFSESGIAAGVRRIEAVTGLGLYELTRRQGEQIAQLANLAKAMPEQLAQKVTEL
                     LDENRTLQKQLEEFKKKSAGSVAEQLLAEAQSVNHVKVVTKQMEASSRDDLLHLADQF
                     TVLKEPVVSVLAAQVDGNIIFVGTASPAAIGEKGLKAGDIIREVAKIAGGSGGGKPHL
                     AQAGSKDPSKVASALEAVPGIVQKFMQGAKGRA"
     gene            1450..2190
                     /locus_tag="AC16FU_05210"
     CDS             1450..2190
                     /locus_tag="AC16FU_05210"
                     /EC_number="2.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerol geranylgeranyltransferase"
                     /protein_id="HIR2993806.1"
                     /translation="MNVYTRLTEKLKTQKGLFFVLLDPDRQSPDDLAASALLAQEAGA
                     DALLAGSSLLVTAQFNGALRDLKTQVKIPVILFPANAYQVCPHADAILFLSLLSGRNP
                     QYLIGEQVNGAPLVKAFGLEPIPTGYLLIESGKPTSVAYMSGTQPIPADKPDIAKAHA
                     LAAEYFGMKFVYLEAGSGAVSAVPDALIRAVRDYVSVPLIVGGGIRSPEVARQKVVAG
                     ASAVVVGHALEQRTAPKILREFADAIHG"
     gene            2199..>2746
                     /locus_tag="AC16FU_05215"
     CDS             2199..>2746
                     /locus_tag="AC16FU_05215"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR2993807.1"
                     /translation="MTTAQRLAAKSLRASITVKLDLLEHHLPRIVSLGNRLTHCLKRG
                     RKILICGNGGSAADAQHFAAELVVRLKSKRRALPCLALTTDTSTLTACGNDFGFEEIF
                     ARQVEALGARGDLLVLLSTSGRSANILRAARSAKKRGLVTVALTGSRRNPLTRTAAFA
                     LQVPSTDTQRIQEAHITILHLWG"
BASE COUNT          652 a          739 c          773 g          582 t
ORIGIN      
        1 ggatagtttt cacgtagaat tggaaaatca aaggaaaaga tcgaaaacaa tagtaagctc
       61 aattggtatt tcttcatcag agacgttcgg cacaccgcta attatcaact ggaagcgatt
      121 tgaaaaaatt cctcttacgg agttcgacta cgaaaatttg gaggtttcgg cgaagatcac
      181 cgaagccgat cagattcaaa gtaagggaga tgaggtattc tgtgtttctc ttgataagac
      241 ctctttttat tttgaatcag gtggacaaat cagcgatacc ggtaagattg agagtgaagt
      301 agatccactt ttgatggatg ttgtaagtct ccaaattagt ccttcccata aaaaaatcca
      361 tgttgttaga cctcggcagg gacaggcagg tcttgttaag aaagatattc cggttaagac
      421 gagaatcaac atcccccgcc ggcgagccat tatgcgcaac cacacggcca cccatttagt
      481 ccacaaggcc ctgcgcgagg ttttaggcga gcatgtgcac caggccgggt cactggtggc
      541 tgatgaccgc ttgcggttcg acttcacaca cttcaagcag atgagtcctg aagaactcaa
      601 agctatcgaa gaacaggtta acgaaaaaat tctggagaac cttaaagtct ggtatgatac
      661 caagtccatt gagtatgcaa aaaatgtgat gaaagcgatg gcactttttg gtgagaagta
      721 tggagacaac gtgcgggtaa ttggcatcgg tggtgacaaa gaaaattttg gtcaggctta
      781 ctctattgaa ctctgcgggg ggacgcatac gaaggccacg ggcgaaatcg ggctgttcaa
      841 aatcttttct gaaagcggca ttgcggcggg ggtgcgtcgg attgaagcag ttaccggatt
      901 ggggctctat gaattgaccc gcaggcaggg tgagcaaatt gcccagctgg ccaatctggc
      961 caaagcgatg ccggaacagt tagctcagaa agtgactgaa ctcctcgatg aaaaccgcac
     1021 gctgcaaaag caacttgagg aattcaaaaa gaaatcggcc ggatcggtgg cggaacagct
     1081 cttggccgaa gcgcagtcgg tcaaccacgt caaggtcgtc accaaacaaa tggaggcatc
     1141 cagccgcgac gacctgctcc acctggctga ccagttcact gtgctgaaag agccggtcgt
     1201 cagcgttctg gctgcgcagg tggacggcaa tattatattc gtcgggacgg cgtcaccggc
     1261 ggcgatcggc gaaaagggac tcaaagccgg agatatcatc cgcgaggtgg cgaaaatcgc
     1321 gggcggctcg ggtggcggca agccgcactt agcccaggcc gggagcaaag acccgtcgaa
     1381 agtagcgagc gcattggaag cagtgccggg aattgttcag aagttcatgc agggagcaaa
     1441 aggacgcgcg tgaacgttta cacgcggctg acagaaaaac tcaaaaccca gaagggactc
     1501 ttcttcgtcc tgctcgatcc cgaccgccag tcgccggatg atttggccgc ttccgcgctt
     1561 ttggcgcaag aagcgggtgc ggatgccctt ttggccgggt caagtctttt ggtcaccgcc
     1621 cagttcaacg gcgctctgcg cgatttgaaa acacaggtca aaattccggt gattcttttc
     1681 cccgccaatg cctaccaggt ttgcccgcac gccgatgcga ttctgttcct ctcgcttctc
     1741 tcgggccgca atccgcagta cctcatcggc gagcaggtga acggcgcgcc gctggtaaaa
     1801 gccttcgggc tggagcctat ccccaccgga tatttgttga tagaatcggg caagcccacc
     1861 tccgttgcct atatgagcgg aacccagccg attccggcgg acaaaccgga tattgccaaa
     1921 gcccacgcgc tggcggcgga gtatttcgga atgaaattcg tctacctcga agccggttcc
     1981 ggcgcagttt ccgccgtgcc cgatgcgctc atccgggcgg tacgcgacta tgtctcagtc
     2041 ccgctcatcg tcgggggcgg aattcgctct ccggaagtgg cccggcagaa ggtggttgcc
     2101 ggcgcttctg ccgtggtagt cggtcatgct ctggagcaac gcacggctcc caaaatcctc
     2161 cgcgagtttg cggacgccat tcacgggtag ttccccggat gaccaccgca cagcgtctgg
     2221 cggcaaaatc cctgcgggcg agcattacgg tcaaacttga tttgctcgag catcaccttc
     2281 cccgaatagt ttccttagga aatcggttga cccattgtct caagcgggga cgcaagattc
     2341 tcatctgcgg caacggcggc tcggcggcgg acgcccagca ctttgccgct gaattagtgg
     2401 ttcggctcaa aagcaagagg cgggcgctcc cctgtttggc acttaccacc gacacctcaa
     2461 ccctcaccgc ctgcgggaat gacttcggct ttgaggaaat ttttgcccgg caggtggaag
     2521 cgctgggcgc ccgcggtgac cttctggtgt tactctccac ctccggcagg tcggccaata
     2581 tcctccgtgc cgcccggtcg gcaaaaaagc gcggcttggt gacggtggcc ctcaccggca
     2641 gtcgaagaaa tcctctcacg cggacagccg ctttcgctct ccaagtgccg tccactgata
     2701 ctcagcgcat tcaggaagcc cacatcacca ttctgcacct ctgggg
//
LOCUS       DBNGZA010000248         4298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2589705, whole genome shotgun sequence.
ACCESSION   DBNGZA010000248 DBNGZA010000000
VERSION     DBNGZA010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2589705"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC16FU_05220"
     CDS             complement(<1..526)
                     /locus_tag="AC16FU_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR2993808.1"
                     /translation="MRGHKAGPTISAVVICRNEENVIARCLESLTWADEIIVVDSDST
                     DQTLPVAKKFTSRIFTHPWQGFGPQKQFAVEQAHSDWILVVDADEVVTKELAAEIRLV
                     CTGDTLYQGYRIKRKSNFLGRWVLHGGWYPDFVLRLFRTGKGRFTRARVHESLWVDGS
                     VGTLDGELLHYTDPV"
     gene            complement(549..1658)
                     /locus_tag="AC16FU_05225"
     CDS             complement(549..1658)
                     /locus_tag="AC16FU_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993809.1"
                     /translation="MRILQSIVLDGWSAGSWYMVEFSRQLQKRGHQVVVLTPTGGRTA
                     GKAREAGLEVDTSLDLAHRSPLALVTRSERLRAFLRNGKFDVIQAHWGDDHLAWALAL
                     RGQGSSAPLVRVRAHDPRPPRRHWLARLLHRQLTSGVIVSTDYQRQRYLNGFGLAPEK
                     VVTIRAGIDFADPGLRSLPRNRMSKNFVIGLVGRFSPIKAHGIFFAAARKIAATLPNA
                     QFIVAGYPAEYTLDDVKRLAQANGVADRCEFFGPVENIYEIISRFDLAVISSTGSEAL
                     PRVLLEYLAVGKPVVATAVGAVPEILNQAQCGWLVPPGDALGLARGCLTAYEERERLK
                     TMGARGADFARREFDLDQQVTKTEKFLERLVHGKR"
     gene            complement(1655..2926)
                     /locus_tag="AC16FU_05230"
     CDS             complement(1655..2926)
                     /locus_tag="AC16FU_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR2993810.1"
                     /translation="MKRKRCFLIVFVVLVVGLYGIKVCHKRGDLPVFLQGARNLWTGH
                     QIYYDVPGAFTYPPFFALFVSPFALFPLPVATVLWYLFNVACILGCLLLLRNLILTRD
                     AESKTVNPVLVIGLGFLFTVRFIFDAFENQQFDLVVFCATLLFLLFLSREKPVRSGFF
                     MALAGSLKVTPLLFIVYLLFNRKVNVLAWTFVFLLTLNSVPEIFGLVRGRLYAAEWAE
                     VILKRVNPASGGRPWASGGVWQSSSVFNQSLSAMFYRYFTDVPVEGMKYGSVRVNFAS
                     LDPGRIKLLTYLAYGILFAVTLFVSRPVNSIGGQAQLYEWSLFLLLMVLVSPISSKAH
                     FVVLFLPNLLLWRERLLKKLPAALTVLFFLSFGLTTLTADGLIGRKLADLASAFSCVT
                     LGTLVLWIAVVVRRYQMAGTGRTAGIAPMAI"
     gene            complement(2923..4119)
                     /locus_tag="AC16FU_05235"
     CDS             complement(2923..4119)
                     /locus_tag="AC16FU_05235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2993811.1"
                     /translation="MPVEVKTTAAIARATSSGRRDTGRLPLNVFFLNTIAAEVWGGGE
                     EVFFQWATGLAARGHRVTAAGRLKSLFLDRFKGTDIDIVTLPIGGDFDPRVVWRLSDL
                     FRKKEIDLVCVNFNKGLRLAGLAARWVGRPAVVWVQGVKLPKNRWHHRWLTNRLVDRI
                     IVPSQSLRRELEQFPWLDQRKISVIANGIDLRRFADLTEEGQRVRQQLGVAKSELLIG
                     IFGRLIEAKGHKIFLAAFSELIKKNAGLKTVVVGSGLLESELKNLVTARGLSASVILA
                     GFQKKPGAFMAACDLIVHPALYEPFGLVLLEAMALGKPVVASRVGGIPEVITDGETGL
                     LVPPGDVMALVAAIMQYIENPQLCLEFGARGRRWVEEHFQLDDIMGQVEATLVQIARE
                     RREKVG"
     gene            complement(4109..>4298)
                     /locus_tag="AC16FU_05240"
     CDS             complement(4109..>4298)
                     /locus_tag="AC16FU_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993812.1"
                     /translation="IARLVTGRTTFLIAHRLSSVLRADRIIVLEHGRIVEAGSHSDLL
                     ARGGKYHRLYEMQFSDAR"
BASE COUNT         1064 a         1274 c         1159 g          801 t
ORIGIN      
        1 gaaccggatc cgtatagtga agcagttctc cgtcgagcgt accgacggaa ccatctaccc
       61 aaagcgactc gtgcacccgc gcccgcgtga aacgaccctt gcctgtgcga aagaggcgaa
      121 gcacgaagtc ggggtaccag ccaccgtgca gaacccagcg tccgagaaag tttgatttcc
      181 gctttatgcg gtagccctgg tacagagtat ccccggtgca gaccaaacga atttctgccg
      241 ctaattcttt cgtcaccacc tcgtcagcat caacgaccag aatccaatcg ctgtgcgcct
      301 gttcaacggc aaactgcttt tgcgggccaa agccctgcca cggatgagtg aaaatgcggg
      361 aggtaaattt ctttgccacc ggtagggtct gatcggtgct gtcggaatcg acgacgatga
      421 tttcgtcggc ccaggtaaga ctctccaggc accgggcaat cacattttcc tcattacggc
      481 agatgactac cgcggagatg gtcggaccgg ctttatgccc acgcattgct ttggtggctg
      541 gaaccgattt agcgctttcc atgaacgagc cgctccagga atttttcggt tttcgtcact
      601 tgctggtcaa ggtcaaattc tcgccgggca aaatcggctc cccgagcgcc cattgtcttc
      661 agacgttctc tctcttcata ggcggtcaag cagccacgcg ccaggcctaa ggcatcaccg
      721 ggcgggacca gccagccgca ttgggcctga ttgagaattt ccggaaccgc ccccaccgct
      781 gtcgccacta ccggcttccc caccgccagg tactccaaga gcacccgtgg cagggcctct
      841 gagccggtcg aggaaataac ggcgagatcg aatcgcgaaa taatctcgta aatgttctct
      901 accggcccaa agaattcaca ccgatctgcg acaccattcg cctgtgccag tcgcttaaca
      961 tcatccagcg tgtactccgc agggtagccg gccacgatga attgagcatt gggcagggtc
     1021 gcagcgattt tccgagcagc ggcaaagaag attccatgcg ccttgatcgg agagaaacga
     1081 ccgaccaatc cgatgacaaa attcttactc atccggttcc gggggagtga ccgtaatccc
     1141 gggtcggcga aatcgattcc tgcgcgaatg gtgactactt tttccggtgc gagtccaaac
     1201 ccattcaggt aacgctggcg ctggtagtca gtggaaacaa tgacgcctga ggtaagttgg
     1261 cgatggagca accgcgccag ccagtgccgg cggggcggcc ggggatcgtg cgcccgcacg
     1321 cgcaccaggg gggcgctgga gccctgtccc ctcagagcca gagcccaagc caaatggtcg
     1381 tcaccccagt gtgcttgaat gacgtcgaac ttgccgttcc ggagaaaagc acgtaaccgt
     1441 tccgaccgcg tcaccagcgc cagaggcgat cggtgcgcca gatcgagcga agtatcaact
     1501 tccaaaccgg cttctctggc ttttccggcg gtgcggcctc cggtgggagt gagaaccacc
     1561 acctggtgtc cgcgcttttg cagctgacgg gaaaattcca ccatatacca ggagccggcg
     1621 ctccagccgt ctaagacgat tgattgcaga attctcatat ggccatcggg gcaatgccgg
     1681 ccgtacgccc ggtgcccgcc atttggtacc gacggaccac tacggcgatc cagagcacga
     1741 gagtcccgag cgtgacacag gagaaagcgc tggcgagatc ggccagtttt ctcccgatca
     1801 gtccgtcggc agtcaacgta gtaagtccga aagaaagaaa aaagagtacg gtcaaggccg
     1861 ccggtaattt cttgagcagc cgctcccgcc agaggagcag attcggcaag aacagcacca
     1921 caaagtgcgc cttgctggat atcggggaga ccagcaccat taaaagcaaa aacaaactcc
     1981 attcatacag ttgcgcttga ccaccgattg aattcaccgg ccgcgaaacg aaaagggtta
     2041 ccgcgaacaa aataccataa gcaagatagg tcaaaagttt tatccgtccc gggtcgagag
     2101 aggcaaagtt gactctgacc gacccgtatt tcatcccctc caccggcaca tcagtgaaat
     2161 atcggtagaa catggccgaa agcgattgat tgaacaccga acttgactgc cagacaccgc
     2221 cggaggccca gggtctcccg ccggaagcag gattcacccg cttcaatatt acctccgccc
     2281 attcggcagc gtagagccgg ccgcgcacca gaccgaaaat ttccggcacc gaattcagtg
     2341 tcaggaggaa aacaaaggtc caggccaaaa cgttcacttt ccgattgaaa agcaggtaga
     2401 cgataaaaag gagcggcgtg actttcagtg aaccggccag cgccatgaaa aatccagacc
     2461 ggaccggctt ctcacgagag aggaacaata aaaacagaag ggttgcgcag aagacgacaa
     2521 ggtcgaactg ctgattctcg aaagcgtcga aaataaaacg gacggtgaag agaaacccca
     2581 gacctatgac cagaacggga ttgaccgtct tgctttcggc atctcgtgtc agaatcaggt
     2641 tgcggagcaa aaggagacaa cccaagatac aggcgacatt gaacaggtac caaagcaccg
     2701 tggcgaccgg caacgggaaa agcgcaaaag gtgaaacaaa aagggcaaag aatggcgggt
     2761 aggtgaaagc acccggcacg tcatagtaaa tctggtgtcc cgtccagagg tttcgggcgc
     2821 cctgcaaaaa gacgggaaga tcgccccgct tgtggcagac cttgattcca tagaggccta
     2881 ctacaaggac gacgaagaca atcaaaaaac agcgttttct cttcatccga ccttttcccg
     2941 ccgctcccgc gcgatctgca cgagcgtcgc ctcaacttgc cccataatgt catccaattg
     3001 aaaatgctcc tccacccatc ttcttccccg ggctccgaac tcaaggcaca gttgaggatt
     3061 ttcgatgtat tgcattatcg ctgcgacgag agccatcaca tccccgggcg gcacgagcaa
     3121 acccgtttcg ccgtcggtga tcacctcagg aatgccgccc acccggctcg ccaccaccgg
     3181 cttgcccaag gccatcgctt cgagcagaac caacccaaaa ggttcgtaga gagcaggatg
     3241 aacaataagg tcacaagcag ccatgaacgc tccgggtttt ttttgaaaac cggccaaaat
     3301 tacgctggcc gaaagccccc tcgctgtcac caggtttttt aactcagact cgagtagccc
     3361 ggaaccgact acaaccgttt tgagtccggc gttttttttg atgagctcac tgaacgccgc
     3421 caggaaaatc ttatgcccct tggcttcaat cagccgaccg aaaatgccga tgagcaactc
     3481 acttttggct actcccagct gctgtcgcac cctttgccct tcttcagtga ggtcagcaaa
     3541 tcggcggaga tcaatcccgt tggcaatcac ggagattttt cgctggtcaa gccaggggaa
     3601 ctgttcgagc tccctgcgca aggattgcga tggcacaatg atgcggtcca ccaaacgatt
     3661 cgtcagccag cgatgatgcc agcggttctt gggtaacttt accccttgca cccagaccac
     3721 cgccgggcgg ccaacccacc gcgctgccag cccggccagg cgcagacctt tgttgaagtt
     3781 gacgcagacc agatcaattt ccttcttgcg gaagagatcg gagagcctcc agacgactcg
     3841 cggatcaaaa tcaccgccaa tcggaagagt aacgatatcg atgtccgtgc ctttgaaccg
     3901 gtcgagaaaa agacttttca gacggccggc ggcggtaacg cgatggccgc gcgccgccaa
     3961 gccggtggcc cactggaaaa aaacctcttc cccaccgccc cagacttccg cggcgatggt
     4021 gttcaaaaaa aatacgttca gcggcaatcg gccggtgtcc cggcgtccgg aggaagtagc
     4081 ccgcgcgatg gcagcggtcg ttttgacctc aacgggcatc gctgaactgc atttcgtaca
     4141 gccggtggta cttaccgccc cgggccagaa ggtcggagtg cgagccggcc tcgacaatgc
     4201 gcccgtgttc caagacaatg atgcggtcgg cgcgcagaac cgacgacagc cggtgagcaa
     4261 tcaaaaatgt ggttcgaccg gtgaccaggc gggcaatc
//
LOCUS       DBNGZA010000249         1242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_893958, whole genome shotgun sequence.
ACCESSION   DBNGZA010000249 DBNGZA010000000
VERSION     DBNGZA010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_893958"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16FU_05245"
     CDS             <1..638
                     /locus_tag="AC16FU_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2993813.1"
                     /translation="VLGGAMTAFWLNVLWSMASQFYWEKQGGNLDLYLVAPISRMAIL
                     LGMAIGGFFMTFSRAAAVVIGGVILFHVGFTVQSYGKLFLVFGLSLVALYGLGMLFSS
                     LFLLFGREAWHIVTMFQEPVYLASGFYFPVRALGFYVALAASIVPVTLGMDAMRQLLF
                     PTGRIHGLLPVNTEMLLLSGLSIFFLFLAQRALGYMEKLGKREGRLTLRWE"
     gene            702..>1242
                     /locus_tag="AC16FU_05250"
     CDS             702..>1242
                     /locus_tag="AC16FU_05250"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR2993814.1"
                     /translation="MNLKREHQKLLAEFRRQKDSKYAKNQAVFVPTKYQILGVRVPIM
                     RQIAGTWAKRHGDISPQGLFAFTDLLWKGRTLEELVFATLLLWRFRKFIPSLTQKHFD
                     SWRRKLDNWGSTDGLGVTVLGPWVLADTQRRIPYLKRLIGNRNLWSRRLALNGAIALI
                     RSQRDGKATKVAFEFIDRVK"
BASE COUNT          259 a          329 c          342 g          312 t
ORIGIN      
        1 tagttttagg cggggcgatg accgctttct ggcttaacgt cctctggtcg atggcttctc
       61 agttctactg ggaaaagcag ggggggaatc tggacctcta tctggtcgcc cccatttcgc
      121 ggatggcgat tcttctcggg atggccatcg ggggattttt tatgaccttc tcgcgggcgg
      181 ctgcggtggt catcggcggg gtcatcctgt tccatgtcgg tttcaccgtg cagagttacg
      241 gaaaactctt tctggtcttc gggctttcgc tggtcgcact gtacggattg gggatgctct
      301 tttcgtcgtt atttttgctc tttggccggg aggcctggca tatagtgact atgtttcagg
      361 aaccggtcta cctcgcttca gggttctact tccccgtgcg ggcgctgggt ttctacgtcg
      421 ctttggcggc atcgattgtg ccggtgacct taggcatgga tgcgatgcgg caactcttgt
      481 tccctacggg acggatacac gggctcttgc cggtgaacac cgaaatgctc cttctctcag
      541 gtttatctat cttctttctt ttccttgccc agcgtgctct gggttatatg gaaaaattgg
      601 gcaagcggga ggggcggctg accttgcgct gggagtagct gtcaacggat tgcgaacgga
      661 ttaaacactc gactcacact tcccgttaag cacgccagag aatgaatctc aaacgagaac
      721 accaaaaact tctggcagaa ttcaggcggc aaaaagatag taagtatgcc aaaaaccaag
      781 ctgttttcgt tcccacgaaa taccaaattt taggcgtccg ggtgcccata atgagacaaa
      841 tagccggaac gtgggccaag aggcacggag acatttctcc gcagggactt ttcgccttta
      901 ccgatctcct ctggaaaggg agaacgcttg aagaattggt ctttgcgacg ttgctcttat
      961 ggcgcttccg gaagttcatt ccctccttaa cccaaaagca ctttgactct tggcgcagaa
     1021 aactggacaa ctggggctcc accgacggat tgggggtgac tgtcctgggc ccctgggtgc
     1081 tggcagatac ccaaagacga atcccctacc tcaaacggct catcggcaac cgcaacctct
     1141 ggagccggcg tctcgcccta aacggagcca tagcgctaat tcgaagccag cgtgacggaa
     1201 aagcaaccaa agtcgccttc gaattcatcg accgcgtcaa gc
//
LOCUS       DBNGZA010000250         4763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2696074, whole genome shotgun sequence.
ACCESSION   DBNGZA010000250 DBNGZA010000000
VERSION     DBNGZA010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2696074"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="cysS"
                     /locus_tag="AC16FU_05255"
     CDS             complement(<1..1178)
                     /gene="cysS"
                     /locus_tag="AC16FU_05255"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR2993815.1"
                     /translation="MALVFYNTLSRRKEEFVPISSGQVKIYTCGPTVYDFAHIGNFRT
                     NVFQDLLRRYLEYKGLRVTQVMNLTDIDDRIIAGVRSKKLPLREYTEPYKQAFFADLD
                     TLGIKRADFYPEATAHVPEMAALVERLLEKGNAYRQDSSIYFKIGSFPAYGGLSHMKL
                     DELKAGARVSADSYEKEQVSDFALWKAWDEQDGDIFWETPLGKGRPGWHLECSVMSMK
                     YLGEHFDIHTGGVDLIFPHHENEIAQSEAATGKKFVNYWLHAEHLIVEGRKMSKSLGN
                     FYTLRDILAKGYSPKAVRYLLLSVHYRQQLNFTFDGLEAARNAVERLLDFKRNVADAK
                     EAAENPKVAEQVAQTKLGFEENLDDDLNISGALGVLFDFVRDMNKIIADRGLSAKDKT
                     "
     gene            complement(1307..2383)
                     /locus_tag="AC16FU_05260"
     CDS             complement(1307..2383)
                     /locus_tag="AC16FU_05260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR2993816.1"
                     /translation="MDFSETVLRELTDAPGVPGKEQAVARVMEKYLKPVAEISYDRLG
                     SVIAKKKGTGDKPRVLVAGHMDEVGFVVSEITKNGYIKFIPLGGWWGPVVLAQRMKIY
                     TQNGSAVVGVTGAKPPHLLSEEDRKKLPEIKDMFIDVGVTDRFDVQKKLGIRPGDAIV
                     PDSQFTVLANERVYLNKAFDNRFGCAAVVELLTRLKGKKHPNTVYGAGTVQEEVGLRG
                     AGTTAYAVEPDVAIAADVGIARDTPGIDGDHPEKMGSGVSIFVHDSGMIPNWKLVDLV
                     IDTAKKNKVPYHMAALERGTTDGARVQVSRTGVPTVVLGVATRYIHAHSAMLSRDDYD
                     NYLKLLVALVMRLDQKTVAGLTRA"
     gene            complement(2416..3162)
                     /locus_tag="AC16FU_05265"
     CDS             complement(2416..3162)
                     /locus_tag="AC16FU_05265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2993817.1"
                     /translation="MSAVKVVLFDLGSTLLEYENVPWETLDRRCIASGYRYLAEAGRP
                     LPPAEQFAAVFFDRFRAMWRETERTLAEIDLARFAEEVLQSLGITWRDGEKAGFFERY
                     YRPIFEQITPYPESAAVLESLSRADLRLGLVSNSIFPAQFHRQELQKFGLARFFDVTI
                     FSSEFGWRKPKPEIFQTALEQLRALPQEAIFVGDRVREDIWGPQQVGMRAVLKWRADR
                     EWSAGTVPDAIIHSLVELPAVLEVLNRTKV"
     gene            complement(3149..4201)
                     /locus_tag="AC16FU_05270"
     CDS             complement(3149..4201)
                     /locus_tag="AC16FU_05270"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR2993818.1"
                     /translation="MKILVLLGGDSAEREVSLASGEAVVKALQKQGHQVIAADPAVGL
                     KRLSDTNSILLGRIKKEPPAPEKLPVATEKTVIQTVESFDFEESDVIFLALHGGKGED
                     GTLQALLDMTGKPYTGSGMLASALAMNKAVSKRLFVQARIPTPHWLLCDTKRSRNLDQ
                     IVEEIRAGFAFPLVVKPHNQGSTVGLSVVHAPSELKPALETAAKFSPEVIVEEFIAGR
                     EITVGILGERALPVVEIIPQHEVYDYECKYTKGRSDYVCPAELTKEEAEEVQNLGLRA
                     FQCLGCSGYGRVDFRMSREGKFYCLEVNTLPGMTALSLVPMAAKGAGLDFPHLLEAII
                     EQALMYKKSGAAVVRS"
     gene            complement(4198..>4763)
                     /locus_tag="AC16FU_05275"
     CDS             complement(4198..>4763)
                     /locus_tag="AC16FU_05275"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2993819.1"
                     /translation="VFLYLSAIVESLFPPYPGDVVTFAGGYLASLGKLNFPLVFLATS
                     LGSLSGLLLLYAIGQRHGRRLFQKEQSHWRTPAHLAKVERWFVRYGAKILIASRFLSG
                     IRSGIALAAGIGNIGWRRMVLYGTVSILLWNGLILFVSSFVEQNWQRLYGLLLLYNKV
                     LFGGLLLTAIGFGIWWWRKRQVPKGAA"
BASE COUNT          992 a         1508 c         1242 g         1021 t
ORIGIN      
        1 tgcgttttgt ctttcgcgga aagacctcgg tcggcgatta ttttattcat gtcccgcacg
       61 aaatcgaaca gcacccccag tgccccggaa atgttcaaat cgtcatcgag attctcctcg
      121 aatcctagct tggtctgggc tacctgttca gcaacttttg gattttctgc cgcttctttt
      181 gcatcagcca cgttgcgttt gaagtctaag agccgctcca ccgcattccg cgccgcttcc
      241 aggccgtcga aggtgaaatt taactgctgc cggtaatgga cggagagcaa aaggtaccgc
      301 accgctttgg gagagtaccc cttggccagg atgtcgcgca gggtataaaa gttgcccagc
      361 gacttcgaca tcttccggcc ttcaacgatc aggtgttcgg cgtgcagcca gtagttgacg
      421 aacttcttgc ccgtcgccgc ctcgctctgc gcgatctcgt tctcgtggtg ggggaaaatc
      481 aaatcaaccc cgccggtgtg aatgtcgaag tgctcgccca gatacttcat gctcatcacc
      541 gagcattcca gatgccaccc tgggcgcccc ttgcccaatg gcgtttccca gaagatatcg
      601 ccgtcctgtt cgtcccaggc cttccagagg gcaaagtcgg agacctgttc tttttcgtat
      661 gaatcggcgc tgacgcgggc cccggctttc agttcgtcca acttcatgtg cgagagcccg
      721 ccgtaggcgg ggaacgagcc gattttgaaa tagatcgagc tgtcctgtcg gtaggcgttc
      781 cctttctcca aaagtcgttc gaccaaagcc gccatctccg gcacatgcgc ggtggcctcc
      841 gggtaaaagt cggcccgctt gatgcccagg gtatccaagt cggcgaaaaa ggcctgcttg
      901 tacggctccg tgtactcccg cagcggcagt ttttttgacc gcaccccggc gatgatccga
      961 tcgtcgatgt cggtcaggtt catcacctgg gtgacgcgca agcccttgta ctctaaatag
     1021 cgccgcagga ggtcctggaa gacgttggtg cgaaaattcc cgatgtgggc aaagtcgtac
     1081 accgtcggcc cgcaggtgta gatttttacc tggcccgaac tgatcggaac gaactcctcc
     1141 ttccggcggg ataaagtatt gtagaaaacc aaagccattg tgcccttaat aaagtagccg
     1201 ggctcgtctc cgagcccggc ttttaccctg gaccggctcc gggacgagcc ggtctaccag
     1261 tgaaattgag agagcgatcg ttcgaaccaa agccaccccg gagatcctac gctctggtca
     1321 aacccgctac cgtcttctga tccagccgca tcacgagcgc cactagaagc tttaggtagt
     1381 tgtcgtagtc atcccgcgag agcatcgcgc tgtgggcgtg gatgtaacgc gtcgccacgc
     1441 ccaaaacgac cgtcggcacg ccggtgcggg agacctgaac ccgggcgccg tcggtggtgc
     1501 cccgctccaa agccgccatg tggtacggca ctttgttctt tttggcggtg tcgatgacga
     1561 gatcgactaa cttccagttc ggaatcatcc cgctgtcgtg gacgaaaata gagacgccgg
     1621 agcccatttt ttccgggtga tcgccgtcga tccccggcgt atcgcgggca atccctacgt
     1681 cggcggcaat ggccacgtcc ggctccaccg cgtaggcggt ggtgcctgcc ccgcgtaaac
     1741 cgacctcctc ctgcaccgtc cccgcaccat agaccgtgtt cgggtgcttt ttgcctttca
     1801 ggcgggtcaa gagctccacc accgccgcac agccgaaccg gttatcgaag gccttgttca
     1861 ggtagacccg ctcgttcgcc agaacggtga actgactatc cgggacgatg gcatccccgg
     1921 gccggatgcc caatttcttt tgcacgtcaa aacggtcggt cacccccacg tcgataaaca
     1981 tatcctttat ttccggcagt ttcttgcggt cttcctcgga gaggaggtgc ggcggcttgg
     2041 ccccggtcac ccccaccacc gccgacccgt tctgggtgta gattttcatc cgctgcgcca
     2101 gcaccaccgg cccccaccag ccacctaacg ggatgaactt gatgtaaccg tttttggtga
     2161 tttccgacac gacaaaaccc acttcgtcca tgtgcccggc gactaaaact ctgggcttgt
     2221 cgcccgtgcc tttctttttg gcaataacgc tccccagccg gtcgtacgaa atttcagcca
     2281 ccggtttgag gtatttctcc atcacccggg ccaccgcctg ttccttgccc ggcacgcccg
     2341 gcgcgtcggt caattcccgt aaaaccgtct cgctgaaatc catcttcgct cctctatctc
     2401 tgctttttac gattcttaca ctttcgttcg attcaacact tccaaaaccg ccggtagttc
     2461 gaccagggaa tggataatgg catccggcac cgtgccagcc gaccactcgc ggtcggcccg
     2521 ccatttgagc accgcgcgca tcccgacctg ctgcggcccc cagatgtctt cccggacccg
     2581 gtctcctaca aagatcgctt cctgtgggag cgcgcgcaac tgctccagag cggtttggaa
     2641 aatttccggc ttgggcttgc gccagccgaa ttccgaagag aaaatcgtaa cgtcaaagaa
     2701 acgagccagc ccgaacttct gtaattcctg ccggtggaac tgggccggga aaatcgagtt
     2761 cgacaccagc cccagccgca ggtctgctcg ggagagcgac tccagaactg cggcactttc
     2821 cgggtagggc gtgatttgct caaaaatcgg ccggtagtac cgctcgaaga aacccgcctt
     2881 ctccccatcg cgccaagtga tccccaaact ctgcaaaact tcttccgcaa agcgggctaa
     2941 gtcgatttcc gccagcgtcc tttcggtttc ccgccacatc gcgcgaaaac ggtcaaagaa
     3001 aacggcggca aactgctccg ccggaggcag cgggcggccg gcctcggcca aataccgata
     3061 cccggaagca atacaccgcc gatctaaggt ttcccaaggg acattttcat actccaagag
     3121 cgtggagccc aaatcgaaaa gaaccacttt aactgcggac aacggctgct ccactttttt
     3181 tatacatcag cgcctgctcg attatcgcct ccaaaaggtg cggaaagtcg agcccggccc
     3241 ccttggccgc catcggcact aaggaaagcg cggtcatacc cggcaaggtg ttgacctcca
     3301 agcagtagaa cttcccttcc cggctcatcc ggaaatcgac ccgtccgtag ccggagcagc
     3361 ccaggcattg aaaggcccgc agtcccaggt tttgaacttc ctcggcctct tccttcgtca
     3421 actccgccgg gcaaacgtaa tccgaacgtc cctttgtata cttgcactcg taatcgtaca
     3481 cttcgtgctg gggaatgatc tccaccactg ggagcgcccg ctcgcccagg atgcccacgg
     3541 tgatttcccg gccggcaatg aattcttcca caataacctc tggagaaaac ttcgccgccg
     3601 tctccaaggc cggcttgagt tcgcttggcg catggacgac cgaaagccct accgtcgagc
     3661 cctggttgtg cggcttgacc accagaggga atgcgaagcc cgcccggatt tcttccacaa
     3721 tttggtcgag attacggctt cgtttcgtat cgcacagcaa ccagtgcgga gtgggaattc
     3781 tggcttgtac aaaaagacgc ttggaaaccg ccttgttcat cgccagggca ctggccagca
     3841 tcccggaacc ggtgtagggc ttgccggtca tatccaaaag cgcctggagc gtgccgtctt
     3901 cccctttccc tccatgcaga gccaaaaaga tgacgtcgct ttcctcgaag tcgaacgatt
     3961 cgacggtttg aattaccgtc ttttcggtgg ccaccggcaa cttctccggc gccggcggct
     4021 cttttttgat tctgcccaaa agaattgagt tggtgtccga aagccgcttc aatcccaccg
     4081 ccgggtcggc ggcaatcacc tggtgcccct gcttctgcag ggctttcacc accgcttcac
     4141 ccgaggccag ggagacttcc cgctccgccg aatcgccgcc caataagact aaaatcttca
     4201 cgccgctcct ttcggtactt gcctcttccg ccaccaccag atgccgaagc ctatcgcggt
     4261 cagtagaagg ccgccaaaca ggaccttatt gtagagcaaa agcaggccgt agagcctttg
     4321 ccagttctgc tccacaaagg aagatacaaa aagaatcagg ccgttccata atagaatcga
     4381 gaccgttcca tagaggacca tcctccgcca gccgatgttc ccgatccccg ccgctaaggc
     4441 gatgccggag cggatgccgc ttaagaaccg gctggcaatg agtatcttgg ctccgtaccg
     4501 gacaaaccag cgctcgactt tggctaaatg agccggcgtc cgccaatggc tttgctcctt
     4561 ttgaaagagt cggcgtccgt gcctttgacc tatagcgtac aggagcagaa gcccggagag
     4621 actccccaga ctggtggcca aaaagactag ggggaagttc aattttccta gtgacgctaa
     4681 gtatccgccc gcaaaagtca ccacgtctcc cggatagggc ggaaaaagcg actcgacgat
     4741 ggcgctcaaa tacaaaaata cgt
//
LOCUS       DBNGZA010000251         5668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2590277, whole genome shotgun sequence.
ACCESSION   DBNGZA010000251 DBNGZA010000000
VERSION     DBNGZA010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2590277"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="AC16FU_05280"
     CDS             complement(<1..836)
                     /locus_tag="AC16FU_05280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2993820.1"
                     /translation="MTGSTPATSPASYKRVKGIFGPDRVLSDPGQLETYSSDATKLTY
                     LPDLVVFPANADELQKLIRVAMAEKIPLVPRGAGTGYTGGALAVKGGIVISFEKMNHI
                     LGINAERRIVFTEPGVITYEIAQAAQKAGLFYPPDPASIKISTIGGNVAECAGGLRCK
                     KYGVTKQYVLGLEFVAGTGEIYRTGVLTENGGLGFDFTALLTGSEGTLGFITRIALKL
                     INPPEARRTFLATFASTEKAAAVVAEFTRQGVVPSLVEFVDQDALAITLTYLHKSGFT
                     DAA"
     gene            complement(838..1329)
                     /locus_tag="AC16FU_05285"
     CDS             complement(838..1329)
                     /locus_tag="AC16FU_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR2993821.1"
                     /translation="MSHTVLLVDGYNLIGQTNALKAQANLAVGRERLIERLSDLAFQG
                     NYKVLLVFDAQYQGQAWVQKARERQVTVLFTQKGQTADQFIVTWLKKRPHRGGVIVIT
                     RDRELVAVLRKLRVRILPDLSSLEAAIADGAGLISHQSQPHLVGEQLSAKSFEALRGV
                     VRK"
     gene            complement(1331..2456)
                     /gene="prfB"
                     /locus_tag="AC16FU_05290"
     CDS             complement(join(1331..2380,2382..2456))
                     /gene="prfB"
                     /locus_tag="AC16FU_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157471875.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR2993822.1"
                     /translation="MPVATTTITNLDELKERLHKLGNYLDLPGKRKQVTDLEVQTAAP
                     DFWNNGEKAQAILKEITATKRILERWEGLFQRVEEAALFRQMAAEENDPAAENEAEAQ
                     AVQLAGEIAAFELESLLSGPDDGKNAIVTIHAGAGGTEAQDWASMLLRMYLRYFERRG
                     FKAEMFDYADGEEAGIKSATVEVTGLYAYGYLKAESGVHRLVRISPFDANKRRHTSFA
                     SVFIYPEIEDNIEIDIKDEEIRVDTYRASGAGGQHVNKTSSAVRITHLPTGIVVQCQT
                     ERSQHRNRESALKVLRARLYQFHKEKEEEKFLAIEKSKKKIEWGSQIRSYVFHPYTLV
                     KDHRTEHETSNVQAVMDGEIDDFIQAFLAHKNETPLVTGA"
     gene            complement(2558..3784)
                     /gene="tyrS"
                     /locus_tag="AC16FU_05295"
     CDS             complement(2558..3784)
                     /gene="tyrS"
                     /locus_tag="AC16FU_05295"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR2993823.1"
                     /translation="MKFPSIEEQLAVIKQGAVEVIPETELVTKLKRAQATGKPLRVKQ
                     GFDPTAPDIHLGHTVGLRKLRQFQELGHRIVLIIGDYTGMVGDPSGRSATRPQLSFGA
                     VRRNAGTYLKQFAKVLDREKVEIHYNGEWFSKMSFQEIMALAGKFTLARMLERDDFSI
                     RYKEQSPIAIHELFYPLMQAYDSVAIRADVEIGATEQKFNLLAGRQIQEEYGQEPQIV
                     LTLPVLVGTDGVQRMSKTTGNYIGVAEPPDQVYGKAMSIPDEQILPYLELVTDCSPTE
                     LTQAREELRKRSVNPMLWKKKLAHRLVTLYHGKGAADRAQAEFEQVFSEKKLPEQIPG
                     VALSYRNGKIPIVQLLMETKQADSKSAARRLIQQGGVSVNQKRVADLDFEVELLGEVI
                     VKVGKRKFIRVLPKRR"
     gene            3881..>5668
                     /locus_tag="AC16FU_05300"
     CDS             3881..>5668
                     /locus_tag="AC16FU_05300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR2993824.1"
                     /translation="MTLFIRYMDESKFAVEKYPATAPYPLPPRRRRRVSVFLGYLLGS
                     LTLAGLVAALLTYRVYKHDLPSLAQLTNLQPSLATKVYARTGRVLKEFYTERRIFVPL
                     GQMPPYLIDALLATEDRKFYNHWGVDPLGVARALFSNLSRGELVGQGGSTVTQQLART
                     LFLTRQKTLSRKIKEILTAIKIERAYSKREILELYLNQCYFGKGAYGIQAAAQTFFSR
                     SAPELTLPQCALLIGLLQAPNRYSPLEHPERTLARRNIVLDAMVDYKKLNANFADSLK
                     RLPLELSPNVDVLGEAPYFTEMVRQYLEDRYGEKMLFSGGLMIQTTVDDSLQKIAEEA
                     VRSVIDSLQKDLERSHTLSDPRYTEEVVDTVNGKIEKRRQYKQIQGAFLAIDNATGDV
                     ITMVGGRDFNQSKFNRAVQALRQPGSAFKPFVYTAAVDNGYRPCDLLFDSPLVLTIPG
                     SGEWRPQTIDFSFRGPMTLREGLQFSRNLIAIKLLQKIQPQQAIYYAKKMGITSPLQP
                     VPSLAIGTSEVSMWDMVSAYSVFPNGGIRIEPKYILRITDRFGNILEKHETSKREEAL
                     SPTTAYIMVNLLQTVVDRGTGARTRAMGFT"
BASE COUNT         1074 a         1753 c         1596 g         1245 t
ORIGIN      
        1 gctgcgtccg tgaagccgga cttgtgcaga tacgtcaggg taatggccag cgcatcctgg
       61 tcgacgaatt caaccagtga aggtaccact ccctgccggg taaactccgc cacaaccgcc
      121 gccgcctttt cggtggaggc aaacgtcgcc agaaaagtcc ggcgcgcttc cggcgggttg
      181 attaacttca gcgcaatccg ggtgatgaac cccagcgttc cttccgagcc ggtcagaagc
      241 gcggtgaagt cgaaaccgag acccccgttc tcggtcagca cgccggttcg ataaatttcg
      301 cccgtacctg ccacaaactc caacccgagg acgtactgct tggtcacccc gtatttctta
      361 cagcgcaacc cgccggcgca ttcggccacg ttcccgccga tggtggagat tttaatcgag
      421 gccgggtcgg ggggataaaa gagcccggct ttttgagcgg cctgcgcaat ttcataggtg
      481 atgacgccgg gttcggtaaa aacaatcctt ctctccgcat tgatgcccag gatgtggttc
      541 attttctcaa aactgatcac aatcccgcct ttgaccgcca gtgccccgcc ggtgtaaccg
      601 gtgcccgccc cgcgcggaac cagcggaatc ttttccgcca tagctactcg gattaacttc
      661 tggagttcgt cagcgttggc cgggaaaaca acgaggtcgg gcaggtaggt taacttggtg
      721 gcgtcgctgg aataggtttc caactgcccc gggtcggaaa gcacccggtc tggtccaaat
      781 atccctttga cacgtttgta ggaggccgga gaagttgcgg gggtagaccc ggtcacttca
      841 ctttcggacg acaccgcgca gggcctcaaa actcttggcg gagagctgct cgcccaccag
      901 gtgcggctgg ctttgatgcg agatcaggcc cgcgccgtcg gcaatggccg cttccagact
      961 ggaaaggtcg ggcaggatgc gcacccgtaa cttgcgcagc acggccacga gttcgcggtc
     1021 gcgggtgatg acaatcaccc cgccccggtg cggccgtttc ttgagccagg tgacgatgaa
     1081 ctggtcggcg gtctgtccct tctgcgtgaa gaggacggtc acctgccgtt cacgcgcctt
     1141 ctgcacccag gcctgtccct ggtactgggc gtcaaaaacc aagagaacct tgtaattgcc
     1201 ctgaaaggcg aggtcggaga ggcgctcaat cagccgctcc cgcccgacgg ccagattggc
     1261 ctgcgctttg agcgcgttcg tttgcccgat caggttgtag ccgtcaacta agagcacggt
     1321 gtgggacatc tcaggctccg gtcaccagcg gcgtttcgtt tttgtgcgcc agaaaggctt
     1381 gaatgaaatc atcaatttcc ccgtccatca ccgcctggac gttggaggtt tcgtgctcgg
     1441 tgcggtggtc tttcaccagc gtgtacggat ggaaaacgta ggagcggatc tggctccccc
     1501 actcgatttt cttcttcgac ttctcgatcg ccaaaaattt ctcctcttcc ttttccttat
     1561 ggaactggta cagccgcgcg cgcagcacct tcagcgcgct ctcgcggttg cggtgctggg
     1621 agcgctcggt ctggcactgc accacgatgc cggtgggcag gtgggtgatg cgcacggccg
     1681 aagaggtttt gttcacatgc tggccgcccg cacccgaggc acggtaggtg tccactcgga
     1741 tctcttcgtc cttgatgtcg atttcaatgt tgtcttcaat ttccgggtag atgaaaactg
     1801 aggcaaacga ggtgtgccgt ctcttgttgg cgtcgaaggg tgaaatccgc accagacggt
     1861 ggacgcccga ttcggccttg aggtagccgt aggcatacag accggtcacc tccaccgtgg
     1921 cgctcttgat tccggcctct tcaccgtcgg cgtagtcgaa catttcggcc ttaaagcccc
     1981 ggcgctcgaa atagcgcagg tacatccgca agagcattga agcccaatcc tgggcctcag
     2041 tgcctcccgc tccggcgtgg atcgtcacga tcgcattctt gccgtcatcc ggcccggaga
     2101 ggagcgactc cagttcaaac gcggcgatct ccccggctaa ttgcaccgcc tgcgcctccg
     2161 cttcgttttc ggcggccggg tcgttctcct ctgccgccat ttggcggaaa agcgcggctt
     2221 cctccaccct ttggaacaaa ccctcccagc gctcgaggat ccgcttggtg gcggtaattt
     2281 ccttcaggat ggcctgggcc ttttccccgt tgttccagaa atcaggtgcg gcggtctgaa
     2341 cctccaagtc ggtcacctgt ttccgcttcc ccggcaggtc aaaggtagtt cccgagttta
     2401 tgcagccgct cttttagttc gtcgaggttc gttatcgtcg ttgtggccac gggcattttc
     2461 tcctcaaggc gtgtcgaagt gtcggggtat cggagtgtcg aggttagata cctttttcga
     2521 ccccgacacc tcgacacgtc gatacctcaa cactttatta cctccgtttc ggcagcacgc
     2581 ggataaattt ccgcttgcct acttttacga taacctcacc caaaagctcc acctcaaaat
     2641 ctaagtctgc cacccgcttc tggttcacgg agactccgcc ctgctggatg agccggcggg
     2701 ccgcgctctt cgagtccgcc tgcttcgttt ccatgagcaa ctggacgatg ggtatcttcc
     2761 cattccgata cgagagagcc acaccgggga tttgttccgg caatttcttt tcagaaaaga
     2821 cttgttcaaa ctcggcctgc gcgcggtcgg cggccccttt cccgtggtac agggtaacca
     2881 gccggtgcgc cagtttcttc ttccagagca tcggattcac cgagcgcttt cttaattcct
     2941 cccgcgcctg ggtcagttca gtcggactgc aatcggtcac caattctaaa tagggtaaaa
     3001 tttgctcgtc gggtatcgac atcgccttgc cgtaaacctg atccggcggt tcagcgacgc
     3061 cgatgtagtt ccccgtggtc ttgctcatcc gctggacgcc gtcggtcccg accaggaccg
     3121 gcagggtgag cacgatctgg ggctcctggc cgtactcctc ctggatttgc cggccggcca
     3181 aaaggttgaa cttctgctcg gtggcgccga tttccacgtc ggctcggatt gccaccgaat
     3241 cgtaggcctg catcagggga tagaacagtt cgtggatggc tatggggctc tgctctttgt
     3301 agcggatcga gaaatcgtcc cgttccagca tccgggccag ggtgaatttg ccggccaggg
     3361 ccatgatttc ctgaaaactc attttgctga accattcgcc gttgtagtga atttcgactt
     3421 tttcccggtc gagtaccttc gcaaactgtt ttaagtacgt cccggcattg cgccgtactg
     3481 cgccaaaaga aagctgcggc cgggtggcgg agcggccgga cggatcgccc accatgccgg
     3541 tgtagtcgcc gatgatgagc acgatccggt ggcccagttc ctggaactgg cgcagcttgc
     3601 gcaatcccac cgtatggccc aggtggatgt cgggagcggt gggatcaaat ccctgcttga
     3661 ctcgcagggg ttttcccgtc gcctgagcac gcttgagttt ggtcaccaat tccgtttccg
     3721 gaatcacctc caccgcgccc tgcttgatga cggctaattg ttcttcaatg gatggaaatt
     3781 tcatccgaca taggataata gtcggaaggg aaaaaggcaa gtgcctgcga cgcatccggc
     3841 acgctgtttc ctgagagaaa accttgacag tcgcctgcaa atgacccttt ttatcaggta
     3901 tatggacgag tcgaagtttg ccgtggaaaa atatccggcg accgcgcctt atcccctgcc
     3961 gccccgccgg cgccgccgcg tatcggtttt tctcgggtac ctcttgggtt ctttgaccct
     4021 ggccgggctg gtagcggcat tgcttaccta ccgggtctac aagcacgatc tcccttcgct
     4081 cgcgcagctg accaacctcc agccctcgct ggcgaccaaa gtctacgccc gtacgggccg
     4141 ggtgctcaag gaattttaca ccgaacgccg gattttcgtc ccgctggggc agatgccgcc
     4201 ctatttgatc gatgcgctct tagccacgga ggaccgaaaa ttctacaacc actggggtgt
     4261 cgatcctttg ggagtggcgc gcgccttgtt ctccaacctt tcccgcggcg aactggtcgg
     4321 ccagggcggc tccaccgtga cccagcagct ggcccgcacc ctctttttga cccgccagaa
     4381 gaccctttcg cggaagatca aagaaatcct gaccgccatc aaaatcgaac gggcctattc
     4441 gaagcgcgag attctcgaac tgtacttgaa tcagtgctac tttggcaaag gcgcttacgg
     4501 cattcaggcc gcggcccaga cctttttcag ccggagcgcg ccggaattga ccctgccgca
     4561 gtgcgcgctg ctcatcggcc tcctgcaggc gccgaaccgc tactcccctc tggagcatcc
     4621 ggagcggacg ctggcgcgcc gcaacatcgt tctggatgcc atggtggatt acaaaaaatt
     4681 gaacgcgaac ttcgccgaca gtctcaaacg cctgcctctg gagctctcgc ccaacgtcga
     4741 tgtgctgggc gaggccccct acttcaccga aatggtccgc cagtacttgg aagaccgcta
     4801 cggcgagaaa atgctcttct caggcggcct catgatccag acgaccgtcg acgattccct
     4861 gcagaaaatt gccgaggagg ccgtgcgctc ggtcatcgat tcgctccaga aagatctgga
     4921 gcggagtcac acgctatccg acccccgcta caccgaagaa gtcgtcgata cggtgaacgg
     4981 caagatcgaa aagcggcgcc agtataaaca gatccagggt gcgtttttgg ccatcgacaa
     5041 cgccaccggc gacgtgatca ctatggtcgg gggccgggat ttcaaccaga gcaagttcaa
     5101 ccgcgcagtg caagccctgc gccagcccgg ttcggccttc aagccgttcg tttacactgc
     5161 ggcggtggac aacggctacc gcccctgcga cctgctcttc gattcgcccc tggtgctcac
     5221 gatccccggc tctggcgagt ggcggccgca gactatcgat ttttccttcc ggggccccat
     5281 gactttgcgg gaagggcttc agttctcgcg gaatttaatc gccatcaaac tcctgcagaa
     5341 aattcagccc cagcaggcga tctactacgc caagaagatg gggatcacct cgcccttgca
     5401 gccggtgccg tcgctcgcca tcggcacctc cgaagtctcg atgtgggaca tggtgtcggc
     5461 ctactcggtt ttcccgaacg gcggcatccg gatcgaaccg aagtacatcc tgcgcatcac
     5521 cgaccggttt ggcaacatct tagagaagca cgagacttcc aagcgggagg aagccctctc
     5581 ccccaccacg gcttacatca tggtaaatct cctgcagacc gtagtggacc gcggcaccgg
     5641 tgcgcggacg cgggcgatgg ggtttacc
//
LOCUS       DBNGZA010000252         4644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1637656, whole genome shotgun sequence.
ACCESSION   DBNGZA010000252 DBNGZA010000000
VERSION     DBNGZA010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1637656"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16FU_05305"
     CDS             complement(<1..208)
                     /locus_tag="AC16FU_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993825.1"
                     /translation="MTEAELSAQTANPKLRNVFPRTSGDFLYMNTPGIKELISVPSKR
                     FRRIARFEFWAYTPLASQRLTVELA"
     gene            117..1013
                     /locus_tag="AC16FU_05310"
     CDS             117..1013
                     /locus_tag="AC16FU_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2993826.1"
                     /translation="MFIYKKSPLVRGNTFLNLGFAVWAESSASVIDTNRFTRTSPGAG
                     LLSLRGLNKSNLLVRGCVFTDPAQNSISVNQSTVTVQACSLAGQAITAVAAENGSTLN
                     IRKSRLANYSAYGLHTNYQSQGDAGKVSNPGENFFGEATPVDSLVYYIAHDNPAQDDT
                     FLAQKDTFCSTQQLAGFRGLIAKVPSVFCFFGGRIATPQLQDAQTPPVFSLSQNQPNP
                     FNPATRIQFTLPEAGHVELVIYNILGQKVKTIAGGQMPVGEYSVIWDGTDYFGREVPS
                     GVYFYRLTAGEMTESKKMTLVR"
     gene            1033..1560
                     /locus_tag="AC16FU_05315"
     CDS             1033..1560
                     /locus_tag="AC16FU_05315"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR2993827.1"
                     /translation="MSCAVALLVIFNTTLYGEQIRLTNAQVGFLRAPTATQDDRLLLK
                     FDLPAGMGDRQIDIAAIRLTIPVDTSLKRPVSFTVFPVVRSWDPANVSWSRSWTAPGG
                     DFNDVYVTTVTIVPKRNRTAVIDLTPLARLWAREATGNFGVILVPIPDEGQVYKLLPV
                     TPSLAPEMGINISPR"
     gene            1618..3156
                     /locus_tag="AC16FU_05320"
     CDS             1618..3156
                     /locus_tag="AC16FU_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993828.1"
                     /translation="MIEIQNLTLALPVAKGEKIVALSNVSLSVKDGERVAVMGANGSG
                     KTVLLLAVAGLLESSAGEVTIDGLSTKARSDRAKLRDLMGIVFQDPDSQFVAPTVERE
                     IAFGLEQRGFPSQVIREKVEETLASHGLVELRKRPPVDLSGGEKQRLILGAVSVTQPK
                     YLLLDEPTSFLDFPGKELWRKWISERHEERTIIFATQYAEEALLADRLIVLDSGQIAW
                     EGKPQDFFASSGLAEKFGVPESSAKPDLSGKKVTVHHRPLLTTKEITYTYFSFGQESP
                     PALADVGLSLGEGEVIGLIGRTGSGKSTLAQILCGLLIPHKGELLQDDGSGTNTALSR
                     AVLQRIGSMAFQFPERMFFEETVYDEIAFGLRQKGELEESIAVSVYKSLEEVGLPPQA
                     FSFRSPYTLSFGEKRRVALACILALGTALVILDEPTCGLDRPGTQAVRQIIGNLAEQQ
                     KTVVVISHDITLLASAADRFIALERGHVLFDGTNGAFATQDFAQQYGVGTAPSQAASV
                     TVSQ"
     gene            3240..3311
                     /locus_tag="AC16FU_05325"
     tRNA            3240..3311
                     /locus_tag="AC16FU_05325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3272..3274,aa:Thr,seq:tgt)
     gene            3324..3405
                     /locus_tag="AC16FU_05330"
     tRNA            3324..3405
                     /locus_tag="AC16FU_05330"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3358..3360,aa:Tyr,seq:gta)
     gene            3439..3512
                     /locus_tag="AC16FU_05335"
     tRNA            3439..3512
                     /locus_tag="AC16FU_05335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3473..3475,aa:Gly,seq:tcc)
     gene            3527..3796
                     /locus_tag="AC16FU_05340"
     CDS             3527..3796
                     /locus_tag="AC16FU_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2993829.1"
                     /translation="MGYYLYILRSFVDGNLYIGQTKNLERRVALHNMGKAKSTRRRGP
                     FDLVYVEEFPTRDAAMKKGKYLKSLEGSVLKKAVVEKYQKSNSVG"
     gene            3914..4558
                     /locus_tag="AC16FU_05345"
     CDS             3914..4558
                     /locus_tag="AC16FU_05345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR2993830.1"
                     /translation="MKIGILGTGDVGQTLGRGFAQLGHDVKMGSRNPNQEKVKSWLAK
                     TGARVSAGTFAETAAFGDLAVLATAWSGTESAIRLANPKNLARKVVIDATNPLDFSSG
                     APPKLGVGHTDSGGEQVQRWLPGASVVKAFNTVGSPHMVNPEFPGGPPEMFICGDDTG
                     AKQKVTELLHAFGWSVVDIGGIQGARYLEPLAMVWILHYFNTNSGNHAFKLLRK"
BASE COUNT         1008 a         1219 c         1333 g         1084 t
ORIGIN      
        1 ttgccaattc aacggtaagc cgctgtgacg ccagtggtgt atatgcccaa aactcgaatc
       61 tggcaattct gcggaatcgt tttgatggga cggaaatgag ttctttaatc ccaggtgtgt
      121 tcatatataa gaagtcgcca ctggtacgcg ggaacacgtt tctcaatttg ggatttgccg
      181 tttgggccga aagctcggct tcggtcattg acacgaaccg gtttacgcgc acctcgcctg
      241 gggccggtct tctctcccta cgtggattaa acaaaagtaa cctgttggta cgcgggtgcg
      301 tctttactga ccctgcgcag aattcaattt ccgtgaacca atctacggtt acggtgcagg
      361 cctgttcgct ggcgggccag gccatcacgg cggtggccgc ggagaatggt tctacgctga
      421 atatccgaaa atcacggttg gctaactatt cggcttatgg tctgcatacg aactaccaat
      481 cgcaggggga tgcggggaag gtgagcaatc cgggtgagaa cttcttcggg gaagcaactc
      541 cggttgacag tctggtctac tacatcgcac atgacaaccc tgcgcaggac gataccttct
      601 tggcccagaa ggacaccttt tgttctacgc agcaactcgc cggctttcgc ggactcattg
      661 cgaaggtccc ctcagtcttt tgcttcttcg gtggccggat cgccacgcct cagttgcagg
      721 acgcgcaaac cccgccggtt ttcagtttgt cccagaacca gcccaatccg ttcaatcccg
      781 cgacccgaat tcagtttact ttacctgaag caggacacgt agagctggtg atttacaaca
      841 ttttggggca gaaggtgaaa acaatagccg gtggccagat gccagtggga gagtatagcg
      901 tcatctggga tgggactgac tattttggtc gagaagtgcc cagcggagtt tacttctatc
      961 ggcttacggc aggagaaatg accgaaagca aaaaaatgac tttggtgcga tagggcaaaa
     1021 atgatgaaaa gaatgtcttg tgcggtcgca ttgctggtaa ttttcaatac gaccctttac
     1081 ggcgagcaga ttcggttgac gaatgctcag gtcggattcc tccgggcacc cacggcgacg
     1141 caggatgacc ggcttttgct caagtttgac ctcccggcgg gaatggggga caggcaaata
     1201 gacattgctg cgatccgcct gacgattccg gttgatactt cattgaaacg tcccgtgagc
     1261 ttcacggttt ttcccgtagt acggtcgtgg gatccggcaa atgtctcctg gtcccgctca
     1321 tggacagcgc cgggcggaga tttcaatgat gtttacgtga cgacggtaac catcgtgccc
     1381 aaacgtaacc ggacagccgt catcgatctt actccattag cccgactgtg ggccagagaa
     1441 gcgacgggca attttggcgt gattttggtg ccgattcccg acgaggggca ggtctataaa
     1501 ctcctgccag ttaccccgtc tctcgctcca gagatgggaa tcaacatttc gcctcgctga
     1561 ggtatttcag aaaatctgct tgcatatcgc ccgttcaggg cgttttttgt ctgaatcatg
     1621 attgaaattc aaaatctcac tctcgccctg ccggtggcca agggcgaaaa gatagtcgcc
     1681 ctgtccaacg tcagtctttc ggtcaaagac ggtgagcgag tggcggtgat gggagccaac
     1741 ggttcgggca agacggttct gcttttggcc gtcgccggtc tgttagagtc gtcggcgggg
     1801 gaagtcacca tcgacggctt gtccacgaaa gccaggagcg accgggcgaa actgcgcgat
     1861 cttatgggca tcgtcttcca ggacccggac agccagttcg tggcgcccac cgtcgagcgg
     1921 gaaatcgcct tcggattgga acagagggga ttcccgagtc aggtcatccg cgaaaaagtc
     1981 gaagagacac tcgcgtctca cggcctggtg gagttacgca agcggccacc ggttgacctt
     2041 tccggcgggg aaaagcagcg gctcatttta ggcgcggtct ccgtcactca gccgaaatat
     2101 ctcctcctcg atgagccgac cagtttcctc gactttcccg gcaaagaact ctggcgcaag
     2161 tggatttcgg agcgtcacga agagcgaaca atcatcttcg ccacgcaata tgctgaagag
     2221 gcgcttttgg ccgatcgctt aatcgttctt gattccggcc agatcgcgtg ggaaggtaag
     2281 ccgcaggatt tttttgcttc ctccgggctg gcggaaaaat tcggcgtgcc ggagtcgtcg
     2341 gcaaagcccg atttgtctgg gaagaaggtg accgtccacc atagaccttt gcttaccacg
     2401 aaagagatta cctataccta cttctctttc ggacaggaga gtccgcccgc attggccgac
     2461 gttggtcttt ccctcggtga gggggaggtt atcggcttaa tcggtcgcac cggttcgggc
     2521 aagtccacgc tggcgcagat tctctgcggc ctgctcatcc cacacaaggg tgaattgctt
     2581 caggacgatg ggtcgggcac caacacagca ctctcaagag cggtactgca gcggataggc
     2641 agtatggcct tccaattccc ggagcggatg ttctttgagg aaacggtcta cgacgaaatc
     2701 gccttcggcc tgcggcaaaa aggcgagttg gaagaaagca tcgccgtcag cgtttataag
     2761 tcgctggaag aggtcggact tcctccgcag gcgttctcat tccgctctcc gtacaccctg
     2821 agtttcggag agaagcggcg cgtggcgctg gcctgcattc tggctctggg tacggctttg
     2881 gtcattctcg acgaaccaac ctgcgggttg gatcgccccg gcactcaagc cgttcgtcaa
     2941 attatcggaa atctggcgga gcagcagaaa acggtggtgg tcatctcgca cgatatcacc
     3001 cttctcgctt cagcagccga tcggtttatt gcgctggagc ggggccacgt actatttgat
     3061 ggaaccaacg gcgcatttgc tacccaggat tttgctcaac aatacggcgt aggaacagca
     3121 ccttctcagg cggcatcggt caccgtgtct cagtagcctc gtgaaatctc caaacgcctg
     3181 ttgacctcct gaagaccaga tgctaaaatg aacgtttgca tttgagcgtt tcgttttctg
     3241 ccggcgtagc tcagaggtag agcagctgat ttgtaatcag ctggtcaggg gttcaaatcc
     3301 cttcgccggc tgggaattta ttcggagagg taccgaagtg gccaaacgga gctgactgta
     3361 aatcagccgg caatgccttc ggaggttcga atcctcccct ctccatttat attattgtgg
     3421 cactgtccga gttttttcgc gggagtaact cagttggcta gagtcacagc cttccaagct
     3481 gttggtcgcg ggttcgagtc ccgtctcccg cttttggact aaggtcgtgg gttactattt
     3541 gtacatattg cgtagttttg tggatggaaa tctatacatt gggcagacga agaatcttga
     3601 aaggcgggtc gctttgcata atatgggcaa ggcaaagtcc acacgccgca gagggccatt
     3661 cgatttggtg tacgttgaag aatttccaac tcgggacgct gcgatgaaaa aaggaaagta
     3721 tctaaaaagt ttggaaggga gtgttctcaa gaaggcggtc gtagaaaagt atcagaagag
     3781 caactcagtt ggctagagtc atccgtacgg attggtcgcg ggttcgagtc ccgtctcccg
     3841 ctttgagtct ttcgagcgca acagcgactt ttttccgttg cattattcat tgatcaccag
     3901 agggagaaat ggtatgaaga tcggtattct gggaaccggc gacgtcggac agacactggg
     3961 aagaggattc gctcaactcg ggcacgacgt caaaatgggt tcgcgcaatc ccaaccagga
     4021 aaaggtcaaa tcttggctgg cgaaaacggg tgcgcgcgtt tcggcgggga cttttgcgga
     4081 aaccgccgct ttcggagacc tggccgtgct ggccacagcc tggtcaggca ctgagagcgc
     4141 catccgcttg gctaacccca aaaacctggc cagaaaggtg gtcattgacg ccaccaaccc
     4201 tctggatttt tcttccgggg ctccgcctaa gttaggcgtc ggccatactg attccggcgg
     4261 ggagcaggtg cagcgctggt tgccgggtgc ttccgtggtc aaagcgttca ataccgtcgg
     4321 ctccccccat atggtcaacc cggagtttcc gggcggaccg ccggagatgt tcatctgcgg
     4381 cgacgacacc ggggccaagc agaaagtaac tgaactcctg cacgcgttcg ggtggtcggt
     4441 cgtcgacatc ggcgggattc agggcgcccg gtatctcgaa cctttggcca tggtctggat
     4501 cctccactac ttcaatacga acagcggcaa ccatgccttc aagctgctgc gtaagtaacg
     4561 gactggctct ctgggccgga ttgatcacgt gttcgcctgt ccgatgtatg ctttaaggtt
     4621 acggccaggg agtcccgtct cccg
//
LOCUS       DBNGZA010000253         2634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_151138, whole genome shotgun sequence.
ACCESSION   DBNGZA010000253 DBNGZA010000000
VERSION     DBNGZA010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_151138"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="AC16FU_05350"
     CDS             complement(<1..1043)
                     /locus_tag="AC16FU_05350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR2993831.1"
                     /translation="MKVAWEVALIGLFPLSVFAQTLEYVFSPPVKVTDLPPFQTFPFI
                     YSAGQHNIAARGDTVYIVWEDIRAGDGGPRIYFAKSVDGARSFLSNVRVDDNFVPFTG
                     CHHPSMAVDQNGAIYVVWDDSRRFDLDIRFAKSTNGGQTFTPSVRVDDVNDRTVKQEF
                     ASIAVANDGLIYVVWNDYRDTLTSVYSARSTNAGLSFDNNVKVNDARAHAGFASVGAS
                     DGGRVFVAWSDLRNRQTTDLYFARSLDSGNTFQANLPVNDNLSDTTSDQAPPSLVANG
                     DKIYVAWPDKRNDDWSIYFAVSSDSGNSFFPNVKIDHAPRNTLQEFPSLAVQGNLIFA
                     LWRDQRRVPAGGEA"
     gene            1417..1740
                     /gene="csrA"
                     /locus_tag="AC16FU_05355"
     CDS             1417..1740
                     /gene="csrA"
                     /locus_tag="AC16FU_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR2993832.1"
                     /translation="MLILTRKLGESITIGDDIKVTVLGIFGRQVRLGVDAPTSVIVHR
                     EEIYKRIQEENRQAAQGKPEDLAKAKEIIDETLPPERKNNTGQTGIHYKSSEPPNPKN
                     HRRPL"
     gene            1927..2475
                     /gene="fliW"
                     /locus_tag="AC16FU_05360"
     CDS             1927..2475
                     /gene="fliW"
                     /locus_tag="AC16FU_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HIR2993833.1"
                     /translation="MKNVSFVPPAAVQGEESTPAGRNAVVVTHLGEIPFTPRQIIIVP
                     KAILGFEALKRYLLVEHSGWAPFLWLVAVDQPAVTFAVVNPLLFFPGYHIEVDRRELA
                     ELEIANPAAVHTYGIVTIPHDLRLLSVNLQGPILVNTEKNLGKQVVLSKSPFCTRHFI
                     FEELRRQFQVPSRTNLKRLSAV"
BASE COUNT          647 a          747 c          718 g          522 t
ORIGIN      
        1 gcttcccctc ctgcgggtac tcttcgctgg tcacgccata gtgcaaaaat gagattgcct
       61 tgaacggcca gcgaagggaa ttcttgtagg gtgtttcggg gggcatggtc aatctttaca
      121 ttgggaaaaa aggagttccc gctgtccgaa cttacagcga aataaatgct ccagtcatcg
      181 ttgcgtttat ctggccaggc aacgtagatt ttatccccgt ttgctacaag actgggagga
      241 gcctgatcac tagtggtatc agagaggttg tcattgaccg gaagattcgc ctgaaaagta
      301 tttccactat ccaaactcct ggcaaagtat aaatctgttg tttgccggtt acgcaaatcg
      361 ctccacgcga caaataccct tcccccgtcg cttgcaccta cagaggcaaa acctgcgtgg
      421 gctcgtgcgt cattgacctt gacgttgttg tcaaaactca agcccgcgtt tgtgctgcgg
      481 gcggaataaa ccgaagtcag cgtgtcgcgg tagtcattcc acacgacata aatcaagccg
      541 tcattcgcca ctgcaattga agcaaattct tgcttaaccg ttcggtcatt cacgtcgtca
      601 acgcgtacgg aaggagtgaa ggtctgcccg ccattggtgc ttttcgcaaa gcggatgtcc
      661 agatcaaaac ggcgggaatc gtcccagaca acgtaaatag ccccattctg gtcaaccgcc
      721 atacttgggt ggtggcagcc ggtgaagggt acgaagttgt cgtccacccg cacgttggac
      781 aagaaactcc gggctccgtc tacacttttg gcaaagtaaa tccgcgggcc accgtcaccg
      841 gcccggatat cctcccagac gatgtagacc gtgtcacctc tggcggcaat gttgtgctga
      901 ccggcggaat agatgaacgg aaatgtctga aagggaggca gatcggtaac cttgacgggc
      961 ggggagaaaa cgtattcaag tgtttgggca aaaacactaa gtggaaaaag accaataagg
     1021 gctacttccc atgcaacttt cattccagca ctcccaatga caatagtaaa acgatattga
     1081 aactaaaaaa ttataaaaat ctcaacaaaa gcggagctta aaactccgga aagaatatag
     1141 tttaagcacg cccttagggc cttagagcgg aaggcgtggc gcaggcggcg gcagagcgtc
     1201 cctctcagga gggggaaagg gctaaaaccc gcaccagacg ggcattccct gcgtattagg
     1261 agatggaaag tcagttcctc cagcactccg ccagatttac caaccgacag ccgtgccggt
     1321 gctcccgggg aaaagacggg cggcgattca agccacgctg cctttggccg ataactgaag
     1381 cacaggcagt cggtacgacc gtacgggcag aaggccatgc tgattttaac acggaagttg
     1441 ggcgagagca tcaccatcgg tgacgatatc aaggtcaccg ttctgggcat cttcggccgc
     1501 caggtgcggc tgggcgtgga tgcgccgact tcggtgatcg tccaccggga agaaatctac
     1561 aagagaatac aggaggagaa ccggcaggca gcccagggca agccggaaga cctcgccaag
     1621 gccaaagaga ttatcgacga aaccctgcct ccagaaagaa agaataacac cggccagaca
     1681 ggcatacact acaaatcatc cgagccgccc aacccgaaga accaccggag acccttgtag
     1741 gtatgtggca ctggaacaaa cccaaaaaga caaaagaaca aacttccgca gcgccggacc
     1801 gccgaccggc cgggccatct gtgaccaacc gcagtcgctt cgcaccgggt cagcgcgcac
     1861 cgcagactcc ccgccgggat ttacaacccg atctcgaccg ggggattgac gccgggttcg
     1921 tttactatga agaacgttag tttcgtaccg ccggccgccg ttcaaggcga agaatcaacc
     1981 ccagcgggac ggaacgccgt cgtcgtgacc cacctgggcg agattccttt cactccacgg
     2041 cagatcatca tcgttccgaa agcgattctg ggatttgagg cgctcaagcg gtacctcctg
     2101 gtggagcact ccggctgggc gcctttcctg tggcttgtgg ccgtcgatca gccggcggtc
     2161 acttttgccg tggtcaaccc gcttttgttc ttccccggct accacatcga agtggaccgc
     2221 cgcgaattgg ccgaactgga aatcgccaat cctgcggccg tgcacaccta cggcatcgtc
     2281 accatcccgc acgatctccg gcttttgtcg gtcaatctgc aggggccgat tctcgtcaac
     2341 accgagaaaa acttaggcaa gcaggtggtg ctctccaaaa gccccttctg cacccgccac
     2401 ttcatcttcg aggaactccg gcggcagttc caggtgccga gccggaccaa cctgaagcgg
     2461 ctgtcggccg tttaattcga accgattttc attcctgtgc cgaagcagga cgatcatccg
     2521 acgcgcgacg gggatctggt ccgcaaagga ttgacgcttt ttcgccaggg aaaactctcc
     2581 caggcggagg cgcttttgcg gcaggctttg gctgccaatg gccgcgaccc ggag
//
LOCUS       DBNGZA010000254         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2696677, whole genome shotgun sequence.
ACCESSION   DBNGZA010000254 DBNGZA010000000
VERSION     DBNGZA010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2696677"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="AC16FU_05365"
     CDS             <1..1139
                     /locus_tag="AC16FU_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2993834.1"
                     /translation="PSYVLGGQAMQVVFSPADLEAYLKQAWEVSPEHPVLIDKFLEDA
                     IEVDVDAVADGKTCFIGGIMEHIEEAGIHSGDSSCVLPPFSLSDGQIATVADYTRRIA
                     LSLDVVGLLNIQFAIKDRTVYVLEANPRASRTVPFVSKATGIPLAKVATKVALGRTLR
                     ELNLSSASPIAPPHFAVKMPVFPFIKFPGADYLLGPQMKSTGEVMGLDVDLGAALLKA
                     ELAVYQKLPAAGKVFVSVKNKDKRLIGPLVRQLSALGFQLVATQGTARALAASGLAVE
                     TIAKLSEGRPNVVDMIKSGQIQIVINTPEGFVSQRDTAAIRRAALERGIPLVTTLSGT
                     QMLVNALERLKQGKIEVRSLQEYFAPREKSEFTTVVREPIQTGE"
     gene            1272..1592
                     /locus_tag="AC16FU_05370"
     CDS             1272..1592
                     /locus_tag="AC16FU_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003782420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR2993835.1"
                     /translation="MLTVSENAAHQLKDLIKQEGEKIAGLRMMVIEGGCSGYSYQMEF
                     VEAADPRDRVVEHDGVKVFVDPASAEMLDEATIDFIDGLQGGFTIRNPKAKSSCGCGK
                     SFQT"
     gene            1651..>1995
                     /gene="nadA"
                     /locus_tag="AC16FU_05375"
     CDS             1651..>1995
                     /gene="nadA"
                     /locus_tag="AC16FU_05375"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR2993836.1"
                     /translation="MPRTFLPIPDSYKKLPQEELIARIRQHKERLGNRMVILAHHYQR
                     EEVVHLGDYLGDSYVLAKKASQANGAEHIVFCGVHFMAESADILARPGQTVYLPNPYA
                     GCPMADMAQPEDV"
BASE COUNT          438 a          549 c          608 g          400 t
ORIGIN      
        1 ggccgtcata cgttctgggc ggacaggcga tgcaggtcgt tttcagtccg gctgacttag
       61 aagcgtacct caagcaggcc tgggaggtat cgcccgagca tccggtgctc atcgacaaat
      121 ttttggaaga cgccatcgag gttgacgtcg atgccgtcgc cgacgggaaa acctgtttca
      181 tcggcgggat tatggagcat attgaggaag cgggcatcca ctccggcgat tcctcctgcg
      241 tgctcccgcc tttttccctc tcggacgggc aaattgcaac cgttgccgac tacacccggc
      301 ggattgcgct cagtctggat gtggtcggat tactgaacat tcaattcgct atcaaagacc
      361 ggacggttta cgtgctggaa gccaacccgc gcgcctcgcg caccgtgcct ttcgtgagca
      421 aagcgactgg aattccttta gcgaaagtcg caaccaaagt cgccctgggc cgcacattac
      481 gggagttgaa cttgtcgtcc gcttccccga tcgcgccgcc gcacttcgcg gtgaaaatgc
      541 cggtctttcc cttcatcaaa tttcccgggg ccgactactt attgggcccg cagatgaaat
      601 cgaccggtga agtcatgggg ctggatgtcg atctgggtgc ggcactcctc aaggccgaat
      661 tagcggttta ccagaaactg cccgccgcag gcaaagtttt cgtcagcgtc aagaacaagg
      721 acaaacggct gattggccct ttggtgcgcc agctttccgc actcggcttt cagttggtcg
      781 ccacacaggg gactgccaga gcactggcgg cttccggctt agcggtagag accattgcca
      841 aactttccga aggccggccc aacgtggttg atatgatcaa atcgggtcag attcagatcg
      901 tcatcaatac gccggagggt ttcgtttccc agcgcgatac ggccgccatc cgccgggcgg
      961 ccctggagcg ggggattccg ctggtcacca cgctttcggg aacgcagatg cttgtgaatg
     1021 ccctggaacg gctgaagcag gggaaaatcg aagtgcggag tttgcaggag tattttgcgc
     1081 cgcgggaaaa gtcggaattc acaaccgtgg tgcgggagcc gatacagaca ggggagtaga
     1141 aacttggcgg agaagttccg gagtgctccg gcttgacagg ggtattgcac ggcgtatact
     1201 acgaaagagg acaaaaacag cgtattattt agagtgaagg attccagggc gacaattttg
     1261 aaaggagcta tatgctgacc gtatctgaga acgccgctca ccagttgaag gacttaatca
     1321 agcaggaggg cgagaaaatc gccggactgc ggatgatggt gatcgaaggc ggctgctccg
     1381 gctactcgta ccagatggag tttgtggaag ccgctgaccc gcgcgaccgg gtggtggagc
     1441 acgacggggt gaaagtcttc gtcgatccgg cttcggcgga aatgctggac gaggcgacga
     1501 tcgatttcat cgacgggctc cagggcgggt tcaccatccg gaatccgaaa gccaagtctt
     1561 cctgcggctg cggcaagtcg ttccagacct aaaggaagca ggtcagcgag tcgatcgacc
     1621 cgcctgaggc gggcgcccga aaggggaagt atgccccgta cgtttttacc gatacccgac
     1681 tcctacaaga aactcccgca agaggaacta atcgcccgca tccggcagca caaggagcgg
     1741 ctggggaacc ggatggtaat tttggcccac cactaccagc gcgaggaagt ggtacactta
     1801 ggcgattact taggggattc gtacgttctg gccaaaaagg cctcccaggc taatggagcg
     1861 gagcacatcg ttttctgcgg ggtgcacttt atggccgagt cggccgacat tttggctcgt
     1921 cccggacaga ccgtctactt accaaacccc tacgccggct gccccatggc cgacatggcc
     1981 cagccggaag acgtc
//
LOCUS       DBNGZA010000255         2235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2166987, whole genome shotgun sequence.
ACCESSION   DBNGZA010000255 DBNGZA010000000
VERSION     DBNGZA010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2166987"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16FU_05380"
     CDS             <1..370
                     /locus_tag="AC16FU_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993837.1"
                     /translation="NQFLIRADAVGLGTASPSAKLDVAGSLEVGNGTPITRVLSAASA
                     LDFPSTSSQTSSYLTITVTGAADGDVASLGVPNSSGMPDADYSAWISAANTVTVRFNN
                     FSAGTLDPPSGTFRVMVTQF"
     gene            complement(435..731)
                     /locus_tag="AC16FU_05385"
     CDS             complement(435..731)
                     /locus_tag="AC16FU_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993838.1"
                     /translation="MFKKLMLTALAAGLALGPLPARAASSFDSANAQVEQAASGEGIA
                     ALDKEKPSKPKKEEKKKKKFDDFKDANGNGIDDRYERKENPKRKQKGEPGEVNF"
     gene            complement(767..>2235)
                     /locus_tag="AC16FU_05390"
     CDS             complement(767..>2235)
                     /locus_tag="AC16FU_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR2993839.1"
                     /translation="TAKGWADDETYRPNGIVPGPKGEPLEIEVWADRDNEASYQEGEN
                     ITLYFRTNRDAYVALYDIDTRGNVFLLYPQDPRDPHFVRGGVTYRLPDRGDDYDFWVT
                     GPPGAEFVGAVASLHPFDLPDDWPSYYRGSRAPGRSFSSPEKVDREDIEEFIFDLNSR
                     LVPIKRYPDDCAEDLYTFYVEPRPQTVYRPVVYDYGYYDFDFPYGSEIWIDGAYCGIA
                     PLYDYHFFVGAHIVQVYQPGCPPYVRQVYVYPRRRYSLSFSVNFNFGYRNFRYYRYYY
                     PYTYFCLISPDYRYKYRHAYYGESRYRDIYRRDVRFKSWVARDAGDRDWGKSKLLGDA
                     VRQKERRSGDRVIDRRRESKKYSPLNDAIERSRGSDRERIRTRDEEKSKPVYETYGNS
                     RKGREGSVQERKDDGRDKRDGVRYRGWEEGKKGRSSGSYEGKKERSSGNPDQPQQVER
                     GKRESGRSSEPAKVERSKQEGRGSEGRGRGSSKKGRDD"
BASE COUNT          406 a          667 c          634 g          528 t
ORIGIN      
        1 caaccagttt ctcatccgtg ccgatgccgt tggactcgga acggccagcc caagcgccaa
       61 attggacgtg gccggatcat tggaagtggg aaatggaacg ccgatcacgc gggtgctaag
      121 cgcggccagc gcgctcgatt ttccttccac cagctcacaa accagttcat accttaccat
      181 cacggtgacc ggcgccgccg atggtgatgt agcttctctt ggtgtgccta actccagcgg
      241 catgcccgac gctgattatt ccgcctggat ctcggcggcc aacacggtaa ccgtgcgctt
      301 taacaacttt tcggccggca ccttggatcc gcccagcgga acgtttcggg ttatggttac
      361 gcaattctaa gaatacagca cctaacgaaa aagccctccg cattttcggc ggagggcttt
      421 tactttcatt ggtcttagaa attcacttcc cccggttccc ctttttgctt tcttttcggg
      481 ttttcttttc tttcataccg gtcgtcgatg ccgttgccat tggcatcctt gaaatcatcg
      541 aacttctttt tctttttttc ttcctttttc ggtttggacg gtttttcctt gtcgagcgcg
      601 gcgattccct ctccgctggc ggcttgttca acttgagcgt tggccgaatc gaacgaactt
      661 gcggcccggg cggggagggg cccgagagcg agccccgctg ccagtgccgt caacatcagt
      721 tttttgaaca tcgctctcac caaattacct tcttgaccac ttgcggtcaa tcgtcgcgcc
      781 cttttttgga ggagccgcgc ccgcggcctt cgctgccgcg gccctcctgt ttggagcgct
      841 ccactttggc cggttcggag gaacggccgg actccctttt gccccgttcc acctgctggg
      901 gctggtccgg gtttccggaa gaacgctctt ttttaccttc gtaacttccc gaggagcggc
      961 cctttttccc ttcctcccag ccccgataac ggacgccgtc ccgcttatcc cggccatcat
     1021 cttttcgttc ttgaacggaa ccctcccgcc ccttgcggga gttcccatag gtttcataga
     1081 ccggcttgga tttttcttca tcacgcgtgc gaattctttc ccggtcactg ccccgtgatc
     1141 tttcaatcgc gtcgttgagc ggggaatact tcttgctctc gcgtcgccgg tcaatcaccc
     1201 gatcgccgct tcgccgctct ttctgccgca cggcgtctcc caagagcttc gatttgcccc
     1261 agtcccggtc gccggcatcg cgcgcgaccc agcttttgaa gcggacgtcc ctgcgataga
     1321 tatcccggta gcggctctcg ccgtagtagg catggcggta cttgtaacga tagtccggcg
     1381 atatcaaaca gaagtaggtg taggggtagt agtagcgata gtaccggaag ttgcggtagc
     1441 cgaagttgaa attgaccgaa aagctcaagg aatagcgccg gcgcggatag acgtaaacct
     1501 ggcgcacata cggcgggcag ccgggctggt acacctgcac gatgtgagcg ccgacgaaga
     1561 aatgatagtc gtagagtggc gcaatgccgc agtacgcgcc gtcaatccag atttctgagc
     1621 cgtaggggaa atcgaaatca tagtagccgt agtcgtagac caccgggcga tagacggttt
     1681 ggggccgggg ctcgacatag aaggtgtaca aatcctcggc gcagtcatcc ggatagcgct
     1741 tgatggggac cagccggctg ttcaaatcga agatgaactc ctcgatatcc tcccggtcca
     1801 ctttttccgg cgaggaaaag ctccggccgg gggcgcggct gccgcggtaa taggagggcc
     1861 agtcatccgg caaatcgaat ggatggaggg aggcgacggc gccgacgaac tccgctcccg
     1921 gagggccggt cacccagaag tcataatcat cgccgcgatc cggaaggcgg taggtgacac
     1981 cgccgcggac gaagtgaggg tcgcgcgggt cctgcgggta gagcaaaaat acgttgccgc
     2041 gggtgtcgat gtcgtacagc gccacatagg cgtcccgatt ggtgcggaaa taaagggtga
     2101 tgttttcccc ttcttgatag ctggcttcgt tgtcccggtc ggcccagacc tcgatttcca
     2161 ggggttcgcc tttgggcccc ggcacgatgc cgttggggcg gtaagtttcg tcatccgccc
     2221 accccttggc tgtca
//
LOCUS       DBNGZA010000256         2812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1530383, whole genome shotgun sequence.
ACCESSION   DBNGZA010000256 DBNGZA010000000
VERSION     DBNGZA010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1530383"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..>2812)
                     /locus_tag="AC16FU_05395"
     CDS             complement(375..>2812)
                     /locus_tag="AC16FU_05395"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2993840.1"
                     /translation="NTGLGDLQWNISVGGSAVARPRTYPERYYHSLPKGATDDRVGNP
                     VLLRSGGPDNFGYSWIDSDEPGGPVFNYRDISATGVQVFGLTDDNFVGPFLLGFTFNY
                     YGTDYTQFYIASNGFIGFGPTDSYSSLSNSPIPNSSPPNNILPWCWDDLFPQATSAVY
                     YQTIGSQLIIQFKNYGEFGGPGLVNAEVILNADGTILYQYLNFLNGFDKLSSTVGIEN
                     QNGTDGLEVVFDNSYLKDTLAVLFSANQWLSAAPVSGTVPPGGSVNVDVKVKTENLTP
                     GRTYRGNLLLTGNDPLRPQVPVPVRLDVVGVPNIVLSSASLDFGEVYIGYPETSQVRI
                     SNTGLDSLKVSGMDISDPQFSLLDPAILALAPGEYRDTRLQFSPTGAGNQSATLTIHS
                     NDPNDSTVTVQLRGKGVQPPAIDVVEDSLAFRLREGQSQSAMLTIENSGLGPLFWTLA
                     IGGPGSVSAAPAVSAASDATSTFKGVPDERESTPVITAAASVPNAFLYTWLDSDAPGG
                     PVFNWIDISGTGTFVSGLFDNNFVGPFPIGFSFNYYGTDYTQFYISSNGFIGFGPTSG
                     YDSPANTPLPSGSSPNNILAWCWDDLIRRSGSVFYRSAGNQLIIQFINYGQVGVTTAK
                     VNAEVILSSDGSITYQYLNFQSGFNVAGSTVGIENSDGTDGLQIAHNQSYLHDGLAIL
                     IARGLFVAPLSGTIPPGGSQDVTVRVKTNHAGIGMFRASLLVNSNDPARPQETVPVKV
                     EITPCLVHGDVNNDALTDLADITALIQDVVFASGLPNPDAGDIDCDGIRDLVDVVGLI
                     GFVVYGSPTPCCL"
BASE COUNT          632 a          769 c          822 g          589 t
ORIGIN      
        1 gcaattgcat aagcccagag ttgcaagacg gcaagaaacg gacccagcat agcccttgga
       61 cgaaaaaagg caagttgggg gactacattt gtcttcattc gtctcgggga tgacgaccca
      121 taattccccc ttttctaacg atttcttttt tttctggcgg gggtttcaga aagccgtagg
      181 gaggcaccgg taatgaagtc agtttgaact gaaagctgac cctcgtacgt taccggcgcc
      241 aaatcgcacc tgcaagttac tgcaatgcta ctaccaaaat cccggtaatc agtaatagcc
      301 ccacagggct atgaccgatt cttggaactt gccgtacctt tttcaatcag gacatttgaa
      361 actgtttcaa ctctctacaa acaacacggc gtcggactcc cgtacaccac gaacccgatc
      421 aaacccacca cgtccaccag atcgcgaatg ccatcgcagt caatatctcc agcatcggga
      481 ttcggcaggc cgctcgcaaa gaccacatcc tggatcaggg cagtgatatc cgccaggtcg
      541 gtcaatgcgt cattgttcac atccccgtgg acgaggcagg gcgtgatctc aactttcacc
      601 ggtacagtct cctgtggtcg ggcggggtcg ttgctgttga cgaggagact ggcgcggaac
      661 atcccgatac ccgcatggtt ggtcttcact cgcaccgtaa cgtcctgcga cccgcccggc
      721 gggattgttc cgctgagtgg cgcgacaaag agaccccgcg caatcaaaat ggccaggccg
      781 tcgtgcaagt agctctggtt atgagcaatc tgcaacccat cagtgccgtc gctattctca
      841 atgcccaccg tgctgccggc gacgttaaag ccgctctgga aattcaggta ctggtaggta
      901 atactgccat cggaactcag gatgacttcc gcattgacct tggcggtggt cacgcccact
      961 tgcccgtagt tgatgaactg gatgatcagt tgattgcctg cgcttcggta gaaaaccgaa
     1021 ccgctccgtc ggatcaggtc atcccagcac caggccaaga tattgttcgg agacgaacca
     1081 ctcgggaggg gggtattcgc cgggctgtcg tacccactgg tcggaccgaa gccgataaac
     1141 ccgttggagg aaatgtagaa ctgggtgtaa tcggtcccgt agtagttgaa cgaaaaacca
     1201 atggggaaag gcccgacgaa attgttatcg aacagcccgc tgacaaacgt tcccgttccc
     1261 gaaatatcga tccagttgaa cacgggtccg cccggtgcgt cactatccag ccaggtgtag
     1321 aggaaagcat tcggcacgct ggcggctgcc gtgatcaccg gtgtactctc gcgttcgtcg
     1381 ggaacacctt tgaaggtgct cgtggcatca gacgcagcgg agactgccgg ggccgcggac
     1441 actgacccgg gtccgccgat agcgagggtc cagaacaagg ggcccaggcc gctgttctca
     1501 atagtcaaca tcgccgactg actttggcct tcgcgtaacc tgaaggcaag tgaatcttct
     1561 acgacgtcaa tcgccggcgg ctggacgcct ttaccccgca actggaccgt gacggtcgaa
     1621 tcgttcgggt cattgctgtg aatggtgagc gtcgcgctct ggttgcccgc tccggtgggg
     1681 ctgaattgga gccgggtatc ccggtactct ccgggggcca gagcgagaat cgccggatcg
     1741 aggagggaaa attgcggatc gctgatgtcc atcccgctca ctttcagcga gtccagacca
     1801 gtattggaaa tgcgcacctg cgaagtttcg ggatagccga tgtagacctc cccgaagtcg
     1861 agggaggcac tggagagtac gatgttgggc accccgacga cgtccaagcg gaccgggacg
     1921 ggaacttgcg gtcggagcgg gtcgttgccg gtgaggagca ggttgccacg gtaggtgcgg
     1981 ccgggagtca ggttctcagt tttgactttt acatcaacgt tgacgctgcc gccgggtgga
     2041 acggtgccgc tcaccggcgc ggccgacagc cattgattgg ccgagaacaa aacggctaag
     2101 gtgtccttca ggtagctgtt gtcaaaaaca acttccaagc catccgtgcc attttggttt
     2161 tcgatgccca ccgtgctgct caatttgtca aagccgttca ggaaattcag gtactggtag
     2221 agaatggtgc cgtcggcgtt caggatcact tcggcattga ccagaccggg cccaccgaat
     2281 tcgccgtagt ttttaaactg aataatcaat tgactcccga tggtctggta gtaaaccgcc
     2341 gaagtggctt gggggaagag atcatcccag caccagggca aaatgttatt gggaggggaa
     2401 ctattcggta tgggcgaatt gctgagggag gaatagctgt cggtggggcc gaagccgata
     2461 aagccattgg aggcgatgta gaactgggtg taatcggtcc cgtagtagtt gaaggtgaat
     2521 ccgaggagga acggccccac gaaattgtcg tcggtcagtc cgaagacctg cacgcccgtg
     2581 gccgaaatat cccgatagtt gaagacggga ccgcccggct catcgctatc gatccaggag
     2641 tagccgaaat tgtcgggacc gccgctgcgc aggagcaccg ggttccccac ccggtcgtcg
     2701 gttgcgcctt tgggaagact gtgataataa cgctcagggt aggttctggg tctggcgacg
     2761 gctgatccgc cgaccgaaat attccattgc aaatcaccca atcccgtgtt tc
//
LOCUS       DBNGZA010000257         9069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_576671, whole genome shotgun sequence.
ACCESSION   DBNGZA010000257 DBNGZA010000000
VERSION     DBNGZA010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_576671"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="AC16FU_05400"
     CDS             complement(<1..553)
                     /locus_tag="AC16FU_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2993841.1"
                     /translation="MSASTKKILVVDDDPEVAASVADLFDPQEFSCQTAGQASSAFQK
                     LSNENFDLLLLDVNLPDRSGLDILQEIKAKHHDLPVVVITGFVSAEGALQAMMDGAYE
                     FLTKPFTAQKLSEVVKRALGQPQKKGKAAGTDWESAQEFTEDALIGKSPEIVEIAKLI
                     GQVAQSDAAVLITGESGTGKELVA"
     gene            complement(688..1926)
                     /locus_tag="AC16FU_05405"
     CDS             complement(688..1926)
                     /locus_tag="AC16FU_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR2993842.1"
                     /translation="MASEFEIVDRFLKRPAPQKNNSFARIARELGGQLHETPAGPVIH
                     LASKTSPTDRYGRLRLKEIWREDASALGGLEFLVKEPPPGNVALEDLLFLDCETTGLA
                     TAAGTYAFLVGLGAFRGQTFHLEQIFLPDYSCEKAFLQTLLKKIRPGTVLVTYNGKSF
                     DVPLLQTRFILNRLAFPLAELPHVDLLFPARRIWRYRLADLSLRSLERELLGAERERD
                     IDSGLIPGTYFHYLRSGDVSYLPDVLAHNRYDILTLAFLTLWHASLLATPQGYPNLEP
                     CDYFGLGRLYYQKHEVEKAVWCLEQAWGKAEDETLKGLAGTLLSLIHKRRENWPEATN
                     LWQAMSRLPLRWALYATEELAKHYEHRARDYSQALRMVENALATFAFSEPEHIHFPLE
                     RWEKRKVRLLCKQKRPRQLY"
     gene            complement(1944..2957)
                     /locus_tag="AC16FU_05410"
     CDS             complement(1944..2957)
                     /locus_tag="AC16FU_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2993843.1"
                     /translation="MLGQPPFQIRERPTLVGLRLHRIKQLFSPRVDGSLVPLLWVWLA
                     FASHLQADDSLAKRLDSLWIRASSGEERFRDQVEPCKQDLIAMGDTAVPYLVTKLSVE
                     DAREALTLELIFGGIGAPAVPALCAALSDSNIWVLRNIGRALAAIKDKRAVGPLIGIL
                     KNPEQGVRSQACTALGKIADTSATLALLPMLADSAETVRKSASVALGGLADLRGLLAL
                     VAALGDSYYGVRFTAMEGLVKIGKPAVEPLLKFLDSAPPKARWLAIATLGKIKDPHAE
                     GKLTAELKSTDPVSRAFAVDALVDLGTPGSRVALQKLSDPDPFVQSRLAKAKEKMIAS
                     PKK"
     gene            complement(3017..4183)
                     /locus_tag="AC16FU_05415"
     CDS             complement(3017..4183)
                     /locus_tag="AC16FU_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993844.1"
                     /translation="MRDGLLLDLDTHRGKIAIGGTGPNTYKGTYQLIIDLGGDDRYEL
                     EGGPGQNQIIIDLGGNDTYVAKSDYAIASGFMGVGILIDESGDDTYIGKNFSLGSGLF
                     GVGILWDKAGNDKYYGDQFTQGAGSFGLGALIDDGGNDQYHAAMSAQGFGFVKGAGIL
                     ADRSGNDTYFAGGKYQDVLRYKDHYLSLSQGFAYGYRPTLSGGIGLLFDGQGNDLYVT
                     DIFGQGASYWWGLGGLVDLQGNDQYISFQYAQGSAEHMTLGVLIDSSGDDLYRSKGVS
                     QGCGHDYAAGMLLDYGGNDTYIAQDLSQGAGSANGIGILIDKSGDDRYYVGDTTNTQG
                     YGNPRREYGSIGVLLDCLGKDRYDGNGKDNDVWIINSTWGVGVDGEFQQVVGGR"
     gene            complement(5001..5717)
                     /locus_tag="AC16FU_05420"
     CDS             complement(5001..5717)
                     /locus_tag="AC16FU_05420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR2993845.1"
                     /translation="MTDQQRVGNGSPQPLPAMKKSTFFLLAFLLVGWPLIVAVPEWLL
                     PVAPQDVVNNKIAELYLPTIAVEWLIFALMLLILRLEKESFGSILWRGLTVQNFAVAV
                     AFFFAAGGVLYGVESLLPTLGLKVDQNFSFLVPKTQAERFWWVALAVTAGICEEATYR
                     GYLLTRMRRFAKSWVTPVMWSSLSFGIAHSYQGEGNVLLIILYSIMLSLLFIWRKSIV
                     PGIIAHSILDLAQGLYLSGK"
     gene            complement(5761..5976)
                     /locus_tag="AC16FU_05425"
     CDS             complement(5761..5976)
                     /locus_tag="AC16FU_05425"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR2993846.1"
                     /translation="MANAERKDERRGMITGGLILVGLGFIFLLGSLNLVNWDVLWPGI
                     LIVIGLALLAGGALKCRPPSTPTDKPV"
     gene            6334..6906
                     /locus_tag="AC16FU_05430"
     CDS             6334..6906
                     /locus_tag="AC16FU_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016432.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR2993847.1"
                     /translation="MSRAGGKMQSSTSPPVEKNRRRHRLVFFSELFDSLDPELVDRLA
                     SDHEEPPPPDECTIQKVQGALNSLMPNEKMVVEEYHFQGRTLRDIALQMSRTRSEVAG
                     LLARGEEKLKTALLPYVAERFGIGAAEPERSCRVCAHPQAAAITQLLLAKPREETWSR
                     VLQQLKARYGLENPSPRTLIAHLRHPRSVP"
     gene            6912..7193
                     /locus_tag="AC16FU_05435"
     CDS             6912..7193
                     /locus_tag="AC16FU_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993848.1"
                     /translation="MKQAVLTAGQTRGQHGIKISVSYALKERIVAVATRLDRSLADTV
                     RLLLRVGLPVVERLDQDEEKMLLQAVERTRRGRKKKGFWAIMSEKPTSA"
     gene            complement(7180..7653)
                     /locus_tag="AC16FU_05440"
     CDS             complement(7180..7653)
                     /locus_tag="AC16FU_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993849.1"
                     /translation="MRADKAGSKIGEVKQANDSSENRNGYAMDDPQQKMLSARLTKPE
                     LSPREARDGLLASLCLTNRTLLKGSKPQVVADSDEHPLDRMVLALARHVFKSLGADYE
                     IPTLAQLQQAKEILDQKLKLAERLPTLKTQHDAICQLLWSKVPAPPPAAETLTLK"
     gene            7836..>9069
                     /locus_tag="AC16FU_05445"
     CDS             7836..>9069
                     /locus_tag="AC16FU_05445"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR2993850.1"
                     /translation="MRDLASEWAKHYPDFVKGLEIGKSFLGKPLSGIEVTNSKTGKAS
                     DKPALLLVGNLAGNEPVGSEVCLFAIEQAVASGDTAWENLLKRLTLYVIPCLNPDGLE
                     QFLGTSKWSSGLNLRPFDDDRDDAVDEDGPEDLNKDGIITEMLVPDPNGLWRKDSADA
                     RLLVKKKENEPGQYRRYATEGIDNDGDGLINEDGPGGTNLDRNFPAFWDMHYRQPYAG
                     MRQLSEPETRALADFVLAHPNIACVLVLGGAGGQLLRPFAGQGDEGQPVNLPEKDKQI
                     FATIGEAFKKLVPGQEFSQAEGKIAKPGLGTLLDWCYAHEGITSFLYLPWSLPADTAQ
                     KDTTGKGEPGKEMKGKKSQEGEKEPEDKKWLEFSDKRLNKGGFIEWTKFKHPTLGEVE
                     IGGFKPFVKDNPPQAFLKE"
BASE COUNT         2017 a         2490 c         2672 g         1890 t
ORIGIN      
        1 gagccaccaa ttccttgccg gtgccgcttt cgcccgtgat gaggacggcg gcgtccgact
       61 gggccacctg gccgatcaac ttggcgattt ccacgatttc cggactcttg ccgattaaag
      121 cgtcttccgt gaattcctgg gcactctccc aatcggtgcc ggccgctttc cctttcttct
      181 ggggctgccc tagggcgcgc ttgaccactt cggaaagttt ctgcgcggta aagggcttgg
      241 tcaagaactc gtacgcacca tccatcatgg cctggagggc accctcggcg gagacgaacc
      301 ccgtaatcac caccaccggc aggtcgtgat gcttggcctt gatttcctgc agaatgtcca
      361 acccggagcg gtcggggagg tttacgtcca gaagcaggag gtcaaaattt tcatttgaaa
      421 gtttttggaa ggcgctggag gcctgcccgg cggtttgaca gctaaactcc tggggatcaa
      481 agagatcggc gacggatgcg gccacctccg ggtcgtcatc cacgaccagt atctttttcg
      541 tcgacgcact catagcgaga atccggcttc tctatcatat catcggtaga aataaggcag
      601 ttcattacct gccgaaacgg ggcaaaatta ttttaaatga gactgtaata ttatggagaa
      661 ataaaaaaca aaatctggcg aattatctca gtagagctgg cggggccgtt tttgtttaca
      721 gaggaggcgg acttttcttt tttcccagcg ttcgagaggg aaatggatat gctccggttc
      781 agaaaacgca aaggttgcca gggcattttc aaccatccgg agcgcctgtg agtagtcgcg
      841 cgcgcggtgt tcgtagtgtt tggccagttc ttctgtggca tagagcgccc agcggagcgg
      901 caggcgggac atagcctgcc agaggttcgt cgcttccggc caattctccc ggcgcttgtg
      961 aatcaagctc aggagcgtcc cggccaagcc cttgagcgtt tcatcttccg cttttcccca
     1021 cgcttgttcc aagcaccaga ctgctttttc gacttcgtgt ttttgatagt acagccggcc
     1081 cagcccaaaa tagtcgcagg gttccaagtt tggataacct tgcggcgtcg ccaggaggct
     1141 cgcgtgccaa agcgttagga acgccagagt cagaatatcg tagcggttgt gcgccagaac
     1201 gtcggggagg taactcacgt cgcctgagcg caggtagtgg aagtaggtgc cgggaatcaa
     1261 gcccgaatcg atgtcccgct ctcgctcggc gcccaaaagt tcccgttcca gcgaacgcag
     1321 ggagagatcg gccagccggt aacgccaaat gcgccgggca gggaagagga gatcaacgtg
     1381 cggcaactcc gccagaggaa aggccaaacg gttgagaatg aaacgggttt gcaaaagcgg
     1441 aacatcgaaa cttttgccgt tataggtcac caggacggtg cccggcctta tttttttcag
     1501 gagtgtttgg agaaaggctt tttcgcaact gtaatcgggc aggaaaatct gttcgaggtg
     1561 gaaggtttgt ccgcggaaag cgcccagtcc gaccagaaag gcataggtgc ccgccgcagt
     1621 agccaggccg gtggtttcgc agtctaaaaa gagcaggtct tcgagggcaa cgttccccgg
     1681 tggaggctct ttcacaagaa attccaatcc gcctaaggcg gacgcatctt cgcgccagat
     1741 ctcttttagc cgcaagcgtc cgtagcggtc agttgggctt gttttcgaag caaggtggat
     1801 gaccggcccg gccggggtct cgtggagttg cccgcccaac tctcgggcga tgcgggcgaa
     1861 agaattattt ttctgtggag ccggcctctt cagaaaccgg tcaacaattt cgaattcact
     1921 tgccattgga gataagatcg caactacttc ttcggggacg caatcatttt ttctttcgct
     1981 ttggccagac gggactgcac gaaggggtcg gggtcgctta acttctggag agcgacccga
     2041 ctcccgggcg ttccgaggtc gactaaagca tccacggcaa aagcgcggga aacgggatcg
     2101 gtcgatttca attccgctgt cagtttccct tcagcgtgcg ggtctttgat ttttcccaaa
     2161 gttgcgatag ccagccagcg ggctttgggc ggggccgaat ccaagaactt gagcagcggt
     2221 tcgactgccg gtttgccaat cttgaccaag ccctccatag cggtgaagcg tacgccgtag
     2281 tacgaatcgc cgagggcggc caccagcgcc aggagcccgc gcaggtcggc aagccccccc
     2341 agcgcgaccg aagcgctctt gcgcaccgtt tccgctgaat ccgcaagcat aggcaaaagc
     2401 gcgagggtgg ccgacgtatc ggcaattttg cccagagcgg tgcaggcctg gctgcgcact
     2461 ccctgttccg gatttttcag aatgccgatg agcggcccga cggcgcgttt gtctttgatg
     2521 gcggccaagg cccggccgat gttgcgcagc acccagatat ttgagtccga gagcgccgcg
     2581 cacaaagccg gcaccgccgg cgcgccgatg ccgccgaaaa tcagttccag ggtcaaggcc
     2641 tcccgggcgt cttcaacgga aagtttggtg acgaggtagg gcaccgccgt atcgcccatt
     2701 gcgattaagt cctgtttgca cggttcgacc tgatcgcgga agcgctcctc gccggaggag
     2761 gcgcggatcc agagggagtc gaggcgtttg gcaagcgagt cgtcggcctg tagatgggag
     2821 gcaaaggcga gccaaaccca aaggagcggc actaaagagc cgtctacgcg gggggagaaa
     2881 agttgtttta ttctatgcag tcgtaggccg actaaagtcg gccgctcccg gatttgaaag
     2941 gggggctgtc caagcattgt ggattttcct ttactcccat ctcccaactc ccaaatccgt
     3001 ccttccctac cacctatcac cttccaccta ccacctgctg aaactctcca tcgacgccga
     3061 ccccccaagt ggaattgata atccagacgt cattgtcctt gccgttgccg tcgtagcggt
     3121 ctttgcccag gcagtcgagg aggacgccaa tggagccgta ctcccggcgc ggattgccgt
     3181 agccctgggt gttggtggtg tcgccgacgt agtaacggtc gtcgccagac ttgtcgatga
     3241 ggatgccgat gccgttggcc gagccggcgc cctgcgagag gtcttgggcg atgtaggtgt
     3301 cgttgccgcc gtagtccaaa agcattccgg cggcgtagtc gtggccgcag ccctgcgaca
     3361 cgcctttgga gcggtagaga tcgtctccgg aggaatcgat caagacgccc agggtcatat
     3421 gctcggctga gccctgggca tactggaacg aaatgtactg gtcgttcccc tgcaggtcga
     3481 ccaacccgcc caacccccac cagtaactgg cgccctggcc gaagatgtcg gtgacgtaga
     3541 ggtcgttgcc ctgcccgtcg aacaataacc cgataccgcc ggagagggtg gggcggtagc
     3601 cgtaggcgaa gccctgagaa agggagaggt agtgatcttt gtagcgcaag acgtcctggt
     3661 acttgccgcc ggcaaagtag gtgtcgttgc cggagcggtc ggctaaaatt cccgcgcctt
     3721 tgacaaagcc aaagccctgc gccgacatgg cggcgtggta ctggtcattt cccccatcgt
     3781 caatcaacgc cccgaggccg aaggagcccg cgccctgggt gaactggtcg ccgtagtatt
     3841 tgtcgttgcc cgccttatcc cacaggatgc cgacgccgaa aagcccggag ccgagggaga
     3901 agtttttgcc gatgtaggta tcatcacccg attcgtcgat taaaatcccc acgcccataa
     3961 agccggaggc gatagcatag tcgcttttgg ctacgtaagt gtcgttgccg cccaggtcaa
     4021 taatgatctg attctgacca ggcccgcctt ctaattcgta gcggtcgtcg ccgccgagat
     4081 caatgatgag ttgataggtg cctttgtaag tattcggtcc ggttccacca atggcaatct
     4141 tacctcgatg agtatcgagg tcgagcaaca atccatctcg cattttgcct ttatcaagat
     4201 tctcgtgaga tgaatacaac tgactgagcg cttcgcctgt tgcgaccgaa acccttcttc
     4261 ctaaactgag catgagtttg ttgtcaattt ttcgccctct ggacgggaat tctttagccc
     4321 attggtcttt tcgtttctgg aggctgtcaa tttcttcaac agaacgccgt tcgtcggaag
     4381 tatcttcgag aagtagttgg ggaaaccgga aggtagcaaa atttctatcg gagctgtcgg
     4441 ggatcaactg tatagaagaa aaaagatcga gcgctctcag cattgtccgt aagcttgttt
     4501 ttagagattc gttgagcgac ccgacgcggt cgatactttt tcgaacgtgg cgaggaattg
     4561 acgatgcgcc acctccatac tgccgtacaa gtaagtcctt taaggaacgg accggtactc
     4621 cttgttccat gctccctact atataaccgg ggggcagagc tgcgtcgaca aaaggactca
     4681 ttttataaat taaggtgttt agcagcgagt aatcgccaag agtttcctca gccaattgtc
     4741 gaagtttcgc gggcacttcc gtaggtttcc gcatcaggtt gttgacagca tacagccgga
     4801 acgaatccgc cggcacgtag tcagatcgga aggtcaaatc ctccggcgtc agtttgatgt
     4861 aggagagggc ctgcgtgaag gcggagtcca aaaggcggtc gccgaagggc gaggtcgtgt
     4921 cctgggcgaa tgcaggagag gtaaaacgag cacagcagat gaagctgatg gagcagatga
     4981 ggagacggat gcggcgcatt ttactttccc gacagataca atccttgggc caagtcaaga
     5041 atcgagtggg cgatgatgcc cgggacgatc gatttccgcc agatgaacaa aagcgagagc
     5101 ataatgctgt aaagaataat caggagcacg tttccttctc cctggtagga gtgggcgatg
     5161 ccaaaagaaa gtgatgacca catcaccggc gtcacccacg atttggcaaa gcggcgcatg
     5221 cgggtcaaaa ggtacccccg ataggtggct tcttcgcaga tgccggcagt gaccgcaagc
     5281 gccacccacc aaaaacgttc ggcctgcgtt ttgggcacca ggaaggaaaa gttttgatcg
     5341 accttcaacc ccagagtggg cagaagggac tccaccccat agagcacgcc gccggcggca
     5401 aagaaaaacg cgacggcaac tgcgaagttc tgaaccgtga gccccctcca caggatggag
     5461 ccgaagcttt ctttttccag ccgcaggatc aaaagcatca aagcgaagat caaccattcg
     5521 acggcaatcg tcggcaggta gagttccgca attttgttgt tgaccacatc ctgcggtgcc
     5581 accggcaaaa gccattccgg caccgcgacg atgagcggcc agccgaccag aagaaaagcc
     5641 agcaagaaga aggtgctctt cttcattgcc ggaagcggct gcggcgaacc gttgcccacc
     5701 cgctgttggt cggtcacagg cagaggtccc ggtacggtct caatctctgc gcgagaagac
     5761 ttagactggt ttatccgtcg gggtgctcgg cggcctgcat ttcagggcac cgcccgccaa
     5821 gagcgccagg ccgatgacga tcaaaattcc cggccagagg acgtcccagt tcaccaggtt
     5881 gagactgccc agaaggaaga tgaaccccag gccgaccaga atgagcccgc cggtgatcat
     5941 ccccctgcgt tcgtctttgc gttccgcgtt ggccatacga acctcctgaa aaagtgtcgg
     6001 ggtgtcgcgg tatcgaagtg tcgaggttgt tcataataaa tcccaaaatg atgtactcca
     6061 gcggggaata taaagtttcg gggaagtgtt gtcaggcagc ttgcataagc gcgaaagtcc
     6121 aataaatggg agttcttcag acgaaaaccc ctggtttctc cctgtccctc ccctttgagg
     6181 ggagggtggg ggaggggttt cccgacatca tggttctgag aatcatctga ggcagacacc
     6241 cttcttacat taaatcatcc tgacatcata cgcacccaaa acaattctcc ggcgggtgcc
     6301 gggtgcgctt ttttgcctgc gaaaggagaa actatgtccc gcgcaggcgg aaaaatgcaa
     6361 tcgagcacct cacctccggt agaaaaaaac cggcgccgcc accggctcgt ctttttctcc
     6421 gaattgttcg attcgctcga cccggagctc gttgaccggt tggccagcga tcacgaggag
     6481 cccccaccgc ccgatgaatg cacgattcaa aaagtacaag gggcgttaaa cagtctcatg
     6541 cccaatgaaa agatggtcgt tgaggaatat cattttcagg gtcgtacgct ccgggatatt
     6601 gcgttacaga tgtccaggac ccggtcggag gtagccggcc ttctcgcgcg gggggaggaa
     6661 aagctcaaaa cagcattact cccctatgtg gcggagcggt tcggaatcgg tgccgccgaa
     6721 ccagaaagaa gctgccgggt ttgcgcgcac ccgcaagcgg cggctatcac gcaacttctt
     6781 ttagccaaac caagggagga aacatggtca cgcgttttgc agcagttgaa agcccggtac
     6841 ggcctggaaa atccctcgcc ccgcacactg atcgcgcacc ttcgtcatcc gaggtctgtt
     6901 ccgtagggga aatgaagcag gcggttttga ccgccgggca aacccgaggc cagcacggga
     6961 tcaaaatttc cgtctcttat gcgctcaaag agcggatagt tgcagttgcc acccgattgg
     7021 accgttccct ggccgatacg gtgcggctgc tgttgcgggt aggactgccg gtggtggaac
     7081 ggctcgatca ggacgaagaa aaaatgcttt tacaggcggt ggaacgcacc cggcggggca
     7141 ggaaaaagaa aggtttctgg gcgataatga gtgagaaacc tacttcagcg tgagcgtctc
     7201 ggcggctggc gggggagccg ggacctttga ccacaacaac tgacagatcg cgtcgtgctg
     7261 ggtcttcagg gtcggcaggc gttcggccag tttgagtttt tggtccagaa tttctttcgc
     7321 ctgctggagc tgcgccagag tgggaatttc gtaatcggcg cccagcgatt tgaagacgtg
     7381 ccgggccaag gccagcacca tccggtcaag cgggtgctcg tcggagtcgg ccaccacctg
     7441 gggtttgctg cctttgagca gggtgcggtt agtcaggcag agactggcca ggaggccgtc
     7501 ccgggcctcg cggggggaga gctccggctt ggtcagccgc gccgagagca ttttctgttg
     7561 cgggtcgtcc atagcgtatc cgttcctatt ttcggacgaa tcgttcgcct gctttacctc
     7621 cccgatcttg ctccccgcct tgtcagcccg catcccggag cgtatactgg cggagcgaaa
     7681 tgatctggaa ctcccgccgc atcttttccg gttcccactt gataggagct tctatgcgcc
     7741 ggttcgcgct gaccattttc atttgcttgt tctctccacc cgctttctcg gttgcccaac
     7801 catccccgac gtgggtttat cacgcctccc ccgccatgcg cgatttggcc agcgagtggg
     7861 ccaagcatta cccggacttc gtcaaggggc tggagatcgg gaagagtttt ctcgggaaac
     7921 cgctctctgg aattgaagtc acaaacagta agaccggcaa agcaagcgac aagccggcgc
     7981 tgcttttggt cggcaatctg gcggggaatg agccggtcgg ctccgaggtc tgtttgttcg
     8041 caattgaaca agcggtcgcc tcgggtgaca cggcgtggga gaatttgctc aagcgactaa
     8101 cgctctacgt catcccctgt ctcaatccgg atgggttgga gcaattcctc ggcacttcaa
     8161 aatggagttc ggggctgaat ctccgcccct tcgacgacga ccgggacgac gccgttgatg
     8221 aggacgggcc ggaggatctg aataaggatg gcatcatcac cgaaatgctc gtgcccgacc
     8281 ccaacggcct ctggcggaaa gattccgccg atgcgcggct cttagtgaaa aagaaagaga
     8341 acgagccggg gcagtaccgg cggtatgcca ccgaagggat cgacaacgac ggcgacgggt
     8401 tgatcaacga agacggcccg ggcggcacga atctcgaccg caactttccc gccttctggg
     8461 atatgcacta ccgccagccg tacgcaggga tgcgccagct ctccgagccg gaaacgcgcg
     8521 cgctggccga ttttgtctta gcccatccga acatcgcctg cgttctggtt ctcggtggag
     8581 caggaggaca gttattgcgg cccttcgccg ggcagggaga tgagggtcag ccggtaaatc
     8641 tgcccgaaaa agataaacaa atcttcgcaa ctatcggaga agcgttcaaa aaactcgtcc
     8701 ctggccagga attcagtcag gcggagggga aaatcgccaa gccgggactc ggaacattgc
     8761 ttgactggtg ctacgcacac gaggggatca ccagtttcct ctacctgccc tggagtttgc
     8821 cggcggatac cgctcagaag gacacgaccg gcaaagggga gccggggaaa gaaatgaaag
     8881 gaaaaaagtc gcaagagggc gaaaaggagc cagaggacaa aaagtggtta gagttttccg
     8941 acaagcgact gaacaaaggt gggttcatag agtggacgaa attcaaacat ccaaccttgg
     9001 gcgaagtgga aatcggcggc ttcaagccgt tcgtcaaaga caatcccccg caggcgttct
     9061 taaaagaaa
//
LOCUS       DBNGZA010000258         4686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_48392, whole genome shotgun sequence.
ACCESSION   DBNGZA010000258 DBNGZA010000000
VERSION     DBNGZA010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_48392"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2000)
                     /locus_tag="AC16FU_05450"
     CDS             complement(<1..2000)
                     /locus_tag="AC16FU_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2993851.1"
                     /translation="MLRRWLLTVCFPVGFVAGAQAMAVTLSGNVTNPNGVGVAGVVVD
                     LYDPVTGDKIPIQKNTTDSLGNYNLNNPIGVPAATYNVAFKPLLASGVVPVIIRNYAL
                     SGNQVLNDTLPFGFVLSSFVRDTAGVGLPDIDLKVTSDSTGAAVYTPGATDPNGFYAT
                     RIPAGVFTLVYRPIAGQKLASVQYKGTVSHVDTTRNVTLRAGFFASGTVLDNLSTPVG
                     GANIKFHVSATQELVSTLNHTTDIAGHYQVTVAPGTYDISVEPLLANKIVGGKKFAVT
                     ITRDTTINFTLPHGQYLSGFARRANNLAVVPNVSIDVRDTLTSAKLVTPFDITDSTGF
                     YKIVVPAGTFNVDFLPPLATGLASVESARVAVAADRALNATLPAGVTLSGSLQRTGGG
                     GLFNVEVKFHNPSTDAKIPLANNFTDALGNYAVAAVPGTYNVEFNPPKAARRVAQEFL
                     NFALNANTTLNVTLDSGRSISGVVKDSLNNPLRDVDFNAFTLPARSKVFTPSDNTDST
                     GFYQVIVPAANLDLAYTPPLLSRFAGVMFAGLPITKDTTINLILRHGVELSGTVRDSA
                     GARVAGVEVHAFGSPETPLANGATDSAGFFAGILVPGTYSLLFVPPPASGLDSLRLTN
                     VVVQRDTTIGVTLLHPGAQTCCLITPNAIDFGQVPVGQSKDTT"
     gene            complement(2024..3517)
                     /locus_tag="AC16FU_05455"
     CDS             complement(2024..3517)
                     /locus_tag="AC16FU_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2993852.1"
                     /translation="MRAKVLWLFPLLAVFLGAGPGYSQCPLPCSQGFINVQGKVVDVN
                     SAPVHKVNLDFFPNSFGIKIATFCDKTNILGNYNTCVYPPDFYRITFNPLYPFNTHLV
                     GKETLNVDLHAPTVLRDILLEFGWVASGQATDSLGKPVDSVRLSVENLVTQRRVYLST
                     PGNRTDSLGNYRIVIPNGNYRFRYAPAAGRRQVGVQLDTVNNTAGRDTLFNVTIHSGL
                     FISGQILDSTFKPRLDSIPDVTFEIQDTLGKKIFLPRNKSDSAGKFTVVAPPGVFEFL
                     FVPPRDSHFVANRERVVRLADDTTFSQFLQRGVLLTVQVRDSLQRPVLFANLDLFRLF
                     PTPKKMFTPTDLADSAGVIKVAVPPDSYALVLKPPGGNTNFDTLFLPGLVKIFHDTTI
                     HLILPGRVFRPLPEKFTLGTNFPNPFGSQTQLPFAVQEESRVEITIYNLLGQKVRTLR
                     NETIPPGEHVVSWDGKDELGRRVPAGIYFYRMTASGFSKTRKMILIR"
     gene            complement(3520..>4686)
                     /locus_tag="AC16FU_05460"
     CDS             complement(3520..>4686)
                     /locus_tag="AC16FU_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR2993853.1"
                     /translation="IQSTRFENRYTLSQVQRDDYQATEPGLRVGAAGDWTPEVQSFLQ
                     LRLRKKFTFGTFPNRPPSAESTRVEFQAIQAYLLFRNPGGQGVALAVGKQRVRDERRW
                     LFYEYLDAARLYVYETHPIVLEASYLPSLFPLRDKKFKTWDDILFQVRYLPDAKNEAR
                     LYFLKRRDSSPRQREPVYWGLSYYGRPRRFVTGWLEASVLRGTDKQRTQQAFAVDVGG
                     TLAAVKFKVRPSVTLGYAVGSGDKVRGDAVSQEFRQTGYETNAGRFGGFAIFQYYGEV
                     LNPELANIQILTVGAGLRPHPLVSADAVFHTYRQNQLGTKIRLESQGLIAPVVVPSGV
                     DKNLGWELDFVLGIEKIWQRLNLGYAFGLFHPGQGFSPRTDQAVLNRLNLRVAF"
BASE COUNT         1039 a         1273 c         1465 g          909 t
ORIGIN      
        1 gtcgtatctt tgctctgtcc tactggcacc tggccgaagt cgatagcgtt gggagtaatc
       61 aagcaacacg tctgcgcgcc ggggtgcaag agagtcacgc caatcgtggt gtcccgttgg
      121 accaccacgt tcgtcagccg cagggagtcc agcccactgg cggggggtgg gacaaagagc
      181 agactgtagg tgccgggcac gagaatcccg gcgaagaatc ccgcgctgtc ggtcgcgccg
      241 ttggccaagg gcgtttccgg cgagccaaat gcgtgaactt ccactcccgc aacgcgagcg
      301 ccggctgaat cccggaccgt gccggaaagc tcgaccccgt ggcgcaaaat caggttgatc
      361 gtcgtgtcct tcgttatcgg aagtccggca aacatcaccc cagcgaaacg ggaaagcagg
      421 ggcggtgtat aggccagatc cagattggca gccgggacaa tcacctgata gaacccggtg
      481 gaatcggtgt tgtccgaggg ggtgaagact ttggagcgag ccggcagggt gaaggcgtta
      541 aagtcaacat cccgcagcgg attgttcaag gaatctttga ctaccccgga aatcgaacgg
      601 ccggagtcga gcgtcacatt caaggtcgta ttggcgttca aggcgaaatt caaaaattcc
      661 tgggccaccc ggcgggcagc tttaggcgga ttgaattcca cattatacgt ccccggtacg
      721 gccgccacag cgtagttccc cagagcgtca gtaaagttgt tggccagagg tatcttggcg
      781 tcggtactgg gattgtgaaa cttcacctcc acgttgaaca atccgccacc cccggtccgc
      841 tgaagggagc cggagagcgt gacacccgcc ggcagggtgg cgttcagggc ccgatcggcg
      901 gcgacggcga cccgcgcgct ttcaaccgaa gccaggccgg tcgcgagggg cggcaggaaa
      961 tcgacgttga atgtcccggc cggaaccaca atcttgtaaa atccggtgct gtcggtaatg
     1021 tcaaagggcg ttaccagctt ggcgctggtc agggtgtcac gcacatcaat actcacgttg
     1081 ggcaccacgg ccagattgtt ggcgcggcgc gcaaaaccgg aaagatattg accgtgcgga
     1141 agggtgaaat tgatggtggt gtctcgggta atcgtcacgg cgaatttctt gccgccaact
     1201 atcttgttgg ccagcagggg ctctacactg atgtcatagg tgccgggcgc tactgtcacc
     1261 tgatagtggc cggcgatgtc ggtggtgtgg ttcagtgtcg agaccagttc ctgggtcgcc
     1321 gaaacgtgaa acttaatatt ggccccaccg accggagtgg acaggttatc caggaccgtg
     1381 ccggaggcaa agaaaccggc ccggagggtt acgttgcggg tcgtgtcgac gtggctgacc
     1441 gttcctttgt actggaccga ggccagtttc tggccagcta tgggacggta aaccagagtg
     1501 aaaactccgg ccgggatgcg ggtagcgtaa aaaccattcg gatcggtcgc accgggagtg
     1561 taaactgcgg cgccggtgct gtccgaggtt acttttaagt cgatgtccgg cagccctacc
     1621 ccggccgtgt cgcgcacaaa gctcgaaagg acaaagccga aggggagcgt gtcgttcaag
     1681 acctgattgc cggaaagggc gtagttccgg ataatgaccg gcaccacgcc gctggcgagt
     1741 aacggtttga aggccacatt gtaagttgcg gccggcacac cgatgggatt gtttaagttg
     1801 taatttccca gagagtcggt ggtgttcttt tgaatgggaa tcttgtcgcc ggtgaccgga
     1861 tcgtataaat caacgaccac gccggcaact ccgaccccat tcggattggt gacgttgccg
     1921 gaaagggtaa cggccatggc ctgggctccg gcaacaaatc ccacgggaaa acagacggtc
     1981 aaaagccagc ggcgcagcat actccctccg aaaaagttgt ccgttaacga atgagaatca
     2041 ttttgcgcgt cttagaaaaa ccagaggcgg tcatccggta aaagtaaatt ccggccggca
     2101 cccgccggcc caattcatct ttcccatccc atgatacgac gtgttcccct ggtggtatag
     2161 tctcatttct cagcgtgcgt actttttggc ccaggagatt gtatatggtt atctccacgc
     2221 gcgactcctc ctgcaccgcg aatgggagtt gcgtttggga tccgaaggga ttggggaaat
     2281 tggttcccag ggtgaatttc tccggcaggg ggcggaagac ccggcccggg agaatgagat
     2341 gaatggttgt gtcgtgaaat atcttcacca ggccggggag gaataaggtg tcgaaattcg
     2401 tattcccccc gggcggcttc agcaccaagg catagctgtc cggggggacg gcgactttga
     2461 tgacgccggc tgaatcggca agatcggtgg gcgtaaacat ttttttggga gtcggaaaca
     2521 ggcggaagag gtccaaattg gcgaaaagca ccggcctctg cagggaatcg cgcacctgaa
     2581 cggtcaaaag aaccccgcgt tgcaagaatt gcgagaaggt cgtatcgtcg gcaagcctga
     2641 ccacgcgctc tctgtttgcg acgaagtggg aatcgcgcgg cggaacgaag aggaactcaa
     2701 aaactcccgg cggggccacc accgtaaact tcccggcgga gtcgctcttg ttgcggggca
     2761 gaaaaatctt tttgcccagc gtgtcctgta tttcaaaagt aacgtcggga atcgagtcta
     2821 ggcgcggctt aaacgtcgag tccaggatct gcccggaaat aaaaagaccg gagtggatgg
     2881 taacgttaaa aagcgtgtcc cggccggcgg tgttattgac ggtgtccagc tgcacgccca
     2941 cctgccggcg gccggccgcc ggagcgtagc ggaagcggta gttgccgttc gggatcacaa
     3001 tccggtaatt tcccagagaa tcggtcctgt tgcccggggt gctcaggtag acccgccgct
     3061 gggtaacgag attttcaacc gacagccgga ccgagtcgac tggtttcccc aggctgtctg
     3121 tggcctgacc ggaagcgacc cagccgaatt cgagcaggat gtcccgcaga accgtcgggg
     3181 cgtgcaggtc cacgtttaag gtctccttgc ccaccaggtg ggtattgaaa ggatagagcg
     3241 ggttaaaggt tatgcggtaa aaatcaggcg gataaacgca ggtgttgtaa ttacccagga
     3301 tgttggtttt atcacaaaac gtggcaattt taatgccgaa actgttcggg aagaaatcca
     3361 ggttgacctt gtggaccggg gcgctgttga cgtccaccac cttaccctgg acatttatga
     3421 agccctgaga acaggggagc ggacactggg aataacccgg ccctgccccc aaaaaaaccg
     3481 ccaggagggg gaagagccaa aggaccttcg cgcgcattgc taaaatgcca cccggagatt
     3541 gagccggttc aagaccgcct gatcggtgcg gggggaaaac ccctggccgg gatgaaacag
     3601 gccaaaggcg tagccgagat tgaggcgctg ccagattttc tctatcccta agacaaaatc
     3661 caattcccac cccaggttct tatctacccc ggagggaacc accaccgggg ctataagccc
     3721 ctggctctcg agacgaattt tcgttcccag ttggttctga cggtaggtgt ggaacacggc
     3781 atcggcggac accaaaggat gcgggcgcag gccggcgccg acagtcaaaa tctgaatgtt
     3841 ggccagctcc gggttcagca cctcgccgta atattgaaaa atggcgaagc cgccgaagcg
     3901 gccggcgttg gtctcgtagc cggtctggcg gaactcctgc gaaaccgcat cgccccggac
     3961 tttgtccccc gagccaacgg catatcccag ggtgacgctg ggacgcactt tgaacttgac
     4021 ggcggccagg gtgcccccca catcgaccgc aaacgcttgt tgtgtgcgct gcttgtccgt
     4081 gccgcgcaaa accgaggcct ccagccagcc ggtgacaaag cgcctggggc ggccgtagta
     4141 cgacagcccc cagtagaccg gctcccgctg gcggggtgat gagtcccggc gcttgaggaa
     4201 atagaggcgg gcctcatttt tggcgtccgg caggtagcga acctgaaaga gaatgtcgtc
     4261 ccaggttttg aactttttgt cgcgcaaagg aaagagggaa ggcaaatagg aggcctccag
     4321 aactatgggg tgggtctcgt aaacatagag ccgggcggca tccagatact cgtaaaagag
     4381 ccaccttctt tcatcgcgca cccgctgttt tccgaccgcc agagccactc cctggcctcc
     4441 ggggttgcgg aacaggaggt aggcctgaat ggcttgaaat tcaacccggg tggactcggc
     4501 gctgggagga cggttgggga aggtgccgaa ggtgaacttt ttccgcaggc gcaactggag
     4561 aaaactttgc acctccggcg tccagtcgcc cgcggcgccc acccgcaaac ccggttcggt
     4621 tgcctggtaa tcgtccctct gcacctgcga cagagtgtac cgattttcaa agcgggtgga
     4681 ctgaat
//
LOCUS       DBNGZA010000259         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2805043, whole genome shotgun sequence.
ACCESSION   DBNGZA010000259 DBNGZA010000000
VERSION     DBNGZA010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2805043"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="miaB"
                     /locus_tag="AC16FU_05465"
     CDS             <1..1139
                     /gene="miaB"
                     /locus_tag="AC16FU_05465"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR2993854.1"
                     /translation="VLGRVSQLAALKTGNPDLILGLVGCMATHLKEKLLDRAPFLDLV
                     VGPDSYRRLPQIIEEAANQPYVDVRLDKYETYADITPQRLPGVTAWVSIMRGCNKCCT
                     FCIVPFVRGRERCLPPEEVLKQVGQSVALGYKEIIFLGQTVNSYRFNGCDFGDLLSRA
                     AEIDGVRRIRFTSPHPIDFNDKIISVMASTEKICPQVHLPLQSASGPVLSRMKRRYTI
                     AYYDELLAKMRETIPDLAVTTDIIVGFPGETEEDFQKTYEYMAKVQYDGAFIFKYSPR
                     EGTKSATWPETVSEEEKVRRLETLVKLQTEISGKLNQKWVGKRVEVLVEGQAKKSKDQ
                     HYGKSPQFKTVVFDKNGENPGEFVQIFVEKATPHALVGRQAKST"
     gene            1147..>2488
                     /locus_tag="AC16FU_05470"
     CDS             1147..>2488
                     /locus_tag="AC16FU_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR2993855.1"
                     /translation="MDLILFGLVGLAALVLWVRVLALQDRYAALGLSGWGKLQIGLAL
                     FVGGALYIILQRALGLTELLTLFGLPIGLLGVASAWAVGLFLIFSSPVRWLSALRARQ
                     EQTATREDELSLLESIREAAWQPYTLVEILQYALKEMLRFSGVEAGIIFVANLTRKEL
                     ILAAASGLSREQMKRFEKIALGGDLFSRSFTNRKPVLIGNLSLHERATRGLLQKNEFR
                     SLACVPLLVKDGVVGAGLFLSPREFALDQKQVASLAQAGNLLGSAIETARLQREVARR
                     TERVGQLEEGYKSITDLISFVWQAAGWEQAFDRLFVALLPRLGGEVACVVQKESGDAY
                     RVIASSDPTCFGKELGSPFTPHFAEAVRTGRPLVVPGLLDTPLGDRIASAVIAPLFFW
                     GESQGFLVIGSARPDPDYSQAELAQMDFAARLAAVAATQITAQRAVVTTEDKIAA"
BASE COUNT          506 a          715 c          729 g          538 t
ORIGIN      
        1 gggttttagg ccgggtgtcg cagttggcgg cgctcaaaac cggaaaccct gatctgattt
       61 tggggctggt cggctgtatg gcgacgcatc tgaaagaaaa acttttggac agagcaccgt
      121 ttctcgattt agtcgtcgga cccgattcct accgccgcct gccgcaaatc atcgaagaag
      181 cagccaatca accctacgtc gacgtccggc tcgacaagta cgagacctac gccgacatca
      241 ccccgcaaag gcttcccggc gtcaccgcct gggtgtccat tatgcgcggg tgcaacaagt
      301 gctgcacctt ttgcatcgtc ccctttgtgc gggggcggga gcgttgcctg ccgccggaag
      361 aagtgctcaa gcaggtgggg cagtcggtgg cgctgggata taaggaaatc atcttcttgg
      421 gccagacggt caattcctac aggtttaacg gctgcgattt cggcgatctg ttatcgagag
      481 cagcggaaat tgacggcgtc cggcgcatcc gcttcacctc gccgcacccg attgatttca
      541 acgataagat catctccgtc atggcctcga cagaaaaaat ctgcccgcag gtgcacctgc
      601 ccttgcagtc ggcctccggt ccggtgctct cccggatgaa gcggcgctac acgatcgctt
      661 actacgatga actcttagcg aaaatgcggg agacgattcc tgatttagcg gtgacgaccg
      721 acatcatcgt tggtttcccc ggggagacgg aggaagattt ccagaaaacc tatgaataca
      781 tggcaaaagt ccagtacgac ggcgccttca ttttcaaata ttcgcctcgc gaaggcacca
      841 agtcggcgac ttggccggag acggtttccg aagaagaaaa agttcgccgg ttagaaacgc
      901 tggttaaact ccagaccgaa atttcaggca aacttaacca gaaatgggtg gggaagaggg
      961 tggaagttct ggttgaaggc caagcgaaga aatcaaaaga ccagcactac ggcaaatcgc
     1021 cgcagttcaa aaccgtcgtc ttcgacaaaa acggggaaaa cccgggagag ttcgtgcaaa
     1081 tatttgtaga aaaagccacc ccgcatgcac tggtggggag gcaggcgaag tcaacctgat
     1141 taagtcatgg atctaattct cttcggactg gtcggtcttg ccgcgctggt actctgggtg
     1201 cgggttctgg cgctccagga ccgctacgct gctctggggc tttccggctg gggcaaactt
     1261 cagatcggcc tggccctctt cgtcggcgga gcactctaca tcatcctcca gcgcgccctg
     1321 gggctcactg aacttctgac actctttgga ttgcccattg gtttgctggg cgtcgcttcc
     1381 gcctgggcgg tggggttgtt cttgattttc tcttctcccg tgcgctggct ttccgccctc
     1441 cgtgcacggc aggagcaaac cgccacccgc gaagatgaat tatctctctt agaatcgatc
     1501 cgcgaagctg cctggcagcc ctacacgtta gtcgaaatcc tccagtacgc gctcaaggag
     1561 atgctccgct tctccggggt ggaagcgggt atcatcttcg tcgccaatct cacccgcaag
     1621 gaattaatct tagccgcggc ctccggcctc tcccgcgaac agatgaaacg gttcgagaaa
     1681 atcgctttgg gcggtgatct cttctcccgt tcctttacca accgaaagcc ggtgttgatt
     1741 ggaaatctgt cgttgcacga gcgggccacg cgcgggctgc tccagaaaaa cgaattccgc
     1801 tcgctggcct gcgtgccgct tctggtgaaa gacggcgtgg tcggcgccgg tttattcttg
     1861 agtccgcgcg aattcgcttt ggatcaaaaa caggtggcca gtttagccca ggccggtaat
     1921 cttttgggtt ccgccatcga aacggcgcgc ctgcagcgcg aagtggcccg gcgcacagag
     1981 cgggtcggac aactggaaga agggtacaag agcatcaccg acttaatttc cttcgtctgg
     2041 caggccgccg gctgggagca ggcgttcgac cggctcttcg ttgcgctttt accgcgcctg
     2101 gggggcgaag tggcctgcgt ggtgcaaaaa gaatcgggag atgcgtacag agttatcgct
     2161 tccagtgatc cgacctgttt tgggaaagag ctcggttctc ccttcacccc gcacttcgcg
     2221 gaggcggtac gaacgggccg tccgctggtc gttcccggcc tgctcgatac accgctgggt
     2281 gaccgcattg cgtcagccgt gattgcgccg cttttcttct ggggtgaaag ccaggggttt
     2341 ttggtcatcg gttccgcccg gcccgatcca gactattcgc aggcggaact ggcccagatg
     2401 gatttcgccg cgcggctggc cgccgtggcg gccacgcaga tcaccgccca gcgggccgtc
     2461 gttaccaccg aagacaagat tgccgctt
//
LOCUS       DBNGZA010000260         4881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_577154, whole genome shotgun sequence.
ACCESSION   DBNGZA010000260 DBNGZA010000000
VERSION     DBNGZA010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_577154"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="AC16FU_05475"
     CDS             <1..1088
                     /locus_tag="AC16FU_05475"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR2993856.1"
                     /translation="AVREVVKRAGIEPNEVDEVMLGHVVQAGAGQAPARQAAIHGGIP
                     SSVPCMTVNKVCGSGLKTVMCAAQSIRAGDNQVVLAGGMESMSNTPHLLHGAKLGFRF
                     GDQELKDAMILDGLWDSFNNFHMGSAAELTAKKSNISREEQDEFAYNSHMKAAAAIRN
                     GKFKAEIVTVEVPQKKGDPAKFELDECVRPDTSIEKLSKLRPAFEKNGTVTAGNAPGL
                     NDGASTVMVASAEFAKKKGLKPLAKIVGYAAAGTDPELLFYAPIYAVRKLMKQIGVDI
                     NHWDLIEVNEAFAAQVLADGKELGWDWKKVNVNGGAVALGHPIGASGARILTTLLYAL
                     QDRGLKKGLATLCLGGGNALALAVEMI"
     gene            1125..1511
                     /locus_tag="AC16FU_05480"
     CDS             1125..1511
                     /locus_tag="AC16FU_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2993857.1"
                     /translation="MPETKRIGSVEDLEVYRKLCSLHLDVHNFSLKFPKFEMYELGSQ
                     LRRSSNAVPANLAEGWNNKHTNIYLEGINRAFGELRETLHHLRMASQKGYLQDTVHRN
                     LASRYEECGRMLRGLERALVRQRNGT"
     gene            1602..2741
                     /locus_tag="AC16FU_05485"
     CDS             1602..2741
                     /locus_tag="AC16FU_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2993858.1"
                     /translation="MDFELTENQILLRDTAREFAQKRVAPLAQEMDEKEAIPPDLLKE
                     TADLGYFGLLIAEQYGGLGVDMLSYVLAMEEIARASAGLMITISVHNSLVAQGLVHFG
                     SEELKQKYLPKLASGELVGAYALTEPGSGTDAAALITSAVRQNGHYLVNGTKSWVTNA
                     GFAGLYSVFVSTNPALKSKGISCLIVERDLLGLTLGAKEKKMGIKCSDTREISFQDAR
                     VPAANLLGVEHDGYKIALALLDFGRLGVGAQSIGIAQAALDEAVKYSKERQQFGQPIA
                     NFQGIQFKLADMATQIEAARLLVYQAAALEDKPGRWSKEIAMAKLFASEVANYVANEA
                     VQIHGGYGYIKEYAVERYFRDARVTEIYEGTSEVQRMVISRAILT"
     gene            2745..3878
                     /locus_tag="AC16FU_05490"
     CDS             2745..3878
                     /locus_tag="AC16FU_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR2993859.1"
                     /translation="MHLEKIYQELRDRIRTFADRELAPHAKDVDEKGEVRRENVAALA
                     REGLLGMLIPKEYGGFAEGTLAYVIALEEVSRACGSTGIIMAAHNSLCCFPIYRFGTE
                     AQRHRYLPDLAAGRVLGAFGLTEPQAGSDAGNTKTTAVLKDGHFIINGQKCWITNASF
                     AGTFVITARTSPGTGADGISAFIVLRTTPGFTSGKKENKMGLRGSDTAFLHFTDMPVP
                     QENLLGPLGVGFKQFMQTLDGGRIGIAAMALGIAQGALDCALAYAGERHQFGQPIGSN
                     QAIQWKLADMTTQLAAARLLTYRAARLKDKGEKTTRESSMAKLFASEAGRYVCYQAIQ
                     ILGAAGYTNRYPVERMYRDVKLCEIGEGTSEIQRLVIAREVLK"
     gene            4010..>4881
                     /locus_tag="AC16FU_05495"
     CDS             4010..>4881
                     /locus_tag="AC16FU_05495"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR2993860.1"
                     /translation="MLFEEVKKIWMDGKLVDRDQASVSILAHVIHYGSSIFEGIRAYN
                     TPDGPAVFRLPEHLERLYNSCRIYRMEIPYTRKQLLDATLEIIRLHDVRHWTSPYIRP
                     VVYRGLRHLGVDPTGCPVNVAIAVLDMGHYLGEDAKRNGVRVCVSSWNRIAPNTLPAM
                     AKASANYMNSQLIKMEANAILKPDASKKEKVEGISLDVSGHVSEGTGENIFLVRDGAL
                     VTPSFASSILPGVTRNTVIALAQEMGIKVIEEQVPREALYIANEVFFTGTAVEIVAVR
                     SIDGIEVGDPALSP"
BASE COUNT         1072 a         1405 c         1459 g          945 t
ORIGIN      
        1 tcgcggtgcg ggaagtggtc aagcgcgcgg gtatagagcc gaatgaagtc gatgaggtca
       61 tgctgggcca cgtagtgcag gcgggcgccg ggcaggcccc ggcccggcag gcggcgatcc
      121 acggcgggat tccatcctcg gttccctgta tgacggtgaa caaggtctgc ggctcagggc
      181 taaagacggt gatgtgcgcc gcgcagtcga ttcgagcggg ggataaccag gtcgtcctcg
      241 cgggcgggat ggagtcgatg tccaacacgc cgcacctcct gcacggcgcc aagctggggt
      301 tccgcttcgg cgaccaggaa ttgaaagacg cgatgatttt agacgggttg tgggactcct
      361 tcaacaattt ccacatgggc agcgcggcgg aactgaccgc caaaaagtcg aacatttccc
      421 gggaggagca ggacgaattc gcctacaaca gccatatgaa ggccgccgcg gcgatccgca
      481 acggcaagtt caaagccgaa atcgtcaccg tggaagtgcc gcagaagaaa ggcgacccgg
      541 cgaagttcga gctggacgaa tgcgtccgcc ccgacacgtc gatcgagaaa ttgtcgaaat
      601 tgcgtccggc gttcgagaag aacggcaccg taaccgccgg caatgcgccg ggcttgaacg
      661 acggggcttc cacggtcatg gttgcctccg cagagttcgc caagaaaaaa gggctgaagc
      721 ccctggccaa gatcgtgggc tacgccgccg ccggaaccga tccggagctg ctcttctacg
      781 ccccgattta cgccgtgcgc aagttgatga agcaaatagg ggtggatatc aaccactggg
      841 acttaatcga agtgaacgag gccttcgccg cgcaggtctt agctgatggg aaagagctgg
      901 gctgggactg gaagaaggta aatgtcaacg gcggcgcagt ggctttgggc caccccatcg
      961 gcgcctccgg cgcccgcatt ctcaccacgc tcctctacgc tctgcaggac cgggggctca
     1021 agaaagggct ggcgacgctc tgtctgggcg gcgggaacgc cttggcgctg gcagtggaaa
     1081 tgatatgaga cggtggtggg tgatgggtgg taggtggtag gggaatgccg gaaacaaagc
     1141 ggatagggag cgtcgaggat cttgaggtct accgaaaact ctgttccctg cacttagacg
     1201 tacacaactt ttccttaaag tttccgaaat ttgaaatgta tgagttgggg agtcagctcc
     1261 ggaggtcatc gaatgcagtt ccagcaaact tagctgaggg atggaacaat aagcacacga
     1321 atatctattt agaagggatc aaccgggctt tcggcgaact gcgtgaaacg ctccatcatc
     1381 tccgtatggc ttctcaaaaa gggtatctcc aggatacggt ccaccgaaat ctggcctcaa
     1441 gatatgaaga atgcggccgc atgctccgcg gccttgagcg tgccctcgta cggcagcgca
     1501 atggtacata gccttgtttt gcctatcacc caccacctat cacctaccac cttgtacctg
     1561 caaccgagca attcgtttcc atttgtgcca caggagtgac gttggatttt gagctgaccg
     1621 aaaaccagat cctcttgcgc gacacggcgc gggagttcgc ccagaaacgg gtggctcccc
     1681 tcgcgcagga aatggacgag aaggaagcca tcccgcctga tctactcaaa gaaaccgctg
     1741 atttaggcta ctttggtctt ttgatagctg agcagtatgg cggtttgggc gtcgatatgc
     1801 tgagttacgt cctggctatg gaagagatcg cccgggcgtc cgccgggctc atgatcacca
     1861 tctcggtgca caactcgctc gtggcgcagg ggctcgtcca ctttggttcc gaggagttga
     1921 agcagaagta cctgccgaag ctcgcctcag gcgaacttgt cggcgcttac gccctcacgg
     1981 agcccggctc gggcaccgac gcggcagctc tcatcacctc agccgtgcgg cagaacggcc
     2041 actaccttgt caacggcacc aagagctggg ttaccaatgc cggtttcgcc ggactttact
     2101 ccgttttcgt ctccaccaat cccgccttga aatccaaagg catttcctgt ttgatcgtcg
     2161 agcgcgacct attggggctt acgctgggtg ccaaagagaa aaagatggga atcaagtgct
     2221 ccgacacccg cgaaatttcg ttccaagatg cccgggttcc ggcggccaat ctcttgggcg
     2281 tggagcatga cggctacaag attgccctgg cgcttctgga cttcgggcgt ctgggcgttg
     2341 gagcacaatc gattggtatc gcgcaggccg ccttggatga agcggtcaaa tactccaaag
     2401 aacggcagca gttcggccag ccgatcgcga actttcaggg tattcagttc aaattagccg
     2461 acatggcgac ccagatcgaa gcagcccggc ttttagttta ccaggcggcg gcgctcgagg
     2521 ataaaccggg acggtggtca aaagaaatcg ccatggccaa actttttgcg tcggaagttg
     2581 ccaactacgt ggcgaatgaa gcggtgcaga ttcacggcgg ctacggttac atcaaggaat
     2641 acgccgtcga acgctacttc cgggacgcgc gggtgaccga aatctacgaa ggcacctccg
     2701 aagtccagcg catggtcatc tcccgcgcca tattgaccta accgatgcac ttggaaaaaa
     2761 tataccagga gttgcgcgac cgaatccgta cctttgccga cagggaactc gcaccccacg
     2821 ccaaagatgt ggatgaaaaa ggcgaggtgc ggcgagaaaa cgtggcggcc ctggcgcggg
     2881 aaggtctcct gggaatgctc atcccgaaag aatacggcgg ctttgcggag ggcaccctcg
     2941 cctacgtcat tgccctggag gaggtctcgc gcgcctgcgg ctccaccggc atcatcatgg
     3001 cggcgcacaa ttcgctctgc tgttttccca tctaccgttt tggaactgag gcgcagcgcc
     3061 acaggtatct cccggacctc gccgccgggc gcgttttagg tgctttcggg ctcaccgagc
     3121 cgcaagcggg ctcggacgcc ggcaacacca aaaccaccgc cgtgctcaaa gacggtcact
     3181 tcatcatcaa cggccagaag tgctggatca ccaacgccag tttcgccggt accttcgtca
     3241 ttaccgcccg caccagtccg ggcaccgggg ccgacggcat ctcggccttc atcgtcctgc
     3301 gcaccacacc cggttttact tcaggtaaaa aggagaacaa gatgggcctg cgcgggtcgg
     3361 acacggcatt tttgcacttc accgatatgc cggtgccgca agagaatctg ctcggtcccc
     3421 tgggcgttgg gtttaagcag ttcatgcaga ctctggacgg cggccgcatc ggcattgcgg
     3481 ccatggcgtt gggaatcgct cagggggcct tagactgtgc cctggcctat gccggagagc
     3541 ggcaccagtt cggccagccc atcggttcca accaggccat ccagtggaag ttagccgata
     3601 tgacgacgca gttagccgcc gcccggcttt tgacctaccg cgccgcgcgg ctcaaagata
     3661 aaggggagaa gaccacccgc gaatcatcga tggcgaaact gtttgcctcc gaggccgggc
     3721 gttacgtatg ttatcaggcg atacagattc tgggcgccgc cggctacacc aaccgatatc
     3781 cggtggaacg gatgtaccgc gacgttaaac tgtgcgagat cggcgaaggt acctcggaga
     3841 ttcaacgact ggtcattgcc agagaggtgt tgaaatagtg tcgaggtgtc gcggttcgac
     3901 aggctcaccg tccaaagcaa agtcgaggga aagtgtcgag gtgtcgaggt tgaaagagtg
     3961 ataagcgccc atcttgagaa taattacggc ctctgtcagg gaggagacca tgctgtttga
     4021 agaggtaaag aaaatctgga tggatggaaa attggttgat cgcgaccaag cctcggtttc
     4081 gatcctcgct cacgtcatcc actacggctc ctcgattttt gaaggcatcc gcgcctacaa
     4141 cactcccgac ggcccggccg ttttccgcct gccggagcat ttggagcggc tctacaattc
     4201 ctgccgcatc taccggatgg aaattccgta tacccgcaag caactgttgg acgcgaccct
     4261 ggaaataatc cgcctgcacg atgtccgcca ctggacgtcc ccttacatcc ggccggtggt
     4321 ctaccgtggc ctgcggcatt taggcgtcga ccccaccggc tgccccgtca atgtcgccat
     4381 cgcggtcctg gacatgggcc actacctggg tgaggacgcc aagcggaacg gcgtacgggt
     4441 ttgcgtctct tcctggaacc ggattgcgcc gaacacactg ccggccatgg ccaaggcctc
     4501 ggccaattac atgaacagcc agttgattaa aatggaagcc aatgccattc tgaagccgga
     4561 tgcgtccaaa aaagaaaaag tcgaaggcat ctcgctggac gtctccggac acgtctccga
     4621 aggcacgggc gaaaacatct tcttagtgcg cgacggagct ttagtcacgc cgtccttcgc
     4681 gtcatcgatt ctgccgggag tgacccggaa caccgtcatt gccttagcgc aagagatggg
     4741 gattaaggtg atcgaagagc aggtaccccg ggaagccctc tacatagcca atgaagtctt
     4801 cttcaccggc accgccgtgg agatcgtcgc ggtccgctcc atcgacggca tcgaggtcgg
     4861 cgaccccgcg ctctccccgg a
//
LOCUS       DBNGZA010000261         6981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2805447, whole genome shotgun sequence.
ACCESSION   DBNGZA010000261 DBNGZA010000000
VERSION     DBNGZA010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2805447"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16FU_05500"
     CDS             complement(<1..391)
                     /locus_tag="AC16FU_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993861.1"
                     /translation="MNRHVIPTAFVLLLASISSASVAGEHTSPQKIAPALTTRWQTSP
                     DSSVKVWVFFTDKGSADPKVQAQALTNLNQYFSPRALARRQRVNRGQLVFDAADLPVS
                     SNYLSQLSHSGITADQVSRWLNAASVSL"
     gene            complement(570..2846)
                     /locus_tag="AC16FU_05505"
     CDS             complement(570..2846)
                     /locus_tag="AC16FU_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993862.1"
                     /translation="MRNRWEVSKLKFFGVSVVIALLSFFATYARVNRIQSEGRESGAA
                     TTFTVNLTGDESDSAPGSGGCDIDPITLGFQCTLRAALEEANAHPGVDSINFNVAAVG
                     VPTISPGSALPTITEAVVIDGTTQPTGNRIELNGSGAGFANGLVISAGGSTVKGLVIN
                     RFGNNGIGLVTSGGNVLVSNFIGTDVTGGIALGNGQSGVHINNSPDNIIGGTAAAERN
                     IISGNGMWGVRIQGSSATKNLVQGNFIGTDVRGVARLGNGLDGVIIGFASDNTVGGTT
                     AGTVNIISGNGQSGVFIAGNRATGNVVQGNFIGTDVTGTGALGNSSSGVAFDNAPNNT
                     VGGRTPGARNVISGNNQSGVLIAGSKATGNLVQGNFIGTDVTGAGALGNSGSGVTIDN
                     VSNDTVGGTAAGARNVISGNNQSGVEIGGIVATGNVVLGNCIGTDVTGTAALGNSYAG
                     IFIRETAENNTLGGTVAGARNVISGNERYGVVMAGGATGTIVAGNFIGTDITGTVALG
                     NAENGVSIFGFSSNNTIGGGTEAAGNTIAFNGNEGMVVDLTSRGNSCLHNSIFSNGAR
                     GIDLFPGGVTLNDLGDVDAGSNDLQNFPVFTSAGSVGATVTVQGSLNSRANGTFTLEF
                     FASDSCDPSGYGEGQNFFGSVLVTTDGSGNASFNVTFPVAVAAGKFITATATDSEGNT
                     SEFSQCLTVCALSGDVTNDAVIDIVDLAALVQDIVFGLPLPNPAAGDANCDGVRDIVD
                     VVGLIQYVVFGSPTPCCL"
     gene            complement(3075..5249)
                     /locus_tag="AC16FU_05510"
     CDS             complement(3075..5249)
                     /locus_tag="AC16FU_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993863.1"
                     /translation="MQKAYRFFTVVIFLLASRTPTVYGFDWPELLLYQKNGAAAADQF
                     GYTVAGAGDVNGDGRSDFIVGAPFADPGGLANAGSAFVYSGATGLLLYQKNGPTAGDQ
                     FGQSVAGAGDVDGDGKADFIVGAPFADPGGVGFAGSAFVYSGATGALLFQKNGAAAFD
                     LFGFSVGGVGDATGDNRSEFIVGAHFADPGGLLDAGSAYVYSGATGTLLYQKDGAEGD
                     QFGVSVAGAGDANGDNRADFIIGARLADPTLGLIDAGSAFVYSGATGALLYRKDGAVT
                     GDELGWPVSGAGDVDGDGRGDFLISARLADPGAVVNAGSVFIYSGATGTLLYQKDGAA
                     VEDRFGFWAAGAGDVNGDGKAEFIVGAFKADPGGLADAGSVFIYSGATGGLLFQRNGA
                     AGDSLGFSVAGAGDVNGDGRAEVIVGAPFADPGALSAAGSAFVYALRSVRIASLADVP
                     NDQGKQVIFRWKSFPGNDSLMDLFIVYRRLDSTFNSPGKGVLAADALPLPPGDWLQVA
                     VLAATGDTAYATIVPTLVDSTLAGTKYSVFFVRGRSTNPLLSIDSPLDSGYSIDNLVP
                     APPANLSASGTPGGIVLAWRPVPDFDFDFFFLYRDTVAGFTLSPANRIKATSDTGAVD
                     SPADASKTYYYRVTAVDFSGNEGPPSNEGTAVCAGVRGDVTDDAVADIVDIVALIQEI
                     VFGTPLTNPHAGDTNCDDVRDIVDIVLLIQFVVFGSPTPCCL"
     gene            complement(5385..>6981)
                     /locus_tag="AC16FU_05515"
     CDS             complement(5385..>6981)
                     /locus_tag="AC16FU_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2993864.1"
                     /translation="NNKDGVEISGSSTTGNQVLGNFIGTDATGSFCLGNDSNGVHIDD
                     APNNVIGGTAAGAGNVISANGRGILVENAAATGNLVQGNFIGTRATGTAPLGNARSGV
                     FIRNSASNNTVGGTVAGARNVIAANSFGITLGASGNQILGNFIGTDVSGTAGVGNIAD
                     GVFIDGSSNSIIGGAAAGARNVISGNGDDGVKMVNGVTGTVVQGNFIGSDVSGTVPLG
                     NTFSGVSFFNSSGNTVGGTTAGARNLISGNHTNGVFFQNATGNFVQGNLIGTDISGTA
                     ALGNGDAGVWLELAPSNNTIGGEIGGAANTIAFNGSQGVGMGFQPGTGNSSHRNTIFF
                     NGLLGIDLGADGVTQNDTGDADAGANNLQNFPVLTSALSVGGSTTIEGTLNSTANDSF
                     RLEFFSNDSCDASGYGEGQTFVGSTSVLTDGSGNASINVTLPVAVPGGKFITATATHS
                     AGNTSEFCQCLTVCAGLRGDVTTDGVIDILDLAALVQDIVFGVPLADPAAGDANCDTV
                     RDIVDLALLVQYIVFGSPNPCCL"
BASE COUNT         1483 a         2140 c         1983 g         1375 t
ORIGIN      
        1 tgagcgaaac gctcgccgcg ttgagccagc gcgagacctg atccgcggtt attccggaat
       61 gggaaagctg cgagaggtaa ttagaggata ccggcagatc ggcggcatcg aataccaatt
      121 gaccgcgatt tactctttgt ctgcgcgcca aagcgcgggg agaaaaatac tggtttaagt
      181 tcgtgagggc ttgcgcctga acttttgggt cggcgctgcc cttgtcggtg aaaaagaccc
      241 atactttgac ggacgaatca ggcgaagtct gccagcgggt agttagtgcg ggggcaattt
      301 tctgaggact ggtgtgttct ccggcgactg aggccgagga aattgatgcc agcaagagga
      361 caaaagcagt cggaatgacg tgccggttca ctaactatta ttacggaaga tttccgtcta
      421 atgcgagggg ataatacttt aagaagctat tctaatctcc gatccccgtt ttgataatga
      481 ctctccctcc cctttgaggg gagggttggg gaggggtcag acaagatgtc tgacctatgg
      541 aatgaaattg gtcttctaat ccctgctcat cacaaacagc aaggcgtagg gctgccgaac
      601 accacgtact gaatcaagcc caccacgtcc acaatgtctc gaaccccatc gcagttcgca
      661 tcacccgcgg cgggattagg tagaggcagg ccgaagacaa tgtcctgcac aagcgcggct
      721 aagtccacga tgtcgatcac cgcgtcgttg gtcacatccc cgctgagagc gcaaacggta
      781 aggcactgcg agaactcaga agtgttgcct tcagagtcgg tagcggtggc ggtaatgaat
      841 ttaccggcgg cgactgcgac cgggaaggtg acgttgaagc tggcgttacc acttccatca
      901 gtggtcacca gcacggagcc aaagaaattt tgcccctctc cgtagcccga agggtcacag
      961 gagtcgctgg cgaagaattc gagggtaaaa gtgccattcg ctcggctgtt gaggcttcct
     1021 tgaacggtga ccgttgcacc gacactgccg gccgaagtga aaactggaaa attttgcagg
     1081 tcattggagc cggcgtcaac atcccccaga tcattcaagg ttaccccacc ggggaaaagg
     1141 tcaatacccc gagcgccgtt tgaaaaaatg gaattgtgca ggcaactgtt gccgcggctg
     1201 gttaaatcta ccaccatgcc ctcgttaccg ttgaaggcga tggtgttgcc cgcggcctcc
     1261 gttccacccc cgatggtgtt gttgctggaa aagccaaaaa tcgacacgcc attttcggcg
     1321 ttgcccagag caacggttcc cgtgatgtcg gtcccgatga agttacccgc cacgatggtc
     1381 cccgttgcac ctcctgccat cacgacgcca taacgctcat tgcccgagat gacattgcgc
     1441 gctcccgcca ccgtgccccc cagggtgttg ttttcggcgg tctcccgtat aaaaatgcca
     1501 gcataggagt tgcccagagc ggcagtgccg gtaacgtcgg tgccaatgca gttccccagg
     1561 accacgttcc cggttgccac aatcccgcct atttcaacgc ccgactggtt attaccggaa
     1621 atgacgttgc gcgctccggc agccgttccc ccgacagtgt cgttggaaac attatcaatg
     1681 gtcaccccag agcccgagtt gcccagagct cccgcgccag tgacgtcggt gccaataaag
     1741 ttcccttgca ccagattccc ggtagccttg ctcccggcta tgagaacgcc tgactgatta
     1801 ttgcccgaaa tgacgttgcg cgccccggga gtccttcccc cgacggtatt gttcggagca
     1861 ttatcaaaag ccacgccaga gctcgaattg ccgagagctc ccgtgccagt gacatcggtg
     1921 ccaatgaagt tcccttgcac cacgttcccg gttgccctgt tcccggctat gaaaacgcct
     1981 gactggccat tgccggaaat gatgttgacc gttccggcgg tcgttccccc gacggtgttg
     2041 tcagacgcga agccgatgat caccccgtcg aggccgttgc ccaaacgggc aacaccccta
     2101 acgtcggtgc caatgaagtt gccttgcacg aggtttttcg tggcactgga gccctgaata
     2161 cgaacccccc acatgccatt gcctgatata atgttgcgct ccgcagccgc agttcccccg
     2221 atgatgttat cgggagaatt gttgatgtga actccagatt ggccgttgcc cagagcaatg
     2281 ccgccggtaa cgtctgtgcc gatgaagttg ctcacgagta cgttgccacc actggtcacc
     2341 aaccctattc cattattccc aaaacgatta atcaccaacc cctttaccgt actgcctccc
     2401 gcgctgatta ccaggccatt ggcaaatccg gcaccgcttc cgttcagctc tatccggtta
     2461 cccgtcggct gcgtggtgcc gtctatcacc actgcttccg tgatagtcgg cagggcactg
     2521 ccggggctga tcgttggcac ccccacggcg gcgacgttga agttgatgct atccacgccg
     2581 gggtgcgcgt tggcctcctc aagcgccgcc cgcaaagtgc attgaaaacc gagcgtaatc
     2641 ggatcgatgt cgcagccgcc ggaaccggga gcggaatcgc tctcgtcccc tgtcaagttg
     2701 acggtgaaag tggtggcggc tccggattcc cggccctcgc tctggatccg attcacccgg
     2761 gcgtaggtgg cgaaaaacga taaaagggcg atgacgactg aaacgccgaa gaatttcaat
     2821 ttgctcactt cccaacgatt gcgcatgcta cctcccgcgc ccgcacgact cttggagaac
     2881 gagtcgatcc caccagctct tgaggagaag tgagcggcgg cactgtgccg ccccggactc
     2941 ctttctgctg cggcggcgag cacccgccct tcaccctcgc tggtggtaag ataagaggac
     3001 taatttactt atcaaaagaa aactatgcgc actgcggttc acaccatctg aggcgagctc
     3061 gccgtgcctt ccacttacag gcagcaggga gttggactcc cgaacactac gaactgtatc
     3121 agaaggacga tgtccactat gtcccgcaca tcgtcgcagt tggtgtcccc ggcgtgagga
     3181 ttggtgaggg gcgtgccgaa cacaatctct tgaatcaggg cgacaatatc gacaatgtcg
     3241 gccaccgcat cgtcggtgac atccccccgc actcctgcgc agacggctgt gccctcgttc
     3301 gacggcggcc cttcgttgcc ggagaaatcg accgcggtca cccggtagta gtaggttttg
     3361 gaggcatccg ccggagaatc gactgcgccc gtgtcgctcg tggccttaat gcggttggcc
     3421 ggtgaaaggg taaaaccggc caccgtgtcg cggtagagga agaagaagtc aaagtcgaaa
     3481 tccggaactg gacgccaagc caggacgatg ccgcccggtg tgccggaggc ggagagattg
     3541 gccggcggcg ccgggacgag gttgtcaatt gagtagccgg agtccaaagg cgagtcaatc
     3601 gacaggagag gattagttga gcggccgcgc acaaagaaga ccgaatactt tgtcccggcc
     3661 agggtggaat caaccagcgt cggtacgata gtcgcatagg ccgtatcacc ggtggccgcc
     3721 agaaccgcta cctgcagcca gtcgcccggc ggcaggggaa gggcatcagc cgccagcaca
     3781 ccttttccgg gcgagttgaa cgtcgaatcg aggcggcgat aaacgataaa taagtccata
     3841 agcgagtcgt ttccgggaaa acttttccag cgaaaaatca cctgtttccc ctggtcgttg
     3901 gggacatcgg ccagggaggc gatccgcacc gaacgaagtg catacacgaa agccgaacca
     3961 gccgccgaga gtgcgccggg atcggcaaag ggggcgccga cgatgacctc ggccctgccg
     4021 tcaccgttca cgtccccggc gccggcgacc gagaagccca gggagtcgcc agccgcaccg
     4081 ttcctttgga agagcagtcc gccggtcgcc ccggaataga tgaaaaccga tccggcgtcg
     4141 gcgagccctc cgggatcggc tttgaaagcg ccgacgatga actccgcttt cccgtcacca
     4201 ttgacgtcac ccgcacccgc cgcccagaag ccgaaccggt cttcaacggc agcgccgtct
     4261 ttctgataga gcaatgttcc cgttgccccg gagtaaataa agaccgaacc ggcattgact
     4321 accgcaccgg gatcggccag ccgcgcgctg atcagaaaat cgccgcgccc gtcgccatcg
     4381 acgtctcccg cacccgatac gggccagccc agttcatcgc cggtgacggc accgtctttc
     4441 cggtagagaa gcgccccggt cgctccggag taaacgaagg ccgacccggc atcaatgagc
     4501 ccgagggtcg gatcggccag gcgcgcgccg atgatgaaat cggcgcggtt gtcgccattc
     4561 gcgtctcccg caccggctac cgaaacgccg aattgatccc cctccgcacc gtccttctgg
     4621 tacaggagtg tcccggtagc accggagtag acgtaggccg agccggcatc gaggaggccg
     4681 cccggatcgg cgaagtgcgc gccgacgatg aattcagacc ggttgtcgcc tgtggcatct
     4741 ccgactcccc cgacagaaaa accaaagaga tcgaacgccg ccgccccatt cttctggaaa
     4801 agcagcgccc ccgtcgcgcc ggagtaaacg aaggccgaac cggcgaaccc gactccgccc
     4861 ggatcggcaa agggagcgcc aacgatgaaa tcggctttcc cgtcgccgtc cacgtcaccc
     4921 gctccggcga ccgactggcc gaactgatca cccgccgtgg ggccgttttt ctggtacaag
     4981 agcaaaccgg tggcaccgga gtaaacgaaa gccgacccgg cattggcgag cccgcccgga
     5041 tcggcaaagg gggcgccgac gatgaaatca cttctcccgt cgccgttgac atcgcccgct
     5101 ccggccacgg tatagccgaa ctggtcagcg gccgcagctc cgtttttctg gtagagcaga
     5161 agctccggcc agtcgaaccc gtagacggtc ggcgttcgag aggccaacaa aaaaatcacg
     5221 actgtgaaga accggtacgc tttctgcata tagttactca aaagcgcgag tcccctccct
     5281 gttcctcgct ggtgtttaat ataatgcccc ctggaggttg tcaagcaatt ttgtctgttt
     5341 gtggcttaac taatccctaa ttcctggtct ctaatccctg ccttttagag gcagcacggg
     5401 ttcggactcc cgaacacgat atactgcacc agaagcgcca gatcgactat gtcccgcacc
     5461 gtgtcgcagt tcgcatcccc ggcggcggga tcggccaaag gcacgccgaa cacgatgtcc
     5521 tgcaccagcg cggccaggtc caggatgtct atgacgccat ctgtggtcac atccccgcgc
     5581 agtccggcgc agacggtgag gcactggcag aactccgaag tgttccccgc cgaatgggtc
     5641 gccgtggccg ttatgaattt accgccggga acggcaactg gaagagtaac atttatgctg
     5701 gcgttgccgc tgccatcggt caaaacgctc gtcgagccaa caaaagtctg cccttcccca
     5761 tagccggaag cgtcgcagga atcattggag aaaaactcca gccggaaact atcgttggcc
     5821 gtactgttta gcgtcccctc aatggtagtg ctacctccta cgcttaaggc tgaagtcaaa
     5881 accggaaagt tctgcagatt gttcgctccg gcgtcggcat ccccggtgtc attctgcgtc
     5941 actccatccg ctcccaaatc aattcccaac aggccgttaa aaaaaatggt gttgcggtgg
     6001 ctgctgttgc cggtgccagg ttggaaaccc atacccaccc cctggcttcc gttgaaggca
     6061 atggtgttgg ccgcaccccc tatctcgccc ccaatggtgt tgttgctggg ggcgagctcc
     6121 agccaaacac ccgcgtcccc gttgcccaat gcggcggttc cggaaatgtc ggttccaatc
     6181 aagttgccct gcacaaaatt cccggtcgca ttctggaaga aaacaccatt ggtatggttg
     6241 cccgaaatga ggttacgcgc tccagccgtc gtccccccaa ccgtgtttcc ggacgagttg
     6301 aagaaagaga cgccgctgaa ggtgtttccc aaagggacgg tgccgctgac gtcgctgccg
     6361 atgaagttgc cttgcaccac cgtgccggtc acgccgttta ccattttgac gccgtcgtcg
     6421 ccattgcccg aaatgacgtt gcgcgcccca gcggccgcgc ctccaataat gctgttggat
     6481 gaaccgtcga tgaatacgcc atccgcaatg ttgcctacgc cagccgtgcc ggagacgtcg
     6541 gtgccaatga agttgcccaa aatttggttg ccggatgcgc cgagagttat accgaaggaa
     6601 ttcgccgcga tgacgttgcg cgctcccgct accgttccgc cgacggtgtt gttggaagcc
     6661 gaattgcgga tgaacactcc ggagcgagca ttgcctaacg gggcggtgcc ggtagcgcgg
     6721 gtgccgatga agttgccctg cacgagattg ccggtggcgg cggcgttctc gaccaggatg
     6781 cctctgccgt tggcggagat tacgtttcct gccccggcgg cggttccccc gatgacgtta
     6841 ttcggtgcat cgtcaatatg cacgccattg ctatcgttcc ccaagcaaaa actgccggtt
     6901 gcgtcggttc cgatgaaatt gcccaacacc tggtttcccg tcgtgctact ccccgaaatc
     6961 tcgacaccgt ctttgttgtt c
//
LOCUS       DBNGZA010000262         1286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_365901, whole genome shotgun sequence.
ACCESSION   DBNGZA010000262 DBNGZA010000000
VERSION     DBNGZA010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_365901"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..542
                     /locus_tag="AC16FU_05520"
     CDS             183..542
                     /locus_tag="AC16FU_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR2993865.1"
                     /translation="MAEAAVKERPQLKIELTQAAIVQLKRHLAGQPDPNTKVRVGVEG
                     GGCSGLQYILKFDPQTNQHDKVFDFEGVPVVVDLRSLLYLNGTVLDYTGDLLGGGFKF
                     NNPTAKRSCGCGTSFSV"
     gene            629..1282
                     /locus_tag="AC16FU_05525"
     CDS             629..1282
                     /locus_tag="AC16FU_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HIR2993866.1"
                     /translation="MEIFEIERFARDGVLEEESFRNALDQLDWSKYGGREVLIKGCAE
                     ILIPTWAYVVVAARLANHAKKIYYGEVQKPIPVYEQGNGSRPAATPSTPITRPSGPVR
                     VTFQPANKEVTAQPGQTILEIATDNDIVLEYECGGNCQCTTCHVIVEEGIEHLSPKEE
                     AEADMLASAEGLTEKSRLGCQARVYGDIVAVIPERDPFKLDSLLKEMGVDPKDLPPL"
BASE COUNT          310 a          336 c          395 g          245 t
ORIGIN      
        1 atgacctttc ctgtctgcgg agcgaaagcc gggggtgcaa ccgcatcgac tgctgtatga
       61 ccaggcggcc gaccttgaag gcgcaggaga aagtcgccca agtgctggag gagatgacac
      121 tggacgatat ctcatagcgg tgttcggcaa gtttgtgaac agctaaggga aggagagata
      181 gcatggcaga agcggcggta aaagaacggc cccaactgaa aattgagtta acgcaagcgg
      241 caattgtaca gttgaagcgg catctggccg gccaacccga tccgaacacc aaagtccgcg
      301 tgggtgtgga aggcggcggc tgctccggct tacagtacat cctgaaattt gacccccaga
      361 ccaatcagca cgacaaggtg ttcgacttcg agggggtgcc ggtggtcgtt gacctgcgca
      421 gtttactcta cctgaacggt acggtactcg actacacagg tgatcttttg ggcggggggt
      481 tcaagttcaa caaccccaca gccaagcggt cgtgcggctg cgggacgtcg ttttcggtgt
      541 aaggggaatt tccgcgatat tgggcgaccg gaggcggcca tacggcccgt aagggttgcc
      601 ccacatcggt ggtacggagc agaattaaat ggaaattttc gaaattgaac ggttcgccag
      661 agatggggtg ctggaggaag aatcgttccg taatgcctta gaccaacttg actggtcaaa
      721 atacggcggg cgggaggtgc tgatcaaggg ttgcgccgag attctcatcc cgacgtgggc
      781 gtatgtcgtc gtggccgcgc ggctggccaa tcacgccaaa aagatttact acggcgaggt
      841 gcaaaaaccg attccagttt atgaacaggg gaacggctcc agacccgctg ccactccttc
      901 caccccgatc acccgcccct ccggtccggt tcgcgtgacg ttccaaccgg ccaataaaga
      961 agtcaccgca cagccaggcc agacgatttt agaaattgcc accgataatg acattgtctt
     1021 ggaatatgaa tgcggcggga actgccagtg caccacctgc cacgtgatcg tcgaagaagg
     1081 aattgaacat ctctccccta aagaggaagc ggaagcggac atgctcgcca gcgccgaggg
     1141 gctgactgaa aagtcgcggc tgggctgcca ggccagagtg tacggcgaca tcgtcgcggt
     1201 gattccggag cgggaccctt tcaagctcga ttccctgctc aaagagatgg gggttgatcc
     1261 caaagatttg ccgccgctgt gaaaaa
//
LOCUS       DBNGZA010000263         1753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1743235, whole genome shotgun sequence.
ACCESSION   DBNGZA010000263 DBNGZA010000000
VERSION     DBNGZA010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1743235"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /gene="gltX"
                     /locus_tag="AC16FU_05530"
     CDS             complement(<1..874)
                     /gene="gltX"
                     /locus_tag="AC16FU_05530"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR2993867.1"
                     /translation="MKSVRVRIAPSPSGFLHVGTARTAVYNYLFSRHHGGAFILRVED
                     TDVARSSPEMVAGILEGLQWLGLTWDEGPVFQSRRFDLYRQYAEKLFATGQAYFCFCT
                     PEELEQKRSAAIAQKIAWKYDRTCRQLSDAERQKKLAAGVPKAMRLFVPEGETVFRDS
                     VYGELRRHNADTEDFVCLRSNGRPTYNFACVADDADMQISHVIRGNDHISNTFKQILL
                     YRALGLEPPEFAHLPLILGPDRAKVSKRHGAESVTEFRDRGFLPETVLNFLALLGWSP
                     GDEREILSRKELIDL"
     gene            complement(871..1287)
                     /locus_tag="AC16FU_05535"
     CDS             complement(871..1287)
                     /locus_tag="AC16FU_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993868.1"
                     /translation="MRYEIKRLGFWSVIKVTFILYGILGFLLGIFYALFLAFFASLAE
                     YAGGQEFPGIFSGALLVFVPIFVAFIVAIGGVIQNGVLVGLYNLFVRAIGGIEVTVAA
                     LEPTPVVPTAGTAPVTYTFTPTQPPQPGQPPPMSAV"
     gene            1469..>1753
                     /locus_tag="AC16FU_05540"
     CDS             1469..>1753
                     /locus_tag="AC16FU_05540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR2993869.1"
                     /translation="MGERGRKFVLIGFFIVIGLLANTGIAKVMTSTVSYPSGSDTVSG
                     YLALPGGQGPFPALVVIQEWWGLNDWIKENARKFAEQGYVALAVDLYRGRV"
BASE COUNT          392 a          490 c          522 g          349 t
ORIGIN      
        1 aaagatcgat taattctttc cgggaaagaa tctccctctc gtccccgggc gaccagccta
       61 acagcgccaa gaaattcaaa accgtttcgg gcaaaaagcc ccggtcgcgg aactcggtga
      121 ccgactcagc cccatgcctt ttggaaactt tggcccgatc cggccccaaa atgagcggca
      181 ggtgagcgaa ctccggcggc tccaaaccca gcgcccggta gagcaaaatc tgtttgaagg
      241 tgttggaaat gtggtcgttc ccccggatga cgtgcgaaat ctgcatgtcg gcatcgtccg
      301 ccacgcaggc gaagttgtag gtcggccttc cgttggagcg caggcagacg aaatcttcgg
      361 tgtcggcatt gtgacgccgc agttcgccgt agactgaatc gcggaaaacg gtctcccctt
      421 ccggcacaaa gagccgcatc gctttcggaa ctccagcggc taatttcttt tgccgctcgg
      481 cgtcgctcaa ctgccggcag gtgcggtcgt atttccaggc gattttttgg gcgatggcgg
      541 cgctgcgctt ttgctccaat tcctccggcg tgcagaagca aaagtaggcc tggccggtgg
      601 caaataattt ctccgcatac tggcggtaca ggtcgaaccg ccgggactgg aagaccggcc
      661 cttcgtccca ggtcaagccc agccactgga ggccttccaa aattcccgcc accatttcgg
      721 gcgaagagcg ggccacgtcg gtgtcttcga cgcgcaaaat gaaggcgccg ccgtggtgcc
      781 gggagaaaag atagttgtaa acggcggtgc gggccgtgcc cacgtgcaaa aagccggaag
      841 gcgacggcgc aatgcggacg cgaacggact tcatacggca gacattggcg ggggctggcc
      901 gggctgaggc ggctgcgtcg gcgtaaaggt gtaagtgacc ggggcagtac cggccgttgg
      961 gacaaccgga gtcggttcca gagcggctac ggtcacctcg atgccgccga tggcgcgcac
     1021 aaagagattg tagagtccca ccagaacccc gttctgaatc acgcccccaa tggccacgat
     1081 gaaggcgaca aatatcggca cgaaaactaa aagcgccccg gagaaaatcc ccggaaattc
     1141 ctgccctccg gcatattccg ccagactggc gaagaatgcc agaaacagag cgtagaagat
     1201 gccgagcagg aatcccagaa tgccgtagag gatgaaggtc accttgatca ccgaccagaa
     1261 tcccaaccgt ttgatctcgt agcgcatcgt gtacccccga tctgacgttc actccgtttc
     1321 ggtcactgcc ggtcaagtat gcaccattgc agagattttg taaaggcgtg gctttcctca
     1381 ggttcggagc gacgcttgct tttatccccc gaaatctctt tttttctgac gtagtaagaa
     1441 taaatgggta tacaaccggg gaggaaaaat gggagagcgc ggtagaaaat ttgtcttgat
     1501 cggttttttc attgtcattg gacttctggc gaacacgggg attgccaaag ttatgaccag
     1561 cacggtctct taccccagcg gcagcgacac ggtttcgggc tacttggcac tgcccggggg
     1621 ccagggccct tttccggctc tggtggtgat ccaagaatgg tggggcttga acgactggat
     1681 caaggagaat gcgcgaaaat tcgccgagca gggctacgtg gccctggctg ttgatctcta
     1741 ccggggcaga gtt
//
LOCUS       DBNGZA010000264         7278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_473025, whole genome shotgun sequence.
ACCESSION   DBNGZA010000264 DBNGZA010000000
VERSION     DBNGZA010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_473025"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1866)
                     /locus_tag="AC16FU_05545"
     CDS             complement(58..1866)
                     /locus_tag="AC16FU_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2993870.1"
                     /translation="MMQTMRNITKMVMWVVLAGFVGLIVFQWGMQQQSASKMKGILGK
                     VNDVEIQPALYDYYYNRLYEQQKQQKGEVPEEQVPALRQQAWTGMVNDLLMQGEVKKF
                     NLRVTDPELVDFLKKFPPPELQKLPDFQTEGKFDYTKYLQIMANPAAAPLWSQVEDLV
                     RPRLLQIKTEQFILSPARLTDEEVQDYFRTQNEKVKVRYIFIPNREFEPQVASLSDAE
                     VEAFYAKNQERYRMVERGALKYVEWAKTPTAADTTRVTNQLEALRQQALAGADFAELA
                     REYSQDPSAAQNGGDLGWFGKGQMVGPFEQAAYALKPGEISPPIKSPYGYHIIKLHER
                     KTEKKEEKVHASHILLKVEVSSETLERLKREAEDFTAAARKVGFDRAAAEMKLEVKPT
                     PRFLKGGFISGLNSDNPQFHNFVFRNKVGEISDPIEIEQGFYVVMATERQPAGIPPLA
                     DIKPAVSRAAADEKYQHLTARKGLELYADLKKGMTFDQVAAKYSEKVEATDFFTIGTG
                     VPKVTADVELIGALKALSDSNRYSPPVPFLRGTMLFEFVAHQPADSLRFAQVKDSLYT
                     TLLQQKATNLYQVWLSLLKERATIEDYRAEVFGSNM"
     gene            2176..2433
                     /locus_tag="AC16FU_05550"
     CDS             2176..2433
                     /locus_tag="AC16FU_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HIR2993871.1"
                     /translation="MRKITREELKRCNGANGSPIYIAYKGKVYDVTLSPLFFDGVHFD
                     HFAGDDLTEVLQDAPHGEEVFDLYPCVGELTDEAPARTGGE"
     gene            2614..3648
                     /locus_tag="AC16FU_05555"
     CDS             2614..3648
                     /locus_tag="AC16FU_05555"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR2993872.1"
                     /translation="MRSVAAPPPPQAAREVKVQAYPGLTDKEKKDLYTLLVRCRTLDE
                     RMRKLFRAGRFAGTYFSGVGQEATTIGTAYNLRQQDVIAPAHREIGASVAKGVPTLQL
                     IAQAYARKNSVDKGKSHPCHYGWAPLNVINPASTIAGQTVVGTGCALAFKLRREDHVV
                     LAYCGEGATANGAWYEALNFAAIHKLGMIFVVQNNLWAESVPARLGAALEDYHHRADG
                     VGCPGVSIDGNDVFRVYEVAREAIARARKGLGPTVIECKTYRWYGHSEIDPADYRTQK
                     ELEYWQSLDPVPRAEKHLRELGILTDALKQKIISDLEKEVEEAVDICENTPYAEPEEA
                     LRDVYADSPK"
     gene            3761..4744
                     /locus_tag="AC16FU_05560"
     CDS             3761..4744
                     /locus_tag="AC16FU_05560"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR2993873.1"
                     /translation="MRQITYIEAITEAMTQEMERDPRVFVMGEDIGLYGGVFKATKGM
                     QKRFGEYRVLDTPIAENLIVGLGVGAAIQGMRPISEIQFSDFFTYSMDQTVQQAAKLR
                     YRSGGTWTVPMVIRVCCGGEVGGGLYHSQTNEAWYLHCPGLKVVVPGTPYDAKGLLLA
                     AIRDPDPVVYFEHKRLYRSLKADVPEGDYTVPIGKASVVRAGKDITIVAYQLMLQKSL
                     EAAQLAAKEGIDVEVIDLRTLLPIDKATILESVKKTGRVLLVQEAPKTGGAAAELSAI
                     ISEEAFDFLDAPIVRLAGLDIPPIPFAPPLEHFYLPNAQKILAKLRELKAY"
     gene            4856..6124
                     /gene="sucB"
                     /locus_tag="AC16FU_05565"
     CDS             4856..6124
                     /gene="sucB"
                     /locus_tag="AC16FU_05565"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR2993874.1"
                     /translation="MAATVVIPQMGESVVEATISKWLKKEGDPVKRDEPLVEIMTDKI
                     NAEIPAHKAGVMGKILVPEGTTVPIGSQIAWILDPGEKMPTDGKGADSVQLTPTAAPA
                     KPQAAPAPAAAAVEAEERESIRTSPAVRRLAKEFDVDLAQVRGTGGGGRITKEDVEAY
                     VAARKKGPAAPAAPKAAPPTAPRPIVAGPREEIVPFAGVRKVIADHMIKSKHTAAHYT
                     TFDEADMSRLVELRTKYKEPIKAKYGFNLTYMPFLIKASCMALKEFPVVNASINEDKI
                     IYKKYYNIGVAVGRDSGLIVVVVKDADQKGILEIAREVNELSAKAHADKLAIDDITGS
                     TFTITNAGMFGALGSTPIIHYPEVAILGVHMIQKRPVVRENQIVARDMMTLTLSLDHR
                     LVDGHIGVQFLRKLITYLEDPVMLFTTDWL"
     gene            6185..>7278
                     /locus_tag="AC16FU_05570"
     CDS             6185..>7278
                     /locus_tag="AC16FU_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993875.1"
                     /translation="MATKNQENGVTTLETDRVGMGNGKKRKMVSPGNRKGTRTRTVRS
                     FPASTFEEALVIPQAIQQFAAGQKVRRLTLFDHLKRSPDSGPSRQLVTNSSKYGLTDG
                     GYQAEHLDLTPEGKIATSQEVSERERVSARFKLAVEKIPPFNALYEKFKGNKLPAVAV
                     MRDFLLEEGYSKDDVAACVDTFILNAKFLGLLRTVAGAERLLSIEHVLEETPKESTTD
                     RQFHARVDGALQASVITEVGEKIDWSKICFYITPIGDEDSEQRKHADLFLSSIVEPAL
                     EEFGLKVVRADQIGKPGMITAQVIEHVVKSKLVIVDLSFHNPNVFYELCLRHVTRLPV
                     VQVIRSSDRIPFDLEQFRTIKIDTTNIYTLV"
BASE COUNT         1667 a         1965 c         2181 g         1465 t
ORIGIN      
        1 gagattgacc cctcccagac cctcccctca aggggaggga aatgttctat agtttgtcta
       61 catattgctc ccaaacactt cggcgcggta gtcttcgata gtggcgcgct ctttcaacag
      121 actgagccag acctggtaga gattggtcgc cttctgctgg aggagcgtcg tatacaatga
      181 gtcttttacc tgcgcgaatc ggagcgagtc cgccggctgg tgagcgacga actcaaagag
      241 catggtgccc cgcaaaaacg gcactggtgg ggagtagcga ttcgaatcgc tcagggcctt
      301 gagggcgccg atcagttcga cgtcggcggt caccttcggg acgcccgtcc cgatagtgaa
      361 gaagtcggtc gcttccactt tttcgctgta tttagcggcc acctgatcga aggtcattcc
      421 cttcttcaag tcggcgtaca gttccagccc tttgcgcgca gtgaggtgct ggtacttttc
      481 atcggcggcg gcgcgggaga cagccggttt gatgtcggct aaaggcggaa tgccggccgg
      541 ctggcgctcg gtcgccataa ccacgtagaa gccctgttcg atctcgatgg ggtcggaaat
      601 ctcgcccacc ttattccgga agacgaagtt gtgaaattgc gggttgtcgc tattcaagcc
      661 gctgatgaac ccgcctttca ggaagcgcgg ggtcggtttg acttccaact tcatttcggc
      721 ggcggcgcgg tcgaagccca ctttgcgggc ggcggcagta aaatcttccg cctcgcgctt
      781 gagacgctcc agcgtttcgg aggagacctc gactttcaac aggatgtggc tggcgtggac
      841 tttttcctct ttcttttcag tcttccgctc gtgcagtttg ataatatggt aaccgtaggg
      901 cgacttgatg ggcggcgaaa tttcccccgg tttgagagcg taggcggcct gttcgaacgg
      961 ccccaccatc tgccccttgc cgaaccagcc cagatcaccg ccgttctgag cggccgaggg
     1021 atcctgggaa tactcacggg cgagttcggc gaagtcggcc ccggctaagg cctgctggcg
     1081 caaggcctcc agctggtttg tcacccgggt cgtgtcggcg gcggtcggcg tcttggccca
     1141 ctccacatac ttgagcgccc cgcgttcaac catccggtag cgctcctggt tcttggcgta
     1201 gaaggcctcc acctcggcgt cggacagact cgccacctgg ggttcgaact cccggttggg
     1261 gatgaagatg tagcgcacct tcactttttc gttctgggtg cggaagtagt cctgcacttc
     1321 ttcgtcggtg aggcgggccg gggagaggat gaactgttcg gttttgatct gcaagaggcg
     1381 cggccggacc agatcttcca cctggctcca gaggggcgcg gcggcggggt tggccatgat
     1441 ctgcaggtac ttggtgtagt cgaacttgcc ctcggtctgg aagtcgggaa gtttctgcag
     1501 ttccggcggg gggaattttt tcaggaaatc gaccagttcg gggtcggtca ccctgaggtt
     1561 gaatttcttc acctcgccct gcatcaaaag gtcgttgacc ataccggtcc aggcctgctg
     1621 gcgcagggcc ggcacctgct cttcggggac ctctcctttt tgctgcttct gctgttcgta
     1681 cagccgattg tagtagtagt cgtagagcgc cggctggatt tcgacgtcgt tgaccttgcc
     1741 caggatgccc ttcatcttgg aggcggactg ctgctgcatg ccccactgga agacaatcaa
     1801 gccgacgaaa cccgcaagga ccacccacat caccatcttg gtgatgttgc gcatcgtttg
     1861 catcatacgg tgttactcct catcgatgct tttccaagac caataaccag tcgggctcga
     1921 gacgagcccg actaccgacc caagaacggc gacggctcat tgagagccgc cgaggttata
     1981 aacctcggct acgagtctac cgactgaggg gaatatacgg aagagaaagc cacaaacaag
     2041 tggagaagtt gggggggtaa gttttctgac gggagcgaac cctgtccccg gttcgggcag
     2101 cgcggccccg tacggttgac attcggtggc cggggtctac attctggcac gacgggcgga
     2161 ggatggcgac ggaacttgag gaaaattacc cgggaagaac tgaaacggtg caacggggcc
     2221 aacggcagcc cgatctatat cgcctacaag gggaaagtct acgacgtcac cttgagtcct
     2281 ttgttcttcg acggcgtgca ttttgaccac ttcgccgggg atgacctgac cgaagtgcta
     2341 caggatgcgc cgcacggcga ggaagtgttt gacctctacc cctgcgtggg ggagttgacc
     2401 gacgaggcgc cggctcgcac cggaggagag tagaaccgtg ccgcatctcc tgcctgtctg
     2461 cgcaccactt gccaaccgcc gaatttcgta ttaattgtac agtttccgac ccgcaggagt
     2521 ttttgaccag tggcggatcg acgtacgcga aggagtcaaa aacccgccag aagcgggaca
     2581 aaaaaggtaa tttttcacgg aggcagaaaa acaatgcgct cagttgccgc gcctcccccg
     2641 ccgcaggcgg cgcgggaggt aaaagttcaa gcgtacccgg gattaacgga caaagagaaa
     2701 aaagatctct acacccttct cgtccgctgc cggaccttag acgaacggat gcggaagttg
     2761 ttccgggccg ggcgcttcgc ggggacctat ttctccggcg tgggacagga ggcgaccacc
     2821 atcggcaccg cctacaacct ccggcagcaa gatgtaatcg ccccggcgca ccgggagatc
     2881 ggcgcctcgg tggccaaggg agtgccgacc ctgcaactca tcgcccaggc ctacgcccgg
     2941 aaaaactccg tcgacaaagg caaatcccac ccctgccact acggctgggc gccgctcaac
     3001 gtcatcaatc ccgcctcaac catcgctggg cagaccgtgg tgggcaccgg ctgcgcttta
     3061 gccttcaaac tgcgccggga ggatcacgtg gtgctggcct attgcggcga aggagccacg
     3121 gccaacggcg cctggtacga agcgctcaat tttgccgcca ttcacaaatt ggggatgatc
     3181 ttcgtcgtgc agaataacct ctgggcggaa tcggtcccgg cccggctggg tgcggcgttg
     3241 gaagattacc accaccgggc ggacggcgtc ggttgtccgg gggtgagcat cgacgggaac
     3301 gacgtgttcc gggtatacga agtggcgcgg gaagcgatcg cccgggcgcg caaaggcctg
     3361 gggccgacgg tgatcgagtg caaaacctac cgctggtacg gccactccga aatcgatccg
     3421 gccgactacc ggacgcagaa agagttagaa tactggcagt ctctggaccc ggtgccgcgg
     3481 gccgagaaac acctgcggga attgggaatc ctgaccgacg ccctaaaaca gaaaatcatc
     3541 agcgacctcg aaaaggaagt ggaagaagcg gtggatattt gcgaaaacac gccctacgcc
     3601 gagccggaag aggcgctgcg ggacgtttac gccgacagtc cgaaataaat ctcaccaccg
     3661 aggcacagtg gttcgattcg cctgaggcgg actcaccaca agatgacatg gagaaaagta
     3721 aatttccgat tgaaagtgaa aaacgcaaag ggataccgga atgcgacaga taacctacat
     3781 tgaagcgatt accgaggcga tgactcagga aatggagcgc gacccgcgcg tcttcgtcat
     3841 gggggaagac atcgggctct acggcggggt gttcaaagcc accaagggga tgcagaagcg
     3901 cttcggtgag taccgggtgc tggatacgcc gatcgccgaa aacttaatcg tcggcctggg
     3961 ggtgggcgcg gcgatccaag ggatgcggcc gatttccgaa atccaattct ccgacttctt
     4021 cacctactcg atggatcaga ccgtccagca ggcggccaaa ctgcgctacc gctccggcgg
     4081 gacctggacg gtgccgatgg tcatccgggt ctgctgcggc ggtgaagtgg gcggcgggtt
     4141 gtatcactcc caaaccaatg aagcctggta cctgcactgt cccgggctca aagtcgtcgt
     4201 ccccggtacg ccgtacgacg caaaaggatt gcttttggct gcgattcgcg atcccgatcc
     4261 ggtggtctac ttcgaacaca agcggctcta ccgctcactc aaagccgacg tgcccgaggg
     4321 tgactacacc gtgccgatcg gcaaggccag cgtcgtacga gcgggaaagg acatcaccat
     4381 cgtcgcctac cagttgatgc tgcagaagtc cttagaagcg gcgcagctgg cggccaagga
     4441 aggcattgat gttgaggtga tcgacctgcg gacgcttctg ccgattgaca aggctacgat
     4501 tctggaatca gtgaaaaaaa ccggccgggt gctcttagtt caggaagcgc ccaaaaccgg
     4561 cggcgcagcg gcggagcttt cggccatcat ttccgaagaa gcgtttgatt tcttagatgc
     4621 gccgatcgtg cgactggccg gattggacat cccgcccatc ccctttgcgc cgcccctgga
     4681 gcatttctac ctgcccaatg cgcagaagat tctggcgaag ttacgagagt tgaaagcgta
     4741 ctaaagcaga ggattgtggg acaattaaag ggcggcacta agagtgccgc ctacgcgtgt
     4801 cgggtaaaac ttcggtaatg gtgccttaat cttcaaggat agacggaggg tgtaaatggc
     4861 tgcgacagtg gtaataccgc aaatggggga gtcggtcgtc gaagcgacaa tttccaagtg
     4921 gctcaaaaag gaaggcgacc cggtgaagcg ggatgagccc ctggtggaaa tcatgaccga
     4981 caagatcaac gccgaaattc cggcgcacaa agccggggtg atgggcaaga ttctggtacc
     5041 ggagggaacg accgtgccca tcggttcgca gatcgcctgg attttggacc cgggcgaaaa
     5101 gatgccgacc gacggtaaag gtgctgacag cgtgcagttg accccgaccg ccgcgcccgc
     5161 taagccgcag gccgcaccgg ccccggcagc tgccgcagtt gaagcagaag aacgggagtc
     5221 aatccgcacg tcaccggcgg tgcgccggct ggcgaaagag ttcgacgtcg atctcgcgca
     5281 ggtgaggggc acaggcggcg gcgggcggat caccaaagag gatgtggaag cgtacgtcgc
     5341 ggcgcgcaag aaaggcccgg cagcaccggc cgctcccaaa gcggcgccgc cgaccgctcc
     5401 gcgtcccatt gtcgccgggc cacgcgaaga gattgttccc tttgccgggg tgcgcaaggt
     5461 gattgccgac catatgatca aatcgaagca cacggcggcg cactacacga ccttcgacga
     5521 agcggacatg tcgagactgg tcgaattgcg aacgaagtac aaggagccga tcaaagccaa
     5581 atacggcttc aacctgacct acatgccgtt tttgatcaag gcctcctgca tggcgttgaa
     5641 agaattcccc gtcgtcaacg cctcaatcaa cgaggacaag atcatttaca agaagtacta
     5701 caacatcggc gtggcggtgg ggcgggacag tgggctcatc gtggtggtgg tcaaagacgc
     5761 cgatcaaaag gggattctgg agatcgcccg cgaggtgaac gaactttcag ccaaagcgca
     5821 cgccgacaag ttggccatag acgacattac cgggtcgacc ttcaccatca ccaatgccgg
     5881 gatgttcggc gctctgggct cgacgccgat catccactac ccggaagtgg cgatcttggg
     5941 cgtccacatg atccagaagc ggccggtggt gcgggagaac cagatcgtcg cccgcgacat
     6001 gatgaccttg acgctctcgc tggaccaccg attggtggac ggccacatcg gggtgcagtt
     6061 tttgcggaaa ctgatcacct acctcgaaga cccggtgatg ctatttacga cggattggct
     6121 ataggaaaga agattgcgtt gaagatgttt aagaagtatt atgttagtac ggagggtaaa
     6181 tagaatggcc acaaaaaacc aagaaaacgg tgtgacgaca ctcgaaacag atcgtgtcgg
     6241 gatggggaat ggtaaaaagc gtaaaatggt atcccccggt aatcggaagg gaacacgtac
     6301 ccgaactgtt cggagttttc ctgcttcgac gtttgaggaa gcactggtta ttccccaagc
     6361 gattcagcaa ttcgcagcgg gtcaaaaggt gcgaagactc acactgtttg atcacctaaa
     6421 aaggtcacca gatagtggcc cgagcagaca actggtcacg aattcatcga aatatggatt
     6481 aactgatggt ggctatcaag ctgagcattt ggatctaaca ccggaaggta aaatcgcaac
     6541 cagccaagaa gtgtcagaaa gagagcgcgt gagcgctcga tttaaacttg cggttgaaaa
     6601 gatacccccg tttaacgcgc tttatgaaaa atttaagggt aacaaattac cggcagtggc
     6661 agtaatgcgg gactttttgc tcgaggaagg ttacagtaaa gacgatgtgg cggcgtgcgt
     6721 agataccttc atcctaaatg caaagtttct aggtcttctg cgtactgttg ctggtgcgga
     6781 aaggttgctt tcaattgaac atgttctaga agaaacaccg aaggaatcaa cgaccgatag
     6841 gcagttccat gcacgtgtag atggtgcctt gcaagcttca gtcattactg aggttggcga
     6901 aaagatagat tggtcgaaga tttgttttta tataacccca atcggagacg aagattccga
     6961 gcagcgcaaa cacgcagacc tttttctaag ctctatcgtt gagcctgcat tagaagagtt
     7021 tgggcttaag gtagtgcgtg cggatcaaat aggaaaaccc gggatgatta ccgcacaagt
     7081 aattgagcat gttgtgaagt ccaaacttgt cattgtggat ctatctttcc ataatccaaa
     7141 cgtcttctat gagctatgct tgaggcacgt aactcgtctt ccagtcgttc aagtcattcg
     7201 aagtagtgac cgtattccat ttgaccttga acaatttaga actattaaga tcgatacaac
     7261 taatatctac acgcttgt
//
LOCUS       DBNGZA010000265         3653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2486559, whole genome shotgun sequence.
ACCESSION   DBNGZA010000265 DBNGZA010000000
VERSION     DBNGZA010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2486559"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /locus_tag="AC16FU_05575"
     CDS             <1..2034
                     /locus_tag="AC16FU_05575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR2993876.1"
                     /translation="GVKDYLDMLLRVEREPGLKVTFNWVPSLLEQLDDYCSGRTSDPH
                     LDLTRVPAALLSPEQKLAIVTDFFLGPPETMIKPHRRFWSLWLKKERAAGDRDRARDS
                     FTEEDWRDLQVWSNLAWFDPLFRRGTPLTPLFEKGERFSEADKQVMLDYQFDILRQIV
                     PTLKRLRAAGQIEVSFSPAYHPILPLLVDSRCAREALPDATLPDPPFRHPEDAREQIR
                     SACVLYEELFGERLQGLWPPEGGVSADLVPLVTRQGIGWIATDQKIYERSVAAGGEPG
                     LGLYQPYRIGTGDQSLAVFFRDHTLSDLIGFAYARWDAERAVNDLLERLHAIRAHLPE
                     EALPRAAVSIILDGENAWEYYPEDGGRFLDLLDERLTGDPLLETITFGEYLRRHPDLP
                     ALSRLAAGSWIYGNFNVWIGQPEDNRAWEFLRAARALLQAKELAGAPAPQVEQARRTL
                     SRAEGSDWFWWYGEDHRTEQKDIFDELFRAHLCEVYRALETSPPPDLLLPIGGEPARR
                     SEPPSQVIHPTIDGQITHFYEWSGAGLYRVGAGSMRPAHAGVTRRIWYGLDETRLYLR
                     CDFAKGVPVERLRTLRFRFDFQTQPPLAIELSGDRLTILPEQPPGTALISGKVETILE
                     LALPLELLSVGADRRVPVSFSVYAEKVLLETWPAHGALAIEIPPEVATAFWQA"
     gene            2044..2397
                     /locus_tag="AC16FU_05580"
     CDS             2044..2397
                     /locus_tag="AC16FU_05580"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR2993877.1"
                     /translation="MTENPGTPESPSDKLAADATEEGKAAAVLAYIPFLCFVPLLKMR
                     DNPFARHHGKQGLLLFFVELLAVIFYLPVIAQHFWPFILILCFLAALVGVLEALQGRW
                     WKIPFLGDLADKIKL"
     gene            2409..3275
                     /locus_tag="AC16FU_05585"
     CDS             2409..3275
                     /locus_tag="AC16FU_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR2993878.1"
                     /translation="MRSYRFVTSLGVLDRGRWKAYVCGMRTFGTTLLLSVILLSGCAT
                     TGPGGKKSLILIGSDSEVSFGKQMAEQVGSESKVFPDTTWARYVNAVGQGLVAVCDRK
                     NIAYSFTVLEKDEINAFALPGGYLYIYTGLLKLMDSEAELAAVLGHELSHVVARHGIK
                     RMQQVLGVSLIEQIALGSRGSKAAKSAINLGIGIALQGYSRENELEADHDGVYYLHRA
                     GYDPKAAVTMFQKLAGEEKGGRSVFERLAASHPETKERIARVEKEIESFAPRANPTLG
                     KEPYQELKRRLP"
     gene            3290..>3653
                     /locus_tag="AC16FU_05590"
     CDS             3290..>3653
                     /locus_tag="AC16FU_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993879.1"
                     /translation="MRNFWYSLGSGCGPATVTAVSALFLLAGLSAPLSAGKAKTGTFQ
                     KGLYSDARFGFHLTIPDSWKVQLKDDPALVRIIVTKRHPEIDPRLRNTPESATIPTIL
                     VLADTTALDLDTVKVWLWA"
BASE COUNT          707 a         1105 c         1101 g          740 t
ORIGIN      
        1 ggtgtaaaag actaccttga tatgctcctg cgggtcgaac gagagccggg tttgaaggtg
       61 accttcaact gggtcccgtc tcttttggaa cagctcgacg attactgttc cggaaggaca
      121 tccgaccctc atctggactt gacccgtgta ccggcagccc tactttcccc cgaacagaag
      181 ctggcaattg tgaccgactt cttcctcgga ccgccggaga cgatgatcaa gccacaccgg
      241 cgcttctggt cgctgtggct caaaaaagag cgtgccgcgg gtgatcggga tcgggcgcgg
      301 gactctttca ccgaagaaga ctggcgcgac ctgcaggtct ggagcaacct cgcctggttt
      361 gatcccttat ttcgccgggg tacaccgttg acgcctcttt tcgagaaagg tgagcgtttc
      421 agcgaagccg acaaacaggt gatgctcgat tatcagttcg acatcttgcg gcagattgtg
      481 cccacgctca agcgcttgcg tgccgccggg cagattgaag tttctttttc gcccgcttat
      541 cacccgatct tgccgctttt ggtggactcc cgctgcgccc gagaagcgct gccggatgcg
      601 acactgcccg atcccccttt ccggcatccg gaagatgcga gagagcaaat ccggtcggct
      661 tgcgtgctct atgaagaact cttcggtgaa cgcctccagg gcctctggcc gccggaggga
      721 ggcgtctccg ccgatctggt tccgctcgtt acccgacagg gaatcggctg gatcgctact
      781 gatcaaaaga tttatgagcg ctcggtcgca gccggcggag aacccggatt gggactatac
      841 caaccctacc ggatcgggac gggtgaccag agcctggccg tcttcttccg tgaccacacc
      901 ctgtccgatc ttatcggttt cgcctacgcc cgctgggatg cggagcgcgc ggtcaatgac
      961 ttgctggagc gtttgcacgc catccgggct cacctgccgg aagaggcatt gccgcgggct
     1021 gcggtctcga tcatcctgga cggcgaaaat gcctgggagt actatccgga ggacggcggg
     1081 cggtttctcg atttgctcga cgaacggctg accggcgatc cgcttttgga aacgattacg
     1141 ttcggagagt acttgcgccg ccatcccgac ctgccagccc tctcgcgact ggccgccgga
     1201 tcgtggatct acggaaattt taacgtctgg atcggccagc ccgaggacaa ccgggcctgg
     1261 gagtttttgc gcgcggctcg ggcgctcttg caggcgaaag aactggccgg ggctcccgct
     1321 ccgcaggtgg agcaagcgcg ccgcacgctg tcccgagccg aaggcagcga ctggttctgg
     1381 tggtacggcg aagaccaccg cacggaacag aaagacattt tcgatgaact cttccgtgcc
     1441 cacctgtgcg aagtgtaccg ggcgctggaa acctcgccgc cgccggatct tttgcttcct
     1501 atcggcggcg agcccgcgcg tcggagcgag ccgccgtcgc aggtcattca tcccacgatt
     1561 gacggtcaga tcacccattt ctacgagtgg agcggcgccg gcctctaccg tgtcggcgcc
     1621 gggagcatgc gccccgcgca cgccggggtg acgaggcgca tctggtacgg cctggatgaa
     1681 accaggctct acctccgctg cgattttgcc aaaggtgttc cggtggaacg cctccgaacc
     1741 ctgcgtttcc gcttcgactt ccaaacgcag cccccgctgg ccatagaact atccggcgac
     1801 cggctcacca tcctgcccga acaaccgccg gggaccgctt tgatctccgg gaaagtcgaa
     1861 accatattgg aactcgctct tccgttggaa cttttgagcg tcggcgcgga ccgtagggtg
     1921 ccggtgagct tttcggttta tgccgaaaag gtgctactgg aaacctggcc ggcccacggc
     1981 gccctggcca ttgaaatccc gccagaggtt gccaccgctt tctggcaagc ctgagaggtc
     2041 gaaatgacgg aaaaccccgg gacacccgaa tccccatcgg acaaactggc tgccgacgca
     2101 accgaagagg gcaaagccgc ggcggttttg gcctacatcc ccttcctctg ctttgtcccg
     2161 ctcctcaaaa tgcgcgacaa ccctttcgcg cggcaccacg gtaagcaggg gcttttgctc
     2221 tttttcgtcg aattgcttgc ggtcatcttc tacctgccgg tgattgccca gcatttctgg
     2281 cccttcattt tgatcctctg tttcctggcg gcgctcgtgg gcgtgctgga agcgcttcag
     2341 ggacgctggt ggaagatccc gtttctgggt gacctagcgg ataagatcaa gctgtgagtc
     2401 ggaagcccat gaggtcgtac cggttcgtca cgagtctcgg cgtgcttgat cgcgggcggt
     2461 ggaaggccta tgtttgcggc atgcgcacct tcgggacgac cttgctttta tcggtcatcc
     2521 tcttgtccgg ctgcgccacc accggcccgg gcggcaagaa gagtctcatc ctcatcggca
     2581 gcgattcgga ggtctctttc ggcaagcaaa tggccgaaca ggtggggtcg gaatcaaaag
     2641 tatttccgga caccacgtgg gcccgctacg tcaatgccgt cggccaaggt ctggtggcgg
     2701 tctgcgaccg gaagaacatt gcctactcgt tcaccgtgct ggaaaaagac gaaatcaacg
     2761 cctttgctct tcccggcggc tatctctaca tttataccgg acttttgaaa ttgatggaca
     2821 gcgaagccga actggcggcg gtgctgggtc acgaattgag ccacgtggtg gcccgccacg
     2881 gcatcaaacg gatgcagcag gtgctgggag tctcgctcat cgagcaaatt gcgctgggca
     2941 gccgcgggag caaggcggcc aaaagcgcga ttaacctggg cattgggata gcgctccagg
     3001 gctacagccg ggagaacgaa ctggaagctg atcatgacgg cgtttactac ctgcaccggg
     3061 ccggctatga ccccaaggcc gcggtgacga tgtttcagaa actggccgga gaggaaaaag
     3121 gcggccggtc agtttttgaa cgcctggccg ccagccaccc cgagacgaaa gaaaggatcg
     3181 cccgcgtgga aaaagagatc gaaagtttcg ccccgcgcgc gaacccaacc ctggggaaag
     3241 aaccgtacca ggaattgaag cggcgtctcc cgtgaagaga gaacagacga tgcgaaactt
     3301 ttggtactcg ttaggctctg gctgcggtcc ggcgacggta accgccgtca gcgctctctt
     3361 ccttttggcg ggattgagcg ctccgctatc ggcggggaaa gcgaaaaccg gaactttcca
     3421 gaaggggctc tacagcgacg ctcgtttcgg cttccacctc accattccgg acagctggaa
     3481 agtccagctc aaggacgatc cggcgctcgt acgcatcatc gtaaccaagc ggcacccgga
     3541 aattgacccg cgcttacgca acacgccgga gagcgccacg attccaacga ttttggtttt
     3601 ggctgatact accgcgttgg atctcgacac tgtcaaagtc tggctttggg cgg
//
LOCUS       DBNGZA010000266         2673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_473400, whole genome shotgun sequence.
ACCESSION   DBNGZA010000266 DBNGZA010000000
VERSION     DBNGZA010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_473400"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="AC16FU_05595"
     CDS             complement(<1..1100)
                     /locus_tag="AC16FU_05595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2993880.1"
                     /translation="MNGQRRPLRWFLFILAVSILVKFFLIFRVPLTPQETYYWNYACH
                     LALSYFDHPPLGAWTIAFFRWLIGDSAFAVRIGALGYSTGLAIGLFLLGKRFLNDRLG
                     LTTFLSITLTPMFWLGGGVMTPDAPLLCFWVWAFYFFLQALQKKNLTSWVLFGFCTGA
                     SGLSKYTAVFIPVAVLFSITLSRNLLVIKSKGLWLSVLTAGAVFSPVLIWNAQHHWAS
                     FLFQTSRRAGELGSFSITPFLQFLGSQAGVISPLTLAAFLFAPVWALVSGWRNRRTDL
                     LILFSWSFPIIFFFALVATRYWVKVNWVTPGYLAAALAYSFFCGGRDPGGSSKARRRW
                     YGASVGLGFLFVLLLTVAVASPHIALGTKADSL"
     gene            complement(1097..>2673)
                     /locus_tag="AC16FU_05600"
     CDS             complement(1097..>2673)
                     /locus_tag="AC16FU_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR2993881.1"
                     /translation="LILLALWVAAPVLAKTNRSIRRIQIERRDIFADGDSLLWYERLA
                     NRLHVLTKAGVIRRELTFREGEVLHPWFLEESERNLRQLGFLGTVKITADTLNDSGCT
                     VRVRTQDQWSTELAASAQGGGGAYVLRLSGSEKNLFGWGKTIEAAYEKTDLRNQWNAS
                     VLDPRLFGSRLSAELAYEKTSEGKAVAGRLALPLFSIRSRYGFSLALSRQTDILRFFD
                     KGEEAIFYNFRMINFSFRAVRSFGDSLKFDPFFLTEYAERDFSGPFRFAGFAGDPASV
                     VANYVFPEPKATRLGLGLQVHRSRFMEGRFYDNFGNVEDIGLGPSGEISFAGSHQRLN
                     STRTRWSPALLLNHTRSFGSAHLVRLTFSVENDFLSDRWEGARTSARFIHYWRWGTRQ
                     TLAWQIRYVAITGVNRVGQLLLGGDNGLRGYDARRFSGNKRLLVNFEQRLFAPVEILT
                     VGLGAVAFVDFGSAWSPGTAPALSSLRSDAGVGLRLGLLKSYGFKVLRLDWAKALNEP
                     GSSFSFGTGMTFGLNL"
BASE COUNT          695 a          755 c          803 g          420 t
ORIGIN      
        1 agactgtcag cctttgtgcc taaagcgatg tggggtgagg cgaccgccac cgtcaaaagg
       61 aggacaaaaa gaaatcccaa accgactgag gctccatacc accttcgacg ggctttgctg
      121 ctgcctcccg gatccctccc cccacagaaa aaggaataag ccagcgcggc ggcaagatag
      181 ccgggcgtga cccagttcac tttgacccag tagcgcgtgg cgacgagcgc aaaaaagaag
      241 ataatcggaa acgaccagga aaaaagaatc aacaggtcag tccggcgatt gcgccaaccg
      301 gaaaccaagg cccagaccgg agcaaaaaga aaggccgcga gggttagagg tgaaatgacc
      361 ccggcctggg agcccaggaa ttgcaaaaat ggcgtgatgg aaaaactccc cagttccccg
      421 gcccggcgcg aggtttgaaa gaggaacgag gcccagtggt gctgggcgtt ccagatgagt
      481 accggagaaa aaactgcccc ggcggtaagc acactcaacc acagcccttt ggacttgatg
      541 accaaaagat ttcgcgagag cgttatcgag aataaaaccg caaccggaat gaagaccgca
      601 gtgtatttcg agagtccgga cgccccggta cagaagccga atagtaccca cgaggtaagg
      661 ttcttttttt gcagggcctg cagaaaaaaa tagaacgccc agacccagaa gcaaagcaag
      721 ggggcgtccg gagtcatcac cccgccgccc agccagaaca tgggggtcag ggtgatactg
      781 agaaaggttg tcagaccgag ccggtcattg aggaaacgct tccccagcaa aaataagcca
      841 atagccaatc cggtggagta tcccaaagca ccgatacgca cggcaaaagc gctgtcaccg
      901 ataagccagc ggaaaaaggc gatcgtccac gcgcccaaag ggggatgatc gaagtacgaa
      961 agcgcgaggt gacaggcgta gttccagtag taggtttcct gcggcgtgag cggcacacgg
     1021 aaaatcaaga agaatttcac caagatggaa acggctagga tgaagagaaa ccagcggagg
     1081 gggcgccttt gaccgttcat aaattcaaac caaaagtcat accggtgccg aaactgaaag
     1141 acgagcccgg ttcgttcaag gccttggccc agtcgagacg cagcacctta aaaccatacg
     1201 acttgagcaa ccccaggcgc aagcccactc ccgcgtcgga acgaaggctt gaaagcgctg
     1261 gggccgtccc cggagaccag gcggagccga aatcgacaaa cgccaccgcc cccaggccga
     1321 cggtcagaat ctctaccgga gcgaagaggc gctgctcgaa attaacgagg agacgcttgt
     1381 tgccggaaaa cctgcgggca tcgtagcccc gtaacccgtt gtcgccgcct aagagcagtt
     1441 gcccgactcg gttgacgccg gtgatggcaa cgtagcgaat ctgccaggcc agggtctgcc
     1501 tggtgcccca gcgccaatag tggataaagc gggccgaggt gcgcgcccct tcccagcggt
     1561 cagacaaaaa gtcgttctcg accgaaaagg tcagacgaac gaggtgcgct gagccaaacg
     1621 atcgggtgtg gttcaagagc aaagctgggg accagcgcgt gcgggtagaa ttcaggcgct
     1681 ggtgactgcc ggcaaatgag atttcgccgg acggaccgag cccaatgtct tccacgttcc
     1741 cgaagttgtc gtaaaaacgg ccttccatga agcggctgcg atggacttgt agacccagac
     1801 ccaggcgcgt cgctttcggt tcgggaaaga catagttggc gacgaccgac gccgggtcgc
     1861 ccgcaaaacc agcgaagcgg aatgggccgg agaaatcacg ttcggcgtac tcggtcagaa
     1921 aaaacggatc gaacttcaga ctgtctccaa aggagcggac ggcgcggaac gagaaattga
     1981 tcatgcgaaa gttatagaag atcgcttcct ctcccttatc aaaaaaacgg agaatgtcgg
     2041 tctggcggga gagggcgagg ctgaaaccgt agcgcgaacg gatggagaaa agggggagtg
     2101 ccaggcggcc ggccacggcc ttcccctcgg aggttttttc atacgccagt tccgcgctca
     2161 agcggcttcc gaaaaggcgc gggtcaagaa cggacgcatt ccactggttg cggaggtcag
     2221 ttttttcgta ggccgcttca atggtcttcc cccacccgaa caaattcttc tccgaaccgc
     2281 tcaagcggag gacgtaggcg cccccgccgc cctgcgccga agccgccagt tcggtggacc
     2341 actggtcctg ggtccgcacg cgcaccgtac agccggagtc gttcagcgta tcggcggtga
     2401 tcttcacggt gccgaggaag ccgagctggc gcagattccg ctcgctttct tcgaggaacc
     2461 acgggtggag cacttcgcct tcccggaagg tcagttcccg gcggatcacc cctgctttgg
     2521 tcaaaacgtg aagccggttg gccagacgct cgtaccagag aagactgtcg ccgtcggcga
     2581 aaatatcccg ccgttcgatc tggatgcggc ggatgctccg gttcgttttc gccaaaaccg
     2641 gggccgccac ccaaagggcc agaagaatca aaa
//
LOCUS       DBNGZA010000267         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1108468, whole genome shotgun sequence.
ACCESSION   DBNGZA010000267 DBNGZA010000000
VERSION     DBNGZA010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1108468"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="AC16FU_05605"
     CDS             complement(<1..718)
                     /locus_tag="AC16FU_05605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR2993882.1"
                     /translation="MNLPPSDQSKRLFAKTRSEKKAYVQKLFADITPEYDRCNRILSL
                     WRDMAWRRKAVASLPSNSVVLDIAAGTGDMAIALLERKNFTGQVVLSDFTLGMLEEAR
                     HKLKERKLERNAFYVVADAENLPFKAGAFDGAMMGFALRNLTDVPACLGETKRVLVNG
                     GRAAFLEISHPENKLFAKFFYFYFYRVLPFLAHPFTRHPYAYKYLPDSLKIFLTQKEV
                     AGLCRQVGFREATYQNLMGGS"
     gene            complement(699..1136)
                     /locus_tag="AC16FU_05610"
     CDS             complement(699..1136)
                     /locus_tag="AC16FU_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993883.1"
                     /translation="MSAVEPYGRWVLFLSVVSFFFSSCGKSMSPNEGQVIRAQLGNFE
                     RALDSRQDSTVALFFVPQKGGERTAAQGLLRQLYSQPEIARVRLTNRRIEVVKKEATV
                     LCTVEALDAAGQKVASIPDRRAMLRLVKQHGTWLVTSYELTAL"
     gene            complement(1178..1879)
                     /locus_tag="AC16FU_05615"
     CDS             complement(1178..1879)
                     /locus_tag="AC16FU_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993884.1"
                     /translation="MSGGFIVWGYEHFGAYTFTNELWESPLDYDKNGEVTEKEMLKWN
                     DEIMHGKAFVDWHPFKHPTLGEIEIGGWDKFHTRMPPTDYLEDLCLRNCLFTLYNAEM
                     MAQLKIKEVQTQPAAGAVKLTVTVTNEGFMDTNTGMADSVKISKPVIVRLEHGPEVEV
                     ISGGEKVKHTDRVRLATEGERKVVPERIDLGAVRGRSERLVEWTVVKKSSSKAQIKIT
                     VTSEKGGVDERVVSL"
BASE COUNT          410 a          576 c          537 g          403 t
ORIGIN      
        1 ccgagccgcc catgagattc tggtaggtgg cctcgcggaa acccacctgc cggcacaggc
       61 cggcgacctc tttctgcgtc agaaagattt tgagcgaatc cggcaggtac ttgtaggcgt
      121 acgggtgccg ggtgaagggg tgcgccagaa acggcaggac gcggtagaag tagaagtaaa
      181 aaaatttggc gaagagcttg ttctcgggat gggagatttc caaaaaagcg gcccgaccac
      241 cgttcaccaa cactcgtttg gtttccccca ggcaggctgg aacgtcggtc aaattccgca
      301 gggcaaagcc catcatggcg ccgtcgaacg ccccagcttt gaacggcaaa ttctccgcat
      361 cggcgacaac gtagaaggca ttgcgctcta acttgcgctc cttgagtttg tggcgcgctt
      421 cctcgagcat cccgagagtg aaatcggaaa gcacgacctg gccggtgaaa ttttttcgtt
      481 ccaggagcgc aatggccata tcacccgtgc cggcggcgat gtctaatacc acactattcg
      541 acggcaaact ggcgaccgcc ttccgccgcc aggccatatc gcgccagagc gagagaatcc
      601 ggttgcagcg gtcgtactcc ggcgtgatgt cggcgaacaa cttctgaacg taggcctttt
      661 tctcggagcg agtcttcgcg aagagtcgct tcgactggtc agagggcggt aagttcatag
      721 gaggtaacga gccaggtacc gtgctgtttt acgagacgga gcatcgcgcg tcggtcgggg
      781 atggaggcta ctttctgtcc agcggcatcc agcgcttcga ccgtgcagag gacggtggct
      841 tcctttttga ccacctcaat ccggcggttg gtcaaacgca cgcgggcaat ctccggctgg
      901 ctatagagtt gccggagcaa cccctgcgct gcagtgcgct cgcccccttt ctgcggcaca
      961 aagaagagtg cgacggtgga gtcctgccgg gaatcgagcg cccgctcgaa attccccaac
     1021 tgcgcccgga tgacctggcc ttcgttgggg ctcatcgatt tgccgcagga tgaaaagaag
     1081 aacgaaacaa cggagaggaa cagaacccaa cgcccatagg gttcgactgc gctcaccctg
     1141 aaaagggcta agctaccagg accaaccatc agacctgcta cagggagacg actctctcgt
     1201 caacgccgcc cttttccgag gttacggtga tcttgatctg cgctttgctg gaactcttct
     1261 tcaccaccgt ccattccacc agtctttcgg aacggccccg caccgcgccc aaatcaatac
     1321 gctccgggac cactttccgc tcaccttcgg tggccagacg aacccggtcg gtgtgcttca
     1381 ccttctcccc gccggaaatg acctccacct ccggcccgtg ctctaaccga acaatcaccg
     1441 gttttgaaat tttcaccgaa tccgccatcc cggtgttcgt gtccatgaat ccctcattgg
     1501 tgactgtgac ggtgagtttg accgcgcccg cagccggttg cgtctggacc tctttgattt
     1561 tcaactgcgc catcatctcc gcattgtaga gcgtaaagag gcagttccgc aggcagagat
     1621 cctccaggta gtcggtgggc ggcatccggg tgtggaattt atcccagccg ccgatttcaa
     1681 tttcgcccaa agtcggatgc ttgaacgggt gccaatcgac gaacgccttc ccgtgcataa
     1741 tttcgtcgtt ccacttgagc atttcctttt cggttacttc accgttctta tcgtaatcca
     1801 gaggggactc ccacaattca ttggtgaagg tataggcgcc gaagtgctcg tacccccaga
     1861 cgatgaagcc gccgctcacc gggtagaggt cgaggaaaac cagccaagcc tagtagccgg
     1921 gtaaaa
//
LOCUS       DBNGZA010000268         6228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1428220, whole genome shotgun sequence.
ACCESSION   DBNGZA010000268 DBNGZA010000000
VERSION     DBNGZA010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1428220"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="AC16FU_05620"
     CDS             complement(<1..1487)
                     /locus_tag="AC16FU_05620"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2993885.1"
                     /translation="MQPKRSHYRRLVRFLKPYWKHATVSMVSMTLFALLSGAMIWMIG
                     PLAGTLFGLGGKSVMTAQTPDSANQTGTPELLKPYSGLKAKLKAPFERFFHHPDKLTV
                     LERLCIAVLLIVVLKSLFFYLQGYLMAYVQQSVIRDLRNTLYRHYHTLSLSFFNKART
                     GQLISRITNDVAVVNEMLEMALTRLVRDPILVIIFIFILLVISWQLTLVAFVAFPLLM
                     LGVSQIGKAIHRYSQRSQERIADVNAAIQEAVSGDRVVTAFGAERYEVAKFEALTKAY
                     RKVMLKMGRVRILSSPINEILATAAGVVILWFGGKQVLSGHLLKPEDFLLYLFAMFSL
                     IAPAKSLSLAHAKVQEGMAAAARIFEIIDTAPHIVEDPQAQPVTGFRLKIVFADVSFR
                     YDTGPWVLRDINLSVNAGEVVAVVGPSGSGKSTLVDLLSRFYDPTEGSIALDGLDLRR
                     IKTGSLRTLIGIVTQETILFHDTVFNNIAYGLEGVTELRVKGAAEA"
     gene            complement(1496..2620)
                     /locus_tag="AC16FU_05625"
     CDS             complement(1496..2620)
                     /locus_tag="AC16FU_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2993886.1"
                     /translation="MKRFEKLIKQAALRLLRLIWGNSPIPGAFRREAVRSIVVVRQDE
                     RLGNMVLITPFLSGLRKAFPDASLTLVASEKYGHLFDDDNCIDRLVLVNKKRCLFNPV
                     YLLKTIQQLRNIRADLAFDLSDAEQISALNSIATRLTGARWRIGFDRPEAATFLNRLV
                     PVPEVPCHATEMHLRLLQALVPGSYDSAPAIRVDAGAREWAKEWLQRKRQPLESRLVA
                     VHIGARGPKQWGVENFFELIESLTADGNVKVVVITGPQEHHLLEQKKKDDRNVLLAPK
                     MSVPQLAALLSLCDLYVGNDAGPLHLAVAVGTPTVALFFTSDALKYGPRGKKHIILAT
                     LGRRPSVAEVYRSIHLALTGIDRPGGRSRSTQVAVAAGIP"
     gene            complement(2627..3424)
                     /locus_tag="AC16FU_05630"
     CDS             complement(2627..3424)
                     /locus_tag="AC16FU_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993887.1"
                     /translation="MSGHLKLLWTATGVVLLMVFTGCAGPRSISRVDPVAVLSQAKQE
                     RTSTAFTTASFRVKFSLSTQAGSGAGIAYLYAKKPDTLAIEVPGVFGAGAFRARIDGA
                     SHLFIYIPEEKAYYDGLLAQFPAEGIFWTPTVWQSPAWDVFFGNSSPLGGDDDTLTFV
                     GERKGLYFYGEDDQGWQHEIGINPRDGKARQELWITEDSAVIWRIRYGRSRQGKTGPP
                     IDRIDLQTEWGEKFSLQFQEANFDVPISPRKFSTDVPAGARRIEPLR"
     gene            complement(3434..4081)
                     /locus_tag="AC16FU_05635"
     CDS             complement(3434..4081)
                     /locus_tag="AC16FU_05635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR2993888.1"
                     /translation="MERLSEFVLLTPSILFALTFHEFAHAWMANRLGDPTARDLGRLT
                     LNPLAHLDFFGTLMIFLSDFRFGWAKPVPFNPYNLKEPKRGILLIALAGPVANMIIAL
                     ACGLIIRFSSGAYDPHNTTITGIIFKMVVQSLLLNLSLAFFNLIPVPPLDGSKIMYGL
                     LPDRYAYQFAMWERVGPLVLIGLILLGSFSGFNILWFIFSPFVRLFAFLFAGISF"
     gene            complement(4144..5436)
                     /locus_tag="AC16FU_05640"
     CDS             complement(4144..5436)
                     /locus_tag="AC16FU_05640"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR2993889.1"
                     /translation="MTTLIETSPPELKSEYPHSSTLDNAMRQVDMAAQKLGMDPGFLE
                     IIKLPRRAVILNLPIQMDDKTFRVFTGYRVQHSIVRGPGKGGIRYHPDVTLDEVQALA
                     AWMTWKCAIANIPFGGAKGGIAVDPEKLSKGELERLTRRYIAELIEVIGPDKDVPAPD
                     VNTNSQTMAWVMDTYSMHARHTVTSVVTGKPVELGGSLGRREATGRGVMLTIREAAKH
                     LGMLLPGATVAVQGFGNVGSIAALELHKLGCKVIAASDVHGGIFNPKGLDIPQLIDHV
                     TRRKSVVDFPGAAAVSNEDLLTTRCDILVPSALENQITGENAEGIKAKIIGEGANGPT
                     TPEADTILDRKGVFVIPDVLCNAGGVTVSYFEWAQDRSGYFWSEEEVNTRLERILVKA
                     FADVLAMSLANKVNMRVAAFMVALRRVADVMHLRGMYA"
     gene            complement(5420..6118)
                     /locus_tag="AC16FU_05645"
     CDS             complement(5420..6118)
                     /locus_tag="AC16FU_05645"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR2993890.1"
                     /translation="MLPKSVGTSEIERPVKEKLTRDFYLLPTLEVARNLLGTYLVVNH
                     RRKQLVGRIVETEAYLGLEDPASHAYRGQTERNRVMWDEGGLVYVYFTYGMHHCLNVV
                     TEAPGKAAAVLIRAVEPVRGVEEMEKRRRMRLGKLTALSKVEGRLGPDLTNGPAKLCS
                     AFGIDKSFYGADLCGDHIWIEKRERPWEPIGRSGRVGVNAASDQPWRFYLFGNRFVSK
                     ARPAREENNHDHAH"
BASE COUNT         1381 a         1772 c         1807 g         1268 t
ORIGIN      
        1 gcttccgccg cgcctttcac ccgaagttcg gtgaccccct ccagaccgta ggcgatgttg
       61 ttgaacaccg tgtcgtggaa gagaatcgtc tcctgcgtga cgatcccgat cagggtccgc
      121 agacttccag ttttgatgcg ccgcaaatcg agaccatcga gagcgatgct cccctcggtg
      181 gggtcgtaaa aacgcgagag gaggtctacc agggtcgatt tgcccgagcc ggagggcccc
      241 acgacggcga ctacctcacc ggcgttgact gagagattaa tgtcgcgcaa cacccagggt
      301 cccgtgtcgt agcggaaaga gacgtcggcg aaaacgatct ttagtcggaa cccggtgacg
      361 ggctgggcct gcgggtcttc gacgatatgc ggggcagtgt ctataatttc aaaaatgcgc
      421 gccgcggcgg ccatcccctc ctgtaccttc gcatgcgcca ggctcaaact cttggccggt
      481 gcaatgaggg aaaacatcgc aaaaagatac aaaagaaaat cttccggctt gagcaagtgg
      541 ccggagagca cctgttttcc tccaaaccag agaatgacca ctcccgcggc ggtggctaaa
      601 atttcattta tcgggctgga gaggatgcgt acccggccca ttttgagcat caccttccgg
      661 taggccttgg tcaacgcttc aaacttggcc acctcgtagc gttcagcgcc gaaggccgtc
      721 accacccggt caccggagac cgcttcctgg atcgccgcgt tcacgtcggc gatccgctcc
      781 tgcgagcgct ggctgtagcg gtgaatcgcc ttgccgatct gggagacccc cagcatcaag
      841 aggggaaagg caacgaacgc cacgagcgtc aactgccagg aaatcaccag gaggatgaag
      901 ataaaaataa tgactaagat cggatcgcgg accaggcgcg tgagcgccat ctcgagcatc
      961 tcgttcacca ctgccacgtc gttggtgata cgggagatca actggccggt gcgggccttg
     1021 ttgaagaagg aaagcgagag cgtatggtag tgccggtaga gcgtattgcg caggtcgcgg
     1081 atgacgctct gctgtacata ggccatcagg tagccctgca ggtagaagaa gaggcttttc
     1141 aacaccacga tgagcaaaac ggcgatgcag aggcgctcaa ggaccgtcag tttgtccggg
     1201 tgatggaaaa accgctcgaa tggcgctttg agtttggcct tgagaccgga atagggcttc
     1261 aggagttcgg gcgtgccagt ctggttcgca ctgtccgggg tctgggcggt catgacgctt
     1321 tttcccccca gaccgaagag cgttcccgcc agagggccga tcatccagat catcgccccg
     1381 ctcaaaagcg caaagagggt catgctcacc atcgacaccg tggcatgctt ccagtacggt
     1441 ttcaggaagc gaaccagtcg ccggtagtgg gagcgtttcg gctgcatggg agtgctcacg
     1501 gtatccctgc ggcgacggct acttgagtcg atcggcttct tccacccgga cgatcgattc
     1561 ctgtcagcgc taaatgaatt gatcgataga cttcggcaac cgacggcctc cgccctagag
     1621 tggcgagaat aatatgtttt ttcccccgcg gcccgtactt cagtgcatcc gaagtgaaaa
     1681 ataacgcaac cgtcggcgtt cccaccgcca cagccagatg gaggggaccg gcgtcgttgc
     1741 ctacgtacag gtcgcagagc gacagcaaag ccgccagttg gggtactgac attttgggag
     1801 caagcaggac gtttcgatca tctttctttt tctgctccag aagatgatgc tcttgtggcc
     1861 cggtgataac gaccactttc acgttgccgt cggcggtcaa gctttctatg agttcaaaga
     1921 aattctccac accccactgt ttcgggccgc gggcgccgat atgaacagcg actaaacgcg
     1981 actccagcgg ctgtcgcttc ctttgcagcc actcttttgc ccactcacga gcaccggcat
     2041 cgacccgaat tgccggcgcg gaatcatacg agccgggaac cagtgcttgc aacagccgca
     2101 ggtgcatctc ggttgcgtga cagggaactt cgggcaccgg taccaaccga ttgaggaatg
     2161 tggcggcctc tggccggtca aagccgatcc gccagcgggc gccggtaagg cgggtcgcta
     2221 tgctattgag cgctgagatt tgctcggcgt cgcttaaatc aaaagcgagg tcagctcgga
     2281 tattgcgaag ctgttgaata gttttcagga gatagaccgg attgaataga cagcgtttct
     2341 tattcaccag aacgagccga tcaatgcaat tatcgtcgtc aaaaagatga ccgtacttct
     2401 cactggccac cagggtaaga gaagcgtcgg gaaacgcctt acgcaaaccc gaaaggaaag
     2461 gcgtaatgag caccatattc cccaggcgtt catcctgccg caccactaca attgaacgca
     2521 ccgcttccct gcggaacgct ccgggaatgg gtgaatttcc ccagataagg cgcaaaagtc
     2581 ttagtgccgc ctgcttaatg agtttttcga atcttttcat acctgttcag cgaagcggtt
     2641 caattcgcct ggcccccgcg ggaacgtcgg tactgaattt cctcggcgaa atcggcacgt
     2701 cgaaattggc ttcctgaaat tggagggaaa acttttcacc ccactctgtt tgtaaatcga
     2761 tccggtcaat gggcggtcct gtttttcctt gacggctgcg tccgtagcgg atgcgccaga
     2821 tcaccgcgct gtcctccgtg atccagagtt cctgacgggc cttcccgtcg cggggattga
     2881 tcccgatttc atgttgccat ccttgatcat cctctccata aaagtacagc ccttttctct
     2941 ccccgacaaa tgtcaacgtg tcatcatctc cgcccaaggg ggacgaattg ccgaagaaca
     3001 cgtcccaggc cgggctctgc cagaccgtcg gcgtccagaa aattccctcg gcggggaatt
     3061 gcgccagcag gccgtcgtag tacgcttttt cttccgggat gtaaatgaaa agatgagacg
     3121 ccccgtcaat ccgcgcgcgg aatgctcccg cgccgaaaac gcccggcact tcgatggcga
     3181 gcgtatcggg ctttttggca tacaggtagg cgattcccgc acccgaaccg gcttgggttg
     3241 aaagcgaaaa tttcacccgg aaactggcgg tggtgaaagc agtggaggtt cgttcctgct
     3301 tcgcctgcga taggacggca accgggtcaa cgcggctgat ggagcgcggc ccggcgcagc
     3361 cggtgaaaac catcaataag acgacacctg ttgccgtcca gagcagtttg agatggccgg
     3421 acatcctgcg tctttaaaat gaaatcccgg caaaaaggaa ggcgaacagg cggacgaagg
     3481 gagagaagat gaaccacaga atattgaacc cgctgaagct tccgagaagg ataagcccga
     3541 tgagcaccag aggccccacc cgttcccaca tggcgaactg gtaggcatag cggtcgggca
     3601 atagcccgta cataattttt gagccatcga ggggcggcac cggaatcagg ttgaaaaaag
     3661 cgagggacag attgagcagg agactctgca ccaccatttt aaagattatg cctgtgatcg
     3721 tagtattgtg cgggtcgtac gctccagagg aaaaacgaat gatcagcccg caggccaggg
     3781 caataatcat attcgccacc ggcccggcca aagcaatcaa gagaatcccg cgcttcggct
     3841 ctttcagatt gtatggatta aatggcaccg gcttggccca gccgaagcga aagtcggaaa
     3901 gaaaaatcat gagcgtcccg aagaagtcga ggtgcgccag aggatttaag gtcaggcggc
     3961 caagatcgcg ggcggtgggg tcgcctaacc ggttcgccat ccaggcgtgg gcgaactcgt
     4021 ggaaggtgag cgcgaagaga atcgaggggg ttaaaagaac gaattcagag aggcgttcca
     4081 ttcatccctc aaaaagcgaa agtcggggac atcccccgac ttgcatctaa agacaaatcg
     4141 aacttaagcg tacatacccc gcaggtgcat tacgtcggcg acgcgcctga gggccaccat
     4201 aaaggccgcc acgcgcatat tgaccttatt cgccagggac attgccagaa cgtcggcaaa
     4261 ggccttgact aaaatccgct ctaaccgggt gttcacctct tcctcgctcc agaaatagcc
     4321 cgagcggtcc tgcgcccact cgaagtagga aacggtcacg ccgccggcgt tgcagaggac
     4381 gtcggggatg acgaagacgc ccttgcggtc gagaatcgta tcggcttcgg gcgtggtggg
     4441 gccattggcg ccttccccga tgatcttggc cttgatccct tccgcgtttt ctccggtgat
     4501 ttgattttcc agcgccgagg gcaccagaat gtcgcagcgg gtggtcaaaa ggtcttcatt
     4561 ggagactgcc gcggcgccgg ggaagtccac caccgatttg cggcgggtaa cgtggtcgat
     4621 caattgcgga atgtccagcc ccttcggatt gaagatgcca ccgtgcacgt cggaggccgc
     4681 gatcacttta catcccaact tgtggagttc caaagccgca atcgagccga cgttgccgaa
     4741 gccctgcacc gccacggtgg ccccgggcaa aagcatgccg aggtgtttgg ccgcttcccg
     4801 gatggtgagc attaccccgc ggccggtggc ttcccgccgg cccaaagaac cgcccagttc
     4861 caccggcttg ccggtgacga ccgaggtgac tgtgtggcgg gcgtgcatgc tgtaggtgtc
     4921 catcacccag gccatggtct gggaattggt gttcacgtcc ggcgccggca cgtccttgtc
     4981 cgggccgatg acctcgatca attcggcgat gtagcgccgg gtgagccgct ccaattctcc
     5041 tttggaaagt ttttcgggat cgacggcaat gccgcccttg gcgccgccga agggaatgtt
     5101 ggcgatggcg cacttccagg tcatccaggc ggctaaggcc tgcacttcat ccagtgtgac
     5161 gtcagggtgg tagcgaatgc cgcctttgcc cggcccgcgc acaatggagt gctgcacccg
     5221 gtagccggtg aaaacgcgga acgtcttgtc atccatctga atcgggaggt tgagaattac
     5281 cgcacggcgg ggaagtttga tgatttccag aaaaccgggg tccatgccta acttttgtgc
     5341 agccatatcc acctgccgca tggcattatc taacgtcgag gagtgcggat attcgctttt
     5401 gagttccggt gggctggttt caatgagcgt ggtcatggtt attctcctcc cgcgcagggc
     5461 gggcttttga gacaaatcta ttgccgaaaa gatagaagcg ccacggttga tcagaggcgg
     5521 cgttgacacc gacccggccc gaacgtccaa tcggctccca cggccgttcc cgcttttcaa
     5581 tccagatatg gtcgccgcac aaatcggctc cgtaaaaact tttgtcgatg ccgaacgcag
     5641 agcagagttt ggccgggcca ttggtgagat caggccccaa ccgcccttcg actttgctca
     5701 aggcggtgag cttgcccaac cgcattctcc gccgcttttc catctcttcc accccccgta
     5761 cgggttccac ggcgcgaatc aaaaccgccg cagctttccc cggcgcttcc gtcactacgt
     5821 ttaaacagtg gtgcatgccg taggtaaagt agacgtagac cagaccacct tcgtcccaca
     5881 tcacccggtt gcgctcagtc tggccccgat aagcatgact ggccgggtct tcgaggccga
     5941 ggtaggcctc ggtttcgacg atgcgcccga ctaactgctt tctgcggtga ttgacgacga
     6001 ggtaagtccc cagaagattc cgggcaacct caagcgtggg caaaaggtaa aaatcccgcg
     6061 tgagcttttc cttgaccggt ctttcgatct ctgatgttcc aacgcttttc ggtagcatgg
     6121 atgacactga ataatacgaa ttagaaatcg tgagaacaag agggattctc cgagggagaa
     6181 atggtacctg tgaatcgcgg gggcgaccga gggcggtcgc cctacgcg
//
LOCUS       DBNGZA010000269         8038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_896587, whole genome shotgun sequence.
ACCESSION   DBNGZA010000269 DBNGZA010000000
VERSION     DBNGZA010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_896587"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16FU_05650"
     CDS             complement(<1..304)
                     /locus_tag="AC16FU_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR2993891.1"
                     /translation="MAAKRIYEIAKEYKVSSEAMMAIIKKLNIPVKSHMATASEELLA
                     AIALHFEKEKDRVKQDYAAKRQKALERQKVVPPPPPPVPLTVPEPAKAVPGAAPAKT"
     gene            complement(307..1659)
                     /gene="nusA"
                     /locus_tag="AC16FU_05655"
     CDS             complement(307..1659)
                     /gene="nusA"
                     /locus_tag="AC16FU_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR2993892.1"
                     /translation="MSADIMDALQQIAHEKNIELDYVVETLEVGLLTAAKKKYGNADN
                     ITVKLNRRSGQIDVVAKKKVVAAVADAALEIAVADARALNPDVNEGEEIDIPLPLEEF
                     GRNAIAAAKQTLIQRVREAEREKIYNEFIQRVGEVVSGHVQQIERGNLIINLVKTEAY
                     LPLKEQIPRERYRQGDRIRALITDVQKTARGPQIMLSRIHPDLLVRLFELEVPEIFER
                     IIEIKAVAREPGERSKIAVSSADERIDPVGACVGMKGSRVQAIVRELNNERIDIIPYH
                     SDPELFVSRALSPAKTLSLQAATDEHRMDIVVADDQLSLAIGKNGQNARLAAKLTGWK
                     VNITSESEFNARKKARREEKIDLRKLAGIGDKVGKKLMEQGIDSVQTLAAASVEQLVE
                     VEGIGEKKATKLIAQAQTYLQEAGKVPPPGEEEGTALAEEKAVAETGGPDEKEQATEE
                     "
     gene            complement(1697..2143)
                     /locus_tag="AC16FU_05660"
     CDS             complement(1697..2143)
                     /locus_tag="AC16FU_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR2993893.1"
                     /translation="MEGIRERLVEIIAPVVEREGYELVDLKVGRQGARALVRVFADRR
                     GGITVGQCADLSRAIADRLDLDDPLAEGYVLEVSSPGLDRPLETAADFRRKVGERVRL
                     TIAAADRPIILEGALTKSSDEVAELLVDGEPVVVELARITRAQVIV"
     gene            complement(2288..4021)
                     /locus_tag="AC16FU_05665"
     CDS             complement(2288..4021)
                     /locus_tag="AC16FU_05665"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR2993894.1"
                     /translation="MRWSELYIPTLRDDPADAELVSHKLLVRAGYVRKLTSGVYVYLP
                     LMQRVLAKISQIVREEMNKKGAQEITMPVLHPAELWQASGRWESIGKELFRLKDRHQR
                     EMALGPTHEEIVTNLVKGEIRSYRQLPLNLYQIQVKFRDEIRPRFGLMRCREFPMKDA
                     YTFDASEESFKQSYQKMLDAYRSAFLRCGLETKMVESDTGAMGGKAAHEFVVIVETDG
                     GESEILFCQKCDYAAHIDKAMSVEPNPPAADGKPKPNQKVDTPGQKTVEEVSRFLGVT
                     PRDLVKTLLYRADGKIVAALIRGDRQINEVKLKNALGVLELEMADAATVEKVSRAPIG
                     FAGPVGLSGATFVCDPEVTKLVNFVTGANEADRHLINVNINRDFAPDRVADIRNAAPG
                     EFCPRCQQGVLQSARGIEVGNTFQLGTKYSESLGATFIDEHGQEKPFIMGSYGIGITR
                     TTQAAIEKFHDQAGIIWPLSIAPFAVEILPVNPGDEKQKSLALSLYEELTKQGIEVLL
                     DDREERAGVKFKDADLIGIPLRITIGEKSLAENMVEIKIRKTGETIKPAVPQAVTKTN
                     EVLSRLAPDLG"
     gene            4219..4290
                     /locus_tag="AC16FU_05670"
     tRNA            4219..4290
                     /locus_tag="AC16FU_05670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4252..4254,aa:Arg,seq:ccg)
     gene            complement(4381..6960)
                     /locus_tag="AC16FU_05675"
     CDS             complement(4381..6960)
                     /locus_tag="AC16FU_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2993895.1"
                     /translation="MKRIQLASKFLSWTIFFVVTAAFPASADRYKPFGDELSTRHWPP
                     DRSYDLQHIRLDLTFDWDQKKVSGKVTYRLVPLNSGFREVRLNQGDLNITGVTDAAQK
                     SLVYAADDSFLVVKLPEAYDAGKPIEFSVAYWAKPRKGLYFVGPDSGYPTKPKQIWSQ
                     GEDEDNHFWFPCWDFPNDRATSEMVATVPETWTVVSNGRLVEVKPNPAQKTKTFHYSQ
                     EQPHVTYLISVVAGEFEKYADDFDGTPVEYYVPLGTGEAKARRSFGITPDAVKFFSEK
                     IGFRYPYAKYAQSAVTDFIYGGMENISATTQTDRTLHAEVSEPVVSSEGLVAHELAHQ
                     WWGDLVTCNEWAHAWLNEGFATYFASLYTEHHRGKDEFQYEMLNHARAYISEDENEYR
                     RPIVDNWYTDSDDLFDSHLYPKGGWVLHMLRGMLGDNLFWKGINNYAKTYAYQTVTTR
                     EFQKVMEEATGRSLGWFFDQWVYKGGYPRFEVHADWDEEHKVEVVKVKQTQTPDTLTP
                     VFRAPLIIELVDSSGTRSNPVEITQADQQFTFPASRRPLEVRFDPGNTILKTLDFPKE
                     KKELFYQVRFDPDVVARIWACGELGKKFADPEVVDTLAAVLTGDSFYGVRGAAASALG
                     KLQTGAAEKTLYVGYNDKDARVRRAAVDALGEFKESQTATDFLKKVIAGDPNDYVKAS
                     AVSSIGRIAAPGAFEILKQALRQPSEYDVVRTAAAGAFGTLKDKKAIDLLMPETRYGK
                     TPSVRTAAIGALGKVGKEEDSVTRRLLLLLNDPWLWVRGSAMDALAELKIAEAIPAIE
                     GHAQNEPDGRLRKQARNAIKRIREGKAETEDMAALRSELERLKSEQKLLRDRIEVLEK
                     HAP"
     gene            complement(6957..7442)
                     /locus_tag="AC16FU_05680"
     CDS             complement(6957..7442)
                     /locus_tag="AC16FU_05680"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2993896.1"
                     /translation="MAVYIISDTHIPDRVKAIPKKFLERLTPEDIVLHAGDFTTPVVL
                     EELRRRAKVHAVWGNMDGPEVRKELPRKTVVTLGKVKIGLYHGHGAPEGLVDRVRAEF
                     TEPVDIIVFGHSHTPFSKKFGATLLFNPGSLSINFDRRTLTYGRLQTDPELHIELVEI
                     P"
     gene            complement(7616..7900)
                     /locus_tag="AC16FU_05685"
     CDS             complement(7616..7900)
                     /locus_tag="AC16FU_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2993897.1"
                     /translation="MTKADLVEQVAGKTGLTRTDVAVVVDSFLEAVKTALEGGSNIEI
                     RGFGTFKVKVRKARKARNPRTGDEVPVPDRKVPVFKPSNEFKGLIIKQPV"
BASE COUNT         1482 a         2385 c         2246 g         1925 t
ORIGIN      
        1 gtgtttttgc gggcgccgct ccggggacgg ccttggcggg ctctggtacg gtcagcggca
       61 cagggggggg tggtggcggc accactttct gccgctccag cgctttctgc cgcttggcgg
      121 cataatcctg tttcacccgg tctttctctt tctcaaagtg gagcgcaatg gcggccagaa
      181 gttcttccga ggcggtagcc atgtggcttt tgaccgggat attcagtttt ttgataatcg
      241 ccatcatggc ctcgctggag accttgtact ctttggcgat ttcgtaaatc cgtttggccg
      301 ccatggttac tcttcggttg cctgttcttt ctcgtccggt ccgccggtct ccgccaccgc
      361 tttctcctcc gcaagagcag tcccttcttc ttctcccggc ggaggaactt tgccggcttc
      421 ctggaggtag gtctgcgctt gcgcaatcag tttagtagct tttttctcgc cgatgccttc
      481 aacttcaact aattgttcca cgctggcagc cgccaacgtc tgaaccgaat caatgccctg
      541 ctccatcagc tttttgccga ctttgtcgcc gatgccggcc agtttccgca ggtcaatttt
      601 ctcctcgcgg cgggccttct tgcgcgcgtt gaactccgat tcgctggtga tgttgacctt
      661 ccagccggtg agcttggccg ccaggcgggc attctgtccg ttcttgccga tggccaaaga
      721 gagctgatcg tcggccacca cgatgtccat gcggtgctca tcggtggcgg cctggaggga
      781 gagcgtcttg gccggcgaga gggcgcgcga gacgaagagt tccggatcgg agtggtaggg
      841 gatgatgtcg atccgttcgt tgttcaactc ccggacgatc gcctgcaccc gggacccttt
      901 catgccgacg caggcgccca ccggatcgat ccgttcgtcg gccgaagaga cggcgatttt
      961 ggagcgttcc ccgggttccc gagccaccgc tttaatctcg ataatgcgct cgaagatttc
     1021 cggcacttcc agttcgaaca gccgtaccag gagatcgggg tgtatccggg agagcatgat
     1081 ctgggggccg cgggccgtct tctgcacgtc ggtaatgagc gcccggatcc ggtcgccctg
     1141 acggtagcgc tcccgcggaa tctgttcttt gagcggcagg taggcttcgg tcttgaccag
     1201 gttgataatc aaattgcccc gctcaatttg ctggacgtgg ccggagacca cttcccccac
     1261 ccgctggatg aactcgttat agattttttc gcgctccgct tcccgcaccc tttggattaa
     1321 ggtctgcttg gccgccgcaa tggcgttgcg cccgaattct tccaggggga ggggaatgtc
     1381 gatttcctct ccctcgttca cgtccgggtt caacgctcgg gcgtcggcta cggcaatttc
     1441 cagagcggcg tcggcgaccg ccgccacgac tttcttcttg gcgactacgt cgatctgtcc
     1501 cgaacgccgg ttcagtttga ccgtaatgtt gtccgcgttg ccgtacttct ttttggccgc
     1561 ggtcaaaagc cctacttcca gggtctcgac gacgtagtcc agttcaatat ttttttcgtg
     1621 cgcaatctgc tgcagcgcat ccatgatatc cgcgctcatc ggccttcctt ttgcaacaat
     1681 gttccgcctc tccggttcac acgatcacct gcgccctagt gatgcgggcc agttcgacta
     1741 caaccggctc cccgtccacc aggagttcgg ccacctcgtc gctgctcttc gtcaacgccc
     1801 cttccaggat tatcggccgg tccgcagcag cgatggtcag ccgcacccgc tcgcccactt
     1861 tgcggcggaa gtcggcggcg gtctccagcg gacgatccag tccgggcgaa gaaacttcca
     1921 gaacgtaacc ttcggcaagg gggtcatcta agtccagccg gtcggcaatc gcccgggaga
     1981 ggtcggcgca ctggcccacc gtgatgccgc ctcgccggtc ggcaaaaacc cggaccaggg
     2041 cgcgggcgcc ctggcggccg actttcagat cgaccagttc gtacccttca cgttccacaa
     2101 ccggagcgat aatttccaca agtctttcgc gtatcccttc cattttccca cacttgccgg
     2161 aacatgcctc acggtcaggt tcatttgccc cgagggtcgt ggcccggctg taaacacaat
     2221 accaaaaagt gggccaccgc ccacttccag accgaacaca ctaatttacc accccaagaa
     2281 aaaaaaatca acccaaatcc ggggcgagcc gggacaaaac ctcgttcgtc tttgtaaccg
     2341 cttgaggtac agcgggtttt atagtctctc ccgttttgcg gattttgatt tccaccatat
     2401 tttcggccag ggatttttcg ccaatagtga ttcgcagtgg aatgccgatc aaatcggcgt
     2461 ctttgaattt gacgccggcc cgctcctccc gatcgtccag cagcacctca attccctgtt
     2521 tggtcaattc ctcatagagc gacagcgcca gcgatttctg cttttcgtcg ccggggttca
     2581 ccggcaaaat ctcaacggcg aagggcgcaa tcgagagcgg ccagataatc ccggcctggt
     2641 cgtgaaactt ttcaatggcc gcctgagtgg tgcgggtgat gccgatcccg taggagccca
     2701 tgatgaaggg cttttcctgc ccgtgctcat cgatgaaggt cgcaccgagc gattcgctgt
     2761 acttggttcc caactgaaag gtgttgccga cttcaatacc ccgcgccgac tgcaggacgc
     2821 cttgctgaca gcgggggcag aattcaccgg gtgccgcgtt gcggatgtcg gccacccgat
     2881 cgggtgcaaa gtcgcggttg atgttgacgt tgatcaagtg ccggtcggct tcgttagcgc
     2941 cggtcacgaa gttaaccagt ttcgtcactt ccggatcgca gacaaaagtt gccccggaaa
     3001 gtcccaccgg cccggcaaaa ccgatcggcg ctctggagac tttttcaacc gtggccgcat
     3061 cggccatttc cagttcaagg actcccaggg cattttttaa tttcacttcg ttaatctgcc
     3121 ggtcgccgcg gatcaaagcc gccacaattt tgccgtcggc ccggtagaga agagttttca
     3181 ctaagtcgcg cggcgtcacg cccagaaagc ggctcacctc ttccacggtc ttttgtccgg
     3241 gggtatcgac tttctggttt ggtttgggtt ttccgtccgc tgccggtgga tttggctcga
     3301 ccgacatcgc tttgtcgatg tgggcggcgt agtcgcattt ttggcagaac aaaatttcgc
     3361 tctcgccgcc gtcggtttcg acgatcacca cgaattcgtg ggccgccttc ccccccatcg
     3421 cgccggtgtc ggattcgacc attttcgttt ccagcccgca gcgcagaaag gcgctccggt
     3481 aggcgtcgag cattttctgg taactctgtt tgaaactttc ctcgctggcg tcgaacgtgt
     3541 aggcgtcttt catcgggaac tcccggcagc gcatcaaacc gaagcggggg cggatctcgt
     3601 cccgaaattt cacctgaatc tggtacaggt tcaagggcaa ttgccggtag gagcggattt
     3661 cacctttgac caggttggtg acgatttctt catgcgtcgg gcccaaggcc atttcgcgct
     3721 ggtggcggtc tttcagccgg aacaattcct tgccgatgga ctcccacctg ccggaggcct
     3781 gccagagttc cgccgggtgc agcaccggca tcgtgatctc ctgcgcccct ttcttattca
     3841 tctcctcccg cacgatctga gaaattttgg ccaggacgcg ctgcatcaaa ggcaggtaga
     3901 cgtacacccc cgaggtcagt ttgcggacgt acccggcccg gactaacagc ttatggctga
     3961 ccagttcggc atcggccggg tcgtctcgca aggtggggat gtacagttcg ctccagcgca
     4021 tggtttgtaa ctcgtaattg gtaactaagt gacgctaagc gtaattatat gtgtgcagca
     4081 ggtgaagtca aactcgcttg aaatgccatt gacttcattc cccctccgga ctaaattctt
     4141 ctcaagtata ttttggagat acgatgagcg cttttctttc catcactaat cacttgtcac
     4201 tcatcactgc ttttattcgc ccccgtcgcg taatggacaa cgcaccagcc tccggagctg
     4261 gccatgtagg ttcgattcct accgggggta tgtaactgca gggatcagag atcagggatt
     4321 agggatcggt aaaatctttt aactccaaca cctcaacaca tcgatacttc gacacttttt
     4381 ttagggcgcg tgcttctcta agacctcaat ccggtcgcgc aaaagtttct gctcagattt
     4441 caaacgttcc agctcgctgc gcagggcggc catgtcttcg gtctcggctt ttccttctct
     4501 tatcctttta atggcattcc gcgcctgctt gcgcaggcgg ccgtcgggtt cgttctgtgc
     4561 gtgaccttcg attgccggaa ttgcctccgc aatcttcaat tcggctaaag catccatcgc
     4621 tgacccacgc acccacagcc aggggtcgtt caaaagcaag agaagccgcc gggtgacgct
     4681 atcctcctcc ttccctactt tccccaacgc gccaatggcc gccgtgcgga ctgaaggcgt
     4741 tttgccgtag cgcgtctcgg gcataagcag gtcgatggcc ttcttgtctt tcagcgttcc
     4801 aaaggcaccg gcagctgccg tgcgcaccac gtcgtactct gagggttggc gcagggcttg
     4861 cttcaaaatc tcaaacgcac ccggcgcggc aatccgacca attgaggaga cagcgctggc
     4921 cttcacgtag tcgttgggat ccccggcgat aacttttttc aagaagtcgg tggcggtctg
     4981 gctctctttg aattccccca acgcatcgac cgccgcccgg cggacgcggg cgtctttgtc
     5041 gttataccca acgtagaggg ttttctccgc cgcgcccgtc tgaagttttc ccaaggccga
     5101 agccgctgcg cctcgcacgc cgtagaatga atctccggtc aaaacagccg cgagcgtatc
     5161 gaccacttcc ggatcggcaa acttctttcc caactccccg caggcccaga tgcgggctac
     5221 cacgtccggg tcaaagcgca cctggtaaaa gagttctttt ttttctttgg gaaagtccag
     5281 agttttgagg atggtattcc ccggatcaaa gcgtacttcc agcggccgtc gcgaagctgg
     5341 gaaggtgaac tgctgatcgg cctgagtgat ctccacggga ttcgagcgcg ttccgctgct
     5401 gtccacgagt tcgattatca gcggtgcgcg gaaaactggc gtcaacgtgt cgggcgtctg
     5461 cgtctgcttg accttgacga cttcgacttt gtgctcctca tcccagtcgg cgtggacttc
     5521 gaacctggga tagccgcctt tataaaccca ctggtcgaag aaccaaccca aagagcggcc
     5581 ggtggcctcc tccattacct tctggaattc ccgtgtggtg actgtctgat aggcgtaggt
     5641 tttggcgtag ttatttattc ccttccagaa gaggttgtcg cccagcatcc cccgcagcat
     5701 atgcaagacc cagccgcctt tgggatagag gtgggaatca aacaggtcat cggagtcggt
     5761 gtaccagtta tcaacaatcg ggcgtctgta ctcattttcg tcctcactga tataggcccg
     5821 ggcgtggttc agcatttcgt actggaattc gtccttgccc cgatgatgct cagtgtagag
     5881 cgaggcgaaa taggtcgcaa agccctcatt gagccaggcg tgggcccatt cgttgcaggt
     5941 gaccagatcg ccccaccact ggtgggccag ctcgtgcgcc accagtccct cgctggaaac
     6001 gaccggttca gaaacctcgg cgtgcaaagt ccggtcggtc tgggtggtgg cggagatgtt
     6061 ctccatcccg ccataaatga aatcggtcac ggcggactgg gcgtactttg catacgggta
     6121 gcgaaatccg attttttcgg agaagaattt cacggcgtcc ggagtgatgc cgaaggagcg
     6181 gcgggctttc gcttcccccg tgccgagcgg gacgtagtac tccacgggtg tgccgtcgaa
     6241 atcatcggca tacttctcaa attctcccgc gacgacggag atgagatagg taacgtgcgg
     6301 ctgctcctgt gagtaatgga aggtcttcgt cttttgcgcc gggttgggtt tgacctcgac
     6361 cagacggccg tttgaaacga ccgtccaggt ttcgggcacg gtggcgacca tttcggaggt
     6421 ggcgcggtcg ttaggaaaat cccagcaggg gaaccagaag tggttgtctt catcttcccc
     6481 ctgactccag atctgtttgg gcttggtggg ataccccgaa tcgggaccga cgaagtagag
     6541 ccccttacgc ggcttggccc agtaggcaac gctgaattca atcggttttc cggcgtcata
     6601 cgcttccggg agtttcacga cgagaaacga atcatccgcc gcatagacca gagatttttg
     6661 cgccgcatcg gtgacgccgg taatattgag gtcgccctgg ttcagccgga cttcccggaa
     6721 accggagttg aggggtacca aacggtacgt taccttcccg ctgacttttt tctggtccca
     6781 gtcgaaggtg agatcgagcc ggatgtgctg gaggtcgtac gagcggtcgg gcggccagtg
     6841 gcgggtggag agttcgtcgc cgaacggctt ataacggtcg gctgaagcgg ggaaagcggc
     6901 ggttacgacg aagaagattg tccaactcag gaacttgctt gcgagttgta ttcgcttcat
     6961 ggaatctcca ccagttctat gtgcagttcc ggatcggtct gcagccggcc gtaggtcaat
     7021 gtgcggcggt caaagttaat tgagagactg ccgggattaa agagcaaagt cgctccgaac
     7081 ttcttggaaa aaggggtgtg cgagtggccg aagacgatga tgtccaccgg ctcggtgaat
     7141 tcggcccgga cacggtcgac caatccctcg ggggcaccgt ggccgtggta caatccgatt
     7201 ttgaccttcc ccagcgtgac gaccgttttg cggggcaatt cttttcgcac ctcaggaccg
     7261 tccatattgc cccacacggc gtggactttg gcccggcggc gcaactcctc cagcaccacc
     7321 ggcgtggtga aatcaccggc gtggagcacg atatcctccg gagtaaggcg ttcgagaaat
     7381 ttcttcggga tcgccttgac ccggtcgggg atgtgcgtat cggaaatgat gtaaacagcc
     7441 atccagcccc tctttttcca gagaaaaaga ggaatgcgaa cctactactg tacgcgacgg
     7501 gctctaccgc agttttttct tatggcggtt cttgcggcgg cgtttcttcc gcttgtgggt
     7561 gcggatcttc tgtaacttgc gtttccttcc gctgggcaca agcacctcct cgtgattaca
     7621 ccggctgctt gatgatcaaa cccttaaatt cattggaagg tttgaaaacc ggcactttcc
     7681 ggtcgggcac cggcacctcg tcgccggtgc gcgggtttct ggctttgcgc gccttgcgca
     7741 ctttcacctt aaaggtgcca aagccgcgaa tctcgatgtt gctgcccccc tccagggcgg
     7801 ttttcaccgc ttccaaaaac gaatcgacga ccacggccac gtcggtccgg gtcagtcccg
     7861 tcttgccggc tacctgctca accaaatcgg ctttggtcat gctccctcct ggaatcctgg
     7921 cttagatcgc ttacccacaa aatcggtcac agtggccgct gattttttca cgttcagtcg
     7981 agattgtctt ggcaatgccc gcagccttcc ggcgggcagg cgtccgctcc gctatctt
//
LOCUS       DBNGZA010000270        13773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2592629, whole genome shotgun sequence.
ACCESSION   DBNGZA010000270 DBNGZA010000000
VERSION     DBNGZA010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2592629"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /gene="ispD"
                     /locus_tag="AC16FU_05690"
     CDS             complement(<1..1021)
                     /gene="ispD"
                     /locus_tag="AC16FU_05690"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR2993898.1"
                     /translation="MQKISAIVVAGGTGSRFGNGLPKQFARLGDRPILAHTLGKFQLV
                     EQVAEIVAVVPTEWESYCRDEIVTPFNLTKVARVVTGGATRQESVARGLAAVAPDTTT
                     VLVHDGVRPLVSPEKIAAVCDALEHSPACLLVLPVQETLKEGEGGAVRKTLERQGVYL
                     AQTPQGFHKSLLAQALAQAEKDHFTATDDAALVERLGVTPQIIAGEPTNLKITTSEDL
                     AFAERLFGLPVTQSRVGFGYDSHRLVAGRPLILGGVTIPFEKGLAGHSDADVLCHAFA
                     DALLGSLGLGDIGSHFPDTEEKWRGVSSLEILRQVAVKVAASHGEITSVDATVVAEKP
                     RLGPYI"
     gene            complement(1268..2449)
                     /locus_tag="AC16FU_05695"
     CDS             complement(1268..2449)
                     /locus_tag="AC16FU_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR2993899.1"
                     /translation="MKTLDKVIGLGGEAENIGRFLQDVRERKALSQSTVAERAHITQS
                     ELSKIERGKHLPNLDTLRRIVAALGETPLGIRALWAEIGDERTSVEVTPQDLKEGQNF
                     DHAKEEAKRLMYEGNLFHASKKIAVMERLAKTLDERVRAGLYAGYYAREAGFYADSDE
                     YFREAVKALTDVDEKSVLWDMYYVNHASVLTYTGNLPWAEMLLDKLMARNPGDNFYHR
                     WGVFERGRLALAREDAQKAKALFKSAIHHFDECREKDERLLPCHLGYFQLFLAKSMLT
                     SDEEKEKACMLLQHLMECWGPDENRPRNAEIYAEAALEYGLATNKEAFLEKAKDVAKK
                     NGLIRIVRIVQKHQPEDENRNNPKDGASRVPIILSSLAFFLLFSYFVLLPLAQAMCAI
                     E"
     gene            2720..3295
                     /locus_tag="AC16FU_05700"
     CDS             2720..3295
                     /locus_tag="AC16FU_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993900.1"
                     /translation="MMKLKTAGVLLGGLLLLVAPAITLGQSGSYGDQVEDGIDMAVAP
                     DAIDPNPCDLNPCIGPPVEPVYPEPYFDWDGWDRLFRLDGEEPPDDPFNFDPDIEAEP
                     LVDNGPDQEGLSVADNSGGNTTDETMDNGANRAEPTHDTVAGGETGNGETAGGGDGND
                     PGGGDGPDGPDGGDHDGGGGDDGGSDGGDVG"
     gene            3961..4476
                     /locus_tag="AC16FU_05705"
     CDS             3961..4476
                     /locus_tag="AC16FU_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR2993901.1"
                     /translation="MTVGHQLQNADYHLKPAELKKVIYAPLLSFRDRTILKTFAQTGI
                     RRAELAALDVRDIDFERRTLEIRAGKGSKSRTLPLSEDLASDLRHLVGRRKTGPVFVS
                     QRGSFLAPRQLNWIVARAGELAGVKDPNPRARHLHIHTFRTTAAAPACPAEVLSADRR
                     FRWQSGPAASG"
     gene            complement(4382..5371)
                     /gene="ruvB"
                     /locus_tag="AC16FU_05710"
     CDS             complement(4382..5371)
                     /gene="ruvB"
                     /locus_tag="AC16FU_05710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR2993902.1"
                     /translation="MEGELEYDLSLRPTRFEEFVGQKKTIENLKIFIAATKKRGEALD
                     HELFYGAPGLGKTTLAYIIAKELGVPIKVTSGPVLERAADLAGLLTNLSEHEVLFIDE
                     IHRLNHVVEEYLYGAMEEFALDIMIDRGPAARSVRLPLKKFTLIGATTRAGLLTSPLR
                     ARFGVVGRLDFYQPEDLFQIIQRSAKILSVQIDEAGALEISSRSRGTPRVANRLLRRV
                     RDFAQVESNGKITKAVAQDALARLDVDERGLDEMDKRLIEVIISKFKGGPVGINTLAV
                     AVGEEEDTIEEVYEPFLIQEGFINRTPRGRFATETAYRHLGLLPDKQAQPRLF"
     gene            complement(5491..6087)
                     /gene="ruvA"
                     /locus_tag="AC16FU_05715"
     CDS             complement(5491..6087)
                     /gene="ruvA"
                     /locus_tag="AC16FU_05715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR2993903.1"
                     /translation="MIAYLEGRLVEKQPTAVVLDVNGIGYRVQIPLSSFSALAGAGAN
                     GRVRLFTSFHVREDSHQLFGFATQAEREMFELLLSVSGVGPKAALALLSGLALPTLRR
                     AIAEQNTLTLESVSGVGKKLAARLTLELKDKIGKLSAITGDGALAQKPTDSQEEAVLA
                     LVSLGYTRPEANRKVEQVAKRKDLPVEEVVRQALQTAG"
     gene            complement(6084..6704)
                     /gene="ruvC"
                     /locus_tag="AC16FU_05720"
     CDS             complement(6084..6704)
                     /gene="ruvC"
                     /locus_tag="AC16FU_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR2993904.1"
                     /translation="MGKKFDPTCGVRIIGIDPGLLATGYGVIEVNDLPRENQFRLVCA
                     GALRTSAKISTAERLKQLFDGLCALLSEVRADAAAVEEAFYAQNVRVALTMGHARGAT
                     LVALGSQHLPVYEYATREVKQAVTGQGAAAKGQVQFMVGKLLGCDLSREAHDVTDALA
                     VALCCALRTKAAHSTRHTALRKPLIPKSEILTPMRFDYAHHKGLHG"
     gene            complement(6724..7473)
                     /locus_tag="AC16FU_05725"
     CDS             complement(6724..7473)
                     /locus_tag="AC16FU_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR2993905.1"
                     /translation="MSGHSKWATIKHKKEKTDAQRGKLFTKLIKELTVAARHGGGDPE
                     ANPRLRTAVAAARAANMPTENIKRAIQRGTGELPGVSYEEITYEGYGPGGVALLVEVV
                     TDNKNRAVSDIRHLFTRNHGNMSEAGSVAWMFERRGLIQVDSQKIGEDDLLAVALDAG
                     AQDMSREGEFYEIISPVQQFETVRAALEKSRIPIAQAEITLVPQTTVRLEGAQAEQML
                     KLIEALEDHEDVQKVFANFDIAEEVMEKLSA"
     gene            complement(7563..8477)
                     /locus_tag="AC16FU_05730"
     CDS             complement(7563..8477)
                     /locus_tag="AC16FU_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR2993906.1"
                     /translation="MFSFSTSWNAGRHSSGRELVAEIRELGFSAIEVNYRVRARALAD
                     IKALVRAGEVSVPSLHNAVPCSEDQDLATAHSAFPLSSPEKKVRQRGIDLARWTLDHA
                     CELGAQAVVLHLGEVEMPGAENLEREYRLFLKDGKTDQAAHFKQKMIAERSRHAAGFF
                     GRALESLHRIALQALTCGVRLGIENRYHWFQVPSFDELQEIFRQFPGETVGYWHDVGH
                     AQTMENTGFTAHLAYLDAFGPRLVGCHLMDCVFDRDHLACGAGEIDFSVIAPYLRDSV
                     LKVFELGQAATPEDIAASVPYLAKFGIQ"
     gene            complement(8664..9269)
                     /locus_tag="AC16FU_05735"
     CDS             complement(8664..9269)
                     /locus_tag="AC16FU_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2993907.1"
                     /translation="MRRFSALTGLATLTLFLASSGWAGYGQGQNAKGQLAFVLRGGAG
                     IPVFDLRTLNIYGQKTGAAFGAQGEYFVKDGLAVGVDVGYTVFSKRTEDNLPRSAVRP
                     WHFLAHLKYYLPVGGKIGPYLEYGAGANHFTFSENVKSQTKFALLAGAGLDIAVRPDF
                     ALVLGGTFDYAIFGKQARDVRSQQVGWFSLLGGVRFTFAAE"
     gene            complement(9309..9887)
                     /locus_tag="AC16FU_05740"
     CDS             complement(9309..9887)
                     /locus_tag="AC16FU_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2993908.1"
                     /translation="MRHKFCTVLAIVLLALPGLSWAAHQKGNLGVFVLGTGATGTSDN
                     LKNFTANGGGLGLGAEVYFANHLAFGVQGSATLLPGFQLGGQTTNDWWFWEYGGYLKY
                     AIPALNWFSPYVKGGFGGATITFHESGPTKTPNQTRPVLYAGTGVTLEVSPRWSLFAE
                     ALFHDAMTAGAGPAHWIAGGAGATYWFGLPKP"
     gene            complement(9948..10535)
                     /locus_tag="AC16FU_05745"
     CDS             complement(9948..10535)
                     /locus_tag="AC16FU_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR2993909.1"
                     /translation="MRKVIMLVLAGAVLGSNVATAKEMKGKFALGLNLGANIPTGDFG
                     NEFKVGGVADLTGEYYLSNVVALGVEVEGSVNDPKSDVAAQFPGVDISATISSFNATG
                     RFFFPSQSVEPYVIAGLGAYTQELDLEVSGFVVKVSDTDFGIHGGAGINFPVSPKVGI
                     LAEADFHNIFSKGSSTNFVNVKAGVRLLLGGKKSP"
     gene            complement(10640..11323)
                     /locus_tag="AC16FU_05750"
     CDS             complement(10640..11323)
                     /locus_tag="AC16FU_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993910.1"
                     /translation="MGKKILVGGTVVMAIFLAAGAFAQPASPIAKGKVLFSVQGTELS
                     VLAVDTLGFAKGLSGDADAGEPSFFPSASLVGGRATVALGLSDQYALNAAFLWAGGFQ
                     KTVPADTGESESKGTITALGVRVGLDRHVPVAKWVSLYGGGGVLWTSTQVKIRVNQGP
                     TSVEYKDPRTSSFSLSGRMGFFVRFKESFGLYGDLGQQLSWVSKKVRGKATALTSSHD
                     GNIGIAVLF"
     gene            complement(11424..12014)
                     /locus_tag="AC16FU_05755"
     CDS             complement(11424..12014)
                     /locus_tag="AC16FU_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2993911.1"
                     /translation="MRKIILFVTGAVLLLTIPAFAQYAGKSAEKGKWSVAPFGNVSFP
                     MGSLKDFTKTGFGGGLAAEYYVADAFAVGVQGSYNTFSGDPSPLPDWRPWQYGAFGKY
                     VIPTQSKLSPWIKYGAGGYSLTFSKVAGATTTAPSQSRVGWFGGLGVAIEATPTVSVF
                     VEGLYHDALKKHELPVNWVSAGVGVQFLLGMGSTAK"
     gene            complement(12209..12892)
                     /locus_tag="AC16FU_05760"
     CDS             complement(12209..12892)
                     /locus_tag="AC16FU_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993912.1"
                     /translation="MRAKAKTKVWPRVVVGTTLALLGAAGAVHGQEAKGKGAVVVGFG
                     TGPALPIGLSNTPNVPDDFPDAYGSGFGGSASLSYYVHDRIEVGLAAGLNWHHPTDGF
                     DQLYQLSGNTAAPDVSIIPVVGFIKTDLAPQKKLTPYVRVGGGLYRLQFGSVTTAGGA
                     FKSVAAVNKSGVFAAGGFQLDLVPAVQVFLEGAFHNIFKFDFFNVDPSKSFGLVTVQG
                     GLRLGLGRP"
     gene            complement(12957..13604)
                     /locus_tag="AC16FU_05765"
     CDS             complement(12957..13604)
                     /locus_tag="AC16FU_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR2993913.1"
                     /translation="MRRLVVVCLLAGIVGLGWTGAGGAELKGKVTIAPALGLLTPVGD
                     FNDQMDMGFTGGAQVEYYFSNLVGFGLTASGNFFPGVARPTGILPSGLRDLPDPDTTL
                     AQFGIFASLHLRPAQRLSPYFKGGWTMNVIFESNSFEDQEGARIDTVTTHHTETDFGV
                     FGGAGLSYDLTERTSFFTEAVVHDLFRLDETINEFTGRQYVQVWVGARFTFDWFK"
BASE COUNT         2901 a         4314 c         3663 g         2895 t
ORIGIN      
        1 caatgtacgg ccccaaacgt ggcttttccg ccactaccgt tgcatcaacg cttgtgattt
       61 ccccgtggct ggcggccacc ttgaccgcca cctgccgcaa aatttccaga ctcgaaacgc
      121 cgcgccactt ttcctccgta tctggaaaat gtgacccgat gtcgcctaag cccaaactgc
      181 ccagcagggc gtcggcaaag gcgtggcaga ggacgtcggc gtcggagtgc ccggccagcc
      241 ccttctcgaa aggaatcgtg actccgccta aaatcagagg ccgtcccgcc accagccgat
      301 ggctgtcgta cccaaagccg acgcggcttt gtgtcacagg cagaccgaaa aggcgctcgg
      361 cgaaggccaa atcttcagac gtggtgattt tcagattcgt cggctcccca gcaatgattt
      421 gtggagtgac tccgagacgt tcgactaacg ccgcgtcatc agtagcggtg aaatgatcct
      481 tttccgcctg cgctagggcc tgcgccagca aactcttgtg gaacccctgc ggcgtctgtg
      541 ccaggtaaac accctgacgt tctaaagttt tccgaaccgc tccgccttcg ccttccttca
      601 acgtctcttg aaccggcaaa accagaaggc aggcgggtga atgctccagc gcgtcgcaga
      661 ccgctgctat tttctccggc gacaccagcg gccgcacgcc gtcatgcacc agaacggttg
      721 tggtatccgg cgccaccgcc gccagcccgc gcgccaccga ctcttgcctc gtcgccccgc
      781 cggtgacgac gcgcgcaact tttgtcaggt tgaatggagt cactatctca tcccggcaat
      841 agctttccca ctccgtgggc acaacggcga caatttcagc gacctgctcc accaactgaa
      901 atttccccaa tgtgtgcgct aagataggcc ggtcgcctaa gcgggcaaac tgttttggca
      961 ggccgttgcc aaaacgactc cccgtcccac cggctaccac aattgccgag atcttctgca
     1021 cggaggtaaa gaaaacactt atttgcggaa gaacaaattc aaaaggagag ttaaagccag
     1081 caccaatacc cgccgacggc gggatcgaag gccgccggta gcgaagaagc ggagcgggca
     1141 acccccgctc cgcctttttc gtgctcgcgg aaaaatcctt cccacccctt gcagtcatgg
     1201 tgtattgtag aggctaataa aaaaagcgct gagaggaaaa cccctcagcg catttcactg
     1261 gtctgagtca ttcaatagcg cacattgcct gagccaaagg caatagcacg aagtatgaga
     1321 acaacaagaa aaatgccaga gacgacagta ttatgggtac cctgctggcc ccatccttcg
     1381 ggttgttcct gttttcgtct tccggctggt gtttttgaac aatccgcact atcctgataa
     1441 gcccgttttt tttcgcaaca tcctttgcct tctccaaaaa agcctccttg ttggttgcaa
     1501 gtccatattc cagagccgcc tcggcataga tttctgcatt acgtgggcgg ttttcgtccg
     1561 gtccccaaca ctccatcaga tgttgtagga gcatacatgc tttctccttc tcttcatccg
     1621 aggtgagcat cgacttcgcg aggaaaagtt gaaagtaacc caaatgacag ggcagcaacc
     1681 tctcatcttt ctctctgcat tcatcgaaat ggtgaatggc agatttaaaa agagctttcg
     1741 ctttttgggc gtcctctctt gcgagcgcaa gtcgcccacg ctcgaatacc ccccagcgat
     1801 ggtaaaaatt atcgccggga tttcgggcca taagcttatc caataacatc tccgcccaag
     1861 gtaggttacc cgtgtaggtt aaaacgctgg cgtgattcac gtagtacata tcccaaagca
     1921 cgcttttctc gtccacatcc gtaagggcct tcacggcttc acggaaatac tcgtcggaat
     1981 ccgcgtaaaa tccggcttcc ctagcgtagt acccggcata gagccctgct ctcaccctct
     2041 catccagagt tttagctaag cgctccatga ccgcaatttt cttcgatgca tgaaaaagat
     2101 tcccttcata catcaatctt tttgcctctt ccttagcatg gtcgaagttc tgcccttctt
     2161 taaggtcttg gggcgtgacc tcgacgcttg tacgttcatc cccaatttcg gcccacaacg
     2221 cgcgtatccc cagaggagtc tcgcccagtg cagctacgat tcttctcaaa gtatctaagt
     2281 taggcaagtg cttccccctt tcgatcttgg acagttctga ttgggttata tgtgcccgtt
     2341 ccgcaaccgt tgactgggaa agtgctttcc tttcacgcac gtcctgcaga aaacgcccga
     2401 tattttctgc ttcccctcca aggccgataa ctttgtcgag ggttttcatc gttggtctcc
     2461 tttccttaaa tggttttagc cgctccataa ttttctctcc attaattaat acgtttgaac
     2521 acccgtggag agcattcaca gaccttgtaa tccacgcata gcccccattc tattgttaca
     2581 taatgattta tagcccaatc cccctgagtg gttcgtataa agaattggta tgaataggca
     2641 gatgcgcgca gccagccagt tgacccgacc ggggccgggg ttcgctggga aaaacacagg
     2701 ccccagatcg gagagcaata tgatgaagtt aaaaacggcc ggcgtcctac tgggaggact
     2761 cttgcttttg gtcgccccgg ccatcaccct gggccagagc ggttcgtacg gtgaccaagt
     2821 tgaagatggc attgatatgg cggtagcgcc ggacgccatc gacccgaatc cctgcgacct
     2881 aaacccgtgc atcggccccc cagtggagcc ggtttaccca gagccatatt ttgattggga
     2941 tgggtgggac cggttgtttc gcctcgacgg ggaggagcct ccagatgacc ccttcaactt
     3001 cgacccggat atagaagcag agccgttggt cgacaatggg ccagaccaag aaggcctctc
     3061 ggtagcggac aactccggtg gtaacaccac ggatgaaacc atggacaacg gggcaaatcg
     3121 ggctgagccg acccacgata cggtagcggg cggtgagact ggaaatggag agaccgccgg
     3181 tgggggcgac gggaatgacc cgggcggggg tgatgggccg gatggtcctg atgggggcga
     3241 ccacgacggc ggaggcgggg acgatggtgg ctccgacgga ggagatgtcg gttagctaaa
     3301 ataactgact acccgcaggg ggagggcaaa accttccggg tagtcaaaca aaaacttgag
     3361 aggaggtgaa aacaaagatg aactcgaaga ttggcatttt tagattggtc accgcactga
     3421 cggtaggggt tggcctttac gggttttcca aggtaccgga cacaacgttc acccatacgt
     3481 ccaacccgga cctggtcgat acgaattcta attcttcgta ttatccggac ccgagcgacg
     3541 cgaacatgtt gacagatgag ccgtgcccaa ttgacacggt ttatttgcca ttgccgtgtc
     3601 cttcgcccga aaccgtgctc gttgtagttc acgatacagt ttttattggg gacgagcggg
     3661 atggggatga ctacattaga tggccggacg accgccatgg catctggccc gacgacggaa
     3721 ggccggactg gtggtacgat acgacgcaaa cagaggattg gaacctttgt tgaacctcgc
     3781 tgttgtaccg taaccagcgc actgaaagcc tacgcattta acagcgtagg ctttcttaat
     3841 ttacggcaaa cattgcctac cttatacaga atgtgctatc tttcggcttg acagctaccg
     3901 gccgcagacg ctatccttgc cccggatgat aagtttttca cttcttttag gtaaggtgct
     3961 atgacggtcg gccatcagct gcaaaacgct gactaccact tgaaaccggc ggagttgaaa
     4021 aaggtcatct acgctccgct tctgtccttc cgggaccgaa ccatcctgaa aacttttgcc
     4081 caaactggca tccgccgggc ggagctggca gcattggacg tccgagacat cgacttcgag
     4141 cgccggacac tcgaaatccg cgccgggaaa ggttccaaga gccggacgct gccgctttcg
     4201 gaagatttag ccagcgattt gcggcacttg gtcgggagac gaaagaccgg tcccgttttc
     4261 gtatcccaac gaggcagctt ccttgcccca aggcagctga actggattgt tgcccgggct
     4321 ggggaactgg ccggagtgaa agacccgaac cctcgggcgc gccacctgca tatccatacg
     4381 ttcagaacaa ccgcggctgc gcctgcttgt ccggcagaag tcctaagtgc cgataggcgg
     4441 tttcggtggc aaagcggccc cgcggcgtcc ggttgataaa cccttcctga atcaaaaacg
     4501 gctcgtacac ttcttcaatc gtatcctctt cttcgccgac agcaacggcg agcgtgttga
     4561 tccccaccgg cccgcctttg aacttggaaa ttatgacctc gatcaaccgc ttgtccatct
     4621 cgtccaatcc gcgctcgtcc acatcgagcc gtgccaaggc atcctgcgct acggccttgg
     4681 tgatcttccc gttcgactcc acctgcgcaa aatcgcgcac ccggcgcaat agccggttgg
     4741 ccacccgggg cgtcccccgc gagcgggagg aaatttccag ggcgcccgct tcatcgattt
     4801 gcacgctcag aattttggcc gaccgctgaa taatttggaa gagatcctcc ggctggtaga
     4861 aatccagccg ccccacgacg ccaaagcgcg cgcgcaaagg cgaagtcaga agcccggccc
     4921 gggtggtggc gcctattaag gtgaattttt taagcggcag gcgcaccgag cgcgccgccg
     4981 ggccgcggtc gatcataata tctaaggcga actcctccat cgccccgtac agatattctt
     5041 cgacgacgtg gttcaaccgg tgaatctcgt cgatgaagag cacctcgtgc tcggaaagat
     5101 tggtcaaaag ccccgctaag tcggccgccc gctccaacac cggcccggag gtcaccttga
     5161 ttggtactcc taattctttg gcgatgatgt acgccagggt cgtcttgccc aagcccggcg
     5221 cgccgtagaa gagctcgtga tccaaggcct cgccgcgctt tttcgtcgcg gcgatgaaga
     5281 ttttcaagtt ttcgatggtt ttcttctgcc cgacgaactc ctcaaaccta gtgggccgga
     5341 gcgacaggtc gtattctaac tccccctcca tccgcccggg cgcggtgacc cgttctttgg
     5401 tctggccttc gcgcggcaaa accagtttcc gcccggcgcc tcgctcggta ttgggtctaa
     5461 agcttccaga acctttgaac ggttgcgcca ttatcccgcc gtttgcaagg cctgacgcac
     5521 tacttcctct accggcaggt ctttgcgttt agctacctgc tccactttcc gattggcctc
     5581 gggccgcgta tagccgaggg aaaccagagc taaaaccgcc tcttcctgac tgtcggtcgg
     5641 cttttgcgcc agggcaccgt caccggtgat ggctgacagt ttgccgattt tgtccttcaa
     5701 ctccaatgtc aatcgcgccg ctaatttttt tcccacgccc gaaacgcttt ctaaggtcaa
     5761 cgtattttgc tcggcaatcg cccgccgcag cgtgggcaaa gcgagacccg acaaaagcgc
     5821 cagcgccgct ttcggaccga cgccggagac ggagagcagc aattcaaaca tctcccgctc
     5881 cgcctgcgtg gcaaagccga acaactggtg ggaatcctcg cgcacgtgga atgacgtaaa
     5941 gagcctcacc cggccgttgg cccccgctcc ggctaaggcg gaaaaactgg aaagcggaat
     6001 ctgcactcgg tagccgatcc cgttgacgtc gaggaccacc gctgtgggtt gcttttccac
     6061 cagacggcct tcgaggtagg cgatcatccg tgcaacccct tgtggtgagc gtagtcgaac
     6121 cgcatcggag ttaggatttc ggatttagga attaggggtt tcctgagtgc tgtgtgccgt
     6181 gtactgtgtg ctgccttcgt tcggagcgca cagcaaagcg ctaccgccaa agcatcggtg
     6241 acgtcatgcg cttcccgcga caaatcgcag cccagcaatt tccccaccat gaactgcacc
     6301 tgccccttgg ccgccgcacc ctgcccggtc accgcctgct tcacctcgcg ggtggcgtac
     6361 tcgtagaccg gcaggtgctg gctccccaga gccaccaggg tcgctccccg ggcgtgcccc
     6421 atggtcagcg ccacccggac gttctgcgcg taaaacgctt cttccaccgc cgccgcgtcg
     6481 gcccgcacct cactcaaaag cgcacagagg ccgtcgaaca attgcttcag ccgctccgcc
     6541 gttgaaatct tggcgctggt gcgcagggcg ccggcgcaaa ccagacgaaa ctgattctct
     6601 cggggaaggt cgttaacctc gatcacaccg tagccggtgg ccaaaagccc cggatcgatc
     6661 ccgatgatcc ggacgccgca agtcggatcg aacttttttc ccatcccgcc tcaagccgag
     6721 ctcctatgcg ctcagctttt ccatcacctc ttcagcgatg tcaaagttgg caaagacctt
     6781 ctgcacgtcc tcgtggtctt ctaaggcctc gatcaacttg agcatctgct ccgcctgcgc
     6841 gccttcgagc cggacggtcg tctgcggcac cagggtgatt tccgcctgcg caatgggtat
     6901 gcggctcttc tccagggctg ctcttaccgt ctcgaactgc tgcaccgggg aaatgatttc
     6961 atagaactcg ccctctcgcg acatatcctg cgctccggcg tccagtgcaa cggctaaaag
     7021 gtcgtcttcg ccgattttct gggaatcgac ctgaatcaac ccgcgccgct cgaacatcca
     7081 ggccaccgaa ccggcttcgc tcatattccc gtggttgcgg gtaaagaggt gccggatatc
     7141 ggagactgcc cggttcttgt tgtcggtgac gacctccacc aagagcgcca cacccccggg
     7201 gccgtatcct tcgtaggtaa tttcctcgta ggagacgccc ggcagttcac cggtcccccg
     7261 ctggatggcc cgcttgatat tctccgtggg catattggcc gcccgggcgg ccgccacggc
     7321 cgtgcgcagt cgcgggttcg cctccggatc ccctccgccg tgccgcgccg ccacggtgag
     7381 ctccttaatc agtttagtaa agagttttcc gcgctgggcg tcggtcttct cttttttgtg
     7441 cttgatcgtg gcccatttcg aatggccgga catatcctca ccttctggtt ggcacagaac
     7501 ttacttcacg tttcgttccg caagtgaata ataaatacaa acacaccaag aaaagcaatt
     7561 tcctactgaa tgccgaattt tgccaggtag ggaacgctgg cagcaatatc ctcgggggtg
     7621 gcggcttgtc ccagttcaaa aactttcagg acagaatccc tcaaataagg agcgatcacc
     7681 gaaaaatcga tctcgcccgc cccgcaggcc aggtggtcgc ggtcaaagac gcaatccatc
     7741 aggtggcagc cgacgagacg cgggccgaat gcatcgaggt aggcaagatg cgcggtgaag
     7801 ccggtattct ccattgtctg cgcatgcccc acgtcgtgcc aataacccac ggtctccccg
     7861 ggaaactgtc ggaagatttc ctgcagctca tcaaacgacg gcacctgaaa ccagtggtag
     7921 cggttttcga tccccagccg aacaccgcag gtgagcgcct gcagggcgat acggtgcagc
     7981 gactccagcg ctctgccgaa aaatccggcg gcgtgccgcg accgctccgc gatcattttc
     8041 tgcttaaagt gtgccgcctg atccgttttc ccatctttca ggaagaggcg gtactcgcgt
     8101 tctaagtttt ctgcgcccgg catttccacc tctcctaagt gcaacaccac cgcctgcgct
     8161 cccagttcgc aggcgtggtc aagcgtccac cgcgccaggt caatcccgcg ctggcggacc
     8221 ttcttctccg gtgaggaaag gggaaaggcg ctgtgggcgg tggctaagtc ttgatcctcc
     8281 gaacagggta cggcgttgtg caaactggga acgctcactt ccccggcacg caccagcgct
     8341 tttatgtcgg ccagcgcccg cgcccggacg cggtagttga cctcaattgc ggaaaatcct
     8401 aattcccgga tttcggcaac cagctcccgg cccgacgaat gccgccctgc attccagctg
     8461 gttgaaaatg aaaacatctg cggaaattac cggaaaactc gcatccgggg tagccccggt
     8521 ctctttagcc cgagtccctc cacaggcggg atcaaagacc cgcccggcgg aaatgttttc
     8581 agactgaacc ggtacgtttt cgaaagtcta ctcgggggaa tatcctatcc atcggtcagg
     8641 cttcccagcc tgactactga ttcttactcc gccgcaaacg taaagcgcac cccacccaag
     8701 aggctgaacc agcccacctg ctggctgcgc acgtccctgg cctgcttccc gaagatggcg
     8761 taatcgaagg tgccgcccag aaccaaagcg aaatcggggc ggacggcaat gtccaaaccg
     8821 gcaccggcca aaagcgcgaa tttcgtttga ctcttcacat tttccgagaa agtgaaatga
     8881 ttcgccccgg cgccgtactc caggtagggt ccgatcttcc cgcctaccgg caaatagtac
     8941 ttcaggtgcg ccagaaaatg ccacggccgg acggccgagc gcggcagatt gtcttcagta
     9001 cgcttggaga aaaccgtgta tcccacatcg acgcccacgg cgagaccgtc tttgacgaaa
     9061 tactctcctt gagcgccgaa cgccgctccc gtcttctgcc cgtaaatatt gagcgtccgg
     9121 agatcaaaga ccggtattcc cgccccgccc cgcaggacga aggccagctg ccctttggcg
     9181 ttctgcccct gaccgtagcc cgcccacccg ctggaggcca ggaataatgt aagtgtggcc
     9241 aaccccgtca gcgcagagaa tcttctcatg acccgctcct ccaggaagac ccgatacctg
     9301 ttagccgact acggcttcgg taagccaaac cagtaggttg ctccggcacc gccggctatc
     9361 cagtgggccg gcccggctcc ggccgtcatc gcatcgtgga acaaggcctc ggcgaaaagc
     9421 gaccagcgcg gtgaaacctc aagcgtcacg cccgtcccgg cgtagagcac cggccgcgtt
     9481 tgattgggcg ttttggtcgg accggactcg tggaaagtga tcgtcgcgcc gccgaaaccg
     9541 cctttgacgt acgggctgaa ccaatttaga gccggaatgg cgtacttcag atagccgccg
     9601 tactcccaga accaccagtc gttggtggtc tgcccgccca actgaaaccc cggcaggagt
     9661 gtggcgctgc cctggacgcc aaaggctaaa tggttcgcaa agtacacttc ggcacccagg
     9721 ccgagcccgc cgccattggc cgtaaaattt ttcagattgt cggaagtccc cgtcgcgccg
     9781 gtgcccagaa cgaacacacc cagattgccc ttctggtgcg ccgcccaact caaaccgggt
     9841 agcgccaaga gaacaatcgc cagaaccgtg caaaacttat gacgcatccg tctcctttct
     9901 gaaaaagagg cgctacatcc cgagaggaat gcggcgcttt gatcgactta cgggctcttt
     9961 tttccgccca ggagcagccg cacgccagct ttgacgttca cgaaattggt ggacgacccc
    10021 ttggagaaga tgttgtgaaa atcggcttcc gccaggatac ccactttggg tgagacgggg
    10081 aaattgattc ccgcgccccc gtgaattcca aagtcagtgt ctgaaacctt gacgacgaag
    10141 ccggatacct ccaaatccag ttcctgtgtg tacgccccta agcccgcaat cacatacggt
    10201 tccacgctct gcgaggggaa aaaaaaccgc ccagtggcgt taaacgagga aatcgtagcg
    10261 gaaatgtcca caccgggaaa ctgcgccgcc acgtccgatt tcggatcgtt cacgctccct
    10321 tccacctcta cacctaatgc cacaacattg ctcaaatagt actccccggt gagatcggcc
    10381 accccgccaa ccttgaactc gttgccgaaa tcgccggtcg ggatgttcgc tcccaggttt
    10441 agtcctaagg caaacttacc cttcatttcc ttggcggtcg ccacattgct gcccaaaacc
    10501 gcacccgcca ataccaacat gatcactttc cgcatggtct gcctcccgtc ctgtttccga
    10561 ggccatctgc ccctcggaga tctgtgaaag cgagaacgct gtgccctttc cgggcgcggc
    10621 gccaggtatc ataccaactt tagaacaaca ctgcaatgcc gatgttgccg tcgtggctgg
    10681 aagtcagcgc ggtggctttc ccccgcacct tcttggaaac ccaggagagt tgctgtccca
    10741 ggtcgccgta caacccgaag ctctctttga accggacgaa aaaacccatc cgtccggaga
    10801 gcgaaaatga actggtacgc gggtctttat actccaccga ggttgggcct tgattaacgc
    10861 ggattttgac ctgtgtcgag gtccacagca ctcccccgcc gccgtagaga gaaacccact
    10921 tcgccaccgg cacgtgccgg tccagcccca cccgcacgcc cagggccgtg atcgtgcctt
    10981 tggattctga ttctcccgta tcagcaggca cggtcttttg aaatcctccc gcccagagga
    11041 aggcagcgtt gagcgcatac tgatcactca gaccaagggc cacggtcgct ctgccgccaa
    11101 ccagggaagc cgacggaaaa aaggatggct caccggcatc ggcatcaccc gataagcctt
    11161 tagcaaaacc taacgtgtcc accgccaaaa cggagagttc ggttccctga actgaaaaga
    11221 gcactttgcc cttggcaatc ggactcgcgg gctgggcgaa agccccggcc gcaaggaaaa
    11281 ttgccatcac taccgttccg ccaaccagaa tctttttccc cacgattccc ctcccgtcct
    11341 gtttccgagg ccatctgccc ctcggagatc tgtcaaagag agaacgccgt gcccctactg
    11401 gggcacggcg tccgtgaaca ccgctacttg gccgtacttc ccatcccgag caagaactgg
    11461 acgcctactc cggcactgac ccagttgacg gggagctcgt gctttttgag ggcatcgtgg
    11521 tagagaccct cgacaaagac cgagaccgtc ggagtggctt caatcgccac gccgagcccg
    11581 ccgaaccatc cgacgcggga ttgactcggc gcagtggtag tggccccggc gacttttgag
    11641 aaggtgaggc tgtagccgcc cgcaccgtac ttaatccagg gcgagagttt cgactgggtt
    11701 gggatcacgt acttcccgaa agcgccgtac tgccagggac gccaatccgg gagggggctg
    11761 ggatcgccgg aaaaggtgtt gtaactcccc tggacaccga ccgcaaaggc gtcggcgacg
    11821 tagtattccg ccgccagccc gccgccgaat cctgttttcg tgaagtcttt cagtgatccc
    11881 atgggaaaac tgacgttgcc gaagggcgcc accgaccatt tccctttctc cgccgacttg
    11941 cctgcgtact gcgcgaaggc cggaatggtc aggagcagca ccgctcccgt cacgaacaaa
    12001 atgatcttcc gcatcttact cctcctgttt ggtttacctc ttcgactccg attacagata
    12061 ccaaaccgct gctaatcagt cttttacctc acctccttgt tcactcccgg gtttactccc
    12121 cggggcttcg accgctggtt ctatggcaat ctctagtaaa catttccaca tacggctacc
    12181 cgcaaaaaat gttgaggagt tttttatgtc agggtctccc caatccgagc cgcagtccgc
    12241 cctgcacggt gacgaggcca aaagacttgc tcgggtcaac gttaaagaaa tcgaacttga
    12301 agatgttgtg gaaggcacct tcgaggaaga cctgcaccgc cggaaccaga tccagctgaa
    12361 acccgccggc ggcgaatacg ccgcttttgt tcaccgctgc gacggacttg aacgctccgc
    12421 ccgcggtggt gacgctgccg aactgcagcc ggtagaggcc gccgcccacc cgcacgtagg
    12481 gcgtcaattt tttttgcggg gctaagtcgg tcttgataaa gcccacgacg gggatgatgc
    12541 tcacgtccgg cgcggcggta ttcccgctca gctggtagag ctggtcaaag ccgtcggtgg
    12601 gatggtgcca attgagaccg gcggccaggc cgacctcgat ccggtcatgc acgtagtagg
    12661 aaaggctggc gctcccgccg aagccgctcc cgtaggcatc ggggaaatcg tcgggaacgt
    12721 tgggcgtatt cgaaaggccg atgggcagag ccggaccggt tccgaagccg accaccaccg
    12781 cccccttccc tttggcctcc tgtccgtgga ccgcaccagc cgctccgagg agggccaggg
    12841 tcgtcccgac caccacccgc ggccacactt ttgttttcgc cttggctctc atggctgctc
    12901 catcttctga attgtatcgg acattctctt ccgctgctcg actgccctgt tcccgctcat
    12961 ttgaaccaat cgaacgtgaa ccgggcgccg acccagacct gcacgtactg ccgtccggtg
    13021 aactcgttta tggtttcatc caggcggaag aggtcatgga ccaccgcttc cgtaaaaaaa
    13081 ctggtgcgtt cggtcaggtc gtaggagagg cccgcaccgc cgaatacccc aaaatcggtc
    13141 tccgtgtggt gagtggtgac ggtatcaatc cgggcccctt cctgatcctc aaaactgtta
    13201 ctctcgaaga ttacgttcat cgtccagccg cccttgaagt agggagagag ccgctgcgcc
    13261 gggcgcaggt gcaggctggc gaagatcccg aactgcgcga gggtcgtatc gggatcgggc
    13321 aggtcgcgca ggccgctggg caaaatgccg gtgggcctgg ccacccccgg gaaaaaattc
    13381 cccgacgccg tcaggccgaa gccgaccagg ttggaaaagt agtattcgac ctgcgccccg
    13441 ccggtaaaac ccatatccat ttgatcgttg aaatctccca ccggagtcaa tagccccaat
    13501 gccggcgcaa tagttacttt ccctttcaac tcggcaccgc cggctccggt ccagcccagc
    13561 ccgacgatac ctgccaaaag acaaaccacc accagtctac gcattgctcc tcctcgcgtt
    13621 gaattcctgt tcatcgcctg ccctttcccg tcagtgctcc ttttcgtgtg ccttggtctc
    13681 ctgcgcttct ttgatgattt tctccgcaat ctcccggggt acttcttcgt agtgcgaaaa
    13741 ttcccgccgg tgcaggccgc gcccctgggt gag
//
LOCUS       DBNGZA010000271         7206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1960432, whole genome shotgun sequence.
ACCESSION   DBNGZA010000271 DBNGZA010000000
VERSION     DBNGZA010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1960432"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="kdsA"
                     /locus_tag="AC16FU_05770"
     CDS             complement(<1..773)
                     /gene="kdsA"
                     /locus_tag="AC16FU_05770"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR2993914.1"
                     /translation="MSSTVKIGNFIVGRGNLLLIAGPCVIESETMVLRTAKRLKELAE
                     RCGFFLVFKSSYQKANRLSGKSYAGPGLDAGLKVLEKVKREFELPLTADIHSAGEAAS
                     VGEIVDLIQIPAFLCRQTDLVAAAARTGKAVNIKKGQFVAPEDMKSIAEKAIRAGNRN
                     ILLTERGTAFGYHNLVVDFRSLVIMRNLGYPVVYDATHSLQLPGANGATSGGQREFAL
                     PLARAAIACGVDGIFLETHPDPSRALSDRETQIPLAEMP"
     gene            complement(889..1341)
                     /locus_tag="AC16FU_05775"
     CDS             complement(889..1341)
                     /locus_tag="AC16FU_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993915.1"
                     /translation="MTANRTSVAFPNQGRKSAWLIVAAFAVAFLIPQCGGTPDSGGPI
                     KSRFPIPPIEEGLQIQAQLDEGHQPWRLDPKQVVIAYAAEIGIKNEVMSGDVLLAKDD
                     SMLSIVEVNSPKSQFRRRVYLARLLENPQNRFTIWFVVGLQNIPKKKA"
     gene            complement(1338..2999)
                     /locus_tag="AC16FU_05780"
     CDS             complement(1338..2999)
                     /locus_tag="AC16FU_05780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR2993916.1"
                     /translation="MKYIFVTGGVVSSLGKGIATASLGLLLKQRGLKVNLQKFDPYIN
                     VDPGTMNPFQHGEVFVLDDGAETDLDLGHYERFLDENLTRANNVTTGQVYEAVITRER
                     KGDYLGATVQVIPHVTNEIKERIKKVSHNEHDYDVVITEIGGTVGDIESQPFLEAIRQ
                     MGLEEPGENVLYIHLTLVPFIDTAGEVKTKPTQHSVKALREIGIQPDILVCRTARPLS
                     TELKEKIGLFCNVPSGAVIQAIDVDSIYEVPLLFHREGFDDLAVRHLQLNYPEPDLAP
                     WEKIVHKIKNPAYRIKIGICGKYVNLKDAYKSIIESFAHAGIENDAHVELEWISSENI
                     KREGAEKYLNNLDGLLIPGGFGDRGVEGKIEAIRYARESRLPFFGICLGMQCATIEYA
                     RNVCGLAQAHSSEFNPQSSQRVIDLLPEQHQVTQMGGTMRLGAYPCKLDRHSLAFKAY
                     GSEEISERHRHRYEFNNEYRKILAEAGFRITGTSPDGRLVEIIELSDHPWFVGVQFHP
                     ELKSRPTKAHPLYREFVRAALKHHLAQKESTPEPVPEAQVKAAVT"
     gene            complement(3013..3813)
                     /gene="kdsB"
                     /locus_tag="AC16FU_05785"
     CDS             complement(3013..3813)
                     /gene="kdsB"
                     /locus_tag="AC16FU_05785"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR2993917.1"
                     /translation="MARPAQKKETVWRESTFPKIVAVIPARYGASRLSGKPLVKINGK
                     PLVQHSYEQAKAFRLFADVIVATDDRRVAAAVRKFGGKVQMTDPDHPSGTERVAEVAR
                     KTACDIVVNLQGDELGLAPAYLERLIRPFIDRPVVQMTTLARPISSAREVRNPHQVKV
                     VTDRHGDALYFSRSPLPFPTKGASDHIAPLGHIGVYAYRRDFLLHLCTLPPTPLEKTE
                     RLEQLRVLEHGEKIHVAVVDTDTIRIDTPADLKRARQILERQGKKRNG"
     gene            complement(3818..4105)
                     /gene="gatC"
                     /locus_tag="AC16FU_05790"
     CDS             complement(3818..4105)
                     /gene="gatC"
                     /locus_tag="AC16FU_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR2993918.1"
                     /translation="MSITQKDVERIARLARLELSPAEKERYEKDLNTILSYMEQIHDV
                     DTSHVEIIDRVIELPPHLREDVVTPSLPVEAALANAPAREGNFFKVPKVIG"
     gene            4375..5313
                     /locus_tag="AC16FU_05795"
     CDS             4375..5313
                     /locus_tag="AC16FU_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR2993919.1"
                     /translation="MAKTEISAPARDTTYLHPATDFGIRFHYRTPKTVASRRLTIVML
                     LTFLMMVGEIIGGFLTHSLALLSDASHMLTHLAALGIALAAIKISQRPISTQKTYGYY
                     RFEILGAFVNGLTLFAIIAYILYEAVKKLITPQPIEAFAMFIVATAGLAVNLGSAFLL
                     RTSRKDDLNVQGAWFHLLSDTFSSVAIIIGGIVILKTNWYWIDPVLSVVIAVVIAIWG
                     IGLLRESTRVLLEATPSHVDIPAIAEAIREVPGVRAVHDIHVWALSSNMYAMTGHITV
                     DNIPVAETQEILANVNRMLEERFKIGHTNFQFEAIL"
     gene            5333..6370
                     /locus_tag="AC16FU_05800"
     CDS             5333..6370
                     /locus_tag="AC16FU_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HIR2993920.1"
                     /translation="MDDFEVFLKNLKDSLTAKQKQLLNLVYQNFKISAEWPKSFRIAV
                     DFRKNGDLYKIAEQLGMSLISVGQRYYHKDDESHLTLLGIYLCEGSEEDLRNFTEFAK
                     IAAKKFISNPENARISSSEAQELLNLEPLAFQRLSKLIYKGNLYFSSGGSWSPNGTWQ
                     FELTIEAIHYEDVQTIEDYLKAALYRKKSLIPKENPEIQLDLSSIKIEGIPPQLMKFF
                     QNLHPDIVNLCGKLVQNGHYSQAIFDAFKLVNKKVQEKSGKNDLDGASLMTTVFSVKK
                     PALQLNELKSKSDKDEQQGFMHIFQGAMIGIRNPKAHDIVEQKDPIKTLEYLALASLL
                     LRRIEESEPRS"
     gene            6381..6857
                     /locus_tag="AC16FU_05805"
     CDS             6381..6857
                     /locus_tag="AC16FU_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993921.1"
                     /translation="MNLAHLHLILNHIPVVGVPLTLLILLWGELRKDAAVIRLGWLLL
                     VVLAVITIPAFLTGEPAEEIVEHLPGVEKGLINTHEDHATLALIVTLAGGLFSLLALV
                     FSSTRPLLHRFLRVTILVAALFSTAALLWTANAGGKIRHTEIRSGAAAVPTEKEDE"
BASE COUNT         1559 a         2029 c         1969 g         1649 t
ORIGIN      
        1 ttcggcattt cggccaaagg aatttgcgtc tcccggtcgc tcaaggcccg ggaggggtcc
       61 gggtgcgttt ccaggaagat accatccacg ccgcaggcaa tggccgccct cgccaagggc
      121 agggcaaact ccctctggcc gccggaggtc gcgccattgg ccccgggcaa ttggagcgaa
      181 tgggtggcgt cgtacaccac cgggtatccc aagttgcgca tgattaccag cgaccggaaa
      241 tcgaccacca gattgtggta gccaaaagcg gttccccgct cggtcaagag aatgtttcga
      301 ttcccggccc tgatggcttt ttcggcgata gatttcatat cttccggcgc cacgaactgc
      361 ccttttttga tgttgaccgc tttccccgtg cgggcggcgg cggcgacgag atcggtctgc
      421 cggcagagga aggcggggat ttgaatgaga tcaacaattt ccccgacact tgcagcttca
      481 ccagccgaat gaatatcggc ggtcaaaggt aactcgaatt cacgcttgac cttctccaga
      541 actttcaaac cagcatccag tcccgggccg gcgtacgatt tcccgctcag gcggttggct
      601 ttttggtagg aggatttgaa caccagaaag aaaccacacc gctccgccag ctctttcaga
      661 cgcttggccg tccgcagtac catggtttcg gattcaatca cgcacggtcc ggcgatgagg
      721 agtaaatttc ctcggccaac gataaaattt ccaattttta cggtgcttga catcggttgt
      781 ctcacggata aaagaatata cgctgtccgt tccggccttc aaatcttttt gaccggctct
      841 gggacgagcc ggtctaccgg cttttgaatt gacttcaagc gctaagactt acgctttttt
      901 cttggggatg ttctgcaatc cgaccacgaa ccagatggtg aagcggttct gcggattttc
      961 caaaagccgc gccaggtaaa cccggcggcg gaactgcgac ttgggagaat taacctccac
     1021 aatcgacaac atgctatcgt ctttggccag aaggacgtcg cccgacatca cttcgttttt
     1081 gatcccgatc tccgccgcgt aggcaatgac gacctgcttg gggtcgagcc gccacggctg
     1141 gtgcccctcg tcaagttggg cttgaatttg caagccctct tcgatggggg ggatggggaa
     1201 gcgggatttg atcggccctc ccgaatcggg ggttcccccg cactggggaa tcaaaaaagc
     1261 aacggcgaat gccgccacta tcagccaagc tgatttcctt ccctgatttg ggaaggcgac
     1321 agacgttctg tttgctgtca cgttacggcc gctttcacct gtgcttccgg caccggttcg
     1381 ggcgtactct ctttttgcgc cagatggtgc ttcagcgcgg cccggacgaa ctcccggtag
     1441 agggggtgcg ctttggtcgg ccgggacttc aactccggat gaaactgcac tcctacgaac
     1501 cagggatggt cggacagttc gatgatttcc accagccgcc cgtccggaga ggtgccggtg
     1561 atgcggaagc cggcctctgc cagaatcttg cggtactcat tgttgaattc gtagcggtgg
     1621 cgatgccttt cggaaatttc ttcgctgccg taggccttga aggccagact gtggcgatcc
     1681 agtttgcagg ggtaggcacc cagacgcatg gtgccgccca tttgcgtcac ctggtgctgc
     1741 tccggcaaca ggtcgataac tcgctgcgaa ctttggggat tgaactccga actgtgggcc
     1801 tgagccagcc cgcagacgtt ccgggcgtat tcgatggtgg cgcactgcat ccccagacag
     1861 atgccgaaga agggcaggcg gctttcccgc gcgtagcgga tcgcttcgat tttcccttcc
     1921 actccgcgat cgccgaaacc gcccgggatc agaagcccgt ccaggttgtt cagatacttt
     1981 tcggcgccct cgcgtttaat gttctccgag gaaatccatt ccagttccac atgggcgtcg
     2041 ttttctatcc cggcatgggc aaacgactcg atgatgctct tgtaggcgtc cttgagattc
     2101 acgtacttgc cgcaaatccc gattttgatg cggtaagcgg ggtttttaat cttgtggacg
     2161 attttttccc agggagccag atccggctcc ggatagttaa gctgcaggtg ccggaccgcc
     2221 aggtcatcga acccctcccg gtggaacaag agcggcacct cgtagatcga atcgacgtca
     2281 atcgcctgga tgaccgcgcc ggacgggacg ttgcagaaca agccgatctt ctctttcagt
     2341 tcggtcgaaa ggggccgggc ggtgcggcag accagaatgt cgggctgaat cccaatttcg
     2401 cgcagggctt tgaccgaatg ctgggtcggc ttggtcttca cctcgccggc ggtgtcgatg
     2461 aagggcacca gcgtcaggtg aatgtagagg acgttctctc cgggttcctc taagcccatc
     2521 tggcggatcg cttccaggaa gggctgcgac tcgatgtcgc ccaccgtccc gccgatttcg
     2581 gtgatcacca cgtcgtaatc gtgctcgttg tggctcacct tcttgatccg ctctttgatt
     2641 tcgttggtaa cgtggggaat cacctgcacg gtggcgccca ggtaatcgcc tttgcgctcc
     2701 cgggtaatca ccgcctcgta gacctggccg gtggtgacgt tattggcgcg ggtcaggttc
     2761 tcgtccaaaa agcgctcgta gtggcccaaa tcgaggtcgg tctcggcgcc gtcatccagg
     2821 acaaacactt ccccgtgctg gaaggggttc atcgtcccgg gatcgacgtt gatgtagggg
     2881 tcgaacttct gcaggttgac tttcaatccc cgctgcttga gcaaaagtcc cagcgaagcg
     2941 gtggcgatcc ctttccccag cgaagaaacg accccgccgg tcacaaatat gtatttcacc
     3001 ggtcttttcg tttcagccat ttcttttttt gccctggcgc tccagaattt gccgcgcgcg
     3061 cttgaggtcg gctggcgtgt caatgcggat ggtatcggta tccacgacgg cgacgtgaat
     3121 cttctccccg tgctccaaga cgcgcaactg ttccaggcgc tcagtttttt ccaaaggcgt
     3181 gggcggcagg gtgcagaggt ggagcagaaa gtctcggcgg taagcgtaaa cgccaatgtg
     3241 tcctaacggg gcaatgtgat cgctcgctcc tttggtcgga aagggcaaag gcgaccgaga
     3301 gaagtagagc gcgtcgccgt gccggtcggt cacgaccttc acctgatgcg gattgcgcac
     3361 ctcgcgggcg gaggaaattg gccgggccag cgtggtcatc tgcaccaccg gccggtcgat
     3421 gaacggccgg atgagccgtt ccagataggc gggggccaag cccaactcat ccccctgcaa
     3481 attcaccacg atgtcacagg cagttttccg ggccacttcg gccacccgtt cggttccgga
     3541 cggatggtcg gggtcggtca tctgcacttt gccgccgaac ttgcgcaccg cggcggccac
     3601 ccgccggtcg tcggtggcga cgatcacgtc ggcaaaaagc cggaaggcct tggcctgttc
     3661 ataactgtgc tggaccagcg gtttgccgtt tatcttcacc agcggtttcc ccgagagccg
     3721 gctggcaccg tagcgggcag ggatgaccgc cacaatcttc gggaatgtcg attcccgcca
     3781 gactgtttct ttcttttgcg ccggacgcgc caccttttca cccgatcact ttgggcactt
     3841 tgaagaaatt cccctcgcgc gcgggtgcat tggccagggc cgcttccacc ggcagcgaag
     3901 gggtaaccac gtcctcgcgc agatgcggcg gcaattcgat cacccggtcg atgatctcca
     3961 cgtgggaggt atccacgtcg tgaatctgct ccatatatga cagaatggtg ttcaggtctt
     4021 tctcgtaacg ttctttttcg gctggggaaa gttccagacg cgccagccga gcaatgcgct
     4081 ctacgtcttt ttgggtgatg ctcatgtcgt ttcttccgga tacaaagagt gaatttcgct
     4141 tgattccaag atagcccggc ttttgacctt gtcaagaatc ttttcgtgcg gattccgtct
     4201 gaacgggtta agagtcgcgt agggcgaccc ctacgggccg tatggccgcc ctcggtcgcc
     4261 tacttgagcg cggcgagggc gccgcgccta cgcaatatcg ggagcaggga aatcccctat
     4321 gtaccgggcg ctctcttgac ggaaaagcgg gaaagaggtt gcttgaatag cgaaatggcc
     4381 aagacggaaa tctccgcccc cgcgcgggac accacctacc tccacccagc caccgacttc
     4441 gggatcagat ttcactaccg cacccccaag acggtggcat cccgacggtt gaccatcgtc
     4501 atgctcctga ccttcctcat gatggtgggg gaaatcatcg ggggcttttt gacccacagt
     4561 ctggcgcttt tgagcgacgc cagccacatg ctgacgcatt tagcggcttt aggcattgcg
     4621 ttagccgcca tcaaaatcag ccagcggccg atttccaccc agaagaccta cggctactac
     4681 cggttcgaaa tcttaggcgc tttcgtcaac gggctcaccc ttttcgccat catcgcttac
     4741 atcctctacg aggcggtcaa aaaactcatc accccgcagc cgattgaggc cttcgcaatg
     4801 ttcatcgtcg ccaccgccgg gctggcggtg aacttaggca gcgccttttt gctccgcacg
     4861 agcaggaagg acgacttaaa cgtgcaaggc gcctggttcc acttgctttc cgacaccttt
     4921 tcatcggtag ccatcatcat aggcggaatt gtcattctca aaaccaactg gtactggatt
     4981 gatccggtgc tttcggtggt gattgcggtt gtcattgcca tctgggggat cgggctactg
     5041 cgcgaatcga cgcgggtgct tttggaggcg acgcccagcc acgtggacat tcccgccatc
     5101 gcggaggcga tccgggaggt gcccggggtg cgggcggtgc acgacatcca cgtctgggcg
     5161 ctctcgtcca atatgtacgc catgaccggg cacatcaccg tagacaacat cccggtggcg
     5221 gagacgcagg agattttagc caatgtcaac cggatgttag aggagaggtt caaaatcggg
     5281 catacgaatt ttcagtttga agctatatta tagtgttgag gaaaaaacca ctatggatga
     5341 ttttgaagtc ttcctcaaaa acttaaaaga ttctcttacg gctaaacaaa aacagttgct
     5401 caaccttgtc taccagaact tcaaaatttc ggccgaatgg ccaaaatcgt tcagaatcgc
     5461 tgttgacttc cgaaaaaatg gggacctgta taagatcgct gaacagctgg gaatgtccct
     5521 gattagcgta ggtcaaagat actaccacaa agatgatgaa agtcatttga ctcttttggg
     5581 gatttatctg tgcgaaggtt cagaagaaga cttaaggaac tttactgaat ttgctaaaat
     5641 agccgcaaaa aaatttattt ccaatcccga aaatgctcga atctcctcct cagaagccca
     5701 agaattattg aacttagaac ccctcgcgtt ccaaagactt tcaaaattaa tttacaaggg
     5761 taatctatat ttttcttctg gtggaagttg gtcaccgaat ggtacctggc aattcgaact
     5821 tactattgaa gcaattcatt atgaggacgt gcagaccatc gaagattacc tcaaagcggc
     5881 tctctaccga aaaaagtcac ttatcccaaa agaaaacccg gaaattcaac tcgacctttc
     5941 ctctattaaa attgaaggaa ttccacccca acttatgaaa ttttttcaaa atctgcaccc
     6001 tgatatagta aacctttgtg gaaaacttgt ccaaaacggt cactattccc aagcaatctt
     6061 tgacgcattc aaattggtta acaaaaaagt ccaggaaaaa tcaggtaaaa atgacctaga
     6121 tggtgcttca ctaatgacca ccgtcttttc ggtaaaaaag cccgcattac aactcaatga
     6181 attgaaaagt aaatctgata aggacgaaca acagggattt atgcatattt tccaaggggc
     6241 catgatcggg atacgaaatc ccaaggcaca cgacattgtg gagcaaaaag acccgattaa
     6301 gacactggag tacttggctt tagccagttt gttgctacgc cgaatagagg aatcagaacc
     6361 gcgttcgtag gcaacagaga atgaacctcg cccacctgca cttaatctta aaccacatcc
     6421 cagtcgtcgg ggtgccgctc actcttttga ttttgctctg gggcgaactg cgcaaagatg
     6481 cggcggtcat ccggctgggg tggctgttgc ttgtggttct ggcggtcatt acgataccgg
     6541 cttttttgac aggtgagccc gctgaggaaa tcgtggagca cctgcccgga gtggaaaaag
     6601 gcctgatcaa tactcacgaa gaccacgcca ccctggcctt gatcgtaacc ctggccggcg
     6661 ggctgttctc gcttttggcg ctggtattca gctccacccg gccgctgctc caccggtttc
     6721 tccgcgtaac cattctcgtg gccgcccttt tctccaccgc cgccctgctc tggacggcca
     6781 acgccggcgg gaagattcgg catacggaga ttcggtcagg ggcggcggca gttccaactg
     6841 aaaaagaaga cgagtaaagt gtttcgggtg cgttaagatg accccaccct tccctcccct
     6901 acaggggagg gataacaggc gcgaggccta gagattcgct cgggggcggc ggctcccaca
     6961 tctgaaccag aggaagactg aagcaacccc tccctcaccc tcccctcaaa ggggagggac
     7021 atgtgcaggt gcgttacacc tacccttgac ccttcctccc caacgggata aattaccgca
     7081 aaaaggacag gttctctctc ttatttacag aaagggtaag gaggttctat gccccagacc
     7141 accgtgcaac ccaagaccgc cccggcggga gttgatttcc tgcctttgga ggggattgac
     7201 tacatc
//
LOCUS       DBNGZA010000272         5437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1850698, whole genome shotgun sequence.
ACCESSION   DBNGZA010000272 DBNGZA010000000
VERSION     DBNGZA010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1850698"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC16FU_05810"
     CDS             complement(<1..587)
                     /locus_tag="AC16FU_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993922.1"
                     /translation="MKQFLLLSILLTSRLTLAQTAPPANEIFLRQAVSDVWDSLLAGQ
                     LSQPFVWHLVSDSGHPGNWLVEAALIEKLQAQHLQVRSGISLAADSTATSQSVRRLGY
                     RIIDLSLAYPRIWRKGFTGRKELRRQARVALSARLSDPATGEILFDRRASVIREDQLT
                     SAQVREVENGNYPLLSPPVPPLGYNKYIEPIVVTGV"
     gene            complement(649..1305)
                     /locus_tag="AC16FU_05815"
     CDS             complement(649..1305)
                     /locus_tag="AC16FU_05815"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR2993923.1"
                     /translation="MSLPKRFGLCLRGGVVVSLLAVPHPAFGLSGSQIADSVKQRFTQ
                     AKTIFVQYRQETRQKGFATAGKITGKIWLAGEKKFRIENPEQTVVSDGESLWSYAPRL
                     KQVTIQKLSKVKNLVLPKDFLFSFFNDYSIATSGEETVAGKPCFRLTLKNLKPDMLIA
                     VMTVWVEQGSFFTRKVAYTDANDTRVTFSFPTIRTDVAVPGSRFTFAAPQGVEVVTLP
                     "
     gene            complement(1311..3623)
                     /locus_tag="AC16FU_05820"
     CDS             complement(1311..3623)
                     /locus_tag="AC16FU_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR2993924.1"
                     /translation="MEKERRKRTLTLRRLKLPRREVGGLLLFAVAVLMLLSLVTFDRL
                     RDRYALAQVDWTHPSSLWELRVPNQGGIVGAYVASGLYNAFGFLALFLPLYLGSWGAS
                     LTLKRSWPLFFKKTTLLLATFFFVACVAALSRGQAERLNETITLGGLFAQNAARFCTG
                     LFGVAGSATIFIAGALVLVTILFPLDWGKLSSHLRLPARPKLFWHTAAIRWGRAWEHL
                     KSRRQAEGEQSLSSPTRPQVVLREAAADPPIGAPPRPARVKKAEGPEELNLFSELQKT
                     LNIVKGPELPPEGDYRFPGVDLLNLPSVIKSAVSRDELEQTAQALKQTLETFNVQIEG
                     EAIEAYPGPVITRFEFKPAPGIKVNQIVSLADDLALAMQAKSIRIIAPIPGKAAVGVE
                     IPNRQPQTVYLREIISSGLFQRPERRLPLALGKTIGGEPFVADLSKMPHLLIAGATGS
                     GKSVCLNGIITSLLFRLHPDLLRFVFIDPKMLELSIYQGIPHLLRPVVTTPRQAEKVL
                     QEAVVEMEDRYRKLAQAAVRNIEDYNAKKKRADRLPYLLIVVDELADLMMSTNSARTV
                     MFVARLAQMARAVGIHLLLATQRPSVDVITGVIKANFSSRIAFQVASKGDSRVILDVN
                     GAEKLLGAGDMLFLQTGQPEPVRIHGAYISGQETEQIVNFLKSQTYQPVAAETPLVQT
                     SSPDLETDDTDEDADQLFHQAAELVIRHGQGSVSLLQRRLGIGYQRAARLIDQLEAAG
                     LVGPYVGSKTREVLVDKSYLERFTSPPAQS"
     gene            complement(3732..4004)
                     /locus_tag="AC16FU_05825"
     CDS             complement(3732..4004)
                     /locus_tag="AC16FU_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR2993925.1"
                     /translation="MTKAEVIARISKEAAISKAQAGRALDALFKGVTGSLKQGRRVSF
                     VGFGTFSVGKRKARMGRNPQTGATIKIAAARVPKFKAGKALKAAVK"
     gene            complement(4137..>5437)
                     /locus_tag="AC16FU_05830"
     CDS             complement(4137..>5437)
                     /locus_tag="AC16FU_05830"
                     /inference="COORDINATES: protein motif:HMM:NF040057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HIR2993926.1"
                     /translation="SLTKSFGSGVSVFAAGEFVRNLDTDARTLTLPTLSITKPQRPLF
                     SPKTEPGQIPIRRWYHSLYYSYGGSAINFSSRTPTASGKLVKHFATATQEVNLSTPQA
                     ILRYIAVTPNFNYRETWINVFPSNAADSAGVPTNSPARRGTYSLGIGALTNLYGTFPV
                     KIGGFQGLRHVVTPQISFAYTPKFTRNALLASYVGQPSTTARSEVMGFSLGNLFQIKT
                     RHKEEERKLEIANLNFSSGYNFTLTQRKLAPLVSSFRTTAVPAFGFEFNSVHDFYDSR
                     GNLHRPKLLNYSTTSTFSRGGTLGGGTLLADTAASAGPARAGTPTGIGYSLYLAHHYS
                     KAVGGGPAVNQLRWSLDLALSTRWSLRYTQSYDFTGRQVAAQQLNIHRDLHCWEADFS
                     WIPAGPSEGYYFVIRVKALPEIKLEKSRSGLGDILSNFVQ"
BASE COUNT         1130 a         1576 c         1655 g         1076 t
ORIGIN      
        1 acgccggtca ccacaatcgg ctcaatatac ttattatacc ccaagggggg caccggcgga
       61 gacaaaagtg gatagttccc gttctccacc tcccgcacct gggcactggt cagttgatct
      121 tcccggatca cgctcgcgcg ccggtcgaac agaatttcac cggtagccgg atcgctgagg
      181 cgggccgaaa gcgccacccg ggcctgccgg cgtaactcct ttcggccggt aaatcccttg
      241 cgccagattc tgggatacgc gagggacaga tcaattatcc gataccctaa ccgccgcacc
      301 gactgactgg ttgccgtact gtccgcagca agtgaaatgc ccgaccgcac ctgcaggtgc
      361 tgcgcctgga gcttttctat caacgccgcc tccaccagcc agttcccggg atggccggaa
      421 tcggaaacca ggtgccagac gaatggctgc gagagttgac cggccaaaag cgaatcccac
      481 acatcgctta ccgcttggcg caggaaaatt tcattggcag gtggtgccgt ttgggccaaa
      541 gtcaaacggg aagtcaaaag aatcgaaagg agcaggaact gcttcacgcg acccgctccc
      601 gtactgcaat ctcttcctct ccggaagtga taacgcgcct gatcatcgct aaggaagagt
      661 cactacttcc acgccttgcg gcgccgcaaa ggtgaaccgg gatccgggaa cggcaacatc
      721 ggtgcggatg gtcgggaacg agaaagtaac tcgagtatca ttggcgtccg tgtaggcgac
      781 tttccgcgta aagaaacttc cctgctccac ccaaacggtc atgacggcaa tgagcatgtc
      841 tggttttagg ttcttcaaag tcaatcggaa gcagggcttt ccggcaaccg tttcttcacc
      901 gctggtggca atgctgtagt cattgaaaaa cgaaaagaga aaatctttgg gcagaacgag
      961 atttttgact ttggacaact tttggatggt cacctgcttc agccgcggcg catacgacca
     1021 gagactttcc ccgtcggaaa ccaccgtctg ctccggattc tcgatgcgga acttcttttc
     1081 tcccgccagc cagattttgc cggtgatttt cccggccgtg gcaaacccct tctggcgggt
     1141 ctcctgccga tactggacaa agatggtttt ggcctgtgta aagcgctgtt tgaccgaatc
     1201 ggcgatctgg gaaccggaaa gtccgaaagc cgggtgtggt actgccagaa gcgagacgac
     1261 gacgccaccg cgcaggcaaa gaccgaatcg tttgggaagg gacatcggga ttagctctga
     1321 gccggtggac tggtgaagcg ctccaggtat gatttgtcga ccagcacctc gcgggtcttg
     1381 ctgccgacgt acggccccac gagccccgcg gcttcgagct gatcaatgag ccgcgccgcc
     1441 cgttggtacc cgatccccaa ccgccgctgc aaaagcgaga ccgagccctg cccgtgccgg
     1501 atgacgagct ccgccgcctg gtggaacagt tgatcggcgt cctcgtctgt gtcgtcggtc
     1561 tccagatcgg gtgaggacgt ctgcaccaaa ggcgtttcgg cagccaccgg ctgataggtc
     1621 tggctcttga ggaagttgac gatctgttcg gtttcctggc cggaaatgta ggcgccgtgg
     1681 atgcggaccg gctccggctg gccggtctgc agaaagagca tatcccccgc acccaaaagt
     1741 ttttcggcgc cgttcacatc taagatgacg cgcgaatcgc ctttggaagc cacctggaag
     1801 gcgatccggg aggaaaagtt ggctttgatc acgcccgtga tgacgtcgac cgacggccgc
     1861 tgcgtggcta aaagcaagtg aatccccacc gcccgcgcca tctgcgccag acgggcgacg
     1921 aacatcaccg tccgcgccga attggtgctc atcatcaggt cggccagttc gtccacgacg
     1981 atcaaaaggt acgggaggcg gtcggcccgt ttcttcttgg cattgtagtc ctcgatgttc
     2041 cgcacggcgg cctgcgctaa tttccggtag cgatcctcca tttcaaccac tgcttcctgc
     2101 aacaccttct ccgcctgacg cggggtggtg actaccgggc gcaataggtg cgggatgccc
     2161 tggtagatag agagctctaa catcttggga tcgatgaaaa cgaagcgcaa aagatccggg
     2221 tgcaggcgga agaggagact ggtgatgatg ccgttcaaac agacgctttt gcccgagccg
     2281 gtggctccgg cgatgagcag gtggggcatc ttggagagat cggccacgaa cggctcgccc
     2341 ccgatcgttt tccccaaggc caggggcagc cgccgctccg gtctttgaaa caatcccgaa
     2401 gaaatgattt cgcgcaggta gaccgtctgc ggctggcggt tggggatttc aacccccacc
     2461 gccgcttttc ccggaatcgg cgcgatgatc cggatgctct tggcctgcat ggccagagct
     2521 aaatcgtcgg ccaaactgac aatctggttg accttgattc cgggggccgg cttgaactca
     2581 aagcgggtga tgaccggccc gggataggct tcgatcgctt cgccttcaat ctgcacattg
     2641 aaggtttcga gcgtttgctt gagcgcctgg gccgtttgct ctaattcatc gcgggagacc
     2701 gcgcttttga tgaccgaagg gaggttcaaa agatcgactc cgggaaaacg gtagtcgcct
     2761 tccggcggga gttcgggccc tttgacgatg ttaagggttt tctgcaattc ggaaaacaaa
     2821 ttgagttctt cgggcccttc ggcttttttc acccgcgccg gacgcggtgg cgccccgatc
     2881 ggcgggtcag cggctgcctc gcggagcaca acctgcggtc gcgttgggga tgaaagcgac
     2941 tgctccccct ccgcctgccg ccgggatttc aaatgttccc acgcgcgtcc ccagcgaatt
     3001 gcggcggtgt gccagaagag cttcggtcgg gccggcaggc gaaggtgact ggagagtttt
     3061 ccccagtcga gggggaaaag aatcgtcacc aggaccagcg ctccggcgat aaaaatcgtg
     3121 gccgacccgg ctaccccaaa aagaccggtg cagaaacgcg ccgcgttctg ggcgaaaagg
     3181 ccgcctaaag tgatggtttc attcaaccgc tccgcctgcc cgcgcgacag cgccgccacg
     3241 caggcgacga agaagaacgt cgccaaaagg agagtcgttt tcttgaaaaa gagcggccag
     3301 gagcgtttga gcgtcagact ggcgccccag gaaccgaggt agagcggcag gaagagcgcc
     3361 agaaagccga aggcattgta aagcccggac gccacgtagg caccgacaat tcctccctgg
     3421 ttcggcaccc ggagttccca gagactggag ggatgcgtcc aatcgacctg ggccagggcg
     3481 tagcggtcac gcagccggtc gaaagtgacg agactcaaga gcatcaaaac cgccaccgca
     3541 aaaaggagta ggccgccgac ttcgcggcgc ggtaacttca gccggcggag agtgagagtc
     3601 cttttccttc tttccttctc catggataag gagaatctaa catcggcgtg cgcccggcgt
     3661 caataaaaaa gccccgccgt ggcggggctt cacactgtca gcagagtgaa agctctgccg
     3721 ccatgggaga attactttac cgcggccttg agggcttttc cggctttgaa ctttggcacc
     3781 cgcgccgcag cgatcttgat ggtggcgccg gtctgcgggt tgcggcccat acgcgctttc
     3841 cgctttccca ctgagaaagt gccgaaaccg acgaacgaga cccgcctacc ctgtttgagc
     3901 gaaccggtca cacctttgaa gagggcgtcc agtgcccggc cggcctgcgc cttggaaatg
     3961 gcggcttcct tggagatcct ggcaatcact tccgctttgg tcatgctctc acccccttag
     4021 atcggcaaag ggtttcacgc tccatactcc gcgacctgtg cggcgagaat agcgttcggg
     4081 tgctggtttg tcaagacatt tttgggggaa ttgcgcaatt ttttccgctc ccccggttac
     4141 tggacgaagt tggagaggat gtcgcccagc ccggagcgcg atttctccaa tttgatttcg
     4201 ggcagggcct tcacccggat cacaaagtag tacccttcac tcggccccgc ggggatccag
     4261 gagaaatcgg cctcccagca gtgcaggtca cggtggatat tgagctgctg cgccgctacc
     4321 tgccggccgg tgaagtcgta gctctgcgtg tagcggagac tccaacgggt ggagagggcg
     4381 aggtccaagg accagcgcaa ctggttgact gcggggccgc cgcccaccgc cttggagtaa
     4441 tggtgcgcca ggtagaggct ataaccgata ccggttggcg ttcccgctct ggccggtccg
     4501 gctgatgcag cagtatccgc aagcaaagtt ccaccgccca gagtgccacc ccgggagaag
     4561 gtggacgtgg tggaataatt caaaagttta ggtcggtgga gattgccccg gctgtcatag
     4621 aaatcgtgca ccgagttgaa ctcaaatcca aaagccggca ccgcggtggt gcggaaactg
     4681 gaaaccaaag gcgccagttt ccgctgcgtg agcgtgaagt tgtaaccgga ggaaaaatta
     4741 agattggcga tttccaactt ccgctcctct tctttgtgcc gggttttgat ttgaaacaga
     4801 ttccccaggg aaaaacccat gacctcgctt ctggcggtcg tggagggctg gccgacgtaa
     4861 ctggccaaga gggcgttcct ggtgaacttg ggtgtatagg cgaaactaat ctgaggcgtc
     4921 accacgtggc ggagcccctg aaacccgccg atcttcaccg gaaaggtgcc gtaaaggttg
     4981 gtcagtgctc cgatcccgag actgtaggtt ccccgccgag ccgggctgtt cgtcggcacc
     5041 cccgcagagt cggccgcgtt ggaggggaac acattgatcc aggtttcccg gtagttgaag
     5101 ttcggcgtca ccgcgatata gcgtaaaatc gcctgcggag ttgacagatt cacctcctgg
     5161 gtggccgtgg cgaagtgctt gaccagtttc cctgaggccg tgggcgtccg actggagaaa
     5221 ttaatggcgg aaccgccgta ggagtagtag agcgaatggt accagcgccg aatcggaatt
     5281 tgtcccggct cggttttcgg gctgaagagc ggccgctggg gcttggtgat cgaaagggtg
     5341 ggcaacgtca acgtccgcgc atcggtgtcc aggttgcgga cgaactcgcc ggcggcaaag
     5401 acgctcacgc cgctcccgaa cgacttcgtc agactgg
//
LOCUS       DBNGZA010000273         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2063383, whole genome shotgun sequence.
ACCESSION   DBNGZA010000273 DBNGZA010000000
VERSION     DBNGZA010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2063383"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16FU_05835"
     CDS             complement(<1..466)
                     /locus_tag="AC16FU_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR2993927.1"
                     /translation="MILRTDFLVIGSGIAGLGWALKMAPHGQVVVVTKKQDTESNTNY
                     AQGGIAAAVGSDDSPELHFQDTLAAGAGLCHPEVVRRVVEVAPQLIRELLDLGVPFSF
                     RGGGGSLSDLALFREGGHSRARVVHAADATGKAIERVLAGRVKSQRNITVLEN"
     gene            complement(463..1527)
                     /gene="lpxK"
                     /locus_tag="AC16FU_05840"
     CDS             complement(463..1527)
                     /gene="lpxK"
                     /locus_tag="AC16FU_05840"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR2993928.1"
                     /translation="MTANLLQRYRFLFSPLLSATAALFRVGVGLRHWSYRWGVVRSVP
                     LKTKVVSIGNISAGGSGKTPLVAYLARRLAGEGKRVCVVTRGYRGKARALVALLPGER
                     PARDWMEIGDEARWLQKELPAVPLVIHSKKYQAARWADQNLNPDVLLIDDGFQHQRLT
                     RDFDLVMVTPDDLDPNNHLLPRGRLRERPQALARAGFIFLNATGGEDESLDLLDRIRR
                     YAPETPLGWGYYTASEIVEVPSQKKLPVTALDGAPCLAFSGIGRPHGFAAVLARHRFH
                     VVAEERFFDHYPYRKIDIQTLVAEARRVRAEFCVTTEKDAVRLAGLWTGELPLYAVRA
                     KIVFAEGEERLWQLLKRLWA"
     gene            complement(1540..>2233)
                     /locus_tag="AC16FU_05845"
     CDS             complement(1540..>2233)
                     /locus_tag="AC16FU_05845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002813058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR2993929.1"
                     /translation="PAERVEVVGSLKFDHQIKAGLPRLRQEVRNRLHLAPDRPVVVAG
                     STRPGEEELVIEAFASLRQKFPQALLIIAPRHLKRLGAVEILLQQKQLAAQRLGVYRQ
                     NGSGAEDLSVLVVDSMGELALLYAAGDVAFVGGSLVDYGGHNPLEPAVVGTPVVFGPF
                     TDHAQRAAEILLASGLGTEVRSVAELVLAIEKQLLSRAQPPDGFARFQTLLERESGTA
                     ALCVEKLRPYLS"
BASE COUNT          452 a          725 c          652 g          404 t
ORIGIN      
        1 gattttccaa gacggtgatg ttgcgctggg atttcacccg gcccgccagg acccgctcga
       61 tcgccttacc ggtggcgtca gccgcgtgga ccacccgcgc ccggctgtgt ccgccttcgc
      121 ggaataaggc gagatcggaa agagagccgc ccccgccgcg gaaagaaaaa ggaacgccca
      181 gatctaagag ctcgcggatc aactgcggag ctacttccac cacccggcgc acgacttccg
      241 ggtggcatag tcctgctccg gctgccaggg tatcctgaaa gtggagttcc ggagaatcgt
      301 cggaacctac cgctgccgca atgcccccct gggcgtagtt ggtgttcgac tcggtgtctt
      361 gcttcttcgt cacgaccacc acctggccgt ggggagccat cttgagcgcc cacccgagcc
      421 cggcaatgcc ggaaccgatg accagaaagt cggtacggag gatcatgccc aaagcctttt
      481 gagcaattgc cagaggcgct cttctccctc ggcgaaaacg attttggccc gcaccgcgta
      541 gagcggcaat tcaccggtcc agagcccggc cagccgaacc gcatctttct cggtggtcac
      601 acagaactcc gcccgcaccc ggcgcgcctc tgcgaccaac gtctgaatgt caatttttcg
      661 atacgggtag tgatcaaaga accgctcttc cgccacgacg tggaaccggt gccgggccaa
      721 aaccgccgca aatccgtgcg gccgcccgat cccggaaaaa gcgagacagg gtgcgccgtc
      781 gagtgcggtc accggcagct ttttctgcga cggtacctca acgatttcgg aagcggtata
      841 gtatccccac ccgaggggtg tctctggggc gtacctgcga atacggtcga gcaaatctaa
      901 ggattcatct tcgcccccgg tggcgttgag gaaaatgaaa ccagcccgcg ctaatgcctg
      961 cggccgctcg cgcagccggc ctcgcggcaa caggtgattg ttcggatcca gatcgtccgg
     1021 cgtgaccatg accaagtcaa aatctcgggt gagtcgctgg tgttgaaagc cgtcatcgat
     1081 cagtagcacg tcggggttga gattttggtc agcccagcgc gccgcctgat acttttttga
     1141 gtggataacg agcggcacag cgggaagttc tttttgcaac cagcgcgctt catccccgat
     1201 ttccatccaa tcccgtgccg gccgttcacc cggcaggagc gccaccagtg cccgcgcctt
     1261 cccccgatac ccgcgcgtca ccacacagac tcgtttgccc tccccggcca gtcgccgggc
     1321 aaggtaggcg accagaggag ttttcccgct cccgccggca ctgatgttcc cgatactgac
     1381 gactttcgtc ttcagcggga ctgaccgcac caccccccag cggtacgacc aatggcgcaa
     1441 accaacaccc actcgaaaaa gagccgcggt cgcgctgagc aatggagaaa aaagaaaccg
     1501 gtaacgttgg aggagattcg cggtcatggc gatcaacgct caggaaaggt acggccgcaa
     1561 cttctccaca cagagagcgg cggtgccgct ttcccgctcc aggagagttt ggaaccgggc
     1621 gaaaccgtcg ggcggctggg cgcgggagag caattgcttt tcgatggcta ggacaagttc
     1681 agcgaccgag cgcacttcgg tgcccagccc ggaggccaaa aggatttccg ccgcccgctg
     1741 ggcgtgatcg gtaaaaggcc cgaagacaac cggagtcccc accaccgctg gctccaaggg
     1801 attatggccg ccgtaatcga cgagactccc tccgacgaaa gccacgtcac cggcggcata
     1861 gagcagagcc aattccccca tgctgtctac gaccaaaacg gagagatctt ccgctccgct
     1921 gccgttttgt cggtagacac cgagcctttg ggcagccaac tgtttttgct gcaagagaat
     1981 ttctacagcc cccagacgct tgagatgccg gggcgcgatg atcaaaagtg cttgggggaa
     2041 cttttgtcgg aggctggcga aggcctcgat caccagttcc tcttcgcccg gccgggtact
     2101 tccggccacc accaccggac gatccggggc cagatgcagt cggtttcgga cttcctgtct
     2161 caggcggggc agcccggctt tgatctggtg gtcgaacttg agactgccca ccacctccac
     2221 ccgctctgcc ggt
//
LOCUS       DBNGZA010000274         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1532949, whole genome shotgun sequence.
ACCESSION   DBNGZA010000274 DBNGZA010000000
VERSION     DBNGZA010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1532949"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16FU_05850"
     CDS             complement(<1..388)
                     /locus_tag="AC16FU_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993930.1"
                     /translation="MKVAHDPAKLLEKYLILKDFSINEERAISERARNYLTTTAILFT
                     AYGLALQIAFLRYASPIIGILYNFFWYYLGKRNALAFYYWRREMHLIEEEFQKIGQGY
                     EVYNIFSNRDRYYAGEPVATLSSDLVE"
     gene            complement(385..1629)
                     /locus_tag="AC16FU_05855"
     CDS             complement(385..1629)
                     /locus_tag="AC16FU_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR2993931.1"
                     /translation="MPAEIVTIGDELLSGQTVDTNAAFIGEKLMAAGIPVRWRTTVGD
                     AEADIAEALERAHKRAEVVIATGGLGPTHDDVTKKAIVQVFRRSLVFHDDVLKKVQAL
                     FSRRGLEMPKINQNQALLPQGTKIIDNPVGTAVGIWLEEDGRVFVALPGVPAEMRPML
                     LEGVLPLLRQEGRRFLLQRKLRTTGIPESALYERLAAKIAGEGEIRVAFLPGFLGVDI
                     RLSLLTANREHGQRQLETFVEALRPLIGPVLYGVDAETLEKVVGQLLAERKLTLAVAE
                     SCTGGMLGERITNISGSSKYFDRGVVTYSNQAKMDLLGVPAILIEKHGAVSAEVAGAM
                     AEGVRRAARTDFGLSITGIAGPAGGTAEKPVGLIYIGLAASSQTITRRFIFGTERDLN
                     RQRASQAALDLLRLYLLNEETA"
BASE COUNT          416 a          580 c          547 g          423 t
ORIGIN      
        1 tctccaccag atcggaagag agggtagcca ccggctctcc ggcatagtag cggtcgcggt
       61 ttgaaaatat gttgtagacc tcgtacccct ggccgatttt ctggaactcc tcctcaatca
      121 aatgcatctc gcgccgccag tagtagaaag ccagggcgtt acgcttgccc agatagtacc
      181 agaagaagtt atagagaatc ccgatgatcg gactggcgta gcggaggaag gcgatttgca
      241 gcgccagacc gtaggcggta aaaaggatcg ccgtggtggt caggtagttg cgcgcacgtt
      301 ccgaaatcgc ccgctcttcg ttaatggaaa agtctttcag aatcaggtat ttctctaaaa
      361 gcttggccgg atcgtgagcg actttcatgc cgtttcctcg ttgagaaggt agagccggag
      421 caagtcgaga gccgcttggc tggcccgctg gcggttcaag tcccgttcgg tgccgaaaat
      481 gaaacgccgg gtaatggtct ggctgctggc cgcgagaccg atgtagataa gcccgaccgg
      541 cttttccgca gtgccgcctg cggggccggc aatgccggtt atagaaagac cgaaatcggt
      601 tcgagccgcc cggcgcacgc cctccgccat cgctccggcg acttccgcgc tgaccgcccc
      661 gtgcttttcg ataagtatgg cgggaacgcc taaaaggtcc attttcgcct gattgctgta
      721 ggttaccacc ccacggtcaa aatatttcga actacccgaa atgttagtga ttcgctctcc
      781 cagcatcccg ccggtgcacg actcggccac ggctaaagtc aatttccgct cggccagcag
      841 ttgtcccaca actttctcta aggtctcagc gtcaaccccg taaaggaccg ggccgattaa
      901 gggcctgagc gcttcaacga acgtttccaa ctggcgctgg ccgtgctctc gatttgcagt
      961 caaaagtgaa aggcggatat ccaccccgag aaaaccgggc aagaaagcca cgcggatttc
     1021 gccctccccg gcgattttgg ccgccagccg ttcgtaaagc gccgattcgg gaataccggt
     1081 cgtccgtaat ttgcgctgga ggagaaaccg ccggccctct tggcgcaaaa gcggcagtac
     1141 gccttccaaa agcatcggcc gcatttccgc cggcacgccg ggcagcgcca caaagacccg
     1201 gccgtcttct tccagccaga tccccaccgc ggtgccgacc gggttgtcga tgatcttcgt
     1261 cccctgcggt agcagggctt gattctggtt gattttgggc atttccaaac cgcgccggga
     1321 gaacagcgcc tggacttttt tcaaaacgtc gtcgtggaag actaagctcc gcctgaaaac
     1381 ctgcacaatg gctttcttgg tgacgtcgtc gtgcgtgggg cccaggccac cggtggcaat
     1441 caccacttcc gcccttttgt gggcacgttc caatgcctcg gcaatatcgg cctcggcgtc
     1501 gcccacggtg gtccgccagc gcaccggaat gcccgccgcc atcaattttt caccgataaa
     1561 agcggcattc gtatcgacgg tttgtcccga caagagttca tcgccaatgg tgacaatttc
     1621 agcaggcata ggaacttgcg tgggtaaccc atcctcttcc gaagaatgct ttcaacaaag
     1681 gtcttcgtcc tacccgaaga gaaatatgcc cgaatcactt tcaaagtcaa ggcagggaaa
     1741 ctttgccctc gtttggccgg ttcactatat tggtgcgccg tttcgatttg aactgaaaga
     1801 aacccaaacg tgaggtcgcc gatgcgcaga gggacaggaa tcgtaggtac ggggattttt
     1861 tgtttcttcg tcagtacgtc actccttgag ggggcaatgg tggaaccggc cttacaggcc
     1921 cggctggccc gggccaatcc cgccgatacc ttccgtgtgc tcctgg
//
LOCUS       DBNGZA010000275         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2487692, whole genome shotgun sequence.
ACCESSION   DBNGZA010000275 DBNGZA010000000
VERSION     DBNGZA010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2487692"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="AC16FU_05860"
     CDS             <1..982
                     /locus_tag="AC16FU_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993932.1"
                     /translation="RTFITQKGVAVPEDGWDADVKENPKDAKIESKTYPAERWLEVLS
                     PLSSAVAKFPSDQNRTLGTAALPVDGILTMETVEALNKAGMSSINWSEIDAADWKDVR
                     QKETEFKTGPDQEDNDSVRQRIVYLAESQIGHVLASSRGDDKKFGWQRIIRYYEVALG
                     KEWAEKRFGKITDPSQASQQPQEKETEAHERVQKSGIFAFTRPGPWSWCGIFAMWAVK
                     SITGRGNWQEGPKELTEVRRSTDPKLRGAKRGDILSITSSNSHFCLLATDVPEVADDS
                     TVLYTIDGNVEDQAIRANFKWKIANVGSYFRAVDDAATAAAPSKPKATDK"
     gene            complement(1322..1630)
                     /locus_tag="AC16FU_05865"
     CDS             complement(1322..1630)
                     /locus_tag="AC16FU_05865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR2993933.1"
                     /translation="MTPHRTHPTRADFRFWWPMTVRWGDMDAMGHVNNIIYFQYLESA
                     RMGFAEAIGWGIRTGSGGRQGPAVVSQTYHSRRQVFYPAELEVGIRCKEIRGRSFVLE
                     "
BASE COUNT          454 a          526 c          529 g          376 t
ORIGIN      
        1 acgcaccttc atcacacaaa agggagtggc agttcctgaa gatggctggg atgctgatgt
       61 gaaagagaac ccgaaagatg caaaaattga atcgaagacc tacccggcgg aacgctggct
      121 cgaagtactt tcgccgcttt ccagcgccgt agcaaaattc ccctcggacc agaatcgcac
      181 actcggcact gctgcattac cggtcgatgg catattaacg atggaaacag tcgaggcact
      241 aaataaagcc ggaatgtcca gcattaattg gtcagaaata gatgcggctg actggaagga
      301 tgtccgccaa aaggaaacag aattcaaaac cggcccagac caggaggata atgattctgt
      361 acgccaaagg attgtatatc tcgcggagag tcaaattggg catgttttgg cttcaagccg
      421 aggtgatgac aaaaaattcg gctggcaacg catcattcgg tattacgaag tggccttagg
      481 gaaggaatgg gctgagaaga gatttggaaa aatcacagac ccctcgcaag ccagccaaca
      541 accccaagaa aaagaaaccg aggcgcatga gcgcgttcag aaaagcggca tttttgcctt
      601 caccagaccg ggtccctggt catggtgcgg catatttgcc atgtgggctg tcaaatccat
      661 taccggaaga ggaaactggc aggagggccc caaggagctg accgaagttc gacgcagcac
      721 cgatccaaaa ttgcggggag ccaaacgggg cgatatactt tccattacca gcagtaacag
      781 ccatttctgc ctgttagcca cggatgttcc agaagtggcg gatgactcaa ccgtgttata
      841 caccattgac ggtaacgttg aggatcaggc tattcgcgcg aatttcaagt ggaagatcgc
      901 aaatgtcggc tcctatttcc gcgctgtaga tgatgcggcg actgcagccg ccccgagcaa
      961 gcccaaggct acagacaaat gaacatccct ttttagtggg caagagtgga acgcgtcttg
     1021 aaggtggaac gactcactgc gcaaagcgaa aaacttagtg gagcacctgg gggcgtaggc
     1081 ccgcctgctg gggaagccgg cgccgttttg cgtaggttga caagagcgtt ccatcgttcg
     1141 gcagttcgga gtacggctga atcggccaaa ggggggaaca gccgaacatt gttcagttct
     1201 cggtcagggc ccggcgcagg tccggcggga tgtccagcgc cttgcctcca tcgtagtcca
     1261 gccacaccac cacgctgctc ccgtcgccca ccagctcgtc cgtccccttt cgataaatgc
     1321 cctactccag gacaaaactc cgaccccgga tctctttgca tcgaatcccc acctccagct
     1381 cggccgggta gaagacctgg cgacgagagt ggtaggtctg ggagacgacc gccggcccct
     1441 gccggcctcc gctcccggtc cgtatgcccc acccgatcgc ttcggcaaag cccatgcgcg
     1501 cagattccag gtactggaag tagatgatgt tgttcacgtg gcccatggcg tccatgtcgc
     1561 cccagcggac ggtcatgggc caccagaacc ggaaatcggc gcgggtcggg tgagtccgat
     1621 ggggagtcat ggggagtgat tctctctgca cggtcagccc tcagccatca gcttttgcgt
     1681 gccgtgacct gccgagatgt caccgtccct ttgccggttt gatcttgcgg agcttcggga
     1741 cgtgcttcag gatggatcgg cgatcccgct caaaagaact ccgcggaacc tttcggccaa
     1801 ccgattgtga tcttcgaccc acggggcgtt ccctcctgga ccaatctacc gttactatca
     1861 tgtcgttttc aagccctttt ccaga
//
LOCUS       DBNGZA010000276         6265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2593430, whole genome shotgun sequence.
ACCESSION   DBNGZA010000276 DBNGZA010000000
VERSION     DBNGZA010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2593430"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..259)
                     /locus_tag="AC16FU_05870"
     CDS             complement(119..259)
                     /locus_tag="AC16FU_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993934.1"
                     /translation="MTPGIKYAPTDAVGLAGGVQIPIHQDLFGIQDEDPVFIVGLAYD
                     WR"
     gene            complement(312..446)
                     /locus_tag="AC16FU_05875"
     CDS             complement(312..446)
                     /locus_tag="AC16FU_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993935.1"
                     /translation="MRKVVSTFSVFSVLCGFWVVFYQQAQACSVCLAGGCGDKRALCR
                     "
     gene            complement(555..2510)
                     /locus_tag="AC16FU_05880"
     CDS             complement(555..2510)
                     /locus_tag="AC16FU_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2993936.1"
                     /translation="MAGVQAQPFLRSVTSFPINDSAGPVAMPFLGGIDSPKPQLVDLD
                     RDGDFDLVILQKDGRLVFFRNTGTLSSPQFDYGADSFLGINAGQWFRLADIDGDGDPD
                     LFVDNNTGGMRFYRNDSPPDSFIFNKISQNFQNIFSGFNNTPAFADIDGDGDFDFFLG
                     ELTGTLTYWRNSGTPLSPNFVFGANDFDSIVAFPTVGCSVSVAPPPPGQLAEAQAHGI
                     NGIQFADLDGDGDFDFFWGDIFNTNMYFFRNQGTPLNSHLQAITCTFLPVSTIGFNEP
                     AFADLDADGDPDLVVGSANFGSSIDNLLYFRNVGSPTAPRESLITRNLLSNLDFGANS
                     YPAVVDWDGDGLPDLFCGRDDGRMAAYRNVGSQRHPAWQFVSSGFGGVDVGASSTPAL
                     GDLDGDGDLDMICGNEDGRLALFRNTGTPSNPVFTLTDGFFLGLDLDFNASPVLVDID
                     HDGDLDLFVGEWDNQANANLYFYRNEGGPFPPNFVQEATALLPPTGLQQTLPAFADID
                     GDGDYDLFLGTSDGTIWFYRNSGDSTTFNFVKETENFENIDVGQTAAPFLVDIDYDGD
                     PDLVAGESDGGLNLYCNQALAVGDVTRDGVVDISDLVSLIQRVVFGALLATPDATGDL
                     NCDGVLDIIDIVALVQNVVFGAPPPCI"
     gene            2986..3213
                     /locus_tag="AC16FU_05885"
     CDS             2986..3213
                     /locus_tag="AC16FU_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2993937.1"
                     /translation="MARGKVKWFNEKKGFGFIQQDGGGQDIFVHYTAIKAEGFKSLVE
                     GDEVEFTVQEGPKGPQATNVSRVVAEGNRAL"
     gene            complement(3282..3557)
                     /locus_tag="AC16FU_05890"
     CDS             complement(3282..3557)
                     /locus_tag="AC16FU_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993938.1"
                     /translation="MMQNWLKIFGGFFWFGLGLIVLGKHATQYPEIRNFAGGGPLVAC
                     YVLTIFGTIFFVIGLYGVLSILRRFATENKLALRFFNLILPFGKFGL"
     gene            complement(3790..4153)
                     /gene="ssrA"
                     /locus_tag="AC16FU_05895"
     tmRNA           complement(3790..4153)
                     /gene="ssrA"
                     /locus_tag="AC16FU_05895"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4271..4343)
                     /locus_tag="AC16FU_05900"
     tRNA            complement(4271..4343)
                     /locus_tag="AC16FU_05900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4308..4310),aa:Lys,seq:ctt)
     gene            complement(4392..4463)
                     /locus_tag="AC16FU_05905"
     tRNA            complement(4392..4463)
                     /locus_tag="AC16FU_05905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4429..4431),aa:Lys,seq:ttt)
     gene            complement(4511..5791)
                     /locus_tag="AC16FU_05910"
     CDS             complement(4511..5791)
                     /locus_tag="AC16FU_05910"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR2993939.1"
                     /translation="MPNRIVVGMQWGDEGKGKVVDLLSREADIIARYQGGANAGHTVV
                     IGSEKFILHLIPSGILHPGKICVIGNGVVVDLEQLVTEIDELEKRQIGVSGRLFISGA
                     AHLVLPYHKALEQSQESHQREKIGTTGRGIGPAYTDKFARLGIRVIDLWDEKRLAAKF
                     TENLAWKRPLIEATNGKFDWNEKKLVDLACGYRDRIKPLVTDTSLYLHNAIKAGKNVL
                     FEGAQGTLLDVDFGTYPFATSSNTIAGGAMTGLGVGPRCVDEIIGVMKAYTTRVGNGP
                     FPTELTDAVGQKLQKRGNEFGATTGRPRRCGWLDILALKYAVRVNGITKIALMKLDVL
                     DGLPELKICIAYKFHGEQLKELPVDLAALGDCEPVYETYPGWSGVTSGVTVYGKLPEP
                     ARRYVEKIQKLLGVPMLLVSTSDRREETIWLQAS"
     gene            complement(5784..>6265)
                     /gene="yihA"
                     /locus_tag="AC16FU_05915"
     CDS             complement(5784..>6265)
                     /gene="yihA"
                     /locus_tag="AC16FU_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR2993940.1"
                     /translation="LNFFLVNEQIWFTDLPGYGYAKVEKKLREQWGTLVEKYLQETRD
                     LARVIQIVDSRHPPFPDDLELAEWLSHIGLRFLVVLTKADKLSRGEEAQALKRAQEAF
                     SPSAPEKITLFSATSGRGRDEVLRWIGQVIRDYRDAEREVRHSRAGRGDEEMTEHHA"
BASE COUNT         1485 a         1805 c         1643 g         1332 t
ORIGIN      
        1 ggagcgaacc ggccagaaag ccaacgacaa cagaaatcat ctttttaatc tttcaaaaag
       61 gacactttca tagggccaag gttactgccg acagttgggc gggcaaactt ctgaaatctt
      121 accgccagtc gtacgccagt cccacgatga aaaccgggtc ttcgtcctga ataccgaaga
      181 gatcctggtg aatcggaatc tgcaccccac cggccagtcc cacggcgtcc gtgggcgcat
      241 atttgatccc cggcgtcacg taggtgaccc acccacccgt atgaggctca accagaccgg
      301 atatttcgtc cttatctgca aagcgcccgt ttatctccgc agcccccggc caggcagacc
      361 gaacaggcct gcgcctgctg atagaatacc acccaaaagc cgcagaggac actgaaaacg
      421 gaaaacgtac taaccacttt cctcacctgc gtaacttgct ccttccaacg accaggaaac
      481 gccgtacggt cacttcacga aatatactaa ccttccccga atcacgttag tttctcacaa
      541 ggttcacgga ttgcttagat gcacggcggc ggggcaccga acaccacgtt ctgaaccagg
      601 gcgacaatat cgataatatc caaaacgcca tcgcagttca aatctccggt ggcgtccggc
      661 gtagccaaga gggcgccgaa aaccacccgt tgaatcaaac tgacgaggtc actgatgtcg
      721 accaccccgt cgcgggtcac gtcgccgacg gcgagggcct gattgcagta gaggttcagc
      781 ccgccgtcgc tttccccagc aaccagatcc ggatcgccgt cgtaatcaat gtcaaccaga
      841 aagggagcgg cggtctggcc cacatcaata ttctcaaagt tctcagtttc tttgacgaaa
      901 ttgaaggtgg ttgaatcccc gctattgcgg tagaaccaaa tcgttccgtc gcttgtaccg
      961 agaaagagat cgtagtcccc gtcgccgtca atgtccgcaa aagccggcag ggtctgctgc
     1021 agaccagtgg gggggagtaa tgccgtcgct tcctgtacga aattcggcgg aaagggtccc
     1081 ccctcgttgc gatagaaata gagattggca ttggcctgat tatcccattc cccgacgaac
     1141 agatctaaat cgccatcgtg gtctatgtcc accagaaccg gagaagcatt gaaatcgaga
     1201 tccaacccga ggaagaaacc gtcggtcagg gtaaaaacgg gattgctcgg cgtgccggta
     1261 ttgcgaaaga gcgccagccg gccatcttcg ttgccgcaga tcatatcgag atcgccatcc
     1321 ccgtccagat cgcccaaagc cggagtgctg gaggcaccca catctacgcc gccgaagccg
     1381 ctggaaacga attgccaggc agggtgacgc tggctgccga cattccggta ggcggccatc
     1441 cggccgtcgt cccggccgca gaagaggtcc ggcaggccgt ctccgtccca gtccaccacc
     1501 gctggatagg agttggcgcc aaagtccaga ttcgagagca ggttcctggt aatcaaggat
     1561 tcccgcggcg ctgtcgggct accgacgtta cggaaataca agaggttatc gatgctcgaa
     1621 ccgaagttgg ccgatcccac gaccagatcc ggatccccgt cggcgtccag gtcggcaaaa
     1681 gccggctcgt tgaaaccgat cgtgctcacc ggcaaaaagg tgcaggtgat cgcctggagg
     1741 tgggagttca gcggggtgcc ctgattccgg aaaaaataca tgttcgtgtt gaaaatgtcc
     1801 ccccagaaga aatcgaagtc gccgtccccg tccagatcgg cgaactgaat gccgttgatg
     1861 ccgtgggcct gtgcttcagc cagttggccg ggaggcggcg gtgccacgga aaccgagcac
     1921 ccgacggtcg gaaaggcgac gatggaatcg aaatcgttcg cgccgaagac gaaattgggc
     1981 gaaagcgggg tgccgctgtt ccgccagtac gtcagggtgc ccgtcaattc ccccagaaag
     2041 aagtcgaaat cgccgtcacc gtcgatgtcg gcaaacgcgg gggtgttatt gaagccggag
     2101 aagatgttct gaaagttctg actgatttta ttgaaaatga aactgtccgg cgggctgtca
     2161 ttgcggtaga agcgcatgcc gccggtgttg ttgtccacga acaggtcggg atcgccgtcg
     2221 ccgtcgatgt cggccaagcg gaaccactgc ccggcgttta tccccaaaaa agaatcagcc
     2281 ccgtaatcaa attgcggcga actgagggtg ccggtgttcc ggaagaagac cagccgcccg
     2341 tctttctgca agatgaccag gtcaaaatca ccatcccggt cgaggtcgac taattggggc
     2401 ttggggctgt caataccccc caaaaacggc atggccaccg gcccggcact gtcgtttatc
     2461 gggaaactgg tgaccgaacg caggaaaggt tgggcctgga cgcctgccac ccagatcaga
     2521 agcaaaccga ccacgggaaa aagccgttgg ccccgaccaa aatgcctttt atctaataga
     2581 ccggaaaaca cccgttattt gagtcagatt gatgaacacg agttgctttc gacttaccca
     2641 tccgtccgag acgaatcctt gtggtctcca tattgctatt aaaactacgc tttcactctg
     2701 cctgatgcta ccagtatttt cgatcgggta aaccctgtca aaaaaatttt catgtcttcg
     2761 gaaaaaatac ttgacaaatt ctcactacta ttgtaaatta ctgaataagg ttagattttg
     2821 cgcatgcgtt tgggcgggcc cacttttgtt tttccacttc ccatcaataa gaacttgtgc
     2881 taccagtgta accaatttgc gaggtaaaag gttaagtggc ggatgggcat cgccttaagc
     2941 agaaatgatc gtatcatacg cgaaaatccg aggaggtgaa ttcgattggc cagaggtaag
     3001 gtcaagtggt tcaatgaaaa aaagggattt ggcttcatcc aacaggatgg tggaggacag
     3061 gacatttttg tccactacac tgccataaag gcagagggat tcaaatcctt ggttgagggg
     3121 gatgaggtag aattcacagt tcaggaaggt cctaaaggac cacaggcaac gaatgtgagc
     3181 agagtggttg cggagggtaa ccgggcactg taaggtttag ctttctaccg aaaagcgaaa
     3241 aggccaggaa tattcctggc cttttcgctt tttcccgccg gtcacaagcc gaacttgcca
     3301 aaaggtaaaa tcagattgaa gaatcggagc gccaatttgt tttcagtggc aaatcggcgc
     3361 aaaatggaaa gcaccccgta caacccaatc acaaaaaata tcgtcccgaa gatggtgagc
     3421 acatagcagg cgaccagcgg tccgccgccg gcaaagttcc ggatttcggg gtactgtgtt
     3481 gcatgtttcc cgaggacgat caatcctagg ccaaaccaaa aaaacccacc gaagattttc
     3541 agccagtttt gcatcatttt acctctctcc tttccctctg tatcctctaa acgcaagccc
     3601 aatgcccgtc tacaagaaca ttaaggactc ccggaaccac ttaaatgact acgggttaac
     3661 ccaattacca cgctgaatgc ttaattgtcc ggattttata gaagatggta atgggaaacc
     3721 gattgcagaa ccgaaagaag tcgcggcatc tcactgcgtt gagattgttc gatcatagtg
     3781 gagcgaaata aatggaggcg gtgggagttg aacccacgtc cgtgagaaat tcaggagcgc
     3841 tctctccctg cacagtccgg cttttgcttt cgcctgtggg aacgccgcca gacaggcttg
     3901 gccgcaggct atccctattg agtttcgcgc cggaagttcg ggaaccttcc ggggctatcc
     3961 cttctggctc gacgccaagt tcctgaccga agggcagatc cggagttgac gggttgcgta
     4021 ggttttagtt acgcagccag tgcatacgcg ttcgcgttgg cacttgtaca tgtcccaggg
     4081 gttttacgag gtcacccggg cacctcggca ggctggcact ccctccttat ctccgtcgaa
     4141 tcccgtcgcc cccaatccag cgactcttcg ctcggagtcc agaaagaacc agaactctca
     4201 ttcttaatat acgggaaaac gccttccccg ataagttctt ctgtgtcgcc tccccacgca
     4261 acctcataaa tgcgccttgc aggattcgaa cctgcgaccc actgattaag agtcagttgc
     4321 tctaccagct gagctaaagg cgcccaatgg ttatatatca ctaatcccta atcactcgtc
     4381 cctgtatttc ctgagggtgg gtgggatcga accaccggcc tcctgcttaa aaggcagatg
     4441 ctctaccact gagctacacc cccggatttc atccgaatat acgagaccga aaatggtcgt
     4501 caaagaacat ctacgacgcc tgaagccaga tcgtctcctc ccgccggtcg ctggtggaaa
     4561 ccaataacat cggcacaccc aacaactttt gaatcttttc tacgtaccgt cgggccggct
     4621 cgggcaactt cccgtagacc gtcactccgc tggtaacgcc gctccagccc ggataagtct
     4681 cgtagactgg ctcacagtcc cctagggccg ccagatcgac cggcagctct ttcagttgct
     4741 cgccgtggaa cttgtaggcg atgcaaattt tgagttccgg caatccatcc agcacgtcca
     4801 gtttcatcaa ggcgattttg gtgatgccgt tgacccgcac tgcatatttg agcgctaaga
     4861 tatcgagcca gccgcagcgc cggggccggc cggtggtggc gccgaattcg ttcccgcgct
     4921 tctgcaattt ctgaccgact gcgtcggtca actccgtggg gaaaggcccg ttccccaccc
     4981 gggtggtgta ggccttcatc acgccgatta tttcatccac gcacctgggg ccgacaccga
     5041 ggccggtcat cgccccgcca gcgatcgtat tcgaagacgt ggcgaaaggg taggtgccga
     5101 agtcgacatc caaaagcgtc ccctgcgccc cttcaaagag cacgtttttt cccgctttga
     5161 tggcgttgtg gaggtagagc gacgtgtcgg tgactaaggg cttgatccgg tcgcgatagc
     5221 cgcaggccag atcaaccagt ttcttttcat tccaatcgaa tttgccattg gtcgcctcta
     5281 tcagtggacg tttccacgca agattttcgg tgaattttgc ggccaaacgc ttttcatccc
     5341 agaggtcaat cacccggatg cccaggcggg cgaacttgtc ggtgtaggcc ggaccgatgc
     5401 cgcgcccggt ggtgccaatc ttttctctct ggtgggactc ctgactctgc tccaacgcct
     5461 tgtgatacgg gaggaccaag tgcgccgccc cgctgatgaa tagtcgtccc gagacgccga
     5521 tctggcgctt ctctaactcg tcgatttcgg tcaccagctg ctccaaatca accaccacgc
     5581 cgttgccgat gacgcagatc ttcccggggt ggagaatgcc ggaagggatc aaatggagaa
     5641 tgaatttttc ggagccgatg acgaccgtat ggccggcgtt ggcccctccc tggtagcggg
     5701 caatgatgtc ggcctcgcgg gaaaggagat cgaccacctt ccccttccct tcatcccccc
     5761 actgcatacc gacgacgatg cgattaggca tgatgctccg tcatttcttc atctccacga
     5821 cccgctcgcg agtgccgaac ttctctttct gcatctcggt aatcccggat aacctgtcca
     5881 atccaccgca aaacctcatc ccgcccgcga ccactcgtcg ccgaaaacag agtgattttc
     5941 tccggcgccg agggggagaa agcctcctgc gcccgcttga gggcctgcgc ctcttcgccg
     6001 cgggacagtt tatccgcctt ggtcaagacc accagaaacc gcagaccaat atgcgagagc
     6061 cactccgcta actcaaggtc atccgggaaa ggcggatggc gactgtccac aatctgaatg
     6121 accctggcca agtctctggt ttcctggaga tacttctcaa ccaaagtccc ccactgctcc
     6181 cgaagcttct tttccacttt ggcgtagccg tagccgggaa gatcggtgaa ccagatctgc
     6241 tcattgacca gaaagaaatt gagcg
//
LOCUS       DBNGZA010000277         4479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_475053, whole genome shotgun sequence.
ACCESSION   DBNGZA010000277 DBNGZA010000000
VERSION     DBNGZA010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_475053"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16FU_05920"
     CDS             <1..287
                     /locus_tag="AC16FU_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2993941.1"
                     /translation="EEIRDRVFEPFVTYGKTRGTGLGLSFAKKIVEAHGGTIAFTTLP
                     GKGSTFSVTLPRQTIPETESPGSGAAPGEESTQKVIQKRVQAILTSPEKR"
     gene            292..1362
                     /locus_tag="AC16FU_05925"
     CDS             292..1362
                     /locus_tag="AC16FU_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR2993942.1"
                     /translation="MEAPESRTKEYYQTSLRPQAEEAVFSLDRHGCVTFWSQEAQKLY
                     GLSREGVLGRSLTVLVREEKQPELRFALDRVLAGEELVRLLVPHEKCHGRVKQVEINL
                     SPVKDRQGEVLAVSCFAHDISAKREVEEHRLAETHLQAKVDSLKVVIATLSHYLNNTM
                     AVILGRTQMIDHCVGPEGITDRTGKLAPALTSIRKGTLKIAAVLEELRELSMSDHPQY
                     FKNSQAFDLEHRLDERLKKMFPEFPELTTNGKNHTSSPANTGTDAPVQSEAGQAEPKE
                     NISAKSSNAEIGLAPHPLAKPDSVESPAEPLRLSLRAVTQPATPPTLAVPAARERGAE
                     RRWQKALGRSLRKLLLRDESDF"
     gene            1470..2615
                     /locus_tag="AC16FU_05930"
     CDS             1470..2615
                     /locus_tag="AC16FU_05930"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR2993943.1"
                     /translation="MRFVVLGAGRMGQAIAFDLARTPEVGEVVLADRNLQLAKRAASL
                     LKSPKVIPRRIDASASSDLVKFLRAADCVASALPYYLNLRAAKAAVSAKVNFCDLGGS
                     NAVVKAQLALDPQAKKAGVTVIPDCGLAPGLAGLLAANGIEQLDAVESVNLRVGGLPQ
                     HPKPPLDYLLTWSVEGLINEYTEKCTVIRQGKVASVDALDELEELTFPPPFGRMEAFH
                     TSGGASTLPQTFAGRVRNLDYKTIRYPGHCAKIKTLISLGLAESKPVPVDGQKVIPRA
                     FLKTLLEKHLEREGPDVVLVRVEVAGTHKGQKRKITYQLIDYFDTQTGLSAMMRCTGF
                     SIAVVALLLARGEITQKGALPQERAVPVDQYIAELRARGIGIKEEIL"
     gene            2673..3377
                     /locus_tag="AC16FU_05935"
     CDS             2673..3377
                     /locus_tag="AC16FU_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR2993944.1"
                     /translation="MHNFKEFEALSFDCYGTLIDWETGILNALRPVLASHNQTLDGER
                     ILKLYAEIEAKLEAGRYRTYKEILKSVVKELGRQLGFFPTTDELNCLVDSLRYWQPFP
                     DTVEALQVLKEKYRLAIISNTDDDLIALTAKHLKITFDWIITAEQVKSYKPSLRNFTF
                     ALERMGIAPAKVLHVAQSIYHDIIPARSLGLATVWVDRRKGRDGFGATPKAEGKPDLE
                     VPDLKTLVTHISNGQR"
     gene            3420..3950
                     /locus_tag="AC16FU_05940"
     CDS             3420..3950
                     /locus_tag="AC16FU_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993945.1"
                     /translation="MKRTSNLKFDVTPILQRAKHLTKPRVDGISINLPFISISLTVSD
                     NEKKIAREVLIRLRDKRVLVAWECCNNCINNALASIQDIRTLLVNKQVELPDEDSALF
                     LLFDLMLAGIRQFLTFTERFDPHIHREEYFGALGVLRGHLLRCIEEIAKVGNTTPKLS
                     NRLSFNPIWEKELYLS"
     gene            3970..>4479
                     /locus_tag="AC16FU_05945"
     CDS             3970..>4479
                     /locus_tag="AC16FU_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993946.1"
                     /translation="MRSYKTAAESNSEISQRYSHYINKVMRDNEKFLQYYAKDTSSEI
                     IELVNDAIDNVTFWAKRGDSENDYASHSIAFFIYHILMPFSYAMYVDLIAGNIPVCFM
                     ELRLMLESLVKCYSADLRYPNQTFFQEKLELLDKKKRKISQVMKEVGEMVGFQNNFVT
                     LWRKLSESWI"
BASE COUNT         1125 a         1166 c         1205 g          983 t
ORIGIN      
        1 cggaagagat ccgcgaccgg gtctttgagc cctttgtgac ctacgggaaa acccggggca
       61 ccggtctggg gctctccttt gccaaaaaga tcgttgaggc gcacggcggc acgattgcat
      121 ttaccacgct gccggggaag ggatcgacct tctcggtaac gcttccccgt cagaccatcc
      181 cggaaaccga atcgcccggc tccggtgcgg caccgggtga agagtcgacg cagaaggtga
      241 ttcagaagcg tgtccaggcg atactcacgt cgccagaaaa gcggtgaaac gatggaagcc
      301 ccggaatccc gcacgaaaga atactaccag accagcctcc ggccccaggc ggaagaggcg
      361 gtcttcagcc tcgaccggca cggctgcgtc accttctgga gccaggaagc ccagaaactc
      421 tacggccttt cccgtgaggg ggtgctgggc cggagcctga ccgttctggt ccgggaagag
      481 aagcagccgg aactgcgctt tgcgctagac cgggtgctgg ccggtgaaga actggtgcgg
      541 cttttggtgc cccacgagaa gtgccacggc agagtcaaac aggtggaaat caatctgtca
      601 cccgtcaagg atcgtcaggg ggaggtgctg gccgtctcct gcttcgccca cgacatcagc
      661 gccaagcgcg aggtcgaaga gcaccggctg gccgaaaccc acctgcaggc caaagtcgat
      721 tcactcaaag tggtgattgc cacgctctcc cactacctca ataacaccat ggcggtcatt
      781 ttgggccgga cccagatgat cgaccactgc gtgggtccgg aagggatcac cgaccgcacc
      841 ggaaaactgg ccccggcgct cacgagcatc cgcaagggaa cgcttaaaat cgccgccgtt
      901 ttggaagaat tgcgggaact ctccatgtcc gaccatccgc agtacttcaa aaactcccag
      961 gccttcgacc tggaacaccg gttggacgaa cggctgaaga aaatgtttcc ggaattcccg
     1021 gaattgacca ccaacgggaa gaaccacacc tcatctccag cgaacaccgg cacggatgca
     1081 cctgtccaaa gcgaagcggg ccaagcggag cccaaagaaa acatctctgc gaaaagttcg
     1141 aacgcagaga tcggattggc tccccatcct ttggcgaaac cggatagcgt ggagagtccg
     1201 gctgagccgc tccgtctttc tcttcgggcc gtcacccagc cggcaacccc accgaccttg
     1261 gcggtgcctg cggcccggga gcgcggcgcc gagcgccgct ggcaaaaagc actgggccgt
     1321 tccctgcgga aattgctcct gcgcgacgaa tctgacttct aaacgtttct ctttcgctcc
     1381 cggctcggat gcgacttaac tttagttcat tcaagtaggg gaagcgtcta tttgatcaat
     1441 ttgcttcggt ctgttttatt ccactggcaa tgcgcttcgt agttttaggt gccggccgga
     1501 tggggcaggc cattgccttc gatctggccc gcaccccgga agtgggcgaa gtggtgctgg
     1561 ccgaccgcaa cctccagctg gccaagcggg ctgcctcgct tctcaaaagc ccaaaagtca
     1621 tcccccggcg aatcgatgcc tccgccagtt ctgatctggt gaaatttttg cgcgcggccg
     1681 actgcgtcgc cagcgccctg ccgtactacc taaatcttag agcggcgaaa gcggccgtca
     1741 gcgcgaaggt caacttttgc gacctcggcg gcagcaacgc cgtcgtcaaa gcgcagttag
     1801 cgcttgatcc acaggcgaaa aaggcggggg tgacggtcat ccccgactgc ggcctggcgc
     1861 ccgggttggc cggccttttg gcggcgaacg ggatagagca attggacgca gtcgaatcgg
     1921 tgaacctgcg cgtgggcgga ttgccccagc accccaaacc gccgctcgac tacctcctca
     1981 cctggtcggt ggagggactc atcaacgaat acacggaaaa gtgtaccgtg atccggcagg
     2041 ggaaagtagc ttccgttgac gctctcgacg aattggagga gttgaccttt ccaccgcctt
     2101 tcgggcgcat ggaggccttc cacacctccg gcggcgcttc gaccttgccg cagacatttg
     2161 ccggccgggt gcgcaacctc gactacaaga cgatccgcta ccccgggcac tgcgcaaaga
     2221 ttaaaacgct catctcgctg gggctggccg agtcaaaacc agtgcccgtt gacggtcaga
     2281 aagtcatccc ccgggcgttc ttgaagacgc ttctggagaa acacctggag cgggaaggac
     2341 cggacgtggt tctggtgcgg gtggaggtcg cgggaacgca taaaggacaa aagcggaaaa
     2401 tcacttatca actgattgac tactttgata cccagaccgg tctttcggcc atgatgcgct
     2461 gtaccgggtt ttccatcgcc gtggtggcgc tcctcctggc ccggggagag attacacaga
     2521 agggcgccct tccccaggag cgtgcggtgc ccgttgacca gtacattgcc gagctgcgcg
     2581 ctcgggggat tgggatcaaa gaagaaatcc tttgattttg ttttgtaggg gttcaatata
     2641 ttgaacccct acgattattg gatcaaatcc caatgcataa tttcaaagaa tttgaggcct
     2701 tgagcttcga ctgctacgga acgctcatcg actgggaaac tggtatcctc aacgcgttgc
     2761 gcccggttct cgccagtcac aaccagaccc tggatggcga acgaattctc aaactgtatg
     2821 cggaaatcga ggcgaaactg gaggcaggaa gataccgtac ctataaagaa attctcaagt
     2881 cagtagtcaa agagctcggt cggcaactcg gcttttttcc aaccaccgat gagctcaact
     2941 gtctcgttga ttctctaaga tactggcaac cgtttcccga tacagttgag gcactgcaag
     3001 ttttgaagga gaagtatcgc ctggcgatta tttccaatac cgatgacgat cttattgcct
     3061 tgactgcgaa acatctcaaa atcaccttcg actggatcat caccgcagag caggtaaagt
     3121 cgtataaacc ttcgttacgc aatttcacct ttgcgctgga gcgaatgggc attgctccgg
     3181 caaaggttct ccacgtggca cagagcatct accatgatat aatccccgcc aggagtctgg
     3241 gattggcaac cgtctgggtc gaccgcagaa aagggcggga cggatttgga gcgactccga
     3301 aggcggaagg gaagcccgat ctggaggtgc cggatttgaa gacgctggtt acccatatta
     3361 gcaatggtca acgatgaaaa gattatattc agctaagtaa ggtcgggtgg aggacttaaa
     3421 tgaaaagaac gagcaatttg aagtttgatg taacacccat tttgcaaagg gcaaagcacc
     3481 taacaaagcc gagagttgac ggcatttcaa ttaatcttcc tttcataagc ataagcttga
     3541 ccgtttccga taatgagaaa aaaattgcca gagaagttct tattcgacta agagacaagc
     3601 gtgttctcgt agcgtgggaa tgttgcaata actgcataaa taatgccctg gcttccatac
     3661 aggatattcg aactctactt gttaacaagc aagtagaatt acctgatgaa gattcagctt
     3721 tatttctact ttttgattta atgttggcgg gaatacgcca gttcttgact tttacagaac
     3781 gatttgatcc tcatattcac cgagaagagt attttggggc cctgggtgtg cttcgcggtc
     3841 acctcttaag atgtattgaa gagattgcaa aggtcggaaa tactacgcca aagctaagca
     3901 atcgacttag tttcaatccg atatgggaaa aagaactgta cctttcttaa gaactctggc
     3961 cggtaaaaga tgaggagtta taaaacggca gcagaaagta attcagaaat ttctcaaagg
     4021 tactctcatt acattaacaa agtgatgaga gataatgaga agttcctcca atactatgca
     4081 aaagatacga gtagcgaaat catagaactt gttaatgatg caatagacaa tgtaacgttt
     4141 tgggccaaga gaggagatag tgaaaatgat tatgctagcc attcaatagc ttttttcatt
     4201 tatcatattc tcatgccgtt cagttacgca atgtacgtgg atttgatcgc aggtaacatt
     4261 cctgtatgtt ttatggaact taggttaatg ctcgaatctt tagtgaagtg ctattctgca
     4321 gatttaagat atcctaatca aacttttttt caagaaaaac tcgaactatt ggacaaaaag
     4381 aaacgtaaga tatcccaagt tatgaaagag gtgggagaga tggttggatt tcaaaataac
     4441 ttcgttactt tgtggcgcaa actttctgaa agttggatc
//
LOCUS       DBNGZA010000278         1716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_262470, whole genome shotgun sequence.
ACCESSION   DBNGZA010000278 DBNGZA010000000
VERSION     DBNGZA010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_262470"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /gene="pstC"
                     /locus_tag="AC16FU_05950"
     CDS             <1..690
                     /gene="pstC"
                     /locus_tag="AC16FU_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR2993947.1"
                     /translation="PLFLGTLKASFVALFFATPLAILAAIFTSEFAPPRLGRMIKPAV
                     ELLAGIPSVVLGFFALIVLASWIQDAFHLTMRLNATVAGIALGIAVIPIIYTVAEDAL
                     ATVPKAYREASFSLGATSWQTAVKVVLPAALPGIFAAMVLGFGRAVGETMIVLMASGN
                     ASIVSASFADSIRTLSATIAAELAEVVFGSGHYRLLFGIGVLLFAFTFVLNLVGHWYI
                     QRVKRRLLGVA"
     gene            687..1577
                     /gene="pstA"
                     /locus_tag="AC16FU_05955"
     CDS             687..1577
                     /gene="pstA"
                     /locus_tag="AC16FU_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR2993948.1"
                     /translation="MKDRVFVALSAVATLLILVMLAVILGNIFWFAKDRLSWEFFSAS
                     PRVGMTEGGIFPAIFGTCALVLLMTIAVMPAGVLTAIYLEEYARADSKMVQVVRQAVA
                     NLAGVPSIVFGLFGLGFFINFVGGGVDGILFGGELNYGQPAIIWAAATLALLTMPVVI
                     VTTSEALRAVPKEIKEGGLALGATRLQNLRRIIIPSALPGILTGGILAVSRGAGEVAP
                     IMFTGAAYYLPYLPTKPNDQFMELGYHVYVMATQSPDVEKTKPILYATVFVLLALTFL
                     LNLLAIWLRARLRRRFAEER"
BASE COUNT          293 a          472 c          518 g          433 t
ORIGIN      
        1 cctctctttc tgggaacgct caaggcctcg ttcgtggcgc ttttcttcgc aacccctctg
       61 gcaatcctgg cggcgatctt cacttcggag tttgccccgc cccgcttggg acggatgatc
      121 aaacctgcgg tggaactttt ggccggtatc ccctcggtgg tgctcggatt ttttgcctta
      181 atcgtgctgg cgagctggat tcaggatgcc tttcacctga ccatgcgcct caatgccaca
      241 gtcgcgggaa tcgctctggg aatagcggtc atcccgataa tctacaccgt ggcggaagat
      301 gcgctggcaa ctgtccctaa agcctaccgc gaggcgtcgt tctctttagg agcgacgtcc
      361 tggcagaccg ccgtgaaagt cgttctgccg gcggccctgc cggggatttt tgcggcaatg
      421 gttctggggt tcggtcgggc agtgggcgag acgatgatcg tacttatggc ttccggtaac
      481 gcctccatcg tcagcgcgag ttttgccgat tccatcagaa ccctctcggc gacgatagcc
      541 gctgaattgg ccgaggtagt gttcggaagc ggtcactacc ggcttctgtt cggaatcggg
      601 gttctcctgt ttgcgttcac gttcgttttg aacctcgttg gccactggta catccagcgg
      661 gtgaaaagac gccttttggg ggttgcgtga aggatagagt ttttgtcgca ctgagtgcgg
      721 tggctaccct gctcattttg gtcatgctcg ctgttatcct cgggaacatt ttctggttcg
      781 cgaaggaccg cctctcctgg gagtttttct ccgccagccc gagagttggc atgaccgaag
      841 ggggaatttt cccggccatc ttcggcacct gtgccttggt gctcctgatg accatagcgg
      901 tgatgccggc cggtgtcttg accgctattt acctcgaaga atatgcccgg gctgattcta
      961 agatggtcca ggtggtccgt caggcggttg cgaatctggc gggagtgccc tccatcgtct
     1021 ttggtctctt cggcttgggg tttttcatca atttcgttgg tggaggcgtt gacggcatac
     1081 tcttcggagg tgaactgaac tacgggcagc cggccatcat ctgggcggcg gcgactttgg
     1141 ctcttcttac gatgcccgtc gtgatcgtga ccacgtccga agcgttgcgg gccgtgccga
     1201 aggaaatcaa agagggcggc ctggctctgg gagcaacgcg cttgcaaaat ttgcggcgca
     1261 ttatcatccc ctcggctctt ccggggattt tgaccggcgg tattctggcc gtttcccggg
     1321 gggccggtga ggtggccccg atcatgttca ccggagcggc ctactacctg ccgtacttgc
     1381 ccacgaagcc aaacgatcag ttcatggaat tgggatacca tgtctacgtg atggcgactc
     1441 aatccccgga cgtcgagaaa accaaaccaa ttctgtacgc gacagttttc gttttgctgg
     1501 ctttgacgtt ccttttgaac cttctggcca tctggctgcg agcacgtctg cgccggcggt
     1561 ttgcggaaga acggtaatgg aactccctga cgtggaatgg gaaacggtgc ctgagggtcc
     1621 gcggttaaag cttgagccca ccagacggaa tgcggccgat gcggctgagc aggagaaaat
     1681 cgtcgtcgaa cgcttttcct tctggtacac gaaaac
//
LOCUS       DBNGZA010000279         1446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1852153, whole genome shotgun sequence.
ACCESSION   DBNGZA010000279 DBNGZA010000000
VERSION     DBNGZA010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1852153"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1112)
                     /gene="waaF"
                     /locus_tag="AC16FU_05960"
     CDS             complement(93..1112)
                     /gene="waaF"
                     /locus_tag="AC16FU_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR2993949.1"
                     /translation="MVIRDDRIGDLVLATPVCEALRKKFPESAVTVLASTSAGEILRH
                     NPSVNAVIERSEAAGRIFSKAFWKLVRRVASEHFHLAVILKPSWHTAFLTTFAKIPLR
                     IGTGYRPYSGLFNRRIYVHRKDSTRHELEYNFDVLQPLGITYQGEVPKIFLNEAERER
                     GRTLLEPLRQAESSLVVIHPGGGGGSARFWPVGHFAQLSDRLVEAGYRVVLTGSKADR
                     PVVSEYLGAVRLGPFRLDEKTILRELAAAYAACNLVITNSTGPMHIAAAVGTPVIAFF
                     CPIRTASPRRWGPRGVSHRVLLPLVTECTCAVNRCERGNCMAQITVEQAFASAVSVLT
                     SLSRR"
     gene            complement(1139..>1446)
                     /locus_tag="AC16FU_05965"
     CDS             complement(1139..>1446)
                     /locus_tag="AC16FU_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993950.1"
                     /translation="ELLHYTDPVLTHYLAKLDRYTGLSAQELFEQHYPFRIWDLLVRP
                     PAVFFKMYVWRAGFLDGIEGFLLALCSAIHVLMKYAKLWEYYHNPNARDLRSGNASP"
BASE COUNT          344 a          399 c          411 g          292 t
ORIGIN      
        1 ttcatagtcg gcccttaggt ttttccgtca acgtgcctct gacagaatat acgccctccc
       61 tcctgcgagg gtaagagcgt gatctgttaa gattatcgac gggaaaggga ggtgagcaca
      121 gaaaccgcgc tggcgaaagc ctgttcgacc gtgatctgcg ccatgcagtt gccgcgctcg
      181 caacggttca ccgcacaggt acactcggtg acgaggggga gcagaacacg gtggctcact
      241 ccccgcggcc cccagcgtcg tggggaggcc gtgcgaatcg ggcaaaaaaa ggcaatgacg
      301 ggtgtgccca cagcggcggc gatgtgcatg gggccggtgc tattggtgat gaccaggttg
      361 caggcggcat aagcggccgc cagttcacgc agaattgttt tttcgtccaa gcggaatggc
      421 cccagtcgta ctgcgccgag gtactcagaa acaaccggac gatctgcttt tgagcccgtg
      481 agcaccaccc ggtatcccgc ctccaccaga cggtccgaaa gctgcgcaaa gtggcccacc
      541 ggccagaagc gggccgaccc cccaccgccg ccggggtgaa tgacgaccaa agacgattcg
      601 gcttgccgca ggggttccag taacgtccgc ccgcgttccc gttccgcttc gttgagaaag
      661 attttgggga cttctccttg ataagtaata cccaatggtt gcagtacatc aaaattgtat
      721 tccagctcat gccgggttga atctttccgg tggacgtaaa tccgccggtt gaaaagtccg
      781 ctgtacgggc ggtagccggt gccgatccgg agcggaattt ttgcaaaggt tgtcaaaaag
      841 gccgtgtgcc aggagggttt caatattacc gccagatgga aatgttcgga agcaacccgc
      901 ctgaccaatt tccaaaaggc tttcgagaaa atccgccccg ccgcctccga ccgctcaatc
      961 accgcattga cacttggatt gtgacggaga atttctccgg ctgaagttga cgcgaggacg
     1021 gtaacggctg actccggaaa tttcttccgc agcgcttcgc aaaccggtgt ggctaagacg
     1081 agatcgccaa tccggtcatc acgaataacc aaaatttttc tcggagcgtt ctgcaaaact
     1141 atggagaggc gtttccggaa cgcaagtctc tcgcgttcgg attgtggtag tattcccaca
     1201 atttggcgta cttcataagg acgtggatag ccgagcacag agccaaaagg aatccctcaa
     1261 tgccatccag aaagcccgcc cgccagacgt acatcttaaa aaaaactgcc ggcggacgaa
     1321 caagcaaatc ccagatgcga aagggatagt gttgttcaaa cagctcctgc gcggagaggc
     1381 ccgtgtaccg atctaatttt gccaagtagt gagtgagaac cggatcggta tagtgaagca
     1441 gttctc
//
LOCUS       DBNGZA010000280         5132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1745636, whole genome shotgun sequence.
ACCESSION   DBNGZA010000280 DBNGZA010000000
VERSION     DBNGZA010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1745636"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="AC16FU_05970"
     CDS             <1..1163
                     /locus_tag="AC16FU_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR2993951.1"
                     /translation="LIVDERGYILTNEHVVDNAQSVTVTLPSKREYPAEVVGVDKQTD
                     LALLKIDAPEKLTAAPLGNSDDVQVGEWVIAVGNPYGFDRTVSFGVVSGKGRVLPRLS
                     MDMPLINNFIQTDAAIDPGSSGGPLVNLQGEVVGINSMGVGRGQGFTIPINIAREVKN
                     KILTSGVIERGWIGIVMQPFPRDFARYFSQPDLEGILVSDVLENSPAAGAGLKPGDVI
                     TRFNGREVSAEKDEDLNSFSLTVTQTNIGDRIPVSVSRAGKPVELSITIGTQPKVKAE
                     EFETGLGFTVKEITESIFLQSGLADKDGVLVSYVEVGGEASKAQLGEGDVVHSVEGET
                     VADLAGFKATLAKVKEKPQILLTVKRGKNNRYIILNHSPARGVGQTPELPQN"
     gene            1310..1786
                     /locus_tag="AC16FU_05975"
     CDS             1310..1786
                     /locus_tag="AC16FU_05975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2993952.1"
                     /translation="MNILHVLSGDFVSGAETYAAALIDEQMRRGHNVFIASGSFSQPT
                     GARFIVLPIYQRNYIRRVRNVLTLLRLIRREGIDIIHAHSRAACWVSHWAAKFTNTAF
                     VTTLHGRLHRRRSLLAYNVYGNTCIAVCEKIRDQLLSETASLRPEQVVVIRNGIRL"
     gene            1770..3071
                     /locus_tag="AC16FU_05980"
     CDS             1770..3071
                     /locus_tag="AC16FU_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2993953.1"
                     /translation="MASGSKPVVLVVGRLSGQKNRVIVNTLKFAAPEVVKVFPQAQFQ
                     VIGSPVQAEHRHLEKQHGFIRFYGHQPDLRSFYEDATIVVGAGRVALEAMALKKAVVA
                     IGERLYVGPILAEVIEQAKATNFGDCFEKEVFDWPRMSQDIVRLLHNDRLREQVALTG
                     YELAQSEYDLQKIYLQIEAVYQQAVLKNNLARKYEIPVLQYHRVVAEIPGKSRFNIYV
                     TRADFEKQLAVLKRHSFTPITFSDFLSRRLPQKPIILTFDDGYADHYHIAFPLLKKYG
                     LKAVIYILGNRNITSNCWDVPLGEPEVPLLKPEQIREMAESGLVDFGGHSMDHRNLIA
                     LSTTEMEREIVDSKKSIEEMIGQPVASFAYPYGSYNGKVKECTAAAGYIFGVASDRGP
                     ARFGADLFAIRRIPIFPHTSYWQFRKKTSGYYLRYRTLLGK"
     gene            3160..4683
                     /gene="gpmI"
                     /locus_tag="AC16FU_05985"
     CDS             3160..4683
                     /gene="gpmI"
                     /locus_tag="AC16FU_05985"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR2993954.1"
                     /translation="MVILIILDGFGLNPKREANAIALAKKPNLDRLFATYPTTQLEAS
                     GPAVGLPPGQMGNSEVGHLNLGAGRIVYQEITRIDRAIADGSFFENRALVGAMERCRR
                     NKSRLHLMGLVSDGGIHSSQEHLYALLDLARRKGAGEVYIHAFLDGRDTMPTTGAGYL
                     KQLQEKCRSLGVGKIASVMGRWWAMDRDKRWDRTTGAYLAIVKGEGKIASDPVRAVEE
                     SYQANVTDEFVAPVVITENGDPREGRIADGDEVIFFNFRADRTRQISHLLVDQDRPVA
                     GVNPKPEVNLTAMTHYDDTLKVPEAFPAQFVTNTFGELVSRAGLKQLRIAETEKYPHV
                     TFFFNGGNEAPFPGEERILIPSPKVATYDLQPEMSAPEVTRKVVEKIGAGEFDVIILN
                     YANPDMVGHTGVLPAAIKAVETVDGCLAQVLRAVEEVNDSAILTADHGNCDLMVDAKT
                     GGPHTAHTTNPVPCAVIHSGFSGKLRAGGILADVAPTLLEMLHLQPAPEMTGKSLLH"
     gene            4745..>5132
                     /locus_tag="AC16FU_05990"
     CDS             4745..>5132
                     /locus_tag="AC16FU_05990"
                     /inference="COORDINATES: protein motif:HMM:NF039484.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993955.1"
                     /translation="MLNKRWTPSARFEFGLVVGSALLLTLAYPPFPFGFLAYFALAGL
                     LWAIEGKSVAQAFRFTFLFGFVNHGTLLYWVGWAVRPQILGALGAVAAVLVLSLYLAI
                     TVGLFQFVRRRLVGIDPYRGSWAIVLF"
BASE COUNT         1118 a         1432 c         1489 g         1093 t
ORIGIN      
        1 gcttaatcgt tgacgagcgc ggctacattc tcaccaatga gcacgtcgta gacaacgccc
       61 agtcggtgac cgtcactttg ccctccaaac gcgaataccc ggccgaagtg gtgggggtgg
      121 acaagcagac cgacctggcc ctgttgaaaa tcgacgcgcc ggaaaaattg accgccgcac
      181 cgctgggcaa ctcggacgac gtccaggtcg gggagtgggt cattgccgtg ggcaatcctt
      241 acggctttga ccgcacggtg tcgttcgggg ttgtgtccgg aaaaggccgg gtgctccccc
      301 gactgtcaat ggacatgccg ctcatcaata acttcatcca gactgatgcg gcgatcgacc
      361 ccgggtcctc cggcgggcct ctggtcaacc tgcagggaga agtggtcggg atcaattcca
      421 tgggggtcgg gcgcggccag ggattcacca ttccgattaa catcgccaga gaagtgaaaa
      481 acaaaatcct gacttccgga gtgattgagc gcggctggat cgggatcgtc atgcagccgt
      541 tcccgcgcga tttcgcccgg tacttcagcc aacccgactt agaggggatt ctggtctccg
      601 atgttttgga aaattccccc gccgcaggag ccggcctcaa accgggggat gtcatcaccc
      661 gcttcaacgg gcgggaagtc tccgcggaaa aagatgagga tttaaatagt ttttcgctta
      721 ccgtgaccca gaccaacatc ggcgatcgaa ttccggtatc tgtctcccgc gcgggaaagc
      781 cggtggagct ctccatcacc atcggcaccc agccgaaggt gaaagccgaa gagttcgaaa
      841 ccgggctggg attcacggtc aaagagatca ccgaatcgat tttcctgcag agcggcctgg
      901 cggataaaga cggcgttctg gtttcctacg tcgaagtggg gggcgaggcg agcaaggcgc
      961 agttaggcga aggggacgtg gtgcattcgg ttgaagggga gacggtggcc gacttagccg
     1021 ggttcaaggc gaccctagcc aaagtcaaag agaaaccgca gatccttctc acggtcaaac
     1081 gcggcaagaa caaccgctac atcatcctca atcactcccc cgccagaggc gtgggccaga
     1141 cgcccgagtt gccccagaac tgagttcgct ttttattccc tctgcatgtc gcagggagtt
     1201 ctctcctatg cctcgtcgcc ggtgagaggt gaagtcctta aaccgtgcgt ggctattttt
     1261 ttgcagcccc aagcccttcg ggttctccac tgctgaatag agaaaaccgg tgaacatcct
     1321 gcacgtctta agcggcgact tcgtttccgg agccgaaacc tacgccgccg cgctcattga
     1381 cgagcaaatg cgcagaggac ataacgtctt tatcgcctcc ggaagttttt cccagcccac
     1441 cggggcaaga ttcatcgtcc tccccattta ccaacgaaac tatatccggc gagtccgaaa
     1501 cgtgttgacg cttttgcggc ttatccgtcg ggaagggatc gacatcattc acgcccactc
     1561 ccgcgccgcc tgctgggtga gtcactgggc agccaaattc accaatacgg ccttcgttac
     1621 caccctgcac gggcggctgc accggcgccg ttcgcttttg gcttacaatg tgtatggcaa
     1681 tacctgcatt gcagtctgtg agaagatccg cgatcaactg ctctccgaaa ccgcgtcgct
     1741 ccgccctgaa caggtggtgg tgatccgcaa tggcatccgg ctctaaaccg gtggtgctcg
     1801 tcgtcgggcg gttgtcgggc cagaaaaaca gggtcatcgt caacaccctg aaattcgcgg
     1861 cgcctgaggt tgtgaaggtt ttcccccagg cgcagttcca ggtcattggc tcgccggttc
     1921 aggcagagca ccggcactta gaaaagcagc acgggttcat tcgtttctat ggtcatcaac
     1981 ccgacctccg gtctttttat gaagacgcta ccatcgtggt cggtgcgggc cgggtggcgc
     2041 tagaagcgat ggcgcttaaa aaagcggtgg tggccattgg cgaacggctg tacgtggggc
     2101 ccattctggc ggaggtaatc gaacaagcga aggcaacgaa cttcggcgat tgttttgaaa
     2161 aagaggtttt cgattggccc agaatgtccc aagatattgt gcggctgctg cacaatgacc
     2221 gtctgcgcga gcaggtggcg ctgaccgggt acgaactggc gcagtccgaa tacgacctcc
     2281 aaaaaatcta cctgcagatc gaagcggtct accagcaagc ggttctcaaa aacaacctcg
     2341 cccgaaaata cgaaatccca gttttacagt accatcgtgt ggtcgctgag atacccggaa
     2401 aaagcaggtt caacatctat gttaccagag ccgattttga aaaacaactt gcagttttga
     2461 agcgacacag ctttacccct atcaccttct ctgattttct atcccggaga cttccccaaa
     2521 agcccatcat cttgaccttt gatgatgggt atgcagatca ttatcacatc gccttccccc
     2581 tgctgaaaaa atatggtctg aaggcggtga tctacatcct cggcaatcga aacatcacct
     2641 ctaattgctg ggacgttccg ctgggcgaac cggaagtccc gctcctgaaa ccggaacaaa
     2701 tccgggaaat ggccgaaagt ggcctggtgg atttcggcgg tcattcgatg gaccaccgaa
     2761 acttaatcgc tctctcgacg accgaaatgg aaagagaaat agttgacagc aaaaaatcca
     2821 tcgaggagat gataggacaa cccgtggcct cgttcgccta tccctatggg agttataatg
     2881 gaaaggtaaa ggaatgcacc gcggcagccg gctatatctt cggcgtcgct tccgatcggg
     2941 gtcccgcccg cttcggagcc gacttattcg ccatccgccg gatcccgatt ttcccccata
     3001 cttcttattg gcaattccgt aaaaaaacca gcggctacta tttgcgttac cggacgcttt
     3061 tgggcaaatg acgttttctc cgttaacccc gggactgtgg aaatggtatt tggcgaaaga
     3121 gtcacccgga gttaaatttg ccgtcagact tgtagcggta tggtcattct cattatcctc
     3181 gacggcttcg ggctcaatcc caaaagagaa gccaacgcga ttgccttagc gaagaagccg
     3241 aacttagacc gccttttcgc cacctacccc accacgcagc tggaggcctc cggcccggcg
     3301 gtgggtttgc cgcccggaca gatggggaac agtgaagtgg ggcatttgaa cttgggtgcg
     3361 gggcggattg tctaccagga aatcacccgc atcgaccggg ccatcgcgga tggcagtttc
     3421 tttgagaatc gtgctctcgt gggagcgatg gagcgctgcc gccggaataa atcccgtctg
     3481 caccttatgg gattggtctc cgacggcggc atccacagtt cccaggagca cctgtacgcg
     3541 cttttagatt tggcgcggcg caagggagcc ggtgaagtct acattcacgc ttttctggac
     3601 ggccgggaca cgatgccgac cacgggtgcg ggctacctga agcagttgca ggaaaaatgt
     3661 cgctctctgg gcgtcggcaa aattgcgagc gtcatggggc gctggtgggc gatggaccgg
     3721 gataaacgct gggatcgaac caccggagcg tacttagcga ttgtcaaagg cgaggggaaa
     3781 atcgcttccg acccggtgcg cgcggtggag gaatcgtacc aggccaacgt gaccgacgag
     3841 ttcgtggcgc cggtcgtgat aaccgaaaac ggggatccgc gggaaggacg catagccgac
     3901 ggcgacgaag tgatcttctt taatttccgg gccgatcgga cgcggcagat ctcgcacctt
     3961 ttggtggatc aggatcgtcc cgtcgccgga gttaatccga agcctgaggt caatctcacc
     4021 gccatgacgc actacgacga cacgctcaaa gtacccgagg ccttcccagc tcagttcgta
     4081 accaatactt tcggggaatt agtgagccgg gccgggctga agcagttacg cattgccgag
     4141 acggagaaat atccgcacgt caccttcttt ttcaacggcg ggaacgaagc gccctttccg
     4201 ggcgaggagc ggattttgat cccttcgccc aaagtggcga cgtatgactt acagccggag
     4261 atgagcgcgc cggaggtgac ccggaaggtg gtggaaaaaa tcggggccgg ggagttcgac
     4321 gtcatcatcc tgaactacgc caacccggat atggtggggc acaccggcgt acttccagcg
     4381 gccatcaaag cggtagaaac ggtggatggg tgcctcgcgc aagttctgcg cgcagtggag
     4441 gaagtcaatg actccgccat tctcaccgcc gatcacggca actgcgacct aatggtggat
     4501 gcgaagactg gcggcccgca tacggcgcat acaacgaatc ccgtgccctg tgcggtcatt
     4561 cattcgggat tttccgggaa attgcgcgct ggaggcattc tggccgacgt ggcgccgacg
     4621 cttttagaaa tgctccatct gcaaccggca ccggagatga cgggaaaaag tctgctccat
     4681 taaggagatt tggattttga gtcaaaccgt cgcaattcct gtgaaagaat cacataccgc
     4741 agcagtgttg aacaagagat ggacgccgtc cgcccgtttt gaattcgggc tggttgtcgg
     4801 ctcggcgctc cttttgactc tggcctaccc gccgtttccc ttcgggtttc tggcctactt
     4861 tgccctggcc ggattgctct gggcgataga gggaaagtcg gttgctcaag catttcgatt
     4921 cactttctta ttcgggttcg tcaatcatgg gacgctgctc tactgggtcg gctgggcggt
     4981 gcgtccgcag attctcggcg cgttgggagc ggtggcggcg gtgctggtgc tgtcgctcta
     5041 tttagcaatc accgtcggct tgtttcaatt cgtccgccgg cggctggtcg gaatcgaccc
     5101 gtacaggggc agttgggcta ttgttctttt cc
//
LOCUS       DBNGZA010000281         5385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1641263, whole genome shotgun sequence.
ACCESSION   DBNGZA010000281 DBNGZA010000000
VERSION     DBNGZA010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1641263"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="AC16FU_05995"
     CDS             <1..720
                     /locus_tag="AC16FU_05995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR2993956.1"
                     /translation="IGKVRHLLGPTCPIINIDHHPENERFGQINWLDTRAAAVGEMIY
                     EFLVAARHPISPAVATLLYTAILTDTGRFRYGSTRPQTLSICAALMAQGADPKVITDE
                     VYFNHPPANLRLIGQVVRNLELAYGKRVCALSIRRADLERFGAHFEDTEGIVDYSLFA
                     KGVAVGLLFKEVTPRKTKVSLRSQNHIDISRVARKFGGGGHANAAGCSVAAPLETAKK
                     QVLKEVIWYLRNGAGRNALNR"
     gene            689..1615
                     /gene="truB"
                     /locus_tag="AC16FU_06000"
     CDS             689..1615
                     /gene="truB"
                     /locus_tag="AC16FU_06000"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR2993957.1"
                     /translation="MAQDGMLLIDKPSGPTSHDVVDRVRAILKEKRVGHTGTLDPLAS
                     GLLVICVGRATKVARYLAGEDKEYQAVIRLGVTTTTQDRTGEKIQTRPVEGVTRADVE
                     AALPQFIGLQKQTPPAFSAVKVQGVPAYRSARRGEPVALTPREIEVFELQLTACEMPL
                     VTLRIRCSKGTYVRALAAEIGERLGTGAHLAELRRTKVGPWDVAEALTVDQLQQAAQE
                     QKISARLIPLARALPLSKLSVSAALAERIGSGPLLTRENLLGIEGDFAPGDPVVLVDP
                     AGLAIAVGEVLYAKAELFYRAGPLFRYHRVLV"
     gene            1612..2568
                     /locus_tag="AC16FU_06005"
     CDS             1612..2568
                     /locus_tag="AC16FU_06005"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR2993958.1"
                     /translation="MNIIHDTCQNEKKYFAASVVTLGTFDGVHRGHQELLRQITSRAG
                     KARLPAVLVTYHPHPVRVLRPAEAPPELTTLDEKLPLFELAGISDCIVMQFDQTLAHL
                     TALDFIERILVRKLGAREVTVGYDHRFGHQRTGTIDLLRQAGERWGFQVTVVEPVADG
                     SQPVKSSHIRQALQAGDMPTANLLLGHDFSLTGEIVPGHGVGKTMGYPTINLKLPPEK
                     IVPRYGIYASRVDLDGQVHYGLLYIGNRPTFPGALLSVEVYVLDYHNQERPARVVVSP
                     QVYLREDRKFPGPEALAAQMRKDEELVRSRFIPDTVKMTERR"
     gene            2593..2844
                     /gene="rpsO"
                     /locus_tag="AC16FU_06010"
     CDS             2593..2844
                     /gene="rpsO"
                     /locus_tag="AC16FU_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR2993959.1"
                     /translation="MKTETVRQYQKHPDDTGSPEVQIALLTQRINDISQHLAKSQKDF
                     HARRGLMRMVGQRRRLLDYLHATDVESYRKLIEKLDLRK"
     gene            2867..5053
                     /gene="pnp"
                     /locus_tag="AC16FU_06015"
     CDS             2867..5053
                     /gene="pnp"
                     /locus_tag="AC16FU_06015"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR2993960.1"
                     /translation="MIHSVEKEIGGKRLVIETGRVARQADGAVTVRLEDTVILATAVA
                     AREVTPDQDFFPLTVDYREKAYAAGKIPGGFFKREGRPREKEITSSRQIDRPLRPLFP
                     EGFFNEVQIMVSVLSSDQANDADVLGIIGASTALAISPIPFRTPVGAVRVGRIQGQLV
                     LNPTFAELDESDLNLVIAATTESIVMVEGGGREVGEADLVAALAFGHQAAKEIIALQE
                     ELQRQVGQPKWPVTPPAHNPELLAKVEQLAAERIRQANAIVDKQNRQAEIERITAEVK
                     TELAAAFPESEKQIAQILWDIEKREVRSLILDASRRVDRRKFDEIRPITCEVGYLPRT
                     HGSALFTRGQTQSLAVVTLGSKMDEQKLDELEGESYKSYMLHYNFPPFSVGEVRPNRG
                     PGRREIGHGVLAERAIQPVIPKEEQFPYTVRIVSDILESNGSSSMATVSSGSLALMDA
                     GVPIKAPVTGIAMGLVIEGGRYAILSDIQGVEDHLGDMDFKVAGTRQGITAFQLDIKV
                     PGITLEIMEKALRQAQAGRLFILEAMEKTIAQPRAALSPYAPRIIIFKIKPDKIGDVI
                     GPGGKTIRSIIEKTGAKIDIEDDGSVFISSVDQKAGELAQEMVLELVQEAEIGKVYTG
                     KVRRVTAFGAFVEIIPGQDGLVHISEIDTQRIARVEDVMNVGDMVTVKVIGIDEEGKI
                     RLSRKALLAPAAGDQEPSHERRHGHERSGHRSDRGSGPGRSHAHRR"
     gene            5060..>5385
                     /locus_tag="AC16FU_06020"
     CDS             5060..>5385
                     /locus_tag="AC16FU_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2993961.1"
                     /translation="MLQSLYKKTVLDSGLRIVTEYIPQVRSVSLGIWVNVGSRDERPQ
                     HSGISHFIEHMLFKGTKKRSAREIADALESVGGSINAFTSREQTCYYAKVLDEHLPVA
                     VEVLAD"
BASE COUNT         1156 a         1623 c         1647 g          959 t
ORIGIN      
        1 atcgggaaag taaggcatct tttagggccg acctgtccca tcatcaacat cgaccaccac
       61 ccggagaatg aacggttcgg tcaaatcaac tggctggata cgcgggcggc agcggtaggg
      121 gagatgattt acgaatttct ggtcgccgcc cggcacccga tttccccggc ggtggcgacc
      181 ctcctctaca cggcgattct caccgacacc ggccgcttcc gctacggcag cacccgcccg
      241 cagacccttt cgatttgcgc cgctctgatg gcgcagggcg ccgaccccaa ggtgatcacc
      301 gatgaggtct attttaacca cccccccgcc aatttgcgcc tgatcggaca ggtcgttcgc
      361 aatctggaac tggcctacgg gaagcgcgtc tgcgccctct ccatccgccg ggccgatctg
      421 gagcggttcg gagcgcactt tgaagatacc gagggtatcg tcgattattc ccttttcgcc
      481 aaaggcgtgg ccgtgggact tttgttcaag gaagtaaccc cgaggaaaac gaaggtcagt
      541 ctccgctccc agaaccacat cgatatcagc cgggtggcgc gtaaattcgg cggcggcggg
      601 cacgccaatg ccgcgggctg cagcgttgcg gccccgctgg agacagccaa aaagcaggtt
      661 ttgaaggaag tcatctggta cctccgcaat ggcgcaggac ggaatgctct taatcgataa
      721 accctcgggc cccacctccc acgacgtggt ggatcgggtg cgggccatcc tgaaagagaa
      781 gcgcgtaggc catactggca cgttggaccc cctggcctcc gggcttctgg tcatctgtgt
      841 cggccgggcc accaaagtcg cccgctacct tgcgggcgaa gacaaagaat accaggcggt
      901 gattcgtctc ggggtaacca cgacgaccca agaccgtacc ggagaaaaga tccagacccg
      961 acctgtggag ggcgtaaccc gggccgatgt tgaggccgcc ctcccgcaat tcatcgggtt
     1021 gcaaaagcaa accccgccgg ccttctcggc ggtgaaagtg caaggagtgc cggcctatcg
     1081 ttccgcccgg cggggtgagc cggtggcgct gaccccgcgt gaaatagaag tgttcgaatt
     1141 gcaattgacc gcttgcgaaa tgcctctggt cacgctccgc atccgctgct ccaaaggcac
     1201 ctacgtgcgc gcgctggcgg cggaaatcgg cgaacgtttg ggaaccggcg cgcatctggc
     1261 cgagctacga cgtacaaaag ttggaccttg ggacgtggcc gaagcgctca ccgttgacca
     1321 gttgcagcaa gccgctcagg agcaaaagat ctccgcccga ctgatcccgc tcgctcgagc
     1381 cttgcccttg tccaaactct ccgtctccgc cgcacttgcg gaaagaatcg gtagtgggcc
     1441 gctattgacg cgagaaaatc tcttgggaat tgagggggac ttcgctcccg gcgacccggt
     1501 ggtgctggtt gatccggccg ggctggccat tgccgttggc gaagtacttt atgcgaaagc
     1561 cgaactgttt tatcgggccg gaccgctttt tcgttatcac cgcgtactcg tatgaacatc
     1621 attcacgata cttgtcagaa cgaaaaaaaa tactttgcgg cctcggtggt taccctgggc
     1681 acttttgacg gcgtccaccg cggccaccag gaactgctcc ggcagataac gagccgggcc
     1741 gggaaagccc ggctccccgc cgtactggtc acctaccacc cccatccggt gcgggtcctc
     1801 cggccagccg aagccccacc ggaacttacc accctggacg aaaaactccc gctgttcgaa
     1861 ctggccggga tttcggactg tatcgtgatg cagttcgacc aaacgttggc ccacctcacg
     1921 gcacttgact ttatcgagcg tatccttgtt cgaaaattag gagcccggga ggtcaccgtc
     1981 ggctacgatc accgtttcgg ccaccagcgc accggaacca ttgacctgtt gcgccaggcc
     2041 ggcgagaggt ggggatttca ggtgacggtg gttgagccgg tagccgacgg gagccagccg
     2101 gtgaagtcga gccacatccg tcaagccctc caagccggag atatgccaac cgccaacctt
     2161 ctcttggggc acgatttctc cctcaccgga gaaatcgtac caggccacgg cgtggggaag
     2221 accatggggt acccgaccat caacctcaaa cttccgccag aaaaaatcgt tccaagatac
     2281 ggcatctacg ccagccgggt ggatctggac gggcaggtgc actacggatt gttgtatatt
     2341 ggcaaccggc cgactttccc cggtgctctc ctctcggtgg aagtctacgt cctggactat
     2401 cacaaccagg agcgtccggc tcgggtggta gtttccccgc aggtttacct ccgggaagat
     2461 cggaaatttc ccggtccgga agctctggcg gcacagatgc gtaaggatga agagctggtc
     2521 cggagccggt tcatcccgga tacggtgaaa atgacagaaa ggagatgagc ggaggtggag
     2581 aggcccaaag tgatgaaaac ggaaaccgtg cggcagtacc aaaagcaccc agacgacacc
     2641 ggctcgccgg aagtgcaaat tgcactgcta acgcagcgca tcaacgacat cagccagcat
     2701 ctggccaaaa gccagaaaga cttccacgcc cggcgtggct tgatgcgtat ggtcgggcaa
     2761 cggcgccggt tgttggatta cctgcacgcg accgatgtgg agagctaccg gaaactgatt
     2821 gagaaattgg acctgcggaa gtgagagaaa ggacaaagtc gagcgaatga tacattcagt
     2881 cgagaaggaa attggcggaa aacggctggt gatcgagacc gggcgggtgg cccggcaggc
     2941 cgacggagct gtcaccgttc ggctggaaga cacggtgatt ctggccaccg cggtggcggc
     3001 ccgggaggtg acgccggacc aagatttttt cccgctgacg gtggactacc gagagaaggc
     3061 ctacgcggcc gggaaaattc ccggcggctt cttcaagcgc gagggccggc cccgcgaaaa
     3121 agaaatcacc agctcgcggc aaatcgaccg gcccctgcgg ccgctcttcc cggaaggttt
     3181 tttcaacgaa gtgcagatca tggtctccgt gctctcgtcg gatcaggcca atgacgccga
     3241 tgttttggga atcatcggcg cctccacggc actggccatc tcccccatcc cctttcgcac
     3301 cccggtgggc gcggtccggg tgggccggat ccagggccag ctggtactga atccgacttt
     3361 tgccgagttg gacgagagcg atctcaactt ggtcatcgcc gccaccacag agagtattgt
     3421 gatggtggag gggggcggcc gggaagtggg cgaagccgac ctggtggcgg ctctggcctt
     3481 cggccaccag gcagccaaag aaattattgc ccttcaggaa gagctgcaaa ggcaggtggg
     3541 ccagcccaag tggcccgtta ccccgcccgc gcacaacccg gaattgctgg ccaaagttga
     3601 gcaattggct gcggaaagga tccgccaggc caacgccata gtcgacaaac aaaaccggca
     3661 ggcggaaatc gagcgtatca ccgccgaggt aaagaccgaa ttggcagccg cgtttccgga
     3721 atcagaaaag cagatcgccc agatcctctg ggatattgaa aagcgtgagg tccgctccct
     3781 cattctggat gccagccggc gggtcgaccg gcggaagttt gacgaaatcc ggcccattac
     3841 ctgcgaagtc ggctacctgc cccgcaccca cggctcggct ctgttcaccc ggggtcagac
     3901 gcagagtctc gcggtcgtga ctctgggctc caagatggat gagcagaaat tagacgaact
     3961 cgagggggaa tcgtacaaat cgtacatgct ccactacaac ttcccgccct tctcggtggg
     4021 cgaagtccgg ccgaaccggg gaccgggccg gcgggaaatc ggccacggcg ttttagctga
     4081 gcgggccatt caaccggtca ttcccaaaga ggagcaattc ccgtacacgg tccgcatcgt
     4141 ctcagacatc ctggagtcca acggctcttc atcgatggcc acggtctcca gcggcagcct
     4201 ggccttgatg gatgccgggg tcccgatcaa ggcaccggtg acgggcattg cgatgggcct
     4261 ggtgatcgaa ggcggccgtt atgcgatcct ctccgacatt cagggggtgg aggaccacct
     4321 gggggatatg gacttcaaag ttgccggcac ccggcagggc atcaccgcct tccagctgga
     4381 cattaaggtc ccgggaatca ccctggagat tatggaaaaa gcgctccggc aggcgcaggc
     4441 cgggcgcctc ttcattctcg aggcgatgga aaaaactatt gcccagccgc gcgctgcgct
     4501 ttcgccctat gcgccgcgga tcataatctt caagatcaaa ccggataaga tcggcgacgt
     4561 catcgggccg ggcggcaaga cgatcagaag catcatcgag aagaccggcg ccaaaatcga
     4621 tattgaagac gacggctcgg ttttcatctc ctcggttgac caaaaagcgg gcgaactggc
     4681 ccaagagatg gtgctggaac tggtgcagga agccgaaatc ggcaaggtct acaccggcaa
     4741 ggtccggcgg gtaaccgcct tcggcgcctt tgtggaaatc atcccgggac aggatgggtt
     4801 agtccacatt tccgaaatcg acacccagcg catagcccgg gtagaggacg tgatgaacgt
     4861 gggcgacatg gtgaccgtga aagtgatcgg catcgacgaa gaagggaaga ttcgcctttc
     4921 gcgcaaggcg cttttggcac ccgccgcggg tgatcaggaa ccgtcccacg agaggcggca
     4981 tgggcacgaa cggtcgggcc accggtccga ccggggttcg ggcccgggcc gttcccacgc
     5041 gcacagacgg taggtcggga tgttgcagtc gctctacaag aaaaccgtcc tggactccgg
     5101 tctgcgcatc gtcaccgaat acatcccgca ggtgcgctcg gtttccctgg gaatctgggt
     5161 gaacgtgggt tcccgtgacg agcgtccgca gcactccggc atctcgcact tcatcgagca
     5221 catgctcttc aaagggacga aaaaacgcag cgcccgggaa atcgccgacg cgctggaatc
     5281 ggtgggcggc tccatcaacg ccttcacctc gcgggagcag acctgctact acgccaaggt
     5341 actggacgaa cacctgccgg tcgccgtcga agtcctggcc gacat
//
LOCUS       DBNGZA010000282         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_898484, whole genome shotgun sequence.
ACCESSION   DBNGZA010000282 DBNGZA010000000
VERSION     DBNGZA010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_898484"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2084
                     /locus_tag="AC16FU_06025"
     CDS             <1..>2084
                     /locus_tag="AC16FU_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR2993962.1"
                     /translation="LGLSVGCIQNEMDPAERRTQYSCDITYGTNNEFGFDYLRDNMAV
                     RLEDRVQRGFRYAIVDEVDSVLIDEARTPLIISGPTNITTAEYGEMQPLVEQVVRKQT
                     VLVNRLVAEAEELLKNDGEYDAGTKLLAARRGAPKNKRLFKLLQEEGVKKLVSRVESD
                     YMRDKKLHEIDDLLYFFIDEKEHTAELNEMGREMLSPEDQKLFVIPDIVEGLHQIEAD
                     EALSPEEKIKKKEELYRLHAERSQKVHATSQLIKAYSLYERDVEYVVQDGKIIIVDEF
                     TGRLMPGRRYSDGMHQAIEAKEGVRVEQETQTLATITLQNFFRMYDKLAGMTGTAVTE
                     ANEFFQIYKLDVLSIPTNEPVRRIDYDDQVYRSRREKYNAIIEEITQYHNENRPVLVG
                     TTSVEVSETLSRMLKRQGIRHNVLNAKFHQQEAEIITQAGQAGAVTIATNMAGRGTDI
                     KLGEGVVKHPNCALVELKPDVEPCPYLKELNCYERVPCGLHILGTERHEARRIDRQLR
                     GRSGRQGDPGSSRFYLSLEDDLMRLFGSERIASIMDRLGLEEGEVIQHRMVTKAIERA
                     QKRVEAQNFAIRKHLLEYDDVMNQQREVIYDRRLAALEKEDLQEEVLALIDQTVEEII
                     NRHGGQERYVEDWDWTGLREEFRRVFLVEVRFKPEEFAKLTYPQIIEAYKNAALEVYR
                     AKEATVGPEVMR"
BASE COUNT          504 a          585 c          653 g          342 t
ORIGIN      
        1 ctcggccttt ccgtcggctg catccagaac gaaatggacc cggcggagcg gcgcacccag
       61 tacagctgcg acatcaccta cgggacgaat aacgaattcg ggttcgacta cctgcgcgac
      121 aacatggcgg tgcggctgga agaccgggtg cagcgcggct tccgctacgc catcgtggac
      181 gaagtcgatt cggtcttaat cgacgaggcg cggacgcctt tgatcatctc cggccccacc
      241 aacatcacca ccgccgaata cggcgagatg cagcccttgg tggaacaggt ggtgcggaag
      301 cagacggtgc tggtgaacag attagtggcc gaggcggaag aactgctgaa gaacgacggc
      361 gaatatgatg ccggcaccaa gcttttggcg gcgcggcggg gcgcgcccaa gaataaacgc
      421 ctgttcaaac ttttgcagga agaaggggtg aagaaattag tctcgcgcgt ggaatccgat
      481 tacatgcgcg acaaaaagct ccacgagatc gacgacctgc tctacttttt catcgacgaa
      541 aaggagcaca ccgccgaatt gaacgaaatg ggccgggaga tgctctcccc ggaagaccag
      601 aagctcttcg tcatcccgga catcgtggag gggctgcacc agattgaagc ggacgaagcg
      661 ctttccccgg aagagaaaat caaaaagaaa gaagagctct accggctgca cgcggaaagg
      721 tcgcagaaag tccacgccac gagccagttg atcaaggcct actccctcta cgagcgggac
      781 gtggagtacg tggtgcagga cggcaaaatc atcatcgtgg atgaattcac cggccgcttg
      841 atgccgggcc ggcgctactc cgacgggatg caccaggcga ttgaggccaa agaaggggtc
      901 agggtcgagc aggagacgca gacactcgcc acgatcaccc tgcagaactt cttccggatg
      961 tacgacaagc tggccgggat gaccggcacc gctgtgaccg aagcgaacga gttcttccag
     1021 atttacaagt tggacgtcct ttccatcccc accaacgagc cggtgcggcg gatcgactac
     1081 gacgatcagg tttaccgcag ccggcgcgaa aagtacaacg ccatcatcga agagatcacc
     1141 cagtaccaca acgagaaccg cccggtattg gtcggtacca cctcggtcga agtttccgaa
     1201 accctttccc ggatgctcaa gcggcagggg atccggcaca acgttttgaa cgccaagttc
     1261 caccagcagg aggcggaaat catcactcag gccggacagg cgggcgcggt gaccatagcc
     1321 acgaacatgg cggggcgcgg caccgacatc aaattggggg agggtgtggt caaacacccc
     1381 aactgcgcgt tggtggaact caaaccggac gtggagccgt gtccctatct caaggaattg
     1441 aactgctacg agcgcgtccc ctgcggcctg cacatcttgg gcaccgaaag gcacgaggcg
     1501 cggcggattg accggcagtt gcggggccgt tccgggcgtc agggcgatcc cggctcctcc
     1561 cgcttctacc tttctttgga agacgacctg atgcgccttt tcggctcgga gcggatcgcc
     1621 tcgattatgg atcgcttggg cctcgaagaa ggggaagtga tccagcaccg gatggtgacc
     1681 aaagcgatcg agcgggcgca aaagcgggtg gaagcgcaga acttcgccat ccgcaagcac
     1741 cttttggagt acgacgacgt gatgaaccag cagcgggagg tgatctacga ccggcgcctg
     1801 gcggcgttgg aaaaagaaga cttgcaagag gaagtgctgg cgctcatcga ccagacggtc
     1861 gaggaaatca tcaaccgcca cggcgggcag gaaaggtacg tcgaggactg ggattggacc
     1921 gggctgcgcg aagagttccg ccgggtcttt ttggtcgaag tccgctttaa gccggaagaa
     1981 tttgcgaaac tgacctaccc gcaaattatc gaggcctaca aaaatgcggc gttggaggtc
     2041 taccgggcca aagaagccac ggtggggccg gaggtaatgc gcaa
//
LOCUS       DBNGZA010000283         8072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1430996, whole genome shotgun sequence.
ACCESSION   DBNGZA010000283 DBNGZA010000000
VERSION     DBNGZA010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1430996"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16FU_06030"
     CDS             complement(<1..313)
                     /locus_tag="AC16FU_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR2993963.1"
                     /translation="MKKTVAIILAAGKGTRMKSPLPKVLHRVGHRPMVWHVIQAARKA
                     DIREIIVVVGFQALRVERALANTDVEFVHQEPQLGTGHAVMVARPLLTHFHGTILVLA
                     GD"
     gene            complement(315..671)
                     /gene="panD"
                     /locus_tag="AC16FU_06035"
     CDS             complement(315..671)
                     /gene="panD"
                     /locus_tag="AC16FU_06035"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR2993964.1"
                     /translation="MLLECCKAKIHHAAVTEANLRYEGSITIDGSILKAAGILPFERV
                     QVVNVNNGERFDTYVIAGRANSRVICLNGPAARLGQVGDRVIIIAYCLLEQDEAKRHQ
                     PRIVILDDRNRIKKRN"
     gene            complement(684..1535)
                     /gene="panC"
                     /locus_tag="AC16FU_06040"
     CDS             complement(684..1535)
                     /gene="panC"
                     /locus_tag="AC16FU_06040"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR2993965.1"
                     /translation="MKIITSVRQMQNVSLALKEKRRSIAFVPTMGYLHAGHLSLIRRA
                     ASLGDVVVVSIFVNPAQFGPADDFARYPRDLKRDTDLAKTAGCDILFVPATEKIYPPD
                     FQTCVQVERVSRGLEGERRPGHFRGVATVVAKLFNIVQPDIAIFGQKDAQQAVVIKQM
                     VRDLNLPVKIVVAPTVREKEGLAHSSRNVYLTPEERKQAPVLYQSLREAKKLVLQGER
                     NVPKIQTQITEMIRSQSGAEIEYVALTDAPTLEPIQNLSGKVLISLAVRFGKTRLIDN
                     IAVTIPK"
     gene            complement(1558..2397)
                     /gene="panB"
                     /locus_tag="AC16FU_06045"
     CDS             complement(1558..2397)
                     /gene="panB"
                     /locus_tag="AC16FU_06045"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR2993966.1"
                     /translation="MATLVSPQVKRPMQPERVTTRTFRQLKEKGEKIALLTAYDFFTA
                     KILDEVGCDAILVGDSANMVFYGAPNTLSMTMEQMLYHTRAVSLAAKRAMVIGDMPFL
                     SYQPSAEVAVTNAGRFLKEGGAQAVKLEGGSEMAEITRKVVEAGIPVMGHIGLVPQAI
                     HRTGGYAVQGKDAASQEYLQESARALEKAGCFSLVLEGIKSDVAKKITEAVAIPTIGI
                     GAGPHCDGQVLVVNDILGLYETFKPKFVRQYANLAVEIRGACQKFKDDVKSKNFPSSD
                     ESY"
     gene            complement(2424..3383)
                     /locus_tag="AC16FU_06050"
     CDS             complement(2424..3383)
                     /locus_tag="AC16FU_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993967.1"
                     /translation="MSIRNRSLAALAVVLLVAAQSQADVKITSKMTMSAGAAAMSMSG
                     MQTMLVKGDKEKMIMGFSLPQATQQNTPGQGEMEMAMIMRLDKQVLWMVMPMMKTYSE
                     MSFPDLKKMQAQAMQRMQGDSAAQVMKKVLGGMKLQKSTETKKVLGYPAQKYTLSMGG
                     ESDTTGLFQGMRFEMTLWVSNQFPGWKELEACQKKSMELMGGEENFLSTLNPMFSSFG
                     GGLGMDEFMQFAKGANGYPLAMDMQMYARGGQSQNWQDAMKKLGPKEKPAAPDTTAKE
                     QLVMSVSMEATAISQKPIADSEFDPPAGYQKGQFPGAFQGEEK"
     gene            complement(3383..3928)
                     /gene="folK"
                     /locus_tag="AC16FU_06055"
     CDS             complement(3383..3928)
                     /gene="folK"
                     /locus_tag="AC16FU_06055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR2993968.1"
                     /translation="MSTAFVGLGSNLGDRLHNLKAAWQELSWLPGTKAVTASSLYETE
                     PVEFADQPYFLNQVVQLETTLDPEELLEELLRIEAHLGRTRTVPKGPRTLDLDLLLFD
                     QLIIETPRLTLPHPALHRRRFVLVPLAEIAPAVLHPILRKKMVALLAELSDQSSVQMY
                     RKREAATAATLSTAVPSSKEG"
     gene            complement(3931..4290)
                     /gene="folB"
                     /locus_tag="AC16FU_06060"
     CDS             complement(3931..4290)
                     /gene="folB"
                     /locus_tag="AC16FU_06060"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR2993969.1"
                     /translation="MDVIRLHNMTFYGYHGASSAERETGRRFEVDAELFFPVSRAART
                     DRLKNTIDYTKVYTCVEKVILQNHFTLLETVADRLAQSILKNFPIRKVTIRVRKKIPP
                     IPGNLDHIEVELTRSKR"
     gene            complement(4294..5550)
                     /locus_tag="AC16FU_06065"
     CDS             complement(4294..5550)
                     /locus_tag="AC16FU_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2993970.1"
                     /translation="MIKRVILDKAERLQQLTPDFTYAVAPARARMGKRLVVPQIDLSS
                     ELSDLPPPASVTALLERSPDILMKRRPAEPKTTAEFKGCIAEWFSERYGVTLDPKKEI
                     LPTLGNRAALFGLALAYLNPKEKAALPNPGYPFYRLAVLTAGGLAEYLPLRERNDYLP
                     TLAQLESESPSLRHRLRGQETLRQPKFVFLNYPNNPTGAVADEQFYQNAVRFASDHNV
                     LLINDAVYSELTYDFRPQSLLSPDHAKRVAVEFHSFTFTFGLSRIPLGFAVGNRDVLA
                     TLEALFHTLGSTPDEFSLQIGLAALESATGSIDHLRRTFRARRDLALTRVTSFGWQVR
                     KPLGGPFIWIKLPRSFSSVGFARRILRKAGVVVTPGVYFGEQGEGYVRLSLCKPEAEL
                     TRAFDRIAQTWPLLRPSLKSKTAASV"
     gene            complement(5563..6159)
                     /gene="pyrE"
                     /locus_tag="AC16FU_06070"
     CDS             complement(5563..6159)
                     /gene="pyrE"
                     /locus_tag="AC16FU_06070"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR2993971.1"
                     /translation="MTPTDVLEISKQAGALLTGHFILTSGRHSDVYFEKFAVLQHPAY
                     LEKLAGELARCFDSEKIDLVVGPTTGGMLLAYEVARQWGKRFLFAEPGENGRVFKRGF
                     TLAPGTRVLVVDDVLTTGRSVAEVLALVRSYGATVAGIGLLLDRSEKPLDFGIAYTAL
                     TKVTATSFVPDACPLCQKGLPLTKPGSREQPAKSTAAS"
     gene            complement(6184..6573)
                     /gene="acpS"
                     /locus_tag="AC16FU_06075"
     CDS             complement(6184..6573)
                     /gene="acpS"
                     /locus_tag="AC16FU_06075"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR2993972.1"
                     /translation="MLPPDMVAGVGVDIIEVSRIQAAIERWGERFLKRIYTPGEVAYC
                     RSLERPFESFAGRFAVKEACMKALGTGWTDGVKWRSFEVKNEKSGKPYVVLDGDLDKR
                     INGKKILVSISHTENYATGVAVLSDQP"
     gene            complement(6883..7614)
                     /locus_tag="AC16FU_06080"
     CDS             complement(6883..7614)
                     /locus_tag="AC16FU_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993973.1"
                     /translation="MKELLQDIKTVTGVSGVMILDKRREATHQLLPASLAPEQVKKLA
                     MKLVIVANSIERPGRIDLRFTEGWTILVNLPSGAVLVLTKDSVDFSMLRLVLKKVLAQ
                     LAREVDAPSTQTAHSGAALDSRSIETLFEVTNSIAGRYREVQGPFQVLQHLRKAKEKL
                     LPSHPGLVNFFIDPNGQLLFGRLKEGSVELTVDTFALFLAQFLNYANGPGNKLPPGTL
                     TEWLWEYRERLEGLNFFHALHAVKS"
     gene            complement(7611..>8072)
                     /locus_tag="AC16FU_06085"
     CDS             complement(7611..>8072)
                     /locus_tag="AC16FU_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993974.1"
                     /translation="QADLDLAIQKQHADTVAKIEVECEQLWAQVGKNPPATQKVPLAV
                     ATAQLEEKLKTTPGLVGLIVASYEGKVLNQAELAGPTKSWATLVAELAGFAGLLAKAT
                     RLGSLVEGTAEFPEGKLVVSGQPETVVGIVVKTTTDTQQAKDNLKLALQER"
BASE COUNT         1595 a         2316 c         2362 g         1799 t
ORIGIN      
        1 cgtcgcccgc caacaccaaa atcgtcccgt gaaagtgggt caagagcggc cgggccacca
       61 tgacggcgtg gccggtgccc agctgcggct cctggtggac gaattcgacg tcggtattgg
      121 ccagcgcccg ttcgacgcgg agcgcctgaa agcctaccac gacgatgatt tccctgatat
      181 ctgccttgcg cgcggcctga atgacgtgcc agaccatagg ccggtgcccc acccggtgga
      241 gcaccttggg caagggcgac ttcatccggg tgcctttgcc agcggccagg attatggcga
      301 cggttttctt catctcagtt tcgcttcttg attcggttgc ggtcgtctaa aatcacgatg
      361 cgcggctggt gtcttttcgc ttcgtcctgt tccagcaggc agtaggcaat gataatcacc
      421 cggtcaccca cctggccgag acgggcggcc ggcccgttca gacagatgac ccgggagttg
      481 gcccggccgg cgatgacgta ggtatcaaac cgctcgccgt tgtttacgtt aaccacctgc
      541 acccgctcga agggcagaat gcccgccgct ttcaggattg agccatcaat ggtgatactc
      601 ccctcgtacc ggaggtttgc ttcggtaacg gcggcgtggt ggattttggc cttacaacat
      661 tctaaaagca tcgtcttttg atctcacttc ggtatcgtga ccgcaatatt atcaatcaac
      721 cgcgttttcc caaagcggac ggcaagcgaa attaatactt ttcccgagag attttgtatg
      781 ggttccagcg taggggcgtc ggtgagcgcc acgtattcga tttccgctcc ggattgtgag
      841 cgaatcattt cagttatttg cgtctgtatt ttaggcacgt tgcgctcacc ttgcagaacg
      901 agttttttcg cttcccgaag cgactggtag agcaccggcg cctgcttccg ctcctccggg
      961 gtgaggtaaa cgttgcgcga actatgagcc aggccttctt tttcgcggac ggtcggggcg
     1021 acgacgattt tcaccggcag gtttaagtcc ctcaccattt gcttgatcac caccgcctgc
     1081 tgggcgtctt tctgtccaaa aatggcaata tccggctgga cgatattaaa caacttcgcc
     1141 accaccgttg ccacgcctct gaagtgcccc ggacgcctct cgccttcgag acctcgcgac
     1201 actctctcaa cttggacgca ggtttgaaaa tcgggaggat agatcttctc ggttgcggga
     1261 acaaaaagaa tatcacaacc ggcagttttc gccaggtcgg tgtcccgctt taaatcacgc
     1321 gggtagcggg cgaaatcatc agccgggccg aactgggcgg gattgacaaa aatcgagact
     1381 acgacgacat cgcccagact ggcggcacgg cgaatcaggc tgagatgtcc tgcgtgcagg
     1441 tagcccatcg tcggtacgaa agcaatcgac cggcgctttt ctttaagcgc cagtgacaca
     1501 ttttgcatct gacggactga agtaatgatt ttcatggaag gaaaaaacct ggaggggtta
     1561 gtagctctcg tccgaggagg gaaagttttt ggatttcacg tcgtccttga acttctggca
     1621 ggcaccgcgg atttccacag cgagattggc gtactgccgg acgaatttgg gtttgaacgt
     1681 ttcgtacagt cccaaaatat cgttcaccac taaaacctgc ccgtcgcagt gaggcccggc
     1741 gccgatgccg atggtaggaa ttgccaccgc ctccgtgatt tttttggcca cgtcactttt
     1801 tattccctca aggactaaag aaaagcaacc cgctttttcc agtgcccgcg cactctcctg
     1861 caaatactcc tgcgaggccg catctttgcc ctgaaccgcg taaccgccgg tgcggtggat
     1921 cgcctgcggc accaggccga tgtgccccat caccggaatc ccggcttcca ccaccttgcg
     1981 ggtgatctcc gccatttcac tgccgccttc cagtttcacc gcctgggcgc cgccttcctt
     2041 cagaaagcgg cccgcattgg ttaccgccac ttcggcggag ggctggtacg aaagaaaggg
     2101 catatcgccg atgaccatcg cccgcttggc cgccagactg accgcgcggg tgtggtagag
     2161 catctgctcc atcgtcatgg agagggtgtt cggcgcgccg tagaagacca tattggcgga
     2221 gtcgccgacc agaatcgcat cgcagccgac ttcgtccaag attttggccg tgaagaagtc
     2281 gtaggcggta agaagggcga tcttctcgcc cttctctttc aactgccgga aggtgcgggt
     2341 ggtgacccgt tcgggctgca ttgggcgttt gacctgaggc gacacgagtg tggccataaa
     2401 agcgtcaggt ggctatccgc gacttacttc tcttcgcctt gaaaagcgcc cgggaactgg
     2461 cctttctggt agccggccgg cgggtcgaat tcgctgtcgg ctatcggctt ctggctgatc
     2521 gccgtcgctt ccatagacac cgacattacc agttgctctt tggcggtggt gtccggtgcg
     2581 gctggctttt ccttcggtcc cagttttttc atcgcgtcct gccagttctg ggattggccg
     2641 ccgcgggcgt acatctgcat atccatggcc agggggtagc cattggctcc tttggcaaat
     2701 tgcatgaatt catccatccc cagaccgccc ccaaaggacg agaacatggg gttcagcgtt
     2761 gagagaaaat tttcctcacc gcccatgagt tccatcgatt tcttctggca ggcttccagt
     2821 tctttccagc cggggaactg gttggaaacc cacagggtca tctcgaaacg catcccctgg
     2881 aacaggccgg tcgtgtcgct ctccccgccc atactgagcg tgtacttctg cgccggatag
     2941 cccaaaacct ttttcgtttc ggtggatttc tgcagtttca tcccccctaa tacctttttc
     3001 atcacctggg cggccgaatc gccctgcata cgctgcattg cctgggcctg catcttcttc
     3061 aggtcgggaa acgacatttc actgtaggtt ttcatcatcg gcatgaccat ccacagcacc
     3121 tgcttgtcca ggcgcataat catggccatc tccatctcac cctggccggg cgtgttttgc
     3181 tgagtggcct ggggcagaga gaagcccatg atcattttct ctttgtcacc tttcactaac
     3241 atggtctgca tgccggacat cgacatcgcc gcggcgccgg ccgacatggt catcttggag
     3301 gtgattttca catccgcctg actttgcgcg gcgacgagca ggaccacggc gagtgcggcg
     3361 agacttcggt ttcggatcga cattacccct cctttgagct gggaactgcg gttgacaatg
     3421 ttgccgctgt tgcggcttct ctcttccggt acatctgtac gctcgattga tcggaaagtt
     3481 cggccaaaag cgcgaccatt ttttttctca atatcgggtg gagaacggcg ggcgcaattt
     3541 cagccaacgg cacaagtaca aaccgtctcc ggtgcaaggc cggatgggga agggtcaggc
     3601 ggggtgtctc gataatcagt tgatcgaaca agagcaggtc gaggtcaagc gtgcgcgggc
     3661 ctttgggtac ggtgcgggtt cggcccaggt gcgcttcaat gcgtaaaagt tcttccagca
     3721 gctcttcggg atctaaagtt gtctccagtt gcaccacctg gttcaagaaa tagggctggt
     3781 cggcaaattc taccggctcg gtttcataaa gagaagaggc cgtgaccgcc ttcgttcccg
     3841 gcaaccaaga gagttcctgc caggccgctt ttaggttatg caaccgatcg ccgagatttg
     3901 atcctaaacc gacaaaggcc gtgctcactt ttacctcttg ctccgggtca gttccacctc
     3961 gatgtgatct aagttgcccg gaatgggcgg aatttttttc cgcacccgga tggtcacctt
     4021 ccggatggga aagtttttca gaatggactg cgccaggcga tcggccaccg tttccagcag
     4081 cgtgaaatgg ttttgcagga taaccttctc cacgcaggta tagaccttgg tataatcaat
     4141 ggtgttcttc agccggtcgg tgcgggcggc ccggctgacc gggaagaaca gttcggcatc
     4201 gacctcgaag cggcggccgg tctccctttc ggcggacgag gcgccgtggt agccgtagaa
     4261 ggtcatgttg tgcaggcgga tcacatccat cgttcagacc gaggccgcgg ttttgctctt
     4321 caacgacggc cgcagcaacg gccaggtttg ggcgatgcga tcaaaggccc gggtcaattc
     4381 cgcttcgggc ttgcacaggg acaggcgcac gtagccttca ccctgctcgc cgaaatatac
     4441 gccgggggtc acgaccacac cagccttgcg caggatccgt cgcgcaaagc ccaccgatga
     4501 aaagctgcgc ggaagtttga tccagatgaa tggcccgccc agaggttttc tgacctgcca
     4561 accgaaagac gttacgcggg taagagcaag atcccgccgc gcgcggaagg tgcggcgcag
     4621 gtgatcgatc gacccggtgg ccgattccag cgcggcgagg ccgatctgga gcgaaaactc
     4681 atcgggggtg ctccccagcg tgtgaaagag cgcctccagc gtggccaaga cgtcgcggtt
     4741 ccccacggca aagcccagcg ggatgcggct tagaccaaag gtaaaggtga agctgtggaa
     4801 ttcaactgcc acgcgtttgg catggtccgg cgacaaaaga ctttgcggcc ggaagtcgta
     4861 cgtcagttcg gagtaaacgg catcattgat gagcaaaaca ttgtgatccg aagcgaagcg
     4921 aaccgcattc tggtagaact gctcatcggc gactgccccg gtgggattgt tcgggtagtt
     4981 caaaaacaca aatttcggct ggcggagtgt ttcctggccg cgcaggcggt gccgcaggct
     5041 gggggactca ctttccagtt gcgccagtgt ggggaggtaa tcgttccgct cacgcagagg
     5101 caggtactcg gccagaccgc cagcggtcag aacggcgagc cggtaaaatg gataccctgg
     5161 attcggcagg gccgcttttt ctttcgggtt gagataagct aaagccaggc cgaaaagagc
     5221 ggcccggttt cccagcgtgg gcaggatttc ctttttcggg tcgagggtca cgccgtaacg
     5281 ctcggaaaac cactccgcga tgcacccctt gaattccgcc gtggtcttcg gctctgcggg
     5341 acggcgtttc atcaggatgt cgggtgaccg ctctaaaagc gccgtgacgg aagccggcgg
     5401 cggcaggtcg gagagttcgg aacttaagtc gatctggggg acgaccagcc gctttcccat
     5461 ccgggccctg gccggtgcca ccgcgtaggt gaagtcgggg gtcaactgct gcaaacgttc
     5521 cgctttgtct aaaatgacgc gtttgatcat ggggctgcta attcatgagg cagcagtact
     5581 cttcgccggt tgttcccggc tgcccggttt ggtgaggggt agacccttct ggcagagggg
     5641 acaggcgtcc ggcacaaatg aggtggcagt cacttttgtc aaagccgtgt acgcgatgcc
     5701 gaaatcgagc ggtttttcgg agcggtcgag taagaggcct attccggcga cggtcgctcc
     5761 atacgatctg accaatgcca gcacttcggc caccgagcgg ccggtggtca aaacgtcatc
     5821 cacgaccagc acccgggtgc cgggggccaa agtaaagccc cttttgaaga cccggccgtt
     5881 ttcaccgggt tcggcaaaga ggaagcgttt cccccactgc cgggcaacct cgtacgccag
     5941 aagcattccg ccggtggtgg ggccgaccac caggtcaatt ttttccgaat cgaagcaccg
     6001 ggcgagttct ccggccagct tttccagata agccggatgt tgcagcaccg cgaatttctc
     6061 aaagtagacg tcgctgtgcc ggccggaggt cagaataaag tggccggtga gcagcgctcc
     6121 cgcctgctta gagatttcga ggacatcagt tggagtcacg atagtcccaa gtctttcatt
     6181 agcttaaggt tggtcgctta gcaccgccac gccggtggcg taattttcgg tgtgggagat
     6241 cgatacgagt atcttctttc catttatcct tttgtcgaga tcaccgtcca gcaccacgta
     6301 cggcttcccc gatttttcgt ttttcacctc aaacgaccgc catttcaccc catcggtcca
     6361 gccggtgcct aaggccttca tacaggcctc cttgaccgcg aaccggccgg cgaacgactc
     6421 aaacggccgc tccaagctcc ggcaataagc cacttcgccc ggggtgtaaa tccgcttcag
     6481 gaacctttcg ccccagcgct ctatggccgc ctgaatgcgg gatacctcaa taatatccac
     6541 accgacgccg gccaccatat ccggtggtag catacgggaa tcgttggcgg ttggcaatcc
     6601 gggtggtata ttaagcccga cgtgggagga gataatttga aaaacctctt acaaggataa
     6661 gatgaaggcc gctcccctga gttgcgcgta cgcgatgact tcccgcaagg gaaagagcga
     6721 aagcaaaaga cgaagggtgg ctgtaaaacg cctttaccgt tctatttgag agggtttttc
     6781 aaatagagca ctcttccccg gcaaagtcct cccgcctcgg gcgggaggct tttttattgc
     6841 tcctcagagt cggatctgcc tgagggggat aaggtccgag tcttacgact tgaccgcgtg
     6901 gagggcgtgg aagaagttga gcccctccag ccgttcgcgg tactcccaga gccattcggt
     6961 gagggtgccc ggagggagct tattgcccgg cccgttggcg tagttcaaga actgggccaa
     7021 aaagagcgcg aaggtgtcta cggtcagttc taccgagccc tcttttaagc ggccgaagag
     7081 gagttgaccg ttggggtcga tgaaaaagtt caccaagccg ggatgggagg ggaggagttt
     7141 ttctttggct ttgcgcaaat gctggagcac ctggaacggc ccctgcacct cccggtagcg
     7201 gccggcaatg gagttcgtga cttcaaagag ggtttcaatg cttcgggaat cgagcgccgc
     7261 cccggagtgc gctgtctgcg ttgagggagc gtcaacttcc cgcgccagct gcgccagaac
     7321 cttcttcagc accagccgga gcatcgaaaa atcgacgctg tccttggtga gcacgagcac
     7381 agcgccggaa gggaggttca ctaaaatcgt ccagccctca gtgaagcgga ggtcaatccg
     7441 gccgggcctt tcgatgctgt tggccacgat gaccagtttc atagccagtt tcttcacctg
     7501 ctccggcgcc agtgaagcgg gcagaagctg gtgggtggct tcgcggcgct tgtccagaat
     7561 catgacgccg gaaaccccgg tgacggtctt gatatcctgc aggagttctt tcatcgctct
     7621 tggagcgcca gtttcaggtt gtccttggcc tgctgggtgt cggtggtggt cttgacgacg
     7681 atgcccacca ccgtttccgg ctggccggac acgactaatt tgccctccgg gaactcggcg
     7741 gtaccttcaa ccagactccc cagccgggtc gctttggcca ggagcccggc aaaaccggcc
     7801 agttcagcca ccagggtcgc ccacgacttg gtcgggccgg cgagttcggc ctgattcaaa
     7861 accttgcctt cgtacgaggc cacgatcagg cccaccagtc cgggagtggt cttcagtttt
     7921 tcttcgagct gggcggtggc gacggccaga ggaacttttt gcgtggcggg aggatttttc
     7981 cccacctggg cccagagctg ttcgcattcg acttcaattt tcgctaccgt gtcggcgtgc
     8041 tgtttctgga tggctaaatc gagatcggcc tg
//
LOCUS       DBNGZA010000284         6450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1111529, whole genome shotgun sequence.
ACCESSION   DBNGZA010000284 DBNGZA010000000
VERSION     DBNGZA010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1111529"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1399)
                     /locus_tag="AC16FU_06090"
     CDS             complement(47..1399)
                     /locus_tag="AC16FU_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993975.1"
                     /translation="MRVRIFLFTVVIGLLAASVFALVPSVPNLINFQGRLTDASGVPV
                     ADGVHAVTFRIYSTAVGGVLLWDDPVAGGVTTSGGLFTYELGSDPADLLPVDLFQDNN
                     EAWLEVEADAVILSPRTRLISTPYTRVAGSLEVPSAFVPGVVGIATFGNHQLSTYGSD
                     GQEQIRLWGASYGEILLHDADPTNDETVRLTANASSGGELELRDNGGLTTIDLHGGLT
                     GDASAILPTDAINSLEIKDEPGIAHSFTFGSTILPVAPGTIPIESVTITYPANGYVVV
                     TAHGFFKLTHVTGTDTWGRAFISTVRGGIDFDNFALFTVESSHPSFSGDKGYRSFSLT
                     KVEAVTGGTTVKYYLNADRWTPNAIGSLSEINRVHLLAQYFPTAYGTVVSTVASVPSG
                     LEPSAMAGVPGVGSQQSEVRTITVEEHNARLKAEVAEMKARLQTLEEQVKQQNAGGGR
                     "
     gene            complement(1652..3847)
                     /locus_tag="AC16FU_06095"
     CDS             complement(1652..3847)
                     /locus_tag="AC16FU_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993976.1"
                     /translation="MRSLVRLGRRSLVFGFLTLTFSLFFIPIPQAAARESSQGSVVVS
                     AFILDWPFVLKYKKEGLARNDNLGTAVAGAGDVNNDGYGDFIVGAPFTDPNGLTDAGS
                     AFVYSGRDGALLFQKDGGAAGDEFGLAVSGAGDVNNDGYDDFIVGAPLADPGALADAG
                     SAYVYSGLNGTLLYQKNGNASGQQLGHAVASAGHVNADAFADFIVGVHAASPNLITSA
                     GIVFVFSGADGSVLHQKNGTELAGQLGYSVAGLGDINGDGFDDFAAGANRASPGFRSN
                     AGSVFVYSGSGGGLLFQKDGTAAGDQLGYSTAGGGDVNNDGVPDLAAGAPGANPGGLN
                     DAGSAYVFSGATGGVLYQKNGIDVSNQFGLSVAIVGDATHDGFAEFLAGAPLADPGGR
                     GFAGTAVLFSGATGDTLLWRAGASAGDQLGFAVAALGDLNADGYADFAIGAPFTDPAD
                     FNLGTVLAFLSGYEMTLTSVANVPDDQGRQFQLNWKSRPTGSGLGQKFQIFRRDSPPF
                     GAVATPPGTWSKIKEVPVVVTESLYTPTVQTPTNREVFFVRNVANDSTVFFDSNPDSG
                     VATDELFPPAPAVTVASTLPCSALVVSWHPVAAPDLEHYEVFRDTIFNLATKIFLANV
                     GRDTVYVDRTALPNQFYFYLVVAQDSNGNRSFSVASGANLQTGDVDGDGLVDIIDVIG
                     LINDVAHGEPLPNPLGGDTDCNGVRDIVDVVLLIQFVVFGSPSPCCLSP"
     gene            complement(4290..5951)
                     /locus_tag="AC16FU_06100"
     CDS             complement(4290..5951)
                     /locus_tag="AC16FU_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993977.1"
                     /translation="MRVVIVLVGLFFLWISSVTADITGTITDHSSPTHSILGVAAFEQ
                     WRVSDPLFPLLLTVDAAVLADSLPPHGFTIPADPAKTYVLAAIGFVESGDDISPEAIA
                     DIRYNVAGNASGVALAITPNPNHITGRLYSGGGGSLGRDPMIWVQDTSGKFVTVGSPD
                     TAGNFSLNYLPGAGPYTLLSVAERHYVRVDANKVNPSTVGLLGLSPAGQTLITGSANT
                     GVPGQVRAVLSDRKNFELQFTVFNAPFGQALTAVTIEAPGGDPGDPPVRYYLSPVASN
                     HRLPLDLFWTSIVVETNRQPNRITFARGVGTSKIPPGASATFTLRIDSTTNVDRDTYQ
                     DWNVVVRGDTADLGRAQEPDTCSGWEEDCGLETVQRILEPYDLRFDPPSNNDGTEPVD
                     VDRTLRLWVRNCGTGVFHPYPLWTSVDPGGAFSYQQWPPTVVPSLAPGDSVAVLSQSV
                     HTTMYPGTFTLHAFVIDSTGPGVNTSYADYRESLRTCFMRGDVTVDGVIDLSDLVALV
                     QDIVFGVPLPNPTAGDANCDGVRDIVDLALLVQFVVFFSQTPCCL"
BASE COUNT         1347 a         1997 c         1894 g         1212 t
ORIGIN      
        1 gctctgccgc cccaccacgc cgaatcgaaa ttcgactgtt tcgttttcaa cggccccctc
       61 ccgcgttctg ctgcttcacc tgctcttcca gcgtctgcag gcgcgccttc atctctgcca
      121 cctctgcctt cagacgggcg ttgtgctctt caacagtgat tgtcctcact tcggactgtt
      181 ggctgccaac acctggtact cccgccattg cgcttggctc gaggccggac ggcactgagg
      241 ccacagtgct aaccaccgtg ccgtaggccg tcgggaagta ctgggccaag aggtggacac
      301 ggttaatttc ggacagagac ccgatcgcgt tgggagtcca gcggtcggcg tttaagtagt
      361 actttacggt ggttccaccg gtgacggcct cgactttggt cagactgaat gagcggtagc
      421 ccttgtcccc ggagaacgat gggtgactgc tctctacggt aaaaagtgcg aagttgtcaa
      481 agtcaatgcc gccccttacg gtggaaatga aggcccgtcc ccaagtatct gtccccgtta
      541 catgggtaag cttaaagaag ccgtgtgccg ttacgaccac gtacccattg gcaggatagg
      601 tgatggtaac cgattcaatc gggatcgtcc ccggagcgac gggcaatatg gtgctaccga
      661 acgtgaaact gtgcgctatc cccggctcgt cctttatctc cagcgagttg atggcgtcgg
      721 tggggagaat ggccgaggca tcaccggtca gcccgccgtg aagatcaatg gtcgtgagcc
      781 cgccgttatc tcgcagctcc aattcaccac ctgacgaagc gttggcggta agtcttacgg
      841 tttcgtcgtt ggttggatca gcgtcgtgca agagaatttc cccgtaacta gccccccaca
      901 gccggatctg ctcctgcccg tcactgccgt atgtggaaag ctggtggttc ccaaaagtcg
      961 caatacccac gactcccggg acgaaggcgg acggaacctc aagactcccc gccacccgcg
     1021 tgtagggagt gctgatcagc cgggtacgcg gagataatat caccgcgtcg gcttctactt
     1081 ccagccaggc ctcattgttg tcctggaaaa ggtccaccgg caagaggtca gccgggtccg
     1141 aacctaattc atacgtgaag agaccaccgg aagtcgtcac ccctccagcc actggatcgt
     1201 cccagagtag gacgccgccc accgctgtgc tgtagatgcg gaaggtgacg gcgtgcacgc
     1261 cgtcggccac cggcacgccg gaggcgtcgg tcagccgccc ctggaagtta atcaggttgg
     1321 gcactgaagg cactaaagcg aaaacacttg cggccagaag gccgatgacc acagtgaaga
     1381 gaaaaatccg tactcgcatt gcaaacctcc gtttgcggtg cacccgccag ccggtgcgcc
     1441 aaattgttgc cgcgattgct ggcaatcgcc tgaacgatca gccggaacca ttctggtcag
     1501 ttcgccatac tttacctccc cgttgagcca tccgctgctc tcttattgat tctcccccac
     1561 ttcttcaagc aggtggagaa tctttgaatc aagagataat aagtcttcga ttgccgtgca
     1621 acaaaactct cgccgaacat ggcgattcac cttacggcga caaacagcag ggcgagggtg
     1681 aaccgaacac cacgaactgg atcagcagca ccacgtccac gatgtcgcgg acgccgttac
     1741 agtcggtatc gccacctaaa gggttcggca acggctcccc gtgcgccacg tcgttgatca
     1801 aaccgatcac atcgatgatg tccaccagcc cgtcgccgtc cacatcgccg gtctggagat
     1861 tggcgccgga cgccaccgag aacgacctat tcccgttgga atcctgggcg accaccaagt
     1921 agaagtagaa ctggttgggc agagccgtcc ggtcgacata gaccgtatcg cggccgacat
     1981 tggcgaggaa aatcttggtg gctaagttga agatggtgtc gcggaagacc tcatagtgct
     2041 cgaggtccgg cgccgccacc ggatgccagg agacgaccaa agctgaacag gggagcgtcg
     2101 acgcaaccgt caccgccgga gccggcggga acagttcgtc ggtcgccacg ccggagtcgg
     2161 gatttgaatc gaagaaaacg gtactgtcgt tggctacgtt tcgaacgaaa aagacctccc
     2221 ggttggtcgg cgtctgtacc gtgggggtgt agagactttc cgtcacgacg accggcactt
     2281 ctttgatctt gctccacgtc cccgggggag tggcaaccgc gccgaagggc ggggaatcgc
     2341 gccggaagat ttggaatttc tggcccagcc cgctccccgt gggacggctc ttccagttca
     2401 attggaactg gcggccctgg tcatcaggca cgttggcgac cgaggtcaaa gtcatctcgt
     2461 agccggagag aaaggccaaa acagtgccca ggttgaaatc ggccggatcg gtaaagggcg
     2521 cgccgatggc gaagtccgca tagccgtcgg cgttcaggtc gcccagagcc gccaccgcaa
     2581 agcccagctg atcaccagcg ctggcgccgg cccgccaaag gagggtatca ccggtggccc
     2641 cggaaaaaag aaccgccgtg ccggcaaatc cgcgtcctcc gggatcggcc aaaggcgcgc
     2701 cggccaaaaa ctcggcgaat ccgtcgtgcg tggcgtcgcc cacaatcgcc acggacaagc
     2761 cgaattgatt cgaaacatca atgccgtttt tctggtagag caccccgccg gtcgcgccgg
     2821 aaaaaacgta ggcgctgccg gcgtcgttca gcccgcccgg attggcaccc ggcgcaccgg
     2881 cagccagatc cggcacgccg tcattattca cgtcgccgcc cccggccgtg ctgtacccca
     2941 gttggtcccc ggcagcggtt ccatcttttt ggaacaagag cccgccgccc gatccggaat
     3001 agacaaaaac gctgccggcg ttggagcgga acccggggct ggcccgattg gcccccgccg
     3061 caaaatcatc gaagccgtcg ccgttgatgt cccccagacc cgccaccgaa tatcccagct
     3121 gcccggccaa ttcggtgccg ttcttctggt ggagcaccga cccgtccgct cccgagaaga
     3181 cgaacacgat tccggcggag gtgatgaggt tcggactggc ggcgtgcacg ccgacgatga
     3241 aatcggcaaa tgcatcggca ttgacgtgtc cggcactggc caccgcatgg cccaattgct
     3301 ggccggaggc gttaccattc ttctggtaca ggagcgttcc gttcaagccc gaatagacgt
     3361 aggcgctccc ggcatcggcc agtgcgcccg gatcggccaa aggcgcgccg acgatgaaat
     3421 cgtcatagcc gtcgttattg acatcaccgg cgccggagac ggctaatcca aactcgtccc
     3481 ccgcggctcc cccgtctttc tggaagagga gcgccccgtc ccggccggag taaacgaaag
     3541 cgctccccgc gtcagttaga ccattgggat cggtaaaggg cgcgccgacg atgaaatcgc
     3601 cgtaaccgtc gttattcacg tcccccgccc cggcgaccgc tgtccccaga ttgtcgtttc
     3661 gggccaaacc ctccttcttg tacttcagca caaacggcca gtccaggatg aaggccgaga
     3721 cgacaactga tccctgactg ctctccctgg cagccgcttg tgggatgggg atgaaaaaga
     3781 ggctaaaagt gagtgtgagg aaaccaaaaa ccagtgaccg acgacccagg cgaaccaagc
     3841 tccgcattgc aacctcccgt tgttacgcac gtgttgaacg cagggatcac taatccctga
     3901 tccctgatct ctaatccctg ctctccatgc gggctccccc gtgcgggccg ggttatgttt
     3961 cgagcatacc ccggtctctc acctgagccg gagttgaaca tagcgagccg gatggaaaaa
     4021 gcaaagaaaa agcagccgaa cactgtggct gagttcaatt gcctttctaa tccctgattc
     4081 cccgcctctg gccatacggc ccgtctggga gtctgaacgt attgactggg catactgccc
     4141 gtgggggcgg ggtcgtgccg acctgatccg cctgagacgg aatggagctg cttttcccga
     4201 aaccggtttg gtagcggcag gttccctcgc cggagagggg taaccccgtt ttagggccgg
     4261 gagacctcgc ccctacgcac aacctgtgtc tacaaacaac acggcgtctg gctgaagaac
     4321 accacgaact gcaccaaaag cgccagatca acgatatcgc gtaccccgtc acagttcgca
     4381 tccccggcgg tgggattggg caggggaact ccgaacacga tatcctgcac cagcgccacc
     4441 aggtcgctca ggtcgatcac gccgtctaca gtcacgtccc cgcgcataaa gcaggttctg
     4501 agcgactcgc ggtaatcggc gtaactggtg ttgacccccg ggccggtcga atcaatcacg
     4561 aaggcgtgca gcgtgaacgt tcccggatac atcgtggtgt gcacgctttg ggagagcacc
     4621 gccaccgagt cacctggagc aagggaaggt accaccgttg gcggccactg ctggtaggaa
     4681 aaagcgccgc ccgggtcaac cgaggtccag aggggatagg ggtggaagac cccggtgccg
     4741 caattgcgca cccagagccg gagcgtccgg tcgacgtcga ccggctcggt gccgtcgttg
     4801 ttgctgggcg gatcgaagcg gagatcgtat ggctctaaaa tcctctgcac cgtttccaag
     4861 ccgcagtctt cctcccagcc ggagcaggta tcgggctcct gtgcccggcc caaatcagcc
     4921 gtatcgcccc gcaccaccac gttccagtcc tggtaggtgt cgcggtcgac gttggtcgtc
     4981 gaatcaatgc gcagcgtaaa ggttgccgaa gccccgggcg gaatcttgct cgtcccgacc
     5041 ccccgcgcga aagtgatccg gttcggctgc cggttggtct cgaccacaat tgaggtccag
     5101 aacaaatcca gcggcagacg gtggttggag gcgaccggcg acaggtagta gcggaccggc
     5161 ggatctcccg gatcacctcc cggcgcttcg atcgtcactg ccgtcagcgc ctggccgaaa
     5221 ggcgcattga agacggtaaa ctgcagttca aaattcttgc ggtcggagag gaccgcacgc
     5281 acctggcccg gcacgccggt attcgcgctg ccggtaatga gcgtctgccc cgccggcgac
     5341 agtcccagaa gtcccacggt ggaaggattg actttgttcg catcaacccg cacgtagtgg
     5401 cgctcggcaa ccgaaaggag cgtatacggc ccggccccgg gcaggtagtt cagactgaaa
     5461 ttccctgccg tatcgggaga accgacggtg acgaatttcc cgctggtgtc ctgcacccag
     5521 atcatcggat cccgccccaa ggaaccgccc ccgccgctgt agagcctgcc ggtaatgtgg
     5581 ttcgggttgg gagtaatcgc gagcgccacc ccgctggcat tcccggccac gttgtagcgg
     5641 atatcggcaa ttgcctccgg gctgatgtca tcccccgatt ccacaaagcc aatggcagct
     5701 aagacgtagg ttttagccgg atcagccgga atcgtaaagc cgtgcggcgg cagtgaatcg
     5761 gccaagaccg cagcatcgac ggtgagcaaa aggggaaaaa gcgggtcact gacccgccac
     5821 tgctcaaatg ccgccacgcc cagaattgag tgcgtcggcg acgaatgatc ggtgatggtg
     5881 ccggtgatgt cggctgtgac cgaagaaatc caaaggaaga acaatcccac cagcacaata
     5941 acgacgcgca tagaacactc ctttcttttg ccttgcgaac cggcaaggcc gggggtacga
     6001 acgcaccccg cgtccgtccc ccgcgaaagg aaatatatag cggattatta tatcagaaaa
     6061 gtaaaaatgg ggaattaccg gactgctttc gtacgcttct cactcacggc ggcacgggtt
     6121 cgaagatcca ccgtcacgaa gggggggctg gcggttaact cctttgcctc tctcctgacc
     6181 cctgatcgct gatcccccgc ctctggccgg ggtcgtaccg acctcatccc tgttttgata
     6241 atgactcttc ctcccctttg agggtcgaag actcgtaccg aggagggctg gggaggggtc
     6301 agacaagatg tctgacctat ggaatggaat tgttttctaa tcccccgcct ttggcggggt
     6361 cgtaccgacc tgatctctaa tacctgcttt tgtgtgggag gggttaatcg gttcttttcc
     6421 ctctcctgta ggagagggta gggtgaggtc
//
LOCUS       DBNGZA010000285         3581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1431324, whole genome shotgun sequence.
ACCESSION   DBNGZA010000285 DBNGZA010000000
VERSION     DBNGZA010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1431324"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="AC16FU_06105"
     CDS             complement(<1..1074)
                     /locus_tag="AC16FU_06105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR2993978.1"
                     /translation="MAKWMSTVAPRQWWLTASGLFLLRVGIAVWAPLGDDEGYYWVWS
                     KHLALSYFDHPPLVAWLVATSTSVLGVSLFSLRLPFVILGTLTALTLRGLIKKCIGDS
                     RLADTSSLLFQVVPVFFGIGFMVLPDASLLLFWLLTGLLFWQAQQGDKRWAWLGTGAA
                     LGGALLSKYVGVLFVISLAGYLITTQSPRAFKKMSVICALGILLFVPVLWWNAHHHWA
                     SFRYQFVARHHGSGLDGRRFSLFLGSQAIYLSPVLFLLSVCSALRLGPWRLRPEARGE
                     HFFWWLAFPTLGLFALAAAISDFKPNWPAPAYLTLLPLSLLSLARWRQRAPKAAGRIF
                     TTGLVLTLAFTALPTLHLFHPYLP"
     gene            complement(1096..1314)
                     /locus_tag="AC16FU_06110"
     CDS             complement(1096..1314)
                     /locus_tag="AC16FU_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993979.1"
                     /translation="MLSKVFLAIGVWGVAAMVQAGDKPAYTLPGAIDLAQRQNPEVLQ
                     ARQQIELARGRKLQAYALTPWRFFWPGR"
     gene            complement(1356..1796)
                     /locus_tag="AC16FU_06115"
     CDS             complement(1356..1796)
                     /locus_tag="AC16FU_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR2993980.1"
                     /translation="MRDLVKNRLQLLSQRVKKRRLRLTRQRTIVLEELAKVDTHPRAD
                     QLYRMVRQRLPTISFGTVYRNLKTLCQLELVTELKLGQDSSRFDARTDPHHHVACTHC
                     GCVVDVELPVESGLQKKAARQTGFAVSHYQTAFFGTCPRCRGKS"
     gene            complement(1847..3325)
                     /locus_tag="AC16FU_06120"
     CDS             complement(1847..3325)
                     /locus_tag="AC16FU_06120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR2993981.1"
                     /translation="MTITPPDLVFSGLVPEIVVVGFATLLMVVGPFFKQRGQDVVFWL
                     TLIGLALAAFTSILLWNQTISAMGGMAYSDNFATFFRLVFLLAAFVVVLFSKAYLKWE
                     NLPAAEYYSLLLFSTFGMMLMAGSLNLIVIFLGLEVMSIPLYILAGYRRSDFRSIESS
                     MKYFLIGAFASGFLLYGIALIYGAVGSTDLEKIAQAISSGQVATDFLFLSGVGLLLVG
                     FGFKVASVPFHMWTPDVYEGAPTAVTGFMAAGPKAAGFAALLRVFSIFLAGLNHNLMP
                     AFWILSVLTMTTGNVIALSQPNLKRMLAYSSIAHAGYILVALVAGGEAGIASALFYLL
                     AYSLMNLGAFACVCAVEKVTGRGNEIASYAGLASRHPLVAIALTIFLVSLGGLPPTAG
                     FVGKFYVFSAAVNAGQIPLVIIAVLNSLISVYYYLRPVVVMFMQAPEAVPASAPEGSA
                     TAFAWPVSLALSIFITAWGTLQLGIFPQALLELARQTVSAFL"
     gene            complement(3322..>3581)
                     /locus_tag="AC16FU_06125"
     CDS             complement(3322..>3581)
                     /locus_tag="AC16FU_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993982.1"
                     /translation="PALNRRERWTLIPWVVLIFWLGLSPKPFLSRMEGSLANLVTQVE
                     ERRGYLSQYRNGEIVSPVPQGLLTSTPQHPDTSTLNQASRP"
BASE COUNT          973 a         1009 c         1023 g          576 t
ORIGIN      
        1 gggcaaataa gggtggaaaa gatggagggt gggcagggcc gtgaaggcca gagtaagtac
       61 cagccccgtc gtgaatattc gccctgccgc cttaggggcc cgctggcgcc aacgtgccag
      121 gctcaaaagc gaaaggggaa gcagtgttaa gtaggctggt gccggccagt taggtttaaa
      181 atcagaaatg gcagccgcca gcgcaaagag gccaagagtg ggaaaagcca gccaccagaa
      241 aaagtgctca ccccgtgcct ccgggcgtaa ccgccaaggc cctaaccgca gggcggagca
      301 caccgaaagc agaaatagaa ccggtgagag ataaattgcc tgtgacccca aaaaaagaga
      361 aaatctccgg ccgtccaaac ctgacccgtg atgacgggca acaaactggt aacgaaacga
      421 tgcccaatgg tgatgggcgt tccaccaaag aacaggcaca aaaagcaaaa tccctaaagc
      481 acaaatcacc gacatttttt tgaacgcccg cggactctgg gtagttatca gatatccagc
      541 caatgaaata acgaaaagta ctcccacata cttgctcaaa agcgcaccgc cgagtgctgc
      601 tcccgtccca agccaggccc acctcttatc cccttgctgc gcctgccaga acaaaagccc
      661 ggtgagaagc caaaacaaaa gcagggaagc atccggcaaa accataaacc ctatgccaaa
      721 gaaaaccggc accacctgaa agagcaatga gctagtatcc gctaatcggg agtcaccgat
      781 gcacttcttg attagcccac gcagggtcaa tgcggtcaga gtgcctaaaa tgacaaatgg
      841 caacctcaac gagaagagag aaacaccaag aacggaagtg gaggtggcca ccaaccaggc
      901 tactaaggga gggtggtcga aatagcttaa ggccagatgt tttgaccaga cccagtaata
      961 cccctcatca tccccaagag gcgcccaaac cgcaatcccc acgcggagaa gaaacagccc
     1021 agacgcggtc aaccaccatt gccggggcgc caccgttgac atccattttg ccattgcccg
     1081 acttcaacaa cccggtcacc ggcctggcca aaagaatcgc cagggggtga gcgcataggc
     1141 ctgaagcttt cggccccgcg ccagttcgat ttgctgccga gcttgcagca cttcgggatt
     1201 ttgcctttgg gccaggtcga tggcgccggg taaagtgtaa gcgggcttgt cacccgcctg
     1261 caccattgcg gcgacgcccc ataccccaat agccaagaaa actttagaca gcactcttcc
     1321 agaaatacgg gataaagtga gaaaaaatca cacaatcagg acttgcccct gcagcggggg
     1381 caggtgccga agaaggcggt ctggtagtgc gaaaccgcaa agccggtctg ccgggccgcc
     1441 tttttctgca agccggactc caccggcagt tcgacatcca ccacgcaacc acaatgcgtg
     1501 caggcgacgt ggtggtgggg atcggtgcgg gcgtcgaacc gggaagaatc ctggcccaat
     1561 ttcaactcgg tcaccagctc cagttggcag agtgttttca ggttgcggta gacggtcccg
     1621 aacgaaatcg tcggcagccg ctgcctgacc attcggtaca gttggtcggc gcgcgggtgg
     1681 gtgtcgactt tcgccaattc ttcgagcacg atcgtccgct gacgggtcaa gcgcagtctg
     1741 cgctttttga ctcgctggga gagcaactgc aagcgatttt ttaccagatc gcgcatcaaa
     1801 gtctaatgag aagatatgtc gttaacagcc cttgtgcaaa ggcggattaa aggaatgccg
     1861 acaccgtctg gcgcgccaac tctaaaagcg cctgcgggaa aatccccagc tgcagcgtcc
     1921 cccaggcagt gatgaaaatc gatagggcta aagataccgg ccaggcgaaa gctgtcgcgg
     1981 aaccctctgg cgcggaagca ggaactgcct ccggcgcctg catgaacatc accaccaccg
     2041 gccgcaggta gtagtagacc gaaatcaaac tgttgagcac ggcgatgatg actaagggaa
     2101 tctggcccgc attcaccgcc gccgagaaga cgtaaaattt ccccacaaaa ccggcggtgg
     2161 gcggcagtcc gcctaaggag accaggaaaa tggtcagcgc tatcgcaacg agcgggtgcc
     2221 gggacgccag cccggcgtag gaggcaattt cattccccct gccggtgact ttttccaccg
     2281 cacagacgca ggcgaaagcg ccgaggttca tgaggctgta ggccagaagg tagaagagcg
     2341 cgctggcaat gcccgcttct cccccggcca ccagggccac taaaatgtac cccgcatggg
     2401 ctatgctgga gtaggccagc atccgcttga ggttgggctg ggacagggcg atgacgttgc
     2461 cggtggtcat ggtcaagacc gaaagaatcc agaacgccgg catgaggtta tgattgagcc
     2521 cggccaaaaa aatcgaaaaa acccgcagca gagcggcaaa gccggctgcc ttgggcccgg
     2581 cggccataaa gccggtgacc gcggtcggcg cgccttcata gacatccggc gtccacatat
     2641 ggaagggcac cgaggccact ttgaagccga agccgacgag aagcaacccg acgccggaga
     2701 gaaagagaaa gtcggtcgcc acctgaccgg aagaaatcgc ctgcgcaatt ttctccaaat
     2761 cggtggagcc gaccgcaccg tagataagcg caatgccgta gagcaaaaac ccgctggcga
     2821 aggcgccgat cagaaagtac ttcatcgacg attcaatcga acgaaaatcg ctgcgccggt
     2881 agccggccaa aatgtaaagc gggatcgaca tcacttctaa ccccaggaaa atcacgatca
     2941 ggttgagact gccggccata agcatcatgc cgaaggtaga aaagagcaaa agcgaatagt
     3001 attccgccgc cggcagattc tcccatttga ggtaagcttt tgagaaaaga acgacgacga
     3061 acgccgccag aagaaacacc agccggaaga aggtggcgaa gttgtcggag taggccatcc
     3121 cacccatggc ggaaatggtt tgattccata gcaaaatgct ggtgaacgcg gccaaagcca
     3181 gaccgataag cgtaagccag aagactacgt cctggccgcg ctgcttgaaa aacggcccca
     3241 cgaccatcaa gagggttgcg aaacccacga cgacgatttc cggcaccaag ccgctgaaaa
     3301 cgagatcggg aggagtaata gtcatggccg gctcgcttga ttaagtgtcg aggtgtcggg
     3361 gtgttgaggt gtcgaagtta aaagaccttg gggcactggt gatacgattt caccattacg
     3421 gtattgactg agataccccc gcctctcctc cacctgcgtg acgaggttgg caagcgaacc
     3481 ctccatccgg gagagaaacg gcttggggga gaggccgagc cagaaaatga gaacgaccca
     3541 agggattaac gtccaccgct cacgccggtt gagggccggt a
//
LOCUS       DBNGZA010000286         6658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1325098, whole genome shotgun sequence.
ACCESSION   DBNGZA010000286 DBNGZA010000000
VERSION     DBNGZA010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1325098"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1348)
                     /locus_tag="AC16FU_06130"
     CDS             complement(47..1348)
                     /locus_tag="AC16FU_06130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2993983.1"
                     /translation="MKASPTLNERAKVTDRVLSDRALAVPGSAIHLITQMIEELERKG
                     AKDIIRLHLGDPDFATPPKVIEATNQALASGKTHYTLARGLRPLREAVGKRLQEKYGL
                     AGDADLITMAAGSAQGLGVAFHAVAGYGDQILFPEIFWPNFFQQAALYGIKVGFYPLT
                     TDYEPDLDRLEACLTPETKAILINSPSNPTGAVFPEKTLRKMYDFACRHNLWVVSDDA
                     YDDIKFTGESFVIAQLERDLPEGQRRVFSIFSFSKSFAMTGFRMGYVHAPSLQAARIL
                     GNLQVPMTGALNTPAQYAALAALSEPALTKEMCAAYQRRRDLALNVLKKHGMYDYTPQ
                     GAFYLLVDISRTGLDGDAFAQKLLQEKKVAVAQGSGFGFMPQYDARGRIVRSVDEHGL
                     ARYPVNSKVAGKVRVSFCVAEEQVEEGMEKLCGFCQRISNG"
     gene            1496..3502
                     /locus_tag="AC16FU_06135"
     CDS             1496..3502
                     /locus_tag="AC16FU_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2993984.1"
                     /translation="MPKPKRLITAEDLLKFKWIRSVALSPEEKQIAYTLEWIDETKKK
                     YWSNLWVVPATGGAPRPFTQGKVKDREPVWSPDGKLIAFFSVREEKDGIYVIPADGGE
                     ARQIVTLDGSFASLSWSPDGKKILCAFRKNDVPQDGEKKDEKEKKKEAPVFRHITRLF
                     YRLDGEGFLPKDRYHIWTFDAASGKGKQLTFGRFDEFGPAFSPDGRWIAFASNHQPNP
                     DRAFLRVDLWLVPASGGKLKKIPTPAGPVESPAWSPDGKKIAYLGHTNPEDAWGVTNY
                     HPWVVPAEGIGKAKDLTPDFDRTCIDETICDMQEGFALPAPFWSQDSKRLYFVASDFG
                     STRLFSVAAKGGKVTRILNRPGHLQKYSAAPKAGIAALALADFSSPAEIWTIRLKGRP
                     NRPRQVSKISREFLSDIRLSRPEAAGFRSFDGTPVQAWIMKPPRFKPGRKYPAIVEIH
                     GGPRAQYGNTFFHEFQLLAAKGYVVFFSNPRGSQGYGEGFAGAIINDWGNLDYKDVMA
                     GVDTLVKKPFVDPKRIGVTGGSYGGYLTNWIVGHTNRFKAAVTQRSISNFYSFVGSSD
                     IGFEDHRELGAQPWTNPEELMRMSPITYVKNIRTPLLILHNENDLRCAVEQAEQFFTA
                     LKFLKRKVEFVRFPEEPHGLSRHGRPDRRLARLSWILRWFGRYL"
     gene            4022..5377
                     /locus_tag="AC16FU_06140"
     CDS             4022..5377
                     /locus_tag="AC16FU_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR2993985.1"
                     /translation="MSSTQTQCQYKVLGDSDYKCPYPVERDAYCIFHMHTRYGDDFKK
                     FNDGISTLLENQTGGAYDFRGFRFPSINFSGKVFEKPLLLDQAVFNCRLEFHKSRFSQ
                     KVDLRHAAINDWFVLSDVEFNDGVDMLGTVCREPARFFHVKFHNASFFKTTFQSVLFE
                     DSHFTGMTLFNKTVFLEPADFISSTFQLVYFVETEFDQRVLFQEGDFFKHAEFTGAIF
                     NCEAIFKGTIGKKVFRDDAIFGSLKIGKEAHITFEKINLSKASFLDTNLERITFRDVE
                     WTSRKKFWGCSGKALWDESRPLEQDEERDYEKIAENYRQLVLNHERKRDYDAAEDFHI
                     GEMAMRRKKKGASIKWLWLRPVREQMNAYNLYRLLSYYGTSYWQAVFGLLAFLFLLPF
                     GYCSTDYWQGFLRPLSILTFQRPAEQLDRWSRFWLSISVIVLTAQVALVLLAIRRRFK
                     R"
     gene            complement(5692..6198)
                     /locus_tag="AC16FU_06145"
     CDS             complement(5692..6198)
                     /locus_tag="AC16FU_06145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR2993986.1"
                     /translation="MPQLESEELVADIQVTKPQGRPLIRLFASGFGMGYLFPFPGTWG
                     SLPPVFLAWYLLSRFPTWYLVLLPLFLIAGAYVATRAERLWVHDDRRIVVDEFVGMLV
                     SLFLVPRSFGYYLAGFLLFRFLDIFKPFPARRAENLPEGWGVMADDVIVGIYANLLLQ
                     LYSLLAGR"
     gene            complement(6272..>6658)
                     /locus_tag="AC16FU_06150"
     CDS             complement(6272..>6658)
                     /locus_tag="AC16FU_06150"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2993987.1"
                     /translation="ADKILTSTAFICFVALGYASTWMVLAIVVREFLITGLRSLAAYK
                     GVVIIPSLFAQVKTVLQMSVIVVILVYINVKTILVSNGHPWAIFSSPWTPRAFNGLIF
                     LTTVVTVGTGIDYLVKSAPLLRGVLK"
BASE COUNT         1616 a         1751 c         1873 g         1418 t
ORIGIN      
        1 atttagacat tcgatttggc agatggtctg tctatataat ccgtattcat ccgttcgaaa
       61 tccgttgaca gaaaccacat agcttttcca tcccctcttc cacctgctcc tccgccacac
      121 agaaggagac ccgcaccttg ccggccactt tggagttcac cggatagcgg gccaggccgt
      181 gttcatcaac cgaacggaca atgcggccgc gggcgtcgta ctggggcata aagccgaatc
      241 cggaaccctg cgccaccgcc actttctttt cctgcagaag tttttgggca aaggcatcgc
      301 cgtccaggcc ggtgcgggaa atatccacga gcaagtagaa cgccccttga ggggtgtaat
      361 cgtacatccc gtgctttttg agtacgttca acgcgagatc gcggcggcgc tggtaggcag
      421 cgcacatttc cttcgtcagc gccggttcag agagggccgc cagggcagcg tactgggccg
      481 gcgtattgag ggcgccggtc attggaactt gcaggttgcc caggatccga gccgcttgaa
      541 gggagggagc gtgaacatac cccatccgga agccggtcat ggcaaagctc ttggagaaag
      601 agaagatgga aaaaacccga cgctggcctt cgggcaagtc tctttctaat tgggcaatga
      661 cgaatgattc gccggtgaac ttgatgtcat cgtaggcatc atcgctcacc acccagaggt
      721 tgtggcgaca ggcgaagtca tacattttcc gcagcgtttt ttccggaaaa actgcgccgg
      781 tgggattgga gggggaattg atgagaattg ccttggtctc cggggtcaaa caggcttcaa
      841 ggcgatcaag atcgggctcg tagtcagtgg tcaggggata gaatccgact ttaataccgt
      901 acagagccgc ctgctggaag aagttgggcc agaaaatttc gggaaagaga atttgatcgc
      961 catacccggc aacggcatgg aaagccacac ccagcccctg cgccgagccg gcggccatgg
     1021 tgatgaggtc ggcgtcgcca gccaggccgt atttttcctg tagtcttttt ccaactgctt
     1081 cccgcagcgg gcgaagcccg cgggccaggg tgtagtgcgt tttgccggag gcgagggcct
     1141 ggttggtggc ttcaatgacc tttggcggag tagcgaagtc gggatcgccc aagtgcagcc
     1201 ggattatgtc tttcgcgcct tttcgctcca actcctcaat catctgggtg atgaggtgga
     1261 tggccgagcc ggggaccgcc agggcgcggt cggacagaac ccgatcggtg actttggctc
     1321 gctcgttcaa agtgggtgac gctttcatcg gtggatttct ttccctaact ctttactgaa
     1381 agttctgcgc aaattatcgc cccgagggcc gaaacgcaag ttcggcttgt gtcaaacgcc
     1441 attttgatgc aaattgagcc cctcgtccgt gaacgaaacc tgaattgagg agtgtatgcc
     1501 aaagccgaaa cgtctgatca ccgccgaaga tttgctcaaa ttcaagtgga tccgctcggt
     1561 ggcgctttcg cctgaggaga agcagatcgc ctacacctta gagtggatcg acgagacgaa
     1621 aaagaaatac tggtcgaatc tctgggtggt ccccgcaacc ggcggcgcgc cgcggccctt
     1681 tacgcaggga aaagtcaaag accgggagcc ggtctggtcg ccggacggga agctcatcgc
     1741 cttcttctcc gtgcgcgagg aaaaagacgg catttacgtc atccccgccg acggcgggga
     1801 ggcgcgtcaa atagtcacgc tggacgggtc gttcgcttcg ctctcctggt cgccggacgg
     1861 aaaaaaaatc ctttgtgcct tccgcaaaaa tgatgtgcct caagatggcg aaaaaaaaga
     1921 tgaaaaagaa aagaagaagg aagcgccggt tttcaggcac atcacccggc ttttttaccg
     1981 gctcgacggc gaaggttttt tgcccaagga ccgctatcac atctggacat ttgacgccgc
     2041 ttccgggaag ggaaaacagt tgaccttcgg ccgttttgac gagttcggac cggccttctc
     2101 gcccgacggc cgctggatcg cttttgcctc caatcaccaa cccaatccgg accgcgcctt
     2161 cctgcgggtt gacctgtggc tcgttcccgc aagtggagga aaactgaaga aaattcccac
     2221 gcccgccggg ccggtcgaga gccccgcctg gtcaccggac gggaagaaga tcgcttacct
     2281 gggccacacc aatccggagg atgcctgggg ggtgaccaac taccacccct gggtggttcc
     2341 cgcggaaggc attggcaaag cgaaagattt gacacccgac tttgaccgaa cctgcatcga
     2401 tgaaaccatc tgcgacatgc aagaggggtt cgccctgccg gcgcccttct ggtcccagga
     2461 tagcaagcgc ctctatttcg ttgcgtcaga tttcgggagc acgcggctat tttcggtggc
     2521 ggccaaaggc ggcaaggtca cgcgcatcct caatcgtccg ggccacctgc agaaatacag
     2581 cgccgcgccc aaagccggaa tagctgcctt ggcgctggcc gatttttctt cccctgctga
     2641 aatctggacg atcaggttga aaggccggcc caatcggccc cggcaggtct caaagatcag
     2701 ccgcgaattc ttaagcgata tccgcctttc ccggcctgag gcggccggtt tcagaagttt
     2761 cgacggcacg ccggtgcagg cctggattat gaagccgccg cgcttcaaac cggggcggaa
     2821 gtatccggcc atagtcgaaa tccacggcgg gccgcgggcg cagtacggca ataccttctt
     2881 tcacgaattt caactcctcg ccgccaaagg ttacgtcgtc ttcttttcca acccgcgcgg
     2941 gagccaggga tacggcgagg ggttcgccgg ggcgatcatc aacgactggg gcaacctcga
     3001 ctacaaagac gtgatggcgg gagtcgatac gctcgtcaaa aaaccgttcg tcgatcccaa
     3061 gcgtatcggc gtgaccggcg gaagttacgg cggctacctg accaactgga ttgtggggca
     3121 tacgaaccgt ttcaaagcgg cggtgaccca gcgctcgatt tcgaatttct acagtttcgt
     3181 cggctcctcg gacatcgggt ttgaagacca ccgcgaattg ggggcccagc cctggaccaa
     3241 tcccgaggaa ttgatgcgga tgtcgcccat cacctacgtc aagaatatcc ggacgccgct
     3301 attgatcctg cacaacgaga acgacctgcg ctgcgcggtt gagcaggcgg agcagttctt
     3361 caccgcgctc aagtttttga agcgcaaagt ggagttcgtc cgctttccgg aggaaccgca
     3421 cggcctctcc cgccacggcc ggcccgaccg gcggctggcg cggctctcgt ggatactgcg
     3481 gtggtttggg cgatacctct aaaagccata ttccaccaca gaggcacagt cggagactcg
     3541 actcttcgag aggacacgga gaagacaaga gttccgggaa atgggcgggg tttcaatctt
     3601 ttatctttct ctaacgcaga caagggctta cgacaaaaaa gctgaacacg gtattcggca
     3661 cacaggaaaa ggcaaaaaac cacctcttct ctggattcgt gagtgaggcg tgcccttcta
     3721 ttatttggca cattgtccgt tttacccccg gaacgacccc aaataaaaca gtgatttgtg
     3781 atgagtgaca agggattagc gaaaacgttg atcggagcta aagccccgcc tacgcggagg
     3841 aaggcggtag aattggaccg actcaggtcg gtacgactct atcgaggacg agccggtcta
     3901 cctagaggtg gggagagaat tcacagcaga tggagcaaag ccatgaaaat gaaaccaagt
     3961 aagaaaagta aggataacga ctgagggaag tgcaggtctt tagtgtattt tatcactcac
     4021 tatgagcagt acacagacgc agtgccaata caaagtgcta ggtgattcgg attacaaatg
     4081 tccctaccct gttgaaaggg atgcctattg tatctttcat atgcataccc gatacggtga
     4141 tgattttaag aaattcaatg atgggatttc taccttgttg gaaaatcaaa ccggaggagc
     4201 atacgatttt agaggttttc gctttccctc aatcaatttc agcggaaaag tatttgaaaa
     4261 acccctctta cttgaccaag cagttttcaa ctgtcgctta gagtttcata agtcgcgatt
     4321 ttcccaaaag gttgacttgc gtcatgcggc cataaacgac tggtttgttt tgagtgatgt
     4381 cgaattcaac gacggggtcg atatgctcgg aactgtttgt cgagaaccgg cgcgtttttt
     4441 ccacgtcaag tttcacaacg ccagcttttt caaaaccact ttccaatcag tactctttga
     4501 agattcgcac tttacaggga tgactctctt taacaaaaca gttttccttg aacctgctga
     4561 tttcataagc tctaccttcc agttggttta ttttgtggaa accgaatttg atcaaagagt
     4621 tctctttcaa gagggggatt tcttcaaaca cgctgaattc acaggagcca tatttaattg
     4681 cgaggcaatc tttaaaggga ctatcgggaa aaaagttttc agggacgatg ctattttcgg
     4741 gtctcttaaa ataggcaaag aggcacatat cacttttgaa aaaataaatc ttagtaaagc
     4801 aagctttcta gatacgaacc tggagcgaat aaccttccgt gacgtggagt ggacctcgag
     4861 aaagaaattt tgggggtgct caggaaaagc actgtgggat gagtctcgac ctttggaaca
     4921 ggacgaagag cgcgattatg aaaaaatcgc ggaaaactac cgacaattag tgctaaacca
     4981 tgaacgtaag cgagattacg acgccgcaga ggatttccac ataggcgaaa tggcgatgcg
     5041 gaggaaaaag aaaggggcga gcatcaaatg gctttggttg cgacccgttc gagaacagat
     5101 gaacgcctac aatctctacc ggctactgag ttactacggc acgagttact ggcaggcggt
     5161 ttttgggctt ctcgcatttc tattcctctt gccgtttggt tactgttcta ccgactactg
     5221 gcagggattt ttgcgcccgc tatctattct cacctttcag cgacccgccg aacaacttga
     5281 ccgatggtcg cgcttctggc tgtccatctc ggttatcgtt ttaaccgcac aggtggcgct
     5341 ggtgcttttg gcgatacggc ggcggtttaa gcggtgagct gacctcacct caccctaccc
     5401 tctcctacag gagagggaaa cgagcggaac ggtagacccc tccctcgccc tcccccgcct
     5461 ctggcgggat ctggcgaccc tcaaggggag ggaaaggttc acctcatatt ctgtaagtca
     5521 tgcgggatag gaaaaccatc ccgcactacg gggtaatttt actttcagaa cggagataag
     5581 cacgtagtgc gggttggtct tgtaacctgc gcgtacggga ttggaaaacc atttcggtac
     5641 gagtccatac ggatccgtag ggatcgtgcc gacccgcact acggggtaat atcaccgtcc
     5701 tgccaacaga ctgtacaatt gcaaaagtag attggcgtaa atccccacaa tcacatcatc
     5761 cgccatgaca ccccagccct cgggcagatt ttcggcgcgg cgggcgggga agggcttgaa
     5821 aatatccaga aagcggaaaa ggagaaaacc ggcgagatag tagccgaaac ttcgaggaac
     5881 taaaaagagg gacacgagca tcccgacgaa ttcatcgacc acaatccggc ggtcatcatg
     5941 cacccagagg cgttcagccc gggtggccac gtaggcgccg gcaattaaaa agagcggcag
     6001 aaggaccaag taccacgtcg ggaaccggga taagagatac caggcgagaa aaaccggcgg
     6061 cagggagccc caggtgcccg ggaaaggaaa gaggtagccc atcccgaagc cggaggcaaa
     6121 aagtctgatc aaaggccttc cctggggctt ggtcacctga atgtcggcca ctaattcttc
     6181 cgattcgagt tgcggcataa tttttctttc agcaagagcc accgggggcc gaggacggaa
     6241 tcctcggcta cagaaaatcg ccgggttttt atcactttag cactccgcgc agaagcgggg
     6301 cggatttcac cagatagtcg atgccggtgc cgacggtcac caccgtggtc aggaaaatca
     6361 aaccgttgaa ggcgcgcggg gtccaggggc tggagaaaat ggcccagggg tggccgttgg
     6421 agacgagaat cgtcttaacg ttaatgtaca cgaggatgac gactatgacg ctcatctgga
     6481 gcacggtttt cacctgcgcg aaaagcgagg ggatgatcac cactcctttg tatgcagcga
     6541 gcgagcggag gccagtgatc agaaattccc gcactacgat ggccaaaacc atccaggtcg
     6601 aagcgtagcc cagggcgaca aaacaaatga aggcggtgga ggtgagaatc ttgtcggc
//
LOCUS       DBNGZA010000287         3110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_900105, whole genome shotgun sequence.
ACCESSION   DBNGZA010000287 DBNGZA010000000
VERSION     DBNGZA010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_900105"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1413)
                     /locus_tag="AC16FU_06155"
     CDS             complement(91..1413)
                     /locus_tag="AC16FU_06155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR2993988.1"
                     /translation="MASKNWRKNVTEKIETKKAVVGVIGLGYVGLPLAVEFAAGGFVT
                     YGIDTSPEKIALINAGQSDIDDLKDDDIAPLVDAGRLTAISDYTLLKKVDCVAICVPT
                     PLSKTKDPDVSYIIGAAESLRPHLHPGMLVVLESTSYPGTTEELILPMLVNEKLKVGR
                     DFFLASSPERIDPGNPLFTTKNIPRIVGGVTPACTWVARTFYQAIVQNVHTVSSPRAA
                     EMVKLLENTFRSVNIGLVNEMALMCDRLKIDVWEIIEAATTKPFGFMPFYPGPGLGGH
                     CIPIDPHYLSWKLKTLNYYARFIELAGDINSHMPEYVVERIIRALNDRKRSLNGARIL
                     VLGVTYKRDIQDIRESPALDVIRLLQDRGAQVTYNDPHVPKVELDSIEMRSTKLSAGL
                     IRTVDCVTIVTDHSAYDYDWVVANARLVLDTRNATKDVTQGREKIVKL"
     gene            complement(1422..2378)
                     /locus_tag="AC16FU_06160"
     CDS             complement(1422..2378)
                     /locus_tag="AC16FU_06160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR2993989.1"
                     /translation="MRVLITGIAGFAGSHLAELLVREGWEVAGSTLDEQMPNLAAVAD
                     RVKIYPCDLRIREGVTVVLKDFQPEGICHLAAFSSVGESFQAPFDTFETNIVGTLNLL
                     ESAKEICPQTRLLVVTSSDIYGKVARKNLPLKETMPLDPTSPYAASKAAVDFIARQYH
                     ASFGLPVIRVRPFNHTGPRQRLGFVVPDFAHQIARIEAGLAPPTVRVGNLSAQRDLSD
                     VRDIARGYRLLLAKGRPGEVYHLSSGKAVGIRSVLKLLLSYARKKIAVAADPSRLRPQ
                     EIPILVGDSKKAKKEVGYRPAIPLTQTLRDTLEFWRSEINRK"
     gene            complement(2407..2976)
                     /locus_tag="AC16FU_06165"
     CDS             complement(2407..2976)
                     /locus_tag="AC16FU_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR2993990.1"
                     /translation="MRRLALVLLGAGFLLLGGITAGAQEKKIGFVDSRKIRDGFKEFQ
                     KGQDELDSVIAGYQKKEEELRKEIEDLQDELERQRLVLSETKRKEKEQELTQKQKVYQ
                     DWYTSTFVGPQSLLEQERQKRSRPILDKILTACKKVGQANGYSFVFDSAAGSLLYGDA
                     AFDLTDKVLEELNKSAAPTPTPTPPKGQR"
BASE COUNT          582 a          888 c          925 g          715 t
ORIGIN      
        1 ggaggggaat tagggaaaca ctgcgggtgc acaaagagta tcggttttct ccgtgcactc
       61 tgtgcttctg tggtgaaagt cttggctttt ctacaacttc actatctttt cccgtccctg
      121 agtaacgtct tttgtcgcgt tacgggtgtc cagcaccagg cgggcattgg ccaccaccca
      181 gtcgtagtcg taggcgctgt gatcggtgac gatcgtaacg cagtcgacgg tccggatcag
      241 gccggccgag agtttcgtcg agcgcatctc gatggagtcc agttcaactt tgggcacgtg
      301 cgggtcgttg taggtcacct gcgcgccgcg gtcttgcaag aggcgaatga cgtccaaagc
      361 cggtgattcc cgaatgtcct ggatgtcccg cttgtaggtc acgcccagca ccagaatcct
      421 ggcaccgttg agacttcttt tccggtcgtt gagcgcccgg atgattcgct ccaccacata
      481 ttccggcata tgcgagttga tgtcgccggc cagttcgatg aaacgggcgt agtagttcag
      541 ggtcttcagt ttccatgaga ggtagtgggg gtcgatagga atgcagtggc cgcccaaccc
      601 cggtccgggg tagaacggca tgaagccgaa gggcttggtg gttgccgctt cgatgatttc
      661 ccagacgtcg attttgagcc ggtcgcacat taaagccatc tcgttcacca ggccgatatt
      721 gaccgagcgg aaggtattct ccaggagttt caccatttcc gcggcccggg gagaggaaac
      781 ggtgtggacg ttttggacga tagcctggta gaacgtgcgg gccacccatg tgcaggcagg
      841 ggtgacgccg ccgacaatcc gggggatgtt cttggtcgtg aagaggggat tcccggggtc
      901 aatccgctcc ggggaggaag ccagaaagaa atcccggccc actttcaatt tttcgttgac
      961 cagcatcggc aggatcagct cttcggtggt cccgggatac gacgtactct ccaggacgac
     1021 cagcatcccc gggtgcaggt gaggcctgag actttccgcg gcaccgatga tgtaggagac
     1081 atccggatct ttggttttgg acaggggtgt gggcacgcaa atggcgacgc agtccacttt
     1141 cttcaacagc gtgtagtcgc tgatggcggt gaggcgcccc gcatctacca acggggcgat
     1201 gtcatcatct ttcaggtcat cgatgtcgga ttggccggcg ttaatcaggg cgattttctc
     1261 cggggacgtg tcgatgccgt aggtaacaaa accgccggcg gcaaactcca ccgccagggg
     1321 caggcccaca taccccaaac cgatgacccc caccaccgcc ttcttggtct caattttttc
     1381 ggttacgttt ttccgccagt ttttggatgc catagcctgc tctactttcg gttaatttcg
     1441 ctccgccaga actccagggt gtcacgtagg gtttgcgtca ggggaatcgc cggccgatag
     1501 ccgacttctt ttttcgcctt cttgctgtcg ccgactaaaa tcggaatttc ctgcggccgc
     1561 agccgggatg ggtcggcggc caccgcgatt tttttccggg catagctcaa tagcagtttc
     1621 aatactgagc ggatgccaac cgctttgccg ctggagaggt ggtagacttc acccggacgg
     1681 cctttggcga gcaaaagccg atagcccctg gcgatatcac ggacgtcgga gaggtctcgc
     1741 tgggccgaga gattgcccac ccgcaccgtc ggcggagcca ggccggcttc gattcgggca
     1801 atctgatggg caaagtcggg taccacaaag cccagccgct gccgcgggcc ggtgtggtta
     1861 aacggccgca cccggattac cggtaaaccg aaactggcat ggtactgccg cgcgatgaag
     1921 tcgaccgcag ctttgctggc ggcgtaaggg ctcgtggggt caagcggcat cgtctctttt
     1981 aacggcaggt tcttacgtgc caccttgccg tagatgtcgc tggaggtgac gaccaaaagg
     2041 cgcgtttgcg gacaaatttc cttggccgat tccagcaaat tgagggtgcc gacgatgttc
     2101 gtctcaaagg tgtcaaacgg cgcttggaac gattctccca ccgaactgaa ggcggccagg
     2161 tggcaaattc cttccggctg gaagtccttg agcacgaccg tcacgccttc tctaatccgc
     2221 aggtcgcagg ggtaaatttt tacccggtcg gcgacggcgg cgagattggg catttgttcg
     2281 tccagggtgc tgccggccac ttcccagcct tcacgcacca ggagttccgc caggtgggaa
     2341 ccggcgaagc cggcaatgcc ggtgatcaac acgcgcatgg caggtggcgg gggaaaaccg
     2401 cttcggctac ctctgtcctt tgggcggcgt gggagtgggt gtcggagcgg cggacttgtt
     2461 caactcttct aacaccttat cggtcaggtc aaaggccgca tcaccgtaca ggaggctacc
     2521 cgcggctgaa tcgaacacga aggagtagcc gttggcctgg cccacctttt tgcaggcggt
     2581 cagaatcttg tccaatatcg gacgggagcg cttctgcctt tcctgttcca aaagactctg
     2641 cggaccgaca aaggtggagg tgtaccagtc ctggtacact ttctgcttct gagtcagctc
     2701 ctgctccttt tccttgcgtt tggtttcgct caaaacgagc cgttgccgct ccagttcgtc
     2761 ctggaggtcc tcgatttcct tccggagttc ctcttctttc ttctggtagc cggcaatgac
     2821 cgagtcgagt tcgtcctggc ctttctgaaa ttccttgaac ccgtcgcgga tcttccgcga
     2881 atcgacaaag ccgattttct tttcctgcgc tccggcggtt attccaccaa gcagcaggaa
     2941 cccagctccc agaagcacca gcgccaatct acgcaccata cctcccttgc tgagcgtccc
     3001 acatattact atttgtcttg gtctcgttgt ctcgcagagg accggtcagg attctcctgg
     3061 tcactggacc ctgactcccg gcgaccgagt tttggtaccc ggcggctgcg
//
LOCUS       DBNGZA010000288        15627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1217818, whole genome shotgun sequence.
ACCESSION   DBNGZA010000288 DBNGZA010000000
VERSION     DBNGZA010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 15627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15627
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1217818"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..2734)
                     /gene="lon"
                     /locus_tag="AC16FU_06170"
     CDS             complement(308..2734)
                     /gene="lon"
                     /locus_tag="AC16FU_06170"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR2993991.1"
                     /translation="MPLVKEPVKTEEPKIKLDSLPVLPTRGIVVFPAFLTPLLITEQR
                     YAKMIDEALMDGQPIGLFAQKNAEAENPTPNDLYGVGTAGLILKMLRFPDGSVRFLVQ
                     GVSRIRIKRVERTEPFLVVRAEEIPEKLEPSVEVEALVRNVLELLKKVVSLAPYLPED
                     LQISALNTEDPAKLADLIGNNLNITLEQKQDLLETSDVKERLRKVLVYLNKEVEVLEL
                     SRKIQTEAASELGKTQKEYILREQLKAIQKELGEGDERTQELEEFRKKIETGGLPELA
                     KEAAQRELDRLSRMHPSAAEYTVSRTYVDWLVNLPWIKSTQDVLDIPGAQKILDEDHY
                     DLEKVKERMLEYLAVRKLKAETKGPILCFVGPPGVGKTSLGRSIARALGRKFYRISLG
                     GMHDEAEIRGHRRTYIGALPGRILQGIRRADSNNPVFMLDEIDKIGQDFRGDPAAALL
                     EVLDPEQNNTFSDHYLDVPFDLSKVMFITTANILDPIPPVLRDRMEVIELPGYTDLEK
                     LMIAKKYLLPRELENHGLKPEQLAITDEAIKHVINDYTRESGLRNLDRELATICRKTA
                     KFIAAGEKEKVKVTPDTLHEFLGPVKFLQELATRTAQIGVVPGLAWTPSGGDILFVEA
                     TKMRGKNNLTLTGHLGDVMRESAQAALSYVRSSAGKLGLPEDIFEKYDVHLHVPQGAI
                     PKDGPSAGITMATALASLFTERSVKPQVAMTGEITLRGLVLPIGGLKEKALAAYRAGI
                     KTVILPKQNEKDLEEIPEEIRKNVQFIFVEKVEQVFDYVLNSAPVTRKGNQIPRLPDV
                     GVNQIAAQKR"
     gene            complement(2775..3227)
                     /locus_tag="AC16FU_06175"
     CDS             complement(2775..3227)
                     /locus_tag="AC16FU_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2993992.1"
                     /translation="MPEERKDIFRDIRHIQEEMDLLFDHFYKIRTSPILTSHRLWRPH
                     IDVFNLNKEMVVICELAGVQESEVAIALAHNILTIRGERAQKKFAERAACHNCEIPTG
                     KFERNVHLPEPGDPEKVAALLHDGMLEIRVGKLPQNREKSRTVEIEPE"
     gene            complement(3301..4095)
                     /locus_tag="AC16FU_06180"
     CDS             complement(3301..4095)
                     /locus_tag="AC16FU_06180"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR2993993.1"
                     /translation="MRGKQTSHLLAIDIGNTHTVIGVFAGERLRAHFRLASNHALTAD
                     ECGILVTGLLANHAKRPIKPGGICLASVVPPLTPIYEEMAQKYFGVQPVTVSAAMKLG
                     IKVRTTDPRQVGADRLANAVAAYHLYGGPTIVVDLGTATTFDAISQQGEYLGGAIAPG
                     IETSATDLFRRAAQLFSVRLTRPARAIGKTTEESLLAGIVYGTAGQVDFLVRKLEAEL
                     GRQAAVVATGGLADLVVKDSETIQKVHPTLTLEGLRLIYARNRGRA"
     gene            complement(4196..4321)
                     /locus_tag="AC16FU_06185"
     CDS             complement(4196..4321)
                     /locus_tag="AC16FU_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2993994.1"
                     /translation="MSRIETATVTKAAVDFFFHMVFSFVKPGANRLAQLNVTYLY"
     gene            4353..5873
                     /locus_tag="AC16FU_06190"
     CDS             4353..5873
                     /locus_tag="AC16FU_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2993995.1"
                     /translation="MKEKQLYYGIKAVAAPEQFKQFEKLSGRERAEWLRMFWAPKDPT
                     PTTPENEFYEEHQRRVAHAVEIFCTARPGPPWDDRGEIYIRYGEPDERNFRVERHYSR
                     QMAAHSAYELRDVISDPLKYRSLVAHRLANPERDFGGDISDEPSASIARGSFTLRPRD
                     EDLLRDPKAEFLSTRLEFAAVSGEVWRYYRYGLTFQFQDEQNLGYFGLVPYIDAFGTA
                     QDYQQFVRQAVVSLDVQKEIYQHDYGGKAMDYAFTVIRFRAPNKVNYVVDVNLGLPLE
                     KLGVGGDSVTGEKVECMRRIVLYDENYQPLATDSTTLAAPVSADSRKGRLLLEQKRYE
                     LKPGRYILAVEVRDLATQKIGIYKKELLLPEYIAPDVQEISDVELASHIRPANEWDKK
                     YRHKELVVMPQPSRVFFPDQTLQYYFEVYNLKKDKDGKARFSVTPEVYGYRTRKVEYS
                     FNPRDLEADTTDIYQTGKLNLRDLTPGEYILSLKVKDRITGKEKTALAGFKVAKSE"
     gene            6038..6607
                     /locus_tag="AC16FU_06195"
     CDS             6038..6607
                     /locus_tag="AC16FU_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR2993996.1"
                     /translation="MSNGTGLTKKQMVMFAVLIGVAVFLGVYFGIYFGSRGLGRHGGE
                     APVEAKFKANDPLPEVAVLDVNGTLEPLSKRISGKGAVVMFMSLGCHPCQQESEKWQK
                     ILAEKHPEEFAVLGVCYDKTEQIQAYLKEHGFTFPIFCDSARLFMRDWSVAQFPTVAV
                     VNREGKIAKVYSGFHPDFDDKEVKKVLKT"
     gene            6660..8711
                     /locus_tag="AC16FU_06200"
     CDS             6660..8711
                     /locus_tag="AC16FU_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2993997.1"
                     /translation="MLRGWRIYSAAMRSALVFFFCVWIGSPALSTPSPSRRVSKSRVS
                     DESATTGPEQTRQKLVRIAVLEDQGWLGYGQLLRYLKDAEPVVRQRAARSCGIIQDSL
                     ACPVLIQALKDRDSTVRQEAAFALGQIQCREAKGALLKATRDRSPTVRQLALEALGKL
                     GDTTVVDRIAKFLSDPSSALRGEAALALARLKSHQAIAPLARAARDWDPAVRWKAVYA
                     LFRQNDSVTIPILEAKFTDNDPYVRAFAARGLGQLHASAPAMVPLLSDPDWRVQVQAV
                     RALGQSKASESLLFFLEYDRREGTGPLSNPYLVGEELTALGSVHSDSAYQVVRAFLAS
                     PIPDLRARATAALAQIDSARALEDLKPLRSDTNWHIRAQAAEAFGAIRTPEAFALLES
                     LFVDPDYRVRYPALEAMGKFPTERWEPFVKRALGEDEQALIATASEIAGKAHDSTLLN
                     DLVVGFWKHRMDRETDAKIAILEALERFVDAGRLSGTIAQALDFGLQDTNRLVRQLAV
                     KIFRKAGLERGGFTGTFVSDMDAEKYRAVYEQYTTNPKAAITTGKGIIVLELLPREAP
                     RTVANFIKLAQTGFYDNRVWHRVVPNFVIQDGCPRGDGWGGPGYTIRCEYNKLHYERG
                     TVGMALSGKDTGGSQYFITQSPQPHLDGRYTIFARVVEGMEVVDKIMVGDKIEKVTII
                     E"
     gene            8731..9213
                     /locus_tag="AC16FU_06205"
     CDS             8731..9213
                     /locus_tag="AC16FU_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR2993998.1"
                     /translation="MELKTVRLAMPKEANVIFGQTHFIKTVEDLYEILVGMLPGIKFG
                     LAFCEASGPCLVRAEGNDGELKAAAIAGAQQVASGHTFVIFLRQAFPINVLNAIKNCS
                     EVCSIFCATANPVEVVIAQTEQGRGVLGVIDGFPPKGVESDDDLTRRREFLRKMGYKK
                     "
     gene            9289..9636
                     /locus_tag="AC16FU_06210"
     CDS             9289..9636
                     /locus_tag="AC16FU_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR2993999.1"
                     /translation="MAGFRDLKVWQKAFTLSVEAYRITKNFPAIEQYALSSQLTRAAV
                     SVPANIAEGYDRTSKKEYRYYVSIAKGSLAEVETYFLLAMELGYITREDFNKIDSLRA
                     EVGRILTGLLKSI"
     gene            9763..10782
                     /locus_tag="AC16FU_06215"
     CDS             9763..10782
                     /locus_tag="AC16FU_06215"
                     /inference="COORDINATES: protein motif:HMM:NF019159.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR2994000.1"
                     /translation="MDFDSDLKIPGHDFYLDAPRAVPCSFVSHAHSDHVRRHGKMVAT
                     PETILLCRLRFKSASGGRARAVLFGQPTKIDDLKIELFPAGHMLGAAQILVQLDDQKI
                     VYTGDFNLQPGYTCPGAEVKPCDVLIMESTYGRPHYRFPEREKVAQQICAFIDAAFAS
                     RKIPVLLAYAVGKAQEVMKLLGDRGYKMAVHKSIFDVARVYQRCGVELTNYDLFSDPL
                     LPETVLIFPPFFWKLPPVAKIENRRVAFLSGWGIDPQAKQLYHADEVFPFSDHADFAD
                     LVAYVQKANPKKIYTLHGFSEFAETLRRMGFDAEYLPESPVRKVPLEAASPQEPVAES
                     DLFST"
     gene            10860..12812
                     /locus_tag="AC16FU_06220"
     CDS             10860..12812
                     /locus_tag="AC16FU_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR2994001.1"
                     /translation="MAQVTVESRPQPTLAQSYQPYIKASENVSEFTLRALILGSILAL
                     VFGVANAYLGLKVGMTVSASIPAAVMSMAILRGVLKRGTVLENNIVQTIGSSGESLAA
                     GVIFTIPAFLIWQASEPEFTFKIANWQIVVLSILGGFLGILFMIPLRRHLIVKEHGIL
                     PYPEGTACAEILVAGDEGGSKARTVFAGLGIGGLYKALMSGGKLWPDNFHFTPGFFKG
                     SQMGVDATPALMGVGFIIGPRIAALMFAGAVLGYVGIGPLLAFLGNYLTTPVPPATVP
                     MAQLSPAELRLYYIRYIGIGAVAMGGFISLLRSFPSIISSFKQGIGDFAARGATATVR
                     TDQDLSLKLVLGGSFLVAVAIWLLPGTQMHFVGALLAVLFGFFFVTVSSRMVGIVGSS
                     SLPVSGMTIGTLIVASVILLAYGVSGTAGMVGAMMVGTVVAIAVSMSGDASQDLKTGF
                     LVGATPKKQQIAEFVGVLVPAVIMGYVLLFLNKTHGFVGPGAFEAPQANAMSAVVKGV
                     MTGNLPWIFVIVGTMVGLSIELLGVHALPVAIGLYLSPDLSTPIVIGSLIYLAVQTFS
                     NGGLKNSRIEKGILFGSGLVAGDALVGVGIAFLVGSISSYEAFFRREDNIGTWLGGYG
                     QIFTLGIFLLLGLGYLYHIWRMKARA"
     gene            12849..13322
                     /locus_tag="AC16FU_06225"
     CDS             12849..13322
                     /locus_tag="AC16FU_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2994002.1"
                     /translation="MTSNELKHFLEQWEREYVTTVKVLQAIPDDRLDYKPAEKSRSLR
                     DLMWHIVGVENFAATLALTGAYPKERLPPPATVKELLEFYERQHKALVSQVAGAEAAL
                     LSRTVDFFSRPVPVSHILWRVLLYHQIHHRGQLSVYLRLVGAKVPSIYGPSADES"
     gene            complement(13360..13962)
                     /locus_tag="AC16FU_06230"
     CDS             complement(13360..13962)
                     /locus_tag="AC16FU_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994003.1"
                     /translation="MKKTRKIAQAALQVGGIILVVGWGLVGCTKKSPTGPVEAPATLA
                     PDKIPSEAASPPPYFQVQPYQDDGTGDSLTFLSAQVSRIVGKGGSKILIPLGQDTSVL
                     QIPHGALTSKVMITIKAQKTLAPDSSWQSTDYVILPAHLTFAKPATLTHQTKGLRNGT
                     TLRLLEYQNPVWNQLGTANIIKTKATFKNLSRAGTYRVQP"
     gene            14596..>15627
                     /locus_tag="AC16FU_06235"
     CDS             14596..>15627
                     /locus_tag="AC16FU_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR2994004.1"
                     /translation="MAEVPCGRFASGAVGLLWSNGQARAGKGGEVMRVNFFLKVFGTI
                     FLLLFNSALRAQETSFSTTHNYELPGPFGVAVADLTGDGQVDIAVSTTSGAIGLVNFG
                     QQQFVPALTFSGTNLGNLAVADLDGDGRLDLVAPDRNESVIVFLATGPGQFAPGVAHA
                     VGSVPTQVAASDIDGDTYPDLIATDFGDNTISILKDYGDAVYGPPHVFAAGQKPYALV
                     ATDFDGDGRPDIAVTNQGSNTVSIFTNVGGYAWFAPAVAYAVGLGPAGLAAADFNGDG
                     APDLAIANSASDDIALLLNNGDGTFAPARYFPAGDSTWALTAVDLDRDGDFDLVAGSL
                     GSHELGLFSN"
BASE COUNT         3103 a         4403 c         4677 g         3444 t
ORIGIN      
        1 atacatctgt ggctcacctc ctctgagcta gagttggacg gacccccttt cttcaatcaa
       61 gggggttata attcgctctt ttttgagcgg gcagactcac cgccccttct caccagacag
      121 aaaataataa atggcgatag aacctgtcaa gtagtttttt taagaaaagt gataaattac
      181 agtttattca caactcacta tggcacaatt ggttaacgga aattttcgtg gtggcctgtc
      241 ttagaaaaat cgtatgcggg tgatgaaaga ggtcagattg agggggccaa gaaaaattgc
      301 cggggggtta ccgtttctgc gccgcaatct gattgacccc gacatctggc aggcggggta
      361 tctggtttcc cttccgggta accggggcac tgttcagcac atagtcaaaa acttgttcga
      421 ccttctccac aaaaatgaac tggacattct tacggatttc ttccgggatc tcttctaaat
      481 ccttttcgtt ctgcttgggg aggatcacgg ttttgatgcc cgcgcggtaa gcggccaggg
      541 ccttttcctt caagccgccg atgggcagaa ccagcccgcg cagcgtgatc tcgccggtca
      601 tcgccacctg aggcttcacc gacctttcgg taaaaagcga agccagggcg gtggccatgg
      661 tgattcccgc cgacgggccg tctttgggaa ttgcgccctg cgggacgtgg aggtggacgt
      721 cgtacttctc aaagatgtct tcgggaaggc ccagtttccc ggccgaggag cggacgtagg
      781 agagcgccgc ctgggccgac tcgcgcatca cgtcgcccaa atgcccggtg agggtcaggt
      841 tgtttttgcc gcgcattttg gtggcttcca caaagagaat gtcgccgcct gagggggtcc
      901 aggccaggcc cgggacgacg ccgatctgcg cggtgcgggt cgccagctcc tgaaggaatt
      961 tcaccgggcc cagaaactcg tgcagcgtat ccggcgtcac ctttactttc tctttttccc
     1021 cggcggcgat gaacttggcg gtcttgcggc aaattgtggc cagctcccgg tccaaattcc
     1081 gcaagcccga ttcgcgcgtg tagtcgttaa tgacgtgttt gatggcctcg tcggtgatgg
     1141 cgagttgctc cggttttaag ccgtgatttt ctaattcgcg cggcaggagg tactttttgg
     1201 cgatcataag tttctccaaa tcggtgtagc cggggagttc gatcacttcc atccggtcgc
     1261 gcaaaaccgg cgggatcggg tccagaatat tggccgtggt aatgaacatc accttcgaga
     1321 ggtcaaaggg gacgtccagg tagtggtcgg agaaggtgtt gttctgctcc gggtcgagca
     1381 cctcaagcag ggccgcggcc gggtcgccgc ggaagtcctg gccgatttta tcgatttcgt
     1441 ccagcatgaa aaccgggttg ttcgaatcgg cccggcggat gccctggaga atccggccgg
     1501 ggagggcgcc gatgtaggtg cggcggtggc cgcgtatttc ggcttcgtcg tgcatgccgc
     1561 ctaaggaaat gcggtagaat ttgcgtccca gagcccgggc gattgaccgt cccaaagacg
     1621 ttttccccac gcccggcggc cccacaaagc agaggatcgg ccctttggtt tcggctttca
     1681 acttccgcac cgccaggtat tccagcatcc gttccttgac tttctccaag tcgtagtggt
     1741 cttcgtccaa aattttctgc gcgccgggga tgtccaagac atcctgagtg ctcttgatcc
     1801 aggggaggtt caccagccag tcgacgtagg tgcgggagac ggtgtactcg gcggcggagg
     1861 ggtgcatgcg gctcaggcgg tcgagttccc gctgggcagc ttccttggcc agttccggca
     1921 ggccgccggt ttcaattttc ttccggaact cttctaactc ctgcgtcctt tcgtcgcctt
     1981 cacccagctc tttctggatc gctttcaact gctcgcgcag gatgtactct ttctgggtct
     2041 tgcccagttc gctcgccgcc tcggtctgga ttttgcgcga gagttccagg acctccacct
     2101 ctttattgag gtagaccaac accttgcgca gccgttcctt gacgtcggag gtttccaaga
     2161 gatcctgctt ctgttccagg gtgatattga ggttattgcc gatcaggtcg gctaatttgg
     2221 cggggtcctc ggtattcaag gccgagatct gcaggtcctc cggcaggtag ggggccagac
     2281 tcaccacttt tttcaaaagt tccagaacgt tgcggaccag cgcttccacc tcgactgagg
     2341 gctccagttt ttcgggtatt tcctccgccc ggaccaccaa gaaaggttcg gtccgctcta
     2401 cccgcttgat tctgatgcgg gagactccct ggactaagaa gcggaccgac ccgtcgggga
     2461 aacgcagcat tttcaaaatc aaaccggcgg tgcccactcc gtagaggtcg ttcggcgtcg
     2521 gattttcggc ctcggcgttc ttctgggcga agagcccgat gggctggccg tccatcaggg
     2581 cctcgtcgat catcttggcg tagcgctgct cggtgatcaa aagcggggtg aggaaagccg
     2641 gaaaaaccac gatcccccgc gtgggcaaaa ccggcaggga gtcgagtttg attttcggtt
     2701 cttcggtttt aaccggctct ttgaccagtg gcatccaccc tccggaaaat tcgtatggtc
     2761 aggaccgctg ttgcctattc cggctcgatt tctacggttc gggatttttc tcgattctgg
     2821 ggaagtttcc ccacccgaat ttcgagcatg ccgtcgtgca ggagggcggc cactttttcc
     2881 gggtcgcccg gctccggcag gtggacgttg cgctcgaact tgccggtggg aatttcgcag
     2941 ttgtggcagg cggcgcgttc ggcaaacttc ttctgcgccc gctcgccccg gatggtcagg
     3001 atgttgtgcg ccagcgcaat ggcgacctcc gactcctgca ccccggccag ttcgcagatg
     3061 accaccatct ctttattcag gttgaagacg tcaatgtgcg gccgccagag gcggtggctg
     3121 gtcaaaatcg gtgaggtgcg gatcttgtag aaatggtcaa agaggaggtc catctcctcc
     3181 tgaatgtggc ggatgtcccg gaaaatgtct ttgcgttctt caggcatctt tccctcttta
     3241 tgtcgtcatt tccacaggta atacgtactg tccctcccgg gtaagagtgc cttcaagtcg
     3301 tcaagcccga ccccggttgc gggcgtaaat caggcgcagc ccctccaggg tcaaggtggg
     3361 gtggactttc tgtatggtct ccgaatcttt aacgaccaga tcggccaggc cgccggtggc
     3421 gacgactgcg gcctgccggc ccagttccgc ttcgagtttg cgcacgagga aatcgacctg
     3481 tccggctgtg ccataaacaa tacccgccaa cagactctcc tcggtggttt tgccgatggc
     3541 gcgtgcgggc cgggtcaggc gcacggagaa caactgggcc gcccggcgga aaaggtcagt
     3601 cgccgaggtt tcgattcccg gggctatggc accgcccaag tattcgcctt gctgggagat
     3661 ggcgtcgaag gtcgtcgccg ttcctaaatc gacgacgatg gtcggtccgc cgtaaaggtg
     3721 gtaggcggcc accgcattgg ctaaccgatc ggcgccgacc tggcggggat cggtcgttcg
     3781 cactttgatg cctaacttca ttgcggcgct aacggtgacc ggctgcacgc cgaagtactt
     3841 ctgcgccatt tcttcgtaga tcggggtcaa aggcggcacg actgacgcca gacagatccc
     3901 gccgggcttg atcggtctct tggcatggtt tgcgagcagg ccggtgacca gaatgccgca
     3961 ctcatcggcg gttaaagcat ggttcgatgc cagccggaaa tgcgcccgca gccgttcccc
     4021 ggcaaagacg ccgatgacgg tgtgggtgtt gccgatatcg atggcgagga ggtgactggt
     4081 ttgctttccg cgcacaaggg gtaaagtatc cagaatgggc cgacctgtca aaatgcggtc
     4141 agatacccag ctatttcaac tttattggct ggaaaatttc ctgatgcgta tattatcaat
     4201 agaggtatgt tacgttcagt tgggcgagac ggttcgctcc cggtttgacg aaggaaaaaa
     4261 ccatgtggaa gaaaaaatca accgcagctt tcgttacggt cgcggtttct attctactca
     4321 tctccatcgc cttcgctcag cggcctttca cagtgaaaga aaagcaactc tactacggta
     4381 ttaaggctgt ggctgctccc gaacagttta agcaatttga aaagctatct ggaagggagc
     4441 gagcggaatg gctgcgaatg ttctgggcac ccaaggatcc cacaccgacc acgccagaaa
     4501 atgaatttta cgaagagcac cagcgccgcg tagctcacgc ggtagaaatt ttttgcactg
     4561 cacggccagg accgccttgg gatgaccggg gcgagattta tattcgctat ggggaaccgg
     4621 atgagcggaa tttccgcgta gagcggcatt acagtcgtca gatggcagcc cactcggcct
     4681 acgagctccg ggatgtgatc agcgatccgc tcaagtaccg gtcgcttgta gctcatcggt
     4741 tagccaaccc ggaaagagac tttggtggtg atatcagtga tgagccctct gcatcgatag
     4801 cccgtggttc cttcaccctc cgccctcgcg atgaggatct cctgagagat cccaaagccg
     4861 aatttttaag cactcgcctc gaatttgcgg cagtttcggg agaggtgtgg cggtactacc
     4921 gctatggttt gacttttcaa ttccaggatg agcagaattt agggtacttc ggactggtgc
     4981 cgtacatcga cgccttcggc acggcgcagg actaccagca attcgtgcgg caggcggtgg
     5041 tgtcgctcga tgtgcagaaa gagatttatc agcacgacta cggcggtaag gcgatggact
     5101 atgcttttac ggtgatccgc ttccgggcac cgaacaaggt gaattacgtc gtggacgtga
     5161 acctgggatt gccgttagag aaattgggtg tggggggaga ctccgtcacc ggcgaaaaag
     5221 ttgaatgtat gcgccggatt gtgctttacg atgaaaacta ccagccgctg gcaaccgaca
     5281 gcactacgct cgctgcgccg gtaagtgccg atagccgcaa gggacggttg ctcttggagc
     5341 agaagcgcta cgaactgaaa ccgggcaggt atatcctggc tgttgaagtg cgcgatttgg
     5401 ccacccagaa gatcggtatc tacaaaaaag aactgctctt gccggagtac atcgcaccgg
     5461 acgtgcagga gatttcggac gtggagttgg cgagccatat tcgcccggcc aacgagtggg
     5521 ataaaaagta ccggcacaaa gaactggtgg tgatgcccca gccctcgcgg gtgttctttc
     5581 ccgaccagac gttgcagtat tactttgagg tttacaatct caaaaaggac aaggacggca
     5641 aggcgcggtt ctcagttaca ccggaagtgt acggctaccg cacccgtaag gtggagtaca
     5701 gcttcaaccc gcgcgacttg gaagccgaca cgaccgacat ctaccagacc ggcaagctca
     5761 acttgcgcga tttaactccc ggagagtaca ttctctccct caaggtgaaa gatcggataa
     5821 ccggaaagga gaagacggcg ctggcggggt ttaaggttgc aaagagcgaa tagtcatcgg
     5881 ttacgtaggc gcggcgccct cgccgcgctc aatctgcggg tggcgaccgg gggcggtcgc
     5941 cccacgcggg agaaaagatt cgacgacgct ctggagaacg tcgctacaca cttaagatca
     6001 atgggtgggt aggagtccaa cgagcagagg aggcggaatg tccaacggca cggggcttac
     6061 caaaaaacag atggtgatgt ttgcggttct gatcggcgtg gccgtgttct taggcgtcta
     6121 cttcggcatt tacttcgggt cgcggggcct cgggcggcac ggcggcgaag cgccggtgga
     6181 ggcgaaattc aaggccaatg atccgctgcc agaagtggcc gtcctcgacg tgaacgggac
     6241 gcttgagccg ctttcgaaaa ggatttccgg aaaaggtgca gtagtgatgt tcatgtctct
     6301 gggttgccac ccctgccagc aggaatcaga aaagtggcag aagattctgg cggagaaaca
     6361 tcccgaggag tttgccgtgc tgggcgtctg ctacgataaa accgagcaaa ttcaagccta
     6421 cctgaaggag cacggcttta ccttccctat tttctgcgac tcggcgcggc ttttcatgcg
     6481 cgactggagc gtggcgcagt tccccacggt ggccgtcgtc aatcgcgagg ggaaaattgc
     6541 gaaagtctac tccggtttcc atcccgactt tgacgataaa gaagtgaaga aggttctgaa
     6601 gacgtaggtt gcatcggggg ccccgcaaaa agtcctccgg cgagtgaggg aggacttttt
     6661 tgttgcgggg ctggcgcata tattccgccg ccatgcgctc ggctctcgtt ttcttcttct
     6721 gcgtctggat cggatcgccg gccctatcca cgccatctcc ttctcggcga gtctcgaaga
     6781 gtcgagtctc cgacgagtct gcgacaaccg ggccggagca aacccgccag aagctcgtcc
     6841 gcattgccgt cctcgaagac cagggctggc tggggtatgg ccaacttttg cgctacctga
     6901 aggacgccga gcccgtcgtg cgccagcggg cggcgcgctc ctgcgggatt atccaggact
     6961 cgctggcctg cccggttttg atccaagcgc tcaaagatcg ggattctacc gtgcggcagg
     7021 aagccgcctt tgccctcgga caaattcagt gccgggaggc caaaggggca cttttgaagg
     7081 cgacgcggga tcggtcgccg acagtccggc agctggcgct ggaagcgttg gggaaattgg
     7141 gcgatacgac ggtcgttgac cggattgcca aattcttgag cgaccccagt tccgccctgc
     7201 gcggggaagc ggctctggct ttggctcgac taaaaagtca ccaagccatt gcaccgctgg
     7261 cgcgggccgc gcgggattgg gaccccgccg tacgctggaa agcggtctac gcgctcttcc
     7321 ggcagaacga ttcggtgacc atcccgatct tagaagcgaa atttacggac aacgacccct
     7381 acgtgcgggc ctttgcggca cgcgggctgg ggcagttgca cgcttcggcc ccggcgatgg
     7441 tgcccttgct ttctgatccc gactggcgcg tacaggtgca ggcggtgcgc gccctggggc
     7501 agagcaaggc gtccgaaagt ctgcttttct ttttggagta tgaccgccgg gaaggaacag
     7561 gaccgctttc caatccctac ctggtcgggg aagagctgac cgcactgggg agtgttcaca
     7621 gtgacagtgc ctaccaggtg gtacgggcct tcctcgccag tccgattccg gacctccggg
     7681 cgcgcgcgac ggcggctttg gcgcagatcg attcggcccg cgcgctggaa gacctcaagc
     7741 cgttacgatc tgacaccaac tggcatatcc gcgcgcaagc ggcggaagcg ttcggcgcca
     7801 tccgcacgcc ggaggccttt gcgctcttag agtcgctctt cgtcgatccc gactaccgcg
     7861 tccgctaccc ggcgctggag gcgatgggga agttccccac tgaacgctgg gagccgtttg
     7921 tcaaacgggc cctgggggaa gatgagcagg cactcatcgc caccgcaagc gagatcgcgg
     7981 gcaaggcgca cgattcgacc ctgctgaacg acctcgtcgt ggggttctgg aagcaccgga
     8041 tggatcggga gaccgacgcc aagatcgcca tcctcgaggc cctggagcgg ttcgtcgatg
     8101 ccggccgcct atccggcacc atcgcccaag cgctcgactt cggcctgcag gacaccaacc
     8161 gcctggtgcg gcagctggcg gtgaaaatct tccggaaagc cggtctggaa cgggggggct
     8221 tcaccggaac gtttgtcagc gatatggatg cggagaaata tcgcgccgtg tacgagcagt
     8281 acacgaccaa tccgaaagcg gcaatcacca ccggaaaagg gataattgtc ctggaacttt
     8341 tgccccggga ggcgccccgc acggtggcga atttcatcaa actggcgcag acaggttttt
     8401 acgacaaccg cgtctggcac cgggtcgttc cgaacttcgt cattcaggac ggctgcccgc
     8461 gcggggacgg ctggggcggg ccggggtaca ccatccgctg cgagtacaac aaactccact
     8521 acgagcgggg gacggtgggg atggcgctct ccggcaagga caccggcggc agccagtact
     8581 tcatcaccca gtcgccgcag ccccacttgg acggccgcta caccattttc gcgcgcgtgg
     8641 ttgaagggat ggaggtcgtg gacaaaatca tggtcggcga caaaatagag aaggtcacca
     8701 tcatcgaata accgtagtga ggagagcaca atggaattga aaacggtgcg gctggccatg
     8761 cctaaagagg cgaatgtcat tttcggccag acccacttca tcaaaaccgt cgaagatctc
     8821 tatgaaatcc tggtggggat gctccccggg atcaaattcg gcttagcctt ctgtgaagct
     8881 tccgggccct gtttggtgcg ggcggagggc aacgacggcg aacttaaggc tgctgccatc
     8941 gccggcgccc agcaggtggc ctccggccac acctttgtca tcttcctgcg gcaggctttc
     9001 cccattaacg tgctcaatgc catcaaaaac tgctccgaag tttgctcaat tttctgcgcc
     9061 accgccaatc cggtggaggt cgtcatagcc cagaccgaac aggggcgggg ggtgctgggg
     9121 gtgatcgacg gcttcccgcc caaaggggtc gagagcgacg atgacctgac gcgccggagg
     9181 gagtttttgc gtaagatggg ctataaaaag tagccagtag ccaggggcca gtagccagtc
     9241 accagagaga aaaaaccagt agccaggggc cagcagccag ttaaaccaat ggcagggttt
     9301 cgagatttga aggtgtggca aaaagcgttc accctgtcgg ttgaagccta ccgaataacc
     9361 aaaaacttcc ctgccataga gcaatatgcg ctttccagcc aacttacccg agctgcggtt
     9421 tccgtgccgg ccaatatcgc cgaagggtat gaccgaacga gcaaaaaaga atacaggtat
     9481 tacgtttcca ttgccaaagg ctctctggcc gaagtcgaaa catattttct tctggcaatg
     9541 gaattaggtt acattacacg cgaggatttc aataaaatcg attcactccg cgccgaggtc
     9601 gggcgcattc tcacagggct gctgaaaagc atatgaggtt tctgggaaaa gaactatttc
     9661 gtgagccgtc tatcaagacg tgctggcccc cgggcttttt tatttttctg gctactggct
     9721 actggcccct ggctactgtt atctggctac tggacgcttc ctttggattt tgacagcgac
     9781 ctcaagatcc ccggccacga cttctactta gacgcgccgc gcgcggtgcc gtgctctttt
     9841 gtctcccacg cccactccga ccacgtccgc cggcacggga aaatggtcgc cacgccggag
     9901 acgatcctgc tctgccggct ccggttcaag tccgcctcgg gcggacgggc ccgggcggtg
     9961 ctcttcggcc agccgaccaa aatcgacgat cttaaaatcg aactcttccc ggcgggacat
    10021 atgctgggag cggcacagat tctcgtccag ctcgacgatc aaaaaatcgt ctacaccggt
    10081 gatttcaatc tccagccggg ctacacctgc cccggcgcgg aggtcaagcc ctgcgacgtt
    10141 ttaatcatgg aatcaaccta cggccggccc cactaccgct ttccggagcg ggagaaggtg
    10201 gcgcagcaga tttgcgcttt catcgatgcc gccttcgcca gccggaaaat tccggtgctt
    10261 ctggcgtatg cggtgggcaa ggcgcaggag gtgatgaaac ttctgggcga ccggggctac
    10321 aagatggcag tccacaagtc aatttttgac gtggcccgag tttaccagcg ctgcggggtg
    10381 gaactgacga actatgatct cttttccgat ccgctgttac ctgagaccgt tctcattttt
    10441 cccccgtttt tctggaaact gccgccggtg gcaaaaatcg aaaaccggcg cgtcgccttc
    10501 ctctccggct ggggcattga cccccaggcg aaacaactct atcatgccga cgaagttttc
    10561 cctttctccg accatgctga tttcgccgac ctcgtcgctt acgttcaaaa ggcgaaccca
    10621 aagaaaattt acactctgca cggtttttcc gaattcgccg agacgttgcg gagaatgggg
    10681 ttcgatgcgg agtatttgcc cgaatccccg gtgagaaaag ttccactcga agctgccagt
    10741 ccccaggagc ctgtcgccga gagtgatctc ttcagcacct gaaggcaggc aacgcttgac
    10801 acgttttccc ccggaacgta ggtttggtgc actttgaacc ccacctctga ggaggccttg
    10861 tggctcaggt cacggtagaa agccgacccc aaccgaccct cgcccagagt taccagccgt
    10921 acatcaaagc gtccgagaac gtgtcggagt tcacccttcg ggcgctcatc ttgggttcga
    10981 ttctggcctt ggtcttcggc gtggccaacg cctatctggg cctgaaagtc gggatgacgg
    11041 tatccgcttc cattccggcg gcggtgatgt cgatggcgat tctgcgcggc gtgttgaaga
    11101 ggggcacggt gctggagaac aatatcgtgc agaccatcgg ctcctccggc gagtcgctcg
    11161 ccgccggagt gattttcacg atccccgcct tcctcatctg gcaggcctcc gagccggagt
    11221 tcactttcaa aattgccaac tggcaaattg tggttttgtc gattctgggc ggttttctgg
    11281 gcattctctt catgattccg cttcgccggc atttgatcgt caaagagcac ggtatcctgc
    11341 cctaccccga gggcaccgcc tgcgcggaga tattagtcgc cggtgacgaa ggcggttcca
    11401 aagcgcggac cgtgttcgcc ggcctgggaa tcggcggtct gtacaaagcc ctgatgtcgg
    11461 gtggcaaact ctggccggac aattttcact tcacgcccgg gttcttcaag gggtcgcaaa
    11521 tgggcgttga cgcgacgccc gctttgatgg gggtggggtt catcatcggg ccgcggattg
    11581 ccgccttgat gttcgccggc gccgttctgg gctacgtcgg catcgggccg cttttagcct
    11641 ttctcggaaa ctatctcacc acgccggtgc cgccggccac agttcccatg gctcagctct
    11701 caccggcgga actgcggctc tactacatcc gctacatcgg catcggggcc gtggccatgg
    11761 gtggcttcat ttcgcttttg cggtcgttcc cctccatcat ctcctcgttc aagcagggaa
    11821 tcggtgactt tgcggcccga ggggcaaccg ctaccgtgcg caccgatcaa gacctgtctt
    11881 tgaaattggt cctgggcggg tcattcttgg tagccgtggc gatctggctt ttgccgggca
    11941 cgcagatgca cttcgtcggg gcgcttctgg cggtcttgtt cgggtttttt ttcgtgacgg
    12001 tttcctcccg gatggtgggc atcgtcggct catcctctct gccggtttcc ggcatgacca
    12061 tcggcacgct catcgtcgcc tcggttatcc ttttggcgta cggcgtctcc ggcacggccg
    12121 gcatggtggg agcgatgatg gtggggacgg tggtggcaat tgcggtctcc atgtcgggcg
    12181 acgcgtctca ggatttgaaa accggttttc tcgtgggagc cacgcccaaa aagcagcaga
    12241 tcgccgaatt tgtcggcgtt ttggtgccgg cggttatcat ggggtacgtc ctgctattct
    12301 tgaacaaaac gcacggcttt gtggggccgg gggccttcga agcgccgcag gccaacgcga
    12361 tgtcggcggt ggtcaagggc gtaatgaccg gcaacctccc ctggattttc gtcatcgtcg
    12421 ggacgatggt gggcctctcc atcgaacttt tgggcgtgca cgccctgccg gtggccatcg
    12481 gtttatacct ttcacccgac ctttcgacgc ctatcgtcat cggcagtctg atttaccttg
    12541 cggtgcaaac cttctccaat ggcggtctga aaaacagccg cattgaaaaa ggaattctct
    12601 tcggctcggg gctggtcgcc ggagatgcct tggtcggcgt gggaatcgct tttctggtgg
    12661 gatcgatctc ttcctacgaa gcgttcttcc gccgtgagga taacatcggc acctggctgg
    12721 gcggctacgg gcagatcttc accctgggta ttttcctgct cttggggctg ggatatttgt
    12781 accacatctg gcggatgaaa gcgcgcgcgt aaccgaacgc agcaggtttc gggtacctaa
    12841 gaggaaagat gacctccaac gagttaaaac acttcctgga gcaatgggag cgggagtacg
    12901 ttacaaccgt taaggtgctg caggcgatcc cggacgaccg actggactat aaaccggctg
    12961 agaaaagtcg ctcgctccgc gacctgatgt ggcacatcgt gggcgtggaa aattttgccg
    13021 ccaccttggc tttgaccggc gcttacccga aagagcgact gcctcccccg gccaccgtta
    13081 aagaactcct tgaattttat gaacggcagc acaaggcgtt ggtgtcgcaa gtcgctggtg
    13141 ctgaggccgc gctcctgagt aggaccgtgg actttttctc ccggccagtg ccggtttccc
    13201 acattctctg gcgggtcctg ctctaccacc agatccacca ccggggacag ctctcggttt
    13261 acctgcggct ggtcggcgca aaagtacctt caatttacgg cccgtcggct gacgaatcgt
    13321 agaagaaggt tattaaacca aggagattat gagggagact tacggctgaa cccggtacgt
    13381 gccggcacgg gaaagatttt tgaaggtcgc tttcgttttg atgatgttcg ccgttcccaa
    13441 ctgattccag acagggttct ggtactccaa gaggcggagg gtggttccat tccggagtcc
    13501 cttcgtttgg tgcgtgagcg tggcaggctt ggcaaaggtg aggtgcgccg ggagaatcac
    13561 gtagtcggtc gactgccagg aactatcagg ggccagtgtc ttctgcgctt tgatcgttat
    13621 catcaccttg ctggtgaggg cgccgtgtgg aatttgtaaa acgctcgtgt cctggcccag
    13681 agggatgaga attttactac cgcctttccc gacgatgcgg ctcacctgag cggacagaaa
    13741 ggtcaaacta tctccggtgc catcgtcttg gtagggttgc acctgaaaat agggcggcgg
    13801 cgaggcggct tccgaaggaa tcttgtcagg agcaagagtc gccggggctt cgaccggccc
    13861 cgtgggtgat ttcttggtgc agccaaccag accccaaccg accaccaaaa tgatgccgcc
    13921 cacctgcaaa gccgcttggg cgattttcct tgtttttttc atttgctctt tcatttcacc
    13981 tactgatcac tgcgaacgca atgccaaccg gacggaccgc aggaaaattg cgctactgct
    14041 ttcggagcaa gagattacgg ggatggattg gaattgtgca ataattgaat cgctgctaag
    14101 aatctggaca atcccatact ttatggcatt tcgccaacag cccctcttgg cttgactggt
    14161 gccacccaga gaaaaagatt tgatcttgag cgcccttact ttagatgcca gagatgccag
    14221 ccgggccggc ggcggactct cctcaaaagt tgaaccgggt tatttctacc cccgggggtg
    14281 tttgacaaat tgaccattat gagctatttt ggtttccgat tattgtagag aacggcctca
    14341 gtggctaagt ctgaggaggt gaaaagtatg cccgcgaaga aagcgaagaa atcgaccaaa
    14401 gcccggtacc aatgccaggt ctgtgactac aaatcttcca agccgatgat gtgttgcggc
    14461 aaaccaatga agaagatgaa gtagccctcc ggcaaagcag ttcctgcggg tcggtacgac
    14521 tccttccgag agggttaccc tcccggtttt ttagagaaac cagttgacgc gccccgcagc
    14581 gcatattaga cagcgatggc ggaagtgccg tgcggccggt ttgcctccgg tgcagtggga
    14641 ctcctttgga gcaacggaca agcccgcgcg gggaaaggtg gtgaggtgat gagggtaaac
    14701 ttttttttga aagtattcgg gacgattttc ctcctcttgt tcaactccgc actccgggcg
    14761 caggagacca gtttttccac cacccataac tacgagcttc ccggcccttt cggcgtggcg
    14821 gtagcggatt tgaccggtga cgggcaggtg gacatcgccg tctccacgac ctcgggggca
    14881 atcggtctgg taaatttcgg ccagcagcag ttcgttcccg cactcacctt ctccggaaca
    14941 aacctcggga atcttgcggt ggcggatttg gacggcgacg ggcgtctcga tctggtggct
    15001 cccgaccgga acgaatcggt catcgttttc ctcgctaccg gaccgggcca gtttgccccg
    15061 ggcgtggcgc atgccgtggg gtcggtgccg acccaggtgg cggcatccga tatcgacggg
    15121 gatacctatc ccgacttgat tgccaccgac tttggcgaca atacgatctc gattctgaaa
    15181 gactacggcg atgcggtcta cggcccgccc cacgtgtttg ccgcggggca aaaaccttac
    15241 gctctggttg cgactgattt tgacggcgac gggcgccccg acatcgcggt gaccaaccag
    15301 ggctcaaata ccgtctcaat tttcaccaat gtcggcgggt acgcatggtt tgctccggcg
    15361 gttgcttacg ccgtcggcct tgggccggcc ggtttggcgg ccgccgattt caacggcgac
    15421 ggcgcacccg atctggccat agccaacagc gccagcgatg acattgcgct cttgctcaat
    15481 aacggggacg gcacctttgc cccggcccgg tatttccccg ccggggacag cacctgggcc
    15541 ctgacggcag tcgatctcga ccgggacggc gacttcgatc tggtggccgg ttctctaggc
    15601 agccacgaac tggggctttt ctccaac
//
LOCUS       DBNGZA010000289         1703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1535809, whole genome shotgun sequence.
ACCESSION   DBNGZA010000289 DBNGZA010000000
VERSION     DBNGZA010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1535809"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1312)
                     /gene="rlmN"
                     /locus_tag="AC16FU_06240"
     CDS             complement(233..1312)
                     /gene="rlmN"
                     /locus_tag="AC16FU_06240"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR2994005.1"
                     /translation="MKKLQPLNLIGLWLPELEELVERLGERPYRARQLAQWIYKRGMT
                     DFERMTDFAKLFRQRLAEVATCERLVCQSTLESPLDRATKYLFELADGERIESVRIRM
                     NEKITLCLSSQVGCPLACTFCATGDMGFRRNLTAGEIVSQVLQLREDLKEQKINNVVF
                     MGMGEPLLNYEQVVKAIRIMTSELGCELPARRITVSTAGWVPGIYQLAEEKLKVKLAV
                     SLNAPDEERRKKLMPVTRKYSLDELLKAAKHFAFRTRRRVTFEYVLMEGVNDNPADAH
                     LLAKLVRGIPCKINLILYNPNKGQPYIRPAPERVERFREILYPRAPAVTLRISKGWDI
                     QAACGQLAADYPLRKQAGRKVSLAV"
     gene            1512..>1703
                     /locus_tag="AC16FU_06245"
     CDS             1512..>1703
                     /locus_tag="AC16FU_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994006.1"
                     /translation="MRKVLLGSICFLALPIILFAYSFGPPPGFTGAPGELSCTFCHIG
                     APLNPGDATLSVIGLPTDYQ"
BASE COUNT          363 a          476 c          472 g          392 t
ORIGIN      
        1 taagccgaag gttactaaaa ccagagtacc caggcctata gtcaggaaga aaaacttttt
       61 cattgcggct ccattgatta gctctcagga tcagttaccg cgtcgctgac ggacaacacc
      121 cgcgaggcca cgctactcac tttgttaata actgataatt atacgtgaga tgttcccccg
      181 atttctcaag aacttctttc gacagccttc ccgcctgcag cgaaaagagc agtcacaccg
      241 cgagggagac tttacgaccg gcctgtttgc gcaacggata atcagccgcc aactggccgc
      301 aggcagcttg gatgtcccac cccttgctga tgcgcaaagt caccgccggc gcgcgggggt
      361 agagaatttc tcggaaacgc tccacccgct ccggggcggg gcgaatgtaa ggttgccctt
      421 tgttcgggtt gtaaagaatg aggttgattt tgcagggaat cccccggacg agtttcgcca
      481 aaaggtgggc gtctgcgggg ttgtcgttga ccccttccat caagacgtac tcgaaagtga
      541 cgcgccttcg ggtgcggaag gcgaagtgct tggcggcttt gagcagctcg tctaacgaat
      601 acttgcgggt cacgggcatc agtttcttcc gccgctcctc gtccggagcg ttgagcgaga
      661 cggccagttt cactttcagt ttctcctcgg ccaactggta gatacccggc acccacccgg
      721 cggtggaaac ggtgatccgg cgtgcgggga gctcacagcc caattccgat gtcataatgc
      781 ggatcgcctt gaccacctgt tcgtaattga ggagcggctc gcccattccc atgaagacga
      841 cgttattaat cttctgctcc ttcaggtcct cgcgcagttg caaaacctgg ctcacgatct
      901 cgccggcggt cagattgcgc cggaacccca tgtcgccggt ggcgcagaaa gtgcaagcca
      961 aagggcagcc gacctgggag gagaggcaaa gggtgatttt ctcgttcatg cgtatcctga
     1021 ccgactcaat tctttcgccg tcggccagtt cgaacaggta cttcgtcgcc cggtcgagcg
     1081 gtgattcgag cgtgctctgg cagacgaggc gttcgcacgt cgccacctcg gccagccgct
     1141 ggcggaacag cttggcgaag tcggtcatcc gctcgaagtc ggtcatcccg cgcttgtaga
     1201 tccattgcgc taactgacgg gcgcggtagg gacgctcccc caggcgttcg accaattcct
     1261 ccaattccgg cagccagagg ccgatgaggt tcagtggttg tagctttttc aaagtcaaga
     1321 ccatactaac gtaatatacg accgcgaaaa tgctgaaaaa gggcaacccc ggcgaaaata
     1381 ttagcaacaa tttgacagtt cttttgttat aatagttcgg tgattctctt ccctgcgaaa
     1441 agcgtcgccc cgtgagaagg gtgagcgcga gtcgtaagat tcggaaggtc gtaaaatcga
     1501 gaggtgaggt aatgcgaaag gtcttactgg gttcaatttg ttttctcgcg cttcccatta
     1561 ttctttttgc ctattcattt ggtccacctc cgggtttcac gggtgctcct ggagaactat
     1621 cgtgtacgtt ctgccacatt ggagcaccgc tcaatccggg ggatgcaacc ctctccgtta
     1681 tcgggctgcc gaccgattac caa
//
LOCUS       DBNGZA010000290         4388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1854667, whole genome shotgun sequence.
ACCESSION   DBNGZA010000290 DBNGZA010000000
VERSION     DBNGZA010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1854667"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16FU_06250"
     CDS             complement(<1..529)
                     /locus_tag="AC16FU_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR2994007.1"
                     /translation="MNTTATAAAGKIAPANERTLRRDTWWLQPLLTVIGLGGFAIYAT
                     WVAFQNANYLVEPYLSPFYSPLIKVSWWSFSPAFLVLWAPLGFRATCYYYRKAYYRSF
                     FWDPPACAIGEVWKGTYHGETRFPFILQNLHRYFFYAAVVVLAFLWYDTIKAFIFDGR
                     FGIRIGSLLYLVNIIL"
     gene            complement(629..817)
                     /locus_tag="AC16FU_06255"
     CDS             complement(629..817)
                     /locus_tag="AC16FU_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994008.1"
                     /translation="MAKKQSFADKASKVKHTVTCPVCHSAQTPTLYIKSEQSPGGAWK
                     FRQVHVNVCKCNQAEVMG"
     gene            complement(929..2938)
                     /gene="uvrB"
                     /locus_tag="AC16FU_06260"
     CDS             complement(929..2938)
                     /gene="uvrB"
                     /locus_tag="AC16FU_06260"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR2994009.1"
                     /translation="MAQFELVSDFPPSGDQPKAIDQLAEGLQNGANHQVLLGVTGSGK
                     TFTIANVIARLGRPTLVISHNKTLAAQLYGELKGFFPRNAVEFFISYYDYYQPEAYVP
                     QTDTYIEKDTSINDDIDRLRLRATSSLLEREDVVIVASVSCIYGIGSPAEWKEQMLLL
                     EVGETVERDHLLRRLIDIHYNRNDFEFSRGTFRVRGDVVEVIPAYEEHALRIELFGDQ
                     IERISVIDPLRGEIAAEKKKAAIYPAKHFVTTQPRLEAAIKNIEAELHERLAHFRSQG
                     KLLEAQRLESRTKFDIEMMKEIGYCTGIENYSRHLSGRKPGERPFCLLDFFPKDFLLV
                     IDESHQTVPQIQGMYHGDRSRKEVLVEYGFRLPSALDNRPLTFDEFEALADRVIYDSA
                     TPAEYEIKKTRGVVVEQIIRPTGLIDPKITVKPLDKQVDDLLEEIRARAASHERVLVT
                     TLTKRMAEDLADYLAQMGIKVRYLHSEIEAIDRVEILRNLRLAEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLIQIAGRAARNKAGEVIMYADAVTDSMRKAIDET
                     DRRRKLQEEYNRTHGIEPQTIYKTKEEILRATAFADSRTAAVPGEPVEKLGFLEKMEL
                     EDQLAYLTKEMKKASGTLDFERAAAFRDKIAQLKESLKKKTKPVR"
     gene            3113..3625
                     /locus_tag="AC16FU_06265"
     CDS             3113..3625
                     /locus_tag="AC16FU_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR2994010.1"
                     /translation="MKFSLHAKDDPHRLLHLEAAPDLLHIAYGEVRFARPVVCDVSVD
                     KRGDAFVCHGEIRTVVGLDCDRCATPFDHRVGAAIDLVIQRVTGGPAKDELDADYVEL
                     PPHVSEYDLSDRVRDAVILALPIHPLCQAGCRGLCPTCGRNLNSGSCNCRPESSDSRW
                     EDLKKLKYSL"
     gene            3635..3817
                     /gene="rpmF"
                     /locus_tag="AC16FU_06270"
     CDS             3635..3817
                     /gene="rpmF"
                     /locus_tag="AC16FU_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR2994011.1"
                     /translation="MPLPKRRHSKTRGAKRRTHWKLEPPAVVECSHCHQPKVAHRVCP
                     HCGYYDGKEVIAPEEA"
     gene            3824..>4388
                     /locus_tag="AC16FU_06275"
     CDS             3824..>4388
                     /locus_tag="AC16FU_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014554.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR2994012.1"
                     /translation="MSAPVKIAVDAMGSDHGQEAVVAGAVLALREKTNGSRRSQPVPT
                     ELILVGEEKQIRRELGRLKAEDLPIQIAHASEQVQMEEQATEGWKKKDSSIAVAMRLQ
                     KERQVQAVVSGGNTGAVMTTALLTLGRLQGVIRPAIASLFPTLRHPTVILDVGANADC
                     KAQHLQQFGVMGSIYYSHLLKRAEPRVG"
BASE COUNT          931 a         1213 c         1324 g          920 t
ORIGIN      
        1 gcaggattat gttcactaaa tagagcagtg acccgatccg aatgccgaag cgaccgtcga
       61 agatgaaagc cttgatcgta tcgtaccaga ggaaggccag gacgacgacg gcggcgtaga
      121 agaagtagcg gtggaggttc tggagaatga agggaaagcg agtttcaccg tggtacgtcc
      181 ccttccacac ttcgccgatc gcacaggccg gagggtccca gaagaacgaa cgatagtacg
      241 ctttgcggta gtagtagcag gtggcccgga aaccgagcgg cgcccagagg acgaggaatg
      301 cgggcgaaaa agaccaccag gaaactttga ttaagggcga gtagaagggc gaaaggtacg
      361 gctccaccag ataattcgcg ttctggaaag ccacccaggt tgcatagata gcgaatccgc
      421 ctaatccgat gacggtcaaa agcggctgaa gccaccaggt gtcccggcgg agggttctct
      481 catttgcggg agcgattttt ccggctgctg cggttgcggt cgtattcatc gccaaggtgc
      541 aatcgtattg tgccaaataa agtttgcgcc aaggaattgt caataaaaaa agcggcctta
      601 cggccgcttt ttcaacccaa tcactctttt atcccatcac ctcggcctgg ttgcacttgc
      661 agacgttgac gtgcacctgg cggaacttcc aggcgccgcc ggggctctgc tccgacttaa
      721 tgtaaagggt cggggtctgc gccgagtggc aaaccgggca ggtgacggtg tgcttgacct
      781 tggatgcttt gtccgcgaac gactgtttct tggccattta agcctccgtt cttctactca
      841 agtatacgta gaaacatttg ggttgtcaaa gagccggaca acgatttttg accggagaag
      901 agaaacctcc cgtccgagag aacgcggatt atctcaccgg cttcgttttt ttcttcagtg
      961 attctttcaa ctgcgcaatt ttgtcccgga aggcggcggc gcgttcgaaa tccagggtcc
     1021 cggatgcctt cttcatctct ttggtcaagt aggcgagttg gtcttccaat tccatttttt
     1081 ccaggaaacc aagtttctcc accggttcgc ccggaacagc ggcggtgcgg gagtcggcaa
     1141 aggcggtggc gcggaggatt tcttctttgg tcttataaat cgtctgcggc tcgatgccgt
     1201 gcgtccggtt gtactcttct tggagtttcc gccgccggtc ggtttcgtcg atggctttgc
     1261 gcatcgagtc ggtgaccgca tcggcgtaca taatcacttc cccggcctta ttgcgggcgg
     1321 cgcgaccggc aatttgaatc agcgagcgct cggagcgcag aaaaccttcc ttgtcggcat
     1381 ccagaatggc gacgagagaa acctccggca aatccagtcc ttcccgcagg aggttgatcc
     1441 ccaccaggac gtcgaactcg gccaagcgca ggttgcgcag gatttcgacc cggtcgatgg
     1501 cctcaatctc agaatgcagg tagcggacct tgatccccat ctgcgccagg taatcggcca
     1561 agtcttcggc catccttttc gtcaaggtcg tcaccaggac ccgctcgtgg ctggcggccc
     1621 gggcgcggat ttcttccaga aggtcatcca cctgcttgtc gagcggtttg acggtgattt
     1681 ttggatcgat caagccagtc gggcggatga tctgctcgac caccaccccc cgtgtctttt
     1741 tgatttcgta ctcggccggc gtggcggaat cgtaaattac ccggtctgca agcgcttcga
     1801 actcgtcaaa ggtcaagggc cggttatcca gagccgaggg gaggcgaaag ccgtactcaa
     1861 ccagaacttc tttccgcgac cggtcgccgt ggtacatccc ctgaatctgg gggacggtct
     1921 ggtgcgactc atcgatgacg agcaaaaaat ctttggggaa aaagtcaagc aggcagaagg
     1981 gacgctctcc gggtttccgt cccgagaggt gccgcgaata gttctcaatc cccgtgcagt
     2041 agccgatttc cttcatcatt tcaatatcga acttggtgcg ggactccagg cgctgggctt
     2101 ccaaaagttt cccctgagaa cggaagtgag ccaggcgctc atgcaattcg gcctcgatgt
     2161 ttttgatcgc tgcttcgagg cgcggctggg tggtgacgaa atgcttggcc gggtagatgg
     2221 cggccttctt tttttcggcg gcaatttctc cgcgcagggg atcaatcacc gaaatccgct
     2281 caatctggtc gccgaacaat tcaatgcgca acgcgtgctc ttcgtaagcc gggatcacct
     2341 ccaccacgtc gccccggacg cggaacgtcc cgcgggagaa ttcgaaatca tttcggttgt
     2401 aatggatgtc gatcaaccgg cggaggaggt ggtcgcgttc cacggtttcg cccacttcca
     2461 ggagcagcat ctgctctttc cactctgcgg gcgagccgat gccgtaaatg caggagaccg
     2521 aggcgacgat taccacgtcc tcgcgctcca gaagcgaact cgtggcgcgc aggcgcagcc
     2581 ggtcgatgtc gtcgttgatc gaggtgtctt tctcgatgta ggtgtcggtc tgaggcacgt
     2641 aggcctccgg ctggtagtag tcgtagtagg agatgaaaaa ctccacggcg ttacggggga
     2701 agaacccttt cagttcgcca tagagctgtg cagctaaggt cttgttgtgg gaaatgacga
     2761 gggtgggccg gcccagccgg gcaatcacat tggcaatcgt gaaggttttc ccggagcccg
     2821 tgactcccaa aagcacctgg tgattggcgc cgttttgcag tccttcggct aattggtcaa
     2881 tcgccttggg ctggtcgcct gagggcggga aatcagaaac gagttcaaac tgagccataa
     2941 ggtaagtgat aagaatcggg cataacagtc gcctgttaat aaatatacgt acaaatattc
     3001 ttagaacaaa gagatgaagg tcgcaagtta agagagttga tagagttacc aacccggact
     3061 gcgccgggaa ggagcttgac aaccgcactg ctgcaacgta tcctgtttgc ccatgaaatt
     3121 cagccttcac gcgaaagatg atccgcaccg cctgttgcac cttgaagccg cacccgacct
     3181 gctccacatc gcctacggcg aggtgcgctt tgcccggccg gtggtctgcg acgtgagcgt
     3241 ggataagcgc ggggacgctt tcgtctgtca cggcgagatt cggacggtcg ttgggttaga
     3301 ttgcgatcgg tgtgcgaccc cttttgacca ccgggtgggg gcggccatcg acttagtcat
     3361 ccagcgggtg acgggcggtc cggccaaaga tgagttagat gccgattacg tggaactgcc
     3421 accgcacgtc agtgagtacg atctctcgga ccgggtacgg gatgcggtca ttctcgcctt
     3481 gccgattcac cccctgtgcc aggccgggtg ccgggggctt tgcccgacct gcggccgcaa
     3541 tttgaattcc ggttcctgca actgccgacc ggaaagctcc gactcgcgct gggaagactt
     3601 aaagaaactg aaatattcac tctgaggaac cagtatgccc ttacccaaac gccgccactc
     3661 gaagacgcgg ggcgccaaac ggcgcacaca ctggaaattg gagccgccag ccgtggtgga
     3721 gtgttcgcac tgtcaccagc cgaaggtcgc gcaccgggtc tgcccccact gcggctacta
     3781 cgacggcaaa gaagtcatcg cgcccgaaga ggcgtagccc ggaatgtccg caccagtcaa
     3841 gattgcggtg gatgccatgg gttccgacca cggccaggag gcggtggtgg ccggagcggt
     3901 tttggccttg cgcgaaaaaa ccaacggcag ccggcgttcg cagcctgtac ctaccgaact
     3961 catcctagtc ggtgaggaga agcaaattcg gcgcgaactg gggcgcctga aggcggagga
     4021 cctccccatt cagattgccc atgcgtccga acaggtccag atggaggagc aagccaccga
     4081 aggctggaag aaaaaagatt cttccatcgc ggtggccatg cgcctgcaga aagagcgtca
     4141 agtccaggca gtggtctccg gcggcaatac cggcgcggtg atgacgaccg cacttttgac
     4201 gctggggcgg ctgcaagggg tgatccggcc ggcgatagca tcgctttttc cgaccctccg
     4261 gcacccgacg gtgattttag acgtgggagc caatgccgac tgcaaggcgc agcacctgca
     4321 gcagttcggc gtcatgggga gcatctacta cagccacctg ctcaaacggg ccgagccgcg
     4381 ggtggggc
//
LOCUS       DBNGZA010000291         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1433117, whole genome shotgun sequence.
ACCESSION   DBNGZA010000291 DBNGZA010000000
VERSION     DBNGZA010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1433117"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..564)
                     /locus_tag="AC16FU_06280"
     CDS             complement(25..564)
                     /locus_tag="AC16FU_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2994013.1"
                     /translation="MRKGKLIISICLLAVFCAGAVQAGDLGIGVKVVTGLHSIQVTNL
                     TLTIPFVGTVAMSPLPPAKGPNDSSTVTYSLAQYGYNPTGASVSFLMDGVPHSGSYSP
                     LRYDTLYTLPFHGLPDSKISFHLCSLRGDVNNDFYTDISDIVASIQYVVFGTALPDLT
                     AGDVNGDGVVDITYIVGLV"
     gene            1011..1292
                     /locus_tag="AC16FU_06285"
     CDS             1011..1292
                     /locus_tag="AC16FU_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994014.1"
                     /translation="MSLSNRDTPTLSPILHRTVQVKAIVVGSRVTPLWMRYNGRVFPF
                     QQVLRSWREENGLTFPRWMFHLTDGLNFFELSWDTTDFCWRLWRMESQE"
     gene            1498..1746
                     /locus_tag="AC16FU_06290"
     CDS             1498..1746
                     /locus_tag="AC16FU_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994015.1"
                     /translation="MPGSCCVKLSASESRIDPRPVEIEEDEKDEIDDSLLLRHLHPEV
                     ANRPETLPTCPECNEELVAAPGLFFCLACGKNYCVENP"
     gene            1965..>2193
                     /locus_tag="AC16FU_06295"
     CDS             1965..>2193
                     /locus_tag="AC16FU_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2994016.1"
                     /translation="MGLPSALVESWNRHQGQTLLPLQVSAVDSGILTGQSLLIAAPTS
                     SGKTFCGELALAAAVARRKKAVFLVPLKAIAE"
BASE COUNT          483 a          654 c          620 g          436 t
ORIGIN      
        1 caccggcccg ccgaaaacga cgtactacac cagccccacg atgtaggtaa tgtccacgac
       61 gccgtcgccg ttgacgtcgc ccgccgtcag gtcgggcaac gcggtgccga ataccacgta
      121 ctgtatggac gccacgatgt cggagatgtc ggtgtagaag tcgttgttca cgtccccgcg
      181 cagactgcag aggtggaaac tgattttgga atccgggagg ccgtggaacg ggagcgtgta
      241 gagcgtatcg tagcggagcg ggctgtacga cccgctgtgc ggcacgccgt ccatcaggaa
      301 cgagacactg gcgccggtcg gattgtaccc gtactgcgcc agcgaatagg tgactgtcga
      361 cgaatcgttc ggccctttgg ccgggggcag aggcgacatt gcgactgtac cgacgaacgg
      421 tatggtaagc gtcaaattcg tgacctggat ggagtgcaac ccggttacaa cttttacccc
      481 aatacccagg tcacccgcct gaaccgcacc ggcgcaaaac accgccaaaa ggcagattga
      541 aataatcagt ttccccttgc gcataattac ctcctgaggc aaccgctccc ttccccgcaa
      601 aagtccgtca cttgttaatc ctcccgcacg ttcgggttcc gtaggccgaa cttctctatt
      661 gactcttaac cacttacgac cccgctaagt tccctgaatt tttttgacga aaccttaaaa
      721 aaacacttga caactgaaca tttgaacagt ataatggctg cgtgcttgga aaaggcatca
      781 aaatgacccg cggtattgaa gccccggtct tcggctgggg gaacccctgg caggaggcat
      841 tgcccaaagg cgtgtcgaag tgtcggagtg tcggagtatc ggggttaact tcccttcgac
      901 aagctcccat cgggtctgaa gaccctcagg gtcgaagaca gggtggtgag tccgccaagg
      961 cggatcgaac cacgacacct cgacaccccc cctcgacttc gctcggggcg gtgagcttgt
     1021 cgaaccgcga caccccgaca ctatctccca ttcttcatcg caccgtgcag gtgaaggcca
     1081 tcgtcgtcgg gagcagggtg acgcccctct ggatgcgtta caacggcagg gtcttcccgt
     1141 tccaacaggt gctacgctcg tggcgggaag agaacgggtt gaccttcccc cgctggatgt
     1201 tccacctgac cgacgggctg aatttcttcg aactttcctg ggacaccacc gacttttgct
     1261 ggcggctgtg gcgcatggag tcgcaggaat gagaaagacg aaacacagta gacagaacac
     1321 ggaacacagg aaaaccctgg tgccggttat tgtttcgcgt aggggcgggg tttcccccgc
     1381 ccgtgtttgc gtaggggcga cccgtacagg ccgcccggaa aaattccatc attccatagg
     1441 tcaggtcggt acgacccgta tgggcatcct gcctgacgct aaaacgagga ggaaaaaatg
     1501 cccggctctt gctgcgtaaa attgagcgca agcgaaagtc gtatcgaccc gcgtcccgtt
     1561 gaaatcgaag aggacgaaaa ggacgagatt gatgactcgc ttctcctgcg gcatctgcat
     1621 ccggaagtgg caaacagacc cgagacgctc cccacctgcc cggagtgtaa cgaagaactc
     1681 gtcgccgccc cgggcctctt tttctgcctg gcgtgtggaa aaaactactg cgttgagaac
     1741 ccgtaagagg caagttagtg tcgaagtatc gaagtgtcgg ggttgtcgag gttgcagcga
     1801 acgccagagg cgaccgaggc ggtcgcccta cgcggcagaa cttcctttcg cgtgggcgcg
     1861 gcgccctcgc cgcgctgtca cgaatcaaag aacgaattcc ttgaccccga aacctcgaca
     1921 ccccgacacc aacttttctc atgcgtattg aacaactcag aggaatgggc cttccatccg
     1981 ccctggtgga atcctggaac cggcatcagg gtcaaaccct cttgcccctc caggtctccg
     2041 ccgttgattc cggcatcctc accggccaga gtctcttgat cgccgctccg acttcttcgg
     2101 gcaaaacttt ttgcggtgaa ttggccctgg ctgcagcggt ggcccgccgg aagaaagcgg
     2161 tctttctggt tcccttgaaa gccatcgccg aag
//
LOCUS       DBNGZA010000292         3370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1964834, whole genome shotgun sequence.
ACCESSION   DBNGZA010000292 DBNGZA010000000
VERSION     DBNGZA010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1964834"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /gene="uvrA"
                     /locus_tag="AC16FU_06300"
     CDS             <1..1355
                     /gene="uvrA"
                     /locus_tag="AC16FU_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR2994017.1"
                     /translation="RIHLATQIGSRLMGVLYILDEPSIGLHQRDNKRLLNTLIGLRDL
                     GNTVVVVEHDRETIQTADFVVDLGPGAGKNGGEVVVSGPPEVVARTPRSITGQYLSGK
                     RFIPIPQRRRNGSGKYVKLIGATGNNLKNVTVQIPLGLFVCVTGVSGSGKSTLINETL
                     YRILAQDFFLAKTPPLSYQKIEGLQKIDKVIYIDQSPIGRTPRSNPATYTGVFTFIRD
                     LFAAHPEAKMKGYAPGRFSFNVRGGRCEACEGDGIIKIEMHFLPDVYVPCEVCKGKRY
                     NRETLEVKYKGKSIADVLDMTVDETLQFFENIPRIRKKLKTLADVGLGYIHLGQQATT
                     LSGGEAQRVKLSTELSKTATGNTLYILDEPTTGLHFEDIKMLLNVLNELVDRGNTVLV
                     IEHNLEVIKTADYIIDLGPEGGDDGGRIIAQGTPEQVAQAKESYTGQFLREILGNGKK
                     "
     gene            complement(1416..1691)
                     /locus_tag="AC16FU_06305"
     CDS             complement(1416..1691)
                     /locus_tag="AC16FU_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR2994018.1"
                     /translation="MTGAPPYRVEVPHQVEKTLRRLRASTPRIYERVTAILQDLATDP
                     FSGKGLKGAFIQYRSYRVGDYRILYRIETKAKVIYIVDIGHRREIYR"
     gene            complement(1688..1975)
                     /locus_tag="AC16FU_06310"
     CDS             complement(1688..1975)
                     /locus_tag="AC16FU_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR2994019.1"
                     /translation="MVTTVTLKELRPKLPEVIERVDGRMDRYIVTRRGKPVAVMMNVD
                     EYESWMETLNLMGDKKTLVRLRRAEQEIGRGNVRPLEEVLQRIETRQTKKR"
     gene            complement(2067..2813)
                     /locus_tag="AC16FU_06315"
     CDS             complement(2067..2813)
                     /locus_tag="AC16FU_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2994020.1"
                     /translation="MKFRFALSAGALAALLLLPSPGEAFPLEKFHAANALYDQGNFSG
                     AIAGYEGLLSSGTQDYRAYYNLANAYYRDKKIGYAFLNLLRAQRLAPRDDDIRTNLAF
                     LKQQALDKFPQLPDPVGSLWRPLALNELAWLFSLFYFLTIGSFAAWRFRVYPVRVFRW
                     GAVTFGLVALIFLGLLYGKYQSDYAQDHGVVIAQQVEVKSGPGEDFLTQFTGHEGLTL
                     TVLQMRPDWYEALFPNGVKGWVKKETVELL"
     gene            complement(2810..>3370)
                     /locus_tag="AC16FU_06320"
     CDS             complement(2810..>3370)
                     /locus_tag="AC16FU_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994021.1"
                     /translation="IASLPASGEGTPVVASDLRYLKTNADHLSGSAQPVIFRLWFWGL
                     QLVPLLGLGGLVAYQKHQWKLATDVGYARSRRAHRMAKKRLATALDLIGINGQKEFFA
                     EVSKAVLLYVADKFNTSTYGMTTPEMEDFLKTKGVSPELVKRLTELLTSCDLAKFAPT
                     YVTQTDMKKKLDEAEEMIVSLEGVRR"
BASE COUNT          759 a          941 c          949 g          721 t
ORIGIN      
        1 aaagaattca cctggccacc cagatcggct cccgcttgat gggcgtcctt tacattctgg
       61 atgaaccctc catcggcctg caccagcggg ataataaacg ccttttgaac acgctcatcg
      121 gcctgcgcga tttaggcaac accgtcgtcg tcgtggagca cgaccgggag acgattcaga
      181 ctgccgattt tgtggttgat ttaggtcccg gagcgggcaa gaacggcggc gaagtggttg
      241 tctctggccc gccggaggtg gtcgcccgta ccccgcgctc catcaccggc cagtatttat
      301 ccggcaagcg tttcattcca attccccagc gaaggcgcaa cggcagcggc aagtatgtga
      361 agttaatcgg cgccaccggc aacaatttga agaacgtaac cgtccaaatt ccgctgggac
      421 ttttcgtctg cgtcaccggc gtttccggct cggggaagtc gactttgatc aatgagacgt
      481 tgtaccgaat cctggctcag gatttttttc tagcgaaaac gccgccactc tcttatcaaa
      541 aaattgaggg gttgcagaaa attgacaagg tgatctacat cgaccagtcg cccattggcc
      601 ggacgccccg ctccaatcca gccacctaca ccggtgtttt cactttcatc cgcgaccttt
      661 ttgcagcaca tccagaggcg aagatgaagg gctacgcccc ggggcggttt tctttcaatg
      721 tccgcggcgg ccggtgtgag gcttgcgagg gcgacggcat catcaaaatt gagatgcact
      781 ttttgcctga cgtttacgtt ccctgcgaag tctgtaaggg gaagcgctac aaccgcgaga
      841 ctttggaggt gaagtataaa gggaaatcga ttgccgacgt gctcgatatg actgtggacg
      901 aaaccctgca attctttgag aatatcccgc gcatccgcaa aaaactgaag acgctggccg
      961 acgtgggtct gggctatatc cacttgggcc agcaggccac gaccctttcc ggcggcgagg
     1021 cccagcgggt gaaactttcg accgaactct ccaaaaccgc caccggcaat acgctctaca
     1081 ttttggacga gcccacgacc ggcctgcact tcgaggacat caagatgctt ctgaacgtcc
     1141 tgaacgaatt agttgaccgg ggcaatacgg tcctggtgat cgagcacaac ctggaggtga
     1201 ttaagaccgc cgactacatc atcgacttag gcccggaagg gggcgacgac ggggggagaa
     1261 tcatcgctca gggcacgccg gagcaggtgg cgcaggccaa agagtcgtac accgggcagt
     1321 ttttaaggga gatactgggg aatggaaaga aatgatgaac cgcaggatga attcatagca
     1381 gatggagcag aagaatcaga tcgaaagaaa actatttacc ggtatatctc tcgccggtgg
     1441 ccgatgtcca cgatgtagat aactttggcc ttcgtctcga tgcggtagag aatacggtaa
     1501 tctccaaccc ggtaagaacg atactggata aacgccccct taagcccttt acctgaaaac
     1561 gggtcagtgg ccagatcttg aagaatcgct gtaacccttt cgtaaattcg tggcgtggaa
     1621 gctcgcaggc ggcgcagggt tttctcaacc tgatgcggaa cctcaacgcg gtatggcggc
     1681 gcgccggtca ccgcttctta gtctgccgtg tttcaatgcg ctgtaaaact tcttcgagcg
     1741 gacgtacgtt gccccgtccg atttcctgtt cggcccgcct caggcggact aaagttttct
     1801 tatcccccat aaggttgagc gtctccatcc aggactcgta ctcgtccaca ttcatcatca
     1861 cggcgaccgg cttcccgcgc cgggtgacga tgtagcggtc catccggccg tcgacccgtt
     1921 cgatgacttc aggcaatttg gggcggagtt ccttcagagt aacggttgtc accatagtaa
     1981 gtaagtatac gcaattgtgt aaactttaat agtacccttt tcagttcggc gacttcgtct
     2041 ggagaccgat gcttgtcagt gcggacttag agcaactcca ccgtctcttt cttcacccac
     2101 ccttttaccc cgttggggaa gagcgcttcg taccagtcgg ggcgcatttg taggaccgtg
     2161 agggtcaatc cctcatggcc ggtgaactgg gttaagaagt cctcgcccgg accgcttttc
     2221 acctccacct gctgggcaat cacgacaccg tggtcctgcg cgtaatcgct ctggtatttc
     2281 ccgtagagca gaccgagaaa aattaaagca accagtccaa acgtgaccgc accccagcgg
     2341 aacacccgca ccggatagac ccgaaaccgc cacgcggcaa aagaacctat cgtcaggaag
     2401 taaaagagcg aaaaaagcca ggccagttca ttcagggcca agggccgcca gagcgagcca
     2461 acgggatcgg gcagttgggg gaatttgtcc aaggcctgct gtttcaaaaa agcaagattg
     2521 gtccggatgt catcatcccg cggggccagg cgctgggcgc gcaggaggtt cagaaaggca
     2581 tagccgattt tcttgtcccg gtagtaggca ttggccaagt tgtagtaggc ccggtaatcc
     2641 tgcgttcccg aagaaagcag accctcgtat ccggcgatgg caccgctgaa attcccctgg
     2701 tcgtagagcg cattggcggc gtggaatttc tccagtggaa acgcttctcc cggtgagggg
     2761 agcaaaagca gggccgcaag cgcgccggcc gagagggcaa aacggaattt caccggcgca
     2821 cgccctccag cgacacgatc atctcttcgg cctcgtctaa cttctttttc atatcggtct
     2881 gggtcacgta ggtcggcgca aatttggcca ggtcgcaact ggtcaaaagt tcggtgagtc
     2941 ttttcaccag ttcgggtgat acccctttgg ttttgaggaa atcttccatt tccggcgtgg
     3001 tcatcccgta cgtggaggta ttgaatttat ctgcgacgta gagcaatact gccttcgaca
     3061 cttcggcaaa aaattctttc tggccgttga tgccgatcag atcaagcgcg gtggccaagc
     3121 gttttttggc catccggtgc gcacggcggg agcgggcgta gcccacgtcg gtcgccagtt
     3181 tccactggtg tttctgatag gccaccagcc cgcccagacc cagaagcggt accagttgca
     3241 ggccccagaa ccagagccgg aagatcaccg gctgggccga accggaaagg tggtcggcat
     3301 tcgttttgag gtagcggagg tcggacgcca ccaccggcgt gccctccccg gaagcgggca
     3361 gtgaggcaat
//
LOCUS       DBNGZA010000293         3299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1536741, whole genome shotgun sequence.
ACCESSION   DBNGZA010000293 DBNGZA010000000
VERSION     DBNGZA010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1536741"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16FU_06325"
     CDS             complement(<1..851)
                     /locus_tag="AC16FU_06325"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2994022.1"
                     /translation="MSGRAGEKEIRAVKRAKGGRPAARRIVILGAGYGGIACVTRLAR
                     LTQRHPEVEIHLCDRNTYHLLETRLHEAAMRKAEVAIPIARIIRRWNVHFHLGEVSQI
                     DLSVRQVVLGEKTLAYDYLVIALGSKTNFYAIPGLAELALQLKTLEDSLKIRQRVDVM
                     FARARSEKDPEKRRRLLTFVIGGGGLTGAELAGEMTERVEKHCHDYEIPRGEVQIILL
                     EAGPRIVPSLSEKQSALSVERLQSRGVRILTNTKVVGMEPGKVLIEPGEPIPAETLVW
                     TGGIRASR"
     gene            complement(802..2949)
                     /locus_tag="AC16FU_06330"
     CDS             complement(802..2949)
                     /locus_tag="AC16FU_06330"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR2994023.1"
                     /translation="MNLAEFIIKVESFNANVNIPLIRKAYEFSDRAHRGQKRESGDPF
                     IEHCLEVAFILAELHLDSATIAAGLIHDVVEDTEVTIDRVRREFGEEIGLLVDGVTKL
                     SGLKFTSAEEEQVENFRKLLLSMAKDIRVIIIKLADRLHNMRTLEHLPREKISRIAEE
                     THDVYAPLAHRFGMARIRWELEDLAFKYLNAEAYNDIARKINEKRKEREEYIQAVVRP
                     LTDALQRAGIRAEITGRAKHFDSIYRKMLKRDKPFEEIYDLFAIRIVVHTVEECYHVL
                     GVIHSLWTPVTDRFHDYIALPKSNMYQSLHTTVVGPSGKMVEIQIRTEAMHRTAEFGI
                     AAHWLYKEGRHQADAADRQMAWLREVLEWQKELTDPAEFLEYLKIDLFHDDIFVFTPR
                     GEIKQLPRGATPLDFAFAVHTDLGFRCTGARINGRIAPLATPLASGDEVEILASPHRV
                     PHEDWLTIVKTTRARNKIRHWLKQKGYERQVQLGRELLEKECRKKRLAFPSESELNDL
                     ALGMSFSSVEGLYAAMGEGDVSPLVILHKLQPKEEKPSLLARVAERIRGAPGGIRVQG
                     MSDVLFRFAQCCQPVPGEPIVGFITRGRGVSIHRSDCTNVLQNNHGPERRIEVAWDVA
                     KEQRFLVRLSVIVEDRKNMLHDITDAITETDANVRAAEIKGGEMGPAGSFVVEVKNAS
                     HLKDVVSHIRKVKGVVAIERARGGEGDSSGQAS"
     gene            complement(3040..>3299)
                     /locus_tag="AC16FU_06335"
     CDS             complement(3040..>3299)
                     /locus_tag="AC16FU_06335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2994024.1"
                     /translation="ITNSTGPMHIAAAVGTPVIAFFCPIRTASPRRWGPRGVGHRVFL
                     PPITECTCAVNRCERGNCMAQITVEQAFTSAVSVLASRSRR"
BASE COUNT          620 a         1003 c          964 g          712 t
ORIGIN      
        1 cggctggccc gtatgccccc cgtccagacc aaagtctcgg ctgggattgg ctcgcctggt
       61 tcaatgagga ctttgcccgg ctccataccc actactttcg tattcgtcag aatacggacg
      121 cctctggact ggagccgttc gacgctcaaa gcgctctgtt tttccgaaag cgaaggcacg
      181 atgcgcggcc cggcttccag gagaattatt tgaacttcac cccgcggaat ttcgtagtcg
      241 tggcagtgct tttccacccg ctcggtcatc tccccggcta attcggcgcc ggtcaatccg
      301 ccgccgccaa tgacaaaggt caaaagccgg cggcgtttct ctgggtcttt ctctgaccgg
      361 gcccgggcga acatgacgtc cacccgctgg cggattttaa gggaatcctc caaggtcttc
      421 agttgcagcg caagttcggc cagaccggga atggcgtaga aattcgtctt gctccccaaa
      481 gcaatcacca ggtaatcgta ggccaaagtt ttttcaccca gtacgacctg tcggacagag
      541 agatcaatct gactcacttc gcctaaatgg aagtggacgt tccagcgccg gatgatacgg
      601 gcgatcggga tcgccacttc ggctttgcgc atcgcggcct catgcaggcg ggtttctaaa
      661 agatggtagg tattccggtc gcaaaggtgg atttccacct cggggtgacg ttgggtcaaa
      721 cgggctaaac gggtgacgca ggcaatgccc ccgtaaccgg cgccgaggat gacgatgcgc
      781 ctggccgctg gcctcccccc tttagctcgc ttgaccgctc gaatctcctt ctcccccgcg
      841 cgcccgctca atggcgacga cgcctttcac cttcctaata tgggagacga cgtccttcag
      901 atgggatgcg ttcttgacct caaccacaaa actccccgcc gggcccattt cgcccccctt
      961 gatttcggcg gcgcgcacgt tggcgtcggt ttcggtgatg gcgtcggtaa tgtcgtgcag
     1021 catattcttg cggtcttcga cgatgaccga caaccggact aagaaccgct gctccttggc
     1081 cacgtcccag gcaacttcga tccgccgctc cggcccgtgg ttgttctgga ggacgttcgt
     1141 gcagtcgctc cggtgaatcg aaacgccccg accgcgggta atgaaaccga cgatcggctc
     1201 gcccggcacc ggctggcagc actgggcaaa ccggaagagc acgtcgctca ttccctgcac
     1261 ccggatgccg cccggggctc cccgaatccg ttcggccacc cgggccagaa gggacggctt
     1321 ctcctctttc ggctggagtt tgtggaggat gaccagcggg gaaacatcgc cctcacccat
     1381 cgccgcatag agaccttcaa cggaggagaa cgacatcccc agcgccaggt cgttcaactc
     1441 gctctctgat gggaaagcca ggcgcttctt gcggcattct ttttccagaa gttcccggcc
     1501 cagttgcacc tgccgttcat accccttttg cttcaaccag tgccgaattt tgttccgggc
     1561 ccgcgtagtt ttgacgatgg tcagccagtc ttcgtgagga acccgatggg gagacgcaag
     1621 gatttccact tcgtcgccgg aggcaagcgg cgtggccagg ggagctatgc ggccgttgat
     1681 ccgcgcgccg gtgcagcgga agcccagatc ggtgtggacg gcgaaggcaa aatctaatgg
     1741 tgtggccccg cgcggcagct gcttaatttc tccccgcggc gtgaagacaa agatgtcgtc
     1801 atggaagagg tcgatcttga gatactccag gaactcagcc gggtcggtca gttccttctg
     1861 ccattccagc acctcccgca gccaggccat ctggcggtcg gctgcatcgg cctggtgccg
     1921 cccttctttg tagagccagt gcgcggctat gccgaactcg gcggtgcggt gcatcgcctc
     1981 ggtgcggatt tgaatttcca ccatttttcc ggagggaccc actaccgtgg tgtggagcga
     2041 ctggtacatg ttgctcttcg gcagagcgat gtagtcgtgg aagcggtcgg tcaccggggt
     2101 ccacagcgag tgaatcactc ctaaaacgtg gtagcactcc tccacggtat gaacgacgat
     2161 ccggatagcg aataagtcgt agatttcttc aaacggcttg tcccgcttga gcattttgcg
     2221 atagatggaa tcgaagtgtt tggcccgccc ggtgatttcg gcccggatac cggctcgctg
     2281 cagagcatcg gtgagcggac gcaccaccgc ctggatgtac tcctcccgct ccttcctttt
     2341 ctcgttgatc ttacgagcga tgtcgttgta ggcctccgca ttcaggtatt tgaaggccag
     2401 atcttccaac tcccagcgga tgcgcgccat tccaaagcgg tgggctaagg gtgcgtaaac
     2461 gtcgtgggtc tcctcggcga tccggctgat tttctccctg gggaggtgct ctaatgtccg
     2521 catgttgtgc aggcggtcgg ccagtttgat gatgatcacc cggatgtcct tggccatcga
     2581 gaggagcagt ttgcggaaat tttccacctg ttcttcttcg gcgctcgtga acttgagccc
     2641 gctcaatttg gtcaccccgt ccaccagcaa gccgatttcc tcgccgaact cccgccgtac
     2701 ccggtcaatc gtcacctcgg tgtcttccac cacgtcgtgg atcaaaccgg cggcgatcgt
     2761 ggccgagtcg aggtgcaatt cggccaggat gaaggccacc tccaggcagt gctcgatgaa
     2821 tggatcgccc gactcccgct tttgcccccg gtgtgcccgg tcggaaaact cgtacgcttt
     2881 ccggatgagc ggaatgttga cgttggcgtt gaacgactcg actttgatga tgaattcagc
     2941 caggttcata atcagcgttc cggcgtcttg cgaccgcgca cctcacatac aatatacgcc
     3001 ctctcaccgg cggaggtaag cgcacttccc tcttaaacgt tatcgacggg aacgggaagc
     3061 gagaacagaa accgcgctgg tgaaagcctg ttcgacagtg atctgcgcca tgcagttgcc
     3121 gcgctcgcaa cggttcaccg cacaggtaca ttcggtgatg ggagggagaa aaacacggtg
     3181 gcccactccc cgcgggcccc agcgtcgtgg ggaggccgtg cgaatcgggc aaaaaaaggc
     3241 aatgacgggt gtgcccacag cggcggcgat gtgcatgggg ccggtgctat tggtgatga
//
LOCUS       DBNGZA010000294         3764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1113876, whole genome shotgun sequence.
ACCESSION   DBNGZA010000294 DBNGZA010000000
VERSION     DBNGZA010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1113876"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="cysS"
                     /locus_tag="AC16FU_06340"
     CDS             <1..1277
                     /gene="cysS"
                     /locus_tag="AC16FU_06340"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR2994025.1"
                     /translation="FRTNVFQDLLRRYLEYKGFRVTQVMNLTDIDDRIIAGVRSKKLP
                     LREYTEPYKQAFFADLDALGIKRADFYPEATVHVPEMAALVERLLEKGHAYRQDGSIY
                     FKIGSFPTYGLLSRMKLDELKAGARVSADSYEKEQVSDFALWKAWDEQDGDIFWETPL
                     GKGRPGWHLECSVMSMKYLGEHFDIHTGGVDLIFPHHENEIAQSEAATGKKFVNFWLH
                     AEHLIVEGRKMSKSLGNFYTLRDILAKGFSPKAVRYLLLSVHYRQQLNFTFDGLEAVR
                     NAVERLLDFKRNVTDARSAGDNLKVAELVAGTKQGLEANLDDDLNISGALGVLFDFVR
                     DMNKIIADRGLSAKDKTQVLEFLGIVDGVLGLLKTPDEELDSAVKGLIAQRDAARAQK
                     DFTAADAIRSRLDQLGIILEDTVQGTKWKKRL"
     gene            1352..2530
                     /locus_tag="AC16FU_06345"
     CDS             1352..2530
                     /locus_tag="AC16FU_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994026.1"
                     /translation="MRRRYFFWFLIFSLILVQGSWSQLPPKKLVVHKNLSGQSELFWF
                     FPDAERKELFYDDGVPQIGFYVTSAWLNNRAGVRFTPQHFPYFVDSIRIFISHDNPPS
                     PGDSASPFQVALYSDSGGLPGSPITDLVLTSADSTAWQGNGQWLPVAFGYCPPESVSF
                     WGVFHWFQATPAAPKIGADDHHDIFVSATWPSGTSQQEWDIFSQFNFMFRAVTFSNGP
                     TTADSFSVHRAADSGFVPTNTSRIATLSAPIDHFVGQPAGPESVWYYRVTAWENDQES
                     PGSNAVRIVQSPTAVEGESDFPIPQQVTLLPNYPNPFNARTVIRYALDKEAFVSLDIF
                     DLLGRRVKTLFTASQKAGLHQVAWEGTDDRGRTVPSGIYFVRLTAGAQVQRGRMLLLK
                     "
     gene            2542..2745
                     /locus_tag="AC16FU_06350"
     CDS             2542..2745
                     /locus_tag="AC16FU_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HIR2994027.1"
                     /translation="MKEALVILAAIICVILLVQNSTLVNVKFLLWHKFLSLSVVVLTA
                     FILGAVFGYGISSRKKKQTQVKK"
     gene            2750..3175
                     /locus_tag="AC16FU_06355"
     CDS             2750..3175
                     /locus_tag="AC16FU_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018032575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR2994028.1"
                     /translation="MGYRFLDKVAIADTAFEAEAPTLPELFATCAEATFAVMVDLSTV
                     TPSEERRVELTSPSLDRLLFDWLAELIYLKDRDYFVFSRFEVKIEQNEQWRLQAVAGG
                     EKIKPEKQRLLTDVKAVTYHMFEVKKQDGGWLARVVLDT"
     gene            3283..>3764
                     /locus_tag="AC16FU_06360"
     CDS             3283..>3764
                     /locus_tag="AC16FU_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR2994029.1"
                     /translation="MWEIAPDQKPGMRVPAWILGTDALVNAMDQGVYNQVTNVACLPG
                     IQKVSICMPDGHWGYGFPIGGVAAFDLREGVISPGGIGFDINCGMRLVRTNLTYDEVK
                     PKLEQLMHTLFRLVPSGVGSKSSLRLSRQEFREVMVHGAQRIIKMGYGWPGDVERIEE
                     "
BASE COUNT          815 a         1008 c         1103 g          838 t
ORIGIN      
        1 atttccgcac caacgtcttc caggacctcc tgcggcgcta cttggagtac aaaggcttcc
       61 gcgtcacgca ggtgatgaat ttaaccgaca tcgacgaccg gatcatcgcg ggggtgcggt
      121 caaaaaaact gccgctccgt gagtacacgg agccttacaa acaggccttt ttcgccgact
      181 tagacgcgct gggcatcaag cgggctgact tttacccgga ggcgaccgtg cacgtgccgg
      241 agatggcggc tttagtcgaa cgacttttgg agaaagggca cgcctaccgg caggatgggt
      301 caatttactt caaaatcggc tcgttcccga cctacgggct gctctcgcgc atgaaacttg
      361 acgaactgaa agccggggcg cgcgtttccg ccgattcata cgaaaaagaa caggtctccg
      421 actttgccct ctggaaagcc tgggatgaac aggacggcga cattttctgg gaaacccctc
      481 tgggcaaggg aaggcccggg tggcatttag agtgctcagt tatgagcatg aaatatctgg
      541 gcgagcactt cgacattcac accggcgggg ttgatttgat tttcccccac cacgagaacg
      601 aaatcgcgca gagcgaggcg gcgaccggga agaagttcgt caacttttgg ttgcacgccg
      661 agcacctgat cgttgaaggc cggaagatgt cgaagtccct gggcaacttc tacaccctgc
      721 gcgacattct ggccaagggg ttttccccca aagcggtgcg gtatcttttg ctctcggttc
      781 actaccgcca gcagctgaat ttcactttcg acggcctgga agcggtgcgc aatgcagtcg
      841 aacgtctctt agattttaag cgcaatgtca ctgacgcccg gagtgcagga gacaatctga
      901 aggttgccga gctcgtcgct gggacgaagc agggcttgga ggcgaatctt gatgacgatt
      961 tgaacatttc cggggcactg ggggtgctgt tcgatttcgt gcgggacatg aataaaataa
     1021 tcgccgaccg aggtctttcc gcgaaagaca aaacgcaggt gctggaattt ttggggatag
     1081 ttgacggcgt gcttggactc cttaagacgc cggacgagga actcgattcc gcggtcaagg
     1141 gactcattgc ccagcgggac gcagcccgcg cgcagaaaga cttcacagcc gcggatgcca
     1201 tccgtagccg gctggaccag ttaggcatca ttctggaaga cacggtgcag ggcaccaagt
     1261 ggaagaagcg gttgtagggc cggcttgact tttcttttcc aaaacgttta atcacctaca
     1321 aactcgatag ttccggtcgg aggaggcgtc aatgcgtcgc cggtactttt tttggtttct
     1381 catcttctca ttgattctgg ttcagggttc ctggtcacaa cttcccccca aaaaacttgt
     1441 ggtgcacaag aatctttccg gccagtcgga actgttctgg tttttcccgg atgcagagcg
     1501 gaaggagtta ttctacgatg acggcgtgcc ccagatcggt ttctacgtca cctcggcctg
     1561 gctgaacaac cgggcgggag tgcgctttac gccgcagcat tttccttact ttgtagattc
     1621 gattcgcatt ttcatcagtc acgacaatcc gccatccccg ggggattctg cctcgccttt
     1681 tcaggtagca ctctactccg attcaggggg attacccggg tcgccgatca ccgatctggt
     1741 gctcacaagt gcggacagta ccgcttggca ggggaacggt cagtggcttc cggtcgcttt
     1801 cgggtactgc ccacccgaat cggtttcctt ctggggggtg ttccactggt ttcaggccac
     1861 gcccgcggcg cccaaaattg gggccgacga ccaccacgac atcttcgttt cggcgacgtg
     1921 gccatcgggc acttctcagc aggaatggga cattttctct cagtttaact ttatgtttcg
     1981 tgccgtcaca ttttccaatg gcccaacaac agccgatagt ttttcggttc atcgggcggc
     2041 agattccggt tttgttccaa cgaacaccag ccggatcgca accctgtctg ctccgataga
     2101 tcacttcgtg ggtcaacccg ccgggccgga atcggtctgg tactaccggg tgacggcgtg
     2161 ggagaatgac caggagagtc cgggttcgaa cgcggtgcgc atcgtccaat ccccgacggc
     2221 cgtagaaggt gagtcagatt ttcccatccc gcaacaagtt acgctcctcc ccaactaccc
     2281 caacccgttc aatgcccgca cggtgatccg gtatgcgctg gataaagaag cgttcgtctc
     2341 gctggatatt tttgaccttt tgggccgcag agtgaaaacg cttttcaccg ccagccagaa
     2401 ggcagggttg caccaggtcg cctgggaagg aactgatgac cgggggcgaa ccgtaccttc
     2461 gggaatctac ttcgtccggt taaccgccgg ggcgcaggtg cagaggggca gaatgctgct
     2521 cctgaaatag tcaacccgac aatgaaagaa gcccttgtca tcctggccgc catcatctgc
     2581 gtgattcttt tggttcaaaa cagcacgctg gtaaacgtca agtttctcct ctggcataaa
     2641 tttctctcgc tttccgtagt ggtgttgacg gctttcattt tgggcgccgt cttcggctac
     2701 ggaatttcgt cacgtaaaaa gaagcaaaca caagtcaaga agtagcggta tgggctaccg
     2761 cttcttagat aaggtcgcaa tcgccgacac ggcttttgag gcggaggccc ccacgctgcc
     2821 ggagcttttt gccacctgcg cggaggcgac ctttgcggtg atggtcgatt tgagcaccgt
     2881 gacgccgtct gaggaacgcc gggtcgaatt gacctcgccc tccttagatc ggctgctctt
     2941 cgactggctg gcggagttga tctacctcaa agatcgcgat tacttcgtct tttcacgttt
     3001 cgaagtcaaa attgagcaga atgagcagtg gcgtttacaa gccgtcgcag ggggggaaaa
     3061 gatcaaaccc gaaaagcagc ggctgttgac cgatgtcaaa gcagtgacgt accatatgtt
     3121 tgaggtcaaa aagcaggacg ggggctggct ggcgcgggtg gtgctggaca cctgaaaaga
     3181 caggatttcg gattcgggag ttatgagttc ggggaaagag caaacccaag gacaattcat
     3241 agaggtgatg acgtagtgga aaagaaacaa atcgcgccgg ggatgtggga aattgcgccg
     3301 gatcaaaaac cggggatgcg ggtgccggcc tggattctgg ggacggacgc attggtcaac
     3361 gcgatggatc agggggtcta caaccaggtg accaacgtcg cctgtttgcc cggcatccag
     3421 aaagtttcga tctgcatgcc ggacggccac tggggatacg gctttcccat cggcggggtg
     3481 gcggcgttcg atttgcgcga gggcgtcatt tctcccggcg ggatcgggtt tgacatcaac
     3541 tgcgggatgc gcttagtgcg gacaaatctc acctacgacg aagtcaaacc aaaattggaa
     3601 caactgatgc acacgctctt ccggctcgtg ccttccggcg tcggctccaa gagttctctg
     3661 cgcctctcca ggcaggagtt ccgtgaagtg atggtgcacg gcgcgcagag aatcatcaag
     3721 atgggctacg gctggccggg ggatgtcgaa cgcatcgaag agca
//
LOCUS       DBNGZA010000295         2564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2704420, whole genome shotgun sequence.
ACCESSION   DBNGZA010000295 DBNGZA010000000
VERSION     DBNGZA010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2704420"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC16FU_06365"
     CDS             complement(<1..738)
                     /locus_tag="AC16FU_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR2994030.1"
                     /translation="MGATLAEKVIAGKVSHPVKAGELVITPVDLALAQDGTGPLTIRQ
                     IQKLGVKKVANPNRVIFFLDHSAPASRKELANDHNFIRQFAAQTGVQVSEIGEGISHQ
                     VVAEWHVNPGEVLVGADSHTCTGGALCAFATGMGSTDVAITIALGRTWLRVPETFKIS
                     ICGDFSPGVYPKDLILHLIGLIGADGATYKALEFCGPTIEAMAMHDRLTLSNMAVEAG
                     AKVGMIASDRITHQYLKKMGRPEKWREI"
     gene            complement(803..>2564)
                     /locus_tag="AC16FU_06370"
     CDS             complement(803..>2564)
                     /locus_tag="AC16FU_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR2994031.1"
                     /translation="QKKYERSVHGVFACVTCHHDIKEIPHPEKLAKVDCSSCHTETVE
                     KYKQGVHGMALTKGNRGSPDCGGCHTAHAVLPASDPKSPTARKNIPDLCGKCHGDLKF
                     VEEQTGIISARPFFNYKESVHGKALQKGDEQAAVCSDCHKSHDIRPPNDPGSTIFKAN
                     VPQTCGRCHAAVAAEYVASIHGQALLAGISKSPVCTDCHGIHTIKARIDPKSPVAAQA
                     LARTTCIQCHESEGLSREYGIPAKRISTYLDSYHGLASRFGSTVVANCASCHGIHNIF
                     PSSDKRSLVHPDNLAKTCGKCHPGASENFARGKIHVGVAQAQDVGTRVVKWVTIIYIF
                     LIVAVVGGMVLHNVLDWSRLVWGSWRKEVPGLLVKFTAFQRAQHIVLFISFFSLALTG
                     FALKYPENFHWLFGSDENLRRVLHRIFAVIFIADSIVHLAHLLFSPAGKRLLAGLIPK
                     KRDLAQLVGNLKFYFGLAKTKAPEYLYGYVEKAEYWALVWGGIIMAATGLVLWYKSLF
                     LRFWPKWSIDVATTIHFYEAILATSAIFVWHFYAVIFKPGVYPMNWAWLTGRVSGAEI
                     EPEEAAKRETPKPSGEERAS"
BASE COUNT          502 a          746 c          745 g          571 t
ORIGIN      
        1 gatttcccgc catttctcgg gccggcccat cttcttcagg tactggtggg tgatccggtc
       61 ggaggcgatc attcccacct tcgcgcccgc ttccaccgcc atgttcgaga gcgtcaggcg
      121 gtcgtgcatc gccatcgcct cgatggtcgg gccacagaac tccagcgctt tgtaggtggc
      181 gccgtcggcg ccgatcaagc cgatcaggtg caaaatcaaa tctttcgggt agactcccgg
      241 agaaaaatca ccgcaaatgg agattttgaa tgtttccggc acccgcagcc aggtgcggcc
      301 taaggcaatg gtgatggcca cgtcggttga ccccatgccg gtggcaaaag cgcacagagc
      361 gccgccggta caggtgtggc tgtcggcccc gactaatact tcgccgggat tgacgtgcca
      421 ttccgccacc acctgatggc tgatcccttc gccgatttcg gagacctgca cccccgtttg
      481 cgccgcgaac tggcggatga agttgtggtc attggccagc tccttccgcg acgccggtgc
      541 tgagtgatcg aggaaaaaaa tcacccggtt gggattggcc acctttttga ctcccaattt
      601 ctggatctgc cggatggtga gcggcccggt gccgtcctgc gccagcgcca gatcgaccgg
      661 cgtgatgacc agttcgcccg ctttgaccgg atggctcacc ttccccgcta tgactttttc
      721 ggctaacgtc gcacccatgt tctcctctct tctatttgaa ggcatacgaa cccctggccg
      781 tcggccgcgg tgtccttccc gactaactcg cgcgttcttc cccgctcggt tttggggttt
      841 cgcgtttcgc cgcttcttcc ggttcgattt ctgctccgga aaccctcccg gtcagccagg
      901 cccagttcat cggatagact ccgggcttaa agataaccgc atagaagtgc cagacgaaaa
      961 tggcggaagt cgccaggatc gcttcgtaga aatgaatcgt cgtcgccacg tcgatcgacc
     1021 actttggcca gaagcgcagg aatagactct tgtaccagag caccaggccg gtggcggcca
     1081 tgataatgcc gccccagacc aaagcccagt attcggcttt ttccacgtag ccgtacaggt
     1141 actccggcgc tttcgttttg gccaggccaa agtagaactt cagattcccg accagctggg
     1201 ctaagtccct tttcttcgga atgaggccgg ccagcaggcg ttttcccgcc ggagaaaaca
     1261 gaaggtgcgc caggtgtacg atgctgtcgg caatgaatat gacggcgaaa atccggtgca
     1321 gcacgcgccg taagttttcg tcagagccga aaagccagtg aaaattctcc ggatatttca
     1381 gcgcgaaacc ggtcagggct aaagaaaaga agctgatgaa gaggacgatg tgctgcgccc
     1441 gctggaaggc ggtgaatttc actaagaggc cgggtacctc tttccgccaa ctgccccata
     1501 ccaatcgtga ccagtctagg acgttgtgga gtaccatccc gccaacgacg gcgacgatga
     1561 gaaagatgta aataatcgtc acccatttga ccacgcgtgt gccgacgtcc tgtgcctgcg
     1621 ccacccccac gtggattttc ccgcgggcga aattttccga cgcaccggga tggcacttcc
     1681 cgcaggtctt ggccaggtta tcgggatgga ctaacgagcg tttgtcggaa gacgggaaga
     1741 tgttgtggat gccatggcag gaggcacagt tggctaccac ggtggaaccg aagcgcgatg
     1801 ccaacccgtg atagctgtcg aggtacgtgg aaattctttt tgccggaatg ccgtactcgc
     1861 gcgacaaccc ctcactttcg tggcactgga tacaggtcgt ccgtgccagc gcctgcgccg
     1921 ccaccggtga cttcggatca attctggcct tgatggtgtg aatgccgtgg caatcggtgc
     1981 agacgggtga tttcgatata ccggccaaaa gcgcctgtcc atggatggag gccacgtact
     2041 ctgcggccac ggcagcgtgg caccgcccgc aggtttgcgg gacattggcc ttgaaaatcg
     2101 tcgaaccggg gtcattgggc ggccggatat cgtgactttt gtggcagtcc gaacagactg
     2161 ccgcttgctc atcccctttt tgcagcgcct tcccgtggac tgactctttg taattgaaaa
     2221 agggccgggc ggagatgatg ccggtttgct cctccacgaa tttcaaatcg ccgtggcatt
     2281 tcccgcacag gtcagggatg tttttccgtg cggtcggtga cttcggatcg gaggcgggca
     2341 ggaccgcatg cgcggtatgg cagccgccgc agtcgggaga gccgcgattc cctttggtta
     2401 atgccatccc gtgcactccc tgcttatact tttcaaccgt ctcggtgtga caggaggagc
     2461 aatcgacttt ggccagtttt tccgggtggg gaatttcttt tatgtcgtga tggcaggtaa
     2521 cgcacgcaaa aacgccgtgg actgagcgtt catatttttt ctgg
//
LOCUS       DBNGZA010000296         1590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2175106, whole genome shotgun sequence.
ACCESSION   DBNGZA010000296 DBNGZA010000000
VERSION     DBNGZA010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2175106"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /gene="ispF"
                     /locus_tag="AC16FU_06375"
     CDS             <1..856
                     /gene="ispF"
                     /locus_tag="AC16FU_06375"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR2994032.1"
                     /translation="VSPEKIEAICDAQNDSPACLLALPVKETLKEGGDGTVRRTIERE
                     GIFIAQTPQGFQKSLLEQALEAARKANFTVTDDAALVERLGVKPHIIPGEPTNIKITT
                     PEDLALAERLLGLPPAQSRVGFGYDSHRLVAGGRPLVLGGVIIPFEKGLAGHSDADVL
                     CHAIADALLGSLGLGDIGQHFPDTEEKWRGISSLEILRQVAVKVTAGHGEVTSVDATV
                     VAEKPRLGPYIEKMREKIAETLGLAVSAVSIKAKTAEGLGSIGAGEAMAAYAVASVNQ
                     KKVEDGRR"
     gene            916..1560
                     /locus_tag="AC16FU_06380"
     CDS             916..1560
                     /locus_tag="AC16FU_06380"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR2994033.1"
                     /translation="MEAFSARAQELLDLATSYGVWWLIFCYVFLYLSAIVESLFPPYP
                     GDVVTFAGGYLASLGKLNFPLVFFATSLGSLSGLLLLYAIGQRHGRRLFQKEQSYWRT
                     PAHLAKVERWFARYGAKVIIASRFLSGIRSGIALAAGIGNIGWRRMVLYGTVSILLWN
                     GLILFVSSFVEQNWQRLYGLLLLYNKVLFGGLLLTAIGFGIWWWRKRQVPKGAA"
BASE COUNT          345 a          399 c          499 g          347 t
ORIGIN      
        1 ggtgtcgccg gagaagatag aggcgatctg cgacgcgcag aacgattccc cagcctgtct
       61 actcgcgctg ccggtcaaag aaacgttgaa agaaggcggg gacggaacgg ttcggagaac
      121 gatagagcga gagggaattt tcatcgccca aacgccgcag ggattccaga agagtctcct
      181 tgagcaagca ctggaggcgg ccaggaaagc gaattttacg gtcactgatg acgccgcact
      241 ggtggagcgt ctcggtgtca aaccacacat cattcccgga gagccgacga atatcaaaat
      301 caccacgccg gaggatctgg cactggcgga gcgcctttta ggtctgccgc cggctcagag
      361 tcgggtgggt ttcggatacg acagccaccg cttagtagcg gggggacggc ctctcgtttt
      421 aggtggagtg atcattccct tcgagaaagg gctagccggg cattccgacg ccgacgttct
      481 ctgccacgcc attgccgatg cgcttctcgg aagtctggga ttgggtgaca tcgggcagca
      541 cttcccggat accgaagaaa agtggcgcgg gatttcgagt ctggagatat tgcggcaggt
      601 ggcggtcaaa gtgaccgccg gccacgggga agtcacaagc gttgatgcaa cagtagtggc
      661 ggaaaagcca cgtttggggc cttacattga aaagatgcgg gaaaagattg cggagacgtt
      721 aggcctcgcc gttagcgcag tctcgatcaa agccaaaacc gccgagggat tggggtcaat
      781 cggcgcggga gaagcgatgg cggcgtatgc ggtggcctca gtaaaccaaa agaaggtgga
      841 agatggtagg aggtaggtga tagggaaaga ctaagaatct tccctaccac atcccaccta
      901 tcacctatta cctgcatgga agctttctcc gcccgcgccc aagagttgct cgacttagcc
      961 acgagttatg gggtctggtg gctgattttc tgctatgtat ttttgtattt gagcgccatc
     1021 gtcgagtcgc ttttcccgcc ctatccgggg gacgtggtaa ccttcgcggg cgggtactta
     1081 gcttcgctgg ggaaactgaa ctttcccctg gtctttttcg ccaccagcct ggggagtctc
     1141 tccgggcttt tgctccttta cgctataggt caaaggcacg gacgccgact ctttcaaaag
     1201 gagcaaagct attggcggac gccggcgcat ttagccaaag tcgagcgctg gttcgcccgc
     1261 tacggggcca aggtgattat tgccagccgg tttttgagcg gcatccgctc cggcatcgcc
     1321 ttagcggcgg gaatcgggaa catcggctgg cgaaggatgg tcctctacgg aacggtctcg
     1381 attctattat ggaacggcct gattcttttt gtatcttcct ttgtggaaca gaactggcaa
     1441 aggctttacg gcctgctttt gctctacaat aaggttctgt tcggcggcct gctactgacc
     1501 gcgataggat tcggcatctg gtggtggcga aagaggcaag taccgaaagg agcggcgtga
     1561 agattttagt cttattgggc ggcgattcgg
//
LOCUS       DBNGZA010000297         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_480732, whole genome shotgun sequence.
ACCESSION   DBNGZA010000297 DBNGZA010000000
VERSION     DBNGZA010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_480732"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="AC16FU_06385"
     CDS             complement(<1..614)
                     /locus_tag="AC16FU_06385"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR2994034.1"
                     /translation="MTTKRIATFWTAAFFAAALVPRITWAGSFTKLKAGDGVEVSGTW
                     DPKGFFAAKSVEKLKEPRRPTLRGAIEKVGKSDSSFVLFGRKIHVNGKSAFWAKGKPV
                     GDFGRLKAGLRVEVTCNATEAGVWEGRKIEWDGVKPSDKIKGTLTRVAFHNQPPDTLE
                     IYGLKILVNNNTKLSGSTRYIEEELFSTLSPDEAEAYLPRLKLG"
     gene            complement(633..1181)
                     /locus_tag="AC16FU_06390"
     CDS             complement(633..1181)
                     /locus_tag="AC16FU_06390"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR2994035.1"
                     /translation="MGRTICLLLFTLLFLGGPAAAPPPPRPQYLKLKVGDTVEVKGNW
                     DRKQQIFVASEIEKLPAPRRPKLRGPIGKILLADKSFVLFGKKISTDTRTEFLDSGSQ
                     KVHFADLKASQRVEVTCNPDEKGNWTARKIAVEKVKPTDKIKATVTRLAFTGTPPDTF
                     EVSGLKILVGAGTEMFGPWPEK"
     gene            complement(1174..2523)
                     /locus_tag="AC16FU_06395"
     CDS             complement(1174..2523)
                     /locus_tag="AC16FU_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR2994036.1"
                     /translation="MLTAKRRLRGVRLLEPSPAYAHLSAATAAEVMQPIFQDELSGAW
                     NRPVRLGKFFIPRVIPIGGGKFLIQYRFSTVEATGPRDWIFFGRILAPDETVPTFVQK
                     PNAFYLAQFRLVVPIFPFDPKLKVLKKFFQPEEAPALLNSLQPVLNGRITIEKLDVLG
                     YRLELRSRSLLRLDLKQDSRTTTLVAKVMRPERAAQLFWHLQELERSGFHTRAADAIT
                     IPHPIAYAPEGIIWLEAVFDPSLHDVIGEEAFVSGCGAAARTLNKLKTAPLENLVPYT
                     LEEELLLLRQTGAEIAQIYPVLAERLQEVLALVLQNAPAARQNRPAPVHRDFYDKQLL
                     IGAARTTLIDADTLALSDPALDVGNFLAHLTLRAKQNPAFHVFVKNGRRAFTAEYRVK
                     DLSLPAEEFWRSVRWWEAAALLRLGCLYSLRPRWKNLAIPLLEQARKMLTHAGGIDG"
BASE COUNT          548 a          784 c          802 g          596 t
ORIGIN      
        1 tcccccagct tcaaacgagg caggtaggct tcggcttcat caggggaaag cgtgctgaaa
       61 agctcctctt caatataacg ggtcgagcca gacaatttgg tattgttgtt caccagaatt
      121 ttcaggccat aaatctcgag ggtatccggc ggctggttgt ggaaggccac acgggtaagg
      181 gtgcccttga tcttatcgga gggcttgaca ccgtcccatt ctatcttgcg gccttcccag
      241 acgcccgctt cagtggcgtt gcaggtgacc tccacccgca ggccggcttt cagccggcca
      301 aaatccccga ccggttttcc cttggcccaa aaagcagatt ttccgtttac gtggattttc
      361 cttccaaaca gaacaaagct ggagtcgctt ttgcccactt tctcgatggc cccgcgcagc
      421 gtcgggcgcc ggggttcctt cagcttttcg accgatttgg cggcgaagaa tcctttgggg
      481 tcccacgtgc cggatacctc cacgccgtcc ccggctttca gtttggtaaa agaaccggcc
      541 cacgttatcc gtggcaccag agcggcggcg aaaaaagccg ccgtccagaa ggtggcgata
      601 cgtttggtcg tcattctccc ctttctactg tcttactttt ccggccaggg gccgaacatt
      661 tccgttcctg ccccgaccag aattttcagc ccggagactt cgaacgtatc cggcggggtt
      721 cccgtaaagg ccagacgggt gacggtggcc ttgattttgt cggtgggctt tactttctca
      781 accgctattt tccgggcagt ccagtttccc ttttcatccg ggttgcaggt gacctccacg
      841 cgctggctgg ctttcaggtc tgcaaagtgg actttctggc tcccgctgtc cagaaattcg
      901 gttctggtgt cggtgcttat cttctttccg aagagaacga aacttttgtc tgccagcagg
      961 attttgccga tcggcccgcg cagcttgggg cggcggggcg cgggtagttt ttcaatctcg
     1021 ctggccacaa atatctgctg cttccggtcc cagttcccct tcacttccac cgtatcgccg
     1081 actttcagct tcaggtactg cggccgggga ggcgggggcg cagcggccgg tccgcccaga
     1141 aacaagagag tgaacagcaa aagacaaatg gttctaccca tcgatccctc cggcgtgagt
     1201 caacatcttt cgagcttgtt ccagaagcgg aatcgccaga tttttccatc ggggacgtaa
     1261 ggaatagagg caacccaagc gcaaaagcgc cgcggcttcc caccaccgaa cgctccgcca
     1321 gaattcctct gcgggcaaag agagatcctt cacccggtat tccgcggtga aagcgcggcg
     1381 accgtttttt acgaatacgt ggaaagcggg attttgtttg gcgcggaggg tgaggtgcgc
     1441 caggaaattg ccgacgtcca gagcgggatc gctcagggcc agcgtatccg cgtcaattaa
     1501 ggtcgtcctc gctgcaccaa tgagcagctg cttgtcgtaa aaatcgcggt gcactggtgc
     1561 cggtctgttc tgccgggctg ccggagcgtt ttgcagcact aaggctagca cttcctgcaa
     1621 acgctctgcc aaaaccggat aaatttgagc gatttcagcc ccggtttgcc ggagcagtaa
     1681 aagctcctct tccagagtgt aggggacgag gttttccagc ggagcggttt taagcttatt
     1741 caaggtccgg gcggcggccc cgcaaccgga aacgaacgct tcttctccga tcacatcatg
     1801 gagtgaagga tcaaagacgg cttccagcca gataatgcct tccggcgcat aggcaatcgg
     1861 gtgcggaatg gtaatggcat cggccgcccg ggtatggaat ccggagcgtt ccagctcctg
     1921 cagatgccag aagagctgcg ccgctctttc cggccgcatc actttggcca cgagtgtcgt
     1981 ggttcgggaa tcctgtttta agtccaggcg cagaagggag cggctgcgca gctccagccg
     2041 gtagcccaga acgtcgagtt tttcaatggt gatccggccg ttcaggaccg gttgcaaact
     2101 gttgagcagc gccggagcct cctccggctg gaaaaatttt ttcaatactt tgagcttggg
     2161 atcaaagggg aagatgggca cgacgaggcg gaactgcgcc aggtagaacg cgttgggctt
     2221 ttggacgaag gtgggcaccg tttcgtccgg ggccagaatc cggccgaaga aaatccagtc
     2281 tctggggccg gtggcttcaa ccgtggagaa acggtactgg atcaaaaatt ttccgccgcc
     2341 gatgggaatg acgcgcggga tgaaaaactt ccccagccgt accggccgat tccaggcccc
     2401 ggataactcg tcctgaaaga tcggttgcat gacctccgca gcggttgccg cgctcagatg
     2461 ggcgtacgcc ggactgggtt ccagcaggcg tacgccgcgc aggcggcgtt tcgcggttag
     2521 catatgaccg cgctccccgc tcggggaaac cgaccgggaa aagcacccat cgggcggacc
     2581 ggaagagcgg gtagcgagct acccagcggc gaccgccagg ggtacaaaaa aacctctttt
     2641 ttcgttctgg gagagccact cctggtagaa cgcaaaaaat tgtcctttcc gggccaaaag
     2701 ctccgcgggt gtgccggctt cgaccagtct
//
LOCUS       DBNGZA010000298         9809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1435994, whole genome shotgun sequence.
ACCESSION   DBNGZA010000298 DBNGZA010000000
VERSION     DBNGZA010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1435994"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="AC16FU_06400"
     CDS             <1..945
                     /locus_tag="AC16FU_06400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR2994037.1"
                     /translation="EKVTFRDLALVVIDEQHRFGVLQRAGLRKKGFLPELLVMTATPI
                     PRTLSMTLYGDLDVSVIDEMPPGRQKITTVWRQEPARPDILKFIADQVRKGFQAYIIY
                     PLVEESEKADLKAATESYHELQEKHFSDFNVGLIHGRLKAEERTRVMEAFRKREYQIL
                     VSTTVVEVGMDIPNATVMLVEHAERFGLSQLHQLRGRVGRGPARSYCILLSYGALSLE
                     AHQRLEILQQTEDGFKIAEADLKLRGPGEFFGTRQHGLPELKIADLIGDQKLLFDARR
                     WAQRLWQEDPNLAHPKHQRLREQFTRQYHIKLDYLRAG"
     gene            complement(959..2446)
                     /locus_tag="AC16FU_06405"
     CDS             complement(959..2446)
                     /locus_tag="AC16FU_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR2994038.1"
                     /translation="MKHGFGILGLALLLGLLGDLLLRATPWGVNIFFWAIALLLSVIL
                     LIHRWRVELAGDVLRFALPVVFFAFGFVWRDSLTLKALDALALLVILSLFTMRSQAIQ
                     LRLSGLVRYLEGFVLTGINLAAGIFPLVLADIAWKDIPRGGWSRQSVAVLRGLLIALP
                     LLLVFGSLLMAADAVFAGIIKNLLHFNLADVFKHIFVTGLAAWPVGGFLRGIFLADKQ
                     TMFSGELPKSPGLGIVEMGVALGSLNVLFLGFVFVQFRYFFGGAEVVTLTSGLTYAEY
                     ARRGFFELVVAAALVLPLLLFGHWLLRKDQPTYEKVFRLLAGMLIVMLFVVLASAFQR
                     MHLYQQEYGLTELRLYTTAFMGWLAVVFIWFGATVLRGKGERFAFGAVVAGFLTIVVL
                     SSFNPEALIIRTNAALAKTGKSFDVDYALSLSADAVPSLVAALPDLDPKDRCNVATLL
                     LKNWSPSTHTDWRTWNWGQARARRVVEKNQDDLQEMVKSWFIQNF"
     gene            2803..4647
                     /locus_tag="AC16FU_06410"
     CDS             2803..4647
                     /locus_tag="AC16FU_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2994039.1"
                     /translation="MESNQLIGQMLGKYRIDAKLGEGGMGVVYRATDITLDRVVALKM
                     LQKEITVDPKIKERLKIEAKALARFNHPNIAILYEFDVVNNFFAMEYIEGQTVEELCK
                     QEGVLPPERIRSILKQVLPALGVAHKSGIIHRDIKPANLMLTPSGLVKITDFGIAKMP
                     AGVSGVKTMSGERIVGSYYYISPEQIQHMPVDGRTDIYSLGVTLFEMATGKVPFDSDS
                     DFLMMKAHVEDPPPLPSSLNPHLDPRFEKVILKALEKDPKNRYQSAEEMLVALDAAVL
                     SPKPVPNPPGKLAEERKSASRRRRWLVPVGLLFVVGLIAAAVMVFSKGKNNGTPEFFG
                     GAEPSAQITLSDVPAGGQVFLDDEKVLVTPDQPLELAPGTHRLRVEKSGALPWNRKFE
                     VEPGQRLAFSVALAVNQGFLRVVTNPPEAELSLDDLPIKNVAEALPVTVGAHRLKAQA
                     PGFRDTIAEFTVESGETKSVALTLNSRGEFLRITNLPPEAYVWVNGQEVRNAGEPLRV
                     PFGENRIHVRVPEREDWSRLVMVPAGKMVSVEYQPPKGMLTLSARPYATFYVDGQKIG
                     ESGVKTQLEVEAGTHQVRLVYLDGSFDTTVVVSSKTPTEVNHTFRVRR"
     gene            4663..5691
                     /locus_tag="AC16FU_06415"
     CDS             4663..5691
                     /locus_tag="AC16FU_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2994040.1"
                     /translation="MLRIDSRLRKTALWLVSAHFFISTVIFAFPAGSLAQGAGELEQA
                     QNAYYDGDFDRTINLLTGNVTSPRLTDAQVDTAYQFLGSAYFLKGQAAQAQNIFRDFV
                     TFNPRAALDESFFPPEVVSAFAAVKAQYLASLGTAGFLRLAGQPKEAELKIDGKRQSL
                     AAEPLKVLPGRRTIEVLQNGQVIYTGLVDIRTGETITVDTSLKRIMPGLLALQVVPPD
                     ALIFIDGVAVSLVNGRAELPAGGHTLKATKIGYQPYDGKFTLSSGEQTSLTINLEPAS
                     QPATPALALNATPKPSKPLFKKPIFWGILGAAVIGGVVVAAGGGGGRGTDGGTTALPP
                     PPPPPGKR"
     gene            5734..8652
                     /locus_tag="AC16FU_06420"
     CDS             5734..8652
                     /locus_tag="AC16FU_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994041.1"
                     /translation="MQQWKRFWLTCTVLTVWGWLASNALAEPQKPLWGQDYLAYDGRV
                     AAFAADYTTDGSIYAVVVLAASPDSVVLLKSDNGGACWSEIGGRLVASGDSISEVRLA
                     TIEYGTVKGLLIFSLTRLGGGVLRATRFSTAGVFNAQSTVLTGILGDTLERFSVEPNL
                     ERGDTLVLAFGTKKGSVGVTRSVNAGATWGAYRLLVVGFTGLPDLAYGAKGAYFLACY
                     NSSTGEIQTFVSRDYGLTWTGPAAVARDTLRLALDLQIMAEHDANVDSTALWIFTNRL
                     ETDALLCGAGISFCFQLDLFFDASRDAGGKWSADSNLTSTPCCCNAGATVGYHEILPR
                     LAVSKVKPTRVSDLTYILVGGACSGVSAVEHLSSTVDAPDIWTVPQAVNVNGINEVIP
                     AQIVYSNAAARNGPAIFYAGKSGQGLYLQAGWLAATAGPVVCTRVAATASVGQGFDVV
                     ATVTDTAGLAQVKLFYRIIGRNAFGSIPMTGSSNTYIATIPAESVTVRGLEYYVEATN
                     NNSQTTLQPASAPSVPAKVAAVITNLVSPVFLPAAAYRMVAFPFLVYDSAATGTLFDD
                     LGSYNNTLWRLFRWNPTSATPAYDEYPALPPFAAGKGFWLISRNPAQFDASGISTVSD
                     NTNHVDVSLDRGWNQISSPYFWNVKIDSVRVDTGGAFTASFQQAVNRGILDSTMWELN
                     LSGSGYLRTHDLSPWSGAWIFNQRNYPIRLRVPAREQAGLGKIALAAPPPGWQVSVAL
                     SAGQYEDRENLFGVHPDARPGRDKFDLGEPPPIGPLVSLAFENSGAVLAADIRPPFTD
                     GGRWEMVVKNPQVNSPVRLEFAGVEGISPNFAAILFDPISGTKTDLRQTSVFTFEFTP
                     EQSERRFMILVGTPKFVATTSLVPYRFALEQNYPNPFNPATAIAYSLPVQQQVSRLVF
                     NILGQKVRTLVSGLEQAGPHQVTWNGKDDAGTEVASGIYFYRLAAGDERETKKMLLVR
                     "
     gene            9031..9777
                     /locus_tag="AC16FU_06425"
     CDS             9031..9777
                     /locus_tag="AC16FU_06425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2994042.1"
                     /translation="MALPLEGKSALVTGSAWGIGRATALALADAGAGVVINYRTSEKA
                     ALEVVDAIRKKGGKSIAVQANVAEPGQVELLFERAQKELGTLDILVNNVGAFLLKPLS
                     ETTFTEWTALITSNLHTTFLCSKAALPGMRAKNWGRIINISFATADKITSAKRVSPYQ
                     IAKTGVLIFTKTLAAEEVKNGITVNSVGPGFVDTGRIAEAFREQMLKLSPLDRLITPE
                     EVARAVVFLTSPESAGITGTHLTVAGGWQV"
BASE COUNT         2137 a         2833 c         2960 g         1879 t
ORIGIN      
        1 gagaaagtca cttttcgtga cctggcttta gtggtgatcg acgaacagca ccgctttggt
       61 gtgctccagc gggcggggct gcgcaagaaa ggattcctgc cggaactttt agttatgacc
      121 gctacgccaa ttccacgcac tttatccatg accctctacg gcgacttgga cgtctcggtg
      181 attgacgaga tgccaccggg gcggcagaaa atcaccaccg tctggcggca ggaaccggcc
      241 cgtcccgaca ttttgaagtt catcgccgat caggtgcgga aagggtttca ggcgtacatc
      301 atctatccct tggttgaaga atctgaaaaa gccgatctca aagcggcaac ggaaagttac
      361 cacgaactgc aggaaaaaca tttttctgat ttcaatgtcg ggcttattca cgggcggctc
      421 aaagcggagg agcggacgcg agtcatggaa gctttccgca agcgggagta tcaaatcctc
      481 gtctccacga cggtggtgga agtggggatg gacattccca acgccacggt aatgttagtg
      541 gagcatgccg agcgtttcgg tttgtcgcaa ttgcaccagc tgcgcgggcg ggtggggcgc
      601 ggcccggccc ggtcgtactg cattctgctt tcctacggcg ccctttcgct ggaagcccac
      661 cagcggctcg agattctgca gcaaactgag gacgggttca aaatcgccga ggccgattta
      721 aagctgcgcg ggccggggga gttcttcggg acgcgacagc acggcttgcc ggaattgaaa
      781 atcgccgacc taatcgggga tcaaaaactt ctatttgacg cccggcgctg ggcgcagagg
      841 ctctggcagg aagacccgaa cttggctcac cccaaacacc agcgcctgcg ggagcagttc
      901 acccgccagt accatataaa gttagattac ctgcgggctg ggtgaatctt ttcaaggttc
      961 aaaagttttg gataaaccag gactttacca tctcctgcaa atcgtcctga tttttctcta
     1021 ctacccggcg ggctctcgcc tgaccccagt tccaggtgcg ccaatcggtg tgcgtggacg
     1081 gagaccagtt tttgagcagg agggttgcca cgttacagcg atctttgggg tcaaggtcgg
     1141 gcagagccgc caccagggag ggaaccgcgt ccgcgcttaa tgagagggca tagtccacat
     1201 cgaagctctt gccggttttg gccaaggcgg cgttggtccg gatgatgagt gcctccgggt
     1261 tgaaggaaga aagcacgaca atagtcagaa aacccgccac cactgcacca aaagcaaatc
     1321 tttccccttt tccccggagc acagtagcac caaaccagat aaagaccacc gccaaccagc
     1381 ccatgaaggc cgtagtgtag agccggagtt cagtcagccc gtactcctgt tgatagagat
     1441 gcatgcgctg gaaggcggaa gccaggacaa caaagagcat gacaattaac atccccgcca
     1501 gaaggcggaa taccttctca taggtgggtt ggtctttacg caagagccag tgcccgaaga
     1561 gcaaaagcgg caagactaaa gcggcagcga ccaccagttc gaaaaatcca cgccgggcgt
     1621 actcggcata agtgagtccg gaagtgaggg tgaccacctc ggcaccgccg aagaagtaac
     1681 ggaactgcac gaacacgaaa ccgaggaaaa ggacattcag cgaacccagc gccacaccca
     1741 tttccacaat gcccagcccg ggggatttgg ggagttcacc gctgaacatc gtttgtttgt
     1801 cggccaaaaa gatgccgcgc aagaacccgc cgaccggcca cgcagcgagg cccgttacaa
     1861 agatatgctt gaacacatcc gcaagattaa agtgcagaag gttttttatt atccccgcga
     1921 aaaccgcgtc ggctgccatt aggagacttc cgaatacgag cagcaacggt aaggcaatga
     1981 gcaagccgcg caatacggcc actgactgcc tggaccatcc gccgcgcgga atatccttcc
     2041 aagcgatatc ggccaaaacc agggggaaaa tcccagcagc caggttgatt ccggtgagaa
     2101 cgaagccctc caggtagcgt accaagccgg aaaggcggag ctgaatcgcc tgtgagcgca
     2161 tcgtaaaaag cgacaggatg accagaagtg ccaaggcgtc cagagccttt agagtcaatg
     2221 agtcgcgcca gacgaaaccg aaggcgaaga aaacgacggg tagagcgaac cggagcacat
     2281 ccccggccag ttcaacacgc caccgatgga tgagcagtat gaccgagagc agcagtgcaa
     2341 tcgcccagaa aaaaatgttc acgccccagg gcgtggcgcg gagcaatagg tcgccgagta
     2401 ggcccagcaa aagagccagt ccgagaatgc caaatccgtg tttcatccgg tcgttcattg
     2461 gcgacctgct ctcccattat acgttgggta aagacgttca attaccttat cggcgagaga
     2521 tggcatttgg gtagtcaggc ttatttctac gttagttctc aaatccaaca attctacatg
     2581 gattgctgac ccctccctac cctcccctcc aaggggaggg aaaagcaggg ctgacctctc
     2641 cccaccttcc tccgccgcgg ctggatcttc gacccgcaaa ggagaggaac agtttctccc
     2701 tcacaaataa actgccagca aacaaacttc cctatcaggg cgaaaccgct gattccctgc
     2761 aaagtaacgt ctttcggctt tgcgtcgata atccgaaata aggtggaaag taaccagcta
     2821 atcggccaga tgctcggcaa gtaccggatt gatgccaaat taggcgaggg ggggatgggt
     2881 gtggtctacc gggccaccga catcaccctg gaccgggtgg tggcgctcaa gatgctccag
     2941 aaggagatca cggtcgatcc gaaaatcaaa gagcggctga agatcgaggc caaagcgctc
     3001 gcccgcttca accaccccaa catcgccatc ctctacgagt tcgacgtcgt gaacaacttc
     3061 tttgccatgg agtacatcga aggccagacg gtggaggagc tgtgcaaaca ggagggtgtt
     3121 ctcccgccgg agagaatccg ctccatcctc aagcaggttt tgcccgcctt aggtgtggcc
     3181 cacaagtcgg ggatcatcca ccgcgacatc aagccggcca acctgatgct cacccccagc
     3241 gggctggtga agatcaccga tttcgggatc gccaagatgc cggcgggcgt ctccggcgtc
     3301 aagaccatgt ccggcgaacg gatcgtcggt tcctactact acatctctcc ggagcagatc
     3361 cagcacatgc cggtggacgg gcggaccgac atttactctc tgggcgtcac ccttttcgag
     3421 atggcgacgg gaaaggtgcc gttcgattcg gacagcgatt ttctgatgat gaaggcgcac
     3481 gtggaggacc cgccgccttt gccttcttcc ctcaatcccc accttgatcc ccgctttgaa
     3541 aaagtgattc tgaaagcgct ggaaaaggat ccgaagaatc gctatcagtc tgcggaagaa
     3601 atgctggtag ctctggatgc agcggtgctg agccccaagc cggtgccgaa cccccccggg
     3661 aaattggcgg aggaaaggaa aagcgcttcc cggcggaggc gctggctggt tccggttggc
     3721 ttgctcttcg tggtcgggct gattgcggcc gcggtcatgg tcttctccaa aggaaagaac
     3781 aatggcactc cggagttttt cggcggcgcc gagccctccg cccagatcac cctgagcgac
     3841 gtgcccgccg ggggccaggt cttcctcgac gatgaaaagg tcttggtaac tccggaccag
     3901 ccgctggaac tggcccccgg aacgcaccgg ttgcgggtgg aaaaatcggg cgcgcttccc
     3961 tggaacagaa aattcgaagt ggaaccgggg cagcgcctgg cctttagcgt ggctctggcc
     4021 gtcaatcagg ggttcctgcg ggtggtgacc aatccgcccg aggccgaatt aagtctcgac
     4081 gatctgccga taaaaaacgt agccgaagcg ctgccggtca ccgtgggagc ccaccggctg
     4141 aaagcccagg cgccggggtt ccgggataca atcgcggagt tcaccgtcga atcgggcgag
     4201 acgaagagtg tggcgctcac cctcaactcg cggggcgagt tcctccggat cacgaatctg
     4261 cccccggagg catacgtctg ggtgaacggc caggaagtac ggaacgcggg ggaaccgtta
     4321 cgggtgccgt tcggggagaa ccgtatccac gtgcgggtgc cggagcggga agactggtcg
     4381 cgactggtga tggtcccggc agggaagatg gtgtcggtcg aataccagcc ccccaagggg
     4441 atgctcacgc tttcggcccg gccgtacgcg acgttttacg tcgatggtca gaagatcgga
     4501 gaatccgggg taaaaacgca gttggaagtg gaagcgggaa cccaccaggt acgcctggtg
     4561 tacctggacg gcagcttcga caccacggta gtggtgagca gcaaaacgcc cacggaagtc
     4621 aaccatacgt ttcgcgtgag gcgatagcga aaaggaggca aaatgctacg gatagattcc
     4681 cggctcagga aaacggcgct gtggctggtg agtgcacatt ttttcatcag cacggtcatt
     4741 ttcgcatttc cggccgggtc tctggcgcag ggggcggggg aattggagca ggcgcagaat
     4801 gcgtactacg acggcgattt tgaccgcacc attaatcttt tgaccggcaa cgtcacctcc
     4861 ccgcgcctga ctgacgccca ggtcgatacc gcttaccagt ttctgggcag cgcttacttt
     4921 ttgaaagggc aggcggcaca ggcgcaaaat attttccggg atttcgtgac gttcaacccc
     4981 agagcggcgc tggacgaaag ctttttcccg ccggaagtgg tttcggcctt cgctgcggtc
     5041 aaagctcaat acctggccag tctgggcaca gccgggttcc tgcgcctggc gggtcagccg
     5101 aaagaggcgg agctcaaaat cgacggtaaa aggcaaagtc tcgcggctga acctttgaaa
     5161 gtcctgccgg ggaggcggac catcgaggtc ttgcagaacg gccaggtgat ctacaccggt
     5221 ctggtcgaca tccgaaccgg tgagacaatc accgttgata ccagtctcaa aaggatcatg
     5281 ccgggattgc tggcgctgca ggttgttccc cccgacgccc tgattttcat cgatggtgtg
     5341 gcggtctccc tggtcaacgg ccgggccgaa ctccctgctg gcggacacac gctgaaagca
     5401 accaagattg ggtaccagcc gtatgacgga aaattcaccc tgagctcggg cgagcaaacc
     5461 agtttaacaa taaatctcga gccggccagc cagccggcca ctcctgcttt ggcgctcaac
     5521 gcaacgccga aaccttccaa gccacttttc aaaaagccta tcttctgggg cattctgggg
     5581 gccgcggtga tcggcggcgt ggtggtcgcc gcgggtggcg gaggaggccg ggggaccgat
     5641 gggggtacaa ctgctttacc gccgccgcca ccgccgccgg ggaagcgcta agtccggact
     5701 tctgcaatct gttggtaaaa ggtggaggga accatgcagc aatggaaacg attttggctc
     5761 acgtgcacag tattgacggt ctggggttgg ctcgcgagca acgcactggc ggaaccccaa
     5821 aaaccgcttt ggggacagga ctacctggcc tacgacggcc gcgttgccgc ttttgccgcc
     5881 gattacacca ccgacggttc aatatatgcg gtcgtggtgc tggcggcttc tcctgattcc
     5941 gttgtcctct tgaaatcgga caacggcggc gcctgttggt cggagatcgg cgggcggctg
     6001 gtggcgtcgg gcgattcgat cagcgaggta cgcctggcca ccatcgagta cggcaccgtg
     6061 aaaggacttt tgatcttcag tctgacgcgg ctggggggcg gagtgctgcg cgccacccgt
     6121 ttttccaccg cgggagtatt caacgcgcag agcaccgtcc tgacgggaat tctcggagat
     6181 accctggagc gtttctcggt ggagccgaat ctggaacggg gggatacgct ggtcttggca
     6241 tttgggacga agaaggggag cgtgggggtg acgcgcagcg tcaacgcagg agcgacctgg
     6301 ggcgcctacc gcctgctcgt cgtcggcttc accggcctcc cggatctcgc ctatggcgcc
     6361 aaaggcgcct actttctcgc ctgttacaat tcgtcaaccg gcgaaattca aacgtttgtc
     6421 tcccgagatt acggtttgac ctggaccgga ccggcagcgg tggcccgcga taccctgcgg
     6481 ctcgccctgg atctgcaaat tatggccgag cacgacgcga acgtcgattc caccgccctg
     6541 tggattttta ccaaccggct ggaaaccgac gcgctcctct gcggggccgg cataagtttc
     6601 tgtttccagt tggatttgtt cttcgatgcc agccgcgacg ccggcggcaa atggagcgca
     6661 gattccaatc tgaccagtac tccctgctgc tgcaacgccg gcgcgacggt agggtaccac
     6721 gaaatcctcc cacggctggc cgtctccaaa gtgaagccga cccgggtgag cgacctgacc
     6781 tacatcctgg tggggggcgc gtgcagcggg gtttcggcag tagagcacct ctcctccacc
     6841 gtggacgcac ccgatatctg gacggtgccc caggcggtga acgtcaatgg catcaatgaa
     6901 gtgattcccg cgcagattgt ctactccaac gcagcagcgc ggaacgggcc ggcaatcttt
     6961 tatgccggga aaagcggcca gggactctac ctgcaagccg ggtggcttgc tgccaccgcg
     7021 gggccggtag tttgcacccg ggtcgcggcc accgccagcg tggggcaggg tttcgacgtg
     7081 gtggcgacgg taaccgacac agcggggctg gcacaggtaa aactcttcta ccggattatt
     7141 gggcgcaatg cctttggcag cattcccatg accgggtcga gcaacacgta cattgcgacc
     7201 attccggcgg aatcggtgac ggtgcgcgga ctggaatatt acgtggaagc gaccaacaac
     7261 aacagccaga ccaccctgca gccagccagc gcccccagcg tcccggctaa agtggcggcg
     7321 gtcattacca atctggtgag ccccgtcttc ctgcccgcgg cggcctaccg gatggtggcg
     7381 ttccccttct tagtttacga cagcgccgcc accgggactt tgttcgacga tctcggcagt
     7441 tacaataaca ccctctggcg gcttttccgc tggaatccca cctccgccac cccggcgtac
     7501 gacgagtatc cggctctgcc gccttttgcg gccgggaaag ggttctggct catcagccgg
     7561 aatccggccc agtttgacgc ctcaggcatt tccacggttt cggacaatac caaccacgtc
     7621 gatgtctccc tcgaccgcgg ctggaaccag atcagttcgc cttacttctg gaatgtcaag
     7681 atcgactcgg tgcgggtcga taccggcgga gcgtttacgg ccagttttca gcaggcggtg
     7741 aaccggggga ttctggacag caccatgtgg gaattgaatc tctccggctc cggttacctg
     7801 cggacgcacg acctttcgcc ctggtccggc gcctggattt tcaaccagcg caactatccg
     7861 attcgcctgc gcgtgccggc acgggagcag gccggtttgg ggaagatcgc cctcgctgct
     7921 ccccctccgg gctggcaggt aagcgtagcg ctctccgccg ggcaatacga agatcgggag
     7981 aatctcttcg gcgtgcaccc ggatgccaga ccggggcggg acaagttcga cttgggcgag
     8041 ccgcccccca tcggccctct ggtttcttta gccttcgaaa attccggggc cgtcctggca
     8101 gccgacattc gtccaccctt taccgacggc gggcgctggg agatggtggt caaaaatccg
     8161 caggtgaact ctccggtgcg tctggaattt gcgggcgtgg aggggatatc ccccaacttc
     8221 gcggccattc tttttgatcc catctccggg acgaagaccg atttgcgtca gacgtcggtt
     8281 ttcacgtttg agttcactcc ggaacagagc gagcgccggt tcatgatttt ggtcggtacg
     8341 ccgaagtttg tggctacgac gtcactcgtc ccgtatcgct ttgcgctgga acagaactac
     8401 ccgaacccct tcaatccggc aacggcgatt gcttactcgc tgccggtaca gcagcaggtc
     8461 tcgcgtctgg tgttcaacat cctagggcag aaagtgcgga cgctggtttc cggtctcgag
     8521 caggccgggc cgcaccaggt gacttggaac ggaaaggacg acgccgggac ggaagtcgcg
     8581 agcgggatct acttctaccg gctggcggca ggggacgagc gcgagacgaa gaagatgctt
     8641 ttggtacgtt gagaagattg aagtcgtcgg gctcggggac gagcccgact acccctctgg
     8701 gtttaacctc accctaacag tcttctacga gaagaggggg aaagatgttg acccctccct
     8761 ggccctcccc tcaaagggga gggcattttc tgtgctcctt ctcctttcaa gaggggatgt
     8821 gagcaactgc tgaccccgcc ctaccctctc ctcaaggaga gagagttaaa cttcttcccc
     8881 tgagcaaaag agaaagagga gatgaagttc cctcccctgg aggggagggg ggtgaggtca
     8941 tatgagcatt tctgtaacgc tggtttcaaa accggcgttt ttttgtagat tcccttcagt
     9001 aaagaagcgt cccgaggaga gggagatagt atggctctac cattggaagg aaaatcagca
     9061 ctggtgaccg gcagcgcctg gggcattggc cgggctaccg ccctggcctt ggccgacgcc
     9121 ggggccggtg tcgtcatcaa ctaccgcacc agcgaaaagg cagcgttgga agttgtagac
     9181 gcgattcgca aaaagggagg caagtcgatc gccgttcaag cgaacgtggc cgagccgggg
     9241 caggttgagc tcctctttga gcgggcgcaa aaagaattgg gcaccctcga catcttggtg
     9301 aacaacgtcg gcgcgtttct gctcaagccg ctttcggaga cgacatttac tgagtggacg
     9361 gcccttatca cttccaacct ccacacaacc ttcctctgct ccaaagccgc actgccggga
     9421 atgcgcgcca aaaactgggg acggatcatc aacatcagtt ttgccacggc ggataagatc
     9481 acctccgcca agcgggtctc tccttaccag attgccaaga cgggagtgct cattttcacg
     9541 aaaacgctcg cagccgaaga agtgaaaaac ggcatcaccg tgaactcggt cggcccgggg
     9601 ttcgtcgata ccggccgcat tgccgaggca ttcagggagc aaatgctcaa gctctcaccg
     9661 cttgaccggc tcattacccc ggaagaggtg gcgcgggcgg tggtctttct gaccagtccg
     9721 gagtcggcgg ggattacggg gacgcatttg accgtggcgg gggggtggca ggtgtaaagg
     9781 cagaacacag tagacagaat acagcacac
//
LOCUS       DBNGZA010000299        23126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1751453, whole genome shotgun sequence.
ACCESSION   DBNGZA010000299 DBNGZA010000000
VERSION     DBNGZA010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 23126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23126
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1751453"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16FU_06430"
     CDS             complement(<1..425)
                     /locus_tag="AC16FU_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR2994043.1"
                     /translation="MDFRDPKNQKMILAILGLFFLGYVWYVRIYDKTAAALKVKRAEY
                     ERLMADLKAVELKAKSFEGLKTEYQDLLGRYQSIELLLPEEKQVPALLQQLNSAGAGL
                     QVRVVEVTPQGTSPAGFYNTAAFTLSLRGSYHAIGNFLAA"
     gene            complement(468..1166)
                     /locus_tag="AC16FU_06435"
     CDS             complement(468..1166)
                     /locus_tag="AC16FU_06435"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR2994044.1"
                     /translation="MIEINLLPKDLRKRSHVIRLPRTAWYVSVGVGALLVAVVGATVM
                     QRYQMKKWQTRIQKTRTQVEQMRRDIQVVDALEEVKAKILDRLSAIEQLDRNRAVYVS
                     MFEDLARRVPEYLWLTTFKEGLAQPAAAAPGGNAPPTAPAGAPSPGASAGSTTPQRTK
                     MEGFAYSLNSIATFLIQMKKSNFFKNVELEYAREQEVEGHLAYNFQLSCDLELVQSQP
                     VEPVEPAPISAVKP"
     gene            complement(1169..2227)
                     /gene="pilM"
                     /locus_tag="AC16FU_06440"
     CDS             complement(1169..2227)
                     /gene="pilM"
                     /locus_tag="AC16FU_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR2994045.1"
                     /translation="MITDLLRRSQNVAALDIGSGAIKLVQLEKKSGGYSLKSLGVREL
                     PLEAIVADEIKDRDTVIFNIQSLVDQCDPHTKEVVVSLAGHGVITDKISIDKKSGSEA
                     EQAILFEAEQRAPFDVEDVTLDYHVIAVNEETNKMDVLLVAARNEYLRSFLDVIADAG
                     LRAVVVDIDAFAILNAYEANYEIDPQKVTALVNIGFDATNITFLKDGQYHSTREVSAG
                     GRIIYDAIQKEFRLNHELTAKAIRGELEGTVDADMLRVTVISAAEELLSGLEVAFSYF
                     KSSAKVPAVEWIMLSGGGALVPFLPEFMQSKFATPVEIANPLRNIDYDPDQFRHLDLE
                     RIAPLLMVPIGLAARRVK"
     gene            complement(2513..2992)
                     /locus_tag="AC16FU_06445"
     CDS             complement(2513..2992)
                     /locus_tag="AC16FU_06445"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR2994046.1"
                     /translation="MRKLRKNRGFTFVELIVAVAVVGVLAALAAPRFFRTMTQLKAKG
                     AARDAVSVLRLARSQAVSKKIRYGILFELDNRRITYFRDDNSSGTYDTGDSLLTREVL
                     STDVTLSNSTLTNNTVVFTSTGQATGSGAVRVQTNDGTWRYAVDVLASTGRVRLTAL"
     gene            complement(3011..4570)
                     /locus_tag="AC16FU_06450"
     CDS             complement(3011..4570)
                     /locus_tag="AC16FU_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR2994047.1"
                     /translation="MRKVLMNQTGAAMLVAMVVLVMLAFIGIAAVTTSLTDSDISHNY
                     QKKTEAFYTAEAGLEYGLKTMQNNVTILDSAQLMNMINASNTLGRGTFTVTMGNDLPV
                     RTLISSGRNTDATSAQAVDVQYKLNPLSVWNNVLFAGVGQSGKAINGNVGFHGSVHIQ
                     GNGDRFTDTNGNGIHDPLEPYTDDNGDGVYTAPLTSSDVALDIGGGAGVYNNYNGMPV
                     SLSSRVPALGQVPFGGENVSSLDAEFRVQHGLVTINSNASQIGEPDVSGGSPPVKETM
                     DGVYVNDGFGGSNPTNVYADNGTSEKYDLGGGQLSFPNLNDPYTDGGVTYANYTAYLL
                     AHALVIGSDLILEAGTAMAKITGPNGSSIELDDQGHLNISGIVYVTGNITIQQGKGGS
                     GNNPIIYDGRGTLAARNDISITNNLLSKGAFPTDDVIGLVAGHDMTIAGSGASHLELT
                     GAFFAQHQMNNSKQNELAGAMVSNYFNITNVPHFYQVPSLKDNLPPGMPGAGTVTTYT
                     WKKRFASWREL"
     gene            complement(4649..5215)
                     /locus_tag="AC16FU_06455"
     CDS             complement(4649..5215)
                     /locus_tag="AC16FU_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR2994048.1"
                     /translation="MKKPKLFHWAQKWTEIYRSQRGFSIIEMLIVGFLTALLAGFTLE
                     FYLTQHNQWLTQTQLADLQQNGRNSMDALTGTIRSAGYGVLFGAPAFQVNTNQDTLAI
                     YAQNGAATDTTRFFVDRSIANNPRLMRKINSLESQVFAEYVDTVGFVPGGTNTLTVTL
                     VAREQMLKGNQYVNYTRRLVSSVKLKNR"
     gene            complement(5542..5814)
                     /locus_tag="AC16FU_06460"
     CDS             complement(5542..5814)
                     /locus_tag="AC16FU_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994049.1"
                     /translation="MRWVVGVIWILSESFLATLGRGEKTSWRTEMTITENREMQDKPG
                     ERSQKLDSVRSWPPHAGRLVKKAAPLPGFRPNFFQMVTRFRTGERV"
     gene            complement(5824..6210)
                     /locus_tag="AC16FU_06465"
     CDS             complement(5824..6210)
                     /locus_tag="AC16FU_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR2994050.1"
                     /translation="MLRLKNKRGFSLVEVLSAMVVLAIGILGLAPLIVLSIRTNSYSQ
                     DVTDASVFAQDRIEQLKSQASFTPMPYTETTNPGGGLTRTVRIDNNATDGTCPAGMYR
                     IAVTITWTEQNNVTRRVVFNTYKPVS"
     gene            complement(6445..7806)
                     /locus_tag="AC16FU_06470"
     CDS             complement(6445..7806)
                     /locus_tag="AC16FU_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2994051.1"
                     /translation="MPAVLVIDDKDSMRNMLSQTLVADGFEVETVPSGPQGIERAREK
                     RFDVVLTDLKLPDMDGLQVLIKLKEIDPDLSVIVMTAYGTIETAVQAMKEGAYDFLTK
                     PFDTDHLGVLIRRALENRRLLAENILLREELAHSLGYAEIIGKSEKMVEVSHLVQKVA
                     PSDTTVLLLGESGTGKELFARAIHNLSLRKNAPYVAINCAAIPSELLENELFGSEKGA
                     FTGAWARKMGKFEIAEGGTIFLDEVGDMSVALQAKLLRVLQEKTFERLGGNKTLSVDV
                     RVIAASNADLKAAIDKKQFREDLFYRLSVFPLAVPPLRERREDIPLLAEHFIAKYCAE
                     MKKPKKTLSSETLSVLDRYHWPGNVRELENTIERAVILCEGKRITPEHLAIRIPSVQE
                     IRLREGAGLKEVGAHAQMEAERALIQRVLNQTRGNKRKTAEILQIDYTTLFDKMKKYA
                     IEF"
     gene            complement(8024..8581)
                     /locus_tag="AC16FU_06475"
     CDS             complement(8024..8581)
                     /locus_tag="AC16FU_06475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR2994052.1"
                     /translation="MPFQFKVFLFIVASVGIAWVSRASVRDPHSHGFYRFFAWEAILV
                     MILLNLNYWFYAPFSIHQIASWVLLIVSTFLVIHGARLLRLVGKPASQRNDPALIGIE
                     KTTELVTVGAYRYIRHPIYSSLLFLAWGVFFKHPSWVGVSLAVITIFFLTMTAKMEEA
                     ENLRFFGATYQRYMKQTKMFIPFLF"
     gene            complement(8810..8882)
                     /locus_tag="AC16FU_06480"
     tRNA            complement(8810..8882)
                     /locus_tag="AC16FU_06480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8847..8849),aa:Thr,seq:cgt)
     gene            complement(9000..9683)
                     /locus_tag="AC16FU_06485"
     CDS             complement(9000..9683)
                     /locus_tag="AC16FU_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR2994053.1"
                     /translation="MATRSSGWAGNARAVLAKDLRSELRTRYALNTILLFGLVTVVAE
                     SFAIGQVRLEPDVLAALLWIAIFFAAMSGLAQVFVKEEEARTANTLKLAAAPEAVFLG
                     KLGFNFLLLLGLEIIILPLFFLLFNPALGNLPLFLVVTFLGTCGLVAATTIIAAIIAK
                     ASVKGALFAALSFPIVLPLIIVSITATRIALEGGSFAEGWDYIRMLIAYLGVMLVGSY
                     LLFEFVWYE"
     gene            complement(9652..10266)
                     /gene="ccmA"
                     /locus_tag="AC16FU_06490"
     CDS             complement(9652..10266)
                     /gene="ccmA"
                     /locus_tag="AC16FU_06490"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR2994054.1"
                     /translation="MTLSFSGLSKSFGFRVVFENISTTVAHGSVLVVTGPNGSGKTTL
                     LRIIAGLLRPSAGRITLALDSGEIPKEELRRHLALVAPDLQLYDELTALENLAFLTRL
                     RGFKHPQAHLEMALQEVGLAERGGDLVKAYSSGMKQRLQYAYARLLEPTLLLLDEPTA
                     NLDDAGKQMAHAFIQRQKERGIVVIATNEKEETRYGDQVIRLGR"
     gene            complement(10273..11133)
                     /locus_tag="AC16FU_06495"
     CDS             complement(10273..11133)
                     /locus_tag="AC16FU_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain c-type cytochrome"
                     /protein_id="HIR2994055.1"
                     /translation="MLPKLTDVSALATCQSCHGTDFKGGSSGISCVGCHNTQRNGRQC
                     IACHGGGGGNTTGAPPVNVAGRGDTVATVANDSLREVDSTRGVGAHRSMVLGRKFFSG
                     FDCAYCHVKPEFVIAPGHVTNSSGGRGDVVLSQFASLRAQIDTLPPAQFSLDSLACQN
                     VYCHGNFRGGNNVTTHFYRKDQALCGSCHDAGANPAALGGKHGFHVDPLGPNLSCATC
                     HDQVVTEAIPPTIPSIRTLALHVNGLYDIAFNSGGRGYIWDPAAKTCGGSNTHCHRNP
                     PTDLRNWYAP"
     gene            complement(11318..12229)
                     /locus_tag="AC16FU_06500"
     CDS             complement(11318..12229)
                     /locus_tag="AC16FU_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994056.1"
                     /translation="MKRILGSFLLIGLGSSVAVVYLQSFGALGATKSPIIFDHKKHLQ
                     RVGLKCLACHEPVQTSEAAADRLNPKEAVCLSCHNGPVAAKECKTCHINPDKPVTYLA
                     IKREIIFSHKKHIEQKTACQTCHANVERTVVLTAANMPEMAVCLSCHTGEKLSNACAT
                     CHSEPQQMMAVLHPANWIQIHKFKANLDGDYCASCHRGQDLCQSCHRGDNYTGSIHPL
                     NFKFTHGLEAKGKTNLCASCHETQSFCVACHQQELRMPLDHSDPLWVNRGDVRARNLH
                     AAAARRDLERCAACHDQSQPNCAACHG"
     gene            complement(12236..13519)
                     /locus_tag="AC16FU_06505"
     CDS             complement(12236..13519)
                     /locus_tag="AC16FU_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994057.1"
                     /translation="MRQNFLRALFWGSLLVLTRGAVAPAADFSGTLATSVYSFERITS
                     TEEIPQSRGNQTFTLFANDLTSGRGGLLSGHMFLRFRTDWQNALPGDPQWNFYNLYFD
                     YRTKSQKLAARVGRQFVTAGWGATSLDGAKISVRPIRNITVQAFGGSAIDPLNPQKVR
                     PLARNEIWGGYLAWDWRFKAHLGFSLFQKNFERSKDRYLIGADGRYDFGRVSFLARAN
                     YDYDRTSALFQRFFPLRLKKLQLLSLESRQTINPQFTTGLEYFFHRPLFSARSFMTRF
                     SQSTYHDWRTSGNYFLTPKIELHGEMGWALLEKEEGAPGSDNAFRLRLGVAASDFAFG
                     YVRRSGYAGSSDGVYARFAHQLRRWLLLSGSFDFSQYAIQPAEDETQATSAALSVDFR
                     PWPDWSLLVQYQNLTNVRFSNDNRLFARAAYNFRF"
     gene            complement(13946..14263)
                     /locus_tag="AC16FU_06510"
     CDS             complement(13946..14263)
                     /locus_tag="AC16FU_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR2994058.1"
                     /translation="MDGAEVLAKVAHLPISTWNYKSQPTSIRHIGPMAQDFYALFGVG
                     EDDKIISTIDPPGIALAAIQELYRENETLNARVSELESLVQTLLAGRQDKAAGDYHFG
                     SAR"
     gene            complement(14267..15232)
                     /locus_tag="AC16FU_06515"
     CDS             complement(14267..15232)
                     /locus_tag="AC16FU_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994059.1"
                     /translation="MLLTTALCLVATMAGVVRPGSPQAAVPQLINYQGKLTNASGQPI
                     TSAVTLKFRIYDTPTGGTLLWGPESQPVTPDANGVYNVILGQGTPPVPFPANLFAATP
                     RYIGVEVNSEGEMTPRQQITAVAYSLRVATVDGATGGTISGDVSIQSDLSVSGKATIG
                     PNGNSGGFAFVAGASNNVTNIGATVGGGQSNNASIDFATIDGGIGNNASGENSTVGGG
                     RFNTAAGSNSTVPGGASNRADGDYSFAAGRRARAFHTGTFVWADSNDFQFASEGPNQF
                     SARTTGGARFVTATDGAGNPTAGVFLFGGANSWSSISDRNAKTNF"
     gene            complement(15587..16369)
                     /gene="lepB"
                     /locus_tag="AC16FU_06520"
     CDS             complement(15587..16369)
                     /gene="lepB"
                     /locus_tag="AC16FU_06520"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2994060.1"
                     /translation="MNGKKDQAKPSASGGKLKKSFDQVLWEYVKSLAIALILALGIKT
                     SLVEAYKIPSGSMEDTLLVGDFLLANKFLYGSKIPLVNAHLPAIRDPKPGDVVIFKYP
                     VDRKTNYIKRCIAVGGQTVEIRNKKVYVDGKEYPFPAHGKFTDTMIMPQAGGGAAPFN
                     PYDPHNPLNRDNFGPYRVPEGMFFMMGDNRDNSFDSRFWGPVPRNLILGKAMVIHWSW
                     RRDERAPVWSWTNPFSMAKAFGYNLVHFFQRVRWNRLGSLVR"
     gene            complement(16503..17564)
                     /gene="rseP"
                     /locus_tag="AC16FU_06525"
     CDS             complement(16503..17564)
                     /gene="rseP"
                     /locus_tag="AC16FU_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR2994061.1"
                     /translation="MTTLLATIFVLGLLVFVHELGHFLVAKRAGIKVERFSLGFPPKM
                     IGKKIGETEYCISWLPLGGYVKMAGEEPDAAQVTGAPYEFMSKPIWIRSLVVVAGPVM
                     NYLLAVLLIWGIFFLGGRTEVDENQIVVGTVMPGSPAEKAGIKPGDKIMAVDGQAVKT
                     FDEMREKIYAKVEKPVEVTWLRDGREMRATIVTFKDKSRNEKGQPVAVGRIGIGQAFR
                     LYKVGFFEAARLGVVTSWDMAALVGQFLYRLIIGQESFKSLGGPVFIAQIAGESAHRG
                     FADLLMFAALLSVNLALLNILPIPALDGGHLLFLFIEKLRGKPLSLKQRAVIQQVGFA
                     FLILLIVFATYNDILRIFH"
     gene            complement(17561..18727)
                     /locus_tag="AC16FU_06530"
     CDS             complement(17561..18727)
                     /locus_tag="AC16FU_06530"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR2994062.1"
                     /translation="MKTIVVLGSTGSIGQNALLVCRRFPERLRVVGLSAYNNGALLAT
                     QARLFQPEWVAIVDKKQARTVKDDLPTGTRLLVGEEGIVELAQMPEADLVLNAIVGSA
                     GLLPSLAVLAAGKTLALANKESLVMAGELLMVQARVNNVEIFPIDSEHSAIKQCLFAG
                     RHPEVRRIILTASGGPFREKSRDEFAQVTVNQALAHPTWNMGKKVTIDSATLMNKGLE
                     IIEAHHLFGMPQEKIEVAIHPQSLVHSLVEFVDGSVLAQISPPDMKIPLQYALLYPER
                     LSSENGLLDLTRTGTLEFSEPDLTKFPSLLLARRALTLGGTAPAVLNAADEVAVQAFL
                     DEKIGFGEIPQVVRAVLDEHTVIDDPTLDDILKADRWAKSKAGEFVGITEEAAR"
     gene            complement(19726..20328)
                     /locus_tag="AC16FU_06535"
     CDS             complement(19726..20328)
                     /locus_tag="AC16FU_06535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2994063.1"
                     /translation="MLKVLVVLTFPLLVLGSPNKEVKKPVTDKKPQAATQAKYPQVVI
                     ETDFGNITVELYPDSAPKTVENFLKLEEKKFYDGLTFHRVEPGFVIQGGDPKGDGTGN
                     AGYSIPAEFNGRKHLEGTVAMARANDPNSASCQFYICLAPQPKLDTLGYTVFGQVISG
                     MDVVKKINQVPVTWTGAFNRPVKPVYMKRVSLKKSVSPPK"
     gene            complement(20446..21006)
                     /locus_tag="AC16FU_06540"
     CDS             complement(20446..21006)
                     /locus_tag="AC16FU_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994064.1"
                     /translation="MTGLAALWLPILLSSVIVFLLSWVIHMLPLWHKNDYPKMSNEDA
                     VRDAIRPLAIPPGEYIVPRAASAKDMKNPEFIEKLKKGPVLMLTVWRNEPFSMGKSLV
                     LWFIYCVVVGIFSAYVAGRALPSGTEYLHVFRFAGVTAFTAYAVALWQNSIWYKRSCS
                     VTAKLTLDGLIYALLTAGTFGWLWPR"
     gene            complement(21183..21404)
                     /locus_tag="AC16FU_06545"
     CDS             complement(21183..21404)
                     /locus_tag="AC16FU_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994065.1"
                     /translation="MKKFMAAVFVLTVALAFLGGLLIADGQAKPPKPPPPPPPQPCDH
                     VCFEFNGCICCQDCCPGQVCTDVVCVCTA"
     gene            complement(21539..21727)
                     /locus_tag="AC16FU_06550"
     CDS             complement(21539..21727)
                     /locus_tag="AC16FU_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994066.1"
                     /translation="MAMVVVLTVVLAFLGGLFIAESQAKPPKPCQRQCVTDEFGCFCC
                     RDCCPGQICPAFVCVCPL"
     gene            complement(21942..22127)
                     /locus_tag="AC16FU_06555"
     CDS             complement(21942..22127)
                     /locus_tag="AC16FU_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994067.1"
                     /translation="MKKLMAIVVLLTVALAFCGGLFISQSQADPCKNFCTTINGCTCC
                     QHCCGKICTQLPCWCIL"
     gene            complement(22207..>23126)
                     /locus_tag="AC16FU_06560"
     CDS             complement(22207..>23126)
                     /locus_tag="AC16FU_06560"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR2994068.1"
                     /translation="NTKQMSEGELERLSRGYIDHVAPFLGPDKDIPAPDVYTTPQIMA
                     WMMDEFSILRGSYAPGVITGKPLSVGGSAGREDATARGAVIVLREAGKAFEIDLKKST
                     LAIQGFGNAGYFLAKLAPEILGVKVVAVSDTHGGIYRLEGLAADAVEAHREKTGSVIG
                     FPGAQEISNAKLLELPVTILCPAAIEDQITSQNASKIQASIVAELANGPTTPEADEIL
                     EKNGVKVIPDFLCNAGGVTVSYFESVQNNYNYYWDEEAVHRKLDKKMTKAFADTFAAS
                     QQYRVSLRMGAYCVAVSRVVEAMQARGWI"
BASE COUNT         4897 a         6768 c         6461 g         5000 t
ORIGIN      
        1 gcggccagga agttgccgat ggcgtggtag ctgccgcgca ggctcaaggt gaacgccgcg
       61 gtgttgtaga acccggcggg actggtcccc tgcggggtca cctccaccac ccgcacctgg
      121 agccccgcgc cggccgagtt taactgctgg agcagcgcgg gcacctgctt ctcttccggc
      181 aaaaggagtt caatcgactg gtagcgaccc aagaggtcct ggtactcggt ctttagccct
      241 tcgaagcttt tggctttcaa ttccaccgct ttcaggtcgg ccatcagccg ctcgtactcg
      301 gcccgcttta ccttgagcgc cgccgcggtc ttgtcgtaga tgcgcacgta ccagacgtac
      361 cccaggaaga acagccccag gatcgccagg atcatcttct ggttttttgg atcgcggaag
      421 tccattgcgc gtctctccct tactcccgaa cgctgggtgc gcccgcctta cggcttgacc
      481 gccgaaatgg gtgccggctc caccggttcc accggctggc tctggaccag ctccagatcg
      541 caggagagct ggaagttgta ggccaggtgg ccttccacct cctgctcccg ggcgtactcc
      601 agctcgacat tcttaaagaa attcgatttc ttcatctgga tcaggaacgt ggcgatgctg
      661 ttgagcgagt aggcgaatcc ttccattttg gtccgctggg gggtggtcga acccgccgac
      721 gcacccgggg acggcgctcc ggccggcgct gtgggcggag cattcccgcc cggggcggcc
      781 gccgccggct gggccaggcc ctctttgaac gtggtgagcc acaggtactc cggcacccgc
      841 cgcgccaagt cttcgaacat gctcacgtac accgcccggt tgcggtccag ttgttcgatg
      901 gccgacagcc ggtctaaaat tttggctttg acttcctcca gggcatccac cacctgaatg
      961 tcgcggcgca tctgttccac ctgcgtgcgg gttttctgaa tccgcgtctg ccacttcttc
     1021 atctggtacc gttgcatcac cgtggccccg accacagcta ccaggagcgc cccgaccccc
     1081 accgagacgt accaggcggt gcggggcagg cggatgacgt gggaccgctt ccggagatct
     1141 ttggggagca ggtttatctc gatcatggct acttcaccct tcgggccgcg aggccaatgg
     1201 gcaccatcaa aagcggcgcg atgcgttcca agtccagatg ccggaactgg tccggatcgt
     1261 agtcgatgtt gcggagcgga ttggcaattt ccacgggggt ggcgaatttc gactgcatga
     1321 actccggcag aaacggcacc agggcgccgc cgccggacag catgatccac tccaccgccg
     1381 gcactttggc cgaggatttg aagtaggaga aggccacttc caggccggag aggagctctt
     1441 cggccgcgga aatgaccgtc acccgcagca tgtcggcatc cacggtgcct tccagttcgc
     1501 cgcggatcgc cttggcggtg agttcgtggt tcaagcggaa ttctttctga atcgcatcgt
     1561 aaatgatccg tcccccggcc gacacttcgc gggtggagtg gtactggccg tctttgagga
     1621 aggtgatgtt ggtggcgtcg aagccgatgt tcaccagcgc ggtcaccttt tgtgggtcga
     1681 tttcatagtt cgcctcgtag gcgttgagaa tggcgaaggc gtcgatatcg accaccaccg
     1741 cccggagccc ggcgtcggcg atgacgtcca aaaacgaccg gaggtactcg ttgcgggcgg
     1801 ccactaggag gacatccatc ttattggtct cttcattgac cgcaatgacg tggtagtcca
     1861 gggtcacgtc ctccacgtcg aagggcgccc gctgttcggc ttcgaagaga atcgcttgtt
     1921 cggcttcgct acccgatttt ttgtcgatgg agatcttatc ggtgatgacg ccgtgcccgg
     1981 cgagcgacac caccacctct ttggtgtgcg ggtcgcactg atcgaccaga ctctggatgt
     2041 tgaaaatcac cgtgtcgcgg tctttaatct cgtccgcgac gatggcctcc agtggcagtt
     2101 cgcgcacgcc cagggacttc aggctgtacc ccccggactt cttctccagc tgcaccagct
     2161 ttatggcgcc ggagccgatg tccagcgcgg cgacgttctg gcttctgcgt aagagatcgg
     2221 tgatcacgtg tcttcagtcc gttttgggcc ggcgctccgg ggccaccgct tttccctctc
     2281 cggtgctgcc ccacccgctg gcggttaacc tgctttccac tttcagatta tcgacccccc
     2341 tgccaaaata ttgagcaccc ccaaaaactt tttttgctcc ccacccaatg gggacccctc
     2401 cccgtctgcc aggggcggcg gcgggagacg gtacccaggg cacccatgga cgaaacccaa
     2461 aaacgtcccg gaaatcgggg acgtttttgg gtggtccggc ggacgaactg cgttaaaggg
     2521 cggtcaagcg tacccggccg gtggaggcca aaacgtcaac agcatagcgc caggtgccgt
     2581 cattggtctg gacgcggacg gcgccggagc ccgtggcctg gccggtggaa gtaaaaacga
     2641 cggtgttgtt ggtcaaggtg ctgttggaga gcgtcacgtc ggtcgacaga acctctcgcg
     2701 tgagtaagga atcgcccgtg tcgtaggttc ccgaactgtt gtcgtcccgg aagtaggtga
     2761 tccgccggtt atcaagctcg aagaggatgc cgtaccggat tttcttactc accgcctggg
     2821 agcgggccaa gcgcagaacc gagaccgcat cccgggcggc ccccttggcc ttgagctggg
     2881 tcatggtccg aaagaaccgc ggcgcggcca gggcggccaa caccccgacc acggccaccg
     2941 ccacgatcaa ttcgacgaac gtgaacccgc gatttttcct cagttttcgc atagcaccct
     3001 ctcgtttcct ttacagttcc cgccaggagg cgaaccgctt cttccaggtg taggtcgtta
     3061 ccgtacccgc accgggcatg ccgggcggga ggttgtcctt gagcgacggt acctggtaga
     3121 agtgcggcac gttggtgatg ttgaagtagt tggaaaccat ggccccagcc agttcgttct
     3181 gcttcgaatt gttcatctga tgctgggcga agaaggcccc ggtcagctct aagtgcgaag
     3241 cgcccgaccc ggcgatggtc atatcatgcc cggcgactaa cccgatgacg tcgtcggtcg
     3301 ggaaagcgcc cttcgagagc aggttattcg tgatcgaaat atcgttcctg gccgccagcg
     3361 tgccgcggcc gtcgtagatg atcggattat tcccgctgcc ccccttaccc tgctggatcg
     3421 ttatgttgcc ggtcacgtaa acgatgccgg aaatattcag gtgaccctgg tcatccagct
     3481 cgatgctgct tccgttgggg ccggttatct tcgccatcgc cgttcccgcc tccaggatga
     3541 ggtcactccc aatcaccagc gcatgggcca ggaggtaagc cgtgtagttg gcgtaggtaa
     3601 ctccgccgtc ggtatacggg tcgttcagat tgggaaagct taactgaccg ccgcctaagt
     3661 cgtacttttc gctggtgccg ttgtccgcgt aaacgttggt gggattcgaa ccgccaaagc
     3721 cgtcattgac gtagaccccg tccatcgttt ctttcaccgg gggtgagccg ccgctcacgt
     3781 ccggctctcc gatttgcgac gcgttggagt tgatggtcac gaggccgtgc tggacccgga
     3841 attcggcgtc cagggaggaa acgttttcgc cgccgaaggg aacctgcccc agagccggta
     3901 cgcgcgagga gagcgagacc ggcattccat tgtagttatt gtacaccccg gctccaccac
     3961 cgatatctaa tgccacgtcg gaagaagtta aaggggcggt gtagacgccg tcgccattgt
     4021 catcggtgta cggttcgagc ggatcatgga taccgttccc gttggtgtcg gtgaagcggt
     4081 cgccgttccc ctgaatgtga actgagccgt ggaaccccac gttgccgttg atcgccttgc
     4141 cgctctgccc cacgccggca aagagcacgt tgttccagac acttaaaggg ttgagtttgt
     4201 actgcacatc gacggcctgg gcgctggtgg cgtccgtgtt ccggccggag gaaatcaagg
     4261 tccggaccgg caggtcgttc cccatcgtca ccgtgaaggt cccgcgacca agggtattgc
     4321 tggcgttaat catgttcatc agctgggcgc tgtccagaat ggtgacgtta ttctgcatcg
     4381 tcttcagccc gtactccagt cccgcctcag ccgtatagaa ggcctcggtt ttcttctggt
     4441 agttgtggga aatgtcgcta tcggtcagcg aggtggtgac ggcggcaatg ccgatgaagg
     4501 ccagcatcac cagcaccacc atggcgacga gcatcgccgc tccggtttgg ttcatcagga
     4561 ctttacgcat acgtctcgct cctttgttct ctcttttcgt catcgattct ctgggccatt
     4621 gctgtcgttt ttcaaagtgc ctgctccgct accggttctt gagcttgacg ctggagacca
     4681 gccggcgggt gtaattcacg tactggttgc ccttgagcat ctgctcccgg gccaccagcg
     4741 tgaccgtgag ggtgttggtg ccaccgggca caaagcccac ggtgtcgacg tactccgcga
     4801 acacctgact ttccagactg tttatcttgc gcatgagccg gggattattt gcgatggagc
     4861 ggtccacgaa aaagcgcgtg gtatcggtcg ccgctccatt ctgggcataa atcgccagcg
     4921 tatcctggtt ggtattaacc tggaacgccg gcgcaccgaa gagcacgccg tagccggcgc
     4981 tgcggatggt gccggtcagt gcgtccatgc tgttgcggcc gttctgctgc agatcggcca
     5041 gctgggtctg ggtcagccac tggttgtgct gggtcaggta gaactccagg gtgaagccgg
     5101 ccaaaagcgc ggtcaagaac ccgacaatca acatttcaat aatcgagaaa ccgcgctggc
     5161 ttctgtaaat ctccgtccac ttctgcgccc agtggaatag ttttggtttc ttcattggct
     5221 tgtctcctcg tctggccagc accggattgc aaccatcatg ccggcgaata ctctttcctg
     5281 gctttctgcc taagttgaaa tcagcataga agaaaggaac ctgtccccag ggcaaatctg
     5341 gttcgctccc cagcggtcgg cgccaacgag cgggggttct ccccctactc tcggccggga
     5401 gtccggcggc cgttccatta gtattccgct tctggtttgg gacggtcacc ctgctcacgt
     5461 gcacgctctc tttcctgctc gtttttgtgc cgctcttgcg catcgttttc ctcacgaact
     5521 gaaagatcgt ctttttgctc cttacacccg ctcgcccgtt cggaagcgcg tcaccatctg
     5581 gaagaagttg ggccggaagc ccggcagggg tgccgctttt ttcaccagcc gccccgcgtg
     5641 gggtggccac gaccggacgg aatcaagttt ctgggagcgt tctcccggtt tgtcttgcat
     5701 ttctctgttc tcggtaatag tcatttccgt tcgccaactc gtcttttctc ccctgcccaa
     5761 ggtggccagg aagctttcac ttaagatcca gatcactccc accacccagc gcatggcgca
     5821 cccttacgac accggcttat aggtgttaaa gaccacccgc cgggtcacgt tgttctgttc
     5881 ggtccaggtg atggtgaccg cgatgcggta catgccggcc gggcaggttc cgtcggtggc
     5941 gttgttgtcg atccgcacgg tgcgcgtcag cccgccaccc ggattggtgg tctcggtgta
     6001 cggcatgggc gtgaaactgg cctggctttt taactgctcg atccggtcct gggcaaaaac
     6061 cgacgcatcg gtcacgtcct gggaatagga gttggtccgg atcgagagca cgatgagcgg
     6121 tgccagaccc aaaatgccga tggccagtac caccattgcc gagagcactt cgaccagtga
     6181 aaacccgcgt ttgttcttta atcttagcat tcgcactcct ccatcgtaat ccctattctg
     6241 ttctggctca cgctctgacc aattgcaaat ggattgccgt attcccggtt attgcacgaa
     6301 ccgacccggc cagcaacaac ccctttccct caaaagggtt accggtgccg accagagcgg
     6361 acaaaccgac aggtcagctc ttgcccatca aattttgagt tgacgcgcaa taaagtgtgg
     6421 ggaaaatccc acagcgagcg tggactaaaa ctcgatcgcg tacttcttca ttttgtcgaa
     6481 gagcgtcgtg tagtcgatct ggagaatttc agcggttttg cgcttgttgc cgcgggtctg
     6541 gttgagcacc cgctggatta aggcccgctc cgcctccatc tgcgcgtgcg cccccacttc
     6601 tttgagcccc gccccctccc gcagccggat ctcctgcacc gagggaatcc ggatcgccag
     6661 gtgctcgggc gtgatccgtt tcccttcgca gaggatcacc gcccgctcga tggtgttttc
     6721 cagttcgcgg acgttccccg gccagtggta gcggtccaga accgagaggg tctccgacga
     6781 gagggttttc ttgggctttt tcatttcggc gcagtatttg gcgatgaagt gctcggccag
     6841 aagggggatg tcctcgcgcc gttcacgcaa agggggaacg gcgagcggga aaaccgacag
     6901 gcgatagaac aggtcttcgc ggaactgctt cttatcgatg gcggctttga gatcggcatt
     6961 ggaggcggcg atgacccgca cgtccacgga caaagtcttg ttcccgccca gccgctcgaa
     7021 ggttttctcc tggaggacgc ggaggagttt cgcctgcagg gcgacgctca tatcccccac
     7081 ttcgtccaag aaaattgtcc cgccttcggc gatttcaaac ttccccattt tgcgcgccca
     7141 ggccccggtg aaggcccctt tctctgaacc gaagagttcg ttttccaaaa gttccgacgg
     7201 gatcgccgcg cagttgatgg ccacgtaggg tgcatttttg cggagcgaga ggttgtggat
     7261 ggcccgggcg aagagttcct tgcccgtgcc gctctcgcct aaaagcagca cggtcgtatc
     7321 gctgggcgcc actttctgca ccaggtgcga gacttcgacc attttttccg acttgccgat
     7381 gatctccgca taccccagcg agtgggccag ttcttcccgc aggagaatgt tctcggccaa
     7441 aaggcggcga ttttccagag cccgccggat cagaacgccc aagtggtcgg tgtcgaacgg
     7501 cttggtcaga aaatcgtagg ccccctcttt catcgcctgc accgccgtct cgatggtgcc
     7561 gtaggcggtc atgacgatga ccgagaggtc ggggtcgatt tctttcaatt tgatcaacac
     7621 ctgcagcccg tccatgtcgg gcagcttcag gtcggtcaga acgacgtcga agcgtttctc
     7681 tctggcgcgc tcgattccct gcgggccgga gggcacggtt tccacttcaa acccatccgc
     7741 cacgagcgtc tgggagagca tgttgcgcat gctgtcttta tcgtctatga ccagtacggc
     7801 cggcatccag tatcctccgc aactatgaaa aaattttgat cgaatttcct gattgtatta
     7861 tcggcggatt tggcaatctg tgaagtgtgg ggcaaccgga aaggaggata gcgggcgttc
     7921 aataggggga attttcgttc ctatgatttg cgaaccatgc ttttttgagg tcccgcggac
     7981 ttccacccgg tgatggcatt gccccgggta cccccgaacc ttcttagaat aaaaaaggga
     8041 taaacatctt cgtctgtttc atatacctct gataggtggc accaaaaaaa cgtaagttct
     8101 ccgcttcctc catcttagcg gtcattgtga gaaagaaaat cgtaataaca gccaaagaaa
     8161 caccaaccca ggaaggatgt ttgaaaaaca ctccccaggc cagaaacagt aacgaactat
     8221 agatgggatg acgaatgtag cgataagctc ccaccgtcac caattcagtg gtcttttcta
     8281 taccaatcaa cgctgggtcg ttccgctggc tggccggttt ccccactaac cttagtaatc
     8341 gagcaccgtg aattaccaaa aaagtggaga ctattagcaa gacccaagaa gctatctgat
     8401 ggatactgaa cggtgcataa aaccaataat tgaggtttaa cagaatcata accaaaatcg
     8461 cttcccaggc gaaaaagcgg tagaagccgt gagagtgcgg atcacgtacg gatgcccgtg
     8521 acacccaggc aattcctaca gatgcaacta taaaaagaaa caccttgaat tggaaaggca
     8581 tgctggcaat ccgaatatag caaactattt atttttacgc ttggaagagt aagcaagatg
     8641 ccggccaacg gctgagccga catcaggtcg actgagcggc tatgtcgagc tgagtgagtg
     8701 gcccaccgct tcaggaacat gttctctttg gctggctgga atggccaggt caaagctcct
     8761 cgaatgaaac gtctcggtct gcgattattg aagggaaact ctttgaaaaa gccacggaac
     8821 ggattcgaac cgttgacctg cggtttacga aaccgctgct ctaccagctg agctaccgtg
     8881 gcgtgaaaag tcaaggtaaa gacatgagag gacctgtcaa gccccgtcac cggtagaccg
     8941 gctcgtccct gagccggtca aggagaccag aacttcacgg ctatatcatt ttcctccggt
     9001 cactcgtacc acacgaattc aaacagcaaa tacgacccga ccagcatcac cccgaggtag
     9061 gcgatgagca tccggatgta gtcccagccc tcggcgaagc tcccgccttc caaggcgatc
     9121 cgggtggcgg tgatggagac gatgatcaaa ggcagcacaa tcggaaatga cagcgcggcg
     9181 aagagcgccc ctttgaccga cgccttggcg atgatggcgg cgatgatcgt cgtggccgcc
     9241 accagcccgc aggtgcccag aaatgtgacg accagaaaga gcggcaggtt gcccaacgcc
     9301 ggattgaaga gcaaaaagaa taaaggcagg atgatgattt ccagacccaa aagcaaaagg
     9361 aaattgaaac ccaatttccc gaggaacacc gcctccggcg cagccgctaa tttgagcgta
     9421 ttcgccgtgc gggcctcttc ttctttgacg aatacctgcg ccagccccga catcgccgca
     9481 aagaaaatgg caatccacaa aagcgccgcc aggacatccg gctccaggcg aacttgaccg
     9541 atggcgaaac tctccgccac caccgtcacc aggccaaaca ggaggatggt attgagcgcg
     9601 tagcgggtac gcagttccga gcggaggtct ttcgccaaaa ccgcccgggc gttaccggcc
     9661 cagccggatg acctggtcgc catagcgggt ttcttccttt tcattggtgg caatcaccac
     9721 gatgccgcgc tctttttgcc gctggatgaa cgcgtgggcc atttgtttgc cggcatcatc
     9781 taaattcgcc gtcggctcgt cgagcaacag gagtgtcggt tccagcaagc gggcgtaagc
     9841 gtactggagt ctctgcttca tcccggagga gtaggccttc accagatcgc cgccgcgctc
     9901 ggccaggccg acttcctgta acgccatttc gagatgcgcc tggggatgtt tgaacccgcg
     9961 cagccgcgtg aggaaggcga gattttccag cgcagtcaat tcgtcgtaca actgcaaatc
    10021 gggagcgacc aacgccagat ggcggcggag ttcctctttg ggaatttcac ccgaatcaag
    10081 ggcgagcgtg atccggcctg cagagggccg cagcaacccg gcgataatgc gcagaagtgt
    10141 cgtcttgccc gaaccgttgg gcccggtgac cactaacaca gaaccgtgtg caacggtggt
    10201 tgagatattt tcgaaaacga cgcggaatcc gaaacttttg gaaagaccgg aaaaggaaag
    10261 ggtcaccata ccctacggtg cataccagtt acgaaggtcc gtggggggat tgcggtgaca
    10321 gtgagtattc gaccctccgc aggtcttggc cgccggatcc cagatgtacc ctcgaccgcc
    10381 actattaaag gcaatgtcgt ataggccatt aacgtgaagg gcaagtgtac gaatggaagg
    10441 aattgttggc ggaatggctt cagtaactac ttggtcatga catgttgcac aggagagatt
    10501 ggggcccaaa gggtcaacat gaaatccatg ttttcctccc agcgcggcgg gattggcccc
    10561 cgcatcgtgg caggagccgc acagggcctg atccttccgg tagaaatggg ttgtaacgtt
    10621 attcccaccc cggaaattcc cgtgacagta gacgttctgg caggcgagtg aatccaaaga
    10681 gaattgtgcc ggagggagtg tatcgatttg cgcccgcaga ctggcgaact ggcttaagac
    10741 cacatctccc cgcccgcccg aactgttggt gacgtgtccc ggggcgatga cgaattccgg
    10801 tttgacgtgg cagtaggcgc agtcgaatcc ggagaaaaac ttgcgcccca gcaccatcga
    10861 ccggtgcgca cccaccccgc gggtggaatc gacttctctc aaactgtcgt tggccacggt
    10921 tgctacggta tctcccctgc ccgccacgtt gacgggcggc gcgccggtcg tatttccccc
    10981 tccgccgccg tggcaggcga tgcactgccg gccattcctc tgagtattgt gacagccgac
    11041 gcaggaaatt cccgacgagc cgcctttgaa gtccgtgccg tgacagcttt ggcacgtagc
    11101 gagcgctgag acgtctgtaa gtttagggag cataaacttc gcgtgatccg ctaaccaacc
    11161 cgtaggatgc accgccggcg cgacggcatt cttcttcaat ttactgcaac cggagcaaaa
    11221 actaattgcc atcatgaaga caaaaaaagt tattgccccc accgcaaacc gccggggcga
    11281 tttgctcttc ttatgaagca gttgcttcgc cggcaagcta cccatggcag gcagcgcagt
    11341 tgggctggct ttgatcgtgg caggcggcgc agcgctccag atcccgccgg gcagccgccg
    11401 cgtggaggtt gcgggcgcgc acgtcgcccc ggttcaccca gagtggatcg gaatggtcta
    11461 aaggcatccg caattcctgc tggtggcagg cgacgcagaa cgactgcgtt tcgtggcagg
    11521 aagcgcacag attcgttttc cctttcgcct ccagcccgtg cgtgaatttg aaattgagcg
    11581 ggtgaatcga gccggtatag ttatcgcccc ggtggcagct ctggcagagg tcctggccgc
    11641 ggtggcagct ggcgcagtag tcaccatcta agtttgcctt gaatttgtgt atctgtatcc
    11701 agttcgccgg atggagcacc gccatcatct gctgcggctc cgagtggcag gtggcacagg
    11761 cgttcgagag tttctcgccg gtatggcagg acaggcaaac cgccatctcc ggcatattgg
    11821 ccgctgtgag gacgaccgtc cgctccacat tggcatggca ggtctggcac gccgtcttct
    11881 gctcgatgtg ctttttgtgg gagaagatga tttcgcgttt gatcgccaga taagtcaccg
    11941 gcttgtcggg attgatgtgg caagtcttgc actccttggc ggccacgggc ccgttgtggc
    12001 aggagagaca aaccgcctct ttgggattga gccgatcggc cgccgcttcg ctcgtctgca
    12061 ccggttcgtg gcaggcaagg cacttgagtc ccacacgctg cagatgtttt ttgtggtcga
    12121 aaatgattgg cgatttagtc gctcccagcg ccccgaacga ctgtaggtag accacggcca
    12181 cactgcttcc caggccgatc agcaaaaatg aaccaagaat tcttttcatt gtcggtcaga
    12241 agcgaaagtt gtaagcggcc cgagcaaaga ggcggttgtc attggagaag cgcacgttgg
    12301 tcaggttttg atattgcacg agaagcgacc agtcgggcca gggccggaaa tcgacggaga
    12361 gggcggcgct cgtggcctgg gtttcgtctt ctgccggctg aatggcgtac tgggagaaat
    12421 caaagctccc ggatagcaga agccaccgtc gcagctgatg tgcaaagcgc gcatagaccc
    12481 cgtcgctcga ccccgcgtac ccgctccgcc ggacgtagcc gaaggcgaaa tcggaagccg
    12541 ccacccccag gcgcagccgg aaggcattgt cgctgccggg ggcaccttcc tctttttcga
    12601 gcagcgccca gcccatttca ccatgaagtt caattttcgg tgtcaaaaag taattgccgc
    12661 tggtgcgcca gtcgtggtag gtgctctggg agaagcgggt cataaacgag cgggcggaaa
    12721 agagaggccg gtggaagaag tattccaagc cggtggtgaa ttgggggttg atggtctgcc
    12781 ggctttccag cgagagcaat tggagtttct tcaggcgcag cggaaagaag cgctgaaaga
    12841 gcgcgctcgt ccggtcgtaa tcgtagttcg cccgcgccag aaaactgacc cggccaaagt
    12901 catagcgtcc gtcggcaccg atgaggtagc gatcttttga ccgctcgaaa tttttctgga
    12961 aaagcgagaa accgaggtgc gctttgaagc gccagtccca cgccaggtac ccgccccaga
    13021 tttcgttgcg cgccagcggc cgcacctttt gagggttgag cgggtcgatg gccgagccac
    13081 cgaacgcctg cacggtaatg tttcgaatcg gtcgaacgct gattttggcc ccgtctaagg
    13141 acgtggctcc ccagcctgca gtgacaaact gtcggcccac ccgcgctgcg agtttctgcg
    13201 acttcgtccg gtaatcgaag tagagattgt agaagttcca ctggggatcc cccggtaagg
    13261 cgttctgcca gtcggtgcgg aagcgcaaaa acatatggcc ggacaatagc ccgccccggc
    13321 cggaggttaa atcattggcg aaaagggtga atgtctgatt accacgcgat tggggaattt
    13381 cttctgtaga agtaatccgc tcgaatgagt agaccgaggt ggcgagcgtt ccggaaaagt
    13441 cggcggcggg ggcgactgcc ccacgggtga ggacgagcaa acttccccag aaaagagcgc
    13501 gcaaaaaatt ctgtcgcatc tgttggtgca accgtgttcg atacgaactc gtaccgagcc
    13561 aagctacgtc cggttcccgt gagtgtcaag aaaaacgaag gggtggacat ttacctccac
    13621 ccgaatgaat ctcagacttc ggtcaggcag gatgcctgac ctatggaagg gaaggtgttt
    13681 ttacttccat agccgaggct ttcagcctcg gaaggcactc tggccctaca gcccaggggc
    13741 ttttccccgt aacctggtag accggctcgt cctcgataga gtcgcaccga cccgagctgg
    13801 tcaaaaaata aaagtcggac ttcagagacg agcccgacta ccagtattgc gtgtcaagaa
    13861 aattgtaggg gcggacgttt acgtccgccc gaacagttct tttcgggcga acctaaaggt
    13921 acgcccctac gccactgtcg tcaggtcacc gggccgaacc aaaatggtaa tcaccagcgg
    13981 ccttgtcctg ccttccagcc aagagtgtct gaacgagact ctccaattcg ctcacccgcg
    14041 cgttgagcgt ctcattctcc ctgtaaagtt cctgaatggc ggccagggca atccccggcg
    14101 ggtcgatggt ggaaataatc ttatcatctt cacccacgcc gaacagcgcg tagaaatcct
    14161 gcgccatcgg accgatgtga cggatggagg tgggctgtga tttgtaattc caagtactga
    14221 taggcaagtg ggccaccttt gccaaaacct ccgccccatc cactggctag aaattcgttt
    14281 ttgcgtttcg atcgctaatg gacgaccaag agttagcacc accgaataga aaaactccag
    14341 ctgttgggtt gccggctccg tcagtagctg ttacaaatcg agcgccaccg gtagtgcgag
    14401 cgctaaattg attcgggcct tcagatgcaa attgaaaatc gttactgtcg gcccacacga
    14461 aggtaccggt gtggaacgct cgcgcacgcc gtccagccgc aaaagagtaa tcgccgtctg
    14521 ctctgtttga agcgccacca ggaactgttg aattacttcc ggcagcggtg ttgaagcgtc
    14581 ctccgccaac tgttgaattc tctccggatg cgttgttgcc aattccacca tctattgtcg
    14641 cgaagtctat agatgcgtta ttgctttggc cacctccgac agttgctcca atattggtga
    14701 cgttgttact cgccccagct acaaaagcga aaccgccgct gttgccatta gggccaatag
    14761 tggctttacc ggagacagag aggtcgctct ggatgctcac atcgccggaa atcgttccgc
    14821 cggtcgcgcc atcgacggtg gcaactcgta atgagtaggc cacggcggtg atctgctggc
    14881 ggggagtcat ttccccctcg gagttcacct ccacgccaat gtagcgtgga gtggccgcga
    14941 acaagttggc gggaaagggc actggcggcg tcccctgccc gagaatgacg ttgtagacgc
    15001 cgttggcatc gggtgtcacc ggctgcgatt cggggcccca caagagtgtc ccgcccgtcg
    15061 gagtgtcgta gatgcggaac ttcaacgtca ccgccgaggt aatcggctgc ccggaagcgt
    15121 tggtcagttt cccctggtag ttgatgagtt gcggcaccgc agcttgtggt gagccaggtc
    15181 gaaccacgcc ggccattgtt gccacaaggc acagcgccgt tgtcaagagc aaagttcgca
    15241 tgcgcacggt aaacctcctt ctggagttgc ttgcgaccca gtggtttgga aaatgtgaac
    15301 ggtgtgaaac gaatcgcaac atgaattccc cattacgaca cctcgcggtc accggttcat
    15361 cacctactaa cagaacgccc cccctcgctg aaaaagatat ggggtgaact gaacctgtca
    15421 aggattttta gagtttttta ggagttatat ggtcttgtat cgcctgggca ttgaagagtt
    15481 gttctccgtg cctcctcacc gttggggatc ttcaactgcg gtacgcattc tctacgcaaa
    15541 ataaaaaccc cgcttttggc ggggctgtct gcagagagaa aatcgcttag cgaaccagcg
    15601 accccagccg gttccagcgc acccgctgga agaaatggac gaggttgtag ccgaaggcct
    15661 tggccattga aaacggattg gtccacgacc agacgggcgc ccgttcatcc cgccgccacg
    15721 accagtggat caccatcgcc ttgcctaaaa tcaagttgcg cggcaccggc ccccaaaagc
    15781 gggagtcaaa cgagttgtcc cggttgtccc ccatcataaa gaacatccct tccggcaccc
    15841 ggtaggggcc gaagttgtcc cggttgagcg ggttgtgcgg atcgtagggg ttgaagggcg
    15901 ccgccccacc gcccgcctgc ggcataatca tggtatcggt gaacttgccg tgcgccggga
    15961 acgggtactc cttgccgtca acgtacactt tcttatttcg gatctccacc gtctggcccc
    16021 ccaccgcaat gcagcgcttg atgtagttgg tcttccggtc caccgggtac ttgaaaatca
    16081 ccacatcccc cggcttgggg tcgcggatgg ccggcaggtg ggcgttgacc agaggaatct
    16141 tcgagccgta gaggaatttg ttcgccagaa ggaagtcgcc gactaaaagc gtgtcttcca
    16201 ttgagccgga gggaatcttg tacgcctcca ccagcgaggt tttgatcccc agggctaaga
    16261 ttaaggcaat ggctaacgac tttacgtatt cccacaggac ctgatcgaac gatttcttta
    16321 gttttccgcc tgaggcggac ggtttagcct ggtctttctt cccgttcatt taccctccca
    16381 aagcatcttt catttacact ggtatacgta aggaagccgt ctctgaacta ataatacgtg
    16441 aaccggaacc tgtcaatcgc caaaaggaaa tgccgtttct ccggcggaaa ccgtcatccg
    16501 gttcagtgga agatgcgcag gatgtcgttg taagtggcaa agacgatcaa aaggattaaa
    16561 aaggcaaaac ccacctgctg gatgaccgcc cgctgcttga gcgagagcgg cttgccgcgc
    16621 aacttctcga tgaataagaa gaggagatgc ccgccgtcca aggccggaat gggcagaatg
    16681 ttcaaaagcg ctaagttgac cgacaggagc gcggcgaaca tcaaaaggtc ggcgaagccg
    16741 cggtgggccg attccccggc gatctgggcg atgaagaccg gcccgcccaa agatttgaac
    16801 gattcctggc cgatgatgag ccggtagaga aactgtccca ccagagccgc catatcccaa
    16861 ctggtgacca cccccagccg cgccgcctcg aagaagccga ctttgtacag gcggaaggcc
    16921 tgaccgatcc cgatccgccc gacggctacc ggctgacctt tttcgttgcg cgatttgtct
    16981 ttgaaagtga cgatggttgc gcgcatttcc cgcccgtccc gcagccaggt gacttcaacc
    17041 ggtttttcca ctttggcgta aatcttctcc cgcatttcgt cgaaagtttt gaccgcctgg
    17101 ccgtccacgg ccataatctt gtccccgggc ttgatccccg ccttttcagc gggactcccg
    17161 ggcataaccg tcccgacgac gatctggttt tcatcgactt cagtccggcc gccaaggaaa
    17221 aagatgcccc agattaaaag gaccgccaag aggtaattca tcaccggccc ggcgacgacc
    17281 acgagtgacc ggatccagat cggcttggac ataaactcgt acggcgcgcc ggtgacctga
    17341 gccgcatccg gctcctcccc cgccattttg acgtacccgc ccagaggcag ccaggagatg
    17401 cagtactcgg tctcgccgat tttcttgccg atcattttcg gggggaaacc cagagaaaaa
    17461 cgttcgactt tgatcccggc ccgcttggcc accaaaaagt ggcccagttc gtggacgaaa
    17521 accagaagcc ccaagacgaa tatggttgcc agtagtgtgg tcaccgcgct gcctcttccg
    17581 ttatgccgac gaactctcct gctttgctct tggcccaccg gtcggctttc agtatatcgt
    17641 ccaatgtagg gtcgtcgatg accgtgtgct catccagcac cgcccggaca acctgcggga
    17701 tttcgccaaa accgattttt tcgtccagaa aggcctgcac cgcgacttca tcagccgcat
    17761 tgagcaccgc cggcgcggtc ccgcccagag tcaaagccct gcgggccaaa agcaaactgg
    17821 gaaactttgt cagatcgggt tcgctgaatt ccagcgtgcc cgtccgggtc aaatccaaca
    17881 gcccgttttc cgacgacaac cgctccggat aaagcagcgc gtactggagc gggatcttca
    17941 tatcgggcgg gctgatctgt gccaaaaccg agccgtccac gaattccact aaagagtgga
    18001 cgagcgactg cgggtgaatg gccacttcga ttttctcctg cggcatgccg aacaggtggt
    18061 gcgcctcgat aatctccagc cctttgttca tcaaggtggc cgaatcgatg gtcaccttct
    18121 tccccatatt ccaggtgggg tgcgccaggg cctggtttac agtgacctga gcgaactcgt
    18181 ctctggattt ctcccggaac ggcccgcccg aggcagtgag gatgatccgc cgcacttccg
    18241 gatgccttcc ggcgaaaaga cactgcttga tggccgagtg ctcgctatcg atgggaaaaa
    18301 tttcgacgtt attgacccgc gcttggacca tcaagagttc cccggccatt acgagcgatt
    18361 ccttgttggc caaagcgagc gttttgccag cagcaagaac ggctaagcta ggcaaaagcc
    18421 ccgccgagcc gacgatggcg ttcaatacca gatcggcttc gggcatctgc gctaattcga
    18481 cgatcccctc ttccccgacc aacaagcgtg tgcccgtcgg caagtcatct ttgaccgttc
    18541 gcgcctgctt tttatcgaca atagccaccc actccggctg gaagagccgc gcttgcgtgg
    18601 ctaagagcgc cccgttgttg tacgccgaga gcccgacgac gcgcaagcgc tccggaaagc
    18661 gccggcaaac caaaagggca ttctggccga tggagccggt ggagccgagg acgacgatag
    18721 ttttcataaa cgagtcatga atatagccat ttgaacgctg aagaaaagag tccacatgat
    18781 ggcaggtatc taaagttcgt tacaagcctc gctttaaact aggaaacctc cataaggttt
    18841 aattttcaat ggctcgtaag agcttgatat gatctcggta tatatcacac taccaccacg
    18901 gatcaacccg ttccccttca aacaaagtct tcaaaaagtc ctggaggtcg tgtagtggta
    18961 aactaaatgc gacagaagcc cgatggtcct atactttcgg ttttacatga ccgctgttca
    19021 tttcacctgt tggctaacat actttaatgg caggcatcgt gaggaataat caccgcacca
    19081 ccgcacttac agcaaaccag tgcggctctg ataactaccc ccaacctatg gccttaaaag
    19141 aaattactgg gtagtctgct ggctagagtg gcagcaggtc gcatcacaga ttgatggaca
    19201 atcaaccgtc caacaatcga aacccgctga ccagccagaa acgtcagtgc aattggtgct
    19261 acagtggcag cagaaataat agccttgtcg agcctcatcg ggtgtacaag ttgctaacca
    19321 accataagcg ggacacccat agtaatgagg atctaaagaa acagccttaa tggtactcag
    19381 tgtcactaag aacgaaactc ctatagtggc aactactaat gctgccattt ggaatagccg
    19441 tcgcctcttc cgcataatac ctccacctct aggctaactt ttactcaata cttaacacca
    19501 ttcaattgcc taatttaaat atatacgaag aacctgtcaa gacttttttc actttttcac
    19561 cggcatgggg atttctaagg tgcttgacat tagttaaaat atttcattat caggaaattc
    19621 tcgaggctcg aaaaacttag atgttgcaca aaatgacgct aagtcctcga agggttgttc
    19681 ctgttcaaac ggcatctcga actacctatt gagaagaaga ttatcttact ttggaggtga
    19741 aactgacttt ttcaaggaca ctctcttcat gtagaccggc ttgacgggtc ggttgaatgc
    19801 tccggtccac gtcacgggca cttggttgat ctttttcacc acgtccatac cggaaataac
    19861 ctgaccgaag acggtatacc ccaacgtgtc cagcttcggt tgtggtgcca aacaaatgta
    19921 gaactggcaa gaggccgaat tggggtcgtt cgcacgcgcc attgccaccg tgccttcgag
    19981 gtgtttgcgg ccgttgaact cggcggggat agaatatcct gcgttgcccg taccgtcgcc
    20041 ctttggatcg ccaccctgaa tgacaaatcc cggttcgacc ctgtggaagg ttaaaccatc
    20101 gtaaaacttc ttctcctcca atttcaggaa attctcgacg gtcttcggcg ccgagtccgg
    20161 gtaaagttca acggtaatgt tcccgaaatc ggtttcgatg acaacctgcg ggtacttcgc
    20221 ttgggtagcg gcctgcggct ttttatcagt caccggcttc ttcacctctt tgttggggct
    20281 tcccagaacg agtaacggaa acgtcagtac caccagaact tttagcatgc acctctcctt
    20341 tttagttcat ttttcataac accgcgtcaa gatccgagcg ccgctgaagc ttgccacgcg
    20401 gtgctcgtca gcttctaagc gttgttgggc cgcgcaaacc ctgacctatc gcggccagag
    20461 ccatccgaac gtacccgccg tcaagagcgc gtagatcaag ccgtccaggg tgagtttggc
    20521 cgttacgctg caggaacgct tgtaccagat ggagttctgc cagagcgcca ccgcgtaggc
    20581 cgtaaacgcc gtgaccccgg caaagcggaa gacgtgcaga tactccgtgc ccgagggcag
    20641 cgcccgcccg gccacgtagg cggaaaaaat cccgaccacc acgcagtaaa taaaccagag
    20701 gaccaagctt ttccccatcg aaaagggctc gttgcgccag accgtaagca tcagaaccgg
    20761 gccttttttc agcttctcga tgaactccgg gtttttcata tccttggcgg aggcggcgcg
    20821 gggaacgata tattcccccg gggggatggc cagcgggcgg atggcatccc tgaccgcatc
    20881 ctcgttcgac attttggggt aatcgttttt atgccagagc ggcagcatat ggatgaccca
    20941 gctgagcaga aagacgatca ccgacgacaa aagaattgga agccacaacg cggccaaccc
    21001 ggtcataacg acctcctttg ctgagaccac tcgtttccta cgggcagact cctccgtcct
    21061 tcgtccatgc gcggcctaac ataacgaata ccgaacagtt gcgcaaaagt tttcccgaac
    21121 aaaaaaagca aaccctgggc cagataatca ccagggttcg ccctcgtccg tttcccggca
    21181 ggttacgccg tgcagacgca gaccacgtcg gtgcagacct ggcccggaca gcagtcctga
    21241 caacaaatgc agccgttgaa ctcgaagcag acatgatcac agggctgcgg cgggggtggc
    21301 ggcggcggtt tcggcggttt ggcctggcca tcagcaatca gaagcccgcc taaaaacgcc
    21361 agcgccacgg tcagtacgaa cacggcggcc atgaacttct tcacgtgcac tcacctcctc
    21421 tcttgttgtg agcgattcct caagtgaacg cctttcccgg ttcctccgct gggaatcata
    21481 aacggcagct ccccgggatg gctgccgcct gggtccgctt tcgtccgcgt ctgacttttt
    21541 acagcggaca aacgcagacg aacgccgggc agatctgacc ggggcagcag tcccggcaac
    21601 agaagcagcc gaattcatcc gttacgcact gccgctgaca aggtttcggc ggcttggctt
    21661 ggctctcagc gatgaacagc cccccgagga atgcgagcac cacggtcagc actacgacca
    21721 tggccatggc tttcttcatg cgaattcacc tccttccaat ggctgaacgc cccaaaaaag
    21781 gccttctgga caaaacgatt ccctgtaccc gacttctccc cgcaatgcct tcggctcaac
    21841 gcttttgctg cagaagtcgc accgatccgg ttcatcggct tccatgtctg caagaatgcg
    21901 aaccctggag caaccggcct ccagggttcg ctcgcgcccg cttacaggat gcaccagcag
    21961 gggagctggg tacagatctt tccgcagcag tgctggcagc aggtacagcc attaatcgtc
    22021 gtgcagaagt ttttacacgg atcggcctgg ctctggctga tgaagagccc cccgcagaac
    22081 gccagcgcca ctgtgagcag cacgacgatg gccatgagtt tcttcacact acctcacctc
    22141 cttccgtgga taatcgccgg ataaactctc ggagttcccc aaatcccaat tcctactatc
    22201 cggtctttag atccaccctc ttgcctgcat cgcctcaacc acccggctca ccgccacaca
    22261 gtacgcaccc atccgcagcg acacccggta ctgctggctc gcggcaaaag tatccgcaaa
    22321 ggccttggtc atttttttgt ctaatttgcg gtggacggcc tcttcgtccc agtagtagtt
    22381 gtagttgttc tgcaccgatt cgaagtagga gacggtcact ccgccggcgt tgcagaggaa
    22441 gtcggggatg accttcacgc cgtttttctc taagatttca tccgcttccg gcgtggtggg
    22501 gccgttggcc agctccgcca caatcgaggc ttgaattttg cttgcgttct ggctcgtgat
    22561 ctgatcctca atcgccgccg gacagagaat cgtcaccggt aattccagaa gcttcgcatt
    22621 cgagatttcc tgagcacccg gaaaaccaat gaccgagccg gttttctccc ggtgcgcctc
    22681 cactgcgtcg gccgcaagtc cctcgagccg gtaaataccg ccatgcgtgt cggagacggc
    22741 aacgacttta actcccagaa tctccggcgc caattttgct aaaaagtagc ccgcattacc
    22801 aaaaccctgg atcgccagag tcgatttttt caagtcgatt tcgaacgcct tccccgcctc
    22861 acgcaggacg atcaccgccc cgcgcgccgt ggcatcttcg cgtcccgctg acccgcccac
    22921 cgacagcggt ttgccggtaa tcaccccggg agcgtaactg ccgcgcaata tggagaattc
    22981 atccatcatc caggccataa tctgcggagt agtgtagaca tcgggcgcag ggatgtcttt
    23041 gtccggtccc agaaaaggag ccacatgatc aatgtagccc cgggaaagcc gttccaactc
    23101 accctcgctc atttgcttcg tattgc
//
LOCUS       DBNGZA010000300         1058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_482184, whole genome shotgun sequence.
ACCESSION   DBNGZA010000300 DBNGZA010000000
VERSION     DBNGZA010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_482184"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC16FU_06565"
     CDS             complement(<1..298)
                     /locus_tag="AC16FU_06565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913087.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HIR2994069.1"
                     /translation="MPTYDKVLFKYIDDPGQYRVDTYIKHGGYVALAKALKMAPDAVT
                     AEVKKSNLRGRGGAGFPTGLKWSFVPKKPGPKYLLCNADESEPGTFKDRELMEKD"
     gene            complement(476..1042)
                     /locus_tag="AC16FU_06570"
     CDS             complement(476..1042)
                     /locus_tag="AC16FU_06570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR2994070.1"
                     /translation="MAVATPEKKPGKTGPSPGIKLLLPQTIQKMRQALPKFPVRRSAI
                     LPALHAAYDQLGYLNDEIYAEISQVIGVPALEIAEAATFYTLFPKKKVGKYLIMVCTN
                     ISCALCGADSLVSYLEQKLGIKNGETTPDGLFTLWTVECLGSCGTAPMLQVNEAFYEN
                     LTREKVDRLLEAWQQGKPVEVKIMERGW"
BASE COUNT          200 a          319 c          282 g          257 t
ORIGIN      
        1 ggtctttctc catcaattcc cggtctttga aggtccccgg ctcgctctcg tcggcgttgc
       61 acaagaggta cttcggcccg ggcttcttcg gcacgaaact ccacttgagc ccggtgggaa
      121 accccgcccc cccccggccg cggagattcg atttcttcac ctcggcggtg actgcatccg
      181 gcgccatctt cagcgccttg gctaacgcga cgtaaccacc gtgcttgata taggtgtcaa
      241 cccggtattg ccccgggtcg tcgatgtatt tgaaaagaac tttgtcgtac gttggcatta
      301 tcgtaacggc ttcgttaccg caacgggagg cgcgacttta cctgtggtga gcgaagccga
      361 accagtcgcg cgctccccat tgctattttt tctccagaac cgggcttaag cccggttctg
      421 gagaaaaatt ccgggcgatg ctttcgggcg actgaagtcg cccctcccgg gtaaactacc
      481 atccccgttc cattatcttc acttccaccg gtttcccctg ctgccaagct tcgagcaggc
      541 gatcgacttt ctctcgcgtc agattttcgt agaacgcctc gttaacctgc aacatcggcg
      601 cggtgccgca ggagcccagg cactccactg tccagagtgt gaataggccg tccggtgtcg
      661 tctccccgtt tttgatgcct aacttctgtt cgaggtaaga aacgagacta tccgctccgc
      721 agagcgcgca ggaaatgttc gtgcagacca tgatcagata tttgccgact ttcttcttcg
      781 ggaagagggt gtagaaagtg gccgcttcgg caatttccaa agccggtacg ccgatgacct
      841 gactgatttc cgcataaatc tcatcattta agtagccgag ctgatcgtag gcggcatgga
      901 gtgccgggag aatcgctgac cgccgcaccg gaaatttcgg gagcgcctgc cgcatttttt
      961 gaatcgtctg cggcaagaga agtttgattc cgggcgaagg gcccgtcttt cccggctttt
     1021 tttcaggcgt cgcgacagcc atcagcggtc aatctccc
//
LOCUS       DBNGZA010000301         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_692826, whole genome shotgun sequence.
ACCESSION   DBNGZA010000301 DBNGZA010000000
VERSION     DBNGZA010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_692826"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="AC16FU_06575"
     CDS             <1..1219
                     /locus_tag="AC16FU_06575"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR2994071.1"
                     /translation="LAGTDLRTLVLTSATDPRLPAPMAEAEGATADDWEMIKSIGSIS
                     TPTLFVLTLPNPVVYWNQLTTELGRARGLPPPRFARGYALTQVAIYDALVAGRVITRR
                     GLADNALAAGAASEVSFYLFPLDSMHIKEAIANQVDLRHPDLRTLRSWFLGKLVGRLV
                     VIYGQHDGSDAVFPGPQPTGDCIWTGVNPVLPMCGTWKTWITTSGAEFQPEPPYPCGS
                     AQDLLEVEQVYDASLVRTPEQIAIVHKWADLPPPTIWNGYLNDRLISNDWSNLRSARA
                     HAFLNVTMYDAFVSCWRTKYITYTWVARPFQRLAGRVPAFTTVITTPNFPTYTSGHST
                     ISAAAAVVMGELFPGEQAFFNAEAEEAALSRFWGGIHFHHDDNQGLVVGRQIGEKTVR
                     IMRHLGIPVLAGE"
     gene            complement(1275..2432)
                     /locus_tag="AC16FU_06580"
     CDS             complement(1275..2432)
                     /locus_tag="AC16FU_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR2994072.1"
                     /translation="MNHLEFQNSPIGRMVLTPRGCWAFVPEKLPPPLEYDPALVFLLD
                     EASRALARLAGLGERLPNPRLLIAPYLRREAVLSSRIEGTQTSLSDLFLFEAARSQPA
                     NGSDVKEVLNYVLALEHGLRRIKELPLSLRLVREIHGKLLEGVRGQEKQPGSFRTIQN
                     YIGPPGCKEEEATYVPPPVTELHRLLDKWEKFLHDDSGIPPLIQCGILHYWFEAIHPF
                     FDGNGRVGRLLIILFLCAREHLSQPQLYLSAFFEKHRGEYYDRLLAFSRDGDWKNWLL
                     FFLRGVAEQSKDAVERMQRMLSLQEKYRNRLDEAKANANVLRLLDQLFRNPYTNIPRA
                     AKQLRVSYLTAQAAISFLGRQGIVQEVTGKKRYRIFCARELLRTIEVDLTT"
BASE COUNT          596 a          705 c          673 g          639 t
ORIGIN      
        1 gttagcggga accgatctgc gcacccttgt acttacctcg gccaccgacc cccgcctgcc
       61 ggcgccaatg gccgaagcgg aaggagctac cgccgacgat tgggagatga taaaaagcat
      121 cggctccatt tccaccccaa ccttatttgt ccttaccctc cccaatccag tagtttactg
      181 gaaccaattg accaccgaac tgggacgggc aagaggattg ccgccgcccc gttttgcccg
      241 cggatatgct ttgacccagg tagccatcta cgatgctctg gtagcgggac gtgttatcac
      301 ccgccgcggt ttggcggata acgccctggc ggccggtgct gcctcggaag tgtcgttcta
      361 tcttttcccg ctggattcga tgcacattaa agaagcgata gcaaaccaag tggacttgag
      421 gcatcccgac ctgcgaactc tgcggagttg gtttttgggt aaactggtag gcaggctggt
      481 cgtgatttat ggtcaacatg acggctcgga tgccgttttt cccggtcccc agccgaccgg
      541 cgattgcatc tggaccggag ttaatccggt tttgccgatg tgcggaacgt ggaaaacctg
      601 gatcaccact tccggtgccg aatttcagcc cgagccacct tatccctgcg ggtcggctca
      661 ggatcttctt gaggtggagc aggtgtacga cgcgtctctc gttcgcactc ccgagcagat
      721 cgccatcgtg cataaatggg ccgatctccc tccgccgacc atctggaacg gttatttgaa
      781 cgaccggctt atcagcaacg attggtcgaa tctacgttcg gcccgggcgc acgccttttt
      841 gaacgtgacg atgtatgatg cttttgtttc ctgctggagg accaaataca tcacctacac
      901 gtgggtggcg cggccgttcc agcgcttagc cggccgggtg cccgctttta cgacggtgat
      961 tacgaccccc aatttcccga cctacacttc cggccattcc accatctccg cggcggcggc
     1021 cgtcgtgatg ggtgaactct tccccggaga acaggctttc ttcaacgccg aagccgagga
     1081 agcggccctt tcccgcttct ggggcggcat tcacttccac cacgacgaca accagggtct
     1141 ggtggtggga cggcagatcg gcgagaagac ggttcggatc atgcgccacc tgggcatccc
     1201 cgtactggct ggtgaataga atttctgtga aaacttgaac gggtgtggga gcactcctgc
     1261 acccgttttt gtttctacgt cgtcagatcc acctcaatag tccgcagaag ttctcgggcg
     1321 cagaagatcc gataccgctt tttgccggtt acttcttgga caatcccttg acgcccgagg
     1381 aaactaatcg ctgcttgagc cgtgaggtac gacaccctta actgtttagc agctcgcgga
     1441 atgtttgtgt aaggattccg aaaaagttga tcgagaagac ggagcacatt ggcatttgct
     1501 tttgcttcat cgagacggtt acggtacttc tcttgaagcg aaagcattcg ttgcatccgc
     1561 tcaacggcat ccttagattg ctccgcaaca ccccgaagga aaaacaacag ccaattcttc
     1621 cagtcgccat cacggctaaa cgccaaaagt ctatcgtaat attctccccg gtgcttctca
     1681 aagaatgcac tcaagtaaag ctgaggttgt gatagatgct cacgtgcaca caaaaatagg
     1741 atgatgagga gccgcccgac acgaccgttc ccgtcgaaaa aaggatgaat cgcctcgaac
     1801 cagtaatgga ggattccaca ctgtatcagc ggcggtattc ccgaatcgtc gtggagaaac
     1861 ttctcccatt tatcgagaag tctgtgcaat tctgttacgg gtggaggaac gtaggtggcc
     1921 tcctcttcct tacatcccgg cggaccaata tagttttgaa tggtacgaaa ggaaccgggc
     1981 tgtttttctt gaccacgcac cccttccaaa agtttgccgt gaatttctct gacgagtcga
     2041 aggcttagcg gaagttcttt aattcgtctt agtccgtgtt caagcgccag tacgtagttt
     2101 agaacttcct tcacgtcgga accatttgcc ggttgactcc gggcagcttc aaataaaaag
     2161 agatcagaga gagaagtttg ggtaccttca atgcgagaac tcaataccgc ttcgcggcgt
     2221 agatacggag cgatgagcag acgtggattg ggcagtctct ctcccaaacc cgcgagtctc
     2281 gccagtgctc gactggcctc atccaacaaa aaaactaagg cgggatcata ctctaagggg
     2341 ggtggcaatt tttcgggtac gaacgcccag catccacgag gcgttagtac cattcttccg
     2401 ataggactgt tttgaaattc aaggtggttc attattcttg ttcaaaatgc ctaaaaattt
     2461 acttgttaca ataacctgtc ttatttaaga aaatagccct tttcttaaat aagagcaatg
     2521 gctatttagg tttccctaaa taaaaaagtg tgaaagagat tgcaggtctt ttaacgatcg
     2581 ggggtgcgta atcctcctct gtatctcttt ggg
//
LOCUS       DBNGZA010000302         8155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_482322, whole genome shotgun sequence.
ACCESSION   DBNGZA010000302 DBNGZA010000000
VERSION     DBNGZA010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_482322"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="AC16FU_06585"
     CDS             <1..331
                     /locus_tag="AC16FU_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994073.1"
                     /translation="SDSTVTDYRCVVCAAKAVARLPKAEIRTRSAVSGKPVTITKRKS
                     EWQVSPKSATFLLLTERDGECLEVHKAFVNEKEFKDYLAGRRELASEKPKPQRIDDLK
                     KKVAPNK"
     gene            328..1149
                     /locus_tag="AC16FU_06590"
     CDS             328..1149
                     /locus_tag="AC16FU_06590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR2994074.1"
                     /translation="MTGSGDRGRSSIFTEKFLATARRAAVAAALEAGNYLVRQFGKRQ
                     AVGYKGAINLVTTADRQAERRIVRRIKKVFPDHSISAEEAEKRDTGSEFRWLIDPLDG
                     TTNYAHGLPIYCVAIGLERSGEIILGVVYNPNLAELFLAVRGRGATLNGQRLRISTTR
                     RLDRSLLATGFPYDIRTSKKNNLEYFRRFALSARAVRRCGAAALDMCYVACGRFDGFW
                     ELKLAPWDVAAASLVVAEAGGTLTDFSGGPFAITKSEVVASNGKIHRQMLAVLGR"
     gene            1245..1700
                     /locus_tag="AC16FU_06595"
     CDS             1245..1700
                     /locus_tag="AC16FU_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR2994075.1"
                     /translation="MRFICDEMLGKLARWLRVLGYDTRYFKKIDDARLVRLALEEGRS
                     ILTRDRRLSQLHLAAGALLLKSDNPLEQLQQVISHFALAREAEARFTRCLACNTLLQE
                     INREAIQDRLWPYIYQTHKEFRFCPTCNKVFWPGTHVRRMEEELELMKK"
     gene            complement(1730..2482)
                     /locus_tag="AC16FU_06600"
     CDS             complement(1730..2482)
                     /locus_tag="AC16FU_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2994076.1"
                     /translation="MGQTKKDKARVLVVDDDARIAALLSDTLAAIGYRTVSATSGSEA
                     LGCIAQTRPDIVITDLKMPEMDGFSLLREIKDKYPDLVVVAITGEILNEVEKQKAERG
                     ADGFLTKPFRIGKIEELIENLLSGAPPKRTGPSCILIVDDDPEVRQSLAKLLVEAGYE
                     TLTAPDADQGLALLATRKINFVITDVVMPGLSGPQLVRIIKSRYPDLPVALITGKTDN
                     IIPPEKDLKAADAFLHKPIRLSDLTKIIGSNT"
     gene            complement(2578..3927)
                     /locus_tag="AC16FU_06605"
     CDS             complement(2578..3927)
                     /locus_tag="AC16FU_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR2994077.1"
                     /translation="MASVPFNPRIFKAYDIRGIYPAEINRESAEWIGRALVGYLRPRQ
                     VVVGRDMRLSSDELFGGLVAGLRAAGTDVVDIGLCSTDALYFAVGKYGYDAGVMITAS
                     HNPKEYNGFKICRREAVPLSAGEGLDELRQRIESGDLSPAKKSGELTQRNITPAFVDH
                     LLSFIAPQKIKPFKIAIDAGNGMAGMLVPEIFSRLSCQLIPLYFELDGSFPNHPASPI
                     EPENLHDLQRKIRETGADLGAAFDGDADRMFLVDEKAKPLGGDVVTALIAKNLLRKNP
                     GATVLYNLICSRLVPEVITRAGGRAVRTRVGHAFIKPLMKEHSAIFGGEHSGHFYFRD
                     NWYADSGMIALLVCLELLSLENRPLSALVREIDPYHRSGELNSRVADQQVVIEKVVGN
                     FSDGLIDQEDGLTLQYPDWWFNLRPSNTEPLLRLNVEANSPELLGEKTALLLSLIRK"
     gene            complement(4052..4819)
                     /locus_tag="AC16FU_06610"
     CDS             complement(4052..4819)
                     /locus_tag="AC16FU_06610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019599421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2994078.1"
                     /translation="MGRVAGKVAIVTGGALGIGQATCLLLAQEGAKVAVTDLLDREGQ
                     TVVDEITRSAGTARFWHMDVSQEAEVRQAVAAIHKTFGKIDILVNNAGIAGVSKPTHE
                     ITEEEWDQVMAVNVKGVFFCTKHVIPYMLAAGGGSIINLSSIYGIISAPDVPPYHASK
                     SAVRSMTKTDALIYGKDKIRVNSVHPGFVWTPMVENYLKSQGDVAAGRKFLDGLHPLG
                     HVGKPDDVAYGILYLASDESKFVTGSELVIDGGYTAR"
     gene            complement(4872..5321)
                     /locus_tag="AC16FU_06615"
     CDS             complement(4872..5321)
                     /locus_tag="AC16FU_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR2994079.1"
                     /translation="MEFEIPLPLRSEHDELHVELARATKVKGKIGTAAQAVATILHSH
                     FVKEEKYALPPLGLLPLLAKGKVTAEMAEVLTLTDRLKAELPQMLQEHRAIVAALDNL
                     LAAAKKEKKSAYARFAKNLMLHAQTEEEVFYPAALLVGEYLKLKLNR"
     gene            complement(5357..5980)
                     /locus_tag="AC16FU_06620"
     CDS             complement(5357..5980)
                     /locus_tag="AC16FU_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR2994080.1"
                     /translation="MKKNLVQRLLALIIVATGVFLGTNQVLAHCDGMDGPVVKAAQKA
                     LETGNVNLVLIWVQKDDEGALKEAFQKTLTVRKLSPDARELADMYFFETLVRTHRAGE
                     GAPYTGLKPAGRDLGPAIPAADKALESGGVEPLMKLLTDTLQAGIRKNFQEALAKKNF
                     TRDDVAAGREYVRAYVEFIHYVERLYAAAHGPAHGHFPEAGMHAEEH"
     gene            complement(6033..7478)
                     /locus_tag="AC16FU_06625"
     CDS             complement(6033..7478)
                     /locus_tag="AC16FU_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR2994081.1"
                     /translation="MNVRLSTGREIPIEMHKVRIVQKTSLAPVSERLKAIAEGGYNTF
                     SLRTRDIFIDMLTDSGTNAMSDNQLAAMMVADDAYAGCESFYKLAAAIKDVLRFDYVL
                     PVHQGRAAEHLLAKVFIKPGDVIPMNYHFTTTKAHFELAGGTVLEIYTNEALNTQSTN
                     PFKGNLDLQKFKDTIKKYGAAKIPFIRMECTTNLLGGQPFSLENLKAVKEIAAEHKIP
                     LVVDGSLLSENAYFIKQREKGYADKSIQEIIWEIMSLADLFYLSGRKSTCVRGGFIAT
                     NNKKFYEDLKPWLPVYEGFLTYGGMSTKEIEAMAVGLREMTEINVAGSAAEFVKYFVE
                     RLVESKIPVLTPPGGLACHLDAKLFLPHLPQSQYPAGALSAAVYLASGVRTMERGTIS
                     MDRDKEGKEVYSDLELVRLAVPRRVYTMSHIEYVVDRVNWLYKHRDLIKGLKFVEEPP
                     VLRFFFGRLQPLDNWGAKLAEVFQKEFGLYY"
     gene            complement(7846..>8155)
                     /locus_tag="AC16FU_06630"
     CDS             complement(7846..>8155)
                     /locus_tag="AC16FU_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR2994082.1"
                     /translation="ADSLLAEAIRTVAALYQDGILKKPKSYAYPFPDLLTFHDAPTVI
                     EQKLFVMHLEHRMRAFQGAFHANPDYALWYGWSEMQRDLTEIKTLAADLRWNHTKTQR
                     "
BASE COUNT         1661 a         2292 c         2330 g         1872 t
ORIGIN      
        1 ttccgattcc acggtgaccg actaccgctg tgtcgtctgc gccgccaagg cagtggccag
       61 gctgccaaaa gcggagatca ggacgcgttc ggcggtctct ggcaaaccgg tgacgattac
      121 taaaagaaaa agcgagtggc aggtgtcgcc gaaatctgcc accttccttt tgctcacaga
      181 gcgggatggc gaatgcctgg aggtgcataa ggcgtttgtc aacgaaaaag aattcaaaga
      241 ttatctggcg ggacggcggg aattggcctc agaaaaaccg aaaccccaac gcatcgacga
      301 cctgaagaaa aaggttgctc cgaataaatg actgggtccg gcgatcgggg tcgttcttcc
      361 atttttacgg agaaattcct tgccacagcc cggcgtgcgg cggtggcggc ggcgctggaa
      421 gcgggaaatt atttagtccg gcagttcgga aagcgccagg cggtcgggta taaaggggcg
      481 atcaatctgg ttacgaccgc cgacaggcag gcggaacgga gaatcgtccg ccggattaaa
      541 aaggtcttcc ccgaccactc gatttccgcg gaagaggccg aaaagcggga taccggctcc
      601 gaattccgct ggctcatcga tcctctggac ggcaccacca actacgccca tggcctgccg
      661 atttactgcg tggcgatcgg gctggagcgc tccggtgaaa ttatcttagg ggtagtctac
      721 aatccgaatc tggcggagct cttcctggca gtccggggtc ggggggccac gttgaatggc
      781 caacgcctgc ggatctccac cacccggcgt ttagatcgga gccttttagc caccggcttc
      841 ccctatgata ttcgcacctc gaagaaaaac aatctggaat acttccgccg ctttgcgctc
      901 tcagcgcggg cagtccggcg ctgcggtgcg gcggctttag atatgtgcta cgttgcctgc
      961 ggccggttcg acggcttttg ggaattgaaa ctggcgccct gggacgtagc ggcggcgagt
     1021 ctcgtggtcg ccgaagcggg cggaaccctc actgattttt ccggcggccc gttcgccatc
     1081 accaaatccg aagtggtggc ctccaacggc aaaattcatc gacagatgct ggcggttttg
     1141 ggccgttaaa gaaccactca cagcagatga agcagattta gcagatagaa aagccggaga
     1201 agtgcggtaa gacttgcgtc tttctatctt cttcccggtg tgccatgcgc ttcatctgcg
     1261 acgaaatgct ggggaagcta gcccgctggc tacgggtttt ggggtacgac acccgctatt
     1321 tcaaaaaaat agacgacgcg cggctggtgc gactcgccct ggaagagggg cgttcgattt
     1381 tgacccgcga ccggcgcttg agtcaactgc acctggccgc cggagcgttg ctgcttaaaa
     1441 gtgacaatcc cctggaacag ctgcagcagg tgatttctca ttttgctctc gcgcgggagg
     1501 ccgaagcccg attcacccgc tgtctggcct gcaataccct gctccaggaa ataaaccggg
     1561 aagccatcca ggaccgcctc tggccgtaca tctaccagac ccacaaagaa tttcgctttt
     1621 gtccgacctg caacaaggtt ttctggccgg ggacacacgt ccggaggatg gaggaagaat
     1681 tagaactaat gaagaaataa acggacagcg aagtgggtct agctcagcgc tacgtgttgg
     1741 agccgataat tttggtcagg tcgctcaagc gtatcggctt gtgcaggaaa gcatcggctg
     1801 ctttcaaatc cttctcgggg gggatgatgt tatcggtctt gcccgttatg agcgccaccg
     1861 gcaggtcagg gtagcggctc ttaatgatcc gcaccagttg cgggccggag agcccgggca
     1921 tcaccacgtc ggtgatgacg aagttaattt tccgggtggc taaaagcgcc agcccctggt
     1981 cggcgtcggg ggcggtgagg gtttcgtagc cggcctcgac caagagtttg gccaaactct
     2041 ggcgcacttc ggggtcgtcg tccacgatca ggatgcagga aggcccggtc cttttgggcg
     2101 gggcacccga gagaagattt tcaatcagtt cctcgatttt gccgatacgg aacggcttgg
     2161 tcaaaaatcc gtcggccccc cgctcggctt tttgtttttc gacttcgttt aagatttcgc
     2221 cggttatggc gaccaccacg aggtccgggt atttatcttt aatttcgcgc aaaagggaga
     2281 aaccgtccat ctccggcatc ttcaggtcgg tgatgacgat gtcgggacgg gtttgcgcga
     2341 tgcagcctaa cgcctccgaa ccactggtgg cgctcacagt ccgatagcca atcgccgcca
     2401 gcgtgtcgga gaggagcgcg gctatgcggg catcatcgtc aacgaccaaa accctggcct
     2461 tgtctttttt ggtctgcccc atcttgagaa ccttctgtct gcattatcgg cagaagtgca
     2521 aaatcgttca ctcccaccag cccctcggtc atgggtaact gcttgcgcga ccggagatta
     2581 ttttctgata agtgataaga ggagggcggt tttttcccct aacaattcgg gtgaattggc
     2641 ctcgacattc aaccgcaaga gcggttcggt gttggaaggg cgcaggttga accaccagtc
     2701 ggggtattgc agggttaagc cgtcttcttg atcgatgagg ccatcggaaa aattcccgac
     2761 caccttttcg ataacgactt gctggtcagc cacccgcgaa ttgagctctc cggagcggtg
     2821 gtagggatcg atttcccgga ctaaagccga aaggggacgg ttctcaaggg acaggagttc
     2881 caggcagacc aaaagggcaa tcatcccgct gtcggcgtac cagttatccc ggaagtaaaa
     2941 atggccggag tgttcccccc cgaagatggc gctgtgttct ttcatcaaag gtttgatgaa
     3001 ggcgtgcccc acccgggtgc ggacggcccg gccgcctgcg cgggtaatta cctccggcac
     3061 cagccgggag cagatgaggt tgtagagcac ggtggctccg gggtttttcc tgagcagatt
     3121 tttggcgatg agtgcggtga ccacgtcgcc gccgaggggt tttgcctttt cgtccaccaa
     3181 gaacatccgg tcggcatcgc cgtcaaaagc agccccaaga tcagcaccgg tttctcgaat
     3241 ttttctctgg aggtcatgca gattttccgg ttcaatgggg ctggctgggt gattggggaa
     3301 cgagccgtcc agttcaaagt acagggggat caactgacag gacagtcggg agaagatttc
     3361 cggtacgagc atccccgcca tgccgttgcc ggcatcaatg gcaattttga acggtttgat
     3421 tttctgcggg gcgatgaatg acaaaagatg atcgacgaaa gccggagtga tgttcctttg
     3481 ggtcagttcg cctgattttt tagccggcga caggtcaccg ctttcaatcc gctggcgcag
     3541 ctcgtctaac ccttcccctg ccgaaagggg gacggcttcc cggcggcaga ttttgaagcc
     3601 attgtactcc ttggggttgt gggaggccgt aatcattaca ccggcgtcat aaccgtattt
     3661 tcccactgcg aagtagaggg cgtcggtgga gcagaggccg atgtctacca cgtcggtgcc
     3721 ggcggctcgc aagcccgcaa ccaatccgcc gaaaagctcg tcggacgaca aacgcatatc
     3781 ccgccccacc accacctgcc gcggtcggag gtagcccacc agcgctcgac cgatccactc
     3841 tgccgactcc cgatttatct ctgcgggata aatgccccgg atatcgtagg ctttgaaaat
     3901 gcggggattg aacggaacag acgccatgac tgctgaatat actgaatgaa aaagactctg
     3961 ctgtggtcaa gaaatatctc ttctagtacc gtttaggaaa aggaggtgaa acgggaatca
     4021 aattttgcga agcaggttga tccagtagaa ttcagcgcgc cgtatagccg ccgtcgatta
     4081 ccagttcgct tcccgtgacg aacttagatt cgtcggaggc gagatagaga atgccgtagg
     4141 ccacgtcgtc cggcttaccc acatgaccca gggggtgcag gccatccagg aatttccgcc
     4201 cggccgcaac gtcaccttga gatttcaggt agttttccac catcggcgtc cagacgaagc
     4261 cggggtggac ggagttgacc cggatcttgt ccttgccgta aatgagggca tccgttttag
     4321 tcatggatcg cacggcgctc ttggaggcgt ggtacggcgg aacgtccggc gcactgatga
     4381 tgccataaat ggaggagagg ttgatgatgc tcccgccccc ggccgccagc atgtaaggga
     4441 tcacgtgctt ggtgcagaag aagactccct tcacgttgac ggccatcacc tgatcccatt
     4501 cttcctcggt tatctcgtgg gtcggcttgc ttaccccggc gatcccggcg ttgttgacca
     4561 ggatgtctat tttcccgaaa gtcttatgaa tagcagcgac tgcctgccga acttccgctt
     4621 cctgggaaac atccatatgc caaaaccggg ccgtgccggc ggagcgagtg atttcatcca
     4681 caaccgtttg tccttctcgg tcgaggaggt ctgtcaccgc aactttagct ccttcctggg
     4741 ccaagagcag acaggttgcc tgcccgatgc ccagtgcgcc gccagtgacg atggctactt
     4801 tgcctgctac tctgcccatg ttcgcctccc tgggttagcc gccgcaggtt ttcaactcct
     4861 gcccgaagcg cctaccggtt gagtttcaac ttcagatact ctccgaccaa aagtgccgct
     4921 gggtagaaga cctcctcctc ggtctgggcg tgcagcatca gatttttggc aaagcgggca
     4981 taggctgact tcttttcttt tttggcggca gcgagaaggt tgtccagcgc cgcgacgatg
     5041 gctctatgct cctggagcat ctgcggcagc tccgctttca ggcggtcggt cagggtcagg
     5101 acctccgcca tctccgctgt gacttttcct ttcgccagga gaggcaagag ccccaggggg
     5161 ggtaacgcat atttttcctc tttcacaaaa tgggaatgca ggatcgtggc tacggcctgg
     5221 gcagcggttc cgattttgcc cttcactttg gtggcccggg ccaactccac atgcaactca
     5281 tcatgctccg atctcaaggg gaggggaatt tcgaattcca ttgtgtgacc tcctttaggg
     5341 agtcaacgac gtcgtcttaa tgctcttccg cgtgcatccc ggcttccgga aaatgcccat
     5401 gcgcggggcc gtgggccgct gcgtagagcc gctcgacgta atgaatgaat tccacgtacg
     5461 cgcgcacgta ctcccgacct gccgccacgt catcccgggt gaaatttttc ttagccaatg
     5521 cctcctggaa atttttgcgg atcccggctt gaagagtatc cgtcagaagt ttcataagtg
     5581 gctccacacc gccactctcc agggccttgt cggcggccgg aatggcgggg ccgaggtcgc
     5641 ggccagctgg cttgaggccg gtatacggcg ccccttcacc ggcccggtgg gtgcgcacca
     5701 aggtctcgaa gaagtacatg tcggccagtt ctctggcatc cgggctcaat ttccttacgg
     5761 tcagggtttt ctggaaggct tccttaaggg cgccttcgtc gtctttctgc acccagatga
     5821 ggacgaggtt cacgttcccc gtttcgagcg ccttctgggc ggctttgacc acgggaccat
     5881 ccatgccgtc acagtgggcc aggacctggt tcgttccaag gaacacgcct gtggcgacga
     5941 ttatcaacgc cagcaatctc tgcactagat tctttttcat cccattatcc tttccccgtc
     6001 gcaatgacgg tcgggtttgg ttcaaaactg ccttagtagt acaatccgaa ttccttttga
     6061 aaaacctcgg ctaattttgc accccagttg tccaacggct gtaagcggcc gaagaaaaac
     6121 ctgagaaccg gcggctcttc cacaaatttc aatcccttga tcaggtcacg gtgcttgtag
     6181 agccagttca cccggtcaac cacgtattcg atatgcgaca tggtgtagac ccggcggggc
     6241 acggccagcc ggacaagttc taaatctgaa taaacttctt taccctcttt gtcccgatcc
     6301 atcgagatgg tgccccgttc catcgtccgc accccggagg caaggtaaac cgccgccgac
     6361 aacgccccgg ccgggtactg ggattgcggc aggtggggta aaaagagctt tgcatcgagg
     6421 tggcaggcca ggccacccgg cggggtgagc accggaatct tgctctcaac caagcgctcg
     6481 acaaagtatt tcacgaattc ggccgcactg ccggccacgt tgatctcggt catctcccgc
     6541 agtcccaccg ccatcgcctc gatctctttg gtggacattc cgccgtaggt taagaaccct
     6601 tcgtagaccg gcagccaggg cttgaggtcc tcgtaaaatt tcttgttgtt ggtggcgatg
     6661 aagccgcccc gcacgcaggt gcttttccga ccggagaggt agaagagatc cgccagactc
     6721 atgatctccc agatgatctc ctggattgac ttgtccgcat accccttttc gcgctgctta
     6781 atgaagtagg cattctccga gagcagactg ccatcgacca ccagaggaat cttgtgctcg
     6841 gcggcaatct ctttcaccgc tttcaaattt tccagagaaa agggctgtcc acccaggaga
     6901 ttggtggtgc actccatgcg gatgaagggg atttttgcag ccccgtattt tttaatcgta
     6961 tccttgaatt tctgtaagtc caggttgcct ttaaacgggt tggtgctctg ggtgttcaag
     7021 gcttcattgg tgtaaatttc cagtaccgtg cccccggcca gctcaaagtg agctttggtg
     7081 gtggtgaagt ggtagttcat cgggatgacg tctcccggct tgatgaagac cttggctaag
     7141 agatgctcgg cggcccgccc ctgatgcacc ggcaggacgt agtcgaaccg taaaacgtct
     7201 tttatcgccg cagccagttt gtaaaaactt tcgcagccgg cgtaggcgtc gtcggcaacc
     7261 atcatggcgg ccagctggtt gtcgctcatc gcattcgtcc cgctatcggt gagcatatcg
     7321 atgaagatgt ctctcgtccg gagggagaag gtattgtacc cgccttctgc tatcgccttg
     7381 agtctttcgc tgaccggggc cagactggtt ttctgcacga tccgcacctt gtgcatctcg
     7441 ataggaatct ctctgccggt ggaaagtcgg acattcatct ggggctcctt tgctgattag
     7501 attctcattt cacgaaaata ttcggaagtt tctgtgctct attcttcgtc actgcctcgc
     7561 gattcttccg ttgccgatgg ttcaattcaa gaccgcgctc ctggctgccg gcctctcgcc
     7621 ggcgacggct ctcctctgcc atacggagga atttaagagt tttagacaaa aaaagccact
     7681 tttcccctta ggtcgctgtg gcttttcctg tactcctccg ttcggtgaaa tggatggaaa
     7741 gaagcccccc taaccgtcta ctatggcgcg cagacggcta tcggagcacc ggcacacaga
     7801 ccggccggtt cactgtcagc ccctctcccg gagtgagact gcttctcacc tttgagtctt
     7861 tgtgtgattc cacctgaggt cagccgccag cgttttgatt tcagtgaggt cgcgctgcat
     7921 ttcgctccag ccgtaccaga gcgcgtagtc cgggttggcg tgaaaggccc cttggaaggc
     7981 gcgcatccgg tgctccaggt gcatcacgaa gagtttctgc tcaatcaccg ttggcgcgtc
     8041 gtggaaggtc aggagatcgg ggaacggata ggcgtagctt ttcggttttt tcagaatccc
     8101 atcttggtag agtgcggcga cggtccgaat cgcctcagcc aacagactgt ccgcc
//
LOCUS       DBNGZA010000303         4400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2815078, whole genome shotgun sequence.
ACCESSION   DBNGZA010000303 DBNGZA010000000
VERSION     DBNGZA010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2815078"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..749)
                     /locus_tag="AC16FU_06635"
     CDS             complement(36..749)
                     /locus_tag="AC16FU_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994083.1"
                     /translation="MEGFTFGTTLAAILTIGILSFLYKDNPLYKLCESIFIGISAGYW
                     FVSLYFQNMLPKMFDNLGITKALGSKEAGDGALQVLLHQHHWDERWIFLVAGVLGIMM
                     LLRLFPKIGWISRWPLAFVVGYTSGLYFIAYLQSNVFAQVQKSVVPLATLAQSNGAIA
                     WGPTIGTLVVVFGTLAGLVYFFFSKEHKGFFGGTARLGIWFLMVTFGAAFGYTVMSRM
                     SLLLGRLDFLFGTWLGLVP"
     gene            complement(753..1625)
                     /locus_tag="AC16FU_06640"
     CDS             complement(753..1625)
                     /locus_tag="AC16FU_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994084.1"
                     /translation="MAEVPNVPETQRGFFDRLVAIDRRVIFLFVGLALLVPLFMNVSQ
                     KMKISPEVQSLFDAMQALPPGSKVLLACDYDPPSSPELQPMAVTAVKYCFLYRLKMIV
                     IGLWPQGPQQADLAIRAALKDPEVAKVNPQYGIDYCNLGFQAGNEVVIQKMGSDIPGT
                     FPKDWRGTDVKTLPIMTGIKNFSNIDFIFNLSAGYPGTREWVQLAADRFHAKLVSAAT
                     AVQTPEYTPYLGRQLLGILGGMKGGAEFEALTTYRGKATKFMLAQSLSHLIVVAFIII
                     GNAAYFASRKKKGA"
     gene            complement(1625..2278)
                     /locus_tag="AC16FU_06645"
     CDS             complement(1625..2278)
                     /locus_tag="AC16FU_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994085.1"
                     /translation="MRREIPLILTAIVGLTLVIQFFIPHEPFNKMQTWFADWVAIIQA
                     FAIWLGALNLLVVSAEKVSKKQPGWFYSVVIIASFFLIVIPGLFFSGGRHFQEAGTSF
                     DWLYAHMFTPLSATMFALLAFFVASASYRAFRARNKEATLLLLAGIFVMLGRVPIGDV
                     LTNFLPEKIRLSSVADWIMAYPQLAGQRAIMIGIALGIVSTSLRIILGIDRTIVGGK"
     gene            complement(2285..3445)
                     /locus_tag="AC16FU_06650"
     CDS             complement(2285..3445)
                     /locus_tag="AC16FU_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2994086.1"
                     /translation="MRIFFLAVLLSLAWVLPAWAIAPPLDVQAKDTPNDAGKSITVTW
                     KKSPDDRPGVAGVAEYRILRGTSLDGPSTFVGTATGGTELFTDNTAQNNINYYYKIVA
                     VAGDKSEAESLPSAPTHAYQQWFNAKRVNLLIGVLLLSFAIIYFIEQAKKGKPLFIRK
                     IAGLEAVDEAIGRATEMGKKVLYIPGIQDLDNVQTVAGVTILGRVAKVAAEYETHLEV
                     PTSRALVMVACREAVQEGYSLAGRPDAYNQDRIYYLTDEQFGYAAGVDGIIVREKPAA
                     IFYQGCFFAESLILSETGNSIGAIQIAGTAQPTQLPFFFAACDYTLIGEELFAASAYL
                     SREPRQLGSLKGQDIGKALIMLAVLAGAIWQTLAVFFPETFHANAASLFLTE"
     gene            complement(3747..4355)
                     /locus_tag="AC16FU_06655"
     CDS             complement(3747..4355)
                     /locus_tag="AC16FU_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR2994087.1"
                     /translation="MRKILFVRALCLIGITIAAAQQPEKSKSGQPEGQSLGMMMPKPG
                     PELAKLDYFLGTWTGEETLNYPGMPAPAKSKSKYTAKRGVGGMFLMGDYNATMSDGKT
                     EQKMEAHTLWTYDPQQKNYRLWGFDSFGTAMEMRGNWTDDKTFVAETSTEMGGMPVKL
                     RDTYTLVSPTTCTLKSEMDMGQGWQTGMEGTYTKQTETKKPK"
BASE COUNT          962 a         1264 c         1277 g          897 t
ORIGIN      
        1 tcgtctcttg agtgaacgcg ccgcttctcc cgtactcacg gcaccaaccc cagccaggta
       61 ccgaagagga aatcgagccg gcctaaaagc agcgacatcc gcgacatgac cgtgtagccg
      121 aatgcggccc cgaaagtaac catcagaaac cagatcccca gccgcgccgt tccgccgaaa
      181 aaccctttat gctctttgga gaaaaagaag tagaccagac cggccagagt tccgaagacg
      241 accaccagag ttccgatcgt cggcccccag gcaatggcac cgttcgactg ggccagcgtg
      301 gccaaaggta cgaccgactt ctgcacctgg gcgaaaacgt tcgactgcag gtaggcgatg
      361 aaataaagtc cggaggtgta gccgacgaca aacgccagag gccaccggga aatccagccg
      421 attttgggaa ataagcgcag gagcatcatg atgcccaaaa cgccggccac caggaaaatc
      481 cagcgctcat cccagtggtg ttggtgcaaa agaacctgca gtgccccatc gcctgcttct
      541 ttggagccga gcgctttggt tatgcccaaa ttgtcgaaca ttttgggcag catgttctgg
      601 aagtagaggg aaacgaacca gtagccggcc gaaatcccga tgaaaatcga ctcgcagagt
      661 ttgtagagcg gattatcctt atagaggaag gagagaatcc cgatggtgag gatggccgcg
      721 agggtggtac cgaaggtgaa gccttccatg ccctatgcgc ctttcttctt ccggctggcg
      781 aagtaggctg cgttcccgat gatgataaag gccaccacga tcaggtgcga gagcgactgc
      841 gccagcatga acttggttgc cttcccgcgg taagtagtga gcgcctcgaa ctccgctcct
      901 cctttcatcc cgcccaagat gcccaggagt tgccgcccca gatacggggt gtactccggc
      961 gtctgcaccg ccgtggcggc ggaaacgagt ttggcgtgga agcggtcggc ggctaactgc
     1021 acccactccc gcgttccggg gtatcccgcc gagaggttga agatgaaatc gatgttgctg
     1081 aagttcttga tcccggtcat gatagggagc gttttcacgt cggttccccg ccagtctttg
     1141 gggaacgtac ccggaatatc cgaccccatc ttctggatca ccacttcgtt gccggcctga
     1201 aaacccaaat tgcagtagtc gattccgtat tggggattta ccttcgctac ctccggatcc
     1261 ttcagcgctg cccggatggc caaatcggcc tgctgcggcc cctgcggcca gagaccaatg
     1321 acgatcattt taagccggta caggaaacag tacttgacgg cggtgaccgc catcggctgc
     1381 agttccggcg acgagggtgg gtcgtaatca caggcgagca aaactttgga ccccggcggc
     1441 agggcttgca tggcgtcgaa gagcgattgc acttccgggg aaattttcat cttctgggaa
     1501 acgttcataa acaagggtac gaggagggcc agcccgacaa aaagaaaaat gacccgccgg
     1561 tcgattgcca ccagtcggtc gaagaacccg cgctgcgttt cgggaacgtt cggaacttct
     1621 gccatcactt tccgcccacg atggtgcggt caatccctaa aatgatccgc agggaggtgg
     1681 agacaatccc taaggcgatg ccgatcatga tggccctttg cccggctaac tgcgggtagg
     1741 ccatgatcca gtcggccacc gacgagagcc tgattttttc cggcagaaaa ttcgtgagca
     1801 catccccgat gggcacccgg cccagcatga caaaaatccc ggccagaagc aaaagcgtgg
     1861 cctctttatt gcgggcgcga aaggcgcggt aggaggcgga agcaacgaaa aacgccaaaa
     1921 gcgcgaacat cgtggccgaa aggggggtga acatatgggc gtagagccag tcgaaggagg
     1981 tcccggcctc ctggaagtgc cgcccaccgg agaagaagag tccggggatg acgatcaaaa
     2041 agaaactggc gatgatgacg acggagtaga accatcccgg ctgctttttg gagactttct
     2101 ctgccgagac aaccaaaagg ttcaacgctc ccagccagat ggcaaaagcc tggatgatcg
     2161 ccacccagtc ggcgaaccag gtctgcatct tgttgaacgg ctcgtgcgga ataaagaact
     2221 ggatcacgag cgtgagcccg acgatggcag tgaggattaa gggaatttcg cgccgcatag
     2281 agttttattc ggtcaggaaa agactggccg cgttggcgtg aaatgtctca gggaagaaga
     2341 ccgctaacgt ttgccagatc gctccagcca gaacggccag catgattaag gctttgccga
     2401 tatcctgccc cttgaggctg cccaattgcc ggggctctcg cgagaggtaa gcggaggccg
     2461 caaacagttc ctcgccgatg agcgtgtagt cgcaggcggc aaagaaaaag ggcagctgcg
     2521 tcggctgggc cgtgcccgcg atctgaatgg cgccgatgga attgcccgtt tcggaaagaa
     2581 tcaaagactc ggcgaaaaag catccctggt agaagatcgc cgccggtttc tcccgcacga
     2641 tgatcccgtc aacgccagcg gcgtagccga actgctcgtc agtcaagtag taaatccggt
     2701 cctgattgta agcgtcgggc cgcccggcca gggagtaccc ctcctgcacc gcttcccggc
     2761 aggccaccat gacgagcgcg cgtgacgtgg gcacttctaa gtgtgtttcg tactcagccg
     2821 cgactttggc cacgcggcct aaaatcgtca cgccggccac ggtctgtacg ttatccagat
     2881 cttgaatacc gggaatgtag agaactttct tgcccatttc tgtggcccgc ccgatcgctt
     2941 cgtcgacagc ttccagcccg gcgatcttgc ggatgaagag cggcttccct tttttggcct
     3001 gttcgataaa atagatgatg gcgaaagaca gaagcagcac tccgatcagg agattcaccc
     3061 gtttggcgtt gaaccactgt tggtaggcgt gcgttggcgc gctggggagc gattcggcct
     3121 cgcttttgtc gcctgcgacc gcaacgattt tgtagtagta attgatgtta ttctgagccg
     3181 tgttgtcggt gaagagttcc gttccgccgg tggcggtgcc gacaaaggtg gacggcccgt
     3241 ccagactagt gccgcgcagg atacggtact ccgccacccc ggccacgccg ggccggtcgt
     3301 ccggcgattt tttccaggtg acggttatgc ttttgccggc atcgttgggc gtatctttgg
     3361 cttgaacgtc cagaggcggc gcgatagccc acgccggcaa aacccaggcg agggacagga
     3421 gtaccgccag aaagaaaatt ctcaccggag ctgtcaaccg catagggatt gtccgaaaac
     3481 aaaataccac actttgttga agtgtggcga ctccattggc attgcgaccg gcgaacccgt
     3541 ggctgtcagt tttcctccat tcggtacatt gctcccgctg ctggcggaag gcggctcaaa
     3601 atctaaaact ctcgtcgaac gctgtcaagc gaaaaaagag aaacgcagac acccgatctc
     3661 ctgcgtccgg ctgccagcaa aatggctccg caaatgaaaa accgggcatc tctcgccaga
     3721 cgcccggcgg cggacggaaa gaccgcttac ttgggtttct tggtttcggt ctgcttagtg
     3781 taggtcccct ccatgccggt ctgccacccc tggcccatat ccatttcgct tttcagcgtg
     3841 caggtggtcg gggagacaag cgtgtaggtg tcccgcagct tgaccggcat gccgcccatc
     3901 tcggtggagg tttccgccac aaaggttttg tcgtcggtcc agttgccgcg catctccatc
     3961 gcggtcccaa aggagtcgaa tccccacagc cggtaatttt tctgttgggg atcgtaggtc
     4021 cagagcgtgt gggcttccat cttctgttca gttttcccat cgctcatggt ggcgttgtag
     4081 tcgcccataa ggaacatccc gccgactccc cgtttggccg tgtacttcga cttgctcttg
     4141 gccggtgccg gcatgcccgg gtaattgagc gtctcctcgc cagtccaggt gcccaagaag
     4201 tagtcgagct tggccagttc cgggcccggt ttgggcatca tcatccccag cgactgccct
     4261 tccggctgcc ctgacttgct tttctccggc tgctgcgccg ccgcaatggt tatacctatc
     4321 aaacagagtg cgcgcacgaa aaggatttta cgcattcctc ctccttattc gcctgtgtgg
     4381 acgattactt tctgggtttc
//
LOCUS       DBNGZA010000304         1237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_270095, whole genome shotgun sequence.
ACCESSION   DBNGZA010000304 DBNGZA010000000
VERSION     DBNGZA010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_270095"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16FU_06660"
     CDS             complement(<1..545)
                     /locus_tag="AC16FU_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994088.1"
                     /translation="MRTGIALAAVLAVILVVVAYFSIQKSQKDLLEVLRRQGIALIES
                     LTLASQNTVRSNALVEALIDDRLTDLAGLIVQLAHAGKYSPALLKQIADQQGVERIDL
                     FGAKKNLLNSTAPDEPFYLQQTDVSALVDSVIWGYRKSIIVQLQPEPLHPEEHLALVT
                     AAPGLAKAMVIVTTLEFLGDFR"
     gene            complement(782..>1237)
                     /locus_tag="AC16FU_06665"
     CDS             complement(782..>1237)
                     /locus_tag="AC16FU_06665"
                     /inference="COORDINATES: protein motif:HMM:NF018533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR2994089.1"
                     /translation="PRLTSQGLDAIDPALTLDVFKERLRKYHGEIKGILTRGELVAGI
                     GNAYADEILWEAKIYPFRKRKTLSETDVQALYEAIGKVFREAVAILQKRVGSNIHLEL
                     RDFLKVHRRGKQLCPRCGEPISDVYAKQKVTSFCRKCQPGLLLDNLRRR"
BASE COUNT          254 a          334 c          383 g          266 t
ORIGIN      
        1 cggaagtcgc ctaagaattc gagcgtcgtg acaatcacca tggccttggc caatcccgga
       61 gcggcagtga ccaaagccag gtgctcttcc ggatggagcg gttcgggctg caactggacg
      121 atgatcgatt tccggtagcc ccagatgacc gaatcgacca gcgcactgac atcggtctgc
      181 tggaggtaga acggttcgtc gggtgccgtg ctgttgagga gatttttctt ggcgccgaag
      241 aggtctatcc gctctacgcc ctgttggtcg gcgatttgtt tgaggagcgc gggggagtac
      301 ttgccggcgt gcgccagttg cacaatcagg ccggccaggt cggtgaggcg gtcgtcgatt
      361 aacgcttcca ccagcgcgtt cgaccggacg gtgttctggc tggccagagt caggctttcg
      421 attagggcaa ttccctgacg gcgcaggacc tccagcaagt ctttttgact tttctggatt
      481 gaaaagtaag cgacgacgac aaggattacc gccaaaaccg ccgccagcgc aataccggtc
      541 ctcaaacgga ggcgcgccag aaggcgcaga aaaggagatt ctcttacggg cacaccgtga
      601 aactaaccgg cttggcggac gagtgtcaag ataggagaag ttggggttag tcttgctact
      661 atgacctcac ccccaccctc tcctacagga gagggagtgt aaaccttttc aaaagaaagt
      721 tgaatctccc tacccttgag ggtcgaagac cccgcctctg gcgggggagg gcaggggggg
      781 tctatctccg gcgcaggttg tccagcagca accccggctg gcacttccgg cagaaagagg
      841 tcactttctg cttggcgtac acatccgaaa tcggctcgcc gcagcgcgga cagagttgct
      901 tgccccgccg gtggactttg aggaagtcgc gcagttccaa gtggatgttg ctgccgacac
      961 gcttctgcaa gatggcaacg gcttcgcgaa atactttccc gatggcttcg tagagtgctt
     1021 gcacgtcggt ttccgaaagg gtcttccgtt tgcggaaagg gtagattttc gcttcccaga
     1081 ggatttcgtc ggcgtaggcg ttgccgatgc ccgccactag ttcgccgcgg gtgaggatgc
     1141 ccttgatttc accgtgatat ttgcgcaggc gttctttgaa aacgtccaag gtcaatgccg
     1201 ggtcgatagc atccaatccc tgagaagtca agcgggg
//
LOCUS       DBNGZA010000305         1134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_59046, whole genome shotgun sequence.
ACCESSION   DBNGZA010000305 DBNGZA010000000
VERSION     DBNGZA010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_59046"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..400)
                     /locus_tag="AC16FU_06670"
     CDS             complement(20..400)
                     /locus_tag="AC16FU_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2994090.1"
                     /translation="MFKQVDYVMVMVSDMARSVAFYRDKLGLPLKFESKDWTEFQTGA
                     TTLALHGGGVGQPPKPPNPGASFAGTCSIGFTVPDVEKSFQDLKAKGVNFVMPPKRQE
                     DEDIILAVCTDPDGLPVSLAQHIQ"
     gene            complement(424..>1134)
                     /locus_tag="AC16FU_06675"
     CDS             complement(424..>1134)
                     /locus_tag="AC16FU_06675"
                     /inference="COORDINATES: protein motif:HMM:NF039902.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR2994091.1"
                     /translation="ESVTEFRDRGFLPETVLNFLALLGWSPGDEREILSRKELIDLFS
                     LERVTQANPIFDMQKLEWMSGEYLRALPDEQLLDCVLPHLQAAGLVTQEAAQKKREWL
                     VRLIPLLKERARTLVDFASVGRYFFTDQFDYDPQGVAKQFADPQAAERLAELAKRFRD
                     LSLFNKETADEALRSYALALGVKPALLIHPTRLALTGVTMGPGLFDIVATLGQAEVVK
                     RLEKAVVFLRTQKPVSIR"
BASE COUNT          246 a          336 c          322 g          230 t
ORIGIN      
        1 ccggtcaagt cggcgccgct tactgtatat gttgcgccag agaaacgggc agcccgtcgg
       61 gatcggtgca gaccgccaaa atgatgtctt cgtcttcctg ccgcttgggc ggcatcacaa
      121 aattgacgcc cttcgccttc aagtcctgga aacttttctc gacgtcggga accgtgaagc
      181 cgatagagca ggtgccggcg aaggaagctc cggggttggg cggcttcggg ggctgaccga
      241 ccccgccgcc gtgcagagcg agtgtcgttg cgccggtctg gaattcggtc cagtctttgg
      301 attcgaactt caacggcagt cctaatttgt cgcggtagaa cgcgaccgaa cgcgccatat
      361 cggaaaccat caccatgaca taatcaacct gtttgaacat cactacctcc tcatttgaga
      421 ctttcagcgg atagagaccg gtttctgcgt ccgcagaaat acaaccgcct tttctaaccg
      481 cttgaccact tcggcctggc ctaaagtggc gacgatgtca aaaagccccg gccccatggt
      541 gaccccggtc aacgccagcc gggtgggatg gattaaaagt gccggtttga cgccgagtgc
      601 aagagcgtag ctgcgcagtg cctcatccgc agtttcttta ttgaataaag agaggtcgcg
      661 aaaccttttg gcgagttctg ccagccgttc cgctgcctgg gggtcggcaa actgcttggc
      721 cacgccctgc gggtcgtagt caaactggtc ggtaaagaag tagcgcccga cggaagcgaa
      781 atcaaccagc gtccgggcgc gctctttgag caacggaata aggcgcacga gccactcacg
      841 cttcttctgc gcggcttcct gagtcacgag acccgcggcc tgcaggtgag ggaggacgca
      901 atcgagcagc tgctcatccg gcagggcgcg caggtactcc ccgctcatcc actccaattt
      961 ttgcatgtcg aagatcggat tggcctgtgt cacccgctct aaggagaaaa gatcgattaa
     1021 ttctttccgg gaaagaatct ccctctcgtc cccgggcgac cagcctaaca gcgccaagaa
     1081 attcaaaacc gtttcgggca aaaagccccg gtcgcggaac tcggtgaccg actc
//
LOCUS       DBNGZA010000306         4254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1330833, whole genome shotgun sequence.
ACCESSION   DBNGZA010000306 DBNGZA010000000
VERSION     DBNGZA010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1330833"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="AC16FU_06680"
     CDS             complement(<1..716)
                     /locus_tag="AC16FU_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994092.1"
                     /translation="MKIPFFYSYRNLFARRLTTGFTVLGLALVVFVFCAVLMLAYGLK
                     KTLVATGSDDNAIIIRKASQTEMQSYITRDQVAVIESQPEITRGADGKPLVAGELYVL
                     ISLNKRKGGDAANVVVRGVGPKSKLIHANVKLIAGRMFTEGLPEVMVGKAAAERFRGA
                     ELGGTVRFGKEDWRVVGVFAANRSGFESEVWGDVNQMLAAFNRPVVSSMTLKLADPHL
                     FPELKKRLEGDPRLSVDVKQ"
     gene            complement(713..1870)
                     /locus_tag="AC16FU_06685"
     CDS             complement(713..1870)
                     /locus_tag="AC16FU_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994093.1"
                     /translation="MKIVKLAVKNAGRHKLRTALTILGLAVAVMAFGLLRTVISAWYA
                     GAEASAPDRLVTRNAVNIIFPLPLAYREQIQKVPGVEKVSYAVWFGGYYVDPKNFIAN
                     FAIDHPINFDLFPEIIIDSASLRAFQSERSAAICGQILADRFRWKVGDKIRLVGTIYP
                     GNWDLTLRGIYHGRDKSTDESALFLRWDYLDENLRATAPVRAGNVEWYVMKIANPNDA
                     ARVSAAVDALFQNSLAETLTETEKEFNLSFIAMSGTIITALQFISYLIIGIILLVLTN
                     TMAMTARERISEYAVMKSLGFRPYHLAGVIAGESFFIAAAGGILGVVLIFPMAAGFGA
                     AMKAFFPVFQVKPQIAVLAGSFALGVGFLAAVFPILKATRLSIVKGLRTIG"
     gene            complement(1867..2541)
                     /locus_tag="AC16FU_06690"
     CDS             complement(1867..2541)
                     /locus_tag="AC16FU_06690"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994094.1"
                     /translation="MAESIVEIEALSKSYYRDSLEIPVLHNLNFSIPEGEFVALMGPS
                     GSGKTTLLNMIAGIDRPTKGNVKVAGTDLGRLGEGELARWRARHIGFVFQFYNLMPVL
                     TAYENVELPLLLTNLSKKERRKHVETTLGIVGLADRMKHYPRQLSGGQEQRVAIARAI
                     VTDPTLLVADEPTGDLDKQSAEEIMTLLARLNAEYKKTVVIVTHDPRAADKAHVQRHL
                     DKGELS"
     gene            complement(2561..3742)
                     /locus_tag="AC16FU_06695"
     CDS             complement(2561..3742)
                     /locus_tag="AC16FU_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994095.1"
                     /translation="MVKEQADLSSLRIERSSDYRPPRKWWRWVLGLSIVPLLVIGYLF
                     VMHQVAPGLAVQVGTATLITGSQAQALLTASGYVVAQRKAAVASKATGRLEYLGVVEG
                     DRIKAGMVIARLEKADITAELDRAKANLAQAVAESTEATLFYERQKKMVADSLISNLE
                     FEGAEARYQRALAGVASGRAAVKAAEVALENTVIRAPFDGTVLTKSAEVGEMVAPFAS
                     TASSRGAVVTLADMNSLEVEADVAEANIERVQVGLPCEITLDAYPDTRYPGKVSKIVP
                     TADRTRATVLVKVAFLSRDRRVLPEMSAKVNFLPENTQLSAQNTEPVVAVPNSAVTMR
                     NGVKMVFLVEGNLVKSVPVTTWRVLGDMTEIKAGLQAADRVVISPPGSLATGMKVKTS
                     E"
     gene            complement(3815..>4254)
                     /locus_tag="AC16FU_06700"
     CDS             complement(3815..>4254)
                     /locus_tag="AC16FU_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994096.1"
                     /translation="GQVQIGSIAFDHQFQKIAQRSHPGSFRLRSKREQSCFWYYRQVA
                     GILKEMAPTGGFPGPGLGRRKKGPLGEGRRAGRFLFTGPKSAWTCTNSRIAGGVNALS
                     RAVRRTGWLLEFFSPQFGHRRPGSICIAGEDALLFIYPGDRIS"
BASE COUNT          859 a         1320 c         1219 g          856 t
ORIGIN      
        1 tcctgtttga cgtcgaccga cagacggggg tctccttcca gccgtttctt cagttccgga
       61 aacaggtgcg ggtcggccag tttcaaggtc atggaagaaa ccaccggccg gttgaaggcg
      121 gcgagcatct ggttgacgtc gccccagact tccgattcaa agccggagcg gttggccgcg
      181 aaaacaccga ccacccgcca atcctctttg ccgaaccgga ccgtgcctcc cagttcggcg
      241 ccccggaacc tttcggcggc ggccttcccg accatcactt ccggcagtcc ttcagtaaac
      301 atccttccgg cgatgagttt gacgtttgcg tggatcagtt tcgattttgg acccacaccc
      361 cgcacaacga cattggccgc gtcgccgccc ttccgcttgt tcaaactgat cagcacgtac
      421 agctctccgg ccaccagggg tttgccatcc gctccccggg tgatttccgg ttgggattca
      481 atgaccgcca cctgatcgcg ggtgatgtag ctctgcattt cggtctggga ggccttccgg
      541 atgatgatgg cgttgtcgtc ggagccggtg gccaccagag tctttttcag tccgtaggcc
      601 agcatcaaga ccgcgcagaa gacgaagaca accagagcga gacccagaac ggtgaaaccg
      661 gtggtgagcc gccgggcgaa gagattccgg tagctgtaga aaaagggtat tttcatccga
      721 tggtcctcaa gcccttgacg atggagagcc gcgtcgcctt caaaatcgga aagaccgccg
      781 ccaggaaacc gacgcccagg gcgaaagacc cggccaggac ggcgatttgg ggtttgacct
      841 gaaaaaccgg gaagaaagct ttcatcgccg cgccaaagcc ggccgccatc gggaaaatca
      901 agacgacgcc cagaattcct cccgcggcgg cgatgaaaaa ggattccccg gcaatcaccc
      961 cggccaggtg gtacggccgg aaccccaggg atttcatgac cgcgtactct gagatccttt
     1021 cgcgcgccgt catcgccatg gtgttcgtca aaaccaagag aatgatacca atgatgaggt
     1081 aggagatgaa ctggagagcc gtgatgatgg tgccggacat ggcgataaaa gaaaggttga
     1141 actccttctc cgtctcggtc aaggtctccg ccagggagtt ctggaagagc gcatccaccg
     1201 ccgccgagac gcgggcggcg tcgttcggat tggcaatctt catgacgtac cattcgacat
     1261 tccccgcccg tacgggcgcc gtggcgcgca ggttttcgtc caaataatcc cagcgcaaaa
     1321 agagagccga ctcgtcggtc gacttgtccc gaccgtggta aatcccgcgc agggtcaggt
     1381 cccagtttcc cggatagatc gtccccacca gccggatttt gtcccccacc ttccagcgaa
     1441 agcggtcggc cagaatctgg ccgcagatgg cggcactccg ttcggattgg aaggcccgca
     1501 gggaggccga atcgatgata atctcaggga aaaggtcgaa gttaattgga tggtctatgg
     1561 cgaaattggc gatgaaattc ttggggtcaa cgtaatagcc gccgaaccaa acggcgtagg
     1621 aaactttttc cacccccggc accttctgga tttgctcccg gtaggcgagg ggtaagggga
     1681 agatgatgtt gacggcgttg cgggtgacca gccgatccgg agcggaggcc tccgctccgg
     1741 cgtaccaggc cgagatgacg gtgcgcaaga gcccgaaggc catgaccgcc acggccaggc
     1801 cgaggatcgt caaagccgtg cgcagtttgt gccggccggc atttttgacg gccagtttga
     1861 cgattttcaa gacagttccc ctttatcgag gtgcctttgg acgtgggcct tgtccgccgc
     1921 gcgcgggtcg tgagtgacga tcacgacggt tttcttatac tcggcattca ggcgggcgag
     1981 cagggtcata atttcttccg ccgactgctt atccagatca ccggtcggct cgtccgccac
     2041 gagcaaggtc gggtcggtga caatggcccg ggcgatggcc acccgttgct cctgtccgcc
     2101 ggagagctgc cgggggtagt gcttcatccg gtccgccagc cccacaatgc caagcgtcgt
     2161 ttcgacgtgc ttccgacgtt cttttttcga cagattggtc agcaggaggg gcaactcgac
     2221 gttttcatac gcggtcagga ccggcatcag gttataaaac tgaaagacga agccgatgtg
     2281 ccgcgcccgc cagcgggcca attccccttc gcccagccgg cctaagtcgg tgccggcgac
     2341 tttgacgttg cccttcgtgg ggcggtcaat cccggcaatc atattcaaaa gggtggtctt
     2401 gccggagccg gacggcccca ttaaagccac aaactcccct tcgggaatgg aaaaattcaa
     2461 attgtgcagc accggaattt ccagactgtc ccggtagtac gatttggaaa gcgcttcgat
     2521 ttcgacgatt gattcggcca taggttcctc tacggttgaa ctactccgac gttttcacct
     2581 tcatgccggt ggctaagctg cccggggggg agatcaccac ccggtcggcg gcctgcagac
     2641 cggctttaat ctcggtcata tctcccagaa cccgccaggt ggtcaccggc acgcttttga
     2701 ccaaatttcc ctctaccaga aaaaccatct tcactccgtt gcgcatggtc accgccgagt
     2761 ttggcacggc cactaccggc tcggtatttt gagccgaaag ttgggtattc tccggcaaaa
     2821 aattaacttt ggcgctcatt tccggcaaca cccgccggtc acgggagagg aaagcgactt
     2881 tgaccagcac ggtagcccgc gtgcggtcag ccgtcgggac aattttcgac accttccccg
     2941 ggtagcgggt atccgggtag gcgtccaggg taatttcgca gggcagtccc acctgcaccc
     3001 gctcgatatt cgcctcggcc acgtcggctt cgacttccag cgaattcata tcggccagtg
     3061 tcaccaccgc cccgcgcgaa gaagcggtcg aagcaaaggg cgccaccatt tcgcccactt
     3121 ccgccgactt ggtcagaacc gttccgtcaa agggcgcccg gatgacggtg ttctccaggg
     3181 ccacttccgc cgccttcacc gccgcccggc cggaggcgac tccggccagc gcccgctggt
     3241 agcgcgcctc ggcaccctca aattccaggt tgctgatcaa agaatcggcc accatttttt
     3301 tctgcctttc ataaaacaaa gtcgcctcgg tcgattccgc cacggcttgg gccagattgg
     3361 ctttggcgcg gtcgagttcg gcggtgatgt cggccttttc cagccgggcg atgaccattc
     3421 ccgctttgat ccggtcgcct tccaccacgc ccaagtactc cagccgcccg gtggccttgg
     3481 aggccaccgc cgctttgcgc tgggccacca cgtagcctga ggcggtcaaa agcgcttgcg
     3541 cctgactgcc ggtgatcaag gtcgcggttc ccacctggac ggccagcccc ggcgccacct
     3601 ggtgcataac gaagaggtag ccgatcacca gaagcggaac gatcgaaagt cccagaaccc
     3661 agcgccacca cttccgcggc ggccggtagt cggagctccg ctcaattcgc agagaggata
     3721 aatcggcctg ttctttgacc attttcttcc cggttgcacc gccaaattcg ggatgaacgg
     3781 tgctgtcccg ccggcgtttt tttgaccgtt cgcattaaga aatccggtcg cccggataga
     3841 taaaaagtaa tgcatcctcc ccggctatgc aaatactgcc gggtcgccgg tgtccgaatt
     3901 gcggggagaa gaactccaac agccaaccag tccgacgaac cgctcgactt aatgcattta
     3961 ctccgcccgc gatgcgggaa ttcgtgcagg tccacgcact ttttgggccg gtaaagagaa
     4021 accggccggc acgccggcct tccccgagcg gtcccttttt acgccggccc aacccggggc
     4081 cggggaatcc gcccgtcggc gccatttcct taagaatccc tgccacttgc cgataatacc
     4141 aaaagcacga ctgttctctt tttgaccgta acctgaagga gcccggatga cttcgctgcg
     4201 cgatcttctg gaattgatgg tcaaacgcaa tgcttccgat ctgcacctga ccgt
//
LOCUS       DBNGZA010000307         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1541065, whole genome shotgun sequence.
ACCESSION   DBNGZA010000307 DBNGZA010000000
VERSION     DBNGZA010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1541065"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2116
                     /gene="ppdK"
                     /locus_tag="AC16FU_06705"
     CDS             <1..2116
                     /gene="ppdK"
                     /locus_tag="AC16FU_06705"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR2994097.1"
                     /translation="SAYKNLIKRKTGEEFPADPWTQLVQARDAVFKSWNNPRAITYRQ
                     LHGIPGHVGTAATIQAMVFGNLGEGSASGVGFSRNPSTGENVLYGEYLANAQGEDVVA
                     GSRTPKPIAQLAKEIPPAYKQLHEIARRLERHNRDTQDFEFTIQAGKTFMLQTRTAKR
                     TTHAAVRIAVDMVKEKLISKEEALRRIEPEQLNQLLHPRLDPEAEYRPLTKGLAASPG
                     AAAGRVVFSAEEAVKRTRTGEAVILVRNETNPDDIHGMAAAVGILTARGGMTSHAAVV
                     ARGMGKCAVVGAEMIRVFEEKRYFSVGSTVVREKELVTLNGSKGEVIIGSVPTLDPEV
                     TGEFAEFMRWADQTRTLGIRTNADTPADAAVAVKFGAEGIGLCRTEHMFFATDRLPVV
                     QEMIVAAAREDRVKALQKLLPFQKADFKGIFEAMGGRPVTIRTLDPPLHEFLPRKEDL
                     LAQIADLKAKQADPLLIDEKERILKRVEELAEFNPMLGHRGCRLGITYPEITEMQARA
                     IFEAACELVRAGDEVKPEVMIPLVGEVREFLNQKEVVDRVAAETMKKYKVKLKYLVGT
                     MIEVPRACVMAEEIARAADFFSFGTNDLTQMTFGFSRDDAGKFLTGYLEKGILPSDPF
                     VHLDQKGVGQLIDQTVKKGKEVRKDLKVGICGEHGGDPQSIAFCHTLKLDYVSCSPYR
                     VPIARLAAAQAALGAVNGLGTD"
     gene            2353..>2695
                     /locus_tag="AC16FU_06710"
     CDS             2353..>2695
                     /locus_tag="AC16FU_06710"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase"
                     /protein_id="HIR2994098.1"
                     /translation="MDWEKIDRLIALAWEEDVGSGDITTSLFFPPDKTGRAVITAKSE
                     GVLAGAPVVERVFQYARSKTASSAALECQWQKRDGDTLAAGERVAEIAGRLATILAAE
                     RIALNFLGHLSG"
BASE COUNT          572 a          764 c          872 g          487 t
ORIGIN      
        1 ttcggcgtac aagaatttga tcaagcggaa aaccggggag gagttcccgg ccgatccctg
       61 gacgcagctg gtgcaggcca gagatgcggt cttcaagtcg tggaacaacc cccgggcgat
      121 cacctaccgg cagctgcacg gcatccccgg ccacgtgggc accgccgcca cgattcaggc
      181 aatggtgttc gggaacctgg gggaaggttc ggcttcgggc gtcggcttca gccggaatcc
      241 ctccaccggg gaaaacgtcc tttacggtga atacctggcc aacgcccagg gcgaagacgt
      301 ggtggccggc agccggacgc cgaaaccgat tgctcaactg gccaaagaaa ttcccccggc
      361 ctacaagcag ctccacgaga tcgcccgccg gctggaacgg cataaccggg acacccagga
      421 ttttgagttc accatccagg ccgggaagac cttcatgctc cagacccgca cggccaagcg
      481 caccacccac gccgccgtcc ggatcgcagt cgatatggtc aaagagaaac tcatttctaa
      541 agaagaggcg ctgcggcgga ttgagccgga gcagctgaac cagcttttgc acccgcggct
      601 tgatccggag gcggagtacc ggcccctcac caaaggactg gcggcctcgc ccggtgccgc
      661 ggccggccgg gtggtctttt cggccgagga ggcggtgaag cggacccgaa ccggcgaagc
      721 ggtgattttg gtccgcaacg aaaccaaccc ggacgacatc cacgggatgg cggcggcggt
      781 cggcattctc accgcccggg gcgggatgac gagccacgcg gcggtggtgg cccgggggat
      841 ggggaagtgc gcggtggtcg gcgccgaaat gatccgggtc tttgaagaaa aacggtactt
      901 ctccgtgggc agcaccgtgg tgcgggaaaa ggaactggtc accctcaacg gttccaaagg
      961 cgaggtgatc atcgggtcgg ttccgactct ggatccggaa gtgacgggcg aattcgccga
     1021 gttcatgcgc tgggccgatc agacccggac tttgggcatc cgcaccaatg ccgatacgcc
     1081 ggccgatgca gcggtggcgg tcaagttcgg cgccgagggg atcgggctct gccggaccga
     1141 gcatatgttc tttgccaccg accggctgcc ggtggtgcag gagatgatcg tggcagcggc
     1201 ccgggaagat cgggtcaagg ccctgcaaaa acttttgcct ttccagaagg ccgacttcaa
     1261 agggatcttc gaagcgatgg gcgggcggcc ggtcaccatc cgcactctcg atccgccgct
     1321 gcacgagttc ctcccccgga aagaagacct cttggcccag atcgccgacc tgaaagccaa
     1381 acaggccgat ccgctgctca ttgacgaaaa agagagaatt ctgaagcggg tggaggaatt
     1441 ggccgagttc aacccgatgc tggggcaccg cggctgccgg ctgggaatca cctatccgga
     1501 aatcaccgaa atgcaggcgc gggccatctt cgaagcggcc tgcgaactgg tccgggcggg
     1561 cgacgaagtc aaacccgagg tgatgattcc cctggtgggc gaggtccgag agtttctgaa
     1621 ccagaaagaa gtggttgacc gggtggccgc cgagacgatg aagaagtaca aagtcaaatt
     1681 aaaatatctg gtcggaacga tgatcgaagt gccgcgcgcc tgcgtgatgg cggaggagat
     1741 cgcccgcgcc gccgatttct tctccttcgg caccaacgac ctgactcaga tgaccttcgg
     1801 tttctcgcgc gacgacgccg gaaaatttct caccgggtac ctcgagaaag gaattttgcc
     1861 ctccgatccg ttcgtccatc tcgatcaaaa gggcgtggga cagttgatcg atcaaaccgt
     1921 gaagaagggc aaagaagtcc gcaaagactt gaaagtcggc atttgcggtg aacacggtgg
     1981 agacccccag tcgatcgcct tctgccacac gcttaaactc gactacgtct cctgctcgcc
     2041 gtaccgcgtg cccatcgccc ggctggcagc ggcgcaggcg gccctggggg ctgtcaacgg
     2101 attgggaacg gattaaaacg gattagtgcg ccgaagctgg aagcctgacg aacgaacccg
     2161 aggcaggtcg atacgactct atcgagatgc ctcggccatg gagggggaag aacgaattat
     2221 ggagggtgaa ggggatctgc gggcgcggtc aggaaaccgg ggcgaggggt ttttatttga
     2281 ttttttgcgg gtgtcttttc atctttctcc ataggtcagg catcttgcct gacttatatt
     2341 tttcccgtgc aaatggactg ggaaaagatt gaccgtctaa ttgcgctggc gtgggaggag
     2401 gatgtcgggt ccggcgacat tacgacttct ctattctttc cgccggataa aacaggccgg
     2461 gcggtcatca ccgccaaatc tgagggcgtt ctggccggag caccggtggt ggagcgcgtt
     2521 ttccagtacg ccaggtcaaa aaccgcttct tctgctgcgc tcgaatgcca gtggcaaaaa
     2581 agggatggcg atacgttggc tgccggtgaa cgagttgctg agattgctgg tcgattggca
     2641 acgattctgg cggccgagcg gattgcgctc aacttcttag gacacctttc gggca
//
LOCUS       DBNGZA010000308         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1649657, whole genome shotgun sequence.
ACCESSION   DBNGZA010000308 DBNGZA010000000
VERSION     DBNGZA010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1649657"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC16FU_06715"
     CDS             <1..599
                     /locus_tag="AC16FU_06715"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871590.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR2994099.1"
                     /translation="FRWVALSGKPEEIQTTDAVVLKEFAKNEALVRWIRLAQKKVKFQ
                     GLPARICWLGYGERARFGAVINNLVKKKKLSAPVVIGRDHLDTGSVASPYRETEGMKD
                     GSDAIADWPILNGLLNAVSGASWVSVHHGGGVGIGLSIHAGQVVVADGTPQMAKRIER
                     VLTNDPLSGIFRHVDAGYEEAKQVARKFRIKIPMERRQ"
     gene            627..>1570
                     /gene="hutI"
                     /locus_tag="AC16FU_06720"
     CDS             627..>1570
                     /gene="hutI"
                     /locus_tag="AC16FU_06720"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR2994100.1"
                     /translation="MAAELVITDIGQLLTLYSPVPAPRRGKAMADLGLIRNAAVAVTN
                     GRIAAVGTKQTLLKKISRKTKVISAGGRLVTPGLVDPHTHAVFAGNRAGEFEQRLAGQ
                     TYQEIAAAGGGIQATVRATRRASERELETRLLYHLTGMLSWGTTTVEVKSGYGLSLTS
                     ELKLLNVIKRAQKKTPLDLVPTFLGAHTVPREFRHNRKEYVKLLCERMLPAITRHKVA
                     CFSDVFTERGAFSLAESKTIQRTARSLGFALKFHVDELSPLGGAELAAEMGATSADHL
                     EFVSERGIRALARSGTVAVLLPSVSLFLGLKRWAPARR"
BASE COUNT          342 a          410 c          513 g          305 t
ORIGIN      
        1 cgttccgctg ggtggcgctc tccggcaaac cggaagaaat tcaaacgacc gatgcggtcg
       61 tcctgaagga attcgccaaa aacgaagcgc tcgtgcgctg gatccgtctg gcacaaaaaa
      121 aagtaaaatt tcagggactg ccggcgcgga tttgctggct ggggtacggg gagcgggcgc
      181 gcttcggcgc ggtcatcaat aacctcgtca aaaagaaaaa attaagcgcg ccggtggtga
      241 tcggacgcga tcacttggat accggctcgg tggcctcgcc ctaccgcgag accgagggga
      301 tgaaagacgg ctcggacgcc attgccgact ggccgatttt gaacggcctt ttgaatgcgg
      361 tctccggggc gtcgtgggtt tcggtccatc acgggggcgg ggtggggatc gggctttcga
      421 tccacgccgg gcaggtggtg gtggccgacg gcacgccgca aatggcgaaa cgcatcgagc
      481 gcgtgctgac caacgatccg ctctcgggca ttttccgcca cgtcgatgca gggtacgaag
      541 aagcgaagca agtagcccgt aagttccgca tcaaaattcc gatggaacgc cgccagtgag
      601 gaggagacgg tggcgggtgc gtcgagatgg cggctgagct cgtcatcacc gacatcggtc
      661 agcttctgac gctttattcg cccgttccgg cgccgcgcag ggggaaggcg atggcggatc
      721 tcggcctgat tcgcaatgcg gcagtggccg tcactaacgg gcgaattgca gcggtcggga
      781 cgaaacagac actccttaaa aaaatctcaa gaaaaaccaa agttatatca gcaggggggc
      841 ggctggtaac gcccggtttg gttgacccgc acacgcacgc ggtgtttgcc ggaaaccgcg
      901 ccggtgaatt tgaacagcgg ctggcgggcc agacctacca agaaattgcc gcagcaggcg
      961 ggggcattca agcgacagtg cgcgccacga ggagagcttc cgagcgagaa ctggaaacac
     1021 ggcttttata tcatttgacc ggaatgctct cttggggcac gacgaccgtg gaagtgaaaa
     1081 gcgggtacgg gctgtcgcta acatcggaac tgaaattgct taacgtaatc aagcgagcgc
     1141 agaagaaaac acctcttgat cttgttccga cttttttggg cgcgcatacg gttccgagag
     1201 agtttagaca caaccgaaaa gagtatgtca aactcctctg tgaacggatg ctgccggcca
     1261 tcaccagaca caaggtggcg tgcttttcgg atgttttcac tgaacggggc gccttcagtc
     1321 tggctgagtc gaaaacaatt cagcgcacgg cccggagttt aggttttgca ctgaagtttc
     1381 acgttgatga actttctcca ctcggcgggg cggaactggc ggcggaaatg ggggccacgt
     1441 cggcggatca cttggagttt gtttccgagc gcggcatccg ggcgctggcg agaagcggaa
     1501 ccgtagcggt gctcttgcct tcggtttcgc tttttctggg cttaaaacgg tgggcgccgg
     1561 cgcggcggtt
//
LOCUS       DBNGZA010000309         6834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2178777, whole genome shotgun sequence.
ACCESSION   DBNGZA010000309 DBNGZA010000000
VERSION     DBNGZA010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2178777"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..675
                     /gene="mraZ"
                     /locus_tag="AC16FU_06725"
     CDS             202..675
                     /gene="mraZ"
                     /locus_tag="AC16FU_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR2994101.1"
                     /translation="MTAFTGTFRFTLDHKGRVAIPAKFRRRAHPPRRGKGPKARDGSG
                     EQFLLAVGFDGCLALYPPEEWQRVEEKLSGLSFTHRDFRYVNRLLHSRAAEVATDAQG
                     RITIPQEMLAATGIDKEVVIIGASRWVEIWNPEKYQSYVASFGRTFEEVAEQLFT"
     gene            686..1612
                     /gene="rsmH"
                     /locus_tag="AC16FU_06730"
     CDS             686..1612
                     /gene="rsmH"
                     /locus_tag="AC16FU_06730"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR2994102.1"
                     /translation="MSDFHVPVLVGEAVRYLVTDPNGIYCDCTVGGGGHARAVLAQLS
                     PRGRLIGLDRDREALAQIDRTAMEMRDRVTLVHCTFARLKDAVNQEGVTGLHGCIFDF
                     GVSLRQLEDERRGFSYLRDGPLDMRMDATQPKTAATVINQYSDKALALLFWHNADLSI
                     ARRLARLIVQARNQARITTTGQLCAAVLPVLPIRQRPGYLSRIFQAVRMEVNEEINEL
                     QQGLAQALDLLVPGGRLVTIAYHSGEDRQVKHLFQEKARGCICPPRFPRCQCGRKPTL
                     KILTRKAVVPAPEELARNPRARSAKLRAAEKI"
     gene            1681..2040
                     /locus_tag="AC16FU_06735"
     CDS             1681..2040
                     /locus_tag="AC16FU_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994103.1"
                     /translation="MAGLFGSLLVAERMWVFRLAAQNEKLKQDLSRTQTEQSTYQEGV
                     ARLSIYRAIASRAEKEWGLFPPALERLVFVTPQRGLREDSPARSDGVPVAPDRFSVFW
                     SPSAVTSPLPRREETLR"
     gene            2040..4136
                     /locus_tag="AC16FU_06740"
     CDS             2040..4136
                     /locus_tag="AC16FU_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR2994104.1"
                     /translation="MKIERSVNLSAAASGVRLTRRRLKVLLFGSLFIWSLFVLRLVQI
                     QVLQGDRLRRAAFDQDRQKQPLIPKRGEILDRQGRVLARDIEAQSFFAVPESVGSAGE
                     LAALFAPAARCSPFELRRELDNRKNFVWLARQVEAPEVERLVLTRHPGVYQKKELKRD
                     YPLSPLAAGLLGFVDIDRRGLAGVEHSYDSLLTGLFGSRALERDAHGQRYFLDDRTFV
                     PPVNGRTIVLTIDCDLQAILEDELKQGLREARASGATGILLDPRTGEVLALAYLEGRN
                     GPGTKPPAKNRAVADCYEPGSTFKIVTAAAALEEGVKKPEGQIFAENGAWRLGRRVIH
                     DAEKHGWLSFREAVVHSSNIALAKVALEVGGPISYRYAHDFGFGEKTKVDLPGEGRGY
                     LPPQKIWETSQLANFAFGQGVSVNALQLVMAYACIANDGLLLRPYVVKEIGEDGRDTG
                     EKRLPQVVRQVVSVATARTLCQWLAEAVRDGTGRAATVEGLEVAGKTGTAEKPNLERG
                     GYFKNRYVSSFVGFFPADQPRVVGFVAIDDPYGLHFGGQVAAPIFQRVAQRVLPLLPA
                     PPPQLIQPSLVENSGSDAGASLSVNQQPAREENGEPFFAAQAARAEEAAPKITTPASL
                     IGPPVPEDLAPSATVPDLAGLTIREAVGVLGRFGFRARVRGSGVVVAQSPAPGPVRGN
                     TKTVIITGLPPVPRAW"
     gene            4148..5599
                     /locus_tag="AC16FU_06745"
     CDS             4148..5599
                     /locus_tag="AC16FU_06745"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR2994105.1"
                     /translation="MISALEEKEVRGDVRVAISGLEYDSRNIKRDNLFVALSGFALDG
                     HRFAPEAVARGASSLVVERWLNTLPRPQVRVPNSRAALARLAHQFYGHPGEKLTVIGV
                     TGTNGKTTTAYLVKKILETAGEKVGLIGTIQYEIAGEALPAWNTTPESLDLARFFVRL
                     QEAGAKSCVMEVSSHALALHRVEAIDFRVAVFTNLTQDHLDYHRTMENYREAKAKLFR
                     SLQGKDRWAAINRDDPESAYFEQQSGGSVFTYSLEGRGADLIATRIQPSENGSRFELK
                     TDAGTYAVNFPLVGRFNVANALAAAAGAHLVGIGWETVAVGLEKIEPVPGRVEPVQSG
                     QPFTVVIDYAHTPDGLKQILTLARSQSKAGVIVVFGCGGDRDRTKRPLMGEIAGALAD
                     RVIVTSDNPRDEDPQKIIDEVLPGLRGKKNVRTSVDRAEAIADALHAARRGDWVVIAG
                     KGHEEYQIIGKEKIPFSDREEVKRVLRTMGFRG"
     gene            5605..>6834
                     /locus_tag="AC16FU_06750"
     CDS             5605..>6834
                     /locus_tag="AC16FU_06750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR2994106.1"
                     /translation="MMEVISLAPVQTMFPITLDDVMAATGGRLLLPGLNGTAGITGVS
                     TDSRTLKPGELFVALPGERFDGHTFIGEAVGRQAGALLVQHDHEAHLHLASLSLPVLS
                     VPSTLAALGDLGAWFRRKFPLPVVAVTGSVGKTTTKEMSACVLETKYRVHRSAGNYNN
                     LVGIPLSLFALKPDHQVSVLELGMSTPGEIGRLTEITRPEAGVITNIAPAHLETMGSL
                     ENIARAKFELLDGLGETKTVILNADDPFLAARARQERRPVVTFGIQNPADIWAGDRAP
                     DENGCYRFRVNDQTDVRLAVLGLGNVYNALAALAVATRFGITFEKAAKALENYRPAKM
                     RLEVFTVAGVKIIDDSYNANPYSLRCALETLGQMNPPKRDSGRRVAVLADMLELGEKQ
                     ETLHREVGSVAAKAGVDF"
BASE COUNT         1353 a         1985 c         2172 g         1324 t
ORIGIN      
        1 ttcagtcggg ctgttagcaa aggtgaactg aacttgcagg ggaaaagagc gctcggctcc
       61 ccaaaactga gggggaaaac caacaaccga agattttttc cctcacctgc atttttttct
      121 tgacaacctt taggcgaggg gctacattga acccactttt gtgggtaata gtggggaatt
      181 gtggtgattt tgggctgagt tatgacagct tttaccggaa cgtttcgctt tactttggac
      241 cacaaaggcc gggtggccat ccccgccaag tttcgccggc gagcccatcc accgcgaaga
      301 ggaaaagggc cgaaagccag ggatggctcc ggcgaacaat ttctgctcgc cgtgggtttc
      361 gacgggtgtc tggcactcta cccgccggag gagtggcagc gggtggaaga aaaactttcc
      421 ggtctctcct ttacacaccg cgactttcgg tacgtaaacc ggcttctgca cagccgggcg
      481 gctgaagtag ccaccgacgc acagggacgg atcactattc cgcaggagat gctggcggcg
      541 accgggattg acaaggaagt ggtgattatc ggcgcttcgc gctgggtgga aatctggaat
      601 ccggagaagt accaaagcta cgtcgccagt ttcggacgaa cattcgaaga agtagcagaa
      661 cagcttttta cgtaacctat gcgggatgag cgactttcac gttccggtgt tggtcgggga
      721 agcggtccgg tacttggtga ccgatccgaa cgggatttac tgcgactgta cggtcggtgg
      781 gggtgggcat gcgcgtgccg tgctcgcaca actgagtccc cgggggcgac tgattggact
      841 cgaccgggac cgggaggccc tggcgcagat cgaccgaacg gcaatggaaa tgcgcgaccg
      901 ggtgacgctg gtacactgca ccttcgcccg tctcaaagac gcggtaaacc aagagggcgt
      961 caccgggttg cacggctgca tcttcgattt tggcgtgagt ttgcggcagt tagaggatga
     1021 gagacgaggc ttcagctacc tgagggacgg acctctggat atgcggatgg acgcaaccca
     1081 acccaaaact gcggcgacag tgatcaacca gtatagtgac aaggcgctcg ccctcctctt
     1141 ctggcacaat gccgacctgt cgatcgcccg gcgcctggcc cggctcattg tccaggcgcg
     1201 caaccaggca cgcatcacca ctacggggca actgtgtgcc gcggtcctgc cggttctgcc
     1261 gattcgccag cggccgggct atctttcgcg cattttccag gcggtgcgga tggaagtgaa
     1321 tgaggaaatc aatgagttgc agcaggggct ggctcaagca cttgatcttt tggttccggg
     1381 ggggcgcctg gtcaccattg cctaccattc cggtgaagac cggcaggtca aacatctgtt
     1441 ccaggagaaa gcccgcgggt gcatctgccc gccgcgtttc ccccgctgtc agtgcgggcg
     1501 gaaaccgaca ttgaaaattt tgacgcgcaa agcagtggtg ccagcgcccg aagagctggc
     1561 ccgcaatccc cgggcccgga gcgccaaact gcgggcggcg gagaaaatct gacgagtctt
     1621 atgacgacgc ccgcgggcgc cagagtgccg gtcatccgct ttcccgtggc catctgactt
     1681 ttggcgggac tgttcggcag ccttttggtg gccgagcgga tgtgggtgtt tcgtctggcg
     1741 gcgcagaacg aaaaattaaa acaggatctc agccgaaccc aaacagagca gagcacctat
     1801 caggaggggg tcgcccggct ttccatttac cgtgccattg cctctcgggc ggaaaaagaa
     1861 tggggtcttt ttccgcccgc tctggaacgt ttagtcttcg tcacgcccca aagagggctg
     1921 cgcgaagatt ccccggcgcg ctccgacgga gtgcccgtgg cacccgaccg tttttcggtt
     1981 ttctggtccc catctgccgt aacctcaccc cttccccggc gagaggaaac ccttcgctga
     2041 tgaaaatcga gcggagcgtt aacctgtcgg cggccgcgag cggcgtgcgc ctgacgcggc
     2101 ggcggctgaa ggtattgctc ttcggctcgc ttttcatctg gagtcttttt gttttgcgcc
     2161 tggtgcaaat ccaggttttg cagggcgacc ggttgcgccg ggccgccttc gatcaagacc
     2221 gtcaaaagca gcccttgatt cccaaacggg gagaaattct tgaccgtcag ggtcgcgtgc
     2281 tggcccgaga catcgaggcc cagtcgtttt tcgccgtgcc ggaaagcgtc ggcagtgccg
     2341 gggaactggc cgctcttttt gccccggcgg cccgctgctc ccctttcgaa ctccggcgag
     2401 aactggacaa ccgcaaaaac ttcgtttggc tggcccgcca ggtggaagcc ccggaggtcg
     2461 aacggcttgt cctcactcgc caccccggtg tgtaccagaa aaaagaactc aagcgcgact
     2521 accctttaag ccccttggcg gccggattgc tcggcttcgt cgacattgac cggcgcggcc
     2581 tggccggtgt cgaacactcc tacgactccc ttttgaccgg tcttttcggt tcccgcgccc
     2641 tggagcggga tgcccacgga cagcgctact ttttggatga ccgcaccttc gttccgccgg
     2701 tgaacggccg gaccatcgtg ctcacgatcg actgcgacct gcaggcgatc ttagaagatg
     2761 aactgaaaca ggggctgcgg gaagccagag cgtcgggcgc caccggcatt ctcctcgatc
     2821 cgcgcaccgg tgaagtgctg gccctggcct atcttgaagg gagaaacggt ccaggaacca
     2881 agccgccggc gaagaaccgg gcggtggccg actgctacga accgggatcg acgtttaaga
     2941 tcgtaacggc ggcggccgct ttagaagaag gagtgaaaaa acccgaggga cagatctttg
     3001 ccgaaaatgg cgcctggcgg ctcgggcggc gggtgattca cgacgccgaa aagcacggat
     3061 ggctctcgtt ccgcgaggcg gtggtgcact cctccaacat tgccctggcc aaggtggcgc
     3121 tggaggtggg cgggccgatc tcttaccgct acgcccacga cttcggtttc ggcgaaaaaa
     3181 cgaaagtgga cctgcccggc gaaggccggg gctacctgcc gccgcaaaaa atctgggaaa
     3241 cgtcgcaatt ggccaatttt gcttttgggc agggggtgtc ggtcaacgcg ctgcaattgg
     3301 taatggccta cgcctgcatc gctaatgacg gcctgctcct gcgtccgtac gtcgtcaagg
     3361 aaattgggga agatggccgg gacaccggcg aaaaacgcct gccccaggtg gtgcgtcagg
     3421 tggtgagtgt ggcgacggcc cgaaccctgt gccagtggct ggccgaagcg gtgcgggacg
     3481 gcaccgggcg ggccgccacg gtcgaagggc tggaggtcgc cggcaagacc ggcacggcgg
     3541 aaaaaccgaa tttagagcgg ggcgggtatt tcaagaaccg ctacgtttct tccttcgtcg
     3601 gtttcttccc ggccgaccag ccgcgggtgg tcggcttcgt agccatagat gacccgtacg
     3661 ggctgcactt tggcggccag gtggctgcgc cgatcttcca gcgggtagcc cagcgcgtgc
     3721 tgccgctttt gcccgctccg ccgccgcagc tcattcagcc ctccctggtc gaaaattcgg
     3781 ggtccgacgc aggggcctcc ctctccgtca atcagcaacc ggcgagggaa gagaacggag
     3841 agccgttctt tgcggctcaa gcggcccggg cggaagaggc cgctccaaaa ataacgacgc
     3901 cggcgtccct catcggccct cccgtgccag aggatctggc cccatccgca accgttccgg
     3961 atctggccgg cctgacgatc cgggaggcgg ttggcgttct cggcaggttc gggttccggg
     4021 cgcgggtgcg cggttccgga gtggtcgtgg cgcaatcgcc ggcaccgggg ccggtacgcg
     4081 gaaatacgaa aacggtgatc atcaccggct tgccgccggt acctcgggca tggtgacttt
     4141 ggctgagatg ataagcgctc ttgaggaaaa agaagttcgc ggtgacgttc gggtcgcgat
     4201 ttcgggtctg gagtacgact cgcggaatat aaagagggat aatctctttg tggcgttgtc
     4261 cgggttcgct ctcgacgggc atcgttttgc tcccgaggcg gtcgctcgcg gcgcttccag
     4321 tttggtcgtg gagcgctggc tgaatacgct tccccgtccc caagtgcggg tgcccaacag
     4381 ccgcgcggcc ttagcccgtc tcgcacatca gttttacggc caccccggcg aaaaactcac
     4441 cgtgatcggt gtcaccggta cgaacggcaa gaccaccaca gcctatctcg tcaaaaaaat
     4501 attagaaacc gcgggggaga aagttggtct gatcgggacg attcaatacg aaattgccgg
     4561 cgaagccctt ccggcgtgga ataccacgcc ggagtcttta gacctggccc gcttcttcgt
     4621 ccgacttcag gaggccggcg caaaaagctg cgtgatggag gtttcctccc acgccctcgc
     4681 cctccaccgg gtggaggcca tcgacttccg ggtagcggtc ttcacgaatt tgactcaaga
     4741 tcacctcgac taccaccgga cgatggaaaa ctaccgggag gcgaaagcga aactcttccg
     4801 ctccctgcag gggaaagatc gctgggcggc gatcaatcgc gatgaccctg aatcagccta
     4861 ctttgagcaa cagtcaggcg gttcggtctt cacctactcc ctggagggtc ggggggccga
     4921 cctcatcgcc acccggatac aacccagtga aaacggcagc cgctttgaac tcaaaaccga
     4981 tgcgggcaca tatgcggtga attttcctct ggtcgggcgc ttcaatgtgg cgaatgccct
     5041 ggccgccgca gccggtgcgc atctcgtcgg catcggctgg gaaacggtgg cagtcgggtt
     5101 ggagaaaatc gaacctgtgc ccgggcgggt ggagccggtg cagtcaggcc agccctttac
     5161 cgtcgtgatc gactacgccc atacgccgga cggcctgaag cagattctaa cgttagcccg
     5221 cagtcaatcc aaagccggag tgattgtggt cttcggctgc ggcggggacc gcgaccgcac
     5281 caaaagacct ttgatggggg agatcgccgg tgcgttagcc gatcgggtga tcgtcacttc
     5341 ggataacccg cgggacgagg atccgcaaaa aattattgac gaagttttac ccggcctgcg
     5401 cgggaagaag aacgtgcgta cctccgttga ccgggccgaa gcgattgccg acgcgctcca
     5461 cgccgcccgg cgcggagatt gggtcgtcat cgccgggaaa gggcatgaag aatatcagat
     5521 catcgggaaa gagaaaattc ccttcagcga ccgggaagaa gtcaagcgag ttctgaggac
     5581 catgggtttt cgtggataag cgaaatgatg gaagtaatct ctttggctcc ggtacagaca
     5641 atgtttccca tcaccctgga tgacgtcatg gcggccacgg ggggcagact gctcctcccc
     5701 gggttgaatg gcaccgccgg gattacgggc gtttccaccg attcgcgcac cctgaaaccg
     5761 ggtgagctgt ttgtcgcttt acccggagaa cggtttgacg gacacacctt catcggcgag
     5821 gcggttggac ggcaggcagg tgcgcttttg gtccagcacg accacgaggc gcacctgcat
     5881 ctggcttccc tctcgctccc cgtcctttcc gtaccttcca ccctggcggc cctgggcgat
     5941 ctgggggctt ggttccggcg gaaatttccc ctgccggtgg tggcggtgac cggttcggtc
     6001 ggcaagacga cgaccaaaga gatgagtgcc tgtgtactgg aaacgaagta ccgcgtccac
     6061 cggtcggcgg gcaactataa caatctggtg ggcattccgc tgtccctttt tgcactcaag
     6121 ccggatcacc aggtgtcggt gctcgaattg ggaatgagca ctcccggaga aattgggcgg
     6181 ttgaccgaga taacccgccc ggaagcgggc gtcattacca atatcgcacc cgcgcatctg
     6241 gaaacgatgg ggtcgctgga gaatatcgcc agggcaaagt tcgaactgtt ggacggcttg
     6301 ggggagacca aaaccgtcat cctcaatgcc gacgaccctt tcttggcggc gcgagccaga
     6361 caggagcggc ggccagtggt gaccttcggc atccagaacc ccgctgatat ttgggccgga
     6421 gatcgcgcac ccgatgagaa tggttgctac cgtttccggg tcaacgatca aacggacgtg
     6481 cgcttggcgg tgctcgggtt gggaaatgtc tacaatgcct tagcggcgtt ggccgtcgct
     6541 acacgcttcg ggatcacgtt tgagaaggca gccaaggccc tcgagaacta ccggccagcc
     6601 aaaatgcgcc tggaggtttt caccgttgcc ggggtgaaaa tcatcgacga cagttacaac
     6661 gccaatccct attcgctgcg gtgtgcactg gaaactttgg gccagatgaa tccgccgaaa
     6721 cgagactccg gccgccgggt ggcggtgctg gcggatatgt tagagttggg ggaaaagcag
     6781 gaaacgctgc accgggaagt gggcagcgtc gcggccaaag cgggagtcga tttc
//
LOCUS       DBNGZA010000310         6621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2708930, whole genome shotgun sequence.
ACCESSION   DBNGZA010000310 DBNGZA010000000
VERSION     DBNGZA010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2708930"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16FU_06755"
     CDS             <1..291
                     /locus_tag="AC16FU_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR2994107.1"
                     /translation="ADFHLTHRSLGSLAYLATFGSVFAFATYYWLLKRVEATKLSLIA
                     FVTPVVALIVGALFGDEHLSAKIASGSALVVLGIAIATQAKIFSKNQPERWW"
     gene            complement(390..3008)
                     /locus_tag="AC16FU_06760"
     CDS             complement(390..3008)
                     /locus_tag="AC16FU_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR2994108.1"
                     /translation="MIVTIEPSQKDFWEGKWLGPTHGSTSLTTGPSSPVKIGLALSGG
                     GARGFAQIGVLQVLEREGINVNCIAGTSIGGVIGGLYAAGYRAAELERLAETIDWSEL
                     FTNQPKRSSLFVSQKEEADRHLIAVRFDGVRPSLPVALTAGQKLSDLLASLTYRANYR
                     AGGNFDRLPIPYRTVATDLVTGEKVLFDRGDLWEAMRATIAIPLAISPLEKGQRVFVD
                     GGLVDPVPVDVAREMGAELVIAVSTVSDLAPADRLQDPIDIANQVTTVMSAHLRVEQL
                     ARADVVVAPVLGDLANVGFNRIGAAIQAGREAAEQVLPWLREEIRKRKQALRDEKFSV
                     RFVRLEGSGASSAEKFSHLLFSPYPETLSREEIQAKIDSLFASGLFRTARAELHTASD
                     TDFALVYHLEANPTMRAVYFTGNSIFSEAELQACAMIPPTGDLRLHDLRQAAKSVDSL
                     YRSRGYDLAGVQSITYDTSGAVQLVIEEGRIEKITVTGNSHTRRWVVTGDFPLKAGMA
                     FNGNLAARGVANLYASNLFERVGMNLTAGRSGPVVELNVKEKKFVVLRVGGRYNDDYH
                     TEVYGEAGHTNLFGIGNEADLVGHYGSRKWLAGGRFQADQIFRTMYTYRLGFSAGIER
                     RYRYLAHEKVATFDDRRIGITFSLGRHLARLGRLSLDGKWERVQAHFLDQKPDLKYFS
                     GTLAFRSTVDTRDRTPFPTRGNHHEVRFTYAGGPLGSDVRYRSAAGFFENFFPLARRT
                     VINSRLALGFSGSAIPLPDQFRLGGAIAPLAGFASGGTAAPLGGAFRDEFTGPQLFAA
                     SLGLRHKMGDRLYLGARGDLGNVWEKTGQMRFSDLRRGALLSGAVGTPVGPLEVDLGF
                     TQRQKPRLYFFWGHQF"
     gene            3221..4009
                     /locus_tag="AC16FU_06765"
     CDS             3221..4009
                     /locus_tag="AC16FU_06765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR2994109.1"
                     /translation="MPSPKPLRELSFLATKKGRAKRKSFLVEGVRALQELARSDWPVK
                     NIFVQANWEKDVSLAALVAAFRARKIPLYPLTRRELEKITQTETPPGIAAEVAFRRFG
                     LNDLLSRSPRLLVALDGIRDPGNLGTIIRTADAFGAGGLLLSEDTAEFYSPKVVRATA
                     GSLFHLPIAVDLDLPPAFIRLKQAGYLIAVTHRQSSETAAQLSPTGRVCLVLGSEAFG
                     VRKEIASLANHHLSIPITPHVDSLNVAVAAGILLYLLSNRNISP"
     gene            4223..>6621
                     /gene="sppA"
                     /locus_tag="AC16FU_06770"
     CDS             4223..>6621
                     /gene="sppA"
                     /locus_tag="AC16FU_06770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR2994110.1"
                     /translation="MKKRNLCLLASLMIITIKTASFAQMPAIPPFLLPPLSPAVVADP
                     LSLSINPAGLAFTDPALLFLHANSDSTFKGDNAFFLATHQVGFGIEWLGRNFPADGKR
                     YHLGFGGKLGRGVSFGTTYSWIDADRTAYDKFFTWNFGLTYRQSRYVALALVAQNWNQ
                     HQLFNQKIPRQYTASVAVRPIGERLTIALDGFLKQRQTLDDLTYRVFAAFEPLPGLVL
                     QGSVAKGGDFGLGMRFNLAHLFYGSFQNFADKGGYQSGTNYVGLSAVRFPSFLEPKNN
                     LLIEPLSGDLPEERSKPFLFGPFGENFYDKIERIQKAKTDPTVKALLLRIGGLNAGFG
                     KLQELRQAIFDFKSSGKPVAAYLDQPDNRTYYLATAADLIVIPPVSELELIGLKAEVT
                     FVKHTLAKLGIVAEMEHIGEYKNASDLLTRTSMSEAHREVVNAMLDDYYAQFTEAIAK
                     GRGKSQSEVAALIDRGPFISIEAQKAGLVDTFLYWDQVEKYLQARWQKSLRKLDAAVY
                     AECLTYEPSWETPPKIAVVVAAGDIVDGKSGNDFLFGKTLGDLSMVAAIDRAKNDPQV
                     KAVVLRIDSPGGSGTASDVIWRELQLVKEKKPLIVSMSDVAASGGYYIAAPGEKIFAS
                     PGTVTGSIGVISGKLNQLGLYEKIGFDKEVVTRGKRADIWGTTRGFNEEERQIVRHQI
                     DEFYKNFIHLVGEGRRVSDAYVDSVGRGRVWSGHQAKERKLIDEYGGLWEAIAEAKKR
                     AGLAVDRKVKVLFLPQPKFAPFGSSGLLARIQSALGGEPLTLKSLFTLDNLFNGQPLY
                     LP"
BASE COUNT         1351 a         2057 c         1891 g         1322 t
ORIGIN      
        1 gccgatttcc atctcaccca ccggtcactc ggctccctgg cctacctggc gacctttggt
       61 tcggtcttcg cctttgcgac ctactactgg ctcctgaagc gggtggaggc gaccaaactc
      121 tccctcatcg ctttcgtgac cccggtagtg gcgttgatcg tgggtgcgct attcggcgac
      181 gagcacctct cggccaaaat cgcctctggc tcggctctgg tggtgctggg cattgctatt
      241 gccacgcagg ccaaaatatt ctcgaaaaat caacccgaac gctggtggtg acgtttcgtt
      301 tttatggaaa taacgcgtcg ttttccggta gggacgaccc gtcaccatac ggccgtagcg
      361 gtcgcccaaa tcggctctgc ggcagcgcct cagaactggt gcccccagaa gaagtagagc
      421 cgcggctttt gtctttgggt aaaacctaaa tcgacttcca gtggtccgac cggagtcccc
      481 accgcgccgg aaagcaaggc gccccgccgg agatcggaaa accgcatctg ccctgttttt
      541 tcccagacat tgcctaagtc gccgcgcgcg ccgaggtaaa gtctgtcgcc cattttgtgc
      601 cgcaggccga gactggctgc gaagagctgc gggcccgtga actcgtcccg gaaagcccca
      661 cccaggggcg ccgccgtgcc gccggaagcg aagccggcta agggtgcaat cgcgcctccc
      721 aaccggaact gatcaggcag agggatcgca ctgccggaaa agcccagcgc cagccgtgaa
      781 ttgatcaccg tccgccgggc caaaggaaag aaattttcga aaaagccggc tgcgctacgg
      841 tagcgtacgt cggaacccaa agggccgccc gcgtaggtga aacggacctc gtggtgattc
      901 ccgcgggtgg gaaaaggcgt gcggtcgcgc gtatccacgg tggagcggaa ggccagcgtt
      961 ccggagaagt acttgaggtc gggcttttgg tccaggaagt gcgcctgcac cctttcccac
     1021 ttgccgtcca gggataaccg tcccaaacgt gccaggtgcc gtcccagaga aaaggtaatg
     1081 ccgatccggc ggtcgtcgaa ggtggcgacc ttctcgtggg cgaggtagcg gtagcggcgc
     1141 tcgatccccg ccgaaaatcc gaggcggtag gtgtacatcg tgcgaaaaat ctggtcggcc
     1201 tggaaccgcc ctccggccag ccattttcgg gaaccgtaat gacccacgag gtcggcctcg
     1261 ttcccgatgc cgaaaaggtt ggtgtgcccc gcctcgccgt acacttccgt gtggtagtcg
     1321 tcgttgtacc gcccgcccac gcgcaaaact acgaattttt tttccttgac atttaattcc
     1381 acaaccggcc cgctccggcc ggcggtcaga ttcatcccca cccgctcgaa cagattggag
     1441 gcgtacagat tcgccactcc ccgggcagcg agattgccgt taaacgccat cccggctttg
     1501 agcgggaaat cgccggtgac cacccagcgc cgcgtgtggc tgttgccggt gacggtgatt
     1561 ttttcaatcc ggccctcttc gatcaccaac tgcactgcgc ccgacgtgtc gtaggtgatg
     1621 gactggacgc ccgccaggtc atatccccgg ctacggtaga gactatcgac gcttttggcg
     1681 gcctgccgca ggtcgtgcaa gcggaggtcg cctgtcggcg gaatcatggc gcacgcttgc
     1741 agttctgctt ccgagaagat cgaattgccg gtaaagtaca cggcgcgcat ggtggggttg
     1801 gcttcgaggt ggtagaccag agcgaaatcg gtgtcgctcg cggtgtggag ttcggcccgc
     1861 gccgtgcgaa acaggccgct ggcgaaaagt gaatcgattt tcgcctgaat ctcttcccgt
     1921 gaaagcgttt ccggataggg ggagaacaaa aggtgggaaa atttttctgc actggacgcg
     1981 ccgctccctt ccagtcggac aaagcggaca gaaaatttct cgtcgcggag tgcctgcttt
     2041 cttttgcgga tctcttcccg aagccagggt agcacctgct ccgccgcttc gcgccccgct
     2101 tggatggctg ccccaatccg gttgaaaccc acgttcgcaa ggtcacccag caccggggcc
     2161 accaccacgt cggctctggc cagctgctcc acgcgcaggt gggccgacat caccgtggtg
     2221 acctgattgg cgatgtcgat cggatcctgc agccggtccg ccggcgccag gtccgaaacg
     2281 gtggagaccg caatcaccaa ctctgccccc atctcccgcg ccacatccac cggcaccgga
     2341 tccaccaagc cgccgtccac gaagacccgc tgccctttct ccaaaggact gatggccaaa
     2401 ggaatcgcaa tggtggcccg catcgcttcc cacaggtcgc cgcggtcaaa gagcactttt
     2461 tctccggtca ccagatcggt ggcgacggtc cggtagggaa tcggcaaccg gtcaaagttt
     2521 cccccggccc ggtagttggc ccggtaggtg agtgacgcca aaaggtccga gagcttctgt
     2581 ccggcggtga gcgccaccgg cagggaggga cgcacgccgt caaagcgtac ggcgattaag
     2641 tgccggtccg cctcctcttt ctggctgacg aagagactgg agcgcttggg ctggttcgtg
     2701 aaaagctcgg accagtcgat cgtttccgcc agccgctcca gttccgccgc ccggtacccc
     2761 gcggcataga gcccgccgat cacgccgccg atcgacgtgc cggcgatgca gttcacgttg
     2821 atcccttccc gctcgagcac ttgcagcacc ccgatttgcg caaagccgcg cgctccgccg
     2881 ccggagagcg ccagcccgat ttttaccggg gaggagggac ctgtggtgag cgaggtcgaa
     2941 ccatgggtgg ggccgagcca tttgccctcc cagaaatctt tctgcgaggg ctcgatggtg
     3001 acgatcatgg ccggctgagc cggaactgca cgggcaaaaa agaaaaatag aatagagtcg
     3061 agaaaaaggg ctgatagccg ctgtttcggt cgaaaatgta aaggccgcat tgaactcgcc
     3121 gtcccgcctg cggcgggact ccggggtaaa actaccctgc ccggcggaaa aggcaaagag
     3181 tttgacgcgc tttgtccgga aattatgttg gggcggcgct atgcccagtc cgaaacctct
     3241 gagagaactc tcttttttgg ccaccaaaaa agggcgcgcc aaaagaaaaa gttttctggt
     3301 ggaaggtgtc cgcgccctgc aggaactggc ccgctccgac tggccggtaa aaaatatttt
     3361 cgtccaagcg aattgggaaa aagacgtatc cctggctgct ctggtggcag cgtttcgcgc
     3421 gcggaaaatt ccgctgtacc cgctcacccg gcgcgagttg gaaaaaatta cgcaaacgga
     3481 aacacctccg ggcattgccg ccgaagtcgc tttccgccgt tttggactta acgatttgct
     3541 ttcccgctct ccccgtctgc tggtcgcgct ggacggcatc cgcgatcccg gcaatctcgg
     3601 cacgattatc cgcactgcgg atgcctttgg cgcgggcgga ctgcttttgt ccgaagacac
     3661 cgcggagttc tattccccca aggttgtccg cgccactgcc ggctccctct tccacctccc
     3721 aattgccgtc gatctggact tacctcccgc atttattcga ttgaaacaag cggggtacct
     3781 tattgccgtt acccacaggc agagtagcga gacggcggcc cagctgtccc cgacgggaag
     3841 agtttgcctg gtgttgggca gcgaagcgtt tggagtccga aaagagattg cctcgctcgc
     3901 caatcaccac ctttcgatcc ctattacgcc gcacgttgat tcactcaacg ttgccgtggc
     3961 cgccggcatc ctgttatatc ttctctccaa tagaaacatt tccccgtagc ggcggttgcc
     4021 aacctcgccg acggcgagag ttcttgaaac gagggagaaa aaacgtacgg caagttgtac
     4081 cggtaggggc gactttactt gtggtgagcc ccgctaacga cgggatcgaa ccagtcgccc
     4141 gtgagcttct tgaaacgaaa gcgagaaaat accgtacgtc agagggagaa cgaggaaggt
     4201 acctcgcaaa ggagtgagcg caatgaaaaa acgtaatctt tgtttgcttg cgtctttaat
     4261 gatcatcacc atcaaaactg cgagctttgc ccagatgccc gcgatccccc ccttcctcct
     4321 gccgcccctt tccccagcgg tggtggctga ccccttgagt ctttcgatca accccgcggg
     4381 gctggccttc accgacccgg cgctcctctt cctccacgcg aacagcgatt ccaccttcaa
     4441 aggggacaac gcctttttcc tcgccacgca tcaggttggc tttggcatcg aatggctcgg
     4501 gcggaacttt cccgccgacg gcaaacgcta ccatctgggc ttcggcggca agctggggcg
     4561 cggcgtctcc ttcggcacca cctactcctg gattgatgct gatcgtaccg cctatgacaa
     4621 gtttttcacc tggaatttcg gcctcaccta ccgccagagc cgctacgtcg ccctcgctct
     4681 ggtggcccag aattggaatc agcaccaact tttcaatcaa aagattcccc ggcagtacac
     4741 cgccagcgtc gccgttcgac ctattggtga gagactgacc attgccctcg acggatttct
     4801 gaaacagcgg cagacgctgg atgacctcac ctaccgcgtc tttgccgcct ttgaaccgct
     4861 ccccggcctc gttctgcagg gatcggtggc caaaggcgga gatttcggcc tcggcatgcg
     4921 tttcaatctg gcgcatcttt tctacggttc gttccagaat tttgcggaca aaggcggtta
     4981 ccagtccggg acgaattacg tcggcctctc cgccgtgcgc ttcccctcat tccttgagcc
     5041 gaagaataat cttttgatcg agcccctctc cggtgacctg ccggaagagc gctcgaaacc
     5101 cttcctcttc gggccattcg gcgaaaattt ctacgacaaa atcgagcgca ttcagaaagc
     5161 caaaaccgac ccgacggtaa aggcactcct cctgcgcatc ggcggcttga acgccggctt
     5221 cgggaaattg caggaactgc ggcaggcgat tttcgatttc aaatcgtccg gcaaaccggt
     5281 ggctgcctat ctggatcagc cggataaccg cacctactac ctggccaccg ccgccgacct
     5341 catcgtcatt ccgccggtga gcgaactgga actgattgga ctgaaagccg aagtcacgtt
     5401 cgtcaagcac accctcgcca aattaggcat cgtggcggaa atggagcaca tcggggagta
     5461 caaaaacgcc tcggatcttt tgacccgcac cagtatgtcg gaggcccacc gggaagtggt
     5521 gaacgccatg ctggacgatt actacgccca gttcaccgaa gcgattgcca aaggtcgcgg
     5581 gaaatcacaa tccgaggttg ccgcgctgat tgaccgcggg ccgttcatct ccattgaagc
     5641 gcagaaagcc gggctcgtcg acaccttcct ctactgggat caggtggaaa aatatctcca
     5701 ggcgcgctgg caaaaatccc tcagaaaact cgatgccgcc gtctatgccg aatgcttgac
     5761 ctacgagccc tcctgggaaa cgccgcccaa gattgccgtt gtcgtcgctg ccggagatat
     5821 cgttgatggc aaaagcggca acgacttcct cttcggcaag accctgggcg acctcagcat
     5881 ggtggctgct atcgaccggg cgaaaaatga tccacaggtc aaggcagtcg tcctgcgcat
     5941 cgacagcccc ggcggctccg gcaccgcgtc ggacgttatc tggcgcgaac tccagttagt
     6001 caaggagaaa aagcccctga tcgtctcaat gtcggatgtg gccgcctccg gcggctacta
     6061 catcgccgcg ccgggcgaga aaatcttcgc ctctccgggg acggtgaccg gctccatcgg
     6121 cgtcatctcc ggcaaactca accagttggg actctatgag aaaatcggtt ttgacaaaga
     6181 agtggtcacc cgcggtaaac gcgccgatat ctgggggacg acgcgcgggt ttaacgaaga
     6241 ggaaaggcaa atcgtccgcc atcaaattga tgaattctac aaaaacttca tccatctggt
     6301 cggagaggga cgccgtgtct cggacgccta cgtcgattcc gtaggtcggg gtagggtctg
     6361 gtccggccat caagcgaaag agcggaagtt gattgacgaa tatggcggac tgtgggaggc
     6421 gattgccgag gccaaaaagc gcgccggatt ggcggttgac cggaaggtga aagtgctctt
     6481 cctgccccag ccaaaatttg ctcccttcgg ctcttccggc ctcctcgcca gaattcagtc
     6541 cgccttgggc ggcgagccgc tcacgctcaa gagtcttttt acattagaca acctcttcaa
     6601 cggccaaccc ctctacctgc c
//
LOCUS       DBNGZA010000311         3463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2921080, whole genome shotgun sequence.
ACCESSION   DBNGZA010000311 DBNGZA010000000
VERSION     DBNGZA010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2921080"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16FU_06775"
     CDS             <1..463
                     /locus_tag="AC16FU_06775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR2994111.1"
                     /translation="PNRMSKFVQSEWSELTYHIIGSAMAVHRDIGPGHKEAIYQRALE
                     QKLREEKLTVSSQKSIEVTNDGTLVGLYFVDLLVEDKIVVELKALNRIENNHVQQVIA
                     YLAATGCPLGLLLNFGTGSLQTRRIFPPKNVSAYLINQQWLRAPEWMRPSE"
     gene            complement(500..1651)
                     /locus_tag="AC16FU_06780"
     CDS             complement(500..1651)
                     /locus_tag="AC16FU_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016742414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HIR2994112.1"
                     /translation="MPIYHSLGQIPHKRHTQFRQPDGALYSEQLMGSKGFSGMYSLLY
                     HSHPPTRVTKIGPVVENKIEFSDKKELKHYHFKTKEIEAGGDPITGRKVFMANEDVSL
                     AIARPTQKMSYFYRNGQADELYFIHEGSGVFQTIFGRLTFRPGDYVIIPRGTTYKIVS
                     NPGPNRFLVIESPSQIETPRRYRNEHGQLLEHSPYCERDIRRPEALETFDEKGEFEVK
                     IKARNRLTTYVFDFHPLDVVGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGHNF
                     VVCSFCPRKFDYHPLAVPIPYNHSNVESDEMLYYCNAEFMSRKDIEYASITLHPSGIP
                     HGPHPGAVESALGKERTNELAVMMDTFRPLSVTKLAEKYEDDKYAYSWL"
     gene            complement(1718..2554)
                     /locus_tag="AC16FU_06785"
     CDS             complement(1718..2554)
                     /locus_tag="AC16FU_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR2994113.1"
                     /translation="MKGGKVYTLFREFISLRQFLEQEQWLRLAREAEKRTTNGWEVEK
                     VLLAPPVPDPKTFRDFYAFEAHVKRARASRGLEMVPEWYELPVFYYSNPTVFVGPEEK
                     VKRPSYTQALDYELEIACIIGKEGKNIKASEAEAYIAGYTILNDWTARDAQRQEMKVG
                     MGPAKGKDFATALGPYLVTPDELQDLKNGDRYDLAMTARVNGKEYSRGNFKDIHWTFP
                     QMIERGSQEVTLFPGDVLGSGTVGTGCIMELGPDKYGWLKPGDVVELEIERLGILRNT
                     VV"
     gene            complement(2611..>3463)
                     /gene="hppD"
                     /locus_tag="AC16FU_06790"
     CDS             complement(2611..>3463)
                     /gene="hppD"
                     /locus_tag="AC16FU_06790"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010610317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR2994114.1"
                     /translation="HGDGAKEVALRVQDAESAFRETTKRGARAVQEPTEYRDDNGVLK
                     KSAIATYGEVIHTFVERKNYAGVFAPGYKKCLQRGKGLGLKEPDHVVGNVELGEMNEW
                     VNFYRDKMGFSQMLHFDDQDISTAFSALMSKVMQNGTGRIKFPINEPAEGKRKSQIQE
                     YLDYYRSPGVQHIALSTDDIVATVTRMRAAGVEFLYVPTTYYDLLQERVGKIDEPVED
                     LKRLGILVDRDDEGYLLQIFTKPVQDRPTLFFEVIQRKGSRGFGKGNFKALFESIERE
                     QAQRGNL"
BASE COUNT          721 a          976 c          925 g          841 t
ORIGIN      
        1 gccaaatcga atgtctaaat ttgtacagag cgaatggtca gaattgacct accatattat
       61 tggttcggcg atggcagtcc accgcgacat cggacccggg cacaaagagg caatttacca
      121 gagagcgtta gagcaaaaac tgcgcgaaga gaaacttact gtgtcttctc aaaaaagcat
      181 tgaggtgaca aatgacggaa ctctggtggg cctttacttt gtcgatctcc tcgttgagga
      241 caagatcgtt gtggaattga aggcgttaaa cagaatcgaa aacaatcacg ttcagcaagt
      301 aattgcttat ctggctgcga ctggatgtcc tttgggtcta cttttgaatt tcggaacagg
      361 gagtttacag acgagacgta ttttcccgcc taaaaatgtt tctgcgtatt tgatcaatca
      421 gcaatggcta cgcgctcctg agtggatgcg accttcggaa taataatccg ttttaatccg
      481 ttcaaaatcc gttgacaggt tacaaccagc tgtacgcata cttgtcgtcc tcatactttt
      541 cggccagctt cgtgacgctc agcggccgga aggtatccat catcaccgct aattcgttgg
      601 tgcgttcctt gcccagggcc gactccaccg cgcccgggtg cggcccgtgc ggaatgccgg
      661 aggggtgcag ggtgatggac gcgtactcga tgtctttccg gctcatgaac tcggcgttgc
      721 agtagtagag catctcgtcc gactccacgt tggagtggtt gtaaggaatg ggaaccgcca
      781 gcgggtggta gtcgaacttg cgcgggcaga aggagcagac cacgaagttg tgtccttcga
      841 aagtctggtg caccggcggc ggctggtgga tgcgtccggt gatcggctca aagtcctcaa
      901 tgttgaaaat ccaggggtag aggtagccgt cccagcccac cacgtctaag gggtggaaat
      961 cgaaaacgta ggtcgtcagc cggttgcggg ctttgatctt tacttcgaac tcgccttttt
     1021 cgtcaaaagt ctccagcgcc tccggccggc ggatgtcccg ctcgcagtag ggagaatgtt
     1081 ccaacagttg cccgtgctca ttccggtagc gccgcggcgt ttcgatctgg ctgggcgatt
     1141 caatgactaa aaaccgattc ggcccggggt tggagacgat tttgtaggtc gtgccgcgcg
     1201 ggatgatgac gtaatcaccc ggacggaagg tgagccgtcc gaaaatggtc tggaaaaccc
     1261 ccgatccttc gtgaatgaag tagagctcgt cggcctgccc attgcggtag aaataactca
     1321 tcttttgcgt cggacgcgct atcgccaggg aaacgtcttc gttcgccata aacaccttgc
     1381 ggcccgtaat gggatcgccg cccgcttcga tttctttggt tttgaagtgg tagtgcttca
     1441 gctctttttt gtcgctgaat tcgattttgt tctcgaccac cgggccgatc ttcgtcaccc
     1501 gcgtgggggg atggctgtgg tacaggagcg aatacattcc cgaaaagccc ttggaaccca
     1561 tcagttgctc ggagtagagc gctccatcgg gctggcggaa ttgcgtgtgt cgcttatggg
     1621 gtatttgacc taaggaatgg tagatgggca tcgaatgctc ctttcaaaac tatccccaat
     1681 ggaagacgcg gcactgaagt gccgcttcct gggattttta gacaacggta tttctgagga
     1741 ttcccaatct ttcaatttcc agttccacca catcccccgg cttgagccag ccgtacttat
     1801 ccgggcccaa ttccataata cagccggtgc cgacggtgcc cgaacccaaa acatcaccgg
     1861 gaaagagggt gacttcttgc gatcctcgct caatcatctg cggaaaagtc cagtgaatgt
     1921 ccttgaagtt gccccgcgaa tattctttgc cgttcacgcg ggccgtcatg gccaaatcgt
     1981 agcggtcacc gtttttgaga tcttgtaatt catccggcgt caccagatac ggccctaacg
     2041 cggtagcgaa atctttccct ttggccgggc ccatgccgac cttcatctcc tgccgttgcg
     2101 catcccgcgc cgtccagtcg ttcaaaatcg tgtagccggc gatataagcc tccgcctcgc
     2161 tcgctttgat gtttttcccc tctttgccga tgatgcaggc aatctccaac tcgtaatcga
     2221 gtgcctgagt gtaggaaggc cttttgactt tttcctccgg cccgacgaaa accgtcggat
     2281 tcgagtagta gaaaaccggg agctcgtacc actccggcac catctccaat ccccggctgg
     2341 cgcgggcgcg cttgacgtgt gcctcgaagg cgtagaagtc ccgaaaggtc ttcggatccg
     2401 gcaccggcgg cgctaaaaga actttttcta cttcccagcc gttggtagtc cgcttttccg
     2461 cctcccgagc cagtcgcagc cattgttcct gctctaaaaa ctgccgcagg gagatgaact
     2521 ccctgaaaag cgtgtagact ttcccgccct tcaccactcc gaccgcgctc tgctccggca
     2581 ggtcgggagg gtagaaggtc accagccgca ctacaggttc cctcgctggg cctgctcccg
     2641 ctcaatggat tcaaacagcg ccttgaaatt tcccttgcca aagccgcgcg agcccttgcg
     2701 ctgaatcacc tcaaagaaaa gcgtcggtct atcctgcacc ggcttggtga aaatctgcag
     2761 gagatatcct tcgtcgtcgc ggtcgactaa aatccccaga cgttttagat cctccaccgg
     2821 ctcgtcgatt ttccccaccc gctcttggag caaatcgtag taggtcgtcg gcacgtagag
     2881 gaactccact ccggccgcgc gcatcctcgt gacggtggcc acaatgtcgt cggtggagag
     2941 ggcgatgtgc tggacgccgg gggagcggta gtagtccaaa tattcctgaa tttgcgactt
     3001 gcgctttccc tccgccggtt cgttgatggg gaacttgatc cgcccggtgc cgttctgcat
     3061 tactttcgac atcaaggccg agaaggcggt tgaaatgtcc tgatcgtcaa agtggagcat
     3121 ctgcgaaaag cccatcttat cgcggtagaa gttcacccat tcattcatct cgcccaattc
     3181 gacgttcccc accacgtggt cgggttcttt tagtccaaga cctttgcccc gctgcagaca
     3241 ctttttgtat cccggtgcga aaactccggc gtaatttttg cgctcgacaa aggtatgaat
     3301 cacctctccg taagtggcaa tggccgattt tttgagcact ccgttatcat cacggtactc
     3361 cgtcggctcc tgcaccgccc gcgctccccg tttagtagtt tcgcggaaag cgctttcggc
     3421 atcctgaact cgcagggcaa cctcctttgc cccgtcgccg tgc
//
LOCUS       DBNGZA010000312         4874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1224777, whole genome shotgun sequence.
ACCESSION   DBNGZA010000312 DBNGZA010000000
VERSION     DBNGZA010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1224777"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC16FU_06795"
     CDS             <1..322
                     /locus_tag="AC16FU_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994115.1"
                     /translation="TEKGRRDSVILITVIANNDAVQRAAVRWAEKQGYSGMGKYDKAS
                     FRIDIIRGFFVEQTLKKIYALQASQSWSRSLWAVKIYFEEGSNSYYSALIENGQISDV
                     IEHN"
     gene            complement(355..1338)
                     /locus_tag="AC16FU_06800"
     CDS             complement(355..1338)
                     /locus_tag="AC16FU_06800"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR2994116.1"
                     /translation="MKISMLETTAVGSYPKPDYLVKARNRFAKGKIPYTELHELELQA
                     TREWIKIQEDVGLDILVHGEQERGDMTTYFAENGRLEGLGISGLVRSYGNRYYRKPVV
                     QGEIKRPRPMTVEMFKYAQGLTKKPVKGMLTGAYTMCDWSFNTYYPTRRDVVLAFAKV
                     LHEEVADLEKAGAKYIQIDEPALSTRPDEVDLAIEAMKIVTAGIKAKTISHICYGDFR
                     VMYPRVLDLPVDVLDLEFANSRFQNLEIFRTPKFTKGVSVGVVDIHSHVIETKEEVKA
                     GIKKALEVFDVDKVWIDPDCGLKTRTVQEAIDKLKVMVEAVGEVKRELGLS"
     gene            complement(1362..1682)
                     /locus_tag="AC16FU_06805"
     CDS             complement(1362..1682)
                     /locus_tag="AC16FU_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR2994117.1"
                     /translation="MWSALRKARDILRQRIDFCVALYRDPRTPRSARVFLWLALGYAA
                     LPFDLIPDFIPVIGHIDDAVIIPVLLLIAFRLIPKEVYQEHHDHFFGKPARFVNPGGC
                     GGIQ"
     gene            complement(1743..2663)
                     /locus_tag="AC16FU_06810"
     CDS             complement(1743..2663)
                     /locus_tag="AC16FU_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2994118.1"
                     /translation="MLTDRVIKTSITIKAPAEDILTAFLDIGWMREWWGIEGGLVEPK
                     RDGLWTVVWGHSAEGYQAIGTGIVRRLGPYLLEIDSNLVFFPQRQILGPRRFQLQCRP
                     EGDKTFVDIEESGFQSGGDWDWFYESVQKGWQEVLPVLKSFLERPHARATLPSAAPVS
                     DRRVGTSIEVAASRERALEAFLNHNDLAQWWGVAKSLIDPKPQGLWALAWEPGEKGYR
                     YVTAGVIGMFDPPERIIIDQLAYFNPEFPILAPMRLGLGFDGDDTRTQISVVQTGYGT
                     GPDWDRYYEAVVQGWPMALARLKEFLERKS"
     gene            complement(2666..3637)
                     /locus_tag="AC16FU_06815"
     CDS             complement(2666..3637)
                     /locus_tag="AC16FU_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994119.1"
                     /translation="MLKTTVVGNYPKISSDRSAPNLRNALNQAESGKITPQQVEEVYQ
                     STIRRVIEEQKRAGVDLITDGQIRWNDIVTPFAKKISGFEINGLLRFFDNNVYYRKPV
                     LKAKPAFNGSAAVEQFRFAQKFAGENLKAVIPGPFTFARMSVDEFFGNEEKFVLTLAE
                     LLHQEAVALVEAGATWVQIDEPCLCVEPGKIDLVRRGIEIVTRGLKAKTMLFLYFGSI
                     KPLVPKLFEFPVDAFGVDCVSRPENFSLLLTAPKGKGICCGCLDGRNIKLETDRELFP
                     YFEKLAARTDGGEVFISPSSGLEFLPHADAVQKLERMVTAVRTFNTR"
     gene            complement(3637..4830)
                     /gene="aroC"
                     /locus_tag="AC16FU_06820"
     CDS             complement(3637..4830)
                     /gene="aroC"
                     /locus_tag="AC16FU_06820"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR2994120.1"
                     /translation="MVRFLTAGESHGQALAGILEGLPAGLGVDISFLNAELARRQKGY
                     GRGGRMQIEKDTLRILSGVRHLKTLGSPIAFLIENRDWENWKEIMAVEDNPDSAAVAD
                     PRSAPRTAPRPGHADLAGAIKYGQKDIRNVLERASARETAARVGAGAFAKLFLREFGI
                     DFASHVVEICGVAVPDGRPAFAEIQSRAPKSELHCIDPATEEKMKAKIDEAIARKDTV
                     GGVFEVIVTGVPIGLGSFVHWDRRLDAQLAAAVMSIPSAKGVEIGMGFAQAHRFGSEV
                     HDQIFFEGKTQSASRQKGFFRRTNNAGGLEGGVTNGEDLVLRAVCKPLSTLMQPLATV
                     DIVTKEPKEAIVERSDVCVVPCYGVVAEAVVALVLANAFLEKFGGDSLVEIRDHFQTY
                     NERKF"
BASE COUNT         1046 a         1457 c         1299 g         1072 t
ORIGIN      
        1 aacagaaaaa gggcgcagag atagtgttat tcttattact gtaattgcta ataatgatgc
       61 agttcaacgg gccgctgtcc gctgggccga aaagcaagga tattctggaa tgggaaagta
      121 tgataaagca tcctttagaa tcgatatcat acgcggtttc tttgtcgagc agaccctgaa
      181 aaagatatac gctttgcagg cgagtcaaag ctggtcccgt tctctttggg ctgtaaaaat
      241 atattttgaa gaagggtcga attcttacta ctcggctcta attgagaatg gacagataag
      301 tgacgtcatt gaacacaatt gaaaagcaag tgtggtaaat ttgggtgtag aggactaact
      361 taaccccagt tccctcttta cctcccccac cgcctcaacc atcactttga gtttgtcgat
      421 cgcctcctgc acagtgcggg ttttgaggcc gcagtccggg tcaatccaga ctttgtccac
      481 gtcaaaaacc tccagcgcct ttttgatccc ggccttcacc tcttctttgg tctcaatgac
      541 gtggctgtga atatccacca cgcccaccga cacgcccttg gtgaacttgg gtgtgcggaa
      601 gatctccaag ttctgaaagc gcgagttggc gaactccaaa tcgagcacat ccaccggcaa
      661 atcgagcacg cgggggtaca tcacccgaaa gtcgccgtag cagatgtgcg aaatagtctt
      721 ggctttgata cccgcggtga cgattttcat tgcttcaatg gccagatcaa cttcatcggg
      781 acgggttgag agcgccggct cgtcaatctg aatgtacttg gcgcccgctt tttcgagatc
      841 ggccacttcc tcgtgcaaga ctttcgcaaa ggccagcacc acgtcccgcc gcgtggggta
      901 gtaggtgttg aacgaccagt cgcacatcgt ataagccccg gtgagcatcc ctttgaccgg
      961 ctttttggtc agcccctgcg cgtatttgaa catttcgacg gtcatcggac gcgggcgctt
     1021 gatttctccc tgcaccaccg gcttgcgata gtagcggttg ccgtaggagc gcaccaagcc
     1081 ggagataccc agtccctcta aacggccatt ctcggcaaag taagtggtca tatcaccccg
     1141 ctcctgctcg ccatgcacca gaatgtccag tccaacatcc tcctggattt tgatccattc
     1201 ccgggttgct tggagttcca attcatgcag ttctgtgtag ggaattttcc ccttcgcaaa
     1261 gcggttgcgc gccttgacga gatagtcggg cttcgggtaa ctgccgacgg cggtcgtttc
     1321 cagcattgaa attttcacct cttttcacct acaccacagt cttactgaat ccctccgcac
     1381 ccccctggat ttacaaaccg cgccggtttt ccgaagaaat ggtcgtggtg ctcctgatag
     1441 acctctttcg gaatcaaccg gaaggcgatc aaaagcagaa ccgggataat tacggcatcg
     1501 tcaatatggc cgatcaccgg aataaaatcg ggaatgaggt caaaaggcaa ggcggcatac
     1561 cccagcgcca gccagaggaa cactcgcgca ctgcggggag tgcgtggatc ccggtacaac
     1621 gccacgcaga aatcaatccg ctggcggagg atgtcgcgtg ccttacgtag cgcactccac
     1681 atgatacctc ttcctcgagc actgacaaaa tgggcctatt gttctggttg actgtagtcc
     1741 aatcaactct tgcgttccaa aaattctttt aaccgcgcca gggccatcgg ccacccttgc
     1801 acgaccgcct cgtagtagcg atcccagtcc gggccggtgc cgtacccggt ctgcaccacc
     1861 gaaatttgcg tgcgggtgtc gtccccgtca aaaccgaggc cgaggcgcat cggcgccaag
     1921 atcggaaatt cggggttgaa gtaggccagc tggtcgatga tgatccgctc cggcggatcg
     1981 aacatcccga taacaccggc agttacgtaa cggtagccct tctcgcccgg ctcccaggcc
     2041 agagcccata gaccctgcgg tttggggtcg atgagacttt tcgccactcc ccaccactgc
     2101 gcgaggtcat tgtggttcaa gaacgcttcc aaagcgcgct cgcggctggc ggcaacctcg
     2161 atagaggtgc cgactcgacg gtcgctgacg ggagcggcgg atggcaaggt cgctcgggcg
     2221 tggggtcttt ccaaaaaact cttcagcacc ggtaagactt cttgccagcc cttttgtact
     2281 gattcgtaaa accaatccca gtcaccgccg gactggaatc cagattcttc gatgtccacg
     2341 aaggtcttat ctccttccgg ccggcactgg agctggaagc gcctcggtcc cagaatctgc
     2401 cgctgtggga agaacaccag attcgagtca atttccagaa ggtagggacc cagccggcga
     2461 acgatgccgg tgccaatggc ctggtagcct tccgccgaat gcccccagac gaccgtccag
     2521 aggccgtcgc gcttcggctc aaccaggccg ccttctatcc cccaccactc ccgcatccag
     2581 ccgatatcca gaaacgcggt gagaatatcc tcggccggcg ccttaatggt gattgaggtt
     2641 ttgatcacgc gatcggtcag catcgtcacc gtgtgttgaa cgtccgaacg gcggtcacca
     2701 tccgctctaa tttctgcacc gcgtcggcgt gcggcagaaa ttcgagtccg ctgctgggtg
     2761 aaatgaatac ttcaccgccg tcggtacgcg ccgccagttt ctcgaaataa ggaaagagtt
     2821 cccgatccgt ctccagtttg atattgcgcc cgtccagaca gccacagcag atccctttcc
     2881 ccttcggcgc tgtaagcaaa agcgaaaaat tctcaggccg tgagacacaa tcgacgccaa
     2941 aggcatcgac ggggaactca aatagtttcg gtactaatgg cttgatgctc ccaaagtaga
     3001 ggaagagcat tgttttggct ttcagaccgc gggtgacgat ttcgatgccc cggcgaacca
     3061 gatcaatctt gccaggttcg acgcagaggc acggctcgtc tatctgaacc caggtcgctc
     3121 cggcctcgac cagcgcgacc gcttcctggt gcaggagttc agccagggtc agaacgaact
     3181 tctcttcatt cccgaaaaac tcatccaccg acatccgcgc aaaggtgaac ggcccgggga
     3241 tgaccgcctt gagattttcg ccagcaaact tctgtgcaaa acggaattgc tccaccgccg
     3301 cggagccatt gaacgccggt ttggctttca gcaccggctt gcggtagtag acgttgttgt
     3361 cgaagaaacg caagaggccg ttaatctcga agccggaaat ttttttggcg aaaggcgtca
     3421 cgatgtcgtt ccagcggatt tgcccgtcgg taatgagatc gacccccgca cgcttttgct
     3481 cctcaatgac ccgccggatg gtactctggt aaacctcttc tacctgctgc ggagtgattt
     3541 tgccgctctc ggcttgattc agcgcgttgc gcagattggg cgcggagcgg tcgctggaaa
     3601 tcttgggata gttgccgacg acggtcgttt tgagcatcag aatttccgtt cgttgtaggt
     3661 ttgaaagtga tcgcgtattt ccaccagact gtcgccgccg aacttttcca agaaggcatt
     3721 cgccaagact aatgcgacga ccgcctccgc cacgacgccg tagcagggca ccacgcagac
     3781 gtcggaacgc tcgacgatgg cttccttcgg ctccttcgtg acgatgtcca ccgtcgccaa
     3841 aggctgcatt aaggtcgaaa gcggtttgca gaccgcgcgc aggactaagt cttcgccgtt
     3901 ggtgacgccg ccctccaagc cgcccgcatt attcgtccgg cgaaagaaac ctttctggcg
     3961 cgacgctgat tgggtctttc cctcaaaaaa aatctggtcg tggacctccg agccgaaacg
     4021 gtgcgcctga gcgaacccca tgccgatctc cacgcctttt gccgaaggga tcgacattac
     4081 cgcggcggcc aactgagcat ccagccggcg gtcccagtga acaaaactcc ccaagccgat
     4141 gggcacgccg gtgacgatca cttcgaacac tccgcccacc gtgtctttgc gcgctatcgc
     4201 ctcgtcgatt ttggctttca ttttttcttc cgtcgccgga tcgatgcagt gtaactcgga
     4261 cttgggtgcc ctggattgga tttcagcgaa cgctggccgt ccgtccggca ccgcaacgcc
     4321 gcagatttcc accacgtggc tggcgaaatc gatgccgaat tcccggagga agagtttggc
     4381 aaaggcaccg gcgccgacgc gagccgccgt ctcacgggct gacgcgcgct ccagcacgtt
     4441 gcggatgtct ttttgcccat acttgatcgc accggccaga tcggcgtgcc ccggacgggg
     4501 agcggtgcgc ggtgcactgc gcgggtcggc gactgcggct gaatccggat tgtcttccac
     4561 cgccataatc tctttccagt tttcccagtc ccggttctcg atcagaaacg cgatcgggct
     4621 gcccagagtt ttcaagtgac ggacgccgga gagaatccgg agcgtatcct tttcgatttg
     4681 catccgtccg ccccggccgt agcccttctg ccgccgcgcc agttcagcat taaggaaaga
     4741 aatatcgacc cccaggcccg ccggcagccc ctcgaggatg cccgctaagg cctgcccgtg
     4801 tgattctcct gccgtcaaaa agcgcaccat cgtctttacc ccgaacaacc caaatgagtt
     4861 tccggaagtg aaag
//
LOCUS       DBNGZA010000313        13739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1754880, whole genome shotgun sequence.
ACCESSION   DBNGZA010000313 DBNGZA010000000
VERSION     DBNGZA010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1754880"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1352
                     /locus_tag="AC16FU_06825"
     CDS             318..1352
                     /locus_tag="AC16FU_06825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2994121.1"
                     /translation="MTLSDLEHLKNLLNEREQNLTAWLDASGSPNGEDVQKAQALLTD
                     IREALGRVENQTFGACQGSAPAGICKGEVELYRLEVQPVTEVCLGCITKEEQARLEEE
                     LFLASKIHRALLPQSIERIEGYDVGVKALAARSVGGDYYDFLPSANGGRLRVVIADTM
                     GKGIPAGLLMSHVQGALKILSEDIESPRLLISRLNQWLCRNVPVTKFVSLSCLALDSR
                     AGRDSQITYTNAGHCPPILVRNDGKVERLEPTGGVLGVHEGFTYEEHTLSLSPGDLLV
                     LYTDGVTEAENYHGGMFGEERLIEFAQTRRTDPLDKFLDDLLGEVQFFSEKPEMDDDF
                     TVIALRKTAS"
     gene            1691..2161
                     /locus_tag="AC16FU_06830"
     CDS             1691..2161
                     /locus_tag="AC16FU_06830"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2994122.1"
                     /translation="MSPQELTAYFDEIYALNLACLDLVPEEKLDFQPVPEVKSLRLLL
                     HHMYVNQKFYLLTARKGTMDVDDYKAMMKQVPDTKESLRREMGETFAEFKQALGDEAL
                     LKKEVQTLSGSRRVYHLFIGELEHQLHHRGQVYTYLRLLGLKPPDSGLYMGLAS"
     gene            2204..2617
                     /locus_tag="AC16FU_06835"
     CDS             2204..2617
                     /locus_tag="AC16FU_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR2994123.1"
                     /translation="MKSHTEYLWFNTKKHREFINITGEVEKALGKSGIKEGFALVSAM
                     HITASVYVNDAESGLIADIEEWLEKLAPSGVNYRHHRTGETNGDAHLKNLLMHHEVIV
                     PVTAGKLDFGPWQQIYYAEFDGQRKKRVVIKIIGD"
     gene            2889..4427
                     /locus_tag="AC16FU_06840"
     CDS             2889..4427
                     /locus_tag="AC16FU_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR2994124.1"
                     /translation="MLTGWRRGFLVGWGLLAALVGTPSGSFAADKNITLLAQRAATNP
                     TGGYGPGYSGCWGYGDSLGREFALMCGYFELDIWDVTNPANPILAKTIPTTGSDLKQV
                     RPYRHYLYAVNQSGPLQIIDVSDPYNASTVAGYLPCAAGAGSHTIHIDGHYAYLNTAG
                     SCARDLRILDLTDPLNPVEVGHYTYPGPGFVDAHDSYVYGNTCYVANLAGGVSILDVS
                     NKTQPVLQAIITYPGNFDHNCWTTTDGKYLLTTDETCQGHLRVWDVRDLSNIREVGSY
                     AAGPNHIIHNVQVNGVWAFISYYSEGVKVVDIEDPTDPIEVGSYDTYPQGSTCSYGGC
                     WDVFPYFPSGTLVASDMANGMFLLRFNGARAGRIEGRVFNVGNGQSVAGATVRLLEIG
                     KSFVTGTDGRYAFRTGDGLRTIEVSRPGFRTDTVQVTAVLSDTISADLPLTPTLQGDF
                     NGDLEVNLADVVVLINAVVFNIPPLAPQAADLTGDCLVDLVDIITLINFVVFNNVSLQ
                     SCPP"
     gene            4555..5166
                     /locus_tag="AC16FU_06845"
     CDS             4555..5166
                     /locus_tag="AC16FU_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2994125.1"
                     /translation="MEDARAREQSDPAKGGAHHELVSRFQSGDERAFNEIVLLYQKPV
                     YLVAFRMVRNHQDAQDLAQEVFVRTYSGLKKFKYEASLFTWLYRVTVNLAINFARRER
                     FKQFVSIFDVGELLTSKDDPRIDFQRDLLSRQIDGAVLKLPPRQRAVFVLRHYHDLPH
                     AEIAKIIGRTEGAVKANYFQALAKLKKFLRPESLRPVENVSAV"
     gene            5163..5759
                     /locus_tag="AC16FU_06850"
     CDS             5163..5759
                     /locus_tag="AC16FU_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2994126.1"
                     /translation="MKCGDVKNYLTDYARGALAVGLREQVSLHLEGCPACHALLAEEE
                     RLAQLLAAEQPAEPSDTFWANFLSRVRERIAVRKKKRKSYLVRRWVYVTGSVAAILVL
                     SLFAHQRWFGQNEPTGWLSQLPPGVTVEDVVGYVAARGGDGQLASAAIGADSVYIAGL
                     VSAAQESLAQEGRLELVFHQIPEGEALRVLAEVERLKL"
     gene            5777..6289
                     /locus_tag="AC16FU_06855"
     CDS             5777..6289
                     /locus_tag="AC16FU_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994127.1"
                     /translation="MKTAIARRAEGITIGALVLALVVGGSATAQMRRRPPRPPEERQK
                     WQERLATLRELKLIEVLELSDEQSNQFLPAFKAYQKKRGLVAGKRDVVLQELGLLLEE
                     EGTSDQKLQEKIDQLGELRKEQNQLDNEFFAKARTILKPRQMARMAVFEVRFERAILE
                     QLRQRSEEQP"
     gene            6315..6830
                     /locus_tag="AC16FU_06860"
     CDS             6315..6830
                     /locus_tag="AC16FU_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR2994128.1"
                     /translation="MTRKIFTGAATLVALGLLAGGAFGQPKKKERAGDQKKMEGMKLS
                     DEQKAKLQEMHFTFAKEQTMLRAKERVAALELRQMLSEDQPSREALMHKWGEVREYKN
                     QLAQRRFQELLSVRDVLTKDQREAMRHKMGEKFMQRRMHGRPGFMGPGMHRPGQMPQM
                     PMAPPEPPEPE"
     gene            7027..9210
                     /gene="rnr"
                     /locus_tag="AC16FU_06865"
     CDS             7027..9210
                     /gene="rnr"
                     /locus_tag="AC16FU_06865"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR2994129.1"
                     /translation="MSEIKTLDKNRIIEYLKKSGYQPAKTRRLAREMAIPEDLYGQFR
                     RLVRELLRAGEIVELKHNRLALPDKEKFVVGRIYGHRNGYAFLGPEKGGDDIFINPQN
                     LKTALHGDKVLATLVASRTGRSVEGRVVKILERGKKTVVGTFHHSRLADFVVPDDSRF
                     PKEIYIPPGATDGARDCQKVVVTLAPWEDEYRKPEGKISEILGYPGDPGLEILTIIRE
                     FDLPHEFPQEVEHEAAKIPEKIPAAEIKRRLDWRKKITFTIDPVDAKDHDDACSIEKL
                     ANGHWLLGVHIADVSYYVTEGSNADREALLRGTSVYLADRVIPMLPERLSNNLCSLVP
                     KKDRLTFSCLLEIDRHGKVHKSEVAESIIESRAKLAYEQVQAFFDTGKASPEIKGLEA
                     SLTEMRALARILLENRKKRGSLDFDLPEPHVLLDKEGRVIDVYQKIRLESHRLIEEFM
                     LLANQTVAELFTAKNFPILYRVHDRPAPEKMESFAAMMADFGYALHFKDEPTPFRLAR
                     FLEQVRGRPEEELVNELLLRSLKKAVYQPENIGHFGLAFDHYLHFTSPIRRYPDLLVH
                     RLGKELLRGRPTVSRFRWLEANLSKIGQITSERERVAEEAERESVKVKQLEFMQHKLG
                     EVYPGIVSGVTSFGVFVRIVALGVEGLVRFSALGDDYYIFEEEKHRLVGRRSKKVIRL
                     ADPMKVQVLRVDLLQKRLDLVPFGEEERPTAPTEKRPPQRGRRHR"
     gene            9215..10750
                     /locus_tag="AC16FU_06870"
     CDS             9215..10750
                     /locus_tag="AC16FU_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2994130.1"
                     /translation="MSPGERKILLISGDEILRRSLSDAFAKAGLTLSAAAQAQEGLKL
                     LREESFPLILLDSRPIGLEPEKLVRKIISKNPLADLVALTPAGMPDLRARLSDLGIND
                     FFDLATPPDEIVFHSRELLLQRQLLEQVGFVGKSPEIKRIAETLPQVAASDLVALITG
                     ESGTGKELLAQAIHKSSARKDKPFLAINCGAIAEGVLESELFGHEKGAFTGAIAQRPG
                     MFEVAHGGTLLLDEIGEMKPATQVKLLRVLEEKSFLRVGGKAKITVDVRVIAATNRDL
                     AAEVASGNFRRDLYYRLSVIKLDLPPLRARRGDIPLLFFYFINQLSASGHTARMGGRP
                     LEVTDEALRLLSDYHWPGTVRELKNFVERAGVLSPDGRITGREVQEYIESQSALGGLN
                     RMLPVATGKSSETVERELLYQALVGLGRQVNELRQLLISALGGGSSPLAVRTSPPQAD
                     WGNLNPSQEIIQPVTLDDVEREAIERALTEVHGNRKKAAQLLGIGERTLYRKIDKYGL
                     RKR"
     gene            10784..11542
                     /locus_tag="AC16FU_06875"
     CDS             10784..11542
                     /locus_tag="AC16FU_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994131.1"
                     /translation="MGLKEILIGVVSVIIGALLSGIGYLVKTRYEHKKLLNEALFNLL
                     EIWYVIRISTFFDVSDYMETYLRRVKDRFPEANFTKEDEEQYRQNFPKVVITSLQNFT
                     GPAKEKLHERYLGSIRKLSGIAPILAFELSGNQNLDLYLEHFDKLLNEFSPLPSDAKS
                     ESESASEMEYFRTHVKKLILSDTVHDLENDLRRLARKCGTKVWYQTVRKIHRIRKREK
                     DLPIKFADEAIDKVISPLIKKTEEIIPSSFFQND"
     gene            11594..11803
                     /locus_tag="AC16FU_06880"
     CDS             11594..11803
                     /locus_tag="AC16FU_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994132.1"
                     /translation="MRFASLIAAVLLGLISVGHLLRLIFGVEMVIEGVTLPHWASIPG
                     FIIPGAIALLLVRQERRAKRGPAAG"
     gene            12129..>13739
                     /locus_tag="AC16FU_06885"
     CDS             12129..>13739
                     /locus_tag="AC16FU_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994133.1"
                     /translation="MASACGRRFWFALLLLLLPHPASAQLAPFSLGYFFSEDELTEAF
                     RSGDLSFDDYLNLLQFIQERDQLAPSDTLLTDQIYGDRPSSVVPPANFSELKRDQLYF
                     PIEPAPERQLFGSFRSVYTDQVTLSGRQETYTNGRFQYGDEWKGNLSFQKFSSQQFVF
                     RRRSLTRTFGQGGVTVGNFEQKFGNGLLFGHHWRILDKTLLPDDDFFTLGYPTYTRYN
                     GLLFYWQPKNQARTFSLFVSTDRNANASEQLIGSTLERKFRHWTYGVGHGINRTKNAP
                     TGRSIAFSASSIYASYGREKISIQAEAAQVYQHGNGAAIEAAFEPDKDKWVFVSAWNY
                     GKNFLWPHSGGRASSDYLNVTFAENDFEYRSPQVGENGFLLKSLVQLTPRFSSEFKYT
                     NWNQFDFSGRRYRLYGALNYNDRAHFVTGYLRSDSKKASPDSLLRQRLGAEWSYQPNT
                     RLATYLNGELRGDNDALSAASEQVRIAYRLTRPLTLTGWFKYFRQWRGRNFDYWQGYI
                     AEMLELNHSLRVGAKFLADFNRTRTHERT"
BASE COUNT         3058 a         3913 c         3864 g         2904 t
ORIGIN      
        1 gcaagccaaa aaggcccggc cgacccgcct gccccagccg cccccccggc cgcggacacc
       61 ggtcaactat taagtttctt ctgcgatttc gcgcggaaag gggggagtcc gcctaaggcg
      121 gatcgaacca gcaataggcg ttcactaaga gttttgtccc gccagccgaa gggtcgtcgt
      181 tcgtgtgacg gctctttcga ctggcggggc tttttgtttt cccggcgtcc gtttggtcca
      241 gaactttccg cttgaatttc cgtattaaaa gccagacgct atattggcag acgattctta
      301 gggactggcg ggttgccatg acgctctcgg atctcgaaca cctcaaaaat ctcttgaatg
      361 aacgcgagca gaacttgacc gcgtggctcg acgcctccgg ttcgccgaac ggggaggacg
      421 tccagaaggc gcaggcgctt ttgacggata ttcgggaggc cttggggcgc gtcgaaaacc
      481 agacgttcgg cgcctgtcag ggaagcgctc cggcgggaat ttgtaagggg gaagtggaac
      541 tctaccggct ggaggtgcag ccggtgaccg aggtctgcct gggttgcatc accaaagagg
      601 aacaggcacg gttagaagag gaactgtttt tggccagcaa aatccaccgg gcactcctgc
      661 cgcagtcgat cgaaagaatt gagggctatg acgtgggggt gaaggccttg gccgcccgga
      721 gcgtgggcgg cgactactac gattttctgc cttcggccaa cggcggccgc ttgcgggtag
      781 tcattgccga caccatgggc aaaggcatcc cggccgggct tttaatgtcg catgtccagg
      841 gcgcgcttaa aattttatcc gaagacatcg agtcgccccg cctgctcatc agccgcctga
      901 accagtggct ctgccgcaac gtccccgtca ccaagtttgt ctctttaagc tgcctcgcgt
      961 tggattcccg cgccggccgg gattctcaga tcacctacac caatgccggc cactgtccgc
     1021 cgattttagt gcgaaatgat ggtaaggttg agcgtctgga gccgaccggc ggggttttag
     1081 gcgtacacga gggctttacg tacgaagagc acacattgtc gctttctccc ggtgatcttt
     1141 tggtgctcta cacggacggc gtcaccgagg ccgagaacta ccacgggggg atgttcggcg
     1201 aagagcggct tatcgaattc gcccagaccc gccgcaccga tccgctcgat aagttcctcg
     1261 acgaccttct gggcgaagtc cagttcttct ccgagaaacc ggagatggat gacgatttta
     1321 cggtgattgc cctgcggaag accgcttcat agtaaaatat gacccctccc taccctcccc
     1381 tccaagggga gggaaaagca cctgtcattt taccagagta agtccgggca attttctccc
     1441 tctcctttga ggagagggcc ggggagaggt cagttcgtag cctagccctt aatgggcgtt
     1501 gctttgaccc ctccctaccc tcccctcaaa ggggagggat ttttttattc cctttattat
     1561 tggttgaaga atttttctcc ctctcctttg aggagagggt tggggagagg tcgtagccga
     1621 ggattttatc ctcggcgccc gacagggcgt gggtcttgtc ttttgcccgt tcatcctgca
     1681 tttttcttct atgtctccgc aggaattaac cgcctacttc gacgaaattt acgcgctcaa
     1741 cctggcctgc ctcgatttag tgccggaaga gaaacttgat ttccagccgg tgccggaagt
     1801 gaaatcgctg cgcctcttgc tccaccatat gtacgtgaac cagaagttct acctccttac
     1861 cgcccggaaa gggacaatgg acgttgacga ctacaaagcg atgatgaagc aggtgccgga
     1921 taccaaagaa tcgctgcggc gggaaatggg ggaaacgttc gctgaattta agcaggcgct
     1981 gggcgacgaa gccctgctca aaaaagaggt ccagacctta tccggctcgc gccgtgtcta
     2041 ccacctgttc attggtgaat tagagcacca actgcaccac agagggcagg tctacaccta
     2101 cctgcggctc ttgggcctca aaccgccaga tagcgggttg tatatgggat tagcgagcta
     2161 agtaataatt ggttcatgcg gatcgacccg ctaatttctc actatgaaat cccacactga
     2221 atacctctgg ttcaacacga agaagcatcg ggagttcatc aacatcaccg gtgaggtgga
     2281 gaaagcgctg ggtaaaagcg gcattaaaga gggcttcgca ttagtctccg cgatgcacat
     2341 caccgcctcg gtctacgtca acgacgcaga atcgggctta atcgccgaca tcgaagaatg
     2401 gctggagaag ttagccccgt ccggggtcaa ctaccggcac caccgtaccg gcgaaaccaa
     2461 cggcgacgcg cacctgaaaa atctcctgat gcaccacgag gtcatcgtcc cggtcaccgc
     2521 cggcaaactc gattttggcc cctggcagca gatttactac gccgagttcg acggccaacg
     2581 caagaagcgg gtagtcataa aaattattgg tgattagcga ttctttgcgg ccacgcccac
     2641 aaggggctaa gctacgaccc ctccctgcat ccctcccctc aaaggggagg gacttttttc
     2701 tccccctaat tcctgactcc caaatcctga ttccgaatgg accgggcaca aaccggttga
     2761 cttttgtgtt cttttctata tactctgtcg ggagaggggc aatcccgccg gtgcgggagg
     2821 gcctttcttt ttgcgggtca gtggtgcggt taagatgtga aaggttttaa tccggtcgtg
     2881 agaggcaaat gctgacggga tggcgcaggg gatttttggt cggatggggg cttttggcgg
     2941 ctctcgttgg gacgccgtcc ggaagtttcg ctgccgataa gaacatcacc cttttggccc
     3001 agcgcgcggc cacgaatccc actggcggct acggtcccgg ctactccggc tgctggggct
     3061 atggggatag tttaggccgt gagttcgctt taatgtgcgg ctactttgaa ctcgacatct
     3121 gggatgtcac caatccagcc aatccgatcc tggccaaaac tattccgacc accggctcag
     3181 atctcaaaca ggttcgcccg tatcgccact acctctatgc cgtgaaccag agcgggccgc
     3241 tgcaaataat tgacgtgtct gatccataca atgccagcac cgtcgccggc tacctcccct
     3301 gcgccgctgg agccggctcc cacaccattc acatcgacgg ccactacgcc tacctcaaca
     3361 cggccgggtc ctgcgcccgc gacctgcgca tccttgatct caccgaccca ctcaatccag
     3421 tcgaagtggg gcactacacc tatcccggcc ccggctttgt ggatgcgcac gactcctacg
     3481 tttacggcaa tacctgctac gtggcgaacc tggcgggcgg ggtgagtatc ctggacgtaa
     3541 gcaataagac gcagccggtg ctgcaggcga taattactta tccggggaac tttgaccaca
     3601 actgctggac gacgacggat gggaagtacc ttttgaccac cgatgagacc tgccagggcc
     3661 acctgcgggt ctgggacgtg cgcgatctca gcaacatccg ggaggtgggg agctacgcgg
     3721 ccggaccgaa ccacatcatt cacaacgtcc aggtaaacgg ggtgtgggcc ttcatttcgt
     3781 actattccga aggggtcaag gtggtggata tcgaagaccc cactgacccg atcgaagttg
     3841 gttcgtatga cacgtatccc cagggttcaa cctgcagtta tggcggctgc tgggacgtgt
     3901 tcccctattt tccatcgggc acgctggtcg cgagcgacat ggccaacggg atgttcctct
     3961 tgcgcttcaa cggggcgcgc gcaggtcgaa ttgaaggcag ggtgttcaac gtagggaatg
     4021 gccaatcggt ggccggcgct accgtccgcc ttctggaaat cggcaaaagt ttcgtgaccg
     4081 ggaccgatgg gcgctacgcc ttccgcaccg gtgacggcct gcgcacaatc gaagtgagtc
     4141 gaccgggttt tcgtaccgac actgttcagg tcacggcggt tttgagtgac accattagtg
     4201 ccgacttgcc cctcacccca actctgcagg gggacttcaa tggtgacctg gaggtaaatc
     4261 ttgccgatgt ggtagttctg atcaatgccg tggttttcaa tatccccccg cttgcacctc
     4321 aggcggctga cctgaccggc gactgcttgg tcgatctggt ggacatcatc acgcttatca
     4381 actttgtcgt attcaataac gtttccctgc agtcctgccc tccctgaggg atgacaagga
     4441 gagagacccc cggttttgac cgggggtttt cttttggcgg aggtttggca agccccgctc
     4501 tgagggttaa actattccca gggtgaccgg tctaatacga tagcaggcgg tgagatggag
     4561 gatgccaggg cgcgggagca atccgacccc gccaaaggcg gggcgcacca cgaacttgtc
     4621 agccggtttc agtccggcga tgagcgggct ttcaacgaaa ttgtcctcct ctaccagaag
     4681 ccggtgtatc tggtggcctt ccggatggtg cgcaaccacc aggacgcgca ggacttggcg
     4741 caggaggtgt tcgtgcggac ttactccggg ttgaaaaagt tcaagtacga agcctcgctt
     4801 ttcacctggc tctaccgggt gacggtgaat ctcgccatca acttcgcccg gcgcgagcgg
     4861 ttcaaacagt tcgtgtcgat tttcgacgtc ggtgagctgc tgacgagcaa agacgatcct
     4921 cggattgact ttcagcgcga ccttctttcg cgccagattg atggggcggt gctcaaactg
     4981 ccgccgcggc agcgagcggt cttcgtcctg aggcactatc acgatctgcc ccatgcggag
     5041 attgccaaaa tcatcggccg gaccgaaggc gcggtcaaag cgaactactt ccaggcgctg
     5101 gcgaaattga aaaagttttt gaggccggaa tcactccgac cggtggaaaa cgtgagtgcg
     5161 gtatgaaatg tggagacgtt aaaaactatc tgaccgatta cgctcgcggc gccctcgccg
     5221 tcggtttgcg cgagcaggtt tctcttcacc tggaaggttg cccggcctgt cacgcgctcc
     5281 tggcggaaga agagcgcttg gcgcagctct tagccgccga acagcccgcc gagccctccg
     5341 acaccttctg ggccaacttc ctgtcccggg tgcgggaacg gatcgccgtc cggaaaaaga
     5401 aaagaaaaag ttatctcgtt cgtcgttggg tctatgtcac cggcagcgtc gccgctattc
     5461 tggtgctcag cctttttgcc catcagcggt ggttcgggca gaatgaacca accggctggc
     5521 taagccagct gccgccaggg gttaccgtcg aggacgtcgt cggctacgtg gccgcgcggg
     5581 gaggggatgg acaactggca agtgccgcca tcggcgccga cagcgtctac atcgccgggc
     5641 tggtgtctgc cgctcaggag tcgctggcgc aggaggggcg tctggaatta gtcttccatc
     5701 aaattcccga gggggaagcc ctgcgggtgc tggcggaagt ggagcgtttg aaactctgaa
     5761 aaagggcagg tttacgatga aaactgcgat agcgagacga gcagaaggca tcaccatcgg
     5821 cgctttggtg ctggcactgg tggtgggtgg cagcgccacc gcccagatgc gccgccggcc
     5881 accgcgtccc ccggaggagc gtcagaagtg gcaggaacgg ctggcgactt tgcgcgaatt
     5941 aaaactgatc gaagtgctgg aactctccga cgagcagtcc aatcaattcc tcccggcctt
     6001 caaggcctac cagaagaagc ggggtttggt cgccggcaag cgcgacgtgg tgttgcagga
     6061 actgggtctg ctcttggaag aagagggtac ctccgaccag aaactgcagg agaaaatcga
     6121 ccaattaggc gagttgcgga aagagcagaa tcagctcgac aacgagtttt tcgccaaagc
     6181 gcgcaccatc ctcaaaccgc gccagatggc gcggatggcg gtcttcgagg tgcgctttga
     6241 gcgggccatt ttagaacagc tgcgacagcg atccgaagag caaccctaaa gataatgaaa
     6301 ggatggtgag aaagatgacc aggaagatct tcaccggagc agccacgctc gtggccctgg
     6361 gacttctggc aggcggcgcc ttcggtcagc cgaagaaaaa agaacgcgcg ggcgaccaga
     6421 agaaaatgga ggggatgaaa ctctccgacg aacagaaggc gaagctgcaa gagatgcact
     6481 tcaccttcgc caaggagcag acgatgctcc gggccaaaga gcgggtggcc gcattggaac
     6541 tgcgccagat gctctccgaa gaccagccca gtcgtgaagc gctgatgcac aaatggggtg
     6601 aagtccggga gtacaaaaac cagctcgccc agcgccgctt ccaggaactc ttgtcagtgc
     6661 gagacgtgct gaccaaagac cagcgcgaag cgatgcgcca caagatgggg gaaaaattca
     6721 tgcagcgccg gatgcatggc cgacccggat ttatgggccc cggaatgcac cgcccgggcc
     6781 aaatgccaca aatgcccatg gctcctccgg agccacccga accggagtga ccaccctcct
     6841 ctgggaaaaa gccccaccga ttggcggggc ttttcatttg attgaacagg cacctcggtg
     6901 gtagtccggc tcgtcctcgt acgagtcgtg ccgaccagag ccggattgtc gggctcgggg
     6961 acgagcccga caatcagtta aaaacctctt tgactaaacg ttctttttga cttattttct
     7021 gccaaaatgt cagaaattaa aactttagac aagaaccgga tcatagaata ccttaaaaag
     7081 tccggttacc agccagccaa aactcgcaga ttagcccggg aaatggctat tccggaagat
     7141 ctgtacggcc agttccggcg gttggtgcgt gaattactcc gcgcggggga gattgtcgag
     7201 ttgaagcaca accggctggc tttgccggac aaagagaagt tcgtcgtcgg ccggatttac
     7261 gggcaccgga acggctacgc ctttctgggg ccggagaagg gcggggatga catcttcatc
     7321 aatccccaga atctcaagac cgccctgcac ggcgacaaag tcttggcgac attggttgct
     7381 tcccgaacag gcagaagtgt ggaaggacgg gtggtcaaaa tcctcgagcg cgggaagaag
     7441 accgtggtag gcaccttcca ccacagccgc ctcgccgact tcgtcgtacc cgacgattcc
     7501 cgcttcccga aagaaattta catcccgccc ggagcgaccg acggcgcccg cgactgccag
     7561 aaagtggtcg tcacgctcgc cccctgggaa gatgaatacc gcaaaccgga aggaaagatt
     7621 tcagaaatcc tgggctaccc cggcgatccc ggtctcgaaa tcctcaccat catccgggag
     7681 ttcgatctcc cgcacgagtt cccgcaagaa gtggagcacg aggcggcgaa aatcccggag
     7741 aaaattccgg ccgccgaaat caagcgccgg ctcgattggc gcaaaaaaat caccttcacc
     7801 atcgatccgg tcgacgccaa agaccacgac gacgcctgct ccatcgaaaa actggccaac
     7861 ggccactggc tcttgggcgt ccacatcgcc gacgtttcgt actacgtcac cgagggctcg
     7921 aacgccgacc gcgaggcgct cctgcgcgga accagcgtct atttggccga ccgggtgatt
     7981 ccgatgctgc cggagcggct ctcgaacaac ctttgcagtc tggtgccgaa gaaagatcgc
     8041 ctgaccttct cctgtcttct tgaaatcgac cggcacggca aagtccacaa atcggaggtg
     8101 gccgaatcga tcatcgagag ccgggcgaag ctggcctacg agcaggtgca ggcgtttttc
     8161 gacaccggca aggcctcacc cgaaatcaaa ggactcgaag ccagtctcac cgagatgcgc
     8221 gctttggccc gcattcttct cgaaaaccgc aagaagcgcg gcagtctcga tttcgacctc
     8281 cccgagccgc acgtcctcct ggacaaagag ggccgggtga tcgacgtcta ccagaaaatc
     8341 cggctggaaa gccaccggct gattgaagag ttcatgcttt tggccaatca aacggtggcg
     8401 gaacttttca ccgcaaaaaa tttcccgatt ctctaccgcg tgcacgaccg acccgctccc
     8461 gaaaaaatgg aatcgtttgc cgcgatgatg gccgatttcg gttacgcgct ccatttcaaa
     8521 gatgaaccga cgccgttccg tctcgcccgc tttttggaac aggtgcgcgg ccggcccgag
     8581 gaggagttgg tcaacgaact cttgctccgc tccctcaaaa aagcggtcta ccagccggag
     8641 aatatcggcc acttcggcct ggcctttgac cactacctgc acttcacctc ccccatccgg
     8701 cgctatcccg atctcttggt ccaccgcctc ggcaaggaac ttctccgtgg ccgtccgaca
     8761 gtgtcccgct tccgctggct ggaggccaac ctgagcaaaa tcggccagat cacttccgaa
     8821 agggagcggg ttgccgagga agccgagcgg gaatcggtca aagtcaagca attggaattc
     8881 atgcagcaca aattgggcga ggtttatccc ggcatcgttt cgggcgtcac cagcttcggg
     8941 gttttcgtcc gcatcgtggc cctgggcgtt gaagggctcg tccgtttctc cgctctggga
     9001 gatgactatt acatcttcga agaagaaaag caccggctgg tggggcggcg ctccaagaag
     9061 gtcatccgtc tcgctgatcc aatgaaggtg caggtgctcc gcgttgacct cctgcaaaag
     9121 cgtctcgatt tagttccttt cggcgaggag gagcggccga ctgctcccac cgagaaacgt
     9181 cctccgcagc gcgggcggcg gcacaggtga ggttatgtct ccgggcgaaa gaaaaattct
     9241 cctcatctcc ggcgacgaaa ttctccgccg ttcgctcagc gacgcttttg ccaaagccgg
     9301 tttaaccctc tctgccgcgg cgcaggcgca ggagggattg aaactcctcc gggaagaatc
     9361 gtttccgctc atactgctgg acagccggcc catcggcttg gagccggaaa aattagtccg
     9421 taaaattatc agcaaaaatc cgttagccga tctggtcgct ctcacccccg ccggaatgcc
     9481 cgacctgcgc gcgcggcttt ccgatctcgg cattaacgat ttctttgacc ttgccacgcc
     9541 gcctgatgaa atcgtcttcc acagccgtga actgctgttg cagcggcaac ttttggaaca
     9601 ggtcggcttt gtcggcaagt cgccggagat caaacgcatt gccgaaacgc tgcctcaggt
     9661 ggccgcgagc gacctggtcg ccttgatcac cggcgaatcg ggcacaggca aggaactcct
     9721 tgcgcaggcg attcacaaaa gttcggcgcg gaaagataaa ccgttccttg ccatcaactg
     9781 tggcgctatc gccgagggag tgttagagag tgaactcttc ggccatgaga aaggcgcctt
     9841 caccggcgcc atcgcccagc ggcccggaat gttcgaagtc gcccacggcg gcacgctcct
     9901 cttggacgaa atcggcgaaa tgaagcccgc cacgcaggta aaactcctcc gggtgttgga
     9961 agagaaatcg ttcctgcggg tgggggggaa ggcgaaaatc accgttgacg tgcgggtgat
    10021 tgctgccacc aaccgcgacc tggccgccga agtcgcctcc ggcaatttcc gccgcgacct
    10081 ctactaccgg ctctcggtca tcaaactcga cctgccgccc ttgcgcgcgc gccggggcga
    10141 cattccgctg ctcttcttct acttcatcaa tcagctctcc gcctcaggcc atacggcccg
    10201 tatgggcggg cgcccgctgg aggtcaccga cgaagcgttg cgcctccttt ccgactacca
    10261 ctggccgggg accgtgcggg aattgaagaa tttcgtggag cgggccgggg tgctctctcc
    10321 cgacggccgc atcaccgggc gggaggtgca ggagtatatc gaaagccagt ccgccttagg
    10381 tggattgaac cgcatgctgc ccgtggcgac gggcaaaagt tccgaaaccg tcgagcggga
    10441 actcctctac caggcgttag tggggctggg ccggcaggtg aatgaactta gacagttgct
    10501 tatttccgcc ctgggcggtg gctcaagccc gctcgccgta aggacgagtc cgcctcaggc
    10561 ggattgggga aacctcaatc cctctcagga aatcattcag cccgtgacct tggacgacgt
    10621 ggagcgcgag gccattgagc gcgccctcac cgaagtgcac ggcaaccgca agaaagccgc
    10681 gcaacttttg ggcattggcg aaaggacgct gtataggaag atagataagt atgggttgag
    10741 gaaacgctga ggaaatacaa aagagcacga ggtactattt taaatgggct taaaagaaat
    10801 tttaataggt gtagtaagtg taataatcgg ggcgttatta agcgggattg gctatctagt
    10861 taagactagg tacgagcaca aaaaacttct gaatgaagca ctgttcaatc ttctagagat
    10921 ttggtatgtt attcgaattt ccactttttt tgatgtcagt gattatatgg aaacttatct
    10981 tcgtagagtt aaggaccgct ttcctgaagc taactttacc aaggaagacg aagaacaata
    11041 tcgacaaaat tttcccaaag tagtaattac ctcattgcag aattttactg gacctgcaaa
    11101 agagaaatta catgagcgat atcttggatc aatacgaaag cttagcggta ttgctcccat
    11161 ccttgctttt gagttaagcg gaaatcaaaa tttggattta tatttggaac atttcgacaa
    11221 acttctaaat gaatttagcc cattgccatc tgatgccaaa agtgaaagtg agagtgcttc
    11281 tgaaatggag tactttcgaa cacacgttaa aaaacttatc cttagtgata cagtgcacga
    11341 tttagaaaat gacttacggc gactggcacg gaaatgtgga accaaagtgt ggtaccaaac
    11401 tgtgcggaaa atacaccgga ttagaaagcg cgagaaggat ttaccaataa agtttgcgga
    11461 tgaagcaata gataaagtca tatccccact gattaagaaa actgaagaga ttattccgtc
    11521 atcttttttt caaaatgact aggggtaaac cacatatcat agaacgtagt aaagcccact
    11581 tcaggaggtt actatgcgct tcgcttctct gatcgccgca gttcttctgg gcctcatctc
    11641 cgtcggccat ctccttcgct tgatattcgg cgtggaaatg gtaatcgaag gggtaacgct
    11701 tccacattgg gcaagcatac ccggctttat tataccgggc gctattgcgc tcctcttggt
    11761 gcggcaggag cggcgggcaa aacggggccc ggccgccggc tgagcggctt agaagcaggt
    11821 tatgggtgag agagatgacc cctccctgta tccctcccct acaggggagg ggaaaatcca
    11881 tctttatggg aggttagtct tatctttccc tctcctcgta gtggttcgac ccgtacgggc
    11941 tcaccaccct gagcatgtcg aaggggagag gggaggtcga agacgcgcct caggcggggt
    12001 gaggtcaaag agtgctaaat ctgacccctc cctgtatccc tcctctcata ggggagggaa
    12061 aattcgtctc ttttcttttg ccgcccctgc gcttgcgcct gaaatgagta agacgtaaaa
    12121 ttggcctaat ggcctctgcc tgcggcaggc ggttttggtt tgcgcttttg cttcttctcc
    12181 tgccacaccc cgcctccgcc cagctcgcac ccttttccct cggttacttc ttctccgaag
    12241 acgaactgac cgaggccttc cgctccggcg acctctcctt tgacgactac ctgaatcttt
    12301 tgcagttcat tcaggaaaga gaccagttag cgccgtcgga cactctccta actgaccaga
    12361 tttacgggga tcgtccttct tccgtagttc ccccggcaaa tttctctgaa ctgaagcgag
    12421 accaacttta ctttccaatc gagcctgcac cagaacgcca actcttcggc tcattccgct
    12481 cggtctacac cgatcaggtg accctctccg gtcggcagga aacctacacc aacggcaggt
    12541 ttcagtacgg tgatgaatgg aaagggaatc tgtcgtttca aaaattctcg agccagcaat
    12601 tcgtctttcg ccgccggtct ttgacgcgca cgtttgggca aggcggcgtc accgtcggca
    12661 acttcgagca gaaattcggc aacggtctcc tcttcggcca ccactggcgc attttggaca
    12721 aaaccctcct gcccgatgac gatttcttta cactgggcta ccccacctac acccgctaca
    12781 acggcctttt gttctattgg cagcccaaaa accaagcacg gacgttcagt ctttttgtct
    12841 ccaccgatcg caatgctaat gcctccgaac aactgattgg cagcacgctt gaacgtaagt
    12901 ttcgacactg gacttatggt gttggacacg ggatcaatcg aacgaagaat gcaccaaccg
    12961 ggcgttctat tgctttttca gcgtcgagca tatatgcgtc ctacgggcgc gagaaaatct
    13021 caattcaggc agaggcggcg caggtttacc agcacggaaa cggagcggct atcgaggccg
    13081 ccttcgaacc ggataaggac aaatgggttt tcgtatccgc ctggaactac ggaaagaatt
    13141 tcctctggcc ccactccggt ggccgcgcca gttccgatta cctcaatgtc accttcgccg
    13201 agaacgactt cgaataccgc tcgcctcagg tcggggagaa cggttttttg ctcaagtcgc
    13261 tcgtccaact gaccccgcgt ttcagcagcg agttcaaata caccaactgg aatcagttcg
    13321 atttctcagg ccgccgctat cgcctctacg gcgcattgaa ttacaatgat cgagcacatt
    13381 tcgtcaccgg ctacctgcgg agtgattcga agaaagcctc acctgattcg ctcctgcgcc
    13441 aacgcctcgg cgcggagtgg agctatcaac caaatacgcg cctggccact taccttaatg
    13501 gcgaactgcg gggagataac gatgcgctgt ccgccgcaag cgagcaggta cggattgctt
    13561 accgcttaac ccggccgttg acccttacgg gctggttcaa gtattttcgc cagtggcggg
    13621 ggagaaattt cgattactgg cagggctata tcgccgaaat gttggaactc aatcactctc
    13681 tgcgcgtcgg cgccaaattc ttagccgact tcaaccggac gcgaacacat gagaggacg
//
LOCUS       DBNGZA010000314         1518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2073720, whole genome shotgun sequence.
ACCESSION   DBNGZA010000314 DBNGZA010000000
VERSION     DBNGZA010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2073720"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1452)
                     /locus_tag="AC16FU_06890"
                     /pseudo
     CDS             complement(202..1452)
                     /locus_tag="AC16FU_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193729.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
BASE COUNT          267 a          475 c          427 g          349 t
ORIGIN      
        1 ggccaggcgc agcaccaggg ccaagacgaa caccagtgct aatccggcgc gccaggagag
       61 cacccgctcg gaaaacgttc gcgtttgtga aaaaggaact cctaagtcca ctttttattt
      121 ctttgagcgt ctcgacttcc ctgtttttct cggagtctgc ggtgccggtc gcgctttcca
      181 gatttttccc cgcccggatt tcacaaactg ccgcgggtcg gtaatttctc cggtgatggc
      241 tgaggcgacc accgtcgccg gtgacgcgag gtaaatgaac gcctccgggt tccccatccg
      301 gcctttgaag ttgcggttca tggtggtgag tgccacttcc ccatcagcgg ggactcctgc
      361 gtgaacgccg acgcacggtc cgcacccggg cggcgtgatc accgccccga attccaagag
      421 cgcctggtag taccctttcg ccagcgcctc ctccatcacc cagcgggaag cgggagtcac
      481 gatgaatttc accctcggat gttttttcct ccccttgcag atggaagcgg cgatctgcca
      541 gtcttcaatc cgtgagttcg tgcaggtgcc caaaaagacc tgatcgactt ttatgccttt
      601 cgcctcgctg atcggatgcg tgttatcgac cgtgtgggga aacgacacgg ttggttccaa
      661 ttttgacgcg tcaatctcaa tcgtccgctc gtagaccgcg tctttatccg gcctgatttc
      721 ccgccatttc tcgggccggc ccatcttctt caggtactgg tgggtgatcc ggtcggaggc
      781 gatcattccc accttcgcgc ccgcttccac cgccatgttt gagagcgtca ggcggtcgtg
      841 catcgccatc gcctcgatgg tcggcccgca gaactccagc gccttgtagg tggcgccgtc
      901 ggcgccgatc aagccgatca ggtgtaaaat caaatctttt gggtagactc ccggagaaaa
      961 actgccccgg atggagattt tgaatgactc cggcacccgc agccaggtgc ggcccagggc
     1021 gatggcaatg gccacgtcgg ttgaccccat gccggtggca aaagcgcaca gggcgccgcc
     1081 ggtgcaggtg tggctgtcgg ccccgaccaa aatatccccg gggctgacgt gtcattccgc
     1141 caccacctga tggctgattc cttcgccgat ttcggagacc tgcaccccgc tttgcgccgc
     1201 gaactggcgg atgaagttat ggtcgttggc caattccttc cgcgacgccg gtgccgagtg
     1261 atcgaggaaa aaaatcaccc ggttgggatt ggccgccttt ttgactccca atttctggat
     1321 ctgccggatg gtgagcggcc cggtgccgtc ctgtgccagc gccagatcga ctggcgtgat
     1381 gaccagttcg cccgcccgta cgggatggcc gaccttccct gctatgacct tttcggctaa
     1441 cgtcgcaccc atgttctcct cttttctatg gaatgaatgg aaactatact tttccggccc
     1501 tacgtttgaa cttgctct
//
LOCUS       DBNGZA010000315         1339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2073734, whole genome shotgun sequence.
ACCESSION   DBNGZA010000315 DBNGZA010000000
VERSION     DBNGZA010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2073734"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1339)
                     /gene="purL"
                     /locus_tag="AC16FU_06895"
     CDS             complement(<1..>1339)
                     /gene="purL"
                     /locus_tag="AC16FU_06895"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR2994134.1"
                     /translation="YGNSFGVPTVGGEIYFEDCYTGNPLVNAMAVGLIKKGRMVRAVA
                     KGVGNPVVIVGAATGRDGIHGATFASEEISEASEARRPSVQVGDPFLEKLLLEATLEI
                     IKEDLVVGIQDMGAAGLTCSTSEMAARGGCGLEVELAHVPLRETGMTPYEIMLSESQE
                     RMLVVVKSGSEKRVSEIFTKWGLNSAIIGKVTADGLLRVKVHGRVVAEIPANSLVLGG
                     GAPVYVREKKKPEYIDQLQQIDFSTVPAPADFNNVLLKLLASPNIASKEWVTRQYDTS
                     VRTNTVVKPGSDAAVLRLRKTNKALAVVTDCNARFCYLNPRRGAMIAVAEAARNVVCA
                     GARPLAITNCLNFGNPYDPEVFWTFAEVIAGMGAACRAFETPVTGGNVSFYNEDPERA
                     IYPTPVIGMVGLVEDVKHITTQWFKDEGDVILLLGENREELGGSEYLKTIHGI"
BASE COUNT          272 a          446 c          339 g          282 t
ORIGIN      
        1 atgatgccat gaatcgtctt cagatattcg gagccgccca attcttcccg gttttcgccc
       61 aaaaggagaa tgacgtcgcc ttcgtcctta aaccactgcg tggtgatatg tttcacatcc
      121 tcgaccaaac ccaccatccc gatcaccggc gtggggtaaa tcgcccgctc agggtcttcg
      181 ttgtagaaac tgacgttccc gcccgtcacc ggcgtctcga acgcccggca ggccgccccc
      241 atcccggcaa tcacttcggc aaacgtccaa aaaacttccg gatcatacgg gttgccgaag
      301 ttcaaacagt tggtgatggc cagaggccgc gccccggcac agacgacgtt tctggccgcc
      361 tccgccaccg caatcattgc cccgcgccgg gggttgagat agcagaaacg ggcgttgcag
      421 tcggtgacga ccgccagcgc cttgttcgtc ttccgcaggc gcagcaccgc cgcatccgac
      481 ccgggcttaa cgaccgtatt ggtccgcacc gaggtatcgt actgccgggt tacccattcc
      541 ttcgaggcga tgttgggcga tgccagaagt ttcaggagga cattattgaa atcagcaggt
      601 gcaggaacgg ttgaaaaatc aatttgttgc agttggtcga tatattcggg ttttttcttt
      661 tcccggacat agaccggcgc tccaccgcct aaaacgagcg aattcgctgg aatctcagcc
      721 acgaccctcc cgtggacttt aacccgcagc aggccgtcgg cggtcacctt cccgatgatg
      781 gccgaattga gcccccattt ggtgaaaatc tcgctgacgc gcttctcgct gccggacttg
      841 accacgacta acatccgctc ctgcgactcg gagagcatga tttcataggg ggtcatcccc
      901 gtctctcgca gcgggacgtg agccaattca acctccagtc cgcagccgcc gcgcgccgcc
      961 atttcggaag tagaacaggt cagcccagcc gcccccatat cctggatgcc gacgactaaa
     1021 tcttccttga tgatttccaa agtcgcttcc aagagcaatt tttccaggaa cggatcgccc
     1081 acctgcaccg agggccgcct ggcttccgat gcttccgaaa tttcttccga ggcaaaggtc
     1141 gctccgtgaa tcccgtcccg ccccgttgcc gccccgacta tgaccaccgg attgccaaca
     1201 cctttggcca ccgcccgcac catccgcccc tttttgatga ggcccaccgc catcgcattg
     1261 accagaggat tccccgtgta gcagtcttcg aaatagattt ccccgcccac ggtgggcacg
     1321 ccgaaactgt tcccgtagt
//
LOCUS       DBNGZA010000316         7248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_273073, whole genome shotgun sequence.
ACCESSION   DBNGZA010000316 DBNGZA010000000
VERSION     DBNGZA010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_273073"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC16FU_06900"
     CDS             <1..187
                     /locus_tag="AC16FU_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994135.1"
                     /translation="AERTFLLKAFPLPDRFDPLHAIADPIGKGPEEYEKSFLESDEHL
                     RRICSLVVERAREKNAG"
     gene            177..1094
                     /locus_tag="AC16FU_06905"
     CDS             177..1094
                     /locus_tag="AC16FU_06905"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR2994136.1"
                     /translation="MRAKQSLPWKIIFSALLPWGALIWVGPLPGGAGVGGTELVPVGD
                     STFRDSTRKGNIPADPSGADTLPTEIPPPLRMVENYSFGVGERLEYDITYGLFTAGSA
                     VMEVKELADVNGYPCYHVTSTARSNALVSKFFAVEDYVHSFVDARGVFTWRFEKKIQE
                     GKYKTHRQALYDQINHRVVTERNDTLAVAPFVQDALSALFFVRTQKLEVGKSLWVEAF
                     SDGKKYPLEVRVHKKEKIRVPAGEFRCIVVEPLLKTSGIFEHKGRLTVWLTDDRLKLP
                     VLMKSKVIVGNIAAELTAYTLGRPERFRR"
     gene            1091..2074
                     /locus_tag="AC16FU_06910"
     CDS             1091..2074
                     /locus_tag="AC16FU_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2086 family protein"
                     /protein_id="HIR2994137.1"
                     /translation="MKYEALDLAQVRTYSIRKRKHLARQTDFAGIPEPHDSIGQFLAI
                     LPQVLKAKNFVSLADTLAAAARKRPVIALLGAHVIKCGLSPVIIDLIRNGLLTHVAVT
                     GAGAIHDLEVAFFGQTSEEVAAHLQDGSFGMVRETAAIFNAAVAAGEGAGLGERLGYF
                     FLVRKPKFLRYSILAAAAEQKMPATVHVAFGTDTLHQHPGFDPASVGLLTHHDFKVLA
                     ASVGRLRPGAVVLNIGSAVVLPEVFLKALAVARNIEGARKVKGFTAANFDMIQHYRPN
                     VNVVGRPTLGGGTGYSFTGHHEIMLPLLAWAVKAKMQHTIGRTRNTGKSKR"
     gene            2118..3641
                     /gene="ftcD"
                     /locus_tag="AC16FU_06915"
     CDS             2118..3641
                     /gene="ftcD"
                     /locus_tag="AC16FU_06915"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR2994138.1"
                     /translation="MPRLVECVPNFSEGRRPEVIDALQREIAAVPGVALLDREMDKDH
                     NRAVLTFIGEPEAVKKAAIRAIAKAAELIDMEEHKGEHPRIGATDVVPFVPISEVTMD
                     ECVQLAHQLGQEVGEKLGIPVYLYEAAATRPDRMNLANIRKGEYEGLKEEIGKNPDRK
                     PDFGPERLHPRAGATVIGARMPLIAYNIYLGTNDVSIAKNIAKKVRESSGGLPAVKAL
                     GFAIKERNLVQVSMNLTNYLKTPIHEAVEAVKAEAGRLGVPVVSTELVGLAPLDSFLA
                     SAEYYLKLEHFKRDQILETKLLSLFSERRESLADFVDRVASAEPTPGGGSVAALCAAL
                     AAALTAMVCRLTLGKKKFAAVETEIKEVLQKAEQLRQVTFQLIQKDTAAFQQVMEGYK
                     LPKETEKEKESRDRAIEKATLQASEIPLEVMRRGLEVLRLSDVVAEKGNPNSRSDAGV
                     AALLARAATEGAWLNVEINLQSIRDAEFVKRVTEEGQKILSQARLPRGNVQTVLAAN"
     gene            3671..5503
                     /locus_tag="AC16FU_06920"
     CDS             3671..5503
                     /locus_tag="AC16FU_06920"
                     /inference="COORDINATES: protein motif:HMM:NF045524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6640 family putative metalloprotease"
                     /protein_id="HIR2994139.1"
                     /translation="MRCFGVTILLFFLSNVPQSQAKLSQAYEAGLIERVKSLRPAQSG
                     VVLAGAEGLPRPLCGTPIALEANYNYENLSSETQSVLAQYLARPTTGVTQITPLGFIR
                     VHYDTASGDVDRVPSADVLPLNGIPDFIDDAMTVLDSVWLREIVDLGYRKPLVDGTAG
                     GDSLLDVYFQQLGPSYLGQTFVDVGNLGPQRNQGTAYVELDNDYVFPQYPTPAARQRI
                     LRVTAAHEFFHAVQFAYDIREFEFTDPGNSSTFRPYWYEMSSTWMEDIVYDTIDDYHS
                     LVKYVLRYPWLSFRTFSYNPSDTAGVFHPYAQALWNHYLVARFADTTLIRTIWTLCGA
                     VNGFNFFPAVNQALSAKMSSFLASFREYCIWNYYTGSREVPGKYYRLEGESFPVGAFV
                     DTLPQQSVIVGDAQPPVQDLGCTYYFIVPNASQPDSFQIFFTVLSGAVAQWDIAGFGR
                     DTTGDTVFISFMPVGAASWLARYPAWKTFTDQVVLPIALQEPIDATIGNYGYHYVFGF
                     QGPPPASDQFSDPYPNPIFLPQQTTVRFKVNLARDAQVEFSVHDLSGQVIHEERPKKF
                     LAGVESFLTWDGLNDEQQMVASGVYLCAIRVGDKTKVFKLAVVR"
     gene            5500..6024
                     /locus_tag="AC16FU_06925"
     CDS             5500..6024
                     /locus_tag="AC16FU_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994140.1"
                     /translation="MNPRLVRLAKLAALVALAYVLKLPFLSIPNVEFFTYLCFAAGYL
                     FGIWSGALTAAAAMTLYSLFNPYGPAGPILTAAKVLAMTFVGIAGGLTFHLRFVPRAT
                     ILSLAAAALLGLVLTLQFDLITNLAIVVTTGQFWPVMAAAVPFAAIHIGSNTVLFVLF
                     NPLLFRLAKLEHRL"
     gene            6144..>7248
                     /locus_tag="AC16FU_06930"
     CDS             6144..>7248
                     /locus_tag="AC16FU_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2994141.1"
                     /translation="MATTVSDSLVRDSLGAQVRDSLVKENAVRAAQELGAAEITAIVP
                     QDFGDLLRRFGQFDAAGSGPFGQPKQLILWGVPVREAGEIWNGARLFSALQFPQTGTV
                     DLHALPLEEIESLSVGESGNETKSETVETKGVATPENAPYARARLKRGPKGLSVLSVV
                     FTRQLGRAGNFSFSGGFKKARGKDDPRSYDGKSFNGHYRNRFGNGWSLAGDYHLYDEK
                     RGVVAWEELAFLDLRRKAKAEHLSLTLIRKWHERRRLATELFYSRDRVTLKDNAGRFA
                     NGYQQKRPGGALSWQENLSGSQQITGTSRFFYDFFEQDKTQRLYHVVWTAQDAWKPNN
                     LITFVLSGSVEKQRFQPLQFYGGISMALAPAKRM"
BASE COUNT         1536 a         2006 c         2117 g         1589 t
ORIGIN      
        1 cgcggagcgc acctttctcc tcaaagcatt tcccctgccc gatcgattcg atccgctgca
       61 cgccattgcc gatcccatcg gcaaggggcc ggaagagtac gaaaaatctt ttctggagag
      121 tgacgaacac ctgcggcgta tatgttcttt agtagtggaa cgcgccaggg aaaagaatgc
      181 gggctaaaca gtcgctgccg tggaagatta ttttctccgc gcttcttccg tggggtgcgt
      241 tgatctgggt ggggcctctg ccggggggtg ccggggtcgg tgggactgag ttggtaccgg
      301 tcggagactc gactttccgg gacagcacca gaaaaggaaa catcccggcc gacccgtccg
      361 gggcggatac cttgccgacc gaaatacccc ccccgctccg gatggtggag aactactcct
      421 tcggcgtggg ggagcggctg gaatacgaca tcacctacgg acttttcact gcggggtcgg
      481 cggtcatgga agtgaaagag ctggccgacg tcaacggcta cccctgctac cacgtcacct
      541 ccacggcgcg ctccaacgcc ctggtctcca aattcttcgc cgtggaagac tacgtccact
      601 cctttgtgga tgcccgcggg gttttcacct ggaggttcga aaagaagatt caggaaggga
      661 agtataagac ccatcgtcag gcactctatg atcagatcaa tcaccgggtg gtaaccgagc
      721 gcaacgacac gctggcggtg gcgccgttcg tgcaggatgc gctctcggct ttgttcttcg
      781 tgcgcaccca aaagctggaa gtgggcaagt cgctctgggt ggaggccttc tccgacggca
      841 agaagtatcc gctggaagtc cgggtgcaca agaaagaaaa gataagggta ccggccgggg
      901 agttccgctg catcgtggtg gagccgcttt tgaaaacctc cggcatcttc gagcacaaag
      961 gccggctcac cgtctggctc accgacgacc gcttgaagct gccggttttg atgaaaagca
     1021 aagtcattgt tgggaacatc gccgccgagt tgaccgccta caccctgggc aggccggagc
     1081 gcttccgacg atgaagtacg aggcgctcga tttagcgcag gttcgaacct actcgatccg
     1141 gaagcgcaag cacctggccc ggcaaaccga ttttgccggg atcccggagc cacacgactc
     1201 catcgggcaa ttcctggcaa tcctgccgca ggttctgaaa gccaagaatt ttgtgagcct
     1261 ggcggacacg ctggccgcgg ccgcgcgcaa gcgtccggtg attgcccttc tcggggcgca
     1321 cgtgatcaaa tgcggtttat cgccggtgat cattgatctc atccgtaacg gccttttgac
     1381 ccacgtggca gtgaccgggg cgggggcgat ccacgacttg gaggtggcgt tcttcggcca
     1441 gacctccgaa gaagttgccg cccatctgca agatggctcc ttcggcatgg tgcgggaaac
     1501 tgcggcgatt ttcaatgccg cggtcgccgc cggcgaggga gcaggattgg gagagcgctt
     1561 ggggtatttc tttctcgttc ggaaaccaaa gttcctccgt tacagtatcc tcgccgccgc
     1621 ggcggaacag aagatgccgg ccacggtcca cgtggccttc ggcaccgaca cgctccacca
     1681 gcaccccggt tttgatccgg ccagtgtcgg gcttttgacc caccatgact tcaaagtgct
     1741 ggcggcctcg gtcgggcgct tgcgccccgg agcggtggtg ctcaatattg gctcggcggt
     1801 ggtgctgccg gaagtcttcc taaaagcgct ggcggtggcg cgaaacatcg agggcgcccg
     1861 gaaagtgaaa ggatttacgg ctgcgaattt cgatatgatc cagcattacc gccctaacgt
     1921 caacgtcgtc gggcggccca ctttgggcgg cggcaccggc tactcgttca ccggtcacca
     1981 cgagattatg ctgccgcttt tggcttgggc agtcaaagcg aaaatgcagc acacaatagg
     2041 cagaacacga aacacgggaa aaagcaaaag atgaaatgcc ccaccccttc tcccggttca
     2101 cggcctaagg actggaaatg ccgcgcttag tggaatgtgt gcccaatttc agcgaaggcc
     2161 gcaggccgga agtcatcgat gcgctccaga gggagatcgc ggcagtgccc ggcgtggcgc
     2221 ttttggaccg cgaaatggat aaagaccaca accgggcggt tttgactttc atcggtgaac
     2281 cggaggcagt caaaaaggcg gctattcggg caattgccaa agcggctgag ttaatcgaca
     2341 tggaagagca taaaggcgaa cacccacgaa ttggcgccac cgacgtcgtg ccgttcgtgc
     2401 cgatttccga agtgacgatg gatgaatgtg ttcaactggc gcaccaactt ggccaggaag
     2461 tgggggagaa gctgggcatt cccgtttatc tctacgaagc ggcggccacg cggcccgatc
     2521 ggatgaattt ggccaatatc cgcaagggag agtacgaagg cctgaaggaa gaaatcggca
     2581 agaatcccga tcgcaagccc gattttggcc cggaacggct gcatccgaga gccggggcaa
     2641 cagtcatcgg agcgcggatg cctttaatcg cctataacat ctacctcggg acgaatgacg
     2701 tatccattgc caaaaacatc gccaaaaaag tacgcgaatc ttcgggcggt ctgcccgcgg
     2761 tcaaagcgct ggggtttgca atcaaggagc ggaacctcgt ccaggtctca atgaacctga
     2821 cgaactacct gaaaactccg attcacgagg cagtcgaagc ggtcaaagcc gaagccggac
     2881 gtttgggcgt gccggtggtc tcaaccgaac tggtaggtct cgcgcccctg gattcctttt
     2941 tggcttcggc tgaatactat ctcaaacttg agcatttcaa gcgcgaccag attctggaaa
     3001 cgaagctctt gagtctgttc tccgaacgac gagaatctct ggccgatttc gttgaccgcg
     3061 tggcttccgc cgagcccacg cccggcggcg ggagcgtggc ggccctctgc gccgctctgg
     3121 ctgccgcctt gacggctatg gtctgccgat tgaccctcgg taagaagaaa tttgccgcgg
     3181 tggagactga aatcaaagaa gttctgcaaa aagcggagca actccgacag gtgacgttcc
     3241 aactgattca aaaggacaca gcggcctttc agcaggttat ggaagggtac aaactgccca
     3301 aagaaaccga aaaagagaaa gagagcagag accgggcaat tgaaaaagct acgctgcagg
     3361 cctcggaaat ccccctggag gtcatgcggc gggggctgga agtgttgcgt ttgagtgatg
     3421 ttgtggcaga gaagggaaat ccgaactccc ggagcgatgc cggcgtggcg gcgcttttgg
     3481 cccgggcggc cacagaaggt gcgtggctga atgtggagat taacctgcaa tcgattcgtg
     3541 atgccgaatt cgtaaagcgg gtgacagagg aaggccagaa gattctgagc caggcccggc
     3601 tgccgcgcgg caatgttcag accgttctgg cggcgaactg atgaaaaaga caaagcggaa
     3661 aggacctgcg atgcgctgtt ttggggtgac aattcttctt tttttcttga gtaacgtccc
     3721 gcaaagccag gcgaaattga gccaggccta cgaagcggga ttaattgagc gcgtaaaaag
     3781 tctgcggccg gctcaaagcg gggttgttct cgctggcgct gagggtttgc cccggccgtt
     3841 gtgcggtacc ccgattgcgc tcgaagcgaa ttataattac gaaaatctct cttctgagac
     3901 tcaatccgtc ttggcgcaat atctggcgcg accaaccacg ggggtcactc aaatcacacc
     3961 cctgggattt atacgggtcc actatgatac tgcttctggc gatgtggacc gcgtaccctc
     4021 agccgacgtg ctgccgctaa acggcattcc cgatttcatc gacgacgcga tgacggtgct
     4081 tgattcggtc tggctgcgcg aaatagttga tttagggtat cggaaaccac tggtggatgg
     4141 taccgccggc ggagatagtt tgctcgacgt ctactttcag caactggggc ccagctacct
     4201 agggcagacg ttcgtcgatg tcggcaacct cggtccgcag cgcaatcagg gaaccgctta
     4261 cgtggagctg gataacgact acgtgtttcc gcagtatccg acgccggcgg cgcgtcagcg
     4321 catcctgcgg gtgacagcgg cccacgagtt cttccatgcg gtgcaatttg cctacgacat
     4381 ccgcgagttt gagttcacag atccgggcaa ctcaagcacc ttccggccgt actggtacga
     4441 gatgagttcc acttggatgg aagacatcgt atacgatacc attgacgact accactctct
     4501 ggttaagtac gttcttcgat acccctggct ctcgttccgc actttctctt acaacccaag
     4561 cgacacggcg ggcgtcttcc atccctatgc ccaagcactc tggaaccact atctggtggc
     4621 acgcttcgcc gatactaccc tcatccgcac catctggacc ctctgcggtg cggtcaacgg
     4681 atttaatttc tttccagcgg tgaaccaggc gctctcggct aaaatgtcga gctttttggc
     4741 ttcgttccgg gaatactgta tctggaacta ctataccggg tcccgtgaag taccggggaa
     4801 gtactaccgg ctggaaggag agtcgtttcc cgtcggcgcc ttcgttgaca ccctgccgca
     4861 acagtcggtg atagtcggtg acgcccagcc gcctgtccaa gatctggggt gcacgtacta
     4921 tttcatcgtt cccaatgcaa gtcagcccga ttcgttccaa attttcttta cagtgctaag
     4981 cggagccgtg gcacagtggg acatcgccgg cttcgggcgc gacaccacgg gcgataccgt
     5041 ctttatctct tttatgccag tcggcgctgc ttcgtggctg gcacgctacc cggcctggaa
     5101 gacttttacg gatcaggtcg tactgccgat tgccctccag gaaccgattg atgccacgat
     5161 tggcaactat ggctatcatt acgtctttgg tttccagggt ccgccaccgg ccagcgacca
     5221 gtttagtgat ccgtacccga accccatctt ccttcctcaa caaacaacgg tccgatttaa
     5281 ggtaaatctg gctcgggatg ctcaggtaga attctctgta cacgatctgt cgggtcaggt
     5341 tattcacgaa gaacgaccga agaaatttct ggcgggtgtc gagtccttcc taacctggga
     5401 tggcttgaat gatgagcaac aaatggtggc cagcggcgtt tacctctgcg caatcagggt
     5461 gggagacaag accaaagtgt ttaagctcgc cgttgtgcga tgaatccacg cctcgtccgc
     5521 ctggccaagc tggcggcttt ggtggcttta gcgtatgtgc tcaaacttcc attcctttcg
     5581 attcccaatg tggaattttt cacttacctg tgctttgcgg ccggatatct cttcggcatc
     5641 tggtcgggag cgctgacagc cgcggcagcg atgaccctct attcactttt caacccctac
     5701 ggcccggccg gaccgatctt gacagcggca aaagttctgg caatgacctt cgttggaata
     5761 gcgggaggtt tgacgtttca tttgaggttc gttcctcggg caactattct aagtttagcg
     5821 gctgccgccc ttttggggtt ggttctgacc cttcaatttg atctcatcac caatctggcc
     5881 atcgtcgtga cgaccggcca gttctggccg gtgatggcgg cggcggtgcc ttttgccgcc
     5941 atacacatcg gttcgaatac agttctcttt gtactgttca atccgcttct tttccgtttg
     6001 gcgaagctgg aacaccgctt gtgagttact ttggttcaca cattcgttcc acactccggt
     6061 tattttcttt gcttcttttg atttcaacac tccattctct tttcatctgg tttaatacgg
     6121 cggctctcca cgctcaggag agcgtggcta caactgtctc cgactcgtta gttcgggatt
     6181 ctctgggagc acaggtgcgg gactctctcg tgaaagagaa tgcggtccgg gccgctcagg
     6241 aactgggcgc cgccgaaatc accgctatcg tgccgcagga ttttggggac ctcttgcggc
     6301 gcttcggtca gtttgacgct gcgggtagcg ggccgttcgg tcaacccaag caactcatcc
     6361 tctggggtgt tcccgtgaga gaagccgggg agatctggaa cggcgcgcgc cttttttcgg
     6421 cgctccaatt tccccagacc ggcacggttg acctccacgc gctgcctttg gaagaaattg
     6481 agagtttaag cgttggtgaa tcagggaatg agacaaaaag tgagacggtt gaaaccaaag
     6541 gagtcgctac cccagagaat gcgccgtacg cccgggcacg gttgaagcgg ggaccgaagg
     6601 gactgagtgt tttaagcgtc gttttcaccc gccaactggg cagggccgga aatttttcct
     6661 ttagcggtgg attcaaaaag gcgcggggca aagatgatcc ccgttcctat gacgggaaaa
     6721 gtttcaacgg gcactaccgg aatcgcttcg ggaacgggtg gtctttggcc ggtgattacc
     6781 atctctatga tgaaaagcgg ggcgtcgtcg cctgggagga actggccttc ctcgacctgc
     6841 gccgcaaagc caaagccgag cacctatcgc tgacgcttat ccgtaagtgg cacgagcgcc
     6901 gtaggcttgc cacggaactt ttttacagtc gtgaccgggt gactctgaag gataatgcgg
     6961 ggcgctttgc caacggttac caacagaagc ggccgggtgg cgctctgtcg tggcaggaaa
     7021 acctgtcggg gtctcagcag ataaccggaa cgagccgttt tttctatgat ttctttgagc
     7081 aagataaaac tcagcgtctc taccacgtcg tttggacggc ccaagacgcc tggaaaccaa
     7141 acaatcttat tacctttgtt ctctccggtt ctgtcgagaa acagcgtttc cagccgctgc
     7201 agttctatgg gggaatcagt atggcattgg ctccagcgaa gcgaatgc
//
LOCUS       DBNGZA010000317         3562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1972023, whole genome shotgun sequence.
ACCESSION   DBNGZA010000317 DBNGZA010000000
VERSION     DBNGZA010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1972023"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="AC16FU_06935"
     CDS             <1..1132
                     /locus_tag="AC16FU_06935"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR2994142.1"
                     /translation="VVRESSRQFHCVGADLLDPVGQEVKRIYARMTLLATGGAGQVYR
                     HTTNPAIATGDGVAIAYRAGAPVANLEFMQFHPTALAHPKGNWFLISEAVRGEGAKLR
                     LSSGTAFMEHYHPQAELAPRDIVARAIDSELKKRGEACVFLDATHLAKERLQQRFPMI
                     YERLLELGIDMARDLIPVVPAAHYLCGGVVTDAHGRTALPGLYAAGEVAATGMHGANR
                     LASNSLLEALAFAVFAADDAKSSLSEIPSLPFETENPTLGVDSLTREERVQQEELRLR
                     IRQHMWDDVGIVRNDLRLSQAVAATETFLTEAEALWQPQQVTYEMLELRNLALVAHLV
                     ARSAVLRKESRGLHYNTDHPERDDRHWQKDTLLEPPVRTLVP"
     gene            1129..2664
                     /gene="guaA"
                     /locus_tag="AC16FU_06940"
     CDS             1129..2664
                     /gene="guaA"
                     /locus_tag="AC16FU_06940"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR2994143.1"
                     /translation="MTHQRIVILDFGSQYTQLIARRIREFDVFCEIFPGTAPVGSYAD
                     RNLIGVILSGGPASVAAPDAPHFPEEILRLRKPILGICYGLQLLAHRLGGKVARTHAR
                     EYGRAELRIKTFGKLFAGLQPKSVVWMSHGDTVVKLPPGFAGLATTPQVKFAAFGHAA
                     EKIYAVQFHPEVAHTEGGQRILRNFVLTICGARGDWTVRSFISESTARIQATADSLPV
                     VCGISGGVDSTVCALLIHRAIGKNLIPIFIDNGLLRAGEAAEVQKLFARLKIPLRTVD
                     ASQRFLSALAGVSEPETKRKIIGHVFIETFAHATRQLGEVKFLAQGTLYPDVIESQSV
                     RGPSATIKTHHNVGGLPADLKFQLIEPLRNLFKDEVRAVGRKLGVPAQSLGLHPFPGP
                     GLAVRIIGEVTPERLELLRRADFIFIQELRKAKLYDQIWQAFVVLLPVKTVGVMGDER
                     TYENVCALRAVTSRDGMTADWARLPDAFLARVSARIINEVKGINRVVYDISSKPPATI
                     EWE"
     gene            2661..3461
                     /locus_tag="AC16FU_06945"
     CDS             2661..3461
                     /locus_tag="AC16FU_06945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2994144.1"
                     /translation="MKKRSQTLIERAVPDIFSLEGKVAIVTGGSRGIGRETALLLAAF
                     GAEVVVHYHRKRRAAEEVQRQIWSQGRSAEIYGADCSSYAQCRRLVNFTQKRFGRIDI
                     LVNNAAIWEEAPIHRLTPQQLEKTLAVNLKSIFYCTRLAAPIMMRQKSGSIVNLSSTA
                     GQRGEAFHSHYAATKGAITSLTKSLAAELAPYRIRVNAVAPGWVDTDMSHGALTGRDR
                     KKILGAIPLGRAAQPREIAACIAFVASEAAAFVTGEIFNVNGGAVLCG"
BASE COUNT          671 a         1113 c         1110 g          668 t
ORIGIN      
        1 ggtggtgcgt gagtcgagcc gccagtttca ctgcgtcggc gcagatcttc tggacccggt
       61 cgggcaagag gtcaagcgca tttacgcgcg gatgacgctt ctggcgaccg gcggggccgg
      121 gcaggtctac cggcacacca ccaatccggc gattgcgacc ggcgacgggg tggccatcgc
      181 ctaccgcgcc ggggcaccgg tggccaattt ggagttcatg cagttccatc ccaccgccct
      241 ggcacacccg aaggggaact ggtttctgat ctccgaagcg gtgcggggcg aaggggccaa
      301 actgcggctc tcctccggta ctgcctttat ggaacactac catccccagg cggaactggc
      361 accgcgggac atcgtggcgc gcgccatcga cagtgaacta aaaaaacgcg gggaggcgtg
      421 cgtctttctg gacgcaaccc atcttgccaa agagcggttg cagcaacgct tcccgatgat
      481 ttacgagcgt cttttggagc tggggattga catggcgcgg gatttgattc ccgtggtgcc
      541 ggcggcgcac tacctgtgcg gcggggtggt gaccgacgcc cacgggcgga ccgcgctccc
      601 cggtctctat gccgcgggag aagtggccgc aacgggcatg cacggggcca accggctggc
      661 atccaattcc cttttggagg cactggcctt tgccgttttt gcggctgacg acgccaagag
      721 ttccctttcc gagattccgt ctctcccgtt tgagacagaa aatccgacac ttggggttga
      781 cagccttacc cgggaagagc gagtacaaca ggaggaactg cggctgcgta tacgccagca
      841 catgtgggat gacgtgggta ttgtgcgcaa cgacctgcgt ctgtcacagg cggtagccgc
      901 caccgaaaca ttcctgaccg aagccgaagc gctgtggcag ccgcagcagg tgacgtatga
      961 aatgctcgag ttgcggaatt tggccttagt ggcccacctc gtggcccgtt cggcggtgct
     1021 ccgcaaagaa tcccgcggat tacactacaa caccgatcat cccgagcggg acgatcgcca
     1081 ctggcagaag gatacgctcc tggaaccgcc cgtccggaca ctcgtcccat gacccatcag
     1141 cggatcgtca ttctcgattt cggctcccag tacacccagc tgatcgcccg ccgcatccgg
     1201 gagttcgatg ttttctgcga aattttcccg ggtacggcgc cggtgggttc gtacgccgac
     1261 aggaatctca tcggggtcat cctctccggc ggccccgctt cggtcgccgc tccggacgcc
     1321 ccgcattttc cggaggaaat cctgcggctg aggaaaccga ttctggggat ttgctacggc
     1381 ctccagctcc tggcgcaccg gctggggggg aaagtcgccc gcacccacgc ccgcgagtac
     1441 ggccgggccg aactgcggat taaaaccttc gggaaactgt tcgccggcct gcagcccaaa
     1501 agcgtcgtct ggatgagtca cggcgatacg gtggtcaaac tcccgcccgg gtttgccggc
     1561 ctggccacga cgccacaggt gaagttcgcc gccttcggcc acgcggctga gaaaatctac
     1621 gccgtgcagt tccaccccga ggtggcccac acggaagggg ggcagcgcat cctgcgcaat
     1681 ttcgttctga ccatctgtgg ggcccggggc gactggaccg tccgctcctt catcagcgaa
     1741 agtaccgcgc ggattcaggc gaccgcggac agcctgccgg tggtctgcgg catttcgggc
     1801 ggcgtcgatt ccaccgtctg cgcgctttta atccaccggg cgatcggcaa gaacctcatt
     1861 ccgattttca tcgacaacgg ccttttgcgg gccggcgaag cagccgaagt ccaaaagctc
     1921 tttgcccggt tgaagattcc actgcgcacc gtggatgcgt cgcagcgctt tctttccgcc
     1981 ctggccgggg taagcgagcc ggaaaccaag cgcaagatca tcggccacgt cttcatcgaa
     2041 acgttcgccc acgccacgcg ccagctgggg gaggtgaagt ttctagcgca ggggacgctc
     2101 tatccggacg tgatcgagag ccagagcgtg cgcggaccgt cggccaccat taagacgcac
     2161 cacaacgtcg gcgggctgcc ggcggatctc aaatttcaat taatcgaacc gttgcgcaac
     2221 ctgttcaaag acgaagtacg cgcggtgggg cggaagctag gggtgcccgc gcagtccctg
     2281 ggcctccacc cctttcccgg gcccggcctg gcggtccgga tcatcggaga agtgacgccg
     2341 gaacgcttag aacttttgcg ccgcgccgat ttcatcttca tccaggaact ccggaaggcg
     2401 aagctctacg accagatctg gcaggccttc gtcgtccttt tgcccgtcaa aaccgtgggc
     2461 gtgatggggg atgagcgcac ctacgagaac gtctgcgcgt tgcgggcggt gacgagccgg
     2521 gacggcatga ccgccgattg ggcgcgcctg ccggatgctt ttctggcgcg ggtgtccgcc
     2581 cgaatcatca acgaagtgaa aggcatcaac cgggtggtgt acgacatttc ttccaagccg
     2641 cccgcgacga tcgagtggga atgaaaaaaa gatcgcagac gcttattgag cgagcggtac
     2701 cggacatttt ttcactggag gggaaagtcg ccatcgtcac gggaggatcg cgggggattg
     2761 gacgggaaac cgccctcctc ctggcggcgt tcggcgccga ggtggtggtt cactaccacc
     2821 ggaaacggcg ggctgctgaa gaagtgcaaa ggcagatttg gtcgcagggt agaagcgcgg
     2881 agatctacgg cgccgattgt tcgtcctacg cccaatgccg acggctggtc aacttcaccc
     2941 aaaaacgttt cggccgcatc gatattctgg tcaataacgc cgctatctgg gaagaggccc
     3001 cgattcaccg cctgacgccg caacagttgg agaagacttt ggcggttaat ctcaaaagta
     3061 ttttctactg cacgcggctg gcggctccca tcatgatgcg ccagaaatcc ggctccattg
     3121 tcaatctttc ctccaccgcc ggacagcggg gcgaagcgtt ccactcccac tacgccgcaa
     3181 cgaaaggggc gatcacgagt ttgaccaagt ctttggccgc ggaactggcg ccctaccgca
     3241 tccgggtgaa cgcggtggca ccgggctggg tcgataccga catgagccac ggagcgctca
     3301 caggtcggga ccggaaaaaa attctggggg caattccctt gggacgcgcc gcccagccgc
     3361 gggagatcgc cgcctgtatc gcttttgtgg cttcggaggc ggccgccttc gtcaccggcg
     3421 agattttcaa cgtcaacggc ggtgcggttc tgtgcgggtg aggcacccct tgacagtccc
     3481 cgcacaccgc gtaccttcct ccgtcatgac gccttccatc accgccgccc tggatcccct
     3541 gactgagatt ttttctccga tt
//
LOCUS       DBNGZA010000318         5869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_697022, whole genome shotgun sequence.
ACCESSION   DBNGZA010000318 DBNGZA010000000
VERSION     DBNGZA010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_697022"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC16FU_06950"
     CDS             complement(<1..1091)
                     /locus_tag="AC16FU_06950"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR2994145.1"
                     /translation="MPQVITKERSALHFAERMSRLGTETAFEVLGRARQLEAQGRSVI
                     HLEIGEPDFDTPPNITDAAIVALKQKYTHYTPAAGLPEVREAVARYVAKTRKAPVSAD
                     QVVITPGAKPIITFSLLALIDAGDEVIYPNPGFPIYESVIRFCNGRPVPIRLREERNF
                     RLDSQELADLVTDRTKMIIVNSPENPTGSVLTRRDVEEICEIVRERDIWIFSDEIYSR
                     DLYGGEYTSPYSFPDMPERTIIMDGMSKAYAMTGWRLGYGVMPKELALQVTRLQINCT
                     SCTAAFSQRAIIEALEGPQESVAAMAAEFKRRRDVIVKGLNAIPRLSCQNPDGAFYVF
                     PNVSQIGWPSKKLAAFLLEEGGVACLTGTS"
     gene            complement(1158..2117)
                     /locus_tag="AC16FU_06955"
     CDS             complement(1158..2117)
                     /locus_tag="AC16FU_06955"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR2994146.1"
                     /translation="MEWKEKKKTREGYGRALEELGERNPNVVVLTGDLAESTMAHFFA
                     QKFPERFFNMGVAEQNMANVSAGLSLTGKIPFFSTYGCFASCRALDMLRVTICYSNLN
                     VKIGGAHGGISVGPDGATHQAMEEFSIMRSMPNMHLVVPCDYWEAKKATLAAAEIRGP
                     VYIRFGREDVPVVTTEETPFVFGKGLELRPGHDVALLACGTMVSEALSAAEMLATEGI
                     DARVVNIHTVKPIDQEIIIKAARECGAVVTAEEHQINGGFGGAVAEVLVRNCPVPVEM
                     VAMNDRFGQSGKPEELLAAFHMKDVDIAKAAKKVLARKRNGKM"
     gene            complement(2163..3080)
                     /locus_tag="AC16FU_06960"
     CDS             complement(2163..3080)
                     /locus_tag="AC16FU_06960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR2994147.1"
                     /translation="MNPELLVAEPPPKTERVWMNPFKYSVEQLKEKARIIRRNIITMN
                     VEAKSGHTGGPLSAADFCTVLFFHEMNFDPKSPKWPDRDMWFFSFGHVTPVHYSCLAE
                     AGFFPKCDLMKFRKFDGHLQGHPSSLDTPGVEVSSGSLGQGLSISVGAALGSRLDKHP
                     RRIYCLMSDGEQQEGSIWEAAMAGGHFRLDNLCGIIDYNRKQIDGDVEEVMGVAPLAD
                     KWRAFRWHVIEVDGQDIPQLLAAFDEARRIKGRPTVVLAHTVMGKGVSFMEDDFRWHG
                     KPPKPDEGERALAELGTTYAEWSRYLLEN"
     gene            complement(3314..3922)
                     /gene="coaE"
                     /locus_tag="AC16FU_06965"
     CDS             complement(3314..3922)
                     /gene="coaE"
                     /locus_tag="AC16FU_06965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR2994148.1"
                     /translation="MILGLAGGIASGKTEVARVFQKLGAIIISGDDLGREVVEKDQRV
                     WVRLVETFGRKILNSNGTLNRRKLGQLAFSSPVSRKKLNRIVHPSLLRLMRQRVTYVR
                     RQNRKNPIVIDAALLVEWKIPVKMDKLIVVYASRNIRVRRLRKKDLTLSEIRDRMRSQ
                     LPFSVERKHADIIIRNDGTLEELRQKAREVWNQLIRSERGRH"
     gene            complement(3937..>5869)
                     /gene="polA"
                     /locus_tag="AC16FU_06970"
     CDS             complement(3937..>5869)
                     /gene="polA"
                     /locus_tag="AC16FU_06970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR2994149.1"
                     /translation="KLDLKHLRHQPPDEAALRRLFLDLEFTSLLKILGGEEDGKKVTY
                     RLIGDMAELKSLLAALKKAGEFAVDTETTSLEPLNCELVGISVAKKAGEAFYIPVGHI
                     GPDEKKNLSLKETLALFRSVLETDEPKKSGQNVKYDYQVFRNYAVEMKGIACDTMLVS
                     YLLNPTGRQHNLDALAQEHFGHKMIPISDLIGTGKKQRSFAEVPVEKACEYSAEDADF
                     TFRLKEKLLPRLAELGLEKLFYEIELPLVPVLAAMERNGVALDVPFLKKLSIELDKRL
                     EKLTEKIYDLAGEKFNINSTQQLGQVLFEKLKLTPGRKTTKKTALSTDIGVLEELAKE
                     HKLPALIVEYRQYTKLKSTYIDALPALINPRTGRLHTSFNQAVAATGRLSSADPNLQN
                     IPIRTEVGSQIRKAFIPGEKGYSILSADYSQIELRLMAHFSGDKNLIAAFERGEDVHA
                     RTASEVFGVPIDKVTSEQRRVAKTANFALMYGVTAYGLSQQSEMTLQEAKQFIETYFA
                     RYPGVRQYMDDAIAQAQEKGYAETLFGRRRSLPEINSQNAQVRGFAERNAINTPLQGT
                     AADVIKKAMIEVHERLKGKKSKMILQVHDELVFEVPPDEVEWLKKMVTKEMEEVVKLK
                     VPLKVDIGVGKNWLETKEG"
BASE COUNT         1188 a         1679 c         1623 g         1379 t
ORIGIN      
        1 gaggtgcccg tcaaacaggc gacgccccct tcttcgagga ggaaagcggc taatttcttg
       61 ctcggccacc cgatttggga aacgttgggg aaaacgtaga aggcgccgtc gggattctgg
      121 caactgaggc ggggaatggc gttgagtcct ttgacaatca catcgcgtcg gcgtttgaat
      181 tcggccgcca tggcggcgac cgattcctgc ggcccttcca gcgcttctat gatggcgcgc
      241 tgggaaaaag ccgcggtgca ggaagtgcag ttgatttgca ggcgcgtcac ctgcagggcc
      301 aactctttcg gcatgacgcc gtaccccagc cgccagccgg tcatggcgta ggctttcgac
      361 atcccgtcca tgatgatcgt ccgttcgggc atatcgggga aagagtaagg ggaggtgtac
      421 tcgccgccgt agaggtcgcg gctgtagatt tcatcggaaa aaatccagat gtcgcgttcg
      481 cggacaattt cgcagatttc ctcgacatcg cgccgggtca ggacgctgcc ggtagggttt
      541 tccggggagt tgacgatgat catcttcgtc cggtcggtga cgagatcggc taactcctgt
      601 gagtcgagtc ggaaatttcg ctcctcgcgc aggcggatcg gcacgggccg gccgttgcag
      661 aaacgaatga ccgactcgta gatgggaaag ccgggattgg gatagatgac ctcatcgccg
      721 gcgtcgatga gcgccagaag cgaaaaggtg ataatcggct tggctcccgg ggttatcacc
      781 acctggtcgg cggagacggg cgccttgcgc gttttggcca cgtaccgtgc caccgcctcg
      841 cgcacttcgg gcaggccggc agcgggcgtg taatgggtgt atttctgttt cagtgctaca
      901 attgctgcgt cagtgatatt agggggtgta tcaaaatccg gctcgccaat ttctaagtga
      961 atgactgagc gcccctgcgc ttctaactgc cgggcccggc ccaggacttc aaaagcggtc
     1021 tcggtcccga ggcgggacat ccgttcggcg aagtgaagcg ctgatctttc tttggtgatt
     1081 acttgtggca tatcacatta tattccaatt gattactgaa atttgtaaaa gcgactcttt
     1141 aagtcccaac caaagggtta catttttcca ttccgcttac gggccaaaac tttcttcgcc
     1201 gcttttgcga tgtcgacatc tttcatgtga aaagcggcca agagttcttc cggttttccc
     1261 gactggccga agcggtcgtt catcgccacc atttccaccg gcaccggaca gttgcggacg
     1321 agcacttcgg ctaccgcgcc gccgaagccg ccgttgatct ggtgctcttc ggcggtgacg
     1381 acggccccgc actcccgcgc ggccttgatg atgatttcct ggtcaatcgg cttgaccgtg
     1441 tgaatgttca ccacgcgagc atcgatgccc tctgttgcta acatttccgc ggcggaaagt
     1501 gcttcggaaa ccatcgtccc gcaggccaga agtgccacgt catggccggg ccggagttcc
     1561 aagcctttgc cgaaaacaaa cggcgtctct tccgtggtga cgaccggcac atcttcccgg
     1621 ccaaagcgga tgtaaaccgg tccccgaatt tccgcggcgg ccagggttgc tttcttcgcc
     1681 tcccagtaat cacaaggcac caccaggtgc atattgggca ttgagcgcat gatggagaat
     1741 tcttccatcg cctggtgagt ggcgccgtcc ggacccacgg agatgccgcc gtgcgccccg
     1801 ccgatcttga cgttcaagtt cgagtagcaa atcgtgaccc ggagcatatc gagcgcccgg
     1861 caggaagcaa agcagccgta ggtggagaag aaagggattt tcccggtcaa agagagtccg
     1921 gcggagacgt tggccatatt ctgctccgcc acccccatgt tgaagaagcg ctccgggaat
     1981 ttctgggcga agaagtgggc catggtcgat tcggccaggt cgccggtgag caccaccacg
     2041 ttggggttgc gctcgcccaa ttcttccaac gcccgtccgt atccttcccg cgtctttttc
     2101 ttttctttcc actccaccgt tttatccctc ggtcctcatc cgtctcaggg gcggactgaa
     2161 ttttaatttt ctaagaggta gcgcgaccac tccgcatacg tcgttcccag ttcggccagc
     2221 gcccgttcgc cctcatccgg tttgggcggt ttgccgtgcc agcggaagtc gtcctccata
     2281 aaggaaacgc ctttccccat taccgtgtgg gccagaacca ccgtaggccg gcctttgatc
     2341 cgccgggctt cgtcgaacgc cgccaaaagc tgcggaatgt cttggccatc gacttcaatc
     2401 acgtgccagc ggaaagcgcg ccatttgtcg gctaaaggcg ccacgcccat tacctcctcc
     2461 acgtcgccgt cgatttgctt gcggttgtag tcgatgatcc cgcagaggtt gtccagccga
     2521 aagtgtcccc cagccatcgc cgcttcccag atcgagcctt cctgctgttc gccgtcggac
     2581 atcaagcagt aaatccggcg cgggtgctta tccaagcggg agccgagcgc cgcgcccaca
     2641 gaaatcgaaa gcccctgacc cagcgacccg gaggaaactt ccacgcccgg agtgtccaga
     2701 gaggagggat gcccctgcag gtggccgtca aatttgcgga acttcatcag gtcgcatttc
     2761 gggaaaaagc ccgcttcagc cagacaggag tagtgcaccg gtgtgacgtg gccgaaggag
     2821 aaaaaccaca tgtcgcggtc gggccacttg gggcttttcg gatcgaagtt catctcgtgg
     2881 aagaagagca ccgtgcagaa gtcggctgcc gaaagcggcc cgccggtgtg ccccgacttg
     2941 gcttccacgt tcatggtaat gatgttccgc cggatgattc tggctttttc ttttagctgt
     3001 tcgacggaat acttgaaggg attcatccag acccgttcgg tcttgggcgg tggctcggct
     3061 accaacagtt ccggattcat tcagtctcgc ttgtgaaaag tgtcacattc actaccaata
     3121 tacgccaggc gaaaagaaat gcaaaaggaa tatttgtctt aaactacctc ctggtggcgc
     3181 ggaatggttc tcatattccg cgccgcgcag gttgcggttc gacttcgctc accgcgagcc
     3241 aaaacccttc ggccccgctc agggcaggcc agcctgtgcc tccctatttg aaaaacccca
     3301 cgtaggcggc actttagtgc cgccctcttt ctgaacgaat caactgattc caaacctctc
     3361 gggctttctg tcgcagctct tccaatgtcc catcgttgcg aatgataatg tcggcgtgct
     3421 tccgctcaac cgaaaaaggc aattgtgagc gcatacggtc acgaatttca gaaagggtta
     3481 ggtccttttt tctgagacga cggacgcgga tgttccggga ggcgtagacc acaatcagtt
     3541 tgtccatttt gaccgggatt ttccactcga ctaagagtgc cgcgtcaata acgataggat
     3601 tcttccgatt ttgcctccgc acgtacgtta cacgttggcg catcaaccgc aagagggacg
     3661 ggtgcacaat ccggttcagt tttttgcggc tgaccggaga agaaaaagcc agttggccta
     3721 acttccggcg gttaagcgtg ccattggaat tgagaatttt ccgaccaaac gtttcaacca
     3781 aacgcaccca tacacgctgg tctttttcca ccacttcccg gcccaagtca tcgccggaaa
     3841 tgatgattgc accgagtttc tggaagaccc gggcgacttc agttttgccg gaggcgatgc
     3901 cccccgccag gccgaggatc atggagtcag gggaaattac ccttccttcg tctccaacca
     3961 gttcttgcct acgccaatat cgacctttag cggcactttc aatttgacga cctcttccat
     4021 ttccttcgtc accattttct tgagccactc cacctcatct ggtggcactt caaagaccaa
     4081 ctcgtcatgg acttgcaaga tcatttttga ttttttgcct ttcagtcgct cgtgcacttc
     4141 gatcatcgct ttcttgatga catccgccgc ggtgccctgc aggggggtgt tgatggcgtt
     4201 ccgctcggca aagccgcgca cctgtgcgtt ctgggaattg atctccggca gggaacgccg
     4261 ccggccgaag agcgtctccg cgtacccttt ctcctgcgcc tgggcgatgg cgtcgtccat
     4321 atactggcgt acccccgggt agcgggcaaa ataggtttcg atgaactgct tggcttcctg
     4381 cagggtcatc tcactctgct gggatagacc gtaagcggtc accccgtaca tcagggcaaa
     4441 attggcggtt ttggccaccc gccgctgttc ggaggtaact ttatcaatcg gcacaccgaa
     4501 cacctccgag gccgtgcggg cgtggacgtc ttcgccccgc tcaaaggcgg cgatgaggtt
     4561 cttatcgccg gagaaatggg ccattaaccg gagttcgatc tgagaatagt cagccgagag
     4621 aatggagtac cctttctcac cgggaatgaa cgccttgcgg atttgactgc cgacttcggt
     4681 gcgaatcgga atgttctgca agttgggatc ggctgaggaa agccgtccgg tagcggccac
     4741 cgcctgattg aacgaggtgt ggagtcgccc ggtccgggga ttgatgagtg cggggagggc
     4801 atcgatgtag gtcgatttca atttcgtata ttgccgatac tccacgatca acgcgggcag
     4861 tttgtgttct ttggccagtt cttctaaaac cccaatgtcg gtggagagtg cggttttctt
     4921 agtggtcttc cgccccggcg tcagctttaa cttctcaaac aaaacctgac ccagttgctg
     4981 ggtggaattg atgttaaact tctctccggc caggtcatag attttctccg tcaatttctc
     5041 caaacgttta tccaactcga tggagagttt tttgagaaag ggcacatcca gggccacacc
     5101 gttccgctcc attgcggcca gcaccggcac gagcggcagt tcaatctcgt agaaaagttt
     5161 ttccaatccc aattcggcca ggcgcggaag gagtttctct ttcaaccgga aggtgaaatc
     5221 ggcgtcttca gcggaatatt cacaggcctt ctcaaccggc acctcggcga aggatctctg
     5281 ctttttcccc gtcccgatca aatcggaaat gggaatcatc ttatggccga agtgttcctg
     5341 agccagtgcg tccagattgt gctgcctacc tgtggggttc agcaggtagg agaccagcat
     5401 cgtgtcgcag gcaatgcctt tcatctcaac agcgtagttc cgaaaaacct ggtagtcgta
     5461 ctttacattc tgtccgcttt tctttggttc gtcggtctcc aaaactgaac gaaaaagagc
     5521 cagcgtttcc ttcagcgaca ggtttttctt ctcatcgggt ccgatgtgcc cgacgggaat
     5581 atagaaggcc tcgcccgctt ttttggcgac tgagatgcca accaactcgc aattgagcgg
     5641 ttccagactc gtggtttcgg tatcgacggc gaactctccg gctttcttca atgcggccag
     5701 caagctcttg agttccgcca tatcaccgat caagcggtag gtgacttttt tcccatcctc
     5761 ctccccgccc agaatcttca aaagcgaggt gaattcaaga tcaaggaaga gtcttcgcag
     5821 cgcggcttca tccggcggct gatgacggag atgttttagg tcgagtttg
//
LOCUS       DBNGZA010000319         3672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_165209, whole genome shotgun sequence.
ACCESSION   DBNGZA010000319 DBNGZA010000000
VERSION     DBNGZA010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_165209"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..562)
                     /locus_tag="AC16FU_06975"
     CDS             complement(53..562)
                     /locus_tag="AC16FU_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(380..382),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR2994150.1"
                     /translation="MRVAGESQRQEGLVPTHLKAGDSAPEFTLTSHTGDKVSLSDFKD
                     KKNVVLTFYVLSWTGVUTQQMSGYRDKKTRFDGFNAQVLGVSVDSRFCQNAWAKSLGG
                     LPFPLLSDLWPHGATHQKYGVFNEKDGLGERTTFVIDKIGKIVFIDENSRPQLPDMDK
                     LLAALEKLK"
     gene            complement(630..1742)
                     /locus_tag="AC16FU_06980"
     CDS             complement(630..1742)
                     /locus_tag="AC16FU_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HIR2994151.1"
                     /translation="MRKYLEKVQNFRLLPVFVAVSMAIGITIGKIYGISNFQLTPPID
                     AVKSIFQGTYQFSLSNSLALGVVLGLFLMIYPAMTKVKIEDLGQAARSPKQLLIVLFF
                     NFAIAPFLMLFFARLFLSGSQDLYVGLVLYGLAPCIAMVIVFTFLALGNNALAIVLVA
                     INSVAQMILIPVYAKLLLGNITFDVFVVGGSVLLYLGLPLLAGFFTRRIAVRKLGEDG
                     FKSFQTYLNSLSIVGLLFTLVIMFALKGDLILQKPLIILQMAIPMTLFFWTSFGIVYL
                     VSWKLGLDYQDSVACAFNSTGRDFEIAIAIAITAFNPAVALATVVGPLIEVPVMLVLV
                     WAARRSRAKLFREVHLSAGEAGEPAVPPLTPAGFVK"
     gene            complement(1914..2690)
                     /gene="lipB"
                     /locus_tag="AC16FU_06985"
     CDS             complement(1914..2690)
                     /gene="lipB"
                     /locus_tag="AC16FU_06985"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR2994152.1"
                     /translation="MAKAEFGAVREIGVSVHQGQLRVVDVGVVPYGQALDVQLRLVEA
                     RRLGEIPDTLLFVEHPPVVTLGRAAKATSLLTPKDHLAKQGVELFEITRGGDVTFHGP
                     GQVVGYPIIDLNARFDSVHRKRGRDLHQYLRDLEEVLLQTLAAFGLKGERQAGFTGAW
                     VNNRKIVAIGVAVKHWISYHGFAFNVNVDLNYFNLIVPCGLAGKEVTSLERELGRPVS
                     MEETKANLSKAFAQVFEKEIVSKAGEELAERVKSPSLLTA"
     gene            complement(2742..3014)
                     /locus_tag="AC16FU_06990"
     CDS             complement(2742..3014)
                     /locus_tag="AC16FU_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994153.1"
                     /translation="MAEVTAAHAAAVAQATRASGAIVKVAPEEFQRLVGRTESPFVVT
                     AYGGWPSKRHQYLTNYRGLFLYCKSEKPLQLPTSAEVVQAKSIWVP"
     gene            complement(3075..3488)
                     /locus_tag="AC16FU_06995"
     CDS             complement(3075..3488)
                     /locus_tag="AC16FU_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2994154.1"
                     /translation="MPNQAGFFTKLDTVIVRVRDLEKARLWYEEKLGLKAAFIDETEK
                     LAVLGTGGTTRLTIYQLKPREKLVTPPLAFEGSAGGERVAGTYPILCAEDIKATYELL
                     KSRGVAVEPEIHSGGGGQWFAFADLDGNKMEVCRF"
BASE COUNT          800 a         1084 c          949 g          839 t
ORIGIN      
        1 tagcacgtct gtctcctccc gattcacctg aaccgctgga attgacatgg ttttacttca
       61 atttctccaa tgccgcgagg agtttatcca tatccggcag ctgcggcctg gagttttcat
      121 caatgaaaac gattttccca atcttgtcga tgacgaaggt cgtccgttcg cccaagccgt
      181 ccttctcatt gaaaacgcca tacttctggt gagttgcgcc gtgaggccag aggtcggaga
      241 gcaaaggaaa aggcagcccg cccaaactct tggcccacgc attctggcaa aatcggctgt
      301 ccaccgagac gcccaggacc tgggcgttga atccgtcgaa tcgggtcttc ttgtcccgat
      361 agccggacat ttgctgcgtt caaaccccgg tccaggagag aacgtagaac gtcaggacga
      421 cgttcttttt gtccttgaaa tcggagagac tgaccttgtc gccggtatgg gaggtgagcg
      481 taaactccgg tgcgctgtcg cctgctttca ggtgtgtcgg cacaagtccc tcctgtcttt
      541 gagattcacc cgcaacacgc attgaatgcc ggtcacgtaa atttcggcgg ttgcaccggc
      601 atttgatcct tctaatatac ggccggaaat tattttacga aacccgccgg cgtcagtggc
      661 ggaaccgccg gctctcctgc ttcgcccgcg ctaaggtgta cttcccgaaa taacttcgcc
      721 cgggaacgcc tggcggccca gactaaaacc agcatcaccg gaacctcaat caaaggtccg
      781 accacggtgg ccagggccac cgctggatta aatgccgtga tcgcaatggc aatggctatc
      841 tcaaaatctc ttccggtaga attaaaggcg caggctacgg aatcttggta atccagaccg
      901 agtttccagg agaccagata aactatcccg aagctcgtcc agaaaaagag cgtcatcggg
      961 attgccatct gaagaataat cagcggcttt tgcaaaatga ggtccccttt cagggcgaac
     1021 ataatgacca gggtgaaaag caggcccacg atggaaagag aatttaggta ggtctggaag
     1081 ctcttgaagc cgtcttcacc caatttcctg actgcgatcc ggcgcgtgaa aaagcctgcc
     1141 aaaagaggca ggccgagata caaaagaaca ctcccgccca cgacgaaaac gtcaaaggtg
     1201 atattcccca gtaaaagttt tgcgtaaacc ggaatgagta tcatctgcgc gacagaattt
     1261 atggccacga ggacgatggc cagtgcgttg ttccccaaag ccaagaaggt gaacacgatg
     1321 accatcgcaa tgcacggggc cagtccgtag agcaccaggc cgacgtagag gtcttgactc
     1381 ccggaaagga acaacctggc gaaaaacagc atcaggaaag gagcgatggc aaaattgaaa
     1441 aataatacga tgagcagttg tttgggagag cgggcggcct ggcccagatc ttctattttg
     1501 actttggtca tggccgggta gatcatgagg aaaagtccga gcacaacccc cagggccagg
     1561 ctgttggaaa gactgaactg gtaggttccc tgaaatatcg atttgacggc atcgatcggc
     1621 ggcgtcagtt gaaagtttga aattccgtat attttgccga tggtaattcc gatggccatg
     1681 ctgacggcaa cgaataccgg caggagcctg aagttctgaa ccttttctaa gtatttcctc
     1741 attgcaattg ctgcctaaga atgagaatat gaactggtgc cggtgagcga cccggccctt
     1801 ttccggagaa tccttgaaat ttccttttct ccgtgtcctc tcgaagagtc gagtctccga
     1861 ccgtgtctcc gtggtgaaaa aaaactcgcg tgggcgcggc gcccccgccg cgcttaggct
     1921 gtcagcaggg agggtgactt gacccgctcc gctaactcct ctcccgcttt ggacacaatc
     1981 tctttctcga atacctgcgc aaacgccttg cttaagtttg ccttcgtttc ttccatagat
     2041 accggtcgcc ccaattcccg ctccagggac gttacttcct tcccggccag cccgcagggg
     2101 acgatgagat tgaagtagtt gagatcgacg ttcacattga acgcaaaccc gtggtaggag
     2161 atccaatgtt tgacggccac gccgatggcg acaatcttcc ggttgttgac ccacgctccg
     2221 gtgaacccgg cctgccgttc ccctttcagt ccgaaggcgg cgagcgtctg caggagcacc
     2281 tcttcgaggt cccgcagata ctggtgtaaa tctcttcccc gcttgcggtg aacggagtcg
     2341 aaccgcgcat tgaggtcgat gatcgggtag cccaccacct gccccggccc gtggaaggtg
     2401 acgtctccac cgcgggtgat ttcaaaaagt tcgactccct gctttgccag atggtcttta
     2461 ggagtaagaa gactcgtggc cttcgccgcc cgccccagag tgacgaccgg cggatgctcc
     2521 acgaagagga gggtatcggg aatttctccc agccgccgcg cttccaccag ccgcaactgc
     2581 acatccagtg cctgtccgta gggtaccacg cccacgtcca cgacccgcaa ctgaccctgg
     2641 tgaacggaga ctccgatttc tcgcactgct ccaaactcgg ctttggccat tcgtgtttgc
     2701 tttccctgtg ttctgtctcc cgtattccgt gttccgcctt cctacggcac ccagatgctt
     2761 ttcgcctgca ccacttccgc gctcgtgggc aattgcaacg gcttttcgct cttgcagtag
     2821 aggaagagcc cccggtagtt ggtcaggtac tggtgccttt tcgacggcca cccgccgtaa
     2881 gccgtgacga caaaaggact ctccgtgcga ccgactaacc gctggaattc ttccggcgcc
     2941 acttttacaa ttgctcccga cgcccgcgtt gcctgcgcca ccgccgctgc gtgtgctgct
     3001 gttacttccg ccaccgctcc tccgcccatt gtgtctttcc ccgtgtcctg tatgctgtat
     3061 tctgtgttct gctctcagaa gcggcacact tccatcttat tgccgtccaa atcggcgaac
     3121 gcaaaccact gcccgcctcc gccggagtgg atttccggtt ccaccgccac tccccggctc
     3181 ttcaggagtt catacgtcgc tttgatgtct tcggcgcaca aaatcgggta cgtccccgcc
     3241 acccgctctc ctccggctga accttcgaac gccaggggcg gtgtgactaa cttctccctc
     3301 ggcttcaact ggtagatggt caaacgtgtt gtgccgccag tgcccagaac cgctaacttt
     3361 tcagtctcat cgatgaaggc ggccttgagc cccaactttt cctcgtacca gagccgggcc
     3421 ttctcgagat cgcgcacccg tacgatcacc gtatcgagtt tggtaaaaaa tcccgcttga
     3481 tttggcaaat atcaccccgc gcagagagat caaaatttga aaattatcgg cagtttgttc
     3541 ggtaagattc actgaacatc aatgtagtca gctgcggcct gcggctccgg tatcggcagg
     3601 tgatcttcgc gtagaccttg caggtgcaga gcaattgctt cggacatgtt ctgctccacc
     3661 tcttcccgcg tc
//
LOCUS       DBNGZA010000320         1244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_165211, whole genome shotgun sequence.
ACCESSION   DBNGZA010000320 DBNGZA010000000
VERSION     DBNGZA010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_165211"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..409)
                     /locus_tag="AC16FU_07000"
     CDS             complement(107..409)
                     /locus_tag="AC16FU_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994155.1"
                     /translation="MQAADASPGQKKKTSDCWFGGDKVQHALVSTYLTFFIYQVNREI
                     FDEKKSEARVVAVTTTFFIGTAKETYDRTVRRTRFSLKDLVADGVGIAFGLWATTW"
     gene            complement(547..>1244)
                     /locus_tag="AC16FU_07005"
     CDS             complement(547..>1244)
                     /locus_tag="AC16FU_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994156.1"
                     /translation="EFKVSVGQKGDFPTAFDRFARPDSVAGLNYRESGNANLAVGRTK
                     EIEGSVTWRKSAFAVGTTGTYQWLDKKILWQNRDPNLQYGDWRPNNEDVKTWGFSTFI
                     SGTLPRWGSAYLVYGYKNPVIVQTEASLPLAPKHRVYFQVWNAERPIENVLTVQGELD
                     GEFLSSRKDDLYPGNRLPAVLRLDFKARFKIKDLTIYYFFENLTNRPYTLRGITPAVE
                     RGHSFGFAWEFFD"
BASE COUNT          288 a          341 c          345 g          270 t
ORIGIN      
        1 ccattgggaa aagtagagac tgataatgtt cattgagaaa attgtgttag caaagtcgta
       61 agcagcccag ccgaagatcg tcaaacggtc gcgtccggac atgcgcttac caggtagtcg
      121 cccagaggcc gaaggcgatc cccacgccgt cggccaccag gtctttcaga ctgaagcgcg
      181 tcctgcggac ggtccggtcg taggtttcct tggcggttcc gatgaaaaat gtggtggtga
      241 ccgccacgac ccgcgcctcc gacttttttt catcgaaaat ttcccgattg acctggtaga
      301 tgaaaaacgt caggtaggtg ctgaccagcg cgtgctggac tttatccccg ccgaaccagc
      361 aatcagaagt tttcttcttt tggcccggag aggcgtcggc cgcctgcata gaaggactga
      421 agcgggaaaa cgtaagttcg aaagtattcg gtagagaata gaaagaagga gacggctgca
      481 gagcgggagc aacgtctcga ggcacttggc ccaacagacc gagcaccacc aggcacgccg
      541 ctcccatcaa tcgaaaaact cccacgcgaa accgaaacta tggccgcgct cgactgcagg
      601 cgttatcccg cgcagggtgt agggccggtt ggtgaggttc tcaaagaagt agtatatggt
      661 caggtctttg attttgaacc gggctttgaa atcgaggcgc aaaacagcgg gaagccggtt
      721 gcccgggtac agatcatctt tccgcgagga aaggaattca ccatccagtt ccccctgcac
      781 ggtcaggaca ttttcaatcg gccgctccgc gttccacacc tggaaataga cccggtgctt
      841 cggagccaag ggtaatgacg cttcggtttg aacaataacc ggattcttgt acccgtagac
      901 taagtaggcg cttccccagc ggggcagcgt tccgctaata aaggtcgaga agccccaggt
      961 tttcacatcc tcgttattgg gtcgccagtc tccgtattgc aggttcggat ctctgttttg
     1021 ccagaggatt tttttgtcta accactggta agtcccggtg gttccgaccg caaatgcaga
     1081 tttcctccac gtaacggatc cctcaatttc cttcgttcgc cccacggcca ggttggcatt
     1141 gcccgactcc cggtagttga ggcccgccac cgagtcgggt cgtgcaaaac ggtcgaaggc
     1201 cgtcggaaaa tcgcccttct gtccgacgga aactttgaat tcag
//
LOCUS       DBNGZA010000321         4013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_379723, whole genome shotgun sequence.
ACCESSION   DBNGZA010000321 DBNGZA010000000
VERSION     DBNGZA010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_379723"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="AC16FU_07010"
     CDS             complement(<1..881)
                     /locus_tag="AC16FU_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2994157.1"
                     /translation="MIEKIIAYSAKNRFIIILLFILVVSWGIWALFRTPLDAIPDLSD
                     NQVIVFTEWPGRSPKVIEDQITYPLASNLQGLPHLKVVRASSAFGFSMIYVIFEDNVD
                     IYWARTRVLERLNYAQNLLPPGVVPTLGPDGTGVGHVFWYTVEGDGYDLGTLRALQDW
                     YIRYQLNSVPGVAEVASIGGFVKQYQVDLDPQKLLAYHVPVKEVLNAIKMSNQDVGGR
                     LVEATDMEYVVRGLGYIQSTTDIENVVVGATKEGVPIYVRNIGTVQLGGDIRRGVLDK
                     NGVGEVVGGIIVMRYQE"
     gene            complement(1088..2362)
                     /locus_tag="AC16FU_07015"
     CDS             complement(1088..2362)
                     /locus_tag="AC16FU_07015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994158.1"
                     /translation="MKRIIIGIAVLAAAVLATFYLTRSAGRKAVRQVTAQAEEKEIYV
                     CPMHPMVTSDKPGNCSICGMDLVKRSSSEELTLTAQEELVAHEVRLSPTQQVLANVAT
                     EAVAVRLLAKEITTVGTIDYVEGNIANLSARVAGRTDSLFVNYTGERVTRGQPLLSMY
                     SPDIVGAEQEYLLALDSNNPALLESARRKLALWGITAEQIDRLGQSREMRDHLTIFSP
                     ISGTVIDKLVHLDMYVAAGQPLYNIADLSTVWMWAEIYEYEFANVRVGQSVEVTAVAY
                     PGEKFYGTINFVSPVVDTLTRTVRIRAEFDNPSDRLKPGMYVDARIKVNLGRKLAIPA
                     SAVLNTGQRQVAWVEKEPGVYTPRDVQIGQRAGNYCEVLSGVAAGEKVVTQAGFLIDS
                     ESQLARLSGAHAGHDTTSGKKQKEAMPGMPGM"
     gene            complement(2391..3698)
                     /locus_tag="AC16FU_07020"
     CDS             complement(2391..3698)
                     /locus_tag="AC16FU_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994159.1"
                     /translation="MRRIRSGLKGSFLLFSCLAIGFFPGVSPAQDTTRTPLAQLVAEA
                     VRVNPNLRVAARNIEKAQAEVARVGYLPDPELMLGVENLPTSFSFREDEMTMKSIGIS
                     QMFPFFGKLGTKKNVVRQAVEVARWNYEEQKLELIRQVKETYFELFAQRRSQELTQKA
                     ITILSDFVALTRTRYEVGTGLQQDLLMATVEQARLEEKLANLEKRTVAVQNELKSLLD
                     RGPETPDIIPEEVKPVLYTPNRDSLLAGLTETRPMLKMLRADILKAQAEYKAAKREAW
                     PDFTWSVAYGIREGLRTPGMPGQDLDNMVTARLGLNLPINSSRRQGKEAAQASLELTN
                     AVSLLTHHAKHDVPLRIDDYLTEIDRNGRLILLYEKTILPQGQLAVQAAQVNYQVGKI
                     DVLTWLSNQLNLLDYQIDYFMTVSEYQKSIARLEAETGVTLQK"
BASE COUNT          780 a         1217 c         1117 g          899 t
ORIGIN      
        1 ttctcctggt agcgcatgac gatgatgccg cccaccactt cgcccacccc gtttttgtcc
       61 aaaacgccgc ggcggatgtc gccgcccaac tgcaccgtgc cgatgttgcg cacgtagatc
      121 ggcacgcctt ctttcgtggc acccaccacg acgttttcaa tgtccgtggt gctctgaatg
      181 taccccaggc cccgcaccac gtactccatg tcggtggcct ccaccagccg cccccccaca
      241 tcctggttgg acatcttgat ggcgttcagg acttctttca ccggcacgtg gtaggccaga
      301 agtttctgcg ggtctaaatc gacctggtac tgtttgacga atcctccgat ggaggctact
      361 tcggccaccc ctggaacaga attcaattga tagcggatgt accagtcctg gagtgcccgc
      421 agcgtcccca aatcgtaccc atcaccctcc accgtatacc agaagacgtg gcccacgccg
      481 gtgccgtccg gacccagagt cggcaccacg ccgggcggga gaaggttctg cgcatagttg
      541 agccgctcca ggacccgggt acgggcccag tagatatcga cgttgtcttc gaagatgacg
      601 taaatcatcg aaaacccgaa ggccgaactg gcgcgcacta ccttcaggtg tggcagtccc
      661 tgcaggttcg aggccagagg gtaggtgatc tggtcttcga tgaccttggg gctgcggccc
      721 ggccattcgg tgaaaacgat cacttgatta tcggataagt cggggatggc gtccaggggg
      781 gtgcggaaga gcgcccagat cccccagctg acgacgagga tgaagagtaa gatgatgatg
      841 aaacggtttt tggccgaata ggcgatgatt ttctctatca tggcttagcc cttttgtgaa
      901 tctcgtctgc ggggagggtc gagttcactc gaccgttgtg accgaacgcg actgaagtcg
      961 cgtctcccaa gtcaactgac tctctctgta gccccgctct ccagaccagg gctacaggcc
     1021 ccgcaagatc aatggaatct gacgttatgc ctcgtgagaa agaacccatt ttttaattct
     1081 cctccggtta catccccggc atgcccggca ttgcttcttt ttgtttcttt cctgacgtcg
     1141 tgtcgtggcc ggcgtgcgcc ccgctcaggc gcgccaactg gctctccgaa tcgatgagga
     1201 agccggcctg ggtcaccact ttttcgccgg cggcgacgcc cgacagaact tcgcagtaat
     1261 tccctgcccg ctggccgatc tgcacgtcac ggggggtata gacgcccggt tctttttcca
     1321 cccaggccac ctgccgctgc cccgtattga gcacggcact ggccggaatg gcgagtttcc
     1381 gacccaggtt gactttgatg cgggcatcca cgtacatgcc cggtttgagc cggtcgctgg
     1441 ggttatcgaa ctctgctctg attctcaccg tgcgggtcag cgtgtccacc accggcgaca
     1501 cgaagttgat cgtgccgtaa aacttctccc ccgggtaggc cacggcggtc acttccaccg
     1561 actggccgac acggacgttg gcgaattcgt attcgtatat ctcggcccac atccagaccg
     1621 tggagaggtc ggcgatgttg tagagcggct ggcccgcggc gacgtacata tcgaggtgaa
     1681 ccagtttgtc gatcaccgtt ccggagatgg gcgagaaaat cgtcaggtgg tctctcatct
     1741 cccgcgactg ccccaggcga tcaatctgtt ccgcggtaat cccccagagg gcaagtttcc
     1801 gccgggccga ttccaaaagc gccggattgt tactatccag ggccaggagg tactcctgct
     1861 cagcacctac gatgtcggga ctgtacatcg agaggagcgg ctggccccgc gtgacccgct
     1921 cgccggtata attgacgaag agggaatcag tccgtcccgc cacgcgcgcg gagaggttgg
     1981 cgatattccc ctccacgtag tcgatcgtgc ccacggtggt gatctctttg gccagcagcc
     2041 gcaccgccac ggcctcggtg gccacgttgg ccaagacctg ctgtgtgggc gagagccgca
     2101 cttcgtgggc gaccagttct tcctgggccg tcagggtgag ttcttccgaa ctactcctct
     2161 tcactaagtc cataccgcag atggagcagt ttcccggctt gtccgaagtc accatcgggt
     2221 gcatcgggca aacgtaaatt tccttttctt ccgcttgcgc cgtgacctgc ctcaccgctt
     2281 ttctaccggc acttctggtg aggtaaaacg ttgccagtac agcggccgcc agcaccgcaa
     2341 tccctatgat gatgcgcttc atcttctcct ccatttcatt tcatcacccg tcacttttgt
     2401 agcgttactc ccgtctcggc ctccagacgg gcgatgctct tctggtactc agacaccgtc
     2461 ataaagtagt caatctggta gtccaaaagg ttcagttgat tcgaaagcca ggtcaaaacg
     2521 tcgattttcc ccacctggta gttcacttgg gccgcctgca cggcaagttg cccctgcggg
     2581 agaatcgtct tttcatagag caggataagc ctcccgttcc ggtctatttc ggtgaggtag
     2641 tcgtcaattc tgagcggcac gtcgtgcttg gcgtggtggg tcaaaagcga aaccgcgttc
     2701 gtcagctcca gactggcctg ggcggcctct tttccctgac gccggctgga gtttatgggc
     2761 aggttcaatc ccagacgggc ggtgaccata ttatccagat cctgcccggg cataccggga
     2821 gtccgcaagc cctcgcggat accgtacgcc accgaccagg tgaaatcggg ccaggcctct
     2881 cttttggcgg ctttatactc tgcctgcgct ttgaggatgt cggcgcggag catcttgagc
     2941 atcggccggg tttcagtcaa accggccaga agggagtcgc gattcggggt atagagaacc
     3001 ggctttacct cttcgggaat aatatccggc gtttccggcc cgcggtctaa aagcgatttc
     3061 agttcatttt gaaccgccac tgtcctcttc tctaagttgg ccaacttctc ctccaaccgc
     3121 gcctgctcga cggtggccat taagaggtct tgctggagtc ccgtgcccac ttcgtagcgg
     3181 gtgcgggtta aggcgacaaa atcggagagg atggtaatcg ccttttgggt caactcctgg
     3241 cttctgcgct gggcgaaaag ttcgaaatag gtttccttca cctgccggat caactcgagt
     3301 ttctgttctt cgtagttcca gcgcgccacc tccactgctt ggcgaacgac gttctttttc
     3361 gtcccgagct tcccgaagaa gggaaacatt tgtgagatgc ctatcgactt catcgtcatc
     3421 tcatcctcgc ggaaagagaa actggtcggc aggttctcca cccccagcat cagttccggg
     3481 tcggggaggt agcccacccg cgccacctcc gcctgcgcct tttcgatatt gcgggcggcg
     3541 acccgcagat tgggattcac gcggaccgct tctgcaacca gctgcgccag aggcgtccgg
     3601 gtcgtatcct gcgctgggga cacgcccggg aaaaacccta ttgccaggca actgaaaagt
     3661 aagaatgacc ctttcaaccc actccgaatt ctccgcatag ccgctccttt tcccactcct
     3721 tgctcaaaag aaatctacca tagcggccga gccagccgca atagaaacaa tcaccctctc
     3781 gtgaaaggtg acggcttctc tccacaatga aaagccgccc aatcagagtt ggatgagttt
     3841 taggaaagga gcgttttagg aggtggcgga gagggttcgc tggagcgcga gattggctga
     3901 gaattaacta ccggcgaaac gagtctatcc aggagaagtt ttgtctcggg gagcggaacg
     3961 gtggctgcta ccgtcgttcc ctggcaagag caacccaaaa gacacttgca ggg
//
LOCUS       DBNGZA010000322         5770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1756514, whole genome shotgun sequence.
ACCESSION   DBNGZA010000322 DBNGZA010000000
VERSION     DBNGZA010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1756514"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1090
                     /locus_tag="AC16FU_07025"
     CDS             140..1090
                     /locus_tag="AC16FU_07025"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR2994160.1"
                     /translation="MGKTAIFYHPDFLLHNAGQDHPERPDRLLAIVDRLKQEPFAGKL
                     EWVEPGSAFPTWITTVHAETYRAQIEALSRAGDFRLDPDTAGGPDTYRAAMRAAGAVL
                     TAVDRVMKKENPTAFCLVRPPGHHALPDRGMGFCIFNNVAIGVRYLQEKYGLKRVTII
                     DWDAHHGNGTQAIFYRDPTVFYCSLHQYPFYPGTGKTDEVGAGPGKGFTVNCTLPAGS
                     GDQEFLAAFNDIFLPRLKDFAPEFIFLSAGFDAHRADPLTGLQVTEAGFREASRLVRA
                     LAQECCQGRLVSVLEGGYNLAALARSVAVHIEALLDEEHA"
     gene            1083..2282
                     /locus_tag="AC16FU_07030"
     CDS             1083..2282
                     /locus_tag="AC16FU_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR2994161.1"
                     /translation="MPKRKNRPHPPVAVATCAVHAGEEERKYARAISFPIFQTTNFQF
                     RTMREFYRYLKGEPDQYLYSRYTNPTIEAAEKKMAALEGAEKALLFSSGMAAISGAIL
                     TLAQTGQELVATRTLYGGTFQLLTEILPRLGIRTRFVDTQDLEQAEKVIGRKTALFYT
                     ETPTNPNNQIVDLAQAVAIAHRRRIPVVLDNTFATPLNQRPLKFGVDVVVYSATKYLA
                     GHSDLIAGFIVGTKKFCTRASLYRKILGGSHDPFGAWLLLRGLKTLALRVARQNRNGQ
                     AVAEFLLQHPKIRRVFYPGLPSDPGHRLAKRQMTGFTGMVCFEVSDGLKNVEKVMDRF
                     RVILHATSLGGVESVAHIPVLTSHIQFSPAELKKADVTPGMIRLSCGIEDPGDLKADL
                     DHALRVL"
     gene            2534..3490
                     /locus_tag="AC16FU_07035"
     CDS             2534..3490
                     /locus_tag="AC16FU_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR2994162.1"
                     /translation="MPKIDRLVEKHFEKFLVLGLALGVVLLDLFVIQKTVFLNFYYRP
                     VLVSGYVLGRRSAILTAFLCIGLVAFAASVNPSFVTSSGSSTTLALDLVIWGGFLILA
                     AYVVGTLYQHKEQKIEELKNAYIGVIEILTKYLEANDRYTRGHSVRVADYATDIASLL
                     RLPKEEVQQIRAAALLHNIGRMEISSNLIRKAAKLTIEKHEIMGTETEKGTELLRAVG
                     DVLEAALPITLAFHQYYIQGSDGDKPLADIPIGAQILHVADRFDEMTVDSAYRKSISP
                     EEAIGHLEESAGARYQPEIVAALKRVYAAKGEGQPQMFAYQD"
     gene            complement(3632..4366)
                     /locus_tag="AC16FU_07040"
     CDS             complement(3632..4366)
                     /locus_tag="AC16FU_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994163.1"
                     /translation="MSDLQEPKPFEKFGMAINELLRYGFGGALFIVAFTFVGSPEVSN
                     IKKEASGVLVIFLAFALGAILHAASRLLIEFIFWPSRYLIVWGIAFVLENITGRGLWA
                     EQNSSFHLLRRQFQVRYFDVLNAFRVVRDEFFEEKRQKWFRIEHSEIHLVYVATLVSG
                     LTTIYVALNPFDSSGNFAPQNAPSVTVLGWLTAVLLFVAVTSDILLAQRECAYLRTLI
                     ELNEGVKTQIRKLLGAAGYLKDDLGA"
     gene            complement(4376..4807)
                     /gene="aroQ"
                     /locus_tag="AC16FU_07045"
     CDS             complement(4376..4807)
                     /gene="aroQ"
                     /locus_tag="AC16FU_07045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR2994164.1"
                     /translation="MKKVLVVNGPNLNLLGSREMEIYGRQSLEEINGLIQAEADKLGA
                     EVKFVQSNSEGGLIDLIQEARAWTEGIIINPGAYTHYSYAIRDCLTAVRKPTIEVHLS
                     NLHAREEFRHHSVIAPACKGQIVGLGAKGYLLALRFLVEMG"
     gene            complement(4804..>5770)
                     /gene="aroB"
                     /locus_tag="AC16FU_07050"
     CDS             complement(4804..>5770)
                     /gene="aroB"
                     /locus_tag="AC16FU_07050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR2994165.1"
                     /translation="SLMQVFSGRKKRTVFIKLPSGEKTKTLTTCQKIYTQLLRRRFDR
                     GDLVIALGGGVVGDIAGFVAATYARGVPFVQVPTTLVAQCDAAIGGKVGVNHPLGKNM
                     IGAFHQPCFVFVDPTFLKTLPGRELRQGLAEVVKYALIRDRKLFRQLETKLPRWAVGR
                     LDGFEPVLVRCCQIKAEIVARDERDYGLRHLLNFGHSIGHALEAAADYGRFLHGEAVV
                     LGILAANWIAVGKKTLSPDESNRAGKLLALLARRMPRVRFPIEKALSAVAVDKKHRQG
                     TLRFVLLRRIGQAVIREDVTTAETKKALVFLRDWLQTTGRAKGAG"
BASE COUNT         1269 a         1758 c         1510 g         1233 t
ORIGIN      
        1 tcaataccat acggtaaaat ttcccagcgg gcaagaaatt tttctgcgcg tggccggagt
       61 atccccgctc tctcgttact tgcgttaaag cgttgatgaa ctaaattgcg acgcaaccag
      121 gtgacgcaaa gcggtcctaa tgggcaagac agcgatcttt taccatcccg atttcctgct
      181 ccacaatgct ggtcaagacc atcccgaacg gccggaccgc ctgctggcga tagttgaccg
      241 cctgaagcag gagccctttg ccgggaagct ggaatgggtc gaacccggct cggcgtttcc
      301 gacctggatc accaccgtcc atgccgaaac ctaccgggcg cagatcgagg cgctttcgcg
      361 cgccggcgac ttccggctcg atcccgacac cgccggaggg ccggacacct atcgggcggc
      421 catgcgggcc gcgggtgcgg tcctgaccgc cgttgataga gttatgaaaa aagagaatcc
      481 caccgccttc tgtctcgtcc ggccccccgg gcatcatgcg ctgcccgacc ggggtatggg
      541 tttttgcatc tttaacaacg tggcgatcgg cgtacgctac ctgcaggaga aatatggctt
      601 gaaacgagtg acgataatcg attgggacgc ccatcacggc aacggcaccc aggcgatttt
      661 ctatcgggat ccgaccgtct tttactgcag cctccatcag tacccgttct atcccggcac
      721 cgggaaaacc gatgaggtcg gtgccggccc ggggaaaggg tttacggtga actgcaccct
      781 cccggccggt tccggcgacc aagaatttct ggcggctttc aatgacattt ttttaccgcg
      841 cttgaaagat tttgccccgg agttcatttt cctctccgcc gggtttgacg cgcaccgggc
      901 tgatcccctg accgggttac aggtgaccga agcggggttt cgggaagcga gccgtctcgt
      961 gcgcgctctg gcgcaggaat gttgtcaggg ccggttggtt tcggtcctgg aaggcggcta
     1021 caatctggcc gcactggccc ggtcggtggc cgtccatatc gaggcacttc tggacgaaga
     1081 gcatgcctaa acgcaaaaat cgtccgcacc cgccagtagc cgtggccacc tgcgccgtcc
     1141 acgccggtga ggaagagaga aaatatgccc gcgccatctc cttccccatt ttccagacga
     1201 ccaacttcca gttccggaca atgcgggagt tctatcgata tctgaaaggc gaaccggatc
     1261 aatatctcta cagtcggtac accaacccga ccatcgaagc cgccgaaaag aaaatggcgg
     1321 cgctggaagg cgccgaaaag gccctcctct tctcctccgg aatggccgcc atttcgggag
     1381 caattctgac cttggcccaa accggacagg aacttgtcgc cacccgcacc ctctacggcg
     1441 gcacgtttca attgttaacg gagatcctcc cgcgcttggg gatccggacc agattcgtcg
     1501 atactcaaga tcttgagcag gccgaaaaag tcataggccg caagactgcc ctattctata
     1561 ccgagacccc gaccaacccg aacaaccaga tcgtcgatct cgcgcaggcc gtggccatcg
     1621 cccaccgccg ccggataccg gtggtgctgg acaacacctt cgccacgccg ctgaaccaga
     1681 ggccgctgaa attcggcgtg gacgttgtcg tctacagcgc cacgaaatac ctggccggcc
     1741 actccgacct gattgccggt tttatcgtcg gcaccaaaaa gttctgtacc cgggcgtcgc
     1801 tctatcgcaa aatcttaggc ggttctcatg atcctttcgg cgcctggctt ttgctgcgcg
     1861 gcctgaaaac gctggcgctg cgggtggccc gacaaaatcg gaacggtcag gcggtggccg
     1921 aattcctctt gcagcacccc aagatcaggc gcgttttcta cccgggcctg ccttccgacc
     1981 ccgggcatcg gttagccaaa aggcagatga ccggcttcac cgggatggtc tgcttcgaag
     2041 tatccgacgg gctcaaaaac gtcgaaaaag tgatggatcg tttccgggtg attctccacg
     2101 ccaccagcct gggcggcgtg gaaagcgtcg cgcacattcc ggttttgacc tcgcacatcc
     2161 agttctcacc ggcggaactg aagaaagccg acgtgacgcc cgggatgatc cgcctctcct
     2221 gcggcattga ggatccgggc gacctcaagg ccgacttaga ccacgccctg cgcgttctgt
     2281 aacgctttgc tggaactgta gcgacgctct ctttagcctg agcttgccga aggcttgggg
     2341 ttcggttgtc ggccctgctc ggagaccggg gctgccctcg gcaagccggc aacggaacaa
     2401 tcccatcccc gaccccggaa aagcacccct ggcttcaata cccagccggc ggaccggaca
     2461 aaggcacccg ctaatcaaat agaactttac tccgatataa gaaatgagcg gaaaatctta
     2521 ggggatgctg cctatgccga aaatcgatag actcgttgaa aagcacttcg agaagtttct
     2581 ggttctgggg ctggccctgg gagtggtgct cctcgacctg ttcgtcatcc agaaaacggt
     2641 cttcttgaat ttctactacc gccccgttct ggtttcgggc tacgtcctgg gccgccggag
     2701 cgcgattttg accgctttcc tctgcatcgg cctggtggcc ttcgctgcct cggtcaatcc
     2761 ctcctttgtc acttccagcg gctcctcgac caccctggcg ctcgacctgg tgatctgggg
     2821 cggatttttg atcctggccg cctacgtcgt cgggacgctc taccagcaca aggagcagaa
     2881 aatcgaggag ttgaagaatg cctacatcgg ggtgattgag attttgacca agtacctcga
     2941 agccaacgac cgctacactc ggggtcactc ggtgcgggtg gccgactacg ccaccgacat
     3001 cgcctcgctc ttacgcctgc cgaaagagga agtccagcaa atccgggcgg cggcgctttt
     3061 gcacaacatc ggccggatgg aaatcagttc caacttgatt cggaaagccg ccaaactcac
     3121 catcgagaag cacgaaatca tggggactga gacggagaaa ggcaccgaac tcctacgcgc
     3181 cgtcggcgac gttttggaag cggcactccc cattaccctg gccttccacc agtactacat
     3241 tcaagggagc gacggggata aacctcttgc ggacatcccc atcggcgccc aaattttgca
     3301 cgtggccgat cgcttcgacg aaatgaccgt cgattcggcc taccgaaaat cgatctcacc
     3361 ggaagaagcc ataggccacc tggaggagtc tgccggcgcc cgttaccagc cggaaattgt
     3421 ggccgccctc aagcgggtct acgccgccaa aggcgaaggc cagccccaga tgttcgcgta
     3481 tcaggattag cgcccctcaa accagccagc cagaatcaat atgaaagtgt ggctccggtt
     3541 ttctgaccgg ggtgaatttt cgacgctcag gtgagtgggt attttcctcc catattgtag
     3601 gacctgccct tccccaagcc ggatagacta tttacgctcc cagatcatct tttaggtagc
     3661 cggctgcccc taagagcttt ctgatttgtg tttttacgcc ttcatttaat tcaatcaacg
     3721 ttcgtaagta ggcacattcg cgttgagcca aaagaatgtc cgaggtaacc gctacaaaaa
     3781 gcaacaccgc tgtaagccat cctaacactg tgactgacgg agcattctgc ggagcgaagt
     3841 ttccagagct atcgaatgga tttagtgcta catatatcgt tgtaagccca gaaactagtg
     3901 ttgctacata tacaagatga atttcgctat gctctattcg gaaccacttc tgacgtttct
     3961 cctcgaaaaa ctcgtcgcga actacccgaa aggcgttgag gacatcaaaa taccttactt
     4021 ggaattgtcg tcttaataaa tgaaaagaac tattttgctc agcccaaaga cctcttcctg
     4081 ttatattttc caaaacgaat gcgattcccc atactatcaa atatcgactc ggccaaaaaa
     4141 tgaattcaat taaaagacgt gaggctgcat gcagaatagc cccgagtgca aaagcaagga
     4201 atatgaccag gacaccactg gcttcctttt tgatattgct tacctctggg gaaccgacaa
     4261 aggtaaatgc gacaataaac aacgctccac caaagccata acgaagcaat tcatttattg
     4321 ccattccaaa tttttcgaac ggctttggtt cttgtagatc actcaaggta aaatcctacc
     4381 ccatctccac cagaaaccgc agcgccaaaa ggtacccctt ggctcccagc ccgacgatct
     4441 gccctttgca ggccggcgca atcaccgagt ggtggcggaa ttcttcccgc gcgtggaggt
     4501 tggagaggtg gacctcgatg gtcggcttgc gcaccgccgt aaggcaatcc cggatggcgt
     4561 atgagtagtg cgtgtacgcg ccggggttga tgataatccc ctcggtccac gcgcgcgcct
     4621 cctgaatgag atcgatcaag cccccttcgc tgttggactg gacaaacttc acctccgcac
     4681 ccagtttgtc agcttcggcc tggatgagcc cgttgatttc ttctaaactc tggcggccgt
     4741 agatctccat ctcccggctc cccaggagat tcagattcgg cccgttgacc accaagactt
     4801 tcttcacccg gctcccttcg ctcggcctgt agtctgcaac caatctctca aaaacacaag
     4861 tgcttttttg gtctcggcag tggtaacgtc ctcacgaatg accgcctggc caatgcgtcg
     4921 caagagcaca aagcggagcg tcccctgccg gtgtttttta tccaccgcaa cggcggaaag
     4981 ggctttttcg attggaaacc gaacccgggg cattctccgt gccaaaagcg ccaaaagctt
     5041 tccggcacga tttgattcat ccggagagag cgtctttttc cccaccgcga tccaattggc
     5101 cgccaggatt cccaggacca ccgcttcccc atgcaggaat ctgccgtagt cggcagccgc
     5161 ctcaagcgcg tggccgatgc tatggccgaa gttcaacagg tgccgcagac cgtaatcgcg
     5221 ctcgtcccgg gcgacgatct ccgctttgat ctgacagcag cgcacgagca ccggctcaaa
     5281 gccgtccagt cgtcccaccg cccaccgtgg taatttggtt tccagttgcc ggaagagttt
     5341 tctatctcga attaaagcat acttgactac ctcggccagg ccctgtcgca attcgcgccc
     5401 gggaagcgtt ttcagaaaag ttggatcgac aaagacaaag cagggctggt ggaacgctcc
     5461 gatcatattc ttccccagcg ggtggttcac gcccaccttg ccgccgatgg ccgcatcgca
     5521 ctgggccacc aaagtcgtcg gcacctgaac gaacggcaca ccgcgcgcgt aggtcgccgc
     5581 cacgaatccg gcaatatccc ccaccacccc tccgccgagg gcgatgacca gatcaccccg
     5641 gtcaaaacgt cgtcgtaata gttgcgtgta gattttctga caggttgtga gcgtcttcgt
     5701 tttttctccg gatgggagtt ttatgaatac cgtccgcttt tttcttcccg agaacacctg
     5761 catcagcgat
//
LOCUS       DBNGZA010000323         3632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2711655, whole genome shotgun sequence.
ACCESSION   DBNGZA010000323 DBNGZA010000000
VERSION     DBNGZA010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2711655"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1627
                     /locus_tag="AC16FU_07055"
     CDS             <1..1627
                     /locus_tag="AC16FU_07055"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2994166.1"
                     /translation="VMKEIVAARIDLQPLPLRQIPPRLQAIVQKALDKDLFRRYQSAG
                     EMQIDLTTYQKTAGTILPQVELARWCANYLVGHQVEEKKESVSAVLKRVSEDSEQVIA
                     TKEVTLSEQKYDEEEEVLFETQKIAEVTAPPAPVEPKKKMPVAVSQPPLREKTPVPAP
                     RSSGKKPLWPWAVAAILLLALTADFSLGLSPLGKKLYKGRTGLSTFIASVPPGATVFI
                     DGKAIDQPTPAVVSEMKTGKHSVILSFPGYADLHTEVDCRTGQANRFNFAFRGTLEIA
                     SEPAGAEVFCNGVKEPQRTPVALSVPIDSVLSVELVYGKSRLKPISFSLLTLANDVGA
                     SWQVEKVASDHLRLTGRFTRELVFRSTPPGADLFIDHESKPAGNTWSAPLVSVPFGKH
                     TVTARLAGYAPQHIMLEVSEKTTGEYSFSLADQTARRGTSSSQPFTGSKDGKSLSLAN
                     LNSKVVVTVQDEQKKPLSGAQLVALNLDSKERRPLGKTDSQGALACDLPDGRWKVSIF
                     LVSYQTESKEFRVRSGSQEKLQFVLRKLEPERP"
     gene            1695..2651
                     /locus_tag="AC16FU_07060"
     CDS             1695..2651
                     /locus_tag="AC16FU_07060"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR2994167.1"
                     /translation="MKIEIAARTDLGRVRERNEDAFLVLPEQRLAMVCDGMGGHRAGE
                     VASRGAVTTIKELANPTHRERLTGLFFSIDQKLPPAVRQLISSLRVANRRIYNAAQGD
                     VSLRGMGTTVVAALCDSGGCAIGHVGDSRAYLFRGGVIHRLTSDHSWLAELIAAGELT
                     VADEHSFAERNVITRSLGTRPVVKIDLRWDPVEKDDLILLCSDGLSGHLKDPDLLKVI
                     TAKKKKLEAVVEQLIKRANEAGGEDNITCALIRVVETDTTPVERFQGTVQEETDGELT
                     VEDQIIQSLLGGKKLQSEVSFESTDAIRIKAKEKKKKFSLFS"
     gene            2869..3126
                     /locus_tag="AC16FU_07065"
     CDS             2869..3126
                     /locus_tag="AC16FU_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2994168.1"
                     /translation="MVNGKFYVYVLKSQVDERTYTGSAANVTARVRAHNAGRVSASRL
                     HRPYDLVYTEEFQTRAEAMAREKFFKTVAGRIELRLVLQQL"
     gene            3151..3223
                     /locus_tag="AC16FU_07070"
     tRNA            3151..3223
                     /locus_tag="AC16FU_07070"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3184..3186,aa:Ala,seq:cgc)
     gene            3399..>3632
                     /gene="ffs"
                     /locus_tag="AC16FU_07075"
     ncRNA           3399..>3632
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16FU_07075"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          827 a         1015 c         1096 g          694 t
ORIGIN      
        1 cgttatgaag gagatcgtgg cggctcggat cgatctccag cccctcccct tgcgccagat
       61 tcccccccgg ctgcaagcaa ttgtgcagaa ggcgctggac aaggatctct tccgtcgcta
      121 ccagagcgcg ggagagatgc agattgatct caccacctac cagaaaaccg cagggacgat
      181 tctcccccag gtggagttgg cccggtggtg cgcgaattac ctggtgggac atcaggtcga
      241 agagaaaaaa gaaagcgtca gcgcggtcct gaagcgggtg tcggaagatt ctgagcaggt
      301 gattgccacc aaagaagtga ccctctcgga gcagaagtac gatgaggaag aagaagtatt
      361 atttgaaacg cagaaaatag ccgaagtgac tgctccgccg gcgccggttg aacccaaaaa
      421 gaaaatgcct gtcgcggtct cccagccgcc tctccgggaa aagacgccgg tcccggctcc
      481 ccgctcttcc ggaaaaaaac ccctctggcc ctgggcggta gcggccatcc tgctcttggc
      541 gctgaccgct gatttttcgt taggtctttc gcctctgggg aagaagttgt acaaaggcag
      601 aaccggcctg agcacattca ttgcctcggt accgccggga gcgacggtat tcattgacgg
      661 caaagcaatc gaccagccga cgcccgccgt ggtgtctgaa atgaagacgg ggaaacacag
      721 cgtcattctt tccttccccg gttatgccga cctccacacg gaggtggatt gccgaacggg
      781 gcaggcgaat cgcttcaact ttgctttccg cggcactctc gagatcgctt ccgaaccggc
      841 gggagcggag gtattctgta acggtgtcaa ggagccgcag cgcacacccg tcgcgctcag
      901 cgtaccgatc gattcggttt tgtctgtgga gttagtgtac ggcaagtcgc ggctcaaacc
      961 gatctccttc agcctcctga cgctggccaa cgacgtcggc gcgtcgtggc aggtcgaaaa
     1021 ggtcgcctcc gatcatctcc gcttgaccgg acggttcacc cgtgagctcg ttttccgctc
     1081 caccccgccg ggggccgatc tgttcatcga ccatgagtcc aagccggcgg ggaatacctg
     1141 gtcggcacct ttggtaagcg tgccgttcgg caaacacacg gtcacggcgc gcctggcggg
     1201 ttatgcgccg caacatatca tgctcgaggt ttcggagaaa accacgggcg agtattcttt
     1261 cagcttggca gaccaaaccg cgaggcgggg gacgagcagt tcacagccgt tcaccggcag
     1321 taaagacggg aaatcgcttt cgctggcgaa cctgaacagc aaagtagtcg taaccgtcca
     1381 ggacgaacag aaaaaaccat tgtccggcgc acagctggtg gcgctcaatc tggactccaa
     1441 agagcgacgc cccctgggga aaaccgacag ccagggcgcg ctcgcctgcg atttacccga
     1501 cggccggtgg aaagtttcga tttttttggt ttcttaccag accgagtcga aagagtttcg
     1561 cgtgcgctcc gggtcgcagg agaaactgca gttcgttctg agaaaactgg agccggagcg
     1621 gccatgagcc ggtttgatag accgggtaga aaatccttga aacgaacgaa acaatacatt
     1681 atgataggcc ggatatgaaa atcgaaattg cggcccggac cgatctggga cgggtacggg
     1741 aacggaatga ggatgctttt ctggtgttgc cggaacagcg gctggccatg gtctgcgacg
     1801 gcatgggggg acaccgggcg ggcgaagtcg ccagccgcgg ggcggtgacc accatcaaag
     1861 aactggccaa cccgacccac cgggagcggc tgaccggact tttcttttcc attgatcaga
     1921 aactccctcc ggcagtgcgt caattaatct catccctgcg ggtggcgaac cggcgcattt
     1981 ataacgccgc ccagggtgac gtgtccctgc gcgggatggg gaccacggtg gtggcggccc
     2041 tgtgcgattc gggcggctgc gccatcggcc acgtggggga cagccgcgcc tacctcttcc
     2101 gcggcggcgt cattcaccgg ttgacgagcg accactcctg gctggcggaa ctcatcgccg
     2161 cgggcgaact gaccgtggcg gacgaacact cctttgccga gcggaacgtc atcacccgct
     2221 ctctggggac gcggccggtg gtcaaaatcg acctgcgctg ggatccggtg gaaaaagacg
     2281 acttgatcct cctgtgcagc gacggcctct ccggccacct gaaggacccg gacttgttga
     2341 aggtcatcac ggcaaaaaag aaaaaattag aggccgtcgt cgaacaactg attaaaagag
     2401 cgaacgaagc ggggggcgaa gacaatatca cctgcgccct catccgggtg gtcgagaccg
     2461 acaccacgcc ggtggagcgc ttccagggaa ccgttcagga agaaaccgac ggcgaactga
     2521 ccgtggaaga ccagatcatc caatcgctcc tggggggcaa gaagttgcag tcggaagtgt
     2581 ccttcgaatc caccgacgcc atccggataa aagcaaaaga gaagaaaaag aaattcagtt
     2641 tgttctctta accgagtcat tccatccgcg tcctgattct cccgtaagcg ctcagtattt
     2701 tttctcctgc cactcatcct ggaaccagcg tgatcggctc gttgatttcc cacttgagaa
     2761 actgttatat tcggaaaaac ggaaggaacg gaatctctct ggggcagtag ctcagtccgc
     2821 ctgaggcgga ttcgaagcgc tccgctccat agatagtcga gaagcagaat ggtcaatgga
     2881 aagttctacg tttatgtttt aaagagtcag gttgacgaac ggacatacac tggttctgcc
     2941 gctaatgtta cggctcgtgt ccgagcgcac aacgctggac gagtctcggc ctcgcggcta
     3001 caccgaccgt atgatttggt gtatactgag gaattccaga ctcgtgcaga ggccatggct
     3061 cgcgaaaaat tcttcaaaac cgtagcagga cgaatcgagt taagattggt tttacagcaa
     3121 ttatgagggt aaaaaggaat aaggaaatct ggggcagtag ctcagctggg agagcatcac
     3181 gttcgcaacg tgagggtcgg cggttcgaat ccgctctgct ccataataac tttggtgaag
     3241 gaggaaattg aaaggatgga gcgcagcgat ttcggtcggt acgactttcg gtcgttccgc
     3301 ccctaacaac atgagggtcg gttgagcgga gcgaaagtcg tatcgatccg cctgaggcgg
     3361 attcgaatcc gctctgctcc atggaaaaaa agatgcgctc gtgctagatg gggagttagc
     3421 ggtgccctgt aacccggaat ccgctctacc gggatcgacg cccaccggag gtctcactac
     3481 cgcggatcgt gcggccaggg gccgacgttg acggtcaggt cctgcgcaac acggaggcgc
     3541 ccaaccccgc caggcccgga agggagcaac ggtaagcgtt tttccgggtg tgccgcaggg
     3601 tcgccgggcc cgagccggtc accggatacg ca
//
LOCUS       DBNGZA010000324         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_806720, whole genome shotgun sequence.
ACCESSION   DBNGZA010000324 DBNGZA010000000
VERSION     DBNGZA010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_806720"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="AC16FU_07080"
     CDS             <1..1350
                     /locus_tag="AC16FU_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR2994169.1"
                     /translation="LRDSTLPNLGIQFRIARGQEVHFGRVTVEGLQYTRPHVATRELV
                     FHPGDRFSREKIVSSQQRIYSTGLFTFVNLVPQSSGAGGDSASRAQPARPDFILKLVE
                     RKPAFFGFKAGAGQYQQQEQQQDLAVDLGTQWGNRNLWGTARKYNIELKTSFLVINRF
                     RILNNRIAGAYQEPWFLGTRTAANFTLSFEPGVQSAVLPYRIETITGGLSLVRELSLT
                     TRLLLTGLFQHVAIFGITPENILAFKRDQGITIRRRVILSFERDTRDNIFIPLSGAQT
                     YLSGELTGGPFGGDAEFFKLVANWARYRSLSGSNVFAVRVKAGYLEGLRAGSFIPASD
                     RFFLGGGSSLRGYTENSLGPLSASGDPLGGRTLLLANGELRRPLFWKFWGSMFADVGN
                     IWERPVDFSLGDLRITTGAGLQFLTVVGPIRIDYGRRVVRGAAQPGGRVHFSILYAF"
     gene            1369..2109
                     /locus_tag="AC16FU_07085"
     CDS             1369..2109
                     /locus_tag="AC16FU_07085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR2994170.1"
                     /translation="MSYPLIGITCGAVPEAAGKRKPTRLAYDFLKRQYYEAVEAAGGT
                     PVLLPNSQNPETVAALVERLDGILFSGGEDVDPSFYGEQTKAKNLQLAAQRDQFELQL
                     IKAVPEQRLPVLGICRGHQVLNVGRGGSLFQDLALRSEPTLNHAQGGSGTYLVRHGVE
                     VKKGTRLFAIVKSEHIDVNTSHHQMVKTVAPGLLVNALSRADGVIEGLEDPEHPFWIS
                     VEWHPEAMPKDESSVRLFGAFIESARKR"
     gene            2106..2750
                     /gene="thiE"
                     /locus_tag="AC16FU_07090"
     CDS             2106..2750
                     /gene="thiE"
                     /locus_tag="AC16FU_07090"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR2994171.1"
                     /translation="MTAKEKKLRSVRLCLVTDPHLTLGRPIESIVEQALRAGVQMVQL
                     RDKETPSGLLVDLGMKLKKLCRRYRALFIVNDRIDLAKYLEAEGAHLGQEDLPLGFAR
                     KILGRGAIIGGSANSVASAKKAQQAGADYLGVGTIFPTETKVIEKVTGVELLREVRKA
                     VKLPLLAIGGVKPENCRVCIEAGADGVAVVSGIMSAPDVSAAAKAYLAEIARVH"
     gene            2840..>3062
                     /locus_tag="AC16FU_07095"
     CDS             2840..>3062
                     /locus_tag="AC16FU_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994172.1"
                     /translation="MWAVRLLLLLVIVMVIIGFSIYNSAEKVQVNLVTARYVDVPMIF
                     VAYWAFVIGMFVSFVLGVSYYLRATNQLRD"
BASE COUNT          624 a          855 c          942 g          641 t
ORIGIN      
        1 ctgcgcgaca gcaccttgcc gaatctgggg atacaattcc ggattgcccg ggggcaggaa
       61 gtccacttcg gaagggtgac ggtggaaggg ctccagtaca cccggccgca cgtggccacg
      121 cgggaactgg ttttccatcc gggcgaccgc ttcagccggg aaaaaattgt ctcttcccag
      181 cagcggattt actccaccgg ccttttcacc tttgtcaatt tggtgcccca gtcttccgga
      241 gccgggggcg attctgcctc ccgcgcgcag ccggcccggc ccgatttcat cttgaaactg
      301 gtggaacgga aaccggcttt cttcgggttc aaagccggag ccggccagta ccagcagcag
      361 gagcagcaac aggacctggc ggttgacctg gggacccagt ggggcaaccg caacctctgg
      421 gggacggcgc gtaaatacaa catagaactt aagaccagtt ttctcgtcat caaccgcttc
      481 cggatattaa acaatcggat tgccggggcc taccaggagc cgtggttttt gggcacgcgg
      541 accgccgcca actttaccct ctcgttcgag ccgggggtgc agagcgcggt cctgccctac
      601 cggatcgaaa cgatcaccgg cggcctttcc ctggtgcgcg aattgtcgct gaccacccgt
      661 ttgctcttga ccggcctgtt tcagcacgtg gccattttcg ggattacgcc ggagaacatc
      721 ctggccttca aacgcgatca gggaatcacc atccggcgcc gggtgattct ctcttttgag
      781 cgggataccc gtgacaacat attcatcccg ctctccgggg cgcagaccta cctttcaggg
      841 gaactcaccg gcggaccgtt tggcggggat gcggagttct tcaagcttgt ggccaactgg
      901 gcacgctacc gatcactttc cggcagcaac gttttcgctg tgcgggtcaa agccggctac
      961 ctcgaagggc tgcgggcagg aagtttcatc ccggcgagcg accgattttt tttgggcggc
     1021 gggtcgagcc tgcgggggta tacagagaac agtctaggcc ccttgtcggc cagtggagac
     1081 cctttgggtg gacggaccct ccttttggcc aacggcgaac tgcgtcggcc gttgttctgg
     1141 aagttctggg ggagtatgtt tgccgatgtc gggaatatct gggaaaggcc cgtcgatttc
     1201 tccctgggcg atctgcgcat caccaccggc gcggggttgc agtttttgac tgtcgtgggg
     1261 ccgatccgca tcgactacgg ccgccgggtg gtgcgcggcg cggcgcagcc gggcggccgg
     1321 gtgcacttca gcattctgta cgctttctga acggagcaga aaagaccaat gtcctatcct
     1381 ttgatcggca tcacctgtgg agcagtcccg gaagcggccg ggaagcgtaa gccgacccgt
     1441 ctggcttacg acttcctcaa acggcagtac tacgaagcgg tggaagccgc cggcggcaca
     1501 ccggttcttt tgcccaacag tcagaatcca gaaacggtcg cggcgctggt cgaacgtttg
     1561 gacggcatcc tcttcagcgg tggggaagat gtcgatcctt ctttttacgg cgaacagacg
     1621 aaagcaaaaa atctccaact ggcagcccaa cgagaccaat ttgaactgca attgatcaag
     1681 gcagtccctg agcaacggct accggtgctc ggcatttgcc gcgggcatca ggtgctcaat
     1741 gtcggccggg gcggctcgct tttccaggat ttagccctcc gctccgagcc gacgctcaac
     1801 cacgctcagg gaggcagtgg aacctacttg gtgaggcacg gcgttgaagt gaagaaaggg
     1861 acacgcttgt ttgccattgt gaagtccgag catatagacg tcaacaccag ccaccaccag
     1921 atggtgaaaa ccgtagcgcc ggggcttctg gtcaatgcac tcagtcgtgc ggacggagtc
     1981 atagaaggtc tggaagaccc tgaacatcca ttctggattt cagtggaatg gcacccggaa
     2041 gcgatgccga aggacgaaag ctcggtgcga ctttttggcg cgttcatcga atcggcaaga
     2101 aaaagatgac tgcgaaagag aaaaaactcc gcagcgtccg gctctgcctg gtgaccgatc
     2161 cccatctgac tctgggccgg cccatcgaat ctatcgtcga gcaggcgttg cgggctggcg
     2221 tccagatggt gcagttgcgc gataaagaaa ctccgtccgg cctgctcgtt gatttaggga
     2281 tgaaattgaa aaaactctgc cggcgctacc gggcgctctt catcgtcaat gaccggattg
     2341 atctggccaa atacctggaa gccgaaggcg cgcatctggg acaggaggat ttgccgcttg
     2401 gcttcgcccg gaagatactg ggtcggggag cgattatcgg tggctcggcc aattcggtcg
     2461 cgagcgccaa aaaagcgcaa caggccggtg ctgattacct gggtgtaggg acgatttttc
     2521 cgacggaaac gaaagtgatc gaaaaagtca cgggcgtcga attgctccgg gaagtccgca
     2581 aggcggtgaa actcccgctc ttggccattg gcggagtaaa accggaaaac tgccgagtct
     2641 gcatagaggc cggtgcagac ggcgtcgccg tcgtctcggg cataatgagc gcgccggatg
     2701 tttccgccgc ggctaaagcg tacttggcgg aaattgcccg tgtgcattaa cgaagatgct
     2761 aaacttgatt gacacagctt tgccagaagc gatatttctc caaaaggatt gagatgtcca
     2821 tgcgaccgaa ggaggggata tgtgggcggt gcggctgctt ttgcttctgg tcatcgtgat
     2881 ggtgatcatc gggttttcga tctacaactc cgcggaaaag gtgcaggtca acctcgttac
     2941 cgcccgctac gtggacgtgc cgatgatctt cgtggcctac tgggctttcg tgatcgggat
     3001 gttcgtctcg tttgttctgg gagtcagcta ctacctgcgc gccaccaacc agttgcgtga
     3061 tg
//
LOCUS       DBNGZA010000325         6393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_909910, whole genome shotgun sequence.
ACCESSION   DBNGZA010000325 DBNGZA010000000
VERSION     DBNGZA010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_909910"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="AC16FU_07100"
     CDS             <1..1201
                     /locus_tag="AC16FU_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR2994173.1"
                     /translation="LLFHKDGAAADDRLSWSVAGAGDVNGDGKADFIIGAWAADPGGL
                     PYAGSAFVYSGADGSLLFQKNGVAAGDLLGASVARAGDVNGDGKADFIVGAPFADPGG
                     LSMAGSAFIYSGTDGSLLFQKDGSATGDAFGISTAGAGDINGDGKSDFIVGANFADPG
                     GVGYAGSVFVYSGADGSLLFQKNGTAFFDNLGNSVAGAGDVNGDGKADFIVGAYRADG
                     GGLVDAGSAFVYSGADGSLLFQINGVTANDNLGFSVAGVGDMNGNGRADVIVGARLAD
                     PGGLTDAGSAYVYSFSPTGVTKEEFAGIPSSFLLAQNFPNPFNAGTQIVFELPGAESR
                     SVRLEVFDILGQRIATLIDQSLSGGRYHVTWDGKDGQGKAVGSGVYFYRLTAGEFKET
                     RRMTLIK"
     gene            1485..2648
                     /locus_tag="AC16FU_07105"
     CDS             1485..2648
                     /locus_tag="AC16FU_07105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR2994174.1"
                     /translation="MIKEKIKQAIAILNEKKIDLWLTFVRETGTFADPAMDLVGGAEV
                     VWQSAFLISASGESIAIMGTYDTENFKRQGNYQKIIGYDQDITPVLLTEIKRLDPVTI
                     AVNYSPDNDLADGLTHGMYLLLDRIFAGTPHRNRLISAEPVLSALRGRKLPVEVTRIK
                     ESIRTSDEIFKQLLGFLKPGLTEKQVDEFVTSQFEAHKVKRAFPTIINHGAKSPVGHV
                     GPSDAVLEKGDLFHIDFGVKKDGYCSDIQRMVYFLAPGEKKPPTNVQKAFDAVRKTIT
                     TAAEHLKPGAIGYKIDAVGRQTVTACGYPEYNHALGHQLGRAVHDGGTLLGPRWPRYG
                     QATEGEVEAGNVFTLELGANVPGKGTVSLEEDVLVTERGCEFLTKPQAELICL"
     gene            2929..5067
                     /locus_tag="AC16FU_07110"
     CDS             2929..5067
                     /locus_tag="AC16FU_07110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR2994175.1"
                     /translation="MPQVTHSPAHSVDSAGELFSAKGILAQKTKDFEFRPEQQKMAEA
                     VAEALDSGKILVVEAGTGTGKTWAYLVPVLLWAQENKKRVVISTYTKNLQHQLLSHDL
                     PVIEKALGIDVPVELFKGKENYLCLLRLNRHADETTLFKEKGKFSGVFSWAEKTKTGD
                     LSEYGTNGEPLSTNLRNEVNVENHFCLHQECLYYSPCFYFKARRRAQEAAVVIVNHFL
                     FFADLVTDHSLLGHYDAVVFDEAHRVEEVASRYFGAESDYWTIRRTCERLYNERPNPS
                     GLLTIISTRINAVLHAKADKNWWQKNLSLAQEKVQGVAQAAFDFFTEAAREVPRENAD
                     DSFPIKRRLKSDDDFLRALRHPAEGLGSTLSGLAQQLGKLSNSMQEIDPEVLPEVKEY
                     AFELDTRAKELTLTRNTLLELLSAEEDDQVFWVELLPKNPLSVAFHRVPLDIKEELKA
                     KIYDQVEAAVFSSATLAVNGDFGYTCERLGLAALKSGRVNCKQFGSSFPLADQMKVFV
                     ADFVGSPLEGEYLNAISNLLSLSCRKVPAKTLALFTSHSHLERVHRSLRAELDEAGVD
                     LFGQVIDGTAEEVLERFRKARRALLLGVAALWEGIDLPGRQLELIYICRLPFAVPTDP
                     VAQARMEKLEQEGLDPFTNYSLPEAVLRFKQGLGRLIRTRNDRGAVVVLDNRILRKGY
                     GRFFAQALPVELEAVKTEGELFERLAGWLR"
     gene            5080..5964
                     /locus_tag="AC16FU_07115"
     CDS             5080..5964
                     /locus_tag="AC16FU_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR2994176.1"
                     /translation="MKKLIGAAVLVAILITVIVVVKGRQNAIDATRIEASGTIETTEI
                     DVASKTSGRVAVLNITEGDPVKKGDLLLELEHNELDAQHKVAVATIAAAKSRVSQAEV
                     NLANLKDNFARIEALLQSGAATQQVYDDMKAKVTLAEREQEAARASLAQAQAQKELIE
                     TQLENSRIISPATGYVLSKNVEPGEVILPGAPVATIGELTSVWLKIYVKETQLGHIKL
                     GQPVKVRIDSFPKRDFEGKITYISNEAEFTPKSIQTKDERVKLVYAVKVTIPNPNLEL
                     KPGMPADAVIEVGSSDDK"
     gene            5971..>6393
                     /locus_tag="AC16FU_07120"
     CDS             5971..>6393
                     /locus_tag="AC16FU_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2994177.1"
                     /translation="MGYYFGEMTRLCRFLKRCHLREVFLVLIFPFSSASGQMLYQKSG
                     AAVNDLFGYSVAGAGDVDGDRRADFIVGALYASPGGLYAAGSAYVYSGATGDLLYQLN
                     GSSSGAWFGRSMGNAGDVDGDGKDDFIVGATGSASIQAG"
BASE COUNT         1415 a         1665 c         1902 g         1411 t
ORIGIN      
        1 actccttttc cacaaagacg gcgccgccgc cgatgaccgt ctgagctggt ccgtggccgg
       61 ggccggagac gtgaacgggg acggaaaggc tgattttatc atcggcgctt gggctgccga
      121 cccgggcgga cttccgtatg ctggctcggc ttttgtctat tcgggagccg acggctccct
      181 tcttttccag aaaaacggag tagctgcggg cgatctgctg ggtgcctccg tcgccagggc
      241 tggtgatgtg aacggggatg gaaaagccga ttttatcgtc ggtgctcctt ttgccgaccc
      301 gggcgggctt tccatggccg gttcggcctt tatctattct ggaactgacg gctcccttct
      361 tttccaaaaa gatggctccg cgaccggtga tgcattcggt atctcgactg ctggagccgg
      421 ggatataaat ggagatggaa aatccgattt cattgttggc gctaatttcg ccgacccggg
      481 cggagttggg tatgcgggct cggtttttgt ctattctgga gccgatggtt ctctcctgtt
      541 ccagaaaaac ggcaccgcct tctttgacaa tttgggtaat tccgttgctg gagccgggga
      601 tgttaacgga gatggaaaag ctgattttat tgttggtgca tatcgtgccg acgggggtgg
      661 acttgtcgat gctggctcgg cttttgtcta ttccggtgcc gacggctcct tgctttttca
      721 gataaacggc gttactgcta acgacaactt aggtttttcg gtggccgggg tcggggatat
      781 gaatgggaat gggagagctg atgtaatcgt cggcgctcgt cttgctgacc cgggcgggtt
      841 aaccgacgcc ggttcggctt atgtttattc tttttccccc accggcgtga ccaaagagga
      901 gtttgccgga attccctcaa gtttcttgct cgcgcagaat ttccccaatc cgttcaatgc
      961 gggcacccag attgtctttg aattgcccgg agcggaatcg cgctcggtac ggctggaggt
     1021 atttgacatt ctggggcaga gaattgcgac gctcattgat caatctctct ctggcgggag
     1081 gtaccacgtt acctgggacg ggaaagacgg gcaggggaag gcggtgggaa gcggggttta
     1141 cttttaccgc ttgacggcgg gggaattcaa ggaaacccgg cggatgacgt taatcaaata
     1201 atttcacagc agatgaagca gatggagcgg attaattttg taggggcgaa tctttacttg
     1261 tcctgagcga agccgaagga gattcgccca agaataagcg ggtgaatccg ccttaggcgg
     1321 attcactcct acattttttg agaatttttt ggcggaggtg aacctccgca ttatatttcc
     1381 gtaaaatgat ttgacccctc cctgccctcc tcggtacgag tctatcgacc ctccgagggg
     1441 agggaaaaaa agaggggtta aggaaacctc tcagaaaggg caaaatgatc aaggagaaga
     1501 tcaaacaagc gatagcgatc ctcaacgaaa agaaaattga tctctggctc accttcgtgc
     1561 gggaaacggg cacgttcgcc gatccggcga tggatttagt cggcggggcg gaggtggtct
     1621 ggcagtcggc gtttctcatt tccgcctcgg gcgagagcat cgccattatg gggacctacg
     1681 acaccgaaaa tttcaagcgc cagggaaatt accagaaaat catcggctac gatcaggaca
     1741 ttacaccggt gcttctaact gaaatcaagc ggctggatcc ggtcaccatc gcggtgaact
     1801 actcgcccga caacgacctc gccgacggcc tgacgcacgg gatgtacctg ttgctcgacc
     1861 ggattttcgc cggaacgccg caccggaacc gactgatttc cgcggagccg gtgctttccg
     1921 ctctgcgcgg ccggaagttg cctgtggaag taacgcggat taaggaatca atccgcacgt
     1981 cggacgaaat tttcaagcaa cttttgggtt tcctcaagcc ggggctgacg gagaagcagg
     2041 tggatgagtt cgtcaccagc cagttcgaag cgcacaaggt caagcgcgcc tttcccacca
     2101 tcatcaatca cggggcgaag tcgccggtgg ggcacgtggg gccgtcggat gccgtcctgg
     2161 aaaagggcga cctgttccac atcgatttcg gcgtcaaaaa agacggctac tgctccgaca
     2221 tccagcggat ggtctacttc ctcgcgccgg gggagaagaa gccgccgaca aacgtgcaga
     2281 aggcgtttga tgccgtccgc aagacgatca ccaccgcggc ggaacacctc aaaccggggg
     2341 caatcggtta caagatcgat gcggtcgggc ggcagacggt caccgcgtgc ggctatcccg
     2401 aatacaatca cgcgctggga caccagttgg gccgggcggt ccacgacggc ggaacgcttt
     2461 tggggccgcg ctggccgcgc tacgggcaag cgacggaggg agaagtggag gcgggaaacg
     2521 tcttcacatt ggagttggga gcgaacgttc ccggcaaggg gacagtttct ctcgaagaag
     2581 acgttttagt gacggagcgg gggtgcgagt ttttgaccaa gccacaggca gagttgattt
     2641 gtttgtaagt ttttgacccc tccccagccc tcccctctaa ggggagggaa gatagatctt
     2701 cttaccctct gggcataggc gggtcggaac gactcgtacc gagactctca atcccgccga
     2761 atttcctact tcccgcgtag ggcgacccct acggaccgag ttgcaaccgg gttataacca
     2821 ggccgccccg gtcgcccctc cattttaggg attgggctgt tttaaattcg tttgaacgta
     2881 aaagcgtgat acaccatttc tgactatatt ctccccgtga atcccgcggt gccacaggtc
     2941 actcactcgc cggctcactc cgttgacagc gccggcgaac ttttcagcgc taagggaatt
     3001 ctcgcccaga aaaccaaaga tttcgaattt cgtcccgaac agcagaagat ggccgaagcg
     3061 gtggcggagg cgctcgactc cggaaaaatt ttagtcgtcg aagccggaac cggcacgggc
     3121 aaaacctggg catacttggt tccggttctg ctctgggcgc aggagaacaa aaagcgcgtc
     3181 gtcatctcca cctacaccaa aaacctccag catcagttac tctcccacga tctgccggtg
     3241 atcgaaaagg cgctgggaat cgatgtgccg gtggagcttt tcaagggcaa ggaaaactat
     3301 ctttgcctgc tgcggctgaa tcggcatgcg gacgaaacca cgctcttcaa ggaaaagggc
     3361 aaattctccg gagttttctc gtgggcggaa aagaccaaga ccggcgatct ctcggagtac
     3421 ggtactaacg gcgagccgct ttcgaccaat ctccgcaatg aggtgaatgt cgaaaaccac
     3481 ttttgcctgc accaggagtg tctgtactac tcgccctgct tctacttcaa ggcccggcgc
     3541 cgggcgcaag aggcggcggt cgtcatcgtc aaccacttcc tcttctttgc cgatctggtg
     3601 accgaccact cgcttttggg ccactacgat gcggtggttt ttgacgaagc ccaccgggtg
     3661 gaagaagtgg ccagccgcta cttcggcgcg gagagcgact actggaccat ccgccgcact
     3721 tgcgagcgtt tatataatga gcggcccaat ccttcgggtc tcttgacgat tatctccacg
     3781 cgcatcaatg ccgtcctcca cgccaaggca gacaaaaact ggtggcagaa aaatctctca
     3841 ttggcacagg agaaagtaca aggggtcgct caggcagcgt tcgatttctt taccgaggcg
     3901 gctcgtgaag tgccgagaga gaatgccgat gattcctttc cgatcaagag acgcttgaag
     3961 agtgatgatg attttttgcg ggcgctcagg cacccggcgg aaggtctcgg ctccacactt
     4021 tccggtctgg cccagcaatt ggggaagtta tcaaattcga tgcaggagat tgaccccgag
     4081 gttttgcccg aagtcaagga gtatgccttt gaattagaca cacgcgccaa agaactaacc
     4141 ttgacgcgca acactctttt ggaactcctc tccgcagagg aagacgatca ggtcttctgg
     4201 gtggaacttt tgcccaaaaa tccgctctcg gttgcgtttc accgggtgcc gctggacatc
     4261 aaagaggaat tgaaagcgaa aatctatgac caggtcgaag cggcggtgtt ctcgtccgcg
     4321 actctggcgg tcaatggcga tttcggctac acctgcgagc ggttagggct tgcggcgctc
     4381 aagagcggaa gggtaaactg caaacagttc ggctcctctt ttccactcgc tgatcaaatg
     4441 aaagttttcg tcgccgattt cgtcgggtca ccgctcgaag gagagtatct caacgcaatt
     4501 tcaaatctgc tctcattgtc ctgccggaaa gtgccagcca agacgctggc gctttttaca
     4561 tcacacagcc atttagagcg ggtgcaccga agcctgcgag ccgaactcga cgaagcaggt
     4621 gttgatctgt tcgggcaggt catcgacggc acagcggagg aagtcttaga gcgttttcgg
     4681 aaagcgcgtc gcgcgttgct cctgggcgtg gcggcgttgt gggaggggat tgacctgccg
     4741 gggcggcaat tagaactcat ctacatctgc cgcctgccgt tcgccgtgcc gaccgatccg
     4801 gtcgctcagg ccagaatgga aaagttagaa caggagggac ttgatccgtt taccaactac
     4861 tctctgcccg aggccgtctt acggttcaag caaggcttgg gccggttgat tcgaacacgc
     4921 aatgaccgtg gtgcggtcgt ggtgctggat aatcgtatat tgcggaaagg gtatggccgg
     4981 ttttttgcgc aagcgttgcc ggtggaattg gaagcggtca aaactgaggg agaacttttt
     5041 gaacggctcg cgggctggct caggtaggag agaagatagg tgaaaaagct cataggtgcg
     5101 gcggtgttgg ttgccatcct gatcaccgtc atcgtcgtgg tcaagggccg gcaaaatgcc
     5161 atcgatgcca cccgcattga ggcctccggc accattgaga ccaccgaaat cgacgtggcc
     5221 tccaaaacgt ccgggcgcgt ggccgtcctt aacatcaccg aaggtgatcc ggtgaaaaaa
     5281 ggcgacctgc tcttggaatt agagcacaat gaattggatg cccagcacaa agtggcggta
     5341 gcgacgattg cggcggcgaa gtcgcgcgtg agccaggcgg aggtgaatct ggcgaacctc
     5401 aaggacaact ttgcgcggat tgaggcgctc ctgcaatctg gtgcggcgac ccagcaggtt
     5461 tacgatgata tgaaagcgaa ggtaacctta gccgagcgcg aacaggaagc cgcgcgcgct
     5521 tctctcgctc aggcgcaagc gcagaaagag ttgatcgaaa ctcaattaga gaacagccgg
     5581 ataatctccc ccgccaccgg ctacgtcctc tccaagaacg tcgaaccggg cgaggtgatc
     5641 ctccccggcg cccctgtggc gaccatcggt gaactcacct cggtatggct gaaaatttac
     5701 gtgaaggaaa cgcagttagg ccacatcaaa ttgggccagc cggtgaaagt gcggattgat
     5761 tccttcccca aacgggactt tgagggcaaa atcacctata tttccaacga agctgagttt
     5821 acgcccaaga gcattcagac caaagacgag cgggtgaaat tggtctacgc ggtgaaagtg
     5881 acgattccca atcccaacct ggaactcaag cccggcatgc cggcagatgc ggtgattgag
     5941 gtggggagtt cggacgataa gtgatatgga atgggctact actttggcga gatgacccgc
     6001 ctttgccgtt tccttaagag gtgtcattta agggaagttt tcctggttct aatctttcca
     6061 ttttcatccg cttctggaca aatgctctac cagaaaagcg gtgccgccgt taatgattta
     6121 ttcggttatt cggtagctgg agccggtgat gtggatggtg accggagagc cgatttcatc
     6181 gtaggggctc tttatgcaag tccgggtggt ctttatgccg cagggtcagc atatgtctac
     6241 tccggtgcca ccggggatct actttaccaa ctaaatgggt cttcgtccgg ggcttggttc
     6301 ggacgttcaa tgggaaatgc cggggatgtt gacggagacg gaaaggacga ctttatcgta
     6361 ggggctacag gtagtgcttc gatacaggcc ggg
//
LOCUS       DBNGZA010000326         2316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_592593, whole genome shotgun sequence.
ACCESSION   DBNGZA010000326 DBNGZA010000000
VERSION     DBNGZA010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_592593"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16FU_07125"
     CDS             <1..509
                     /locus_tag="AC16FU_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994178.1"
                     /translation="KAPLENVQAASAAVISDSDDIANARLVFANGCVANLTASRISPK
                     PMRKVRIFQKNAYLSLDLADKKVDLYELVTNADGSLPPQAGVAGGSTVPLFQFPVEEG
                     GRAITFRQPPVPEYDMLTRELESFVRAIRTRSAPAVSAQEGRRALEVALTVLQKGQAQ
                     RRRTGIPV"
     gene            552..1685
                     /gene="lpxB"
                     /locus_tag="AC16FU_07130"
                     /pseudo
     CDS             552..1685
                     /gene="lpxB"
                     /locus_tag="AC16FU_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014714.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
     gene            1692..>2316
                     /locus_tag="AC16FU_07135"
     CDS             1692..>2316
                     /locus_tag="AC16FU_07135"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR2994179.1"
                     /translation="MGTSAIFFLYNFMWTLGLIFIFPYVVLRSLFDRTGVLERVGRYG
                     WPPITTEARRPLIWLHAASVGEVKALGQFTLLLQRHFPQAQFCLSTFTRQGKIEARKI
                     LPNVSTFYLPLDHPWPVRRAVAHLKPALLILAETELWPNLIIKAHQAGAHVFLVNGRI
                     SASSFPRYRRFRRLFAPALTAMERCWVQSDIDRARLVSLGAPAERVEV"
BASE COUNT          429 a          692 c          707 g          488 t
ORIGIN      
        1 tcaaagcgcc tctcgagaac gttcaggccg cttctgccgc ggtaatctcc gactccgatg
       61 acatcgccaa cgcccggctg gtctttgcca acggctgcgt ggccaacctc accgccagcc
      121 ggatttcgcc caagccgatg cgcaaggtgc gcattttcca aaagaacgcc tatctctcgc
      181 tcgatttagc cgataaaaaa gtggacttgt acgaactggt gacgaacgcc gacggttccc
      241 tcccgccgca agcgggagtc gccggcggtt caactgttcc cctatttcag tttcccgtgg
      301 aggaaggggg gcgcgcgatc accttccggc agccgccggt gccggaatac gacatgctca
      361 cgcgggaact ggagagtttc gttcgtgcga tccgcacgcg cagcgccccg gccgtttcgg
      421 cccaggaggg ccggcgagcc ctggaagtcg ccctcacggt gctgcaaaaa ggtcaggcgc
      481 aacgccggcg gacgggcatt ccggtctagc gctggagaaa gggaaaaagg acggcacaaa
      541 aaattttgat tgtggccggg gaagcatccg gtgatctgca cggggcgctt ttagtgcggg
      601 aactccagaa gaggcgtccc gattttttct ttttcggcct ggggggcgac cagatgcacg
      661 agcagggggt gcgtctcgcc taccacgtgc gcgcattgtc ggtcatgggt ttcttcgaag
      721 tagtgcggcg gatcggattt ttccgaaaag tgtttcgaga ggttctccgc gcggtcgagt
      781 agcaaaaacc gacggcggct gttttgatcg actaccccgg gttcaacctg cggctggcca
      841 aagcgctcaa agcgcggggg ataccggtca tctactacat cgccccgcag atcttggcct
      901 ggcggccgca aaggatcaag caaatcaaaa ggttcgttga cctgatgctc gtgattctgc
      961 cgtttgagga agagctctac cgcaccaacg gcgtgtcggt ccgctttgtg ggccacccat
     1021 tggtggacca ggtgcgcccg tccggcaccc gcgagggatt tcggcaaaaa tggaatttag
     1081 ctcctgatcg tccgcttttg ggtcttttgc ccggttcgcg tcctcaggag gtggcgcacc
     1141 acctgccggt gatgctcaaa gccgcagcac tcctcgcacg ccagggggtt acctgtcagc
     1201 cggtgatcgg gttagcaccg accatcaacc ggctaatggt tgacgaaatt ttgagatctg
     1261 ccgggcgcga tccaaaatcg aactcgactt atcgtgtggt agagcaggct acttacgatt
     1321 tgatgtgcta cgcgaaactc ttggtcgttt catccggtac ggcgacggtg gaagcggcct
     1381 tagccggcac accaatggtc gtgctttaca agacttcgcc tttcacctat cttttagcac
     1441 ggaggatggt gcgaattccc cgcattgcga tggtcaacgt tctggccagg gaggagctgg
     1501 tgcccgagtt tatccaaaaa caggccacac cggaagcagt cggccaggcg atggtccaac
     1561 tctggcagga tgaagtccga taccggcgca tccaggagcg gctggcggag gtgaaggcat
     1621 cgctcgggaa tcccggagcg gccggccgtg cggcggaggc tattttaagc cacctttccc
     1681 tctgaacgcc catgggtacc tcggccattt tctttcttta caacttcatg tggacgctcg
     1741 gcctcatatt catctttcct tacgtcgtcc tgcggtcact ttttgaccgg acgggggtgc
     1801 tggaacgagt cggtcggtac ggctggccgc caatcactac ggaagccagg cggccgctca
     1861 tctggctgca cgccgcgtcg gtgggtgagg tgaaagccct ggggcagttc accctgctcc
     1921 tgcagcggca ttttccgcag gcgcaattct gcctttcgac ctttacccgc caggggaaaa
     1981 tcgaggcgag aaagattttg ccgaacgtct ccacattcta tttgcctttg gaccacccct
     2041 ggccggtgcg ccgtgcggtc gcccacctga aaccggcctt gctcatcctg gcggaaacgg
     2101 aactctggcc caatctgatc ataaaagcgc accaggccgg agcgcacgtc tttttggtca
     2161 acggccgcat ctcggcctcc tctttccccc gttaccggcg tttccggcgg ctctttgccc
     2221 cggccttgac ggccatggag cggtgctggg tccagagcga tatcgaccgg gcgcggctcg
     2281 tctctctggg cgcaccggca gagcgggtgg aggtgg
//
LOCUS       DBNGZA010000327         4583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_166434, whole genome shotgun sequence.
ACCESSION   DBNGZA010000327 DBNGZA010000000
VERSION     DBNGZA010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_166434"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="AC16FU_07140"
     CDS             complement(<1..422)
                     /locus_tag="AC16FU_07140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR2994180.1"
                     /translation="MKILYLSFDPGIPYGGTKGSSIHIREFTKALRQAGHQVTTVVAR
                     LGAAAPKAGQVYKLPRVEADFFRPEAAWGSPALLAEAKAFAQNFAAKKLLKKLPAAHF
                     DFIYERYSLFGIAGLAWAREQQLPFLLEVNSPLLREAKT"
     gene            complement(419..1702)
                     /locus_tag="AC16FU_07145"
     CDS             complement(419..1702)
                     /locus_tag="AC16FU_07145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2994181.1"
                     /translation="MNPLRVGYFVKMYPRLSETFILNEILELERRGVEVIIFALRKPN
                     EGRFHPQVAEIKAPVFYLEELSPKKGWAVLAEHWTTLAPHSLQLWRLMEEVIGRDDPK
                     LLEALLAAAWATAVAVERGLGHLHAHFAVVPSTLAYFASRIAGLPFSFTAHAKDIFEN
                     TVDHQLLEEKLQAARFTITVTEFNRRYLTATYPAVPPDKIRVLPNGINLDFFNFEPNV
                     PRERSLILSIGRLVPKKGFPDLLEACAILKGKKIPFYSVIIGQGEDAPLLEAKRQELG
                     LTDVVTFVGPKTQVEVLPYLKRAAVLALPCTVDTDGNQDALPTVLLEALGTGCPVVST
                     TVSGIPEIIDSGSDGLLVPPATPEALAQALERLLLSPELAARFARQGRKKAEEKFDLK
                     KSVATLESYYRRSVETGTAPAAAEISLPVFGYKQS"
     gene            complement(1699..2964)
                     /locus_tag="AC16FU_07150"
     CDS             complement(1699..2964)
                     /locus_tag="AC16FU_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR2994182.1"
                     /translation="MKVKMAAPPFPAIPAERRLPSELARIVIYSHDTFGLGHFRRCLK
                     IARGFKSTYPQASILLVTGQPQALRYVLPEGTDFIKLPAVVKTGDNCYEPRSLSGSFE
                     SIFVLRQNLIFEAVRTFNPHLFLVDHSPLGMKGEIQKTLLWLKGERRRTAVVLGLRDI
                     LDEPEKVCATWRQESVYPILDETYDRIFIYGSPEVFDPVSAYQFGERARAKTSFTGYI
                     TDGARLSAGASEHRKGNGRKEVLVTIGGGEDGADIVTAYLEMLSRYPDQFNAQTTIVT
                     GPFLPEPQQKIFSQKASGLPVHLEEFIPELFPYLQRADLVIAMGGYNTVTEILAAARR
                     ALLIPRTHPRKEQLLRAQRLSELGLVSYIVPDRLTPDALYGTVTALLESDGEPLAQAR
                     RKNLLPLDGAPKLAQSCRSLLQRSAEVPR"
     gene            complement(2961..4367)
                     /locus_tag="AC16FU_07155"
     CDS             complement(2961..4367)
                     /locus_tag="AC16FU_07155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR2994183.1"
                     /translation="MATRSLTQPREVELLRGLALESDLPVYPATGIKVTVIGGGFVGL
                     VAAAGFAQFGHTVALVEKNSDKLNELKAGQVPIYERGLPQLLRANLSRQRLAVGAHMA
                     EAVKGSRAIFLAVGTPALPSGEADLSAVFEVMQNIAQTLEPKQVIVLKSTVPVGTAAQ
                     VRRVLAENGAREKQIAVVSNPEFLREGTAVYDFFRPQRIVIGGNSPEALELVAQLYRI
                     GMVEPCPIVVTNNETAEMIKYASNLFLATKVSFVNELALLCDAAGVNVTEVTRGMGLD
                     SRISPEFLNPGPGFGGSCLPKDLEAILSLAKKHGIDLFIAQAVRQANLRQPEKVVEKL
                     EQLVGSLQDKTIGVLGLAFKAHTNDLRDSPALAVMKLLKQKGAILQAYDPIATLDGKP
                     FGQEMRQVVSAAAAAAGAHALVVLTEWPEFLALDWEALGEKMKARNIVDARNLFVPEV
                     VVRHGFTYVGMGQKPEEV"
BASE COUNT          934 a         1302 c         1399 g          948 t
ORIGIN      
        1 gttttggcct cccggagcaa aggggaattt acctccaaaa ggaagggaag ctgctgttcc
       61 cgggcccacg cgaggccggc tatgccaaac agggagtagc gctcgtaaat gaaatcaaaa
      121 tgcgcggccg ggagtttttt caaaagtttt tttgcggcga agttttgcgc aaaggccttg
      181 gcttcggcca gaagagcggg tgagccccag gccgcctccg ggcggaaaaa gtcggcttcg
      241 actctgggaa gtttatagac ctgaccggct ttgggagcag ctgctcccag ccgggcgacg
      301 accgtggtta cctgatgccc ggcttgccgc aaggccttgg tgaactcgcg gatatgaatc
      361 gagctgcctt tggtgccccc ataggggatg ccgggatcaa acgacaggta cagaatcttc
      421 atgactgttt ataaccgaaa accggcaaag agatttccgc tgcggcgggc gccgttcccg
      481 tttccaccga ccgacggtag tagctctcca gtgtggcgac acttttcttc aggtcaaatt
      541 tttcttcggc tttcttcctt ccctgccggg cgaagcgggc ggccagttcc ggagagagca
      601 aaagcctttc cagcgcctgg gccagcgcct ccggggtggc cggcgggacc aggaggccat
      661 cgctgccgga atcgataatt tccggaatgc cggagacggt ggtggaaacc accgggcaac
      721 ccgtgcccag ggcctctaag agtacggtcg gcagggcatc ctgattgccg tcggtgtcca
      781 ccgtgcaggg cagggcgagc accgctgccc gcttcaggta cggcaggact tcgacctggg
      841 ttttcgggcc gacgaaggtc accacatcgg tgaggccgag ctcctgccgt ttggcttcga
      901 ggaggggagc gtcttcaccc tgcccgataa tgacggaata gaagggtatc tttttccctt
      961 tcagaatggc gcaggcctcc agtaaatcgg gaaaaccttt tttgggcacc agccggccga
     1021 tggagagaat caagctccgt tcgcgaggca cgttgggctc aaaattgaag aagtccaggt
     1081 tgatgccgtt gggcagcact ctgattttgt ccggcggcac ggccggatat gtggcggtca
     1141 ggtagcggcg gttgaattcc gtcacggtga tggtgaagcg ggcggcctgt aacttttcct
     1201 ccagcagctg gtggtccacc gtattctcaa aaatatcttt ggcgtgggcc gtgaagctga
     1261 acggcaaccc agcaatgcgg ctggcgaaat aggccagggt ggagggcaca acggcaaagt
     1321 gcgcgtgcag gtgtccgagc ccccgctcta cagcgaccgc ggtggcccag gcggcggcta
     1381 agagcgcctc gagcaacttg ggatcgtccc ggccgatcac ctcctccatg agccgccaga
     1441 gctgcaaact gtgcggagcc agggtcgtcc agtgctcggc taacaccgcc cagccttttt
     1501 tgggagagag ttcttccaga tagaagaccg gggccttgat ctccgccacc tggggatgga
     1561 aacgaccctc gttcggcttc cgcagcgcga aaatgattac ctccacgccg cgccgctcca
     1621 gttccagaat ttcgttcagg atgaacgttt ccgacagacg ggggtacatt ttgacaaaat
     1681 agccaacgcg taacggattc atcgtggcac ctcggcagaa cgttgcagaa gggaacggca
     1741 cgactgggcc agttttggtg caccgtccag cggcagaaga ttttttcggc gggcctgggc
     1801 caagggctca ccgtcacttt ctaagagcgc cgtgacggtt ccatacagag cgtcgggcgt
     1861 cagccggtca ggaacaatgt agctcaccag acccagttcg gacagtctct gcgcccgcag
     1921 gagctgctcc ttgcgggggt gggtacgggg gatgagcaag gctcggcgag cggcggccag
     1981 aatttcggtc acggtgttgt agccgcccat ggcgatgacc agatcggctc tctggaggta
     2041 aggaaaaagc tccggaatga attcctccag gtggaccggc agtccactag ctttttggct
     2101 gaaaattttt tgctgcggct cgggcagaaa aggaccggtg acgatcgtgg tctgggcgtt
     2161 gaactggtct ggatagcggg agagcatctc caggtaggcg gtgacgatgt cggcgccatc
     2221 ttcccccccg ccgatcgtca ctagtacttc cttccgtccg tttccctttc tgtgctcgga
     2281 cgcgccggcg gacagccgcg cgccgtcggt aatgtagccg gtaaagctcg tcttggcccg
     2341 ggctctttca ccgaactggt aggcggagac cgggtcaaaa acttccggtg acccgtagat
     2401 gaaaatccgg tcgtaggtct catccagaat cgggtagacg ctctcctgcc gccaggtggc
     2461 gcacaccttt tccggctcgt ccaagatgtc gcgcagaccc aagacgaccg cagtccgccg
     2521 cctttcccct ttcagccaga ggagcgtctt ctggatttcc cctttcatgc ccaaaggcga
     2581 atggtcgacc aaaaagaggt ggggattgaa cgtgcgcacc gcctcaaaaa tcaaattctg
     2641 ccgcaggacg aaaatgctct cgaaagagcc ggaaagcgag cggggttcgt agcagttgtc
     2701 cccggttttg accaccgccg ggagtttgat gaaatcggtt ccctccggca gtacgtagcg
     2761 cagtgcctgc ggctgtccgg tgactaggag aatcgatgcc tgcggatagg tacttttgaa
     2821 accccgggcg attttcaaac agcggcggaa gtgccccagc ccaaaggtgt cgtgggagta
     2881 aatgacgatc cgggccagct ccgacgggag tctgcgctcg gcaggaattg ccggaaaggg
     2941 gggggcggcc atcttgactt tcatacttct tccggttttt gccccatgcc cacataggtg
     3001 aagccgtggc ggacgactac ttccggcacg aagagatttc gggcatcgac gatgtttctg
     3061 gctttcatct tttccccgag cgcctcccag tccagagcca gaaattcggg ccattcggtc
     3121 aaaacgacta gggcgtgcgc cccggcggcg gcggcggcgg cggaaacaac ctgtcgcatt
     3181 tcctggccga agggcttccc atccagagtg gcgatcgggt cgtaggcctg gagaatggct
     3241 cccttctgct tgagtagttt cataaccgcc agcgctggag agtcgcgtag gtcgttggtg
     3301 tgggctttga aagccaggcc caggacgccg atcgttttgt cctgcagact gccaaccagt
     3361 tgttccagtt tctccaccac tttttccggc tggcgcaagt tggcctgccg gaccgcctgg
     3421 gcgatgaaaa gatcgatgcc gtgctttttg gctaaactga gaatcgcttc cagatctttg
     3481 gggagacagg atccgccgaa accggggccg ggattgagga actccgggct gatgcgggag
     3541 tccagcccca tcccgcgggt cacttcggtg acgttcacac cggcggcatc gcagagtagc
     3601 gccagttcgt tgacgaagga gactttggtg gccaaaaaaa gattggaagc gtacttgatc
     3661 atttccgcgg tttcgttgtt ggtgacgacg atcgggcagg gctccaccat cccgatccgg
     3721 tagagctggg cgaccaactc caaggcctcc ggactgttgc cgccgatgac gatccgctgc
     3781 gggcggaaga agtcgtagac cgcggttccc tcgcgcaaaa attccgggtt ggaaacgacc
     3841 gcaatctgct tctccctcgc tccgttttcg gcgaggacgc ggcggacctg cgccgcggtg
     3901 ccaaccggca cggttgattt caaaacaatc acctgcttcg gctccagagt ctgggcgata
     3961 ttttgcatca cttcaaaaac cgccgacaaa tcggcttcac ccgaaggcaa cgcgggagta
     4021 ccaaccgcca gaaagatcgc gcgcgagccc ttgaccgctt cggccatgtg ggcaccgacg
     4081 gccagccgtt gccgggaaag gttggcccgc aaaagttgcg gcagtccccg ctcgtagatg
     4141 gggacctggc cggctttgag ttcattcagc ttatcggagt tcttttcaac taaagcgacg
     4201 gtatgaccga actgggcaaa gccggcggcc gccaccaggc cgacgaaccc gccgccaatg
     4261 acggtcactt taataccggt ggcgggatag accggcaggt cggactccag agccagcccc
     4321 cggaggagct cgacctccct cggttgcgtt agcgaacggg tcgccattac aatttctctc
     4381 tcccggaata cgctgcgtca gctttttcag acaatgtcat ctttaataaa attcttgttt
     4441 cggagaattg tcggtcacta tctcttaaag gtcgaatcct cagactgggc aaagaattct
     4501 cctccaggct gtcaatggat ttgaaaaatt tgtttcgctg taagatcaag tagtatcaaa
     4561 aactatgccg aaaaagtatt tct
//
LOCUS       DBNGZA010000328        18319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2924749, whole genome shotgun sequence.
ACCESSION   DBNGZA010000328 DBNGZA010000000
VERSION     DBNGZA010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 18319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18319
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2924749"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16FU_07160"
     CDS             complement(<1..417)
                     /locus_tag="AC16FU_07160"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR2994184.1"
                     /translation="MFPLKDDIPSEKQPIVTIALIVANILAFILQLNSAYGFQTNMFR
                     MGLIPYEITHRVELNPEAPFPIYATLFTSMFMHGGFFHLGGNMLYLWIFGDNVEDQLG
                     HFRFLLFYLASGFAAFSIFFLTSPNSQTPLVGASVAI"
     gene            complement(561..1802)
                     /gene="icd"
                     /locus_tag="AC16FU_07165"
     CDS             complement(561..1802)
                     /gene="icd"
                     /locus_tag="AC16FU_07165"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HIR2994185.1"
                     /translation="MKFESLAPPAAGHRIEVKKNELIVPPDPVIPYIEGDGVGRDIMR
                     ATRTVVDAAVEKTYGGNRHLVWFEIFAGEKARDRFGQWLPKETIAAIKHYIVALKGPL
                     TTPIGGGFRSLNVTLRQVLNLYACIRPVRWFPGVPAPVKHPDKMNVVIFRENTEDVYA
                     GLEWPKGSKEAKKIISFLNKQLKVKVRADSGIGIKPISVTGTQRLVRKAIQYALANQK
                     KSVTLVHKGNIMKYTEGAFAQWGYAVAEKEFRNQIVTEVEVEQKFSGRRPEGRLLIND
                     RLSDSMFQQVLLRPDEYDILATPNLNGDYLSDACAAQVGGLGMAPGANIGDSLGLFEA
                     THGTAPKYTDKDMVNPCSLILSACFMLEYLGWKEAAQRITAGIERTIQQKKVTYDLAR
                     LMDGATKVSTSQFAKEVIKDM"
     gene            complement(1886..2821)
                     /locus_tag="AC16FU_07170"
     CDS             complement(1886..2821)
                     /locus_tag="AC16FU_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR2994186.1"
                     /translation="MKRSGHIPWGKLRVGLLLLVALLLFLWAAFTGGFFKPLQHYYEL
                     RTAFTNVQGLVTGSPVWVNGLEVGQVKSIALAPHLQENQVEVTVRLKREVWPYLRTDS
                     KIRVGTIGLLGDKYIEIFAGSDTATKLPPGAFLPAEKPVDAQAMFAEATESLRRLTPL
                     LDDIAAMTRKMRSGRSSIGRLIDDQKLYDDLVEATGAITRVVKNIDRGQEQALTRMAN
                     AAASLDTLAKKLYRGEGSAGKLVTDTALYTSLAHVAGQADTLFGKIGRGEGALGKLTQ
                     EEQLYAEISKLVTELDRLVTDIKAHPKKYFKFSIF"
     gene            complement(2846..3640)
                     /locus_tag="AC16FU_07175"
     CDS             complement(2846..3640)
                     /locus_tag="AC16FU_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994187.1"
                     /translation="MIAVERINYTIGDHQILRDVSLTVAAGETVVIIGPSGSGKSTLL
                     KIILGLAVPDSGRVFIAEQELTRARGQRLNDLRQQIGMVFQEGGLFDSLTVGENVGYY
                     LFEHTQMKEAEIEQVVRQTLATVGLDEDLIDAMPHTLSGGMQRRVAIARALAAHRPKV
                     MLYDEPTTGLDPISVEMVTECIAGLQQKLNLATVVVTHEIADALKIGSRFLVLNEGAV
                     VFQGNPDELARTDQPFVQQFLLPFRETLALAAEHFQVGPNNHGRNV"
     gene            complement(3637..4404)
                     /locus_tag="AC16FU_07180"
     CDS             complement(3637..4404)
                     /locus_tag="AC16FU_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR2994188.1"
                     /translation="MERFKKFLLGLQEIFFFAIRMFGALLRPPWYFRETYEQLYFIGV
                     GSLFLIVLTGIAAGQALALQTTKELADFGAKDYIGRIMTISVLRALGPVLTGLMVAAR
                     VASGITAEIGAMRSSNQIDALIAFGTDPIKKLAVPRLVSLLIMLPALTLITDAIAITG
                     GFLIAKFISHVSAHLYWANVKQKLTYGNFLVGIVKPFAFAIIIAAVACYKGFTARGGT
                     KGVGLATMESVVISSITILVVDFLLTKVVFSFLGWSA"
     gene            4506..5492
                     /locus_tag="AC16FU_07185"
     CDS             4506..5492
                     /locus_tag="AC16FU_07185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR2994189.1"
                     /translation="MRKQFEIRTVVLYFSEMNGGSCKLIINPAAGFGRTQKLLPRIIA
                     ALERQGIAYQFVTTTRAGEATGLARQAAFDGFHTVVAVGGDGTVNEVAVGLVDSNAAL
                     GVIPTGNGNDFARSVKIPLDLSPACQHLRTGQTVTIDVGRVNHQFFFNEVGVGLVGDV
                     ARLAQMQKRGPGKWRYVIAALKALRTFAPFKVSLKIDQVKLEKKIALLSIGNGTSTGG
                     GFRLTPDASPADGRLDLCVVAAASPWYLLRNLTKAYRGRHTQLPVTTIFRARHIEIAS
                     EQPLPTHVDGEVLSPAPTLLSVTASSHRLKVIAPAPVKTPETPQVRQQAAHA"
     gene            5485..5997
                     /locus_tag="AC16FU_07190"
     CDS             5485..5997
                     /locus_tag="AC16FU_07190"
                     /inference="COORDINATES: protein motif:HMM:NF040144.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR2994190.1"
                     /translation="MRRVSFLFFLLAWMAVSGQSQTLDTARNQSKPGPRRSPTGALLR
                     SVAFPGWGQLYNRKYFKALLLGGTEIGFGISAGVQWHRRDVHKKRFDSATDPGVRAFE
                     ESQFQFYEDNRNLFLWLVAGTIFYSMLDAYVDAHLAQYTEEGLPPLSLDYKPEEWGWG
                     VISLRLRFVW"
     gene            complement(6244..6915)
                     /locus_tag="AC16FU_07195"
     CDS             complement(6244..6915)
                     /locus_tag="AC16FU_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994191.1"
                     /translation="MALCLHLVKVAVESKGMLSNGLKRGFPPERLRSQTEPKVKRDDG
                     GYFIFTLNEGVKVYFEEYYHFLEQLESRAHTEQKNLIHKINHTDPEHLETLSFYRARK
                     VIIDLVLRTIYSFYTDGNNLGVIMSPWCFGTVVLEKAEIYREKIARGEVQDQNIPDYP
                     YYVLKYLDEIYKRTLLELFGFPEKAFSVRWQYTELLKRYSKVLTGVTSSLQSVLLSLK
                     HFGGT"
     gene            complement(7196..8182)
                     /locus_tag="AC16FU_07200"
     CDS             complement(7196..8182)
                     /locus_tag="AC16FU_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="HIR2994192.1"
                     /translation="MILPPSLSFAQTPTPLEKLRFDHPAQVTLYLKRDDLTGSVLSGN
                     KVRKLEFIFAEILKQKVDTVITCGGVQSNHARATSILAAKFDLKTLLFLHPIPENSQA
                     SDGNLFLDRLAGAEVRFLSDHQYLSVDRLMEEAKTVLERQGHKPYIIPEGASSELGVW
                     GYIIATKELKAQCQKIRLKPDRIVTAVGSGGTYAGLLIGTKLLKWPVRITGFNVCYDA
                     PYFKNRISGLIERTLAVYRLKVKCKPDELDIRDGYVGAGYARSRTEELALIHEVARQT
                     GVILDPTYTGKAMFGLLDQLQKGKISNKEKIIFLHTGGLFGLFPLRDKIPGI"
     gene            8355..9605
                     /locus_tag="AC16FU_07205"
     CDS             8355..9605
                     /locus_tag="AC16FU_07205"
                     /inference="COORDINATES: protein motif:HMM:NF017505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HIR2994193.1"
                     /translation="MTRQIVKFGFGLIFLAGTVVVSVQCAKKAAQVSAGDKYLFVWTG
                     DQARKAPDFLAVINFDQNAPDYGKVITTVPLPNPGNTWNEPHHVGLSRDGKVLACGGL
                     LSILKGQKEVFFFDVSDPAQPKFLSAADPPQSGVADEFYALDNGGYLVTMMGGAEGHH
                     PGRVAEFDKDLNLVAEYPQDPPQDGFNPHGVSVRPEVNLMVTSDFICPSTTLHAVPGD
                     PGFRGSVRVWDFQKRTILRTITLPNNAAGTIDVRLIPQDPQKRGYTAGMTDDKLYLLD
                     TQAGTAKPVFDFATIAKGGWPQLMRMTKDGKRLFISMNMAGKVVMFDTSDPEKPEVIK
                     VLELGEGSGPHYIALTPDEKRLVITDYFLNEDDAGKVHAEGDHKIHVAVVSANDLVLD
                     PKFRLDFNTAIATGPARPHGVAFK"
     gene            9776..10672
                     /gene="speB"
                     /locus_tag="AC16FU_07210"
     CDS             9776..10672
                     /gene="speB"
                     /locus_tag="AC16FU_07210"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR2994194.1"
                     /translation="MPNFLGIPDRFSNPDRARFLILPAPYEGTVTYKKGTKDGPAAII
                     AASQQVELFDEELGFEPYRAGIATLAALEVTPEGPKQMVERARRTVRELLRKKRMVIF
                     LGGEHSLTTGAVRAYQEKFNDLSVLQLDAHADLRDTYDNSPYNHACTLRRVREICPAV
                     QVGIRNLSAEENKFISQKGIQLYYAKDIWNTNDWIDEAIGKLPRNVYVTIDLDVFDPS
                     CMPAVGTAEPGGLSWYQVLEVLRKTVEEKNVVGFDVMELSPIPEMVAPDFLAAKLVYK
                     MIGYLMAKERILGRQVKPKHRR"
     gene            10787..12103
                     /locus_tag="AC16FU_07215"
     CDS             10787..12103
                     /locus_tag="AC16FU_07215"
                     /inference="COORDINATES: protein motif:HMM:NF037626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994195.1"
                     /translation="MKAPVSENMVGKSGSTVFPSVKAIRQRFEQAGTVKRVLQAVTGD
                     EKKVKVTGLSGSAVAFLTVALFEHHKKPLCFLTSDPDSAEKAAADLAALLPGEAVHLF
                     PERGVVPYEFRAPHEEVVGRRLQTLFALLRHEHSLVVASLPAVLEQTIPPEKLKAQSL
                     FLRPDDQIDLDEVARRLLALGFSRVPLVEEVGGFAIRGGIVDVFPCTAEDPLRLEFFG
                     DRLDSIRFFSVLDQRSITRAGSALILPQKEFYLPPAQLEEHLEKLDPRLEAEIREKFA
                     YEPDQPGIEWLAPLFGLERAPLFDYFPENFLLVVDEGETIKSKLGEIFEQAEKLSTEL
                     KRRLPLQLSPQSLYGAPEQVWMNIESYAGVEKLPTGQEVEGVIDLETKDQPAFGGKIE
                     FVRAKVRELGQYQYQTFITCETPGQAERLTELLVPMEEEIFLCTPS"
     gene            12119..14221
                     /gene="mfd"
                     /locus_tag="AC16FU_07220"
                     /pseudo
     CDS             12119..14221
                     /gene="mfd"
                     /locus_tag="AC16FU_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
     gene            14282..15994
                     /locus_tag="AC16FU_07225"
     CDS             14282..15994
                     /locus_tag="AC16FU_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2994196.1"
                     /translation="MGISVFAAVAWVAGCGRKPDENQAVALVGNHKIPLKQVLDRYPP
                     GTPFASAEEELNQKKALVQGLVEQVLLAEAGHRAGYDTLPEIVQQFEQEKDRTLIDAL
                     YETEVRKPVKVTEKDVKDWYDHLTEERRVSLIQVATQATAESLYQEIKKGKDFATLAL
                     ERSLDRNSNVKGGDIGFVQWGTIAQPLQEQVFSQGVGEVSRPVAVQKFWYLVKVVEKR
                     PFPRPDYGSARASLRQQLVAQRETDRANVFLDQLRTESAIVVDTAVAHEANRKLRELE
                     HGESPTGPVLRTFLDLSKVPASLGERPLAWCSGETLSVLKAYQALQGIMPLSRPHLET
                     EAQIKEIAFQIFLPQLLQAQARARELDRSARYREMLADLKDNLVAGVMRQKLIDAVHF
                     TDAEVRASYESRKPQYTQPAEFHLREIQLADSAEAVSLKKQIRSPEDFARFASAKTLR
                     PGYREKGGDIGYVRRESAPLFFAVVEKLAVNQVSSPVKNPDGTYSLLLLVERFPQKIT
                     PFDSVAHGIADTLLAERSRKLLDDWVAERQKTTKVTIDEDLLKKTIDYKWYAKKKESP
                     PVAG"
     gene            16011..17360
                     /locus_tag="AC16FU_07230"
     CDS             16011..17360
                     /locus_tag="AC16FU_07230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR2994197.1"
                     /translation="MKEKTARLFLLAGFIAALAAAAPASAGSVLDRIAAIVGNQIILE
                     SELKTQLALYAAQTGISLADSARLASLRSDLLEQMVDDRLVLQAAEKDTTLKVNPREI
                     DAALDEHIQKLKSQYPTEEAYQAQLAREGLSESDLRKRYRPEVRNQLLKQRLLQAKTA
                     KIGISSEEIQKFFERYRDSLPAQPASVKLAHILFAAEPGQRTIDSLRSRAESVLLQLK
                     EGADFAQLAKKYSDDPSGQNGGELGTFGRGEMVPEFEKAAFALKSGELSGIVETQFGY
                     HIIQSEGTDGQKVKARHILIAVKPSERDLEKAQALADSVYRLILRGADFADMAKRFSA
                     DDDSKNKGGELGWYAVADLTPEFKQAIDSLVADPAKGGTAGQVSRPTRSQFGFHLLKL
                     LERREGKKLALPEDYDTIKDLARREKTNQWLANWIKELKKKTYIEIKPASVAPAKAG"
     gene            17550..>18319
                     /locus_tag="AC16FU_07235"
     CDS             17550..>18319
                     /locus_tag="AC16FU_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppY/LpqO family protein"
                     /protein_id="HIR2994198.1"
                     /translation="MRRSTVTPRRVIPYIILFCCLCAVSSATADIDFGRIEKIVGATG
                     QVQDSVLVIRFPRSDLKVTIDGEPMPTALGFVSWTAWKRMGNAATVMGDLVLLEREIN
                     PVISAPAEANIEVTALHNHFLGEEPRILFMHIHGMGDADTLARGIHSALSKTATPAPV
                     SGATSTPPPALDTKKIEEIIGHPGQASGGVFKITIGRPGVIADGIEVTSTMGLNSWAG
                     FVGTNERAHVAGDVAMTTGEVNRVIRALRSGGIDVVAV"
BASE COUNT         3999 a         5084 c         5399 g         3837 t
ORIGIN      
        1 aatggctacg gaggcgccca ccaaaggagt ctgggaattg ggcgaggtca aaaagaaaat
       61 ggaaaaggcg gcaaacccgc tggcgagata gaagagcaaa aaacggaagt ggcccagctg
      121 gtcttccacg ttgtccccga agatccagag gtagagcatg ttgcccccca ggtggaagaa
      181 gccgccgtgc atgaacatgc tggtgaagag ggtggcgtaa atcggaaagg gggcttccgg
      241 gttcagttcg acccggtgcg tgatctcata ggggatgagc cccatccgga acatattcgt
      301 ctggaagccg taggcgctgt tgagttgcag gatgaaagcg aggatgttcg caacgatgag
      361 ggcgatagtg acaatcggct gcttttcact cgggatgtcg tctttgagcg ggaacataag
      421 gtcaggtttc cgatttttgt aagtgctgtg atggaaaagt acaggaagga tggcagggtg
      481 tcaatcattt ttggtggaga aaagtgaata actttcaaag cgacagagag aatttcgaga
      541 aaagaaattt tttcagtctc ctacatgtcc ttaatgacct ccttcgcaaa ctgcgaggtc
      601 gaaaccttgg tggcaccgtc catcaagcgg gcgaggtcgt aggtcacctt cttctgttga
      661 atcgtgcgtt caatgccagc ggtgatcctc tgggcagcct ctttccagcc gaggtattcg
      721 agcataaagc aagccgagag aatgagcgaa cagggattga ccatatcttt gtcggtgtac
      781 ttgggagccg tgccgtgggt ggcttcgaac aagccaagag aatcgccgat gttggctccc
      841 ggcgccatcc ccaggccgcc cacctgggcc gcgcaggcat cggagaggta gtcgccgttc
      901 aaattcggtg tggccaggat atcgtattcg tcggggcgca gcagtacctg ctggaacatc
      961 gagtcggaca ggcggtcatt tatcaagagc cggccctcgg gccgccggcc gctgaacttt
     1021 tgctccacct ctacttcagt gacgatctga ttccggaatt ctttttcggc gactgcgtag
     1081 ccccactggg caaaggcgcc ttcggtgtat ttcataatgt tgcccttgtg caccagggtc
     1141 acgctcttct tttggttggc cagagcatac tgaatggctt tgcgcaccag gcgctgggtt
     1201 ccggttaccg aaatcggttt gatgccgatg ccggagtcgg cccggacttt cactttcagc
     1261 tgtttattca aaaaggaaat gatttttttg gcttcttttg aacctttggg ccattcgagc
     1321 ccggcgtaga cgtcttcggt gttctcgcgg aagatgacca cgttcatctt atccgggtgt
     1381 ttgaccgggg cggggacgcc ggggaaccag cgcacgggcc ggatgcaggc gtagaggttc
     1441 agtacctgtc gtaaagttac gttcaaactg cggaaaccgc cgcctatggg cgtcgtgagc
     1501 ggccccttca acgccacaat gtagtgtttg atggccgcaa tagtttcttt gggtagccac
     1561 tggccgaagc ggtcgcgggc tttttctccc gcgaagattt cgaaccagac gaggtgtcgg
     1621 ttcccgccgt aggttttctc gacggctgca tcgacgaccg ttcgggtggc ccgcatgatg
     1681 tcgcggccca cgccgtcgcc ttcgatatag ggaataaccg ggtcgggcgg aacaatcagt
     1741 tcattctttt tgacttcgat ccggtgtccg gcagcgggcg gagcgagaga ttcgaatttc
     1801 atggcggtaa aataagcaaa cggcgagttg gacgaaagtc gaatcagaaa atccgataca
     1861 tttaatccgc ttgctcaagc ggcaatcaaa agatgctgaa cttaaagtat ttcttcggat
     1921 gggctttgat atcggtgacc agccggtcca gttcggtcac taatttggag atttcggcgt
     1981 acagttgctc ttcctgggtc agttttccca gagcgccctc tccccggcca attttgccga
     2041 aaagggtgtc ggcctgcccg gccacgtggg ctaaactcgt gtacagggcg gtgtcggtga
     2101 cgagcttccc cgcactgcct tcaccgcggt agagtttctt ggccaaagta tcgagcgacg
     2161 cggcggcatt ggccatccgg gtgagcgcct gttcctggcc tcgatcgatg tttttgacca
     2221 cgcgcgtgat ggccccggtg gcttccacca gatcgtcgta cagtttctgg tcatcgatca
     2281 gccggccaat actgctgcgg ccgctgcgca tttttctggt catggccgcg atatcgtcca
     2341 gcagcggcgt gaggcggcgc aggctttcgg tggcctcggc gaacatcgcc tgggcatcga
     2401 ccggtttttc ggcaggcagg aaggcgcccg gcgggagctt cgtcgcggta tcggaaccgg
     2461 cgaaaatttc aatgtacttg tcgcccagaa gcccgatggt gcccacccgg attttggagt
     2521 cggtgcgcag atacggccag acttcgcgct tgagccgcac cgtcacctca acctggtttt
     2581 cctggaggtg cggtgccaga gcgatcgatt tcacctggcc tacttccagc ccgttcaccc
     2641 agacgggcga gccggtcacc aggccctgca cgttggtgaa ggcggttctc agttcatagt
     2701 aatgctgcag gggcttgaaa aaccctcccg tgaaggcggc ccagaggaaa agcaggaggg
     2761 cgaccagaag aagcaggccg acccgcagtt taccccaggg aatgtggccg gatcgtttca
     2821 tggtcttcgc tgccgcgtca gttgctcaaa cgttccgtcc gtggttgttc ggccccacct
     2881 ggaagtgttc ggcggccaac gccagcgttt cccggaaggg caggagaaat tgctgcacga
     2941 agggctggtc ggtgcgggcc agttcgtccg gattaccctg aaaaaccacg gcaccctcgt
     3001 tcagcaccag aaagcggctg ccgattttca aagcatcggc aatctcgtgg gtgacgacca
     3061 ccgtggccag attcaatttc tgctggagac cggcgatgca ctcggtgacc atttccacgg
     3121 aaatcgggtc taaaccggtg gtgggctcgt cgtagagcat caccttgggc cggtgcgccg
     3181 ccagcgcccg ggcaatggcc acccgccttt gcatcccgcc ggaaagggta tggggcatgg
     3241 cgtcgatcaa gtcttcgtcc agccccaccg tggccagggt ttgccgcacc acctgttcga
     3301 tttcggcttc tttcatctgc gtgtgctcaa agagataata gccgacgttc tcgccgacgg
     3361 tcaaggagtc gaacagcccg ccttcctgga aaaccatgcc gatctgctgg cgcaagtcgt
     3421 tcaacctctg gccgcgagcg cgggtcagtt cctgttcagc gatgaagacc cggccggaat
     3481 cggggacggc caggcccaag ataattttca gaagcgtgct cttgcccgat cccgacggcc
     3541 cgatgatcac cacggtttcg cccgctgcca ccgtcaggct gacgtcgcgc agaatctggt
     3601 gatcgccgat ggtgtagttg atgcgttcga ctgcgatcat gcgctccatc ccagaaagga
     3661 gaagaccacc ttcgttaaca ggaaatccac caccagaatg gtgatggaac tgatcaccac
     3721 cgactccatg gtcgccaggc cgacgccttt ggtgcccccg cgggcggtaa agcccttgta
     3781 gcaggctacc gcggcgatga tgatggcgaa ggcgaacggt ttgactatgc cgaccaagaa
     3841 attgccgtag gtcagtttct gcttgacgtt ggcccagtag aggtgggccg aaacgtggga
     3901 tatgaatttg gcaatcaaaa agccgccggt gatggcgatg gcatcggtga tcaaagtcag
     3961 ggccggcagc ataatcaaaa gactcaccag gcgcgggacg gccaactttt tgatcgggtc
     4021 ggtgccgaag gcgatcaaag cgtcaatctg gttcgaagag cgcatggcgc cgatttcggc
     4081 ggtgatgccc gaagcgaccc gggcggccac catcagtcca gtcagtaccg gtcctaacgc
     4141 gcggaggacc gaaatggtca taatccggcc gatgtagtct ttggcgccga aatcggccag
     4201 ttccttggtc gtttgcaggg ctaaggcctg cccggcggcg atcccggtga gcacgatcaa
     4261 aaagagcgag cccaccccga tgaagtacag ctgctcatac gtctcacgga agtaccaggg
     4321 cggacggagg agtgcgccaa acattcggat agcaaaaaag aagatctcct ggaggccaag
     4381 gaggaatttt ttgaaacgtt ccattgctgc ttacgctcac ccttcgtttt caggaaaata
     4441 gggacacacg cgagaaaaac caaccggccc tgccgtgcgg aagttgcctg cacgacaccc
     4501 ggcgtatgcg gaagcagttt gaaatacgta cggttgtgct ctattttagc gagatgaacg
     4561 gcggctcgtg caaacttatc attaatcccg cagccggttt tggccggacg cagaaactcc
     4621 tgccccgcat catcgctgct ctggaacggc aggggatcgc gtaccagttc gtcaccacta
     4681 cgcgggccgg ggaagcgacc ggcttggccc gccaggccgc cttcgacggt tttcacaccg
     4741 tggtggccgt ggggggagac gggacggtga atgaagtcgc tgttggtctg gtcgatagca
     4801 atgccgcgct gggtgtcatc cccaccggca atggaaacga ctttgcgcgc agcgttaaaa
     4861 tccccttgga cctttcacca gcatgccagc atttgcgcac cggccagacg gtgacgattg
     4921 atgtgggccg ggtgaaccac cagttcttct tcaacgaagt cggtgtgggg ctggtgggcg
     4981 acgtggcccg gctggctcag atgcagaagc gggggccggg gaaatggcgt tacgtcattg
     5041 ccgcgctgaa ggcgctccgg acttttgctc cgttcaaagt ctcgctgaaa attgaccagg
     5101 tgaaactgga aaagaaaatc gccttgctca gtatcggaaa cgggacctct accggcggcg
     5161 gctttcgcct gacaccggat gcttcccctg ccgacggacg gttagatctc tgcgtggtgg
     5221 ccgcggcgtc tccctggtac ctcttgcgca acctgacgaa agcctatcgc ggccggcaca
     5281 cgcaactgcc cgtgaccacc atcttccgcg cccggcatat cgaaattgcc tcggagcagc
     5341 cgctgccgac ccatgtcgac ggcgaggtct tgagcccggc gccgacactt ctgtcggtaa
     5401 ccgcctcgtc ccaccggctg aaagtgattg cgccggcccc ggtcaaaact ccagagactc
     5461 cgcaggtgcg gcaacaggca gcgcatgcgt agggtaagtt ttcttttttt tcttctggcc
     5521 tggatggcgg tttccggcca gtcccagact ctggatacag caaggaatca atcaaagccg
     5581 gggccgcgcc gctcgcctac cggtgcgctt ctgcgctcgg tggcctttcc cggctggggg
     5641 caactctata accggaagta cttcaaagcg cttctcctgg gcgggaccga gatcggtttt
     5701 ggtatttcag cgggtgtgca gtggcaccgg cgcgacgtgc acaaaaagcg cttcgattcc
     5761 gccaccgatc cgggggtgag agcgtttgaa gaaagccaat ttcagttcta tgaggataac
     5821 cgcaacctct tcctgtggct cgtggccggg acgattttct actcgatgtt ggacgcctac
     5881 gtcgatgcgc accttgccca gtataccgag gaaggcctgc cgcccctgtc gctggactac
     5941 aagccggaag agtgggggtg gggagtgatt tcgttgcggc tgagatttgt gtggtagctg
     6001 ttctgcgctg tattcactcc tgatgggcaa gcaaccccct cctgatattt acgccatcga
     6061 gtgcgagagg cgcctttgcg gttaaaacag cgtattcgat gagacctaaa aatagaaggg
     6121 cggttgcggc aaccgccctg tcccgcactt ttggcgggac ctccccgatt ggtcgggggt
     6181 tcagcgtccg aaactctttc ccaatcgtag ggcgagcgtc ctcgctcacc ctccagcgga
     6241 ttcttacgtc ccgccgaagt gcttcagcga aagcaaaacg ctctggagcg aactggtcac
     6301 gccggtcaag accttcgagt agcgcttgag cagttccgtg tactgccagc ggaccgaaaa
     6361 cgccttctcc gggaagccga ataactccaa gagcgtccgc ttgtagattt cgtccaagta
     6421 cttcagcaca tagtaggggt agtccgggat gttctggtcc tgcacctcgc cgcgggcgat
     6481 cttctcccgg tagatttcgg ctttctccag caccaccgtg ccgaagcacc agggggacat
     6541 aatcacgccc aggttattcc cgtcggtgta gaacgagtag atggtccgca gtactaaatc
     6601 gatgatcacc ttcctggcac gatagaaaga gagggtctcc aggtgctcgg gatcagtgtg
     6661 gttaattttg tgaatcagat ttttctgctc ggtatgcgcc cgggattcga gctgctctaa
     6721 aaagtgatag tactcttcga agtaaacctt cactccctca ttgagggtga agatgaagta
     6781 gccgccgtca tctcttttta ctttcggctc ggtttgcgag cggagacgtt cgggcgggaa
     6841 cccgcgcttg agcccattgg agagcatccc tttcgattcc acggcaacct tcaccaagtg
     6901 caaacatagt gccattacaa agacggcctg aagcggcgca aaacgagatg gcgtcgggga
     6961 gcggtttaac taacccgttg gtagaaaaag aattacggat cgcctgaaaa gtagagttaa
     7021 gaggccatta cgcctatgtc gtacgactta aatagaaaca gatactgaca ccggcccggc
     7081 ctgcaaacga gttcacagat tgggaatgga ggaggcatga tcaattgcgt tgagcaagca
     7141 ttcttttggt tgaatgtttt ttctcgcagc cgacgtcgga tgtctcggat cgtatttaga
     7201 ttccgggaat cttgtcgcgc agcgggaaaa gaccaaagag gccgccggtg tgcaggaaga
     7261 tgattttttc tttgttactg attttgcctt tttgcagttg atccagaagg ccaaacattg
     7321 cctttccggt gtaagtcggg tcgaggatta ccccggtctg ccgggccact tcgtgaatca
     7381 gggccaattc ctcggtccga gagcgggcat agccggcgcc gacatagccg tcgcggatat
     7441 cgagttcgtc cggtttgcat ttaaccttga gccggtagac agcgagcgtg cgctcaatta
     7501 ggccggaaat ccggttcttg aaataggggg cgtcgtagca gacgttgaag cccgtaatgc
     7561 ggaccggcca cttcaaaagt ttcgtgccga tcaaaagccc ggcgtaggtg cctccggagc
     7621 cgacggcggt gacgatccgg tctggtttga ggcgtatttt ttggcactgg gctttcagtt
     7681 ctttggtagc gattatatac ccccagaccc ccagctcact cgatgcgccc tcaggaatga
     7741 tatagggctt gtgtccttgg cgctccagga cggttttggc ttcctccatc agccgatcaa
     7801 ccgaaaggta ctggtgatcg gagaggaagc ggacttcggc tcccgcgagc cggtcgagaa
     7861 agagattgcc gtcagatgcc tgcgagtttt ccgggatggg atggaggaaa agcagagtct
     7921 ttaggtcgaa tttagcagcc agaatcgagg tggctctggc gtggttggac tgcaccccgc
     7981 cgcaggtaat gacggtgtcg actttctgct tcagaatctc cgcgaatatg aattccaact
     8041 tacgcacctt atttccggaa aggacggagc cggtgaggtc atcgcgcttg aggtagagcg
     8101 ttacctgcgc cggatgatcg aaccgtaatt tttccagcgg ggtgggagtt tgggcaaagg
     8161 agagagaggg tggcaaaatc ataaggatag gatataaaga tcggcccgtc tgtcaaagga
     8221 gacaaaaaga gtcttgacaa acgcaatggt ttgtacttaa atatttcaaa gggaatggga
     8281 tggttcttcc cgaaaaatcg gaaagaacca ggagtgaaat ttctgtaact ggatgggcca
     8341 aaggagggtg ggtcatgacg aggcaaattg taaagttcgg attcggccta atttttctgg
     8401 cgggaacggt ggttgtatcg gttcaatgcg cgaagaaagc cgcccaagta tctgcgggag
     8461 acaaatactt gttcgtctgg acgggagacc aagcgcggaa agcccccgac tttttagcgg
     8521 tgatcaactt cgaccagaac gcaccggact acggcaaggt catcactact gttcccctgc
     8581 ccaatcccgg aaatacctgg aacgaaccgc accatgtagg gctttcccgt gacggcaagg
     8641 ttctcgcctg cggcggcctt ttgagcattt tgaaagggca gaaagaagtt tttttcttcg
     8701 atgtatctga tccggctcag ccgaaatttc tctcggcggc tgatccgccc caatcgggtg
     8761 ttgcagacga gttctatgcg ctggacaacg gtgggtattt ggtcacgatg atgggcgggg
     8821 cagaggggca tcatcccggt cgtgtcgccg agtttgataa agatttgaac ttggtggccg
     8881 aatatcccca agaccccccg caggacggtt ttaatccgca cggggtatcg gtccgcccgg
     8941 aggtcaatct catggtcacc agtgacttca tctgcccttc caccacgtta cacgcggttc
     9001 ccggcgaccc gggattccgg ggcagcgtgc gggtctggga tttccagaag cggactattc
     9061 tacgcacgat taccctgccc aacaatgcag ccggaacgat tgacgtgcgg ttgattccac
     9121 aagacccaca gaagcgaggc tacactgcgg gaatgaccga cgataaactc tacctgttgg
     9181 atacgcaagc cggaacggcc aaaccggttt ttgatttcgc aaccattgcc aaaggcggct
     9241 ggccgcaact gatgcggatg accaaagacg ggaagcggtt attcatctca atgaacatgg
     9301 cgggcaaagt ggtgatgttt gacacctctg acccggaaaa gccggaggtg ataaaagtgc
     9361 tggagttggg ggaaggttcc gggccgcact acatcgccct cactccggat gaaaagcggc
     9421 tggtgattac cgattacttc ttaaatgaag atgacgcggg caaagtccac gcggaggggg
     9481 atcacaaaat ccacgttgct gtggtctcgg ccaatgacct ggtcctcgac cccaagtttc
     9541 ggttggactt caacacagcg atagccaccg ggccggctcg tccacacggg gtggcattta
     9601 agtaatggcc cgaagtggat ggcgataaaa ttccgaggtg gacggactcc ttcgggaccc
     9661 gtccacctca ttaaatctct ccttcttatt caaaacacaa tttcccagcc acgtcttttt
     9721 tgaggatttc acttgacgga gggaatgatc gtcgttgtat tccagcaacg agtcgatgcc
     9781 caacttctta ggaattcctg atcgtttttc caaccccgat cgggcccgct tcctcatcct
     9841 ccccgcgccg tacgagggca cggtcaccta caagaaagga acaaaagacg gaccagcagc
     9901 catcatcgca gcgtcgcagc aggtggaact tttcgacgag gagttgggat ttgaaccgta
     9961 ccgcgccggc attgctacct tggctgcgct tgaagttacc cccgaggggc cgaagcagat
    10021 ggtcgagcgg gcccggcgga cggttcggga actgctacga aagaagcgga tggtcatttt
    10081 tctgggcggg gagcattccc ttaccaccgg cgcggtgcga gcgtaccagg aaaaattcaa
    10141 tgatctctca gtcttacaac tggacgccca cgccgacctg cgcgacactt atgacaattc
    10201 cccttacaac catgcctgca ccctgcgccg ggtgcgggaa atatgcccgg cggtgcaggt
    10261 gggcattcgc aacctcagcg ctgaagaaaa caaattcatt tcccaaaaag gaatccagct
    10321 ctactacgcc aaggacattt ggaataccaa cgattggatt gacgaagcta ttggaaagct
    10381 tccccggaac gtctacgtga ctatcgatct cgatgtcttt gacccctcat gtatgcctgc
    10441 cgttggtaca gccgagccgg gcgggctgtc ttggtaccag gtgctggagg tgctgcggaa
    10501 aaccgtcgaa gagaaaaatg tggttggttt tgacgttatg gagctgtcgc cgataccgga
    10561 gatggtggcg cctgactttc tggcggccaa actcgtatat aagatgattg gatacctgat
    10621 ggccaaggag agaattctcg gccgccaagt caaaccgaaa cacaggaggt aagagcggct
    10681 tattctttgt cccgtaaacc gttaacttgg aatgtcaccg attgtgaaca cccccggctc
    10741 cgcgcggagg cggggttttg gcgtttcggc tatgcgtggg aatagtgtga aagcaccggt
    10801 cagcgaaaac atggttggaa agagtggttc aaccgttttt ccatccgtca aggccattcg
    10861 tcaacggttt gagcaagctg gcacggtcaa acgggtcctg caggcggtta ccggagatga
    10921 gaaaaaagtc aaagttaccg gcctgtcggg ctcggctgta gcttttttga ccgtggctct
    10981 attcgagcac cataaaaaac cgctctgttt cctcacgtcc gacccggact cagccgaaaa
    11041 ggccgccgcc gacttggctg cgctattgcc gggagaagcg gttcacctct ttcccgagcg
    11101 cggggttgtg ccttacgaat tcagagcgcc tcacgaagag gtcgtgggcc gcaggctcca
    11161 gacgctcttt gcccttttgc gtcacgagca ttcactggtc gtggcctccc tgcccgcagt
    11221 gctggagcag accattcccc cggaaaagtt aaaggcgcag agcctctttt tgcgcccgga
    11281 cgatcagatc gatcttgatg aggtcgcaag gcggcttttg gcgctgggct tctcgcgcgt
    11341 gccgctggtg gaagaagtag gcggcttcgc catccgcggc ggcatcgtgg acgtcttccc
    11401 ctgcaccgcc gaagatcccc tgcgcctgga attttttggc gaccggctcg actcgatccg
    11461 cttcttttcg gtattagatc aacgttcgat tacccgtgcc ggttcagcac tgattttgcc
    11521 gcagaaagag ttctacctgc cgcccgcaca actcgaagag cacctggaga aacttgaccc
    11581 gcgtcttgag gcagaaattc gggagaaatt tgcctacgaa ccggaccagc cgggaattga
    11641 atggctggcc ccgctcttcg gattggagcg ggccccgctc ttcgattact tccccgaaaa
    11701 ctttttgctc gtggtcgatg aaggggaaac gatcaaaagc aagctggggg aaattttcga
    11761 acaagccgaa aaactctcca ccgaactcaa aaggcgattg ccgctccagc tttccccgca
    11821 gtccctctat ggtgctcccg aacaggtttg gatgaacatt gagtcgtacg ccggggttga
    11881 gaaacttccc acgggtcagg aagtggaggg ggtcatcgac ctcgagacga aggaccaacc
    11941 cgctttcggg gggaaaattg agtttgtcag agccaaagtg agagaactgg gtcagtacca
    12001 gtaccagacc ttcattacct gcgaaactcc cggccaggcc gagcgtttaa ccgaacttct
    12061 ggttccgatg gaggaggaaa ttttcctctg caccccctct taggccgaag gcttcctttt
    12121 ccccgagccg caactggcgg tctttaccga tcaccagatt ttccgtcggc cgcagcaccg
    12181 ctggcccaaa cgccgcttcc gggaaggagt ggcgctctcc gcctacacca atttgcagcc
    12241 gggcgattac gtggtccaca tcgactacgg catcggccgc ttcctgggga ttgagacgct
    12301 cattgtggaa ggccgcaagc gcgattgcct ggccattctc taccaggaag gggacaagct
    12361 ctacgttccc accgaagagt tcaaccgggt gcagaagttc atcggtcacg agggggaacc
    12421 caccctctca cgcctgcgtt ctgatacctg ggagaaactc aaagcccgca ccaaggaagc
    12481 gctcctggaa atggcgggag agttgatcgc cctctccgct gaacgcaaag ccaagcccgg
    12541 agccgctttt tctcccgata ccccctggca gaaagagttg gaggcagcgt tcatctatgc
    12601 cgataccccg gaccagctgg ccgccattga agcaattaaa agcgatctag aaaagccggc
    12661 accgatggac cggctggtct gcggtgatgt cggcttcggc aagaccgaag tggcgatccg
    12721 ggccgcgttt aaagcattga aggacggcaa aaaggtggct gttcttgttc ctacgacaat
    12781 tttagctcag cagcactacc tcaccttcag cgagaggctg cttccctctc cggtcaccct
    12841 cgccacactc tctcgcttca agagtagaaa ggaacaaaaa gaggtcattg aaaatctgaa
    12901 agcggggaaa ctggacatcg tcatcggcac gcaccggttg ctccagcggg atatccactt
    12961 ccacgacctg ggacttctgg tgatcgacga ggagcagcgc tttggggtga agcagaagga
    13021 gcggatcaag gacctgaagc gccaggtcga tgtattgacc ttaaccgcca cccccattcc
    13081 gcgcaccatg caactcgctc tctacggtgc ccgcgacctc tcggtcatca acacggcacc
    13141 gcaaggccgg ctgccgatcc acactgaaat catcgagcgc cgggacgact tggtggccga
    13201 cgccatcctg cgggagctgg cgcgtggcgg ccaggtcttc ttcgtccaca accgggtgca
    13261 gtcgattgaa tctatcggcc gctggctggg tcggatcgtg ccacaggcgc ggctcataat
    13321 agcgcacggt caaatgccgg agcaccggtt agaaaaagtg atgctcgctt ttctgaaccg
    13381 aaaatatgac ctcctcctgg ccacttccat aattgaaaat gggctggaca ttccctccgt
    13441 caataccatt atcatcgacc gggccgacac cttcggcctg gcgcagctct accagttgcg
    13501 cggccgggtg gggcgttcgc attacaaagc gtacgcgtac cttttaactc ccgccgcacg
    13561 ggagatgact gagaccgcgc gcaagcggtt aaaagccctg gcccagcacg gcgatctggg
    13621 ggccggtttc catctggcac tgcgcgactt agaaattcgc ggcgcgggca acctcttagg
    13681 cccccaacag cacggctata ttgaagccgt gggctttgaa ctttactgtc agcttctcga
    13741 agaagccgtg cgggagttga aaggagaaaa ggcggcgccc gaaataaagg tcaaaattga
    13801 agccgaatcc gatttcttcc tgccggaggc ctacgtcacc ctgccggccc agcgagtcga
    13861 gatctaccag aaaatcgccg gagtgaaaac cgagaaggga gtcgctgacc tgacggctga
    13921 gctggccgac cgttttgggc cgttgccgga ctcggttcag gcgcttttgg aactctcgcg
    13981 gttaaagctc gtggcgcagg cgaaatcgat ccagaaaatc cggctcaccc ccggctgggt
    14041 ggagtttgca ttcgcgcccg aagtgacggt tacgaaacaa acggttgaac gtctgcgccg
    14101 gacgatccct ttccccctgg aattttccag cgccggacgg ttcggttttc gggtgggggt
    14161 gccggacggg ccggggcccg cccggctggc gcaggtgaag tcactcttgc tcgccctcta
    14221 aagaacgtat actttgcctt agatgagtac tacccgaaag acaatctggt tgttagcatt
    14281 tttgggcatt tctgtttttg cggcggtggc ctgggtggcc ggttgcgggc gcaagccaga
    14341 cgagaatcaa gccgtcgctc tggtcgggaa ccacaagatt ccgctcaagc aagtcctcga
    14401 tcgctacccg cccggcactc cttttgccag tgccgaagaa gaactgaacc agaaaaaagc
    14461 tctggtgcag gggctggtgg agcaggtgct tttggcggaa gccggccatc gagccgggta
    14521 tgatactctc ccggagattg tccagcagtt cgagcaggaa aaagaccgca ccctcattga
    14581 cgctctctac gaaaccgagg tgcgcaagcc ggtcaaagtc acggaaaagg acgttaagga
    14641 ctggtacgat cacctgaccg aggaacggcg cgtgagcttg atccaggtag cgactcaggc
    14701 gacggccgag agtctctacc aggagatcaa aaaaggaaaa gattttgcga ctctggcttt
    14761 ggagcgctcc ctggaccgca attccaacgt caaaggcggc gacatcggtt ttgtgcagtg
    14821 gggcacgatt gcgcaaccgc tgcaggagca ggttttttct cagggagtcg gtgaggtgtc
    14881 gcggccggtg gcggtgcaga agttctggta cctggtcaaa gtggtcgaga aacggccgtt
    14941 cccgcgcccc gactacggga gcgcccgagc ctcgttgcgc cagcagctgg tagctcaacg
    15001 cgagacggat cgcgccaatg tctttctcga tcagctgcgc acggagtcgg ctattgtcgt
    15061 ggatacggcg gtggctcacg aggccaatag aaaactccgc gaactggagc acggtgaatc
    15121 gcccaccgga ccggtactgc ggaccttctt ggatctttcc aaggtacccg ccagtctggg
    15181 ggagcgaccc ctggcctggt gttccggcga aacgctcagc gtgctcaagg cctatcaggc
    15241 cttgcaaggt atcatgcccc tttcccgacc ccacctcgaa accgaagcgc aaatcaaaga
    15301 aattgcgttc cagattttcc tgccgcagct cctgcaggct caggcccgcg cccgggagtt
    15361 agatcgttcc gcccgttacc gggaaatgct ggccgacctg aaggacaacc tggtcgccgg
    15421 ggtgatgcgc cagaagttga ttgacgccgt gcattttacc gacgccgagg tgcgcgcctc
    15481 ctacgagtcg cggaagccgc agtataccca gccggccgag ttccacctgc gggaaattca
    15541 gctggccgac tcggccgagg cggtatcgct caaaaaacag atccgctcgc cggaggactt
    15601 tgcccggttc gcctccgcga agaccctgcg gcccggctac cgggaaaagg gtggcgacat
    15661 cggctacgtg cgccgggaat ccgcccccct cttctttgcc gttgtggaaa aattagcggt
    15721 gaatcaggtt tcttccccgg tcaaaaaccc ggatggcacc tattctcttt tgctgctcgt
    15781 ggagcgtttt cctcaaaaaa ttacgccgtt tgactcggtg gcccacggta tcgccgatac
    15841 cctgttagcc gagcgctccc gcaaattgct tgacgactgg gtggcggaac gccagaagac
    15901 caccaaggtg acgattgatg aagatctgct caaaaagacg atcgactaca agtggtatgc
    15961 gaaaaagaaa gagagtcccc cggtcgccgg ctaagatgtc ccctgcccct ttgaaggaaa
    16021 aaaccgcccg gctcttccta ttggccgggt ttatcgccgc tctggcggcc gccgcacctg
    16081 cgtcggctgg cagcgttctc gaccggatcg ccgccattgt tggtaaccag atcattttgg
    16141 aatcggaact aaagacgcaa ttggcgctct acgcggcgca gaccggcatc agtctcgccg
    16201 acagcgccag actggcttcc ctgcgctccg atctcttgga gcagatggtt gacgaccggc
    16261 tggtgctaca ggcggcggaa aaggatacta cgctcaaggt caacccgcgc gaaattgacg
    16321 cggccctgga cgagcacatc cagaagctca aaagccagta tcccaccgaa gaagcgtacc
    16381 aggcgcaatt ggcgcgggaa gggctcagcg aaagcgattt gagaaagcgc taccgcccgg
    16441 aagtgagaaa ccagctctta aaacagcgcc tgctgcaggc caaaacggcg aaaatcggca
    16501 tctccagcga agaaattcaa aagtttttcg aacgttaccg cgacagtctg cccgcccagc
    16561 cggcctcggt gaaattggcc cacatcctct tcgccgcaga gcccggccag agaaccatcg
    16621 actcactccg cagccgggcc gagtcggttt tgctccagtt gaaggagggg gccgactttg
    16681 cgcagttagc caaaaagtat tcagatgatc cctccggaca gaacggcggt gagttaggca
    16741 cctttggccg gggcgaaatg gtgccggagt tcgaaaaagc cgcctttgcg ctcaaatccg
    16801 gagagctttc cggcatcgtc gagacccagt tcgggtacca catcatccag tcggaaggga
    16861 ccgacggcca gaaggtcaag gcccggcaca ttttgattgc cgttaagccg tcggagagag
    16921 acctcgaaaa ggcgcaggcg ctggccgatt cggtctaccg cttgattctc cgcggcgccg
    16981 attttgccga tatggccaag cgcttctccg ccgatgacga ctccaaaaac aaaggcgggg
    17041 aactgggctg gtacgcggtg gccgatttga ccccggagtt caaacaggca attgactcgc
    17101 ttgtcgcaga tcccgccaaa ggcgggaccg caggccaggt aagccggccc acccgctcgc
    17161 agttcggctt ccatttgctc aaactccttg agcgccggga aggcaagaaa ctcgccctgc
    17221 cggaggacta cgatacgatt aaagacctgg cccgccggga gaaaaccaac cagtggctgg
    17281 cgaattggat taaggaactg aagaaaaaga cctatataga aatcaaaccg gcctcggtcg
    17341 ctccggcgaa agcggggtga ccaataactg caagttgccc gatttaaatc aaaacctctt
    17401 atttcctcac acattcacac ctttaggaaa aactaaacgt ataattgcgt aactgaacag
    17461 cgcttccttg aagctaactg ctggcgcatt gcaaaatgga caatctaaat gctgcagctg
    17521 gaaacttgtt ttgaaaagag gaagagcata tgcgaagatc aacagtaacg ccgagaagag
    17581 tgattcctta cattattttg ttctgctgtt tatgcgcggt tagctccgct accgctgaca
    17641 ttgactttgg ccgaatcgag aagattgtgg gcgcgacggg gcaagtgcag gacagcgtgc
    17701 ttgtcatacg ctttccgcgc agcgacctca aggtgacgat tgacggcgag ccgatgccga
    17761 cggccttagg cttcgtttcg tggacggcgt ggaagcgcat gggcaatgcg gccactgtca
    17821 tgggcgacct ggtgcttctg gagagggaga tcaatcccgt catctccgcc ccggccgaag
    17881 ccaatattga ggtgacggcg ctgcacaacc attttctcgg ggaagagccg cgcatcctgt
    17941 tcatgcacat tcacggcatg ggagatgctg acacgctggc gcgcgggatc catagtgcgc
    18001 tgagcaagac ggcaactccg gcaccagttt ccggtgctac gtcaaccccc ccaccggctc
    18061 tggacaccaa aaagatcgaa gagatcatcg gccatccggg tcaagccagc ggcggcgtat
    18121 tcaaaatcac aataggtcgc cccggcgtca tcgcggacgg gattgaggtg acttccacga
    18181 tggggttgaa cagctgggcg ggatttgtgg gcacgaatga acgcgcgcac gtggccggtg
    18241 acgtggccat gaccaccggt gaagtgaatc gtgtcatccg cgcgctgcgc agcggcggca
    18301 ttgacgtggt ggccgtgca
//
LOCUS       DBNGZA010000329         3248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_167169, whole genome shotgun sequence.
ACCESSION   DBNGZA010000329 DBNGZA010000000
VERSION     DBNGZA010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_167169"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16FU_07240"
     CDS             complement(<1..403)
                     /locus_tag="AC16FU_07240"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR2994199.1"
                     /translation="MTADPPEMVLPPTRPSGKDPLEWLVVLATAVFVGLIYVRSICPT
                     VFWWDSGELIANIKILGIAHRPGFPLYLLLGKVFSFLPFFSFAARINLFSALCATAAA
                     VFLFLTLRRLFGWLCRPAHLAPAFFHLVTCGA"
     gene            complement(400..1761)
                     /locus_tag="AC16FU_07245"
     CDS             complement(400..1761)
                     /locus_tag="AC16FU_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR2994200.1"
                     /translation="MASPKVLVVDDETSLCQFMQIMLAKEGYEVTALSSGQEALSRLA
                     QKRFDVVIADLMMPEMNGLELLSKVREIDPSLAFVVMTAFASVDSAIEALKKGAADYI
                     TKPFKVDEIKIALARLLEQKRLSSENLSLKRQLKKESDFTHFIGAAPAIVAMKKMAEQ
                     VAATDATVLIHGESGTGKEIVARAIHQLSPRADKPFVTINCAALPETLLESELFGHTR
                     GAFTGAVKDKEGLFTVADGGTFFLDEVGETTPAIQVKLLRALEEKEITPVGATKPIQV
                     DVRLIAATNADLEADVKNGKFRTDLFYRLNVFAIAIPPLRERQEDIRLLAEYFVKQAS
                     DKQSRPPKEIAAGTWEVLERYPWPGNVRELENAMERAVLLAKGKAIQPADLPENIRNQ
                     APASIVSPTVPATPTLEEIEKAYIHWVLTQTDWQKSKAAQLLGIDASTLYRKIERYRL
                     SQS"
     gene            complement(1946..>3248)
                     /locus_tag="AC16FU_07250"
     CDS             complement(1946..>3248)
                     /locus_tag="AC16FU_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR2994201.1"
                     /translation="GMRREPLFEFMEPPAEQNYRKFLTLRNIALAINSPATIGEIARE
                     ALAQAVAIVGVEAGLVTILDAKGQQVSQAIAGGEEKKRLLLELESELLISLRKNFGVR
                     SVYLTLDRDGTHTLFSYPLRSNGEVVGAISGVVSGTRNLALEEEFIEALGHQLGIAVG
                     KKVILPTLVKTERPAIVQEFLDYSLDLKQVLTRLHEKLSPRVKYDLVGLVHPRDAVWV
                     DLYLLRRGAGEVLEGRLERIEKTLLEQVASRRTPVLTNRVGETGLHAQVRGLQYVGLV
                     PLVFENDVVGVVYLGAAGMLTQPDLEELRALALPLALAVKNALLYGELRAAYQTLSER
                     QKELLKDERKTAVLEAAVTISHEVNNPLTAVLGNTQLLLLKKEQFPPEVQEKLKVIEE
                     SALRIREVTQKLMNIEEPVSTEYVEGVKMLDLEKSMRRKKE"
BASE COUNT          645 a          948 c          957 g          698 t
ORIGIN      
        1 ccgccccgca agtcacgagg tggaagaagg ccggcgccaa atgggcggga cggcagagcc
       61 agccgaagag acggcgcagg gtcagaaaga gaaagaccgc cgcagccgtg gcgcagaggg
      121 ctgaaaagag atttattcgg gcggcaaagc taaaaaaagg cagaaaggaa aaaacttttc
      181 ccagaagcag gtacaaaggg aaccccggac gatgggcgat ccctaagatt ttgatgttgg
      241 caatgagttc acccgagtcc caccagaaga cggtggggca aatgctccgc acgtagatca
      301 agccgacaaa gacggcggtg gccaaaacca cgagccattc cagcggatct ttccccgacg
      361 gccgggtggg gggcaaaacc atctccggag ggtcggcggt catgactggg acagccggta
      421 gcgttcgatt ttccggtaga gggtggaggc gtcgataccc agaagctggg cggcttttga
      481 cttttgccag tcggtctggg tgaggaccca gtggatgtag gccttttcaa tttcctctaa
      541 ggtcggcgtc gccggcacgg tgggggaaac gatggaggcc ggcgcctggt tccggatgtt
      601 ctccggcagg tcggccggct ggatcgcttt gcctttggcc agaaggaccg cccgctccat
      661 ggcattttcc agttcccgga cgttccccgg ccaggggtaa cgctccagca cctcccaggt
      721 tccggcggca atttccttgg gcggacggct ctgtttgtcg ctggcctgtt tgacgaagta
      781 ttcggccaga agtcggatat cctcctggcg ctcgcgcaaa ggaggaatgg cgatggcaaa
      841 gacgttgagc cggtagaaga ggtcggtccg gaacttgccg ttttttacgt cggcttcgag
      901 gtccgcattg gtggcggcga tcagacggac gtcgacctgg atcggcttgg tggctcccac
      961 cggcgtgatc tctttctcct ctaaggcccg caggagtttg acctgaatcg ccggagtggt
     1021 ctcccccact tcgtccaaga agaaggtgcc gccgtcggcc acggtgaaga ggccctcttt
     1081 atctttgacc gcgccggtga atgcgccccg ggtgtggccg aagagttcgc tttccagaag
     1141 cgtctcgggc agagccgcac agttgatggt gacgaagggc ttgtcggcgc ggggcgagag
     1201 ctggtgaatg gcccgggcca ctatttcttt cccggtgccg ctctctccat gaatcaagac
     1261 ggtggcatcg gtggcggcca cctgttccgc catctttttc atggcgacga tcgccggtgc
     1321 ggccccgatg aaatgggtaa agtcggactc tttcttcaac tggcgcttga ggcttaagtt
     1381 ttcgctggag agccgcttct gctccaggag gcgcgccagg gcgatcttga tttcatccac
     1441 tttgaacggc ttggtgatgt agtcggcggc ccccttcttc aaagcctcga tggcgctgtc
     1501 caccgaggca aaggcggtca tcacgacaaa ggccagggag ggatcgattt ccctcacttt
     1561 tgacaacaac tctaagccgt tcatctccgg catcatcagg tcggcgatga ccacatcgaa
     1621 gcgtttctgc gccaggcggg agagcgcttc ctgcccgctg gaaagggcgg tcacctcgta
     1681 cccctccttg gccagcataa tctgcatgaa ctggcacaga gaggtttcgt cgtccaccac
     1741 cagaactttg ggggatgcca tactattgga gttatcggca gacgggagac cggctacagg
     1801 cgcgtgggcg agaagccgcg agaacaaaag tggagtaaac ctttgtgccg tcttatctcc
     1861 gggaagtgct accgaaaaaa aatcgccttt caacgcgatt tttgctttcc cggtgctccg
     1921 tatactgtgt tcggtgttct gagtttcact ccttttttct ccgcatagat ttctccagat
     1981 ccagcatctt caccccttcc acgtactcgg tcgagaccgg ctcttcgata ttcatcaact
     2041 tctgggtcac ttcccggatc ctgagggcgc tctcttcaat cactttgagt ttctcctgaa
     2101 cctcgggagg gaattgctct ttcttgagca aaagcaactg cgtgttgccc aggaccgcgg
     2161 tgagcggatt attcacttcg tggctgatgg tgaccgccgc ctcgagcacc gccgtcttcc
     2221 gttcgtcctt tagtaattct ttttgtcttt cgctcaacgt ctggtaagca gcacgcagtt
     2281 caccgtagag gagcgcattc ttcacggcca aggcaagtgg gagcgcaagc gcccgcagtt
     2341 cttcaagatc cggctgggtg agcattcccg cggcaccgag gtagaccacg ccgacgacgt
     2401 cattttcaaa gaccaagggc acgaggccga cgtactgcaa tccccgcacc tgcgcgtgca
     2461 gaccggtttc gccgacgcgg ttggtcaaca ccggtgtgcg ccgcgaggcc acttgctcaa
     2521 ggagcgtctt ttcgattcgc tccagccgcc cttccaggac ttctcctgcc cccctgcgca
     2581 agaggtacaa atcaacccag acggcgtcac gggggtggac caaaccgacg agatcgtact
     2641 tcacccgcgg cgacagtttc tcgtgcaggc gggtcaatac ctgttttaag tcaagactgt
     2701 aatcgaggaa ctcctggaca attgccgggc gctcggtttt caccagcgtg gggagaatga
     2761 cctttttgcc gaccgcgatg cctaactggt gccccaaggc ctcgatgaat tcttcttcta
     2821 acgccagatt gcgcgtgccc gagacgaccc ccgaaatagc gccgaccact tcgccgttgg
     2881 aacgcaaagg ataggaaaac agcgtgtgcg tcccgtcacg gtccaaagtc aaatagacgc
     2941 tgcgcacacc gaaatttttc cggagggaga tgaggagttc tgactccagt tccagcaata
     3001 accgtttttt ctcctcccct cctgcaatgg cctgagagac ctgctgccct ttcgcatcca
     3061 gaatggtcac cagcccggct tcgaccccga cgatggcgac cgcctgcgcc agggcctccc
     3121 gggcgatctc tccgatcgtc gccggcgagt tgatcgctaa ggcgatgttc cgcagggtca
     3181 ggaacttgcg gtagttctgc tcggccggtg gctccataaa ctcaaatagc ggctctcttc
     3241 tcattcct
//
LOCUS       DBNGZA010000330         7657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2607556, whole genome shotgun sequence.
ACCESSION   DBNGZA010000330 DBNGZA010000000
VERSION     DBNGZA010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2607556"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC16FU_07255"
     CDS             <1..936
                     /locus_tag="AC16FU_07255"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR2994202.1"
                     /translation="VKCRVYPIGAATKQLESKQLTEIGDLVAAGAVAVSDDGRPIADS
                     RLMRLALEYCKMFDIPVINHCEDPALVGDGVMNESFTSTLLGLRGVPSAAEEVMVARD
                     LKLAEYIGGRVHIAHVSTAGAVELIRQAKAKGIQVTAETTPHHFCLTDELIKEKFDTA
                     LRVNPPLRTKRDVEAIREGLADGTIDIIVTDHAPHAVEEKEVEFDQAPPGMIGLETAL
                     GLVLTELVKPGYLSLSQALAKMTYLPAEILKLKAGKLAPGALADITLFDPDATWTVKA
                     ESFRSKSKNSPFIGRQLTGKVRWTLVGGRVVYYVE"
     gene            complement(1281..1772)
                     /locus_tag="AC16FU_07260"
     CDS             complement(1281..1772)
                     /locus_tag="AC16FU_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994203.1"
                     /translation="MAYDNNIKEQLDRIEKKLGQATPHPHFFGYEYKSKMKIFGMPLV
                     HIAQGVDPQTGKPRVAKGFIAIGNIAVGVFALGGISLGVFSFAGVGLGIVTFAGCAIG
                     LGIAIGGLAVGTIAAGGLAVGYYALGGAAFGAHTISGSGADPQAVEFFKRFWPGIEKI
                     YNR"
     gene            complement(2056..3066)
                     /locus_tag="AC16FU_07265"
     CDS             complement(2056..3066)
                     /locus_tag="AC16FU_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994204.1"
                     /translation="MTLRWAFVRLGLGAALLLTSLRAAARVPEETAPATAREQEVFDE
                     YIRRMQDNLFRVPLAVTEVLKSYTDHRGRGLELPPIDCRGFLGHKVDRLTVRITTDLR
                     HWQPAYVHRKLSDRTQALYSWQVETEVLGKEKRARANNFIMFDPDAFEQSEREFGPFG
                     VLTNLTAIYHELLHGQLQINAILSDRSWQERACACDFPTEESDADHRFIPAMEKSFLV
                     SLAKVNGFAIYEADGVYAADEDGNFSIIVRDDLLRAKLVNSRQATDSWRWITWDPVNV
                     GRVDVSIEATPRGRYTNDIILRGKLEDPQKPGRFSLYVDPPAVLFLQCYTVEPKRRNA
                     GR"
     gene            complement(3162..3668)
                     /locus_tag="AC16FU_07270"
     CDS             complement(3162..3668)
                     /locus_tag="AC16FU_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2994205.1"
                     /translation="MSASLATLVIENFRLRLGTDLPKQIRTSLGELTDEQIWWRPNES
                     SNSVGNLVLHLCGSLRHFLGRGVGGSDYQRDRPKEFSEREPIPKAALLRTVDETFAEA
                     QAILGRLTPERLTEMTDRAGAPTPVYELVLRMAIHVAMHTGQILYITKMLKEGVFPEM
                     IWSWSNTR"
     gene            complement(3704..3997)
                     /locus_tag="AC16FU_07275"
     CDS             complement(3704..3997)
                     /locus_tag="AC16FU_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR2994206.1"
                     /translation="MATFIGLVSFTDQGVRNVKDTTKRAETVKQMAAKAGVKLKDIYW
                     TLGQYDLVSIWEAPDAETLLPLLFALGAQGNVRTQTLRAFNAEEVGKVISRMP"
     gene            complement(4070..4420)
                     /locus_tag="AC16FU_07280"
     CDS             complement(4070..4420)
                     /locus_tag="AC16FU_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR2994207.1"
                     /translation="MPNPVVHWEIAVKNFDKAKAFYSGLFGWKVDSNNPMNYGIVDTA
                     AGGINGGIFQAQKNMPQNYVTFYIQVDDLQKYLTKAESLGGKTCVPPTPIPNMGAFAM
                     FNDPEGNMIGLFKG"
     gene            complement(4433..4894)
                     /locus_tag="AC16FU_07285"
     CDS             complement(4433..4894)
                     /locus_tag="AC16FU_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994208.1"
                     /translation="MKQKSNGQQVEFIGTKLLEAELVRNGFEVAYPIRDRGIDFIIYQ
                     DNHGKPFSAVPIQVKAASGERFILDRKYERMKGLILAYVWGIDKKPRYFLLTYREALS
                     LLGRARKTNSWQKKGIYVYPHVPHRLKEKLHQYYEDRWTWLRKLLRTKTQK"
     gene            complement(4916..6031)
                     /gene="ispG"
                     /locus_tag="AC16FU_07290"
                     /gene_synonym="gcpE"
     CDS             complement(4916..6031)
                     /gene="ispG"
                     /locus_tag="AC16FU_07290"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR2994209.1"
                     /translation="MDSPKKTNGNLPRSRTRQVTVGKIKLGGGAPVVVQSMCTTDTRD
                     VQATLSQIRQLAEADCELVRLAVLDEKAALALKELVPHAPVPIIADIHFNYKLALAAV
                     EAGVQKLRLNPGNVGEDWKVREVVKRVKERGIPIRIGANSGSLPKDIIEKYGKPTPEG
                     IVEAALRHVAILEEMDFHDIVVSLKSSEVRITVDAYRLMAETAPQYPLHIGITEAGTA
                     WAGTIKSSVGLGILLADGIGDTMRISLAADPVEEVKVAYEILKDLEIREHGVTLIACP
                     TCGRCEIDFMPLVQKVEVELRKIREPIRVSVMGCVVNGPGESREADVGVSAGRGLGLI
                     MRKDKVVRKVKEEEIFNALMEEVQKVLAERRTQAVAT"
     gene            6142..6510
                     /locus_tag="AC16FU_07295"
     CDS             6142..6510
                     /locus_tag="AC16FU_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994210.1"
                     /translation="MSPSTHKILLVLFFAILFVAILQLRDTPRQTRLSENEFIALTAD
                     LAVAQSRYSTNPGLWQKEIENILARSRVTREQIETFRQKVDRSPEKWAHIWNEVARKL
                     QEKETAAGHPKTASPPVNPP"
     gene            complement(6497..6862)
                     /locus_tag="AC16FU_07300"
     CDS             complement(6497..6862)
                     /locus_tag="AC16FU_07300"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR2994211.1"
                     /translation="MKKSKTQFGAEVEQTVAQYLTNCGWQILERNWRSGHREIDLIAR
                     DGRALVFVEVKGARSLSFGQPEERVDFAKRRHLNDAALAYLQAHEPEASEYRWDVVTV
                     TFAKGAPTINHLRNAFTAD"
     gene            complement(6904..7575)
                     /locus_tag="AC16FU_07305"
     CDS             complement(6904..7575)
                     /locus_tag="AC16FU_07305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR2994212.1"
                     /translation="MLQGETPGEIPCSGGVDLFAFERVLWESGWELVAGVDEAGRGPL
                     AGPVVACAVVFPKDCPSIPVNDSKKLSAAERLALIGPIGRTALSVGVAVVQQEAIDRF
                     NILQATFSAMRQALTRLRSKPDLVIVDGRQRIPGLAFPQFPLVKGDARSASVAAASIV
                     AKVVRDRIMEEMDETYPAYGFARHKGYATPSHRAALLRFGPSPVHRASFLKKLFAPVQ
                     ERLAL"
BASE COUNT         1572 a         2336 c         2135 g         1614 t
ORIGIN      
        1 gtcaaatgcc gcgtctatcc tatcggggcc gcaaccaagc agttggaaag taaacagttg
       61 accgaaatcg gcgatttggt ggcggcagga gccgtggcgg tctccgacga cggccgcccg
      121 attgccgaca gccgcctgat gcggctggcc ctcgaatact gcaagatgtt cgacatcccg
      181 gtgatcaacc actgcgagga cccggcttta gtcggcgacg gtgtgatgaa cgaaagtttc
      241 acttcgacat tgctcggcct gcgcggggtg ccctcagcgg ctgaagaagt gatggtcgcg
      301 cgtgatctga aattggccga gtacatcggc ggacgggtgc acattgctca cgtttccact
      361 gccggcgcgg tggagcttat ccgccaggcc aaagccaagg gcatccaggt caccgccgaa
      421 acgaccccgc accacttttg cctgaccgac gaactgatca aggaaaaatt cgatacggct
      481 ctgcgggtta atccccccct gcgcaccaaa agggacgtgg aggccatccg tgagggtctg
      541 gccgacggga cgattgacat catcgtcacc gatcacgccc cgcacgcggt ggaagagaag
      601 gaagtggagt tcgatcaggc gccgccgggg atgatcggcc tggaaaccgc gctgggcctg
      661 gtcctcaccg aattagtcaa acccggctac ctatccctct cgcaagctct ggcgaaaatg
      721 acttacctgc ccgcagaaat actgaaattg aaggccggca aactcgctcc gggcgctctg
      781 gctgacatta ccctcttcga ccctgacgca acctggacgg taaaagccga aagcttccgc
      841 tcaaagtcga agaactcccc tttcatcggg cggcaattga ccggcaaggt gcgctggaca
      901 ttggtgggtg gccgggtcgt gtattacgtg gaataataga cccctcccta tccctccccc
      961 acaggggagg gaaggcaccc ttccgaattc gagttaatca ttctcgcgta aagttccctc
     1021 tcctcttagg agagggtcag ggtgaggtca gaacaggatt tgtagccgag gttttcaacc
     1081 tcggcagccc gaagggctgt cgccttttct aatgccacgc aaccctgtgg ggtcgcgccg
     1141 aggatagaat cccctgcagg ttgtaccgac ctcggctatg ccaaaaaatg gcgccggttt
     1201 caaaaacctc ccggcgccac atactgtaac aatgagacat gcaggggtga atccgcctca
     1261 ggcggattca cccagtattt ctatcggttg tagattttct ctattcccgg ccaaaatctc
     1321 ttgaagaatt cgactgcctg cggatcggct ccagacccgg agatcgtgtg ggcgccgaag
     1381 gccgcgccgc ccaaggcgta gtaccccacc gccaagccgc cagcagctat cgtcccaaca
     1441 gccagcccgc caatggcgat acccagcccg atggcacagc ccgcgaaggt cacgatacca
     1501 agccccactc ccgcgaacga gaaaacgccc agacttattc cgccgagcgc aaagacccca
     1561 acggcgatgt tgccgatggc gataaacccc ttggccacgc gcggcttgcc ggtctgggga
     1621 tcgactccct gggcaatgtg caccagcggc atgccaaaaa ttttcatttt cgatttgtat
     1681 tcatagccga aaaaatgggg atgcggcgtt gcctgtccca actttttctc aatgcggtcg
     1741 agctgctctt tgatgttgtt atcgtaggcc atgcgacctc ctctgcttgt taatatcggc
     1801 taatgtatgc ccggcggcca gcggcccgga gtagtaagag acatggggga tcggtgccat
     1861 accgaccttt agttgcaaga ggagcaaaag gtttgggcgt ccgagagtcg atccgacccg
     1921 ttccgagtgt acttatagtg tgggagggat ggttttcttg caccgagcga agtcgaggtg
     1981 cgtatcccgc accttcaggt tacgaaaacc cttcgactcc gctcagggct atccaacctg
     2041 cactacgggc gcatttcacc gaccggcatt ccgccgcttc ggttcgactg tgtagcactg
     2101 caggaagagc accgccggtg gatcgacgta cagactgaac cggcccggct tctgcgggtc
     2161 ctctaatttc cctctcagga tgatatcgtt cgtgtaccgc ccccgcgggg tcgcctcgat
     2221 ggaaacgtcg acccgcccaa cattcaccgg gtcccaggta atccagcgcc aggagtcggt
     2281 ggcctgccgg gagtttacga gtttcgcccg caggaggtcg tcgcgcacga tgatggagaa
     2341 attcccgtcc tcatcggcgg cgtaaacgcc atcggcctcg taaatcgcaa agccgttcac
     2401 cttggccaga cttaccagaa acgatttctc catggccgga atgaaccggt ggtcggcgtc
     2461 ggactcttcg gtgggaaaat cgcaggcgca ggcccgctcc tgccacgacc gatcggagag
     2521 gatggcgttg atctgcaact gcccgtgtaa cagttcgtgg tagatggcgg tgaggttggt
     2581 caaaacaccg aaggggccaa actctctctc tgactgttca aacgcatccg gatcgaacat
     2641 gatgaaattg ttcgcccgcg cgcgcttttc cttccccagg acctccgtct ccacctgcca
     2701 agagtagagt gcctgcgtcc ggtcgctcag tttccggtga acgtaggcgg gctgccagtg
     2761 cctgaggtcg gtggtgatgc ggaccgtgag gcggtcgacc ttatggccga gaaatccccg
     2821 gcaatcgatc ggcggaagct ccaggccccg tccccggtgg tcggtgtaac ttttgagcac
     2881 ttccgtaacc gccaaaggca cccggaagag attgtcctgc atgcggcgga tgtactcatc
     2941 gaacacctct tgctcccggg cggtagctgg agcggtttct tcaggaacgc gtgccgccgc
     3001 cctgaggctc gtcaggagca gggcggcacc cagtcccagg cgcacgaaag cccaacggag
     3061 agtcatcttt ccttacatcg gaagaatcaa gccgaattgt tacaggggaa aagccctgcg
     3121 agacaggtct tgccgccgcg aggcgcaccg gggctgttcc cctaccgggt attgctccac
     3181 gaccagatca tctcggggaa gaccccttct ttgagcatct tggtgatgta gaggatctgg
     3241 ccggtgtgca tggccacgtg aatggccatc cgcaggacta actcgtagac cggcgtgggg
     3301 gcgcccgccc ggtcggtcat ttcggtcagg cgctcggggg tcaagcggcc cagaatcgcc
     3361 tgtgcttcgg cgaaggtttc atctaccgtg cgcaagagcg ccgctttcgg gatcggctcc
     3421 cgttcggaga attctttggg ccggtcgcgc tggtagtcgg agccgcccac gccacggccc
     3481 aggaaatgcc gcagcgatcc gcagaggtgc aggaccaagt tgccgaccga gttgctcgac
     3541 tcattcggcc gccaccagat ttgctcgtcg gtcaattccc ctaaagaggt acgaatctgc
     3601 ttggggagat ccgttcccag ccgcaggcgg aaattttcta tcactagggt cgccagtgag
     3661 gcactcatcg actgatccga tgctttgctt ggaatcgcgc ggcctacggc atccggctga
     3721 ttactttgcc gacctcctcg gcattaaaag cccgcagagt ttgagtgcgg acattgccct
     3781 gtgcacccag ggcgaacaga agcggcagaa gcgtttcggc gtccggcgct tcccagatcg
     3841 acacgaggtc gtactggccc agggtccagt agatatcctt taacttgacc ccggccttag
     3901 ccgccatctg cttgaccgtt tccgcccgct tggtggtgtc tttgacgttc cgaactccct
     3961 ggtcggtgaa actcaccaga ccgatgaaag ttgccatgac cgacctcctc taataatgag
     4021 caacagttta cgttaccgtc taccagctac tcgctcgctg gaaattgttc taccctttga
     4081 acaagccaat catattccct tcgggatcgt tgaacatagc gaaagcgccc atgttcggaa
     4141 tcggcgtggg cggcacgcag gttttgccgc ccagactttc ggccttggtc aggtatttct
     4201 gcaggtcatc cacctggatg tagaaggtga cgtaattctg gggcatgttc ttctgggcct
     4261 ggaaaatccc gccgttgata ccgcccgcgg cggtatcgac tatgccgtag ttcatcgggt
     4321 tgttgctgtc gactttccag ccgaagagcc cggagtagaa ggccttggct ttgtcgaagt
     4381 tcttgacggc gatttcccag tggactaccg gattgggcat tgcatgctcc tttcattttt
     4441 gtgtttttgt gcggagtaac ttccgcaacc atgtccatcg gtcttcataa tattgatgta
     4501 atttctcctt cagccggtgg ggaacgtggg ggtatacata aattcccttt ttctgccatg
     4561 aattggtttt tctggcacga ccgagaagtg agagagcctc acgataagtc agcagaaagt
     4621 aacgtggttt tttgtcgatg ccccagacgt atgccaagat caagcccttc atgcgttcat
     4681 atttccggtc gagaataaac cgttcgcccg aggccgcctt tacctgaatg ggaaccgccg
     4741 aaaaaggttt cccatgattg tcctgataaa ttatgaaatc aattcctcgg tcgcgaatcg
     4801 ggtatgccac ttcaaaaccg ttgcgcacca gttccgcttc caaaagtttt gtgccgatga
     4861 actccacttg ctgtccattt gacttttgct tcaccaacgt atcctcagac gacctctatg
     4921 tggcaaccgc ctgcgtccgc cgctccgcca gcactttctg cacttcctcc atcagggcgt
     4981 tgaagatttc ctcttctttc accttgcgca ccactttgtc cttgcgcatg atgagcccca
     5041 ggccccggcc agcggagacc cccacgtcgg cttcgcgcga ttcgccgggc ccgttcacca
     5101 cgcaccccat caccgagacc cgtatgggct ctctgatctt gcgcagttcg acttcgactt
     5161 tctgaactaa cggcataaag tcgatttcgc accggccgca ggtggggcag gcgatgagcg
     5221 tgacgccgtg ctcgcggatt tccaggtctt ttaagatttc gtaggcgact ttgacctctt
     5281 ccaccggatc agcggcgagc gaaatccgca tggtgtcgcc aatgccgtcg gccaaaagaa
     5341 tgcccagacc gacggaagac ttaatcgtcc cggcccaggc ggtgccagct tcggtgatgc
     5401 ctatgtgcag gggatactgc ggcgcagtct cggccatcag gcggtaggca tcgaccgtta
     5461 tgcgcacttc cgacgacttg agcgaaacga cgatgtcatg gaaatccatt tcctccaaga
     5521 tagcaacatg gcgcaaagcg gcttccacaa tcccctccgg cgtcggctta ccgtattttt
     5581 cgataatgtc tttgggcaaa gatccggagt tagcaccgat gcggatggga ataccccgtt
     5641 ccttcacccg cttgaccacc tcccgcacct tccagtcttc gccgacgttg ccgggattga
     5701 gccgcagttt ctgcactccg gcctccacgg ccgccaaggc caacttatag ttgaagtgga
     5761 tgtcggcgat gatcggcacc ggcgcgtgcg ggacgagctc ctttaaggcc aaggcggctt
     5821 tttcgtccaa aaccgccagg cgaactaatt cgcagtcggc ttcggccaac tggcggatct
     5881 ggctcaaggt ggcctggaca tcccgcgtat cggtggtgca catcgactgc accaccaccg
     5941 gcgcgccccc gcccagtttg atttttccca ccgtcacctg ccgggtacgg ctgcgcggca
     6001 gattgccgtt ggtcttcttg ggactatcca ttctttcctt catagtaagg tgtcaacagg
     6061 ggcttcacaa caatgcccga aacaatctgc cttgcgctga cgccttcttg acttccaccg
     6121 cgacaatcct actttcgctt tgtgtcgccg tctacccaca agatactact tgtcctcttt
     6181 ttcgccatac tgttcgtggc catcctccag ctccgcgaca ccccgcgcca gacccggttg
     6241 agtgaaaacg aattcatcgc cctgacggct gacctggccg tcgcccaaag ccgctacagc
     6301 accaatcccg gactctggca gaaagaaatt gaaaatatcc tggcgcgctc ccgcgtcacc
     6361 cgcgaacaaa tcgaaacatt ccgtcaaaaa gtcgaccgct ctccggaaaa gtgggcgcac
     6421 atctggaacg aggttgcccg aaaacttcag gaaaaagaga ccgccgcagg acacccgaaa
     6481 actgcctcac cgccggtcaa tccgccgtaa aggcgttgcg caggtggttg atggtgggcg
     6541 cgcctttggc aaaggtcacc gtcactacgt cccagcggta ttcggaggcc tccggctcgt
     6601 gcgcctgaag ataggccaaa gccgcgtcat ttaagtggcg gcgtttggcg aaatcgaccc
     6661 gctcttccgg ctggccgaac gaaagcgacc tcgctccttt cacttccaca aagactaaag
     6721 cacgcccgtc ccgggcgatt aaatcaattt cccggtggcc cgagcgccag ttacgctcca
     6781 gaatctgcca gccgcaattg gtgaggtact gcgcgacggt ctgttcaact tcggcgccga
     6841 attgagtttt tgacttcttc actatcctga tatggataaa gaaacgaccg aacgccttcc
     6901 gacttacagt gccagccgct cctgcaccgg cgcgaacagt tttttcaaaa aactggcccg
     6961 gtgcaccggc gacggtccga atctcaaaag cgccgcgcgg tgcgacgggg tggcatagcc
     7021 tttgtgccgg gcaaaaccat aggcgggata cgtttcgtcc atttcctcca taatccggtc
     7081 gcggacgact ttggccacga tggaagcggc ggcgaccgac gccgaacggg cgtcgccttt
     7141 cactaagggg aactgcggga acgccaggcc ggggatgcgc tgccggccat ccacgatgac
     7201 taagtcaggc ttagagcgca aacgggtcag cgcctgccgc atggcactga aggtggcctg
     7261 gaggatattg aagcggtcga tggcctcctg ctgcaccacc gccacgccga cggaaagggc
     7321 tgtccggcca atcggcccga tgagcgccag acgctcggcg gcggaaagtt ttttggaatc
     7381 gttcacgggg atggaaggac aatcctttgg aaataccact gcgcaggcca ccaccggacc
     7441 ggccagaggc ccccggccgg cttcatccac cccagccacc agctcccagc cgctctccca
     7501 gagtacccgc tcaaaagcga aaagatcaac cccgccagag catggaatct cgcccggtgt
     7561 ttcaccctgt agcacgaacc ggttgagctc gggaattcgg gtcttcgtcc gacgcacggc
     7621 gggttaagtc aggcaactgc ggcttcggct cttgccc
//
LOCUS       DBNGZA010000331         1383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1123598, whole genome shotgun sequence.
ACCESSION   DBNGZA010000331 DBNGZA010000000
VERSION     DBNGZA010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1123598"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="AC16FU_07310"
     CDS             <1..968
                     /locus_tag="AC16FU_07310"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2994213.1"
                     /translation="TSLNEPFTFVGTATGGMELFTDNTAQNNIDYYYKIVAVAGDKSQ
                     AESLPSAPAHAYQQWFNAKRVNLLIGVLLLSLAIVYFIEQAKKGKPLFIRKIAGLEAV
                     DEAVGRATEMGKKIIYIPGIQDLDNVQTVAGVTILGRVAKVAAEYETHLEVPTSRSLV
                     MVACREAVQEGYSLAGRPDAYNQDRIYYLTDEQFGYAAGVDGIIVREKPAAIFYQGCF
                     FAESLILSETGNSIGAIQIAGTAQPTQLPFFFAACDYTLIGEELFAASAYLSREPRQL
                     GSLKGQDIGKALIMLAILAGALWQTLAVFFPETFHSNAASLFLTE"
     gene            975..>1383
                     /locus_tag="AC16FU_07315"
     CDS             975..>1383
                     /locus_tag="AC16FU_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994214.1"
                     /translation="MRREIPLILTAIVGLTLVIQFFIPHEPFNKMQTWFADWVAIIQA
                     FAIWLGALNLLVVSAEKVSKKQPGWFYSVVIIASFFLIVIPGLFFSGGRHFQEAGTSF
                     DWLYVHMFTPLSATMFALLAFFVASASYRAFRAR"
BASE COUNT          263 a          429 c          376 g          315 t
ORIGIN      
        1 gcaccagctt gaacgagccg ttcacgttcg tcggcaccgc caccggcggg atggaactct
       61 tcaccgacaa cacggcccaa aacaacatcg attactacta caaaatcgtt gcggtggcgg
      121 gcgacaaaag ccaggccgaa tcactcccca gcgctccggc gcacgcctac cagcagtggt
      181 tcaacgccaa gcgggtcaat cttttgatcg gcgtactgct tctgtctttg gccatcgtct
      241 acttcatcga gcaagccaag aagggcaagc cgcttttcat ccgcaagatc gccggtctgg
      301 aggcggtcga tgaagccgtc gggcgcgcca ccgaaatggg caagaagatc atttatatcc
      361 ccgggattca agacttggat aacgtccaga ccgtcgccgg agtaacaatt ctggggcgcg
      421 ttgcgaaagt ggcggccgag tatgagacgc atttggaagt accgacgtcc cgctctctcg
      481 tgatggtggc ctgccgggag gcggtgcagg aggggtactc cttggccggc cggcccgacg
      541 cttacaatca ggaccggatt tattacctga ccgacgagca gttcggctac gccgccggcg
      601 tggacgggat catcgtacgc gaaaaaccgg cggcgatctt ctaccagggc tgcttttttg
      661 ccgagtcttt gattctttcc gaaacgggta attccatcgg cgccattcaa atagcgggta
      721 cggcccagcc gacgcagctg ccctttttct tcgccgcctg cgactacacg ctcattggcg
      781 aggaactttt cgccgcctcc gcctacctct cgcgggagcc gcggcagttg gggagcctca
      841 aggggcagga tatcggcaag gcgttgatta tgctcgccat tctggccggg gccctctggc
      901 aaacgttagc ggtcttcttc ccggagacat ttcactccaa cgcggccagt ctgttcctga
      961 ccgaatagaa atcaatgcgg cgcgaaattc ctttaatcct caccgccatc gtcggtctca
     1021 ccttagtgat tcagttcttc atcccgcacg aaccgttcaa caagatgcag acttggttcg
     1081 ccgactgggt ggcgataatc caggccttcg ccatctggct gggagcgctg aaccttttgg
     1141 tcgtctcggc agagaaagtc tccaaaaagc agccgggctg gttctattcg gtcgtcatca
     1201 tcgccagttt ctttttgatc gtcatcccgg gactcttctt ctccggcggg cggcacttcc
     1261 aggaggccgg gacctccttc gactggctct acgtccatat gtttactcct ctctcggcca
     1321 ccatgttcgc tcttttagct tttttcgtcg cttccgcctc ctaccgtgcc tttcgcgccc
     1381 gca
//
LOCUS       DBNGZA010000332         5618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1546879, whole genome shotgun sequence.
ACCESSION   DBNGZA010000332 DBNGZA010000000
VERSION     DBNGZA010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1546879"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2999
                     /locus_tag="AC16FU_07320"
     CDS             <1..2999
                     /locus_tag="AC16FU_07320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR2994215.1"
                     /translation="DVKKAKEKAEEYLKIFGEGNFLIELQDHGLEEEAKIRRALAKVA
                     SDLNVPIVGTNDCHYLKREHAAAHDALLCIQTGKLLTDTDRLKYETDQIYFKSPAEMK
                     ALFADYPDAVEHTLRIAELCNLELELGKIHLPHFPLPHGFESNDQYLEYLARKGLTHR
                     YSHVTPALEERLDYELKIIRQTGYAGYFLIVKDFIDYARHQNIPVGPGRGSAASSIVS
                     YALGITSIDPIKYGLVFERFLNPERISMPDIDIDFADRDREKIIEYVTRKYGQANVSQ
                     IITFGTLAARGVIRDVGRVMALPYSEADRIAKLIPFEIDMTIDRALELEPDLKRLADS
                     DPKVQKLLDFSRTLEGLTRHASTHAAGVVIAPTELTNYVPLYKTNKDEVITQFDMKGI
                     EEIGLLKIDFLGLRTLTVIQETVRLVLEHHGLALDIENIPLDDQKVYDLFGRGDTVGL
                     FQFESSGMRDYLRKLKPTGLEDLAAMNALYRPGPLDSFMVDEFIARKKGEKQIVYEHP
                     LLEPILRETYGVIVYQEQVLRIASELAGYSPGKADILRKAMGKKIAELMQEQRAEFIA
                     GARARGIRKETAEKTFELIATFGRYGFNKAHSVGYALLAYQTGYLKIHYPVEFTAASL
                     TSEMGNTDRIVILMDECRRMGIEVLPPDVNESNATFSVVGNQIRFGLAAVKNVGENAV
                     AAIVEARGKFGRFQTLFQFCERVDLAAINKRALESLIMAGACDSLKGHRAQLLLAIPD
                     ALGYGQLANEERKRGQTSLFAGGAKHTPVPEPRLPDAPEWSLAEILSKEKEMLGFYVS
                     GHPLTKHQDHLRLYATHTSEALATVRDGQEVTVGGIVVAVKTNIDKKGKQMAFATLED
                     FAGTIETVIFADLYEKNKGLVRADQMILLRGRASTKEGEKAKVVAAEINPLDQAAIAI
                     PLSLTLVLNDATVSETALDAIAPLLSQAQGPCPVILCFRHNGEEIRLRSKRYKVKAST
                     ELLQNLQEILGEEGVYFARPNRNSNH"
     gene            complement(3083..3754)
                     /locus_tag="AC16FU_07325"
     CDS             complement(3083..3754)
                     /locus_tag="AC16FU_07325"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994216.1"
                     /translation="MTEITAELQDVTKVYQRGNERVHALAGISLSIEMGDFLAIVGPS
                     GAGKTTLLNLIGCVDSPTSGSIRVNGKETTQLSDRELTAIRKKTVGFVFQQFFLIPTL
                     TARENVLVPTLFLRENGDKSKKARELLDLAGLAHRANHLPSQLSGGEMQRVAIARALI
                     NNPSLLLADEPTGNLDSKQAENIIGILTALNQRGMTVILVTHNLSLAKNCRNLIHLRD
                     GKIIS"
     gene            complement(3747..4907)
                     /locus_tag="AC16FU_07330"
     CDS             complement(3747..4907)
                     /locus_tag="AC16FU_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994217.1"
                     /translation="MTIFSLALKNLKRHRTRTILTILGIAVAAATLFSILSFDAGYKK
                     ALQEELAGSGIHLFVSTEGCPLEAASLIIHGGEIPRFLNMQTFNAVKDIPGVKQAGGF
                     LIFSLPSSDGSKVDLFYGITEDVLRLKPNWKMQGGWFKDENSIILGSEVARVEKRGVG
                     DKIYIESLDKEFTVTGILEKTYGQDDGFYFLPIETAQKLFKKENKLTAVGVQLQDLSQ
                     MGSVKSRLETLPDVYVVPAEQMSQEILKFIGGTKALMYSILFIALVVSTLGLLNTVLM
                     ATFERQKEFGYLRCVGAGRGDVIKLVLIETLIVSLGGASIGFLGGYLFSSGIDQWIRQ
                     FLPYVPAGKLLQPNFAILILAGLIVLFLGVVAGLYPSIRAARVSPMEAIRND"
     gene            complement(4904..5323)
                     /locus_tag="AC16FU_07335"
     CDS             complement(4904..5323)
                     /locus_tag="AC16FU_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994218.1"
                     /translation="MKKIGIISAVGLAVLLGAVSVTAKTKKMGKRIPEKATVVKLSDI
                     LKAPEKFKDKEVILQGTYVNYCCATDFVYREGLESVEIYPQGFLTPKLDKGKQMKVWG
                     IVRVREREEGEQQETRKNAKEEEHAEIIIEAEGLEVK"
     gene            complement(5337..>5618)
                     /locus_tag="AC16FU_07340"
     CDS             complement(5337..>5618)
                     /locus_tag="AC16FU_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994219.1"
                     /translation="ADAEKGEFGVIEVVVGITPEGKVKGVYIQRAREKNKEFKSREFL
                     SQFEGKTVKDSLLIGKGIQVKERSLPVQMTVLGVRKMLIFYDQLANPRR"
BASE COUNT         1291 a         1587 c         1454 g         1286 t
ORIGIN      
        1 acgacgtgaa aaaagccaaa gaaaaagcgg aggaatacct gaaaattttc ggtgaaggga
       61 atttccttat tgaacttcaa gatcacggac tggaggaaga ggcgaaaatt cgccgtgcgc
      121 tggcaaaagt ggccagcgat ctgaatgttc ccatcgtcgg gaccaacgac tgccactacc
      181 tcaagcgcga gcacgccgcc gcccacgacg ccctcctctg cattcagacc ggaaaacttt
      241 tgaccgacac cgaccggctg aagtacgaaa ccgatcagat ttacttcaag tcgcccgccg
      301 agatgaaagc gctctttgcc gactatcccg acgcggtgga gcacaccctg cgcatcgctg
      361 aactgtgcaa cctggagttg gaactgggca aaatccacct gccccatttc cccctcccgc
      421 acggatttga gtcgaacgac cagtaccttg agtatttggc tcgcaaaggg ctgacgcacc
      481 gttattcgca cgtcaccccg gcgctggaag agcggttgga ctatgaattg aaaatcatcc
      541 gccagaccgg ctatgccggc tattttctga tagtcaaaga tttcatcgat tacgcccgcc
      601 accagaacat cccggtggga ccgggccgcg ggtcggcggc ttcgagtata gtttcctatg
      661 cgctggggat taccagtatc gatccgatta agtacggact ggtcttcgag cgctttttga
      721 acccggagcg gatctcgatg cccgacatcg acatcgactt tgccgaccgc gaccgagaga
      781 aaattatcga gtatgtaaca cgcaagtacg gccaggccaa cgtcagccag atcatcacct
      841 tcggcacgct ggccgcccgc ggcgtcatcc gcgacgtggg ccgggtgatg gccctgccgt
      901 attcggaagc ggatcggatt gccaaactga tcccttttga aattgacatg accatcgacc
      961 gggcgctgga actggagccg gatttgaagc gcctggccga ctccgaccct aaagtgcaaa
     1021 aattgctcga cttctcccgg actttggaag gcctgacccg ccacgcctcc acccacgccg
     1081 ccggcgtagt gatcgcgccg acggagctga ccaactacgt cccgctctac aaaaccaata
     1141 aggacgaggt gatcacccag ttcgacatga aaggcatcga ggagatcggc cttttgaaaa
     1201 tcgattttct cggcctgcgg actttgaccg tcattcagga aacggtgcgg ctggtcttgg
     1261 agcaccacgg cttggcgctg gacatcgaaa atattcctct ggacgaccag aaagtctacg
     1321 acctcttcgg ccggggcgac acggtggggc ttttccagtt cgaatcgtcc ggcatgcgcg
     1381 actacctgcg gaagctgaag cccaccggcc tggaagactt ggccgccatg aacgccctct
     1441 accgtccggg ccctttggac tcttttatgg tggacgaatt catcgcccgc aagaaaggcg
     1501 agaagcaaat cgtctacgag caccccctct tggagccgat cttaagagaa acctacggcg
     1561 tcatcgtcta ccaggaacag gtgctacgga ttgcctccga gttggccggc tattctccgg
     1621 gcaaagccga catcctgcgc aaggcaatgg gcaaaaaaat cgccgaacta atgcaggagc
     1681 agcgtgctga gttcattgcc ggcgcccgcg cgcgcgggat taggaaagaa accgccgaaa
     1741 agaccttcga actgatcgcc accttcggac gctacgggtt taacaaggcc cactcggtcg
     1801 gctacgcact tttggcttac caaaccggct acctcaaaat tcactatccg gtggagttca
     1861 cggccgcctc cctcacctcc gaaatgggga acactgaccg gattgtgatc ctgatggacg
     1921 agtgccgccg gatgggaatc gaggttctgc cgcccgacgt caacgaatcg aacgccactt
     1981 tcagcgtggt ggggaaccag atccgcttcg ggctggccgc ggtgaagaat gtcggtgaga
     2041 acgccgtggc cgccatcgtg gaagcccgcg ggaagtttgg ccgtttccag actctttttc
     2101 agttctgcga gcgggtcgat ctggcggcta tcaataaacg tgccctggag agcttgatta
     2161 tggccggtgc ctgcgactcg ctgaaaggtc accgggcgca acttttgctc gccattccgg
     2221 atgcgttggg ttacggtcaa ctggcgaatg aagagcgcaa acgcggccag acctcccttt
     2281 ttgcgggcgg cgccaaacat actccggtac cggagccgcg actgcccgat gcgccggagt
     2341 ggtcgcttgc cgaaattctt tccaaagaaa aagaaatgct gggcttctac gtctccggcc
     2401 atccattaac caagcatcag gaccacctcc gcttgtacgc gacccacacc agcgaagcgc
     2461 tcgcgacggt ccgcgacggg caagaagtta ctgtcggcgg catcgtggtg gcggtgaaaa
     2521 ccaatatcga caagaaaggc aaacagatgg cctttgccac gctggaagac ttcgccggca
     2581 ccatcgagac ggtcatcttt gccgacctgt acgagaagaa caaaggcctg gtgcgggccg
     2641 accagatgat ccttttgcgt ggtcgagcgt ccaccaaaga gggggaaaaa gccaaagtgg
     2701 tggcggctga aatcaacccg ctcgatcaag ccgcgattgc tatcccgctt tcactgacgc
     2761 tggttttgaa tgacgcgacc gtatccgaaa ccgccctgga cgcaatcgca ccgctattat
     2821 ctcaggctca aggtccctgt cctgtgattc tctgcttccg acacaatggg gaggaaatcc
     2881 gcctgcgctc caagcgttac aaagtcaaag cctcaacgga actactgcaa aatttgcagg
     2941 aaattctggg ggaagaaggg gtctacttcg cacgcccgaa tcgtaacagc aatcattgaa
     3001 tctcctcgtc agggagaaga gaatgctggc gtaagcgagt tggagttttg attgtttgaa
     3061 gctgccgcgg attgtttccc aattaagaaa ttatttttcc gtccctcaaa tgaatcaagt
     3121 ttctgcaatt ttttgccagc gacagattgt gcgttaccaa aatcaccgtc atgccccttt
     3181 ggttcagcgc agttaagata ccgatgatgt tttctgcttg tttggagtcc agattgccgg
     3241 tgggctcatc ggccagtaac aaagacgggt tgttgataag agctctggcg atcgccactc
     3301 tttgcatttc tcccccggag agctgggagg ggagatgatt cgctcgatga gcaagtcctg
     3361 ccagatctaa gagttcacgc gcctttttgg acttatcgcc gttctctcga agaaatagcg
     3421 tgggaaccag cacattctct ctggcggtta gggtgggaat taaaaagaac tgttggaata
     3481 caaaccccac cgtttttttc cggattgcgg tcaattcccg gtccgacagt tgggtggttt
     3541 cctttccgtt gacccggata cttcctgaag tagggctgtc aacgcacccg atcaagttga
     3601 gcaacgtggt tttgccagca ccggacggac ccacgatggc taagaaatcc cccatttcaa
     3661 tagagaggct gatccctgcc aaagcatgaa ctcgttcatt gcctctttgg taaaccttgg
     3721 tgacgtcctg aagttcggcg gtaatctcag tcatttctga ttgcctccat gggggagact
     3781 ctggctgccc gaatactggg gtacagccca gctacgacgc ccaggaataa aacgatgaga
     3841 ccggccagga tcaggatggc gaaattgggt tgtaagagtt tcccggctgg cacgtagggg
     3901 aggaattgtc gtatccattg gtcaataccg gaggagaaga gatatccacc gagaaatcca
     3961 atactcgcgc cgcccagaga gacgattaat gtttcgatca ataccaactt gatgacatcg
     4021 cctctgccgg caccgacgca tctcaggtag ccgaactctt tctggcgttc aaaggtagcc
     4081 atgaggacgg tattcagcag ccccaaggtg ctaaccacga gcgcaataaa aaggatggaa
     4141 tacatcaacg ccttcgtccc gccgatgaac ttcagtatct cctggctcat ctgttcagct
     4201 ggcaccacat agacgtcggg taaagtctcc aagcgagatt ttaccgatcc catctgggag
     4261 aggtcctgca attggacccc caccgcagtg agtttgtttt ccttcttaaa taatttttgg
     4321 gcagtctcaa ttggtaaaaa gtagaaccca tcgtcttggc cataggtttt ttcgaggatg
     4381 cccgtgaccg tgaactcctt atccaggctc tcaatgtaaa tcttgtctcc gaccccccgt
     4441 ttttccactc tggcaacttc tgagcctaag atgattgagt tttcatcctt gaaccaacct
     4501 ccctgcatct tccagtttgg tttgagcctc aggacatctt cggtaatgcc gtaaaagagg
     4561 tcaactttcg agccatccga cgaggggagg gagaaaatga gaaacccgcc ggcttgtttc
     4621 acccctggaa tgtctttcac cgcgttgaac gtctgcatgt tcagaaatcg gggtatctcg
     4681 ccgccgtgga tgatgagcga agccgcttcc aaggggcagc cttcggtcga gacaaacagg
     4741 tgaattcccg agccggccag ttcctcctgc agggccttct tataacctgc atcaaacgag
     4801 agaatggaaa agagcgtggc tgcagcgacg gcaatcccca gaatggtcag gatagttctg
     4861 gtcctatgtc tcttgagatt tttcaacgcc agagaaaaga tggtcatttc acctccaggc
     4921 cttcggcttc gataataatc tcggcgtgtt cttcttcctt agcgtttttc ctcgtttcct
     4981 gctgttctcc ctcctccctc tccctgactc gcacaattcc ccagaccttc atttgcttcc
     5041 ctttgtccag cttgggagtg aggaaccctt gcgggtagat ttccaccgac tccagacctt
     5101 cgcggtagac gaagtcggtg gcacagcagt agttgacgta cgtcccttgt aagatcactt
     5161 ccttgtcctt aaacttttcc ggggctttca gaatgtcact cagtttaacg acggtggcct
     5221 tttccgggat gcgcttaccc attttcttcg tcttggccgt taccgaaacc gcaccgagca
     5281 aaaccgcaag cccaactgcc gagatgatac cgattttttt caccgcgacc tccctttcat
     5341 ctgcgggggt tggctaactg gtcataaaag atgagcatct ttcgtactcc caacacggtc
     5401 atttgcaccg ggagcgaccg ttcttttacc tgtatacctt ttccgatcaa aagcgaatct
     5461 ttgacggttt tcccttcgaa ttgacttaaa aattctctgg atttgaattc cttattcttt
     5521 tcccgggctc tctggatgta gacgcctttc actttcccct ctggcgtaat ccccacgacg
     5581 acctcaataa caccgaattc gcccttctcc gcatcagc
//
LOCUS       DBNGZA010000333         3434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2183958, whole genome shotgun sequence.
ACCESSION   DBNGZA010000333 DBNGZA010000000
VERSION     DBNGZA010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2183958"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="AC16FU_07345"
     CDS             complement(<1..753)
                     /locus_tag="AC16FU_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994220.1"
                     /translation="MRNEKLIEIVDLHKAFRNQPVLQGVNLTIHRGETTVIIGRSGCG
                     KSVLLKHLIGLVQPDRGHIYVDGDEITSLSRKRLYALRQKFGMLFQASALFDSLTVAE
                     NVALGLREHTRLSDSEIEEIVREKLRLVGLADVEQLKPAELSGGMRKRVALARAIALD
                     PEIILYDEPTTGLDPIMADVINELVIALRNQLKITALAVTHDIVSAYKIADRVAMLYD
                     GVIIHDGTPEETRQSTNPVVQQFIHGQAEGPIQ"
     gene            complement(761..1537)
                     /locus_tag="AC16FU_07350"
     CDS             complement(761..1537)
                     /locus_tag="AC16FU_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR2994221.1"
                     /translation="MTHALRLVGRQTLVILSDVGGLSVLAGRFVLGLPDFFRRFRLTT
                     NQMLAIGVNSLPLILITSTFVGAVSAWQAAYQFKGYVPLRYLGATVGKAVFIELGPVL
                     TALVVAGRVGASIAAELGTMKVTEQIDALETLAVDPVRYLVVPRIVAGITMLPLLTIF
                     SDAIAIAGALVVSVLFVDLSSETFLNSLKLFFSSSDLFSGLFKAAVFGGIIGLVGCYH
                     GLQAEGGAEGVGRSTTRAVVTASVLILISDYVVATILFSI"
     gene            complement(1645..3018)
                     /gene="dnaB"
                     /locus_tag="AC16FU_07355"
     CDS             complement(1645..3018)
                     /gene="dnaB"
                     /locus_tag="AC16FU_07355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR2994222.1"
                     /translation="MPDDILSGERLPPQAVEVERQVLAAILLDKTALDRVASLITPDA
                     FYREAHRQIYQAMLDLYEKSEPVDLVTVSEALIKSGQLETIGGRAYLASLLEGVATAA
                     HVEHHAKIVLERYTLRTLAQTATEIVSRCFDPTEEVDELLDRAESRIFSIKEAMVRQS
                     ALPLGEILPHTFESIEEYHKRGGNITGLPTGFTDLDVQTAGLQPADFIVVAGRPSMGK
                     TAFILNVAEHVGCDHQIPLVIFSLEMSKNQLAQRMLCSRGRVSSHAMRTGRLRNEEWA
                     NLSIAVGPLSESKIFIDDTPNMDVLELRAKARRLKTQHKIGLVVIDYMQLMQGPRNAE
                     NRQQEIALISRSLKGLAKELDVPVVALSQLSRAVEVRGGKRRPQLADLRESGAIEQDA
                     DVVMFIYRPKVYDIETTEEGLPTENLAEIIVAKQRNGPTGTVRLSFIPEYARFENMAY
                     VGEQAPF"
     gene            complement(3137..>3434)
                     /locus_tag="AC16FU_07360"
     CDS             complement(3137..>3434)
                     /locus_tag="AC16FU_07360"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR2994223.1"
                     /translation="PENREPLPEEIAACFPYLQKQIELIKPKFIVCLGRIAAVTLLNT
                     KLPLGKLRGAFHDYQGIKVLVTYHPAALLRFPSYKKDVWDDMKILRKAYDGTVL"
BASE COUNT          735 a          985 c         1010 g          704 t
ORIGIN      
        1 ctgaatcggt ccctccgcct gaccgtgaat gaactgttgc accactgggt tcgttgactg
       61 ccgcgtctct tccggcgtcc cgtcgtggat gatgactccg tcgtagagca tcgccacccg
      121 gtcggcaatt ttgtaagccg aaacgatgtc gtgggtcacg gccagagcgg tgatcttcaa
      181 ctggttgcgc agcgcgatga cgagctcgtt gatcacatcg gccataatcg gatccaggcc
      241 ggttgtgggt tcatcgtaga gaataatttc cggatcgagc gcaatggccc gcgccagagc
      301 cacccgcttg cgcatccccc cggagagttc cgccggtttg agttgctcca cgtccgcaag
      361 gccaaccagc cgcagtttct cgcggacaat ctcctcgatt tcgctgtccg aaagccgggt
      421 atgttcgcgc aaccccagag cgacattctc cgccacggta agtgagtcga acaaggccga
      481 ggcctggaag agcatgccga atttctgacg cagggcgtag agcctttttc tcgacaggct
      541 cgtgatctcg tcaccgtcga cgtaaatgtg cccccggtcg ggctggacga gcccgatcaa
      601 gtgctttaag agcacgctct tgccgcaacc ggagcgaccg atgatgaccg tcgtctcccc
      661 ccggtggatg gtgaggttca ccccttgcaa gaccggctgg ttgcggaacg ctttgtgcag
      721 gtcaacgatt tcaatcagtt tttcgtttcg catcggtgcg tcagatggaa aagagaatcg
      781 tggcgacgac ataatcggaa attaaaatga gaaccgaagc ggtcaccacc gcgcgggtgg
      841 tggagcggcc cacgccttcg gcgccgcctt cggcctggag cccgtggtag cacccgacca
      901 agccgatgat cccgccgaac acggccgcct tgaacaatcc cgagaacaaa tccgacgagg
      961 agaagaaaag tttgagactg ttgaggaatg tctccgaaga taaatcgacg aagagcaccg
     1021 acacaaccag agccccggca atggcaatgg catcggagaa gatggtcaaa agcgggagca
     1081 tcgtgatccc ggccacgatc cgcggcacga ctaaatagcg caccgggtca acggccaagg
     1141 tctccaaagc gtcgatttgc tcggtcacct tcattgttcc caactcggcg gcgatggacg
     1201 cgcccacccg ccccgccacc accagggcgg tcagcaccgg ccccaattcg atgaagactg
     1261 ctttgccgac ggtggccccc aggtagcgca acggcacgta ccccttgaac tgataagcgg
     1321 cctgccaggc ggagaccgcg ccgacgaagg tggaggtaat gaggatgagc ggcagtgagt
     1381 tgaccccgat ggcgagcatt tgattggtcg tcaggcggaa acggcggaaa aaatcgggca
     1441 aacccagaac aaagcgcccg gccaggaccg acaacccgcc gacgtccgac aggatgacta
     1501 acgtctggcg gcctaccagg cgcagggcgt gggtcatgcc ggtagtttac aaggagaaaa
     1561 gcgaaagcac aaaagaaaaa tcagggaaga caggtttcag aattgtgaac ttagaattga
     1621 gggataatct tttctctaaa gttttcaaaa aggcgcctgc tcgcccacgt acgccatatt
     1681 ctcaaatcgc gcgtactcgg ggatgaagga gagccgcacc gtaccggtgg gcccgttacg
     1741 ctgcttggcc acgatgattt cggccaagtt ttcggtcggt aaaccctctt cggtggtttc
     1801 gatatcgtac accttcggac gataaatgaa catcaccacg tccgcatcct gctcgatggc
     1861 gcccgattcg cgtaaatcag ccagttgcgg ccggcgcttg ccgccccgca cctccaccgc
     1921 gcgggagagc tgcgaaagcg ccaccaccgg cacgtctaat tccttggcca gtcctttgag
     1981 cgagcgggag atcaaggcga tttcctgctg ccggttctcg gcgttcctgg gcccctgcat
     2041 cagttgcata tagtcaatga ccacgaggcc gatcttgtgc tgggtcttga gccgccgggc
     2101 tttggcgcgc agttccagca cgtccatatt cggcgtgtcg tcgataaaga ttttcgattc
     2161 ggaaagcggc ccgaccgcaa tcgagaggtt ggcccattcc tcgttgcgca gccgcccggt
     2221 gcgcatggcg tggctggaaa cccggccccg ggaacagagc atccgctggg ctaactggtt
     2281 tttggacatc tccagactga agatgaccag tggaatctgg tggtcgcaac ccacgtgctc
     2341 ggcgacgttc agaatgaaag cggtcttccc cattgagggc cggcccgcca ccacgatgaa
     2401 atcggccggc tggagccctg cggtttgcac gtctaagtcg gtaaagccgg ttggcagacc
     2461 ggtgatgttt ccgcctcgct tgtggtattc ctcaatagat tcgaaggtgt ggggcaaaat
     2521 ctcgcccaaa ggcagagcgc tctgccgcac catcgcctcc ttgatgctga agatgcgtga
     2581 ttcggcccgg tctaagagtt catccacctc ttcggtgggg tcaaagcagc gggagacgat
     2641 ttcggtggcg gtctgggcca gcgtgcgcag ggtgtagcgc tctaagacga ttttggcatg
     2701 gtgctccacg tgggctgccg tggccactcc ttcgaggagg gaggcgaggt aggctcggcc
     2761 gccgatggtt tccagttgcc ccgacttgat taaggcttcg gagacggtaa ccagatcaac
     2821 cggctcactt ttctcgtaaa gatcgagcat ggcctggtag atttgccggt gtgcttcacg
     2881 gtagaaggca tcgggagtga tgagactcgc cacccggtca agggcagtct tatccagcag
     2941 gatcgcggct aagacctggc gttcgacctc gactgcctgg ggcggaaggc gttcaccgga
     3001 aagaatatcg tctggcatcg gaaatatcga ttttaatcaa tgatttcgtt tttctaccgt
     3061 tctctccacg ctctggcctt cggcttcgct cagcctgaag agagcgtggc tacaatatta
     3121 accgtgcatc cgaattttac aacacagtcc cgtcatatgc cttccgtagt atcttcatat
     3181 cgtcccaaac atcttttttg tacgagggaa aacgcagaag cgccgccgga tggtaggtga
     3241 caagcacttt tataccttgg taatcgtgga aagcaccgcg cagtttgccc agcgggagtt
     3301 tggtgttcaa aagcgtcacc gccgcaatcc ggcccaggca gacgatgaac tttggcttga
     3361 tcagttcaat ttgcttctgc agatacggaa agcaggcagc gatttcctcg ggcaggggtt
     3421 cgcggttctc cggc
//
LOCUS       DBNGZA010000334         3238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2397978, whole genome shotgun sequence.
ACCESSION   DBNGZA010000334 DBNGZA010000000
VERSION     DBNGZA010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2397978"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC16FU_07365"
     CDS             complement(<1..229)
                     /locus_tag="AC16FU_07365"
                     /inference="COORDINATES: protein motif:HMM:NF017508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR2994224.1"
                     /translation="MPEIVSASTIKVKVTDQGSWRRRLEIEVPPERIAAQFAATYEQF
                     KAKAKIPGFRPGKAPLDLVKERFAGLARQEVL"
     gene            complement(249..333)
                     /locus_tag="AC16FU_07370"
     tRNA            complement(249..333)
                     /locus_tag="AC16FU_07370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(296..298),aa:Leu,seq:tag)
     gene            466..1014
                     /locus_tag="AC16FU_07375"
     CDS             466..1014
                     /locus_tag="AC16FU_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994225.1"
                     /translation="MNLQKNKFIYIISCVFIVATQTQSFPQSAQAIDPWQHWRFLLGE
                     WVATGTGQPGEGVGHFSFTYDLDKKVLVRKSRVDYPPKPGEKTGLSHKDLLIVYQPPG
                     DSLFRAVYFDNEGHSINYAASFPETTLNAATLESEPSQKGPRFRLAYEMNADGTLQVS
                     FSIALPGESFKIYTQGIAHRKP"
     gene            complement(1164..2729)
                     /gene="hutH"
                     /locus_tag="AC16FU_07380"
     CDS             complement(1164..2729)
                     /gene="hutH"
                     /locus_tag="AC16FU_07380"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR2994226.1"
                     /translation="MSSIDGEHLTLDQIVAVARQGERVALSPKAKKKMAAARRFIDQK
                     VARGETIYGVTTGVGSFADTVLERSQAEKFQENLILSTAAGVGAPLAEEEIRALMLLS
                     LNCQAKGHSGSRVEVPLLLTGMLNRKVHPVIPSQGSVGASGDLAPLAHMALVLIGQGE
                     AVYRGRTYPGAKALKRAGLKPIRLREKEGLALINGTHLMTGLGMLALTDAIALVKQAD
                     VVGALTCEAVLARSAPFDARVHRLRPHPGQTATAGNFRKLLKGSRLIDAELPAPAMVQ
                     DVYSIRCIPQVHGAVRDAMAYVRSVLEIEINSTTDNPLIFANDGAVLSAGNFHGQPVA
                     QALDHLSLALTTLGNISERRIAKLLDKNHNNGLPAFLIPNGGLNSGLMVLQYTAAALA
                     SENKVLCYPASADTIPTSANQEDHVSMGPIAARHARQIAVNVANILAIELFCAYQALG
                     LRLSGEAEPTLNAQALSPAAAQVRKLLRKETGLPEVFLKDENFGSYQGNLRALLVNER
                     LAAQVERVCGKLV"
BASE COUNT          741 a          933 c          848 g          716 t
ORIGIN      
        1 ccaacacttc ctgccgggcc agcccggcga agcgttcttt gaccaaatcc aacggcgctt
       61 tgccgggccg gaagccgggg attttggctt tggctttgaa ctgctcgtag gtggccgcaa
      121 actgggcagc gatccgctcc ggcggcactt cgatttccag ccgcctgcgc cagctgccct
      181 ggtcagtcac ttttactttg atcgtgctgg ctgaaacaat ttccggcatt ttggattggc
      241 tctatctatg cgaaaggggg gattcgaacc cccacccctt ttaagggact ggatcctaag
      301 tccagcgcgt ctgccaggtt ccgccacttt cgcgcaatta tactatataa atctgagtgt
      361 aggggaagtc aagaaaatcg atctgttgac agtaaaatcc acttcagcta tagtacgtcg
      421 gccaaaaact gtttcccatc ttcaaccttg aaaaggagta ctaccgtgaa cctgcaaaaa
      481 aataaattca tttacatcat ctcgtgcgtg ttcatagtag caacacagac acagagtttc
      541 ccgcagtccg ctcaagcgat tgatccctgg cagcactggc gatttctcct cggggagtgg
      601 gttgccaccg gcacgggaca gccgggggaa ggcgtcgggc atttttcctt cacgtacgat
      661 ttagacaaaa aggttttggt gcgcaagagc cgcgtggatt atccacccaa acccggcgaa
      721 aagaccggtt tatcgcacaa agatctcctg atcgtttacc agccacccgg agattcgctc
      781 ttccgagctg tttacttcga caacgaaggc cacagcatca actatgcggc gtcgttcccg
      841 gaaacaactc ttaacgcagc cacactggaa agcgaaccgt cgcaaaaagg accgcggttt
      901 cgcctggcgt atgaaatgaa cgcggacggt accttgcagg tttcattttc catcgcgctt
      961 cccggcgagt cctttaaaat ttacacacag ggcatcgcgc accgaaagcc ctaactttga
     1021 taccgggcgg aaaacggctg ctttcttgac cgacgttccc aacagataat ctgcttcagg
     1081 tgacctcttc tctgaccccc caacctacaa agaggagctg atggttttcc ctccccttgg
     1141 aggggaggac gagggagggg tcgtcacacc agctttccgc acaccctttc gacctgcgcc
     1201 gccagcctct cgttgaccaa aagcgcccgc aggttaccct gataactgcc gaagttttca
     1261 tccttcaaga aaacttccgg caatccggtt tctttccgca acaacttccg cacctgcgct
     1321 gccgccggcg agagcgcctg tgcattgaga gttggctctg cctcgccgga gagccgcaga
     1381 cccagcgcct ggtacgcgca gaaaagttcg atcgctaaga tattggccac gttcactgca
     1441 atttgccggg cgtggcgggc cgcaatcggc cccatgctca catggtcttc ctgattggcg
     1501 gaggtgggga ttgtatcggc tgaagcgggg taacagagga ctttgttttc ggaagctaaa
     1561 gccgcggcgg tgtattgcag aaccatcagg ccggagttca acccgccgtt gggaatcaga
     1621 aatgcgggca ggccattgtt gtgatttttg tccaagagtt tagcaatccg cctttcggag
     1681 atgttcccca gcgtggtgag cgccagactc aaatggtcga gcgcctgcgc caccggctgg
     1741 ccgtgaaaat tgcccgccga aagaaccgcg ccgtcgttag caaaaatcaa gggattatca
     1801 gtcgtggagt tgatttcaat ttccagaacg ctccggacat acgccatcgc atcacgcacc
     1861 gccccgtgca cctgcggaat gcagcggatc gaatagacat cctgcaccat ggctggcgcg
     1921 ggaagctcag cgtcgatgag ccggctcccc ttcagcagtt tgcggaaatt tccggcggtg
     1981 gccgtctggc ctggatgggg tcgtagccgg tgcacccggg catcaaatgg agcactacga
     2041 gctaaaaccg cctcgcaggt caaggcaccc acaacgtcgg cctgcttgac caacgcgatt
     2101 gcgtcagtca aagccaacat ccccagcccg gtcatcaggt gggtgccatt gatcaaggcc
     2161 aggccttctt tttcgcgcag tcggattggc ttcagccccg ctctcttaag cgcctttgct
     2221 cccggataag ttctgccgcg atagaccgct tctccctggc caatcaacac taacgccata
     2281 tgcgccagag gcgccaggtc gccggaggcg cccacggagc cttgagaggg aatgaccgga
     2341 tgcaccttcc ggttcagcat tccggtcaac agaaggggaa cttcgacccg actgccggag
     2401 tgtcctttcg cctggcagtt gaggctgagg agcatcagag cgcgaatctc ttcctcggcc
     2461 agaggcgcac ccactcccgc cgcggtggag aggattaagt tttcctggaa cttttccgcc
     2521 tggctccgct ccaggaccgt atcggcaaaa gaaccgacgc ccgtggtgac gccgtaaatc
     2581 gtctcccctc tggccacctt ctggtcgata aaccgccgcg cagccgccat tttctttttc
     2641 gccttcgggg atagcgccac tctttccccc tgccgggcga cagcgacaat ttgatctaac
     2701 gtgagatgtt cgccgtctat cgaagacatg gtgagggaat ttatactatt ttagaaaaag
     2761 aaaggaggga aaaacaaatc gatcgaggcg aagggcactt ctaaacttca gtaaacagta
     2821 ccaacgaata atctcgtatt cgcgctttgt gcaataccga tgttcccgtg cgcaagttat
     2881 tgcatcaaat acgttttgga attcctgaaa actttaattc tcagccgctt agtcggttcg
     2941 gtcaataaga taatcgtaag tggcacttcc acttaaaggc cgtaagaagc atccctctaa
     3001 ccgaaaagat acaggtattg aaattgctgc gattcgttta tatcttagtc ttttcgcaat
     3061 tataaaagag gagtgcccgt ctcaatgctg caaaacagtt tggcaccact actgcagagg
     3121 aggaaacgat gaaaggcaaa accgcactgc tcgcgctcgt gatcgccctg gtgatcggcg
     3181 gttgcagcag gcagaactcc atcggcccat ctccggccga aacgcagtta gcgggaac
//
LOCUS       DBNGZA010000335         3927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_65727, whole genome shotgun sequence.
ACCESSION   DBNGZA010000335 DBNGZA010000000
VERSION     DBNGZA010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_65727"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16FU_07385"
     CDS             <1..447
                     /locus_tag="AC16FU_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR2994227.1"
                     /translation="LSAGLAGTTVLLDVGEEVTNLAVGKDGELDYIEALTTPPGLFDT
                     PPRQLNEGFVLLKSELTRALAEEVATLLRSQGPDLQNGKIALSGPGASDAEIQKAIAT
                     VLSLPVEPANLLAACDWSSELKPKREDFLSQSHRYSVALGLALAPH"
     gene            619..1188
                     /gene="rsmD"
                     /locus_tag="AC16FU_07390"
     CDS             619..1188
                     /gene="rsmD"
                     /locus_tag="AC16FU_07390"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR2994228.1"
                     /translation="MRIIAGFAKKRKLFTEPRQRRSLRYSSDKTKGAIFNILGATVVC
                     ARVLDLYAGSGNLGLEALSRGAAGVTFVEKRFDAVNLIKRNAALLGVAEKITVLRGDV
                     QTVVERLAHQGQAFDLVLADPPYREGLCRKLVILLSGNSVLKPEGFLVCEHDKREVLP
                     EQFETLALVKHRVFGDTALSIYQYRPGAA"
     gene            1265..1744
                     /gene="coaD"
                     /locus_tag="AC16FU_07395"
     CDS             1265..1744
                     /gene="coaD"
                     /locus_tag="AC16FU_07395"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR2994229.1"
                     /translation="MKIAIYPGTFDPITNGHLDLIKRALELFDELVVAIAENPEKHPL
                     FTLEERLEMVKATVASLSGVKVMTFAGLTAELTTKAGAVAILRGLRAVSDFEFEFQIA
                     LMNRRLAPKAETVFLMPSEQFTYLSSNVIKDVARHSGDITAFVPPIVAQKLKAKFKS"
     gene            1828..>3927
                     /locus_tag="AC16FU_07400"
     CDS             1828..>3927
                     /locus_tag="AC16FU_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR2994230.1"
                     /translation="MLGKIVSHYKIVEKLGEGGMGVVYKALDLKLHRPVAVKFLPPHV
                     ADSPDEKARFLHEAQSASALNHPNITTIYGIEETPEGIFIAMEYVEGKTLKQVVEKET
                     LPLKKILEIASQVCEGLTAAHKKEVVHRDIKSENIMVTREGLVKLMDFGLALLKGTSR
                     LTRAGSTLGTAAYMSPEQASGEEIDQRSDIFSFGVVLYELLTGKLPFPGEHQAAIIFS
                     ILNEQPQPVARYNSKVSPELERIVAKTLAKDKEERYQHIDDLLADLRREKKQLEYVKA
                     SQIPPEAAAARPKKKLLPILVPASLVFAAAFLFFILNPFKIEIGSEKKATAAENSLAV
                     MYFDNVADPEDRDRIVQMITALLITDLSESQYLQVVSRQRLYDILKLVGKENLKSIDK
                     SVASEVAQRAGVKWVLTGSVLQTKPNIVLVSDIADAASGKVRASERITGEAGQDIFAV
                     VDRLSAEIKKDLALPTQAAQEPDKPVADATTHSPEAYRYYLEGVDYWEKLYDPEAVKS
                     FRKAIELDSTFTMAYFYLARVLTRLQNPDAKKAIAQAVKYSNKVSEMERHYIRALEAR
                     LAGNFPLAIKEWEKIAADYPQEKDAYISLGATYANALQQPEKAVGPLTKAIEIDPLVK
                     AAYNLLAYAYNDLGDFEKSIWAINKYISLAPEEANPYDTRGDLYAYNGKLDEAIESYR
                     KALEKKPDFYSSLTKLGY"
BASE COUNT          915 a         1098 c         1105 g          809 t
ORIGIN      
        1 ctctctgccg gtttggcggg aacgacggtt ctgctcgacg tcggcgaaga ggtgacgaac
       61 ctcgccgtgg ggaaagacgg cgaactggat tatatcgaag cgctgacgac tccgcccgga
      121 cttttcgaca cgcctccccg gcagttgaac gagggcttcg ttctcctcaa aagcgagttg
      181 acgcgcgccc tggctgagga agtggccacc ctcctccgca gtcaaggccc cgacctccaa
      241 aacgggaaaa tcgctctctc cggccccggc gcgagcgatg cggaaataca aaaggcaata
      301 gccacggtcc tctcccttcc ggtggaaccg gccaacctct tagcggcctg tgattggtca
      361 tccgagctca agcccaagag agaagacttt ctttcccaaa gccatcgcta tagcgtggcc
      421 ctgggcttgg cgctggctcc ccattgattt cctccatttc ccccaaggtt cgttgaccca
      481 tttatccttg agccggtcct agactgccga aggtaaacga ccgtgctcgg agaaaacgag
      541 cctttctctt tgagtggaaa tttccctaag ggggcgctat attttcagac ggaaggaaat
      601 ggctgcgggt ttttgacgat gcgcatcatt gccggtttcg ccaagaaaag gaaactcttc
      661 accgaacccc gccagaggcg gagcctgcgc tattccagcg ataagaccaa aggtgccatt
      721 ttcaacatcc tgggggcgac ggtcgtctgt gcccgggtgc tcgacctcta tgccggatcg
      781 ggaaacctcg gtctggaagc gctctcgcgg ggcgccgcgg gggtaacctt tgtggagaag
      841 cgttttgacg ccgtcaatct gatcaaacgc aatgccgccc ttctgggagt cgcggagaaa
      901 atcaccgtcc tgcggggaga tgttcagacc gtcgtggagc gtctggcgca ccagggtcag
      961 gcgttcgatc tcgtcctggc cgacccgccc taccgggaag gtctgtgccg gaagttagtc
     1021 atcctgctct ctggaaactc cgtgctgaaa ccggaaggct ttctcgtctg tgagcacgat
     1081 aaaagagagg tcttgccgga acagttcgaa acgctggcgc tggtcaagca ccgcgttttc
     1141 ggagacaccg cactttcaat ttatcagtac cgacccggag cggcatgaat tccgctctgc
     1201 cagccggaaa aaaggaacag gttccggatc cggcgatccg gggcggcctt cccgggaggt
     1261 atccgtgaaa atagccattt accccggcac gttcgacccc atcaccaacg gccatctcga
     1321 cttgatcaag cgggcgctgg agttgttcga cgagttggtt gtcgccattg cggaaaatcc
     1381 ggaaaagcat ccgctcttca ctctggaaga gcggctggaa atggtcaaag cgacggtggc
     1441 gtctctttcc ggcgtcaagg tgatgacctt cgccggtctg accgccgagc ttaccaccaa
     1501 ggccggggcg gtggcaattc tgcgcggcct gcgcgcggtt tccgattttg aatttgaatt
     1561 ccagatcgcc ctgatgaacc ggcgtttagc ccccaaggcc gaaacggtgt tcctgatgcc
     1621 gtctgagcag ttcacctacc tctcttccaa cgtgatcaaa gacgtcgccc gccactcagg
     1681 cgatatcacc gcttttgtcc cgcccatcgt agcccagaaa ttgaaagcca aattcaaatc
     1741 ctgactcacc ggaattttct gctgcgggga actcaggtgg cccagtcatg ttgctttggg
     1801 cactaaacta tcctactttt atcccttatg cttgggaaaa ttgtctccca ctacaaaatc
     1861 gtagagaaac tcggtgaagg ggggatgggg gtggtctaca aagccctgga cctcaaactc
     1921 caccggccgg tggcggtgaa gtttctgccg ccgcacgttg cggatagtcc cgacgagaag
     1981 gcgcgcttcc tgcacgaagc gcagtcggcc tcggctctca atcaccccaa catcaccacc
     2041 atctacggca tcgaggagac gcccgagggg attttcatcg ccatggagta cgtggagggg
     2101 aaaacactca aacaggtggt ggaaaaggaa accttgccgc tcaagaagat tctggagatt
     2161 gccagccagg tctgcgaggg gttaacggcg gctcacaaaa aggaagtggt gcaccgggac
     2221 atcaagtcgg agaacatcat ggtcacccgg gaggggctgg tcaagctcat ggatttcggc
     2281 ttagccctgc tgaaaggcac cagccggttg acccgggcgg ggtcaacctt agggacagcg
     2341 gcctacatgt ccccggagca ggcctcgggg gaagaaattg atcagcgctc cgatattttc
     2401 tccttcggag tggtgctgta cgagcttctg accgggaaac tcccctttcc gggcgagcac
     2461 caggccgcca tcatcttctc catcctcaac gaacaacctc agccggttgc cagatacaac
     2521 agcaaagttt cccccgaatt ggagcgcatc gtcgccaaga ctttagcgaa ggacaaagag
     2581 gagcgctacc agcacatcga cgacctcctg gccgatttgc gaagggagaa aaagcagctg
     2641 gagtacgtca aggccagcca gattccacct gaagccgcgg cggccagacc gaaaaagaaa
     2701 ctcctcccca ttctggtacc cgcctcgctc gtttttgccg ccgccttcct attcttcatt
     2761 ctcaatccgt ttaaaattga gatcggctcg gaaaagaagg cgaccgcggc cgagaactcc
     2821 ctggcggtga tgtacttcga caacgtggcg gatccggagg atagggaccg gattgttcaa
     2881 atgatcaccg cattgctcat caccgacctt tccgaatccc agtacctgca ggtggtcagc
     2941 cggcagcggc tgtacgatat tctgaaactc gtcggcaagg aaaacctgaa atccatagac
     3001 aaaagcgtgg cctcggaagt ggctcaaaga gccggggtga aatgggtttt gaccggcagc
     3061 gttttgcaga ccaaaccgaa tatcgtttta gtttcggata tagccgatgc tgcttcaggc
     3121 aaagtgcggg ccagcgaacg cattaccgga gaagcggggc aggatatttt tgccgtggtg
     3181 gaccggcttt cagcggagat aaagaaagat ttagccttgc cgactcaagc ggcccaggaa
     3241 ccggacaagc cggtggcgga tgcaaccact cattccccgg aggcctatcg ctactacttg
     3301 gaaggcgttg attactggga aaaattgtat gatccggagg cggtaaaaag tttcaggaaa
     3361 gccatcgagc tggattccac ctttaccatg gcctatttct atttggcccg cgttctcacc
     3421 cgactccaga atccggatgc caaaaaagcg atagcccagg ccgtgaaata ctcgaacaag
     3481 gtcagcgaga tggagaggca ctacataaga gctttggagg cgcgtttggc cggtaatttt
     3541 ccgctggcca taaaagagtg ggagaagata gcggccgatt accctcaaga aaaagacgcg
     3601 tatatcagtt tgggggccac atatgcgaac gcgcttcagc agccggaaaa agcggttggg
     3661 cctttgacca aagcgattga aatagacccc cttgtaaagg ccgcgtataa cctgctggcc
     3721 tatgcttaca atgacttggg agattttgag aaatccatct gggcgataaa taaatatatt
     3781 tccctggcgc cggaggaagc caatccttac gacacccggg gcgacctata cgcctacaat
     3841 gggaaattgg acgaggcgat tgagtcctac cggaaggccc tggaaaaaaa gccggatttc
     3901 tattcgtctc tgactaaatt ggggtat
//
LOCUS       DBNGZA010000336         3636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2398362, whole genome shotgun sequence.
ACCESSION   DBNGZA010000336 DBNGZA010000000
VERSION     DBNGZA010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2398362"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1241
                     /locus_tag="AC16FU_07405"
     CDS             108..1241
                     /locus_tag="AC16FU_07405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2994231.1"
                     /translation="MRIAFDATSLARPQTGIETYTRHLLAALLREKSAHAWVVLLRGG
                     MPEELRELAQRGRWLNCPANSQLFCEQVWIPRVLAKERVDLAHFPAFPPSPLVSVPFI
                     YTAFDATLWRNWPLLSWKGKFYFRPLADLALRRARKVLTISESSRRDLQSFFPGLNGT
                     VANAGIGISEDFFTSRRLTRSDAEAVRRRYRLPQRFILSVGSLEPRKNLKTLLWAFAR
                     YGELHPADKLSLVLAGRPAWGAAEVAGLQKHPALRNRLVFTGYVPQVDLPKIYRLATM
                     LVFPSRYEGFGLPVAEAFASGTPVVASQIPAVVEQADGAALLVDPFDVEGFAQAMARL
                     SADGKLRRRFAAQGRAAAARYRWQKVAEQVLAIYAEVGQCRKK"
     gene            1226..1981
                     /locus_tag="AC16FU_07410"
     CDS             1226..1981
                     /locus_tag="AC16FU_07410"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR2994232.1"
                     /translation="MPEKVTLLDLTIDNVDLGEAIGRIEQLVAGRRGAFVVTPNVDYM
                     VNLRRDQKFRQIVNEAQLVLADGMPLVWASRFLGTPLKERVTGADLFPKLLERAAKHH
                     WKVFLLGGKPGVAQRIARRVRERFSGLPLVGCYSPPFGFENDVYALGEIAALVGAARP
                     DLLCVGLSSPKQEQLIVYLRQKEKIPLIVGVGAAFDFAAGTLRRAPVWMQRVGLEWAF
                     RLASEPRRLWRRYLRDTMFFWYVLQQKLSSRTA"
     gene            complement(2098..>3636)
                     /locus_tag="AC16FU_07415"
     CDS             complement(2098..>3636)
                     /locus_tag="AC16FU_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994233.1"
                     /translation="GQSGNSVTSGVRGATIGGGGTIAFTNNQVTDDFGTIGGGDANQA
                     GDNAGTTSDRTHATVGGGFSNTASGDDATVGGGNTNTASGGGATVGGGILNTASGSRA
                     TIGGGFSNIASGRFATVGGGLSNTASGDFSFAAGRRAKADHLCTFVWADSSADADFAS
                     TADNQFLIRAVNVGIGTNNPANKLDVEGGAVIGATYSGTNLAPTNGLLVEGNVGIGTT
                     GVSAQLDVEQLGATVANFNRSSTDGTLLQFQRDAVTVGSISVAAGVVSYNAFTGSHYA
                     WTDEVLGRGELVTLTGINGNSHNDPESEVIYGIKRSTVANDPACLGSYLALQELSQPA
                     STENPHLVMAVGNGDMWVTDESGDIHPGDYLISSSTPGHAMKDDEAKYPLGYVIARAA
                     DPVNWGNESEMVGGHKHKRISVLFESFVRNSTTDLVKLVETQQETLTKQQKEIDELKA
                     LVKTLVAKEQPDGKSIGQASSPARGGTSSLTETVRQLNIYFGMSPYGSIGIAPTLPLL
                     RGKL"
BASE COUNT          678 a         1066 c         1068 g          824 t
ORIGIN      
        1 gaaaattttc tttgctggct tggcgaatgc ctcctttctc atttccccca tctggccctg
       61 gcagtcgggc gaaaattttg tcggtatctt ccggaagaac ggataggatg cgcattgcct
      121 tcgacgcgac atctttagcc cgcccgcaga ccggtatcga gacctacacg aggcatttgc
      181 ttgcggcgct cctccgggaa aagtcagcgc atgcctgggt ggtgttgctg cgcggaggga
      241 tgccggagga attgcgtgaa ctggcacaga gagggcgctg gctcaactgt ccggccaact
      301 cacaactctt ctgcgaacag gtctggattc cccgtgtgct ggcgaaggag agagtcgacc
      361 tggcgcactt ccccgccttt ccgccgtcgc ctttggtctc ggttcccttc atctacaccg
      421 cttttgatgc caccctgtgg cgcaactggc cgcttttgtc gtggaaaggg aagttctact
      481 tccgtccgtt ggccgacctg gcgctccggc gcgcccgaaa agttctgacg atttccgaga
      541 gttcccggcg cgacttgcag agcttctttc ccggtctgaa tggcacggtg gccaacgccg
      601 ggatcggcat cagtgaagat tttttcacca gccgaagact gacccgaagt gatgcggaag
      661 cagtccgccg ccggtaccgt ctgccgcagc gcttcattct ttcggtgggg tcgctcgaac
      721 cgcgcaagaa tctcaaaaca ctcctttggg cttttgcgcg gtacggtgaa cttcaccctg
      781 cggataaact cagtctggtc ctggcggggc gacccgcctg gggcgcggcg gaggttgccg
      841 gactgcaaaa acacccggcg ctgcgaaatc ggctggtttt taccggctac gtcccgcagg
      901 tcgatctccc caaaatctac cgactggcga cgatgctggt gttcccgtcg cgctacgagg
      961 gattcggtct gccggtggcc gaagcttttg ccagcggcac gccggtcgtc gcctcgcaaa
     1021 ttccggcggt ggtggaacaa gcggatgggg cagctcttct ggttgatcct ttcgacgtgg
     1081 aaggattcgc acaagcgatg gcgcggctct cagccgacgg gaaattgcgg cgccggtttg
     1141 cggcccaggg acgggcggcc gcggcgcgct accgctggca gaaggtggcc gaacaggtgc
     1201 tggcaatcta cgctgaggtc gggcaatgcc ggaaaaagta acgcttctgg atttgaccat
     1261 cgacaacgtc gatctcggag aagccattgg ccgtatcgag caactggtgg cagggcggcg
     1321 gggcgccttt gtggtcactc ccaacgtaga ctatatggtc aatctgcgcc gcgatcaaaa
     1381 gttccgccaa atcgtgaacg aagcgcagct cgtcctggcg gacgggatgc ctttagtctg
     1441 ggccagccgg tttttgggca caccgctcaa agagcgggtt accggcgccg atcttttccc
     1501 caaactcttg gaacgggcgg ccaaacatca ctggaaagtc ttccttttag gcggcaagcc
     1561 gggcgtggcg cagcggatag cgaggagggt ccgggagcga ttctccggtc tgcctttggt
     1621 cggctgctat tcaccccctt ttggtttcga aaacgatgtc tacgcgttgg gagaaatcgc
     1681 cgcgctggtg ggggcggcac gccccgacct cctctgcgtc ggattatcct ctcccaagca
     1741 ggagcaactc atcgtctacc tcaggcaaaa ggagaagatt cccctcatcg ttggtgtagg
     1801 ggcagccttc gactttgccg cgggcacgtt gcggcgggcg ccggtttgga tgcaaagggt
     1861 tggcctggaa tgggcctttc gcctggcctc cgaaccacgc cgcttgtggc gccgttacct
     1921 gcgcgatacg atgtttttct ggtacgtcct gcagcagaag ttgtccagcc gaacagcgta
     1981 gaatcaagtc gaggaactgc gaagctccgt gcaggtttcg aaaccaaccc cgccttgggc
     2041 cccgccgttg gcgggatctg cgacgggatc atcgacctgc actactggtt tctgctccta
     2101 caatttccct cgcaaaagag gtagagtcgg tgcgatccct atggatccgt atggactcat
     2161 gccgaagtag atgtttagtt gtcttacggt ttcagtcagg ctggaagtcc cgccacgtgc
     2221 gggactggaa gcctgaccta tggactttcc atcgggttgt tcctttgcca ccaacgtttt
     2281 taccagcgct tttaattcgt cgatttcctt ttgctgtttg gtcagagtct cttgctgggt
     2341 ctcaacgagc ttcacgaggt cggtggtgct gttgcgcaca aaactttcga acagaaccga
     2401 aatccgcttg tgtttgtggc caccaaccat ctcgctctcg ttaccccagt tgaccgggtc
     2461 ggcagcccgg gcaatcacat agcccagcgg gtattttgcc tcgtcgtctt tcatagcgtg
     2521 gccgggagta gatgaggaaa tcaggtaatc gccggggtga atatctccgc tttcgtctgt
     2581 cacccacata tccccattgc ctacggccat aaccagatgg ggattttccg tgctggccgg
     2641 ttgagacagc tcttgaagcg caaggtagga acccaggcag gccgggtcgt tggcgaccgt
     2701 tgaccgcttg attccgtaga taacctccga ttcggggtca ttgtgagagt tgccgtttat
     2761 acccgtaagg gtaaccagct ccccccgccc cagaacttca tccgtccagg cgtagtgcga
     2821 gccggtgaag gcgttgtagc tgactacacc ggcggcgacg gaaatggaac ctacggttac
     2881 agcatctctt tggaattgga ggagggtgcc gtcagtggag ctgcgattga aattggcaac
     2941 ggttgctcca agttgctcca cgtccagttg ggctgacact ccggttgtcc cgataccaac
     3001 attaccctct accaaaagac cattggtcgg ggcgagatta gtgccggagt aagtcgcacc
     3061 gataaccgct ccaccttcaa cgtctaattt gttggccggg ttgttagttc ctattccaac
     3121 atttacggct cggattaaaa actggttgtc tgccgtggag gcgaaatcgg catcggcgct
     3181 gctatccgcc caaacaaaag tgcagaggtg atcggccttt gcccgccgac cagcggcaaa
     3241 agaaaaatct ccactggcgg tgttgctaag ccctccacca acggtggcga atctcccgct
     3301 ggcgatgttg ctaaaccccc caccaatggt ggcacggctc ccgctggcgg tgttgagaat
     3361 tcccccacca acggtggcgc cacccccgct ggcggtgttg gtattccccc caccaacggt
     3421 ggcgtcgtcc ccactggcgg tgttgctaaa ccccccacca accgtggcat gtgttctatc
     3481 gctggttgtg ccagcgttgt ctccagcttg attggcatcg ccccctccta tcgttccaaa
     3541 gtcatcggta acctggttgt tggtgaatgc aatcgtccct ccgccgccaa tagtagcgcc
     3601 gcgaacaccg gaagtaacgc tgttgccact ttggcc
//
LOCUS       DBNGZA010000337        14434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2184921, whole genome shotgun sequence.
ACCESSION   DBNGZA010000337 DBNGZA010000000
VERSION     DBNGZA010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 14434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14434
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2184921"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..957)
                     /locus_tag="AC16FU_07420"
     CDS             complement(91..957)
                     /locus_tag="AC16FU_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR2994234.1"
                     /translation="MRARSQPYLLASPWILTLLLFWLFPLVWSLYLSFTNYGILNPQS
                     DWIGLANYQKLFRDPAFWIALKNTFIFVIGTVPVTTVLALAFALALNQKLPWRGLFRA
                     GFFIPSITSLVVVALVFTNLYSQNGYLTLLAKAAGIETRGLLLSEKTALPAIMLMDVW
                     AAVGYYLLLFLAALQAIPQELYEQADISGASRWQKFRHVTLPHLRPMFLFVVVINAIK
                     SFQVFTEIFVMTKGGPLSATTTSVYQVYEEGLQRFQMGYASALAYLLFLIIAGFSFLA
                     ARLFRLGKGIAD"
     gene            complement(964..2208)
                     /locus_tag="AC16FU_07425"
     CDS             complement(964..2208)
                     /locus_tag="AC16FU_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR2994235.1"
                     /translation="MQMKFRFWLVFVVAGYATVLMSACGRQQAEPVTLTWWQFRTDRE
                     TRPLIDSLVQKFEKRYPKIKVAVTDLTWEAGHDKIAIAFGADAAPDVIEFGSDWVPEF
                     ITAGKLYDLTDSVADLKEQLLRWEPVTRNERLYGVPWTLGTRVLFYNRTLLQKAGFTG
                     PPQSWSELLLQARTINALGSQIYGFGANANERHRLYKKFLPFLWANGGQILADDQSRS
                     LLDEPRSVAALEFYTKLCDAGLMATQTELDDTFCAGRLGFVISGDWLLKRLTKTPPAF
                     EFGTALLPRPRSDSGPPASFAGGEYLAVNAKTKNLSAALAFLRFLIAREINLEFCQKA
                     GLSNPANRDAAADPYFQSDPNRKTFIEQMQFSVFPPFHPKWVYIEEAIEKAVEEAMYN
                     KKSPRQALVDASATIDALLKSP"
     gene            2335..3216
                     /locus_tag="AC16FU_07430"
     CDS             2335..3216
                     /locus_tag="AC16FU_07430"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR2994236.1"
                     /translation="MGKRSIHRRWSSVNFFFLFFLAFTLAGEPAGSAVSESMGFQTDR
                     LPILVGFLVCAAVLLLTLTLGRKRKGYVRPIPGLAAVDEAVGRATEMGRPLLFTSGTG
                     EVDRMATLAALSILGTVAKTAAEHDTRLIYPNNDPVVAAVAQEVVKESCLRAGRPEAF
                     QSEDIFFVTKSQFGYAAAVDGIMVRQKPAACLFFGTFEGESLIFAETGNGIGAIQIAG
                     SDSTIQLAFFLVACDYTLIGEELFAASGYLSGDPVIVASLRGQDYLKLAVIGFLLLGA
                     LAATLHIADLAKLLGGG"
     gene            3213..3836
                     /locus_tag="AC16FU_07435"
     CDS             3213..3836
                     /locus_tag="AC16FU_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994237.1"
                     /translation="MSRRLPLWIAFGFGVLLLVQYFFRHPVATGFYRNTLDWMQVVAV
                     FSLIVGATALAGMHLKAVAARSRGWGYNLVTLAGLGGMIFCGALWGIDEHSPFTWAFL
                     NLQVPLQSTMFSLLAFFVASAAYRGFRARSWPAAVLLLAAAIVIMGRVPVGAQILAGL
                     PQAADWVLNVPSMAARRGILIGIGLGAIATSLRVILGIERTYLGKEG"
     gene            3838..4347
                     /locus_tag="AC16FU_07440"
     CDS             3838..4347
                     /locus_tag="AC16FU_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR2994238.1"
                     /translation="MTFLVLLLVIVNLALLYLYLKKRGTPRWEREKSEIQQVMKEITQ
                     ALRAKELKAISRYLADDFTAFLDSPYRCDSRKAYLAELSALFEHGGIEERSFRQEKVQ
                     VINENCAVVTYHFTEKGTRKDKLYERQGKATEVFRRQPEGWVAVHFHHTLVDETKKPF
                     HFPMAEPGV"
     gene            4344..5156
                     /locus_tag="AC16FU_07445"
     CDS             4344..5156
                     /locus_tag="AC16FU_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994239.1"
                     /translation="MTFFRFDRRYLFGFMALAVAVPLLFPLHLPNEVTPDVRHFYTLI
                     DSLPAGSVVLVSFDHEASTIVEVRPPATAVIRHLFQKDIKIIGVSLLAEGTGIGRELL
                     LRLGREYGKQEGTDFAFLGFRPQYAATISGLGEDIRRVFPTDYRDRPLDSLPLFSSVR
                     NLADLALVISVADGDLPATWVDYAVARYHQKVAAAVTAVMATTYYPYLNSGQLVGLVS
                     GLKGASEYELLLHKPGGGVRGMDAQSVAHLALLLLIGLGNLAFLASRKRAAR"
     gene            5153..5749
                     /locus_tag="AC16FU_07450"
     CDS             5153..5749
                     /locus_tag="AC16FU_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994240.1"
                     /translation="MNVETLIAAILTLAIFSFLYRDNPLYKFAEHLLVGISVGYSVMV
                     AVFSTIGPKLFSPLGHRPTPAAFVSLLLALFLLARLVPRWAWVSRLALSLMIGAGAGL
                     AIPAMLQARILSQLQASLLPLTSLNNILIVVGIVTTLLYFYFSREQKGVWKGTAVVGT
                     YFLMVFFGATFGNTVMSRISLLIGRLEFLLGDFLGLIR"
     gene            6028..6999
                     /locus_tag="AC16FU_07455"
     CDS             6028..6999
                     /locus_tag="AC16FU_07455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR2994241.1"
                     /translation="MILRTFILKVAGSVLAGLLLFPEPGRAQAAQSDSAPASRTAPTA
                     NPAPPKKKPAKRRRPVRPKKPAPELSLTARAAAVVDDRTGLVLYEKNLTCVRPIASLT
                     KLVTALVFLEADPNLDSVITMEKADRARRGRSSLAVGESFALGDILYACLMASDNRAT
                     KALVRSSGLGPDDFIHRMNEVAREVGMDSARFFEVTGLDNRNVATALDCIRLVHAALE
                     NPLIARITATERHTFYSANWHRSHTIFTTNRLVRQKWDVVGGKTGFIGQAGYCLVTRV
                     RNSDRQEVTACILGSRSNNRRFADARKLVQWAFKKLAAPEAPAADTP"
     gene            complement(7123..7195)
                     /locus_tag="AC16FU_07460"
     tRNA            complement(7123..7195)
                     /locus_tag="AC16FU_07460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7160..7162),aa:Phe,seq:gaa)
     gene            7475..8134
                     /locus_tag="AC16FU_07465"
     CDS             7475..8134
                     /locus_tag="AC16FU_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HIR2994242.1"
                     /translation="MGITLKIGTFVAMTAVIILAFVLPAPQAKIGEASRIFFFHVPAA
                     IVASLAFLMAMAYSIGYLKSRSVQYDLKAATTAELGLLFSIVATVSGAIFAKVTWGTY
                     WNWDPRETSMFVLILIYGAYLTLRSAIDAPERRAALSAVSAILAVAPAMFLIFVVPRV
                     YRSLHPTDTIVNVKGESGMSPEVRAVFYLSIATFVLLYFWVFRLSTRIKMRTLRSEAA
                     A"
     gene            8131..8238
                     /locus_tag="AC16FU_07470"
     CDS             8131..8238
                     /locus_tag="AC16FU_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HIR2994243.1"
                     /translation="MTSNYTALVVTLVTWLGLFFYLWRLDRRVKRWEDK"
     gene            8235..8621
                     /locus_tag="AC16FU_07475"
     CDS             8235..8621
                     /locus_tag="AC16FU_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE
                     domain-containing protein"
                     /protein_id="HIR2994244.1"
                     /translation="MKPKYVFGIVLLAAFIVFGAISFKKSLTPYVTFAEAMKARGSVQ
                     VIGQLVKEKTAYDTTAQVLRFTLADDHGHTLPVTYHGIKPGNFEQAISIVAIGRCEGE
                     AFDADQLLVKCPSKYQGEEVKKYDAR"
     gene            8608..10899
                     /locus_tag="AC16FU_07480"
     CDS             8608..10899
                     /locus_tag="AC16FU_07480"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR2994245.1"
                     /translation="MMLGNFLLALATIACAFSLVGYLLSLRGGRGVLALARGSYYVLA
                     ASTTLALGTLVYLFMTHRFDVAYVASYSSKDLPWYFEFSSLWAGQQGTFLLWLFWSSL
                     VGVILLRTTKETESWVMTFYLGIQVFFITILLKRSPFLTHAAPPADGQGLNPLLQDYW
                     MTIHPPVMFLGFALTAVPAAYAMAALASRNYDRWVERARPWATTTLAILGTAIIMGGY
                     WAYKTLGWGGYWGWDPVENASLMPWLATIALVHGFMLQKQRSALRRTNLTLALSAFLL
                     VVYGTFLTRSGVLADFSVHSFVDLGINGFLVIFLGVFAVGAAGLLLWRSRQIKSERLV
                     KNLLAKEFSVFLSLVLLVVTTIIVLLGTSAPLLTRIWGQPGNVNTVYYVRTSLPIGIL
                     LAVVLGVMPYLLWRGSGLQVLALRALWPAVAGLAIVGLSILLGFRSPLYILFGFFAVF
                     ALLSNFLVFYRTFQLKPAYVGGHLTHVGVAFMLLGLITSNVYSTSEKITVTRGESKKA
                     FGYQFTYQGLAGPVEEKDNAVHVLVTRGQKSFTATPKFFWSEYNRDFMRKPYIKRAVF
                     SDLYISPMAHSVMDRAGQNTLALKQGETKEAYGYKITFENFEVLEHDQEKGLIKVGAV
                     LSVAPYGRKAVVGSPGPQRREPVFVLVGEEQSLIPDTLPGGELIYLAAPKPETKEVVL
                     GFSGLNQAQAAAADYVVLDISRKPLIHLLWIGTTCVALGALTASYRWGRRRMGTKELS
                     VRQVGQAPPLSAEVKEPTLATRV"
     gene            11332..12654
                     /locus_tag="AC16FU_07485"
     CDS             11332..12654
                     /locus_tag="AC16FU_07485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR2994246.1"
                     /translation="MPDFESPLEHLESVDRLRSTIEAKISELSEANKLHKRKIFDLYT
                     VFEITRHLNTVLDRDILFDGILLACLGQMGVQGAAIAVPDPENFRIVKTKGTFAPDGK
                     PLQFAPTGAFVRLFTDEPAPLTVLQLQNRLPGFPEVSALERAGVALVAPMLVKAHLEG
                     LLLLTNKLSGHHFTEGDLEFLAILANQLAVAVENARLFASEKQAYQKLREMQGQLVQA
                     EKFAAIGELAASIAHEVNNPLGIIKNYLVLLARAVPGGEAAQEYVAIVQEEVDRIARI
                     VRQFLDLYRPQAEQMVAVDVGTIIDETLIFVGGQLKNQNIRVNRLANGPVPKIVGSGE
                     QLKQVFLNLLMNAKDFMPTGGQIDINVGAGNGRVKVEIADTGCGIPEETLAKIFEPFF
                     TTKKDNNGTGLGLSVCYGIVTRHHGTIRAKNRVPHGACFTLEFPVAQE"
     gene            12711..14366
                     /locus_tag="AC16FU_07490"
     CDS             12711..14366
                     /locus_tag="AC16FU_07490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR2994247.1"
                     /translation="MNVGDPIPELRFPGDPACVPACILIVDDEERMCASLAALLSRAG
                     YNVEATAEAEGALQWLKTRTYDLVIADIKMPGVDGLELLRVARQADPDALVILMTGYG
                     SLDSAVAAIGSGAYDYLLKPIEFPALELVVKRAFEKRRADLSRRALLAELQAKNELLS
                     RKFAQLDALYQAARSLAVARDLDELLVKIVTLATEVIGARVGSIMLVEPETQGLVVKA
                     ALGLEPEIVRTTRLPFGSSIAGYVAQRSEPVVVADVETDGRFARVAKAKYETMSLLCA
                     PLVVKQEVLGVINLADKRGGGVFGPDDLQLLVTFASQVAVAIDDARQIEQALQNARRL
                     VTMGSLLSEISHDLKAPLTNIRGSLQILREKLTRSPEAAEILKATEREVFRLTELVKE
                     LVDFSNPNKYHLQRADLSGVIRRALKLVESDLAKKKIDLQLRVTADLPPIFLNENELI
                     EMLLNLVTNAFESMPEGGLLEVGAQGEQGGVRLWVKDSGGGILPERLPRIFERYYTTK
                     PTGTGLGLPIVERIVKAHNGTIAVESTPGTGTTFAVTFPAAER"
BASE COUNT         2795 a         4217 c         4310 g         3112 t
ORIGIN      
        1 accaccatcc accccaaagg gaagagcagg gtgagcaaaa ggaggctcaa gagcgcagtc
       61 cagaagattt tcgcggttcg gcggggcatc tcagtcggca atccctttcc ccagccgaaa
      121 caagcgcgcg gccagaaaac tgaaaccggc aatgatgagg aacaggaggt aagccaaggc
      181 cgaagcgtac cccatctgga agcgctgcag gccctcttcg tagacttggt agacggatgt
      241 cgtcgtggcg gagagcggcc cgcctttggt catgacaaaa atttccgtga atacctggaa
      301 cgatttgatg gcgttgatca ccaccacgaa gaggaacatc ggccgcaggt ggggcagggt
      361 gacgtgccga aatttctgcc agcggctggc gccggaaata tcggcctgct cgtagagttc
      421 ctgcggaatg gcctgcagcg cggccaaaaa caaaaggagg tagtagccca ccgcggccca
      481 gacgtccatc agcataatcg ccggcagggc ggtcttttcc gagagtaaaa gaccgcgagt
      541 ttcgatgccg gccgctttgg ccagaagcgt caggtagccg ttttggctgt aaaggtttgt
      601 gaaaaccagc gccaccacca cgagtgaagt gattgagggg atgaaaaatc cggcccggaa
      661 aagtccgcgc cagggcagtt tctgattcaa cgccaacgcg aaggcgagcg ctaaaaccgt
      721 cgttaccggg acggtgccga tgacgaagat gaacgtattt ttcaacgcta tccagaaggc
      781 cggatcgcgg aataacttct ggtagttggc gaggcctatc cagtcggatt gcggattgag
      841 aatgccgtaa ttggtgaaac tcaaataaag tgaccaaacc agggggaaaa gccagaacaa
      901 aagaagcgtc agaatccagg gagaagctaa gaggtagggc tggcttcggg cccgcacgtc
      961 acctcacgga gatttcaaaa gcgcgtcaat tgtcgcgctc gcgtcgacta aggcctgccg
     1021 gggtgacttt ttgttgtaca tcgcttcttc caccgccttt tcaatcgcct cctcgatgta
     1081 gacccatttc ggatggaaag ggggaaagac agagaattgc atctgctcga tgaaagtttt
     1141 ccggttgggg tcgctctgga aataaggatc ggcagcggca tcacggttcg ccgggttgga
     1201 aagcccggct ttttgacaga attccagatt gatttccctg gcgatcaaaa agcggaggaa
     1261 agcgagagcc gcggagagat ttttggtttt ggcgtttacc gctaggtact ccccgccggc
     1321 gaatgaggcg ggcgggccgg aatcactccg cggtcgcggc agcaacgccg ttccgaattc
     1381 aaacgccgga ggagttttgg tcaaacgctt caagagccag tcgccggaga tgacgaaacc
     1441 caatcttccc gcgcagaagg tatcgtctaa ttcagtctgc gttgccatga gcccggcgtc
     1501 acagagtttg gtgtagaact ccagcgccgc cacgcttcgc ggctcgtcca ggagcgagcg
     1561 ggattggtcg tccgcaagga tttgcccgcc gttggcccaa aggaaaggga ggaatttttt
     1621 atagagtcgg tggcgctcgt tggcgttggc gccgaaaccg taaatctggc tccccagagc
     1681 gttaatagtt ctggcctgca gcaggagttc gctccagctt tgcggcggac cggtgaatcc
     1741 ggccttttgg agcagggtgc gattgtagaa cagcacccgc gttcccaaag tccagggcac
     1801 gccgtaaagc cgctcgttac gcgtcaccgg ctcccagcgc agcagttgct ctttcagatc
     1861 ggcgaccgaa tcggtgaggt catacaactt tccagcggtg ataaattcgg ggacccaatc
     1921 ggaaccgaat tcaataacgt ccggcgccgc atcggcgccg aaggcgatgg cgattttgtc
     1981 gtgccccgcc tcccaggtga ggtcggtgac ggcgactttg attttggggt agcgtttttc
     2041 aaatttctgt accaaactgt caatgagcgg ccgggtttcc cggtcggtcc ggaactgcca
     2101 ccaggtaagg gtgaccggct cagcttgctg ccgtccgcag gcggacatca gaactgtcgc
     2161 atagccagcg acaacgaaaa cgagccagaa acggaatttc atctgcatcg gtgctccggg
     2221 tagagattct attggctcag gtatcggctg tcaacgggaa aatatttcgt tgacttaatc
     2281 ccagccgccg cttaagtttt ctctcggtca ggaggaggga gagccgtact gcgtttgggc
     2341 aaaaggtcca ttcaccggcg ctggtcaagc gtcaacttct ttttcctctt ttttcttgca
     2401 tttactctgg ctggagaacc ggccgggtcg gctgtttccg agtcaatggg ctttcaaact
     2461 gaccgcctgc ctattctcgt cggttttctc gtctgcgctg ccgttttgct tttgacgctg
     2521 actctgggga ggaagcggaa gggatatgtc cggcccattc cggggctcgc ggcggtggac
     2581 gaagcggtgg ggcgggcgac cgaaatgggg cggccgcttc tgttcacctc cggcaccggg
     2641 gaagtggacc ggatggcaac cttagcggcg ctttcgattc tgggcacagt ggccaaaacc
     2701 gcggccgagc atgatacccg cctgatttat cccaataacg acccggtggt ggcggcggta
     2761 gcgcaggaag tcgtaaaaga aagttgtctc cgggccggcc ggccggaggc gttccaaagc
     2821 gaggatatct ttttcgtgac caaaagccag ttcggctacg cggcggcggt ggacggcatc
     2881 atggtgcgcc aaaagccggc cgcctgcctc ttcttcggca cctttgaagg ggaatcgctc
     2941 attttcgccg agaccggcaa cggcatcggc gcaattcaaa tcgccggcag cgattccacc
     3001 atccagctgg cctttttcct ggtcgcctgc gactacaccc tgatcgggga agagttgttc
     3061 gccgcttccg gctacctctc cggtgatccg gtgatcgtcg ccagcctgcg cgggcaggat
     3121 tacctcaaat tggcggtgat cggtttcctt cttttgggag cgctcgccgc cacgttgcac
     3181 attgccgacc tcgctaaact tctggggggc ggatgagccg ccggctgccg ctctggattg
     3241 cgttcgggtt tggcgtgctt ttgctcgtcc agtacttctt ccggcacccg gttgccaccg
     3301 gcttttaccg gaacaccctg gactggatgc aggtagtggc ggtcttttcg ctcatcgtcg
     3361 gtgccactgc tttagccgga atgcatctga aagcagtggc ggcgcggagc cggggctggg
     3421 gatacaatct ggtgactttg gccggtctgg gcgggatgat tttctgcggg gcgctctggg
     3481 gcatcgatga gcattcacct ttcacctggg cttttctgaa cctccaggta cccttgcaat
     3541 cgacgatgtt ttcccttttg gcgttcttcg tcgcttccgc cgcttaccgc ggtttccggg
     3601 cacgttcttg gccagcggca gtcctcttgc tcgccgcagc gattgtcatt atgggtcggg
     3661 tgccggtagg tgcccaaatt ttggctggtc tgccgcaggc agccgactgg gtgctgaacg
     3721 ttccctcgat ggcggcccgg cgggggattt tgatcggcat cggcctggga gccatcgcca
     3781 cctccctgcg tgtcatctta ggaatcgagc gaacctatct gggaaaggaa gggtgatatg
     3841 actttccttg tccttctatt ggtgattgta aatcttgcgc ttctctacct ctacctcaag
     3901 aagcgcggca ctccccgatg ggagcgggag aaaagtgaaa ttcagcaggt catgaaagaa
     3961 atcacgcagg ccctgcgggc caaggaactg aaggcgatca gtcgctacct ggccgacgac
     4021 ttcaccgcct tcctcgattc gccctaccgc tgtgacagcc gcaaggcgta tctcgcagaa
     4081 ctctccgcgc ttttcgaaca cggcggcatt gaagagcgca gtttcaggca ggaaaaggtg
     4141 caagtgatta atgaaaactg cgcggtggtg acctatcatt ttaccgaaaa agggacgcgt
     4201 aaagacaaac tgtacgagcg gcagggtaag gcaaccgagg tattccgccg ccagccggaa
     4261 gggtgggtgg cggttcactt ccaccacacg ttagtggatg aaaccaaaaa gcccttccac
     4321 tttccgatgg ccgagcccgg cgtatgacct ttttccgctt cgaccgccgc tacctcttcg
     4381 ggtttatggc tctggcggtg gcggtgcctt tgctttttcc tctgcacctg ccgaacgagg
     4441 taacgccgga cgtccggcat ttctacaccc ttattgattc gcttccggcc ggaagcgtag
     4501 tcttagtctc gttcgaccac gaagcttcca ccattgtcga agtgcggccg ccagccactg
     4561 cggtcatccg ccatctgttt caaaaggata tcaaaataat tggggtatcg cttctggctg
     4621 aaggcacggg catcgggcgg gagctcttac tccggctggg aagggaatat ggaaaacaag
     4681 agggcactga ttttgccttc ctcggcttcc ggccgcagta tgcggcgacg atttctggct
     4741 taggagagga tatccgtcgg gtctttccca ccgattaccg ggatcgtccg ctggattcgc
     4801 tcccgttatt ttcgagcgtg aggaatctcg ccgatctggc gctggtcatt tcggtggccg
     4861 acggcgacct gcccgccacc tgggtcgatt atgccgtcgc ccgctatcac cagaaggtgg
     4921 ccgccgcggt caccgccgtg atggcgacca cctactaccc ctacctcaac tccggccagt
     4981 tagtcgggct cgtcagcggg ctcaaagggg cgagcgagta tgaactcctg ctgcataaac
     5041 ccggcggcgg ggtgcgcggg atggacgcac aatcggtggc ccacctggct ctgctcctct
     5101 tgattggcct ggggaatctg gccttcctcg ccagtcgaaa aagggcggcc cggtgaacgt
     5161 ggagacactg atcgccgcaa ttcttactct ggccattttt tcttttctgt accgggataa
     5221 tcctctctac aaatttgccg agcacctctt agtcggcatt tcggtcggct attcggtcat
     5281 ggtggcggtt ttttcgacca tcgggccaaa gctgttttcg cccctcgggc accgccccac
     5341 cccggccgct ttcgtttcgc tcctcttagc ccttttcctt ctggcccggc tggtgccgcg
     5401 ctgggcgtgg gtctcccggc tggcgctttc gcttatgatc ggggcgggcg ccgggctcgc
     5461 cattccggcc atgctccagg cccgcattct ctcccagctc caggcctcgc ttttgccctt
     5521 gacctccctc aacaacattt tgatcgtcgt gggcatcgtc accaccctcc tatactttta
     5581 cttttcccgc gagcagaagg gagtctggaa agggacggca gtggtgggca cctactttct
     5641 catggttttc ttcggcgcca ccttcggcaa cacggtgatg tcgcgcattt cgctattgat
     5701 cggccggctg gaatttctct taggcgactt cttgggtctg attcgatgag gaaatgtttc
     5761 gggcgtgtag caacggtctc ctgaccgtcg agatgaaact gagcatgacc tgtccccggt
     5821 gcggcgccgc catgcgcgaa gagaaacgct tgttccacaa acagcgcaag tgggtctgcc
     5881 cgcgctgcgg gaaaatccgc tttcaaaaaa gagggaaaac agagtgaagc cgcgccgcca
     5941 ggggctgccg cccctgtgca tccgcctaac ggttgccgaa gaaaaaagaa ggatcgctgc
     6001 gccggggatt ctggcgcgct gacttttatg atcttgcgaa ctttcatcct taaggtcgcc
     6061 ggctctgtcc tggccggact tttgcttttc ccggagccgg ggcgcgcgca ggcagcgcag
     6121 agcgattctg cccctgccag ccgcaccgct ccgaccgcaa acccggctcc gccgaaaaag
     6181 aagccggcca agcggcggcg tccggtgcgt cccaaaaaac cagcgccgga gctgtcactc
     6241 acggcccggg cggcggcggt ggtggatgac cggaccggcc tggtgctcta tgaaaaaaat
     6301 ctcacctgtg tccgccccat cgccagcctc accaagctgg tcaccgcttt ggttttcctg
     6361 gaggccgacc cgaatctcga ctcggtgatc acgatggaga aagccgaccg cgcccggcgg
     6421 ggacgctcct ccctggcggt gggcgagtcg ttcgccctgg gcgacatcct ctacgcctgc
     6481 ctcatggcgt cggacaaccg ggccaccaaa gcgctggtac gctcaagcgg actggggccg
     6541 gatgatttca tccaccgcat gaacgaagta gccagggaag tgggcatgga ctcggcccgc
     6601 ttctttgagg tgaccgggct ggacaaccgc aatgtggcca ctgccctgga ttgcatccgg
     6661 ctggtgcatg ccgcgctgga aaacccactc atcgctcgca tcaccgccac ggagcgccac
     6721 acgttctact ccgccaactg gcaccggagc catacgattt tcactaccaa ccggctggtg
     6781 cgccagaagt gggacgtagt gggggggaag accggtttca tcggccaggc ggggtactgt
     6841 ctggtgacgc gggtgcgcaa cagcgaccgg caggaggtca ccgcctgcat tctgggttcg
     6901 cgctccaaca accgccgttt tgccgatgcg cgcaagctgg tgcagtgggc cttcaaaaaa
     6961 ctggcggcac cagaagcgcc ggcagcggat acgccgtaag aacaaagctt ttccgagaga
     7021 gcgagcgaag cggttgcagc gtctagcgct tccctgaacg ccggtgacga tcgcttttcc
     7081 cgggggacgc gcgatcgttg aaccaggagg ggttggtacc agtgccgcag gccagaatcg
     7141 aactggcgac acacggattt tcagtccgtt gctctaccga ctgagctacc gcggcagata
     7201 tcgaacaaat tcggtctaaa cattctagaa aaaagattct caaagtcaag ggagaaaatg
     7261 accccccctc tccggtggtt tcatacagct gaccctgggg ctctgcgcgg agtaacaagt
     7321 tgcgccggca agtcttacct atcgttccgc gcctgtcttc ttctggcacg caccttgccg
     7381 aatggggggt gacggatgaa aagaggagaa attcagttga cagcttctcc agagcgaccg
     7441 tatatttcaa tgtgaaagaa ttctcaaagc ccttatggga ataacgctca agatcgggac
     7501 gttcgtcgcc atgacggcgg ttatcattct ggcgttcgtt ttaccggcgc cgcaggccaa
     7561 aatcggtgag gcctcgcgga ttttcttctt ccacgtcccg gcagcgatcg tcgcttcgct
     7621 cgcttttttg atggcgatgg cgtacagcat cggctacctc aaatcccgat ccgtgcagta
     7681 tgacctgaag gcggcgacga cggcggaact ggggcttttg ttttccatcg tggcgaccgt
     7741 ttccggcgcg attttcgcca aggtgacctg ggggacctac tggaactggg accctcgcga
     7801 aacctcgatg ttcgttctga tactcattta cggcgcctac ctgacgctcc gctccgctat
     7861 cgacgccccg gaaaggcggg cggcgctctc cgcggtctct gcgattttag ccgtggcccc
     7921 agccatgttt ttgattttcg tcgtcccgcg ggtttaccgg tcgctccacc cgactgatac
     7981 cattgtgaac gtcaaaggcg aatcggggat gtcgccggaa gtgcgggcgg tcttttacct
     8041 ctccatcgcc accttcgtcc ttttatactt ttgggttttc aggctctcga cccgtatcaa
     8101 aatgagaacc ttacggagtg aggcggcggc atgacttcga actacacggc tttggtggtg
     8161 acgctcgtaa cctggctggg tctttttttc tacctctggc ggctggaccg ccgggtaaaa
     8221 cggtgggagg ataaatgaaa cccaagtacg tctttggcat agtccttttg gcggccttta
     8281 tcgtcttcgg tgccatcagt ttcaaaaagt ccctaactcc ctacgtgact tttgccgaag
     8341 ccatgaaagc ccgagggagc gtgcaggtga tcggccaact ggtgaaagag aagaccgcgt
     8401 acgataccac cgcacaggtg ttgcgcttca cgctcgccga cgatcacggc cacacccttc
     8461 ccgtgaccta ccacgggatt aagccgggga actttgagca ggcgatttcc atcgtggcca
     8521 tcggccgctg cgagggagag gccttcgacg ccgaccaact cctggtaaag tgtccctcca
     8581 agtaccaggg agaagaggtc aagaaatatg atgctcggta atttcctctt agcgctcgcc
     8641 accattgcct gtgctttttc tctggtcggc tacttgctga gcctgcgcgg ggggcggggc
     8701 gtactcgccc tggcgcgggg cagctactac gttttggcgg ccagcaccac tttggctctg
     8761 ggtaccttgg tttacctctt tatgacccac cgcttcgacg tggcctacgt cgccagttac
     8821 agctccaaag acctgccctg gtattttgag ttttcctcgc tctgggctgg acaacaggga
     8881 accttccttt tgtggctttt ctggtcatcg ctagtgggtg ttattctttt gcgtacgacc
     8941 aaagaaaccg aatcctgggt gatgaccttc tacctgggga tacaagtctt ctttataacc
     9001 atcctgctga agcgctcgcc ttttcttacc catgcggcgc cgccggccga cggccaggga
     9061 ttaaatccac tcctccagga ctactggatg acgatccacc cgccggtgat gtttttgggt
     9121 ttcgctttga cggccgtgcc cgcggcctac gccatggcgg ctttggcctc ccgcaactac
     9181 gaccgctggg tcgagcgggc gcggccctgg gccaccacca ccctcgccat cctgggcacc
     9241 gccatcatca tgggcggcta ctgggcctat aagaccttgg gctggggcgg ctactggggg
     9301 tgggacccgg tggagaatgc ctcgctcatg ccgtggctcg ccaccatcgc tttagtgcat
     9361 ggttttatgc tccagaaaca gagaagcgcc ctgcgccgga ccaatctgac cctggcgctc
     9421 tcggccttcc ttttagtggt ctacggtacg tttttgaccc gcagcggagt tctggctgat
     9481 ttctccgtcc attccttcgt cgatttaggc atcaacggct ttctggttat tttcctggga
     9541 gtcttcgccg tcggcgccgc cggacttctc ctctggcgca gccggcagat caaaagcgag
     9601 cgcctggtca aaaacctttt ggctaaagaa ttttccgtgt tcctctcact ggtacttttg
     9661 gtcgtcacga cgatcatcgt gcttctgggc acctccgcgc cacttctgac ccgcatctgg
     9721 ggccagccgg gcaacgtcaa caccgtctac tacgtgcgga ccagtttgcc catcggcatt
     9781 cttttggccg tggtcttagg cgtcatgccg taccttttat ggcggggttc ggggctccag
     9841 gttctcgccc tgcgggcgct ctggcccgcg gtcgccgggc tagccattgt cggcctttcc
     9901 atccttctgg ggtttcgttc gcctttgtac atcctcttcg ggttttttgc cgtctttgcg
     9961 ctcctgtcga actttttagt tttctaccgt acgttccagc tcaagccggc ctacgtgggc
    10021 ggacatctga cccacgtggg ggtggccttt atgctcctgg gcctcattac ctcaaacgtc
    10081 tactccacct ccgagaaaat caccgtgacc cggggggagt cgaagaaggc gttcgggtac
    10141 cagttcacct accaggggct ggccgggccg gtggaagaaa aagataatgc ggtacatgtg
    10201 ctggtcacgc ggggacaaaa gtcatttacc gccacgccca agtttttctg gagcgaatac
    10261 aaccgcgact tcatgcgcaa accgtacatc aagcgggccg tgttttccga cctctacatc
    10321 tcgcctatgg ctcactcggt gatggatcga gccggacaga acacgctggc cctgaagcag
    10381 ggagaaacca aagaggccta cgggtacaaa atcactttcg agaatttcga agtcctggag
    10441 catgaccagg aaaaaggctt gatcaaagtc ggcgcggtac tgagcgtggc cccctacggc
    10501 cgcaaggcgg tcgtcggcag ccccggcccc cagcgtcggg aaccggtttt tgttctggtc
    10561 ggggaggagc agagtttgat tcccgacacg ctgccgggcg gtgagctcat ctacctggcc
    10621 gcgccgaaac cggagaccaa ggaggtcgta ttgggtttca gcgggttgaa ccaagcccag
    10681 gccgcagcgg ccgactacgt ggtgctcgac atttcgcgca aaccgctcat ccacctcctc
    10741 tggatcggca ccacctgcgt cgctctgggt gcgctcacgg cgtcgtaccg ctggggtcgg
    10801 cgcaggatgg ggacgaaaga gctgagcgta cgccaggtcg gtcaggcgcc acccctatcc
    10861 gcagaagtta aagagccaac cctggcaacc agagtataga cattctttta tcgaaaaaga
    10921 aagtgaagcg cagggaaaga acccgcgctt ctttttttgc attacccctg ccgagccccg
    10981 acgctcttcc tttttgggtg agggtggttt gatgtcggct gactctggag agcaggcgtt
    11041 agcgccgcac ctcacccccg gactgacgcg cgaacgcccg ctccggtctc ccggtttgcc
    11101 tttccgccgc ctcttttgta taattgggaa cgcgaggcat gaatctggaa atcgcaaaaa
    11161 aatctgcacg gtagcttcgg aggctttccg ggaagtcgag tcggcgtcgg tctgctttgt
    11221 gtggcgcgct ttgcgccgcc cgggtgcaac ctacgatttt ggtcggattg tgccgattca
    11281 tagagagtag agaagaccgc ttctcagtct ggtaaaacgt acgacactgc catgcccgat
    11341 tttgaatcac cgcttgagca cttggaatcg gttgatcggc tgcggagcac gattgaagcc
    11401 aaaatctccg aactgtccga ggccaacaag ctccacaaac gcaagatctt tgatctctac
    11461 accgtttttg aaattacccg gcacctgaat acggttttag accgtgacat tctgttcgac
    11521 gggattcttt tggcctgcct gggccaaatg ggggtccagg gagcggccat tgccgtgccc
    11581 gacccggaaa acttccgcat cgtcaagacc aaagggacct ttgcgccgga cgggaagccg
    11641 ctccagttcg ccccgacggg cgcttttgtc cgcctgttca ccgacgagcc ggccccgttg
    11701 accgtcctcc agctgcaaaa ccgtctgccc ggcttcccgg aggtgagcgc cctggaacgc
    11761 gcgggagtcg ctctggtggc gccgatgctg gtcaaggccc acttggaagg gctcctgctt
    11821 ttgacgaata aactttccgg acatcacttc accgaaggcg atctagagtt tttggcgatt
    11881 ctggccaacc agctggcggt ggccgtggag aatgcccggc tttttgcgag cgagaaacag
    11941 gcgtaccaga aactgcggga aatgcagggc cagttggtcc aggcggagaa gttcgccgcc
    12001 atcggtgaac tggcggcttc cattgcgcac gaagtgaata atcccctggg gatcatcaag
    12061 aactacttgg tgcttctggc gcgcgcagtg cccggcggcg aggcggccca ggagtacgtt
    12121 gccatcgtcc aggaagaagt ggaccggatt gcgcggatcg tccggcagtt tttggatctc
    12181 taccggcccc aggccgaaca gatggtcgcg gtcgacgtcg gcaccattat cgatgagacc
    12241 ctgatcttcg tcggcggcca actgaaaaac cagaatattc gggtgaaccg gctggcgaac
    12301 ggcccggtgc cgaagatcgt gggttccggt gagcaattga aacaggtctt cctgaacctt
    12361 ttgatgaatg ccaaagactt catgccgacg ggcggacaaa tcgatatcaa tgtcggtgcc
    12421 ggcaacggcc gggtgaaagt ggaaatcgcc gacaccggct gcggcattcc ggaggagact
    12481 ttggccaaaa ttttcgagcc gttcttcacc accaaaaagg acaataacgg caccggtctg
    12541 ggactttcgg tctgctacgg tattgtcacc cgccaccacg gcaccatccg ggccaaaaat
    12601 agggtacccc atggtgcctg cttcactctg gaatttcccg tggcccagga gtagccggct
    12661 gggtcatgtt ggcgaatcag tcggtaccgg cggtagactc tgacgcggca atgaacgtgg
    12721 gtgacccgat tcccgagctt cgcttccccg gtgaccctgc ctgcgtgccg gcctgtatcc
    12781 tgatcgtcga cgatgaggag cggatgtgtg ccagtctggc ggcgctcctg agcagggccg
    12841 gctacaatgt ggaggcaacc gccgaggcag aaggcgccct ccagtggctc aaaacgcgca
    12901 cctacgacct ggtgatcgcc gacatcaaaa tgccgggggt ggacggattg gaactcctgc
    12961 gggtcgcccg gcaggccgat ccggacgcct tggtcatcct catgaccggc tacggatcgc
    13021 tcgattcggc cgtggccgcc atcgggagcg gtgcttatga ctacctcttg aaaccgatcg
    13081 agtttcccgc attggagttg gtggtcaagc gggctttcga aaagcgccgc gccgacctgt
    13141 cgcgccgggc ccttttagcc gagctccagg cgaaaaacga actgctctcc cgtaagttcg
    13201 cccagctgga tgccctctac caggcggccc ggtcgctggc ggtggcccgc gatttagacg
    13261 agctgttggt caaaatcgtg acgctcgcga cggaggtgat cggtgcccgg gtgggttcga
    13321 taatgctggt ggagccggag acgcaggggt tggtcgtcaa agccgccctg gggctggaac
    13381 ccgaaatcgt ccgcactacc cggctccctt tcggctcttc catcgccggc tacgtggcgc
    13441 aaaggagcga accggtggtg gtggccgacg tggaaaccga cggccgcttt gcccgcgtgg
    13501 ccaaagcgaa gtacgaaacg atgtcacttc tgtgtgctcc tctggtcgtc aaacaagagg
    13561 tgttgggcgt gatcaacctc gcggacaaac gggggggcgg ggtcttcggt ccggacgacc
    13621 tgcagcttct ggtcaccttt gcctcgcagg tggcggtggc catcgacgac gcccgccaga
    13681 tagagcaggc gctccagaac gcccggcgcc tggtcaccat ggggagtctt ctgtcggaga
    13741 tctcccatga cctgaaagcg cccttgacca atatccgcgg cagcctccag attctccggg
    13801 agaagctgac gcgttccccc gaagccgccg agatcctgaa agcgacggaa agagaggtct
    13861 ttcgcttgac cgaactggtc aaggaactgg tcgatttctc caatcccaac aagtaccacc
    13921 tccagcgagc cgacctctcg ggcgtcatcc gccgcgcctt aaagctcgta gaaagcgacc
    13981 tggccaaaaa gaagatcgat ctccagttgc gggtgaccgc cgacctgccg cctattttct
    14041 taaacgaaaa tgagcttatc gagatgcttt tgaacctggt caccaatgcg ttcgagagca
    14101 tgcccgaggg cggcttgctg gaagtgggcg cgcagggaga gcagggcggc gtgcgcctgt
    14161 gggtcaaaga cagcgggggg ggtatactcc cggagcggct gccccgaatt tttgagcgct
    14221 actataccac caagccgacc ggcaccggac tgggactgcc gattgtcgaa cggatcgtca
    14281 aagcccataa cggcaccatt gccgtcgaaa gtacgccggg gacgggaacg acctttgcgg
    14341 tcacctttcc ggccgccgag cgttagccac gtggggaggc aggccggcgt gccgatagca
    14401 ataaggtagc agcggctgtg cggacaaacg cgat
//
LOCUS       DBNGZA010000338         3724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2079420, whole genome shotgun sequence.
ACCESSION   DBNGZA010000338 DBNGZA010000000
VERSION     DBNGZA010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2079420"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16FU_07495"
     CDS             <1..186
                     /locus_tag="AC16FU_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994248.1"
                     /translation="TTEFKQKSPRPLRGGLRIEKAQKLLATRLLSVPEQLEILQGQLS
                     ATRTSPAPPEELRQVKK"
     gene            333..1511
                     /locus_tag="AC16FU_07500"
     CDS             333..1511
                     /locus_tag="AC16FU_07500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR2994249.1"
                     /translation="MTEKIAQVQSYLKAQDLSGWLLYDFRRMNPIAVEFLGITGIQTR
                     RWFYFIPAQGTPTAIIHKIEAHNFDSIPGNKVQFAGWKELETALQTVLKGAKIVAMEY
                     SPRNAIPYISRVDAGTIELVRSLGVEVVSSADLVQYFQARLTDEQYQTHLAAAKALME
                     IKDSTFALAAEKVKTGQPFSEYDLQQYMWQLYQRYGLVADAPPIVAVNANAGNPHYEP
                     TLEKHAPIQKGDLLLIDLWAKQKAPGAIYGDITWVAYLGDRVPEKFAKIFAIVRDARD
                     RAIQFVQEQVKKGVPLYGWQVDDVSRGHIASQGYGDYFTHRTGHSIGTEDHGNGVNID
                     NFETRDQRQIIPGVCFSIEPGIYLPEFGLRSEIDMFVSAGEGRVTTLPLQTEILPLLK
                     "
     gene            1515..2384
                     /locus_tag="AC16FU_07505"
     CDS             1515..2384
                     /locus_tag="AC16FU_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR2994250.1"
                     /translation="MHRPAVIFALTGNLLISCGTFLVAKYALSEIAPLHLAFLRFLLA
                     SGFLYAIHRFVIKGQKLAKGDRWKILFLGFIAIPINQGFFLWGLSHSTPTHSALFYST
                     TPAFVLLFALFLAQEKVTLRKTLGIGLAVVGVIIVLLEHGLVFDRRYLFGDFLLVIAT
                     MAWALYTVLGKSYLQRYGAISLTTFAMLSGTLIFSPLGLYGISSFDYHTVTVKGWLAL
                     FYLSFVTSVVAYSLWYYALARIEATKVAVVNNFQPVITALFSAWLFGEQFSTHFILGG
                     AVVLAGVFVVELA"
     gene            2381..2836
                     /locus_tag="AC16FU_07510"
     CDS             2381..2836
                     /locus_tag="AC16FU_07510"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR2994251.1"
                     /translation="MTRSKIKKGSPKISTAERARQVLKVVKQVPVLHYLGIKPVRLGR
                     GTSTFAVRFTQRLAQVQGILHGGILATLADTAATFASHMLLVSPQETITIELKINYLR
                     PVGSGRAIARARVLHCGQRTTVTVVDVKNQKGQLCATVLVTNLIIVRRI"
     gene            3042..3530
                     /locus_tag="AC16FU_07515"
     CDS             3042..3530
                     /locus_tag="AC16FU_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2994252.1"
                     /translation="MTEKEMFLQTWEREFPTTLKVLKAFPPNKGDFKPHERSLSAKQL
                     GWIFVQAQVGIDQVLKGTFTLPPQFPPVPDKWEEVLSTYEKSHKGIVDQLRKAAEPDL
                     NKTFQFLLGPGKMGEIPKMQFLWFVLSDNIHHRGQLSVYVRMAGGKVPSIYGPSADEP
                     WF"
     gene            3573..>3724
                     /locus_tag="AC16FU_07520"
     CDS             3573..>3724
                     /locus_tag="AC16FU_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR2994253.1"
                     /translation="MTASSLDKTFSALSDPTRRAILRRLANGELTVTELAEPFDISLP
                     AVSKHLR"
BASE COUNT          806 a         1047 c         1014 g          857 t
ORIGIN      
        1 acgacagagt tcaagcagaa gtcaccgcga ccgttgcggg gcgggttgcg catcgagaag
       61 gcgcagaaac ttttggcgac ccggttgctc tcggtgccgg agcagttgga aatactccag
      121 ggacaactct ccgctaccag gacaagccca gccccacccg aagagctgcg gcaggtaaaa
      181 aaatgacgac cggaaaaggg aagttgcttt cccgtaagat tgacgcaagt tgaaggcggg
      241 aggttcaaag catgcgcaaa ttttggctcg caggacccgt ggtactattt tgcctgactg
      301 tttctatgca agccgaagag aaggggaaaa cggtgacgga gaaaattgcc caggtgcaga
      361 gttacttgaa agcccaagat ctttccggct ggcttttgta cgatttccgg agaatgaatc
      421 cgatcgcggt ggaatttttg ggcattaccg gcatccagac ccggcgctgg ttctacttca
      481 ttccggctca ggggacgccc acggccatca tccacaaaat agaagcgcac aacttcgaca
      541 gcatcccggg caacaaggtt cagttcgccg gctggaaaga actggagacc gccttgcaaa
      601 ctgtcctcaa gggtgcaaag atagtcgcta tggaatattc cccgcgcaat gccatcccct
      661 acatttcccg ggtggatgcg gggacgattg aactcgtccg cagtctgggg gtggaggtgg
      721 tttcttccgc cgatctggtg cagtatttcc aggcccggct gactgacgag cagtaccaga
      781 cgcatttggc tgcggcgaag gctttaatgg aaatcaaaga ttcgactttt gcactggcgg
      841 cggagaaagt caagactggc cagccgttca gcgagtacga tctccagcag tatatgtggc
      901 aactgtacca gcgttacgga ttggtagcgg atgcgccgcc gattgtggcg gtgaacgcca
      961 atgcgggtaa tccccactac gagccgacgc tggagaagca cgcgccgatt caaaaagggg
     1021 acttgctctt gatcgacctc tgggccaaac agaaggcgcc gggagccatc tacggcgaca
     1081 tcacctgggt cgcgtatctg ggtgaccggg tgccagagaa attcgccaaa atatttgcta
     1141 tcgtccgtga cgcccgcgac cgggcgatcc agttcgttca agagcaggtc aaaaaaggcg
     1201 tcccgctcta cggctggcag gtggatgacg tctcccgcgg ccacatagcg tcccagggtt
     1261 acggcgatta tttcacgcac cgcacaggcc actccatcgg caccgaagat cacggcaacg
     1321 gggtcaacat cgataatttc gagacccgcg atcaaaggca gattattccc ggggtctgct
     1381 tttccatcga gccgggcata tacctgccgg agttcggcct ccgctcggag attgacatgt
     1441 tcgtgagtgc aggcgagggc cgggtcacca cattgccgct tcaaaccgaa attcttcccc
     1501 tcctgaaata gccggtgcac cgccccgccg tcatctttgc cctcaccgga aatctcctca
     1561 tctcctgcgg cacctttctc gtggccaagt acgcgctgtc ggaaattgcg ccgctgcacc
     1621 tggccttttt gcgcttcctt ctggcttccg ggttcctcta cgccattcac cgttttgtca
     1681 ttaagggtca gaagttagcc aaaggtgacc gctggaaaat tctctttttg gggttcattg
     1741 ccattccgat caatcaggga tttttcctct ggggactttc ccactccacg ccgactcact
     1801 cggcgctctt ctacagcacc acgccggcct tcgtgcttct gttcgcgctc ttcttggccc
     1861 aggagaaggt caccctccgc aaaactttgg gcatcgggct ggccgtggtc ggcgtcatca
     1921 tcgttctcct ggaacacggg ttggtctttg accgccgcta cctttttggg gattttcttc
     1981 tcgtgatagc caccatggcc tgggcgctct acacggtgtt ggggaagtca tacctccagc
     2041 gctacggtgc catttcattg actacgttcg ccatgctctc cggcacgctc atcttttcgc
     2101 ccctgggact ttacggaatt tccagtttcg actatcacac ggtgacggtc aaaggctggc
     2161 ttgcgctttt ctacctttcc tttgtaacat cggtcgtggc ctattccctc tggtactacg
     2221 ccttggcgcg gattgaggcc accaaggtgg cggtggtgaa caacttccaa ccggtgatta
     2281 ccgctttatt ttccgcctgg ctctttggcg agcagttctc cacccatttt attctgggag
     2341 gagcggtggt gctggcgggt gtctttgtgg tggagttggc gtgacgcgat ccaaaatcaa
     2401 aaaaggcagc ccgaagattt cgactgccga gcgggcgcgc caggtgctca aggtcgtcaa
     2461 gcaagtgccg gtgctccact atttgggaat caaaccggtc cgattaggac gcggcacttc
     2521 gacctttgcg gtgcggttca cgcagcgcct ggctcaagtc cagggcattt tgcacggggg
     2581 aattctcgct accctcgccg atacggcggc gacttttgcc tcccacatgc ttttggtctc
     2641 tccacaagaa accatcacca ttgaactgaa gatcaactac ctgcgtcccg tcggttccgg
     2701 tcgcgcgatt gcccgtgcgc gggtgctcca ctgtggccag cgcacgacgg tgaccgtggt
     2761 tgatgtaaaa aatcaaaagg gacaactctg cgctaccgtt ttggtgacga atctgatcat
     2821 cgttcgaaga atctgaaaac ggatttcacc gaccgtttcc ccgaatgggg agctatcctt
     2881 ttgtccaaca agcggttacc actacccggc agccgttctt aaaaatagaa agatttttga
     2941 aattaggact tgacaagtag agtatccgcc tttatactta actacctggt taagtattct
     3001 cgaaaccgca ataacctctc aacgcggaaa ggagccagag tatgaccgaa aaagagatgt
     3061 tcctccaaac ctgggagcgc gagtttccca ccacgctgaa agtgctgaag gcatttccgc
     3121 ccaacaaagg ggatttcaag cctcacgaac gctccctatc cgccaagcaa ctggggtgga
     3181 ttttcgttca ggcacaggtg gggatcgacc aagtcttaaa gggcaccttc acgcttccgc
     3241 cccagtttcc acccgtgccg gacaaatggg aagaggttct gtcgacctat gagaagtcac
     3301 acaagggcat tgtagaccaa ttgcgtaagg cagccgagcc cgatcttaac aaaacttttc
     3361 aatttctgct cgggccgggg aaaatggggg aaattcctaa gatgcagttt ctgtggtttg
     3421 tcctgtccga taacatccat caccggggac agctctccgt ttacgtccgg atggcgggcg
     3481 gcaaagtgcc ttcaatttac ggcccctcgg ccgatgagcc ctggttttag tcgaagtttt
     3541 tccgggagga aggcaagttt gatcggaaga tgatgacagc cagctccctc gataaaacct
     3601 tttcggcttt gtccgatcca acccgccggg cgattttgag gcgactggca aatggagaac
     3661 tcacggtaac tgaactggcc gagccgttcg acatttccct gcccgccgtt tccaagcatc
     3721 ttcg
//
LOCUS       DBNGZA010000339         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2186156, whole genome shotgun sequence.
ACCESSION   DBNGZA010000339 DBNGZA010000000
VERSION     DBNGZA010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2186156"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="AC16FU_07525"
     CDS             complement(<1..1019)
                     /locus_tag="AC16FU_07525"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR2994254.1"
                     /translation="MFKKILIANRGEIAVRVIRACREAGTTAAVVFSDADRTSLAVRL
                     ADEAYRLGPPPVRESYLQMEAILTIAKEIGAEAIHPGYGFLAENPRFAQLVAESGLAF
                     IGPPAAAIRALGDKLEARRLMATSGVPVVPGSPEPVDQQNVEKIAAGIGYPVLLKAAA
                     GGGGKGMRVVQQPGELKSALRAAQSEARTAFGDDRIYVEKFLEKPRHIEVQIIADRFG
                     SVCALGERECTIQRRHQKVVEESPSVFVTPELRAGLFDAARQAAHASGYVNAGTVEFL
                     VDARRNYYFLEVNTRLQVEHPVTEEVTGLDLVRLQLQIASGEKLPLTQAEVRFRTRRW
                     SAASMP"
     gene            complement(1319..>1826)
                     /locus_tag="AC16FU_07530"
     CDS             complement(1319..>1826)
                     /locus_tag="AC16FU_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR2994255.1"
                     /translation="PKATAQVLRDGWLYTGDAGWVDQEGYYYITGRLKNVIVTPGGKN
                     VYPEEVEHHLMQSPFILEVLVHGQKVSGGEEVEATVVPDQEYFADFCSTRGGEATPET
                     MEATLKSEIARICAHLADYKRVKRFQIRLQEFDKTASRKIKRRQITPAAPGVSVPAGV
                     MATRDSSP"
BASE COUNT          346 a          555 c          540 g          385 t
ORIGIN      
        1 ggcatagatg cggcactcca acgccgcgtc cgaaaacgca cctctgcctg agtgagtggg
       61 agtttttctc cggaagcaat ctgcagctgc agacgaacca gatcgagccc ggtcacctct
      121 tcggtcaccg ggtgttccac ctggaggcgc gtgttcactt cgaggaagta gtagttgcgc
      181 cgggcgtcaa ctaagaattc caccgtgccc gcattgacgt agccagaggc gtgcgctgcc
      241 tgacgggcgg cgtcaaaaag tccggcccgc aactctggcg taacgaaaac actgggcgac
      301 tcctcgacca ccttctggtg gcggcgctga atcgtgcact cccgctctcc taaggcgcaa
      361 acgctgccga aacggtcagc gatgatctgc acttcgatgt ggcgcggctt ttcgagaaac
      421 ttttcgacgt agatgcggtc gtcgccgaac gcggtccggg cttccgactg cgccgcccgc
      481 agggcacttt tcagttcacc cggctgctgc acgacgcgca tacctttgcc gccgcccccg
      541 gcggcagctt tcaaaagcac cggatacccg attcctgcgg ctattttctc aacgttctgt
      601 tgatctaccg gttcgggact tccgggcaca accggaacgc cggaggtggc catgagccgc
      661 cgggcttcca gcttatcgcc cagcgcgcga atcgcggccg ccggtgggcc gatgaacgcc
      721 aggccgcttt ctgccaccag ttgggcaaaa cgcggatttt ccgccagaaa cccgtagccg
      781 gggtggatcg cttcggcccc gatttctttg gcgatcgtca ggatcgcttc catctgcagg
      841 tagctttccc gcaccggcgg aggcccgagg cggtaggcct cgtcggccag ccgcaccgcg
      901 agcgaggtac ggtcggcgtc ggagaagacc accgccgccg tcgtccccgc ctcccggcag
      961 gcgcggatga cgcgcaccgc aatttccccg cgattggcaa tgaggatttt cttgaacatt
     1021 actcccttag gtcagcaaaa aaccggcgat tcgcagaaat gtacccgaac tgcggagtgt
     1081 tgtcaaacag cggagcagct ggagattatt ctgtagaaga gactgagagg ggatggtttc
     1141 catttttata agtatataaa ctataaagag atgcggttaa tttatgattc cttactttaa
     1201 gtaagacccc catttccggt gctgccggcg gtagcgacgg cttcagcgac agacgtggtc
     1261 gattgagaat gcgtggtcag gcggtccact ccgacctctg atccagagac cgcctgaact
     1321 acggagaact gtcgcgtgtg gccatgactc cagcggggac ggaaactccc ggcgcggccg
     1381 gcgtgatctg gcggcgcttg attttccggg aggcggtttt gtcaaactcc tgcaggcgaa
     1441 tctgaaatct tttgacccgc ttgtagtcgg ccaggtgggc gcagatgcgc gcaatttccg
     1501 acttcaaggt tgcttccatt gtctccgggg tagcttctcc tccgcgagtg ctgcaaaaat
     1561 cggcaaagta ttcctggtcg ggcaccaccg tcgcttccac ttcttccccg cccgatacct
     1621 tctgcccgtg gaccaaaacc tccagaatga aggggctttg catcaggtgg tgttccactt
     1681 cttccgggta gacattcttg ccgccggggg tgacgatgac gttcttcaac cggccggtga
     1741 tgtagtagta cccttcttga tcgacccagc cggcatcgcc ggtatagagc cagccgtcgc
     1801 gcagtacctg agcggtggcc ttgggg
//
LOCUS       DBNGZA010000340         8526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_278680, whole genome shotgun sequence.
ACCESSION   DBNGZA010000340 DBNGZA010000000
VERSION     DBNGZA010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_278680"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16FU_07535"
     CDS             complement(<1..551)
                     /locus_tag="AC16FU_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR2994256.1"
                     /translation="MIQTFQHMFRIPELRRRVLFTLGLLVIYRLGGHIPSPGIDSAAL
                     GELFRSAGAGTLFGLYDLFVGGAFFKATVFALGLMPYISSSIILQLMGAVVPYFQKLQ
                     KEGEEGRRKLTQYTRYGTVLIAALQALGTAVYLESLRVPGTNAPIVPQPGMLFKFLCM
                     LTFTSGTIFIMWLGEQITEKGIG"
     gene            complement(548..991)
                     /gene="rplO"
                     /locus_tag="AC16FU_07540"
     CDS             complement(548..991)
                     /gene="rplO"
                     /locus_tag="AC16FU_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR2994257.1"
                     /translation="MPILPLNPPRGSTKKRKRIGRGPGSGHGKTATHGHKGQKARSGA
                     KFPAHKEGGQMPLARRLPKRGFTNIFKKTFQVVNLRQLSGFKPQEVITPLVLQNRGLV
                     SAVGRPIKILGDGRLLVPVVIQAHAFSRSAREKITASGGRCELVK"
     gene            complement(997..1194)
                     /gene="rpmD"
                     /locus_tag="AC16FU_07545"
     CDS             complement(997..1194)
                     /gene="rpmD"
                     /locus_tag="AC16FU_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041570711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR2994258.1"
                     /translation="MEKSKLEITLVRGFSGQIQKQRRILRALGLSRRHQKVLHADSPT
                     IRGMLAKVNHLVQVTDRSGAR"
     gene            complement(1197..1706)
                     /gene="rpsE"
                     /locus_tag="AC16FU_07550"
     CDS             complement(1197..1706)
                     /gene="rpsE"
                     /locus_tag="AC16FU_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR2994259.1"
                     /translation="MRTDRQSRDDQDFFEFEEKVIQVNRVAKVVKGGRRFSFSALVAI
                     GDKRGRVGVGLGKAREVADAIRKGHEEARKALFKVPVAGGTLPFEVTSHYVSSSVLLK
                     PASAGTGVIAGGPVRAVMEVAGIQDVLTKSWGSTNPNNVVKATLQALRRLNDFVEQEQ
                     LRKAEEAKA"
     gene            complement(1703..2077)
                     /gene="rplR"
                     /locus_tag="AC16FU_07555"
     CDS             complement(1703..2077)
                     /gene="rplR"
                     /locus_tag="AC16FU_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR2994260.1"
                     /translation="MILEQERKKERALRRRKRVQGKVAGTALRPRLSVYRSLKHVYAQ
                     IVDDVNRQTITAAASTGKKVRALLAKGAKKTEVAKLLGQELARLAKEKGITQVVFDRN
                     RYRYHGRVKAVADGLREGGVKL"
     gene            complement(2187..2723)
                     /gene="rplF"
                     /locus_tag="AC16FU_07560"
     CDS             complement(2187..2723)
                     /gene="rplF"
                     /locus_tag="AC16FU_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR2994261.1"
                     /translation="MSRVGKNPVPIPPAVTVQVAGDTVTVQGPKGTLARRFHPYVSVA
                     VADGQAVVTRKGESQFHRSLHGLTRALVQNMVVGVTQGYERALSIVGVGYKAELSGKR
                     LALALGYSHPILFKPPEGITLAVTDQTRIVVRGIDKELVGEVAAKLRALRPPEPYKGK
                     GIRYADEEVRRKAGKTTA"
     gene            complement(2730..3125)
                     /gene="rpsH"
                     /locus_tag="AC16FU_07565"
     CDS             complement(2730..3125)
                     /gene="rpsH"
                     /locus_tag="AC16FU_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR2994262.1"
                     /translation="MLTDPIADMLNRIRNAQKARLREVVIPGSRLKRAVGLLLVQEGM
                     LKACTEERTPPQSRLRLQLLYLAGQKNAITGLVRFSRPGRRAYLSAADLERRSRQMGV
                     VVLSTSQGVMTDRQARKLGIGGEALCRVW"
     gene            complement(3192..3377)
                     /locus_tag="AC16FU_07570"
     CDS             complement(3192..3377)
                     /locus_tag="AC16FU_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR2994263.1"
                     /translation="MACKAMMEKAGREPKFKVRQHNRCWRCGRPRAFLRKFGICRICF
                     RQLALAGEIPGVTKASW"
     gene            complement(3386..3976)
                     /gene="rplE"
                     /locus_tag="AC16FU_07575"
     CDS             complement(3386..3976)
                     /gene="rplE"
                     /locus_tag="AC16FU_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR2994264.1"
                     /translation="MTARLKERYKKEVLPALLKRFGYGNQLAAPHLEKVVINLGVGDA
                     IGNAKLLDAAVADLTAIAGQKPVVRRAHRSISNFKLRAGAPIGCSVTLRGERMYEFLD
                     RLVNVAIPRIRDFRGVSPDAFDGRGNYTLGLQEQIIFPEIVYDKVLKVRGMNITLVTT
                     ARTDEESFELLRGMGVPFRSSRTASDRSKAATTEAK"
     gene            complement(3979..4305)
                     /gene="rplX"
                     /locus_tag="AC16FU_07580"
     CDS             complement(3979..4305)
                     /gene="rplX"
                     /locus_tag="AC16FU_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR2994265.1"
                     /translation="MMRIKKGDQVLIVAGDTKGKIGKVLSVLPGRKRIVVEGDKLIKR
                     HTRPSAKVAKGGIIERERTIHVSNVMLYCTKCSKPTRPAYKVAATAEKGRSKTLRLCR
                     HCGEII"
     gene            complement(4319..4687)
                     /gene="rplN"
                     /locus_tag="AC16FU_07585"
     CDS             complement(4319..4687)
                     /gene="rplN"
                     /locus_tag="AC16FU_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR2994266.1"
                     /translation="MIQEYSVLNVADNSGARRVMCFRVLGGTRKRYAVIGDTIVVTVK
                     DAIPGGTVKKSDVCRAVVVRTTRQTRRPDGSYIRFSDNAAVLINEQAEPRGTRIFGPV
                     ARELRDRQFMKIISLAPEVL"
     gene            complement(4684..4989)
                     /gene="rpsQ"
                     /locus_tag="AC16FU_07590"
     CDS             complement(4684..4989)
                     /gene="rpsQ"
                     /locus_tag="AC16FU_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR2994267.1"
                     /translation="MKTALPIPSVRTQRKVRIGRVVSTKMQKTIVVKILRQLRHPDYG
                     RVIRRSTKLYARAEANQCQVGDLVKLAETRPLSRLVRWRLLEVLERASAPKVEVKDE"
     gene            complement(4992..5228)
                     /gene="rpmC"
                     /locus_tag="AC16FU_07595"
     CDS             complement(4992..5228)
                     /gene="rpmC"
                     /locus_tag="AC16FU_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR2994268.1"
                     /translation="MNVEQLRELSREELLQKKHDLEEELFNLRLRRVTRELENPLRLR
                     TLRREKARILTLLRETELRVPTRSAAKPQPELKK"
     gene            complement(5225..5653)
                     /gene="rplP"
                     /locus_tag="AC16FU_07600"
     CDS             complement(5225..5653)
                     /gene="rplP"
                     /locus_tag="AC16FU_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR2994269.1"
                     /translation="MLMPKRVKYRKQQRGRRRGLSLRGAEVSFGEYGLKALEPAWITN
                     QQIEAARVALTRFIKRGGKVWIRIFPDKPVTVKPAETRMGKGKGNPEHWVAVVKPGRV
                     LFELEGVTEVTAREALGLAADKLPIKTRFVARTALFGTPK"
     gene            complement(5673..6479)
                     /gene="rpsC"
                     /locus_tag="AC16FU_07605"
     CDS             complement(5673..6479)
                     /gene="rpsC"
                     /locus_tag="AC16FU_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR2994270.1"
                     /translation="MGQKANPIGLRLGVIRTWNSKWFSTRTFASLLKEDLTIRRYTHR
                     RLENAGIANIEIIRAPKKISVDIHTSRPGIVIGRKGAEVDKLKEELQLLTKKDVSINI
                     IEVKQPELSARLVGESIAHQLEGRVSYRRAMKKAIAASMKMGAQGIKILCGGRLAGAE
                     IARSEKYLEGRVPLHTLRADIDYATATAHTTYGTIGVKVWIFRGEVLGKGRFTAEARQ
                     AGRLAEERPEPRPRRGRSERRPGRPDHRRGREGKTQTASPVAGGAAGAAP"
     gene            complement(6491..6928)
                     /gene="rplV"
                     /locus_tag="AC16FU_07610"
     CDS             complement(6491..6928)
                     /gene="rplV"
                     /locus_tag="AC16FU_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR2994271.1"
                     /translation="MEAIARARFQRMSPRKVRRVAALVRGKSVDEAIALLDYVPKAAA
                     VPLQKTIKSAVANALAVEGTAKLKSEDLAIRVVKVDGGPVLKRIRAVGMGRAYRIRKP
                     TCHITVVVAGEPKPVQLRGARRTTALKTPKPKGPDKEAPAARR"
     gene            complement(6934..7272)
                     /gene="rpsS"
                     /locus_tag="AC16FU_07615"
     CDS             complement(6934..7272)
                     /gene="rpsS"
                     /locus_tag="AC16FU_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR2994272.1"
                     /translation="MARSLKKGPYVDAKLMKKILKLNETGVKTVLKTWARRSTIAPEL
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFSPTRTFRGHSGKLAAKEAEGGAAPPPPA
                     PAPGEKAPKK"
     gene            complement(7277..8098)
                     /gene="rplB"
                     /locus_tag="AC16FU_07620"
     CDS             complement(7277..8098)
                     /gene="rplB"
                     /locus_tag="AC16FU_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR2994273.1"
                     /translation="MGIKTYKPFTPSRRWITVSDFAEITRSQPERSLLEPLRKSGGRN
                     NDGHITARYIGGGHRQFYRVLDFKRDKANVPAKVAAIEYDPNRSARIALLHYADGEKR
                     YIIAPLDLAVGDTLVAGPEAEIRPGNAVPLARVPLGTMVHNVELRRGAGGQIARSAGA
                     SVQLVAKEGNYGHLRLPSGEIRLVRLENYATIGQVGNLDHEKISLGKAGRTRWLGYRP
                     RVRGVAKNPVDHPMGGGEGKSSGGRHPCSPWGLKSKGQKTRKHKPSDRYILKRRD"
     gene            complement(8098..8394)
                     /gene="rplW"
                     /locus_tag="AC16FU_07625"
     CDS             complement(8098..8394)
                     /gene="rplW"
                     /locus_tag="AC16FU_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR2994274.1"
                     /translation="MKDARTVILRHLLTEKGTRLREKEHKYFFHVATGANKLEVKRAV
                     ETLFKVNVTGVNTQIVRGKTRRYGFRQRTGTRPNWKKAIVQLKTGQTIEALEKI"
     gene            complement(8391..>8526)
                     /gene="rplD"
                     /locus_tag="AC16FU_07630"
     CDS             complement(8391..>8526)
                     /gene="rplD"
                     /locus_tag="AC16FU_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR2994275.1"
                     /translation="LSCRNIPGVTFKRAAAVNAYDLWIADSVLLTKPALAAVEEVFSG
                     "
BASE COUNT         1442 a         2758 c         2514 g         1812 t
ORIGIN      
        1 ttgccgattc ccttctcggt gatctgttct cccagccaca tgatgaaaat agtaccggag
       61 gtgaaggtga gcatgcagag gaatttgaac aacattcccg gctggggcac gatgggggca
      121 ttggtgccgg ggaccctcag gctctcgaga tagaccgccg tgcccagggc ctgcagcgcc
      181 gcaatgagca ccgtcccgta gcgggtgtac tgggtgagct tccgccttcc ctcttctcct
      241 tctttctgga gtttctggaa gtaggggacg accgctccca tgagctgcag gatgatcgag
      301 gaagaaatgt aaggcataag acccagggcg aagaccgtgg ctttgaagaa cgccccgccg
      361 acaaaaaggt cgtagaggcc gaagagcgtg ccggcgccgg cggagcggaa gagttcgccc
      421 aacgccgccg aatcgatccc gggggagggg atgtggccgc ccagccggta gatgaccaaa
      481 agacccaagg tgaagagcac ccggcgcctg agttccggaa tccggaacat atgttgaaac
      541 gtctggatca cttaacgagt tcgcacctgc cgcccgaagc ggttattttc tcccgggcgc
      601 tccggctgaa cgcgtgggcc tgaatgacca ccggcaccaa aagccgcccg tcccccaaga
      661 tcttaatcgg tcggccaacc gcggaaacca ggccgcggtt ctggagcacc aaaggcgtaa
      721 tgacttcctg gggtttgaaa ccggagagtt gtcttaagtt caccacttgg aaggttttct
      781 taaaaatatt ggtaaagccc cgcttgggga gtcggcgggc cagaggcatc tggccccctt
      841 ctttatgggc cgggaatttt gcgccgctcc gggctttctg ccccttgtgc ccgtgggtgg
      901 cggtcttgcc gtgtccggag cctggtcccc ggccgatgcg ctttcttttt ttggtcgatc
      961 cccggggcgg gttgagtggg agaatcggca tgacggctat cgtgctcccg aacggtcggt
     1021 gacctgcacc aggtggttga ccttcgccag catgccccgg atggtggggg agtcggcatg
     1081 caacactttt tggtgccgtc gggagagtcc caaggcgcgc agaattcgcc tctgcttttg
     1141 gatctggccc gagaagcccc gcaccagggt gatttcgagt ttgctttttt ccatgactac
     1201 gcttttgctt cctcggcttt gcggagttgc tcttgttcca caaaatcgtt caagcggcgc
     1261 aaggcctgca gggtggcctt aacgacattg ttggggttgg tcgaacccca ggacttggtc
     1321 aaaacgtcct ggatgccggc cacctccatc accgcccgca ccggaccgcc cgcaatcacc
     1381 ccggtgccgg cactggccgg cttgagcagc acactggaag agacgtagtg gctcgtgact
     1441 tcgaacggca gggttccgcc ggcgaccgga accttaaaga gcgccttgcg ggcctcttcg
     1501 tgccctttgc ggatggcgtc cgccacctcg cgcgcctttc ccagcccgac gccgacgcgc
     1561 ccccgcttgt caccaatcgc caccagggcg gaaaaggaga agcggcggcc gcctttcacc
     1621 actttggcga cccgattcac ctggatcact ttttcttcaa actcaaaaaa gtcctggtcg
     1681 tcccggcttt gacggtcggt tctcacagtt tcacgccgcc ttcgcgcaat ccgtcggcca
     1741 ccgccttgac ccgtccgtgg tagcggtagc ggttgcggtc aaataccacc tgcgttattc
     1801 ctttctcctt ggccagccgg gccagttcct gcccgagcag tttggccacc tcggtctttt
     1861 tcgctccctt ggccagcagg gcccgcacct ttttaccggt gctggccgcg gcggtgatcg
     1921 tctggcgatt gacgtcgtcg acaatctgag catagacgtg cttcaggctc cggtagaccg
     1981 aaagccgcgg ccggagggcg gtccccgcca ccttcccctg cacgcgcttg cggcgccgga
     2041 gcgcccgttc tttttttcgc tcctgctcca gaatcatcgg ccctacccgc tcactcgccg
     2101 gctattcctg ctttcatccc tcacggtaat agaaccagca caaccagaaa taggtttgac
     2161 acgttctatc atctgcgtcc gaatttctac gccgtggtct tccccgcttt gcggcgcact
     2221 tcttcatccg cgtagcgaat gcctttgcct ttgtacggct ccggcggccg gagggcgcgc
     2281 agcttggccg ccacctcccc caccagttcc ttgtcgattc cgcgcaccac gatccgggtc
     2341 tggtcggtta cggcgagcgt tatcccttcc ggcggcttga ataaaatcgg gtgcgagtac
     2401 cctaaggcaa gcgcaagcct tttgcccgag agctcggctt tgtagccgac cccgacgatg
     2461 gagagggcgc gctcataccc ctgagtgacc cccaccacca tgttctgcac gagcgcgcgg
     2521 gtgaggccgt gcaagcttcg gtggaactgg ctttcccctt tccgcgtcac caccgcctgg
     2581 ccgtcggcga ccgccacgga aacgtaggga tggaaccgcc gtgctaaagt tcccttgggt
     2641 ccttggacgg tgacggtatc tcccgctacc tgcacggtga cggccggcgg aatcggaacc
     2701 ggatttttcc ccacgcgcga catagtgctt taccacaccc ggcagagcgc ttcgccgccg
     2761 atccccaact tgcgcgcttg gcggtcggtc ataaccccct gcgaggtcga gagaactacc
     2821 acacccatct gccgactccg gcgctccagg tcggcggcgg aaagataggc ccgccgcccg
     2881 gggcgggaaa aacgcaccaa accggtaatg gcgttcttct ggccggccaa gtagagcaac
     2941 tgcaaccgga ggcgcgactg cggcggagtg cgctcctccg tgcaggcttt cagcatcccc
     3001 tcctgaacca gaagcaggcc gaccgcgcgc ttgagccggg agccgggaat gaccacttcc
     3061 cgcaagcgcg ctttctgcgc gttccggatt cggttgagca tgtcagcaat cggatcagtt
     3121 aacatcgctt ttacgcatct cctgttccag agattttttc tccgtccgtc acccgcacga
     3181 tcgatcggac ctcaccagct cgccttggtc acgccgggaa tctcccccgc cagggccagc
     3241 tggcggaagc agatgcggca gatgccaaat ttgcgcaaga aagcgcgggg ccgtccgcag
     3301 cgccagcagc ggttgtgctg ccgcaccttg aatttcggtt cccgtccggc tttttccatc
     3361 atcgctttac acgccatcgg tacctctatt tcgcctcggt tgtggccgct tttgaccggt
     3421 ccgacgcggt ccgggaggag cgaaagggca cccccattcc gcgcaaaagt tcaaagcttt
     3481 cctcgtcggt tcgggccgtg gtcacgagcg taatgttcat cccgcgcacc ttcagcactt
     3541 tatcgtagac gatttccggg aagataattt gctcctgcag gcccaaagtg tagttccccc
     3601 gaccatcgaa ggcgtccggc gagacgcccc ggaaatcccg gatccggggg attgccacgt
     3661 tgaccaggcg gtctaaaaat tcgtacatcc gttcgccgcg cagggtaacc gagcagccga
     3721 tgggcgcccc ggctcgcaat ttgaagttcg aaatcgaacg gtgcgcccgg cgcaccaccg
     3781 gcttctgccc ggcgatggcg gtcaaatcgg ccaccgccgc gtccaaaagc tttgcgttgc
     3841 cgatggcatc gcccactccc aggttaatca cgactttttc caagtgcggg gcggccagct
     3901 ggttgccgta gccgaaccgc ttcaaaagcg ccggcagcac ctcttttttg taccgttcct
     3961 tcaagcgcgc cgtcatcgtc agatgatttc tccgcagtgg cggcagagcc ggagagtttt
     4021 cgaccggccc ttctccgcgg tggcggcgac cttgtaagcc gggcgcgtcg gtttggaaca
     4081 cttggtacag tagagcatga cgttggagac gtggatggtc cgttccctct cgatgatgcc
     4141 gcctttggcc actttggccg acggccgggt gtggcgcttg atcagtttgt ccccttccac
     4201 cacgatccgc ttgcgccccg gcaaaaccga gagcaccttg ccgattttcc ctttggtatc
     4261 gccggcgacg atcaacacct ggtcgccttt tttgatccgc atcataggac gttgaccctc
     4321 acagcacttc cggcgccagt gatatgattt tcataaactg gcggtcgcgc aactcccgcg
     4381 ccaccggacc gaaaatccgg gtgccccgcg gctcggcctg ctcgttgatc aaaaccgccg
     4441 cgttgtcgga gaagcggatg tacgaaccgt ccggccgccg ggtctggcgg gtggtgcgca
     4501 ctaccaccgc acggcagacg tcgctctttt tgaccgtccc gcccgggatg gcatctttca
     4561 ccgtcaccac aatggtgtcg ccgatcacgg cgtaccgctt gcgcgtgccg cccagaaccc
     4621 ggaagcacat cacccggcgc gcgccggagt tgtcggccac gttcaagacg gagtactcct
     4681 gaatcactcg tctttcacct caactttggg ggcactggcc cgctccagaa cttccaggag
     4741 ccgccagcgc accagccgcg acaaaggcct ggtctcggct aatttcacca agtcacccac
     4801 ctggcactgg ttggcttccg cgcgggcata cagtttggtc gagcgccgga tgacccggcc
     4861 gtaatccgga tgccgcagct gacgcagaat tttgaccacg atggtcttct gcatcttggt
     4921 ggaaacgacc cggccgatgc gcaccttgcg ctgagttcgg acggacggga tgggcagcgc
     4981 tgttttcata tttacttctt caactcgggc tgcggcttgg ccgcgctccg ggttggcacc
     5041 cgcaattcgg tttcccgcag gagggtgagg atccgtgctt tttcgcggcg cagggttctc
     5101 agccgcaaag gattttccag ttcgcgggtg acccggcgca accggaggtt gaacagttcc
     5161 tcctccaggt cgtgcttctt ctgcaacagt tcttcgcgcg agagttcgcg caattgttcc
     5221 acgttcattt cggtgtcccg aaaagcgcgg tgcgcgccac gaatctggtc ttgatcggca
     5281 atttgtcggc cgccagtccc agcgcttccc gcgccgttac ttctgtaacg ccttccagtt
     5341 cgaacagaac ccgccccggt ttgaccaccg ccacccagtg ctcggggttg cctttcccct
     5401 tccccatccg ggtttcggcc ggtttcaccg tcaccggctt atccggaaaa atccggatcc
     5461 agactttccc gccccgtttg atgaagcggg tcaaagccac ccgcgccgct tcgatctgct
     5521 ggttcgtgat ccaggccggc tccaaggctt tcaacccgta ttcgccgaaa ctgacctcgg
     5581 ccccgcggag cgagagcccg cgccggcggc cgcgctgctg cttgcggtat ttcacccgct
     5641 tcggcattaa catgaccggc gctcccgaac gttcacggcg ctgctccggc agccccgcca
     5701 gcgacggggc ttgcggtttg ggttttgccc tcgcggccac ggcggtggtc cggccgcccc
     5761 ggacgccttt cgctccggcc ccgacgggga cggggttcgg gccgttcctc cgccagccgc
     5821 cccgcctgcc gggcttcggc ggtaaatcgg cccttcccca acacctcacc ccggaaaatc
     5881 cagaccttga cgccgatggt accgtaggtg gtgtgggcgg ttgccgtggc gtagtcgatg
     5941 tcggcccgca gcgtatgcaa aggtacgcgc ccttccaagt acttttcgga acgggcaatt
     6001 tccgcaccgg ccagccggcc gccgcagagg atcttgatcc cctgcgcgcc cattttcatg
     6061 gaggcggcga tcgccttctt catggcgcgg cggtaggaga cccggccttc cagctggtgc
     6121 gcgatgctct cccctaccag ccgggccgac agttccggct gcttgacttc aataatattg
     6181 atggagacat cctttttggt caaaagctgg agttcctctt tcaatttatc aacctcggca
     6241 cctttacggc cgatgacaat tccgggacgg gaagtgtgga tgtctaccga aattttcttc
     6301 ggggcgcgga tgatctcgat attggcgatg ccggcatttt ccagccggcg gtgggtatac
     6361 cgccggatgg tcaaatcttc tttgagcaga gacgcaaagg tgcgcgtgga gaaccatttg
     6421 gagttccagg tccggatcac tcctaaacgc agtccgatgg gattggcttt ctgacccact
     6481 gctttcgctc ctaccttctg gcggccgggg cttccttgtc cggccctttc ggtttcggcg
     6541 ttttcaatgc ggtggtccgc ctggctcctc gcagctggac cggcttcggt tcgccagcca
     6601 cgaccacggt gatatgacag gtcggcttgc gaatccgata ggcgcgcccc atgcccaccg
     6661 cccggatccg cttgagcacc gggccgccat cgactttgac cacccggatc gctaagtctt
     6721 cggatttcaa cttggcggtg ccctcaacgg ccaaggcatt cgccacggcc gacttaatgg
     6781 ttttctgcag cggaaccgcg gcggccttgg ggacatagtc cagaagggcg atggcttcat
     6841 ccaccgactt accgcgaact aacgccgcca cccgccgcac tttgcgcggg gacatccgct
     6901 ggaatctggc tcgggcgatt gcttccattg gactcacttc ttgggcgctt tctcacccgg
     6961 cgcgggtgcc ggcggcgggg gtgcagcacc gccctcggcc tccttggcgg ccagttttcc
     7021 ggaatggccg cggaaggtcc gggtgggtga aaactcgcct aatttgtgcc ccaccatgtt
     7081 ctcggtgatg tagaccggaa tgaacttgtt gccgttgtgc accgccagcg tatgacccac
     7141 gagttcgggc gcaatggtgg aacgccgcgc ccaggttttg agcaccgttt tgaccccggt
     7201 ctcgttcaac ttcaaaatct tcttcatcaa cttggcgtcc acatagggac ccttcttgag
     7261 tgagcgtgcc atagtattag tcgcgccttt tgagaatgta acggtccgag ggcttgtgct
     7321 tgcgcgtctt ctggcctttg gattttaagc cccagggcga gcaggggtgc cggccgccgg
     7381 acgatttccc ttcgccgccg cccatcggat gatccaccgg gtttttggcc actccgcgca
     7441 cccgggggcg gtaacccagc cagcgggtgc ggcccgcctt gcctaaggaa atcttctcgt
     7501 gatcgagatt gccgacctgg ccgatagtgg cgtaattctc cagccggacc agccggattt
     7561 cacccgaagg caagcgcagg tgaccgtaat ttccctcctt ggccacgagc tggaccgatg
     7621 caccggcgct gcgggcaatc tggcccccgg cgccgcgccg gagttccacg ttgtgtacca
     7681 tcgtccccag agggacgcgg gctaaaggca cggcgttccc cggccggatc tccgcttcgg
     7741 gcccggccac gagggtatca ccgacggcta aatccagcgg ggctatgatg taacgctttt
     7801 cgccgtcggc gtagtgtaaa agcgcaatcc gtgctgagcg gttcggatcg tactcaatgg
     7861 cggccacttt cgccggaacg tttgctttgt cgcgcttgaa gtcgagcacc cggtagaact
     7921 gcctgtgccc gccgccgatg tagcgggcgg tgatatgccc gtcgttgttt cgcccgcccg
     7981 atttgcgcaa aggctccaga agggaacgct ccggctggct gcgggtaatc tcggcaaaat
     8041 cggagaccgt gatccagcgc cgcgaggggg tgaacggttt ataggtcttg atgcccatca
     8101 gatcttctcc agggcctcaa tcgtctgtcc ggttttcagc tgcactatcg ctttcttcca
     8161 gttcggccgc gtgccggtgc gctgccggaa gccgtagcgc cgggttttgc cgcgcacaat
     8221 ctgggtgttg accccggtga cgtttacttt gaaaagggtt tccaccgcgc gcttgacctc
     8281 cagtttgttc gctcccgtgg ccacgtggaa aaagtactta tgctcttttt cccggaggcg
     8341 cgtccccttc tcggtcaaca ggtgccgcag gatcaccgtg cgcgcgtcct tcatccggaa
     8401 aaaacctcct caacggccgc cagggccggt ttggtcaaaa ggacggagtc ggcgatccac
     8461 aggtcgtagg cgttgacggc ggcggcccgc ttgaacgtga caccgggaat gttccggcac
     8521 gaaagt
//
LOCUS       DBNGZA010000341         3557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1977434, whole genome shotgun sequence.
ACCESSION   DBNGZA010000341 DBNGZA010000000
VERSION     DBNGZA010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1977434"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="purM"
                     /locus_tag="AC16FU_07635"
     CDS             complement(<1..884)
                     /gene="purM"
                     /locus_tag="AC16FU_07635"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR2994276.1"
                     /translation="MPSKLTYREAGVDIDAASASVEKVKQLARATFNENVLSEIGSFG
                     GMFAFPGGYKNPVLVSSADGVGTKLKIAFAANRHDTVGIDLVNHCTNDILVHGARPLF
                     FLDYLATGKLDSTIVQQVITGLATACRTVGMALLGGETAQMPDFYQPGEYDLAGFIVG
                     VVERKKIIDGKKIEPGDLLLGIPSNGLHTNGYTLARKALFEIAGLNVNTILPELGEKL
                     GDVLLRSHRCYWPVVKDLLPSGHLKGLAHITGGGIAGNLVRILPEGCRAVIDRKTWPV
                     PPIFEVIARRGNIGNEEM"
     gene            complement(1040..1699)
                     /locus_tag="AC16FU_07640"
     CDS             complement(1040..1699)
                     /locus_tag="AC16FU_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR2994277.1"
                     /translation="MLTVDDVKKNPTVRTLVEKADKVLETMGYTEHGLRHCSLAANIA
                     HNIMARLKKSEAEAQKAAIAAYLHDIGNLINRDYHAQTGAVMAFHVLSEMGMPLEDRL
                     DITSAIGNHDEKDGYPVSDIGAAVILADKSDVHRSRVRNQDMIKHDIHDRVNYAATSS
                     FLRIDEEKRTASLEITIDTSISQVMEYFEIFLSRMVVCRRAAEFLKLRFELEINGNRL
                     L"
     gene            complement(1888..3549)
                     /gene="priA"
                     /locus_tag="AC16FU_07645"
     CDS             complement(1888..3549)
                     /gene="priA"
                     /locus_tag="AC16FU_07645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR2994278.1"
                     /translation="MTEITATTPLPLPLTPEQRQACGAITQAMELRKFQPFLLWGVTG
                     SGKTQVYLEAIRETLKRDKSAIVLVPEISLSSQLAQRFKAAFGGQLAFLHSGLSAGER
                     ADSWRKIKTGEKKVVVGVRSAIFAPVADLGLVVVDEEHADTYKQADPAPRYHARDVAV
                     MRAKENDAVAILGSATPSLESFYNAQKGKYHLLELPVRVLARPLPNVEIIDLKQNPPG
                     GTFSGPLVLAVKKCLTENQQVLLFLNRRGFAGLLKCGDCGFSFHCPNCSTSLTYHRIN
                     QTLRCHFCDRCEKPPDTCPCCRSKKFLYLAHGTQRVEAELKNLFPGTRFERMDTDTTR
                     GKLASFHLLDRFARREFDLLLGTQMIAKGLDFPDVGLVGVVSADLSLHLPDFRAKEKT
                     FQLLTQVAGRTGRGDRPGQVLIQTYAPDEPVIQQAARHDYRSFYQKEIGERLALSYPP
                     FSRLIALIFRSKQEKSAEQVAGLFALKIKREVTRQKMAQIQILGPAPAPFYKLRKEYR
                     WRVTLKAKRVVDILPSIAKILESEPAFRPRPLLRITVDVDPQDLL"
BASE COUNT          723 a          917 c         1125 g          792 t
ORIGIN      
        1 cacatctcct cgttgccaat attcccgcgg cgggcgatga cttcaaaaat cggcggcacg
       61 ggccaagtct tgcggtctat gactgctcga cacccctccg gcagaatgcg aacgagattc
      121 ccggcaatac cgccgccggt gatgtgggcc agacctttca aatgtccgct gggaagcaaa
      181 tctttcacca ccggccagta acaccggtgg gagcggagga gaacgtcgcc taacttttcg
      241 cccaattcgg gcaggattgt gttcacgttc aaacccgcga tttcaaagag tgccttgcgg
      301 gccagcgtgt agccattggt gtggaggccg ttggagggga tgccgagcaa gaggtcgccc
      361 ggctcgattt ttttgccgtc gataattttc ttccgctcca ccactccgac gatgaaaccg
      421 gccaggtcgt actcgcccgg ctggtagaag tcgggcattt gcgcggtttc gccgcctaaa
      481 agggccatcc ccaccgtgcg gcaggcagtg gccaagccgg tgatgacctg ctgaacgatc
      541 gttgaatcga gttttccggt agcaaggtag tcgaggaaaa agaggggccg ggcgccgtgc
      601 acaaggatgt cgttggtgca gtggttgact aagtcaatgc ccaccgtgtc gtggcggttg
      661 gcggcgaaag cgattttgag tttggtgccg acgccgtcgg cgctggagac taaaacagga
      721 tttttgtagc cgccagggaa ggcgaacatt ccgccgaagg agccgatttc ggagaggacg
      781 ttttcgttaa acgtggcgcg ggctaattgt ttaacttttt cgacagaggc ggaggcggcg
      841 tcgatgtcaa caccggcctc gcggtaggtg agtttggagg gcatacgcga aagaatataa
      901 tcaaaaacgt ggtttatagc gcaggttaag taaaccaacc tgcgctacaa aagcaaaaac
      961 aggcggcact aagagtgccg cctacgcggg gggatgatac gctactgaag tcgcgcctcc
     1021 ccggtgcagg aggagggctt tacagcaatc ggttgccgtt gatttccaat tcgaagcgga
     1081 gtttcagaaa ctcggcggcg cggcggcaga ccaccatccg ggagaggaaa atctcgaaat
     1141 actccatcac ctgcgagatg gaggtgtcga tcgtaatctc caacgatgcc gtgcgcttct
     1201 cctcgtcaat ccgcaggaag gaactcgtcg ccgcgtagtt cacccggtcg tggatgtcgt
     1261 gcttgatcat gtcctggttg cgcacccgcg agcggtggac gtctgacttg tcggccaaaa
     1321 tcaccgcggc accgatgtcc gaaaccgggt agccgtcctt ctcgtcgtgg ttgccgatgg
     1381 cggaggtgat gtccaaacgg tcttccagag gcatccccat ttctgagaga acgtggaaag
     1441 ccatcaccgc gccggtctgg gcatggtagt cacggttgat gagatttccg atgtcgtgca
     1501 ggtaggcggc gatggcggcc ttctgggctt cggcttcact tttcttaagc cgcgccataa
     1561 tgttgtgggc gatattggcg gcgagcgaac agtgcctgag accgtgctcg gtatagccca
     1621 tcgtttccag aactttgtcg gctttttcca ccagcgtgcg gacggtgggg ttctttttta
     1681 cgtcatcgac ggtaagcatc gggccgtttt tgtggttgcg gtttttattg cgcccggaaa
     1741 tggtcttggg ctcggggtta gggacagtta gagtattcgc tgatacattc ggattcatag
     1801 ccgctcctgg cttttcggga ggtgattgct ccacatcttt aatacggcat tttctgggag
     1861 ttctggagcg tggattccgg gagaaaatca gagcaaatcc tgcgggtcaa cgtccacggt
     1921 gatgcgcagg agggggcggg gacggaaggc cggttcgctc tccagaattt tcgctatgga
     1981 tggcaggata tcaaccaccc gtttggcctt gagggtcacc cgccagcggt attctttccg
     2041 caacttatag aagggagcgg gtgcgggacc gaggatctga atctgcgcca ttttttgacg
     2101 ggtgacttct cttttgattt tcagtgcaaa taggcctgcc acttgctcgg cggatttctc
     2161 ctgttttgag cggaatatca gggcaatcaa acgagagaaa ggcgggtagg agagcgccag
     2221 tcgttcgccg atttccttct ggtaaaatga ccggtaatcg tgccgcgcgg cctgctggat
     2281 gaccggctcg tccggtgcgt aggtctggat caatacctga cccggacgat caccacggcc
     2341 ggtacgcccc gccacttgag ttagcagctg aaacgttttc tctttggctc ggaagtcggg
     2401 gagatgaaga ctgagatcgg cggagacgac gcccaccagg ccgacgtcag ggaaatcgag
     2461 gcccttggcg atcatctggg tgcccagaag gaggtcgaat tcgcggcggg caaagcggtc
     2521 gagcaggtgg aaggaagcga gcttgccgcg cgtggtatcg gtatccatcc gctcaaaccg
     2581 ggttccgggg aagagatttt tcagttccgc ttcaaccctt tgtgtaccgt gcgccagata
     2641 aaggaatttt ttgctccggc agcagggaca ggtatcgggc ggcttttcgc agcggtcgca
     2701 gaagtggcag cgcaaagtct gattgatccg gtgataggtg agagaagttg agcagttggg
     2761 gcagtgaaag gaaaatccgc agtcgccgca cttgaggagg ccggcaaaac cgcgccggtt
     2821 caagaacaaa agcacttgtt ggttttctgt caaacatttt ttgacggcga gcaccagcgg
     2881 gccggaaaaa gtcccgccgg gcgggttctg tttcaagtcg atgatttcta cgttgggcag
     2941 cggtcgggca agtactcgaa caggcagttc cagaaggtga tactttccct tctgcgcgtt
     3001 gtagaatgat tccaagctgg gcgtggctga accgaggatc gcgacggcgt cattttcttt
     3061 cgcccgcata acggccacgt cgcgggcgtg gtagcgcggg gcgggatcgg cctgcttgta
     3121 ggtgtcggcg tgctcttcgt ccaccaccac caaccccagg tcagccaccg gagcaaagat
     3181 ggccgagcgg acgccgacca ccactttttt ctcaccggtt ttgattttgc gccaggaatc
     3241 ggcccgttcg ccggcggaca agccggagtg caagaaggca agttggcctc caaaggcggc
     3301 cttgaagcgc tgggccaact gcgaggagag ggaaatctcc ggcaccagaa cgatggcgct
     3361 tttgtcccgc ttaagcgttt cccgaatggc ttcgaggtag acctgggttt tgccggagcc
     3421 ggttacgccc cagaggagaa agggctggaa tttcctcagt tccatcgctt gtgttatcgc
     3481 accgcaggcc tgtctctgtt ccggcgttaa tggtaatgga agaggggttg tggccgtgat
     3541 ttccgtcagt gggtcgc
//
LOCUS       DBNGZA010000342         1274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1657944, whole genome shotgun sequence.
ACCESSION   DBNGZA010000342 DBNGZA010000000
VERSION     DBNGZA010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1657944"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1274)
                     /locus_tag="AC16FU_07650"
     CDS             complement(<1..>1274)
                     /locus_tag="AC16FU_07650"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR2994279.1"
                     /translation="QGIAAGGPNASTQAAAKENLRVATDGRQPGQSASIRIATVTLQG
                     GNINFSDHFIKPNYSANLVQIGGRVSGLSSEPDKAADVDLRGRLDNSAPLEITGKLNP
                     FSKDLYVDLGVNFKDIDLSPMTPYAGKYAGYAIEKGKLTLTLKYLIENRKLSAQNKVF
                     IDQFTFGEETNSPDATKLPVRLAVSLLKDRNGAITLDFPVAGSLDDPQFSVWGVIVQV
                     ITNLLTKAATAPLALLGAVFGEGEEAANSLEFDYGRANLDGAAEEKLKSLATALVDRP
                     SLKLEISGYADTEKDVEGLRQYRFERKLKAEKLAETTKKSEADVSLDELKIEPDEYSK
                     YLALAYKKETFPKPRNIIGLAKDLPDAEMEKLMLTHLEVSEADLEALAERRAKTVEDY
                     LLQTGQVDSERVFLVKAKTLGPEKKEGLKDSR"
BASE COUNT          239 a          373 c          342 g          320 t
ORIGIN      
        1 acggctgtct ttcaagccct ctttcttctc tgggcccagg gttttcgctt taaccagaaa
       61 aacccgctca gagtcaacct gccctgtctg taagagatag tcttctacgg tcttcgcccg
      121 ccgctcggcc agtgcttcga ggtcagcctc ggagacctcg aggtgcgtga gcatgagttt
      181 ttccatttcc gcgtccggca ggtccttggc caatccgata atattgcgcg gtttcgggaa
      241 cgtttccttt ttatacgcca acgccaggta cttggaatac tcgtctggct caattttcag
      301 ctcgtcgagg gaaacgtcgg cttcactttt tttcgtggtc tcggccagtt tctcggcttt
      361 cagcttgcgc tcgaagcggt attgccgcag tccttctaca tccttttccg tatccgcgta
      421 tccagaaatt tccagtttga gactgggtcg gtcgacgagc gcggtggcca ggcttttcaa
      481 cttttcttcc gccgcaccgt ctaaattggc tcggccgtag tcgaactcca gagaatttgc
      541 cgcctcctcc ccttcgccga aaaccgcgcc gagcagcgcc agcggtgcgg tcgcagcctt
      601 ggtgagaagg ttggtgataa cctggacgat taccccccag acactaaatt ggggatcatc
      661 gagagagccg gccacgggaa agtccagggt aatcgcccca ttcctatcct tcaacaacga
      721 gaccgccagc cgcaccggca acttggtggc gtccgggctg ttcgtctctt caccaaaagt
      781 gaactgatcg ataaagacct tattttgcgc gctgagctta cggttctcga tcagatattt
      841 gagggtcagc gttaacttcc ctttctcgat ggcgtaccct gcgtacttcc cggcatacgg
      901 cgtcatgggg ctgagatcaa tgtctttaaa attgaccccc aaatccacat agagatcttt
      961 gctaaatgga ttgagtttgc cggtgatttc cagcggggca gaattgtcga gccggccgcg
     1021 caggtcaacg tctgccgctt tgtcgggctc agacgacaaa ccagacaccc tcccgccgat
     1081 ctggacgaga ttcgccgagt aattcggctt gatgaagtgg tcagagaagt tgatgtttcc
     1141 gccttgtaag gtgaccgtag cgatgcggat agaggcggac tgcccgggct gccgcccatc
     1201 tgtcgcgacc ctcaggttct ccttagccgc cgcctgggtg gaggcgttcg gtccgccggc
     1261 ggcgatccct tgca
//
LOCUS       DBNGZA010000343         4730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2824672, whole genome shotgun sequence.
ACCESSION   DBNGZA010000343 DBNGZA010000000
VERSION     DBNGZA010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2824672"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="AC16FU_07655"
     CDS             complement(<1..1661)
                     /locus_tag="AC16FU_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HIR2994280.1"
                     /translation="MKRLSLTVALLVAGLAPAQARIIVAPPPGQLNFPPYLSIKNYDV
                     RVKITGPMAVTTIEQVYQNDCAQMLEGTYLFPIPEGAALSKFYLYVNGEEVPGQVLAA
                     GEARQIYEEIVRRLRDPALLEFAGCGLFKARIFPIPAHGDAKIKLSYSQVLKPFNGLF
                     EYKYSFGREALVQTGINNLTFDLQLASSVPLKNVYSPSHEVKIDRSDEKSARVNWSEN
                     SREIGGDFVLYFAVSPKDIAFSILTDRDAGENYFMALLSPEVKPSATKTIFKDIVFIL
                     DISGSMRGEKIDQAKAAVLFCLQSLNAADRFNLVAFNDQVRVFKEGMAAATPVNIREA
                     EVWVAQIEAEGGTNIAGALEAGLKQLAVGRLNYAVFLTDGLPTVGTTDVNEILALAKR
                     ANAGRVKIFDFGVGYDVNTHLLDKLAEQNSGLSDYVIPSENIEVKVSSFYKKISAPLL
                     ANLKLNFGAVKISDVYPNELPDLFAGSQLVVVGKYSGPEQTTVTLTGELNGEKVVYEY
                     PADFSAKTSWEKFIPKIWATRRVGYLIDQIRLSGESREVVDEIVTL"
     gene            1929..2198
                     /locus_tag="AC16FU_07660"
                     /pseudo
     CDS             1929..2198
                     /locus_tag="AC16FU_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
     gene            2681..3190
                     /locus_tag="AC16FU_07665"
     CDS             2681..3190
                     /locus_tag="AC16FU_07665"
                     /inference="COORDINATES: protein motif:HMM:NF046894.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HIR2994281.1"
                     /translation="MARQRTGGAALEESSLRPKFESKNRKKGSAETFLFATGRALDFA
                     LEGPGFFVVEAPAGRRLTRWGHFYLDGQRRLCTGEGHAVAGEQGLLALPPGALTVTPT
                     GDLRVNRQRTGTLALVLPVAPAPAPKTFVRQGYLEISAGEVAGEMGRSFYRRKNQKDD
                     SPREGENCG"
     gene            3436..3894
                     /gene="flgN"
                     /locus_tag="AC16FU_07670"
     CDS             3436..3894
                     /gene="flgN"
                     /locus_tag="AC16FU_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016985.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HIR2994282.1"
                     /translation="MKKTEELVSRLIEIIQQETYNFGEFLALLQEERRLLEEGRLAEM
                     ERIVSTQEHVVACGQMLEEERRAVTAQLAETFSLPTDQFSLGALLPRVGSFQSGRLAE
                     LQGQLVDLYQKIQRAKHQIALLLRQSPLGQTTTKLTPPSMPIPPRHFAAQ"
     gene            4006..>4730
                     /locus_tag="AC16FU_07675"
     CDS             4006..>4730
                     /locus_tag="AC16FU_07675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2994283.1"
                     /translation="MRIFTAPNFLLYLPVILLSVPLSGMAQKQVDLLLQNGRVATLDT
                     QQPWAEAVAMAGDSILAVGATVELEKFSGPRTKVIDLGGRLVIPGFNDAHCHLLSGGL
                     SLLRVNLLGATTAEEIKRRVAEKVRESPPGSWIRGRGWDHTLFNRGRWPDKSLLDSVA
                     DGHPIFLMRVDGHVGWANSEAFRLAGITSATPDPPGGEIVRDPETKEPTGIFKENAVD
                     LINTVVPPPTKTELTLALEKALV"
BASE COUNT          992 a         1295 c         1320 g         1123 t
ORIGIN      
        1 agtgtcacta tttcatccac cacttcccgc gattctccgg aaagacgaat ctgatcaatc
       61 aaatatccca cccggcgggt ggcccagatt ttgggaatga atttttccca gctggttttg
      121 gccgaaaagt cggcagggta ctcgtagact actttttcgc cgtttaattc tccggtcaaa
      181 gtgacggtcg tttgctccgg cccggagtat ttcccgacca ccaccagttg cgaaccggca
      241 aaaaggtcgg gcaattcgtt cgggtagacg tctgagattt tgaccgcacc gaaattcaat
      301 ttcaagttcg ctagaagggg agcggaaatt ttcttgtaga agctggagac tttgacttcg
      361 atattttcgg aggggatgac gtaatcggaa aggccggagt tctgctccgc cagtttgtcc
      421 agcagatggg tgttcacgtc atacccgacg ccgaagtcaa aaattttaac ccgcccggca
      481 ttggcccgtt tggccagcgc caaaatttca ttcacgtcgg ttgtgccgac cgtgggcaga
      541 ccatcggtca gaaacaccgc gtaatttaac cgacctacgg ccaactgctt taaccccgcc
      601 tccaaagcgc cggcgatatt ggtgccgccc tcggcttcaa tctgggctac ccagacctcg
      661 gcttctctga tattcaccgg ggtggctgcg gccatccctt ccttaaaaac ccgaacctga
      721 tcgttgaaag cgacgaggtt gaaccgatcg gcggcgttga gactctgcag gcagaagagc
      781 acggcggctt tggcctgatc gattttctcg ccccgcatac tgccggagat gtccagaatg
      841 aagacgatgt ctttgaaaat cgtcttggtc gcggagggtt ttacctccgg cgaaagcagg
      901 gccatgaagt agttttcacc ggcgtcccgg tcggtgagga tggaaaaggc aatgtctttc
      961 gggcttaccg caaaatagag aacgaagtca ccgccaattt cccgggagtt ctcgctccag
     1021 ttcacccggg ccgatttttc atccgaccgg tcaattttga cttcgtggga gggcgagtag
     1081 acgtttttca acggcacgct ggaggccaac tgcagatcga aggtcaggtt gttgatcccg
     1141 gtctgcacca gcgcttcccg gccaaaactg tacttatact caaataagcc gttgaacggt
     1201 ttcagcacct ggctgtagga gagtttgatt ttggcatcgc cgtgcgccgg gatgggaaag
     1261 atgcgcgcct tgaagagtcc gcagccggcg aattccagca aggccggatc tcttaacctc
     1321 cgcacgattt cttcgtaaat ctggcgtgct tcgccggcgg ccagaacttg gcccggcact
     1381 tcttcgccgt tgacgtacag gtaaaatttg gacagggcgg ccccctccgg aatcggaaaa
     1441 agataggttc cttccagcat ttgcgcacag tcattctggt acacctgctc gatggtggtg
     1501 acggccattg gcccggtgat tttcaccctt acgtcgtagt ttttgatgga caggtagggc
     1561 gggaagttca gctgtcccgg cggcggggcg acaatgatgc gggcttgggc gggagccaat
     1621 ccagcgacca aaagcgcgac ggttaacgac aggcgtttca tgattcctcc tttgctctcc
     1681 ttttcatacc cacgggagaa gattttattc cctgactttt ttgctttttt cattcgcaag
     1741 gggcgccggt cgagccaaga ggatagcccg gcggtcacgg gggggaatga gtgttttttt
     1801 gcctttttcc ctctgaaagc agttgacatc cccagagggg ggtgctattt ttcccactga
     1861 tgctgccggc tcagaacggc cggcgggaag aaagagcggt cgaaccgtaa cctgtttcac
     1921 aggaggaaaa ggtgatggca acgaagcgga aaccgagtgc agcgttcatg aagcccctga
     1981 agcccagcgc ggccttaagc gccgtcgtgg gtgaaaaagc cattccccgc accgaagtca
     2041 ccaagaaggt gtgggcctac atcaagcgga agggcctcca ggacaagaag aaccggcgga
     2101 tgattaacgc cgacgagatg ctcaaggcgg tgtttggcgg caagcggcag gtcaacatgt
     2161 tcgaaatgac caagctgatt tccaagcacc tgaaataggc ggggttttag ttttaccgtc
     2221 atttgcatag agcctttgct cgttaagagc gaaggggaat aatccttttt ccttcagaac
     2281 tctccccctc aaagggaagc gggcaggggg tgtttttacc tttttgggtc gatttgtttt
     2341 ggcagcgcgc caaactgctc aaactttttg gcagtggctc tctatagttt tttccttttt
     2401 tgttctgcat cactactcag acttttctaa aatttctccc ttaggaaaat tctaccaata
     2461 acgggtggaa cctttttccg tttccggttt tgttgtgtgt cggttcttct cccattgcgt
     2521 cttctcttcg attcccgggg cacaaatagt tccttctaat ataatatcag tcatatagtt
     2581 attcccgaac ccaatcttct cctctcagga gccacaaata agtctggctc aatggccgct
     2641 ggaaatcgcg tggcacacaa gttgcaaaac cttgggttgt atggctcgcc agcggaccgg
     2701 tggtgctgcc ctcgaagagt cgagtcttcg accgaagttt gagtcaaaga accgcaaaaa
     2761 aggttcggct gaaacttttc ttttcgctac gggccgcgct ttggactttg ccctggaagg
     2821 cccgggattt ttcgtcgtcg aagcaccagc cggccggcgg ctgacccgct ggggccattt
     2881 ttacctggac ggccaaagac ggctttgcac cggagaaggt catgccgtag ccggtgagca
     2941 gggccttctc gctttgcctc ccggagctct gacggtcaca cccaccggtg acctccgggt
     3001 caatcgccag cggacgggaa cactggcgct cgttctcccc gtggcaccgg cacccgcccc
     3061 gaagacgttc gtgcgtcagg gatacctgga aatctccgcc ggcgaggttg ccggagaaat
     3121 ggggcggtca ttctaccgcc ggaagaatca aaaagatgac tcgccgcggg agggagaaaa
     3181 ttgcggctga ttcttttagt cattttcctc ctgctgcttt tggccttacc ttactcggcg
     3241 cgccccgcaa ctattcccgc ttcccggccg gtgccgcacg gtgctactcc gtagctatcg
     3301 tttttactct ttttgcattg tcgggtacac caagataaag ttttcctccg tgtcctctgt
     3361 gtctctgtgg tagaatccgg ccttaccatc tttttccatt caagtccgca ccgtcgatgc
     3421 cgaatgttcc tgtagatgaa aaaaaccgaa gaactcgtca gccggctgat cgaaatcatc
     3481 cagcaggaga cgtacaactt cggggagttc ctggcgcttt tgcaggaaga acggcgcctc
     3541 ctcgaggagg ggcggctggc tgagatggaa cgtattgtga gcacccagga gcacgtcgtt
     3601 gcgtgcgggc agatgttgga agaggaacgg cgcgccgtca cggcccagtt ggccgaaaca
     3661 ttcagtctac cgacggatca gttttcgctg ggagcgctgt tgccgcgggt gggaagtttc
     3721 cagtcgggcc ggctggccga actgcaagga cagttagtgg atctctacca gaagattcag
     3781 cgggccaagc atcaaattgc ccttttgctc cggcaatcgc ctctgggcca aaccacgaca
     3841 aaactgaccc caccctcgat gcccataccc cctcgtcatt ttgccgcgca atagcgttgg
     3901 cttgaattgt acccgattca gaacgtacgt cttctcccgc aacttgtctt aggcagaaaa
     3961 gacactattt ttacttccgt gtgcccaaaa gctatcgtcc gacgattgcg gatattcacc
     4021 gctcctaact ttctcctcta tctccctgtg attctccttt ccgttccact ttccggaatg
     4081 gcgcaaaaac aggtcgacct gcttttgcag aacggccggg tggccacgct agatacgcag
     4141 cagccctggg ccgaggcggt ggctatggcc ggggattcga ttctcgcagt tggtgctacc
     4201 gtcgaactcg aaaagttttc ggggccgcgg acaaaggtaa tcgatctggg cggtcggctt
     4261 gttattcccg gcttcaacga cgcccactgc catctcttga gcggcgggct gtcgcttttg
     4321 cgggtcaatc tcctcggcgc cactacggct gaagagatca agcgtcgcgt cgccgagaaa
     4381 gtgcgggaat caccgccggg gagttggatt cgcgggcggg ggtgggacca caccctcttc
     4441 aaccggggcc ggtggccgga taagtcgctc cttgattcgg tcgccgacgg tcatcccatt
     4501 ttcctcatgc gggtggatgg ccacgtgggc tgggcaaatt ccgaagcgtt caggttagcc
     4561 ggaatcacga gcgcaacgcc ggatccgccg ggcggggaaa ttgtacgcga tccggaaacc
     4621 aaagagccga ccggcatttt caaggaaaat gcagttgatt tgataaatac ggtcgttccg
     4681 ccgccgacga agaccgaatt gacgctggcg ttggagaaag cgctcgtgga
//
LOCUS       DBNGZA010000344         6216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2928594, whole genome shotgun sequence.
ACCESSION   DBNGZA010000344 DBNGZA010000000
VERSION     DBNGZA010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2928594"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16FU_07680"
     CDS             <1..439
                     /locus_tag="AC16FU_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR2994284.1"
                     /translation="NAGVYVLDSNRLNASQTLKFTHPPTQSSVPKYILGRDTVGLTAV
                     REEHNSSLPTSFALSQNRPNPFNPTTTITFALPKESEVTLEVFNLLGQKVIDLVDEHL
                     SAGTKSVEWDGRDALGREVPSGIYFYRMRAESFAETKKMVLMK"
     gene            656..2833
                     /locus_tag="AC16FU_07685"
     CDS             656..2833
                     /locus_tag="AC16FU_07685"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR2994285.1"
                     /translation="MVTEITRGAMLRIVEGKFQIGKELFSPHSVEVHYFRLSKRYWSV
                     CFERIKRIGFRILSTAVPWNLHEDVRGGFDFSGTVDSRKDLIVFLELAREFGFKVILR
                     PGPWIGAEWPNGGYPDYLINMPELLARDAQGNPVPNRLRAGVKAGFVPSYHHPRYQSA
                     VKRYFQALGDTIKHYVYPRGPIFLFELDHQASFGGNLDPFAADYNQFTLSTLYPQFLE
                     RKYQETKRLNQTYGERHAAFTAVEPPRSVPGKRGLGYPKFFDWIAFRQFLLQDYVAGL
                     KESLKQVDLSALFYFTQPALGSRLCLPPLAGSESGEVFSGVSLPDGDDFSGAAALLRQ
                     AAGQRQFAWVSDLPLGRAAADPEQTKKHLPVHAADIKRTVVQAAASGVQGINFNSFVE
                     RDHWYGAPLAADGTVQPAYEILQKFTLAHEKFPWQPTSLSHSVGLAAYQPYLWLEKTL
                     GPANPGQNWFTSRLQEGISRDLEYLKVGFGEVETTAASELERYSLFILAVAEFMALET
                     QEQLLRLIQSGKIGLFVGTLPRVDLAGKSCTLLAKGLRTSTAPFSGVERVKGFGQEFS
                     AALFGTIRFSRLKKICTAGGRTVGAGGKVGKGSAFVLTFDPVSHLQPHKLAFWEALFR
                     SLGIARPVATSDPQVIVSLHQNEGRTLAAVLLPPAAAPAGTRRQIIFQMDTRALGIRG
                     DRLRLADLLGQVTLRTSASELRRGIVLEIAPGDSFLFLIDKRS"
     gene            complement(2903..3973)
                     /locus_tag="AC16FU_07690"
     CDS             complement(2903..3973)
                     /locus_tag="AC16FU_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2994286.1"
                     /translation="MSALWALPLSFSYPATTATVQAETLPPPIPPKPPRPPKKKKEIQ
                     RRGYVLGESLFIEPQPPAAVPGYRNQKGTAFALAGEITVAEDEEATGDVVVLFGSIEV
                     FGRVHGNVVSVMGNVHLGPRAVVDGDVVAANLAVEPGAQIHGDRVEIPFPNMGFPFGR
                     DFFRHGGGLLLAVILLTISSLFGILTLAVAPRQVAKVNQKIDSGLFKSFFVGFVGEIL
                     LLPIFILLCVTVIGIPVAFLIFPLLVVAAYLLGNTAISLSVGQKLRQGTQSPGASSMI
                     TMLSGKFFLYLPFLCAGFLGLFGGGFGPLFRTFSLIFFLFGLALHFAATASGFGAALL
                     SRYGTRPKEPPPPPPAAHMQPT"
     gene            complement(4075..5175)
                     /locus_tag="AC16FU_07695"
     CDS             complement(4075..5175)
                     /locus_tag="AC16FU_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR2994287.1"
                     /translation="MNFRDKLTQRNVFRVATGAIAATALLLFSFAESQSRQYTYEFHK
                     ELPVSPGFVFSLTDPIGDIVVEGYAGRSIEIAARKVVEASSRRKAAEWEREVRIEIDR
                     RENQVTLKTEYPVHRTRNNLFDFLYSDDSHPRGQVNYRIRIPESGTVSLHTTSGNCYV
                     AHAKDRVELNSTSGDLEIIGIDGAVLIETTSGDVIVREVSGNLDLATTSADLTIADAR
                     GALRIKSVSGDIHLSRLTGDLTVESTSGDVQADGVQGEVRVTTVSGDVTVSGQTGGCY
                     LTTISGDIETKIAQLTGASALATTSGDIHLFLPEEADAALSIATRGGEIETHLPLALQ
                     SVKRHQVEGTLGRGSHRLEMKSTSGDIAVASF"
     gene            complement(5200..5784)
                     /locus_tag="AC16FU_07700"
     CDS             complement(5200..5784)
                     /locus_tag="AC16FU_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR2994288.1"
                     /translation="MQARETAESDQALVVQALAGEKKAFAALLERHRQAVFHITYQIA
                     HNQEEARDLVQETFMKAFAGLATYRSQFRFTTWLYRIAANCAIDALRKKKIDALSLDQ
                     PLETGEGTVAFEVADLAADPEAELVAEQRRISIGEAIDALPKKYREVLVFRHREEKSY
                     EEISDILKIPVGTVKARIFRARELLKKKLKHYRP"
     gene            complement(5941..>6216)
                     /locus_tag="AC16FU_07705"
     CDS             complement(5941..>6216)
                     /locus_tag="AC16FU_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994289.1"
                     /translation="RSYEADAENVSTEDLIQQILAKVPLSLASHPQNMVIVQRPVMAA
                     ISNLCKMTLLAAGQRLSSDVINLPTDRVTVAKTIALLFRARKAKARS"
BASE COUNT         1282 a         1786 c         1823 g         1325 t
ORIGIN      
        1 caatgctgga gtttacgtct tagattcgaa ccgactgaat gccagccaaa cactaaaatt
       61 cactcaccca ccaactcaga gtagtgttcc aaaatacatt ctgggtcggg acacggtcgg
      121 tctcacggca gtgcgcgaag aacacaactc gagtctgccg acctcgtttg ctctttcgca
      181 gaatcgcccc aatccgttta accccaccac caccattacc tttgcgctcc ccaaagagtc
      241 ggaggtaacg ctggaggtgt tcaatctcct gggacagaaa gtgattgatt tggttgatga
      301 gcacctgtcc gctggaacca agtcggtgga atgggatggg cgcgacgctc tgggccggga
      361 agtgcccagc gggatttact tctaccggat gcgggcggag agttttgccg aaaccaagaa
      421 gatggtgctg atgaagtagt ccggaaatga atttaaaaag ccccgcccag cggggctttt
      481 ttattgattt ccaacaagtt agcagttgta cgactcaccc gctcgtgaaa aaattcaccg
      541 gtaaatggaa tatttattga caacagaaaa attaaccata atttagtccg aaatttccgg
      601 gtaagataaa gctgtgtatg gcggcggtta ttttacacaa gtagttataa tacagatggt
      661 tacagaaatt actcgcgggg caatgctccg gattgtcgaa gggaagtttc agatcggcaa
      721 ggagctcttt tccccccatt ccgttgaagt acattacttt cggctgagca aacggtattg
      781 gtcggtctgt tttgaaagaa tcaaacgaat cggtttcagg atattgtcaa cagccgttcc
      841 ctggaatctc catgaagacg tgcgcggcgg gtttgatttc agcgggaccg ttgattcccg
      901 caaagatttg atcgttttcc tcgaactggc gcgcgaattc ggtttcaaag tgattttgcg
      961 cccaggaccc tggattggag ccgaatggcc caacgggggg taccccgact acttaataaa
     1021 catgccggaa cttttggcca gggacgccca aggcaatccg gtcccaaacc gcttgcgggc
     1081 tggcgtcaaa gccggttttg tcccctccta tcatcacccc cggtaccaat cggcagtgaa
     1141 gcgttatttc caagccttag gcgatacgat caagcactac gtctacccgc gcgggccgat
     1201 cttcctcttc gaactggacc accaggcctc gttcggcgga aaccttgatc cgtttgcggc
     1261 cgactacaac cagttcacgc tttcaactct ctatcctcag tttctggaac gcaagtacca
     1321 ggagacgaaa cgcctcaatc agacgtacgg tgagcgccac gctgctttta ccgccgtgga
     1381 accgccccgt tcggtgccgg gcaaacgcgg tctggggtat ccgaaatttt tcgactggat
     1441 cgcctttcgt cagtttctcc tccaggatta cgtcgcgggg ctgaaggaga gcttgaagca
     1501 ggtcgatctg tcggccctct tctatttcac ccagcctgcg ctcgggagcc ggctttgcct
     1561 gccgcccttg gctggcagcg agtccgggga agtcttttcc ggagtgagtc tgcccgacgg
     1621 agacgatttc tccggtgcgg cggcactcct ccgtcaggca gccggacagc gccagttcgc
     1681 ctgggtcagc gacctgccgc tcggccgcgc agccgccgat ccggagcaga cgaaaaaaca
     1741 tttgccggta cacgccgccg acatcaaacg gacggtggtg caagcggcgg ccagcggcgt
     1801 tcaggggata aatttcaact cgtttgtgga acgagaccac tggtacgggg cgcctttggc
     1861 cgccgacggc accgtccagc ccgcctacga aatcttacaa aagtttaccc tggcccacga
     1921 gaaatttccc tggcagccga cgagtctttc ccactccgtc ggtctggcag cctaccagcc
     1981 gtacctgtgg ttggagaaaa cactgggccc ggctaatcca ggtcagaact ggttcacctc
     2041 ccgccttcag gaaggaatca gccgcgatct ggagtacctg aaagtcgggt tcggcgaggt
     2101 cgagaccacc gcggccagcg aactggaaag gtactctctc tttatcctcg cggtggccga
     2161 gttcatggct ctggagacgc aggagcagct cttacgcctg attcagtcgg ggaaaatcgg
     2221 cctgttcgtc gggaccctgc cgcgggtgga tttggccgga aaatcctgca cgcttctggc
     2281 caaaggtctg cggacgagca ccgcgccttt ttccggggtc gagcgggtca aaggtttcgg
     2341 gcaggagttt tccgcggccc tcttcggcac cattcgcttc tcccgcctga aaaaaatctg
     2401 tacggctggc gggcgcaccg tgggtgccgg cgggaaagtg ggcaagggga gcgccttcgt
     2461 tttgaccttc gatcccgtga gccacctgca gccgcacaaa ctggcgttct gggaagctct
     2521 cttccgctcg ctggggattg cccgtccggt tgccaccagc gacccgcagg tgatcgtttc
     2581 gctccaccag aacgaagggc gcacgctggc cgcggtgctc ctcccgcctg ccgcagcccc
     2641 ggcgggcacc cgccgccaga tcatcttcca gatggacacc cgagcgctgg ggattcgggg
     2701 agaccgcctg cggctggcag acctattggg tcaggtgact ctgagaactt cggcgtctga
     2761 actccggcgg ggaatcgttt tggaaatcgc cccgggcgat tcgttcctct ttttgatcga
     2821 caaacgctcg tagggagcat ctcggaaaat cttccccaga gagaaatgca gaagcgccgc
     2881 tgcggtgcgg gggcgctacg gcttacgtcg gttgcatgtg cgcagccgga ggaggagggg
     2941 gtggttcttt gggccgggtg ccgtagcggg agagcagcgc cgcaccgaaa cctgaggcgg
     3001 tagccgcgaa gtgcagagcc aggccgaata aaaagaagat taaactgaag gtgcggaaga
     3061 ggggaccgaa gccccccccg aagagcccca gaaaaccggc gcaaaggaac ggcaggtaga
     3121 ggaagaattt cccgctcaac atcgtgatca tggacgaagc gcccggggac tgcgtgccct
     3181 ggcgcagttt ctgaccgacc gagagcgaaa tggccgtatt ccccaagagg tacgccgcga
     3241 cgaccaaaag gggaaagatg aggaaggcca cgggaattcc tattaccgtg acgcacagca
     3301 aaatgaaaat cggcaaaagc agaatttccc ccacaaagcc cacgaagaat gatttgaaga
     3361 gcccggagtc aatcttctga tttactttgg cgacctgacg gggggctacc gccaaggtga
     3421 ggatgccgaa cagcgacgaa atcgtcagga gaatgaccgc caggagcagt ccgccgccgt
     3481 gccggaaaaa atcccggccg aaagggaacc ccatgttcgg aaaggggatt tccacccggt
     3541 cgccgtgaat ctgcgccccc ggctccacgg cgaggttggc ggccaccacg tcgccgtcca
     3601 cgacggcccg gggccccaga tggacgttgc ccatgacgct cacgacgttg ccgtgcaccc
     3661 ggccgaagac ttcaatactg ccgaagagaa ccacgacatc tccggtggcc tcttcgtctt
     3721 cggctaccgt gatttctccg gccagggcaa aggcggtccc tttttggttc cggtaccccg
     3781 gcactgcggc cggcggctgc ggttcgatga aaagactttc acctaagacg tacccgcgcc
     3841 tctggatttc ctttttcttt ttcggcgggc ggggaggttt tggagggatg gggggtggca
     3901 aggtctcggc ctgcacggtg gccgtcgtgg ccgggtaact gaaagagaga ggcagggccc
     3961 aaagagcact caaaacgaaa agcagtagcc agcgtgcagt ccgccgattc acgaatcccc
     4021 ccggtcattg tcgagaatca gatttttcca aaattccgac atggcgttcg tcctttagaa
     4081 actggccacg gcaatgtcgc cggaggtact tttcatttcc agccggtggc ttccccggcc
     4141 cagggttccc tcgacttggt ggcgcttcac gctttggaga gcgaggggga ggtgcgtttc
     4201 aatttcaccg ccccgggtgg cgatggaaag tgccgcgtcg gcctcttccg gtaaaaagag
     4261 gtgaatgtcg ccgctggtcg tggccaaggc cgaggcaccg gtcagttgcg cgattttggt
     4321 ttcaatgtcc ccggaaatag ttgtaaggta acagcccccc gtttgaccgc tgaccgtgac
     4381 gtcccccgaa acggtggtta cccgtacttc accctgcacc ccgtccgcct gcacgtcacc
     4441 ggaagtgctc tcaaccgtga gatcgccggt tagtcgtgat aggtgaatat cgccggagac
     4501 gcttttgatc cgcagggcgc cccgcgcgtc cgcaatggtc aggtcggcgc tggttgtggc
     4561 aaggtcgaga ttgccggaaa cctcccgcac gataacgtca cccgaggtgg tttctattaa
     4621 cacagcaccg tcaatgccga ttatttcgag gtcgccggag gtgctgttga gttcaacccg
     4681 atctttggcg tgggcaacgt agcaatttcc ggaggtagta tggaggctga ccgtgccgct
     4741 ttccggaata cggatgcggt agttcacctg cccccgggga tggctgtcgt cgctatagag
     4801 aaagtcaaaa aggttgttgc gggtgcggtg caccgggtac tcggttttga gggtgacctg
     4861 gttttcccgc cggtctattt caatccgcac ttctcgttcc cactctgcgg cttttctccg
     4921 gctggaggct tcgaccactt ttcgggcagc aatctcgatt gacctcccgg cgtatccctc
     4981 tacaacgata tccccgatcg gatcggtcag cgagaaaacg aacccggggg aaaccggaag
     5041 ctctttgtgg aattcgtagg tgtactgacg gctctggctc tccgcaaaag aaaagagcaa
     5101 aagcgccgtc gctgcgatgg cgccggttgc gacccggaaa acgttccgct gcgtcaactt
     5161 gtccctgaaa ttcatcggtg gctctcgctt tcgttccggc tacggacggt agtgtttcaa
     5221 tttttttttc agtaactccc gggcccggaa tatcctggcc ttcaccgttc ccaccggtat
     5281 tttcaaaatg tcgctgatct cctcatacga cttttcctcc cggtggcgga aaaccagtac
     5341 ctcccggtac ttcttgggca aggcgtcgat tgcctcaccg atggagatcc gcctctgttc
     5401 ggcgaccagt tccgcctcgg ggtcggccgc caggtcggcc acctcgaagg ccaccgtacc
     5461 ctcgcccgtt tccaggggct ggtccagcga gagcgcgtcg attttctttt tccgcagagc
     5521 gtcgatggcg cagttggcgg cgatccggta gagccaggtg gtgaagcgga actgggaccg
     5581 gtaggtcgcc agccctgcaa aggccttcat aaaggtttcc tgcaccaggt cgcgcgcctc
     5641 ttcctggttg tgcgcgatct ggtaggtgat gtggaagacc gcctgccggt gccgctccag
     5701 gagcgcggca aacgccttct tttcacctgc cagagcctgc accaccaggg cctgatcgct
     5761 ctcggcggtt tctctggcct gcatctcttt cgtacgaggc gcggccgcct gaggtttcgc
     5821 caaagtcgag ccaagataaa cgatgctgcc aacccataca actttttgcc ggagcggcaa
     5881 ggtctagagc gttgctacgg aaaccgtgat gggaggtagt tcaagagaag gaggcggagc
     5941 tcatgaccgg gccttcgctt ttcgcgccct gaagagcaag gcaatcgttt tggcaacggt
     6001 cactctatcc gttggaaggt tgatcacgtc actgctcaat ctttgaccgg cagcgagtaa
     6061 ggtcattttg cataagtttg aaattgccgc cattaccggc ctttggacaa tcaccatatt
     6121 ctgtgggtga gaggcgagag aaagaggaac ttttgcgagg atctgctgga tcagatcttc
     6181 ggtcgacaca ttttcggcgt cagcctcata gctcct
//
LOCUS       DBNGZA010000345         1011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1658316, whole genome shotgun sequence.
ACCESSION   DBNGZA010000345 DBNGZA010000000
VERSION     DBNGZA010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1658316"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16FU_07710"
     CDS             complement(<1..404)
                     /locus_tag="AC16FU_07710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR2994290.1"
                     /translation="MSAVKAVLFDLGSTLLEYENSPWEKLDRLCIASGYRYLAQAGHT
                     LPAAEQFTTVFFDRFRAMWRETERTLAEIDLARFAEEVLQSLGITWRDGEKAGFFEQY
                     YRPIFEQITPYPESAGVLERLSQEGLPLGLVS"
     gene            complement(391..>1011)
                     /locus_tag="AC16FU_07715"
     CDS             complement(391..>1011)
                     /locus_tag="AC16FU_07715"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR2994291.1"
                     /translation="RWILCNTQQSRDLDPAAREILRQFGFPLVVKPHNQGSTVGLSVV
                     HQAGELAPALEAAGKFSGQVLVEEFIAGREITVGVLGDRALPVVEIIPQHEIYDYECK
                     YTKGRSDYICPADLTKEQTEEVQHLGLRAFECLGCSGYARVDFRRSREGKFYCLEVNT
                     LPGMTALSLVPMAAKGAGLDFPHLLEAIIEQALIHQKKKGMTVVRG"
BASE COUNT          210 a          334 c          269 g          198 t
ORIGIN      
        1 ttcgacacca gccccagcgg cagaccttct tggctcagtc tctccagcac gccggcgctc
       61 tccggatagg gagtaatctg ctcaaaaatc ggccggtagt actgctcgaa gaaacccgcc
      121 ttctccccgt cgcgccaggt gatccccaaa ctctgcaaaa cttcctccgc aaagcgggct
      181 aaatcgattt ccgccagcgt cctttcagtt tcccgccaca tcgcgcggaa gcggtcaaag
      241 aagacggtgg tgaactgctc cgccgcgggc agcgtatgcc cagcctgcgc gagatagcgg
      301 tagccggaag cgatgcagag ccggtccaac ttctcccagg gggaattctc atactccagg
      361 agcgtggaac ccaaatcaaa gagaaccgcc ttaaccgcgg acaactgtca ttcctttttt
      421 cttctggtga atgagcgcct gctcgatgat cgcctccagc aggtgcggaa agtcgagccc
      481 ggcccccttc gccgccatcg gcaccaacga gagtgcggtc atacccggta gggtgttaac
      541 ctctaagcag tagaacttcc cctcccggct cctgcggaaa tcgacccgcg cgtagccgga
      601 gcatcccagg cactcgaagg cccgtaggcc caaatgttgg acttcttcag tttgttcttt
      661 cgttaaatcc gcggggcaga tgtagtcgga gcgccccttg gtgtacttgc actcgtagtc
      721 atagatctcg tgctgaggaa tgatctccac caccggcagc gcccggtcac ccaggacgcc
      781 caccgtgatc tcccgcccgg cgatgaattc ctccaccagc acctggccgg aaaatttacc
      841 cgctgcctct aatgccgggg ccagttctcc tgcctgatgc accaccgaaa gccccaccgt
      901 tgaaccctgg ttatggggtt tgaccacgag gggaaacccg aattgccgga gaatctcccg
      961 ggccgccgga tcaagatcgc ggctctgttg ggtattgcac aaaatccagc g
//
LOCUS       DBNGZA010000346         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1869548, whole genome shotgun sequence.
ACCESSION   DBNGZA010000346 DBNGZA010000000
VERSION     DBNGZA010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1869548"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..>1793)
                     /locus_tag="AC16FU_07720"
     CDS             complement(96..>1793)
                     /locus_tag="AC16FU_07720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994292.1"
                     /translation="IAVVLLLAVSLLQLAPPYLTKVAIDDYIKKGNLHGLNGLALLFI
                     LILAVQFVVRYVQTYLMQWVGQKAMFDLRMGIFSHLQKLTLSFYDRNPVGRLLTRVTS
                     DVAALNEMFTSGVVAVFGDLFTLAGIVAIMLYLNWKLALVAFVAIPLLFYATLVFRRK
                     VRAAYREVRLKVAKIMAFLQEQISGMATVQVFNQEKKVFSQFDGVNASLRDSHLRSIF
                     YHAVFFPAVDLIGALSIALIIWYGGGQVLSGFLTLGALVAFIQYVDRFYQPIRDLAEK
                     YNILQSAMASSERIFKLLDTQPEFSAKPGAKELAPVKGAIEFKNIWFAYRGEEYVLRE
                     VSFSVKPGEKIALVGATGAGKTSIASLLCRFYDYQRGSILIDGTELKTINEASLRRNI
                     GLVLQDVFLFSGDIAGNIRLGSEAMPLERVEWAAREVGADRFIRNLPRRYQEEVTERG
                     STLSVGQKQLLAFARALAYDPKILIMDEATSSVDTETERQIQAALERLLANRTAIIIA
                     HRLATVQMADRIIVLHKGQVREEGTHQELLARRGIYYRLYLLQYKDQTERVGERAAV"
BASE COUNT          399 a          537 c          513 g          344 t
ORIGIN      
        1 cttggcacac atcgggcaat ctttgcccgc ggcgggcagc ggaaggaaag cgagaaccac
       61 caccgccgcg aatccaattc ccctcggaaa agcggtcaca ccgccgcccg ttctcccacg
      121 cgctctgtct ggtctttgta ctgcaagagg tagagccggt agtatatgcc ccgccgggcc
      181 aagagttcct ggtgggtgcc ttcctcccgc acctgtcctt tgtgcaatac gatgatccgg
      241 tcggccatct gcacggtggc cagccggtgg gcgatgatga tggcggttcg attcgccaac
      301 agacgctcca gcgccgcctg aatttgtctt tcggtttcgg tatcgaccga cgatgtcgcc
      361 tcatccatga tgaggatttt cggatcgtag gccagggccc gcgcgaaagc taaaagttgt
      421 ttctgtccca ccgaaagggt gctcccacgc tccgttacct cttcctggta cctccgggga
      481 aggttgcgga tgaaccggtc ggccccgact tcccgggccg cccattccac ccgttccaaa
      541 ggcatcgcct cgctgcccag gcggatgttc ccggcaatgt cgcccgaaaa gaggaacaca
      601 tcctgcagaa ccaaaccgat atttctgcgc agcgaggctt cgttgatcgt tttcaattcg
      661 gtcccatcaa tcaaaatcga cccgcgttgg taatcgtaga accggcagag gagtgaggca
      721 atcgacgtct tccccgcgcc ggtggcgccc accagcgcaa tcttttcccc cggtttgacg
      781 gaaaaactca cttcccggag gacgtactcc tcgccccggt aggcaaacca gatatttttg
      841 aattcaatgg cccctttgac cggagccagc tccttcgcgc ccggtttggc gctgaattcg
      901 ggctgggtat ctaacagttt gaaaatccgc tccgaggacg ccatcgccga ctggaggatg
      961 ttgtactttt cggccaagtc gcggatcggc tggtagaagc ggtccacgta ctggatgaag
     1021 gccaccagcg cccctaaggt gagaaacccg gagagcacct gccccccgcc gtaccagatg
     1081 atgagggcaa tcgagagcgc gccgatcaaa tcgaccgccg ggaagaagac cgcgtgatag
     1141 aagatcgaac gcaggtggga gtcgcgcagg gaagcgttga cgccgtcgaa ctggctgaaa
     1201 actttctttt cctgattgaa cacctgcacc gtggccatgc cggagatctg ctcctgcaga
     1261 aaggccataa tcttggccac cttcagccgc acttcgcggt aggcagcgcg tacctttcgc
     1321 cggaaaacca gcgtggcata gaataggagc ggaatcgcca caaacgccac gagagccagc
     1381 ttccaattta agtagagcat gatggcgaca atgcctgcga gcgtgaagag atcgccgaaa
     1441 accgccacca cgccggaggt gaacatttcg ttcagcgcgg cgacgtcgga agtgacgcgg
     1501 gtcaaaagcc ggccgaccgg atttcggtcg taaaacgaga gggtcaactt ctgcaggtgg
     1561 gagaaaatcc ccatccgcag gtcgaacatc gccttctgcc ccacccactg catcaggtag
     1621 gtctggacat agcggaccac gaactgcact gccagaatga ggatgaagag caaagccaga
     1681 ccattcagcc cgtgcagatt gccttttttg atatagtcgt caatggcaac cttggtcagg
     1741 tagggtgggg ctaactggag gagcgatacg gccagaagca gaaccacggc gat
//
LOCUS       DBNGZA010000347         4027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_812362, whole genome shotgun sequence.
ACCESSION   DBNGZA010000347 DBNGZA010000000
VERSION     DBNGZA010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_812362"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="AC16FU_07725"
     CDS             complement(<1..237)
                     /locus_tag="AC16FU_07725"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994293.1"
                     /translation="MHILKHVFRVGVLLLLYSPSSSFATNADCLGCHQEKDLTKTTAE
                     GSVQSLYVDQKKFENSVHGQLACVDCHADLAQAEF"
     gene            complement(260..1696)
                     /locus_tag="AC16FU_07730"
     CDS             complement(260..1696)
                     /locus_tag="AC16FU_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994294.1"
                     /translation="MKKLFARVSRNLLSFLGLLFALVSSVLVITIVAIDLLGVQISPY
                     IGILVYLILPAFLVAGLVMVPVGLALEKRRVHLQQGLPKELALPKIELDLNRPVLRKR
                     IAWLAVLSTLFLSILATATYKAVEFMESVTFCGQVCHKVMEPELVTYSHSPHSRVACV
                     SCHIGPGAPWFVKSKLSGVGQVFAVTFNTYDRPIPTPVKNLRPARETCEQCHWPQKFH
                     GQQLRVFTHYSADEKNTALKTVLLLKTGGGDKESGIAEGIHWHMNIANEISYIATDAA
                     RQTIPWVRLKDLKGKIVEYATPDFQVSRDSLAKLPQRVMDCMDCHNRPTHVYRLPDDA
                     VDEALLTRQIDPGLPYVKKAAVELLQSEYANKPEALQKIKSGLFTYYRQNYPVLADSL
                     ALEIQKAGEKLAQVYANNVFPKMKITWGTYRSNIGHQNSSGCFRCHDDNHQSKDGQTI
                     RQDCDQCHSLLATEEENPKPVIESLPIR"
     gene            complement(1693..2958)
                     /locus_tag="AC16FU_07735"
     CDS             complement(1693..2958)
                     /locus_tag="AC16FU_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994295.1"
                     /translation="MTLLKKILAASILGLTFSTPAGAKDNCLECHGQLDGNLQAPAVG
                     ITDDIHYQRGISCAGCHGGDSKEEDADLSMNPAKGYVGAPKPAQIPAFCGRCHSDVEY
                     MKRYDPRLPTDQYAQFKTSVHGKRLVRGDTLVANCVSCHGVHGILPPSDTRSPVFKTH
                     IPGTCAHCHADTKRMASYGIPTNQYDDYRQSVHGELLLDKGDRSAAACTDCHGNHGAT
                     PPGLSDVAGACGECHPANRDLFEKSPHKIPFARTGLPQCATCHDNHKVLRPTDAFLGT
                     GPEAVCIRCHSEGDKGYSSAEEFKASLDSLKSAVAEARGLLERAEVAGVKTALAKFDL
                     KASEDAVIRSRSALHSFSLADFQAITRDGLGRALQVKKAALASLEDLEIRRKGLAFSA
                     VVILLLCIGLWLKIRRIEARQKEKAGRTQ"
     gene            complement(2963..>4027)
                     /locus_tag="AC16FU_07740"
     CDS             complement(2963..>4027)
                     /locus_tag="AC16FU_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR2994296.1"
                     /translation="GFYRWLGERINLQPLLHLVKKKAVPAHRHSVWYYTGGMTLFFFA
                     VQVATGILLLLYYRPSAEAAYESVQFIMTEVKFGWLVRSIHSWSANLMIGAIFVHMFS
                     TFFMKAYRKPRELTWFSGASLLFLALCFGFSGYLLPWNKLAFFATKVGTEIAGQLPGI
                     GHSLLVFLRGGEEVSGATLTRFFGFHVAILPGVVTFLLLLHLLLVQVQGMSVPIRVAK
                     NPTYRTVPFFPNFLLRDLLGWLLALLLLVVLASIFTWELGEKADPLAPAPAGIRPEWY
                     FLFMFQTLKLIPAKILSIPGEFLGILAFGAGGILWFLVPLLDKSPEGKSGRWLFRLGW
                     VLVAYMAVLTVLGYLWPAGK"
BASE COUNT          893 a         1153 c         1178 g          803 t
ORIGIN      
        1 gaactccgcc tgcgccagat cggcgtggca gtcaacgcac gccaattgcc catgaacgga
       61 gttttcaaat ttcttctggt caacgtagag ggattggacg ctcccctcgg cggtcgtttt
      121 cgttaaatcc ttttcctgat ggcacccgag gcagtccgcg ttggtggcaa aagaactgga
      181 gggcgagtat aagagtaaaa gtaccccgac tcgaaaaaca tgttttagaa tgtgcaccga
      241 ctactccgac atcgcaactc taccttatcg gtaaagattc aatcaccggc ttgggatttt
      301 cctcttccgt cgccaaaaga ctgtggcact ggtcgcagtc ctggcggatg gtttggccgt
      361 ctttgctctg gtggttgtcg tcgtggcagc ggaagcaccc ggatgaattt tggtgcccaa
      421 tgttacttct ataagtcccc caggtaatct tcatcttggg gaaaacgttg ttcgcataga
      481 cctgggctaa cttctccccg gctttttgaa tctccagtgc cagactatcg gccagcaccg
      541 gatagttctg tcgatagtaa gtgaaaagac cgctcttgat tttctgcaac gcttccggct
      601 tgttcgcata ttccgactgc aaaagctcca ccgccgcttt cttgacatag ggcagcccgg
      661 gatcgatctg ccgggtcaac agcgcttcat ccacggcgtc gtccggcaag cggtacacat
      721 gggtgggccg gttgtggcag tccatgcagt ccatcacccg ctggggcagt ttcgccaggg
      781 aatctcggga cacctgaaag tcgggagtgg cgtattcgac gattttgcct ttgaggtctt
      841 tcaggcgcac ccagggaatg gtctgccgcg ctgcatccgt ggcgatgtag ctgatctcgt
      901 tggcgatgtt catgtgccag tggatcccct ccgcaatgcc ggattctttg tctccgcccc
      961 cggtcttaag caggagcacg gttttcaagg ccgtattctt ctcgtctgcg gaatagtggg
     1021 tgaacacccg cagttgctgc ccgtggaact tttggggcca gtggcactgc tcgcaggttt
     1081 cccgcgccgg gcgcaggttc ttcaccggcg tgggaatggg gcggtcgtag gtgttgaagg
     1141 tcaccgcgaa cacctgcccc acgccggaaa gtttgctctt gacgaaccag ggcgcgcccg
     1201 ggccgatgtg gcagctgacg caggcgaccc gggagtgcgg ggaatgcgaa taggtgacga
     1261 gctccggctc catcaccttg tggcacacct gcccgcagaa ggtcaccgac tccatgaatt
     1321 ccaccgcctt gtaggtggcc gtggccagga tcgaaaggaa caaggtggag aggaccgcga
     1381 gccaggcaat tcgtttgcgc aagaccggcc ggtttaaatc gagttcgatt ttcggcaagg
     1441 ccagctcttt gggcaggcct tgctggagat gcacccgcct tttctccaaa gccaacccca
     1501 ccgggaccat gaccaaaccg gccaccagga acgccggcag gatgagatag actaaaatgc
     1561 cgatgtaggg actgatctgg acgcccaaaa ggtcaatcgc cacgatggtg ataaccagaa
     1621 ccgaagagac taaagcgaaa agcaaaccga gaaaggagag gagattgcgc gagacgcggg
     1681 cgaaaagttt cttcattgcg tccggcccgc tttctctttc tgccgggctt cgatccggcg
     1741 gattttcaac cacagcccga tgcacagaag caaaatcacc accgccgaaa aagccaatcc
     1801 tttgcggcgg atctccaggt cttccagcga cgccagagcc gcttttttca cctgcagggc
     1861 ccggcccagt ccgtcacggg tgatcgcctg aaaatcggcc agagaaaaag agtgcagggc
     1921 tgagcgggag cgaatgacgg catcttcgga cgccttcaag tcgaatttgg ccagggcggt
     1981 tttgaccccc gccacttcgg cccgctccag aagccctcgg gcttcggcca ccgcgctctt
     2041 gagcgaatca aggctcgcct tgaactcttc cgccgaactg tagcccttat ccccctcgct
     2101 gtgacagcgg atgcaaaccg cttccggccc cgtaccgagg aatgcatcag ttggccgcag
     2161 cactttatga ttatcgtggc aggtggcgca ctgcggcagg ccggtccggg caaagggaat
     2221 tttgtgcggc gatttttcaa aaagatcccg gttggccgga tggcactcgc cgcaggcccc
     2281 ggccacgtcg gaaagccctg gcggtgtggc cccgtggttg ccgtggcaat cggtacaggc
     2341 cgccgccgac cgatcgcctt tatccaagag caattctccg tgtacgctct gacggtagtc
     2401 gtcatactgg ttggtgggaa tgccgtagga tgccatccgc ttggtgtcgg cgtgacagtg
     2461 tgcgcaggta ccgggaatgt gcgttttgaa caccggtgag cgggtgtctg agggtggcaa
     2521 aatcccgtgc accccgtggc aggaaacgca gttggccacg agggtatccc ctctgactaa
     2581 ccgctttcca tgcacggagg ttttgaactg agcatactgg tcggtgggta gccgggggtc
     2641 gtaccgcttc atatactcga cgtccgaatg acagcggcca cagaaggcgg ggatttgtgc
     2701 cggcttgggc gcgccgacgt accctttggc cgggttcatc gaaaggtctg cgtcttcttc
     2761 tttcgaatcg cccccgtggc agccagcgca cgaaatcccc cgctggtagt gaatgtcatc
     2821 ggtgatgccg acggctggcg cctggagatt gccgtctaat tgcccgtggc actccagaca
     2881 gttatccttc gctcccgcgg gagtggaaaa cgtcaagccc aaaatgctgg ctgcgagaat
     2941 ttttttcagc agagtcattt ttttatttcc ccgccggcca gagatacccc agaaccgtta
     3001 agacggccat ataggcaacc agaacccagc cgagccggaa aagccaccgc cccgattttc
     3061 cttcgggaga tttatccagg aggggaacca aaaaccacag aattcccccc gcaccaaagg
     3121 ccaaaattcc taaaaactcc ccggggatag ataagatttt ggccggaatc aacttgaggg
     3181 tttgaaacat aaagaggaag taccattccg gccggatgcc cgccggcgcc ggagccagag
     3241 gatccgcctt ctcccccagt tcccaggtga aaatggaagc cagaacgact aagagcagaa
     3301 gcgccaaaag ccagcccaac agatcgcgca gaagaaagtt tgggaaaaaa ggaacggtgc
     3361 ggtatgtcgg attcttggcc acccgtatgg ggacgctcat accctgcacc tgcaccagca
     3421 ggagatgcag gagcaaaaga aaggtgacca ctccgggcaa aatggcgacg tgaaagccga
     3481 aaaagcgcgt cagggtggcg ccggaaacct cttccccgcc ccgcaggaaa accaagagac
     3541 tgtggccgat tcccggcaac tgcccggcga tctcggtgcc gactttggtg gcaaagaagg
     3601 ccagtttgtt ccagggcaat aggtagccgg aaaaaccgaa gcagagcgcc agaaagagaa
     3661 gagaggcgcc cgagaaccag gtcagctcgc gcggcttgcg gtaggccttc atgaaaaatg
     3721 ttgagaacat atgcacgaag atggcgccga tcatcaagtt ggccgaccag gagtgaatcg
     3781 aacgcaccag ccacccaaac ttcacttcgg tcatgatgaa ctgcaccgat tcgtaggccg
     3841 cttccgcgga ggggcggtag tagagcaaga gcagaatgcc ggtcgccacc tgcaccgcga
     3901 agaaaaagag tgtcatcccg ccggtatagt accagaccga atgccggtgg gccggcaccg
     3961 ctttcttttt gaccagatgc agaagtggct ggaggttgat ccgctccccc agccaccggt
     4021 aaaagcc
//
LOCUS       DBNGZA010000348         4156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_492435, whole genome shotgun sequence.
ACCESSION   DBNGZA010000348 DBNGZA010000000
VERSION     DBNGZA010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_492435"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..3584)
                     /locus_tag="AC16FU_07745"
     CDS             complement(222..3584)
                     /locus_tag="AC16FU_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR2994297.1"
                     /translation="MRIRHTLFLGAVGFFVLFAFVSSYLWAGNTGKIVGRVVDKETKE
                     GVVGATVRVDGTSLGAASDAEGKFFILSVPPGKYRVTITGVGYISLGVTNVTVVTDQT
                     TDLGTLKVEPSAIEMPAQKVVGAPPLINPNVTANVRNLSAEDIKNLPVKTVEDLLRLQ
                     VGVTKQGDVIHVRGGRGNEILYVVDGVEVRDPLGGRGPVGQALNVASTSIEELSIIKG
                     GFDAEYGNAQSAVVNISTKEGEVKTTRGHIEYFTDDFGSSSLDKYSYNFDRLEASLSG
                     PDPFLTTRFLPALGISGLEDKLTYFLSADADKTDTYLSFAKLASPTTQPTFRNYRFLG
                     LNIPDRQQNSYTTNMKITYKATPNLRFALNYKGSWDRYHNYQPGAGAGGTGGWYYRYS
                     PFTQALNREWGSVYSLNATHNVSKNTFYQVVLSRFFRRYFEAPSDPNRPGEVLLPNQF
                     RLADTQYEVYDDLNHNGHWDDPEPFTDLNHDGRYTFGDVFDDQNGNGKYDPGIDILLW
                     DWNRNGQYDQTEGEPFVDLNGNGVRDAGDNLRVDLNGNGRYDDRYRPVQSWGGSFADR
                     PEPYQDGDISLGEPFEDRNHNGVFDPGVDEQKICACPENQDLNRNSKYDGPKDPWTPG
                     VPYLDVNHNGRYDPPNKRYDRGEAFVDVNGDSKWNPTDLFLDPGTFEIRGIYEQRTVT
                     LTTAAFDITSQFKKSHEVKTGLKLEKNRLTMQDIRYVEVPYDGPPDPTQPFNDRGIFR
                     DFYDRSPIRLATYVQDKIEYGALIARLGLRYERFLQANLNDLRKEREDAGLIDTVIAR
                     NRSKLAPRMGFSYPITERAKIYFNYGHFYQLPEFQYFYRRSTQGSLAFGIIGNFNLDF
                     SKQISYEFGVNIALSNSYKLDVSGFFKDYFGQINTLVQRNGPIERDIYANLDYARTRG
                     FELELDKKYGNYISGYVNYQFAYAFGKASTESQNYNDRFNNRQIPIKENPLSWDQRHQ
                     ITFNLDLRIPRKERPRIFGLRVPDNWGINVVWQYGSGFPFTPTRTFPGLRVPPGEQPQ
                     VNSMRIPSSSQVDFRFNKDFEVWKLNYSFEVWVNNVFNRQNISSVYTNTGFADTCNQP
                     SSSAIEPGTDYQRRPDYFGPGRNVRLGLALNF"
     gene            complement(3753..>4156)
                     /locus_tag="AC16FU_07750"
     CDS             complement(3753..>4156)
                     /locus_tag="AC16FU_07750"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR2994298.1"
                     /translation="YVMKAACFASQTQGEIAVGKMKVVGSAQRQVGGRFLQHGSVPLA
                     PPRVAIEDLFGVELPEKNRSSMTLTEASGRPVGYREVADALKASFAERFAVTWQEAGL
                     TPAERQTAGRLAEERYRRMDWSGQNQKLAVV"
BASE COUNT          790 a         1203 c         1224 g          939 t
ORIGIN      
        1 aaactgaccg ccagcccggc ccaaaaacta ccgcgctttt cttttttcaa tcgcatggaa
       61 aaagcctcct catcttgaac tctgaaataa cctgttttcc gcccgcattg tttcgccttt
      121 ttgtaacagt gcctttcact caaagaaacc ctgctttggc tcggtagcct agccctttat
      181 gggcgtccaa ggccgacaag cggctatgtt acaaaaccct tttagaagtt caacgccaat
      241 cccagccgga cattccggcc aggaccgaag taatcaggcc gcctctggta atcggtgccc
      301 ggttcaatcg ccgaactcga cggctggtta caggtatcgg caaagccggt gttggtgtaa
      361 acagagctga tgttctgccg gttgaagacg ttattgaccc acacttcaaa cgaatagttc
      421 agcttccaga cttcgaagtc cttgttgaag cggaaatcga cctggctgga agaaggaatc
      481 cgcatggagt tgacctgcgg ctgttcgccg ggcggcactc gcagcccggg gaaggtgcgc
      541 gtgggagtaa aggggaaacc ggagccgtac tgccagacca cgttgatgcc ccagttgtcc
      601 ggcacccgga ggccgaagat gcgcgggcgc tccttccggg ggatccgcaa atccaaattg
      661 aacgttatct ggtgcctctg gtcccaggag agcgggttct ccttgatcgg gatttgacga
      721 ttgttaaagc ggtcgttgta attctgcgat tccgtggagg ccttgccgaa ggcataagcg
      781 aactggtagt tgacgtagcc ggagatgtag ttgccgtact tcttatccag ttcgagttca
      841 aagccccggg tccgggcgta gtctaagttg gcgtagatgt cccgctcgat ggggccgttg
      901 cgctggacta aggtattgat ctgcccgaag tagtctttga agaagccgga aacgtccagt
      961 ttgtaactgt tggagagggc gatgttgacg ccgaactcgt aggatatctg cttggagaag
     1021 tcgagattga agttcccgat gatgccgaag gctaaagacc cctgggtgga ccgccggtag
     1081 aagtactgga actccggcag ctggtagaag tgtccgtagt tgaagtagat tttggcccgc
     1141 tcggtaatgg ggtaggaaaa acccatccgc ggggccagtt tggaacggtt gcgcgcaatg
     1201 acggtgtcaa tcaagccggc atcctcgcgc tctttccgca ggtcgttcag gttggcctgc
     1261 aggaaccgtt cgtagcgcag ccccagtcgg gcgatcaagg cgccgtattc gattttgtcc
     1321 tgtacgtagg tggccaaacg gatcggactc cggtcgtaga agtcccggaa aataccccgg
     1381 tcgttgaagg gctgggtggg gtcgggcggc ccgtcgtagg gcacctccac gtagcggatg
     1441 tcctgcatgg tcagccggtt cttctccagc ttgaggccgg ttttcacttc gtgcgatttc
     1501 ttgaactgcg aggtgatgtc gaaagcggcc gtggtcaaag tgacggtccg ctgctcgtaa
     1561 atcccgcgga tttcaaacgt tcccgggtca aggaacaggt cggtggggtt ccatttgctg
     1621 tcgccgttca cgtcgacgaa cgcctcaccc ctgtcgtagc gcttgttggg gggatcgtag
     1681 cggccgttgt ggttgacgtc gaggtagggc acgccgggtg tccacgggtc tttggggccg
     1741 tcgtacttgc tgttgcggtt cagatcctgg ttttccggac aggcgcagat cttttgctca
     1801 tcgacacccg gatcgaatac gccgttatgg ttacggtctt cgaacggctc gcccagactg
     1861 atgtcgccgt cctggtacgg ctcaggccgg tccgcaaagc tcccgcccca gctctggacg
     1921 ggccggtacc ggtcgtcgta gcggccgttg ccgttcaggt caaccctgag gttgtcgccc
     1981 gcgtcccgca caccgttgcc gttcagatcc acgaacggct ccccctcggt ctgatcgtac
     2041 tgcccgttgc ggttccagtc ccataggaga atatcgatgc cggggtcata cttcccgttg
     2101 ccgttctgat cgtcgaaaac atcgccaaag gtatatctcc cgtcgtggtt taagtcggtg
     2161 aacggttcgg ggtcgtccca gtggccgttg tggttcaagt cgtcgtacac ttcgtactgg
     2221 gtgtcggcca ggcggaactg gttcggcaaa agcacttcgc ccggccggtt ggggtccgac
     2281 ggggcttcga agtaccgccg gaagaagcgc gacaagacca cctggtaaaa agtattcttg
     2341 ctcacgttgt gggtggcgtt caagctatag acactccccc actcccggtt gagcgcctgc
     2401 gtgaaaggcg aatagcggta gtaccaccct ccggtgccgc ccgcgccggc gccgggctgg
     2461 tagttgtggt agcggtccca ggagcccttg tagttcaagg caaaacgcag gttcggcgtg
     2521 gccttgtagg tgatcttcat attggtcgtg taggaattct gctgccgatc ggggatgttc
     2581 aagcccagga acctgtagtt ccggaaggtc ggctgcgtgg tgggtgaagc gagcttggca
     2641 aaggagaggt aggtgtcggt cttgtcggca tcggcggaga ggaagtaggt caatttgtcc
     2701 tctaagccgg aaatgcccaa agccggcaaa aagcgcgtgg tcaggaacgg atcgggaccg
     2761 gagaggctgg cctccaaccg gtcgaagttg taggagtact tgtccaggct gctcgagccg
     2821 aaatcgtcgg tgaagtactc tatgtgcccc cgggtggttt tgacttctcc ttctttggtg
     2881 ctgatgttca ccacggccga ctgcgcgttg ccgtactcgg cgtcgaaacc gcctttgatg
     2941 atcgagagct cttcaatcga ggtgctggcg acgttcaagg cctgccccac cggcccgcgg
     3001 ccgcccaagg ggtcgcggac ttccacgccg tccaccacgt agaggatttc gttgccccgg
     3061 ccgccccgga cgtggatcac gtcgccctgc ttggtcaccc ccacctggag ccggagcaaa
     3121 tcctccaccg tcttcaccgg caggtttttg atgtcctcgg ccgaaaggtt ccgcacgttg
     3181 gcggtcacat tggggttaat gagcggcggc gcccccacta ccttctgcgc cggcatttcg
     3241 atcgccgagg gttcgacttt caaagtcccc agatcggtcg tctggtcggt taccacggtg
     3301 acattggtaa cccccaggga gatgtaaccg acgccggtaa tagtgacacg atattttccg
     3361 ggcgggacgc tcaggatgaa gaattttccc tccgcgtcgg aggccgcgcc taagctggtg
     3421 ccatccacgc ggacggtggc gcccaccact ccttctttgg tctctttgtc taccacccgg
     3481 ccgacgatct tcccggtgtt cccggcccag aggtatgagg aaacgaacgc aaacaggaca
     3541 aaaaatccca ccgcccccag aaacaacgtg tgacgtatcc gcattgctct catcctcctt
     3601 caaacgaggc gtttattttg caactttctt tgggccaatc ccgtaaaaga aaagtttgac
     3661 gaccatcggc taatctgctg accttcccgt attttgtcaa gtcctttttt ggccacagaa
     3721 cgcgcggagg cttgttggac ggcccttctt tctcagacaa ccgccagctt ttggttttgc
     3781 ccggaccaat ccatacggcg atagcgttcc tcggccagcc gaccggccgt ctgccgctcc
     3841 gccggagtca atccggcctc ctgccacgtc acagcaaatc gttccgcaaa acttgccttc
     3901 agcgcgtcag ccacttcgcg gtagcccacc ggacgtccgg acgcttcggt cagggtcatg
     3961 ctcgaccgat ttttctccgg caactcgacc ccgaacaagt cttcaatcgc gaccctcggc
     4021 ggtgccaaag gtaccgaccc gtgctgtaaa aacctgccgc ctacctgccg ttgcgcgctg
     4081 cccacaacct tcattttccc gacggcgatt tctccctgcg tttgggacgc aaagcaggcc
     4141 gccttcatca cataac
//
LOCUS       DBNGZA010000349         2605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_915300, whole genome shotgun sequence.
ACCESSION   DBNGZA010000349 DBNGZA010000000
VERSION     DBNGZA010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_915300"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="waaF"
                     /locus_tag="AC16FU_07755"
     CDS             complement(<1..1075)
                     /gene="waaF"
                     /locus_tag="AC16FU_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR2994299.1"
                     /translation="MSARSLEPTKIKKIVVRAPNWVGDVVMSLPFFAQLRDIFLYAEI
                     SCLVKSHLAELYQHQPFISRVIPLAERANRLAVIFKTSQEIRRKKFDLAICLPNSFSS
                     AMIFFLAGIPRRVGYAAEGRGLLLTDRFPYLPDKKMIHQSEIYTNLLRAFGFQSDSDY
                     LPVLTAPPSVQKEVDRLFEKHRFTQKQRLAVIAPGSRAPARQWFPNRFAKLADRLIAE
                     GQTVLFVGGPADVPTAVEVKAKMKQKPIDLSGQTSLLGLAEVCRRSRVFIGNDSGAAH
                     VAAAAGTHVVVITGPGDPEQITPLTNRKTVLFHKIFCSHCRKNHCWRSDLPQECLRLI
                     SVDEVLETVWPILESTKVPQKIQA"
     gene            complement(1072..>2605)
                     /locus_tag="AC16FU_07760"
     CDS             complement(1072..>2605)
                     /locus_tag="AC16FU_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994300.1"
                     /translation="GMSKISVASSQGPVARKKSRQTLGQCLNATLVIALLLVVPLFAA
                     IAQEPVKLTLPECIALAMKQGKEAQIARSELEAAKSTYQARRLGYYLPRVNFQLTTPS
                     YQNSQSLQNFFGATERVLSKSTSLYFNGGVVLSQNLLTGGTLEVTGRANSLRNTANIF
                     SDFRERSGELLLSLNQPLFAASPHRNDLHQAEFAEKRAQWQYHERVWGLYRDVMQAYV
                     DLLAAGIEADVAGLELEKARMAARDAEEKKTQNLVDEKEVLTARAKTLEQEVVQLDAL
                     RKREEKRLALFGLLGMKSEAAVELSGQIVPEAVPADRLEWYRSNASLTLDAKRAALSV
                     DEKKTALEQTRSQGGLGGTLSGSADIGRGKEKRDVTNNINTNRYGVSLTLTYPLWDGG
                     AQAAAERAAEVAYETVKLDREKQIQAAVTKVTSAINKVTTGQKRVELLGRIAELERQR
                     LARAEEQQGQGLLSAKALADLKISALDAQTKYLQAVADHNAAVYELHQSFLEDTPPEI
                     PR"
BASE COUNT          509 a          753 c          726 g          617 t
ORIGIN      
        1 ctgcctgtat cttttgcggt acttttgtgg actcaaggat tggccacact gtctccagca
       61 cttcgtcaac ggaaatcagg cgcaggcact cctggggcag gtcactcctc cagcagtgat
      121 tcttccggca gtgtgagcaa aatattttat gaaagagaac cgtcttgcgg ttggtgagcg
      181 gggtgatttg ttctggatca cccggaccgg tgatgaccac cacgtgcgtc ccggctgccg
      241 cggccacgtg tgcggcgccc gagtcgttgc cgatgaaaac ccggctgcgc cggcaaactt
      301 cggccagacc caaaagcgag gtctgcccgg agaggtcgat gggcttctgc ttcatcttcg
      361 ctttcacttc caccgcagtg ggaacatccg ccggcccgcc cacgaagagt accgtttgtc
      421 cttctgcgat cagccgatca gccagttttg cgaatcggtt gggaaaccac tgccgcgcgg
      481 gcgcccggga gccgggggcg attacggcca gccgttgctt ttgggtgaac cggtgtttct
      541 cgaagagacg atcaacttct ttttggacgg agggaggggc ggtcagaacc gggaggtaat
      601 cgctgtctga ctggaatcca aacgctcgca gtagattcgt gtaaatttca ctctggtgaa
      661 tcatcttctt atccggcagg taagggaacc ggtcagtcag gagtaaaccg cgtccctcgg
      721 cagcataccc tacccgcctg gggatgcccg ccagaaagaa gatcatagcc gacgaaaacg
      781 aattgggcag acagatggcc agatcaaatt tcttgcgacg aatctcctga gaagtcttga
      841 aaatcacggc caatcgattg gcccgctcag ctaaaggaat cacgcgagaa ataaacggtt
      901 gatgttggta aagctcagcc aaatgtgatt tcactaagca gctaatctcg gcgtagagaa
      961 agatgtcccg cagttgggca aagaaaggaa gcgacattac cacgtcgccc acccagttcg
     1021 gcgcgcgcac aactattttt tttatcttcg ttggttcgag tgagcgggca ctcaccgtgg
     1081 gatttccggt ggggtgtctt cgaggaaact ttgatgcagt tcgtacaccg cggcgttatg
     1141 gtcggccacc gcctgcaggt acttggtctg cgcgtcgagg gcgctgattt tgaggtcggc
     1201 caaggccttg gctgagagaa gcccctgccc ctgctgctct tcggcccgcg ccagacgctg
     1261 gcgttccagt tcggcaatgc ggcccaatag ttccactctc ttttgtccgg tagtgacttt
     1321 atttatcgcg ctggtcactt tggttacggc tgcttgaatc tgtttttcgc ggtcgagttt
     1381 caccgtctcg tacgccacct ccgccgcgcg ttcggccgcc gcctgggcgc caccgtccca
     1441 gagcgggtag gtcaaggtca aagacacgcc gtaccggttc gtgttgatat tgttggtaac
     1501 atcccgcttt tcctttcccc gcccaatgtc agccgagccg ctgagcgttc cgcccaaacc
     1561 gccctgcgaa cgggtttgct ccagagccgt tttcttctcg tctacggaaa gagctgcgcg
     1621 cttggcgtcg agagtcagcg aagcattgct ccggtaccac tccagccggt cggcgggaac
     1681 ggcttccggt acaatctgac cggataattc aacggccgct tcgcttttca tacccagcag
     1741 accgaacagc gccagccgct tttcttccct tttccggagg gcgtccagct gaactacttc
     1801 ctgttccagt gttttggctc tggcggtaag cacctctttt tcatccacca agttctgcgt
     1861 ctttttttct tcggcgtcgc gggccgccat tcgtgccttt tctaattcca ggccggccac
     1921 atccgcttca atgcctgcgg cgagaaggtc aacatatgcc tgcatcacgt cgcgatacag
     1981 accccatact ctctcgtggt attgccactg cgcacgtttt tccgcgaact ccgcctggtg
     2041 caggtcattt cggtgcggcg aagcggcaaa cagcggctgg ttcaaggaga gcaggagctc
     2101 tccgctccgt tcgcgaaagt cggagaagat gttggccgtg ttgcggagcg aattggcccg
     2161 gccggtgact tccagggtcc cgccggtcaa gaggttctgc gaaagcacca ccccgccgtt
     2221 gaaatataaa ctggtgctct tggagagcac ccgctccgta gcgccgaaga aattctgcaa
     2281 actctgactg ttctgatagg aaggagtcgt taactggaaa tttactctgg gaagataata
     2341 ccctaaccgc cgtgcctggt aggtcgattt ggccgcttcc aattcgctcc tggcaatttg
     2401 cgcttccttg ccctgcttca tggccagagc gatgcattcg gggagcgtta gtttgaccgg
     2461 ctcctgtgca attgccgcaa aaagtggaac caccaggagt aacgcaatta ccagcgttgc
     2521 attgaggcat tgtcccaatg tctggcgact cttctttctg gctaccggcc cctggctact
     2581 ggctaccgaa attttactca taccg
//
LOCUS       DBNGZA010000350         2717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_171179, whole genome shotgun sequence.
ACCESSION   DBNGZA010000350 DBNGZA010000000
VERSION     DBNGZA010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_171179"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16FU_07765"
     CDS             complement(<1..235)
                     /locus_tag="AC16FU_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994301.1"
                     /translation="MRKFFVVVSLVVPMAFLTGLSSAWAHTPLTNWLQDPATPLAREV
                     RADFWTMLAIIAPFLLGAEGLLLYAVLKFRAKPG"
     gene            complement(232..1197)
                     /locus_tag="AC16FU_07770"
     CDS             complement(232..1197)
                     /locus_tag="AC16FU_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2994302.1"
                     /translation="MEKPKEKTNPFYYFPFFILAAVLVVFVFLSGYQRGVSTGQRTPP
                     PLEKPGGQVATVDIRKLRIATPEALARGKMLFLTNCVSCHGPEGRGDGERAAELSPRP
                     RNYHTEKFKFGAAPIQIYHTISNGSPGTSMPAFELLPPEDRWALVHFVRTQIPNPPDD
                     SPEEIAKLPGGEGGPASVTTTPVTQPTVPIPAAPRIPIALAMQKLAVPPPEPGEKLAL
                     PAHGQGAVIYNRNCVTCHGGSGEGGRKTIYLLRLKPYLYVKSKSLQEPDRPWRQNKNE
                     FMKIVTKGFPGQTMPGFGTLTRSEIEALYAYVLNFAAPKKNGNKS"
     gene            complement(1277..2494)
                     /locus_tag="AC16FU_07775"
     CDS             complement(1277..2494)
                     /locus_tag="AC16FU_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HIR2994303.1"
                     /translation="MSEPVVTAVVNPGTLPVSVRLRGVLLACTLVGIVTFAIALKVDT
                     QRAWYNFLQEHYFFLCLGLAGVFFVALQHATNSTWSVSLRRIPEGLAAYLPVALLLQI
                     GVIIGAKYLYAWVTPEFTEAHPLHYAGQPPPGGRLAYLNLPFFSIRTFAFLGVWILFG
                     WLLVRNSIQQEETRDPLLSRKNVKLSIAFLPVFALTFTLAVVDLLMSLEPRWYSTIFG
                     VYCFSGLFQGGLAFIIILVILLRRQGALAGVTSPAHLKDLGTLLFAFSVFMCYIGFSQ
                     FMLIWYTNLPEETFYIMKRIANGWLPVALALPLLKFFFPFFVIMSQNAKKNEKVMLVT
                     AIVVLVGQWLDVYWMVAPAFRQGPPLFGWIEAGIAIGFLGLFGLCLVTFFRRFSLVPA
                     GDPRLLESVHLHH"
     gene            complement(2507..>2717)
                     /locus_tag="AC16FU_07780"
     CDS             complement(2507..>2717)
                     /locus_tag="AC16FU_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2994304.1"
                     /translation="HIITRGRGNMPSYATQLDPTDRWAIIHYVRVLERAAAPTPEDLA
                     EMRQKGFDFSEDEPDTTSAGYWPKK"
BASE COUNT          613 a          736 c          849 g          519 t
ORIGIN      
        1 gtccgggctt agcgcggaat ttcaaaacgg cgtagagcag aagtccctcg gcacccaaaa
       61 ggaaaggggc gatgatggcg agcatcgtcc agaagtccgc ccgcacctcg cgggccaaag
      121 gcgtggccgg atcttggagc caattggtca atggtgtatg cgcccacgcg ctgctaaggc
      181 cggtgaggaa cgccatcgga acgaccaggg ataccaccac gaaaaatttt ctcatgactt
      241 attcccgttt ttctttggtg cggcaaaatt cagcacgtag gcgtagagcg cctcgatctc
      301 cgaacgggtc agagttccaa acccgggcat ggtctgtccc ggaaatcctt tggtcactat
      361 tttcataaat tcatttttgt tctggcgcca gggacggtcc ggctcctgca aacttttgga
      421 tttgacgtag aggtagggtt tcagccgcaa aaggtagatc gttttccggc cgccttcgcc
      481 cgatccgccg tgacaggtga cgcagttccg gttgtagatt acggcgcctt ggccgtgagc
      541 gggaagcgca agtttctctc ccggttccgg aggaggcacc gccaatttct gcatcgccag
      601 cgcaatgggg atgcgcggag ccgcaggaat tgggaccgtc ggttgcgtca ccggagtcgt
      661 tgtgactgag gccggcccgc cttctccgcc gggtaatttg gctatctctt ccggggagtc
      721 gtcgggcgga ttcgggattt gcgtccggac gaagtgaacc agcgcccagc ggtcttccgg
      781 cggtaatagt tcaaaagcgg gcatcgaggt gccgggtgag ccgttggaaa tcgtgtggta
      841 aatctgaatc ggcgcggcgc cgaacttgaa cttctcggta tggtaattcc gcggacgggg
      901 tgaaagttca gcggcccgtt cgccatcgcc tctcccctcc ggcccgtggc aggaaacgca
      961 gttggtcaaa aagagcatct tccctctggc cagcgcctcc ggggtggcga tgcgcagttt
     1021 gcgaatatcc accgtcgcca cctgaccccc gggcttttcg agcggcggcg gcgtgcgctg
     1081 gccggtactc acgccccgct ggtagccgga gaggaaaacg aagacgacca aaacagcggc
     1141 taaaatgaaa aaggggaaat agtagaacgg gttcgttttt tcttttggtt tttccattac
     1201 gcggttttta ccttctcttt actccattgt aagtacgcct ttatggcgat tgtgatagtc
     1261 ttctttctca ctttaatcaa tggtgcaaat ggacactttc caggagccgc gggtcgcctg
     1321 ccggcaccaa agaaaagcgg cggaaaaagg tcaccagaca caagccgaat aaccccaaaa
     1381 agcctatggc gatgcccgct tcgatccagc cgaagagcgg tggtccctgg cgaaacgccg
     1441 gcgccaccat ccagtaaaca tccaaccact ggcccaccag caccactatg gccgtgacca
     1501 gcatcacctt ctcgtttttc ttggcgtttt gtgacatgat gacgaagaag gggaaaaaga
     1561 atttcaaaag cggcagcgcc agggcgaccg gcagccagcc gttagcgatg cgtttcataa
     1621 tgtaaaacgt ctcctcgggc aaattggtgt accagatcag catgaactgg gaaaatccga
     1681 tgtagcacat gaagaccgaa aaagcaaaaa ggagggtgcc caagtctttc aggtgggccg
     1741 ggctggtgac gccggccaga gcgccctggc gccgcaggag aatgaccagg atgatgatga
     1801 aggccagccc gccctggaag agcccggaaa agcagtagac cccaaagatg gtggagtacc
     1861 agcgcggttc cagagacatc aagaggtcga ccacggccag ggtgaaggtg agagcgaaga
     1921 cgggcagaaa ggcgatggag agtttaacgt ttttacggct caagagcgga tcgcgcgttt
     1981 cttcctgctg gattgaattg cgcaccaaaa gccagccgaa gagaatccag accccgagga
     2041 aggcaaaagt ccggatggag aagaagggca agttcaggta agccaggcgg ccgccgggcg
     2101 gcggttgacc cgcgtagtgg agggggtggg cttcggtgaa ctccggggtg acccaggcgt
     2161 agagatattt ggccccgatg attactccaa tctgcaggag gagcgccacc ggcaggtagg
     2221 cggccagccc ctccggaatg cggcgcagcg aaaccgacca ggtggagttc gtggcgtgct
     2281 ggagggcaac gaagaagacg ccggccagcc ccagacagag gaagaagtag tgttcctgca
     2341 ggaaattgta ccaggcgcgc tgggtgtcaa ctttcagggc gatggcaaaa gtgacgatcc
     2401 cgacgagggt gcaagccaag agcaccccgc gcaggcggac cgacaccggc aaggtacccg
     2461 gattaactac cgcggtaacg acgggttcac tcatagagcg actgtttcac ttcttgggcc
     2521 agtaaccggc cgaggtggta tccggctcgt cttcagaaaa atcgaatcct ttttgcctca
     2581 tttcggcgag atcctccggc gtgggggcgg cggcccgctc cagcacccgc acgtagtgaa
     2641 tgatggccca gcggtcggtc ggatccagct gcgtggcgta ggaaggcatg ttcccccggc
     2701 cgcgggtgat aatgtga
//
LOCUS       DBNGZA010000351         1132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_812729, whole genome shotgun sequence.
ACCESSION   DBNGZA010000351 DBNGZA010000000
VERSION     DBNGZA010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_812729"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="AC16FU_07785"
     CDS             complement(<1..910)
                     /locus_tag="AC16FU_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR2994305.1"
                     /translation="MRVRPLKFVVPLLLALVVFLSLCEPASAQKLLIPMDLSQADHLK
                     AYGIAFWCLQNAHNVEWLLNYRGGSFLTDGWQALADLCRLRGVTFTPIDAAGAGQIYQ
                     TIEQGNMDVVLLEKAPKLAVYVPANREPWDDAVRLALDYAEIPYTTLWDTEVLRGDLA
                     KYDWLHLHHEDFTGQYGKFYGAYRNALWYREEVATNEALAHALGFRKVSEEKKAVART
                     IKDYVASGGFVFAMCSAPETIDISLAADKDDIVAAEFDGDPPDPDYQEKLDYSQCLAF
                     ENFVVYPDALDNRRSNIDTITDRLRYI"
BASE COUNT          240 a          300 c          355 g          237 t
ORIGIN      
        1 tgatgtagcg caggcggtcg gtaatggtat cgatgttcga gcggcggttg tccaaggcat
       61 cggggtagac cacgaagttc tcaaaagcca ggcactggga gtagtcgagt ttctcttggt
      121 aatccgggtc gggcgggtca ccgtcgaatt cggcggcgac gatatcgtcc ttgtcggcgg
      181 ccagggagat gtcgatggtc tccggcgcgg agcacattgc aaatacgaaa ccgccggagg
      241 cgacgtaatc cttgattgtc cgggcgaccg cttttttctc ttcggaaact ttgcggaagc
      301 ccaaggcatg ggctaacgct tcgttggtcg ccacttcttc gcggtaccag agcgcattcc
      361 ggtaagcgcc gtagaacttc ccgtactggc cggtgaagtc ctcgtggtgc aggtggagcc
      421 agtcgtactt ggccaggtcg ccgcgtagaa cttcggtgtc ccacaaggtg gtgtagggaa
      481 tttcggcata gtccaaagcc aggcgtaccg cgtcgtccca gggctcgcgg ttggcaggca
      541 cgtagacggc caattttggc gctttttcca ggagcaccac gtccatattg ccctgttcta
      601 tcgtctggta aatctggccc gctccggcag cgtcgatggg tgtaaaagtg actccccgca
      661 ggcggcagag gtctgccaac gcctgccagc cgtcggtcaa aaacgagccg ccgcggtagt
      721 tcaatagcca ctcgacgttg tgagcgttct gcaagcacca gaaggcaatg ccgtaggcct
      781 tcaggtggtc ggcctgggag agatccatcg ggatcaagag cttctgggct gacgcgggtt
      841 cgcacaggga gaggaaaaca acgagagcca gaagtagggg cactacgaat tttaatggcc
      901 ggacgcgcat cttttggtta tacggcagaa aatacccttt ctgaaagaat tttacgggaa
      961 ttgcactggg aaattcaaga gaaaagcgac gaaatggccc ggggcaggtg atcgagaata
     1021 tcccccgcaa tgagactgta ctcgtttttt gcatcacggc tcagatcgcc ggctaagccg
     1081 tggaggaaca caccggcaca ggccgcttga accggtttgg ctccctgcgc cc
//
LOCUS       DBNGZA010000352         2751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1869993, whole genome shotgun sequence.
ACCESSION   DBNGZA010000352 DBNGZA010000000
VERSION     DBNGZA010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1869993"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="AC16FU_07790"
     CDS             complement(<1..2160)
                     /locus_tag="AC16FU_07790"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR2994306.1"
                     /translation="MTLENDALTYHRTGRPGKLEVVATKPFLTQRDLSLAYTPGVAEP
                     CRRIVADPQEVFDYTAKGNLVAVITNGTAVLGLGNIGPLAGKPVMEGKAVLFKRFADI
                     DVFDLEIAATDPDEFVRTVQLLEPTFGGINLEDVRAPECFRVEQELARTLSIPVFHDD
                     QHGTAIITSAGLLNALELVDKEIEKVKVVVVGAGAAGIACARLFISLGLKQEHLILVD
                     SKGVVYRGREVDMNPYKESLAADTKARTLADALVGADVLVGVSAPNLVTPQMLARMAP
                     KPIIFACANPDPEIPYPDAKKARPDAIVATGRSDFPNQVNNVLGFPFVFRGALDVRAT
                     AINEAMKKAAVRALADLAKQDVPDIVAKAYGVESFRFGPEYILPKPFDPRVLLWEAPA
                     VAQAAMESGVARKKIDLEVYRNALEARLGKSRELMRIVANKAKRAPKRVVFPEGTHPK
                     ILRASHHLVDEGIARPILLGDRAQIEAGANDLGLPLVGIEIIDPHQCPQLDSYVEELF
                     SLRQRKGLTRAEARQLLVKNPNYFGAMMVHTGDADALISGITQHYPDTIRPALQVIGM
                     RPDVNRVAGLYMIITKSQAVGQRILLFADTTVNIQPTAEDLAEIALLTAEIARRFDLV
                     PKVAFLSYSNFGSVRNPQVLALQEAVRLVKKRAPDLIVDGEMQVDSAVDPEVAAEHFP
                     FSAIQGDANVLIFPELFSGNIAYKMMQRLGGAEVIGPI"
     gene            2388..>2751
                     /locus_tag="AC16FU_07795"
     CDS             2388..>2751
                     /locus_tag="AC16FU_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2994307.1"
                     /translation="MIYSRGCEYAIRALAYMANQSGERFISAKEIGKKEGVPTFFLAK
                     TLQKLAQAGVLRSFKGPTGGFGLARSPKQITLYDIVEAIDGTGDLNRCAAGLVACSDA
                     APCPMHSQFKQLREHVQRY"
BASE COUNT          548 a          792 c          851 g          560 t
ORIGIN      
        1 aatcgggccg atcacctcgg cgccgcctaa gcgctgcatc attttgtagg cgatgttgcc
       61 ggagaaaagt tccgggaaaa ttaagacgtt ggcgtcgccc tgaatggcgg agaaggggaa
      121 gtgctcagcg gctacttccg gatcgactgc ggaatcgacc tgcatttcgc cgtccacgat
      181 taaatccggc gcacgctttt tgaccagccg caccgcttcc tgaagcgcca gaacctgcgg
      241 gttgcgcacg ctgccgaaat tcgagtacga gaggaaggcc accttcggga ctaagtcgaa
      301 gcgccgggcg atttccgctg taagaagggc aatttcggcc aagtcttccg ctgtgggctg
      361 gatgttgacc gtggtgtcgg caaagagcag gatccgctgt cccaccgcct gcgacttggt
      421 gataatcata tacaacccgg ccacccggtt gacgtcgggg cgcattccaa taacctgcag
      481 ggcggggcgg atggtgtccg gatagtgctg ggtgatgccg gaaatcaggg cgtcggcgtc
      541 gccggtgtgc accatcatcg cgccgaagta gttgggattt ttcaccaaga gctgacgcgc
      601 ttcggcacgc gtcaatcctt tgcgctggcg cagggagaaa agttcctcca cgtaggagtc
      661 gagctggggg cattggtgcg ggtcgatgat ctcaattccg acgaggggaa gtcccaggtc
      721 gttcgcgccg gcttcgatct gcgcccggtc gcccagaaga atcggcctgg cgatgccttc
      781 gtccaccaga tggtggctgg cgcgcagaat cttgggatgg gtgccttccg gaaagacgac
      841 ccgcttgggg gcgcgcttag ctttattcgc cacaatccgc atcagttcgc gggatttccc
      901 caggcgggct tccaaagcat tacggtaaac ctcaaggtcg atctttttgc gggcaacgcc
      961 ggattccatc gccgcctgcg ctacggcggg tgcctcccag agcagtaccc gcgggtcgaa
     1021 cggcttgggc aagatatact ccgggccaaa gcggaacgac tccacaccgt aggctttggc
     1081 cacgatgtcg ggcacatcct gcttggccaa gtcggctaag gcgcggacgg cggctttttt
     1141 catcgcttca ttgatggcgg tggcgcgcac gtccaaagct ccccggaaga cgaaggggaa
     1201 tccgaggacg ttattcacct gattgggaaa atcagacctg ccggtggcga cgatggcatc
     1261 gggccgggcc tttttcgcat ccgggtaagg gatttcggga tcggggttgg cgcaggcgaa
     1321 gatgatcggt ttgggggcca tcctcgccag catctgggga gtcacgaggt tgggggccga
     1381 aacgccgacc aaaacgtcgg cgcccaccag cgcgtcggcc agcgtgcggg ccttggtgtc
     1441 ggcggctaag ctttctttgt agggattcat gtccacttcc ctgccgcggt agaccacgcc
     1501 cttactatcg accaggatca agtgctcttg cttgaggccc agagaaataa agagacgggc
     1561 gcaggcgatc cccgccgcac cggcgccgac gaccacaact ttcactttct caatttcctt
     1621 atccaccaat tccagcgcgt tcagcaaacc ggcggaggtg atgatggcgg tgccgtgctg
     1681 gtcgtcatgg aaaaccggga ttgacagtgt ccgggccagt tcctgctcga cccggaagca
     1741 ttccggggca cggacgtcct ctaagttaat gccgccgaaa gtcggctcca gaagctgcac
     1801 cgtgcggaca aactcatccg gatcggtcgc cgcgatttcg aggtcgaaga cgtcgatgtc
     1861 ggcgaagcgc ttgaagagca ctgctttgcc ttccatcacc ggcttgccgg ccagggggcc
     1921 gatattgccc agacccagga cggcggtgcc gttggtaatc acggcgacca aattcccctt
     1981 cgcggtataa tcaaacactt cctgcgggtc ggccacaatc cggcggcagg gctcggccac
     2041 gccgggggtg taggcgaggg agaggtcgcg ctgggtcaaa aacggcttgg tggcgacgac
     2101 ctctaatttt ccgggccggc cggtgcggtg gtaggtcagg gcgtcatttt cgagtgtcat
     2161 cgtttcttac ttcctcacaa aggtgtgtgt gggagcatcg cgtgcgcggg aataatcatg
     2221 cccggcagta ttataattgc gccaaaacca tcttggcaat cgttggtgaa aagcttttca
     2281 ggtttcccgg gcacttgaca tcaccacatc cttatacgta taatatatta tgataaatgc
     2341 ctccggtaat caaaattttg ggccgtccac ctggaacaag gagacttatg atctactcac
     2401 gcggatgtga gtacgccatt cgggcactgg cctacatggc caatcaatcg ggagagcggt
     2461 tcatttccgc caaagaaatc ggcaaaaagg agggtgtgcc gactttcttt ttggccaaga
     2521 ccttgcaaaa gttagcccag gccggagtgc tccggtcgtt caaagggccc accggcggct
     2581 tcgggctcgc ccgatcgccc aagcagatta ccctctatga catcgtcgag gccatcgacg
     2641 gcaccggcga cctcaaccgc tgcgccgccg ggctagtggc ctgttccgac gcggcaccct
     2701 gtccgatgca tagccagttc aagcagttgc gggagcacgt ccagcgctac c
//
LOCUS       DBNGZA010000353         1871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_171532, whole genome shotgun sequence.
ACCESSION   DBNGZA010000353 DBNGZA010000000
VERSION     DBNGZA010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_171532"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="AC16FU_07800"
     CDS             complement(<1..618)
                     /locus_tag="AC16FU_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994308.1"
                     /translation="MRAHALKKLLLLLVLVTGLWPLAPGPCLADFYGTYGNGTAAGDT
                     VRIVFGTQDTTFLNAADCDSFFILRFFRGTLLDSTADDAAGVAKLRTGYYVARFRAAN
                     ASGAAGHYDVRILAFKQGKTGIKTGTYQVVDGGILDEGDSSKLARSVWDNDLVAQANR
                     TVANVGSGTGAYEVKLFTKRSDSTVIAGTQVQVLNQAQTATVGLLT"
     gene            complement(702..1406)
                     /locus_tag="AC16FU_07805"
     CDS             complement(702..1406)
                     /locus_tag="AC16FU_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994309.1"
                     /translation="MRKAQNEFGVDRLVPEPVNECRGEPSGSPGNLHLPTAATLLWLL
                     WLSPHAHADTQTLRPTGTGFYTGACVLVGPANWQDVDEFTPDENSTYVYAEGVIDLCG
                     SPVQDTYQAQDFSGSGTLDSVVVTIRCAKSAAAGTNTAQTLIRVNSKDSLGTSNTVTT
                     TFADYRYQYAQKPGGGSWTVADVNALEIGAKLIASATGGNTKCTQVFATVYYAPQASA
                     RPKGSLIADEETGVVK"
     gene            complement(1387..1710)
                     /locus_tag="AC16FU_07810"
     CDS             complement(1387..1710)
                     /locus_tag="AC16FU_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994310.1"
                     /translation="MAGVNSVTRRKKAGRKNGASAPSRKRRKLSEEDRKLLTEFLEKS
                     LEQLRSGHISIKAADLLRALAMKSEAAANGDEKQLFQMIDEFKKETGEEPARPETGTD
                     AESAK"
BASE COUNT          342 a          542 c          571 g          416 t
ORIGIN      
        1 ggtgagcaga ccgacggtgg ccgtttgcgc ttgattcagc acctgcacct gcgttccggc
       61 aattaccgtt gagtccgacc tcttagtgaa gagtttgacc tcataggcgc ccgtcccgga
      121 gccgacgttc gcaacagttc tattcgcctg cgccaccaga tcattatccc agaccgagcg
      181 ggcgagtttg ctcgaatcgc cttcgtcgag aataccgccg tctaccacct gataggttcc
      241 cgtcttgatg ccggttttcc cttgcttgaa ggccaaaatc cgcacgtcgt aatgccccgc
      301 agcgccggac gcattagccg ctctgaatct tgccacgtag tagccggttc gcagtttggc
      361 aacaccggca gcgtcgtcgg cggtgctgtc caaaagcgtt ccccggaaga aacggaggat
      421 gaagaagctg tcgcagtcgg ccgcgttgag aaacgtggtg tcctgcgtgc cgaagacgat
      481 gcggaccgta tcaccggcgg cggtgccgtt gccgtacgtg ccgtagaaat ccgcaaggca
      541 ggggccagga gccaggggcc agaggccagt gaccagcacg agcaaaagca agagtttttt
      601 aagagcgtga gcgcgcattg acccctcccc agccctcccc tctaagggga gggagagtgg
      661 ggggccagag gttttctgga aggaagcggc ggacagtttt ctcatttcac tactccagtc
      721 tcttcgtcgg cgatcaatga tcctttcggg cgggcgctcg cctgcggcgc atagtaaacc
      781 gtcgcaaaaa cctgcgtgca tttggtgttg ccgccagttg ccgaggcgat caatttcgct
      841 ccaatctcca gcgcattcac gtcggccacc gtccagctgc cgccgccggg tttctgagcg
      901 tattgatagc gatagtcggc gaaggttgtg gtgacggtgt tggaggtgcc gagtgaatct
      961 ttgctgttga cgcgaatcaa cgtctgcgct gtgttcgtcc cggcggccgc gctcttcgcg
     1021 cagcggatgg tgacgacgac tgagtcaagt gtgcctgaac cgctgaagtc ttgcgcctga
     1081 taggtatcct gcaccggact gccgcagaga tcgatcactc cttccgcgta gacgtaggtc
     1141 gaattttcat ccggcgtgaa ttcgtccaca tcctgccagt tggccggtcc gaccaagacg
     1201 caagccccgg tgtagaaacc ggtgccggtg gggcggaggg tttgcgtgtc ggcatgggca
     1261 tgaggggaga gccaaagaag ccataggagt gtagccgcag tggggaggtg aagatttccg
     1321 ggcgaacctg aaggttcgcc cctacattca ttgaccggct ctgggacgag ccggtctacc
     1381 ccgaattcat tttgcgcttt ccgcatcggt tccagtctcc ggtcgggccg gttcctcccc
     1441 ggtttccttt ttgaactcgt cgatcatctg gaagagttgt ttttcatcgc cgttggcagc
     1501 cgcctccgat ttcatcgcca gcgcgcgcag gaggtccgcg gccttaatgg agatgtggcc
     1561 gcttcggagt tgctccagac ttttttctaa gaattcggtc aggagttttc gatcttcttc
     1621 cgaaagtttg cggcgcttcc gtgaaggcgc cgaagctcca tttttccggc cggctttttt
     1681 tcttcgggtt accgagttaa cgcctgccat tccctatccg agctccagaa tgagcaaatc
     1741 caccaccgcc gggtcggcgc cggcatcggt gcgggccagg cggaaggaga cattggattc
     1801 cggcccggcc acgatcacct gcgcattcga cttgggcagg acgatgacct tagggctgga
     1861 aagtgtcgcc g
//
LOCUS       DBNGZA010000354         5115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_703793, whole genome shotgun sequence.
ACCESSION   DBNGZA010000354 DBNGZA010000000
VERSION     DBNGZA010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_703793"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="eno"
                     /locus_tag="AC16FU_07815"
     CDS             complement(<1..1019)
                     /gene="eno"
                     /locus_tag="AC16FU_07815"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR2994311.1"
                     /translation="MKEKVQTIKAREILDSRGKPTIEVDCILTNGMVGRAAVPSGAST
                     GAHEALELRDGDPRRYLGKGVLKAVENVNRTIGPALIKKGIDLFVQEAMDQFLIDLDG
                     TPEKKKLGANAVLGVSLAWAKAAALAQGVPLYRYLGGDEAHVLPVPLLNVINGGRHAD
                     NNLDFQEFMLAPVGAPSFGEALRMGAEIFHTLQKIAKQKGHSIAVGDEGGIAPNLKCE
                     EEALELLSESARQAGYIPGEVLYFALDPAASEFYDSRTGQYVLKGAGKTYTSSQLLDR
                     YERLVDQFPIFSIEDGLAEDDWDGWKELTGRLGEKIQLVGDDLFVTNPSRLSRGIKEK
                     AANAI"
     gene            complement(1028..1834)
                     /locus_tag="AC16FU_07820"
     CDS             complement(1028..1834)
                     /locus_tag="AC16FU_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR2994312.1"
                     /translation="MSVERLVWSGLAALVIVSVSLRLRSLSFPGAVAAWVAGSVTYVF
                     GGWAAVVPLLFFFVSGSLLSRWPERPSKAISLEKPGPRDGWQVLANGGLATVFIVLAF
                     LFPSRNFHLAFLAAYAEAGADTWATEIGLLSRIAPRSIISRKMVSPGTSGGVTALGLL
                     AGFVGALFTAACGFWIQQKEWGWTLGQVALAGTSGFAGSLFDSVLGATVQQKFRCPDC
                     GQMTERRECRCGGKTVFASGIRGLNNDWVNFLSTFFSAGFALLLVGLFAY"
     gene            complement(1844..4042)
                     /locus_tag="AC16FU_07825"
     CDS             complement(1844..4042)
                     /locus_tag="AC16FU_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994313.1"
                     /translation="MWPGSWSDFTVALLERKGYLVFGGIALYSAAVFFYYRRTLPPLN
                     VKILRLLGGVRWLAVAAVFAGLSEPLVSYEKVSRLPPTVAVLVDASRSMAFREGEKTR
                     REQALELAGQPWIDELKRKVVVVSYDFADSLLPFPPAAMGRGSAAQAASGASTNIGRA
                     LRSLGQKYREQNLSGVILLSDGANNAGPDPVGAAWQSGFPIYPCAVGADVVPADVSVD
                     QVRAPEVAYAKEKVPVTISVSSAGFEGKRIPVEIRFDKKVVTRTLLTLAGQGKKQEVS
                     LELAADSAGLFTYRVSVPLQAGERLRVNNERQFVLKVLPNKIRVGILAAFPSWEYRFL
                     VQALEQSGGFEVASYFTNAPPVNASGFRWSEPALAQTDLLLLLDPPGAFLGGKEARLR
                     KFVEREGKGLALFFGPQLVSSWRAVDQATFPFVSGLIAEQSGTFGLELTVDGKRNGLT
                     RLVANDEENEKLWTTLPPLLQVVAVQKSWLGGVPLVTATDQRSGQTIPVVTVGPWGQG
                     KVFLVTGSPLWRWSFFAADLNRYPESYALFWKNCLQWLSTKDDLNRVRVSTDRLVYKA
                     GEEVLITGSVFDERYQPLAGAQVKLQAKNLDSNATTATAELFEEESGQYQTALRALAP
                     GRYQLILAAAKEGKQVGEAAARFTVEQFGLEDENLSVDRGLLEELARASGGKFYTPAT
                     LAQIVNDLQPQAESMAQKREVEVWNSPLLFLAIVLLLGTEWALRKRYQLL"
     gene            complement(4142..>5115)
                     /locus_tag="AC16FU_07830"
     CDS             complement(4142..>5115)
                     /locus_tag="AC16FU_07830"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR2994314.1"
                     /translation="PIQSDLEAFERLLERSLVSDADLITEINRHLLARRGKRLRPAIV
                     FLAARLCDVGRGTAAAPFSLRAGYAALAIELIHTATLLHDDVVDESPTRRGQATVNAK
                     WKNLIAVLMGDYYFSKAFTLLVKAETEGLMGAVSTATERVSVGQLLEIQESRNFGVTE
                     AAYFRIIQEKTASLFGAAAEAGALVAGAPLAAQLALREYGEKLGNAFQIADDGLDLVG
                     KVEKTGKDLGTDLREGKVTLPLIFALAQAADGNRERIVQLLENGMVDGDFSEVVSFIR
                     TQGGLEYASRKARTFGSEAQALLASFPDSAYRQALSLLARFAVERER"
BASE COUNT         1082 a         1638 c         1434 g          961 t
ORIGIN      
        1 aagatcgcgt tcgccgcttt ctcctttatc ccgcgtgaca aacgggaggg attcgtgaca
       61 aagaggtcat cgccgaccaa ttgtattttc tctcccagac gcccggtcaa ttctttccag
      121 ccgtcccagt cgtcttccgc cagcccgtcc tcaattgaga aaatggggaa ttgatcgacc
      181 agccgctcgt accggtcgag caactgagaa gaagtatacg tcttgcccgc acctttcagg
      241 acgtactgac ccgtacggga atcataaaat tccgatgcgg caggatcgag cgcaaagtag
      301 agaacttctc cggggatgta ccctgcctgt cgcgcgcttt cggagagtaa ttctaaagct
      361 tcctcttcgc atttgagatt cggcgcgatc ccgccctcgt ccccgacggc gatgctgtgt
      421 cctttttgct tggcaatttt ctgaagcgtg tggaagattt ccgcgcccat ccgcagggct
      481 tcgccgaacg aaggcgcgcc caccggagcg agcatgaact cctggaaatc gagattgttg
      541 tcggcgtgcc gcccgccgtt aatcacgttc aaaagcggca ccggcagcac gtgcgcctca
      601 tcgccgccga gataacggta gagaggaacg ccctgcgcca aagccgccgc cttcgcccag
      661 gcgagcgaga cgcctaaaac cgcattcgct cctaattttt tcttctccgg cgtgccatcg
      721 aggtcgatta agaattgatc catcgcctct tgaacgaaaa ggtcaatgcc tttttttatc
      781 agagcgggac cgatggtacg attgacgttc tcgaccgctt tcaaaacgcc ttttcccaga
      841 tagcgccggg ggtcgccgtc gcgcagttcc agcgcttcgt gagcgccggt cgaggcgccg
      901 gacggcaccg ccgcgcggcc caccatgccg ttggtaagga tacaatcgac ttcgatggtg
      961 ggtttccccc gcgaatccag aatctcgcgg gctttgatgg tttggacttt ttccttcatc
     1021 ggcgcgttca atatgcaaaa agccctacca aaagcaatgc gaaaccggcg gaaaagaaag
     1081 tggaaaggaa gttgacccag tcgttattca gcccgcgaat cccggaggca aataccgtct
     1141 tcccgccgca acggcactcc ctgcgctccg tcatttgccc gcagtcaggg cagcggaatt
     1201 tctgctggac ggtggcaccc agaactgaat cgaaaagact tcccgcaaag ccgctggttc
     1261 cggccagtgc cacctgcccg agtgtccatc cccactcttt ctgctgaatc cagaagccac
     1321 aagcagcggt aaaaagcgct cccacaaaac cggccaacaa tcccagcgcg gtaacaccgc
     1381 ctgaggttcc cggagatacc atcttgcgcg aaataattga gcggggtgcg atccgagaca
     1441 ggaggccgat ttcggtcgcc caggtatcgg cgccggcttc ggcgtaagcc gccaggaacg
     1501 ccaaatgaaa attgcgagag ggaaaaagaa acgcgagcac gatgaagacg gtcgctaggc
     1561 cgccgttggc caaaacctgc cagccgtcgc gcggtcccgg cttttccagt gagattgctt
     1621 tcgaagggcg ctccggccag cgggaaagca ggctgccgga aacgaagaaa aaaaggagcg
     1681 gcaccacagc cgcccacccc ccgaaaacgt aggtgacaga acccgcaacc caggcggcca
     1741 ctgctcccgg gaacgaaagc gagcggagac ggagtgaaac cgagacgatg accagcgcgg
     1801 ccagcccgct ccagaccaag cgctcgacgc tcaccggctc agactataac agttgatacc
     1861 gtttgcgcag cgcccattca gttcccagca agaggacgat ggccaggaat aacagcggag
     1921 aattccagac ctcgacttcc cttttctgcg ccatcgactc ggcctgcggc tggaggtcgt
     1981 tcacgatctg cgccaaagtc gccggtgtgt agaacttccc gccgctggcg cgggcgagct
     2041 cttccaaaag cccccggtcg accgagaggt tttcatcttc caatccgaac tgctccacgg
     2101 taaagcgcgc cgcagcctcc cccacctgct tcccctcctt ggcagccgca aggatgagct
     2161 gataccgtcc cggagccagc gcccgcagcg ccgtctgata ctggccggac tcttcttcaa
     2221 aaagctctgc ggtcgccgtc gtggcgttgc tgtcaaggtt tttcgcctgg agtttgacct
     2281 gtgccccggc cagaggttga taccgctcgt cgaagaccga accggttatc aggacctcct
     2341 cacccgcttt gtaaaccaat cggtcggtgg agacgcgcac ccggttcagg tcgtctttgg
     2401 tcgaaagcca ctgcaggcaa ttcttccaaa acagagcgta actctccggg taccggttaa
     2461 ggtcggcggc aaaaaaggac cagcgccaga gaggtgaccc ggtaaccaaa aacaccttgc
     2521 cctgtcccca cggtcccacg gtgacgaccg gaatggtttg gccggaacgc tggtcggtcg
     2581 ctgtcaccag cggcactcct cccagccagc tcttctgcac cgccaccacc tgcaaaagcg
     2641 gcggcagtgt tgtccagagt ttctcgtttt cctcatcgtt ggcgaccaaa cgcgtcagac
     2701 cgttacgctt cccgtcgacg gtaagttcca agccaaaggt tccggattgc tccgctatca
     2761 agccggagac gaagggaaac gtcgcttgat cgaccgctct ccaggaagaa acgagctggg
     2821 ggccaaagaa gagtgccagc cctttgcctt cccgttcgac gaacttcctc aaacgcgctt
     2881 ctttcccgcc cagaaaagcc cccggcggat cgagcaagag caggagatcg gtctgggcca
     2941 gtgccggctc gctccagcgg aaaccggagg cgttgaccgg cggcgcgttc gtgaaatacg
     3001 aggcgacttc aaatccgccg ctctgctcca gcgcctgcac caggaaccgg tattcccacg
     3061 aaggaaaagc ggcgaggatg ccgacccgaa ttttgttcgg cagcactttg agaacgaact
     3121 gacgctcgtt attcactctc aaccgctcgc ccgcctggag cggaacgctc acccggtagg
     3181 tgaagagtcc ggccgaatcg gcggcgagct ccagggaaac ttcctgcttc ttcccctgtc
     3241 cggctaaggt gagcagtgtt cgggtaacga cttttttatc aaagcgaatc tcgaccggta
     3301 tgcgctttcc ttcgaacccg gcactggaga ccgatatggt gaccggcact ttctctttcg
     3361 cgtaggcaac ctccggcgcc cgaacttggt ccacgctgac atccgccggt accacgtcgg
     3421 ctcccaccgc gcaggggtaa atcggaaaac cgctctgcca cgccgctccc accgggtccg
     3481 gccccgcatt attggccccg tcggagagca agatgactcc ggagagattt tgttcccggt
     3541 acttctgacc cagtgagcgc agagctctcc cgatgttggt gctggcgccg ctggcggcct
     3601 gcgcggccga accacgcccc atagcagcgg gcgggaaagg caaaagcgag tcggcgaaat
     3661 cataagagac gactaccact tttcttttga gttcatcgat ccagggctgc ccggcgagtt
     3721 ccagcgcctg ctcccggcgg gtcttttccc cttcgcggaa ggccatgctg cgtgaggcgt
     3781 cgacgaggac ggccacagtc ggtggcaggc gggaaacttt ctcgtagctg accaggggct
     3841 cgctcaaccc ggcaaagacc gccgccaccg ccagccagcg cactcccccc aataaccgga
     3901 gaattttgac atttaaaggc ggcagcgtgc gccggtagta aaagaacacc gccgccgagt
     3961 agagggctat cccgccaaaa acgaggtagc ccttccgttc caaaagtgcc accgtaaaat
     4021 cagaccagct acccggccac atcctcttcc tgtagtacga tagattcttt ccggcaacgc
     4081 tgcatcgtgg atacaaaaaa accaccgcca gggcagtggc tggaaccaga acagtttgcg
     4141 cttaacgctc cctctcgacc gcaaagcgcg ccaaaaggga gagcgcctgg cggtacgccg
     4201 aatccgggaa agaggccaaa agcgcctggg cctcgctccc gaaggtgcgc gccttccgcg
     4261 aagcgtactc cagccctccc tgggttcgga tgaaactcac tacctcggag aagtccccgt
     4321 caaccatccc gttttctaag agctgcacaa tgcgctcccg gttgccgtcc gcggcctgag
     4381 ccagagcgaa aatgagcggg agggtcacct ttccttcccg caggtcggtt cccagatcct
     4441 tgccggtttt ttccaccttg ccgaccagat ccaggccgtc gtcggcgatc tgaaaagcgt
     4501 ttcccaattt ttctccatac tcccggagcg ccagttgcgc tgccagcggt gcgccagcca
     4561 cgagagcccc cgcttcggcg gcggcgccga agagtgaggc ggtcttctcc tgaatgatcc
     4621 ggaagtaggc ggcttcggtc acgccgaaat tccggctctc ctgaatttcc aaaagctgcc
     4681 ccaccgacac ccgctcggtg gcggtggaga ccgcgcccat gagaccttcg gtttctgctt
     4741 tcactaaaag ggtgaaggct ttggagaagt agtagtcgcc catgagcact gcgatgagat
     4801 ttttccactt ggcgttcacc gtggcctggc cccgtcgggt cggagattcg tccaccacgt
     4861 cgtcgtgcaa aagcgtggca gtgtgtatca gttcaatggc caaggccgcg taccccgcgc
     4921 gcagggaaaa gggcgctgcc gccgtcccgc gcccaacgtc gcagagccgc gccgccagaa
     4981 aaacgatcgc cggccgcagc ctttttcccc ggcgtgccag aaggtgccgg ttgatttcgg
     5041 tgatcaggtc ggcgtcactt acgagcgacc gctccaggag gcgttcaaac gcttccagat
     5101 cagactgaat cggag
//
LOCUS       DBNGZA010000355         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_703928, whole genome shotgun sequence.
ACCESSION   DBNGZA010000355 DBNGZA010000000
VERSION     DBNGZA010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_703928"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="AC16FU_07835"
     CDS             complement(<1..1386)
                     /locus_tag="AC16FU_07835"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR2994315.1"
                     /translation="MAIERETEILDALRKVHDPDLGRDIVSLGFVKNLRVADGNVAFT
                     VELTTPACPVRDALKEEAQRVVLALPGFKKVEVTMTAQVRTTPSEKKEKMVPAVKNIV
                     PVASGKGGVGKSTVSANLAVALAQLGARVGLMDADIYGPSIPTIMGVKQPPTVLSGNR
                     LIPTVEHGVKIISIGFFIRPGDAVIWRGPMLHKTVEQFLANVEWGELDYLIVDLPPGT
                     GDVQLSLCQTIPLTGAAVVATPQDVALQVAEKAIIMFGKLNTPVLGLIENMSGFVCPH
                     CGAREDIFGAGGARAYSLAHDIPFLGEIPLATQIRETSDSGLPIVIAAPDSPAARAFA
                     QAAQNLAAQVSIRNLGGVPPKAGEDRPTPAEISQPKREQVSILWKDGHASLYPARDLR
                     LACLCALCVDEVSRKPLLREEKVPQNVHPNIIHPVGRYALRFEWSDGHSTGIYTFDYL
                     RSLCPCEKCRAK"
     gene            1766..>2024
                     /locus_tag="AC16FU_07840"
     CDS             1766..>2024
                     /locus_tag="AC16FU_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994316.1"
                     /translation="MRNGERESVSGRESLLGLLLKDRERFFSEVADNVGIKEKLKFLL
                     TTLIGLSAFYGATVGLYQGGWQILSAALKIPFLFLVTLLISF"
BASE COUNT          439 a          556 c          570 g          459 t
ORIGIN      
        1 ctttgcccgg cacttttcgc aaggacagag cgagcgcagg tagtcgaagg tgtaaatgcc
       61 ggtggaatgc ccgtcgctcc actcaaaacg cagggcgtag cgccccaccg ggtgaatgat
      121 gttcggatga acgttttggg gaactttttc ttcccgcaaa agaggtttgc ggctcacttc
      181 atccacgcac agggcgcaga gacaggccag gcgcaggtcg cgggccgggt acaagctcgc
      241 gtgtccatct ttccagagga tcgagacttg ctctcgtttc ggctgggaga tttcagccgg
      301 agttggacgg tcttctcccg cctttggcgg gacaccaccc agattccgga tacttacctg
      361 cgcggccaag ttctgggccg cctgagcgaa ggccctggcg gcaggcgagt ctggggcggc
      421 gataactatc ggcagtccgg agtcggaagt ctctctgatc tgagtggcta aaggaatttc
      481 gcccaggaac ggaatgtcat gcgctaaact gtacgcccgc gccccgccgg cgccgaaaat
      541 atcctcccgc gccccacagt gcgggcagac aaagccgctc atattttcaa tcaaacccag
      601 aaccggcgtg ttcagtttgc cgaacatgat gatggccttt tccgctacct gcagggccac
      661 gtcctgcggc gtggccacca ccgccgcgcc ggtcaggggt attgtctggc agagcgagag
      721 ctggacgtcg cccgtgccag gcgggagatc aacgatcagg tagtcgagtt caccccattc
      781 aacgttggca aggaactgtt cgaccgtctt gtgcagcata ggaccgcgcc agatgacggc
      841 atcgccgggc cggatgaaga agccgatgga gatgatcttg acgccgtgct caaccgttgg
      901 aatgaggcgg ttgccgctca aaactgtcgg cggctgtttc actcccatga tggttggaat
      961 gctggggccg tagatgtcgg catccatcaa ccccacccgg gcgcccagtt gcgccagggc
     1021 caccgccagg ttggcgctga cggtggactt tcccacaccg cctttgcccg aggcgaccgg
     1081 cacaatgttt ttcaccgccg gaaccatttt ctctttcttt tcggaggggg tggtccgcac
     1141 ctgggcagtc atcgtcactt ccactttttt gaatcccggc agggccagca ccacccgttg
     1201 agcttcttct ttcaacgcgt cgcgcaccgg gcaggcgggg gtagtcagtt caacggtaaa
     1261 ggccacgttg ccgtcggcca cccggaggtt tttgacaaag cccagactga cgatgtcgcg
     1321 gcccaggtcg ggatcgtgaa ccttccgcag ggcgtctaag atttcagttt cgcgctcaat
     1381 ggccatggga cgctccggtc atttcagcgg ttgccgactc aacggttttg ctcttgagca
     1441 ccttacaact cctttgagtg gggaaaacgg ttctttcccc tccctcaaat gtaatataac
     1501 ctaaatagaa atgagtatga actctatccg gagttcggtg ggtcctacag aaagccgcaa
     1561 gtaagcaacg ggaggattcg agttattcct gcgagaaaaa atggtttgtc tccccttctc
     1621 aattacccgg tctcggattt caagacttaa tattgtagtc gactattgtg aatcaaacgt
     1681 agtcaaataa taatatacaa taaaaaacag ttgaatatcg tattattcac agaaaacgta
     1741 attaaccaga aagcgaggcg gatttatgag aaatggagaa cgagagtcgg taagcggcag
     1801 ggaatcactt ttggggttgt tattgaaaga tcgggagagg tttttcagcg aggtcgccga
     1861 taacgtcggg ataaaagaga aactgaaatt tcttctgacc actctcatcg gtctttccgc
     1921 gttttacggc gcaaccgtgg gactgtacca gggcggctgg caaattctct cggcggcgct
     1981 aaaaattcct ttccttttcc tcgtcaccct gcttatctcc tttc
//
LOCUS       DBNGZA010000356         1113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_171864, whole genome shotgun sequence.
ACCESSION   DBNGZA010000356 DBNGZA010000000
VERSION     DBNGZA010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_171864"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1113
                     /locus_tag="AC16FU_07845"
     CDS             <1..>1113
                     /locus_tag="AC16FU_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR2994317.1"
                     /translation="TFDVSLTFPAHFIVAATGLLQNRNEVLPESLCQKLRIENFKDKP
                     WGSPASSVIPKEEGKNKTWRFRAENVHDFAFIADPTFRIGEAEWEGIKIYAYCQEKHA
                     SGWQDAAALGARAIQYFSETFGRYRYPQMIIGDSYDGMEFPMLVMCGGRSPSYRLVVY
                     HEIGHNWYMGQVGSNQVDRAFLDEGFTTFMEINCIETFPARKGNINFFDKWYEKKYYP
                     QDEDRAARGYRPYYQWSKSGYAQRMIISADKAPENNVYRVSSYYKTVALLFNLQYVLG
                     DSLFWQGMRTYFERWHFKHPYEDDFQTVMEEVSGRELDWFFDEWMQTTKTIDYAVQRP
                     RGKWVEADSGKVYRMQLRLSRKGESIMPLDLRVTLQN"
BASE COUNT          265 a          304 c          314 g          230 t
ORIGIN      
        1 acgttcgacg tgtcgctgac ctttcccgcc cacttcatcg tcgcggccac cggcctcttg
       61 caaaatcgaa acgaagtgct gccggaatcg ctctgccaaa aactcagaat agagaatttc
      121 aaagacaagc cctggggctc accggcgtca agcgtcatcc cgaaagaaga agggaaaaac
      181 aaaacctggc gtttccgcgc cgagaatgtc cacgattttg cgtttattgc cgatcccact
      241 tttcgcattg gcgaggccga atgggaggga attaaaattt acgcctattg tcaggagaag
      301 cacgcctccg gctggcagga tgcggcggca ttgggtgcgc gggcgattca gtatttctcc
      361 gaaaccttcg gccgctaccg ctacccgcaa atgatcatcg gcgattcgta cgatgggatg
      421 gaattcccga tgctcgttat gtgcgggggg cgctcgccct cgtaccggct ggtggtctac
      481 cacgaaatcg gccacaactg gtacatgggg caggtgggat cgaaccaagt ggaccgggca
      541 tttttggacg aggggtttac gacctttatg gagatcaact gcatcgaaac attccccgcg
      601 cggaaaggga atatcaattt ctttgacaag tggtatgaga agaaatacta cccgcaggat
      661 gaagaccgcg ccgcccgcgg ctaccggcct tactaccagt ggagcaagtc cggctacgcc
      721 cagcggatga tcatctccgc cgacaaagcg cccgagaata acgtctaccg cgtctcatcg
      781 tactacaaga ccgtggccct gctcttcaac ctccagtacg ttttaggcga ctcgctcttc
      841 tggcagggga tgcggaccta tttcgaacgg tggcatttca agcacccgta cgaggacgat
      901 tttcagacgg tgatggaaga agtttccggc cgggaacttg actggttttt cgacgagtgg
      961 atgcagacga cgaaaaccat cgattacgcg gtgcagcgac cgagagggaa gtgggtcgaa
     1021 gccgacagcg gcaaggtcta tcgtatgcaa ctgcgcttat cgcgcaaagg cgagtcgata
     1081 atgcccttgg atttacgcgt gaccctccaa aac
//
LOCUS       DBNGZA010000357         7868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_69820, whole genome shotgun sequence.
ACCESSION   DBNGZA010000357 DBNGZA010000000
VERSION     DBNGZA010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_69820"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="AC16FU_07850"
     CDS             <1..2255
                     /locus_tag="AC16FU_07850"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR2994318.1"
                     /translation="AYYVAAADFVTTEEGTGVVHTAVMYGADDYRLGEELDLPKVHTV
                     DDDGKFKLEVTLWAGQFVKDAEPSIITDLNSRGLLYKKEKTTHTYPFCWRCESPLLYY
                     ARQSWYLKTSQFREQLLKNNRLIHWYPPEIGQGRFGEWLENNVDWALSRERYWGTPLP
                     IWLCEKCDCQQAVESRSHLQKLSGADSFRLDLHKPFIDQVTFPCPKCRATMRRTSEVI
                     DAWFDSGSMPYAQWHYPFESKEKFKAGFPSDFISEGIDQTRGWFYSLLAISTLLFDQP
                     AYKNVIVIELILDKEGQKMSKTKGNVVDPWEVLNREGADALRWYLVSTSQPWLPTRFN
                     REEIVEVQRKLLSTLRNVYAFFALYANLDRFDPAQADLKRAVLTELDRWILSRSNSLV
                     REVTESLNGYDITGAARLISDFVIEELSNWYVRRSRRRFWVSGEPKDKRAAYATLHTI
                     LLSTVKLCAPFIPFLAEELYRALVCRSAGEPESVHLARFPVVDARWIDSALEARMQAA
                     MQLVALGRAARNKSKVRIRQPLPAMKVVLPGGQAQIDLGSLAEVVQEELNVKQITRVA
                     DVEELMTVTAKPNFALLGPKFGQQVKRVAERIRALTTAELRKLQADGVLTIEHNGARL
                     ELDKRDVEITELARGNFAIERNAGYAVALDLTLTEALVDEGLAREVVNRIQNMRKEAG
                     FDVSDRITLSLRTDARLAQAIAAHRQYIQQETLAMLWVEHLADPEFQQAWQINGHPAQ
                     IALSRVRQTT"
     gene            2268..2660
                     /locus_tag="AC16FU_07855"
     CDS             2268..2660
                     /locus_tag="AC16FU_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR2994319.1"
                     /translation="MKKKDLEHYEKLLLAKRTELVGEIGSLKKAALDTTIRDSSGDLS
                     GYSYHMADQGTDAMEREKAFLFASKSGRLLYHIDEALRRIKDKSYGVCQECGGEISAA
                     RLEAVPHARLCIKCKEKEEKEKRLEITE"
     gene            2685..3257
                     /gene="lspA"
                     /locus_tag="AC16FU_07860"
     CDS             2685..3257
                     /gene="lspA"
                     /locus_tag="AC16FU_07860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR2994320.1"
                     /translation="MTTRTVKWPWILVAGVVLFDQLTKQIVKASFTLNESRPVLGDFF
                     RLTYIHNTGGAFGVQLGTPLFYLVSSAMIIGILVLYFFTSRAAIGWSGVSLSLVLGGA
                     IGNLIDRIAYGKVIDFLDFEFFDIAIRPCKLLFWQFPGYQLERWPIFNIADSAVTCGI
                     LLLLATTLVIPRKSPSQVTPVSVTPPAQSG"
     gene            3250..4245
                     /locus_tag="AC16FU_07865"
     CDS             3250..4245
                     /locus_tag="AC16FU_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR2994321.1"
                     /translation="MAEPTVSQVVVPPTQVQKRLDLFLTHQFPQHSRSYFARLIRSGQ
                     VLVNGVKAKPAQKTRAGDAVTLQLFPQFEPTLEPEPIPLDIVYEDDDLLVINKPAGLV
                     VHPAVGHWTGTLVHALLHHGQSLSQPAPWRPGIVHRLDKDTSGLLVVAKNDFVHARLA
                     EQLKQRTLSRHYLAIVCGHLPKITGMIDLPLGRSRKERKKIVVTRIKSRPAQTLYEVR
                     RQFSLCDLVGVSLLTGRTHQIRAHLSHLGHPVLGDPEYGGRAKWWSGLSGQKGKRAQE
                     LLTFIDRQALHAEKIAFVHPRTEKKLEFSASPPQDFQRVVQSLEKEVESQTCKPS"
     gene            4230..5378
                     /gene="carA"
                     /locus_tag="AC16FU_07870"
     CDS             4230..5378
                     /gene="carA"
                     /locus_tag="AC16FU_07870"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR2994322.1"
                     /translation="MQALLVLEDGRIFRGEGFGAAGKGWGEVVFNTSLMGYQEILSDP
                     SHQGQIVVMTYPLIGNYGLNHQDFESRRPWVQGFVVKEYCAKPSNWRAEFTLDEFLKK
                     WGIPGIAGVDTRALTRHLRDRGAKMGIISADGTSEKVLVEKARKLPHLTGRDLAREVT
                     TSQNYPWTEPSEWLPQRREVRFNVVAYDYGIKQNILRKLVSYGCRVTVVPAVTPAEEV
                     VALKPDGIFLSNGPGDPEAVTYAVHAVERLVGLKPIFGICLGHQSLGLAMGGRTFKLK
                     FGHHGANHPVKNLLTGKVEITAQNHGFAVDPASLGGTGLTVTHVNLNDGTVEGMKHGE
                     YPVFSVQYHPEASPGPHDADYLFEEFVQSMEQARKPERGTRYALTNAS"
     gene            complement(5362..5835)
                     /locus_tag="AC16FU_07875"
     CDS             complement(5362..5835)
                     /locus_tag="AC16FU_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994323.1"
                     /translation="MSVPQILGLRDLEKVNRGVLRERLKASRKRLSRLQKQKEQVESN
                     LATVTNYVQYLESLRSLTTTIADQKPKEGKHRLVVRRALRAAPRQTMGVVAYEVIRQA
                     GRPLHLKEIVERLQAQGKLRNAKRPIVAVRNTLTRLKKQFRKTKPATFAIIKTRS"
     gene            5951..>7868
                     /gene="carB"
                     /locus_tag="AC16FU_07880"
     CDS             5951..>7868
                     /gene="carB"
                     /locus_tag="AC16FU_07880"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR2994324.1"
                     /translation="MPRRTDLKTILIIGSGPIVIGQACEFDYSGTQACRVLKQDGFRV
                     VLVNSNPATIMTDPEVAEATYVEPLVPEVVEQIIAKERPQALLPTLGGQTGLNLAVAL
                     AEAGVLDRYGVELIGAKLPSIKLCEDRLLFKEAMCKVGLDLPRSDLATSLTQAEKIAH
                     TIGFPVVIRPSFTLGGTGGGIAYNLTELRQIVSLGLELSPAHSVLIEESVDGWKEYEL
                     EVMRDSADQCVIVCSIENLDPMGIHTGDSITVAPAQTLTDKEYQRMRDAALLIMRTVG
                     VDTGGSNIQFAVEPQSGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLEE
                     IPNDITRKTPACFEPSIDYVVVKVPRFTFDKFPEADPVLTTQMKSVGEAMAFGRTFRE
                     ALQKAFRSTEAGYTGLIPCENGNLTLDQLREKLARPHFERLQHLVQAFAAGLSVDEAF
                     RLTKIDSWFLNQIKSIVELDQRLAKTGQTGDLFRPGLNGVGPELLGQAKASGFSDIHI
                     GARLGLSEERVRALCRKWGLQPVFKAVDTCAGEFEAYTPYFYSTYEEEDEIPHSTKSK
                     VIILGSGPNRIGQGIEFDYACVHAVLATKEAGYEAILVNCNPETVSTDYDVADRLYFE
                     PITREDVLNIVEKEK"
BASE COUNT         1704 a         2286 c         2248 g         1630 t
ORIGIN      
        1 aggcctacta cgtcgctgcg gccgatttcg tcaccaccga ggaaggcacg ggcgtcgtcc
       61 acacggcagt aatgtatggg gcggatgact accgtttagg cgaagagctc gatttgccta
      121 aagtccacac ggtcgacgac gacgggaaat tcaagttgga agtgacccta tgggccgggc
      181 agttcgtcaa ggatgcggag ccgtcaatca ttaccgatct caactcgcgg gggctcctct
      241 acaaaaagga aaaaaccacg cacacctacc ccttctgctg gcgctgcgag tcgcctttgc
      301 tctactacgc ccggcagtcc tggtacctca aaaccagcca gttccgcgaa caactgttga
      361 aaaataaccg gctgatccac tggtacccgc cggagatcgg ccaaggccgt ttcggcgagt
      421 ggctggagaa caacgtcgac tgggcccttt cccgcgagcg gtactggggc acacccttgc
      481 cgatctggct ctgcgagaag tgcgattgcc aacaagctgt cgaatcgcgc agtcacttgc
      541 aaaaactttc tggagcagat tcgtttcgcc tcgatctcca caagccgttc atcgatcagg
      601 ttactttccc ctgccccaag tgtcgggcga caatgcgccg cacatcggaa gtaatcgacg
      661 cctggttcga ttccgggtcg atgccgtatg cccagtggca ctacccgttc gagagtaaag
      721 agaaattcaa agccggtttc ccgtccgact tcatcagcga ggggatcgac cagacgcgag
      781 gctggtttta ttcacttctg gctatctcga cgctcctttt tgaccagccg gcctacaaga
      841 atgtgattgt catcgaactg attctggata aagaaggcca gaagatgtcg aagacgaagg
      901 gaaacgtggt cgacccgtgg gaggttttga accgcgaagg agccgacgcc ctccgctggt
      961 acctggtttc gaccagccag ccctggctgc cgacccgctt caaccgggaa gaaatcgtcg
     1021 aagtccagcg caagcttctt tcgaccttgc gcaacgtcta tgccttcttc gccctttacg
     1081 ccaacttaga ccggttcgat ccagcccaag cagacctcaa acgggcggtg ctcaccgaac
     1141 tggatcgctg gattctctcc cgctcaaact cgcttgtacg agaggtgacg gagagtctga
     1201 acggctatga catcaccggg gccgcgcggc tcatttccga ctttgtgatt gaagaactct
     1261 ccaactggta cgtgcggcgc tcgcgccgcc gcttctgggt ctccggggag ccgaaggata
     1321 aacgggcggc ctacgcgacg ctccatacaa ttcttctctc cactgtcaag ctctgcgcgc
     1381 ccttcattcc ctttttagcc gaggagttgt accgggcgct cgtgtgccgg agcgccggtg
     1441 agccggagag cgtccatctg gcacggtttc cagtcgtgga tgcccgctgg attgacagcg
     1501 cactggaagc ccggatgcag gcggctatgc aattagtggc cttggggcgg gcggccagga
     1561 acaaaagtaa ggtgagaatt cgtcagcccc tcccggcaat gaaggtggtg ctgcccggcg
     1621 gccaggcaca aatcgatctc ggcagtttgg ccgaggtggt gcaagaagag ttgaacgtta
     1681 agcagattac ccgagttgcc gatgtagagg aactaatgac cgtaaccgcc aagccaaatt
     1741 ttgcgctcct gggccccaag tttggccaac aggtgaagcg agtggccgag cgtatccggg
     1801 ccctcaccac ggccgagctc cggaagttgc aggccgacgg cgttctgacc attgaacaca
     1861 atggcgccag gctggaactg gacaaaaggg acgtggaaat caccgaactg gcccgaggga
     1921 attttgccat cgagcgcaat gccgggtacg ccgtggcact ggatttgacc ctcaccgaag
     1981 cgctggtgga tgaagggctg gcacgggaag tggtcaaccg gattcagaac atgcgcaaag
     2041 aggcgggatt cgacgtgtcc gaccggatta cgctttcgct ccgcaccgac gcaagattgg
     2101 cgcaagcgat tgccgcccac cgccagtata tacagcaaga aacgctcgcc atgctttggg
     2161 tggagcatct ggccgatccc gagttccaac aggcgtggca gatcaacggc caccccgccc
     2221 agattgccct ttcccgggtg cggcagacaa catagggagc agagacgatg aaaaagaaag
     2281 atctcgaaca ttacgaaaaa ttacttttag ccaaacggac cgaactggtg ggggaaatcg
     2341 ggtcgctcaa gaaagcggcc ctggatacca ccattcgcga ctcctccggc gacctctcgg
     2401 gttactccta ccacatggcc gaccagggaa ccgacgccat ggagcgggag aaagcgttcc
     2461 ttttcgcttc caagagcgga aggctcctct accacatcga tgaagccctg cggcgcatca
     2521 aggacaaatc ctacggcgtc tgtcaggaat gcggcgggga aatttcggcg gcgcggctgg
     2581 aagccgttcc ccacgcacgg ctctgcatca agtgtaaaga aaaagaagaa aaagaaaaac
     2641 ggctggagat aacggagtaa ggttctccgt gccggagcgg ctccgtgact acgagaacgg
     2701 taaaatggcc ctggattctc gttgcgggcg ttgtcctctt cgatcaactg accaaacaga
     2761 tagtgaaggc cagttttacg ctgaatgaat cacgaccggt tctgggtgat ttttttcgct
     2821 tgacctacat ccacaacacc ggcggcgctt tcggcgtgca actcggcact cctctttttt
     2881 acctggtctc ctcggcaatg atcatcggta ttctcgtgct ctactttttc acatcccggg
     2941 ccgccattgg ctggagtggg gtgagcctgt cactggtcct cggcggcgcc atcggaaacc
     3001 ttatcgaccg gattgcctat ggaaaagtga ttgattttct ggactttgag ttcttcgaca
     3061 ttgccatccg gccatgcaaa ctcctctttt ggcagttccc cggctaccag ttggaacgct
     3121 ggccgatttt caacattgcc gactcagcgg taacctgcgg catcctgctt ttgctcgcca
     3181 ccactctcgt tattcccagg aaatcgccga gtcaggtaac acctgtgtcc gtgaccccac
     3241 ccgcccaaag tggctgagcc gactgtttcc caggttgtcg ttccgcccac acaggtccag
     3301 aagcgcctcg atcttttttt aacccaccaa tttccgcagc attcccgctc ctacttcgcc
     3361 cggctgattc gttccggcca ggtgctggtc aacggggtca aagccaagcc ggcgcaaaag
     3421 acccgggccg gtgatgcggt cactctccag ttgttccccc aattcgagcc aacgctggag
     3481 ccggagccga ttcctttgga cattgtctat gaagacgatg accttttagt catcaacaaa
     3541 ccggcgggat tggtcgtaca tccggcggtc ggtcactgga ccggaaccct cgttcacgcc
     3601 ctgctccacc acggtcagag tctgtcgcaa ccggccccct ggcggccggg gatcgttcac
     3661 cggctggata aagacacctc cggccttctg gtggtagcca aaaatgattt tgttcacgcc
     3721 cgcctggctg agcaactcaa acagcggaca ctttcccgcc actacctggc catcgtctgc
     3781 gggcacttgc caaaaataac cgggatgata gatttacctc tcggtcgttc ccggaaagag
     3841 cgtaaaaaaa tcgtggtgac caggatcaaa agccgcccgg cgcagaccct ctacgaggta
     3901 cgccggcagt tttccctctg cgacctggtc ggcgtttcgc ttttgaccgg ccggacgcac
     3961 cagatccgcg cgcacctctc ccacctcggg catccggtgt tgggtgaccc cgaatatggc
     4021 ggccgggcca agtggtggtc gggtctgtcc ggacaaaaag ggaaacgggc gcaggagttg
     4081 ctcaccttca tcgaccggca agcgctccat gcggagaaaa ttgctttcgt gcatccccgg
     4141 acagaaaaaa aattggaatt ttccgcttcg ccaccgcaag attttcagcg ggtcgtacag
     4201 tcgttggaaa aagaagtaga aagccaaaca tgcaagccct cttagtatta gaagacggca
     4261 gaattttccg cggcgagggc ttcggtgccg ccgggaaagg gtggggtgag gtcgttttta
     4321 acaccagtct catgggttac caagagatcc tctccgaccc ctcgcaccag ggccagattg
     4381 tcgtcatgac ctaccctttg atcggtaact acggtttgaa ccaccaggat tttgaatcgc
     4441 ggcggccctg ggtgcaggga tttgtcgtaa aggaatactg cgctaagccg tccaactggc
     4501 gggcggagtt tacgctggac gaatttctga aaaagtgggg catccccggc attgcgggcg
     4561 tcgacactcg tgcgcttacg cgccacctgc gcgatcgggg cgccaaaatg ggaatcatct
     4621 ccgcggatgg cacgagcgaa aaagtactgg tggaaaaagc ccgcaaactg ccgcacctga
     4681 cgggccgcga cctggcgcgc gaagttacca cttctcaaaa ctatccgtgg actgaaccgt
     4741 cggagtggct accgcagcgg cgggaagtgc gctttaacgt tgtggcttac gactacggga
     4801 tcaagcagaa cattctgcgc aaattagtgt cgtacggctg ccgggtgacc gtcgtgcctg
     4861 ctgtaactcc ggctgaggag gtggtggcgc tcaagccgga cgggattttc ctctccaacg
     4921 gtccgggcga tcccgaagcg gtaacctatg cggtccacgc cgtggagcgt ttggtgggtc
     4981 tgaagccgat tttcgggatt tgcctggggc accagagtct cgggctggca atgggcggac
     5041 ggacgttcaa actgaagttc ggccaccacg gcgccaatca cccggtaaaa aatcttttga
     5101 ccggcaaagt cgaaatcacc gctcagaatc acggctttgc ggtcgatccc gcgtcactgg
     5161 gcggcaccgg acttacggtc acgcatgtca atctgaacga cggtacggtt gaagggatga
     5221 aacacggaga atatccggta ttctccgtcc agtaccaccc ggaagcctcg cccggccccc
     5281 acgatgcgga ttatcttttc gaggagtttg tgcagtcgat ggagcaggcc cgcaaaccgg
     5341 agcggggtac ccgctatgcg ctcacgaacg cgtcttgata atcgcgaaag ttgccggctt
     5401 tgttttccgg aactgctttt tgagccgggt cagggtgttc cgcaccgcaa cgatcggccg
     5461 cttggcgttc cggagcttgc cttgggcctg aagccgctcc acgatctcct tgaggtggag
     5521 ggggcggccc gcctgccgga ttacttcata cgcgaccaca cccatggtct gccggggggc
     5581 ggctcgcagg gcgcggcgca ccaccagccg atgcttgcct tctttcggct tttggtcggc
     5641 gatcgtagtc gtcaaactcc gcagcgactc aaggtactgc acgtagttag tgaccgtggc
     5701 caggttggac tccacctgct ctttttgctt ttggaggcgg gacaggcgct tccggctcgc
     5761 cttcaagcgc tcccgcagga caccgcggtt aactttctcc agatcgcgca aacccagaat
     5821 ttgggggacg ctcatcttaa ttcctcctca ttaactgtct acaccatata tatcaatcag
     5881 ggtgtaaggt caagagagct gtttctatcc agcgcctctt ctgtaggagt cgataagccg
     5941 ttcagatccg atgccccgac gcactgacct taaaaccatc ctcatcatcg gctccgggcc
     6001 gatagtcatc ggccaggcct gcgagtttga ctactcaggc acccaggcct gccgggtgct
     6061 gaagcaggac ggtttccggg tcgtcctggt caactccaac ccggccacca tcatgaccga
     6121 tcccgaagtg gctgaggcta cctacgttga gccactggtg ccggaggtcg tagagcaaat
     6181 tatcgctaaa gagcgtcccc aagctctcct cccgacattg ggcggacaaa ccgggctcaa
     6241 tctggcggtg gcgctggctg aagcaggagt actcgaccga tacggcgttg agttaatcgg
     6301 tgcgaaatta ccctcgatta aactctgcga agatcgactg ttatttaaag aagcgatgtg
     6361 caaagtgggc ctcgacctgc cccgttccga tctggctact tccctcactc aggcggagaa
     6421 aatagcacac acgatcggtt ttccagtggt gattcgcccc agtttcaccc taggcggcac
     6481 cggcggcgga attgcctaca acctcaccga actcaggcag attgtttcgc tgggtttgga
     6541 attgagcccg gcgcattcgg ttttgatcga agaatcggtg gacggctgga aagagtacga
     6601 attggaggtg atgcgcgact ccgccgacca gtgcgtcatt gtctgctcga ttgaaaactt
     6661 agacccgatg gggattcaca ccggtgattc gatcaccgtc gcgccggccc agacgctcac
     6721 agataaagag taccagagga tgcgggatgc ggcacttttg attatgcgca ccgtgggggt
     6781 tgataccggc gggtcgaata ttcagttcgc ggtggaaccg caatccgggc ggatggtcgt
     6841 cattgaaatg aacccgcgtg tttcccggtc gtcggcatta gcctcgaagg ccaccggttt
     6901 ccccatcgcc aaaatcgcgg caaagttggc ggtcggctac acccttgagg aaattcccaa
     6961 cgacattact cggaagactc cggcttgctt tgaaccctcc atcgattacg tggtggtcaa
     7021 ggtgcctcgc ttcaccttcg acaagttccc ggaggccgac cccgtgctga ccacccagat
     7081 gaagtcggtg ggcgaggcga tggccttcgg ccgaaccttt cgggaggcgc tgcagaaagc
     7141 cttccgctcc accgaagcag gctatacggg gttaatcccg tgcgagaatg gtaatctcac
     7201 acttgaccag ttgcgcgaaa aactggcccg gccgcacttc gagcggttgc agcatctggt
     7261 gcaggccttt gccgcgggac tttcggtcga cgaagcattc cggctgacaa agatcgattc
     7321 ctggtttctc aaccagatca agtcaatagt agagctcgat caaaggttag ccaaaacggg
     7381 gcaaaccggt gacctcttcc gtccaggact aaatggggtc ggacccgaac tcttggggca
     7441 ggccaaggcc agcggtttct ccgacataca catcggagcg cgattgggac tctccgaaga
     7501 aagagtgcgc gcactgtgcc ggaaatgggg cctccagccg gtcttcaaag cggtcgatac
     7561 ctgtgccggg gagttcgagg cctacacgcc gtatttctac tccacctatg aagaggaaga
     7621 tgagattccc cactcgacga aatcgaaagt gatcattctt ggttccgggc cgaaccggat
     7681 cgggcaggga attgaattcg actacgcctg cgtccatgca gttttggcca cgaaggaagc
     7741 cgggtacgaa gcgattctgg tcaactgcaa cccggaaacg gtttcgaccg attacgacgt
     7801 ggccgaccgg ctctactttg aaccgatcac ccgcgaagat gtgctgaata tcgttgaaaa
     7861 agaaaaac
//
LOCUS       DBNGZA010000358         2967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_386913, whole genome shotgun sequence.
ACCESSION   DBNGZA010000358 DBNGZA010000000
VERSION     DBNGZA010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_386913"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1852
                     /locus_tag="AC16FU_07885"
     CDS             413..1852
                     /locus_tag="AC16FU_07885"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2994325.1"
                     /translation="MTERVIRCLVLIGLLSLPPAVGEANDSVTVRILTGSHTIQVTTL
                     TFTIPGMGMVSVSPLPPVQGPGDSTFGFYAVADTPTAVSMPFLMDGVGHSFSANPLRY
                     DTLYTLPFHGSPDSKIMFRVRHPDNPPVLAAIGSKNVMEGQMLQFVVTATDPDPSPTL
                     ILSALNRPPNAAFVDSGNGHGLFTFNPDFNQAGMHPVLFVVSDGQKADSEQVTIQVQD
                     VDRPPVLAATGPQTVAEGQVLQVPISASDPDAEPLALIAQNLPVHATLTDSGGGRGLL
                     RFAPDFDQAGPYQVTIIARETRAIGPLADTGAVNINVTNTNRPPVLAAIGPQAIAEDD
                     TLLFAVNALDPDGQSVTLAAFNLPPNALFVDSLNGRGSFRFTPDTTQQGVYDVLFTAT
                     DGTASDSQTVSISVCDISAAPVLADVNCDGVVDITDIVFLINYVVFGAPAPVRAGTAD
                     VNCDLVVDLVDIIATIQNVVFGTPFTGCP"
     gene            1940..2305
                     /locus_tag="AC16FU_07890"
     CDS             1940..2305
                     /locus_tag="AC16FU_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994326.1"
                     /translation="MQRKVFSLVIAVLVLGGFVAGPSRAQLPPPVCKQALEEVQKTNQ
                     MLQRAAPVIRDCPNDTAKFLFRRAVQVQGAAIMALRSRHCVLALDLTTHARRVALKAL
                     SFCGPPGEALPPSGADPGE"
     gene            2453..>2967
                     /locus_tag="AC16FU_07895"
     CDS             2453..>2967
                     /locus_tag="AC16FU_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994327.1"
                     /translation="MGTATATAAAPTTKRTILPAASMGMNDSDPQAIRRARTEEARGF
                     FDFIAQDFPPLSTTREAVEKRLQAGWLLDYVPSGLTFKKPFYDIRNFYSLSLWFLKSK
                     PDPDSPPYADPPTPGRWLFYPREMFIPGSGLKIKPEQEVFLKEKFPGGKWVWPEAGEI
                     TYLLTLNQKRTG"
BASE COUNT          601 a          921 c          846 g          599 t
ORIGIN      
        1 cggcccgaag actcgcccca ggattggatg gcctgatggt aggccaaaat ccctgcggtt
       61 ccttcgcccg ctcccagcca gaagagacgg tttttgtgat ttcgcccgat ttcactccat
      121 cccattcggg gaaccgggga atcagttgcg aagaggggat cccgcgtctg caggcttccc
      181 agagagaacg aaaccgtgga aaaggggaga ggggtctggg cgcaaaaagt cggcacccct
      241 acggtggata ggaataagag aacagcccac actccccgcc gcatgaggga aaggaaaggc
      301 attttccgca ggaggtcaag gaacatttgg cggaggtttt tggttagaga aagtgaagcc
      361 cgcgtggccg gcctttactc gagagaaagg gatttcatcc caggagggga agatgaccga
      421 gagagtgatt cgttgccttg tcctgattgg actcctctcg cttccgccag cggttgggga
      481 agccaatgac agcgttaccg tgcgcatcct gaccggttcg catacgattc aggttaccac
      541 gcttaccttc accatccccg gaatggggat ggtgtcggtg tcgcctttgc cgccggtgca
      601 ggggcccggc gattccacct tcggcttcta cgccgtcgcc gatacgccca cggcggtgag
      661 tatgcccttc cttatggacg gggtgggtca ttccttttcc gccaatccgc tcaggtacga
      721 taccctctac accctgccct tccacggcag tcccgactca aaaattatgt tccgggtgag
      781 gcatccagac aatccgccgg ttttggcggc gatcgggtcg aaaaacgtca tggaaggaca
      841 aatgctccag ttcgtcgtga cggccaccga tcccgaccca agcccgaccc ttatcctctc
      901 cgccctcaac cggccgccga atgcggcgtt tgtcgatagc ggtaacgggc acggcctttt
      961 caccttcaat cccgatttca atcaggcggg gatgcacccg gtgctgttcg tggtttctga
     1021 cggccaaaaa gccgacagcg aacaggtgac aattcaggtt caggatgtcg accgtccgcc
     1081 ggttctggcg gccacaggcc ctcagacagt ggccgagggg caggtgttgc aggttccgat
     1141 cagcgccagc gatcccgatg ccgagccgct ggccttaatt gcacagaatc tcccggtgca
     1201 cgcgaccctg accgacagcg gcggaggccg cgggctcttg cgtttcgctc ccgattttga
     1261 tcaggccggc ccgtaccagg tcaccatcat cgcccgagaa accagagcca taggaccttt
     1321 ggccgatacc ggagcggtga acatcaacgt aacgaacacc aaccgccccc cggtgcttgc
     1381 cgccatcggc ccccaggcga ttgccgaaga cgacacgctc ctttttgccg tgaacgccct
     1441 tgaccccgac ggccagagcg tcactctcgc cgcgttcaat ctgcctccca acgccctgtt
     1501 cgtcgatagt ctcaacggcc ggggctcgtt ccgcttcacc ccggatacga cccagcaggg
     1561 ggtttacgac gtgctcttca ccgcgactga cggcaccgcg tccgacagcc aaacggtttc
     1621 gatttcagtc tgcgacattt ccgccgcgcc ggttctggcc gacgtcaact gcgacggcgt
     1681 cgtcgacatt accgacattg tcttcctcat caactacgtc gtctttggcg ctccggcgcc
     1741 ggtccgggcc ggaaccgccg acgtcaactg tgacttagtc gttgatctgg tcgacatcat
     1801 cgccaccatc cagaacgtgg tttttggaac gccttttacc ggctgtccgt aaaaacgctt
     1861 aaacctttcc ttcgctcctt tggtctgaaa tactgtagac agtgaaacgc aaccatttca
     1921 gatcagggag gtgatgagga tgcaaaggaa ggtgttttcg ctggtaatag ccgtactcgt
     1981 cctcgggggt tttgtggccg gccccagccg ggcccagctg ccgccgccgg tctgcaagca
     2041 ggcgctggag gaagtacaga agaccaatca aatgctccag cgggcggcgc cggtgattcg
     2101 ggattgcccc aacgacacgg ccaaattcct gttccgcagg gcggtgcagg tgcagggtgc
     2161 cgcgattatg gccttgcgca gccgtcactg tgttctggcg ctcgatttga ccacgcatgc
     2221 ccggcgcgtg gcgctcaaag ccttatcttt ctgcggaccg cccggagagg cacttccgcc
     2281 gtcaggcgcg gatcccggag aataatcgtt cgttcctcaa ggaaatgtga taaaagcgtc
     2341 ccgtcaaact acggcgggac gcttttcgtt ggcttgacaa ccacctcccg gcaaactata
     2401 taaaaccgtc tgagaggccg aaataagcgt tatcggtaaa cacggagcac ttatgggaac
     2461 agcgactgct actgctgctg cgccgacaac caaaaggacg atcctgccag cagcgtcaat
     2521 ggggatgaac gattccgatc cgcaggcgat tcgccgggcg cgcaccgaag aggcccgggg
     2581 tttttttgac ttcattgcgc aggattttcc tccgctttcc acaacccgcg aggcggtcga
     2641 aaagcgcctg caggcgggct ggcttctcga ctacgtcccg tccggattga ccttcaaaaa
     2701 gccgttctac gacatccgga acttctattc gctttccctc tggtttttga agagcaagcc
     2761 cgaccccgac tccccgccgt acgccgaccc gcccactccg ggccgctggc tcttttaccc
     2821 gcgggaaatg ttcattccgg gaagcggatt gaaaatcaaa ccggagcagg aggtcttcct
     2881 gaaggagaag ttccccgggg gaaaatgggt ctggcccgaa gcgggggaga ttacctacct
     2941 cctcaccttg aaccagaaac gcaccgg
//
LOCUS       DBNGZA010000359         5911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2826394, whole genome shotgun sequence.
ACCESSION   DBNGZA010000359 DBNGZA010000000
VERSION     DBNGZA010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2826394"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16FU_07900"
     CDS             <1..180
                     /locus_tag="AC16FU_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994328.1"
                     /translation="GGYQVCEKWLKDRKGRRLSLEEIKHYCNIVTALQKTIEVQKEID
                     AIYPQVEQDVIQFGK"
     gene            532..930
                     /locus_tag="AC16FU_07905"
     CDS             532..930
                     /locus_tag="AC16FU_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR2994329.1"
                     /translation="MTKFYNKSSEKEKRSRLRNQMPAAEVILWSRLQKRQILGFKFRR
                     QYSVGPYVLDFYCPEVKLAVEIDGDSHFREDASEYDDNRQMSIEELGVRFLRFTNLEV
                     YKNLTGVLKSITGTIEKVGKQAGRKTKRGK"
     gene            1283..2704
                     /locus_tag="AC16FU_07910"
     CDS             1283..2704
                     /locus_tag="AC16FU_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR2994330.1"
                     /translation="MPDPLDLFEKEKPQKRGISQPAEHPLADRMRPEVLEEFLGQEHI
                     LGKGKPLQVAIEQDRLGSMIFWGPPGSGKTTLARIIAKKTQAHFVAYSAVTAGIKEIK
                     ELMAEVEKREQLYHQRTILFIDEIHRFNKAQQDAFLPYVEKGIIILIGATTENPSFEV
                     NAPLLSRCRVFVLHPLSIDDIKTILNRALGDAVRGLGQRRLDVSPDALAYIAQIASGD
                     ARVALTALEFIASTLAPKSRGAVPSTSLGPARDKSLRTSKMDLKTAQAALQKKAFLYD
                     KAGEEHYNLISALHKSLRDSDPDGALYWLARMLASGEDPLYVARRMIRFASEDIGNAD
                     PQALSITLAAKDAYHFLGTPEGELALAQACLYLATAPKSNAVYAAFNAVNDDLKDMEA
                     QPVPLVIRNAPTNLMKAVGYGKGYQYAHDNPEHFADQEHLPENLAGRRYYLPTDFGFE
                     KGIKARMERWQKLREELRKKSGQ"
     gene            2715..4265
                     /locus_tag="AC16FU_07915"
     CDS             2715..4265
                     /locus_tag="AC16FU_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR2994331.1"
                     /translation="MWDVRRAGQGPVARKKDHRKGGNFRSACSRTAVFLGLFGTLATV
                     SFAKPPYEPGDWVSYTNERYVRSVVYAYEWVYFGTTEGIARLNRFNHQWGDPITTSDG
                     LPDNSVLELAYDPTQDALWAATRLGTASFQSTLRQWYLAPRFPPDVKPKSKPPQNPLP
                     NFYIDFNYTLYPEGVIQDQFLRRYPVRTSLEDDMGSIWYGTWGLGVLEGTPRDQRATL
                     RHFGLWSSVVTAMAIDGDFVWFGGTRAFTQPSGITRWNRQTQEWTYYEAPYTDGLISD
                     NINAIVADSQYVWFGTELGLVRYDKKRNRFKSYTRRQRVADEEVTALDVDSNYLYVGT
                     TNGVSILDKKKDSLWVENAGLPPATVVNAIKSTGDDLWIGTRDGLFRKTKGQPKWVRY
                     LDPTRILSAEVRAIVRAGDELWFGSQIGVVRFNLVTQKTSAFEQGAGTGRLQVYGLAV
                     DSLQAFIAVPGGVWRWQRGGDFWRLLSTNDGLIDPTVYQMVWDGDYLWFATNKGATRF
                     YWKTRRIE"
     gene            4322..5302
                     /gene="thiL"
                     /locus_tag="AC16FU_07920"
     CDS             4322..5302
                     /gene="thiL"
                     /locus_tag="AC16FU_07920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR2994332.1"
                     /translation="MPVGEFELIDFIRKKFAPSKKRGAVVLGVGDDAAALRLAGDKLL
                     LVTTDTVVEDVHFKLRYFTYEQVGHRIMAANLSDIAAMGGWPTYYLVTIGLPKGTTQK
                     NVDAIYRGMERLARKHRVALIGGDIVRAEKLFVTVTLLGEVERKNLLTRSGAHRGNLV
                     CVTGHLGASLLGFRLLNQKSAKRVPHRLTRRHLQPEARLAEARWIVQNLRPTAMIDIS
                     DGLSSELNHLARESRVGFAVEAGKIPVPPGVRRAIGNLKGNWVDYALASGEEYELLFT
                     VPKSQVARLKNWPKHLAPVSVIGRVAAKGVVLIGPEGRVEKIRPSGWRHL"
     gene            complement(5359..5781)
                     /locus_tag="AC16FU_07925"
     CDS             complement(5359..5781)
                     /locus_tag="AC16FU_07925"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR2994333.1"
                     /translation="MDNKKKIIEMLNEDLGDELASIIQYMWHHVMVAGPTAPGAKEEF
                     MKAARDEMKHAEMLAEKVNYLGGVPTTRHGEIRVGGDLKKMLNDNLEGELRAIARYKK
                     QIASAAVESEPAIRHMLEEILEDEEDHADIWQTMLEGL"
BASE COUNT         1335 a         1638 c         1703 g         1235 t
ORIGIN      
        1 ggcggttatc aagtttgcga aaaatggctg aaggacagga aaggtagaag attgtcgttg
       61 gaagaaatca aacattattg caacatcgtc accgctctgc aaaagaccat tgaggttcag
      121 aaagagattg acgcgattta tccccaagtc gaacaggatg taattcaatt cgggaaatag
      181 aaaaacgaag ggacaggccg acgtgactgc tccgggcggg cacgaggttc gtccccgtga
      241 ccccctgcgg cctgaaagcc gcgtccccct tacgtcaggg ggacaaagtg accccctggg
      301 cctgaaagcc gcgtcccctt tacgtcaggg ggacaaagtg accccctggg cctaaaggcc
      361 ccgtccccct tggcaagggg gacagaaata gcaaagcaga aactttttgt tccccttttt
      421 tcaaggggga cgcacagggg gtcgagtttt cctccttact taagggcgac ccacagggag
      481 tagtttttgc tcctttgaag agagggactt tagagggtat attttaactg aatgaccaaa
      541 ttttacaata aatcttccga gaaggagaag cgcagccggc tgcgaaatca aatgcccgcg
      601 gcagaggtca ttctctggtc gcggctccag aaaagacaga ttttgggatt caaattccgg
      661 cggcaataca gcgtcgggcc gtacgttttg gatttctact gcccggaagt gaaattagcg
      721 gttgaaatcg acggtgacag ccattttcga gaagacgcct ccgagtatga cgacaaccgg
      781 cagatgtcaa tcgaagaact tggagtacga ttcctccgtt tcaccaacct cgaagtttac
      841 aaaaatctta ccggcgtttt gaagtccata accggaacga tcgaaaaagt cggaaagcag
      901 gcggggagaa aaacaaaaag gggaaagtga ccccctgggc ctacaggctg tgtccctctt
      961 aattagaagg acaaaccgac cccctcggcc taacggccgt gtcccccttg taaaggggga
     1021 cagaaatagc aaactagaaa ctttttgtcc cccttttttc aagggggacg caccgccaaa
     1081 ggcgggcagg gggtagttgt aacctgaccc cctgggcaaa aggccccgtc ccccttgtaa
     1141 agggggacag aaatagcaaa ctagaaactt tttgtccccc ttttttcaag ggggacctac
     1201 aggggggcga aagacaaacc gaacgggaaa aataaagcag acatttttga aaggttggtc
     1261 tcaccgcgta tatttatcag tgatgcccga tcccctcgat ctctttgaga aggaaaagcc
     1321 ccaaaagcgc ggtatctccc agcccgctga gcacccttta gccgaccgga tgcgtccgga
     1381 ggtcctggag gagttcttag ggcaggagca cattttgggc aaagggaagc cgctccaagt
     1441 cgccatcgag caggacaggt tgggctcgat gatcttctgg gggccgccgg ggtcaggcaa
     1501 gaccacgctc gcccgcatca tcgccaaaaa gacgcaagcg catttcgtgg cctactcggc
     1561 agtgaccgcc ggcatcaagg aaatcaagga gttaatggcg gaggtggaga agcgggagca
     1621 gctctaccac cagcgcacga ttctcttcat cgacgaaatc caccgcttca acaaagccca
     1681 gcaggatgcg tttctgccct acgtggaaaa agggataatc atcctgattg gcgccaccac
     1741 cgaaaatcca tcgttcgaag tcaacgcccc gcttttatcg cgctgccggg tttttgtgct
     1801 ccatccgctg tcgattgatg acatcaaaac cattctcaac cgggcgctcg gtgatgcagt
     1861 gcggggactt ggtcaacgca gattggatgt ttcccctgac gctctcgctt acattgccca
     1921 gatcgcctcc ggcgatgcgc gggtggcgtt aacggctttg gagtttattg cctccacgct
     1981 ggcgccaaaa agtaggggcg cggtcccttc gacttccctc ggccctgctc gggacaagtc
     2041 gctcaggaca agcaagatgg atttgaaaac cgcgcaggca gcgctccaga aaaaagcctt
     2101 tctgtacgac aaggcgggcg aagagcacta caacctcatt tccgccctgc acaaatcgct
     2161 gcgcgattcc gatccggatg gagcgctcta ctggctggcg cggatgctcg cttccggcga
     2221 agaccccctc tacgtggcgc ggcggatgat ccgcttcgcc tccgaagaca tcgggaatgc
     2281 ggatccgcag gcgctttcca tcactttagc cgccaaggat gcgtaccact ttctgggaac
     2341 accggaaggg gaactggcgc tggcgcaggc ctgtctctat ttagccacag cgcccaagag
     2401 caatgcggtt tatgcggcct tcaatgccgt gaacgacgac ctgaaagata tggaagccca
     2461 gccggtgcct ttggtcatcc gcaatgcgcc gacgaatttg atgaaagccg tcgggtacgg
     2521 gaagggctac cagtacgcgc acgacaaccc ggagcacttt gccgaccagg agcacctgcc
     2581 ggaaaatctg gcgggaaggc gctactacct gccgacggac tttggatttg aaaaaggaat
     2641 taaagcgcgg atggaacgct ggcagaagtt gcgcgaggaa ctaaggaaga aaagtggcca
     2701 gtagccctag aaatatgtgg gacgtacgtc gggcaggcca ggggccagta gccagaaaga
     2761 aagaccacag aaaaggcggg aattttcgtt ctgcctgcag ccgaacggca gtcttcctcg
     2821 gccttttcgg cacgctggca acggtctctt tcgccaaacc gccctacgag ccgggtgact
     2881 gggtaagtta caccaacgag cgctatgtgc gctccgtcgt ctacgcctac gagtgggtct
     2941 acttcggcac caccgagggg attgcccggc tgaaccgctt taaccaccag tggggcgacc
     3001 cgatcaccac ctccgacggc ttgccggaca acagcgttct ggaactggcc tacgatccga
     3061 cgcaggatgc cttgtgggcg gccacccgcc tggggaccgc ctccttccag agtacccttc
     3121 gccagtggta tctcgccccg cgctttcctc cggatgtgaa accgaaaagc aagccgcccc
     3181 aaaatcctct gccgaacttt tacatcgatt tcaactacac cctctatcct gagggggtga
     3241 ttcaggacca gtttctccgg cgctatccgg tccgcacttc gctcgaggat gacatgggtt
     3301 ccatctggta cggcacctgg ggactgggcg ttctggaggg aaccccgcgc gaccagcggg
     3361 ccacgctgcg acattttggg ctctggtcgt cggtcgtgac ggccatggcc attgacggcg
     3421 attttgtctg gttcggcggt acgcgggctt ttacccaacc cagcggcata acccgctgga
     3481 accgtcaaac acaggagtgg acctactacg aggcgccgta caccgacggc ctcatctccg
     3541 acaacatcaa cgcgattgtg gccgattccc aatacgtctg gttcggcacc gagctgggcc
     3601 tggtgcggta cgacaagaaa aggaaccggt tcaaaagtta cacccgccgg cagcgagttg
     3661 ccgatgagga agtgaccgct ttagacgtgg acagcaatta cctctacgtc gggacgacga
     3721 acggcgtctc gattttggac aaaaagaaag acagtctctg ggtggagaac gccggtttgc
     3781 ccccggcgac tgtggttaat gccatcaaat cgaccggcga cgacctctgg atcggtaccc
     3841 gcgacggcct cttccgtaag acgaaaggcc aacccaagtg ggtgcgctac ctcgatccga
     3901 cccggattct ctccgccgaa gtgcgcgcca ttgtgcgtgc gggggacgag ttgtggttcg
     3961 gctcgcagat cggggtggtg cggttcaatc tcgtgacgca gaaaacctcc gcgtttgagc
     4021 agggggcggg caccggacgg ctgcaggtgt acgggctggc ggtcgattcg ctccaggcct
     4081 tcatcgcggt gccgggcggg gtctggcgct ggcagcgggg cggcgacttc tggcggttgc
     4141 tttccacgaa tgatggtcta atcgacccga ccgtctacca gatggtctgg gatggcgatt
     4201 acctctggtt tgcgacgaac aagggcgcaa cgcggttcta ctggaaaacg cggcggatag
     4261 agtaaactca ggtacaaaag tttgactttc aaagtttgtc cggcagcggt atttttactt
     4321 tatgccggtg ggcgaatttg aactgattga tttcatccgc aaaaagtttg ccccttccaa
     4381 aaagcgcggg gcggtcgttt tgggcgtggg ggatgacgcg gcggcgctgc gtttagccgg
     4441 agacaaactc ctcttagtca ccaccgacac ggtggtggaa gatgtccact tcaaactccg
     4501 ctacttcacc tatgagcagg tcgggcaccg gattatggcg gccaatctct ccgacattgc
     4561 cgcaatgggc gggtggccga cctattacct ggtaacaatc ggtttgccaa aagggacgac
     4621 gcaaaaaaac gtcgatgcaa tctaccgcgg gatggagcgt cttgctcgca aacatcgggt
     4681 ggcgctcatc ggcggggata ttgtgcgggc agagaaactt ttcgtgacgg tgacgctttt
     4741 aggcgaagtt gaacggaaga atttgctcac gcgttccgga gcgcacaggg gcaatctggt
     4801 ctgcgtcacc ggccacttag gtgcgtctct cttagggttt cggttgctca atcaaaagtc
     4861 agctaaaaga gtgccgcatc gtttgacccg gagacacttg cagccagagg cccgtttagc
     4921 cgaagctcgc tggattgtgc aaaacctccg gcctacggcc atgattgaca tttccgacgg
     4981 gctctccagc gaactcaacc atctggcgcg ggagagccgg gttggatttg ccgtagaggc
     5041 gggaaaaatt ccggtgccgc ccggtgtccg gcgggcaatc ggaaatttga aagggaactg
     5101 ggttgactac gcgctggctt caggcgagga gtatgaactg ctttttaccg ttccgaaatc
     5161 acaagttgcc cggctcaaga actggccaaa gcatctggcg cctgtttccg tcataggccg
     5221 ggtggcagct aaaggagtcg tacttattgg tccggaaggc cgcgtggaaa aaattcgacc
     5281 gagcggctgg cggcatttgt aaacaaaaaa agggcgagtg aactcgcccc tcccggatcc
     5341 gttggtatgg atgatcgctc acagcccttc caacatcgtc tgccagatgt cggcgtggtc
     5401 ctcttcgtcc tccaagattt cctccaacat atggcggatg gccggctcgc tctctacggc
     5461 cgcggaggca atctgcttct tgtagcgggc aatcgctctt aactcgccct ctagattatc
     5521 gttgagcatc tttttcaaat caccgccgac gcggatttcc ccgtgcctgg tcgtagggac
     5581 gccgcccagg tagttgacct tctcggccaa catttcggcg tgcttcattt cgtcccgcgc
     5641 ggctttcata aactcctctt tggcgcccgg agccgtgggg ccggcgacca tcacgtggtg
     5701 ccacatgtac tggataatcg aggccaattc gtcgcccagg tcttcgttca gcatctcgat
     5761 tattttcttt ttgttgtcca tttttcctcc aggaaatcgg ttgactttcc gccgccaaca
     5821 tactttgttg ctcagcgacg gtcaaccgct tttatgggct ttttggcggc gactgcgaca
     5881 taaagaatac caaaaccatc ggcggaattt t
//
LOCUS       DBNGZA010000360         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2826405, whole genome shotgun sequence.
ACCESSION   DBNGZA010000360 DBNGZA010000000
VERSION     DBNGZA010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2826405"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..437)
                     /locus_tag="AC16FU_07930"
     CDS             complement(168..437)
                     /locus_tag="AC16FU_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR2994334.1"
                     /translation="MPIYEYRCQSCSTRFEIFFRSAAEPAACPQCRGTALTRLFSRFA
                     VVRREAAGDDFGDVPDSSSAPDFGEDSGADACPPEGCGHCQDNLD"
     gene            complement(440..1336)
                     /gene="sppA"
                     /locus_tag="AC16FU_07935"
     CDS             complement(440..1336)
                     /gene="sppA"
                     /locus_tag="AC16FU_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR2994335.1"
                     /translation="MARKREWIIGSIIGASFLVFVLLFFIGISSLLRQPGDSDFSGFG
                     PKVAVIDITGVITTAGDVVRQLKKYRSDASVKAILLRIDSPGGGVAASQEIYSEVKKV
                     RDEDKPVVVSMGSVAASGGYYIACAADSIVANPGTLTGSIGVIFEFPVAEQLLKKIGL
                     KWEVVKSGEMKDVGTFTRPLTEKERRHLQGVIDDTYDQFVKVLVENRKLPRRKVLALA
                     DGSIFTGRQAQKLGLVDQLGDYEDAVKLAGRMGGIAGEPRTVKERKRRPSWLDLLTET
                     KNLLQQFTPAGESGPRLLYQFK"
     gene            complement(1349..>1831)
                     /locus_tag="AC16FU_07940"
     CDS             complement(1349..>1831)
                     /locus_tag="AC16FU_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR2994336.1"
                     /translation="LAGRYGTSVKSIVRVNKLGKGNRLRVGRRILIPVVSGRHPSYAR
                     QGTPRPPLAADGTYRIRRGETLTEIAQRHNVSLAELQQVNGLSDPHTVKAGARLKIPP
                     KSLPERIGDSAAVYVVRAGDTLWEIAKKFGITIQQLVVWNPAVDPDNLPVGTRLEIRK
                     "
BASE COUNT          341 a          589 c          531 g          370 t
ORIGIN      
        1 ccgcttccaa aaacgaatcg acgaccacgg ccacgtcggt ccgggtcagt cccgtctagc
       61 cggctacctg ctcaaccaaa tcggctttgg tcatgctccc tcctgaaatc ctggcttaga
      121 tcgcttatcc acaaaatcgg tcacaatggc ggctgatttt ttcacgctca gtcgagattg
      181 tcctggcaat gcccgcagcc ttccggcggg caggcgtccg ctccgctatc ttctccgaag
      241 tcgggcgcac tgcttgaatc cggaacgtca ccgaagtcgt cgccggcggc ttcacggcgc
      301 accacggcga aacgagagaa taaacgggtt aaagccgtcc cccggcactg gggacaagcc
      361 gccggttccg cggcgctacg aaagaagatc tcaaagcggg tgctgcaact ctgacagcgg
      421 tattcgtaaa tcggcatcgc tacttgaact ggtagaggag ccggggaccg ctctcccctg
      481 cgggggtgaa ctgttgcaac aggttcttgg tctccgtcaa caggtcgagc caggagggcc
      541 ggcgcttgcg ctccttcacg gttctcggtt cgccggcaat accgcccatc cggccggcta
      601 atttcacggc atcctcgtaa tcccccagtt ggtcgaccaa acccagcttc tgggcctgac
      661 ggccggtgaa gatcgaaccg tcggccaagg ccaggacttt gcgccggggg agctttcggt
      721 tttccactaa gaccttcacg aactggtcgt aggtgtcatc aatcacgccc tgcaggtggc
      781 ggcgctcttt ttcggtaagc ggacgggtga aggtacccac gtccttcatt tcacccgact
      841 ttacgacctc ccacttcaag ccgattttct tcaacagttg ctcggcgacc ggaaattcga
      901 agatgacccc gatggagccg gtgagggtac cgggattggc gacgatggaa tcggcggcgc
      961 aggcgatgta gtagccgccg gaggcggcca ccgagcccat ggagactact accggcttgt
     1021 cttcgtcccg caccttcttg acttcgctgt agatttcttg cgaggccgcc acgcccccgc
     1081 cgggactgtc gatgcgcagg aggatggctt tcaccgaggc atccgaccgg tactttttca
     1141 gttgccggac gacgtcgccc gccgtagtga tcacgcccgt gatatcaatg acagcgacct
     1201 tggggccgaa gcccgagaag tcggaatctc cgggctgccg taggagggat gagatcccga
     1261 tgaagaacag gagcacaaaa acgagaaagc tcgctccgat gatgctcccg atgatccact
     1321 ctcttttccg cgccattctc agcgattcct attttctgat ctcaagccgg gttcccaccg
     1381 gcaggttgtc cgggtccacc gccgggttcc agacgaccag ctgctggatc gtgatgccga
     1441 attttttcgc gatctcccag agcgtatcac ccgcccggac gacgtaaacc gccgccgaat
     1501 cgccgatccg ttccggcagg ctcttcggcg ggatttttag ccgcgctccc gctttgacgg
     1561 tgtgcgggtc gctcaggcca ttgacctgct gcaactcagc cagggaaaca ttatgtcttt
     1621 gggcgatttc cgtcaaggtc tcgccgcgcc ggatccggta ggtgccgtcg gcggccagcg
     1681 ggggacgcgg ggtaccctgg cgggcgtaac tcgggtgccg acccgaaacg accggaatca
     1741 aaatccgccg gccgacgcgc agccggttcc ctttgcccag cttgttcacc cgcacgatcg
     1801 acttcacgct cgtcccgtac cgcccggcca g
//
LOCUS       DBNGZA010000361         1224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2188968, whole genome shotgun sequence.
ACCESSION   DBNGZA010000361 DBNGZA010000000
VERSION     DBNGZA010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2188968"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="AC16FU_07945"
     CDS             complement(<1..411)
                     /locus_tag="AC16FU_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994337.1"
                     /translation="MFKKLTSLGCFGLALLVLAVGCSKSTNPMKSGSATATVILKVKG
                     SSSTGLAKPAAAAVALTEAQVVIHRLRFDGAEDKDEVDFRLREPLIVNLNLGGAEQIL
                     GEFKVTPGTFDRTRFRIEKLDDGDGQVFAANPGLQ"
     gene            complement(637..912)
                     /locus_tag="AC16FU_07950"
     CDS             complement(637..912)
                     /locus_tag="AC16FU_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR2994338.1"
                     /translation="MPKYVIERDIPGAGKLSKGELQAISQKSCSVLSAMGPQIQWLHS
                     YVTDNKIYCVYIAPNEKAVREHAQKGGFPANRISEVHTLIDPTTAEP"
     gene            complement(976..>1224)
                     /locus_tag="AC16FU_07955"
     CDS             complement(976..>1224)
                     /locus_tag="AC16FU_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR2994339.1"
                     /translation="IAKQAEAERERRAKVIHAEGELQASYKLAEAAEVIGKQPAALQL
                     RFLQTLVEVAADKNSTTVFPVPIDLIKPFMTLAERGGK"
BASE COUNT          254 a          338 c          364 g          268 t
ORIGIN      
        1 ctggaggccc ggattagccg caaacacctg accgtctccg tcatctaatt tttcgattcg
       61 gaagcgggta cggtcgaaag tgcctggagt gactttaaat tcccccagga tctgctcggc
      121 accgccaagg ttgagattga cgataagcgg ctcacgcaag cggaagtcaa cttcgtcctt
      181 gtcctcggca ccgtcaaacc ttaggcggtg gatgaccact tgggcttcgg tcagagccac
      241 tgcggcagcc gcgggtttgg ctaagccggt cgacgatgag cccttaacct tcaggatgac
      301 cgtagcggtg gccgagccgg atttcatcgg attggttgat ttgctgcatc cgaccgccag
      361 caccagcaga gccaggccga aacagccaag agaggtgagt ttcttgaaca tagacaacct
      421 ccttactaat aggtctctta cctatctaac cgctactgcg cagattttgt tcccgaagac
      481 ggggaggggg gaggtaaatg tgaaaatcga agtgagcctc agacccccac gggaggaaat
      541 ttccagcgga accagagagc gccgcctaca acagatgggg gaaaccgctt cataggacgg
      601 cgaagtccgc cgccggtagt gacgtctgaa acgagcttaa ggttcagcgg tcgtcgggtc
      661 gatcagggtg tgcacttccg aaatccggtt ggccgggaag ccgcccttct gggcatgctc
      721 cctcacggct ttttcgttcg gcgcaatgta gacgcagtaa atcttgttgt cggtgacgta
      781 actgtgcaac cactgaatct gcggacccat agcgctcaag acggagcagg acttctggga
      841 aatggcctgg agctctcctt tggacagttt tccggcgcca gggatgtcgc gttcgattac
      901 gtatttgggc attgggtcct cctctgtttt acgcccacag gggctaagct gcaaaattaa
      961 atgcgcgggt ttgggctact tccctccccg ctccgccaat gtcatgaacg gtttgatcag
     1021 gtcgatgggg acggggaaca ccgtcgtcga attcttgtcg gcggcaacct cgaccaaagt
     1081 ttgcaaaaag cgcagttgca gagcagcggg ctgcttgccg atcacctcgg ccgcttcggc
     1141 caatttgtag gaggcctgga gttcgccttc ggcgtggatg accttggcgc ggcgctcgcg
     1201 ctccgcctcg gcctgcttgg caat
//
LOCUS       DBNGZA010000362         3172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_387978, whole genome shotgun sequence.
ACCESSION   DBNGZA010000362 DBNGZA010000000
VERSION     DBNGZA010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_387978"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="AC16FU_07960"
     CDS             <1..229
                     /locus_tag="AC16FU_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994340.1"
                     /translation="VIRFSLPHRGEIELAVYNILGQKVKTLAAGQWEAGRYQITWDGT
                     DAQNHPLASGVYFYQLRSPDYIEAKRMLLVK"
     gene            complement(267..1496)
                     /gene="rodA"
                     /locus_tag="AC16FU_07965"
     CDS             complement(267..1496)
                     /gene="rodA"
                     /locus_tag="AC16FU_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR2994341.1"
                     /translation="MRLSLIRSPEFDRWLTVDALFLSLLGVVLIYSATHTAIDPGEHQ
                     LPWKQLLWLPVAFFGFFAAFFLPLRLHEALAYVYYAAGAILLAAVLILGETRMGATRW
                     FAFGTFNLQPSELAKITAVFGLARLFAHAREPVTGLRRLVVAVVMILLPVGLILRQPD
                     LGTALVFCALGLAMLFWSGLPLWHLALLVSPLFSLLAAFHWITWALFFVLLLWVLYKT
                     RPGFLFGTICLVANLLFGMVTPLVWNRLHDYQKMRILIFLDPGRDPQGAGYQLIQAKV
                     AIGSGGIFGKGFLHGSQTKLAFLPAQHTDFIFTVVGEEFGLLGVLIVLALLGFLVYRG
                     LQAAIRCRNQFASYVALGLTSILAFQILVNVGMAAGLMPVTGLPLPFLSYGGTSLLTN
                     WVIIGLLASIAVHWQDY"
     gene            1560..1988
                     /locus_tag="AC16FU_07970"
     CDS             1560..1988
                     /locus_tag="AC16FU_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994342.1"
                     /translation="MNQTTTQPSLVRSVRAAGIVVLCALGATFSITQCSKKTIKASLF
                     IFRGTVTAKESGNLLRNVEILLNGNSRDTTDASGRYSFAEEIRTDRQLNAVPVFDSLP
                     DYTQCGTGRFSVPAGDTVWITNFGLCRRPPAPPCTCTVSR"
     gene            complement(2004..>3172)
                     /locus_tag="AC16FU_07975"
     CDS             complement(2004..>3172)
                     /locus_tag="AC16FU_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR2994343.1"
                     /translation="PEPARPGSDLFLTIDLDIQAVAESALAGSCCGAAVALDPRTGEV
                     LAFVSKPGFDANAFVTGLSLDQWQKVSGDSTHPMLNRTIQGLYPPGSTVKLLTAGAAL
                     ESHLITPAKRFNACQGALWFGNRLFRCWKPEGHGSLDLYQAIVQSCDVYFYQLGLALG
                     LERWGEYAAKCGFGQKTGIDLTDEWAGVLPTAAYYARRFGQNQWPKGVMLNLSIGQGE
                     FLVTPLQLATFFGALGNDGAVFRPHLLKRYVDPDGLEREIEPVCVRQLPFSPGTLRFL
                     KTALVGVVNDPHGTATVAQLPGISVAGKTGTAQNPHGLEHAWFACFAPAEKPEIAVAV
                     LVENAGHGGTVAAPIAREMLRAYFTKTHPLLQPEVAASAGVSDRSRRPATPGGD"
BASE COUNT          735 a          970 c          932 g          535 t
ORIGIN      
        1 cgtcattcgc ttctcactcc cccaccgagg cgagatcgaa ttggcggttt acaacattct
       61 ggggcagaag gtcaaaacgc tcgccgccgg tcagtgggaa gccggacgct accagatcac
      121 gtgggacggt accgacgcgc agaaccatcc gctcgcttcg ggggtgtact tctaccaact
      181 ccggtcgccc gactacatcg aggccaagcg gatgctgttg gtgaagtaaa ctgtcgcggt
      241 ggggaaattc cttacaaccg aataggtcaa taatcctgcc agtggacggc gatactggcc
      301 aaaagcccga tgatgaccca gttggtcaaa agcgacgtgc cgccgtagct caaaaagggg
      361 agcggcaggc ccgtcaccgg catgagcccc gcggccatac cgacgttgac cagaatctgg
      421 aaggcgagaa tcgacgtcag ccccaaggcc acgtacgacg caaactggtt gcggcagcgg
      481 atggcggcct gcaaaccgcg gtagaccagg aatcccaaaa gggccaacac gatcaaaacg
      541 cccaagaggc cgaattcttc ccccaccacc gtgaaaatga aatcggtgtg ctgggccggc
      601 aggaaagcca gcttggtctg gctgccgtgc agaaaacctt tgccgaatat gccgccggaa
      661 ccgatcgcca ccttggcctg aatcaactgg taaccggctc cctgcgggtc gcgccccggg
      721 tctaagaaaa tcagaatgcg catcttctgg tagtcgtgga gccggttcca gaccaaaggg
      781 gttaccatgc cgaagagtag attagccacc aggcagatgg tcccgaagag aaacccgggc
      841 cgggttttgt acaataccca gagcagcagg acgaaaaaca aagcccaggt gatccagtga
      901 aaagcggcca aaagactgaa gagcggagaa accaaaagcg ctaagtgcca gagcggcagt
      961 cccgaccaga agagcatggc caaccccagt gcgcagaaga ccagagccgt gcccaaatcg
     1021 ggctggcgca gaatcaaccc gaccggcaga agaatcatga ccaccgccac caccaagcgg
     1081 cgcaggccgg tcaccggttc gcgggcgtga gcgaacagcc gggccaatcc gaacaccgcc
     1141 gtgatcttcg ctaactccga cggctgcagg ttaaaagtcc cgaaggcaaa ccagcgggtg
     1201 gcgcccatgc gcgtttctcc cagaattaac actgccgcca gtaaaatggc ccccgctgcg
     1261 taatagacgt aagccagcgc ctcgtgcagg cgtagcggca ggaagaaggc agcgaaaaat
     1321 ccgaaaaacg ccaccggcag ccagaggagt tgtttccacg gcaactggtg ctctccgggg
     1381 tcgatggccg tatgggtggc gctgtaaatc agcaccaccc ccaatagaga aagaaagaga
     1441 gcgtcgacgg tcaaccagcg gtcgaactca gggctgcgga taagtgacaa gcgcatggcc
     1501 gcttacagta aaccggtctg tttgctccct ggagaaacgg gaattatctt catcctgcta
     1561 tgaaccaaac gaccacgcaa ccaagcctcg tgcggtcggt ccgggccgca gggatcgtgg
     1621 tgctctgcgc tctgggcgca acattttcta ttacccagtg ttcgaaaaaa acgatcaagg
     1681 ccagtctctt catcttccgg ggcacggtaa ccgccaagga atccgggaat ctcctgcgca
     1741 atgtggagat tcttttaaac ggcaactcgc gcgacaccac cgatgcctcc ggacgctact
     1801 ctttcgccga ggaaattcgc accgaccgcc agctcaacgc agtcccggtg ttcgattcac
     1861 tgcccgatta cacccaatgc ggtacggggc gcttttcggt ccccgccggc gacacggtct
     1921 ggattaccaa tttcgggctc tgccgccggc cgcccgctcc accctgcacc tgcacggtga
     1981 gccgctaaac gtaacccgcc aattcaatca cctccggggg tggccggacg ccggctgcgg
     2041 tctgagactc cggcactggc cgctacttca ggttggagca gggggtgtgt cttggtaaaa
     2101 taggctctga gcatttcccg ggcgatgggg gcggccaccg tgccgccatg cccggcattt
     2161 tccaccaaca cggcgacagc gatctccggt ttctctgccg gagcaaaaca ggcaaaccag
     2221 gcgtgctcca acccgtgggg attctgggcc gttccggttt ttccggcaac gctgattccc
     2281 ggaagttgtg caaccgtggc ggtaccgtgc gggtcgttta ccacgccgac cagggcggtt
     2341 tttaagaacc gcaaagtccc ggggctgaaa ggcaactgac gcacgcagac cggctcgatc
     2401 tctctttcca gtccgtccgg gtcaacgtac cgcttcaaaa gatgcggccg gaacactgcc
     2461 ccatcgtttc ccaaggcacc aaagaaggtg gccagttgca agggcgtgac caagaactcc
     2521 ccctgaccga tggacaggtt gagcatcacc cctttgggcc actggttctg gccaaaccgg
     2581 cgagcgtaat aggcggctgt aggcaagacc ccggcccact catcagtaag gtctatcccg
     2641 gtcttctggc cgaagccgca cttggcggcg tactcacccc accgttccag gcccaaggcc
     2701 aatcccagct ggtaaaaata gacatcgcag gattggacga tcgcctggta caggtccaga
     2761 ctgccgtgcc cctccggttt ccagcagcgg aacagccggt ttccgaacca cagggcaccc
     2821 tggcaggcat taaaacgctt ggcgggggtt atgagatggg attccagagc cgccccggcg
     2881 gtcaaaagtt tgacggttga ccccggcggg tacagtcctt gaatggtgcg gttcaacatg
     2941 gggtgtgtgg aatcgccgga gactttttgc cactgatcca gtgaaagtcc agtgacaaac
     3001 gcgttggcat cgaatcccgg tttggacaca aaggcgagaa cctcacccgt ccgggggtcg
     3061 agggcgaccg ccgcgccgca gcacgaaccg gccagggccg attcggctac ggcctgaatg
     3121 tctaaatcga tggtcaaaaa gagatcgctg ccgggccggg ccggttccgg ag
//
LOCUS       DBNGZA010000363         1228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1872040, whole genome shotgun sequence.
ACCESSION   DBNGZA010000363 DBNGZA010000000
VERSION     DBNGZA010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1872040"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="AC16FU_07980"
     CDS             <1..650
                     /locus_tag="AC16FU_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994344.1"
                     /translation="RAEVLAPAGDINHDGYPDLLTGYPLGTNFLTGFAYVYFGGPNMD
                     NKADVTINEADLPVPAPDFGQAIAGVGDINGDGIDDFIVGAEEGIADVDDRGQVWVFA
                     GDSTLRPTAVTEIPSATPIRFSRLVQNYPNPFNPQTTIEYELKADEPLATRLIIYDIL
                     GRVVRTLVDEVQRTGRYRVTWDGKDDAGHEVASGVYFYQLKAGNYTEAKKVVLLK"
     gene            complement(655..951)
                     /locus_tag="AC16FU_07985"
     CDS             complement(655..951)
                     /locus_tag="AC16FU_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994345.1"
                     /translation="MVKACAPGGRVESRPEALVIQDVLETAGIPVVVRTPDKYLLLQG
                     DWRGSPKPLDTSLRLYDRESHRPPVEIWVAGDRVDEAKALLAAESGLAGEGNPF"
     gene            983..>1228
                     /locus_tag="AC16FU_07990"
     CDS             983..>1228
                     /locus_tag="AC16FU_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR2994346.1"
                     /translation="MGKNKSRNSLTSVLFGDTRRAILALLYGHPDESFYFSQVVRAAG
                     IGHGTVQRELDRLARAGILRRVVRGREVHYQANPDCPI"
BASE COUNT          272 a          356 c          344 g          256 t
ORIGIN      
        1 agcgggccga agtgctcgca ccggcaggcg atatcaacca cgacggctac cctgatctgc
       61 taacgggata ccccttgggc acgaattttt tgaccggttt tgcctacgtc tactttgggg
      121 gcccaaatat ggacaacaaa gccgacgtga ccattaatga agcagactta ccagtccccg
      181 ctcctgactt tggccaggcc attgctgggg tgggcgatat caacggcgat ggaatagatg
      241 actttatagt tggagcagag gagggaatcg ctgacgttga tgatcgggga caggtgtggg
      301 tctttgccgg agacagtact ctgcgaccta ctgccgtgac cgagattccc agcgcgactc
      361 cgattcgctt ttctcgattg gtacagaact accccaaccc gttcaacccg cagaccacga
      421 tagagtatga attgaaggca gacgaacctt tagccacccg cctgatcatc tacgacatct
      481 tgggccgcgt ggtgcgcacg ttagttgatg aagtccagcg caccggccgc taccgcgtga
      541 cctgggatgg aaaagatgac gcggggcacg aggttgccag tggggtttac ttctatcaac
      601 ttaaggccgg aaactacacc gaagcgaaaa aagtggttct gctgaagtag ttcttcaaaa
      661 tgggttgccc tcaccagcca atccgctctc ggcggcgagc agtgctttgg cttcgtcaac
      721 cctatctccc gccacccaga tttccaccgg cggtcgatgc gactctctgt catataaccg
      781 cagactcgtg tcgagcggtt tgggcgaacc gcgccaatcg ccctgcaaaa gcaggtactt
      841 atcgggcgtc cgcaccacca ccggaatccc ggcggtctct aagacgtcct gaatgactaa
      901 cgcctcaggc cggctttcca cccggccgcc gggggcacag gccttgacca tcttacccaa
      961 aatgggtaaa ttcttaccca aaatgggtaa aaacaaaagc cggaattcgc tgacgtcagt
     1021 tctctttggc gacacccgcc gggcgatttt ggccctcctc tacggccatc cggacgaatc
     1081 cttttacttc agccaggtgg ttcgtgcggc cggaatcggc cacggcacgg tgcaaaggga
     1141 actggaccgg ctggcccggg cgggaatttt acgccgggtg gttcggggaa gagaggttca
     1201 ttaccaggcc aatccggact gcccgatt
//
LOCUS       DBNGZA010000364         5725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_388398, whole genome shotgun sequence.
ACCESSION   DBNGZA010000364 DBNGZA010000000
VERSION     DBNGZA010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_388398"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1290
                     /locus_tag="AC16FU_07995"
     CDS             241..1290
                     /locus_tag="AC16FU_07995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015482379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylasparaginase"
                     /protein_id="HIR2994347.1"
                     /translation="MSPNLVEEDKTLTRRNFLTGVSALVGSAAIAEVGLAELLAEKTH
                     PPRRVTSKVVKVRLPVIISTWPQGLAATKIAGELLIAGKSGLDAVEKGINTAEDDPKV
                     QSVGLGGLPDENGIVTLDASIMDGPRRTGGAVGGLENIRNAISVARKVLEDTDHVLLV
                     GDGAKQFAIACGFKEENLLTEESRKAWLEWKKSGQKGFWGRPPDQFGHDTIGLILCDA
                     NHNLYGGVSTSGLSWKIHGRVGDSPILGAGLFVDNDVGAACATGRGEEVIKVCGSFLI
                     VELMRQGHSPQEACEEALRRIRRNNPKRTDFAEAFLAVNKDGETGAAYFGKEFTYAIF
                     APDVHEMRSGEKWEH"
     gene            1409..3355
                     /locus_tag="AC16FU_08000"
     CDS             1409..3355
                     /locus_tag="AC16FU_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994348.1"
                     /translation="MRVPKLLLTATLSLLALPAIYGERSRAIAAVPQLINFQGKLTDV
                     TGNPVADGVYTLTTKIYDDSTAGTVLWTEVRPVQVQGGLFTVQLGSSVALSGNLFSSG
                     TNRFLGVTVNFDPEIAPRTRLISVPYAYQALRADTAITASVALSVAGGPFLPLAGGTM
                     TGPITNTGGPSITMGKANFGVGNNNGGINAFVAGANNMASGNNSAVVGGLNNAASGDV
                     STVGGGTGNTAGGDGSTVGGGVSNSASGYAATVAGGQVNTASGAARATVGGGYSNTAS
                     GTQATVSGGVLNMASGQSATIGGGQSDTASAFGVTISGGNSNLASAFAATIAGGQYNK
                     ARGQFSVVGGGGGFALADSNAALAEASAIGGGKRNIVSGDYAVIGGGRLNFARGQFSV
                     VNGGGGGLVFASDSNSALGDWSVIGGGAANVASGQFSTVGGGTFNTASNNRATVSGGH
                     FNVASGGFAASVGGGERDTSSGSWSVVPGGFGNNAAGDYSFAAGQRAKAVHAGSFVWA
                     DNNNIDFASTSVNQFLIRAAAVGIGTNSPAAKLDVNGSVAMAGPVSIGGGAQISSVLS
                     ASATLNFPSTPTQTSSDLTITVTGASPGDAVSLGVPNASVLADSHFSAWVSAANTVTV
                     RFNNYSATTQDPASGIFSAAVIRF"
     gene            3415..3750
                     /locus_tag="AC16FU_08005"
     CDS             3415..3750
                     /locus_tag="AC16FU_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR2994349.1"
                     /translation="MKRNIRVVDGREILEKVTQLSIKRWSFESQDPQIEHIGPMAQDF
                     YALFGVGDDDKTISTIDPAGIALAAIQELHKENEALKARVSELERLVQTLLTERQDKS
                     GDYTLAAGR"
     gene            3838..5415
                     /locus_tag="AC16FU_08010"
     CDS             3838..5415
                     /locus_tag="AC16FU_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR2994350.1"
                     /translation="MPTRGERVHLKTTCLFATVLTFLFALPARADQLRITNYESRALT
                     PPCSLQQVYRSQADTADQHWGHAVSGLGDVNNDGVPDFIIGVHAADPNGIAEAGEVIV
                     FSGATNLPLYQVAGADTGNEFGSTLAAVGDLNGDGKPDFVAAAPSAERFGALSAGTAG
                     LYSGVNGALIRSFVGDSADNQSGLPTQFGFSAANAGDVNNDGKNDIIIGAPFADPGGS
                     ADAGAAYVFSGTTGARLFKFTGPQAGVWFGNSVAGLGDITGDGKSEFAVGAPFTGTDS
                     GAVFVYSGTGTLLYIKKGQAGDQLGWSLAKAGDIDGDGKADLIAGAPFSDIGVTNGGG
                     AFVLSGPTGNVIRQLPGLVTNGFAGYSVCGLADFDGDAIPDLAVGAILDPTGGVFNAG
                     RVSVYSGATGELLYERIGDAVIRELGYSVADAGDLNADGKSELLTGTPSARPNNQPFA
                     GVAFVFTAPPFRGDVTGDAVHDISDIVALVRFVVFGDPVAVPNAADANCDGVTDIVDI
                     VGLVQFVVFGSPKPCCL"
BASE COUNT         1145 a         1746 c         1702 g         1132 t
ORIGIN      
        1 aagtagaaat agcactttat tcccctcttc gaattatttc aaaaggttca taacagattt
       61 cacggccacc acccaacaac ataccttaac ccctccctgc ccctcccctc aaaggggagg
      121 gattcgatct ttccctctcc tgtaggagag ggtcggggtg aggtcaacac tgcgggcgcg
      181 gcgaccagtt tgacttccct ttctgtcagg atacttttac gcaaaacaga ggaggttccc
      241 atgtccccta atcttgtcga agaagacaaa accctcaccc gccgcaactt cttaactggc
      301 gtttcggctc tggtcggttc tgccgcaata gccgaggtcg gcctggcgga gttgctggcc
      361 gaaaaaactc accccccgcg tagagtcacg tctaaagtag tcaaagtcag actcccggtc
      421 atcatctcca cctggccgca ggggttggcc gcaacaaaga ttgcgggcga actcctgata
      481 gcagggaaaa gcgggttgga tgccgtcgag aagggcatca acacggcgga ggatgacccg
      541 aaagtacaga gcgtcggcct gggcggcctg ccggacgaga atggcatcgt cacgctggac
      601 gcctcgatta tggacggccc ccggcgcaca ggcggcgcgg tcggcggatt ggaaaatatc
      661 cgcaatgcga tctccgtcgc ccggaaagtg ctggaagata ccgaccacgt gctcctggtc
      721 ggtgacggcg caaaacagtt cgcgattgcc tgcggcttca aagaggaaaa tcttttgacc
      781 gaggagagcc gcaaggcctg gctggaatgg aaaaagtcgg gccagaaagg cttctggggc
      841 cgtccccccg accaatttgg acacgacacc atcggcttga ttctctgcga cgcgaaccac
      901 aacctctacg gcggcgtttc caccagcggg ctttcgtgga aaattcacgg ccgggtgggc
      961 gattccccga tcctcggggc cgggcttttt gtggataacg acgtcggagc cgcctgcgcc
     1021 accggccggg gcgaggaagt gatcaaagtc tgcggcagtt ttttgatcgt cgaactgatg
     1081 cgccaggggc attcgcctca agaggcctgc gaggaagccc tgcgccgtat ccggcggaat
     1141 aatccgaaaa gaaccgactt tgccgaggcg tttctggcgg tcaataaaga cggcgaaacc
     1201 ggcgcggcct actttggcaa ggaattcacc tacgctatat ttgctccgga cgttcacgaa
     1261 atgcggagcg gggaaaagtg ggagcactga caacggattt gccaacggat taaacggatc
     1321 gaaggaactt gtaggggcga acttcatgtt cgcccagaat tgttttcaaa tccacctggt
     1381 caccaacgat ccacagagga ggtttaccat gcgcgtaccg aaactgcttt tgacggcgac
     1441 cctcagtctc ctggcattac cggccattta tggcgagcga agtcgagcca ttgccgccgt
     1501 accccagctc atcaacttcc agggcaaact gaccgacgtc acggggaacc ccgttgccga
     1561 cggcgtctac accctcacca ccaaaatcta cgacgactcc accgccggaa cggtcctctg
     1621 gaccgaagtc cgcccggtgc aggtgcaggg cgggctcttc accgtgcaac tgggctcaag
     1681 cgtcgcgctt tccggtaatc tcttttccag cggtaccaac cgtttcctcg gcgtaaccgt
     1741 caatttcgac ccggaaatcg ctccgcgaac tcgcttgatt tcggtgccct acgcctacca
     1801 agccctccgc gctgacacag ccatcaccgc cagtgtagca ctctctgtgg ctggcggccc
     1861 ctttctgccc cttgccggcg gcaccatgac cgggcccatc accaacaccg gtggcccatc
     1921 aataacgatg ggaaaagcca acttcggtgt gggaaacaac aatgggggga taaacgcttt
     1981 tgtggctgga gcaaacaaca tggccagcgg gaataactcg gcggtcgtcg gcggccttaa
     2041 taatgccgcc agcggtgatg tctcaaccgt gggcggcgga acgggcaaca ccgccggcgg
     2101 tgatggctca accgtgggcg gcggagtctc caatagcgcc agcggttatg cggcaacggt
     2161 cgccggcggg caggtcaaca ccgccagcgg cgccgcgcgg gccacggttg gcggcggcta
     2221 tagtaatacc gccagcggca cacaggccac ggtcagtggt ggggtactaa acatggcgag
     2281 cgggcagtca gcgacgattg ggggtggcca gtcggatacc gccagcgctt ttggcgtcac
     2341 catcagcggc ggcaacagca acctggccag tgcctttgcg gctaccattg ccggcggaca
     2401 gtacaacaaa gcccggggcc agttctcagt tgtcggcggt ggcggcggat tcgccctggc
     2461 tgattccaat gcggcactgg cggaagcatc tgccattggc gggggtaaaa ggaatatcgt
     2521 cagcggcgac tatgccgtca tcggcggcgg gagactcaac tttgcgcgcg gccaattttc
     2581 cgtggttaat ggaggtggcg gcggactcgt ctttgcgtca gattccaact ccgctcttgg
     2641 ggactggtcg gtcattggcg gaggggccgc caacgtcgcc agtggtcaat tctcaaccgt
     2701 cggcggcggc accttcaaca cggcaagtaa caaccgcgcc acagtcagcg gcggccactt
     2761 caacgttgcc agcggcggtt ttgccgcctc cgtcggcggc ggagaaagag acacctcgag
     2821 tgggtcatgg tctgtcgtcc cgggaggttt tggaaacaat gccgccggtg attactcttt
     2881 tgctgccggc cagagggcca aggccgttca cgccggttcc ttcgtctggg ccgataacaa
     2941 caacattgat ttcgcttcca ctagcgtcaa tcagttttta atccgcgcgg ctgccgtggg
     3001 cattggaacc aacagcccgg cggccaagct ggacgtaaac ggaagcgtgg ctatggccgg
     3061 accagtgtcg attggcggcg gcgcgcagat ttcttcggtg ttgagcgcaa gcgcaactct
     3121 gaattttcca agtacaccca cgcaaaccag ttcagatttg acgattaccg tgacgggcgc
     3181 atcccctggc gatgccgttt ccctcggcgt gccgaacgcc agcgtgcttg ccgactcgca
     3241 tttttcggcc tgggtttcgg ccgccaacac ggtaaccgtg cgctttaaca actattcggc
     3301 aacgacacaa gacccggctt ccggcatctt cagcgcagcg gttataagat tttgaagagt
     3361 cgaacataag gagcgacctg ggcttctgcc tgggtggcgg tttcggacag caatttgaaa
     3421 cggaacattc gggtggtgga tggcagagaa atactggaaa aggtgacgca gctctccatc
     3481 aagcgctgga gttttgagtc ccaagacccg caaatcgagc acatcggccc gatggcgcag
     3541 gatttctacg ccctcttcgg cgtgggggat gacgacaaga ccatttccac catcgatccg
     3601 gcgggcatcg ctctggccgc cattcaggaa ctgcacaagg agaacgaggc gctcaaagcg
     3661 cgggtgagcg aattggagcg tctcgtgcag acgcttttga ccgagcggca ggacaagtcc
     3721 ggcgattaca cgttggctgc cggacggtag ctggcagcgg atttgcaaac gggtggaact
     3781 tttcctcctc ctctcctgtt ttacaggtga aagcagactg tccgcaggag gaggtttatg
     3841 ccaaccagag gagagcgtgt gcatctcaag acgacgtgcc tgttcgcaac ggttctcacc
     3901 tttcttttcg cccttcccgc ccgcgccgac caattacgaa ttaccaatta cgaatcacga
     3961 gcgctgactc ccccctgctc cctccagcag gtctaccgct ctcaggccga caccgccgac
     4021 cagcactggg gccacgcggt ttccgggctg ggtgatgtga acaacgacgg tgttccggac
     4081 tttatcatcg gcgtccacgc ggctgacccc aacggcattg ccgaagcggg cgaggtaatc
     4141 gtcttctccg gcgccacgaa cctcccattg taccaggtgg caggtgcgga taccggcaat
     4201 gaattcggct ccactctggc ggcggtaggc gacctcaatg gcgacggcaa gcccgacttt
     4261 gtggccgccg cgccttcagc ggaaaggttc ggcgccttga gcgccggaac ggcgggactc
     4321 tattccggag tgaacggtgc gctcattcgt tcctttgtgg gcgattcggc ggataaccaa
     4381 tcgggcctgc cgacccagtt cggtttttcc gcggccaatg cgggggacgt caataacgac
     4441 gggaaaaatg acatcatcat cggtgcgcct tttgccgatc ccggtggttc ggcggatgcc
     4501 ggcgccgcct acgttttttc cggcaccacg ggcgcgcggc tcttcaaatt taccggcccg
     4561 caggccggag tgtggttcgg caacagtgtg gcgggtttgg gtgacatcac cggtgacggc
     4621 aaatccgaat tcgccgtggg cgcacccttt accggcacag actccggcgc cgtcttcgtc
     4681 tattcgggta ccggcacgct cctctacatc aaaaaaggtc aagccggcga ccagctgggc
     4741 tggtctttag cgaaagcggg agacatcgac ggcgacggca aagccgacct catcgccgga
     4801 gcacctttct ccgacatcgg cgtaaccaac ggcggcggcg ccttcgtcct ctccggcccg
     4861 accggaaatg ttatccgtca actcccgggc ctggtgacca acggctttgc gggctattcc
     4921 gtgtgcggcc ttgccgattt cgatggagat gccattcccg atctggctgt gggagcgatc
     4981 ctggatccga ctgggggcgt cttcaacgcc ggccgggtct ccgtttactc gggcgccacc
     5041 ggcgaactgc tctacgagcg cattggcgac gcggttatcc gcgaactggg ttattcggtg
     5101 gccgatgcgg gagacttgaa cgccgacggc aaatcggaac ttttgaccgg cactccctcc
     5161 gcgcggccca ataaccaacc cttcgcgggt gtggcctttg tctttaccgc tccccccttt
     5221 cgcggcgacg tgaccggcga cgcagtgcat gacatctccg acatcgttgc cctcgtccgc
     5281 ttcgtcgtct tcggcgatcc ggtcgccgtc ccgaatgccg ctgacgccaa ctgcgacggc
     5341 gtcaccgaca ttgtagatat cgtgggcctg gttcagttcg tcgtcttcgg cagccccaag
     5401 ccctgctgtt tgtaaacacc ggctggcatt gtataacgta ggggcgaggt tacctcgccc
     5461 ggggtctgtg aaggaacagt cggtacgatc ctacggatcc gtatggaccc gtacgggctt
     5521 accgccagcc agcaggcgag gtcattaaga attcctcact aatccctgaa ctctgatccc
     5581 ccgccactgg cgtggtcgta tcgacccaat ctccgttttt cagaggcaaa tcggcaccgg
     5641 cccgccgaat accacgtact gcaccaaccc cacgatgtcg gcaatgtcca cgatgctgtc
     5701 gccgttgacg tcgccggccg ttaag
//
LOCUS       DBNGZA010000365         7714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1130206, whole genome shotgun sequence.
ACCESSION   DBNGZA010000365 DBNGZA010000000
VERSION     DBNGZA010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1130206"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1808)
                     /locus_tag="AC16FU_08015"
     CDS             complement(201..1808)
                     /locus_tag="AC16FU_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994351.1"
                     /translation="MPPRLPAVAGGDVYVQDGGETSIGINYDDGNNLVAGYNTWPWNL
                     DSHSNSTDGNLTWTSRTFPNGAGIFTGWTADPWTNAGNAAGEFFSTRLKVTSGFPPPP
                     PFNFDSVHCIVARSTDGGASFSLFFEKFKAIFQDKEAVDIDKTTARGGGAGITHDGKV
                     YLGYDDDGLAPNFSYRGSFLQVISSAGVADTEIQISGTGSPPFRGRYLQPVAGITDGT
                     VYVKGHNFGSTIPAIFHEITNGGAGPNTFSKSSFSYFPAGQALGGSGFWGVNGHRIPT
                     NIFGYLDIDRSSGPRRGYLYFITNRNPNRANPALDQGDVYLSVSTNGAASWSSALIPT
                     AAGKTQYTPMLDVDEQGWIHIAYYQNETGSVNGGVLNASTANVYYTRSTDGGTSWAVP
                     VQVNEPANALDYEDPPPNRSGSGYYLIGDYAQLQATGSGPSTKVYILWTGYDKDRSDA
                     FVGDAKARVWCTTIQCFPRGDVTSDAVTDIVDIVALIQNVVFGDPLPNETAGDVNCDN
                     VRDIVDIVQLIGVVALGQPTPCCFGPR"
     gene            2157..2708
                     /locus_tag="AC16FU_08020"
     CDS             2157..2708
                     /locus_tag="AC16FU_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2994352.1"
                     /translation="MEKPDEELAITQFTQGVPNNYIDGLIRQTVRFQIWGKKFEAEEV
                     IQNTRLALLSCFREGKYKGKGLTTYVCRIAAIQSVMEMRRHYRSEKYRAEWSEQANQQ
                     PGPAGNPLTTLLEREKKDRAIRVLKTLGKLCRQLLMLKFYKGLSYREIGGRLGLTEVN
                     VRVSVHRCLQKSKEIAKKMEEGQ"
     gene            2747..2848
                     /locus_tag="AC16FU_08025"
                     /pseudo
     CDS             2747..2848
                     /locus_tag="AC16FU_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
     gene            2906..4879
                     /locus_tag="AC16FU_08030"
     CDS             2906..4879
                     /locus_tag="AC16FU_08030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994353.1"
                     /translation="MSEEHFPVETLADYAFRVKTAARQTETLNWGEEKIEEHLPVCDR
                     CQAELDKLTAFEKEFQRVSKKSVTEPSLEITPLSRWSLFRSRKVWYAAAAAAALILLL
                     LPTLPLRSQGPMIAVLPFEYTGPAEKEYYVFGVTDEITARLGKVSAIGVIAPSSTMQY
                     KNTAQKPRQIGEELGVLYLLTGSVRWDTTGKIAKLQVVPKLVRTRDQKQLWTEPFEII
                     RGELCNLTGVIARKVIAALNVEFLESERQAMEACPTGNIEAYEYYLRGLEYAKRYEKA
                     GIAIQMYEKAVELDPGFALAYAQLSFCHTFVYWFGDPTEERLSKIKETMERAFQLQPN
                     LPEAHLAMAWYVSMADLDIERILKECALAVKELPNNVDALYITGVMQRCAGRYEVAVD
                     YFKKAARLDPLSPNISLELAHTYLFQHNYREAEREYGRGISVTPDYANFYISEAYFLC
                     LYGEGSIEKARKVLEKAAGKVDSLRLAAIWAVVDLFDGNYQKALSRLSSASSFGPIIR
                     GRTATDYFLMKAELYRLMNQWQLAHIYYDSARIDLEPKAIGHKRPRDAFVHQKLGVAY
                     AGLGRKEDAIREGKKALELRPMTRDARFGANLVEGLAEIYVMVGEYDLAIDQLEYLLS
                     VPSLISVPFLRLDPAWIPLRSHPRFQKLINERA"
     gene            complement(4924..5586)
                     /locus_tag="AC16FU_08035"
     CDS             complement(4924..5586)
                     /locus_tag="AC16FU_08035"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR2994354.1"
                     /translation="MEKKIKIPRPKVRQEWGRDPVTKVKSSEKLYRREELKKIDWEAE
                     REPGEWEMAAGQFVFCPKCSAELVGRRLEGRMRLVCPVAACGFIYYRNPVPAVGAIIL
                     QDGKLLMVKRKFEPQAGKWSLPAGFIEYEESPTACVRREVKEETGFTIEIDRLFNVYP
                     AQDDPRSQVVLIIYLARIVSGRLKPGDDAVKAEFFAPENIPEDVAFTAHRKAIAEFFQ
                     LG"
     gene            complement(5594..>7714)
                     /locus_tag="AC16FU_08040"
     CDS             complement(5594..>7714)
                     /locus_tag="AC16FU_08040"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR2994355.1"
                     /translation="LFFAALGALFAAFGTVLGPVDEKLLARPGVALVNNLFYFWELFF
                     PQLLFFALVFPYERPFVRRFPRLRLVFYLPYLFHVLLVSLFSDPTRLERWFSTENMAA
                     PLRAILEPLAVAGKLLLAFLSLLYQAHVQLFSVVNLVYVAASIWLLWRGYRRLTNPRL
                     KAQVRLIFWGIRGGVGLYALAFLLPALAPALSPGPMLRQLLTIGALVTGAGAVAWGII
                     RYQFMDVKLLVRQSLAYSVTSALVVGLYVLSANQFGRLVKNIFGQKIPLLDVGFIALA
                     IILFQPLFGRIDDLIKRWFIRGTSDFRNLMERFTREIISILDPRELKQTVLEVLEKEV
                     LVERAYLLLREREKPAYLLFAGDPTSARETVLFAEDDPVIAHIGGKSKPFVAELAEPA
                     LRESPLGQFCQRERVRVIVPLLEQKFLVGCLALSDKHSGYRYTAEDFTLLSVLANQLT
                     VALANAELYREHLEKQRLEEELALARQIQFDLLPRFNPRGPEFEFASFSQPSRQVGGD
                     YYDYLLAESKVGVVIADASGKGMPAALLVAMLQATLKAEYRHRKPLAEIVGNVNELVA
                     SSTSPEKFVSFFCGDFEPEKKLFTYTNAGHNHPILLRADGSWEELTTGGLLLGVFPES
                     RYEAGRVALRPADSLIFYTDGLTDAQNQEEEQFGEERLRKLVREHRNLCAEELKDKII
                     TTVNDFCAGAPQVDDMTLVVLKVF"
BASE COUNT         1816 a         2176 c         2140 g         1582 t
ORIGIN      
        1 ccgagcccga cgcctcccga agagagacga gtcgtatccg aggattctat cctcggcgcc
       61 cgtacgggcg tcgcctcagg gcaactcgca caggcggtta accccgagtc cgcctcaggc
      121 ggactggaaa aactccgcaa tcgccttccg gtgcgcgcgg aaggcaatgt cctcagcaat
      181 aagtcagctc tcggtttgag ttacctcggc ccaaaacagc acggggtagg ttgccccaac
      241 gccaccaccc caatcaactg cacaatatcc acgatgtccc tgacgttatc acaattcaca
      301 tcccccgcag tttcgtttgg caggggatcc ccgaacacga cgttctgaat caacgccaca
      361 atgtccacaa tatcggtcac agcgtcgcta gtgacatccc cgcgcgggaa gcactgtatc
      421 gtcgtacacc agacccgcgc tttggcatca ccaacgaaag cgtcgctgcg atccttgtca
      481 tatcctgtcc agaggatgta gaccttggtg gacggcccgg agccggtggc ctgcagttgg
      541 gcgtagtcgc caatgaggta ataaccggat ccgcttcggt tcgggggcgg gtcttcatag
      601 tctaaagcgt tggccggttc gttgacctgc accggcactg cccaactggt gcccccatcg
      661 gttgagcgag tgtagtagac gttcgccgtg ctggcgttca gcactccgcc attcacagag
      721 ccggtttcat tctggtagta ggcgatgtga atccagccct gctcatccac atccagcatg
      781 ggcgtgtatt gagtcttccc cgcggccgta ggaatgagcg cggaggacca gctcgcggct
      841 ccattggtag agaccgaaag atacacatcc ccttgatcca gcgcagggtt ggcgcggttg
      901 gggttgcggt tcgtgatgaa atagaggtag ccccgccgcg gcccggacga ccggtcaatg
      961 tcaagatagc cgaaaatatt ggtgggtatt cgatgtccat ttacgcccca aaagccgctt
     1021 ccaccaagtg cctgacccgc cggaaaatag gagaaagacg acttgctgaa ggtgttcgga
     1081 cctgctcccc cgttggtgat ttcgtgaaaa atggccggga tagtgctgcc aaagttgtgc
     1141 cccttaacat acacggtgcc gtcggtaatg ccggccaccg gctgcaggta gcggcctcgg
     1201 aaaggggggc tgccagttcc ggaaatctgg atttccgtat cggccacccc ggcggaggag
     1261 atgacttgca ggaaggaccc cctgtagcta aagttgggtg caagaccatc atcgtcataa
     1321 ccgagatata cctttccatc gtgcgttatc ccggctcccc ctccccgggc cgtggtcttg
     1381 tctatgtcca ccgcctcttt atcctgaaat atggccttaa acttctcaaa gaaaagcgaa
     1441 aaactggcgc cgccgtcggt cgaacgggcc acgatgcagt gaacactgtc gaagttaaag
     1501 gggggaggag gaggaaaacc gctggtcacc ttaagccgtg tggagaaaaa ttctccggct
     1561 gcgttcccgg cattggtcca gggatcagcg gtccaaccgg tgaagattcc ggcgccattg
     1621 ggaaaggtgc gggaggtcca ggtgaggttg ccgtcggtgg agttgctgtg agaatcaagg
     1681 ttccacggcc acgtattgta tccagcgacg agattatttc catcgtcgta attgatccct
     1741 atggaggttt ccccgccgtc ctgaacgtag acatcgccac ctgcaaccgc cggtaagcga
     1801 ggaggcacca cctcccccgg ccggaacacc ggtgcacgcg gtttgatggg tacccagttc
     1861 cccgcgctgt cctgaacaaa cagcttgccc aagaaaagtg gcgggcctgg ctttacctct
     1921 gtcggcggag gcgttgcagg ctccgggaaa aaggtggttt tgagaagttg ctgggcactg
     1981 gcgcaaggga cttcaattgc gaaagccaga agcccggcaa cggctaccca aaaaagtctt
     2041 tgccggttca taccacacct ccgattgaaa agtttcagtc ttcgcttata tagtagccga
     2101 ccggttgctt ttgttacaga aaaacccaat tacggtgcta tattgaccca aagcttatgg
     2161 agaaacccga cgaagagtta gctataactc aattcaccca aggggtgccc aataactata
     2221 tagacgggct tatccggcaa accgtccgtt tccaaatctg gggaaaaaag tttgaggccg
     2281 aagaggtcat ccagaatacc cggctggccc tcttaagctg ttttagagaa gggaaataca
     2341 agggtaaagg gttgacaacc tacgtgtgcc gcatcgccgc catacaatcg gtaatggaga
     2401 tgagacgcca ctaccgctcc gaaaaatacc gggccgaatg gtccgaacag gccaaccagc
     2461 agccgggccc ggccggaaac cccttaacga cccttctgga acgcgaaaaa aaagatagag
     2521 cgatacgagt tctaaaaact ctgggcaaat tgtgccgtca actgctgatg ctcaaatttt
     2581 ataagggact gtcctacagg gaaataggcg ggcgcttggg cctaacggag gttaatgtca
     2641 gggtttccgt tcatcgctgc ctgcaaaaaa gcaaagagat cgccaagaaa atggaagaag
     2701 gccagtaaca ctttccctat ctgtgcaaca ataacaaagg tgctgtatgc cttgcgaaaa
     2761 tgaactaaag gaggccttgg gggattacct gaagggaatg ctccctgacc caaaaaccgc
     2821 aatggttcgg gagcatctga agcaatgccc ggactgccgg tcggacttgg accaactcca
     2881 attcctgctt cccggcctgg ccgggttgtc cgaagagcat tttccggtcg aaaccttggc
     2941 ggattacgcc ttccgtgtca aaacggctgc acggcaaacg gaaactttaa actggggaga
     3001 ggaaaaaatt gaagaacact tgcccgtttg cgaccggtgc caagccgaac tggataagct
     3061 gaccgctttc gaaaaagaat ttcagagggt ttccaaaaag agcgtaactg aaccttccct
     3121 tgaaataact cctttgagca ggtggtctct tttccgttct cgcaaagtct ggtatgccgc
     3181 ggccgccgcg gcggccttga ttctccttct tttacccact ctcccgctcc gctcccaggg
     3241 cccaatgata gcggtcttgc cgtttgaata tacaggcccg gccgaaaagg aatattatgt
     3301 ctttggagtc accgacgaga ttactgcccg gttaggcaaa gtgagcgcca tcggggtcat
     3361 cgctccgagc agcacgatgc aatacaaaaa cacggcccaa aaaccgcgcc aaatcggcga
     3421 ggaacttggg gtcctttatc ttttgaccgg ttctgttcgc tgggacacca ccgggaaaat
     3481 cgccaaactt caggtcgttc ccaaactggt gcgaaccaga gaccaaaagc agctctggac
     3541 ggaaccgttt gaaatcatta gaggggagct ctgcaatctc actggcgtaa ttgcccggaa
     3601 ggtgattgct gctttgaatg tcgaattttt ggaatcggag cgccaagcga tggaggcttg
     3661 tcccaccggc aacatcgaag cgtatgaata ttacctgcgc gggctggagt acgccaagcg
     3721 atatgagaaa gcgggcatag ccatacagat gtatgagaag gcggtggagc tggacccggg
     3781 ctttgctttg gcctatgccc agctttcgtt ttgtcacacc tttgtgtatt ggttcgggga
     3841 ccccaccgag gagcggcttt ccaaaataaa agagacgatg gaaagagcgt tccagcttca
     3901 gcccaacttg cctgaggccc acttggcaat ggcctggtat gtcagcatgg ccgacctgga
     3961 tattgagcgc atcctaaaag agtgcgccct tgctgtaaag gaacttccca acaatgtaga
     4021 cgccctctat atcaccggag ttatgcaaag gtgtgcgggc cgatatgaag tagcggttga
     4081 ctacttcaag aaggccgcca ggcttgaccc cctttctccc aacatcagtt tggagcttgc
     4141 ccatacctat ctctttcagc acaactatag agaggcagag cgcgaatatg gccggggcat
     4201 ttcggtcact ccagattacg cgaattttta tatctctgag gcatattttt tatgcctgta
     4261 cggggaaggc agcatcgaaa aggcgaggaa agtgctcgag aaggccgccg gaaaggttga
     4321 ctcgttacgg ctggctgcca tctgggctgt ggttgacctt tttgacggca attatcaaaa
     4381 ggcattgtcc cggctttcga gcgcgagttc gtttggtccg ataatacgcg gcagaacggc
     4441 gaccgattat tttctgatga aagcggagct ttatcgttta atgaaccagt ggcagttagc
     4501 gcacatttac tatgattccg cgcgaattga tttagaaccc aaagctatag gacacaaacg
     4561 gcctcgggat gcttttgtcc atcagaaact tggcgtcgcc tatgccggat taggccgcaa
     4621 ggaagatgcc attcgcgagg ggaaaaaggc cctggaactg cgaccaatga cgagagacgc
     4681 ccgttttggg gcaaatttag tcgaaggctt ggccgaaata tacgtgatgg tgggcgagta
     4741 tgatttggcc atagatcaat tggagtatct tttgtccgtt ccttctttga tttccgttcc
     4801 gtttcttcgg ctcgaccccg cctggattcc cctgcgcagc catcctcgtt tccaaaaact
     4861 cataaacgaa cgcgcttaaa aatcaaaaag ctctgtccgc atactgtatt ttgctttttt
     4921 gctttacccc aactgaaaaa actccgcaat cgccttccgg tgcgcggtga aggcaacgtc
     4981 ttcggggatg ttttcgggag caaaaaactc ggctttgact gcgtcatccc ccggctttaa
     5041 gcggccgctg acgatgcgcg ctaagtagat gatcaaaacc acctggctgc gcgggtcgtc
     5101 ctgcgccggg tagacgttga agagccggtc aatttcgata gtaaatccgg tttcctcttt
     5161 cacttcgcgg cgcacgcaag cggtgggaga ttcctcatac tcgataaatc ccgcggggag
     5221 cgaccacttc cccgcctgcg gctcaaactt gcgcttgacc atgagcaatt tgccgtcctg
     5281 gagtatgatc gcccccaccg ccggcaccgg attccggtag taaatgaaac cgcaggcggc
     5341 caccggacag accaaacgca tccgcccctc cagccgccgg cccactaatt cagctgaaca
     5401 cttggggcag aagacaaact gccccgccgc catctcccat tcgcccggct cccgctccgc
     5461 ctcccagtca atctttttca actcctcgcg gcggtaaagc ttctccgacg atttgacctt
     5521 ggtgaccggg tcgcgccccc actcctggcg gacctttggc cgagggattt tgattttctt
     5581 ttccatcttt tcctcaaaac accttaagca cgacgagcgt catgtcgtcc acctgcggcg
     5641 cgccggcgca aaagtcattg acggtcgtaa taattttatc cttcagctct tcggcgcaca
     5701 ggttccggtg ctcccgcacc aatttgcgga ggcgctcttc gccgaactgt tcctcctctt
     5761 gattctgggc gtcggtcaag ccgtcggtgt aaaaaatcaa actatcggcc ggacgcaagg
     5821 ccacccggcc cgcctcgtac cggctttcgg ggaaaacgcc cagaagcagg cctccggtgg
     5881 tgagctcctc ccagctgccg tccgcccgca gtaagatcgg gtggttgtgc ccggcgttgg
     5941 tataggtaaa gagtttcttt tccggctcaa aatcaccgca gaaaaaagaa acgaactttt
     6001 ccggtgaagt gctggaagcc actaactcat tgacgttgcc cactatttcc gccaatggct
     6061 tccggtgtcg gtactcggcc ttgagcgtcg cttggagcat cgcgaccaaa agcgccgccg
     6121 gcatcccctt gcccgaggcg tcggcgatca ccacgcccac tttgctctcg gccagaaggt
     6181 agtcgtagta gtcaccgccc acctggcgcg acggctggct gaaactggca aattcgaact
     6241 ccggccccct cggattgaat ctcggcaaaa gatcgaactg gatctgccgg gctaaagcca
     6301 gctcttcttc cagacgctgt ttttccagat gctcccggta cagttcggca ttggccaggg
     6361 ccacggtcaa ctggttggcc agaaccgaaa ggagggtgaa atcttcggcg gtatagcggt
     6421 agccggaatg cttgtcgctt aaagccaggc aaccgacgag gaatttctgc tccaaaagcg
     6481 gaacgatgac ccgcacccgc tctctctggc agaactggcc caggggtgac tcgcgcaggg
     6541 ccggttcggc cagttcggcc acgaagggct ttgatttccc gccaatatgg gcgatgaccg
     6601 gatcgtcctc ggcaaagagg accgtttctc tggcggaagt gggatcgcca gcgaacaaca
     6661 ggtacgcggg cttctcccgc tcccggagca agaggtacgc ccgctcaacc aacacctctt
     6721 tctccaatac ttccaggacg gtttgcttca attcacgcgg atcgaggatc gaaataattt
     6781 cacgagtgaa gcgctccatc aagttgcgga agtcgctggt cccgcggatg aaccagcgct
     6841 tgatcagatc gtcgatccgg ccgaagagcg gctggaagag gataatcgcc agcgcgatga
     6901 agccgacgtc caaaagcgga attttctgcc cgaagatgtt tttcaccagc cggccgaatt
     6961 gattggccga aaggacgtag agccccacca ccagagccga ggtgaccgag taggctaggc
     7021 tctggcggac caagagcttc acgtccatga actggtagcg gatgatgccc caggccaccg
     7081 caccggcgcc ggtgaccaga gccccgatgg tcaaaagctg ccggagcatc ggaccgggcg
     7141 acagggccgg cgccagtgcc gggagcaaaa aggccagagc gtacagaccc actccgccgc
     7201 ggatccccca gaaaatcagg cgcacctggg ctttcaaccg gggattggtc aaacggcggt
     7261 aaccgcgcca caaaagccag atcgacgccg ccacgtacac taagttcacc accgagaaga
     7321 gctggacgtg cgcctggtac aaaagcgaga ggaacgccaa aaggagtttg cccgccaccg
     7381 ccaaaggctc caaaattgcc cgcagcggag cagccatgtt ttcggtggaa aaccagcgct
     7441 ccagccgcgt cgggtcggag aagagactga ctaagagaac gtgaaagagg taggggaggt
     7501 agaacaccaa gcggagccgg gggaagcgcc gcacaaaggg gcgctcgtag ggaaaaacga
     7561 gcgcgaagaa caacagttgc ggaaagaaga gttcccagaa gtagaagagg ttgttgacca
     7621 aagccacccc gggccgcgcc aaaagttttt cgtccaccgg acccaggacg gtaccgaagg
     7681 cggcaaagag cgcccccagc gcggcgaaga agag
//
LOCUS       DBNGZA010000366         8039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1450334, whole genome shotgun sequence.
ACCESSION   DBNGZA010000366 DBNGZA010000000
VERSION     DBNGZA010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1450334"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC16FU_08045"
     CDS             complement(<1..319)
                     /locus_tag="AC16FU_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994356.1"
                     /translation="MPAETVTTRLSDEAIDKALVTAYRDLWGTFAKAVKAAVLYPPEN
                     PLPSQFRSQLTDKLTALLKAGDDLVFSFGPHLIRLGPQEVYSEPESKDSFVALFHRDG
                     VHQI"
     gene            complement(752..1429)
                     /locus_tag="AC16FU_08050"
     CDS             complement(752..1429)
                     /locus_tag="AC16FU_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR2994357.1"
                     /translation="MAQSKLSIHQATLDEPNQATPEVTTEEVLKILTSGIEPLLDVRS
                     AKEYAIAHIPGSINLYEKEAERIMQLYPDKAARLVLYCNGPYCGKSKRTTEQLVKLGY
                     TNVRRYQLGLPVWRALGHTVQTDLEGFRYVFMNDKTAVFVDARRPAQFADGSLPGAVS
                     IQAGEAEQANEDGRLPHTDKGTRVIVFANSPQEARTVAEEIAKKAYWNSSYFGGTIEE
                     LKRAGLW"
     gene            complement(1642..2976)
                     /locus_tag="AC16FU_08055"
     CDS             complement(1642..2976)
                     /locus_tag="AC16FU_08055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2994358.1"
                     /translation="MLTRERCQEICEKVFKFSNADETEVSFGGGESALSRFANNEIHQ
                     NVSEEGYYLSVRVVYGPKTGRASTNRYDDESIKQILKEASETAKLTPDDPELLPLVGP
                     QSYPPKNDFHKKTADFSPEERAKSVKPIFEQCKKEGLTAAGYLSNGSGVNAFATSKGC
                     FAYYTATDAGFSTTITSPDSTGWALDSSPNIAELDIGKDAGIAIQKAKASRNPKEVPP
                     GDYTVILEPAAVTDLLVFLMFGFSARQVMEKQSFLTDKVGTKLFGENITLLDDCYHPL
                     QSGQPFDGEGVPRQRLVLVEKGVVKNLCYDRLTAKKEGKEPTGHGFSVPNTFGAMPGN
                     LVMSGDSTTVDDMIKSIDKGILVTRFWYNRLVEPMKVIVTGMTRDGTFYIENGKIQYG
                     IKNMRFDESVVEMLNSVQALGKPVRASGVESPPMVVPPLKVAGFHFQSTTKF"
     gene            complement(3073..4527)
                     /locus_tag="AC16FU_08060"
     CDS             complement(3073..4527)
                     /locus_tag="AC16FU_08060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2994359.1"
                     /translation="MRDVLKLALDAASGSGVSYAEARIVQEKSQSITTKNGKVGSIRQ
                     DETFGLGIRVIAHGAWGFAATDDLSPKNVKRIAKKAVAIAKASALCKTHDIQLASEEK
                     HVSVWRAPFTKDPWAVPLSDKVDLLLKVDETCRKVKGVTAVEGNMNFVRETKYFLSSL
                     GSDIEQHRFRTGAGFSVSSFTDGELFERSYPASFGGQYSCKGYEMVDEINLLDNAQRI
                     AEEAVALHSAPQCESGVKDLILETSQLALQIHESIGHPIELDRVLGTEANYAGTSFLT
                     LDKLNKLQYGSKHMNVVADATLAHGQALGTFGFDDEGVPAQRNWIVKDGLFVGYMTSR
                     ETAPVVGASRSNGCMRADGWNRIPLIRMTNVSLEPGDGKFADLVADTKDGIYMSTVRS
                     WSIDDKRYNFQFGPEIAWEIKDGKLGRMLKNPSYGGITTEFWNSMDAVCGKPEWVLWS
                     VPNCGKGQPGQSMGTGHGCAPARFRNVKIGVAFG"
     gene            complement(4687..5745)
                     /locus_tag="AC16FU_08065"
     CDS             complement(4687..5745)
                     /locus_tag="AC16FU_08065"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR2994360.1"
                     /translation="MYNIAVVPGDGIGPEVINEALKVLKAVSELDKFDYRLQQYPYGS
                     EHYLSTGELVPGTWREELQKFDAILLGAIGDPRVEPGLVERAVIMTIRFGLDLYVNLR
                     PIKLYAEHLCPIKGKKPEDVDFIVVRENTEDLYAGIGGHLKKDTPDEVAIQEMIFTRK
                     GCERVVRYAFELARERNRQKKLTLVDKANAIRAMDIWTRTFEEVGQEYPDIIRDHAYV
                     DAACMWMIKNPEWFDVVVTNNIFGDIITDLGAMVQGGMGVAASGNIHPGKVSMFEPIH
                     GSAPKYKGKNVANPIAAIMAGAMLLDYVGEKKSAARVEKAVEHLLVTQRIKSLDAGSG
                     LSTTQIGDMVVAEIQKQK"
     gene            complement(5981..6733)
                     /locus_tag="AC16FU_08070"
     CDS             complement(5981..6733)
                     /locus_tag="AC16FU_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994361.1"
                     /translation="MKIKTKFWSAAVGSLPLMLGVYLPDSTVHSLKVAGGTGQYATIF
                     RDCAGNPTSSHGIPYKDVGGEYSFSPPGAASLGLRASRVWDRTVTGSFPDTFLLRRER
                     TIINPFFQLDWRKFGIGGGVAFNGRQNKVTGEKSAMPSWYLRIARGPIYFSSTFFSGV
                     PLYSGGGGLNMGIGGAARDFGWWAGWSAGFQDEETGLLLYDGNGFLGRADIRIAPGWL
                     VNVTGRAGASSGTFEGGVSLGLTKRWALPVQK"
     gene            complement(7429..>8039)
                     /locus_tag="AC16FU_08075"
     CDS             complement(7429..>8039)
                     /locus_tag="AC16FU_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994362.1"
                     /translation="SAFELVRRGYIVEPLILARHALEGAAVVVDILKSPQKLSKFFSQ
                     KYKSTGAISVAKEVIPIFGKIYGGLSDNLVHIGLLSCMPQWGMGPNFGIQSLFLGGGI
                     DENRIEDYVIVLCSLDLCTDVFNTIVEYSLFKFVDSPRFWRKKSETTLSFEPDDVIKD
                     RHEERQRKLVEAFQKLMGRVEKQQEILSRIDGGNDSNTLLPG"
BASE COUNT         1774 a         2228 c         2146 g         1891 t
ORIGIN      
        1 tgatctggtg caccccgtcc cggtgaaaga gggccacgaa gctatccttg ctttccggtt
       61 cggaatagac ctcctgcggt ccgaggcgga tcagatgggg cccgaaggaa aagactaaat
      121 cgtctcctgc cttgaggaga gcggtcaatt tgtcggtcag ctggctccgg aactgactgg
      181 ggagcgggtt ttcgggaggg tagaggaccg ccgctttcac cgccttggca aaggtacccc
      241 acagatcccg gtacgcggtg accaacgcct tgtcaattgc ctcgtcggaa aggcgggttg
      301 taaccgtttc agccggcata atgctcttct ttcagtcctt ttctgttatc ggcagaaatg
      361 acttgaagat agaccggctg attgaaccgg gggagcggct ctttcctttg ggaaaacccg
      421 ttatcgggga gaatggctca ggagtgggcc tctggggggt agagactaat aagctaaagc
      481 gtccctacag tcggaaatct gaaaagtgaa ctaccagatc tggattgatg ctccggggtt
      541 ggctgagggt tgggattgaa cagccccggc gccgtgagcc gtggttgcct aaaaagtcgg
      601 cgttcggcgt gatcagagag aaaaagcttt caccggaagc cactcccgcc aacgggagcg
      661 cccagagtgc gatgctgacc gcgctcagtt gcaaaaaatc acggcgggag agaaggaatt
      721 ctaccatcgg ttacttgctg cgtttttatg tttaccacaa acccgcacgt ttcaattcct
      781 caatcgttcc tccaaagtag ctgctgttcc aatatgcttt cttcgcgatt tcctcagcga
      841 ccgtgcgggc ctcttgtggg ctgttcgcaa aaacgatcac gcgcgtccct ttatccgtgt
      901 gggggaggcg accgtcttcg ttcgcttgtt ctgcctcgcc agcctgaatg ctaacggcac
      961 caggcagact gccgtccgcg aactgagcgg gacggcgcgc gtcgacgaaa accgcggtct
     1021 tatcattcat gaaaacatat cgaaaacctt ccaagtccgt ttgcacggtg tggccgagtg
     1081 cccgccagac cggaaggccc aattgatagc gcctcacgtt ggtgtaaccc agtttgacca
     1141 gttgctcggt ggttcgcttg ctctttccgc aatacggacc gttgcagtag agaaccaggc
     1201 gggctgcctt gtccgggtaa agctgcatga tacgttccgc ctctttctca tagaggttaa
     1261 tgctccccgg aatgtgcgca atggcgtact ccttcgcaga ccgcacgtca agcaagggtt
     1321 caatcccact ggtaagaatt ttcaggactt cctcggtcgt aacctccggt gttgcctggt
     1381 tgggctcatc aagtgtggct tgatggatac ttagcttcga ctgtgccacg gttgaattcg
     1441 atattcccag aaaacatgct accgctagtg cccacaccga caagactcgg gcgaaaagag
     1501 aatgtgtcat ttctgtcgct cctgcctttg aaaaacctga ttcatgagca cctggaccca
     1561 tacattacag cgttttggga aatgtggttt catctatgat ttttagaatc gagtcgtcaa
     1621 gacggaattg tccggtcagc attagaactt cgtcgtgctc tggaagtgga aaccggccac
     1681 tttcaaaggt ggcaccacca tcggcggcga ctccacgccg gaggcccgca ccggctttcc
     1741 cagggcttgc acgctgttca gcatctcgac cacgctttcg tcgaagcgca tattcttgat
     1801 tccgtactga atcttcccgt tctcgatgta aaacgtaccg tcccgggtca tcccggtgac
     1861 gattactttc atcggctcca ccagccggtt gtaccagaag cgggtgacga ggatgccctt
     1921 gtctattgat ttgatcatat cgtcaaccgt cgtactgtca cccgacatca ctaagttccc
     1981 cggcatagcg ccgaaggtat tgggtaccga aaaaccgtgc ccggttggct ccttcccctc
     2041 cttttttgcc gtcagccggt cgtagcagag gtttttcacc actccttttt cgaccagaac
     2101 gagccgctgg cgcgggaccc cttcgccgtc gaacggctgg cccgattgaa gcggatggta
     2161 acagtcgtca aggagagtga tgttctcgcc gaaaagtttg gtgccgactt tgtcggtgag
     2221 aaaggactgc ttctccatta cctgccgggc ggaaaaaccg aacatgagaa agaccagaag
     2281 atcagtgacg gcggccggct ctaaaatcac cgtgtagtcg ccgggcggca cttctttcgg
     2341 attgcggctg gccttggcct tctggatggc aatccccgca tccttcccga tatccagttc
     2401 cgcaatgtta ggagacgaat ccagcgccca gccggtggaa tcgggagagg taatggtcgt
     2461 tgaaaatccg gcgtccgtag cggtgtagta agcgaagcaa ccctttgagg tggcgaaggc
     2521 attgaccccg gaaccgttgg agaggtagcc ggcagcggtc aggccctctt ttttgcactg
     2581 ttcaaatatc ggtttaaccg acttcgctct ctcctccgga gaaaagtcgg cagttttctt
     2641 atggaagtcg tttttcggcg gatagctctg cgggccgacc aaaggcaaaa gttccggatc
     2701 atccggcgtc agtttggcgg tttcgctcgc ttctttcaaa atctgcttga tcgactcgtc
     2761 gtcgtagcgg ttggtagagg cgcggccggt tttcggcccg taaactaccc gtaccgagag
     2821 gtagtatcct tcttcggaaa cgttctggtg aatttcgtta ttggcaaagc gggataaggc
     2881 gctttccccg ccgccaaaag agacctcggt ctcgtcggcg tttgagaatt tgaaaacctt
     2941 ctcacagatt tcctggcaac gttcgcgggt tagcattctt cgccttatgt cggtagtccg
     3001 gctcgtctct gagccggacg gacgggctcg gggacgagcc cgtctaccaa aacggaccgg
     3061 tatccagatt tactatccaa acgccacgcc tatcttcaca ttcctgaacc gcgccggcgc
     3121 acagccgtgg ccggtgccca tcgactggcc gggctggcct ttgccgcagt tgggcacgct
     3181 ccagagcacc cattcgggtt tgccgcagac ggcgtccatt gagttccaga attcggtggt
     3241 gatgccgccg taggaaggat tcttgagcat ccgccctaat ttgccgtctt tgatctccca
     3301 ggcaatctcc ggcccgaact ggaagttgta gcgcttgtcg tctatcgacc aggagcggac
     3361 cgtggacatg tagataccgt ctttggtgtc ggcgacgagg tcggcgaact tcccatcgcc
     3421 cggttctaag gaaacattgg tcatgcgaat caagggaatc cggttccagc cgtcggcgcg
     3481 catgcagccg ttggagcggg aggcgcccac caccggcgcg gtctcccggg aggtcatata
     3541 gccaacgaaa aggccgtcct tgacgatcca gttgcgctgg gcgggcaccc cctcgtcgtc
     3601 aaaaccaaag gtgcctaacg cttgaccgtg cgccagcgtc gcgtcggcca ccacgttcat
     3661 atgcttcgag ccgtactgca acttgttcag tttgtccagg gtcaaaaacg aggtcccggc
     3721 gtagttcgcc tcggtgccca ggacgcggtc caactctatc ggatggccga tcgactcgtg
     3781 aatttgcaaa gctaattgcg aagtttccag aatcaagtct ttgacgcccg actcgcactg
     3841 cggcgcggag tgcagggcca ccgcttcttc ggcaatgcgc tgggcgttgt cgagcaggtt
     3901 gatttcatcc accatctcgt accctttgca ggagtactgg ccgccgaaag aagccgggta
     3961 ggagcgctcg aacagttcgc cgtcggtgaa ggaggagacc gaaaagcccg cgccggtgcg
     4021 gaaacggtgc tgctcgatat cggagcccag agaggagaga aagtactttg tttcccggac
     4081 gaagttcata ttcccttcga ctgcggtgac tcccttcacc ttgcggcagg tttcatccac
     4141 tttgagcaaa agatcgactt tgtcggagag cggcacggcc caggggtctt tggtgaaggg
     4201 cgcccgccag acggaaacgt gtttttcctc cgacgccagt tggatgtcgt gagttttgca
     4261 cagggctgaa gctttagcga tggcgaccgc ctttttagcg atccgcttga cgtttttggg
     4321 ggaaaggtcg tcagtagcgg caaaccccca ggcgccgtgg gcgatcaccc gaatgcccag
     4381 gccgaacgtt tcatcctgcc ggatggagcc gaccttgccg ttcttggtgg tgattgactg
     4441 ggatttttcc tggacgatcc gggcttcggc gtacgacacg ccggaacccg acgcggcatc
     4501 cagcgccagt ttcaaaacat cacgcaaaat ttaacctccg catcagccat tcactccgga
     4561 tgaccggcta atatagccag acaaacgctg cgcgccagca gaatttttga aagaaaactc
     4621 cctattgtga aagaaatcac aaaagagaag gttttgattg accggcaaac ttcgggcggg
     4681 gcgtttctat ttttgttttt gaatttcggc aacgaccata tcgccgattt gtgtggtcga
     4741 gagaccggag ccagcgtcga gcgatttgat tctttgcgtg accaaaagat gttcgaccgc
     4801 tttctcaact cgcgcggctg atttcttttc gccgacgtaa tccaaaagca tcgccccggc
     4861 cataatcgcc gcaatcgggt tggcgacgtt tttgcctttg tacttcggcg cagagccgtg
     4921 aatcggctcg aacatcgata ccttgcccgg gtggatgttc ccggaagcgg ccacccccat
     4981 cccgccctga accatcgcgc ctaaatcggt gatgatgtcg ccgaagatgt tgttcgtcac
     5041 gaccacgtcg aaccactccg ggtttttgat catccacatg caggcggcat cgacataggc
     5101 gtggtcgcgg atgatgtcgg ggtattcctg cccgacctct tcgaacgtcc gggtccagat
     5161 atccatcgcc cggatggcgt tggctttgtc cacgagcgtc aattttttct ggcgattgcg
     5221 ctcgcgggcc agttcaaaag cgtagcggac tacccgctcg caccctttgc gggtgaaaat
     5281 catttcctgg atcgccacct cgtcgggggt gtctttcttc aagtgcccgc cgatcccggc
     5341 gtacaggtct tcggtatttt cgcgcaccac gatgaaatcg acatcctccg gctttttgcc
     5401 cttaatcggg cagagatgtt cggcgtagag tttgatcggg cgcaggttga cgtagaggtc
     5461 cagcccgaag cggatggtca taatcaccgc ccgctctacc aaccccggct ccacccgcgg
     5521 atcaccgatg gcgcctaaga ggatcgcgtc gaacttttgg agctcctccc gccaggtgcc
     5581 ggggactaac tcgcctgtgg agaggtagtg ctcgctgccg taggggtact gctggaggcg
     5641 gtagtcgaac ttgtctaatt cggagacagc cttgaggact tttagcgctt catttatgac
     5701 ttccggcccg ataccatccc cggggacaac agctatgttg tacatgggaa gcgaagatag
     5761 aaagaattgg ggaggattca aatacgaatt atcaccacgg aggcgcttcc tttcccgcgt
     5821 aggccggact tttagtcctg cagcacagcg cgtagccgag gcgccccgcc tcggacaaag
     5881 gcggcactga aagtgccgcc tacgcggaga gcggtcgctc ctttccgcgt atgccggtta
     5941 aagactcgta ccgaggtttc agcccggccg gagcagattt tcacttctgg acgggtaggg
     6001 cccagcgctt ggttaaaccc aaactgacac cgccctcgaa cgtacccgaa cttgccccgg
     6061 cccgtccggt cacgttcacc agccaccccg gcgctatcct tatatcggct cggccgagga
     6121 agccgttgcc gtcataaagc aaaagcccgg tttcctcgtc ctggaaaccc gcgctccaac
     6181 cggcccacca gccgaaatca cgtgccgctc cgccaatccc catattgaga ccgcccccgc
     6241 ccgagtaaag cggaacaccg ctgaagaagg tcgaagaaaa atagatcggc cctctggcta
     6301 tccgtagata ccacgaaggc atggcgctct tttcaccggt cactttattc tggcgcccgt
     6361 tgaaggcgac accgccgccg atcccgaatt tccgccaatc cagctggaag aatgggttga
     6421 taattgttct ctcacggcgc aagagaaaag tatcgggaaa actccctgtc accgtgcgat
     6481 cccaaacgcg gcttgcccgg agccccaagc tggctgcgcc gggtggagaa aacgagtact
     6541 cgccgccgac gtctttgtac gggatcccgt gactactcgt cgggtttccc gcacagtcgc
     6601 gaaagatcgt ggcgtactgt ccggtgcccc cggcaacttt gagggaatgg acggtgctgt
     6661 cgggaaggta aacgcctaac atcaatggga gtgatcccac ggctgctgac caaaatttag
     6721 ttttgatttt cattgcgacg aacctcctct atggccgagg cttcgctact gttctgaccc
     6781 gcttccggcc cgctgccatc cccggagaca acggcgatct tgtgcatgca gagcgaagat
     6841 agagagaatt gcgggaggat tcaaatacaa aaatgcacga cggaggcacg gagtgaaaag
     6901 agagaaattc tttttaggtc cataacctgg atccccgagg ccccttacct cggattactc
     6961 cgaggagatc gcttcaaaat ccaatatcca aaccacgcaa gaatagtaat aattaaaacg
     7021 tggataatta ctacattcca cacaaacttc gctggacgag gtggcgtgtt gaacaaaaag
     7081 tgaaacggtc ggctcgaata ggtagccata aaacgcccac gtttattaac taactcttga
     7141 tgagcattaa taaggtcttg catgggaggg gttatttcac ctccgctaag ttccgtcata
     7201 cctttaatta gatcgtcaaa gttcgccatc agttctttgg ttcgtttttc ttcttctact
     7261 cgatctgcta gggagatttc tacaggagga aataggaatc gtaacattat cagaatgaag
     7321 ccaaaaataa ggataaggcg ttgtcttagt ttcacttggt tacctctccc caatttccaa
     7381 agtcatatta agtagtcctt cgatactttt cagaaaaggg ctggactttt aacccggcaa
     7441 gagggtgtta gaatcgtttc caccatctat tcttgacaat atctcctgtt gcttttcgac
     7501 tcgacccatt aatttttgaa atgcttctac caactttctt tgtctttctt catggcgatc
     7561 ttttattaca tcgtcaggct cgaaagaaag agtggtctca ctcttttttc tccagaatct
     7621 gggtgaatca acaaacttaa atagcgaata ttcaactatc gtgttaaaaa catctgtaca
     7681 aagatccaac gaacacaaaa cgattacata atcctctatc ctattctcat caatcccacc
     7741 tcccaaaaaa agtgactgaa tcccaaagtt tggccccatc ccccactgag gcatacaaga
     7801 aagaagtcca atatggacaa gattatctga caggccacca taaattttcc caaaaatcgg
     7861 aataacttct tttgcgacgc tgattgcacc agttgatttg tatttttggg aaaagaactt
     7921 tgaaagtttc tgcggggatt ttagaatgtc cacaactacg gcagctcctt ccagcgcgtg
     7981 tcgtgccaat attagaggct cgacaatata tcctcggcgt actaattcaa atgcactaa
//
LOCUS       DBNGZA010000367         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_71992, whole genome shotgun sequence.
ACCESSION   DBNGZA010000367 DBNGZA010000000
VERSION     DBNGZA010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_71992"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1576
                     /locus_tag="AC16FU_08080"
     CDS             <1..>1576
                     /locus_tag="AC16FU_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2994363.1"
                     /translation="GDDLVFSFGPHLIRLGPQEVYSEPESKDSFVALFHRDGVHQISF
                     SPGVEAAETAGLIDAVAKALSSHGDGDDLVTLLWQKEFLHIKYQVVEDVAEVAAVELN
                     LGKGSKAESDPLVYVNLVLQEGKAGTGEGSGEGDKAGEGQAAAPPAHAAAVRKVFGDI
                     EQMAQEDRAAIARELSENKVFPWSKEALELLCEMVYLENEPAGVAETVGILEKILDEL
                     IAGGRVQPTAQIVTSLRQLLEDFRSKPPEWARPVQAALDKAGDPIRLSRMTELLNANK
                     VDDPEAVAHYLRTLDWKSVGNIHALLGTLEHYPHRKLVCQVLEQMGPPAVELIAKGMA
                     DPRWFVVRNTALILGHIGGDKACQHLRAGLSHSDVRVRRETLLALEQIGTPTASKMVL
                     GVLNEKDGKLRSKALQILVHLQEAEACEPLWLRIESKDFLQWSPEEQRELVFAYAQIG
                     RDRVLEELAGLVTRQAFFKRGLYQPIKEFAVEAMAKVGTLRAQEYLERLAGTTHGALK
                     TACQTWAQRLKRRQVQG"
BASE COUNT          330 a          438 c          522 g          286 t
ORIGIN      
        1 ggagacgatt tagtcttttc cttcgggccc catctgatcc gcctcggacc gcaggaggtc
       61 tattccgaac cggaaagcaa ggatagcttc gtggccctct ttcaccggga cggggtgcac
      121 cagatcagtt tttctccggg agtggaggcg gctgagacgg ccggtttgat tgacgccgtc
      181 gccaaagccc tttcctccca cggcgacggt gacgacttgg tcacgctgct ctggcagaaa
      241 gaatttctcc acatcaagta ccaggtagtt gaagacgtgg ccgaagtggc ggcggtggaa
      301 ttgaacctcg gcaaagggag caaagccgag tccgatcctt tggtgtacgt gaacctggtg
      361 ctgcaggaag ggaaagccgg caccggagag ggttcgggtg aaggggacaa agcgggcgaa
      421 ggtcaggcag cggcgccgcc ggcccacgcc gccgcagtgc gcaaagtctt cggcgacatt
      481 gaacagatgg cccaggaaga ccgcgccgcc attgcccggg aactgtcgga gaacaaagtt
      541 tttccctggt ccaaagaggc gctggaactt ttatgcgaaa tggtctacct cgaaaatgaa
      601 ccggccggcg tggccgagac ggtgggtatc ttggagaaaa tcttagacga attgatcgcc
      661 gggggcagag ttcaacccac ggcgcagata gtcacttccc tgcgccagct gctggaggac
      721 tttcgctcca aaccgccgga atgggcgcgg cccgttcagg cggccctgga taaggccggc
      781 gacccgattc gtctttcgcg gatgactgag ctgctcaacg ccaataaggt agacgatccg
      841 gaagcggtgg cccattacct gcgcacctta gactggaaat cggtcgggaa catccacgcg
      901 cttttgggga ccctggagca ctacccgcac cggaaactgg tctgccaggt gttggagcaa
      961 atgggcccgc cagcggtgga gttgatcgcc aaggggatgg ccgatccgcg ctggttcgtc
     1021 gtccggaata cggccttgat tctggggcac atcgggggtg acaaggcttg ccagcacctg
     1081 cgggccgggc tcagtcactc ggacgtacgg gtacggcggg agacgctttt ggccctggag
     1141 cagatcggca cgccgaccgc gagcaaaatg gtcttaggcg tactcaacga gaaggatgga
     1201 aaactgcgga gcaaggcgtt gcagattctg gttcacctgc aggaagccga agcctgtgaa
     1261 ccgctctggc tgcgcattga gtcaaaagat tttctgcagt ggtctccgga ggagcaacgc
     1321 gaactggttt ttgcctacgc ccaaatcggc cgcgaccggg ttctggaaga actggccgga
     1381 ctcgttaccc ggcaggcgtt tttcaagcgc ggactgtacc agccgatcaa agagttcgcc
     1441 gtggaggcaa tggcgaaggt gggtaccctg cgggcccagg agtacctgga gcgtctggcc
     1501 gggacgaccc atggcgcgct gaaaaccgcc tgtcagacct gggctcagcg tctgaaacgc
     1561 cgccaggtcc agggag
//
LOCUS       DBNGZA010000368         1228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1236191, whole genome shotgun sequence.
ACCESSION   DBNGZA010000368 DBNGZA010000000
VERSION     DBNGZA010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1236191"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1129)
                     /locus_tag="AC16FU_08085"
     CDS             complement(155..1129)
                     /locus_tag="AC16FU_08085"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR2994364.1"
                     /translation="MIASTSPGAVLHGKEAHPISEAVLTTEIIDYPLYARGKVRDIYD
                     LGDRLLFVASDRISAFDVILRTGIPGKGKILTGLSVFWFDFLKEIVPNHFITADVRDY
                     PKPLKKYESILAGRSMLVKKVKRIDLECVVRGYIAGSFWKEYVEARKNSGAGNIILHG
                     EVLPNNLKESEILPFPIFTPATKADTGHDENISLAEAQKIVDSETVLKLRETSLRLYQ
                     KAADYAQERGIIIADTKFEFGRLGGQLILIDEVLTPDSSRFWPAEKYGPGRPQESFDK
                     QYVRDYLEEVQWNKKPPAPPLPPEVVRNTRKKYLDAYQKLTGQNPPPE"
BASE COUNT          255 a          341 c          362 g          270 t
ORIGIN      
        1 atgccggccg cacgcaggtg ggcgagcgtg ccgccggtcg aaagaatttc gacgttaaaa
       61 gaaatcagga cgcgtgccaa ctcctgtaga ccggacttgt ccgaaacgct caccagcgcc
      121 cggcggatcg gcaccaaatc gccagtgacg agcattactc cggtggcgga ttttgcccgg
      181 tcaatttctg gtacgcgtcg agatatttct ttcgtgtatt ccgtaccacc tccggcggga
      241 ggggcggggc gggcggtttt ttgttccatt ggacctcttc caagtagtcg cgcacgtact
      301 gcttgtcgaa actctcctgc ggacgtcccg gtccgtactt ctcggccggc cagaagcggg
      361 aggaatcggg ggtgagcact tcgtcaatta gaatcaactg accacccagg cggccaaact
      421 cgaatttcgt gtcggcgatg atgataccgc gctcctgcgc gtagtcggcg gccttctgat
      481 acagccgcag gctggtttcg cgcaacttga gtacggtttc agaatcgacg atcttctggg
      541 cctcggccag tgagatattc tcgtcgtggc cggtgtccgc tttcgtggcg ggggtgaaaa
      601 tcggaaaagg caagatctcc gattccttca gattgttcgg cagcacctcg ccgtgcagga
      661 tgatattgcc cgccccagaa tttttccggg cctccacgta ctccttccag aacgaaccgg
      721 cgatgtagcc gcgcaccacg cactctaagt cgatccgctt cacctttttg accagcatcg
      781 aacggccggc caggatagac tcgtactttt tgagcggctt ggggtagtcc cgcacgtcgg
      841 cggtgatgaa gtggttcggg acgatctctt tcaagaaatc gaaccaaaag accgaaagcc
      901 cggtgaggat cttccccttg cccggaatgc cggtgcgaag gatgacatca aaggcggaga
      961 tgcggtcgct ggcgacgaag aggagccggt cgcccagatc gtagatgtcc cgcaccttgc
     1021 cgcgggcata gagcggataa tcaatgattt ccgtggtaag gactgcttcg ctgatcgggt
     1081 gtgcctcttt cccatgcaga actgctcccg gcgatgtgct tgcgatcata tcggctctcc
     1141 gattgtcacg ggttttaatc taaagataat acagatggag gcaaatggaa aatggagcga
     1201 acggttctgc cgacaaaaat ccgtgagc
//
LOCUS       DBNGZA010000369         7205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1025026, whole genome shotgun sequence.
ACCESSION   DBNGZA010000369 DBNGZA010000000
VERSION     DBNGZA010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7205
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1025026"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16FU_08090"
     CDS             complement(<1..880)
                     /locus_tag="AC16FU_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR2994365.1"
                     /translation="MPGEFQNDTLKRFSHLDWLIGALVFLASFFVFYLTKAPSVSFWD
                     CGEFVACAAILGIPHPPGSPLMVVLGRVFTLLPLNPDIAVRVNLISVVTSAGCAFIGY
                     RIVRRLVRQWFTPSAAEGWSSELWAQVGGVSGGLLLAFGSTFWSSAVEAEVYGLAMFL
                     FLYISDLALQWLWETDGAKRQRLLALMIYLAVLSTAVHMTTFLVIPALFLLLVIADPK
                     LRSDWRVWVTFGVIGLIVMAVGPFLIALGVWLVVSLLARLAAGQTGRWRLPLVLALMA
                     VLGFSSQLFIPIRAAQK"
     gene            1010..1300
                     /locus_tag="AC16FU_08095"
     CDS             1010..1300
                     /locus_tag="AC16FU_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994366.1"
                     /translation="MLAWCAIMFCLGVLAFMDSLFNYGEIFRQVNSVLFMLVSLGLLV
                     RTTTKIRARTVEGYIEKVSRLEEEIKQISATRKSASGGHDVRSERFVPSGSR"
     gene            complement(1303..3162)
                     /locus_tag="AC16FU_08100"
     CDS             complement(1303..3162)
                     /locus_tag="AC16FU_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994367.1"
                     /translation="MLSRKTPRALLAGCVIVILCASAAPAQVSPDFQQNLNRVDQLLL
                     LNKFDEAVTLAEQLQKRYGDSSELLSRLKQAYLGAKRYADLITLLDREVARNPQDQET
                     LAQLASIYLKAGEKPKAEQTVKKLIALAPAEASVYSRAAGVYTENGYAREAIDWYGAG
                     RKALGNPRAFSWEVASLFEALRDYPAALREYFTQIDLEPTDAGLEARVTQLVNQVEAD
                     SSIERTLAEFTRWPGRPARGGEVPQPAGRVLAYRLYGDMLYNRGEIRLAVEEYRSADQ
                     YGGTRGTNLLRLILKLLADGYYAVADSACQALIAQIPNDPLVMQALLLQAQARTGEKR
                     YASAGDIYRQVVETTPLPREKAEALLAIALLHLEALNQPAVAQAEFQSLVTNYPDLSL
                     AQEARLRLGDCLLAQDKLDEALRYFQDFSRETNSAILGERLALRLAELFFYRGQVDSA
                     ADAYRLIVERYPRSTAVNDALNRLALLAFSGLAASPELSLFSRAEKLRTQRQFEKAIA
                     LYEKLADAGWQIDQVLWAQALALRAAGEYPKALVALAKLKTERLTSRWAACALKLSGE
                     IRLEDLGDPAGARAAFEEILKNYPDAIFTEEVRTRLKRLPPGSGGAESLRGGS"
     gene            complement(3171..6530)
                     /locus_tag="AC16FU_08105"
     CDS             complement(3171..6530)
                     /locus_tag="AC16FU_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994368.1"
                     /translation="MANESFQKIVERLVGLDRARKLLTTQWVLASIITLLVGAFLTIL
                     VFSFLFTLPASIRIAVWLILGGTFIYLTGLRWLPMFLRRANLYRLARLVEAHFPDLEN
                     RLVAALDCQRFLQEHQPNYSPELMAAVIVAADRKTENLDFARVLDRQPLKKALRLAGL
                     TLAATFLLSLVSPANFRASFRAFLHPTEELKQSPPFQVLVSPGSVEVVKFTPVTLKAT
                     AIGQNLPRRLFVNWQNEGGQWQKELLSREALSPTANGIDTAVFTYAIREAKKNFTYFF
                     SGKNFQSEAYAITVVDIPRVTDIRATLTYPAYSRLGAVTKTDNDGNLQALTGTRVTLS
                     VETNKPVDKATLVFGDGTSKQMNVRGKTAAVPFPLTRSTTYHIQVVDHVGNANRDPIE
                     YTLTALPDAPPSVEIVRPGSDLDLSEEMQVGLEVKAQDDYGFSTMLLKYQIVSHGISG
                     AERKKELSGASVGTELIIRDRWDLANLPLVPGDLVRYWVEVADNDNVSGPKWAKSKTY
                     HLRLPSLDEILSEATQKTDENISDLEKVLSGEKDLNRKLEELSRRLAKDQKLSWETKE
                     EVQKLAAEHLKFLENLNKVADQQHETAKKMAENRLAQQELVEKMQEMQKLLDEVASPE
                     LKAALRKLQQALDQLDPEQVRQALEKFQIDQEALIAKLDRTIELLKRLATEQKLDALL
                     KMAEKLLQGQKEVNAKTQASPADSLPQVSPDEKALEKQLDQLTQKASDLKRELQADPE
                     FNIPQSAQFCDRPGQSGAKPKMQKMQQALQQAQKKPSEQAGAEAQEEMESMFASMLQA
                     RQGISQCRKKGILGELRKALEDALYLSESQEKLAADLNTSDASGRKLRQFAQEQNDLK
                     TETERLSQRLEGTAKKSFFVPQPKNSPTYQAVARMQQAVTHLSNCSRAGAQQEQTEAL
                     YQLNQTAKELLSAIQNAAASSCGSGGEELLQKMQNLSNLQLGINEQGMSLLPPNQPAQ
                     DQLASLQRLRAEQEVVKKSMEELQREYGSRKEILGRLDELGKEVEKVLEDFDRQNYGS
                     QTLDRQKKILSRLLEYNQSLERRDYTERRQAEFGEDIIRRSPQLSREQIEKAQRQSDT
                     AFPFGDEPYPPEYEQVIKNYLKAYGGEAPRK"
     gene            complement(6537..>7205)
                     /locus_tag="AC16FU_08110"
     CDS             complement(6537..>7205)
                     /locus_tag="AC16FU_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR2994369.1"
                     /translation="VHSQPVRVTSLTDPQTFTVARLKYGGGGDWYWGPSALPNLLRYI
                     KENTSIPVNENEVRVSLADDDLFNYPFLYMTGHGNIFFTEAEVNRLRTYLTSGGFLFA
                     NDSYGLDKAFRREIKKIFPEADLVELPFDYGLYHSYFDFPNGLPKIHKHDGKPAQGFG
                     ILHQGRLVVFYAYESDIGDGWEDPQVHKDPPEKREQALKMGTNLAVWALLGGKEPSKV
                     SASK"
BASE COUNT         1397 a         2088 c         2200 g         1520 t
ORIGIN      
        1 gcttctgcgc cgcccggatc ggaatgaaaa gctgcgagga aaaccccaag accgccatca
       61 gcgctaacac caaaggcagc cgccagcggc cagtctgtcc cgcggccagc cgcgccagaa
      121 gactcaccac cagccagact cccagggcaa tcagaaacgg ccccaccgcc atcacgatca
      181 gcccgatcac cccgaaggtg acccagaccc gccagtcgct gcgcagcttc ggatcggcga
      241 tgaccaaaag caggaacaaa gccggtatca cgaggaaggt ggtcatgtgc acggcagtcg
      301 agagcacggc taaatagatc atcagcgcca aaagacgctg ccttttcgcc ccgtcggtct
      361 cccacaacca ctgcagggcc aggtccgaga tatacaagaa cagaaacatc gccagcccgt
      421 acacctccgc ctccaccgca gaagaccaga aggtgctccc gaaggccaaa agcaagcccc
      481 cggaaacgcc ccccacctgc gcccacaact ctgaactcca cccctcggct gcgctcgggg
      541 taaaccactg ccgcaccagc cgccgcacga tccggtagcc gataaacgcg caccccgccg
      601 aggtcacaac cgagatgaga ttcacccgca ccgcaatgtc cggattgaga ggcaaaagcg
      661 tgaacacccg ccccaaaacc accatcaaag gcgaccctgg cgggtgcgga atccccaaga
      721 tcgccgcaca ggccacaaac tccccgcagt cccagaacga cacgctcggc gccttcgtca
      781 ggtaaaagac gaagaaactg gccaggaaaa ccaaagcgcc gatcagccag tcaaggtgcg
      841 aaaatctctt cagtgtatcg ttctgaaatt cacccggcat actcttcgtc gcttaaggga
      901 gcgtcaaatt tgccgaaaac aaaaacaggg caatggtacg acccggggtg caccttgtca
      961 aggcagggca ccagcggacg gccggctcga acgtgagaag gaggaaagca tgttagcgtg
     1021 gtgtgccatt atgttctgtc tgggagtgct cgccttcatg gacagtctct tcaactacgg
     1081 cgaaatcttc cgccaggtga attcggtgct gttcatgctg gtttctctgg gtcttttggt
     1141 ccggaccacc acgaaaatcc gggcgcgaac cgtggaaggg tacattgaga aggtaagccg
     1201 cctggaagaa gaaatcaagc agatctccgc cacccggaag tccgcctcag gcggacacga
     1261 tgtccgctcg gagcggttcg ttcccagcgg cagccggtag ttttacgaac caccgcgcaa
     1321 actttcggcc ccgccggagc cgggcggcag gcgtttcagc cgggtgcgca cctcctcggt
     1381 gaaaatggcg tcggggtagt ttttcaggat ttcctcaaag gcggcccgcg cgccggccgg
     1441 gtcgcccaga tcctccagtc gtatttcacc cgacaatttg agagcgcagg ccgcccagcg
     1501 gctggtgagc ctttcagttt ttaatttcgc cagcgccacc agcgccttcg ggtactctcc
     1561 cgcggcacgc agcgccaggg cctgggccca gagcacctgg tcgatctgcc agccggcgtc
     1621 ggctaatttt tcgtacagcg caatcgcttt ttcaaactgc ctctgggtgc ggagtttttc
     1681 ggcccggctg aagagagaga gttccgggct ggccgccagg ccggagaacg ccaaaagcgc
     1741 cagccggttc aaggcgtcgt tgacggcggt ggagcgcggg tagcgttcga caatcaggcg
     1801 gtaggcgtcc gcagcgctgt cgacctgacc ccggtagaag aaaagctcgg ctaggcgcag
     1861 cgcgagccgt tcgccgagga tggccgaatt ggtctcgcgg gaaaagtctt gaaagtagcg
     1921 cagggcctca tccagtttat cctgggcgag gaggcaatcg cccagacgca gccgggcttc
     1981 ctgggctaaa gaaaggtccg gatagttggt cacgagtgat tgaaactccg cttgagcgac
     2041 ggcgggctgg ttcagcgctt cgaggtgcaa cagcgcaatg gccaggagtg cttcggcttt
     2101 ttcgcgcggg aggggggtcg tctccaccac ctggcggtag atatcgccgg cgctcgcgta
     2161 gcgtttctcg cccgtccggg cctgagcttg caaaaggagc gcctgcatga ccaaggggtc
     2221 gttcggaatc tgggcgatca aggcctggca ggccgaatca gcgacagcgt agtaaccgtc
     2281 ggccaaaagt ttcaaaatga gccgcaggag attggttccc cgggtgcccc cgtactggtc
     2341 ggctgagcga tactcttcta cggccagccg gatctccccg cggttgtaga gcatgtcccc
     2401 gtacagccgg taggccagga cccggccggc cggctgcggg acttccccgc ctctggcggg
     2461 gcgacccggc cagcgggtaa actcggctaa ggtccgctcg atactggagt cggcttcgac
     2521 ctggttgacc aactgcgtta cgcgcgcctc cagaccggca tcggtcggtt ccagatcgat
     2581 ctgggtgaag tactcccgca gcgcggccgg gtagtcgcgc agcgcttcaa aaagcgaagc
     2641 cacttcccag gagaaggcgc gcggattgcc cagcgccttg cggccggcgc cgtaccagtc
     2701 gatcgcctcc cgggcgtagc cattttcggt atatacccca gcggcgcggc tgtagaccga
     2761 ggcctctgcg ggggcaaggg cgatgagttt tttgacggtt tgctcggctt tgggtttctc
     2821 gcccgctttc aggtagatgc tcgcgagctg ggccagcgtt tcctggtctt ggggattgcg
     2881 tgcgacttcc cgatccaaaa gggttatcag gtcggcgtac cgttttgctc cgagataggc
     2941 ctgtttgaga cgggagagga gctcactgga gtcgccgtag cgcttttgca actgttctgc
     3001 aagcgtgacg gcctcgtcaa acttgttcaa cagcaatagc tggtctaccc ggttgaggtt
     3061 ctgctggaag tcgggcgaaa cctgagcagg cgcggccgaa gcgcacagga tcacgatcac
     3121 acacccggcc aaaagcgcac ggggagtttt tcgggaaagc ataggttggg ctacttccgg
     3181 ggtgcctcgc cgccgtaggc cttgaggtag tttttgatca cctgctcgta ttcgggcggg
     3241 taaggctcat ccccgaacgg gaaggcggta tcgctctgcc gttgggcctt ttctatttgt
     3301 tcgcgggaaa gctgggggct gcggcggatg atgtcttcac cgaattcggc ctggcggcgc
     3361 tcggtatagt cgcgccgctc cagcgactgg ttgtactcca ggagacgcga aagaattttc
     3421 ttctgacgat ccagagtctg agaaccgtag ttctgacggt cgaagtcttc cagcaccttt
     3481 tcgacttcct tgcccagttc atccagccgg cctaagattt ctttgcggct gccgtactcc
     3541 cgctggagtt cttccatact cttcttcacc acctcctgct cggcgcgcag gcgctgcagc
     3601 gaggccaact ggtcctgggc gggctggttg ggagggagaa gggacatccc ctgctcgtta
     3661 atccccagct gcagattgga caggttctgc atcttctgca gcagttcctc gccgccggag
     3721 ccgcaggatg aggcggcagc attctggatg gcggagagca gctctttggc ggtctggttc
     3781 aactggtaga gggcttcggt ttgttcctgt tgggcgccgg cccgggagca attggagagg
     3841 tgcgtgaccg cctgctgcat ccgggccacc gcctgatagg tgggggaatt tttcggctgc
     3901 ggcacgaaga aactcttttt ggcggtgcct tccaggcgct gcgagagacg ttcagtttcg
     3961 gttttgaggt cgttctgctc ttgcgcaaac tggcgcagct tccggcccga ggcgtcggag
     4021 gtgttcaagt cggcagccag cttttcttgg ctttcggaca ggtagagggc atcctccagc
     4081 gctttcctca gttcgcccag aatgcctttc ttgcggcact gggaaattcc ctgccgcgcc
     4141 tggagcatgg aggcaaacat cgattccatc tcttcttgcg cttcggcacc cgcctgttcc
     4201 gaaggcttct tctgcgcctg ctggagcgcc tgctgcatct tctgcatctt cggtttggcg
     4261 cccgactggc cgggccggtc gcagaactgg gccgactgcg gaatgttgaa ctcggggtcc
     4321 gcctgcagtt cccgcttcag atcggaggcc ttctgggtca actgatccaa ctgtttttcc
     4381 agcgcttttt catcggggga aacctggggc agactgtcgg ccgggctggc ctgggttttg
     4441 gcgttgacct ctttttggcc ctggagaagt ttttcggcca ttttcaagag cgcgtccagc
     4501 ttctgctcgg tagccaggcg tttcaacagt tcaatcgtgc ggtcgagttt ggcgatcagg
     4561 gcctcctggt cgatttgaaa cttttccaac gcctggcgga cctgttcggg gtcaagctga
     4621 tccaaggcct gttgcaattt gcgcagggcg gctttcagtt ccgggctcgc cacttcgtcc
     4681 aagagtttct gcatttcctg cattttttcg accagttctt gctgggccag gcggttttcg
     4741 gccatttttt tggccgtctc gtgctgctgg tcggccacct tgttcagatt ttccaaaaac
     4801 ttgaggtgtt cggcggctaa tttctgcact tcctctttgg tctcccagga gagtttctgg
     4861 tctttggcca ggcggcggga gagttcctcc agtttccggt tcagatcctt ctccccggag
     4921 agcacttttt ccaggtcgga gatgttttcg tcggtcttct gagtggcttc acttaagatt
     4981 tcgtccaaag acggcagccg caggtggtag gttttgcttt tggcccattt ggggccggag
     5041 acgttgtcgt tgtcggccac ctccacccag taccgcacca ggtcgcccgg aacaagaggc
     5101 aaattcgcca aatcccagcg gtcccggatg atcaactcgg tcccgacaga agcgccggag
     5161 agttcttttt tgcgttcggc tccggatatg ccgtgggaaa cgatttggta cttcaaaagc
     5221 atagttgaaa acccgtagtc atcctgtgct ttcacctcga gccccacctg catttcttcg
     5281 gataagtcca gatcggagcc ggggcggacg atttcgactg agggcggcgc atcggggagg
     5341 gcggtcagag tgtactcaat cgggtcgcgg ttggcgttcc ccacgtgatc gaccacctga
     5401 atatggtagg tggtgctcct ggtaagcggg aacgggacgg cggcggtttt cccgcgcacg
     5461 ttcatctgtt tgctcgttcc atcgccgaat acgagggtcg ctttatcaac cggtttgttc
     5521 gtttcgaccg aaagcgtgac ccgcgtgccg gtcagggcct ggaggttgcc gtcgttgtcc
     5581 gttttggtca ccgctccgag gcggctgtag gccgggtagg tgagggtggc gcggatgtcg
     5641 gtgacgcggg ggatatcgac aaccgtaatc gcgtacgctt cgctctggaa gttttttccg
     5701 gagaaaaagt aggtgaagtt cttcttcgct tcccggatgg cataggtgaa aacagccgta
     5761 tcgattccgt tcgcggttgg tgacagcgcc tcccggctga gcagttcctt ttgccactgg
     5821 ccgccttcgt tctgccagtt cacgaacagc cggcggggga gattttgccc aatagcagtt
     5881 gccttaagcg ttaccggcgt gaacttcacc acctccaccg agccggggga aaccaaaact
     5941 tggaacggcg gtgattgttt cagttcctcg gtcggatgca gaaaggcgcg gaatgaagcg
     6001 cggaagttgg ccggtgaaac gagcgacaaa agaaatgtag cagccagagt cagcccggcc
     6061 aggcggagcg cttttttgag cggctggcgg tcgagcaccc gggcgaagtc gagattttcg
     6121 gtcttccggt cggcagcaac aattaccgcc gccatcagtt caggcgagta attgggctgg
     6181 tgttcctgca aaaagcgctg gcaatccaac gcggcgacga ggcggttttc cagatcgggg
     6241 aagtgtgctt caaccagccg cgctaaccgg tagaggttcg cccggcgcaa aaacatcggc
     6301 agccagcgga ggccggtgag atagatgaaa gttccgccga ggatgagcca gaccgcaatg
     6361 cggatagagg cgggcagcgt gaacagaaaa gaaaaaacca gaatagtcaa gaaagcgccc
     6421 accaatagcg taatgatgct cgccagcacc cattgagtgg tcaaaagttt ccgggcgcgg
     6481 tcgagcccga ccaaacgttc gactattttc tgaaaactct cattggccat aacgtctcac
     6541 ttcgacgcac taaccttgga cggttctttg ccgcccaaaa gggcccacac ggcgagattg
     6601 gtgcccattt tcagcgcctg ctcccgcttt tcgggcgggt ctttgtgcac ctgcgggtct
     6661 tcccagccgt cgccgatgtc cgactcgtag gcgtagaaga ccaccaacct tccctggtgg
     6721 aggatgccga acccctgggc cggtttgccg tcgtgcttgt gtattttcgg caaaccgttg
     6781 ggaaaatcaa aatacgagtg gtagaggccg tagtcaaagg ggagttccac cagatcggcc
     6841 tccgggaaga ttttttttat ttcccggcgg aaggccttat ccagcccgta ggaatcgttg
     6901 gcgaagagga acccgccgga agtcaggtag gtccgcagtc gattcacttc ggcctcggtg
     6961 aagaaaatgt tcccgtggcc ggtcatatat aagaaggggt agttgaacag gtcgtcatcg
     7021 gccagcgaga cgcggacttc attctcgttc accggaattg aggtgttctc tttaatatag
     7081 cgcaagagat tggggagggc tgatggtccc cagtaccagt ctccccctcc gccatattta
     7141 agccgggcga cggtaaaggt ctgcggatcg gtgagggaag tgactctaac gggctgtgaa
     7201 tgaac
//
LOCUS       DBNGZA010000370         4713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1662637, whole genome shotgun sequence.
ACCESSION   DBNGZA010000370 DBNGZA010000000
VERSION     DBNGZA010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1662637"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="AC16FU_08115"
     CDS             complement(<1..328)
                     /locus_tag="AC16FU_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR2994370.1"
                     /translation="MRPFSTPRWRIFSHLVLPGGLFWLVGLFPGTTARAATTVATLAN
                     GMQVVMKENHVNPVIGLTVYVKAGSKYEGETTNGFTHLLEHLLFDGSRKYSREEINEG
                     MKAHCGY"
     gene            475..1608
                     /locus_tag="AC16FU_08120"
     CDS             475..1608
                     /locus_tag="AC16FU_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR2994371.1"
                     /translation="MSKTHMTFATVCAAIFFASPLFATQFRGGESVVVAKSDSVTDDL
                     FLSCGNAKISGSVTGDLMGFGGSLVFEGPVKGSVMAAGRSVELSGPITGSVRVAGQNL
                     VVNTHVVRNLMAAGQTIHLGSETKIAGDAHLAGEEILIDGQVLGNLYASGARVVILGH
                     IGKNVSLKAEKITLMPTAVIEGNLKYRSRKEAKVEKGALVKGEIKWLPLKRKAPKPFL
                     TAGKVFGKLFFYVAMLIVGSLIIALFRKQTYAVKELALTSFLKSFGIGLLGLVAALVG
                     GLLCLVTLIGAPLALITFTLTFLTVYTTKIFISIAVGDWVLKLFQKTGSPSPYWALVV
                     GLFLVTILVSLPYVGWLFYFVVLFTGFGAILLSLKPLIPQKMG"
     gene            complement(1678..4467)
                     /locus_tag="AC16FU_08125"
     CDS             complement(1678..4467)
                     /locus_tag="AC16FU_08125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2994372.1"
                     /translation="MKRSMVGWFILWVLLFTAAQAQPNFGVTKQVLDNGLVVLVKEKH
                     DIPVVSCMVWYKVGGRNEEIGKTGVSHFLEHMLFKGTKKLKKGDIDLITLKNGGSNNA
                     FTSNDYTAYYFNFASDRWEEALEIEADRMVNNQFLPEEFNPEKQVVIEELKRGNDGPW
                     DQLFEDFQAAAFKAHPYQYPVIGWITDLDKLSIADMEAYYRTFYQPANAILVIVGDVN
                     TEKTLEKVRQLFGTLERRGAIPDMRAVEPEQLGERRIIVHKESNLPRLLVGWHTPKVG
                     EKDSYPLTVLATILSGGKSSRLYQELVEKEKAVTFVNAFYDDARDPSLFAVQAELQPG
                     HELADVEKSIYTQIDRLAAGGISDSELVKAKNQTEAGFVFDQETASGQANALGRAAAI
                     FNYAYAFSYVDSIRKVTREEVVRVARKYLVPDNRTVGWLLPKGGSPESTEQGAKGSTD
                     QFFGAVERDAAAVPAGQVLLQRRKAYKSSDRAYPLQTPPAQTTPPAHSIVAPAKLNVS
                     QKTLGNGLTVLLLERRGNPTVAINAFVKAGSRFDPEEKAGLANLTARMLDEGTTARTG
                     LEIAQAIDFVGGSLSTSGGYAGSTVSAQVLSKDLDLGLNLVADVVRRPVFDSAKVEDE
                     KQKIAAEIKSSLENPRTVGSLQLNELVYGKHPLHRPSVGYEETVAAITRQDVVNFHKF
                     FYLPNDALLAVVGDFKAADVIKKIEKYFGTWEKRSLALPVVPEIARQSDERHRYRYMD
                     KEQINIYLGHLGIKRDNPDYYALEVFDTMLGGGPGFTSRIPKNLRDNLGLAYTTFSNI
                     TGTAGIDPGRFVAYIGTSIPNKDKALEGLKNEIKQFLGGPLGDDELEFAKAYLTGSFV
                     FDFETNSQIAGFLINAVLYNLGFDYLAKYPKLIEAVTKADVARVAKKYIDLDHMSLVI
                     VGKVNERGEVVETKP"
BASE COUNT          923 a         1357 c         1333 g         1100 t
ORIGIN      
        1 tgtaaccgca gtgtgctttc atcccctcgt taatctcttc ccggctgtat ttgcggctgc
       61 cgtcgaagag gaggtgctcc agaagatggg tgaagccgtt ggtcgtttcc ccctcatact
      121 tcgaaccggc cttgacgtac acggttaggc cgatgaccgg attgacatga ttctctttca
      181 tcactacctg catgccgttg gccagagtcg cgaccgtcgt cgcggcccgg gcagttgtgc
      241 ccgggaagag cccgaccagc cagaacagcc ctcccggtag gacgagatgg gagaaaatac
      301 gccaccgcgg agtagaaaaa ggtcgcatgc ccgcaaaaat atgcccgccg cgaagaaaaa
      361 caaaactaaa gcagctggaa accgtaagag aacttgccag cttgcgcacc ctcgtggtat
      421 atatgacctg cgactgagga cgaattatct ccggagcgtt ggaggaggat gactatgagc
      481 aagacccata tgacgttcgc gacggtttgc gcggccatct tttttgcttc tccccttttc
      541 gccacgcagt ttcgcggcgg ggaaagcgtg gtcgtggcca aaagcgactc ggtcaccgat
      601 gaccttttct taagctgcgg caacgcgaaa atttcgggca gcgtgacagg cgaccttatg
      661 gggtttggcg gctcgctcgt cttcgaaggc cccgtaaaag ggagcgtgat ggccgccggc
      721 cgctcggtgg aactctccgg cccgattacc ggttctgtcc gcgtggccgg tcagaacctg
      781 gtggtcaaca cccacgtcgt tcgcaacctc atggccgcgg gccagaccat ccatctgggt
      841 tcggagacca aaatcgcggg cgacgcccat ctggccgggg aagagatttt gattgacggt
      901 caggtgctgg gaaacctgta cgcctccggc gcccgggtgg tcattctcgg ccacattggg
      961 aaaaacgtct cgctgaaagc tgaaaaaatt acactgatgc cgaccgccgt catcgaaggc
     1021 aacctgaagt accggagtag aaaagaagcc aaagttgaga aaggcgcact ggtcaaaggg
     1081 gaaataaagt ggctgccgct gaagaggaag gcgcccaaac cctttctcac cgccgggaaa
     1141 gtcttcggga aattattctt ctacgtcgcc atgctgatcg tcggaagctt gatcattgcg
     1201 cttttccgca agcagaccta tgccgtaaaa gaactggcgt tgacctcgtt cctgaaaagt
     1261 tttggcatcg gtctactcgg gctggttgcc gcgctggtgg gcggtctcct ctgtctggtg
     1321 accctcatcg gcgcgcctct ggcgcttatc accttcacgc tgacgttctt gaccgtctac
     1381 accacgaaaa tttttatcag cattgccgtc ggtgactggg tcctgaaact cttccagaag
     1441 accggttccc cttcccccta ttgggctttg gtcgtgggct tgttcctggt gacgatcctg
     1501 gtgagtcttc cctacgtggg ctggctcttt tactttgtcg tcctcttcac cggtttcggg
     1561 gcgatcctcc tgagtcttaa gcccttgatc ccgcagaaaa tggggtaacc ccgcaagttc
     1621 gggacagaga aaagcgccgg cgggcgcttt tcttttttgc gctgcttgat ggtaaaacta
     1681 cggcttcgtt tcgaccacct ctccccgctc gttcactttg cccacgatga ccagagacat
     1741 gtggtctaaa tcgatgtact ttttggccac gcgcgcgaca tctgccttgg tcaccgcttc
     1801 gatcagtttc ggatatttcg ccagatagtc gaaaccgagg ttgtagagca ccgcgttgat
     1861 caaaaaaccg gctatttggg aattggtttc gaaatcgaac acgaaactgc cggtgaggta
     1921 ggccttggca aactctaatt catcgtcgcc cagaggcccg cccagaaatt gcttgatctc
     1981 gtttttcagc ccttcgaggg ctttgtcctt gttcggaatc gacgtgccga tgtaggccac
     2041 aaagcgtccc gggtcgatgc cggcggtacc ggtgatgttg gagaaggtgg tgtaggccaa
     2101 ccccaaattg tcgcgcaggt ttttggggat gcgcgaggtg aaccccggcc cgccccccag
     2161 catcgtgtcg aacacttcca gagcgtagta gtccgggttg tcgcgcttta tgcccaggtg
     2221 tcccaggtag atgttgattt gctctttgtc catgtagcgg taccggtgcc gctcgtcgct
     2281 ctgccgggca atctccggca ccaccggcag ggccagcgac cgcttctccc acgtaccgaa
     2341 gtatttctct attttcttta tgacgtcagc ggctttgaaa tcgccgacga cggccaaaag
     2401 tgcgtcattc ggcaggtaga agaatttatg gaaattcacc acgtcttgcc gcgtgatggc
     2461 ggccaccgtc tcttcgtacc ccaccgacgg ccggtggagg gggtgcttgc cgtaaacgag
     2521 ttcgttcagt tgcagtgatc cgaccgtgcg cggattctcc agagaacttt tgatctccgc
     2581 agcaatcttc tgtttctcat cctcgacttt ggccgaatcg aacaccgggc gtcggacgac
     2641 gtcggccact aagttcaagc ccagatcgag gtctttgctc aacacctggg ccgagacggt
     2701 ggaaccggcg tagcccccgg aggtggaaag cgacccgcct acgaagtcaa tcgcctgagc
     2761 gatctcgaga ccggtgcggg cggtggtgcc ttcgtctaac atccgcgccg tcaggttggc
     2821 taaacctgct ttttcctcgg ggtcaaagcg tgacccggct ttgacgaagg cgttgatcgc
     2881 cacggtcgga ttgccccggc gctccaggag caaaacggtc aggccgttgc ccaaggtctt
     2941 ctggctgacg tttagtttcg ccggagcaac tatggagtga gccggaggtg tggtttgggc
     3001 tggcggagtt tgaagggggt aagcgcgatc cgaggactta taggccttgc gccgttgcaa
     3061 caaaacttga cccgctggaa ccgccgcggc atcccgctcc actgctccga aaaattgatc
     3121 agttgatccc ttggcccctt gctccgttga ctctggagaa ccacctttgg gcaggagcca
     3181 ccctaccgtc cggttgtccg gaaccagata tttccgggcg acgcgaacga cttcttctcg
     3241 ggtgactttg cggatgctgt cgacgtagct gaaggcgtag gcgtaattga agatcgccgc
     3301 ggcccggccc agggcgttcg cttggccgga ggcggtttcc tggtcgaaga caaagcccgc
     3361 ttcggtctga tttttggctt tgaccaattc gctgtccgaa attccacccg cggcgagccg
     3421 gtcaatttgg gtgtaaatcg atttctccac gtccgccagt tcgtgccccg gctgcaattc
     3481 ggcctgaacc gcgaagagac tggggtcgcg ggcgtcgtcg taaaacgcat tgacgaacgt
     3541 gaccgctttc tctttttcta ctaactcttg atagaggcgc gaactcttcc ccccggagag
     3601 gatggtggct aaaacggtca gggggtagct gtccttctcc cccactttcg gtgtatgcca
     3661 cccgaccaga agccgcggga gattcgactc cttgtggaca ataatccgtc gctcgcccaa
     3721 ttgctccggc tccaccgccc gcatgtcggg gattgctccc cgccgctcga gggtcccgaa
     3781 caattgtcgg actttttcca gggtcttctc cgtattgacg tcgcccacga taactaagat
     3841 ggcgttggct ggctggtaga aggtgcggta gtaggcctcc atatcagcga ttgagagctt
     3901 gtctaaatcc gtaatccagc cgatcaccgg gtactggtag gggtgggctt tgaacgcggc
     3961 cgcctggaag tcctcgaaga gctgatccca cggcccgtcg ttgccccgct tgagttcttc
     4021 gattaccacc tgtttttccg gattgaattc ttcgggaaga aattgattat tcaccatccg
     4081 gtcggcttca atttccaagg cctcttccca ccggtcggag gcgaagttga agtagtaggc
     4141 ggtgtagtcg ttggaggtga aggcgttgtt cgagccgccg ttcttgagcg tgatgaggtc
     4201 gatgtcgccc ttcttcaact tcttggttcc cttgaagagc atgtgctcca gaaagtgcga
     4261 gacgccggtt ttgccgattt cttcgttgcg cccgcctacc ttgtaccaga ccatgcagga
     4321 gaccaccggg atgtcgtgct tctccttcac gagcaccacg aggccgttgt ccaaaacttg
     4381 cttggtgacc ccgaagttcg gctgcgcctg agccgccgtg aaaagcaaaa cccagaggat
     4441 gaaccacccg accatactgc gcttcatcaa ctacctccct tttcctgcgt cccgagtgcc
     4501 ggaaacgttc cttccagttg gtttcagtat ggcgtcgcct gctccggttg tcaaatttcc
     4561 ggccctactg aacgcctaag tccgacagca ggtgctcccg taccagaccg gctgagtcaa
     4621 acattcagcc cacagtcgtg aaaaaaaacc gccgtcagct ctgcaaccga cggcggtttc
     4681 tcgtaacgac ttgaattatt tgatctgccc gat
//
LOCUS       DBNGZA010000371         2247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2722477, whole genome shotgun sequence.
ACCESSION   DBNGZA010000371 DBNGZA010000000
VERSION     DBNGZA010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2722477"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC16FU_08130"
     CDS             complement(<1..868)
                     /locus_tag="AC16FU_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994373.1"
                     /translation="MPLDFAIETSDLSKSFDSFTAVDRVTLSVRPGEIFGLVGPDGAG
                     KTTTIRLLVGVLSITSGRARVAGFDLTQEPDEVKKRIGYMSQRFALYGELTVDENIRF
                     FAEIYRVPKAEREALTKRLLDFSKLAPFRTRQARHLSGGMKQKLGLCCALVHTPEVLF
                     LDEPTTGVDPVSRRELWDILYDLLEQKVTIFVSTPYMDEAERCSRVAFLSGGRVLTCD
                     TPSALKKNFPEKVWEVTCSDSQRASELLDGVDFVSGVTVFGERVHVVTPTEENVVQRI
                     SALLAAQGITVQN"
     gene            complement(1244..>2247)
                     /locus_tag="AC16FU_08135"
     CDS             complement(1244..>2247)
                     /locus_tag="AC16FU_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994374.1"
                     /translation="DGDGKADFIIGAPNAEPGGFSSSGSAYVYSGATGGLLYQFNGIA
                     DSMFFGYPAVGVGDVDADGRPDFIVGAIGASPSGLTDAGSVYVYSGVNGSLIYQKDGS
                     APGDQFGYSVDGVGDLDGDSKSDFMVGAPFADPNEVNLAGSVFVYSGATGGLLFQKNG
                     AAVDDHLGWRVAGSGDIEGDGKPDFMIGAPLAHSGGFRWAGSAFIYSGATGDLLFRKV
                     GSAGDYMGNSVAGTGDVNGDGRSDFIIGAPFADPGGLTNSGSAFVMTCAGLCGDVDND
                     SSTTIADIIALINNVVFETGVANPTAGDTNCDEVRNIEDIVLLIQYVVFGTPTPCCL"
BASE COUNT          575 a          692 c          550 g          430 t
ORIGIN      
        1 tattctggac ggtaatcccc tgagccgcta aaagcgcgga aatcctctgc accacgttct
       61 cctctgtggg cgtgacgacg tgcacccgct cgccgaaaac cgtcaccccg ctgacgaaat
      121 cgacgccatc aagcagttcg gaggcgcgtt gagagtcgga gcaggtcacc tcccacacct
      181 tttccggaaa attctttttc agagctgacg gcgtgtcgca ggtcaaaact ctgccgccgg
      241 agagaaaggc cacccggctg caccgctccg cctcatccat ataaggcgtg gagacgaaga
      301 tcgtcacctt ctgctctaag aggtcgtaca ggatgtccca cagttcccgg cgggagaccg
      361 gatcgacgcc ggtagtcggc tcgtccaaaa agagcacctc cggcgtgtgg acgagggcgc
      421 agcagagccc caacttctgc ttcatcccgc ccgataagtg ccgcgcctgc cttgtgcgga
      481 agggcgctaa cttggaaaag tctaacagcc gtttggtcag cgcttcacgt tcggctttcg
      541 ggacgcgata gatttctgca aagaaacgga tattctcatc cactgtcaac tcgccgtaca
      601 gcgcgaaccg ctgggacata tacccgatcc gctttttgac ttcatccggc tcctgcgtta
      661 aatcaaatcc cgccacccgc gccctgccgg aagtgatcga gagcaccccg accaaaagcc
      721 ggatcgtcgt cgtcttcccc gccccatccg gccccaccag gccaaagatt tctccgggac
      781 ggacagaaag cgtcacccga tcaacagcgg tgaaagaatc aaaagatttg ctgaggtcgg
      841 aggtttctat ggcaaaatca agaggcatgg tttggagaga atttccctcc ccttgagggg
      901 agggctgagg gaggggttat accataaacc ggcacattgc tgaaattatg ttagttccct
      961 tgagctatca aactgatttt ctctgacctc tcccgtccct ggcgggacac cctctcctca
     1021 aaggagaggg agagatctca acccaacctt cccctgcaag aatgaggtga cctcacccca
     1081 accctctcct acaggagagg gagacaagaa aattccttcc tcttcgaagg gagagaggcc
     1141 gaagacccgc ctcaggcggg gaggggtcaa caataattac aaacaacagg gagtggggtt
     1201 atggttcgat ccgcctcagg cggactcacc acacacccaa cctttacaaa caacagggtg
     1261 tgggcgtacc aaacaccaca tactgaatga gcagcacaat gtcctcgata ttccgcactt
     1321 catcgcaatt ggtgtcgcct gctgtggggt tagccactcc ggtctcaaac accacattat
     1381 tgatgagagc aatgatgtcc gctatggtag ttgaactgtc attgtccaca tccccgcaca
     1441 ggccagcgca ggtcatgaca aaggccgaac cggagttagt tagtccaccc gggtctgcga
     1501 agggagcacc aataatgaaa tctgatcgtc catccccatt cacgtctccg gtccccgcca
     1561 ctgaattacc catatagtcg ccagcagacc cgacttttcg aaagagaagg tcgccagtcg
     1621 cccccgagta gataaaagca gaaccagccc agcgaaaacc accagagtgg gctaagggcg
     1681 ctccgatcat aaagtcgggc ttgccgtcgc cttctatgtc accggatccc gccactctcc
     1741 accccaaatg atcatcgacc gcagcaccat ttttttggaa aagcaaacca ccagtggctc
     1801 cagaatagac aaataccgat ccagccaaat tcacttcgtt cggatcggca aaaggagcgc
     1861 caaccataaa atcgcttttg ctatcgccat ccaaatctcc aacaccatcc acagaatagc
     1921 caaactggtc cccgggggca ctaccatctt tctggtaaat cagggaaccg ttcaccccgg
     1981 aatacacgta aaccgaaccg gcatcagtta gaccacttgg gctggcgcca atagcgccga
     2041 cgatgaaatc aggtcgtcca tcggcatcta cgtcaccaac cccgaccgca ggataaccga
     2101 aaaacatact gtcagcgatc ccgttaaatt ggtaaagcag ccctccagta gcaccggagt
     2161 agacatatgc cgaaccagac gaggaaaacc cgcccggctc agcattggga gcgccgatga
     2221 taaaatcggc cttgccgtcg ccatcca
//
LOCUS       DBNGZA010000372         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2829904, whole genome shotgun sequence.
ACCESSION   DBNGZA010000372 DBNGZA010000000
VERSION     DBNGZA010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2829904"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16FU_08140"
     CDS             <1..423
                     /locus_tag="AC16FU_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HIR2994375.1"
                     /translation="VAINVAISPWLVVCAILLALFLGFSKRRHELLLLEGSAEEHRKI
                     LREYSPEFLDQLISVVTASTVVAYAFYTLSPEVRTKLHTPYLPLTIPFVLYGIFRYLY
                     LTHQKIEGGDPTRTLLSDRPLVLNTFLWVAAVLVILYF"
     gene            complement(454..1572)
                     /locus_tag="AC16FU_08145"
     CDS             complement(454..1572)
                     /locus_tag="AC16FU_08145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2994376.1"
                     /translation="MRIEEVATQSQMRAFIKFPWRIYRGNPHWVPPLLVERKEFFNPR
                     KNPFFQHAQVKYWLAYRDREVVGRIAALVNQAHNDYHQEKCGFFGFFEAVNDPDVARA
                     LYGTAENWLKAQGMALMRGPANFSTNDEVAFLLEGFDHPPTLMMPYTPPYYNELAEGF
                     GLRKAKDLYGYLIRRENLPTERILRVAERIKKKEGIVVRPLRMKEFDREVARVKEIYN
                     AAWSKNWGFVPMTDAEFNYIARQFKQIVDPDLTLIAEVGGKPAGFCLALPDINQALIR
                     LNGRLFPWGLVKLLWLTKVKKIITGLRLVTMGVVPEFQKRGIDTIFFIETFRTGVAKG
                     YRWAELSWILEDNTLMNRAAEAFGAELYKKYRIYEKPL"
     gene            complement(1661..>2446)
                     /locus_tag="AC16FU_08150"
     CDS             complement(1661..>2446)
                     /locus_tag="AC16FU_08150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2994377.1"
                     /translation="RLAFGKTIKYRHNDMDDLKRVLASLGKGTGKLIVVDGVFSMEGD
                     IAKLPEICDLAHRYNARTLVDDAHSIGVLGPGGRGTAEHFGLEDEVDIIMGTFSKSFA
                     SLGGFIVAKEEVIHYIKHFSRELIFSASMPPAAVAACLAALDIIETEPQRRARLWEIT
                     RRVHRELRGLGFDIGVTETPIVPIYIGADMRTFQFWKALTDAGVFSNPIVSPAVPKGR
                     ALIRTSYTATHTDEQIDKVLGLLAEVGQRFGILKPQPVPVGDA"
BASE COUNT          478 a          716 c          704 g          548 t
ORIGIN      
        1 gtggccatca atgtcgccat atccccctgg ctcgtggtct gcgccattct cctggcactt
       61 tttttgggtt tcagcaaacg ccggcacgaa ttgcttcttt tggaagggag cgcagaggag
      121 caccggaaaa tcctgcggga atactccccc gaattcctcg accagctgat cagcgtggtg
      181 accgcttcaa cggtagtggc gtatgctttc tacacccttt cgccggaagt acgcaccaaa
      241 ctccacaccc cctatctgcc cctgaccatc ccctttgtcc tctacgggat tttccgctac
      301 ctctacctca cccaccagaa aatcgaaggg ggggatccga cgcggaccct tctcagcgac
      361 cggccgctgg tgctcaatac gtttttgtgg gtggccgcgg tgctggtgat cctctacttc
      421 taaaaaaact gcggacttag acggtcaaat cactcatagc ggtttttcgt aaatgcggta
      481 cttcttatat aactcagccc cgaaggcttc cgccgcgcgg ttcatcaatg tattatcttc
      541 cagaatccag ctcagttcgg cccaccggta ccccttggcc acgccggtgc ggaaggtttc
      601 gataaagaag attgtgtcta tgccgcgttt ctgaaattcc ggtaccacgc ccatggtgac
      661 caggcgcagg ccggtgatga tttttttgac tttggtcagc cagaggagtt tgaccaatcc
      721 ccaggggaaa agccgtccgt tcaaccggat cagcgcctgg tttatgtcgg gcagggccag
      781 gcagaagccg gcgggcttcc cccccacttc ggcaatgagc gtcaaatccg gatcgacgat
      841 ctgcttgaac tgccgggcga tgtaattgaa ctcggcgtcg gtcatcggca caaagcccca
      901 gttcttgctc caggcggcgt tgtagatttc cttcacccgg gccacttcac ggtcaaattc
      961 tttcatccgc aacgggcgaa cgacaatgcc ttctttcttt tttatgcgct cggccacccg
     1021 gagtatccgc tcggtgggca gattctcccg ccgaatcaga tacccgtaga ggtctttggc
     1081 tttccggaga ccaaagcctt cggctaattc gttgtagtag ggcggggtgt agggcatcat
     1141 aagcgtcggc gggtggtcga agccctccag gagaaaagcg acttcatcgt tggtcgaaaa
     1201 gttcgccggg ccgcgcatga gagccatccc ctgcgccttg agccagtttt cggcggttcc
     1261 atagagagcc cgggcaacgt cgggatcgtt gaccgcttca aagaagccga agaagccgca
     1321 cttttcctgg tggtagtcgt tgtgcgcctg gttgacgagg gcggcgattc gtccgaccac
     1381 ctcccgatct cggtaggcca accagtattt gacttgagcg tgctggaaga aaggattttt
     1441 ccgggggtta aaaaattcct tccgctccac taaaagcggc ggcacccagt gcggattgcc
     1501 ccggtaaatg cgccagggga atttaatgaa agcccgcatc tggctttggg tggcgacttc
     1561 ttcaatccgc atcacgggag gtgattctct cggtagagca gttctgtttt aatggaggaa
     1621 gtgggccggg agttgtccgg cggtcgcggc ggctggagaa ttacgcatct ccaacgggta
     1681 ccggttgcgg cttgaggatg ccgaagcgct ggcccacttc cgccaggagg cccagcactt
     1741 tgtcaatctg ctcgtccgta tgggtggcgg tgtaactggt acggatcaag gcccggcctt
     1801 tgggcacggc gggggaaacg atcggattgc tgaatacgcc cgcatcggtt aacgccttcc
     1861 agaattggaa cgtgcgcata tccgcaccga tgtaaatcgg cacaatgggg gtctcggtca
     1921 ccccgatgtc aaaccccagc ccccgcagtt cgcggtgaac ccggcgggtg atttcccaca
     1981 gccgggcgcg cctctggggc tcggtctcga taatgtctaa cgccgccagg caggccgcca
     2041 ccgcggccgg cggcatgctg gcagagaaga tcaattcccg tgagaagtgc ttgatgtagt
     2101 ggatgacttc ttctttagcc acgataaagc cgcctaggga ggcgaacgat ttgctgaaag
     2161 ttcccatgat gatgtcgacc tcatcttcca ggccgaagtg ttcggcggtg ccccggccgc
     2221 cggggcccaa aacgccaatc gagtgagcgt cgtccaccag ggtacgggcg ttgtaccggt
     2281 gcgccaggtc gcagatttcc ggcagtttgg cgatgtcgcc ctccatactg aagacgccat
     2341 cgaccacgat caatttcccg gtgcccttgc ccaaagaggc cagcacccgc ttgaggtcgt
     2401 ccatgtcgtt gtgccggtac ttgatcgtct tgccgaaggc cagccg
//
LOCUS       DBNGZA010000373         4588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2723021, whole genome shotgun sequence.
ACCESSION   DBNGZA010000373 DBNGZA010000000
VERSION     DBNGZA010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2723021"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..626)
                     /locus_tag="AC16FU_08155"
     CDS             complement(69..626)
                     /locus_tag="AC16FU_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2994378.1"
                     /translation="MTEFFHNLDTQLFIFLNATLANPVFDRVMPVITNSFYWRLPLAA
                     ILLYLLIWGKKKGRITVLLFVLTAVISDQISSHLIKSFIHRLRPCHVVPQVHLLVNCV
                     HSYSFPSSHAVNSFAFAIMFWRRYPQSKYFFFPIVAIIAYSRVAVGVHYPFDILGGAL
                     IGIGVGLGLFWLYRKASRRWRWEGV"
     gene            complement(722..1108)
                     /locus_tag="AC16FU_08160"
     CDS             complement(722..1108)
                     /locus_tag="AC16FU_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR2994379.1"
                     /translation="MKRQNVSSGSPFESIIGFSRAVRVGHHVLVSGTAPIGADGKTVG
                     IGDAYVQTKCCLEIIAQALEKAGARLEHVVRTRTFLVNIDDWEKVARAHGESFATTRP
                     TSTFVQVSRFINPEWLVEIEADALIE"
     gene            complement(1185..1829)
                     /locus_tag="AC16FU_08165"
     CDS             complement(1185..1829)
                     /locus_tag="AC16FU_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HIR2994380.1"
                     /translation="MTATKDTYQTIGAPCSARLKIQGSIFFARAVPIASVTEVTPFLT
                     SLKKELHDATHHCWAYKLDDGVSARVKWSDAGEPKDTAGRPILQAIESRDLTNLLVVV
                     SRWFGGTKLGKGNLSRAYRQAAEQALANAPIVTGCWRTRLRLSVSVEQVSAVYAVAGR
                     FAGRVQVEASDNGGRFTVAVPRSETEKFLELLMERTAGKIQFEYTHAGKAKKRT"
     gene            complement(1834..2727)
                     /locus_tag="AC16FU_08170"
     CDS             complement(1834..2727)
                     /locus_tag="AC16FU_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR2994381.1"
                     /translation="MTYSEQTYVKALRERGKVGPKTFQMLLYHFGSPEAILNTPWEEI
                     AALPRLNEKRAKEILGARDWLEDAALELNALAGDQIRLMTFLEDGYPERLKAISDPPP
                     LLYLKGEFPVPSERFVAVVGTHEATAEGIAAAVQFGKVLAGAGAVVVSGLARGIDAAG
                     HVGALKAGGKCYGVLGSGFGQIYPKEHDSLASEVAKNGALISEYAPGVPVNVGQLMAR
                     NRIVVGLASAVIIVELPGPDGAEQNNSGTLNAMERGREQGKPVYLYDPQKHLSAEQLH
                     QWEAQSLIGPEQLSELLPYLI"
     gene            2872..3786
                     /locus_tag="AC16FU_08175"
     CDS             2872..3786
                     /locus_tag="AC16FU_08175"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HIR2994382.1"
                     /translation="MAQRKRTPAIIVHGGAWDIPDDLVKPHLDGCRRAVEAGYSLLRE
                     GASALDAVVAAVKVMEDDPAFDAGVGAFLNAEGEIELDASLMDGRTLGCGAVAGVKTV
                     KNPIELARLVMERTEYVMLIGSGAEAFARQQGIPACRFEDLLVPRERERWQEFKRRKN
                     FSARGVFATKPHGTVGAVAVDSEGNLAAANSTGGTPNKLPGRVGDTGQIGSGTYADNE
                     KGAACSTGWGEPIIKIVMAKAICDRMAKKTAPKAAINQALNELRKRVGGFGGAIAIDR
                     TGRVGLAFNTPRMAFAYSNVQGDIVTGI"
     gene            complement(3788..>4588)
                     /locus_tag="AC16FU_08180"
     CDS             complement(3788..>4588)
                     /locus_tag="AC16FU_08180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR2994383.1"
                     /translation="RKSSLTLRVNTFRTSVEKIIAQLEQVGVRVMPGKFFPQFLTPKS
                     EYRFFDSPWFKNGFFYVQDESAAAAVHLLDPKPGETIWDACAAPGGKATHVAELTGDT
                     GTVLASDLSLRRTKLIKENSTRLRLNSVIAVVADGLSYHPKGMRKILLDVPCTGLGTV
                     ARNPDLRWHKKPEDIPRLAQLQWELLTNAVMSLPVGGVLVYSSCTLTREENQELMEKF
                     IHTHPRYRLEPAGEFVPPELVDGFGYVAILPQKHGIDGAFAARFRRIW"
BASE COUNT          994 a         1298 c         1321 g          975 t
ORIGIN      
        1 cgcgttgacc agggcgtcgg cctccacgtc ggcgatgttc ccctgggtca gttcgatagc
       61 gattttgttc atactccttc ccatcgccaa cgacgactcg ctttacgata aagccagaag
      121 agtcccaggc caactccaat gccaataaga gcaccgccca ggatgtcgaa cggatagtga
      181 acccccaccg ccacgcgcga gtaggcgatg atcgcgacta tcgggaaaaa gaaatacttc
      241 gattgcgggt agcgccgcca gaacattatg gcgaacgcaa acgaattgac cgcatgggat
      301 gacgggaacg aatacgaatg gacgcagttg accaaaagat gcacctgcgg caccacgtgg
      361 caggggcgga gccggtggat gaacgacttg atcaggtggc tggaaatctg gtcactaatt
      421 actgccgtga gaacgaaaag aaggacggtg atccgtcctt tcttcttccc ccatatcaag
      481 agataaagca ggatggccgc cagaggcagc cgccagtaga atgagttggt gatcaccggc
      541 atcacccggt cgaaaaccgg attggcgagg gtagcgttca aaaagatgaa aagttgcgtg
      601 tcgagattgt ggaaaaattc agtcaatgca ctaccctgat catttccgct tgaagcaatt
      661 attcagattt cccattccgc gactctcgca ccggggtctg atgaaagcgg gtacaaattt
      721 tttactcaat tagtgcatcc gcttcgattt cgaccaacca ttcaggattg atgaagcgac
      781 ttacttgaac aaatgtggaa gtcggtcgcg tcgtcgcgaa gctctctcca tgggcacgcg
      841 caaccttttc ccagtcgtca atgttcacta agaaggtgcg ggtgcggacc acatgttcaa
      901 gccgggcacc ggctttttcc aatgcctgtg cgataatttc caggcaacat ttcgtctgaa
      961 cgtaggcatc tccaattccc accgtctttc catcggcacc aatgggggct gtgcctgaaa
     1021 caagaacgtg gtgcccaaca cgcacggcgc gggaaaaacc gatgatgctc tcaaaaggag
     1081 agccggaaga cacattctgc cgtttcattc tggttgctcc tcctctccat aagcgagttg
     1141 acttgacagc atagcaaaat tcatccggta gggcgctccg tttcctatgt tcgttttttc
     1201 gcctttcccg cgtgggtgta ctcgaattga attttaccag cggtgcgttc catcaaaagt
     1261 tccagaaatt tttcagtttc actccgggga acggcgacgg tgaaacgtcc accgttgtcc
     1321 gatgcttcga cctgcacccg gccggcaaag cggcctgcca cagcgtaaac ggcactcacc
     1381 tgttccaccg aaaccgaaag gcggagccga gttcgccagc agcccgtcac gataggtgcg
     1441 ttggccagcg cctgctccgc cgcctgccga taggcccggc tgaggttgcc cttgcccagt
     1501 tttgtgccgc cgaaccaacg gctgaccacc accagaaggt tggtcagatc ccgtgattcg
     1561 atggcttgca gaataggcct gccggcggtg tctttcggct caccggcatc agaccatttt
     1621 acccgggcag aaacaccgtc gtccagttta taagcccagc aatggtgggt ggcatcgtgg
     1681 agctcttttt tcaaactggt aaggaacggc gtcacctccg taacgcttgc aatcggtacc
     1741 gcccgggcga agaaaatcga gccctgaatt ttcagtctgg cgctgcacgg cgcgccgatt
     1801 gtctggtagg tgtcttttgt ggctgtcaat tgactaaatc aggtaaggga ggagttcgga
     1861 caactgttcg ggaccgatca gcgattgcgc ctcccattga tgcagctgtt cggcggagag
     1921 gtgcttctgc ggatcgtaca ggtagaccgg cttgccctgc tcccgccccc gttccatagc
     1981 gttcagcgtt ccggaattat tttgctccgc gccatccggc ccggggagtt ccacgataat
     2041 cacggcgctg gcgagcccca ccacgatgcg attgcgggcc atcaattgcc cgacgttgac
     2101 cggcacccca ggcgcatact ccgaaatcaa ggcgccgttt ttggcgactt cggaggcaag
     2161 cgaatcatgc tctttcggat agatttgccc aaagccggaa cccaagacgc cgtagcactt
     2221 gccccccgct tttagcgccc ccacgtgtcc ggcagcgtcg atgccgcgcg ccaggccgct
     2281 cacgaccacc gcaccggccc cggccagcac tttgccgaac tgcaccgcgg cggcaatccc
     2341 ttcggcggtc gcctcgtgcg ttcccacgac ggcgacaaag cgctcgctcg gtaccggaaa
     2401 ttcgcctttg aggtagagca aaggcggcgg gtcgctgatg gctttcaacc gttccgggta
     2461 gccgtcttct aagaacgtca tcaatcgaat ttggtctccg gccagggcgt tcagttccag
     2521 cgcggcgtcc tccagccagt cgcgtgcgcc taagatttct ttggcgcgtt tttcgttcaa
     2581 gcgcggcagt gcggcaatct cctcccaggg ggtgttgagg atcgcttccg gcgagccgaa
     2641 gtggtacagg agcatctgaa acgttttggg ccccaccttg ccccgctccc gcagggcttt
     2701 gacgtaggtt tgctcggagt aggtcatcac cggaacccgc cgaagatttg actcaacata
     2761 tgacaaacca taataaagag tcgtttattt acggctgcaa gtcagcaaca gtcgttccac
     2821 cagaaaatat cgcttgactt tcatccgaaa ttctttttcc tgaagcgctt aatggcccag
     2881 cgcaagcgca ctcccgcgat catcgtccac ggcggagcgt gggatattcc ggatgacctg
     2941 gtcaaaccgc atctggacgg ctgccggcgg gcggtggaag ccggttattc cctgcttcgg
     3001 gaaggcgctt cggctctgga tgcggtagtg gcggcggtca aggtgatgga agacgatccc
     3061 gcgttcgacg cgggtgtcgg cgctttcctg aacgcggagg gtgaaatcga actagatgct
     3121 tcgctgatgg atggcaggac gctcggctgc ggggcggtgg cgggcgtcaa aaccgtaaaa
     3181 aatccgattg aactggcccg gctggtgatg gagagaaccg agtacgttat gttaatcggc
     3241 agcggggccg aagcttttgc ccgccagcag ggtattcccg cctgccggtt tgaagatttg
     3301 ctcgtccccc gcgaacggga gcgctggcag gagttcaaac gccggaaaaa cttttcggcc
     3361 cgaggggtct ttgctaccaa accgcacggg acagtggggg cggtagcggt cgatagtgaa
     3421 ggcaatttag ccgccgcaaa ctccaccggc ggaacgccca acaaactgcc gggccgggtg
     3481 ggtgacaccg gccagatcgg ctccggcacc tacgcggaca acgagaaagg cgccgcctgc
     3541 tccaccggct ggggcgagcc gatcattaaa atcgtcatgg cgaaagcaat ttgcgaccgg
     3601 atggccaaga aaaccgctcc gaaggcggcc atcaaccagg ccctgaatga attgcggaaa
     3661 cgggtcggcg gctttggcgg ggccattgcc attgaccgga cgggacgggt gggtttggcc
     3721 ttcaataccc cgcgaatggc gtttgcgtac agtaatgtcc agggagatat cgtaaccgga
     3781 atttgaatta ccagattctc cggaaccgcg cggcaaaggc gccatcaatc ccatgttttt
     3841 gggggagaat cgcaacgtag ccgaagccgt cgaccaattc cggcggcaca aattcacccg
     3901 ccggctccag ccggtagcgg gggtgggtat ggatgaattt ttccatcagt tcttggtttt
     3961 cttcccgggt caaagtacag ctgctgtaga ccagaacacc gcccaccggc aggctcatga
     4021 ccgcgttggt caaaagttcc cattgcagtt gtgccagacg ggggatgtct tccggttttt
     4081 tgtgccagcg taaatctgga ttgcgtgcca ctgtccccag accggtgcag ggaacgtcga
     4141 gcaaaatttt tcgcatccct ttcggatggt aggagagacc gtcggcaacg accgcgataa
     4201 cagaattcaa cctgaggcgg gtgctgttct ctttaatgag tttggtgcgc cgcagactga
     4261 ggtcgctcgc cagaaccgtg cccgtatcac cggttaactc agctacgtga gttgccttgc
     4321 ctcccggcgc ggcacaggca tcccaaatcg tttcccccgg ttttggatca aggaggtgca
     4381 cggcggctgc ggcgctttca tcctgtacgt agaagaagcc gtttttgaac cagggggaat
     4441 cgaaaaaacg gtattcggat ttaggagtta ggaattgggg aaaaaacttt ccgggcataa
     4501 ctctgacgcc gacctgctcg agttgcgcga ttattttctc aacactggta cggaatgtat
     4561 tgacccgcag ggtcaaagag gacttacg
//
LOCUS       DBNGZA010000374         3191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1238307, whole genome shotgun sequence.
ACCESSION   DBNGZA010000374 DBNGZA010000000
VERSION     DBNGZA010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1238307"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="lnt"
                     /locus_tag="AC16FU_08185"
     CDS             <1..1176
                     /gene="lnt"
                     /locus_tag="AC16FU_08185"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR2994384.1"
                     /translation="GSWAIVLFPFFWVAHEYLRSLGELGFPWTNLSYTQTYYPHLIQY
                     ASYTGDLGVSFWIVLVNVAVFGLWRFWTKRRTMLRLAMGLAILFLTPFVYGYLVIPRK
                     TAGSGVPVALLQANIDSNLKWDQAYTASSFAIFDTLAGEATAKGARLLIWPESSAPCY
                     LRAEPVWLLHLQRQAAELKKPMLVGALDYESPAAGKYIFYNAAFQFDSLGNLSPPYRK
                     IHLVPVGERIPFRDEVTVLDKIQLGQADFTSGKEFTTFAAPFGRYGCLICFESVFPDL
                     VRRFVLSGADFLVNITNDAWFGRTSGPYQHAQMVVFRAIENRISIARSANSGVSLVVD
                     RWGRISARSPIFAREVVVAPLDPAHERTFYTAHGNWFAKLASAVSLVFLGLAVFRKK"
     gene            1176..>3191
                     /locus_tag="AC16FU_08190"
     CDS             1176..>3191
                     /locus_tag="AC16FU_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR2994385.1"
                     /translation="MRILPELEKQMLRCMLGAVVILAPFAESFAQTWLNFTNKNSIVD
                     VAAGPGVVWCATTGGALRFDLATDRIDQFTTAEGLGGTSLLSVAVDTSGTVWFGGNNG
                     TLTKYEARTNRWTVFPFIDRDNRKLKLKDIFIDGNKLWMATDVGVILFDAVRNGGEIR
                     ETYRRLGTLQQEAVNAVLVKDGRIWAAAVGGVVWAFTSDPNLLDPARWHTFTKTTGDS
                     LQGDQYQALAAFDSTVFVGSEAGLFRLVEMPADTFWQVLPVASDLIRALHAAGDSLFF
                     SGRFGLGVVTRPGLTVSFLNSDPLGTRIFPGLAMATDGRLFIATQGKGLGIYRNQTYQ
                     RRVIDGPPENFVVDVALDRRGRVWIANWNSGFAMYDGTWHTFDTLPVAPLGWAVTVDL
                     QDNVWCCSWGGGIYEFTSDGRFRAFTSANSPVRGIASTSAYTVSTDAVVDFQGNVWFT
                     NPYAYDSTALLAFRPDSSWEVFYKNARDGLVSNDIVMLYPQDDHIWIGYKSAGTAAGV
                     GDFNFNGTLGNHADDILVFYNQEREKLLSNTVTAIAIDRTGVLWVGTTAGLQRFDPDL
                     RRFLGVQMPSPLGPQVKAITIDARNNKWIGTSTGLGLITPDGQFRPPILSTNSGLVSD
                     AVAEITLDPVTGDLWIAAGSPFSGEEGGLSRFRTGFGGTVSDLKQVEA"
BASE COUNT          573 a          896 c          990 g          732 t
ORIGIN      
        1 ggcagttggg ctattgttct tttccctttc ttctgggtgg cgcacgaata cctgcgctcg
       61 ttgggagagt tggggtttcc ctggacgaac ttgagttata cgcaaacgta ctacccccac
      121 ctaatccagt acgccagtta caccggtgat ctcggagtgt cattctggat tgtgctcgtg
      181 aacgtggcgg tgtttggtct ttggcgtttc tggaccaagc ggcggacgat gctccgtctg
      241 gcaatgggac tggcgattct ttttttgacg cccttcgtgt acggctatct cgtgattccc
      301 cggaagaccg ctgggtccgg cgtaccggtg gcgctcctgc aggcgaacat cgattcgaac
      361 ctcaaatggg atcaggccta caccgcctca agtttcgcca tcttcgacac tctggccggg
      421 gaggcgacag ccaagggggc gcgtttgctc atctggcccg aatcgtcggc gccctgttac
      481 ctgcgggcgg agccggtctg gcttttgcac ctccagcggc aggcggctga actgaaaaag
      541 ccaatgttag tgggcgcgct ggattatgaa agcccggctg cggggaaata catcttttac
      601 aatgccgcct ttcagttcga ttcgctgggg aatctgtcgc cgccctaccg gaagatacat
      661 ttagtgccgg tcggagaacg aattcctttc cgggacgagg tcacggtgtt ggataagatt
      721 cagttgggtc aggccgattt cacgagcggg aaggagttca ccaccttcgc cgcgccattt
      781 ggaagatacg gctgtctcat ctgcttcgag tcggtttttc ccgatttagt tcggaggttc
      841 gttctgtcgg gcgccgattt tttggtgaac attaccaacg acgcctggtt cggccgcacc
      901 tccgggccat accagcatgc ccaaatggtg gtcttccggg cgatagagaa ccggatcagc
      961 attgcccgct cggccaacag cggcgtatca ctggtggtcg accggtgggg acgtatctcg
     1021 gcccggagcc cgattttcgc gagagaggtg gtcgttgccc cgcttgatcc ggcgcacgaa
     1081 cggacgttct ataccgccca cggcaactgg tttgcgaagt tggcatcggc ggtgagtctc
     1141 gtatttttgg gtctggcggt ttttcggaag aaatgatgcg gattttgcct gaactggaaa
     1201 aacagatgct ccggtgcatg ctgggtgcgg tcgtgattct cgcgccgttc gctgaaagtt
     1261 ttgcgcagac ctggctcaac ttcaccaata agaattcaat tgtggacgtg gcggccgggc
     1321 cgggcgtggt ctggtgcgcc accaccggcg gtgcgctccg ctttgatctc gcaacggatc
     1381 ggatcgacca gttcacgacg gcggaaggcc tgggggggac gtcacttttg tcggtcgcgg
     1441 tcgataccag cggcacggtc tggttcgggg ggaacaacgg cacgctcacg aaatacgaag
     1501 cgcgcaccaa ccgctggacg gttttccctt tcattgaccg ggataaccgc aaactcaaac
     1561 tgaaggacat cttcatcgac gggaacaagc tctggatggc caccgacgtc ggcgtgatac
     1621 tgttcgatgc ggtgcgcaac ggcggcgaga tccgggagac ctaccgccgg ctgggtacgc
     1681 tccagcagga agcggtgaat gcggtattgg tcaaagacgg gcggatctgg gcggcggcgg
     1741 ttggcggggt ggtctgggcc tttacgtcgg atcccaatct cttagatccg gcccgctggc
     1801 acaccttcac caaaacgacc ggcgacagtc tgcaggggga tcaataccag gcgctggccg
     1861 ctttcgatag cacggtcttt gtcggctcgg aggcggggct gttccgtctg gttgaaatgc
     1921 cggcggatac tttctggcag gtgctccccg tggcctccga tttgattcgg gccttgcacg
     1981 ctgccgggga ttccctcttt ttttccggcc gctttggctt gggcgtagtt acccggccag
     2041 gacttacggt cagtttcctc aacagcgacc ctcttggaac ccggattttc cccggtctgg
     2101 caatggctac cgacgggcgg ctcttcatcg ccacgcaagg gaaggggttg ggcatttacc
     2161 gcaatcagac ctaccagcgg cgggtgatcg acgggccgcc ggagaatttt gtcgtggatg
     2221 tggcgttgga ccgccgcggc cgcgtctgga ttgccaactg gaattccggt tttgcgatgt
     2281 acgatggcac ctggcatacc tttgataccc tgcccgttgc ccctttgggc tgggccgtga
     2341 ccgtcgatct ccaggataac gtctggtgct gttcctgggg cggtggaatt tatgaattca
     2401 ccagcgacgg acggtttcgg gcctttacgt ccgcgaattc gccggtgaga ggtatagcca
     2461 gtacatcagc gtacaccgtg tcgaccgacg cggtggttga ctttcagggc aatgtgtggt
     2521 tcaccaatcc gtatgcgtac gattcgaccg cgcttctggc tttccgcccc gattcgagct
     2581 gggaggtctt ttacaaaaat gcgcgcgacg ggctggtctc caacgacatc gtgatgctct
     2641 acccgcagga tgaccacatc tggatcgggt acaaaagcgc cggaacagca gcgggggtgg
     2701 gtgattttaa ttttaacggg acgcttggca accatgctga cgacattctc gtgttctaca
     2761 accaagagcg ggagaaactc ttaagtaaca cggtgacggc gattgccatc gaccggaccg
     2821 gcgttctctg ggtcggcacg accgccgggc tccagcgctt cgatcccgat ttgcggcgtt
     2881 tccttggtgt acagatgccg tcccctttag gaccgcaggt gaaagccatc accatcgacg
     2941 cgcgcaataa taagtggatt ggcacctcca ccggcttagg gttgatcacg cctgacggac
     3001 aattccgacc gcccattctt tccaccaata gcggtctggt gagcgatgcg gtggcggaaa
     3061 ttacccttga ccccgttacg ggcgacctct ggatcgccgc cgggtcacct ttctccggcg
     3121 aagaaggcgg gctctcgcgc ttccgcaccg gattcggtgg cacggtttcc gacctcaaac
     3181 aggtggaagc c
//
LOCUS       DBNGZA010000375         2179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1664569, whole genome shotgun sequence.
ACCESSION   DBNGZA010000375 DBNGZA010000000
VERSION     DBNGZA010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1664569"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1377)
                     /locus_tag="AC16FU_08195"
     CDS             complement(142..1377)
                     /locus_tag="AC16FU_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994386.1"
                     /translation="MQKRVNWFLCIFSVTLFCALAPTLSGWAWRHPAYVANRPPTPFS
                     LLLPPNGDTIRTSRPTFLWQSSSDPDPGDQVSYVLQITQDSTFALVPITVPVLGGDTA
                     FTPPVPLFVNNFEIWFWRVIAVDNLGEQTLSRETFQFTVLVSPDTTLLPAKIVFKPER
                     WDVRQLGAGGDDGDDDEGESEEESGAKIKCYIGNLPGHDVHEIDVATILLGDVVRVVQ
                     DRKCKSDKILPLTATLAADTPDTCFDARIQPEREGFDGAVLRVRFDREAAFRTIPPGA
                     RDGDTVFISVTGELQDGTRFAGRGRVIVKGDFPTGVPEEREVPRLISSSGAYPNPFNP
                     STTIRFSLLRDDFVAVSIYNLLGQRVGLIPYGFAQRGHHRVVWDAKDDSGSDLPSGVY
                     FFFIVGQTGQAGGKMVLVR"
     gene            complement(1609..>2179)
                     /locus_tag="AC16FU_08200"
     CDS             complement(1609..>2179)
                     /locus_tag="AC16FU_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HIR2994387.1"
                     /translation="TALSVFRRLFAVRAVPVVRPLLAQEPLTNGLQAVSGEPEPVAVE
                     IKYRLPIDLARLRQAFTDGTQETNYFLSLRTAEIKMISYLSREEERDKILATRNEYLL
                     IAKKNPKDGYRDMEDFIAGLTGEALKERLRSAIQGKGAFRRFKDVLMGYPEERRQWFR
                     FSEERDFVRLRRWLGQEGIAVAEEQVAAQ"
BASE COUNT          471 a          650 c          618 g          440 t
ORIGIN      
        1 gataattcgg ggttacagtg ggggtaaaac ggacaaagta ccaaataatg gaggctctac
       61 gcgtaccttt ggattttctc tgtgtcctct gtgcctccgt ggtgagattt gccttgcctt
      121 tcccgacact tcaacactcc cttaccgcac caacaccatc ttcccccccg cctgaccggt
      181 ttgtcccaca atgaagaaga agtaaacccc ggaaggaagg tcgctgccgg agtcatcctt
      241 cgcatcccag accacgcggt ggtggccgcg ctgcgcgaaa ccgtagggaa tcaaccccac
      301 ccgctgcccc aggagattat aaatcgacac ggcgacgaaa tcgtcgcgca aaagcgaaaa
      361 gcggatggtg gtgctggggt tgaatggatt ggggtaagcg ccggaactgg aaatcaaccg
      421 cggcacttcc cgctcttcgg gcacgccggt ggggaagtcg cctttcacga tcacccggcc
      481 gcgtccggca aaacgagtgc cgtcctgcaa ttcgccggtt acggaaatga aaaccgtatc
      541 gccgtcccgc gccccgggag gaatggtgcg gaaagcggct tcccggtcga atcgcacgcg
      601 gagcaccgcg ccgtcaaacc cttcccgttc cggttgaatt cgcgcgtcaa aacaggtatc
      661 cggcgtatct gccgccagag tcgctgtcag cggcaatatc ttgtccgatt tgcacttccg
      721 atcctggacc acccgcacga catcgcccaa aaggatcgtc gcgacatcta tctcgtgcac
      781 atcatggccg ggcaggttgc cgatgtagca tttgattttg gctccacttt cctcttcgct
      841 ttcgccctca tcgtcgtctc cgtcatcacc accggctccc aactgtctga catcccagcg
      901 ctccggtttg aaaacaattt tggcgggaag gagcgtcgtg tccgggctga ccaggacggt
      961 gaactgaaac gtctcccggg agagcgtctg ctcccccagg ttatcgacgg cgatcacccg
     1021 ccagaaccag atctcgaagt tattgacaaa aagcggtacg ggcggggtga aggcggtgtc
     1081 gccgcccaga accggcacgg tgatcggaac gagagcaaac gtgctgtcct gggtaatctg
     1141 gaggacgtag ctgacttgat caccaggatc ggggtccgac gacgactgcc agaggaacgt
     1201 cggccgactg gtgcggatag tgtcgccatt cggaggtaaa agaagcgaaa agggagtcgg
     1261 tggccgattg gcaacatagg cgggatgccg ccaggcccag ccggagagcg tcggtgccag
     1321 agcgcaaaag agcgtgaccg agaaaatgca caaaaaccaa ttgacccttt tctgcatacc
     1381 aatcacatct ccgccggtgc ccgtgcaccg gctttctccc agcacttcct tgcttggaaa
     1441 cctgagctcc gctttccctt agaggtcaga gagaaggggg aaagcgatct acagatacgc
     1501 tatctcctaa ggaattgcaa ccagtctgcc gggcagagaa aaatctaacc cattgtggca
     1561 taagaggtta cccattggtg ccgggcgtgc gggcgaaata ctgttcagtt attgagcagc
     1621 cacttgctct tcggcaaccg caattccttc ctgacccagc cagcggcgca gccgcacgaa
     1681 atcccgctct tcggaaaagc ggaaccactg gcgccgctcc tccggataac ccatcaagac
     1741 gtctttgaac cgccggaacg cccctttgcc ttggatggcg gagcgcagcc tttctttcag
     1801 cgcctcaccg gtgaggccgg cgatgaaatc ttccatgtcc cggtagccgt ccttggggtt
     1861 tttctttgcg atcagcagat attcattccg ggtggccagg attttgtcgc gctcttcttc
     1921 ccgggaaagg tagctgatca ttttaatttc ggcggttctc agcgacaaaa agtagttggt
     1981 ttcctgcgtc ccgtcggtga aggcctgccg gagtcgggcg agatcaatcg gcagtcgata
     2041 tttgatctcc acggccaccg gctcgggctc accggacacg gcctgtaacc cgttcgtgag
     2101 aggttcctgc gctaaaagtg gtcgtacgac cggtaccgcc cgcaccgcga acagacggcg
     2161 gaaaacactg agggcggtc
//
LOCUS       DBNGZA010000376         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1664837, whole genome shotgun sequence.
ACCESSION   DBNGZA010000376 DBNGZA010000000
VERSION     DBNGZA010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1664837"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..108
                     /gene="ffs"
                     /locus_tag="AC16FU_08205"
     ncRNA           8..108
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16FU_08205"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            234..1937
                     /gene="dnaX"
                     /locus_tag="AC16FU_08210"
     CDS             234..1937
                     /gene="dnaX"
                     /locus_tag="AC16FU_08210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR2994388.1"
                     /translation="MAYVVFARKYRPQTFAEVVAQEHVTQTLVNALSSGKISHAYLFS
                     GPRGTGKTTTARILAKALNCVNGPTPEPCNTCGFCQEITSGQSLDVVEIDAASNSGVD
                     DVRTLRENIRYTPAGGKYRVYIIDEVHMLSGAAFNALLKTLEEPPVHSVFVLATTELH
                     KVPATIVSRCQRFDFRRIPFERLSTALTDIAVKEHLAIDADAVALVAKKAEGSLRDGL
                     SLLDQLVAFAGERITRSEVEATLGLIAGEVLFEITDAIAARDEKQVLSVFQKQADAGA
                     DLKQFLDDLLEHFRNLLIARTAGDKAELFALATAERERYVTAAQTFAEGDLLRMLSSL
                     GALKTEFDKLSQPRIFLELELVKLARLDRTVEIANLIRRLEGGSMGGRHPTNAPLELL
                     LGETTEQNPPRRSGSPTANTSVPVKTSSALEKEVATAIPVASVKNFILDDIRRGCPTF
                     VEKLRAEKPTLAAILYEGKATLVEGSIVTVEVPNGQKFHLQMLEKRENLRLVEEILST
                     ACGIPPEAGLRVKISLAEKAAAEVSEKPSAGKRGEEIPEVKQLIEKFDAEVIDRRPVR
                     K"
     gene            1971..2282
                     /locus_tag="AC16FU_08215"
     CDS             1971..2282
                     /locus_tag="AC16FU_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR2994389.1"
                     /translation="MKGLGDIMKMAQNMQAKMETVQQELAVVRVEGTAGGGMVRVVAN
                     GKQEIMEVKIDPEVFNANDREMLEDMILAASNQAHQRAQELAAEKMAQLTGGIKIPGL
                     F"
     gene            2321..2914
                     /gene="recR"
                     /locus_tag="AC16FU_08220"
     CDS             2321..2914
                     /gene="recR"
                     /locus_tag="AC16FU_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR2994390.1"
                     /translation="MPTSTALENLITRLTKLPGIGRKSAQRVAFYLLKRPKEEAFLLA
                     EALRDVKEKVRTCSVCANITEDDPCPICRDPGRDRSTICVVEEINDVAAFDRTGEYRG
                     LFHVLGGALSPLDGIGPDELKIKELLTRISGGVKEVIVATNPNVEGEATAVYLAKVLK
                     PLGIKVSRIARGVPVGGALEFADQVTLTRSLEGRLEL"
     gene            3256..3459
                     /locus_tag="AC16FU_08225"
     CDS             3256..3459
                     /locus_tag="AC16FU_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2994391.1"
                     /translation="MPVGKVKWFNDQKGFGFIAQNDGGPDVFVHFSAIQDEGFKTLRE
                     GDEVQFEIVKGPKGPQAANVVKP"
     gene            3600..>4207
                     /gene="secA"
                     /locus_tag="AC16FU_08230"
     CDS             3600..>4207
                     /gene="secA"
                     /locus_tag="AC16FU_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR2994392.1"
                     /translation="MFPALITKLFGSKHERDVKKLRPLVAQMNEIYATLQDLTDEQLA
                     AKTPEFKDRLAQGETLDDLLPEAFAVVKEACRRLCGKSWPVCGIEVTWNMVPFDVQLI
                     GAMILHQGKIAEMATGEGKTLAATMPLYLNALEGEGTHLVTVNDYLARRDAEWMGEVY
                     KLLGLSVGCIQNEMDPAERRTQYNCDITYGTNNEFGFDYLRD"
BASE COUNT          988 a         1123 c         1292 g          804 t
ORIGIN      
        1 cgttgacggt caggtcctgc gcaacacgga ggcgcccaac cccgccaggc ccggaaggga
       61 gcaacggtaa gcgtttttcc gggtgtgccg cagggtcgcc gggcccgagc cggtcaccgg
      121 atacgcaaac tgcggcatag atcgagggtg ggtgcacgag cgttaaatag attcacagcc
      181 cttcgacgtt gctcagggtg gtgagtctgc cgaaccacag attataaact actatggcct
      241 acgtcgtttt cgcccgcaag taccgacccc agaccttcgc cgaggtggtg gcgcaagagc
      301 acgtcaccca gaccctggtt aatgcgcttt cctcgggcaa gatttcgcac gcttacctct
      361 tttccgggcc gcgcggcacc ggcaagacta ccaccgcgcg gattctggcc aaagcgctca
      421 actgcgtgaa cggcccgact ccggaaccgt gcaatacctg cgggttctgc caggaaatta
      481 cctccggcca gagtctggat gttgtcgaaa ttgatgcagc ctcaaattcg ggagtggacg
      541 acgtgcggac gttgcgcgag aatatccgct acacgccggc cggcgggaaa taccgggtct
      601 acatcattga cgaagtccat atgttgtccg gcgctgcttt caatgcgctc ttgaaaacgt
      661 tagaggagcc ccctgtgcac tcggtcttcg ttctggcaac aaccgaactg cacaaagtcc
      721 cggcgaccat tgtctcacgc tgtcagaggt ttgatttccg gcggattccg ttcgagcgac
      781 tctccacagc tttgaccgac atcgctgtca aggagcacct tgcaattgac gccgacgcgg
      841 tggctttagt tgccaagaaa gccgaaggaa gtttgcgcga cgggctttcg cttctggatc
      901 agctggtggc ttttgccggt gaacggatca caagaagcga agtcgaggcc acgctggggc
      961 tgattgcagg agaggtcctt ttcgagatta ccgacgccat cgctgccaga gatgaaaagc
     1021 aggtgctgtc ggtgtttcaa aagcaggccg atgccggggc cgacctcaaa caatttctgg
     1081 acgatttgct agagcatttc cgcaacctgc tcattgcccg cacggcaggc gacaaggccg
     1141 agttattcgc gctggcaacg gccgagcgcg agcgttacgt cactgccgct caaacttttg
     1201 ccgaaggcga ccttttgcgg atgctttcct ctttaggtgc gctcaaaacc gagtttgaca
     1261 aactctccca gccccggatt ttcttagaac tggaattagt caagttagcg cggctcgacc
     1321 ggacggtgga gattgcgaat ctgatccgca gattagaagg cgggtcgatg ggcggtcgcc
     1381 atccaaccaa tgcgccgttg gaactgctgt tgggagaaac gaccgagcaa aatccgccgc
     1441 gcagaagcgg ttctccaacg gccaacacgt cggtaccagt gaaaaccagt tccgccctgg
     1501 agaaagaggt tgcaacggcg atcccggttg caagcgtgaa gaattttatc cttgacgaca
     1561 tccggcgggg ctgcccgaca ttcgtggaaa agttacgcgc agagaaaccg actctggcgg
     1621 cgattctgta cgaggggaag gcgacactgg tggaaggaag catcgtgacc gtggaggttc
     1681 ccaacggcca aaagttccac ctccagatgt tggagaagcg ggaaaacctg cggctggtgg
     1741 aagagattct ctccaccgcc tgcggcattc cgcctgaggc cgggctccgg gtcaaaatct
     1801 ctctggccga aaaggcggcg gcggaggtga gcgaaaagcc atcagcaggg aagcggggcg
     1861 aagaaatccc ggaagtgaag cagttgatcg aaaaattcga cgcggaagta attgaccggc
     1921 ggccggtacg caaatagcgg tcgcccgcaa ctctctcaag gagcagtccg gtgaaaggtc
     1981 tcggcgacat aatgaagatg gcccagaaca tgcaggccaa gatggaaacg gtgcagcagg
     2041 aattagcggt tgtgcgggtg gagggcactg ccggcggggg gatggtgcgg gtggtggcta
     2101 acggcaaaca ggaaattatg gaagtgaaaa tcgacccgga agtgttcaac gccaacgacc
     2161 gggaaatgct cgaggatatg attctggcgg cctccaacca ggcgcaccag cgggcgcagg
     2221 aattagccgc cgagaagatg gcgcagttga ccggcggcat caaaataccg gggctattct
     2281 aaaagacagt agccagggac cagtagccag taaaagcaaa atgcctactt ccactgctct
     2341 tgaaaatctc atcacccgtt taacgaaact gccgggcatc gggcgcaaga gcgcccagcg
     2401 ggtcgccttt tacctcctca aacggccgaa agaagaagcg ttccttctgg cggaggcgct
     2461 ccgcgacgta aaagagaagg tgcgcacctg ctcggtctgc gccaacatca ccgaagacga
     2521 cccctgcccc atctgccggg accccggacg tgaccggagc accatttgcg tcgtcgaaga
     2581 aatcaatgac gtagccgcct ttgaccgtac cggcgagtac cggggtctgt ttcacgttct
     2641 gggcggcgcg ctctcgcctc tggatggcat tggcccggat gagctgaaga ttaaagagct
     2701 tttgacccgg atttcgggag gcgtcaaaga ggtcatcgtc gccactaatc cgaacgtaga
     2761 gggtgaggcc acggcagtct acctggccaa ggtgctcaag cccctgggga tcaaggtgag
     2821 ccgcatcgcg cggggggtgc cggtgggcgg ggcgctggag ttcgccgatc aggtgacgct
     2881 tacccggtcg ctggaggggc ggctggagct ctaatcgggg aagccgggcg gtcaggcggc
     2941 caggggggtc tcaaggaaca aagggcttct gccgataaat ttaaatggtg ccaccggcaa
     3001 aaaaggactt gacaaattcg gcggcagcac gtctataggg ttaagaacag gttggttaca
     3061 ggttgaagga agaggaaaaa gaacaaactg caaaaccgta aaaccccgcc gtgggcgggg
     3121 tggtacggct tttttgtaag gccctcccaa attgcaatcc agcacacatc gccacagcat
     3181 atcaagtttg agcgtcccgc agacggcggg atgcattggt gtttcactta taaccgtaaa
     3241 ggaggtgagc gatagatgcc agtaggaaaa gtgaagtggt tcaacgatca gaaggggttc
     3301 gggttcatag cccagaacga cggtggtccc gatgtctttg tgcacttttc ggcgatccag
     3361 gatgagggct tcaagactct gagagaaggg gacgaggtcc aattcgaaat cgtcaagggt
     3421 cccaaaggcc cccaagcggc gaacgtggtg aagccgtaac ttcacctgtt cgtccaagca
     3481 aaaccctgga taagcgattg tctggggttt tttatttctt ttttttaaga ctccaacttt
     3541 ttgtccgtat tccgagtgat tatattagac ggttaacgta aggatccaaa aggaagacga
     3601 tgtttccagc gctgataaca aaactattcg gctccaagca cgagcgggac gtcaagaaac
     3661 tgcggccgct cgtggcgcag atgaacgaaa tttacgccac gctgcaggac ttgaccgacg
     3721 agcagttggc cgccaagacg ccggagttca aggatcgtct ggctcaaggg gagaccttag
     3781 acgacctctt gccggaggcc tttgcggtgg tcaaggaggc ctgccggcgg ctctgcggga
     3841 agtcgtggcc ggtctgcggg attgaggtca cctggaacat ggtgccgttc gacgtgcaat
     3901 tgatcggcgc catgatactg catcagggga aaatcgccga aatggcgacc ggtgaaggga
     3961 aaactttggc ggccaccatg ccgctctacc tgaacgcctt agagggcgaa ggcactcact
     4021 tagtcaccgt aaacgactat ttggcccggc gtgacgccga atggatgggg gaggtctata
     4081 agctcctcgg gctctcagtc ggctgcattc agaacgagat ggacccggct gaacggcgca
     4141 cccagtacaa ctgcgacatc acctacggca cgaataacga attcggcttc gactacctgc
     4201 gcgacaa
//
LOCUS       DBNGZA010000377         4293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2194133, whole genome shotgun sequence.
ACCESSION   DBNGZA010000377 DBNGZA010000000
VERSION     DBNGZA010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2194133"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1536
                     /locus_tag="AC16FU_08235"
     CDS             94..1536
                     /locus_tag="AC16FU_08235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2994393.1"
                     /translation="MKEALLEGIAFLKKKKVDYADIRWVKNEQEMILVRNGNVEGLSR
                     DVDQGFGVRVLAGGAWGFAASAVLTPAELKRVAQKALDVAKASALTLKQKVKLAEEQP
                     HVDRYQTPVKRDPLAVSIDEKIGLLIDLTTGLQKEPKVKVAEANLDFRKISKWFVSTE
                     GAQIEQIITQSGAGMVATAVAGAEVQRRSYPNSHRGDFANAGWEFIEQMRMRDQIERV
                     RREAAALLSAPACPATETTVVISAPQMALQVHESCGHPIELDRVLGTEISLAGGSFMT
                     TDKFGKLRYGSPKVNIVGDATIPEGLGSFGYDDEGVKAQRFDIIRTGLFCGYLTSRET
                     APVLNQKSNGTMRADGWNRIPLIRMTNINLEPGEGTLDDLIADTREGLFIDMNKAWSI
                     DDKRLNFQFGCEVAWEIKDGKLGQMYRNPTYTGITPEFWNSCDAVCNRNHWKLWGVPN
                     CGKGEPMQVARVAHGAAPARFRRVRVGVSA"
     gene            1533..2885
                     /locus_tag="AC16FU_08240"
     CDS             1533..2885
                     /locus_tag="AC16FU_08240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2994394.1"
                     /translation="MIGKDRLLSTLQKVLKKAGADQAEAVFLGGESGLTRYANSTIHQ
                     NVTERNHKIYFRVALGKKIGVAATNSMTPDDLQLALGNALTIAKQQRANPDFTSFPAK
                     AKYPKVRTHFKATGAFTPKERALAVKKAIEKAARHNLQIYGSFATGEGELAVANSLGV
                     AAYQPLSNAALNLIAMGDSSSGYADGLSRDVREVNIDSIAETAIQKCLDSSNPKDFPP
                     GTYEVILEPAAVANLMEWMSFVSFGSKAFQEQTSCLAGKIGQKIMADSVTIYDDGGDE
                     GGAALPFDFEGVPKKKVVFVDRGVAKGVVYDTLAANREKKKSTGHALTPDSASEGAIP
                     VNIFIKGSTSSLKKMIANCERGLLVTRFHYINGLFDPPNAAMTGMTRDGTFLVEGGKI
                     KHGIKNLRFTESMVKAFSQVAEISRERNRVAAWWESIGCITTPAVRITRFAFTGKTDF
                     "
     gene            3044..>4293
                     /locus_tag="AC16FU_08245"
     CDS             3044..>4293
                     /locus_tag="AC16FU_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994395.1"
                     /translation="MNHRTELRRGFKILLVISAVAGNVAAQERTVVSISHFKPGEIQA
                     AVLTVKEPAKLRIRALGAAWKEDGDLYAYGWILDLGSRKVVWEMDRDNTESVRRTHVR
                     EFDGTVKLNAGLYKVYYFAGRPFFFGGKTDINSWDDFWNLLGNIFSGERYDEKNWEKV
                     GSKFFLEAAAAGAVTLADGDPYEKRAIVALTKPGNSRSLSAGFSVGAEVKVKIYASGE
                     YDDNTGTLADYGWIIDAGTRKRVWEMDRHHVDPAGGAEKNVQFTGEIGLPRGNYVATF
                     ITDDSHAYGKWNALPPSDPDNYGLVILPAAEGERKLFAPYTDTYREPVVIQLVEVGDD
                     DYVSAGFTLDRGTELHIYALGEYDQGEDQLADYGWIEDAGTGKKVWEMTPDNVVPAGG
                     AEKNKKFDDVITLPRGSYVVNYLS"
BASE COUNT          981 a         1187 c         1318 g          807 t
ORIGIN      
        1 caggatagcg gaggagtcgt ttgacagcgg cggatggggc acataagtta cgccacgttt
       61 tgtctaagta attttcttta ccaaggaggt aaggtgaaag aagcgctttt agagggaatt
      121 gcttttctga aaaaaaagaa ggttgattac gccgacatcc gctgggtgaa aaacgagcag
      181 gagatgattt tagtccgcaa cggtaacgta gaggggctct cccgcgacgt agatcagggt
      241 tttggcgtgc gggttttagc cggcggggcc tgggggttcg cggccagcgc ggtcctcact
      301 cccgccgagt taaagcgtgt ggcgcagaag gcgttagacg tcgccaaagc cagcgcactg
      361 acgcttaaac aaaaagtgaa actggccgaa gagcaacccc acgttgaccg ctaccagact
      421 ccagtaaaac gcgatccctt ggccgtcagc attgatgaga agattggtct tttgatagat
      481 cttaccaccg gtttgcaaaa ggagccgaaa gtcaaagtcg ccgaagcgaa tttggacttc
      541 cgcaaaatca gcaagtggtt cgtctccacc gagggggcgc agatcgagca gatcattacc
      601 cagtcggggg cggggatggt ggcgacggcg gtcgcggggg ccgaagtgca gcggcgctcc
      661 tacccgaatt cccaccgggg cgatttcgcc aacgcagggt gggagttcat cgaacagatg
      721 cgaatgcgcg atcagattga acgggtgcgc cgcgaagcgg cggcgctttt gtccgcgcct
      781 gcctgtccgg ccaccgaaac aacggtcgtc atctccgcgc cgcagatggc gctgcaggtg
      841 cacgagtcgt gcggtcaccc gattgaactc gaccgggtgc tgggtaccga aatcagttta
      901 gccggcggca gtttcatgac tacagacaag ttcggcaaac tgcgctacgg ttcgccgaag
      961 gtgaacatcg tcggtgacgc gaccatcccc gagggactgg gaagtttcgg ctatgacgac
     1021 gaaggagtga aagcgcagcg cttcgacatc atccgcaccg gattattctg cggctacctc
     1081 acctcgcggg agaccgcacc ggtgctcaat cagaaatcga acggcaccat gcgggccgac
     1141 ggttggaacc ggatcccgct catccggatg accaacatca acctcgagcc gggcgaaggg
     1201 acgttggacg acttgatcgc cgacaccaga gaaggactct tcatcgacat gaacaaggct
     1261 tggtcgattg acgacaagcg gctgaacttc cagttcgggt gtgaagtggc ctgggaaatc
     1321 aaagacggga aattgggaca gatgtacagg aacccgacct acaccggcat tactccggag
     1381 ttctggaatt cctgcgatgc ggtctgtaac cgcaatcact ggaagctgtg gggtgtgccg
     1441 aactgcggca aaggagagcc gatgcaggtg gcgcgggtgg cgcatggggc ggcaccggcc
     1501 cgcttccgca gggtgcgggt gggggtgagc gcatgatcgg caaagaccgc ctcctttcca
     1561 cgttacagaa agttctcaag aaggccgggg ccgaccaggc ggaagcagtt ttcttaggcg
     1621 gtgagagcgg cctcacccgc tacgccaact ccacgattca ccagaacgtc accgaacgca
     1681 atcacaagat ctacttccgg gtagcgctgg ggaagaagat cggcgtcgcc gcgaccaact
     1741 caatgacccc tgacgatttg caactggcac tcggcaacgc gctcaccatt gccaaacagc
     1801 agcgagccaa tcccgatttc acctcctttc ctgcgaaagc gaagtatcca aaagtccgca
     1861 cccatttcaa agcaaccggc gcttttactc ccaaggagcg tgcgctggcg gtaaagaagg
     1921 caatcgaaaa agcggcccgg cataacctgc aaatctacgg ttcctttgcc accggcgaag
     1981 gggagctggc ggtagcgaac tcgctcggag tggcagccta ccagccgctc tccaacgccg
     2041 cgctgaatct catcgccatg ggggactcca gttccggtta cgccgacgga ctttcccgcg
     2101 atgtgcggga ggtcaacatc gattcgattg ccgaaaccgc catccagaag tgcctcgata
     2161 gcagcaaccc gaaagatttt ccgccgggga cctacgaggt gattctggag ccggcggcag
     2221 tggccaattt gatggagtgg atgagttttg tttccttcgg ctccaaggcg tttcaggaac
     2281 agaccagctg cctggcggga aaaatcggcc agaagattat ggccgacagc gttacgattt
     2341 acgacgacgg cggcgacgag ggcggcgcgg cgctgccgtt tgatttcgag ggcgtgccga
     2401 agaaaaaagt cgtcttcgtg gaccgcggtg tcgccaaagg agtggtctac gacacgctgg
     2461 cggctaaccg cgagaagaaa aaatccaccg gccacgccct gacgccggac agtgccagtg
     2521 aaggggctat tccggtgaat atctttatta aaggcagtac ctcttcgctc aaaaagatga
     2581 tcgccaactg tgagcgggga ctgctggtga cgcgcttcca ctacatcaac ggcctttttg
     2641 atccaccgaa tgccgccatg acgggtatga cccgtgacgg aacgtttctg gtcgagggcg
     2701 gaaaaatcaa gcacgggatc aaaaacctgc gcttcaccga gagcatggtc aaggccttct
     2761 cgcaggtggc ggagatttcc cgcgagcgga accgggtggc tgcctggtgg gaatcgatcg
     2821 gctgcatcac caccccggcg gtacgcatta cccgatttgc ctttacggga aagaccgact
     2881 tctaacagct cttcccggca accccaagtc cctatcggcc gggtggagac ttggtcttct
     2941 cgggtaatgt acggccgcac aaaaaattgc aacctttttc ctctcctctc gtaaagaaaa
     3001 ccaggcgcaa gatgtccttg ggccgatggg tgactttatc tctatgaatc accggaccga
     3061 attgcggcgg ggtttcaaaa tcctcctggt gatctcggct gtggccggca acgtggcggc
     3121 gcaggagcgg accgtggttt ctatttccca tttcaaaccg ggggaaattc aagcggcggt
     3181 gctcacggtg aaggagcccg ccaagttgcg cattcgcgcc ttgggtgcgg cctggaagga
     3241 agacggcgac ctgtacgcct acgggtggat tctcgatctc gggtcccgaa aggtggtctg
     3301 ggaaatggac cgggacaata ctgaatcggt gcgccgtact cacgtgcgcg aatttgacgg
     3361 caccgtgaag ctgaatgccg ggctgtacaa agtctactac tttgcgggtc ggccgttctt
     3421 tttcgggggc aagacggata tcaactcctg ggacgacttc tggaatcttc tgggaaacat
     3481 tttttccgga gaacgatacg acgagaagaa ctgggagaaa gtcggaagca aattctttct
     3541 tgaagcagcg gctgccggcg ccgttacact ggccgacggc gatccgtacg agaagcgggc
     3601 gatcgtcgcg ctgactaagc cgggcaattc ccgttccctt tccgccggat tttcggtggg
     3661 cgctgaagtg aaggtaaaaa tttacgccag cggcgagtac gacgataaca ccggaacatt
     3721 ggccgattac ggctggatca ttgacgccgg cacgcgcaaa agggtctggg aaatggaccg
     3781 gcatcacgtc gatccggcgg gcggggccga gaaaaacgtg caattcaccg gcgagattgg
     3841 cctgcccagg gggaactacg tcgccacctt catcaccgac gacagccacg catacggcaa
     3901 gtggaacgcg ctcccgccct ccgatcccga caactacggc cttgtcatcc tccccgcagc
     3961 cgaaggtgag cgaaagctct ttgccccgta caccgacacc taccgggagc cggtggtcat
     4021 ccaactggtg gaggtgggtg acgatgatta cgtttcggcc gggttcacgc ttgatcgagg
     4081 caccgaactc cacatctacg ccttgggcga gtatgaccag ggcgaggacc aactggccga
     4141 ttacggctgg attgaggacg cgggcactgg aaagaaagtc tgggagatga ccccggacaa
     4201 cgtcgtgccg gccggcgggg cggagaagaa caaaaaattc gacgatgtca tcacgctccc
     4261 gcgcggcagt tacgtggtca actatctctc cga
//
LOCUS       DBNGZA010000378         6181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1028375, whole genome shotgun sequence.
ACCESSION   DBNGZA010000378 DBNGZA010000000
VERSION     DBNGZA010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1028375"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1276)
                     /locus_tag="AC16FU_08250"
     CDS             complement(65..1276)
                     /locus_tag="AC16FU_08250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR2994396.1"
                     /translation="MDLFEKCRRFTEARKVMAAGYYPYFRPISSSLGTEVVVKGQKMI
                     MIGSNNYLGLTNHPKVKAAAEAAVEKYGSGCTGSRFLNGTLDIHEELESRLAAFMRKE
                     AGLVFSTGFQTNLGTISAIVQKGEYLIIDRSVHASIVDGCRLAFGKTIKYRHNDMDDL
                     KRVLASLGKGAGKLIVVDGVFSMEGDIAKLPEICDLARRYNARTLVDDAHSIGVLGPG
                     GRGTAEHFDLEDQIDIIMGTFSKSFASLGGFIVAREEVIHYIKHFSRELIFSASMPPS
                     AVAACLAALDIIETEPQRRARLWHITRRVHGELRGLGFDIGVTETPIVPIYIGADMRT
                     FQFWKALTDAGVFSNPIVSPAVPKGRALIRTSYTATHTDEQIDKVLGLLAEVGQRFGI
                     LKPQPVPVGDA"
     gene            1396..2376
                     /locus_tag="AC16FU_08255"
     CDS             1396..2376
                     /locus_tag="AC16FU_08255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2994397.1"
                     /translation="MRALVTGATGFVGHHLAALLVARGYTVRVLLRPQSEHKIAQELS
                     VEPVFGGLADEASLIPAVAGCDLVFHCAGLVKAAHPEDFFAVNHEGTQHLLEAVLASR
                     QKLSRFVYVSSVAAGGPSLNGQPLDETVPVAPVSPYGRSKLAGERAVLGLKEKIPVTI
                     IRPPAVFGPRDKQMLFFFQMVKRGWIPRFSRDDRRLSLIYVGNLVQGIIQAAESPRAV
                     GEVFFLADGRTYVTSEVVHVIAAALRVKVKQVYVPGPVLYTLAAAAELWGNITGRPPL
                     LWRGRARDLSRYLWIVNIEKARRAFGYAPDVPLEESVRETCAWYQRAGWL"
     gene            2403..3572
                     /locus_tag="AC16FU_08260"
     CDS             2403..3572
                     /locus_tag="AC16FU_08260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR2994398.1"
                     /translation="MTERLATGMAKYRREFPFTRHTVFLNTAAFGPLPLRIKKMLDRF
                     DRTMLELDDWEYDEKTFHLLDGSRALAARLVNARSDQVGFSFNTSFGLNICAAGLPWH
                     PGDEVLLSGVDFPANIYTWSNLKRRGVAIRFLSRQGRDCIDPEQVEQSIAPRTKLLAL
                     SFVQYFNGYKNDLETLGEICRARNVWLVVDGTQGVGAEMLDVRKAQIDFLACGGQKWL
                     LSAPGTGFFYISDRLREVLEPIFFSWLGVDWGLDFSDLQKHDLRPFASARRFEIGSYP
                     ARLVRHFHAALELILEAGVARIHEHNHALLDRLLAYLADSPYRLKSPWSEERRSSIVS
                     FASRDNRRLLQFLRKRKISVALRERGIRVSPHFFNNAADMDSLIGALKQFQAKSR"
     gene            3614..4189
                     /locus_tag="AC16FU_08265"
     CDS             3614..4189
                     /locus_tag="AC16FU_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994399.1"
                     /translation="MAKKYLLALLGLGMVFATGCARRVVVVERQTPPPPAARHRGQAV
                     ASQEHLENGKRMFHRGRYRQAAHQFEIAIAEDPNNWEAHWWLGLTLREMRDYDDCLDH
                     FRVAIRLHDDPDWVARVRVDVGLVWELRGDRDKALVEYDLALRARPGYADAIKARERL
                     KERREALKEREDDKDKGHRGNKGKRRSERDD"
     gene            complement(4221..4880)
                     /locus_tag="AC16FU_08270"
     CDS             complement(4221..4880)
                     /locus_tag="AC16FU_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994400.1"
                     /translation="MQILEITFGRFPEIIRLNPAFRTGAKQVRVLLRVESNGVDYRSR
                     FAAVFSGLTRWFPTLAHHDCCQWGDHAPQIQTDGIPLKTTGGLADVAHLVEHLMVDLH
                     CRVGGMRRCSGVTCGYRKPADRFDLFVECPEHRLGRFSAGFATFLVRQLFQRRKVSRG
                     YRRVLALARYLLAHPRAPLDRQKLSRTMGWTQSQTRFAFRKLAEFQFLENGSTNGGQK
                     K"
     gene            complement(5017..5790)
                     /locus_tag="AC16FU_08275"
     CDS             complement(5017..5790)
                     /locus_tag="AC16FU_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994401.1"
                     /translation="MVKYFATLLACAALFLAGCSQKQTEQTPEETPQMGQADQQNEPQ
                     AQPEKQTNTQASNKTMKKSSRTAGQQAPKAEEAKPVVLEVPAGMQLVVALSDSINSGK
                     NKTGEKFDATLAKPIVVNGQTVIEEGAPITGEIILARGSSGLKSTAELVLTLNEIEAH
                     GQAYHVTTTTVEAKEKSKTGRNVGMIGGGAAVGTAIGAIAGKKKGAIIGGVAGAAAGT
                     GAAVLTGKKNLLYPAGSELTFILQQPMKVTVKKSALAAH"
     gene            complement(5963..>6181)
                     /locus_tag="AC16FU_08280"
     CDS             complement(5963..>6181)
                     /locus_tag="AC16FU_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994402.1"
                     /translation="VCTPAFSPSENPVLATVAAWTPQSSCHATNISIWWLEWDFTLRY
                     SPNPYSPTITGGWFRLGARLFGIEARKE"
BASE COUNT         1214 a         1719 c         1892 g         1356 t
ORIGIN      
        1 agcccttctg ttttaatgga gaaagtgggc cgggagttgt ccggcggtcg cggcggctgg
       61 agaattatgc atctccaacg ggtaccggct gcggtttgag gatgccgaag cgctggccca
      121 cctccgccag gaggcccagc actttgtcga tctgctcgtc cgtgtgggtg gcggtgtaac
      181 tggtgcggat caaggcccgg cctttgggca cggcggggga aacgatcgga ttgctgaata
      241 cgcccgcatc tgttaacgcc ttccagaatt ggaacgtgcg catatcggca ccgatgtaaa
      301 tcggcacaat gggggtctcg gtcaccccga tgtcaaaccc caacccccgc agttcgccgt
      361 gtacccggcg ggtgatatgc cacagccggg cgcgcctctg gggctcggtc tcgattatgt
      421 ctaacgccgc caggcaggcc gccaccgcag agggcggcat gctggcagag aagatcaatt
      481 cccgtgagaa gtgtttgatg tagtggatga cttcctctct ggccacgatg aatccgccca
      541 gagaggcgaa cgacttgctg aaggtcccca tgatgatatc gatctggtct tccaggtcga
      601 agtgttcggc ggtgccccgg ccgcccgggc ccaaaacgcc aatcgagtgg gcgtcgtcca
      661 ccagggtacg ggcgttgtac ctgcgcgcca ggtcgcagat ctccggtagt ttggcgatgt
      721 cgccctccat gctgaagacg ccatcgacca cgatcaattt cccggcgcct ttgcccaaag
      781 aggccagcac ccgcttgagg tcgtccatat cgttgtgccg gtacttgatc gtcttgccga
      841 aggccagccg gcagccgtct acaatcgaag catggaccga gcggtcgata atgaggtact
      901 cgcccttttg gacgatggct gaaatagtgc cgaggttggt ctggaagccg gtggaaaaga
      961 ccagcccggc ctctttgcgc ataaaggcgg ccaggcggga ctctaattct tcgtgaatgt
     1021 ccagggtgcc gttcaaaaag cgtgaaccgg tgcagcccga accgtatttt tccaccgcgg
     1081 cctcggcggc ggctttgact ttgggatgat tggtaagccc caggtagtta ttggagccga
     1141 tcataatcat cttctggcct ttgaccacca cttcggtccc cagagaagag gaaataggcc
     1201 ggaagtaggg atagtagcct gcggccatga ctttgcgcgc ctcggtaaaa cggcggcatt
     1261 tttcaaataa gtccataaga ccttaggtat ttaggtgaaa ttgtgtacgc tggtctgaaa
     1321 ccccgccttt tcatctcgcc aaagggtaat atccgacgat ttgcctgtca agagcaatat
     1381 gcgttacttg agattgtgcg cgcgctggta acgggtgcca ccggatttgt gggacatcac
     1441 cttgcggctc tactggttgc ccgggggtac accgtgcggg tacttttgcg tccccaaagc
     1501 gagcataaaa tcgctcaaga actttcggtt gagccggttt ttggcggtct ggcggacgag
     1561 gccagtctca tcccggcggt tgccggctgt gatctggtat tccactgcgc cggactggtc
     1621 aaggccgctc atccggaaga tttctttgcc gtgaatcacg agggcacgca gcaccttttg
     1681 gaagcggtgc tcgcttcccg gcagaaactg tcacgcttcg tctacgtttc cagtgtcgcg
     1741 gccggcgggc cgagtttgaa cggccagccg ctggatgaga ccgttcctgt tgcgccggtt
     1801 tcgccctatg gcaggtcgaa attggccggg gaacgggcgg tcctggggct caaagaaaaa
     1861 attccggtga ccatcattcg ccccccggca gtgttcggac cacgggataa gcagatgctc
     1921 tttttcttcc agatggtgaa aagaggttgg attccccgct tcagccggga tgaccggcgg
     1981 ttaagtttga tctacgtggg aaatctcgtg caggggatta tccaggcggc ggaaagcccc
     2041 agggccgtgg gcgaagtttt ttttctggcg gacggccgga cgtatgttac cagcgaagtg
     2101 gttcacgtca ttgccgccgc cttgcgggtg aaggtgaagc aagtctacgt acccggaccg
     2161 gtactctata ctctggcggc ggctgccgaa ctgtggggga acataacggg aaggccgccg
     2221 ctattatggc gcgggcgggc ccgcgacctt tcgcgctatc tttggatagt aaacatagag
     2281 aaagcgcgcc gggcctttgg ctacgcgccg gacgttccct tagaggaaag tgttcgcgaa
     2341 acctgtgcgt ggtaccagag ggcgggatgg ttgtagagat caggtcaagt aagggaagga
     2401 gagtgacaga acggttggca accgggatgg cgaaataccg gagagaattt ccgttcacac
     2461 gacataccgt ttttctgaat actgcggctt ttggcccgct gccgctgcgg atcaaaaaga
     2521 tgctcgaccg cttcgaccgc accatgctcg agctcgatga ttgggagtac gacgaaaaga
     2581 cctttcattt actcgacggc tcccgcgctc tggccgcgcg tctcgtcaat gcccgttctg
     2641 accaggtagg cttcagtttt aacacttcct ttggcttgaa tatctgcgcc gccggcctgc
     2701 cctggcaccc gggggacgaa gttttgctct ccggcgttga ttttcccgcc aatatctata
     2761 cctggtccaa tctgaagcgg cggggagtcg ctatccggtt tttgtcccgc caagggcggg
     2821 actgcatcga tccggagcag gtcgaacaat caattgcccc ccgaacgaaa ttgctggcgt
     2881 taagttttgt ccagtatttc aacgggtaca aaaacgattt ggaaactttg ggagaaatct
     2941 gtcgggcccg gaacgtctgg ctggtcgttg acggcaccca gggggtgggc gccgaaatgc
     3001 tggacgtccg gaaggcgcag atcgatttcc tggcctgcgg cggccagaag tggctccttt
     3061 ccgctcccgg caccggtttt ttttacatct ccgaccgttt gagggaagtg ctggagccga
     3121 tctttttcag ctggctgggg gtggactggg gactcgactt ttccgacctc cagaagcatg
     3181 atcttcgccc cttcgcctca gcccggcgct ttgaaatcgg ctcttacccg gcgcggctgg
     3241 tgcggcattt ccacgcggct ttggaactga ttctggaagc cggagtcgcc cgcatccacg
     3301 aacacaacca cgccctgctg gaccgcctgc tggcctatct ggccgactcg ccctaccggc
     3361 tcaaaagtcc ctggtcggaa gagcgccgtt ccagcattgt ttcgtttgcg agtcgggaca
     3421 atcggcgtct gttgcagttc ctcaggaaaa ggaagatttc cgtggccctg cgagaaaggg
     3481 gcattcgcgt ttccccgcac ttctttaata atgcagccga catggatagc ctgattggag
     3541 cgcttaaaca atttcaggca aaaagccgat agtagggttg gtagtgatgc aaccgactca
     3601 caaggagggg aggatggcaa agaagtatct tttggccctt ttggggctgg gtatggtatt
     3661 cgccaccggc tgcgcccggc gggtggtagt ggtggagcgt caaacacccc cgccgccggc
     3721 agccaggcac cgggggcagg cggtcgcctc gcaggaacac ctggagaatg ggaagcggat
     3781 gttccaccgc ggccgctacc gccaggcggc gcaccagttc gaaatagcga ttgcggaaga
     3841 cccaaacaac tgggaagccc actggtggct gggtttgact ttgcgcgaaa tgcgcgacta
     3901 cgacgactgt ctggatcact tccgggttgc catccggctg cacgacgatc ccgactgggt
     3961 ggcccgggtg cgggtggacg tcggtctggt ctgggagctg cgcggcgacc gggataaggc
     4021 cttagttgag tacgacctgg cactgcgggc gcggcccggc tatgcggatg ccatcaaagc
     4081 cagggagcgg ctcaaagagc gccgggaagc cttgaaagag cgcgaggacg acaaggacaa
     4141 aggacaccgg ggtaacaagg ggaagcgtcg cagcgagcgc gacgattgag gccaaaaggc
     4201 cccgccttgg cggggccttt tcatttcttt tggccgccgt tggttgaccc gttctccaga
     4261 aattgaaatt ccgccaattt ccggaacgca aagcgggtct ggctttgggt ccagcccatt
     4321 gtgcgcgaga gcttttgccg gtcgagcggc gcccgcgggt gggccaagag gtagcgggct
     4381 aaagcgagca cgcgccggta gccccgcgaa acttttcggc gctggaagag ttgccgtacc
     4441 aaaaacgtcg cgaaaccggc gctgaaccgc cccagccggt gctccggaca ttccacaaaa
     4501 aggtcaaagc ggtcggccgg cttccggtag ccgcaggtga ccccgctgca cctccgcatc
     4561 ccgcccaccc ggcagtggag gtcaaccatc aggtgctcga ccaggtgggc gacatccgcc
     4621 agaccgccgg ttgtttttag cggtatgcca tcggtttgga tttgcggcgc gtgatcgccc
     4681 cactgacagc agtcgtggtg cgcgagagtc ggaaaccagc gggtcaagcc cgagaaaacc
     4741 gcagcgaagc ggctgcggta gtcaacgccg ttggattcta cccgtaaaag tacccgcacc
     4801 tgtttggcgc cggtgcggaa agcggggttg agccggataa tttccgggaa ccggccgaag
     4861 gtgatttcca gaatttgcat ggtcttttgt cggacaggcg tactcctttt tgtatcggcc
     4921 aatggagaaa gcaacttgac ggaaatagga aaaagcaggc aaaaaaatac ctccgatgct
     4981 gctctcatcg gaggtagaaa agccgtaatc tgttttttag tgggcggcca gggcgctctt
     5041 tttgaccgtc accttcatcg gctgctgcag gatgaaggtc aactcgctgc cggccgggta
     5101 gaggaggttc tttttgccgg tcaaaactgc ggcgccggtc ccggcggcgg cacctgccac
     5161 tccgccgata atggcacctt tctttttgcc ggcgatggca ccaattgccg ttcccactgc
     5221 ggctcccccg ccgatcatac cgacgttgcg gccggttttc gacttttctt tggcttccac
     5281 cgtggtggtg gtgacgtggt aggcctggcc gtgagcttcg atttcattca aggtcagcac
     5341 cagttcggcg gttgacttca gtccggatga accgcgcgcc aggataattt cgcccgtaat
     5401 cggcgcgccc tcttcgatca cggtctggcc gttgacaacg atcggcttgg ccagggtggc
     5461 gtcaaacttc tcgccggtct tgttcttgcc ggaattgatg gaatccgaca gcgccaccac
     5521 taactgcatt ccggcgggga cttccagcac caccggcttg gcttcttccg ccttgggcgc
     5581 ctgctgtccc gccgtgcggg acgacttctt catcgtcttg ttcgaggctt gcgtgttggt
     5641 ttgcttttcc ggctgcgctt gcggctcgtt ttgctgatcc gcctggccca tctggggcgt
     5701 ctcttccggc gtttgctcgg tctgcttctg cgagcagccg gccagaaaca gtgccgcgca
     5761 ggccagaagt gttgcgaagt atttcaccat attttccttc ctcctttcga tgtcctatat
     5821 gtcgtcacaa atagtacgaa tgtttagggc aaacgatagt acattgagcg agaagctgca
     5881 agcaatttat gttcagcaag cataagtctt gactcttgta gtgcagcgaa actttctacc
     5941 cgacaatttc tttcaatctt tcttactctt ttctagcttc aataccaaaa agccgtgcac
     6001 cgagtctaaa ccagccgcca gtaatggttg gtgagtaagg attaggagaa tagcgaaggg
     6061 taaagtccca ttccagccac caaattgata tgttggtggc atggcaagac gactgaggtg
     6121 tccacgctgc gacagtggca agtactggat tctccgaggg agaaaacgcc ggtgtgcaca
     6181 c
//
LOCUS       DBNGZA010000379         2728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_393182, whole genome shotgun sequence.
ACCESSION   DBNGZA010000379 DBNGZA010000000
VERSION     DBNGZA010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_393182"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="AC16FU_08285"
     CDS             <1..1200
                     /locus_tag="AC16FU_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2994403.1"
                     /translation="AANFFGFDARVMAMGGAALANATSGSATNYNPAALARIHRIELS
                     LGLSHQRSRNNTEAPPVAALALGNPVVPTERTINNTRFNSAWAVLPVPTYRGSLVFGV
                     SGVRAKNFDRAFERRTLDSVSAADFRRTFVSERESGGLYAWSVGGAVDMSPNVSLGAT
                     LILWTGTDAFASSGSLDSAAGGPPGTFRFADNIEDRYTGFTARVGALIQPNRMLTLGF
                     TVDSPSWFSIDEDKIFRQTDTTGQLVESPQPLFQYDLIHPFAFGAGAKVNIAGLTLAG
                     DLGFTDWSQMEYRNTPDARLQSDSLQQNYRDVLRINLGAEYTIPVTGVSLRAGFLSDP
                     LPFKSSVIQTNRKFFTFGAGFLVDQVLTLDAAVVLGDFGFNNYGNFFYTEKYNLTRVF
                     VTAAWRL"
     gene            complement(1398..2054)
                     /locus_tag="AC16FU_08290"
     CDS             complement(1398..2054)
                     /locus_tag="AC16FU_08290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2994404.1"
                     /translation="MYKASKRACDLFFATVALVLLSPFFVLAALAILFDSGRPVFFKQ
                     TRVGKDGVLFEMLKYRSMIKGAEQSGPRVTAPRDPRITRIGLILRKTKIDELPQLVNV
                     LKGEMSLVGPRPEVPRLASRYSAEQKKIFQVKPGLTSPGTLYYYRFQAHDHPEGVDLE
                     DYYLQKQLPEKLAYDFAYLNRPAIGKDLLLLLKTLWVIVTTPFVRPQAVESKGNIMPD
                     "
     gene            2243..2473
                     /locus_tag="AC16FU_08295"
     CDS             2243..2473
                     /locus_tag="AC16FU_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994405.1"
                     /translation="MGDLTIIAISMSVLALTWAVVRMNERVEDLQKKLRRISDQFNRD
                     TVGQRTPAAPRPMQAGSTYQGPMQPGQMQPVG"
BASE COUNT          575 a          789 c          783 g          581 t
ORIGIN      
        1 gcggccaact tcttcgggtt tgacgcccgg gtgatggcga tgggcggcgc ggccttggcc
       61 aacgccacct ccggctcggc gacgaattac aatcccgccg ccttagcccg catccaccga
      121 attgaacttt cgctgggctt gagccaccaa aggtcacgca ataacacaga ggccccccct
      181 gtggcggcat tagctttagg aaatcctgtc gtgcctaccg agcggactat caacaacacc
      241 cgcttcaact cggcctgggc ggtactgcca gtgccgacct accggggcag tctggtcttc
      301 ggcgtttcgg gagtgcgggc gaagaatttc gaccgggcct ttgagcgccg gaccctcgat
      361 tccgtttccg cggcagactt cagacgcact ttcgtgagcg aacgggaatc gggcgggctc
      421 tacgcctggt ccgttggcgg ggcagtggat atgtcgccca atgtctcttt gggggctacg
      481 ctcatcctct ggacgggcac cgatgccttc gcttcgtccg gctcccttga ctcagccgcc
      541 ggcggtcctc ccggcacgtt ccgctttgcc gacaacatcg aagaccgcta caccggtttc
      601 accgccagag tgggcgcact gatccagccc aaccggatgc tcacattggg ctttaccgtc
      661 gattcgccct cctggttttc gattgacgaa gacaaaatct tccgccagac cgacaccacc
      721 gggcagctgg tcgaatcacc gcagccgctg tttcagtatg acttaatcca cccgttcgct
      781 ttcggcgccg gcgccaaagt caatatagcc ggcctcactc tggcgggcga tctgggtttc
      841 accgattggt cgcagatgga gtacaggaat acgcccgatg cccgcctgca gagtgattcg
      901 ctccagcaaa attaccgcga cgtgttgcgg attaacctcg gcgcggagta caccattccg
      961 gtaaccggcg tctccctgcg ggccgggttc ctgtccgatc cgcttccgtt caaatcgagc
     1021 gtgatccaga ccaaccggaa gttctttacc ttcggcgccg gattcctcgt cgatcaggtg
     1081 ctgacgctcg atgcggcagt ggttctgggc gactttggct tcaacaacta cgggaatttt
     1141 ttctacactg agaaatacaa tcttacccgt gtatttgtga ccgcggcttg gcggctgtaa
     1201 gtacggattt gggaattggg atttcggatt tggggaaaac cgccgagccg caccacaacc
     1261 acccgcgagg ccgaacttta gttcggcatg actttaaggc aaaatcctat ggctgtgggg
     1321 ttcgcttttg ggcgaagagc gtcgtcagaa ttacccaaag tacgcgtagg ccggtcggaa
     1381 cgactcgtac cgagacttta gtccggcata atattccctt tcgattcaac cgcttgtggc
     1441 cggacaaaag gcgttgtcac gataacccag agcgttttga gcaacagaag taaatcctta
     1501 ccgatagctg ggcgattcaa gtacgcaaaa tcgtaggcca gtttctccgg caactgtttc
     1561 tggaggtagt agtcttccag gtcaactccc tccgggtggt cgtgcgcttg aaagcggtag
     1621 taatagaggg tgccgggtga agtcaatccg ggtttgacct gaaatatttt cttctgttcg
     1681 gctgaataac ggctcgccag gcggggcact tccggccggg ggccgaccaa actcatttcc
     1741 cctttcagca cgttcaccag ttgcggcagt tcgtcaattt tggttttccg taagatgagg
     1801 ccgatgcggg taatccgcgg gtcacgcggg gcggtcaccc ggggcccgga ctgttcggcg
     1861 cctttgatca tcgaacggta cttgagcatt tcaaaaagga ccccgtcttt gcccacccgg
     1921 gtctgtttga aaaaaacggg tctcccggaa tcgaagagaa tggccagcgc ggcgagcaca
     1981 aagaaagggg aaaggagaac caaagcgaca gttgcgaaaa agagatcgca ggctcgtttg
     2041 ctggctttat acatcagtct ggataacaaa agagccccgc gggcggaagt caacgacggc
     2101 tttgtccctc caggtcgaac ggcagccccg cgaaaaccgg aaaggcacaa tcaatgtctg
     2161 cgggaaggtg gggggctaaa taatctgccg gataagtcga tacaccctac aggcacgcga
     2221 acattgcgcc gaaggagaag ctatgggcga cctgaccata attgccattt caatgtcggt
     2281 tctggctctg acctgggcag tggtccgtat gaacgagcgg gtggaagatc tgcagaagaa
     2341 actgcgccgg ataagcgatc agttcaaccg tgatacggtg ggccaaagga cgcccgcggc
     2401 gccccggccg atgcaagcgg gttccaccta ccagggacca atgcagcccg gacagatgca
     2461 gccggtgggc tgagcccccc tactacggga tttcggcagc cggtttcccg caccctgcga
     2521 gcgaaaactc tttaccacaa aatgaagatc actctgcctt tgacagggtg attttttttt
     2581 gaccactggg aaacgggcaa tgcgcattgc ctcttaccgg tagtccggct cgtctctgag
     2641 ccggtcggta cgactcgatt ccgagacagt cggggtcagg gacgaccccg actaccagga
     2701 gtcaaactcc gtttttccct cgttgaaa
//
LOCUS       DBNGZA010000380         3758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1134769, whole genome shotgun sequence.
ACCESSION   DBNGZA010000380 DBNGZA010000000
VERSION     DBNGZA010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1134769"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2360
                     /locus_tag="AC16FU_08300"
     CDS             102..2360
                     /locus_tag="AC16FU_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2994406.1"
                     /translation="MDTQPAVRQGRRRRVAAGRILLFSFTLLIGWAVCSGTVQAQQST
                     VDTLRILDAFGLPGDTAVIVQIYVRNDSFPVAGVTFRLIFDTTFLTPVFLPPTQTVPF
                     PVRFETVNRGTALPEGGVALDTSATGVVNGLLFAGFSGIPKIERGAGTIMHVFFNIKP
                     GVSLGATSPLRLINDTAQGGLLNALSDTLGNLILPVLANGTLHIQQPPRINRAPVIQP
                     IAAQQVTQGQRLTFTVTAIDADGDPLTLQATALPANATFATVTGDSIASGTFTFVPSF
                     SQEGDVFPIFRATDSVGAITTLSVKITVKKLLRDVLSTISDTSAGKQPVGALAGRGGV
                     LFPIDLANTKDVLGVQFDFNFNPTILGVDSVVPTGRTQGFDRLLFDTIAPGVVRVVAY
                     GLNLQRLQPGVTPTILNVALSVNPAAVTGKYPVTFTNAFISFDPNLPSESLAVENGIF
                     SVDLLGDVNLDARVDVGDAVNCVAFILGSLDTTFRRFDAADVNRDSTLNVLDLAGIEN
                     IIFGRPPVAASPYIGPFARVTVDANVLRASTEGVKINAELPAEVAGAQLVLRCNPTQV
                     VFQAPERTERSGDMLVQYRNFPNGEMRILLYNLNPHKTIAPGSGEILRLPLLALQPTN
                     DSVLPVRLTSVVLSTPGAREIPVEGFSAGAVPREFQLLQNYPNPFNPQTTIKYRIAGE
                     RNANVVVRLQVFNILGEEVVTLVDGPKGAGEHTIIWDGRDKSGQKVSSGVYLYRLVAG
                     SFRETKKMVLVK"
     gene            2386..>3758
                     /locus_tag="AC16FU_08305"
     CDS             2386..>3758
                     /locus_tag="AC16FU_08305"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HIR2994407.1"
                     /translation="MSQKLSWQSVPLSVPAVFSLVVGIGLWGAGDAEAAVIKSVQRGT
                     ATFASGQNTLPVTLTSVDPTKTIVWGGTNWGGGRDASPNANATRVGFDLASATTLNLQ
                     RLGSPTALTVVEWQAIEFSSGVSVQRGVASIGSATATVNVPITTVNLSESFILASVAT
                     GAGAQNADEEWTVRARLTTTTNLELTRNQTGTAITVYWQVVSITGASVQRGLTTVGAG
                     ASSATAAITSVNTASSFLVFNQRAAAASNGVESEYQVRGRVTSATQLTFDRISTSNSV
                     DIAWEVVTLNDGSSVQSSSIAVGSAATSGTATLSSISLSRSASFISVRGGAAGGFQNL
                     DETAFTHNLTNSTTLTVARGGTGTAADVAWFVIQFVGGQPPVLAPIGPKSTNENQLLQ
                     FRVSATDPDLTTPSLSAVNLPAGATFVDSLNGAGSFTWTPSFTQAGTYNVTFIASDGA
                     LADSELVT"
BASE COUNT          751 a         1047 c         1123 g          837 t
ORIGIN      
        1 gcaccagcga cagcggacag ccggtggcta gcggcgtcta cttttcccgc ctaatcaccg
       61 aacagagtga ggcaatcggc aagatgacgc ttctgcgata gatggatacg caaccggcag
      121 tacggcaggg ccgacgcagg cgcgtcgccg ctggcagaat tcttctcttc agttttacac
      181 ttttgattgg ctgggcggtt tgctcgggta ccgttcaggc gcagcaatcg accgtcgata
      241 cgctgcgaat tctggacgcg ttcggcctcc cgggcgacac cgcggttatc gtacaaatct
      301 atgtacggaa tgattcgttc ccggtcgcgg gagtaacctt ccgcctcatt tttgacacaa
      361 cgtttctaac accggtcttc cttcctccaa cccagacggt gccatttcct gtccgttttg
      421 aaacagtaaa ccggggtacc gcgttacccg aagggggtgt cgccctggac accagcgcaa
      481 ccggcgtagt gaacggcctt ctgttcgccg gtttcagcgg tattccgaag attgagaggg
      541 gggcgggaac tatcatgcac gtttttttca acatcaaacc tggagtgagt ctgggtgcaa
      601 ccagtccact acgtttgatt aatgacaccg cccagggagg gctgttaaac gcgctgtcgg
      661 ataccctcgg taatctcatt cttcctgttc tggctaacgg cacgctgcac atccagcagc
      721 cgccgcgcat caaccgggca ccggtcattc agcccattgc ggcgcagcag gtgacgcagg
      781 gacaacggct gacctttacc gttaccgcca tcgacgccga cggcgaccca ctgaccctgc
      841 aggccaccgc gctcccggcg aatgccactt tcgcgacggt aacaggcgac agcatagcga
      901 gcggcacgtt cacttttgtt ccgagtttca gccaggaagg ggatgttttc ccgattttcc
      961 gcgccacgga ctcggttggc gcaatcacaa ccctctccgt aaaaatcacc gtgaagaaat
     1021 tgctccggga tgttctctcg acgatttcag atacgtcggc gggcaaacag ccggtggggg
     1081 cgctggcggg ccggggtggt gtgctctttc ccattgacct ggctaatacc aaagatgtgt
     1141 tgggggtgca gtttgacttc aactttaatc cgacaatcct cggagttgat tcagtggtgc
     1201 caaccggacg cacgcaagga ttcgatcgct tgcttttcga tactatagct ccgggagtgg
     1261 ttcgagttgt ggcttacggc ctcaaccttc agcgactcca gccgggagtg acgcccacga
     1321 ttctcaatgt tgcgttgagt gttaatccgg cggcagtaac agggaagtac cccgtgacct
     1381 tcactaacgc tttcatttcg ttcgacccca atctcccttc agagtcgtta gcggtggaaa
     1441 acggcatttt ttccgtggat ctcttgggtg acgttaatct agacgcccga gtggatgttg
     1501 gcgacgcagt gaattgcgtc gcctttattc ttggcagctt agacacaacg tttcggaggt
     1561 ttgatgctgc cgatgtgaac cgagacagca cattgaatgt acttgatctg gccggtattg
     1621 agaacatcat cttcggcaga ccgccggtag cggcgtcacc ctacatcggg ccctttgccc
     1681 gcgtgacggt cgatgccaat gtcctgcgtg caagcacgga aggggttaag atcaatgcgg
     1741 agctgccggc ggaggtggcc ggggcgcagc tcgtcctgcg atgcaatccc acccaggtag
     1801 tctttcaggc cccggagcgc acagagcgca gcggggatat gctggtgcag taccgcaatt
     1861 tccccaacgg cgaaatgcgg attttgctgt acaatttgaa tccgcacaag accattgcgc
     1921 cagggtcggg ggaaattctg cgtttgcccc ttttggctct ccaaccgacc aacgattctg
     1981 tgctgccagt tcgattaacg tcggtagttc tgtccactcc gggcgcgcgg gagattccgg
     2041 tggagggatt ttcagcggga gcggtgcccc gagagttcca gctacttcag aattacccca
     2101 atccgttcaa tccgcaaacc acgatcaagt accgcatcgc cggcgagcgc aacgccaacg
     2161 tggtggtccg gctgcaggtg ttcaacatcc tgggcgagga agtggtgact ttggtggacg
     2221 ggccgaaagg ggcgggtgag catacgatca tctgggatgg gagagacaaa agcggacaaa
     2281 aagtcagttc cggggtgtac ctttaccgac tggtagcggg ctcattccgg gaaacgaaaa
     2341 aaatggtttt ggtgaaatag gatttctctt atgcgcaggc acagcatgag ccaaaagctc
     2401 tcctggcaga gcgtacccct atccgttccg gcggttttct cgcttgtcgt cggcatcggt
     2461 ctttggggtg ctggcgatgc agaagcggcg gtcatcaagt cggtgcagcg ggggacggcc
     2521 acttttgcgt ccggacagaa tacactgccg gtgacgttga cctccgtcga cccgaccaag
     2581 accatcgtct ggggtggaac caactggggt ggtgggcgag acgcctcgcc aaacgccaac
     2641 gcgacacggg tggggttcga tttggcttcc gccacgactt tgaatttgca gcggttgggg
     2701 tcgccgaccg ctttgacggt ggtggaatgg caggcgatag agttcagttc cggcgtttcg
     2761 gttcaacgcg gggtggcctc tattggcagt gcgactgcaa cagtcaatgt tccgattacc
     2821 acagttaatc tgtctgagag ttttattttg gcttccgtag cgacgggtgc cggggcccaa
     2881 aacgccgacg aggagtggac tgtacgagcg cggcttacaa cgacgaccaa cctggagttg
     2941 acccgcaatc aaaccgggac ggctattacg gtttattggc aggtagtttc cattacaggc
     3001 gcttcagttc agcggggact gacgaccgta ggcgcgggtg cttcttcggc tacggcggcg
     3061 atcacttcgg tcaacaccgc gtccagtttt ttggttttca accagcgggc ggcggcagcc
     3121 agcaacggcg tcgaatcaga atatcaggtt cgaggccggg taaccagtgc tacccagctc
     3181 acctttgacc gcatttcgac ttcgaacagc gtggatattg cctgggaagt ggtaaccctg
     3241 aatgacggtt ccagcgtaca gtccagcagc attgcggtgg gttcggcggc aacttcaggc
     3301 acggcaactc tcagtagcat ctctttatcg cgcagcgcct catttatcag cgtgcgaggt
     3361 ggagcggcgg ggggatttca gaatttggat gagaccgctt tcactcacaa tttaaccaat
     3421 tccactacct tgacggtggc acgcggggga accggcacgg cggcggatgt ggcctggttt
     3481 gtgattcaat tcgtcggcgg ccagccgccg gttctggcgc cgataggccc gaagtccacc
     3541 aacgagaatc agctgttgca gttccgggta tcggcgactg atcccgactt aacgacgccc
     3601 tcgctttcgg cggtgaactt gccggctggg gcgacgttcg tcgattcact gaatggcgcc
     3661 ggctccttca cctggacgcc gtcgttcacg caggcgggga cctacaacgt cactttcatt
     3721 gccagcgatg gcgcgctggc ggatagcgaa ctcgtcac
//
LOCUS       DBNGZA010000381         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2832378, whole genome shotgun sequence.
ACCESSION   DBNGZA010000381 DBNGZA010000000
VERSION     DBNGZA010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2832378"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16FU_08310"
     CDS             complement(<1..301)
                     /locus_tag="AC16FU_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase"
                     /protein_id="HIR2994408.1"
                     /translation="MGHELPGIGKISPEIFDELIYPHLGKRLKTVLVGPRHGVDIGVV
                     DLSHGQVMATTTDPVFIVPEYGFHRAAWFAIHILCSDAVTSALPLKYLSIDLNLPL"
     gene            complement(453..1238)
                     /locus_tag="AC16FU_08315"
     CDS             complement(453..1238)
                     /locus_tag="AC16FU_08315"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR2994409.1"
                     /translation="MPPGLGLAASVILGLLVGSFLNVVIYRLPRELSVIKPRSHCPKC
                     CTPIKWYHNVPLFSFLFLGGKCAYCGVKISRRYPLVEAVTAAFFAVACLTIGVRPQLP
                     LVWFLFAAFVAIFFIDLDFQLIPDKITLPGILLGLVFAALTPEIGWLQGLIGLLVGGG
                     AFWFVAVAGEFMFKKESLGGGDIKFAAMVGAFMGWQKLIVIFLGASVLALLWFGISSL
                     LVRGKTQNHQIPFGPYLTVASVVAFFFGDRLIHFYLAYFLSLR"
     gene            complement(1238..>1544)
                     /locus_tag="AC16FU_08320"
     CDS             complement(1238..>1544)
                     /locus_tag="AC16FU_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994410.1"
                     /translation="KVAHSGCRWVISLPNEFHLWRRLAIFFGRNDLVGHAAPHLRLFD
                     FPAMERFFAAAGLAVTKSCVTAVAPPGGQFIKPPAQGLAETFPGLFALSGIFLLEER"
BASE COUNT          388 a          445 c          465 g          246 t
ORIGIN      
        1 tgagcgggag attgaggtca atggagagat acttgagcgg cagggcgctc gtgaccgcat
       61 ccgagcaaag gatgtggatg gcgaaccagg ccgcccggtg gaacccgtac tccggcacga
      121 tgaagacggg gtcggtggtc gtcgccatca cctgcccgtg gctcagatca acaacgccga
      181 tgtcgacgcc gtgccggggg ccgactaaaa cggttttgag ccgtttcccc aaatgagggt
      241 aaatcagttc gtcgaatatt tccggggaaa tcttgccgat tcccggtagt tcgtgtccca
      301 tcccgcctcc tgaacaaaaa ccagccctga gccgcaaaac ttattgtgtg accaccggac
      361 agtcaactcg gatgcgcggc gggggcgctc ggtacgagtc gtattgacca cgcctacgtg
      421 gagaagagtt ttctcaaatg gctttgtctt gattaccgga gcgaaagaaa gtaggcgaga
      481 taaaagtgaa ttagccggtc accgaagaag aatgcgacga cggaggccac tgtcaaatac
      541 ggcccgaagg gaatctggtg gttctgcgtt ttgccgcgta ccaggagact ggatatcccg
      601 aaccataaga gcgccagaac cgaggcaccc aaaaagatga cgatcaactt ctgccagccc
      661 ataaacgccc ccaccatcgc ggcaaacttg atgtctcccc cgcccaaact ttcctttttg
      721 aacatgaact cacccgcaac cgccacgaac cagaaggcgc cgccgcctac gagcaggccg
      781 atcaggcctt gcagccagcc gatctctggc gtcaaggccg cgaagaccag accgagcaga
      841 atgcccggga gggtgatttt atcggggatt aactggaaat ccaagtcgat gaaaaaaatc
      901 gccacaaatg cggcaaagag aaaccagacc agaggcagct gcggccgcac accaattgtg
      961 aggcaggcga ccgcgaagaa ggcggcggtc accgcctcca ccaaggggta gcgccgggaa
     1021 attttgaccc cgcagtaggc gcacttgccg ccgaggaaaa gaaaactgaa aagcgggacg
     1081 ttgtggtacc atttgatcgg cgtgcagcat ttcgggcaat gtgagcgggg tttgatgacc
     1141 gacagttccc gcgggagccg gtagatcacc acgttgagga aactgccgac cagaagaccc
     1201 aatatgacgc tcgccgccag acccaatccc ggaggcatca gcgttcctcc agaaggaaaa
     1261 tgccggaaag cgcaaagagc cccgggaagg tttcggccag cccttgcgcc ggcggcttaa
     1321 tgaattgccc gccgggcggc gccacggcgg tgacgcagga cttggtgacg gccagaccgg
     1381 ccgccgcaaa gaaccgctcc atcgccggga aatcgaagag ccgcaggtgg ggcgcggcat
     1441 gccccaccag atcgttccgg ccaaagaaga ttgccaaccg ccgccagagg tgaaactcat
     1501 tgggcagaga gatgacccag cggcagccgg aatgggccac tttt
//
LOCUS       DBNGZA010000382         1074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1455490, whole genome shotgun sequence.
ACCESSION   DBNGZA010000382 DBNGZA010000000
VERSION     DBNGZA010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1455490"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="AC16FU_08325"
     CDS             complement(<1..802)
                     /locus_tag="AC16FU_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2994411.1"
                     /translation="MPKPKRLITAEDLLKFKWIRSVALSPDEKQIAYTLEWIDETKKK
                     YWSNLRVVPATGGMPRPFTQGKVKDREPVWSPDGKLIAFFSVREEKDGIYVIPADGGE
                     ARQVVTLDGSFVSLSWSPDGKKLLCAFRRNDPPQEDGKSVRPVGKDESASGGEKKKEA
                     PVFRHITRLFYRLDGEGFLPKDRYHIWTFDAASGKGKQLTFGRFDELGPAFSPDGRWI
                     AFASNHQPDPDRAFLRVDLWLVPATGGKLKKIPTPAGPVESPAWSPDGK"
BASE COUNT          212 a          318 c          309 g          235 t
ORIGIN      
        1 tcttcccgtc cggtgaccag gcggggctct cgaccggccc ggcgggcgtg ggaattttct
       61 tcagttttcc tccggttgcg ggaacgagcc acaggtcaac ccgcaggaat gcgcggtccg
      121 ggtcgggttg gtgattggag gcaaaagcaa tccagcggcc gtcgggcgag aaggccgggc
      181 cgagctcgtc aaaacggccg aaggtcaact gttttccctt cccggaagcg gcgtcaaatg
      241 tccagatgtg gtagcggtct ttgggcaaaa aaccttcgcc gtcgagccgg tagaagagcc
      301 gggtgatgtg ccggaaaacc ggcgcttcct ttttcttttc cccgcccgaa gcggactcgt
      361 ctttccctac gggtcgtacc gactttccgt cttcttgagg cggatcattt ctgcggaagg
      421 cgcagagcag cttctttcca tccggcgacc aggagagcga gacaaaagaa ccgtccagcg
      481 tgaccacttg acgtgcttcg ccgccatcag ccgggatgac gtaaatgccg tccttttcct
      541 cgcgcacgga aaagaaggcg atgagcttcc cgtcgggcga ccagaccggc tcccggtctt
      601 tgacttttcc ctgcgtaaag ggccgcggca ttccgccggt tgcgggaacc acccggagat
      661 tcgaccagta tttctttttc gtctcgtcga tccactctaa ggtgtaggcg atctgctttt
      721 catcgggcga gagcgccacc gagcggatcc acttgaattt gagcaaatct tcggcggtga
      781 tcagacgttt cggctttggc atacactcct caattcgggt ttcattcacg gacgaagggc
      841 tcaatttgca tcaaaatgga atttgacaca agccgaactt gcgtttcagc catcggggcg
      901 ataatttgcg tggaactttt cattaccggc aggggcaaca atgccgccag cgaaaaccac
      961 gcctgttttg cagccgcgag ctacacttgt cgaccgggac ttttccgact gcgccctggc
     1021 ggtccccggc tccgccatcc acctcatcac ccaaaagatc gaggaactgg aacg
//
LOCUS       DBNGZA010000383         2308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2409052, whole genome shotgun sequence.
ACCESSION   DBNGZA010000383 DBNGZA010000000
VERSION     DBNGZA010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2409052"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="AC16FU_08330"
     CDS             complement(<1..1066)
                     /locus_tag="AC16FU_08330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR2994412.1"
                     /translation="MAKTKAQPGSDAMEKLVSLAKRRGFIFQSSEIYGGLTSCWDYGP
                     LGVELKNNLKAFWWKAMTQLRDDVEGIDAAILMHPKVWETSGHVASFTDPLRDCKNCK
                     MRFRADEVTNSTCPNCGGELTAARQFNLMFKTHMGPVEDDASVVYLRPETAQGIYVNF
                     LNVIGPARQKVPFGIAQIGKAFRNEISPGNFIFRSREFEQMEMQFFIHPSEDEKWLDY
                     WKEQRLQWYFDLGIRKEKLRFHQHGPKELAHYAKAAYDVQYEFPFGWQEMEGIHNRTD
                     FDLSRHKEATGKDLSYFDEKTNTRFVPYIIETSAGVDRTLLVTLVDGYYEEEVKGEKR
                     AVLRLSPKIAPIKAAVFPLVN"
     gene            complement(1069..1830)
                     /gene="recO"
                     /locus_tag="AC16FU_08335"
     CDS             complement(1069..1830)
                     /gene="recO"
                     /locus_tag="AC16FU_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR2994413.1"
                     /translation="MAISKGEGIILRERLFGETSKVITLVSPQQGKFALVAKGARGPK
                     SRYGSILDRFAHISFVFYERRGEGMAYLSQADLIRAYFGVAADLSRFACASALVELTD
                     RLVTGEEPVPAIFALLRDALGLCETVGLDDLEKLFWFFCYRMLRELGYRPHLDACAVC
                     QKTVEGENLFFSPEKGGLVCRRCANPRGTYQRIAAGTVTMLKQAEQSSLEEVVRAKIS
                     AQQVGETANLLRGMLTYQVGVTEPIRSLEFMVKAS"
     gene            complement(1817..2179)
                     /locus_tag="AC16FU_08340"
     CDS             complement(1817..2179)
                     /locus_tag="AC16FU_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR2994414.1"
                     /translation="MSAQPPIIIRLTATARSRGWQKYLVRFGVLAAFVFLGYSLVGGN
                     FGLLRIRKLTRERQALETQKLHATVELYDLVRLSEQLRTDRRFGEYFARQKYHFARPE
                     EKVYIFRAPESVTGNGDQ"
     gene            complement(2180..>2308)
                     /locus_tag="AC16FU_08345"
     CDS             complement(2180..>2308)
                     /locus_tag="AC16FU_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994415.1"
                     /translation="SASLSRYERIAKYNLLLRLEEELGDRARYFGSQLKVREAVKA"
BASE COUNT          449 a          698 c          680 g          481 t
ORIGIN      
        1 ggttcaccaa gggaaagacc gcggccttga tgggggcaat tttgggagat agacgcagga
       61 ccgcccgctt ctcccctttc acctcttcct cgtaatagcc gtcaaccaaa gtgaccagaa
      121 gcgtccggtc caccccggct gaggtttcga tgatgtaggg cacaaagcgg gtgttggtct
      181 tctcgtcaaa gtaggagagg tctttgccgg tggcttcctt gtggcgggac aaatcgaaat
      241 cggtgcggtt gtgaattccc tccatctcct gccacccgaa ggggaactcg tactgaacgt
      301 cataggccgc tttggcgtag tgggctaatt ccttcggccc gtgctggtgg aagcgcagtt
      361 tttctttgcg gatgcccaaa tcaaaatacc actgcagccg ctgctctttc cagtagtcca
      421 gccacttttc gtcttccgac gggtggatga aaaactgcat ctccatctgc tcgaactccc
      481 gcgagcggaa gatgaaattc cccggcgaaa tttcgttgcg gaaggccttg ccgatctggg
      541 cgatcccgaa gggcactttt tgccgggccg gcccgatgac gtttaagaag ttgacgtaaa
      601 tcccctgcgc cgtttcgggt cggagataga ccaccgaggc gtcatcttcc accggcccca
      661 tgtgggtctt gaacatcaaa ttgaactggc gcgccgctgt cagctctccc ccgcagttgg
      721 ggcaggtgct gtttgtgact tcatccgcgc ggaagcgcat cttgcagttc ttgcagtcgc
      781 gcaaggggtc ggtgaaactg gccacgtgcc cggaggtttc ccagactttc gggtgcatca
      841 ggatggcggc gtcgattccc tcgacgtcgt cgcgcaactg cgtcatcgcc ttccaccaga
      901 aggccttgag gttgtttttg agttcgacgc ccaacggccc gtagtcccag cagctggtga
      961 gtccgccgta aatttccgag gactggaaga tgaacccgcg gcgcttggcc agcgaaacca
     1021 acttttccat tgcgtcggag ccgggctgtg ctttagtctt ggccatgttc aggaagcctt
     1081 caccatgaat tccaaggagc ggatcggttc cgtcacgccg acctgatagg tcagcattcc
     1141 ccgcaggaga ttggcggttt ctccaacctg ttgcgcggag attttcgcgc gaacgacttc
     1201 ttcaagggaa ctttgctcgg cctgcttcaa catcgtgaca gtccccgccg caatccgctg
     1261 gtaggtcccc cgcgggttgg cgcagcgccg gcagacgagc ccgccttttt ccggactgaa
     1321 aaagagattc tcgccttcca ccgttttctg gcagacggcg caggcatcga ggtggggccg
     1381 gtagcccagt tcccgcagca tccggtagca gaaaaaccag aagagtttct cgaggtcgtc
     1441 cagcccaaca gtttcgcaca accctaaggc gtcgcgcagc aaggcaaaaa tcgcgggcac
     1501 cggctcttcc cctgtcacca gccggtcggt caattccacc aatgccgagg cgcaggcaaa
     1561 gcgggagaga tcggcagcga cgccgaagta ggcgcggatc aagtcggcct gcgaaagata
     1621 ggccatcccc tcgccccgcc tttcgtagaa gacgaaactg atgtgggcaa agcggtcgag
     1681 aatgctgccg tagcggctct tgggcccgcg cgcgcctttg gccacgagag cgaatttgcc
     1741 ctgctgggga gaaacgagcg tgattacttt ggaggtttcg ccgaagagcc gctcgcgcag
     1801 gatgatcccc tcacctttac tgatcgccat ttccggtgac tgattccggg gcccggaaga
     1861 tgtagacctt ttcctcgggc cgggcgaagt ggtacttctg gcgggcgaag tattccccga
     1921 aacgccggtc ggtacgcaac tgttccgaca ggcgcaccag gtcgtacagt tcgacggtgg
     1981 cgtgcagctt ttgggtttcc agcgcctgcc gctcgcgggt caacttgcga atccgcaaga
     2041 gaccgaagtt cccgcccacg agcgaatagc ccagaaaaac gaacgccgcc agaacgccga
     2101 accgcaccag atatttctgc cagccccggc tgcgggccgt tgcggtcagg cggatgataa
     2161 tcggcggctg ggcgctcatt tacgctttca ccgcctcccg cactttcagt tgcgagccga
     2221 agtaccgcgc ccggtcaccc aactcttcct caagccgcag caacaggttg tacttggcta
     2281 tccgctcgta gcgggagagc gacgcgga
//
LOCUS       DBNGZA010000384         2630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1135974, whole genome shotgun sequence.
ACCESSION   DBNGZA010000384 DBNGZA010000000
VERSION     DBNGZA010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1135974"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2563)
                     /locus_tag="AC16FU_08350"
     CDS             complement(<1..2563)
                     /locus_tag="AC16FU_08350"
                     /inference="COORDINATES: protein motif:HMM:NF026255.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2994416.1"
                     /translation="MFSWIRLRPDSLIFFLLFATSFLLLFSNAEAQQPKAAKRGTLAG
                     TVKSAATGEALPGASVFIKELALGQAADQEGNFRFTNLRPALYTVEVSLIGYVVQRIN
                     AVAVRAEEETLQEVLLQTSPLPLGEEIQVIGKRPLLDVNQPQVTRSLDATDLARSAVT
                     ELKEAIASQPGAVRFENEIHLRGGRSYENEVIVDGVPASDPLLRSGFGLEINPAVIEQ
                     LNLRTGGLPAEYGQATGGVVEILTRDPAPVFSGRVAYRTDKFGFGAPFDFNSDYAVLE
                     LSGPEPLSQRLLKNIGLRLPGSITFLVSTNFAFTDGYLGHARSVFTSTLFGGRHLPRE
                     DSRFNGLAKVVWKFDPNHKLSYLVQQSITVNQDRSVLETRIRQVTCSYGYPFVYENLL
                     DNYNVFTHWANLEALTWEHKLSPQWSYDLKLSRFFSNLHSDVNGKEWFQYVKPTDNEP
                     YTVTPLPDSTFFVTRGDGFFDQGDGDTWYDHTWENFLLQGSNHLAVKPNWQTQFGFSA
                     AHRRLEVADIFQPYAGAGGLGADLYQTGTGDAALYFDNEINLKGAIVRAGLRYDLWWP
                     GGYVERVLADTTVPFLPDSLRQAFRRQTFSFFGHRAKDVLSPRFGMSNLITGNLSFIF
                     SYNRLAKWPNPQFVYANLEPRRNSGAYQLFGNPNLNPEKVTLIEAGVKYLIGANDALS
                     IAGYNKSIDDYISAIGVTPDTTNPTQSYLVYFNFDFATSQGVEVEYRKREGDWFEGKV
                     SAAVSRAKGEHALPSDLLRGVNSRAQGRLLEEINFDWDKPWQVNLSANFRALRNRRYR
                     VFGLTLPDNWNGNLFFWAQAGKRYTKFQKAVNPGTGEVSYVQVGKVNDRLGPYWGS"
BASE COUNT          552 a          765 c          771 g          542 t
ORIGIN      
        1 acgagcccca gtacggcccc agccggtcat tcaccttgcc tacctgtacg taactcactt
       61 ccccggtgcc ggggttgacc gctttttgaa acttcgtgta tcgtttcccc gcctgcgccc
      121 agaagaagag attgccgttc cagttgtccg gcagggtgag gccgaaaact cggtagcgcc
      181 ggttgcggag ggcacggaaa ttggcggaaa gattcacctg ccagggctta tcccagtcga
      241 aattgatttc ctcgagcagc cgcccctgcg cccggctgtt caccccgcgc agtaggtcgg
      301 acggcaaagc gtgctcccct ttggcgcgcg aaacggcggc gctcaccttt ccctcaaacc
      361 agtcgccttc ccgtttgcgg tattccactt ccactccctg ggaggtggca aaatcgaaat
      421 tgaagtagac aaggtatgac tgggtcggat tcgtggtgtc cggcgtaacc ccgatggcgg
      481 aaatgtagtc gtcaatcgac ttgttgtagc cagcaatgga aagcgcgtcg ttggcgccta
      541 tgaggtactt cacccccgct tcgatcaggg tcactttttc gggattgagg ttggggttgc
      601 cgaagagttg gtaggccccg gaattgcggc gcggttccaa attcgcgtag acgaactggg
      661 gattgggcca cttcgccagc cggttgtagg agaagatgaa ggacaggttc cccgtgatca
      721 ggttggacat gccgaagcgc ggggagagca catctttggc gcggtgcccg aagaaagaaa
      781 aagtctggcg gcggaaagct tgccggagac tgtcgggcag gaaaggtacg gtcgtatcgg
      841 cgagcacccg ctcgacatag ccgcccggcc accagaggtc gtagcgcagt cccgcccgca
      901 cgatggcccc tttcaaattg atttcattgt caaaatagag cgccgcgtcg cccgtgccgg
      961 tctggtagag gtccgccccc agcccgccgg cgcctgcgta cggctggaaa atatcggcca
     1021 cctccagccg ccggtgcgcc gcgctgaaac caaactgcgt ttgccagttc ggcttcacgg
     1081 ccaggtgatt gctcccctgc aaaaggaaat tttcccacgt gtgatcgtac caggtgtcac
     1141 cgtctccttg atcgaaaaaa ccgtccccgc gcgtcacaaa aaatgtggaa tcgggcaagg
     1201 gtgtaacggt gtacggctca ttgtcggtcg gtttgacgta ctggaaccac tctttcccgt
     1261 tgacgtcgga gtggagattg gaaaaaaatc tggagagttt gaggtcgtag gaccactgcg
     1321 gcgaaagttt gtgctcccag gtcaaggcct ccaggttcgc ccagtgcgtg aagacgttat
     1381 aattgtccag gagattctcg tagacgaagg ggtaaccgta ggagcaggtc acctgccgga
     1441 tgcgcgtctc taaaaccgaa cggtcctgat ttaccgtaat cgattgctgc accaggtacg
     1501 aaagtttgtg attcggatca aatttccaga ccactttggc taatccgttg aagcggctgt
     1561 cctcccgtgg caggtgccgc ccgccgaaga gcgtactcgt gaaaacgctc cgggcgtggc
     1621 ccaggtagcc gtcagtaaat gcgaaattcg tactgaccaa gaaagtgata gagccgggaa
     1681 gccgcaatcc tatgtttttt aggagtctct gcgagagcgg ttccggcccc gaaagttcca
     1741 gaaccgcgta atcggaattg aagtcaaacg gcgcaccaaa cccgaactta tcagtccggt
     1801 acgctacccg acccgagaag acgggtgcgg gatcacgcgt cagaatctcc accacgccgc
     1861 ctgtggcctg gccgtactct gccggcaggc cccctgtgcg caggttcaac tgctcaatca
     1921 ccgccggatt gatttccagc ccgaatccgg agcgcaggag cgggtccgag gccggcacgc
     1981 cgtccacgat cacttcgttt tcgtaactgc gccctccccg caggtggatt tcgttctcaa
     2041 agcgcaccgc tcccggctgg gacgcgatcg cctctttcag ttcggtgacc gccgagcgcg
     2101 ccaggtcggt ggcgtccagc gagcgcgtca cctgcggctg gttgacgtcc aaaagcggcc
     2161 gcttgccgat cacctgaatt tcttcaccca gaggcaaggg actggtctgc aacagcactt
     2221 cctgcaacgt ttcctcttct gcccggaccg ccaccgcgtt gatgcgctgg actacgtagc
     2281 cgataagcga gacctccacg gtgtagagtg ccggacgcaa attcgtgaag cggaagttcc
     2341 cctcttggtc ggcggcctga cccaaagcta gttccttaat aaaaacgctg gcaccgggca
     2401 aagcctctcc ggtcgcggcg ctttttacag ttccggccaa cgtacccctt ttggcggctt
     2461 tgggctgttg cgcctctgcg ttggagaaca aaaggaggaa agaggtggcg aataggagaa
     2521 aaaagatgag cgagtccggt ctcaaacgaa tccaggagaa catggccatc atagctcaac
     2581 cggcacttcg ctgtaaaatg actttttcgc cttgacctgt caaggagatt
//
LOCUS       DBNGZA010000385         4295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2410081, whole genome shotgun sequence.
ACCESSION   DBNGZA010000385 DBNGZA010000000
VERSION     DBNGZA010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2410081"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="AC16FU_08355"
     CDS             complement(<1..565)
                     /locus_tag="AC16FU_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994417.1"
                     /translation="MIRTLSLFLPVFLLTNSLTSAADHKGHFAVGIEGGLLGPLSAAR
                     QGSDLSSLVEVSIEKFTGTNTVFGVSLLVSESELFLRPGIIPAIERLVVGWPQPKTSR
                     LTQVGVYLRAFNPTKSFVKLYGIIGPSIDRLKDRPGQTKAEYAWGIRGGFGLRLELFP
                     LVSLSFGPRLNFQSPSTFYYTASGGLVL"
     gene            complement(634..1287)
                     /locus_tag="AC16FU_08360"
     CDS             complement(634..1287)
                     /locus_tag="AC16FU_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR2994418.1"
                     /translation="MYVIIVGGGQIGLNLTKLLLGEGHEVLLVEKDPGRYANLADELG
                     EAVIQGDGGDLASLMDTGANRADVLAAVTGADQDNLIICQMAKSLYLIPRTIARVNNP
                     KNEEIFHTLGVDATVSSTRIIGALIEEKIDAGMAIPLLALKGGSVEIFQTQLEEDSPF
                     VGRKIAKVKLPEECIIMALLRGDQVIIPKGDTVLQEKDVLITLTSKEAKEEFRKLFL"
     gene            complement(1289..1690)
                     /locus_tag="AC16FU_08365"
     CDS             complement(1289..1690)
                     /locus_tag="AC16FU_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR2994419.1"
                     /translation="MRVVILGAGRVGAILAKRLLKDKHEVAVIDWRVESFRNLGKDFP
                     GQTVLGTGIDDEVLKKAGIEKADAFIAVSDSDNTNLMAVQVAKARFNTKTAIAWVVDP
                     QRAQSFQEYGLEIVCPTTLVADVIQKKLKGK"
     gene            complement(1696..2256)
                     /locus_tag="AC16FU_08370"
     CDS             complement(1696..2256)
                     /locus_tag="AC16FU_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR2994420.1"
                     /translation="MLSVEITFLGAFLAACFAVAMGGLLWWMLHPPEAVKLLVARAYR
                     SVDALKRILVPTTGLPYSDRGVELACRLGEMQKADIILTYVIEVPRTLPLNAPLPEAE
                     TKAVEALNRAVEIVKLHDLEAIPSVERAREAAEGIIRVAKDNQVDLIVLGMRTNIHPT
                     QAFFGRTTESLIRRAPCEVVIDKLPG"
     gene            complement(2243..2476)
                     /locus_tag="AC16FU_08375"
     CDS             complement(2243..2476)
                     /locus_tag="AC16FU_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994421.1"
                     /translation="MSRLTEKFSRLSPGDKYRFFTTGSFFVLGAVIAVRGLAVGIFPV
                     LMGIAFVGFGIYRFYYFYRYFRQGRSKAPHAVR"
     gene            complement(2460..4052)
                     /locus_tag="AC16FU_08380"
     CDS             complement(2460..4052)
                     /locus_tag="AC16FU_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR2994422.1"
                     /translation="MRNRSLKPSPPPTTPGAELRRDVGVWGSFMWGYSDVGADIYVAL
                     GLVMAYAQGAASVVFALAGSVYIMIGLAYTELASAYPVAGGGPYFTLRGLGDFWGFVG
                     GSALLLDYTIDIALFATASAGYLNFFLPQLIGRSIQSFAVTLGPFHNVNPVWCLETLL
                     LIAILILLNIRGVRESTFLQSFIGIVDLLAEGSIIIFGFLFAWKPELLALQWKTQFPS
                     FHNLVYGSSLAIISFVGLESISQAAQETKRPATVIPRTSLTLIFTVFIFAVSFSTLGL
                     GVFPWQVFRDHMGDPVAYLAHGIPFIGFIAGPFAAILGGTILFISANSGIFGASRLTY
                     SLAQFGSLSPWFCSVHPKFHTPVRTIVIFSMIGVVQTILAFLTPSAMDTLGNMYAFGA
                     ATGYTLVFIALIKLRFSDPYTPRPYRVPLNFKLRYKGRRVDFPVLGVIGTLGIATILF
                     EVVLTHAIGRIAGPAWVLLCFVYYAWYRKKAGFPVFRSIRHDWEREQIDVLTSAEEFD
                     LLEQYKNALKEKRKEPLHVPAD"
BASE COUNT         1063 a         1203 c         1154 g          875 t
ORIGIN      
        1 acaacaccag accgccgcta gcggtgtagt aaaaagtgct cggggattga aagttgagcc
       61 gcggaccaaa agaaagtgaa accaaaggaa acagctcgag ccgtaatccg aaaccgcctc
      121 ttatacccca agcgtattca gcctttgttt gtcctggtct gtctttgaga cggtctattg
      181 atggaccaat aataccgtag agcttcacaa aggatttggt tggattgaaa gctcgtaaat
      241 aaactcccac ctgggtcagt cgagacgttt taggctgggg ccaaccaacc actaaccgct
      301 cgattgctgg aatgatccct ggccgcaaaa aaagctccga ttcgcttacg agtagactga
      361 ctccaaatac agtatttgtc ccagtaaatt tttctattga aacttctact agagaactca
      421 aatccgatcc ttgtcgtgcg gcggataagg gaccaagcaa tccaccttcg ataccaacgg
      481 caaaatggcc tttgtggtca gcggcagaag taaggctgtt cgttaaaaga aaaacgggta
      541 aaaagagcga aagagtccta atcataatac ccttctctat tcttgctacg ttggggagtt
      601 cacattgtag gaggtacttt tcagaaaaga aaactacaaa aacaacttcc tgaactcctc
      661 cttcgcctct ttgctcgtca aggtaatcaa aacgtctttt tcctgcagta ccgtatcgcc
      721 tttgggaata atcacctggt ccccccgcaa aagcgccata atgatgcact cctccgggag
      781 tttgaccttc gcgattttgc gcccgacgaa gggcgaatcc tcctccaact gcgtctggaa
      841 aatctccacc gacccgccct tgagcgccaa aagcggtatc gccatccccg cgtcaatttt
      901 ttcttcaatc agagcgccga tgatgcgggt ggaagaaacc gtggcatcga cgcctaaggt
      961 gtggaaaatc tcttcgtttt tcggattatt cacccgggca atcgtgcgcg ggataagata
     1021 gagactcttg gccatctggc agatgatgag gttgtcctga tccgcgccgg tgaccgcggc
     1081 cagaacgtcg gcccggttgg ccccggtgtc catcaaggaa gccagatcgc cgccgtcgcc
     1141 ctgaatcacc gcctccccca attcgtccgc taaattggcg tagcgccccg ggtctttttc
     1201 gaccaaaagc acctcgtgcc cttcgcccaa gagcagtttg gtcaaattga gcccgatctg
     1261 cccgccgccc actatgatga cgtacatttc atttcccttt gagtttcttt tggatgacgt
     1321 ccgccactaa ggtcgtggga cagacgatct ccagcccgta ctcctgaaac gactgcgccc
     1381 gctgcggatc gaccacccag gcaatggcgg ttttggtgtt gaagcgtgcc ttggctacct
     1441 gaactgccat gagattggtg ttgtcgctgt cggaaacggc aatgaaggcg tcggccttct
     1501 cgatgccggc ttttttgaga acctcgtcgt cgattccggt gcccagaacc gtctgtccgg
     1561 gaaaatcttt gcctaaattg cggaacgatt ccacccgcca gtcgatgacg gcgacttcgt
     1621 gcttgtcttt gagcaggcgc ttggccagaa tcgcgcccac ccgccccgcc cctaagatga
     1681 ccactcgcat tcgtattacc ccggcagttt gtcgatcacc acttcgcacg gcgcccggcg
     1741 gataagactt tccgtcgtcc gcccgaagaa cgcttgcgtc ggatggatgt tggtgcgcat
     1801 gcccagcaca atcaaatcga cctggttatc tttggcaacg cggatgatcc cttccgccgc
     1861 ctcccgcgcc cgctccaccg acgggattgc ctctaagtcg tgcaacttta cgatttcgac
     1921 ggcccggttc aaggcctcta ccgctttggt ttccgcctcc ggcaaaggcg cgttcaaggg
     1981 caaagtccgc ggcacctcaa tcacgtaagt caagataata tcggctttct gcatttcccc
     2041 caagcggcag gccagttcca ccccccggtc ggaatagggc aagccggtgg tggggactaa
     2101 aatccgcttc aaggcatcca ccgagcggta ggcgcgcgcc accagaagct tgaccgcctc
     2161 cggcgggtgg agcatccacc agagcagccc gcccatggcg actgcaaaac aggcggccag
     2221 aaaagcgccc aaaaatgtta tctcaacgga cagcatgcgg cgcttttgac cggccctgcc
     2281 ggaaatagcg gtagaagtag tagaaccggt agatgccaaa tcctacgaaa gcaattccca
     2341 taaggactgg gaaaatacca accgccagac cccggaccgc aatgaccgcg cccaggacaa
     2401 aaaaggaacc ggtggtgaaa aaccggtact tgtcccccgg cgagagtcgg gaaaattttt
     2461 cagtcagccg ggacatgcaa cggttccttc cttttttctt tgagggcgtt cttgtactgc
     2521 tccagcaggt caaactcttc ggctgaggtg agcacgtcga tttgctccct ctcccagtcg
     2581 tggcggatgc tccgaaaaac ggggaatccc gccttcttcc ggtaccaggc atagtagacg
     2641 aagcacaaga gcacccaggc gggcccggct attctgccta tggcatgtgt caggacgacc
     2701 tcaaaaagaa tggtcgcaat ccccaaagtt ccaatgactc ccaggacggg aaaatctacc
     2761 cttctgccct tgtagcgaag tttaaaattg agaggaactc gatacggtct gggtgtatag
     2821 ggatcgctga aacggagctt gatcaaggcg atgaagacca gcgtataccc ggtggcggca
     2881 ccgaaggcgt acatattgcc aagcgtatcc atggccgaag gggtgaggaa ggccaagatt
     2941 gtttgaacca caccgatcat cgaaaaaatg acgatggttc tgaccggggt gtgaaatttc
     3001 ggatgcacgc tgcaaaacca agggctgagg ctgccgaact gggccagaga ataggtcagc
     3061 cgggaggcgc cgaatatccc gctgttggcg gagatgaata aaatcgtgcc ccccaaaatt
     3121 gcggcgaagg gcccggcgat aaagccgatg aaaggaatcc cgtgcgctaa atatgccacc
     3181 gggtcgccca tgtgatcccg aaagacctgc caagggaaaa cacccagacc cagagtggaa
     3241 aacgataccg caaagatgaa aacggtgaat atcaaggtga gtgaagttcg cggtatgacg
     3301 gtggccggac gtttggtttc ctgggccgcc tgagagatcg attccagccc tacaaaggaa
     3361 atgattgcca aggaggaacc gtagacaagg ttgtgaaagg aggggaattg ggttttccac
     3421 tgcagggcga gcagttccgg tttccaagca aagagaaacc cgaagattat gatgctgccc
     3481 tcagccagaa ggtctacgat gccgataaaa gactgcagga acgttgactc ccggactccc
     3541 cgtatgttta agagaatcaa aatcgcaatt aaaagtaaag tttccaggca ccacacggga
     3601 ttgacgttgt gaaacggccc gagagtgacg gcgaagctct ggatgctgcg gccgattagc
     3661 tgcggcagaa agaagttcag gtagccggct gaagcagtcg caaagagggc gatgtcaatc
     3721 gtgtaatcca agagaagagc ggaaccgccc acaaagcccc agaagtcgcc taaccctctc
     3781 agagtgaagt atggcccccc accggcgacg gggtaggccg acgccagctc ggtgtaagcc
     3841 aggccgatca tgatgtagac cgatccggct aaggcaaaga ccacgctggc ggctccctgg
     3901 gcataggcca tgaccaagcc cagagcgaca taaatatccg ccccgacatc cgaatagccc
     3961 cacatgaacg agccccaaac gccgacgtcg cgccggagtt cggctcccgg tgtggtgggt
     4021 ggcggagacg gtttaaggct tctgtttctc ataggctaac aaaaaagacc agtgcccacc
     4081 tggtctgtcg aagctgttct ccgctcgtta gagtcgacca aacgtacggt tcgagtctac
     4141 gacgcagact cgctccgagc ggttgcgcca tttttgcttt tcccatacgc atcgccgcgc
     4201 gggctacacc ccccttgggg cgtcaatcca catcccgcgc gaagatagaa cggcggggga
     4261 atatagattg gcggggagga atgtcaaata gagtc
//
LOCUS       DBNGZA010000386         1817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2727124, whole genome shotgun sequence.
ACCESSION   DBNGZA010000386 DBNGZA010000000
VERSION     DBNGZA010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2727124"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1202
                     /gene="kbl"
                     /locus_tag="AC16FU_08385"
     CDS             15..1202
                     /gene="kbl"
                     /locus_tag="AC16FU_08385"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HIR2994423.1"
                     /translation="MIGTVRDFLTKELTGIREAGLYKEERVILSPQGAEIRVREGEVI
                     NFCANNYLGLGNRPELIAAAKQALDSHGFGLASVRFICGTQDIHKELEKKISEFLRTE
                     ATILYTSCFDANGGLFETLLSDDCVVISDELNHASIIDGIRLCKAERRRFKHADMGDL
                     EAQLQGNEKFRIRLVATDGVFSMDGDIAKLDQICALAEKSQALVMVDDSHATGVLGQT
                     GRGSIEHCGVVGKVDIITSTLGKALGGASGGFTSGRKEIIEMLRQRSRPYLFSNTLAP
                     PLVAASIQVLELLSQTTDLRERLEDNTRYFRNEMTKRNFDIKPGVHPIVPIMLGEAKL
                     AQDMARDLLKEGIYVIGFFYPVVPKGQARIRVQVSAAHTRAHLDKAVEAFTKVGRRYG
                     VIA"
     gene            1341..>1817
                     /locus_tag="AC16FU_08390"
     CDS             1341..>1817
                     /locus_tag="AC16FU_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR2994424.1"
                     /translation="MPSLSLDWEKIRPDAVSTLQSLVQFNTVNPPGAEEPVIDYLQDL
                     LQKEGIATEILAAEPTRDNLFAHLPGSSKEPPLLLMSHVDVVGVEPEKWSVHPFSGAI
                     DNGYLYGRGAIDDKGMAAAEAMVLLLLKRLNVKLKRDVVYLAAADEESGGKLGIAWL"
BASE COUNT          395 a          497 c          562 g          363 t
ORIGIN      
        1 agcagggata aacgatgatc ggaacagtcc gggattttct cactaaggaa ctcaccggca
       61 ttcgtgaagc ggggctctac aaggaagagc gggtgatcct cagcccgcag ggggccgaga
      121 ttcgcgtacg ggagggcgag gtcatcaatt tctgcgccaa caattacctc ggtctgggca
      181 accgcccgga gttaatcgcg gcggccaagc aggcgctcga ttctcacggg ttcggcctgg
      241 cctcagtgcg cttcatctgc ggcacgcagg acatccacaa agaattggag aaaaaaattt
      301 ctgagtttct ccggaccgaa gccacgattc tttatacctc ctgttttgac gccaacggcg
      361 gtttgttcga aacgcttttg tccgacgatt gtgtcgttat ctccgacgaa ctgaaccacg
      421 cctcgatcat cgacggcatc cggctctgca aggcggagcg gcggcgcttt aagcacgccg
      481 atatggggga cctcgaagcg caactgcagg gtaacgagaa gttccgcatc cggctcgtcg
      541 ccaccgacgg cgtcttttcg atggatggcg acatagcgaa gttagaccag atttgcgcgc
      601 tggctgagaa gtctcaggcg ttagtgatgg tggacgattc ccacgccacc ggggtcctcg
      661 gccagaccgg acgcggctcc atcgagcact gcggggtggt gggaaaagtg gacatcatca
      721 cgagcacctt agggaaagcg ctgggcgggg cttccggcgg gttcaccagc ggacggaagg
      781 aaatcatcga gatgctgcgc cagcgctcgc ggccttatct tttctccaac accctggcgc
      841 cgcctttggt ggcggcgtca attcaagtct tagaactttt aagccagacg acggacctgc
      901 gtgaacgtct tgaggacaat acccgttact ttcgcaacga gatgactaaa cgtaactttg
      961 acatcaagcc cggcgtgcat ccgatcgtcc cgattatgct cggcgaggcg aaattagccc
     1021 aggatatggc ccgcgacctg ctcaaagagg gaatttacgt catcggcttc ttctatccgg
     1081 tggttcccaa aggccaagcg cggattcggg tgcaggtttc cgccgcgcat acccgggcgc
     1141 atctggataa ggcggtcgag gcgtttacca aagtggggcg gcggtacggg gtgattgcgt
     1201 aattgggtta acagcggcgc tggtagtgcc gcctacgcga ggtagagaaa ggtcacaacc
     1261 cgcgaaggcg ggtcggcacg actcgtaccg aggctcttag ccccgcataa aaaagagatt
     1321 attataaaga ggtgagcagt atgccttccc tttcgctcga ttgggaaaag atcaggcccg
     1381 atgcggtttc aaccctgcaa agtttagtcc agttcaacac cgtcaatccg ccgggggcgg
     1441 aggagccggt gattgactac ctccaggatt tgctacaaaa agaagggatc gccactgaga
     1501 ttctggcggc ggagccgacg cgggataacc tttttgcgca cctgcccggc tcaagcaaag
     1561 agccgccgtt gcttttgatg tcccacgttg atgtggtcgg cgtggagccg gagaagtggt
     1621 cggtgcatcc cttctccggg gccatagaca acggctacct ctacggccgg ggtgccatcg
     1681 acgacaaagg aatggccgcc gccgaggcga tggtgctctt gcttctgaaa cggctgaacg
     1741 tcaaactcaa gcgcgacgtg gtttacctcg cagcggcgga tgaagaaagc ggcgggaagc
     1801 tgggcatcgc ctggctg
//
LOCUS       DBNGZA010000387         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2515486, whole genome shotgun sequence.
ACCESSION   DBNGZA010000387 DBNGZA010000000
VERSION     DBNGZA010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2515486"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1867)
                     /locus_tag="AC16FU_08395"
     CDS             complement(305..1867)
                     /locus_tag="AC16FU_08395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR2994425.1"
                     /translation="MREPFPRSVLSRLQLVFVSSRVVTIFGVIAWLVYSPHREVNAGL
                     VQQLLIAFGLHLLIFYWLAARNFVSLPALYRVTTFFDLAFITLLTWATGGLTSNFYLF
                     YLLAISFASFYFGMAFGLLAAGLSTVAYLGTNAVTFSDLFLGDLLLRILLFWFFATAV
                     GLIAKYLRGSERRLLRALDTLNERTTELEHSQVQIETIYETSRSLGEMHNMHEVLDEI
                     LNIARRILGYPLCSVLLLNAREDRLLLMAKLESGEKQIYPEPIVVSLDGIAGSVARSG
                     KAERVSDVRIDPRYIPGLPGARSEMVVPMISRGNVIGVLNAESEQMSAFFERDQKIFS
                     ILASSAALALENAVMHNKMEELTIVDELTGTFNFRYFTMKLTDELRRANRYSQPLSLV
                     MIDIDWFKKTNDTYGHEKGNQVLQGLAQVIKNCIRDVDILCRYGGEEFVVILPQTDRR
                     DALVIGERVRSQVATTGFGNGSISPMVHITVSVGVTSYPENGRNQDEIVAVVDQALYR
                     AKGSGRNLVCAI"
     gene            2319..>2695
                     /locus_tag="AC16FU_08400"
     CDS             2319..>2695
                     /locus_tag="AC16FU_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994426.1"
                     /translation="MKYRLFGFLLLLASTGYSREIPWSPFEWISFSIGDKTFDRAAIL
                     IPVKLTGTTRALWMQLDTGSDATMVYEIPWKQLHLTVPQAAGDSSKILLSAAIGNQWF
                     DSLWVRMAYGFGDSLTEKEERPV"
BASE COUNT          623 a          712 c          822 g          538 t
ORIGIN      
        1 atgaccccgc ccaggttttt ttcctggatc caacgcagga gttgctccga gggcttcggg
       61 ccggggtagc cgaagacaaa aagttgccct acgaggtttt cggtgccggg catcgggggg
      121 aatataagag ttaattcaca gcagatgaag cagattgaac acaatttccc aaatcggact
      181 ccttaagttc gcaggactga cccagcgcaa tccgaaaccg cagctggcaa tctggtgaag
      241 aagttagcgc aagcgaacag ggcagaggtc cgatcccacc cagccagcca gggtaggata
      301 attcttaaat ggcacagacc aagttgcggc cggagccctt ggcgcggtag agggcctgat
      361 cgaccaccgc cacgatttcg tcctggttgc ggccgttctc cggataggag gtgacgccca
      421 cggagacggt gatgtggacc atgggggaga tggagccgtt gccgaagccg gtggtggcca
      481 cctggctgcg cacccgttcg ccgatcacca aggcgtcgcg gcggtcggtc tggggcagga
      541 tcaccacgaa ctcctcgccg ccgtatcgac aaaggatatc gacgtcgcgg atgcagtttt
      601 tgatcacctg cgccagcccc tggagcacct ggttgccctt ttcgtggccg taggtgtcgt
      661 tggttttctt gaaccagtcg atgtcgatca tcacgagcga gagcggctgg ctgtagcggt
      721 tggcgcggcg gagttcgtcg gtcaacttca tggtgaagta gcggaagttg aaggtgccgg
      781 tcagttcgtc gacaatggtc agttcttcca ttttattgtg catgacggcg ttctccaggg
      841 ccagggccgc ggaggaagcc agaatcgaaa agattttctg gtcgcgctcg aagaaggcgc
      901 tcatctgctc cgactcggcg ttgaggacgc cgatgacatt cccgcgcgag atcatcggca
      961 ccaccatctc ggaacgggcc cccggcaggc cggggatgta gcgcgggtcg atgcgcacgt
     1021 ccgacacccg ctcggccttt cccgaccggg ccaccgaacc ggcaatgccg tccagggaaa
     1081 cgacgatcgg ctccgggtag atctgctttt cgcccgattc cagtttggcc atcaaaagca
     1141 ggcggtcttc ccgggcgttg agcaggagca ccgagcagag ggggtagccc aggatgcggc
     1201 gggcgatgtt caaaatctcg tccaacactt cgtgcatatt gtgcatttcc cccagggagc
     1261 gggaggtttc atagatcgtt tcgatctgca cctgggagtg ttccagttcg gtcgtccgtt
     1321 cgttcaaggt atccagggcg cgcaggaggc ggcgctccga cccgcgcagg tacttggcga
     1381 taagcccgac tgccgtcgcg aagaaccaga agaggagaat ccggagcagg aggtcgccca
     1441 ggaaaagatc ggagaaggtg acggcgttgg tcccgaggta ggcgaccgtg gagagtccgg
     1501 cggccaagag cccgaaggcc atgccgaagt agaacgaagc aaaagaaatc gccagaaggt
     1561 agaagaggta gaagttgctc gtcaggccgc ccgtggccca ggttaaaagg gtgatgaagg
     1621 cgaggtcgaa gaaagttgta acccggtaga gcgccggcag ggaaacgaag ttgcgcgccg
     1681 ccagccagta gaagatcaac aggtgcaggc cgaaggcgat caaaagttgc tgcaccaggc
     1741 cggcattcac ctcccggtgg ggggaataga ccagccaggc gatgacgcca aagatcgtca
     1801 ccacgcggct ggagacgaag actaactgca ggcgcgagag tacggagcgc ggaaacggtt
     1861 cacgcattgc catcgatcaa catcatcctg aaaaagacac acccatccat ccgctgcgag
     1921 ccttcttcct aaatacaaag ccagacgcgg cgggtgtcaa tccctacggg tcgtaccgac
     1981 cgctgttcag tatatcggca gtttcccata aattctacgg aagcggcggg cataccgaca
     2041 catcccaata acccgttttc catcaacggg ttggagcctt acgattttct ctacttatta
     2101 ggatgaggga acttccgaag ccgcgtggtg ctaaattgaa cagaactaca gtggatattt
     2161 tgcgttgcac aattcatcgg caaggtgatg aattactccg ggcacttgcc ttttagcaag
     2221 agaatctaca ttaataaggg gtcagaggag tacaaagtaa atcgcatttg cgcgtcaatg
     2281 caagattgaa gagggcgagc atataaccgg aggctactat gaaatacaga ttgtttggtt
     2341 tccttttgtt gctcgcctcg accggatatt caagggagat cccctggtca cccttcgagt
     2401 ggatcagttt ttcaattggg gataagacgt ttgaccgagc ggctatcctg atcccggtca
     2461 agcttacagg cacaacgagg gctctttgga tgcagttgga caccgggtct gacgctacga
     2521 tggtttatga gataccgtgg aagcaactac atctcaccgt tccccaggcc gcaggagaca
     2581 gctccaaaat cctgttgagt gccgccattg gcaaccagtg gtttgattct ctctgggttc
     2641 gtatggcgta tggctttgga gactcgttaa cagaaaagga ggaacgtccc gtcat
//
LOCUS       DBNGZA010000388         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1137223, whole genome shotgun sequence.
ACCESSION   DBNGZA010000388 DBNGZA010000000
VERSION     DBNGZA010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1137223"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16FU_08405"
     CDS             complement(<1..458)
                     /locus_tag="AC16FU_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR2994427.1"
                     /translation="MQFSLDRLKSSLAKTKDTFLSKITAAIGLHPRIDAGLLQDLEDT
                     LIRSDVGVAVTLRLVEQLKKRAQQNNLTGADAVISLLKDEMRHCLVNGSGVATAKTAK
                     EKPHVILVVGVNGTGKTTTIGKLAYHFARQGQKVLLAAADTFRAGAIDQLA"
     gene            complement(701..>2280)
                     /locus_tag="AC16FU_08410"
     CDS             complement(701..>2280)
                     /locus_tag="AC16FU_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2994428.1"
                     /translation="SGGGDRETGLVGRAARLAAREEEIAVREKQIRESARRLESLGKQ
                     VTALAAKSETQKREMERNRSLLHEQTLEHERFQIESEAQHQQILLLAQERKEIEAHLA
                     RGETAGKEEKADLLALQEERGGVRLETEQLAKEIERLESAESEQVQKIGDARLELVSL
                     QGQFEHAQENSRRLGELVAEIRKSQAEKTSHRLELAALTSREQTSGTSFEDQQAGRLA
                     KEEVLRSAAAAEEQRLDSLRQKRALVEGKVKGQRMSRDEAVARQHQVELALTELKGQL
                     QQITSRLQAESGISPEALAPVSDEEAAHLEDLPGQLQELRTGLDSLGPVNLLALEEYE
                     SQKKRFEFLAQQMADLQKAREQLQTTIEELNRQALEQFNTTFRQVRDNFQAVFAELFE
                     GGEADVTLTGTDDPLEMDIAVFARPKGKKLVTITQLSGGERALTAIALLFALYLVKPS
                     PFCILDEVDAPLDDANIDRFLKLIKRFARNTQFIVITHNKKTMEAADVLYGVTMEEPG
                     VSRIVSVRLSELVPEMA"
BASE COUNT          387 a          720 c          622 g          551 t
ORIGIN      
        1 gccagctggt cgatggcgcc ggcccggaag gtgtcggcgg cggccaaaag caccttctgc
       61 ccttgccggg caaagtggta ggcgagtttc ccgatggtgg tagtcttgcc ggtaccgttc
      121 acccccacta ccaggatgac gtgcggcttc tcttttgcgg ttttagcggt ggcaacgcct
      181 gaaccattca ccaggcagtg ccgcatttcg tctttcaaaa gactgatcac ggcgtcggca
      241 ccggtcaggt tattctgttg ggcccgcttt ttcaactgct cgactaaacg caaggtgacc
      301 gccacgccga cgtcggagcg gatgagggtg tcttccaagt cctggagcaa gccagcgtca
      361 atcctcggat ggagcccgat ggccgctgtg atcttgctta agaaagtatc tttggtctta
      421 gcgagactcg atttcaatcg gtcgagtgag aattgcatca gttttcccta tcacctatga
      481 ccaattacct ggttcacctt tgacggccac gaaggcctat gctaccaaag ttgcaacgag
      541 ctgcccgatg tccacaatcg ctttccgagt gggcgaagcg ccccccctgc gcgactgaag
      601 tcgcgcctcc caacttgaat ctgattctcc ccaactccca aatcccattt cctaattccg
      661 tctttcccta tcaccaatca cctacgacct cgcacctgcc ttacgccatc tccggcacca
      721 gttcggagag ccgcaccgag acaatcctgg aaacccccgg ctcttccatc gtgaccccgt
      781 agagcacgtc ggctgcttcc atcgtcttct tgttgtgggt gatgacaatg aactgggtgt
      841 tccgggcaaa gcgcttgatc agtttcagga agcggtcgat gttggcgtcg tccagggggg
      901 catccacttc gtcgaggatg cagaaggggc tcggcttgac taaatagagc gcaaagagga
      961 gtgcaatggc ggtgagagcc cgctcgccgc cggagagttg cgtaatggtc acgagttttt
     1021 tgcctttggg ccgggcgaaa acggcgatat ccatctcgag ggggtcgtcg gtgccggtca
     1081 gcgtgacgtc ggcttctccc ccttcgaaca gttcggcgaa aacggcctgg aaattatccc
     1141 gcacctgtcg gaaagtggtg ttgaactgct ccagcgcctg ccggtttagc tcttcgatcg
     1201 tggtctgcag ctgctcccgc gccttttgca ggtcggccat ttgctgcgca aggaattcga
     1261 accgtttttt ctgagattcg tattcttcca gcgccagaag attcaccggc cccagggaat
     1321 ccagccccgt gcgcagctcc tgcaattgac cgggaagatc ctccaaatga gccgcttctt
     1381 cgtccgacac cggtgccaaa gcctcgggcg aaatgcctga ctccgcctgc aggcgggagg
     1441 tgatctgctg caactgccct ttcaattccg tcaaggccag ttccacctgg tgctggcgtg
     1501 ccacggcttc atcgcggctc atgcgctgcc cctttacttt cccttccact agggcccgct
     1561 tctgccgcag ggagtctaac cgctgttcct ctgcggcggc cgccgaacgc aagacttcct
     1621 cttttgccaa cctgccggcc tgttgatctt caaacgaagt tccagaagtt tgctcccggc
     1681 tggttagggc cgccaactcc agccggtgcg aggttttttc ggcttggctc ttgcggatct
     1741 cggcaacgag ttcaccgagc cgccggctat tttcctgtgc gtgctcgaac tggccctgga
     1801 gcgagaccag ttccagccgg gcgtcaccga ttttttgcac ctgctcggat tcggctgact
     1861 ccagacgctc aatttctttg gccagttgtt cggtctccag ccggactccc ccccgctcct
     1921 cctgcagggc caaaaggtcg gctttttctt cttttccagc cgtctctccc cgggctaaat
     1981 gggcctcgat ctcctttcgc tcttgggcga gcaggagaat ttgctggtgc tgcgcctcgg
     2041 attctatctg gaagcgttcg tgctccagcg tttgctcgtg gagaagcgac cgattccttt
     2101 ccatttctct cttttgggtt tcgcttttgg cagccaaagc ggtcacctgt ttcccgaggc
     2161 tctccagtct gcgggcagat tcccggatct gcttttcccg gaccgctatt tcctcttccc
     2221 gtgcggccag ccgggccgcc cggccgacca aaccggtttc ccggtccccg ccgccggaga
//
LOCUS       DBNGZA010000389         2771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1668505, whole genome shotgun sequence.
ACCESSION   DBNGZA010000389 DBNGZA010000000
VERSION     DBNGZA010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1668505"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="AC16FU_08415"
     CDS             <1..640
                     /locus_tag="AC16FU_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994429.1"
                     /translation="LIFRDDQIDSLTIKALSTSPRVVLHEIWDENYEIIDKLLSEKTK
                     KTSKGKKPIRNEEFEIAVSLLLYLCGFSSGHYGTIAEYGEGVDILAFSSNDKFLLAGE
                     CTVEFPDKKGKLQNFYKRVQELKDHTDLEVVPVLFTRLPLNKIPLDEKNRAGQNKISI
                     LSNERIQRLLSMARKGSSVKEYHEFFKNPEPLDLEYISAKGVQLSFRHSRGF"
     gene            687..2654
                     /locus_tag="AC16FU_08420"
     CDS             687..2654
                     /locus_tag="AC16FU_08420"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR2994430.1"
                     /translation="MPMNLTQKLISSHLVNGGADPSTLAQGGGSIPSTTKPGEEIAIK
                     IDQTLLQDATGTMAMLEFEALGVPRIKVKRALVYVDHNMLQTGFENPDDHLFLQTMAA
                     KYGAIFSRPGNGISHQAGLERFDAPGETLLGADSHTPQAGALAMLAIGAGGFEVAAAM
                     AGEPYRFRMPEIVLVRLSGALKPWCSAKDVILELLRRFTVKGGVGKIFEYGGPGLAAL
                     TVPARSTIANMGAELGLTSSVFPSDEQAQRFLKAQKREKDFKPLVADDGAKYDDVVEL
                     DLSKLEPLIAQPKSPDKVVPVREIEGTEVKQVAVGSSVNSSFVDLMTAAKIMEGKKVH
                     PGLHMTISPGSRQVLIMATEAGGMAALFRAGARGLEVACGPCIGMGMAPPSKGKSVRT
                     FNRNFPGRSGTADDEVYLTSPEVAAATALNGVITDPRKLGTPPKIEMPEEFVIDDSGF
                     LPPAPDPGKVEIVRGPNIQRLPIPPQLDDAIKGVVLIKLKDNISTDEILPAGGKILPL
                     RSNIPAIAEYVFHYVDPDFPARAKAKGPGIIVAGDNYGQGSSREHAALAPMFLGVRAV
                     VAKGFARIHQDNLVNFGLIPLRFTKPEDYDALAQDDEVTLLNIRDCLKDKRPILLRNV
                     TRKREMLLEYKLTPRQIEIILAGGLVHYLKR"
BASE COUNT          654 a          698 c          829 g          590 t
ORIGIN      
        1 tcttattttt agagatgacc aaatcgattc cctaacaata aaggcactgt caacaagccc
       61 tagagtagta ttacatgaaa tatgggacga gaattatgaa atcattgaca aactcttatc
      121 tgaaaaaacc aagaaaacat ccaaaggtaa aaagcctata agaaacgaag aatttgaaat
      181 agcggtaagt ttgttgctct acttatgcgg attttccagt ggtcactacg gtacgatagc
      241 agaatatggt gaaggagtcg atatactcgc attttctagt aatgataaat ttttattggc
      301 cggtgaatgc accgttgaat ttcctgataa aaaggggaaa ctgcaaaatt tttacaagcg
      361 ggtccaagaa cttaaagacc atactgactt ggaagtagtc ccggtacttt ttactcgatt
      421 acctcttaat aagatacctt tggacgaaaa gaaccgtgct gggcaaaaca agatttctat
      481 cttatccaat gagagaattc aaaggttact ttccatggca cgaaaaggat cttctgttaa
      541 agaataccac gaatttttta agaaccccga gccactcgac ttagagtata taagtgcaaa
      601 aggcgttcag ttgagttttc gtcatagtcg aggtttctaa aagacttttc tgttaaaggt
      661 tatagaaaac gtgagggaaa actgacatgc ccatgaacct gacgcagaag ttgatctcct
      721 cccatttagt taatggcggg gccgaccctt cgactttagc tcagggtggt ggctcgattc
      781 cgagcaccac caagcccggc gaggagattg ccatcaaaat cgaccagacg ctgttgcagg
      841 atgccaccgg caccatggcg atgctggagt tcgaggcctt gggcgtgccg cggattaagg
      901 tgaagcgggc tttagtctac gtggaccaca acatgctcca gaccggcttt gagaacccgg
      961 acgaccacct ctttttgcag acgatggcgg ctaagtacgg ggcgattttc tcccggccgg
     1021 ggaacggcat ctcgcaccag gcgggcttgg agcggttcga cgcgccgggc gaaacgcttt
     1081 tgggggccga cagccacacg ccgcaggccg gggctttggc gatgcttgcc atcggcgcgg
     1141 gcgggttcga ggtggcggcg gcgatggccg gggagccgta ccggttcaga atgccggaaa
     1201 tcgttttggt gcgcctctcc ggcgcgctca agccctggtg ctcggccaag gatgttattt
     1261 tggaactttt gcggcggttt accgtcaaag gcggcgtggg gaagattttt gaatacggcg
     1321 gcccgggact ggcggcgctc accgtgccgg cgcggtcaac catcgccaat atgggtgcgg
     1381 aattggggct gacgagttcg gttttcccgt cggatgagca ggcgcagcgg ttcctcaaag
     1441 cgcagaagcg ggagaaagat ttcaagccgc tcgtcgccga cgatggggcc aaatacgacg
     1501 acgtggtgga gttggatctc tcgaaacttg agccattgat cgcccagccg aaaagtccgg
     1561 acaaggtcgt gccagtgcgg gagattgaag gcacggaggt caagcaggtg gcggtgggga
     1621 gttcggtcaa ttcgtccttc gtcgatttga tgacggcggc aaaaatcatg gaggggaaga
     1681 aagtccaccc tggcctgcac atgacgattt cgcccggctc gcgtcaggtt ttgattatgg
     1741 cgactgaagc gggcgggatg gcggcgctct ttcgggccgg cgcgcgcggg ctggaggtcg
     1801 cctgcgggcc ctgcatcggg atggggatgg ctccgccttc caagggcaag tcggtgcgga
     1861 ctttcaaccg caacttcccc ggccgaagcg gaacggcgga tgacgaagtc tatttgacca
     1921 gcccggaagt ggcggcggcg acggctttga acggcgttat caccgatccc cggaaactcg
     1981 gcacgccgcc caaaatcgaa atgccggagg aatttgtgat cgacgactcc ggtttcctcc
     2041 cccctgcgcc cgatccgggc aaagtcgaaa tcgtccgcgg gccgaacatc cagcgtttgc
     2101 ccatcccgcc gcagttggac gacgcaatca aaggcgtcgt gctcataaaa ctcaaagaca
     2161 acatctccac cgacgagatt ctcccggcgg gcgggaagat tctcccgctg cgttcgaaca
     2221 tcccggccat tgccgagtat gttttccatt acgtcgatcc cgatttcccg gcgcgggcga
     2281 aagcgaaagg cccggggatt attgtggcgg gcgacaacta cggccagggc tcgagccggg
     2341 agcacgcggc cctggcgccg atgttcttgg gtgtgcgggc ggtggtggcc aaggggtttg
     2401 cccgcataca tcaggacaat ttggtgaact tcgggctgat cccgctccgc ttcaccaagc
     2461 cggaagacta cgacgcgttg gctcaggatg atgaggtcac cctgctcaac atccgtgact
     2521 gcctgaagga caaacgcccc attctcctgc gaaatgtgac caggaagcgg gagatgcttc
     2581 tggaatacaa actgacgccg aggcagattg aaatcatttt agcgggcggg ctggtgcatt
     2641 atttgaaaag gtgaagcgga gtgctgaccc cgccctcgcc cgccccacaa ggggagggaa
     2701 attgcaaggg aaaggtgttg tcgtgtaggg gcgaatcttt acctgtcctg agcgaagtcg
     2761 aaggagattc g
//
LOCUS       DBNGZA010000390         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2515691, whole genome shotgun sequence.
ACCESSION   DBNGZA010000390 DBNGZA010000000
VERSION     DBNGZA010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2515691"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC16FU_08425"
     CDS             <1..786
                     /locus_tag="AC16FU_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR2994431.1"
                     /translation="PFTPPPAGHIAGRVTDDSTGLPIADAVVGAVAFDSTHRMPIIRF
                     THTGEDGSYRIESLPSIPFFVIAWAHGYLAELYDNAHRFEDATRVIPPADGIDFALTL
                     KPMGPPAVSGIVMNQNLGTPLAGVLVNAVTPQGEVAGSAVSLPDGSFLVEGLGPGQFT
                     LEARSNLAPPVAVGPFDLSGDGQSGIVVNVPAAVANVRGDVNRDGLIDVVDIVGEIDA
                     VVFGRLLDPLLADVNCDGVVDIVDIVGLVQFVVFGRPSPCALN"
     gene            1004..>2108
                     /gene="lpdA"
                     /locus_tag="AC16FU_08430"
     CDS             1004..>2108
                     /gene="lpdA"
                     /locus_tag="AC16FU_08430"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR2994432.1"
                     /translation="MPEISCDIFVIGSGPGGYVAAIRAAQLGNKVAIAEKDSWGGVCL
                     NWGCIPTKALLFTSELFHLIEIAKEYGIQTSVSGFSLDTLRAKKERVVKQLVGGVEFL
                     LKGNQVTALKGTARFLNPRQIEVSSDGQKSLVTARKTIIATGTEPVKLPMLPVDGDLV
                     IDSQDALELKRIPKKIVIIGGGAIGIEFADIYRAFGSQVVVVEMLDSILPTLDREVAG
                     TLANILRKRGIEIYTSHKVTQTRLEKNKAILTVEPVAGAAPPLSIECDMVLVAVGLRG
                     QVSGFGLEQTGVLADKHGFITVNGRLETNVPDLYAIGDVVGGKLLAHKASHEGIIAAE
                     AASGRKVEMDYRVVPYAVFTDPEIGAVGLTEEEA"
BASE COUNT          423 a          603 c          649 g          433 t
ORIGIN      
        1 ccgttcacac cgccgcccgc gggtcacatt gccgggcggg taactgacga cagcaccggc
       61 ttgccgattg cggatgcggt ggttggtgca gtcgccttcg actctacgca ccggatgccg
      121 atcatccggt tcacgcacac cggagaggat ggctcctacc ggattgagtc cttgccttcc
      181 attccgtttt tcgtgatcgc ctgggcgcac ggctacctgg ccgaactcta cgacaatgcc
      241 caccgctttg aagacgccac ccgggttatc cccccggccg acggaattga ctttgcccta
      301 acgctcaaac ctatgggccc gccggcggtc tcgggcatcg tcatgaacca gaacctgggt
      361 acacccctgg caggcgttct ggtgaatgcc gtgacgcctc agggtgaggt cgctggttcg
      421 gccgtctccc taccggatgg cagtttcctg gtggagggcc ttggccccgg tcagtttacg
      481 ctcgaagccc gctctaacct ggcgccgcca gtggctgttg gtccttttga cctctccggc
      541 gacggtcaga gcgggatagt ggtgaacgtc ccggctgccg tggccaacgt gcgcggtgac
      601 gtcaaccgtg acgggctgat cgacgtggtg gacatcgtgg gcgaaatcga cgcagtcgtc
      661 ttcgggcggc tcctcgatcc gcttttggcc gacgtcaact gcgacggagt cgtggatatc
      721 gtcgacatcg tcgggctggt gcagttcgtg gtttttggcc gcccgagccc ctgtgcgctc
      781 aactgacgtt tgaccgttcg agaaaagccc cgcttcaagg cggggctttt ctattttggg
      841 gaatagaacc gccgcttcgg tcttcggcaa gctcgggcga aggagaaagc gttacagccg
      901 gtatactcac acgggagagg aattctcgga cattgggcgg gcaaaattga caagagccgg
      961 ttgtgattct atactcagcc ggacaataga gaaaggatag gccatgcctg aaatttcctg
     1021 tgacattttt gtgatcggtt ccggcccggg cggctacgtg gcagccatcc gcgccgctca
     1081 gttggggaac aaagtggcga ttgccgaaaa agattcctgg ggcggggtct gcttgaactg
     1141 gggctgcatc cccaccaagg cgcttctgtt cacgtccgaa ctcttccatt taatcgagat
     1201 tgccaaagag tacggcattc agaccagtgt ctccggattt tccttagata ccctccgtgc
     1261 gaagaaagaa agagtggtca agcagctggt gggtggagtt gagtttctgt tgaaagggaa
     1321 tcaggtcacc gcgctcaaag gaacggcccg atttctcaat ccccggcaaa ttgaagtctc
     1381 aagtgatgga cagaaatctc tcgtaactgc tcggaagacc atcatcgcca ccggtaccga
     1441 accggtgaag ctccccatgc tgccagtgga cggcgatctg gtgatcgaca gccaggatgc
     1501 gctggaactg aaacggatac ccaaaaaaat cgtcatcatc gggggcggcg cgatcggcat
     1561 tgaattcgcc gacatctacc gggccttcgg cagccaggtg gtcgtggtcg aaatgctgga
     1621 ctcgattctg ccgaccttgg accgggaagt ggccgggact ttggcgaaca tcctgcgcaa
     1681 gcggggaatc gaaatttata ccagccacaa agtcacccag acgagactgg agaagaataa
     1741 agcgattttg accgtcgaac cggtcgccgg ggccgcacca ccactttcga tcgagtgcga
     1801 tatggtgctg gtggcggtcg gcttacgagg gcaggtctcc ggtttcggtt tagaacaaac
     1861 cggcgtgctg gccgacaagc atggtttcat caccgtcaac ggccggctgg aaacgaacgt
     1921 cccggacctc tacgccatcg gcgacgtggt cggcgggaaa cttttggccc acaaggcttc
     1981 gcacgagggg attattgccg ccgaagccgc gtccggccgg aaagtcgaaa tggactaccg
     2041 ggtcgtccca tacgccgtct tcaccgatcc ggaaatcggg gcggtcggct tgaccgaaga
     2101 ggaagcgc
//
LOCUS       DBNGZA010000391         3113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2835346, whole genome shotgun sequence.
ACCESSION   DBNGZA010000391 DBNGZA010000000
VERSION     DBNGZA010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2835346"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..764)
                     /locus_tag="AC16FU_08435"
     CDS             complement(27..764)
                     /locus_tag="AC16FU_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994433.1"
                     /translation="MTLIVTLAVSAFLVVNAGAAVPQLINYQGKLTDNSGAPITSAVT
                     LKFRIYDAPAGGTLLWGPESHPVTPDANGDYNVILGAGTPPVAFPANLFAATPRYLGV
                     EVNTDGEMTPRQQITAVAYSLRVATVDGATGGTISGNTAIQSDLTVSGDVGIGTASPA
                     QKLDVAGTAQMTGFKLPTGASNGFVLTSNAVGVGTWLAAGAGSGWSLTGNSGTTPGTN
                     FLGTTDNQALELKVNGSRALRIEPNTT"
     gene            1329..2798
                     /locus_tag="AC16FU_08440"
     CDS             1329..2798
                     /locus_tag="AC16FU_08440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR2994434.1"
                     /translation="MSGGGKFQPGQKQSGATVITQRLQLFRRLALAGDLFLLFLVYLL
                     VVAFRESTAVQQLLNLQEYSPYRPMLLLILPIWGLVLALSPHCYELRAKRLWRMGLDL
                     GVVTLKGAGVLLAALFVLHAPPQSRFLVFLFCTTSWLALVTYRVTLTTALGWARRRGK
                     NLQKLIVVGTGRAAQKLVDQILTHPAWGLRISGFVYNGEDLSLWRFRDIPVIGTLFDL
                     PRIIQNNHIDWVVFAVPEGQLGLIGPSMQLCEEMGTNVCLLTDFFSLRFARKKAGELF
                     GQPVLLFSRAPEPDVRLFIKYCLDRLLAFLGLVLVAPIMACAAVVIKLASPGPVLFRQ
                     ERCGLNGKRFTLYKFRTMVPEAEALKSQLLDKNEMDGAAFKITNDPRLTPVGKVLRKY
                     SLDELPQLMNVLKGEMSLVGPRPPLPDEVSRFDRWQRRKLSMKPGLTCLWQVNGRNHV
                     NFEKWMRLDLEYIDNWSLWLDAKILAKTVPAVLSGRGAK"
     gene            2818..>3113
                     /locus_tag="AC16FU_08445"
     CDS             2818..>3113
                     /locus_tag="AC16FU_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994435.1"
                     /translation="MMKKALVIGFVFLLAPVISSGQVPLGASPYGWVYEYVEHLVAAG
                     YLSPAFKNSLPYQWDNLFDSLAVLQNSVESGTFTLPRGEQWLVDRLLQEQEVVSA"
BASE COUNT          622 a          873 c          915 g          703 t
ORIGIN      
        1 ccactttggc cgccaattaa gttaggttaa gtggtattcg gctctatgcg caaggcgcgg
       61 ctgccgttca ctttcaattc cagggcctga ttgtcggtag tgcccaaaaa gttggtgccc
      121 ggagttgtgc cactgttgcc ggtaagactc cagccacttc cagcgcccgc cgccagccag
      181 gtgccaacgc cgaccgcatt cgaagtcaaa acaaaaccat tggaagcgcc ggtcggcaat
      241 ttaaaaccgg tcatctgggc cgtgccagca acgtccaatt tctgggctgg actcgccgtc
      301 ccgatgccga cgtcgccgga gacggttaag tcactctgga tggccgtatt tcccgaaatg
      361 gttccgccgg tagctccatc aaccgttgcc acgcgcaggg aataggccac ggcggtgatc
      421 tgctggcggg gggtcatttc gccgtccgta ttcacctcca cgccaaggta gcggggagtg
      481 gccgcaaaca ggtttgccgg aaaagcgacc ggcggtgtac ctgcacccag aatgacgttg
      541 tagtcgccgt tggcgtccgg ggtgacaggg tgcgattcgg gtccccacaa aagcgttccg
      601 cctgccggag cgtcgtagat gcggaacttc aacgtgaccg ccgaagtaat cggcgcaccg
      661 gagttatcag tcaatttccc ctggtagttg atgagttggg ggacggcggc gcctgcgttc
      721 accaccaaaa aggcagatac agccagcgta acgataaggg tcatgagtgt cattcggatg
      781 gttcgactcc ctcgatttcg ctccctcaac ttcgttcggg acaagtcggg acaagtacgc
      841 tcaccattaa gacaggacat aagtccccct ttcatgtaaa gggtgttacg ggcgaacagc
      901 ggacggatgg tcatgatttg ccggagatga aaacggtttg cgttacacat acacgttcct
      961 cctcccgtgc cacaacggca caggtaacaa aaacgttggg cgaggtcagg aaacctcgca
     1021 ctaccacagt atcttctgag cacccttaag ccctgccctt gacccggttt gaaggtatcc
     1081 tcccccacga ttttgtcaag ccaaaattag tcctgaattg aaaaaatccc cacgggcggg
     1141 atgcaaaggc cggccagtcc agcgtctttc tccgtcgatt cgttgctccg cctgagagaa
     1201 ggtcgtttcg atgccgggca ggaggttctg tgccccttta gtcttccatc ttcttgccga
     1261 ataagggaat aggaagcttc agggagtgag gcaatgagag aaatggtatt tcaagcaagc
     1321 ggaactgctt gtccggcgga ggcaaattcc agccgggaca gaaacagagc ggggcgactg
     1381 tgatcacgca aaggttacag ctctttcgcc gtctcgccct ggcgggcgat cttttcctct
     1441 tgtttctggt gtacctcttg gtggtggcct ttcgtgaatc tactgccgtt cagcaactgc
     1501 taaatcttca ggagtacagt ccctaccggc cgatgctttt gctcatcctg ccgatctggg
     1561 ggttggtttt ggccttgtcg ccgcactgct acgagctgcg ggccaagcga ctctggcgga
     1621 tggggctgga cctgggtgtg gtaacgctca agggagcagg tgtcctgctc gctgcacttt
     1681 tcgttttgca tgcgccgccg caaagccgct ttttggtatt tctcttctgt accaccagtt
     1741 ggttggccct ggtaacctac cgcgtgaccc tgacaactgc cttggggtgg gcgcggaggc
     1801 gcggaaagaa tctccaaaaa ttgatcgtcg tcggtaccgg acgggcggcg cagaaattgg
     1861 tggaccagat cctcacccat ccggcctggg ggctgcgcat ttcgggattc gtctacaacg
     1921 gggaagacct ctcgttgtgg cgcttccgtg acattccggt catcggcacc ctctttgacc
     1981 tgccccgtat cattcagaac aaccacatcg actgggtggt cttcgcggtg ccggaggggc
     2041 agttgggatt gatcggcccc agcatgcagc tctgcgaaga gatggggacg aacgtctgtc
     2101 ttttgaccga ttttttctcc ctgcgcttcg cccgcaaaaa agcgggggaa ctgttcggcc
     2161 agccggtgct cctcttctcc cgggcgcccg aaccggacgt tcggctcttc atcaagtact
     2221 gcctcgaccg gctcctcgct tttctggggt tggtattggt ggctccgatt atggcctgcg
     2281 cggcggttgt catcaagctt gcgtctcccg ggccggtgct cttccggcag gagcgctgcg
     2341 ggttgaacgg caagcgcttt actctctaca aattccgcac catggtgccg gaggccgagg
     2401 cgttgaaaag tcagcttttg gataagaacg aaatggacgg agcggctttc aaaatcacca
     2461 acgacccacg gctcaccccg gtgggaaaag tgctgcggaa atattccctc gacgaactgc
     2521 cccagttgat gaacgtcctg aaaggggaga tgtctttggt cgggccgcgg ccgcctttgc
     2581 cggatgaagt cagccgtttc gaccgctggc agaggcgcaa actctccatg aagccggggc
     2641 tcacctgcct gtggcaggtg aacggccgga atcacgtcaa tttcgagaag tggatgcggt
     2701 tagatttgga gtacatcgac aactggtcgc tctggctgga tgccaaaatc ctggccaaga
     2761 cggttcccgc agtgctctcc ggccggggag cgaagtaaga atacgtcgag tcagcggatg
     2821 atgaaaaaag cactggttat tggtttcgtt tttctactcg caccggttat ttcctccggg
     2881 caagtccctt tgggcgcgtc accctatggc tgggtctacg agtacgtcga acacctggtc
     2941 gccgccggct acctctcccc ggcgttcaaa aacagtttgc cgtaccagtg ggacaacctg
     3001 ttcgattcac tggcagttct ccagaacagt gtggaaagtg gaactttcac tttacccagg
     3061 ggggaacaat ggctggtgga ccgtctgctc caggagcagg aagtggtttc ggc
//
LOCUS       DBNGZA010000392         9227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1351685, whole genome shotgun sequence.
ACCESSION   DBNGZA010000392 DBNGZA010000000
VERSION     DBNGZA010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9227
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1351685"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="AC16FU_08450"
     CDS             complement(<1..409)
                     /locus_tag="AC16FU_08450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR2994436.1"
                     /translation="MLPHLQKSFPREQLLSDALARSCYASDASIYAVTPQAVLLVKSE
                     ADVARAIDFANKHRISLTPRGAGSGLAGGALGEGLILDFSGYRGIVSLDSESKTVTVR
                     VGTIYEELNAFLKPHRLVFAPDPSSGDVCQIGGM"
     gene            complement(482..1711)
                     /locus_tag="AC16FU_08455"
     CDS             complement(482..1711)
                     /locus_tag="AC16FU_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994437.1"
                     /translation="MSLLDLFKQVYVSLRANRMRSALTMFGIVWGIASVIILVGIGDG
                     FKAANQREFTQLGSNISILWGGRTSMQAGGRRGGKDIRFTEDDVKAVIAQASLVHQAS
                     PEIRRDLQIEYGPKSMATDVHGVYSAFGQMRSLVLESGRFLNQADFDVKRAFCVIGDE
                     VNRRLFSGENPVGKMIRIANRPFLVIGKLQKKDMGSRYGSPDDEQIFIPFSVAQQIFS
                     RKYINNIVISPRNPDEDEAALAEVKRILSRRLNFNPEDRDAVNIWNTARTARQVNTLF
                     FAIEILMTLIGGVTLGVGGVGVMNIMLVSVTERTREIGIRRALGARRRNVLFQFLSEA
                     LVLTFSSGLLGMAVGIGLTKLIASLPLPEGFDPPVVSPTVVVTSIILLTVVGVLSGTY
                     PALRASRLSPVEALRYE"
     gene            complement(1711..2850)
                     /locus_tag="AC16FU_08460"
     CDS             complement(1711..2850)
                     /locus_tag="AC16FU_08460"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994438.1"
                     /translation="MKTIRSYAFVLWPLVALLLACGGGKKQKVVTVTVERGDITQSVV
                     ATGKLEPVVKIEIRAKASGIVRKLYVEEGDRVRAGQPLVEIAPQATPVELVRAREAVN
                     SAALDLELKKLDWQKADSLYKRKLISQDESRRFRTQFDLAQSRHTAAAAELAILEQSE
                     AGPNDTSHRMAQGADLQEEDENAEVLRSTVVYAPFSGVVLSKNIDVGSSVSAVSAASG
                     GSVVMTVADDSRMWFKGEIDESDLGKIKLAMTAKIILDAYPDTFFTGILDWISPMGTE
                     KDNLTTFQVRATLKNSTGRLKAGMRGKGEIVLAEKKDVLWANEGAIIYNEGKTFVDLF
                     DVKNPQKPKRQPIKTGISDGLKTEILEGVKEKDTIIMPSGPSSEL"
     gene            2992..4857
                     /locus_tag="AC16FU_08465"
     CDS             2992..4857
                     /locus_tag="AC16FU_08465"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR2994439.1"
                     /translation="MAHPFLERLTYGPILCDGAMGTLLYQRGIPYERCFDEVSLSKPD
                     VVSQIHRDYIAAGAEVIETNTFGANRFRLATHGLEDRVVKVNRAGVKVARDAREISGK
                     PVFVAGSIGPVGQPLTPVGRITPQQAFDAFKEQAEGLLEGGVDLFIVETFSDLTEITQ
                     AVKAVRSVCDLPIVAQMSFTEEGATLAGQSPEAVAQALEALGVDMLGTNCSVGPQALL
                     DVLLAMRRTTKRPLSIQPNAGLPRLVEGRWFYYTSPTYFAEYAQKFLEAGATLVGGCC
                     GTTPEHIHAMAQILKRFRATATEVHPAPEAPRVQVVEKPEPLPAFEASHFAQKLGKKF
                     VVSVELDPPRGINPEKLLKGAELMEKHGVDAINIGDSPMARVRMSCIAIGVLIQKRVK
                     NIDIILHWTTRDRNLMGLQSDLIGAHALGIRNILAITGDPPTIGDYPHATGVYDVDSI
                     GLIQVIARLNTGVDWAGNSIGSPTALTIGCGVNPTAEDMAKEVERYKKKIEGGAQFAF
                     TQPLYELSILEEFLDRTHDCRIPVLLGLLPLQSTRHTEFLHNEVPGITIPADIRKRMA
                     EAGHNGAQEGIAMCRDLLEKAKDMVSGAYLMPSFGRYETVLEVVKGFLTKTQVVT"
     gene            4971..6476
                     /locus_tag="AC16FU_08470"
     CDS             4971..6476
                     /locus_tag="AC16FU_08470"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR2994440.1"
                     /translation="MEIQVLKGEIQDAKDEAIVVNLFEGVKAPGGATAAVDQKINKSI
                     TKLIATGDFTGKKNQVAVLYPPDGFPAKRVFVVGLGKKEKFNLDVIRQAAGSVCQKAQ
                     QLNVKSYTTIVHGAGAGRISPDDAAQALVEGTLLANYRFDRYHTETDSRPQPVQKVTL
                     LERDGRNIVRMKKGAAIGEIISWAQCLARDLSNEPGNVLTPTKLAETAADLGKKYGFK
                     TQILRKAEIEKLKFGALLGVARGSAEPPTFTVMEYQPSKRAFGPIVLVGKGVTFDSGG
                     ISIKPADNMDEMKGDMTGGAVVIATITAAARLQLPVHLIGLVPSTENLPSGTSYKPGD
                     VLRSYLGKTIEIINTDAEGRLILADALAYAQTFKPKAVIDVATLTGAVKIALGTVAAG
                     LLASDQKLANRLKAAADKSAEKVWQLPLWEEYQERIKSDIADVKNSGGRPGSTITAAC
                     FLQKFVGDSPWAHLDIAAMDIEEKGQPYVPKGGTGFGCRLLLQLLRDWGRG"
     gene            6642..7391
                     /gene="sfsA"
                     /locus_tag="AC16FU_08475"
     CDS             6642..7391
                     /gene="sfsA"
                     /locus_tag="AC16FU_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR2994441.1"
                     /translation="MRFEKRLLRARFIARPNRFTVKAWLQGKPILAHLHDPGRLKELL
                     FSGAKLLLEPTKGDRKTNFDVRLVETKEKNLVSLYSTYPNLLLSESWNLPLVKKIFGP
                     VSVLPEKQLGKSRIDFLLQNGKQRIWVEVKSVTLVENGVALFPDAPTARGRRHLEELI
                     QAKKSGDRAVVLFVAQRSDAKVVMPNVRTDPAFARTLARALKAGVEMYAFRCRIDRRG
                     IVLDRAIPIRLPDRRNFLATAGVTKITSKTE"
     gene            7394..7879
                     /locus_tag="AC16FU_08480"
     CDS             7394..7879
                     /locus_tag="AC16FU_08480"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR2994442.1"
                     /translation="MADQVEKSPEVMSRRSFFATLTKLSGGFIAVLLGIPIVGNIISP
                     FFQKPETDWIDLGNADDLHGMAPLKIPYSVDVQDGWYKTKADRFVYVVWRNDDFLVLS
                     PTCSHLGCSVRWDGRKFACPCHGGTYDREGKVTGGPPPKPLAHFATKIESGRLMAKEV
                     V"
     gene            7956..>9227
                     /locus_tag="AC16FU_08485"
     CDS             7956..>9227
                     /locus_tag="AC16FU_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR2994443.1"
                     /translation="MKRITSWLDKRFGLGWLIKKEWYKPLPPSTNWLFSLGSVALFLF
                     VVQILTGIFLALYYVPTPDHAYESVQFINEKVIFGKLLRGIHSWGAGLLMIILPLHLL
                     RVFFYGAYKKPRETTWMAGVLLLGVTFTFAFTGYLLPWDEKGFWATVVGTNIAGSPPV
                     LGKYVATWLKGGVTFGALTLSRFYAIHVLLLPATFLVLVSVHLYLVLKHNTAGHWDPQ
                     KNDFSRGFPFYPIQVGRDALAALVVLAIVGVLAFVKPPHLLDVANPSNTSFIPRPEWY
                     FLFHFQLLKYFPGSSRVIATVILPTLFFLALILLPFYDRRPRRSPLKRPVALTLASLT
                     VVSVITLTVLAIQEDVRLSGFGPKVPLNAVQKAGLEVFNTYGCKMCHGEGGYPGGQGP
                     SLIDATTRHDQTWLMQHFKDPQSLVPGSKMPK"
BASE COUNT         1969 a         2814 c         2513 g         1931 t
ORIGIN      
        1 gcatccctcc aatctggcac acatcgccgg atgagggatc gggtgcaaac accagccggt
       61 gcggcttcag gaacgcgttc agctcttcat agattgtccc cacccgcacg gttacggttt
      121 tgctttcgct atcgagactg acaattcctc gatagccaga aaaatcgagt attaagccct
      181 cccctaacgc gcccccggcc agacccgatc ccgcaccgcg cggcgtgagt gaaattcggt
      241 gcttattggc aaaatcaatg gcgcgggcaa catccgcctc ggatttaacg aggagcaccg
      301 cctgaggtgt taccgcataa atactggcat ccgaggcgta gcaggagcgg gccagggcgt
      361 cggagagcaa ctgttctcga ggaaaacttt tctggagatg gggcagcatt gcaccagaat
      421 atataggcag gtaaaacgaa aaatagcaaa taatcggaga cgggaacttc cggagccgga
      481 gctactcgta ccgcagcgct tcgaccggcg aaagccggga agctcgcaaa gccgggtagg
      541 tcccggataa gacaccgaca accgtcaaga ggataatgga agttaccacc accgtcgggg
      601 aaaccaccgg cgggtcgaac ccctccggca gaggcagact cgcaatgagc ttggtcaggc
      661 cgatgccgac cgccatgccc aaaagcccgg aagagaacgt caaaacgagc gcctccgaca
      721 aaaactgaaa gagcacgttc ctgcgcctgg ctcccaaggc acggcggatg ccgatttccc
      781 gggtgcgctc ggtcacggat accagcatga tgttcatcac ccccacacca ccgactccca
      841 aagtgacccc gccgattaaa gtcatcaaaa tctcaatggc gaagaaaagt gtgttcacct
      901 gccgggcggt gcgggcggtg ttccagatgt tcaccgcatc ccggtcttcc ggattgaaat
      961 tgagccgccg gctcaaaatc cgcttcactt cggccaaggc ggcttcgtcc tcgtccgggt
     1021 tccgcggcga gataacgatg ttgttgatgt acttccgcga aaaaatctgc tgcgccaccg
     1081 aaaagggaat gaaaatctgc tcgtcgtccg gactgccgta acgggaaccc atgtctttct
     1141 tctgcagttt gccgatcacc agaaacggtc ggtttgcaat ccggatcatt ttccccactg
     1201 gattttctcc ggagaagagc cggcggttga cctcgtcgcc gatgacgcaa aaggcccgtt
     1261 ttacgtcgaa gtcagcttgg ttcaaaaagc ggcccgattc gagcaccaga ctgcgcatct
     1321 ggccaaaggc ggaataaacc ccgtgcacgt cggtggccat cgatttgggg ccgtattcaa
     1381 tttgtaaatc gcgccggatt tccggggatg cttggtggac taaactggcc tgggcaatga
     1441 ccgctttgac gtcgtcctcg gtgaagcgga tatccttccc gccacgccgg ccgcccgcct
     1501 gcatagaggt tctcccgccc cagaggatgg agatgttaga acctaactgg gtaaactccc
     1561 gctgattggc ggctttgaag ccgtcgccga tcccgaccaa gatgatgact gaggcgattc
     1621 cccagacgat gccgaacatc gtcaaagccg agcgcatccg gttggcgcgc aggctgacgt
     1681 aaacctgttt gaataaatcc aacaacgaca tcagagttca ctggacgggc cggagggcat
     1741 gataatggtg tctttctctt tgaccccttc taaaatttcg gtcttcagac cgtcggagat
     1801 gccggttttg atcggctggc gttttggctt ttgcggattt tttacgtcga agagatcaac
     1861 gaacgtcttg ccctcgttgt agataatcgc cccctcattg gcccagagga cgtctttctt
     1921 ttccgccagg acgatttccc ccttgccccg catccccgcc ttcagccgtc cggtcgagtt
     1981 cttcaaggtc gcccgcacct ggaatgtggt caggttgtcc ttttcggtcc ccataggaga
     2041 aatccagtcc aaaatgccgg tgaaaaaggt gtccgggtag gcatccagaa tgattttggc
     2101 ggtcatcgcg agtttgattt ttcccaaatc ggactcgtca atttccccct tgaaccacat
     2161 ccgcgaatcg tcggcgaccg tcatcaccac gctgccgccg gaagcggcgg agacggccga
     2221 gaccgaggaa ccgacgtcaa tgtttttcga cagcaccacg ccggaaaagg gggcgtagac
     2281 caccgtgctt ctcagcactt cggcgttttc atcttcctcc tggagatcgg caccctgggc
     2341 catacggtgg ctcgtgtcgt tgggtccggc ctccgactgc tccaaaatcg ccagttcggc
     2401 ggctgcagcg gtatggcggg attgcgctaa gtcaaactga gtcctaaacc ggcgggattc
     2461 atcctgcgaa atcaatttcc ttttgtagag ggaatcggct ttctgccagt ccaatttctt
     2521 gagttccagg tcgagcgctg cggagttcac cgcctcgcgc gccctcacta attccaccgg
     2581 cgtggcctgc ggcgcgattt ccacgagcgg ctggcccgcg cggacccggt cgccttcctc
     2641 gacgtacagt ttgcggacga tgccggaggc cttggcgcgg atctcgattt tcaccactgg
     2701 ctccaacttg ccggtagcga caaccgattg ggtgatatcc ccccgttcca ccgtgaccgt
     2761 caccaccttt tgctttttcc ccccgccgca ggcgagcaaa agagccacca gcggccagag
     2821 gacgaatgcg taactccgga tagtcttcat ttcccttacc gttgcttacg gggaaaaagg
     2881 aagagaggtt gcaggaaaac ctctctcttg gccgctggct actggaaact ggctacggct
     2941 acttgcttcc gccattttac tcttccacgc tttttcccta aattccagac aatggctcac
     3001 ccctttctcg aacgcctgac gtacggcccc atcctctgcg acggcgccat gggcacgctc
     3061 ctctaccagc ggggcatccc ctacgagcgt tgtttcgacg aggtctccct ctccaagccc
     3121 gacgtagtct cccagattca ccgcgactac atcgccgccg gcgcggaggt gattgagacc
     3181 aataccttcg gggcgaaccg cttccgcctc gccacgcacg ggctggaaga ccgggtggtc
     3241 aaggtcaacc gtgccggagt caaagtggcg cgcgatgccc gcgagatctc cggcaaaccg
     3301 gtcttcgtgg ccggttcaat cggccccgta ggtcagccgt tgacacctgt cgggcgaatc
     3361 acgccgcagc aggcgtttga cgccttcaaa gaacaggcgg aaggattgct cgaaggcggg
     3421 gttgacctct tcatcgttga aaccttctcc gacctcaccg agatcacgca agccgtgaaa
     3481 gctgtccgtt ccgtttgcga cctgcccatc gtggcgcaga tgagctttac cgaggaaggc
     3541 gcgacgctcg cggggcagtc gcccgaagcg gtggcgcagg cgttagaggc cctcggcgtt
     3601 gatatgctcg gcaccaactg cagcgtcggc ccgcaggcct tgctggatgt gcttctggcg
     3661 atgcgacgga cgacgaagag gccattgtcg attcagccca acgccggtct tccccgatta
     3721 gttgaaggac gctggttcta ctacacctcg cccacctact tcgccgagta tgctcaaaaa
     3781 tttttggaag ccggcgcgac gcttgtcggc ggctgttgcg gcaccacgcc ggagcacatc
     3841 cacgccatgg cgcaaattct caagcgcttc cgggctactg cgacggaagt ccatccggct
     3901 ccagaagcgc cccgcgttca ggtcgtcgaa aaaccggagc cgctgcccgc atttgaggcc
     3961 tcccacttcg cgcagaagtt gggtaaaaag ttcgtcgttt cggtggaact cgatcccccg
     4021 cgcggcatca atccggagaa actactcaag ggcgccgaac tgatggaaaa acacggcgtt
     4081 gatgccatca atatcggcga ctcccctatg gcccgcgtgc ggatgtcctg catcgccatc
     4141 ggggtcttga ttcaaaagcg cgtgaaaaac atcgacatca tcctccactg gacgacgcga
     4201 gaccgcaacc tgatgggcct gcagtccgac ctgatcggcg cgcacgctct ggggattcgg
     4261 aacatcttgg ccatcaccgg cgaccctccc accatcggcg actacccgca tgccacggga
     4321 gtttacgacg tagattccat cggcttgatt caggtgatcg cccggctcaa caccggcgtc
     4381 gactgggccg gcaactccat cggcagcccc accgctttga ccattggctg cggagtcaat
     4441 cccaccgccg aggatatggc taaggaagtg gagcggtaca agaagaaaat cgagggcggc
     4501 gcgcagttcg ctttcaccca gcccttgtac gaactttcta ttctggagga attcctcgac
     4561 cgcacccacg attgccggat tccggtgctt ttaggtcttt tgcccttgca gagcacccgc
     4621 catactgagt ttttgcacaa cgaggtgccg gggattacga ttccggctga tattcgcaag
     4681 cgcatggccg aagccggcca caacggcgcg caggaaggga ttgcgatgtg ccgcgacctt
     4741 ctggaaaagg cgaaggatat ggtttccggt gcttacctga tgccttcctt cgggcgctac
     4801 gagacggtgt tagaagtggt gaaagggttt ttgaccaaaa cgcaggtggt aacgtaaaaa
     4861 caatcgtgcc tcgggaggcg cggctttagc cgcgcatgac gacccatggc gtgcgactga
     4921 agtcgcacct cccggtattg ggtcccagag gtagattgca aggagcaaac atggaaatcc
     4981 aagtgttgaa aggtgaaatt caagacgcta aagacgaagc catcgtcgtc aacctctttg
     5041 agggggttaa agcccccggc ggggccactg cggcggtcga tcagaagatc aacaagtcca
     5101 taacgaaact gatcgccacc ggcgatttca ccggcaagaa aaatcaggtc gccgtcctct
     5161 accctcccga tggttttccg gccaagcgag tcttcgtcgt tggcctggga aagaaagaaa
     5221 aatttaatct cgacgtcatc cgccaggccg ccggttcggt ctgccagaaa gcgcagcagt
     5281 tgaatgtgaa atcgtatacg accattgtgc acggcgccgg cgcgggacga atcagcccgg
     5341 atgatgccgc ccaagccctc gtcgaaggca cgcttttggc caactaccgc ttcgatcgct
     5401 atcataccga gaccgactcc cgccctcagc cggtacaaaa ggtcactctc ctcgaacggg
     5461 acggccggaa tatcgtgcgt atgaaaaaag gtgcggcaat tggcgagatt atctcctggg
     5521 cacagtgttt agcgcgcgac ctatcgaatg aaccgggaaa cgtcctcacg ccgaccaaac
     5581 tggccgaaac cgctgccgac ctcggcaaaa aatacggctt caaaactcag attctccgca
     5641 aagccgaaat tgaaaaactg aaattcggcg ccctgctcgg cgtggcccgc ggctcggctg
     5701 aaccgccgac cttcacggtt atggagtatc aaccttctaa gcgcgccttc ggcccgattg
     5761 tcttggtggg caagggcgtc accttcgact ccggcggcat ctcaatcaaa ccggctgata
     5821 acatggatga gatgaaaggc gatatgaccg gcggcgcggt cgtgattgct acaattaccg
     5881 ctgccgcgcg gttgcagttg ccggtgcatt tgattgggct ggttccgtcc accgaaaacc
     5941 tcccctccgg cacatcgtat aaaccgggtg acgttctgcg gtcgtattta ggaaaaacaa
     6001 ttgaaatcat caacaccgac gccgaaggac gattgatttt ggccgatgct ctggcctacg
     6061 cgcagacctt caagccgaaa gccgttatcg acgttgccac cttgactggc gcagtgaaaa
     6121 tagctttggg aacggtggcc gccggacttc tggccagcga tcagaaatta gccaaccggc
     6181 tcaaagccgc tgccgacaaa tcagccgaaa aggtgtggca gctgcctctc tgggaggagt
     6241 atcaggagcg gattaagtcg gacattgccg acgtgaaaaa ctccggcggc cgtccgggtt
     6301 cgaccatcac cgccgcctgc ttcttacaaa aatttgtcgg tgactccccc tgggcacatt
     6361 tagacatcgc cgctatggac atcgaggaaa aaggtcagcc ctacgtcccc aaaggcggca
     6421 ccggcttcgg ctgccgctta ctcttgcaac ttctgcgcga ctgggggcgg gggtaacatc
     6481 ttcgctctgg ccgctatctc ttccctcccc ttgaggggag ggccagggag gggtcatatt
     6541 ccttctttct ttgaccaccg ccactgtcag gttccttaca aactgagcca actctcgctc
     6601 gtattctttc caaaaccggg gaattggttt cgagagatag aatgagattt gaaaaacgcc
     6661 tccttcgagc aaggttcatt gcccgcccca atcgatttac tgtcaaagca tggctgcaag
     6721 gcaaaccgat tctggcccac ctccacgacc ccggacgcct gaaagaactc ctcttttccg
     6781 gagcaaaact tctcctcgaa ccgaccaaag gtgaccggaa gacaaatttc gatgttcgtc
     6841 tggtcgaaac gaaagaaaag aacctggtct ctctttactc gacttacccc aacttacttc
     6901 tgtcggagtc gtggaacctc ccgctggtga aaaaaatctt cggtccggtc tcagtactcc
     6961 cggaaaaaca actggggaaa agccgaatcg atttcctcct ccagaatggc aagcagcgta
     7021 tctgggtcga ggtcaagtcg gtgacccttg tagaaaacgg cgtggcgctc ttcccggatg
     7081 cccccacggc acgcggccgc cgccacctcg aagaattaat ccaagcgaaa aaatcaggcg
     7141 atcgggccgt cgtcttgttc gtcgcccagc gctccgacgc caaggtggtc atgcccaacg
     7201 tccgcaccga cccggccttc gcccgcacac tcgctcgcgc tctaaaagcg ggagttgaaa
     7261 tgtacgcctt tcgctgtcgg atagatcgca gaggaattgt gctggatcgc gccattccca
     7321 tccggctgcc ggaccgaaga aactttctgg cgactgcagg ggttacaaaa attacatcga
     7381 agacggagta actatggcag accaggtcga aaaatctccg gaagtaatga gccgccgctc
     7441 atttttcgcc accctgacca aactctccgg tggcttcatt gcagtgctcc tgggaatccc
     7501 aattgtgggc aatattatct cgcctttttt ccagaaaccg gaaaccgatt ggattgattt
     7561 aggcaacgct gacgacctgc acggcatggc gcccttgaaa atcccctact cggtcgatgt
     7621 gcaggacggc tggtacaaaa cgaaggccga ccgcttcgtc tacgtggtct ggcgcaatga
     7681 cgatttttta gtcctttcgc cgacctgcag ccacctgggc tgctcggtgc gctgggacgg
     7741 ccgcaaattc gcctgcccct gtcacggcgg gacatacgac agggaaggga aagttaccgg
     7801 cgggccgccg cccaaaccac tggcgcactt tgccacgaaa atcgaaagcg gccgcctgat
     7861 ggcaaaggag gtggtgtgac cctcatccga cccggcgcaa atcttctgat tggaggacga
     7921 cggagggcac tcgccccacg cgcagacact cctctatgaa aagaatcacc tcttggttag
     7981 acaagcgctt cggcctgggc tggctcatca aaaaagagtg gtacaagccc ctaccacctt
     8041 ccaccaactg gctcttcagt ctggggagcg tggcgctctt tctcttcgtc gtccagatcc
     8101 tcaccggcat tttcttagca ctctactacg tccccacacc cgatcacgcc tacgagagcg
     8161 tccagttcat caacgaaaag gtcatcttcg ggaaactgtt gcgtggcatt cattcctggg
     8221 gtgcgggact tctgatgatc atcctgccgc tccacctgtt acgggtcttc ttttacggcg
     8281 cgtataagaa gccgcgcgaa acgacctgga tggcgggcgt tctgctcctg ggtgtgactt
     8341 tcactttcgc cttcaccgga tacttattgc cctgggatga aaagggtttc tgggccaccg
     8401 tggtcggaac gaacatcgcc ggctcgccgc ccgtactggg caaatacgtc gccacctggc
     8461 tcaaaggcgg cgtcaccttc ggggctttga ccctctcgcg cttctatgcg attcacgttc
     8521 tccttctgcc ggcaaccttc ctcgtcttgg tttcggtgca cctctatctg gtgctcaagc
     8581 acaacaccgc cggccactgg gatccgcaga agaatgattt cagccgcggc ttcccctttt
     8641 acccgattca ggtggggcgc gacgccctgg cggcgctggt agtgctcgca attgtcggtg
     8701 ttctggcctt tgtcaaaccg ccccaccttt tggacgtcgc caacccgtcc aacacctcct
     8761 tcatcccccg gccggagtgg tatttcctgt tccacttcca gttgctcaag tatttcccag
     8821 gttcgtcacg tgttattgcc accgtcattc tgccgacgct cttctttctg gctttgattc
     8881 tcctcccctt ttacgaccgc cgtccgcggc gcagtccgct caagcggccg gttgctttga
     8941 ctctggcctc cctcacggtc gtgagcgtca tcaccctgac ggtgcttgcg attcaagagg
     9001 acgtacgctt atccggcttc gggcccaagg tgccgctgaa cgctgtccag aaagccggcc
     9061 tggaggtctt caacacctac ggctgtaaaa tgtgtcacgg cgagggcggc taccccggcg
     9121 gccagggacc gagtttgatc gacgctacta cccggcacga ccaaacctgg ctgatgcaac
     9181 acttcaaaga cccgcagtcg cttgttcccg gctccaagat gccgaaa
//
LOCUS       DBNGZA010000393         9714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_290256, whole genome shotgun sequence.
ACCESSION   DBNGZA010000393 DBNGZA010000000
VERSION     DBNGZA010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_290256"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16FU_08490"
     CDS             complement(<1..209)
                     /locus_tag="AC16FU_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994444.1"
                     /translation="MNQKQQTVPFKTTVLAPLDTSSAAPAEDVTVLQQAYRELEQRCR
                     SLTAELDRLKPLAALGERIALVAHEL"
     gene            complement(319..834)
                     /locus_tag="AC16FU_08495"
     CDS             complement(319..834)
                     /locus_tag="AC16FU_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2994445.1"
                     /translation="MSAPETPHQSAVPQILVIEDELYLRETLADYLGQQGYRVRTCES
                     GEQGVLLHQEERSDVALIDLWLPGRDGLEITRLLLEQDASLRIVLMTCRPNLVSATDA
                     LREQVFDYLIKPFRLPEVRDVVERAVRHAGLCIPAPPQVEPFGPPGPDEYPASGGTLR
                     QFPPLVWRFVF"
     gene            complement(958..1230)
                     /locus_tag="AC16FU_08500"
     CDS             complement(958..1230)
                     /locus_tag="AC16FU_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR2994446.1"
                     /translation="MSLEKHKVLVVDDELLIRDLLYDFFSGRECEIAVAASGQEALKQ
                     LEQHSFDVILVDLKMPDMDGLDLARAIRASGHTSPIILITGYPSVE"
     gene            1702..3654
                     /locus_tag="AC16FU_08505"
     CDS             1702..3654
                     /locus_tag="AC16FU_08505"
                     /inference="COORDINATES: protein motif:HMM:NF040218.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HIR2994447.1"
                     /translation="MRFAKFFFVLAGLTIFLSSATARADSVLVVTPPSLPPFTMAEGG
                     PNPAPQKLRIRNCCPGELRFGVFTNPSAWLKVNGQPAVINLTTPDSVDISVEATTLPP
                     GVYTGEVTVSSPLTFPVTVPCTLTVNAAPQPVIDVSPPSFFFTTQQGTNPPAQQLIVT
                     NGGTGTLIYSLFVSGTWLRADGSPTGFQNHTAPDTTTISVNTQGLDTGTYIGQLTLTA
                     SGVTPATVPVTLTVTAPPQPLIHVNPASFSFTAEQGTNPPSQNLAVTNGGAGTLVYSL
                     FVTASPSGWLLANGSPSGFLNHTAPDNVTISVNTGGLPPGLHVGQLTLTASDAPTVNV
                     PCTLTVTFNPPIIDVAPDSFIFTAMQGGPDPAPQVLSVTKGSAVNFNFNAFKTATWLL
                     VNPSVNVPAPAFLTVSAQVGSLPVGDYRDVIDITATGAQTKHVPVRFNVVQGPLLDVS
                     PTNLFFTGQVGDPDPAAQTVRIDNTGAGTLNWQAAPTQPWVSITPNSGTAPPAQNASV
                     GVHPAGLIPNIYADTVTVSGNAPNSPKQVIVFLQLNGPTAVREISESQLPRAYTLNQN
                     FPNPFNPSTRIRFALPKEGKVTLEVYSILGSKVATLVDEFLSAGTKEAVWNGTDDAGQ
                     TQASGIYFYRLKAGDFTDVKKMLLTK"
     gene            3812..5026
                     /locus_tag="AC16FU_08510"
     CDS             3812..5026
                     /locus_tag="AC16FU_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994448.1"
                     /translation="MKIKFCLLSVVAVLGLFASGCEKKVNRIQGPAPTGVLRPPSGAV
                     IPSPLLTVSFGGTSRELWPFTGSDFSGAPRDPVNLIFVGQSDPRIIRAALLSLNGDRT
                     AFGFPNVFPFNCTWTDAVGDVQTGYATASGWVGSAIQLACGTYGPLRFHLRLFNVGDW
                     TLGGAHFEVLIPGTTEHQVLSWELAEQLVVVDFLRSGLLDPTLPLLPTGVINPSPYRE
                     IPAVIYNGLPADLKALIGGPPGNVSDPVPLTTDGKATILNIAQSVNGLPVVAKQDFVV
                     QFDQVIPKPFCASGPFDFVLVQGPVNFSQKIVMTPAGNFLSRFLAQGHLDVTPVNPLT
                     DPPTPLGETYRALVNQDNRGIITDNVTLVSSFQMQIEIPPTGPFRGRLVVKLKVGPGG
                     SNQFSLSVNCLP"
     gene            complement(5111..5527)
                     /locus_tag="AC16FU_08515"
     CDS             complement(5111..5527)
                     /locus_tag="AC16FU_08515"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR2994449.1"
                     /translation="MRILFLCTGNSARSQMAEGFGRKLFPPEVEIWSAGVEPRKLNSL
                     AVQVMAERGINISRQFSKGVDAVPPPVDLVVTLCGDAAERCPSFASSPRHLHWELPDP
                     ALPQKDKAVPLALFREVRDRIESHVQSLAKELAVRR"
     gene            complement(5576..5660)
                     /locus_tag="AC16FU_08520"
     tRNA            complement(5576..5660)
                     /locus_tag="AC16FU_08520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5625..5627),aa:Leu,seq:taa)
     gene            complement(5962..7215)
                     /gene="nrfD"
                     /locus_tag="AC16FU_08525"
     CDS             complement(5962..7215)
                     /gene="nrfD"
                     /locus_tag="AC16FU_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2994450.1"
                     /translation="MEPFLQSLPGGTGGISGFVFPNEFELHWGILIVVYPYLTGLVAG
                     AFILASLERVFKVQALEPIYRLSLLTALSFMIVAPLPLLAHLGHPERALEMFLTPHTS
                     SAMAMFGFVYAWYLAVVLLLEIWFDYRRDIVNWSKQKKGPAKVIYRILTLGVSDVSEG
                     AVRFDHKIGKIITVIGIPSAFLLHGYVGFIFGSVKANPWWSSVLMPIVFLFSAIVSGI
                     ALVILVYMVTAMVRWKTINMVCLDKLAELLLYAIIVDFSLELLDFIHRLYESEESINI
                     LSQMISSRLFISLIITQITIGTIIPVISIVMVRTRTVHVEMRRILYFIAAILVQVGIF
                     STRWNVVIGGQLFSKSLRGLTVYKLDLFGPEGVLMAGALLILPFIIIYVLTKLLPPWP
                     QGEQDNISGDEAGRTDAAIVLVKAS"
     gene            complement(7218..7988)
                     /locus_tag="AC16FU_08530"
     CDS             complement(7218..7988)
                     /locus_tag="AC16FU_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2994451.1"
                     /translation="MFTVPKMPRREFLTTFAQKVSGLAGSMVALGSLTALAEEKNDKY
                     NPSEHKYAMAIDIQKCIGCGRCVEACKKENNVPLDDAHFRTWVERYVIAEDGETYVDS
                     PSGGMYGFPDLKEDMKVFRTFFVPKLCNHCENPPCVQVCPVGATYISPDGVVLVDEKY
                     CVGCRYCIQACPYGARYFHPVKKVAEKCTFCYHRITKGLLPACVEVCPTQARIFGDLK
                     KTSSPLRRFFHFMKISVLKPWMNTEPKVYYGGLDGEVK"
     gene            complement(9170..>9714)
                     /locus_tag="AC16FU_08535"
     CDS             complement(9170..>9714)
                     /locus_tag="AC16FU_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2994452.1"
                     /translation="IFDMSAYWFTYSWLPGYLHQQRQFSLAKSAAWILVTQCGGFLGY
                     FTFGFVADRLGRRPAYSIYSFLMAAGLVMITLLWETIAVYPAVILACMFLVGFGTGMF
                     GGYGPLFSELFPTEIRNTAMGSAFNLARGVQFFTPVIIAKIASLYGLGGGISLAAFFA
                     LLTGIWIWTFPETKGKKLSA"
BASE COUNT         2276 a         2763 c         2499 g         2176 t
ORIGIN      
        1 agttcgtgcg ccaccagggc gatccgctcc cctaatgccg ccaaaggttt cagccggtcc
       61 agctcggctg tgagtgaccg gcaccgttgc tccagttcgc gatatgcctg ttgcagtaca
      121 gtgacatctt ccgccggcgc agccgaagaa gtgtccagcg gtgccaggac ggttgtttta
      181 aaaggaacgg tttgctgctt ttgattcacg tgccgggcgc ctcgccacct tccctgtact
      241 tcacttttat cccgcacatc gaaaaagaaa tataaagcgt aaacgccccc tctgccaacg
      301 agaaattttg gggacgaatc aaaaaacgaa gcgccaaacc agaggcggga attgccggag
      361 cgttccgccg gaggcgggat attcgtccgg ccccggtggg ccgaacggtt caacctgcgg
      421 cggtgcgggg atgcaaagcc cggcgtggcg cacggcccgc tccaccacgt cccgcacctc
      481 cggcaggcgg aacggcttga tcaggtagtc aaagacctgt tcccgcagtg catccgttgc
      541 actgaccaga ttgggccgac aggtcatcaa tacgatgcgt aaagacgcat cctgctccag
      601 gaggaggcga gtgatctcca gaccgtcccg tcccggcagc cagagatcga tgagcgccac
      661 gtcggatcgt tcttcctggt gcaacaacac gccctgctct ccggactcgc aggtgcggac
      721 ccggtacccc tgctggccca ggtagtcagc cagtgtctcc cgcaggtaga gttcatcttc
      781 aatgacgaga atctgcggaa ccgccgactg gtgcggagtc tccggagcgg acatttaaac
      841 tttttccccc tgctcgcggt actcccgggc cgcccggctc accaggtgga acagctgatt
      901 gatgttgaac ggtttcgtta catagtcgta cagccgaatc cgcagtgctt ccaccgccta
      961 ttccacgctg ggatagcccg tgatgaggat gatcgggctc gtgtgcccgg aagctctgat
     1021 tgcccgggcc aggtccaacc catccatgtc cggcattttc agatcgacca gaatgacatc
     1081 gaaagaatgc tgctccaact gcttcagcgc ctcctgtccc gaagcggcga ccgctatctc
     1141 acattcgcgg ccggagaaga aatcgtataa caaatcgcgg attaaaagtt catcatcgac
     1201 caccagcact ttgtgcttct caagcgacac cgggatcctc gtgttggttc gtttagccgg
     1261 tatacctctt ttatccttat cggacagtta cggctgcaga aatagcccaa atcgacgcca
     1321 aaccaccccc aaccgtccgg tactaactaa ctctctctca gagaattacg gccaatctcg
     1381 actgcaatca ccggcgcttt ttatgcaacg gtttaccgat tcttttgcga cggccaaaaa
     1441 gattccaggg atatcttcct gaaatgcagg aaagacgcgc tttgcaaccc ggcccgaacc
     1501 gcgggaacga accaatattg acggcacagg atatgctcta ttatcgcttg acagtatgcc
     1561 ggttagtcct atatttcggg tgcaacctga gggaggctgc ccgcaaggag taaccttggc
     1621 gtttgaggag tctttgtctt ttggtgtcgg ttcgtgcgcg agcacggcag aggaagtgag
     1681 tttgctttga ggaggatcgg gatgcgtttc gcaaaattct ttttcgtact ggcgggcttg
     1741 acgatttttc tctcgagtgc caccgcgcgg gccgattcgg tgctggtggt cacgccgcca
     1801 agtctccccc cgtttacaat ggccgagggt gggccgaatc ctgcgccgca gaaactgcgc
     1861 atcagaaact gctgtcccgg ggaactgaga tttggagttt ttacaaatcc tagtgcctgg
     1921 ctaaaagtga acggccagcc ggcagtaatt aatcttacca ctcccgactc cgtggatatt
     1981 tcggtcgagg ctactactct gcctccggga gtgtacacag gagaagttac ggtttcgtcc
     2041 cccctcacat ttcccgttac ggtgccctgc accttaaccg ttaacgccgc tccccagccg
     2101 gtcatcgatg tcagtccgcc cagtttcttt ttcaccaccc agcagggcac caatcctccg
     2161 gctcagcagt tgatcgtgac gaacggcggg acgggaacct tgatctacag cctctttgtc
     2221 tccggcacct ggctccgggc cgatggttct cctaccggct ttcaaaatca caccgccccg
     2281 gatactacaa ccatttccgt caacacccag ggtctggaca ccggtacgta catcggccag
     2341 ctgaccctca ccgccagcgg agttacgccc gctacggttc cggtcacgct gacggtcact
     2401 gcgcctcccc agcccctgat tcacgtcaac ccggccagtt tttccttcac tgccgagcag
     2461 gggaccaacc ctccctccca gaacttagcg gtgacaaatg gtggcgcggg aacgctggta
     2521 tacagcctct tcgtgacggc cagtccctca ggctggctcc tcgccaatgg ctccccttct
     2581 ggttttctga atcataccgc cccggataac gtgacgatat cggtcaacac cgggggactt
     2641 ccccccggcc tgcacgtcgg ccaattgact cttaccgcca gtgatgcacc aacagttaat
     2701 gttccctgca cgctcacagt taccttcaac ccgccgataa tcgacgtcgc ccccgattcg
     2761 ttcatcttca ccgccatgca gggcggaccc gatcccgccc cgcaagtgtt aagtgtgacc
     2821 aagggcagtg cggtgaattt caacttcaac gctttcaaga ccgccacctg gcttttggtg
     2881 aatcccagcg tgaacgtgcc ggcgcccgct tttctcaccg tctcggctca ggtcggctca
     2941 ctgccggtgg gtgactaccg tgacgtcatt gacataaccg ccaccggcgc acaaaccaaa
     3001 cacgtgccgg tgcgcttcaa tgtcgttcag ggaccgcttc tggacgtctc gcccaccaat
     3061 ctgtttttca ccggacaggt cggcgacccc gatcccgcgg cgcaaaccgt ccggatcgat
     3121 aatacgggcg ctggaacttt gaactggcaa gccgctccta ctcagccctg ggtttcgatc
     3181 acgccgaatt ccggcacggc cccgccggct cagaacgcca gcgtgggggt ccacccagcg
     3241 ggcctgattc ccaacatcta cgccgacacg gtcacggtga gcggcaatgc gccgaactct
     3301 cccaagcagg tcatcgtctt cctgcaactg aacgggccta ccgccgtgcg cgaaatatcc
     3361 gaaagccagc tcccccgggc ttacacgctc aaccagaatt tccccaaccc cttcaacccg
     3421 tcgacgcgga ttcgctttgc cctccccaag gaaggcaaag ttacgctgga agtctatagc
     3481 atcctgggct caaaagtcgc caccctggta gatgaattcc tctccgcggg gacgaaagaa
     3541 gcggtctgga acggtactga cgacgcggga cagacgcagg cctccggcat ttacttctac
     3601 cggctcaagg cgggcgattt caccgacgtg aagaagatgc ttttgacgaa gtgacggttg
     3661 cggaaaatgt aactactgcc gccacggaat aggcggcgca aactttggct gtcttctcca
     3721 gggaggtggt gccttacaag agtaaaccga gaaccggaat atcctgcgag tgtttttgac
     3781 ccttctcttt taagtgaaag ggggaatatc cttgaaaatt aaattctgcc tcctcagcgt
     3841 cgtggccgtt ttaggacttt ttgcttctgg ctgcgaaaag aaagtaaatc gtatccaggg
     3901 accggctccc actggtgtgc tgcgcccgcc ttcgggagca gtcattccgt ctccgctctt
     3961 gacggtttct tttggaggaa cgtcccggga attgtggccc tttacgggaa gtgatttctc
     4021 cggtgcgccc cgggatccgg tcaatctcat tttcgtcggc caatcggatc ccaggatcat
     4081 ccgggcggcc ctcttatccc tgaatggcga ccggaccgcc ttcggctttc cgaatgtctt
     4141 tccgttcaac tgtacctgga ccgatgcggt gggagacgtt cagaccggct atgccactgc
     4201 ctccggttgg gtgggcagcg ccattcaact ggcctgcggt acctatggtc cgctccggtt
     4261 tcacctgagg ttgtttaatg tcggcgattg gactttgggc ggcgcccatt tcgaagtgct
     4321 cattccgggc acgacggaac accaggtgct gagttgggaa ctggcggagc aattggtcgt
     4381 cgttgatttc ctgcgcagcg gtcttctgga tcctaccctt cccctcctgc cgaccggagt
     4441 aatcaaccct tccccgtacc gcgaaattcc agcggtaatc tacaacgggc tgccggctga
     4501 cctgaaagcg ctcatcggag ggccgcccgg taatgtgagc gacccggtgc ccctcaccac
     4561 tgacgggaaa gccaccattc tcaatatcgc tcaaagcgtg aatggcttac ccgtcgtcgc
     4621 caagcaggac tttgtggttc agtttgacca ggtgatcccc aagccgttct gcgcctccgg
     4681 tccctttgat tttgtgctgg tgcaagggcc ggtgaatttc agccagaaaa ttgtaatgac
     4741 ccccgctgga aatttcctga gccggtttct tgcccagggt catttggacg tgacgccggt
     4801 aaatccgctc accgacccgc caacaccgct cggtgagacg tatcgggcgt tagtcaacca
     4861 ggacaacaga ggaataataa ccgacaacgt caccctggtc tccagtttcc agatgcagat
     4921 tgagattcct ccgaccggcc ccttccgcgg ccgactggtg gtcaagctaa aggttggtcc
     4981 cggcggctcc aaccagtttt ccctgtctgt taactgcctg ccgtagggat tagcgcagaa
     5041 cggaaaaccc acgcacccgc gtgggttttt tatttctggg cgaagttttt ctgccaatcg
     5101 agcgccataa ttaccgtctg acggccaatt ctttagccag cgattggacg tgggactcga
     5161 tcctgtcgcg cacttcgcga aaaagtgcga gcgggactgc tttgtctttc tgcggcaggg
     5221 ccggatcggg cagctcccag tgcaggtgtc gtggggaact tgcaaaagat ggacagcgct
     5281 ctgccgcatc tccgcacaga gtaaccacta aatccactgg cggcggaaca gcatcaactc
     5341 ctttcgagaa ctgccgggaa atgttgattc ccctctcggc catcacctgc actgccagcg
     5401 aattgagttt tcgaggttcc accccggcgc tccagatttc aacttccggc gggaagagct
     5461 ttcggccaaa accttcggcc atctggctgc gggccgaatt gccggtgcag aggaataaaa
     5521 ttctcatgcg aaactatctt cgcattctca ggggaacatc ggtcaaagcg aaaaatgccg
     5581 gaggccggac tcgaaccggc accccgaagt atcggggagg gattttaagt cccttgcgtc
     5641 taccattccg ccactccggc attttcttct caggcaatac cttacttcac ttttccaaga
     5701 tagtaaatta ttgggcttgt gaccaaattg agtccctatg ctgccacctt tcgatggcga
     5761 aagaatactt catcggaaaa ggattcgaga aaaagaaacc tcttccgccg aatgaacgtt
     5821 ttgcgaaccg caaactgaag ccctttgaaa aagtgtcata gaattggggg taggtacaca
     5881 cccgaaattc ttgcaccgag agtagtcgag gggtgggcac ccggccgaag actccggata
     5941 tttcaacgca gagatttcag gtcaggatgc tttcacgagg acgatggcag catctgttct
     6001 gccggcttca tcaccggaaa tattatcttg ttcgccttgg ggccagggcg gcaacaattt
     6061 tgtcaagaca tatatgatta taaacggcaa aatcaacaaa gcgccggcca tcaaaacccc
     6121 ttcggggccg aacaagtcca gcttataaac ggtcagcccg cgcaaactct tcgagaaaag
     6181 ctgcccgcca attaccacat tccaacgagt gctgaaaatg ccgacttgta ccagaatggc
     6241 ggcaatgaaa tacaaaatcc gccgcatttc gacatgaacc gttctcgtcc ggaccatgac
     6301 gatcgatatc acgggaatta tggttccgat agtaatctgg gtaataatca gactgatgaa
     6361 caatcggctg gaaatcattt gcgaaagaat attgattgat tcttctgatt catagagtcg
     6421 atggatgaag tccaggagct ccagtgaaaa atccactata atggcataga gcaaaagttc
     6481 cgcaagttta tccagacaga ccatgttgat tgttttccag cgaaccattg ctgtcaccat
     6541 ataaaccaga ataaccaaag caattccgga aactatagcg gaaaagagaa acacgatggg
     6601 catcaggacg ctgctccacc aggggttggc tttgacggac ccgaagatga agccaacata
     6661 gccatgcagc agaaatgccg aaggaatacc tatgaccgta ataatctttc caattttgtg
     6721 gtcaaacctg accgctcctt cagatacatc gctgactcca agtgttaaaa tgcgataaat
     6781 aaccttggcc ggcccttttt tttgtttgga ccaattaaca atgtcgcggc gatagtcgaa
     6841 ccatatctcc aaaagtaata ccactgccaa ataccaggca tagacaaaac cgaacatggc
     6901 catggccgac gaagtgtgcg gggttaaaaa catttccagc gcgcgctccg ggtgccccag
     6961 gtgagccaaa agcggcagcg gcgctacaat cataaaagaa agggccgtca ataaggaaag
     7021 ccgataaatt ggctcaaggg cctggacttt gaagacgcgc tccagcgagg ccagtataaa
     7081 ggcaccggcc accagtccgg tcaggtaagg atagactaca atcaagatcc cccagtgcaa
     7141 ttcaaattca ttagggaaca caaagcccga gatacctccg gttccacccg gcagtgattg
     7201 cagaaaaggt tccatgatta tttcacttct ccgtcgaggc cgccataata cactttgggt
     7261 tcggtattca tccacggttt taagaccgag atcttcataa aatgaaagaa tcttctaagc
     7321 gggctgctgg tttttttcag atcgccgaaa atgcgggctt gagtcgggca aacttccaca
     7381 caggcgggca aaagcccttt ggtgatgcga tgatagcaaa atgtgcactt ctcggctacc
     7441 ttctttaccg ggtggaaata acgagctcca taaggacagg cctggatgca gtaacgacag
     7501 cccacgcagt atttttcatc gaccagtacc accccgtcgg ggctgatata ggtggcgccc
     7561 accggacaaa cctgaacgca aggcggattc tcacaatggt tgcaaagctt gggtacaaaa
     7621 aacgtgcgaa aaaccttcat atcttctttc aggtccggaa agccatacat gccaccgctc
     7681 ggactgtcca cataggtttc cccgtcttcg gctatgacat accgctctac ccaggtacgg
     7741 aagtgcgcgt catcgagagg aacattgttt tctttcttgc aggcctcaac acagcggccg
     7801 cagccaatgc acttctgaat gtcgatggcc atcgcatact tatgttctga tggattgtat
     7861 ttatcgtttt tctcttccgc cagggccgtc aaactgccca gcgccaccat tgacccggcc
     7921 agtccggaaa ctttctgggc aaaggtcgta agaaattctc gccttggcat tttgggcaca
     7981 gtaaacatta ctacttcccc tccgggtcat ggggataatg acaatcccag cacacgtgcc
     8041 gttcgctgtg ggactctata tcgactctgg gaatctcgct tgagccggtc gcattcttag
     8101 catggcagtt ggcacaggtc tgaatattgg ttggcttctc ggcatggatg aggcgggggt
     8161 tgacattatg acctttgcgc acattgtggc actgcgtaca gggcagattg gtgtgtactg
     8221 atacaagctt ttggttggca atactcctgt ggcaggcact gcattcctcg ggcgtgtgag
     8281 gaggcctggg atcatgggga ttatgacagg acatacaggc tttcccagga ttgtgctgat
     8341 tctttagaat ttgtggaaaa ccggtcggac gggaaggatt atatccatgg caaacgggac
     8401 agaattctcg ccctttgggc ttgaaaggca gattttcatc gggagcgtta gcgtggtcag
     8461 cacacggccc atgacaggcc tcgcacggta ctttacgatg aaatgaaccg gctttaagtt
     8521 cttttatttc agaatggcag tccgcacaga cctctgctcc ggcatatttg attttgatct
     8581 cagaagcctc ctttagcgcg tcgcctctat aaaagcccat cttgccgaaa gatggcggga
     8641 cgaacatttg acgggcggta atgaaagccg cgatgaccag cgcgaacaga acgaaaagtg
     8701 ggattaactg tctcggaatt ctggacataa ccttcgccta aacacccttg ggtaaaaacc
     8761 tacgttttct actttacgat acaatactat tattataata tatcggctga aaaatgaaac
     8821 accagccccg ccttgtcaag tgggtaggtt gagtggagtt gctgaatagc tcagccactt
     8881 tccaaaacca tccctttatc tcgacccaat acggtgcggc agattcgaga gtaaagcagt
     8941 ttgccccaac tcgctgtcta ttaacggctt atgccacttc cccggttcaa ctcgttgggt
     9001 tttgttcgga agaagttctg cctatgtaga agtgaacgtc cccgagctta ttgatacgcg
     9061 caccattcgg ttcgaggttt gttcatagga ggtgcccggg tggggcaata tgcgaaccgt
     9121 tacccgcaat tctttgaaaa agagccttat ctttgggatt cttatatcgt taagctgaaa
     9181 gcttttttcc cttcgtctcc ggaaaggtcc aaatccagat accggtcaaa agggcaaaaa
     9241 aggcagccag agaaattccg cctcccagcc cataaagaga ggcgattttg gcaataatca
     9301 cgggcgtgaa aaactgcact ccacgggcca ggttgaaagc cgagcccatg gcggtattgc
     9361 ggatttcagt cgggaaaagc tccgaaaaaa gcgggccata gccgccgaac atcccggtgc
     9421 cgaagccgac caagaacata caagccaaaa tcaccgccgg atagacagcg attgtttccc
     9481 acagaagggt aatcatcacc agtccggccg ccataaggaa agagtaaatc gagtaagccg
     9541 ggcggcggcc caagcggtcg gccacaaagc cgaacgtaaa ataccccaga aatccgccgc
     9601 attgggtcac caaaatccaa gcagctgact tggccaagga aaattgcctt tgctggtgca
     9661 agtaacccgg caaccaggag tacgtgaacc aataagcaga catatcaaaa atcg
//
LOCUS       DBNGZA010000394         6789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2197764, whole genome shotgun sequence.
ACCESSION   DBNGZA010000394 DBNGZA010000000
VERSION     DBNGZA010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2197764"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="AC16FU_08540"
     CDS             <1..1092
                     /locus_tag="AC16FU_08540"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR2994453.1"
                     /translation="NLELGVTAGKELQVTVPTFRPDVTREIDLIEEVARIWGYEKIPR
                     SHSARGDAYVPTHAEDELTSAIKNFLCAQGYNEVVTNTLVDPGWLEKLGLPASPVRLQ
                     NPIAEALSVLRTGLVSSLLEVVRRNRNRQADLIKLFEVGRIFLSSREKLPREKLVLGI
                     VATGIRQRQSWDAAEAPVDLFDLKGVLDSLAQRFRLNGFALEPKAVPFLDPESSFRLL
                     LNGAEVGSLGAVGSEAQEFFDLSDAVYYAECDFGALLAAGVSRPTYEPLDRYPFVERD
                     IAVVVDRTCLAGDLLAAIRQTGGELVREARLFDLYQGQPLPLDKRNLAFRLRYQSRER
                     TLTDREVDAIQERIVAALKKQFDAALRTY"
     gene            1137..1433
                     /locus_tag="AC16FU_08545"
     CDS             1137..1433
                     /locus_tag="AC16FU_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994454.1"
                     /translation="MAQDVLQKLQDRVNQTLETVEKLKQENQTLSAGRHALTEQLALA
                     QKNLTALTVQYQKMQKEKERQDQDYQKRKVALRQKLETVLTKVATVEERLRNFT"
     gene            1530..1847
                     /locus_tag="AC16FU_08550"
     CDS             1530..1847
                     /locus_tag="AC16FU_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR2994455.1"
                     /translation="MTSASTPTDQTTLRVNIFGEEYPVRAKADPDYITAVAKYVDQKM
                     RDIAQKIPNRSFANVAVLAALNITDELFRERQDKEKKLTDVEEKTQEILVWLDQKLAE
                     GQI"
     gene            1945..3510
                     /gene="rny"
                     /locus_tag="AC16FU_08555"
     CDS             1945..3510
                     /gene="rny"
                     /locus_tag="AC16FU_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR2994456.1"
                     /translation="MTNFILILIIAAAAVAAGIALGFWLSRRIGAEKLARAGELAQRM
                     IREAEKEAEIKKKEALLEAKDEWYRAKQNFERELQNKKLEFQKTEKRFQEREAEVARK
                     VDFITKKERELQNREKVLLAREKGITTREGELERLVVEQNRQLERVAQMTTEEAKKQL
                     MENLINQARAEAAQTIKQMKDEAEQTGEREARNIITLAIQRCAADHTVETTVSVVNLP
                     SDEMKGRIIGREGRNIRAFEAATGCDVIVDDTPEAVVLSGFDPVRREIARMALEKLIL
                     DGRIHPARIEEVVTKTQKEMETIIREAGEQACFELGIHNLHSEVVKTLGRLKFRTSYG
                     QNVLAHSQEVAMLCGLIAAQLGLDPQIAKRAGLLHDIGKAIDRETEGTHTQIGLNFMS
                     KYNEDPAVLNAIAAHHEDVIQETPYAVLVQAADAISGSRPGARRESLEGYVRRLEKLE
                     EVADSFKGVAKAYAIQAGREVRVMVENDQVDDSGAAELAGQIAKKIQAEMEYPGQIKV
                     TVIREMRAVDYAR"
     gene            3590..4228
                     /locus_tag="AC16FU_08560"
     CDS             3590..4228
                     /locus_tag="AC16FU_08560"
                     /inference="COORDINATES: protein motif:HMM:NF018626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HIR2994457.1"
                     /translation="MKISFTGFWFLGLFGTLVVLERVNLPKRESSFGQRLLTAAREYL
                     QEKPTYDLSRRYRKIGFPKGDIRRNKGVCADLVIRVYREVGIDLQERMHEDMKKNFHI
                     YLARRNYGASQPDPNIDHRRVPNLVTFLSRHGLTLTPSLSPERLACWQPGDIIITDYR
                     REGYETHIGFVSDKRNSQGVPYIIHHRPVYPVEEDSLAARPILGHYRYPKTE"
     gene            complement(4283..5701)
                     /locus_tag="AC16FU_08565"
     CDS             complement(4283..5701)
                     /locus_tag="AC16FU_08565"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR2994458.1"
                     /translation="MNGPPLPTVFHLTYLLAAILFIVGLKFLSHPARARYGNLIAALG
                     VTIAGLTTLASLWVPGSGYHNQGLILAGIAVGATAGTISARRVAITNMPQMVALLNGF
                     GGGAAALISTVEFLHTAGSGLRPTATAAASTMFGCVIGSISFSGSLVAFGKLQGIISE
                     RAITSTFQKILNAFLFIGLLAVIVTIALGGGAPWFLTLLGGALAFGVLMVIPIGGADM
                     PVVISLLNSFTGLAVAATGFVLANNALIISGTLVGASGTLLTMLMCKAMNRSLANVLF
                     AGVGAGPVAAPAAGRGAPQTVHTITPEDAGVLLANIQSLIIIPGYGMAVAQAQHVVRE
                     LADMLARQGVEVKYAVHPVAGRMPGHMNVLLAEANVPYDQLYDLDQINPDFERCDVAL
                     VIGANDVVNPAARTDKSSPIYGMPILDADKAKHTIVIKRSLNPGFAGIDNALYYAPKT
                     YMLFGDAKGAVAKLVEALKAAA"
     gene            complement(5698..5991)
                     /locus_tag="AC16FU_08570"
     CDS             complement(5698..5991)
                     /locus_tag="AC16FU_08570"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR2994459.1"
                     /translation="MVSLAEVYVFVLAVFVGFEVISKVPVVLHTPLMSGTNAIHGIVM
                     LGGVLVLASADTPLLTVLGFVAVVFGAMNLFGGFVVTDRMLEMFKKKPGSKRP"
     gene            complement(5994..>6789)
                     /locus_tag="AC16FU_08575"
     CDS             complement(5994..>6789)
                     /locus_tag="AC16FU_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR2994460.1"
                     /translation="SSMSTVAGYKAALLGAASLGKFFPLLMTAAGTIAPARVFVLGAG
                     VAGLQAIATSRRLGAVVAAFDVRPAVREEVQSLGAAFVQLEMSQESLVGAGGYAKELS
                     ADHVRRERELIHQHVKNSDAVISTAMVPGKKAPVLITEAMVKDMRRGAVIIDLAAEMG
                     GNCELTLPSQMVERCGVKIHGPVNLAATMPEHASQMYARNVSALLNHLVKKGELVLDF
                     SDEITRGCCLTHGGRRLLDAAGQPLTPPGGQSAAQPASEPATRVKA"
BASE COUNT         1514 a         1955 c         2092 g         1228 t
ORIGIN      
        1 aacttagagt tgggcgtcac cgccggaaaa gagctgcagg tcaccgtgcc gactttccgt
       61 ccggatgtca cccgggagat cgatctgatt gaagaagtcg ctcgtatctg gggttatgag
      121 aaaatccccc ggagccattc ggccaggggt gacgcatacg taccgactca cgccgaagat
      181 gagctgacgt cggctatcaa aaactttctc tgcgcccagg gctacaacga agtggtgacc
      241 aatacgctgg tcgatccggg ctggctggaa aaattggggc tgccggcctc gcccgtgcgg
      301 ctgcaaaacc ccattgccga ggcgctgtcg gtcctgcgta ccggattggt cagcagtctc
      361 ctggaggtgg tgcggcgcaa ccgtaaccgc caggccgatc tgatcaagct cttcgaagta
      421 gggagaattt ttttgtccag ccgggagaaa ctgccccggg aaaaactggt gctgggaatt
      481 gtcgctaccg gtatccggca gcgccagagc tgggatgcgg ccgaggctcc ggttgatctg
      541 ttcgatctca aaggtgttct ggattctctg gcgcagcgct tccgattgaa cgggtttgcg
      601 ctggagccca aagcagttcc cttcctcgat cccgaaagtt cttttcgcct acttttgaac
      661 ggcgcagaag tcggttcgct cggagccgtc ggatctgaag cgcaagagtt ttttgacctg
      721 agcgacgcgg tctactatgc cgaatgtgac tttggcgccc ttttggccgc gggtgtttcc
      781 cggcccacct acgagccatt ggatcgttac ccgttcgtcg aacgggacat cgccgtggtg
      841 gttgaccgaa cctgcctggc cggagacctc ttggcagcta ttcgccagac cggcggagag
      901 ctggtacggg aggcccggct gtttgacctt tatcagggtc agccgctgcc gctcgacaaa
      961 aggaatctgg ccttccggct gcgctatcaa tctcgggaaa ggactttgac cgaccgcgag
     1021 gttgacgcta ttcaagaaag aatcgtcgcc gcattgaaaa aacaatttga cgccgcgctg
     1081 cggacctatt aaattgtact ttaggaaatg cgggtaagtt attgtagaat aaaaagatgg
     1141 cgcaggacgt attacaaaaa cttcaggacc gggttaatca aaccttagaa acggtcgaaa
     1201 aactaaaaca ggagaaccag acactttccg ccggccgaca cgcgctgacg gaacagttgg
     1261 ctctagccca aaaaaatctg acggctttga ccgtccagta tcaaaaaatg caaaaggaaa
     1321 aggagcgaca ggaccaggac taccagaaga ggaaagtggc cctgcggcag aagctggaaa
     1381 ctgttttgac caaagtggcc accgtggaag agcgcctgcg aaatttcact tgacagccgg
     1441 cgccggtgtg attttcttac acaccgtact tttggagagg gaagacagtt acccaaggaa
     1501 agttttttga gagaacggca gacctatctg tgaccagtgc cagcaccccg accgaccaga
     1561 ccaccctccg ggtgaacata ttcggtgaag agtacccggt gcgggccaag gcggatccgg
     1621 attacattac cgcagtcgcc aaatacgtgg atcagaaaat gcgggacatc gcccagaaaa
     1681 tcccgaaccg gtcattcgcc aacgtggcgg tgctggccgc cctgaacata accgacgagt
     1741 tattccgcga acgccaggac aaagagaaga aactgactga cgtcgaagag aaaacccagg
     1801 aaattttggt ctggttggac cagaaactcg ccgaaggcca gatttagcgt tccgtctttc
     1861 gctttccttc ttgcttccct caataaacaa ctgagttgct ctccattggg gtaggacgct
     1921 tgtgccccgg cggaaggagg gaagatgacg aatttcattc tcatcctcat tatcgctgcg
     1981 gccgcagtcg ccgcaggcat cgccttgggc ttttggctct cccgtcgcat cggcgcggag
     2041 aaactggccc gggccgggga actggcccag cggatgatcc gggaggccga gaaggaagcc
     2101 gagatcaaaa agaaagaggc gctcctggag gccaaagacg agtggtaccg cgccaagcag
     2161 aatttcgagc gggagctgca gaacaaaaag ctggaattcc agaaaaccga aaaacgattt
     2221 caggaacggg aagccgaagt cgcccgcaaa gtcgatttca tcaccaaaaa agaacgggag
     2281 ctccagaaca gagaaaaagt gcttttggcc cgggagaagg ggatcaccac ccgcgaagga
     2341 gaactggagc ggctggtggt cgaacagaac cgccagctgg aacgggtggc gcagatgacc
     2401 accgaagagg ccaaaaagca gcttatggaa aacttgatca accaggcccg ggccgaggcg
     2461 gcacaaacca tcaagcagat gaaagatgag gccgagcaga ccggcgagcg cgaagcacgc
     2521 aacatcatca ccctggcaat ccagcgctgc gccgccgacc ataccgtcga aaccacggtt
     2581 tcagtggtca atctcccctc tgacgaaatg aagggccgca tcatcggccg cgaggggcgg
     2641 aacatccgcg ccttcgaagc cgccaccggc tgcgacgtga tcgtcgacga tacgccggaa
     2701 gcggtggtgc tttccggttt tgacccggtg cggcgcgaga tcgcccgcat ggcgctggaa
     2761 aaattaattt tggacggacg catccacccc gcgcgcatcg aggaagtggt gaccaaaacc
     2821 cagaaggaaa tggagaccat catccgcgaa gcgggagagc aggcctgttt tgagctggga
     2881 attcacaacc tgcactcaga agtggtcaag accctgggaa ggttgaagtt ccgtaccagt
     2941 tacggccaga acgtcctggc ccacagccag gaggtggcca tgctctgcgg cctgatagca
     3001 gcccagctgg gtctcgatcc gcaaattgcc aaacgcgccg gccttctgca cgacatcggc
     3061 aaagcgatcg accgcgaaac cgaaggcacc cacacccaga tcggcttgaa cttcatgagc
     3121 aagtacaacg aggatccggc ggtcttgaac gccattgcgg cccaccacga ggacgtgatc
     3181 caggagacgc cgtatgcggt tctggtgcag gcggcggatg ccatttccgg ttcgcggccc
     3241 ggcgcccggc gggagtctct ggaaggctac gtccgccgtc tggaaaaatt ggaggaggtg
     3301 gccgactcct tcaaaggcgt ggccaaagcc tacgccatcc aggcggggcg ggaagtgcgg
     3361 gtgatggtgg aaaacgacca ggtggacgac tccggcgcag cggaattggc cggccagatc
     3421 gccaagaaaa tacaggccga aatggaatac cccgggcaga tcaaagtcac cgtcatccgc
     3481 gagatgcggg ccgtcgatta cgcccggtaa cagtacgtat tcacccggcc gctgacggac
     3541 acaagtacct ctcgaggcaa aggcgttccc ggagggaggg gtttcctcag tgaagatttc
     3601 gttcacagga ttctggtttt tgggattatt cggtaccctc gttgtgctgg agcgtgtcaa
     3661 ccttccaaag cgggaatcca gcttcggtca gagattgctt acagctgccc gggaatatct
     3721 gcaagagaag ccgacctacg acttgagccg gaggtatagg aaaatcggct tcccgaaggg
     3781 cgacatccgc cggaataaag gtgtctgcgc cgatctcgtc attcgggttt accgggaggt
     3841 aggaatcgat ttacaggagc ggatgcacga ggatatgaaa aagaattttc atatttacct
     3901 tgcgcggcgt aactatggag caagccagcc cgacccgaac atcgaccacc gccgggtgcc
     3961 gaacctggtc acttttctct ctcgtcacgg tctgacctta actccttctc tctccccgga
     4021 gcgtctcgcc tgctggcagc cgggggacat catcatcacc gattaccgcc gcgaggggta
     4081 tgagacccac atcggtttcg tctccgacaa acgaaattct cagggagttc cctacatcat
     4141 tcaccaccgt ccggtctatc cggtggagga ggactctctg gcggcccggc ccattttagg
     4201 acattatcgc tacccaaaaa ctgagtagca tccccaaagg gttggagcga aaatttattg
     4261 atttcttggg taaatgtgcc gtttacgctg cggctttaag ggcttcgacc agtttggcca
     4321 ccgcgccctt ggcgtcgccg aaaagcatgt aggtcttggg ggcgtagtag agcgcgttgt
     4381 cgatcccggc gaagccgggg ttcaagctgc gcttgatgac gatggtgtgc ttcgccttgt
     4441 cggcgtcgag aatgggcatg ccgtagatcg ggctcgactt gtcggtgcgc gccgccggat
     4501 tcaccacgtc attggccccg atgactaagg ccacgtcgca gcgctcgaag tcggggttga
     4561 tctggtctaa gtcgtagagc tggtcgtagg gcacgttggc ctcagccaaa agtacattca
     4621 tgtggccggg catgcgcccg gccaccgggt ggacggcgta ctttacctcc acgccctggc
     4681 gagccagcat atcggccagc tcgcgcacca cgtgctgggc ctgcgcaacc gccatcccgt
     4741 accctggaat gatgatgagc gactggatgt ttgcgagcaa aacgccggca tcctccggcg
     4801 tgatggtgtg cacggtctgc ggcgctcctc tcccggcagc aggggcagcc accggaccgg
     4861 caccgacgcc cgcgaagagg acgttcgcaa gcgagcggtt catcgccttg cacatgagca
     4921 tggtgaggag cgtgcccgac gcgcccacga gtgtcccgct gatgatgagg gcattattcg
     4981 ccagcacgaa tcccgtggcg gccaccgcca ggccggtgaa cgagttcaaa agcgagatga
     5041 ccaccggcat gtcggccccg ccgatcggaa tgaccatcag tacaccgaag gccagtgctc
     5101 ctccgaggag ggtcaaaaac cacggcgcgc cgccgcccag cgcgatggtg acgatcacgg
     5161 ccaaaaggcc gatgaagagg aacgcgttga gaatcttctg aaacgtcgag gtgatggcgc
     5221 gctcggagat gatcccctgg agtttgccga aggcgacgag gctgccggaa aaggagatcg
     5281 agccgatgac gcagccgaac atggtcgaag ccgcggcggt cgccgtcggg cgcaggccgc
     5341 ttccggcggt gtgcaggaat tcgacggtcg agatgagcgc cgccgctccg ccgccgaagc
     5401 cgttcaggag cgccaccatc tggggcatgt tggtaatcgc cacccggcgc gccgagatgg
     5461 tgccggctgt ggcgccgacg gcgatgccgg cgagaatcaa accctggttg tggtagcctg
     5521 agcctggaac ccagaggctc gccaaggttg tcagtccggc gatggtcact cctaacgcgg
     5581 cgatgagatt gccgtagcgg gcgcgcgccg gatggctcaa aaatttgagg ccgacgatga
     5641 atagaatggc ggccagaagg taggtcagat gaaacacggt cggcagcgga gggccgttca
     5701 tgggcgcttg ctccccggct tcttcttgaa catctcgagc atccggtcgg tcaccacgaa
     5761 gccgccgaaa aggttcatgg cgccgaacac caccgccacg aacccgagta ccgtgagcag
     5821 gggtgtatcg gctgacgcca gcaccaggac tccgcccaac atcacgatcc cgtgaatcgc
     5881 atttgtgccg gacataagcg gggtgtggag caccaccggg actttcgaaa tgacctcaaa
     5941 gccgacgaaa acggcgagca cgaagacgta cacttcggcc agggagacca tggttacgcc
     6001 ttcacccggg ttgccggttc ggaggcaggt tgcgccgcac tctgaccgcc gggcggagta
     6061 agtggctggc cggccgcatc gagcagccgc cggccgccgt gggtgaggca gcagccgcgc
     6121 gtaatttcgt cgctaaagtc cagaacgagt tcccctttct ttaccaggtg attcaaaagc
     6181 gcactgacgt tgcgcgcgta catctggctg gcgtgctcgg gcattgtggc ggcgagattg
     6241 accgggccgt ggatcttcac tccgcatcgc tcgaccatct gggagggaag ggtgagttcg
     6301 cagttcccgc ccatctcggc ggccaggtcg atgatcacgg cgccacggcg catatccttc
     6361 accatcgctt cggtgatgag caccggcgcc ttcttccccg gcaccatggc ggtggagatg
     6421 acggcgtcgg agttcttcac gtgctggtgg ataagttcgc gttcccgcct tacatggtcg
     6481 gcggagagct ccttcgcgta gccgccggca cccaccaaac tttcctggga catctcgagt
     6541 tggacaaagg cggcgcccag actctgcact tcttcgcgta ccgcgggccg cacgtcgaac
     6601 gccgcaacca ccgcgccgag ccgccgtgaa gttgcaatgg cctggagccc cgccacgccc
     6661 gcgccgagga cgaaaacgcg cgccggcgca atggtgccgg cggcggtcat aaggagcggg
     6721 aagaacttgc cgagggaagc cgcccccaat agggccgcct tgtagcccgc gacggtgctc
     6781 atcgaggag
//
LOCUS       DBNGZA010000395         1815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1243654, whole genome shotgun sequence.
ACCESSION   DBNGZA010000395 DBNGZA010000000
VERSION     DBNGZA010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1243654"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="AC16FU_08580"
     CDS             complement(<1..883)
                     /locus_tag="AC16FU_08580"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR2994461.1"
                     /translation="MDKFVITGRQPLSGSVKIEGSKNAVLPILAAALLVEKGTTVIHN
                     VPDLVDIDTFLKVIAQLGAKVNHDRQAKTVSIEASTLTKYEAPYDLVRKMRASFLVLG
                     PLIARLGKARVSLPGGCVIGPRPVNLHVYGFQRLGAQLSEDRGYIVAEVPPATGQLVG
                     TTICFDRPTHTGTENLMIAACLAKGKTTLVNAACDPEVVDVANYLNATGGKVEGAGTS
                     TVTITGVRRLHPVEYTPMPDRLESATFLMAAAVTGGYVEIDNGKPEHLDMVVSKLTEM
                     GADIHLAGSRLKVKGPNR"
     gene            1087..1809
                     /locus_tag="AC16FU_08585"
     CDS             1087..1809
                     /locus_tag="AC16FU_08585"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR2994462.1"
                     /translation="MHDVIYFVSDLHLGSREDHAGGATQKKFLNFLGHIEKDASALYI
                     VGDLFDFWFEYKHVIPKVSLEVLLALRQLTGKGIALTLFFGNHDPWQIDYLQTQLPAA
                     VIPWDMTVEAQGKKIYLCHGDGLAPSDRGYRLLKKILRNKINVALYRLLPCDWAIPLA
                     RRVAGASRNYTTERGKNFLPEYEAFAQKKLNEGYDAVILGHAHEPVEKKWGGKAYLNL
                     GDWLTHFTYAELRHGTLRLKRF"
BASE COUNT          376 a          556 c          486 g          397 t
ORIGIN      
        1 gccggttcgg ccctttcacc ttcagccggc tgccggccag atgaatgtcg gcccccattt
       61 cggtcagttt ggacaccacc atgtctaagt gctccggctt gccgttgtcg atttcgacgt
      121 accccccggt caccgccgcg gccatgagga acgtggccga ttccaaccgg tccggcatcg
      181 gcgtgtactc caccgggtgc aggcggcgca cgccggtgat ggtgaccgtg gaggtgccgg
      241 cgccttccac ctttcccccg gtggcattca ggtaattggc gacatcgacc acctccggat
      301 cgcaggccgc gttgactaag gtcgtcttcc ccttggccag acaggcggcg atcatcaaat
      361 tctcggtgcc ggtgtgggtg ggacgatcaa agcaaatcgt tgtgccgact aactgccccg
      421 tcgccggcgg cacttcggcc acgatgtacc cccggtcttc ggaaagctgg gcacccagac
      481 gctgaaaccc gtagacgtgc aggttcaccg gccgcgggcc aatgacgcag ccaccgggca
      541 aagatacgcg cgctttcccc aagcgggcaa tcaaaggtcc caaaaccaga aatgaggcgc
      601 gcatcttgcg caccagatcg tacggtgctt cgtacttggt gagcgtactc gcctcgatcg
      661 aaaccgtttt ggcctggcga tcgtggttca ccttcgcgcc taactgcgca atcactttca
      721 gaaaggtgtc gatatccacc agatcgggga cgttgtggat gaccgtggtc cctttctcga
      781 ctaaaagcgc ggcggccaaa atcggcagca cggcgttttt ggaaccttcg attttcaccg
      841 agcccgaaag cggttgtcgg cccgtaatga caaatttatc catcagtgcg attcccctgc
      901 ctgttcacgt ttgcggtgca attgcaaaac gaatttacct ctggatgttc gcttgtcaag
      961 gcgcggtgct ccatacccgt ctctccttta ttctttcggc atacggcatc cacgatttta
     1021 tagccccatc cgaagcggac agtgttcact tgcaaaacgg ctctgcccta ttctacatta
     1081 cgaccgatgc acgatgtcat ttactttgta tccgacctcc atttgggctc gcgggaagat
     1141 cacgcgggcg gagcgaccca aaaaaaattc ctgaacttcc tcgggcatat tgaaaaagat
     1201 gcctccgcgc tctacatcgt tggagattta ttcgatttct ggttcgaata caaacatgtc
     1261 atcccgaagg tgtcgctgga agtgctcctg gcgctgcggc agttgaccgg gaaaggcatt
     1321 gccctcaccc tgttcttcgg caaccacgac ccctggcaga ttgattatct gcagacgcaa
     1381 ctccctgccg ccgtcatccc ctgggatatg accgtcgaag cccagggaaa gaaaatctat
     1441 ctctgccacg gtgatggttt ggctcccagc gaccgcggtt accggttgtt gaagaaaatc
     1501 ctgcgaaata agatcaatgt cgccctctac cgtctcctcc cctgcgattg ggctattcct
     1561 ttggccaggc gggtagccgg cgcgtcacgc aactacacca ccgagcgcgg aaaaaatttt
     1621 ctgccggagt acgaggcctt cgcgcagaag aaattgaacg aagggtatga cgcggtcatc
     1681 ctgggccacg cccacgaacc ggtggaaaaa aaatggggcg gcaaagccta tttgaacctc
     1741 ggcgactggc ttacccactt cacctacgcc gaactgcgcc acggcacgct tcgactgaag
     1801 cggttctgaa ctttc
//
LOCUS       DBNGZA010000396         1584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1352422, whole genome shotgun sequence.
ACCESSION   DBNGZA010000396 DBNGZA010000000
VERSION     DBNGZA010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1352422"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..621
                     /locus_tag="AC16FU_08590"
     CDS             136..621
                     /locus_tag="AC16FU_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR2994463.1"
                     /translation="MLSQDKVQLLDEMARGAEAVSAYVQHLPEEVWSFKPAPEKWSIK
                     EIVGHLADVDAKMYGPRLRRMVAENNPFLHSMDQNEFVTKGRHQEKTPRDLLAEFRQA
                     REANLAFLRQTEDKDWERTGQHEEYGRMVFSDIIRERLVPHDRKHLEQMTRNVAAFQI
                     R"
     gene            628..1578
                     /gene="arcC"
                     /locus_tag="AC16FU_08595"
     CDS             628..1578
                     /gene="arcC"
                     /locus_tag="AC16FU_08595"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR2994464.1"
                     /translation="MAAPRIAVVALGGNAITRPNEEDTIPRQFANTRASLAGIIPLIR
                     EGYRLAITHGNGPQVGNAIFRVELARGKAPDLPLGICVADTEGGMGYMIEQSLQNRLR
                     QEKVSRPVVTIITQVVVDKRDPSIKKPSKFIGQFYSEEEARRFERERNWVMKKDANRG
                     WRRVVPSPIPLEIVEKETIKQLVASGVIVIAAGGGGIPVYLEKDGSLEGVDAVVDKDR
                     ASAVLAHDISADFLLMLTGVDKVALNFGILTQKFLDRLTVTEAKKFLAEGHFPPGNMG
                     PKIEAAIQFLKSGGKEVIITSIPKAFEAVTGEAGTRIVPD"
BASE COUNT          367 a          442 c          505 g          270 t
ORIGIN      
        1 atcgtcaaaa tccgcaagcc ggcggtgtgg gtgcgctacg aactgcagga aatcgggaag
       61 cccgacttaa gagacgtcat taccgcaatg ctcgcccgga cgaaaaaagg tcgcagaagt
      121 tgaaaggagg ggtcggtgct ctcgcaggac aaagtccagt tgcttgacga aatggcgcgc
      181 ggggcggagg cggtttcggc ctacgtccag catctccccg aggaggtctg gagcttcaag
      241 ccggcgccgg agaagtggtc gatcaaagaa atcgtcggcc acttagcgga cgtggatgcc
      301 aaaatgtacg gcccgcggct gcggcgcatg gtggcggaaa acaatccgtt tctgcacagc
      361 atggatcaaa atgaattcgt caccaagggc cggcaccagg agaaaacacc ccgcgatctt
      421 ctggctgaat tccgccaggc ccgggaggcg aaccttgcct tcctacgcca gactgaggac
      481 aaggattggg aaagaacggg tcagcacgaa gagtacggcc ggatggtttt cagcgacatc
      541 atccgggaac gtctggtgcc gcacgaccgg aagcacttag agcagatgac gcgcaacgtc
      601 gccgccttcc aaatccggta aaatttgatg gctgcgccca ggatcgcggt cgtcgcctta
      661 ggcggcaacg cgatcacccg cccgaacgaa gaagacacga tcccgcggca gttcgccaac
      721 acccgcgcca gtctcgccgg gatcattcca ctcatccggg aaggataccg gctggcgatc
      781 acccacggga acggcccgca ggtcggcaac gccatttttc gggtggagct ggcccggggc
      841 aaggcgccgg acttacccct gggcatctgc gtggctgaca ccgaaggggg gatggggtat
      901 atgatcgagc agtcgctcca gaaccggctg cggcaggaaa aggtcagccg gccggtagtc
      961 accatcatca cgcaggtggt cgtcgataag cgcgatcctt cgatcaaaaa gccctccaag
     1021 ttcatcgggc agttctacag cgaagaggag gcccggcggt ttgaacgaga acgaaactgg
     1081 gtgatgaaaa aagatgccaa ccggggctgg cgccgggtgg tgccgtcgcc cattccgctg
     1141 gaaatcgtgg agaaggagac aatcaagcag ctggtcgcct ccggcgtcat cgtgattgcg
     1201 gcggggggcg gaggcattcc ggtttacctc gaaaaagacg ggtcgctgga aggggtggat
     1261 gcggtggtag acaaagaccg tgcctcggcc gttctggccc acgacatcag cgccgatttc
     1321 cttttgatgc tcaccggcgt ggacaaagtg gcgctcaact tcgggatctt aacgcagaaa
     1381 ttcctcgacc ggctgacggt aaccgaagct aaaaaatttc tggccgaggg ccacttccct
     1441 cccggaaaca tgggtcccaa aatcgaagcg gcgatacagt ttttgaaatc cggcgggaaa
     1501 gaggtgatca tcacttcgat cccgaaggcc tttgaggcgg tcaccggcga agccggcacg
     1561 cggattgttc cggattgatt tctc
//
LOCUS       DBNGZA010000397         2281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1032597, whole genome shotgun sequence.
ACCESSION   DBNGZA010000397 DBNGZA010000000
VERSION     DBNGZA010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1032597"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="AC16FU_08600"
     CDS             complement(<1..1738)
                     /locus_tag="AC16FU_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994465.1"
                     /translation="MLSLDWQLPADVCGAGKPETKGQVMRVATTFGLLSLFTSSVTFA
                     DITGSITDNTSPGLVILGAAAFEQSRVNDSLYPVLFTVDALFFVDSLPPHAFTLQTDP
                     AKTYVVAAIGFSSLSNQGGPDAVGDIHYNVRGDSSGLSLAITPNVNHITGRLLPAGGG
                     TLGNDPQVWAKDAGGTFAGVTFPDSAGNFSFNYLPGAGPYHILSLGDRHYLRVDSNKT
                     NPVNLGDLTLADAGQTFITASSNTGVPGRIRDVLSDRKNYDLTFTVYNAPSGQPLTAV
                     TFEAPGDIGENLPQPVRYYLSPNPGNHTIGPGLDWNSVTVDLSRQPHRVTFARGTGTS
                     SIPPGGSGTFTIRVDSMTNVAYDTYQGWHVAVRSNTGLQGRAQDPDSCRSGGDCGVES
                     VQKILEAYDIRFDPPGQNTGTELADIDLVLRFWVRNHGNGVFQPYANWTNVFSTSAFA
                     FQSFPTVANSIAAGESLAVLTQSVHTTVIPGSYLLFALVADSTGSNQQNSFAQYTEPL
                     FICVPLGDLAGGPGNCADGIIDLVDLVTAVNYVVFGTPFCGQVGIADVAGDPQNCPDG
                     IVDLVDLVTMVNY"
     gene            complement(1810..2133)
                     /locus_tag="AC16FU_08605"
     CDS             complement(1810..2133)
                     /locus_tag="AC16FU_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HIR2994466.1"
                     /translation="MNGGLPNKFLIGSLAADPGGVIDAGSAYVYQWFGLISAPPCVAR
                     GDVNSDGSTDIVDVVNLIQGVVSVAEGSNSKASDTNCDGTRDIADIVLLIQYVVFGTP
                     TPCCL"
BASE COUNT          505 a          679 c          676 g          421 t
ORIGIN      
        1 cgtagttgac catcgtcact aaatccacga ggtcgacgat cccgtccgga cagttctgcg
       61 gatcccccgc cacgtccgca atgcccacct gcccgcagaa aggcgtcccg aagacaacgt
      121 agttcaccgc cgtcaccaag tccacaagat cgatgatccc gtccgcacag ttacccggac
      181 cgcctgccag gtcgcctaac ggcacgcaga tgaagagcgg ttctgtgtac tgggcaaaac
      241 tattctgctg gttggagccg gtggaatcag ccaccaaggc aaaaagcagg tatgacccgg
      301 gaatgacggt ggtgtggacc gactgcgtga gcacggccag cgactcaccc gccgcaatcg
      361 aattggcgac ggtcgggaaa ctctggaagg cgaacgcact ggtgctgaaa acgttggtcc
      421 agttggcata gggctggaac acgccgttgc cgtgattccg cacccagaac cggagcacca
      481 ggtcgatgtc ggctaactcc gtgccggtgt tctgccccgg cgggtcaaag cggatgtcat
      541 acgcttccaa aatcttctgg accgactcaa ccccgcagtc tcccccgctc cggcaggagt
      601 cgggatcctg cgcccggccc tgaagcccgg tgttgctccg caccgccaca tgccagccct
      661 ggtaggtgtc gtaagcgacg ttggtcatcg aatcgacgcg aatcgtaaaa gtgcctgacc
      721 cacccggcgg aatgctgctc gttccggtcc cccgggcaaa cgtgacgcgg tggggctgcc
      781 ggctcaggtc gacggtgacc gagttccaat cgagccccgg gccaatggtg tggttgcccg
      841 gattgggcga aaggtagtag cgcaccggct ggggcagatt ctctccgatg tccccgggcg
      901 cttcgaaggt caccgcggtg agcggttggc cggagggagc attgtaaacg gtgaacgtca
      961 aatcataatt tttccggtcc gaaagtacgt cccgtatccg gcccggcacg ccggtgttgc
     1021 tgctggcggt aatgaaggtc tggcccgcgt ccgccagagt aagatcgcct aagttcacag
     1081 gattggtttt gttcgaatca acgcgcaggt agtgccgatc accaagggaa aggatatggt
     1141 acggcccggc acccgggagg tagttgaaac tgaaattccc cgccgaatcg ggaaaagtta
     1201 ctcccgcaaa ggttccgcct gcgtctttcg cccagacctg cggatcgttt cccagcgtcc
     1261 cgcccccggc gggtaaaaga cgtccggtaa tatggtttac gttgggggta atcgccaggg
     1321 agagcccgct cgaatcaccg cgcacgttgt agtggatatc acccaccgca tccggcccgc
     1381 cttgatttga gagcgaactg aaaccgattg ctgccacgac gtaggtcttg gccggatcgg
     1441 tttgcaacgt gaaggcgtgc ggtgggaggg aatcgacaaa gaacagggcg tcaacagtga
     1501 acagcaccgg gtagagtgaa tcattgaccc gtgattgctc aaaggccgcc gcgccaagta
     1561 taacgagccc cggcgaggtg ttgtcggtaa ttgagccggt gatgtccgcg aaggtgactg
     1621 aggaagtgaa aagagaaagc aggccgaacg tagtcgcaac gcgcataacc tgtcctttcg
     1681 tttccggttt ccctgcgccg cagacatctg cgggcaactg ccaatccagt gacagcatgg
     1741 gaaaaataac gtgaaggggt gaaggaaagc aagaacttct atcagggagc taagaacgtg
     1801 gaaattccgt tacaaacagc acggggtcgg cgtgccgaac acgacgtact ggatcaagag
     1861 cacgatgtcc gctatgtccc gcgtcccatc gcagttcgtg tcgctggctt tggaatttga
     1921 cccctcagcc acggatacca cgccttgaat caagttgacg acatccacaa tgtcggttga
     1981 cccgtcgcta ttcacgtccc cgcgcgcaac gcagggaggt gcgctaatca agccgaacca
     2041 ctgatacacg tacgcggaac cggcatctat gactcccccc ggatcggcag ccagtgaccc
     2101 gataagaaac ttattcggta atccgccgtt cacgttgccg gctccggcta ccgaaacccc
     2161 taaaaactcc caggcgctgc tgccgtcttt ctgggcgagt acttctcctg tggcgccgtc
     2221 aaagacaatg gctgagccgg ctgtcgataa cccccctcac gcggcaaaag gagcaccgac
     2281 a
//
LOCUS       DBNGZA010000398         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_397320, whole genome shotgun sequence.
ACCESSION   DBNGZA010000398 DBNGZA010000000
VERSION     DBNGZA010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_397320"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="AC16FU_08610"
     CDS             complement(<1..827)
                     /locus_tag="AC16FU_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HIR2994467.1"
                     /translation="MRNLLVFFLTVVLVTPAVAQDVQFKASADKPVASLSDQIAFTIT
                     ISGSGQQLPQPQLPDLSSFTVFSSGQSQNFSFVNGVMHTEVSYTYVLLPKSEGRFTIG
                     PAEITIGGRTYRTAPVTIEVSQKAVSPPAGGGQHDRYTREQVQAGLADLFLKATVDKK
                     APYVGEQVTLSLKFYQAVRLFDQPQYQAPALTGFWQEPLGNWTRSSEVVNGKHYAVHE
                     MKIALFPTQPGRQTVGSASIQCTVEDLERFFSRDPFDQFGADLRSFFNQGQTKILRT"
     gene            complement(833..1654)
                     /locus_tag="AC16FU_08615"
     CDS             complement(833..1654)
                     /locus_tag="AC16FU_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994468.1"
                     /translation="MKVIEGVYPLEVRSATLSGYGGRVFFRRNQRFPYIELLFALGAV
                     WVTLTPAPGRADTFSGAIKKGNAAYEKKEFDKALEHYRAAEIDHPESEKLQYNLGNAL
                     YKTGNFPDAAGRYEKALAKLPLTDQAKGYYNIGNALYREGKWEEAIGAYKQALELIPS
                     DEDAKYNIEFVRQKIKENLKPQQSEKQKKDQKKQPEQQKQPQAGQNPDSTGEKKPEQQ
                     PEQLAQEKKDGQKPQPKGQMSKEDAERLLQAVKEQPVQKRQTKRMFGQKATDKDW"
     gene            complement(1651..2697)
                     /locus_tag="AC16FU_08620"
     CDS             complement(1651..2697)
                     /locus_tag="AC16FU_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR2994469.1"
                     /translation="MRFGQPLILYLYILVAGLVVLLWWAGRQRRKLFSTWGDLSLLER
                     LTQTINLKSRRWKSVLLITGAGLLVFAAARPQYGQRLTYVKREGIDLLVAVDVSASML
                     AEDVKPNRLELARRDVAGLINRLQGDRIGLLAFAGVAFVQCPLTLDYGAAKLFLDVLN
                     PNLIPEPGTAIGEAIRTAQQAFVQKERKYKVLILITDGEDHDTDPIGAAKEAAREGIR
                     IYTIGIGSSDGEPIPLRGANGQLLGYKNDESGQVVVSRLDETTLQKIALETNGKYYRG
                     TTGGLELDHIYDEIAKMEKKGLKGEYQTQYEDRYQYPLFLGLLLVLVEFILPERRKIQ
                     PEVSESVKRLRMSQ"
     gene            complement(2697..>2922)
                     /locus_tag="AC16FU_08625"
     CDS             complement(2697..>2922)
                     /locus_tag="AC16FU_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HIR2994470.1"
                     /translation="LAKIAELTGGRYFSAKTEKMLSEIYKKIDELEKTKIKVKEYRQY
                     DERFWPFALGGLGLFLAEIFLANSLFRRMP"
BASE COUNT          551 a          838 c          822 g          711 t
ORIGIN      
        1 tcggtgcgga gaattttcgt ctgcccctgg ttgaaaaacg agcgcaggtc ggcgccgaac
       61 tgatcgaagg gatcgcggga aaagaaccgc tccaggtctt cgacggtgca ctgaattgag
      121 gcggagccga cggtctgcct gcccggctgg gtgggaaaaa gggcgatttt catctcgtgc
      181 accgcgtagt gcttgccgtt gacgacctcg cttgagcgcg tccagtttcc caacggttcc
      241 tgccagaagc cggtgagggc gggcgcctgg tactggggct ggtcaaagag ccgtaccgcc
      301 tggtagaatt tgagcgagag cgttacctgt tccccaacgt agggcgcctt tttatccacg
      361 gtggccttca aaaaaagatc ggccaggcca gcttggactt gctcacgcgt gtagcggtcg
      421 tgctggccgc cccccgccgg aggagacacc gctttctggc tgacctcgat ggtgacgggc
      481 gcggtgcggt aggtcctgcc gccgatggta atttctgccg gcccgatggt gaagcgccct
      541 tccgacttgg gcaaaagaac ataggtatag ctcacctcgg tatgcatcac accgttgacg
      601 aacgagaaat tttgcgactg cccggaagaa aagacggtaa agctcgagag gtcgggcaat
      661 tggggttgcg ggagttgctg cccactgccc gaaatagtga tggtaaaagc aatctggtca
      721 gaaagcgagg ccaccggctt gtcggccgac gccttgaatt gaacgtcttg agccactgcc
      781 ggcgtgacca gaacgacggt taagaaaaaa acgagaaggt tccgcatacg ttctaccaat
      841 ctttatcggt cgccttctgg ccgaacatcc gcttggtctg ccgtttctgc accggttgct
      901 ccttgacggc ttgcagcagg cgttctgcat cttctttgct catctgcccc ttgggttgcg
      961 gcttttgccc gtcttttttt tcctgcgcca actgttccgg ctgctgttcc ggtttcttct
     1021 ctccggtgga atcagggttc tggcccgcct gcggctgctt ttgctgttcc ggctgttttt
     1081 tttggtcttt cttctgcttt tcggactgct gcggcttgag attttcttta atcttctggc
     1141 gtacaaattc gatgttgtac ttcgcgtctt cgtcgctcgg aatgagctcc aaagcctgct
     1201 tatacgcccc gatggcctcc tcccacttcc cttcccggta gagcgcattg ccgatgttgt
     1261 agtacccctt cgcctggtcg gtcaggggga gtttggccag cgctttttcg taccggcctg
     1321 ccgcgtcggg gaagttaccg gtcttgtaaa gtgcattacc caggttgtac tgaagttttt
     1381 ccgattcggg atggtcgatt tccgccgccc ggtagtgctc cagcgccttg tcgaactctt
     1441 ttttctcgta ggcggcgttc cctttcttga tcgcgcccga aaaagtatcg gccctgcccg
     1501 gtgccggggt gagcgttacc cagactgccc cgagagcaaa aagtaactca atgtaaggga
     1561 atcgctggtt gcgccgaaag aaaacccggc ctccgtaccc cgatagggtt gccgacctga
     1621 cctccagggg gtacacccct tcgatcactt tcattgactc atcctcaacc gttttacgct
     1681 ttcggaaact tccggttgga tcttgcgccg ctccggaagg ataaattcaa cgagtaccaa
     1741 tagaagtccc aggaaaagcg ggtactggta gcggtcttcg tactgggtct ggtactcccc
     1801 tttcaaacct ttcttctcca tcttggctat ttcgtcataa atgtggtcca attccagccc
     1861 gccggtggtg ccgcggtagt acttgccgtt ggtttcgagt gcaattttct gcaaggtcgt
     1921 ctcgtctaaa cggcttacca ccacctggcc ggattcgtca ttcttatacc ctaagagttg
     1981 tccgttcgct ccccgcaggg ggattggctc tccgtcggat gagccgatgc caatggtgta
     2041 tatccgaatc ccttcgcgag ccgcctcttt ggccgcgccg attggatcgg tgtcgtggtc
     2101 ctcgccgtcg gtgatgagga tcagcacctt gtacttccgt tctttctgga cgaaggcctg
     2161 ttgagcggta cggatggctt cgcctatggc cgtccccggt tcgggaatga ggttcgggtt
     2221 caggacgtcc agaaacagct tggccgcgcc gtaatccaag gtcaacggac actggacgaa
     2281 cgcgaccccg gcaaaggcca aaagcccgat ccggtcaccc tgcaggcggt tgattaatcc
     2341 ggctacgtcg cgccgggcca gttccaggcg gttcggtttc acatcttcgg ccagcatcga
     2401 ggcggagaca tcgactgcga ctaaaagatc gatgccctcg cgcttgacgt aagtgagcct
     2461 ctggccgtac tggggtcgcg ccgcggcaaa gacgaggagg ccggccccag ttatcagaag
     2521 cacgctcttc cagcggcggc tcttcagatt gatcgtctgg gtcaggcgct ccaggagcga
     2581 aagatcgccc caggttgaaa agagctttcg tctttgccgt cccgcccacc agagcaaaac
     2641 caccagcccg gcgaccagaa tgtacaagta gagaatcaga ggttgtccga aacgcactac
     2701 ggcatcctcc ggaaaagcga attggccaaa aatatttccg ccaggaaaag ccccagcccg
     2761 cccaaagcga aaggccagaa gcgctcgtcg tactggcggt attctttgac tttgatcttc
     2821 gtcttttcta attcgtctat tttcttgtaa atctcagaaa gcattttttc ggtcttagcg
     2881 ctgaagtagc gcccgccagt caactccgca attttcgcaa gc
//
LOCUS       DBNGZA010000399         2358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2412763, whole genome shotgun sequence.
ACCESSION   DBNGZA010000399 DBNGZA010000000
VERSION     DBNGZA010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2412763"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="AC16FU_08630"
     CDS             complement(<1..1026)
                     /locus_tag="AC16FU_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994471.1"
                     /translation="MAGDDTPHHLHYDYRDIFIAPRLALSGKKILGHFIGLVIGYGGY
                     VFFTYLALISLGESFDAVWANFGLAPYLPLEDVPWFGALLAVLGLLWLLLAWLVSSFT
                     VAKVTFEELRGNLFYSLREGWAFARTNLKKLFAPFFFSVLFVVALLLLLVFAGLFSRI
                     PWLGELSFSLFFLVPLFAFAAVVVVTLVGLAMGVLLLPAAVVAEEKGSFEATAHLFSL
                     LWRQPWRWSLYTGLSGLLAKVATFVFGYACFRAAGLVADLASLLGGDKIDFLIDKALE
                     YLPAEANLVLFFSTLFPGLDFGFHLPIAEGTPDLPVVQEIAGLVLGLGFVTLFLAVVA
                     YGLAILSS"
     gene            complement(1095..1742)
                     /locus_tag="AC16FU_08635"
     CDS             complement(1095..1742)
                     /locus_tag="AC16FU_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR2994472.1"
                     /translation="MRNNKRISESTIHRLSLYYRSLNALESEGYATISSRELAKRERL
                     TPAQVRKDLSFFGTFGTRGLGYPVHELKEKISAIMGLNRTWNVAVVGAGNIGSALVAY
                     REFSRQGFSIRAIFDNDRRKIGSTHEGVTVSDSKNLTPELKEKNIDIVILAVPAPVAQ
                     VVTDDIVAAGVRAILNFAPVRLKVPDDVILRNENMSIELEHLSFALMNRNSPPRG"
     gene            1954..2040
                     /locus_tag="AC16FU_08640"
     tRNA            1954..2040
                     /locus_tag="AC16FU_08640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1988..1990,aa:Ser,seq:gga)
     gene            2163..2321
                     /locus_tag="AC16FU_08645"
     CDS             2163..2321
                     /locus_tag="AC16FU_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2994473.1"
                     /translation="MSENRDYWFVYLLRCADGSIYTGITNDVEARVREHNQDIVGLTP
                     AMPWFILL"
BASE COUNT          568 a          652 c          712 g          426 t
ORIGIN      
        1 ggaggagagg atcgccagac cgtaggcgac gacagccaaa aacaacgtca cgaagcccaa
       61 gcccaaaacc agtccggcaa tctcctgcac cacgggcagg tccggcgtgc cttcggcaat
      121 cgggaggtga aacccgaagt ccagtccggg aaacaaagtc gaaaaaaata agaccagatt
      181 ggcctcggcg ggcaggtact ccagggcttt gtcgatcaga aaatcgatct tgtccccgcc
      241 gagaagcgac gccaggtcgg ccaccaggcc ggcggcgcgg aagcaagcgt aaccgaaaac
      301 aaacgtggcg accttcgcca agagtccgga aagtccggtg tagagactcc agcgccaggg
      361 ctgccgccag agaagcgaaa agaggtgggc cgttgcttcg aacgaacctt tctcttcggc
      421 caccactgcg gcggggagca ggagcactcc catagctaac ccgaccaagg tcaccacgac
      481 caccgccgca aaggcgaaaa gcggaaccag gaagaataac gaaaaggaga gttctcccag
      541 ccagggaata cggctgaaaa ggccggcgaa aaccaaaagc agcaggaggg cgactacaaa
      601 gagcaccgag aagaagaacg gcgcgaagag ttttttgaga tttgtccggg caaaggccca
      661 gccttcgcgc agggaataga acaggttgcc ccgcaactct tcgaaggtga ccttcgccac
      721 ggtaaaactc gacacgagcc aggccaaaag gagccagagg aggcccagga cggccaggag
      781 cgccccaaac cagggtacgt cttccagcgg gagataggga gccagaccga agttggccca
      841 gacggcatcg aacgactcgc ccagagaaat cagcgccagg taagtgaaaa agacgtaccc
      901 gccgtagcca atcaccaagc cgatgaagtg ccctaagatt ttcttgcccg aaagggctaa
      961 acgcggcgca atgaagatgt cgcggtaatc gtagtgcagg tggtgcggcg tgtcatcccc
     1021 ggccatcggt ctgcctccgt ccctcccgga gacagtatag gtcggggggg aatctgtcaa
     1081 gccgaaaaga gggctcaccc gcgcggcggc gagttgcggt tcatcagggc gaaggaaagg
     1141 tgctccagtt cgatcgacat gttctcgttc cggaggatga cgtcgtcggg caccttgagc
     1201 cgaaccggcg caaagtttaa gatggccctc accccggcgg ccacgatgtc gtcggtcaca
     1261 acctgcgcca ccggcgccgg caccgccagg atcacgatgt caatgttttt ctcttttaat
     1321 tccggggtca gatttttgct gtcggagacg gtcacccctt cgtgggtgga accgatcttg
     1381 cgccggtcgt tgtcgaagat cgcccggatg ctgaatccct gccggctgaa ctcccggtag
     1441 gcgaccagag ccgagccgat gttccccgcg cccaccaccg ccacgttcca ggtgcggttc
     1501 aaccccatta tggcggagat cttttccttc aactcgtgca cgggatatcc cagaccgcgg
     1561 gtgccgaacg tcccgaaaaa cgaaagatct ttccgcacct gcgccggggt caaacgctcc
     1621 cgcttggcca attcgcgcga tgaaatggtg gcgtacccct cgctttccag tgcgttgagt
     1681 gagcggtagt agagggagag ccggtgaatg gtcgattcgg atatgcgctt gttattccgc
     1741 acgccatacc tcttgtgaac tctttcacaa tataactgag gaaggtcttt cgtcaataga
     1801 aaatttcagt ggcgggcttg cctttggggc ggtttcgagg tcacaatcac cggcccaatt
     1861 tgcatgtttt aggagaggcg ggatcgctcg aggcagagag ccgtgcacgt cttgttagag
     1921 ccaaccactc ggcttaaatt gagacagtcg tccggagaga tgtccgagtg gctgaaggag
     1981 catgcctgga gagcatgtag ggtcgtaagg ccctcctggg ttcgaatccc agtctctccg
     2041 ctgtaaagcc ctgccatcgg ctctgaagaa ccttcaggct ctgaacgaga gcgaagtcga
     2101 agggtttatc ccgagtgagt ctcgtcccgc cacaggcggg agacgaatcg agggattgat
     2161 cgatgtccga gaacagagac tattggttcg tctacttgct ccggtgcgcc gatggttcta
     2221 tttatacggg gatcaccaac gatgtagagg cgagagtgcg ggagcataac caggacattg
     2281 tcgggttgac cccagcaatg ccgtggttta ttttgttgta gaggaggtga gtcgattatc
     2341 ttgattacag aaaatgct
//
LOCUS       DBNGZA010000400         2863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2623291, whole genome shotgun sequence.
ACCESSION   DBNGZA010000400 DBNGZA010000000
VERSION     DBNGZA010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2623291"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC16FU_08650"
     CDS             <1..402
                     /locus_tag="AC16FU_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994474.1"
                     /translation="SLMYIYIHEKRYDAALALADSLSREYPESRSFLWGQAAAYFESY
                     HWAEAARVYVELVRRVEEEPDQNYYNLIDCTYRIAQCRFNLGRFNDCITSCEKLLAYP
                     ASPEIVKRQEAKIRKTRALLEKAQKITGKKK"
     gene            complement(406..1368)
                     /locus_tag="AC16FU_08655"
     CDS             complement(406..1368)
                     /locus_tag="AC16FU_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994475.1"
                     /translation="MNTKPRFLFPGLGRALAAQRLLFFLWLANLVCAAALTYPLRAAF
                     ADAAGTSLAGRTLGEGFNFALMSDFIEHRGAALDAAFGLLGPALLFFLFLYTFFDGGI
                     IAQFNNDQNRPLLSSFLANCGKYWGRLLRLFLWFLPAVIVFYIITKIMSKIAGLVSAN
                     RGNEHLTIWAYGVAWVLSFGVWCFFSMIFDYAKIGLVVEDSRKTFAALRRSFRFVFRN
                     FGRTVRLYFSLVAIGLVFFLIYFLIEINAPTEKWGIFWLVLILQQLCLLSRIWLRLGF
                     YSCQTALYLRYADRTTSETLPAPVFTGTAAPPIPSAGERPESAA"
     gene            complement(1369..>2863)
                     /locus_tag="AC16FU_08660"
     CDS             complement(1369..>2863)
                     /locus_tag="AC16FU_08660"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR2994476.1"
                     /translation="GRKTGGWNCHQFHANSEFYADYGSYRVTLTVPDGFKVGATGART
                     SESADPKAHTQTVTYLQEDVHDFAWTASPAFVVQPDTHVFPSGHRVAITLLNQPDYAG
                     QKQRLLEAAHQALKYFGRWYGEYPYATLTIVMPPTGAFGAGGMEYPTLFTGITLVPAD
                     WPLLRNIHYLVEETIVHEFGHQYWYGLVGSNEFEESWLDEGLNTYSTGKVLAAWFGDK
                     ASFGRCLGAAISELEFARWQVVQYPHYDPVNTFSWRFFNGNSYGTNSYAKVQILLTTL
                     ENYLGPDRMENVMRTYFERFRFKHPATEDFVRTANEVSGQNLDWFFNQFVYGTQKVDY
                     AVAGIASQEIKPQRGFFDLDGRKVFVESGGDTTVLGDTALTKGKTLAKAKDRNGKKKE
                     VKVYESAVQIQRKGDGIFPVEIEIKFEKEKPFTYHWDGEERWIKYRFRGPHELEYAAV
                     DPQNKIVLDLNRLNNSRTIVKQSRAITRWSSRWLFVMQNLLQTLAVF"
BASE COUNT          667 a          816 c          814 g          566 t
ORIGIN      
        1 tccttgatgt acatttacat tcacgagaaa cgctatgacg cggcgctggc cttagccgac
       61 agtctttccc gcgagtaccc ggagagcagg agtttcctct ggggccaggc cgccgcgtac
      121 ttcgaaagct accattgggc cgaggccgcg cgggtgtacg tcgaactggt gcgcagagtt
      181 gaagaggagc cggaccagaa ctactacaac ctgatcgact gcacctaccg gatcgcccag
      241 tgccggttca acctggggcg gttcaacgac tgcattacct cctgcgaaaa acttttggcc
      301 tacccggctt ctccggagat agtcaaaaga caggaagcga aaattcgcaa gacgcgcgcg
      361 cttttggaga aagcgcagaa aatcaccgga aagaagaagt agactttagg ccgccgactc
      421 gggccgctct cccgcagagg gaattggagg agcggccgtg cctgtaaaga caggagctgg
      481 caatgtctct gacgttgtgc gatcagcgta tcgtaagtag agggcggttt ggcatgagta
      541 aaatcccaac cgcagccaga tgcggctcaa aaggcacagc tgctggagga tgagcaccag
      601 ccagaaaatg ccccatttct cggtcggtgc atttatctca atcaagaagt aaatcaggaa
      661 aaagacgaga ccaatagcca ccaaggagaa gtacagccgg accgtccggc caaagttacg
      721 gaagacgaag cgaaacgacc ggcgcaaggc ggcaaacgtc ttccgggaat cttccaccac
      781 cagccctatc ttggcgtagt cgaagatcat cgaaaagaag caccagacgc cgaaggagag
      841 cacccaggcc accccgtacg cccagatggt gaggtgttcg ttgcctctgt tcgccgaaac
      901 cagaccggcg attttgctca tgattttggt gatgatgtag aatacgatta cggccggcaa
      961 aaaccagagg aaaagccgca ggagcctccc ccagtacttt ccgcaattag ccagaaacga
     1021 agaaagaagg ggcctgttct gatcgttgtt gaactgggct ataatgccgc cgtcaaaaaa
     1081 ggtgtaaagg aaaaggaaga acaagagtgc ggggccaagc aggccgaagg cggcgtcgag
     1141 cgccgcgccc cggtgttcga tgaaatcgct cataagcgca aaattaaaac cctcccccag
     1201 tgtccgcccc gccaaacttg tccccgcggc atcggcaaat gccgcccgca gggggtaggt
     1261 gagcgccgcg gcgcaaacca aattcgccag ccagagaaaa aacagaaggc gctgggccgc
     1321 gagcgcccgg cccaagccgg ggaaaagaaa tctcggcttt gtgttcatct agaacaccgc
     1381 caaggtttgc aacaaattct gcatcacaaa aagccaccgg gaggaccacc gggtgatcgc
     1441 ccgcgactgt ttcactatcg tccgactgtt gttgagccga ttaagatcga ggacgatttt
     1501 attctgcgga tcaaccgccg catactccag ttcgtgcggc ccccggaaac ggtacttaat
     1561 ccagcgctct tccccatccc agtggtaggt gaacggcttt tccttctcga acttgatttc
     1621 aatctccacc ggaaagatac catccccttt ccgctggatc tgaactgctg actcgtagac
     1681 ttttacttct tttttcttgc cgttcctgtc cttcgctttc gcaagcgtct tgcctttggt
     1741 cagagcggtg tcgcccaata cggtggtatc cccgccgctt tccacgaaca ccttccggcc
     1801 gtcgaggtca aaaaagcccc gctggggctt gatttcttgg ctcgcaatgc cggccacggc
     1861 gtaatccacc ttctgggtac cgtagacgaa ctggttgaag aaccaatcca gattttgccc
     1921 ggaaacttcg ttggccgtgc gaacgaaatc ctccgtggcg ggatgcttga accggaagcg
     1981 ttcgaagtag gtgcgcatca cattctccat ccggtcgggg ccaaggtagt tctccagggt
     2041 cgtgaggagt atctgaacct tggcatagga gttagtgccg taactattac cgttgaaaaa
     2101 ccgccaggaa aaggtattga ccgggtcgta gtgcgggtac tgcaccacct gccagcgggc
     2161 gaattctaat tcgctgatcg cagcacccag gcagcggcca aaactcgcct tatcgccgaa
     2221 ccaggccgcc agcaccttgc cggtggagta ggtgttgagc ccttcatcca accacgattc
     2281 ttcgaactcg ttggagccga ccagcccgta ccagtactgg tggccgaatt catggacgat
     2341 ggtctcctcc acgaggtagt gaatgttgcg caaaagcggc cagtcggccg ggacgagcgt
     2401 tatgccggtg aaaagcgtgg gatactccat cccgccggcg ccgaaagccc ccgtgggcgg
     2461 catcacgatg gttaaagtcg cgtagggata ctccccgtac caccggccaa aatatttcag
     2521 cgcctggtgg gccgcctcca ggagcctttg cttttgaccc gcataatcgg gttggttcag
     2581 gagcgtgatc gccactctat gtccgctcgg gaaaacgtgc gtatctggct gcaccacaaa
     2641 ggccggtgaa gcggtccagg caaagtcgtg aacatcttcc tgcaagtagg tgaccgtttg
     2701 cgtgtgcgct ttcgggtctg ccgactcact ggtgcgggcg ccggtggccc cgactttgaa
     2761 gccgtcgggc acggtcaggg tcacccggta cgagccgtag tcggcgtaga attcgctgtt
     2821 ggcgtggaac tgatggcaat tccagccgcc ggttttgcgt ccg
//
LOCUS       DBNGZA010000401         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2623427, whole genome shotgun sequence.
ACCESSION   DBNGZA010000401 DBNGZA010000000
VERSION     DBNGZA010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2623427"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="AC16FU_08665"
     CDS             <1..639
                     /locus_tag="AC16FU_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2994477.1"
                     /translation="WELKGKPTTVTFTLSEKGAATEVTLIHDLSAFAGDRWHWYLLSD
                     HWHLALPNLLYFVETGQPAYRVDFSKARGNVRLDIDIIASAEKIFQSLTVPQEMDKWL
                     SEKAEVELKVGGRYSFGWTIIVDGKKVPGGPTTITEIIPNRRLVHGWYWPGEDTGSTE
                     VTWQLESRGKKTVVQLKHTGFVADRDGTDYYQGWSAFLCRLKYLLERGKSWE"
     gene            736..1425
                     /locus_tag="AC16FU_08670"
     CDS             736..1425
                     /locus_tag="AC16FU_08670"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR2994478.1"
                     /translation="MRRVILAVFLGLLILAGVVYAQVSKQEAAPAGKTPWHLKATIIE
                     ACSCPLFCPCYFNLKPAHHYCQFNNAFNIEQGNLGNVKLDGMKVWLSGDLGGDFTTGK
                     AEWLVFTFEPTATPEQIDAGTKVLGSLYSAFKFPVLGIDKKQITWEIKGNKATAKMGD
                     GSGSIDLVAATGSDGKTPTVINNLPYWSAKSNSGFNLYKSKHHYKGHEKDFTFEDTNG
                     FTIGIETSGEM"
     gene            complement(1502..2794)
                     /locus_tag="AC16FU_08675"
     CDS             complement(1502..2794)
                     /locus_tag="AC16FU_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR2994479.1"
                     /translation="MKTPTEKKLIYDWNAAVPKKRPAYNRKVTLADETLRDGLQSPSI
                     IDPPIATKIKILHLMENLGIQSCDIGLPGSGGRARADITELACEISRRRMKIKPFCAC
                     RTVIADIEPLLEISEKAGYPLEPAIFIGSSPIRIYAEEWTLDRMLQLTEEAVSFAVKH
                     GLPVMYVTEDTTRAHPSVIKKLYTTAIECGARRITVCDTVGYVTPVGIANLIAYVRKV
                     VKATGEKVKIDFHGHRDRGLDIPNALSAIAAGVNQVHATALGIGERVGNCAMDLLLVN
                     LKLEGVIKNDLSKLNEYCRTVAEAVGMPIPINYPVIGRDAFRTATGVHAAAVIKAKKK
                     GDHYLANRVYSGVPAELFGRQQEIEIGFMSGASNVNYWLIQRGIPPTPELVATILQTA
                     KDHNKILTEAEVLEVVHRFGPGRTAPASATFGSRSQFA"
     gene            complement(2859..4007)
                     /locus_tag="AC16FU_08680"
     CDS             complement(2859..4007)
                     /locus_tag="AC16FU_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR2994480.1"
                     /translation="MEFELTENQKVVRDLAREFAQKKLFPRAEEFDAREELDLALVRE
                     MGELGFLGLAIPEEYGGGGLDYLALVLAVEELSQGCASHTAVVSLHNSLFAHPVLAFG
                     TEEQKKRFLPPIARGEKFCAFALTEPGAGSDAGALETSARGEGDFYVLNGNKVYVTDG
                     NLADFILVYATVDRSLGHKGITAFMVEKTMPGFAVGTREKKMGYKASPTYELSFTDVR
                     VPKENIIGMVGEGFKIAMDSLNGGRIVIGAKALGLAEGAYRLALKYAKERRQFGQPIA
                     NFQAIQFMLADMATQIEAARALIYHAARLKDHGLPHTLEGAQAKLYASEMATRVTHKA
                     MQILGGYGYLRDYQVERYYRDARVTEIFEGTSEIQRLVIARNLLKGKA"
     gene            complement(4056..4205)
                     /locus_tag="AC16FU_08685"
     CDS             complement(4056..4205)
                     /locus_tag="AC16FU_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994481.1"
                     /translation="MHAKSEQMDLAPLPHQDKVDLFEILGETGHLPEWDNETTPAELD
                     SRQKR"
     gene            complement(4224..>4573)
                     /locus_tag="AC16FU_08690"
     CDS             complement(4224..>4573)
                     /locus_tag="AC16FU_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994482.1"
                     /translation="PSGTSRLATEKPQITITDQFQKRISGGYYGLTEDSILLHVSTRL
                     GLRRIWVDDVTHLKVKGKSQRGTGVLLGFLAGVAVAAIIDIQRIQRSTEENFTDPAFP
                     LKAAIAATIGGTV"
BASE COUNT          977 a         1337 c         1295 g          964 t
ORIGIN      
        1 tgggagttaa agggcaaacc gaccacagtt acgttcactc tctcagaaaa aggcgctgct
       61 accgaagtca ccctgatcca cgacctctct gccttcgccg gtgatcggtg gcactggtac
      121 ctcttgagcg accactggca cctggccttg ccgaatctcc tctacttcgt cgaaactggt
      181 cagccagcgt accgcgtaga tttttccaaa gcgagaggaa acgtgcgctt ggatattgat
      241 attatagcct cggcggagaa aatttttcag tccctcaccg ttccacaaga aatggacaaa
      301 tggctttctg aaaaagcgga agtggaattg aaagtcggcg ggcgctactc tttcggctgg
      361 acgataatag tcgatgggaa gaaggtcccc ggcggcccga cgacaatcac cgagatcatc
      421 ccgaacagaa gactcgtgca cggctggtac tggcccggcg aagacaccgg ttccacagaa
      481 gtaacctggc agctcgaatc gcgcggcaaa aaaacagtgg tgcaattgaa gcataccggc
      541 tttgtggctg accgcgacgg aaccgactac taccagggct ggagcgcctt cctctgccgg
      601 ctcaagtacc tgttggaacg agggaaaagc tgggagtaga gccccccaaa aaagttgttg
      661 acaaattcca gagcgataac gtattctttc ccgtcgtgga aaagcagaat aaaccttaac
      721 cacaaggagg gatcgatgcg cagagtaatt ctggccgtct tcctcggact gctcattctg
      781 gcaggcgttg tctatgccca ggtgagcaaa caggaagcgg cccccgcggg caaaacgccc
      841 tggcacctga aggcgacaat tatcgaggcc tgctcgtgcc cgcttttctg cccgtgctac
      901 ttcaacctca agccggccca ccactactgc cagttcaaca acgccttcaa cattgagcag
      961 gggaacctgg ggaacgtgaa gttggacggg atgaaagtct ggctctccgg cgacctgggc
     1021 ggcgacttca ccaccggcaa ggcggagtgg ctggttttca cctttgaacc gacggccacc
     1081 ccggagcaga tcgacgccgg aaccaaggtg ctgggctcgc tctactcggc cttcaagttc
     1141 ccggtcttag gaatcgacaa gaagcagatc acctgggaga tcaaggggaa caaggcgacc
     1201 gccaagatgg gcgacggcag tggttcgatt gaccttgtcg ccgccacggg ctccgatggc
     1261 aagaccccga cggtcatcaa caacctcccc tactggtcgg ccaagagcaa cagcggcttc
     1321 aacctgtaca agtccaagca ccattacaag gggcatgaga aggatttcac cttcgaggac
     1381 accaacgggt ttacgatcgg gatcgaaacc tccggagaaa tgtaaacccg gggcgtacgc
     1441 ctccaaagga gagggcaacc tctccttttt attttagtct caaggagtgg ggaatgacgg
     1501 cttaagcgaa ctgcgatcga ctgccaaagg tagccgaggc gggcgcggtg cggccgggac
     1561 cgaaccgatg cacgacttcc agcacctcgg cttcggttaa aattttattg tgatctttgg
     1621 cggtctggag aatggtggcg acgagttccg gcgtcggcgg gatgccgcgc tggatgagcc
     1681 agtagttgac gttcgaagcc ccgctcatga aaccgatttc tatttcctgc tggcggccga
     1741 agagttccgc cggtaccccg gaatagacgc ggttggcgag atagtggtcg ccttttttct
     1801 tggctttgat caccgccgcc gcgtgcacgc cggtggcggt gcggaaggcg tcccgcccga
     1861 tgaccgggta gtttatcgga atcggcatcc ccaccgcttc ggccaccgtg cggcagtatt
     1921 cgtttagttt cgacaggtcg tttttaatca cgccttctaa tttcaaattc accagaagca
     1981 aatccatcgc gcagttcccc acccgctcgc cgatgccgag cgcggtggcg tggacttgat
     2041 tgaccccggc ggctattgcg gaaagagcgt tggggatatc caaaccccgg tcacggtggc
     2101 cgtggaagtc gattttgacc ttctcgccgg tggctttgac cacttttctg acgtaggcaa
     2161 tcagattggc aatccccacc ggcgttacgt atcccaccgt gtcgcagacc gtgatccgcc
     2221 gcgccccgca ctcaatggcg gtggtatata acttcttgat aactgaggga tgcgcccggg
     2281 tggtgtcctc ggttacgtac atcaccggga ggccgtgctt gaccgcaaaa ctcaccgctt
     2341 cttcggtcaa ctggagcatg cggtcaagcg tccactcttc cgcgtagatg cggatgggcg
     2401 aagagccgat gaaaatcgcc ggttccagag ggtagccggc tttttcggaa atctccaaaa
     2461 gcggctcgat gtcggcaatg accgtgcggc aggcgcagaa tggcttgatt ttcatccggc
     2521 ggcgcgaaat ttcgcacgcc aattcggtaa tatcggcgcg cgcccggccg cccgaaccgg
     2581 gcaaaccgat gtcgcacgac tgaatgccta agttttccat caggtggagg atttttatct
     2641 tggtggcgat gggcggatcg atgatcgagg gtgactggag cccgtcgcgc agggtctcgt
     2701 ccgctagggt gactttccgg ttgtaggcgg gacgcttttt cggaactgcg gcgttccagt
     2761 cgtaaatcag cttcttttcg gtaggcgttt tcattgcggt tcatcaaatc tacggttcgt
     2821 agaaaagaca agcaagtaac tgtctcggtt aacagtcgtc acgcttttcc cttgagcaaa
     2881 ttcctggcga tcaccagccg ttgaatttcg gaagtcccct cgaaaatctc ggtcacccgc
     2941 gcatcacggt agtagcgctc cacctggtag tcgcgcaggt agccgtaacc gcctaaaatc
     3001 tgcatcgctt tgtgcgtcac gcgggtggcc atttccgagg cgtacagttt cgcctgggcg
     3061 ccttccagcg tatgcggcaa accgtgatcc ttgagccggg ccgcgtggta aatcaaggcg
     3121 cgcgccgctt caatctgggt cgccatatcg gccaacatga actggatggc ctgaaaattg
     3181 gcgatcggct gaccgaactg ccggcgctct ttggcatatt tgagcgccag tcgatacgca
     3241 ccttcggcca gacccaaagc cttggcccca atcacaatcc ggccgccgtt gagggaatcc
     3301 atcgcgatct tgaatccctc tccgaccatt ccgatgatat tttccttcgg gacgcgcacg
     3361 tcggtgaacg aaagctcata agtcggcgaa gctttgtagc ccatcttttt ttcccgcgtc
     3421 cccacggcaa aacccggcat tgtcttctcg accatgaaag ccgtaatacc cttatgcccg
     3481 agcgaacggt caaccgtggc atagacgaga atgaagtcgg ccagattccc gtcggtgacg
     3541 tagactttat tcccgtttaa gacataaaaa tctccctccc cgcgagccga agtctccaga
     3601 gcccccgcat cggacccggc gccgggttcg gtgagggcga aagcgcagaa cttttccccg
     3661 cgcgcgattg gcggcaagaa acgctttttc tgctcctcgg tcccaaaggc cagaaccggg
     3721 tgagcgaaga gcgaattgtg gagcgacacc accgccgtgt gggaagcgca accctggctc
     3781 agttcctcca cggctaagac taaagcgaga taatccaggc cgccgccgcc gtactcttcg
     3841 ggaatggcga ggccgaggaa gcccagctct cccatttccc gcaccaacgc taagtcgagt
     3901 tcctcccggg catcgaattc ttcggcgcgg ggaaaaagct ttttctgcgc aaactcgcgc
     3961 gccagatcgc gcacgacctt ctgattttcg gtaagttcaa attccatgtg gtttggactg
     4021 tccgtctccg ccaagaagtc tctcaggacg actgcctatc gcttttggcg cgaatccagt
     4081 tcggctggag tcgtttcgtt atcccactcg ggaaggtgcc cggtctcgcc cagaatttca
     4141 aacagatcaa ctttatcctg gtggggcaac ggtgccagat ccatctgttc ggatttcgcg
     4201 tgcaccgctg agccgagggc gtatcacacg gtgccgccga tggtggccgc gatggccgcc
     4261 ttgagcggga aggccggatc ggtaaaattc tcctcggttg accgttggat gcgctggatg
     4321 tctatgatcg ccgccaccgc tacccccgcc agaaaaccca gcaatacacc cgttcctcgc
     4381 tgcgatttcc ctttgacctt aagatgggtg acatcgtcca cccaaatccg ccgcagaccc
     4441 agccgggtgg aaacgtgcag gaggatgctg tcctccgtca gcccgtagta accgccagaa
     4501 atgcgcttct ggaactggtc ggtgatggta atctgcggct tctccgtcgc cagacggctg
     4561 gtgccggacg gca
//
LOCUS       DBNGZA010000402         4640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_182719, whole genome shotgun sequence.
ACCESSION   DBNGZA010000402 DBNGZA010000000
VERSION     DBNGZA010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_182719"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16FU_08695"
     CDS             <1..287
                     /locus_tag="AC16FU_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994483.1"
                     /translation="DPLAFYFEVYNLRRGPDGKTHVKVGYEVVDFKSKKKIVTPLPQA
                     LVSDSTDLVRTGTVDIKTLPPGDYILAVRVVDETTRKEKSALASFRIVKG"
     gene            521..2302
                     /locus_tag="AC16FU_08700"
     CDS             521..2302
                     /locus_tag="AC16FU_08700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR2994484.1"
                     /translation="MTIDTEKTEGATVTYRGPAHAHACLFRDVAKIVSSELEIDALVQ
                     VTLELLVKTFNAGAGALHLFHPETGKVHLYVSDQDGNRAPREISYRPGEGFLGWVAEK
                     REPLLVNDTTHDLRSAPVLESEFVSPVRSLVVVPLVWRDNLYCVLELINKVEGDFTSE
                     DRDTLGALADQIGIAFRNASLYEKLKKESLQRERLFEIGKYLSSSLELDEVLEKILDA
                     LHQVVPYSVAGIFLVDRKSQTVNTFHTRGYDPALAADLRLKIGQGLVGYVAKTGEPVI
                     VPDVAVDPRYINAHSETQSEMVAPIVAGGKVIGVFNLEANRKAAFSPADLELLNAFAG
                     QAALTIERARLYREILGKKRLEDELKIARQIQVSFLPTKNPTVRGFDIAGLNISSQQV
                     GGDYYDFIPIVEGQMGIAIADSIGKGIPAALVMASFRASLLAEIRNNYAIRTILGKVN
                     RLLWESLEQGNFVTAVYGVLDTQNRILTYSNAGHNQPILRRASGKIDYLAEGGMALAI
                     SPDAAYAERRFTLRAGDILVFYTDGVTEAKNERNEEFGQGRLEALLHQPPNLSAEALA
                     HKIVDEVKMFAAPTHQMDDLTLVVVRA"
     gene            2454..>4640
                     /locus_tag="AC16FU_08705"
     CDS             2454..>4640
                     /locus_tag="AC16FU_08705"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HIR2994485.1"
                     /translation="MTKKFKASSIFLLLFGTLLWGGVTLYLNLTLKKSFSITYDGATW
                     KTASGGLVIDSLVPGDPVDRAGLLRTDTLLLANGVSVTSDPALQDVLDRTPAGRPATY
                     TVRRGADLVKLEVPVYRYRSEIIWHWLLQLAGFVYIVLAVLVYAKKPESKTARTFFFF
                     GLVAGSLVATIWVNEPSPNKYFDLFTNVWLYYNIFFINALGLDFIRRLMFFSPGQEPK
                     KRLLLLNYLPALLGLALYLAPLVLSLAHGQFVIKAPVGFWNKIQKIIFPATYGLYLVW
                     FFAEIATRARKKQFAINRTQGEWLRWGVGLPMALFVVLEILLPAFFKIELIKWSRVIL
                     LPPPFVFAYLILKERILDVGVVIKRSAIYAILSALLIGTFVVLVMAVSQLAVLVTGQE
                     SKLAVFASALLTAGVFNLYRQKTQNYLDRKFFKDRHNYQKALLEFSKELSWLEELETL
                     LAKVSKQFAEALHLYTCLPFIYDQKADAYVMVAPYGLSDPALQQVCYPASRFGLATLL
                     AQERRSLEFYALETDPLYTYLPVVEKVALKKTDTALAVPLLVKEKLVGMLHLGNKKSG
                     DQFNSEDIDFFATLSAPLAVAVENARLYQQEVEKREMERELVVARQIQQKLVTRELPK
                     MPGVDLAAAYLPSRQVSGDIYCILPVRENQIGLAVGDVSGKGVPASLLMATVRASLIT
                     LVEEGLPPPRMMSRLNRLVYENTEPEHFVTFFYAVYDRVTRKLSYVN"
BASE COUNT          984 a         1312 c         1297 g         1047 t
ORIGIN      
        1 acgatccctt ggcgttctac ttcgaagtgt acaatctccg tcgggggccg gacggcaaaa
       61 cccacgtcaa agtgggctac gaagtggtgg atttcaaaag caaaaagaaa atcgtgacgc
      121 ctttgcccca ggctctggtt tccgacagca ccgatttagt ccgcaccggc acagtcgaca
      181 tcaagacgct gccgccgggc gactacatct tggccgtccg cgtggtggat gaaaccaccc
      241 gcaaggagaa gtcagccctg gccagtttcc ggattgtgaa aggctgaaag aattaagcat
      301 tgcgcggtgg ggattgaggg gggaagcgct cgtgcattcg ttgtgagctc taaaacgttt
      361 cttcaactcc cgacttcccg ctaaatttcc tccgagaatc tgtcgagaga aatacgagag
      421 ggtgctccag tcgtgttccg gacatttcga attgacagtt ctactttgca aaaataagtt
      481 acgaactccg gcaaaaggaa aacgtatacc ttaagtagtg atgactatcg acacagaaaa
      541 aacagaaggt gcgaccgtca cctaccgggg accagcgcac gcccacgcct gtttattccg
      601 ggacgtggcc aaaatcgtca gttcggagtt ggaaattgac gcgctggtgc aggtgacgct
      661 cgaacttttg gtcaagacct tcaacgccgg tgccggtgca ctccaccttt tccacccgga
      721 gaccggtaaa gtccacctct acgtctccga ccaggacggt aaccgtgcgc caagggaaat
      781 tagctaccgg cccggggagg gattcctcgg ctgggtggcc gagaagcgcg aaccgctcct
      841 ggtcaacgat accacccacg acctacgttc cgccccggtt cttgagtctg aattcgtctc
      901 tccggtgcgc tcgctggtgg tcgtaccctt agtctggcgc gacaatctgt actgtgtcct
      961 ggaactcatt aataaggtcg aaggagactt tacctcggag gatcgggata ccctgggggc
     1021 cttggcggat cagatcggca ttgcctttcg caacgcctcc ctctacgaaa agttgaagaa
     1081 ggaaagcctg cagcgcgagc ggctgtttga aatcggcaag tacctgtcgt cgtctctgga
     1141 gttagatgaa gtcttagaga aaatcttaga cgcgctgcac caggtcgtac catacagcgt
     1201 ggcgggtatc ttcctggttg accggaagag tcaaacggtc aacactttcc acacccgcgg
     1261 ctacgatccg gctttagccg ccgatctccg gctcaagatc ggccaggggc tggtcggcta
     1321 cgtggccaag accggcgaac cggtgatcgt gcccgacgtg gcagtggacc cgcgctacat
     1381 caatgcccac tccgaaacgc agtcagagat ggtggccccg attgtggccg gcggaaaggt
     1441 gattggagtc ttcaacctcg aagcgaaccg gaaagccgca ttcagtccgg cagatctgga
     1501 gttgctcaac gcttttgcgg gtcaggcggc cctgaccatc gagcgggcgc gactctaccg
     1561 ggaaattttg ggcaagaagc gcctggaaga cgaactgaaa atcgcccggc agatccaggt
     1621 ctcgtttctg ccgacgaaaa accctaccgt caggggtttt gacatcgccg gactgaacat
     1681 ctcctcccaa caggtgggcg gcgactacta cgacttcatt cccatcgtcg aagggcagat
     1741 gggcattgcc atcgccgatt cgatcggcaa ggggattccc gcggcgttgg tgatggcctc
     1801 cttccgcgcg tcgcttttag ctgaaatccg caacaactac gccatccgca ccatcctggg
     1861 gaaggtgaac cggctgttgt gggaatcgct ggaacaaggg aatttcgtca ccgcggtcta
     1921 cggcgttctg gacacccaga accgcatcct cacctactcg aatgccgggc acaaccagcc
     1981 gattctccgg cgggcgagtg gcaagatcga ctacctggcc gaagggggaa tggcgctggc
     2041 gatttctccg gacgccgcct acgccgagcg ccgcttcacc ctgcgcgcgg gggatattct
     2101 ggttttttac accgacgggg tgaccgaagc caaaaacgag aggaacgagg agttcggcca
     2161 gggacggtta gaagcactcc tgcaccagcc tcccaacctc tcggcggagg ccctggccca
     2221 caagatcgtc gacgaagtca aaatgttcgc cgccccgacg caccagatgg acgacctgac
     2281 gctcgtggtc gtccgcgcct gaagagcttt ccctgcgaaa agtttctctt tccggctttg
     2341 ggtttgtata tttttgccgt gatcgagaga agtcttgcgc gagggtgggg aacgtacacg
     2401 ggggtgtgtc ccgcaagcca attgatccct ccggcggccg gtaatttctc accgtgacca
     2461 aaaagtttaa agcctcgtct atttttctcc tcctctttgg aaccttgctc tggggtgggg
     2521 ttaccctcta tttgaacctg accctgaaga agtctttcag tataacgtac gatggtgcca
     2581 cctggaaaac cgcttccggg ggcctggtca tcgattccct tgtcccgggt gacccggtgg
     2641 atcgagccgg cctcctccgt acggacactt tgctattggc gaacggtgtc agcgtgacct
     2701 ccgatccggc tctgcaggac gtcttagacc ggactcccgc cggccggccg gccacctaca
     2761 ccgtccggcg cggagctgat ttggtaaagt tggaagttcc ggtgtaccgg taccgatcag
     2821 agatcatctg gcactggctt ttgcaattgg cggggttcgt ctacatcgtt ctggcggttc
     2881 tcgtctatgc aaagaagccg gagtcaaaaa cagcacgtac cttcttcttt ttcggtctgg
     2941 tcgccggctc cctggtggcg accatttggg tgaacgagcc cagtccgaac aaatacttcg
     3001 atctcttcac caacgtttgg ctctactaca acattttttt catcaatgcc ctcgggctcg
     3061 atttcattag acggcttatg tttttttcgc ccggtcaaga gcccaagaaa aggctattgc
     3121 tcttaaatta tcttcccgct ctgctcggac tggccttgta tttggcaccc ctggttctct
     3181 ccctggccca cggccaattc gtcattaaag cacccgttgg tttttggaac aaaattcaga
     3241 aaataatctt tccggccacc tacggtctct atttagtctg gttttttgcc gaaattgcca
     3301 ctcgcgccag gaaaaaacag ttcgccatca atcgcactca gggggaatgg ctgcggtggg
     3361 gagtgggcct gccgatggca cttttcgttg ttttggaaat tctcctgccg gcttttttca
     3421 agattgagct tataaagtgg agccgggtca tccttttgcc cccccctttt gtcttcgcct
     3481 acttgatatt gaaagagcgg attttggatg tcggcgtggt gatcaagcgc tcggccatct
     3541 acgccatcct gagcgcgctt ttaatcggca cctttgtcgt tctcgtgatg gccgtaagcc
     3601 agctggccgt gctcgtaacg gggcaagagt cgaaactggc ggtgtttgcc agcgcactcc
     3661 taactgccgg ggtgttcaat ttgtaccggc agaaaaccca aaattatctc gaccggaaat
     3721 tcttcaaaga ccgccacaac tatcagaaag cactcctcga attctccaag gaactctcat
     3781 ggctggaaga attggaaacg ctcctggcca aagtatccaa gcagtttgcc gaggcactcc
     3841 atctgtacac ctgcctgccg ttcatttacg accagaaggc ggatgcctac gtgatggtgg
     3901 ctccgtatgg gctctccgac ccagccctgc aacaggtgtg ctacccggct tcccggtttg
     3961 ggctggcaac gctcttggct caggaaagac ggtcgctgga attttacgcg ctggaaaccg
     4021 atccgctcta tacctacctg ccggtggtcg aaaaggtggc tttgaagaag accgatactg
     4081 ccttggcggt accgcttctg gtcaaagaga aattagtcgg gatgctccac ctcggcaaca
     4141 agaaatcggg tgaccagttc aactccgagg acattgattt ctttgccacc ctttcggcgc
     4201 ctttggcggt ggcggtggaa aacgcccggc tctaccaaca ggaagtggaa aagcgcgaga
     4261 tggaacggga gctggtggtc gcacgccaga tacagcagaa actggtgacc cgcgaactgc
     4321 cgaaaatgcc gggagtcgat ctggcggcgg cctacctccc ctcccggcag gtttccggcg
     4381 atatttactg tatcctgccg gtgcgagaaa atcagattgg cttggccgtg ggcgacgtct
     4441 ccggtaaggg ggtgccggct tcgcttttga tggccacggt acgcgcttct ctcataacgc
     4501 tggtggagga gggcctgccg ccaccccgga tgatgtcccg tctgaaccgg ctggtgtacg
     4561 agaataccga gccggagcat ttcgtgacgt ttttttatgc cgtttacgac cgggtcacca
     4621 gaaaattgag ctatgtgaac
//
LOCUS       DBNGZA010000403         2251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_292023, whole genome shotgun sequence.
ACCESSION   DBNGZA010000403 DBNGZA010000000
VERSION     DBNGZA010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_292023"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16FU_08710"
     CDS             complement(<1..544)
                     /locus_tag="AC16FU_08710"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994486.1"
                     /translation="MKTSRKIETSLFLLLVLLAGSGCVYYNTFYYARRAYNKAYQEQK
                     KQGQLKANPAQSRQYEEAIQRASKVLANHPRSSWVDDALLLIGKSYFYEGEYQKAERT
                     FRELIAGFPKSKFVPEAQFFIGWSLVLSGSYDNAMTELQRTLGLTKNKDLLGRVEMQL
                     AETYYIQEEYDSAIVHFQTLV"
     gene            complement(541..1314)
                     /locus_tag="AC16FU_08715"
     CDS             complement(541..1314)
                     /locus_tag="AC16FU_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994487.1"
                     /translation="MPEITIPANLPSVEQKLLRSFVAGLQLTFADRLESAVLYGSAAR
                     GRYIQKRSNLNTLVILQSVHLGELETYLAVRKKIEKPPLLPPLLLDHNHIRSSVDVFP
                     IEFLDMQKHHVTLCGADPFTDLTIDRKSLRAQCERELKGKLLRLRQVFLEAGHHWLGL
                     EQLLTGSFASLRPTLAALVYLASGTYPQDETALLTEVAATYKVVGDGLRRVAGLKTEK
                     PHRDRSKYQALFADYLNILTELCGKVDTMGHVKTSGHEL"
     gene            complement(1314..2072)
                     /locus_tag="AC16FU_08720"
     CDS             complement(1314..2072)
                     /locus_tag="AC16FU_08720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR2994488.1"
                     /translation="MRFSQTLAGLLRSDKNVVALTGAGVSAESGLTTFRGQDGLWKKF
                     KPEELASFAAFQKNPQIVWEWYQHRREVLTNAKPNPGHYALAAFENHFPNFTLVTQNV
                     DGLHRRAGSKNVLELHGNIQRNRCLGCGQITDAIDLPSEEIPPRCPCGGRLRPDVVWF
                     GELLPEKTLKQAFKAAEQAQLFFTIGTSAVVQPAAALPIVAAEHGAYVVEINPEETEI
                     SHLVAESLRGTSAAILTELATHLGVPVSENERVT"
BASE COUNT          476 a          590 c          679 g          506 t
ORIGIN      
        1 ccacgagtgt ctggaagtgg acgatggcgc tgtcgtattc ttcctggatg tagtaggttt
       61 cggccagctg catttcgacc cgacccaaga ggtctttgtt tttcgtgagg cccagggtgc
      121 gttgcaattc ggtcatggcg ttgtcgtagc ttcccgacag gaccaaagac cagccgatga
      181 aaaactgggc ttccggcacg aatttggact tggggaagcc tgcgatcaat tcacggaagg
      241 tgcgctcggc cttctggtac tcgccttcgt agaagtaact cttcccgatc aataataagg
      301 catcgtccac ccacgaggag cgggggtggt tggccagcac tttggaagca cgctggatgg
      361 cctcctcgta ctgccgggac tgggccgggt tggctttgag ctgcccctgc tttttctgct
      421 cctggtaggc cttgttatac gcacgtcggg cataatagaa cgtgttatag tagacacaac
      481 cgctgcctgc cagcaagacg agcaggagaa aaagagaagt ctcaattttc cgagaagttt
      541 tcaaagttca tgcccgctgg tcttcacgtg tcccatggta tcaaccttcc cgcacagctc
      601 ggtcaggata ttgagatagt cggcaaacag ggcctggtac ttgctgcggt cgcggtgcgg
      661 cttttcggtt ttgagtccgg ccacccggcg caggccgtct ccaacaacct tatacgtggc
      721 ggcgacctcc gtcagcagcg cggtttcgtc ttgcgggtag gtgccggaag cgaggtagac
      781 caaagctgcc agcgtcggcc ggagcgacgc aaacgaaccg gtcaggagtt gttccaaccc
      841 cagccagtgg tggccggctt ccaggaagac ctgccgcaga cggagcagtt tgccttttag
      901 ttccctctcg cattgggcgc ggaggctttt ccggtcaata gtcaggtcgg tgaagggatc
      961 ggcaccgcag agcgtcacgt ggtgcttttg catgtcgaga aattcaatcg ggaatacatc
     1021 caccgaggaa cggatgtggt tgtggtcaag caacagaggc ggcagcaggg gtggtttttc
     1081 gatctttttc cggacagcca aataggtttc cagttccccc aggtgcacgc tctggaggat
     1141 gaccagggta ttcaagttcg agcgtttctg aatgtatctc ccgcgggccg cgctgccata
     1201 gagcaccgca gactctaaac gatcagcgaa ggttagttgc agtccagcca caaaagaccg
     1261 cagtagtttc tgctcaaccg aagggagatt ggccggaatg gtaatctcgg gcatcaggtt
     1321 accctttcat tttccgaaac gggtactccc aggtgtgtcg ccagttcggt cagaatcgca
     1381 gcggaggtcc cgcgcagcga ctcggccacc aggtgggaga tttcggtctc ttccggattg
     1441 atttccacga cgtaggcgcc atgctcggca gccacaattg gcaacgcagc ggccggctgg
     1501 acgacagcgg aggtgccgat ggtgaagaac aattgggcct gttcggcggc cttgaatgct
     1561 tgtttcagag tcttttcggg gagcagttcc ccgaaccaaa cgacgtcagg ccgcaggcgg
     1621 cctccacagg ggcaacgcgg gggaatttct tcggagggga ggtcaatggc atcagtaatc
     1681 tgaccgcatc ccagacaacg attgcgttgg atgttcccgt gtagttcgag gacgttttta
     1741 ctccccgccc ggcggtggag gccgtcgacg ttctgggtga cgagcgtgaa gttcgggaag
     1801 tggttttcga aagcggctaa ggcgtagtgc cctgggttcg gcttggcgtt cgtcagcacc
     1861 tcccgccggt gctggtacca ctcccagacg atttgcggat ttttctggaa agcggcaaac
     1921 gatgccagtt cctccggctt gaattttttc cagagcccgt cctgcccgcg aaaggtggtg
     1981 aggcccgatt ccgccgagac gcccgcaccg gtgagggcca cgacgttttt gtccgacctg
     2041 agtaatcctg ctaaagtttg agaaaaacgc attggaaatt acccttcttc agaaatatac
     2101 gtctgaagag tggaaaaacg aaagccatat agtaaggaag gagggaatag ccgccaggct
     2161 gaaaagcctc accactaagc acgcgggaaa gaggatttat acttggagat aatacttctg
     2221 agttcctgcc aatagagatc cattttggtt g
//
LOCUS       DBNGZA010000404         5116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2730751, whole genome shotgun sequence.
ACCESSION   DBNGZA010000404 DBNGZA010000000
VERSION     DBNGZA010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2730751"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC16FU_08725"
     CDS             complement(<1..650)
                     /locus_tag="AC16FU_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR2994489.1"
                     /translation="MSKSISVGVIGVGHLGRHHARIFAALEGVVLAGLYDRDYPKTKR
                     VAGELGVPAFANLQALFDQVQVVSIVVPTTAHAEIACAALACGKDVFIEKPISATLPE
                     ADRIIALSQEKQALVQVGQIERFNPCFAALENFPLDPLFIEVHRLAGFSPRGLDVAVV
                     LDLMIHDIDLVCHLVKAPLENVQAASAAVISDSDDIANARLVFANGCVANLTASRIS"
     gene            complement(683..1483)
                     /gene="lpxA"
                     /locus_tag="AC16FU_08730"
     CDS             complement(683..1483)
                     /gene="lpxA"
                     /locus_tag="AC16FU_08730"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR2994490.1"
                     /translation="MTIETEIFAPPRIHPTAVVNPRAQIAEDVLIGPYAIIGRDVEID
                     SGTTVGSHALVDEGARIGKSVQIHHGAVVSGRPQDLKYQGEPTHLFVGDGTIIREFAT
                     LHRGTTHTGKTVVGLNCLIMAYAHVAHDCRIGDNVILANAVNLAGHVTIEEWAIVGGV
                     VPVHQFVRIGAHAMIGGGFRVPMDVVPYALAGGYPLKIEGLNKIGLKRRGFPEPTIKA
                     LRQAFRILFQAKMNTTPALERIRAEVEHLPEVQRLVRFVAESERGITK"
     gene            complement(1530..2927)
                     /locus_tag="AC16FU_08735"
     CDS             complement(1530..2927)
                     /locus_tag="AC16FU_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HIR2994491.1"
                     /translation="MNQKQRTIKSPVSFSGVGLHTGNQATIAFKPAPANTGIVFVRTD
                     LPGRPKVPADIDWVSDITRGTTLAKGDVKIYTVEHVLAAAAGLEIDNLSVEMDNNEPP
                     VGDGSALPYVEKLLQAGIVEQDEPKVFLEIDQPLAYSEPSRGVDLVVLPSDDFRITFM
                     IDYRNPALGTQYTTLASLEQEFVKEFAPARTFCFLHEVEELKKLGLIKGGSLDSAIVI
                     CEDGVAASDLKRLKKLFGDGRDLSVGKNGILNDTTLRFPNEPVRHKAIDLLGDLSLIG
                     MPLKAHVLAARSGHAANVALARKIRGLYQKEKIIKKYQAQKAAKDSVLDINTILRLLP
                     HRYPFLLIDRIIDLEPEQRVVALKNVTINEPFFAGHFPGHPIMPGVLIIEAMAQAGGF
                     LLLQTVEEPEKKVVYFIGIDKVRFRRPVLPGDQLRFEVTMLAYRHNTCKMEGKAFVGD
                     ELVTEAMLMATVVDR"
     gene            complement(2902..3159)
                     /locus_tag="AC16FU_08740"
     CDS             complement(2902..3159)
                     /locus_tag="AC16FU_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994492.1"
                     /translation="MTTPYDRVAAVAPELSRKASQVAEEILAFFFEPEELTDEKYENY
                     AFVIENIVAEYLESSASRYLAARFTREMEQNRTDEPKAKDN"
     gene            complement(3165..3731)
                     /gene="liaF"
                     /locus_tag="AC16FU_08745"
     CDS             complement(3165..3731)
                     /gene="liaF"
                     /locus_tag="AC16FU_08745"
                     /inference="COORDINATES: protein motif:HMM:NF021443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HIR2994493.1"
                     /translation="MAQSKGGTFIGLLLVLIGFLFLLNSLDLVDFGRSLGILWPLLLI
                     GVGIWFMLRHRRRPGTTVNPEDHRSTMQLFGDVGYTANGRVPERLTVSILFGDAQIDL
                     TRGEFPYPTHYIDHSSVFGDLKIRVPPSLAVSVSGHTLFGDTDVFGKKSGGFAQNLEL
                     AGGAAAGDARRLVISTNVVFGDVEVRTP"
     gene            4269..>5116
                     /locus_tag="AC16FU_08750"
     CDS             4269..>5116
                     /locus_tag="AC16FU_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR2994494.1"
                     /translation="MHAFQPASAEKFKFQEIIYEKKDWVARVTINRPESYNAYSTQAL
                     KELIAAFQDASWDDAIGVVVFTGAGDKAFCTGGDVKEYASVYTKKPRDYWKYMGLFRA
                     YLESILNLGKPTIARINGMAVGGGNESQMACDLAIMAEHAYLGQIGTNVGSVACGGAT
                     QWLPILIGDRRARYMLLLNERIPAKQALEWGLVNQVVPYEKLDAAVSDMCQKLLAKFP
                     ECTRYTKAQVNFWKELSWHNTIGHARDWLALHFTSLEPHEGMQAFVEKRKVDYMRLRK
                     LAAEGKS"
BASE COUNT         1090 a         1456 c         1462 g         1108 t
ORIGIN      
        1 gaaatccggc tggcggtgag gttggccacg cagccgttgg caaagaccag ccgggcgttg
       61 gcgatgtcat cggagtcgga gattaccgcg gcagaagcgg cctgaacgtt ctcgagaggc
      121 gctttgacca ggtggcagac taaatcgatg tcgtgaatca tgagatcgag caccacggcc
      181 acatccagcc cgcgggggga gaaaccggcc agccggtgca cttcgatgaa gagcggatcg
      241 agcgggaagt tctcgagcgc cgcaaagcag gggttgaagc gctcgatctg tcctacctgg
      301 actaaggcct gcttctcttg cgacagcgcg atgatccggt cggcttccgg cagggtggcg
      361 ctgatcggct tctcgatgaa aacgtccttc ccgcacgcca gagcggcgca ggcgatctcg
      421 gcatgagcgg tagttggaac gacaatgctc actacctgga cttgatcgaa gagtgcctgg
      481 agattggcaa aagcgggaac gcccagctcg cccgccaccc gtttcgtttt gggataatcg
      541 cggtcgtaga ggccggccag gaccacccct tccagcgcag cgaaaatgcg ggcatggtgc
      601 cggcccaggt gcccgacacc gatcacgccg acggaaatcg attttgacat agcggccgaa
      661 accggaacag gtccggaaaa aactacttgg ttatgccccg ttcgctttct gccacaaagc
      721 ggaccagccg ctggacttcc gggagatgct cgacttccgc ccggatgcgt tccagagccg
      781 gcgtcgtgtt catttttgcc tgaaagagaa tccggaaggc ctgccggagg gctttaatcg
      841 tcggttcggg gaagccccgc cgcttgagtc ctattttgtt gagtccttcg attttgagcg
      901 gatacccacc ggccaaagcg tacggcacca cgtccatggg gacgcggaag cccccgccga
      961 tcatggcgtg ggcgccgatc cgtacgaact ggtgcaccgg caccaccccg ccgacaatcg
     1021 cccactcttc tatggtgaca tggccggcca agttgaccgc gttggccagg ataacgttgt
     1081 cgccgatccg gcagtcgtgg gccacgtgcg cataggccat gatcaggcaa ttgaggccga
     1141 cgaccgtctt gcccgtgtgc gtcgttcccc ggtgcagggt ggcgaactcc cggataatcg
     1201 tgccgtcacc cacaaagagg tgggtcggct cgccttgata cttcaaatcc tggggccggc
     1261 cgctcaccac cgcgccgtgg tgaatctgga cgctcttccc gatgcgggct ccctcatcca
     1321 ccagtgcgtg ggagccgacc gtggtgccgg aatcaatttc aacgtcccgc ccaataatgg
     1381 cgtacgggcc gatgagcaca tcctccgcaa tctgcgcccg cgggttgacc accgccgtgg
     1441 gatgaatcct gggcggggcg aaaatttccg tttcaatggt cattgtggtc tttccctacc
     1501 accttccacc tacggcccat cacctgttct tatcgatcca cgacggtcgc catgagcatc
     1561 gcttcggtga ccagttcgtc gccgacgaag gccttgcctt ccatcttgca ggtgttgtgc
     1621 cggtacgcga gcatcgtcac ttcgaaccgc aactggtcgc cgggcaaaac cgggcggcgg
     1681 aagcgcactt tgtcgatgcc tatgaagtag accaccttct tctccggctc ctcgacggtc
     1741 tggaggagga ggaacccacc ggcttgcgcc atcgcctcga tgatgagcac ccccggcatg
     1801 atcgggtgac ccggaaagtg cccggcgaag aacggctcgt tgatggtgac gtttttgagc
     1861 gccaccaccc gctgctccgg ttccaggtca atgatccggt caatcaagag gaacgggtag
     1921 cggtgcggca gcaaccgcag aattgtgttg atgtctaaga ctgaatcttt ggcggctttc
     1981 tgcgcttgat atttcttaat aattttttcc ttctggtaca gcccgcggat ttttcgggcc
     2041 aaagccacgt tggccgcgtg gccggaacgg gccgccagaa cgtgcgcttt gagcggcatg
     2101 ccgatcaggg agaggtcgcc cagaaggtcg atggccttat gccgcaccgg ctcgttggga
     2161 aagcgtaacg tcgtatcgtt caggatgccg ttcttgccga cgctcaagtc ccgcccgtcc
     2221 ccgaataact tcttcaaccg cttcaaatcg gacgctgcca ccccgtcctc gcaaatgacg
     2281 atggcggaat ccaaactccc gcctttgatc agccccagct tcttcagttc ctccacctcg
     2341 tgcagaaaac agaaagtccg cgcgggggcg aactctttga cgaattcttg ctccagcgat
     2401 gctaacgtgg tgtactgggt gcccagggcc ggattgcggt agtcgatcat gaaggtgatc
     2461 cggaagtcgt ccgagggcaa aacgaccaga tcgacgcccc gggagggctc ggagtaggcc
     2521 agcggctggt cgatctccag aaaaaccttc ggctcgtcct gttcgacgat gccggcctgc
     2581 agcagtttct ccacgtaggg caacgccgac ccatccccca ccggcggctc gttgttgtcc
     2641 atctcgacgg agagattatc gatttccagc ccggccgccg cggccagaac gtgctccacg
     2701 gtgtagattt tcacgtcgcc tttggcgagc gtggtgccgc gggtgatgtc cgagacccaa
     2761 tcgatatccg cgggtacctt tggcctcccc ggcaggtcgg tgcggacgaa gacaatgccg
     2821 gtgttcgccg gagccggctt gaaggcgatc gtcgcctgat tgccggtgtg cagcccgacc
     2881 ccggagaaag ataccggaga tttaattgtc ctttgctttt ggttcatccg tgcggttctg
     2941 ttccatttcc cgggtgaatc gggcggctaa gtaccggctg gcgctggatt ctaagtactc
     3001 cgccacgatg ttctcgatca cgaacgcata gttctcgtac ttttcgtcgg tcaattcttc
     3061 cggctcgaag aagaacgcga gaatctcctc cgccacctgt gaggctttcc ggctcaattc
     3121 cggtgctacg gctgctaccc ggtcgtaggg ggttgtcatt agtctcatgg cgtccgcacc
     3181 tccacgtcgc cgaaaacgac gttggttgaa atcacaagcc ttctggcgtc accggctgca
     3241 gctcctccgg ccagttcgag gttctgggcg aatcctcccg acttctttcc gaagacgtct
     3301 gtgtcgccga agagcgtgtg gccggaaacc gagaccgcaa gcgacggcgg caccctaatt
     3361 ttcagatcgc cgaagaccga actgtgatca atataatgcg tcgggtaagg aaattcaccc
     3421 cgggtgaggt ctatttgggc atctccgaag aggatggaaa ccgtcaagcg ctccggtacc
     3481 cttccgttgg cggtgtaccc cacgtcgcca aaaagttgca tggttgagcg gtgatcttcc
     3541 ggattgaccg tcgtacccgg tcgccgtcgg tgacggagca taaaccagat ccctacgccg
     3601 atgagcaaaa gcggccagag gatgccgagc gagcggccaa agtcaaccag gtcgaggctg
     3661 ttcaggagaa aaaggaagcc aatgagaacc aaaagcaagc cgatgaacgt tcccccttta
     3721 ctctgtgcca ccagtttctc ccttaattgt tttacctacc acctgacttt tcacaaccac
     3781 agtatcaatc ctgcgaacgg cactgcacct ctcctacacc tgccgggact taccctaagt
     3841 cctttaacag cttgggagat cacatttgaa agataaggtt agaccgggga cttttgcaac
     3901 ggcaggttgc ggcccaagtc ggctcaagcg agcattttct gagtcaccat aacgtcattt
     3961 tggttgatat ctttctggag ttgccgcagc ttcctcggtg gccagttgaa gataattgac
     4021 tcgctgtaac gactgcatgc tctgaggaac ctaagaacat taacaaccct aaaaggcggg
     4081 caaataaccg cggaataccg gttccgcgtc gcggcgattc ctctgttgac ttcacgtttc
     4141 ccctcccgta aattcgccgc gtggactctg tcggcagaaa gccggccatt tactcccaaa
     4201 ttcctacctc aatcgttatg gagatagatt tttaaggacg gtttgtgtcc ggaaagcgag
     4261 gattttgaat gcatgcattt cagccagctt cggcagaaaa gtttaaattc caagaaatta
     4321 tctatgagaa aaaagattgg gtggcaagag ttacgataaa ccgccccgaa tcatacaacg
     4381 cctatagcac tcaagcttta aaagaattga tcgcggcttt tcaggatgca tcctgggatg
     4441 atgctattgg agtggtagtt tttaccggag caggagacaa agcgttttgc accggcggag
     4501 atgtgaaaga gtacgcatcg gtttacacga agaaaccgcg cgactactgg aaatatatgg
     4561 ggcttttccg ggcctatttg gaaagtattc tcaacctggg caaaccgacg attgcgcgca
     4621 tcaatggtat ggccgtgggt ggtggtaatg aatcccagat ggcctgcgat ctggctatca
     4681 tggccgagca cgcgtacttg ggccaaatcg gaaccaatgt cggctcggtc gcttgcggcg
     4741 gtgctacgca atggctgccg attttgatcg gcgaccggag agcccggtat atgcttttat
     4801 taaatgagcg aataccggcg aaacaggctt tagaatgggg tttagtaaat caggtggtcc
     4861 cttatgaaaa acttgacgcc gcggtctccg acatgtgcca gaagcttttg gctaaatttc
     4921 cagaatgtac gcgttatacg aaagctcagg tgaacttctg gaaagaactc tcctggcata
     4981 acaccatcgg tcatgcgcgc gactggctcg ccttgcactt cacttcctta gaaccccatg
     5041 aggggatgca ggctttcgtg gaaaagagaa aggttgacta tatgcggtta agaaaattag
     5101 ccgctgaagg taaatc
//
LOCUS       DBNGZA010000405         3448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_825518, whole genome shotgun sequence.
ACCESSION   DBNGZA010000405 DBNGZA010000000
VERSION     DBNGZA010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_825518"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="AC16FU_08755"
     CDS             <1..1419
                     /locus_tag="AC16FU_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2994495.1"
                     /translation="GDITSIRPLMFITDPIMGRVLYADATRTWIKALNLYEPWGITTD
                     NDEDNDDFMNVYVTEASIAEVRKLFFCLAPDCDRLNKGLLLSMPADGLQHPVDVTLNQ
                     GADGFGGNFSDYLWVLDDRTCKLYLYEPFGFAGARYQYGGPGYGVGGFWKPTAVACGR
                     NPIDGLHTKTVYVADNPGPGQRLVELNGTSGARTPLQWGKVWVPNSSSWIDDIAVDSY
                     GHLWLADAINHRLIKISPYDFSTLGTFGSKGTGPNQFLFPCTVGHPEGKEGWADLFVG
                     EEWGPNSGGQYYVMGVDAKDLQTEVSGVNVGGSVGLEFYYEDLLADPAYITENIYQWN
                     PNTSQWVFVARPRNNVTLYSGVTPSSAVVFPGTIGTVDYKIQIYATSTYHNQSGVPVN
                     TDTLEAIVSACSPPLRGDVTLDCVIDISDLTTLVNYVVYSTPMPAGPSMGDANCDGVV
                     DIVDIIYLLNYITFGEPVPCPF"
     gene            complement(1435..2433)
                     /gene="galT"
                     /locus_tag="AC16FU_08760"
     CDS             complement(1435..2433)
                     /gene="galT"
                     /locus_tag="AC16FU_08760"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR2994496.1"
                     /translation="MPELRKDPVVGRWVIISTERSRRPTDFTPVPRKSESKNCPFCPG
                     NEAATPPEILAFRPDKSARNTPGWTVRVISNKYPALRIEGDLNREGVGIFDKMNGVGA
                     HEVIIETPDHTKELAHLSPQEVSDVLWAYRERMVDLKNDPRFRYLMIFKNQGEAAGAS
                     LEHAHSQLIALPIVPQQVLEELEGAKRYYDYKERCIFCDGIRQDIADGERVVGQTEHF
                     VALEPFAPRYPFETWILPRFHDAAFERLNPKQFPELAGVLRDILLRLERALANPPFNY
                     ILHTSPVDGDHQKEYHWHFEIMPTVTRVAGFEKGTGFFINPTSPEEAAQYLNEIKI"
     gene            complement(2577..>3448)
                     /locus_tag="AC16FU_08765"
     CDS             complement(2577..>3448)
                     /locus_tag="AC16FU_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HIR2994497.1"
                     /translation="AQRQLVDRLPRKPFKGRWTNYLWELVAANGDEIARDFAALAPAL
                     DFKKMFDRCEVDQQTVIHKVEQVYLGEGAKIDAGTVLDARSGPIFIDRQVTIQAQTRV
                     EGPAYIGPRSILVGGRIRSGSSVGPVCRIGGEVEQSIFLGHDNKYHDGFLGHAYVGQW
                     VNLGALTTNSDLKNNYGTVRVEVAGREVDTGLLKVGSFIGDHVRTGIGTLLNTGISIG
                     FCSNLYGGGMFAQKSIPPFSWGTSGKIDEYQVDQALLTAEQVQRRREVAFSPAQKRLF
                     LELFEQFRAGKRR"
BASE COUNT          684 a         1019 c          964 g          781 t
ORIGIN      
        1 ggagatatta cttctattcg tccgctgatg ttcataaccg acccgatcat gggtcgtgtt
       61 ctttacgccg atgctaccag aacctggatc aaagcgctca acctgtatga gccgtggggt
      121 atcaccaccg acaacgacga agacaacgat gactttatga atgtctatgt gaccgaagcg
      181 agcattgccg aagtgcgtaa gctctttttc tgcctagccc ccgattgtga ccgactgaac
      241 aaaggccttt tactttcaat gcccgccgat ggccttcagc acccggttga tgttacgctc
      301 aaccaaggtg ctgatgggtt tggtggtaat ttttctgatt atctctgggt tctggatgac
      361 cgcacgtgca agttgtacct ctacgagccc tttggttttg cgggtgctcg ctaccagtat
      421 ggtgggccag gctacggcgt cggtggtttc tggaagccca ccgctgtagc ttgcggacga
      481 aatccgattg acggactgca tacaaagacc gtctacgttg ctgataaccc cggtcctggc
      541 cagcgcttgg tggaattaaa cggcacatct ggcgctagaa ccccgttgca gtgggggaag
      601 gtgtgggttc cgaatagctc atcttggatt gatgacattg cagttgattc ctacgggcat
      661 ctctggctgg ctgacgccat aaaccaccgg ctgataaaaa tttcgcctta cgatttcagt
      721 acactgggga ctttcggctc caagggtacc ggtcccaacc aattcctctt cccttgcacc
      781 gtgggccatc ctgagggcaa agagggctgg gccgaccttt tcgtgggcga ggagtgggga
      841 ccaaacagcg gcgggcagta ctacgtgatg ggcgtggatg caaaagattt gcaaaccgag
      901 gtcagcggcg tgaacgtcgg aggcagcgtg gggttggaat tctactatga agacctatta
      961 gccgacccgg cctacattac ggagaatatt taccagtgga acccaaacac gtcccagtgg
     1021 gttttcgttg ctcgtccgcg aaataatgta actctctaca gcggcgttac accctcctca
     1081 gcagttgtgt tccccggtac gataggcaca gtcgattaca aaattcaaat ctatgcaacc
     1141 tcaacctacc acaatcagag cggcgtcccg gtcaataccg atacgctcga agcaattgtc
     1201 tcggcatgct ctcccccgct ccgcggcgac gtcaccctcg attgcgtcat cgacatctcc
     1261 gatctcacca cgctcgtcaa ctatgtcgtc tacagcaccc ccatgcccgc cgggcccagc
     1321 atgggcgacg ccaactgtga cggggtagtc gatatagtgg atatcattta cctcttaaat
     1381 tacatcacct tcggtgaacc cgtcccctgc ccgttttagc ctcccggaag ttccctaaat
     1441 ttttatctca tttaaatact gcgccgcctc ttccggcgag gtggggttga tgaaaaagcc
     1501 ggtccctttt tcgaagcccg ccacccgggt caccgtgggc atgatttcga agtgccagtg
     1561 gtactccttc tggtgatctc cgtccaccgg gctggtgtgg aggatgtagt taaacggcgg
     1621 gttggccagg gcccgctcca ggcgcaggag gatgtccctc agcactccgg ccagttccgg
     1681 gaactgcttg gggttcaggc gttcaaaggc ggcgtcatga aatctgggca aaatccaggt
     1741 ctcgaaagga tagcggggag cgaacggctc gagcgccaca aagtgctcgg tctgacccac
     1801 cacccgctcg ccatcggcaa tatcctgccg gatgccgtcg cagaaaatgc agcgctcctt
     1861 gtagtcgtag tagcgcttgg cgccttccaa ttcttctaac acctgctgcg ggacaatggg
     1921 cagggcgatc aattgactgt gggcgtgttc cagactggct ccggccgctt ctccctggtt
     1981 tttgaaaatc atgaggtagc ggaagcgcgg gtcgttcttc aaatcgacca tccgctcccg
     2041 gtaggcccag agcacgtcgg aaacctcctg cggggagagg tgcgccagct ctttggtgtg
     2101 atcgggggtt tcgatgatta cctcgtgggc gcccaccccg ttcatcttgt cgaagatgcc
     2161 gaccccttcc cggttcaggt cgccctcgat ccgcagggcc gggtacttgt tggagatgac
     2221 ccgcaccgtc cagccgggag tattgcgggc cgacttgtcg ggccggaaag cgaggatctc
     2281 cggcggcgtc gccgcctcat tcccgggaca gaaaggacag ttcttgctct cgcttttccg
     2341 cggaacgggg gtgaaatcgg tggggcgtcg gctcctttcg gtggagatga tcacccaccg
     2401 cccgaccacc ggatctttgc gtaattcagg catcagttgc tctcaagcct ttctttagag
     2461 ggcggcacac cggaccgtat cggtaacagc acacgacgcc gattcccggc cggttccgat
     2521 caactcgccg cagggtgccg tgattgcccg gccagagttt attccttttc acttatttat
     2581 cggcgttttc ccgcgcggaa ttgttcgaag agttccagga acagccgctt ctgcgccggg
     2641 ctgaacgcca cctcccgccg tctctgcacc tgctcggcgg tcaaaagggc ctggtcgacc
     2701 tgatactcgt caattttccc cgacgtgccc caggaaaatg ggggaatact cttctgcgca
     2761 aacattcccc cgccgtacag gttggagcag aagccgatgc taatgccggt gttcagaagg
     2821 gtgccgatgc cggtgcggac gtgatctccg atgaatgaac cgaccttgag caagccggtg
     2881 tctacttccc ggcccgcaac ctccacgcga acggttccat aattgttctt caggtcacta
     2941 ttcgtggtca gcgcaccgag atttacccac tggcccacgt aggcgtgacc caggaaaccg
     3001 tcgtggtact tgttgtcatg ccctaagaaa atcgactgct ccacttcccc gccgatccgg
     3061 cagaccgggc cgacagacga gccggagcgg atccgaccgc cgaccaaaat tgacctgggc
     3121 ccgatgtagg cgggcccttc aactctggtc tgggcctgga tagtgacctg ccggtcgatg
     3181 aaaatcgggc cggagcgtgc gtcgagcacc gtgccggcat cgattttcgc tccctcgccg
     3241 aggtagacct gctcgacctt gtggatcacg gtctgctgat caacttcgca ccggtcgaac
     3301 atttttttga aatccaaggc cggcgctaag gccgcaaagt cgcgggcgat ctcgtctccg
     3361 ttcgccgcga ctaattccca gaggtaattg gtccagcgcc ctttgaaagg tttccgcggc
     3421 agccggtcta ctaattgccg ctgcgccc
//
LOCUS       DBNGZA010000406         1304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2837993, whole genome shotgun sequence.
ACCESSION   DBNGZA010000406 DBNGZA010000000
VERSION     DBNGZA010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2837993"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1304)
                     /locus_tag="AC16FU_08770"
     CDS             complement(<1..>1304)
                     /locus_tag="AC16FU_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR2994498.1"
                     /translation="RLFLIDGSALAYRAYFAFIRRPLINSKGENTSAAYGFVNSLLKL
                     LREEEPQYVALVFDTKAPTFRHHMYKEYKSTRAKMPDEMVVQLPRIRALAEALNLPIL
                     EQEGYEADDLIGTLAVQAQKAGLTAVMVTGDKDFMQLVDDHAVILNPRQTTEELELLD
                     RVGVKEKIGVPPEKVVDLLGLMGDTSDNVPGIPGVGPKTAVELIEEFGSLEGVLKNAE
                     KIKKEKLRENILQHKEDALLSKKLVTIDTNAPVKLDLKHLRRQPPDEAALRKLFLDLE
                     FTSLLKILGGEGDGKKVVYRLVDDLSGLKSLVAVLKRAGEFAVDTETTNLEPLTCELV
                     GISISKKAGEAFYIPVGHTGPDAKKNLRLDETLALFRPLLESADLKKIGQNVKYDYQV
                     FRNHAIEMKGIACDTMLVSYLLNPTGRQHNLDALAQEHFGHK"
BASE COUNT          267 a          365 c          355 g          317 t
ORIGIN      
        1 atcttatggc cgaagtgttc ctgagccagt gcgtccagat tgtgctggcg gcccgtgggg
       61 ttcaacaggt aggataccag catcgtatcg caagcaatcc ccttcatctc aatagcgtgg
      121 ttccggaaaa cctggtaatc gtacttgacg ttctgtccga ttttttttaa gtcagccgat
      181 tccaggagcg gacggaaaag ggccagcgtc tcgtccaaac gcagattttt cttcgcatcg
      241 ggccctgtgt gtccgacggg aatgtagaag gcctctccgg ctttcttgga gatcgagatg
      301 ccgaccagct cgcaggtgag cggctccaga ttcgtcgttt cggtatcgac ggcaaactct
      361 ccggctctct tgagcaccgc aaccagactt ttgagtcccg agaggtcgtc caccagccgg
      421 taaacaacct ttttcccatc cccttccccg cccagaattt tcaagagcga ggtgaattca
      481 agatcaagga agagttttcg cagcgcggcc tcatccggcg gctggcgccg gagatgtttt
      541 aggtcgagtt tgacaggggc gttggtatca atggtcacta atttctttga aagcaacgca
      601 tcttctttat gctggagaat attctcccgc agtttttcct ttttgatttt ctccgcattc
      661 ttcaatacac cttccagcga accaaactct tctatgagct cgaccgcggt cttgggaccc
      721 acacccggaa tgccggggac gttgtcggag gtgtcaccca tcaagcccag caggtcgacg
      781 accttctccg gcggcacgcc gattttctct ttgactccca cgcgatccag aagttcaagt
      841 tcctcggtgg tctgccgggg attgaggatc accgcgtggt catctactaa ctgcatgaaa
      901 tccttgtcgc cagtgaccat gacggcggtg agtcccgctt tctgcgcctg caccgccagg
      961 gtgccgatca gatcgtccgc ttcgtagccc tcctgttcga ggatggggag attgagcgct
     1021 tcggccaaag cccggatgcg cggcaactgg accaccattt cgtccggcat cttggcccga
     1081 gttgacttgt actccttata catatgatga cggaacgtgg gcgctttggt gtcgaagact
     1141 aacgcgacgt actggggctc ttcttcgcgg agcagtttca aaagcgaatt tacaaagccg
     1201 taggccgccg acgtattctc ccctttggaa ttgatgaggg gccgccggat gaaggcgaag
     1261 taggcgcggt acgctaaagc cgagccgtca atcaaaaaga ggcg
//
LOCUS       DBNGZA010000407         3761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2941553, whole genome shotgun sequence.
ACCESSION   DBNGZA010000407 DBNGZA010000000
VERSION     DBNGZA010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2941553"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="AC16FU_08775"
     CDS             <1..1404
                     /locus_tag="AC16FU_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR2994499.1"
                     /translation="NNIAAGFEDFWTSALREGVFDTVSREEARRAPRPFNTRALSSVR
                     VSKTSAGDFRLVLYPPAMQYDGRTNNNTWLLEMPDPVSKICWDNYAAVAPQTARQLGL
                     AESEVVTLTANGSSLDLPVHLQPGVHPQVLAVAVGWGREKVGRYGNKVGVNAFPLAAL
                     KENALHFSAIPVAVKKTGKKQSLACVQGHHYMESRPIIYAAALEEYRKNPTAGREAEE
                     KLPSIWKAHEYTGYRWGMAIDLNSCIGCNACMVACQAENNVAVVGKAEVLKGREMHWI
                     RIDRYFSGHPDHPEVVHQPMLCQHCEQAPCETVCPTLATVHNEEGLNLQIYNRCVGTR
                     YCSNNCPYKVRRFNWYENFWNIPTPLELVLNPDVTVREKGVMEKCTFCIQRIREGKEK
                     AKEKGIAVADGDIQTACQQTCPTRAIVFGDINDPDSAVSKMSRDPRGYHVLEILNTKP
                     SIAYLTKIRNRRGNGAA"
     gene            1401..2822
                     /gene="nrfD"
                     /locus_tag="AC16FU_08780"
     CDS             1401..2822
                     /gene="nrfD"
                     /locus_tag="AC16FU_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR2994500.1"
                     /translation="MTPVVGQLPLSPAPLAPQREPLVRADIALSDVTRDVLAPLDRKP
                     GPLWLIGFLLAITGVAIGTYVVYNELKIGLGIFGWTHPVMWGVFITYFVFWIGIGHAG
                     TLISAILYLLRQRWRTSINRSAEAMTIFAVICAALFPVLHIGRSWFAYWVIPYPNTRH
                     LWVNFRSPLVWDVFAISTYFTVSLMFWFQGLVPDFATIRDRTTSALKKKIYGLLSLGW
                     AGSVRDWGHYEKAYLLFAGLATPLVLSVHSIVSFDFAVSLMPGWHTTIFPPYFVAGAI
                     FSGFAMVMVLTVVMRKAYHLEEYITLNHLELMNKIIVVTSLMVGYAYLTEAFMAWYSE
                     NPFERHMLANRATGHYAWCYWTMVLCNVLIPQALWFKKVRRNIPLMFAISFLINIPGM
                     WLERFTIIVTSLQHDFLPTTWGNYTPTAFDWGITLGSFGLFFTLFLLFCRFFPTISLA
                     EVKSVLLRPAGRKSRPPAEAAHG"
     gene            2815..3336
                     /locus_tag="AC16FU_08785"
     CDS             2815..3336
                     /locus_tag="AC16FU_08785"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR2994501.1"
                     /translation="MAKVKLPVAVGFFNDPQDLLEAARQAKERGFANLDAYTPFNVHG
                     LDRALGLKPSWVPFATLVAGLSGAALGLYFMYWTSTVNWPLIVGGKPFFSWPAFVPIT
                     FECGILLGGLTTLAALFKACGIPTRKPTLFDPHLTDDRLALVIPLSQPAVHEDPLAFL
                     KGMGAADVRRIVA"
     gene            3314..>3761
                     /locus_tag="AC16FU_08790"
     CDS             3314..>3761
                     /locus_tag="AC16FU_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR2994502.1"
                     /translation="MSGALWRKFAAGAVLFLCAACSRTKSKPPFEYMPDMADQPSVKA
                     QGYDPRAPFYRGMRTPVPGTVPVDFEPYHFATVDTVGPASLINPLPRTLAVLETGRKY
                     YDNFCIVCHGPKGDGQGYIVPKFPSPPSLLTPRVREFPDGRIYHIIT"
BASE COUNT          721 a         1157 c         1053 g          830 t
ORIGIN      
        1 aataatatcg ccgccggatt tgaagatttt tggacttcgg ctctgcgcga aggggtcttt
       61 gacacggtga gccgggaaga agcgagacgc gctccgcgcc cgtttaatac ccgtgcgttg
      121 tccagcgtcc gggtatcgaa aacgtcggca ggcgacttcc ggctcgtcct ctacccaccg
      181 gcgatgcagt acgacggccg gaccaacaac aacacctggc ttctggaaat gcccgatccg
      241 gtctcgaaaa tttgctggga taattacgcc gccgtggcgc cgcagaccgc caggcaactg
      301 ggattggccg aatcggaggt tgtaacgctg accgccaatg gctcaagtct cgatctgccg
      361 gtgcaccttc agcccggcgt ccatcctcag gttttggcgg tggccgtggg ctggggccgc
      421 gaaaaagttg gacgctatgg aaacaaagtg ggggtcaacg cctttcccct ggcggcatta
      481 aaagaaaatg cgctgcactt ttccgcaatt ccggtggcgg taaagaaaac cgggaaaaaa
      541 cagtctctgg cctgcgtgca ggggcaccac tacatggaaa gccggcccat tatttacgca
      601 gcggcgctgg aggagtaccg gaaaaatccg accgccggcc gcgaggccga ggaaaaactg
      661 ccttcgatct ggaaggcgca cgagtatacc ggctaccgct ggggaatggc gattgacctg
      721 aactcctgca tcggctgcaa cgcctgcatg gtggcctgtc aggcggaaaa taacgtggcg
      781 gtggtgggca aagcagaggt gctcaagggg cgcgagatgc actggatccg gatcgaccgt
      841 tacttctccg gccatccgga ccacccggaa gtggtgcacc agcccatgct ctgccagcac
      901 tgtgaacagg cgccctgcga gaccgtttgc cctacgctgg cgaccgtgca taacgaagaa
      961 ggtttgaacc tccagattta caaccgctgc gtcggcaccc gctactgctc caacaactgc
     1021 ccctacaaag tgcgccgctt caactggtat gagaatttct ggaacatccc caccccgctg
     1081 gagttggtct tgaacccgga cgtgacggtg cgcgaaaaag gggtcatgga aaagtgcact
     1141 ttttgcatac aaaggattcg ggaagggaag gaaaaggcaa aagaaaaagg tattgcggtg
     1201 gccgacggtg acatccagac cgcctgccag caaacctgcc cgacccgggc catcgttttc
     1261 ggcgacatca atgatcccga cagtgccgtg tcgaaaatgt cccgcgatcc gcgcgggtac
     1321 cacgtgctgg agatcttgaa caccaaacct tccatcgcct acctgacgaa aatccgcaac
     1381 cgccggggga acggagccgc atgacgccgg tagtcggaca actgccgctc tctcctgctc
     1441 cgctggcacc tcagcgagag cccttagtcc gcgccgacat cgccttaagc gacgtgaccc
     1501 gtgacgtgct ggcgccttta gaccgtaaac ccggtccgct ctggctcatt ggttttctcc
     1561 tggccatcac cggagtcgcc attggcacct acgttgtata caacgagctc aaaatcgggc
     1621 tgggaatttt cggctggacc cacccggtga tgtggggcgt gttcatcacc tatttcgtct
     1681 tctggatcgg gatcggccac gccggaacct tgatttccgc cattctgtac ctcttgcggc
     1741 aacgctggcg cacctcgatc aaccgctcgg cggaggcgat gacgattttt gcggtgattt
     1801 gtgcggcgct ctttccggtc ttgcacatcg gccgcagctg gttcgcctat tgggtgatcc
     1861 cgtatcccaa cacccgccat ctctgggtca acttccgctc ccccttagtg tgggacgttt
     1921 ttgccatttc cacctacttc accgtctccc tcatgttctg gttccaggga ctggtccccg
     1981 acttcgccac catccgggac cggaccacaa gcgccctgaa gaaaaaaatt tacggcctcc
     2041 tctctttggg ctgggccggt tcggtgcgcg actggggcca ctacgagaag gcctacttgc
     2101 tcttcgccgg cctggccacg cctctggtgc tctcggtgca cagcattgtg tcgtttgatt
     2161 tcgccgtctc gctcatgccc ggctggcaca ccaccatctt ccccccgtac tttgtggccg
     2221 gcgcgatttt ctccggcttt gccatggtga tggtcttgac ggtagtcatg cgcaaagcct
     2281 accacttgga agagtacata accttgaacc atctggagtt gatgaataaa atcatcgtgg
     2341 tgacctccct catggtgggg tacgcgtacc tcaccgaagc cttcatggcc tggtacagtg
     2401 aaaatccatt cgagcggcac atgctggcca accgggccac cggccattac gcctggtgtt
     2461 actggacgat ggtcctctgt aacgtcctca tccctcaggc gctctggttc aagaaagtca
     2521 ggcgcaacat cccgctcatg ttcgccattt cttttctgat caatatcccg ggaatgtggc
     2581 tggagcgttt caccatcatc gtcacctctc ttcagcacga ctttttgccg actacctggg
     2641 ggaactatac gccgacggct ttcgattggg gtatcacgct cggttctttc ggtctcttct
     2701 tcactctttt cctgcttttc tgccgcttct tccccaccat ttccctggcc gaagtgaaaa
     2761 gcgtgctcct gcggcccgcc ggaagaaagt cgcgtccgcc tgcggaggca gcgcatggct
     2821 aaggtaaaat tgccggtggc ggtcggtttc tttaacgacc cgcaggacct gctggaagcc
     2881 gcccgccagg cgaaagagcg cggctttgct aacctcgatg cctacacccc cttcaacgta
     2941 catggtctgg atcgggcgtt gggtctgaaa ccttcctggg tgccgtttgc cacgctcgtg
     3001 gcggggctct ccggtgccgc gctgggactc tactttatgt actggacttc gacggtgaac
     3061 tggcccttga tcgtcggcgg caaaccgttt ttttcctggc cggccttcgt gccgattacc
     3121 tttgaatgcg gaattctcct gggcggactg acgaccttgg ccgccctgtt caaagcttgc
     3181 ggtattccga cccgcaagcc aacgcttttc gatcctcacc tgaccgatga ccggctggct
     3241 ttagtgattc ctctgtctca gccggcggtg catgaagacc cgctggcctt tttgaaagga
     3301 atgggcgctg ccgatgtccg gcgcattgtg gcgtaaattt gcggcgggag ccgtcctgtt
     3361 cctgtgcgct gcttgttcac ggaccaaaag caagccgccc tttgaatata tgcccgacat
     3421 ggccgaccag ccgtcggtga aggcacaggg atacgacccg cgagcgccgt tctaccgcgg
     3481 gatgcgcacg cccgtaccgg gaaccgtgcc ggttgatttt gaaccgtacc acttcgccac
     3541 tgtcgatacg gtcgggccgg cgagtttgat caatcccctt ccgagaactt tggctgtcct
     3601 ggagaccgga aggaagtact acgacaactt ctgcatcgtc tgccacggtc ccaaaggcga
     3661 cggtcagggc tacatcgtgc cgaaatttcc ctcccccccg tcgcttttga ccccacgggt
     3721 gcgggagttt cccgacggcc ggatttatca cattatcacc c
//
LOCUS       DBNGZA010000408        10425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2942406, whole genome shotgun sequence.
ACCESSION   DBNGZA010000408 DBNGZA010000000
VERSION     DBNGZA010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2942406"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="AC16FU_08795"
     CDS             <1..1895
                     /locus_tag="AC16FU_08795"
                     /inference="COORDINATES: protein motif:HMM:NF052691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR2994503.1"
                     /translation="STDGGLTWVLVQATAFSIRSLAHDPTTGALYAATFGSGLYKSTD
                     LGQNWSPILVKDFITNDQLVHQRAVAVFGRDSLYVGGSITDIDATGGSLFKSTDGGVS
                     WTQVQPGLAIRSSTMSIAISPNQPDSSLIIGTAAKGVYKSINGGLNWLAINGAGTANP
                     LPARPAPPDSGHLQITAVAFSPSYRYAVVESTGLVYRRTLGDTTGGVGWLPATGLPGM
                     NAGIQALTGFSQSLARFLSGSEGDGAFRSGDSGKTWAAENTGLLGVAAAALLFSQNHT
                     LLLGTDFGDQVWRSTDSAGSWVLADSLDNFNSVRDLEQAHVDGAPIYAALYGTGVYKS
                     TDDGRHWLLTDTATLGNHLVRPLAVVPNKPDTIFAGTGNGVYKTSNGGATWLAASSGI
                     PLSTSIRSLALDPANPNLLLAGTDLDYLYRSTDGGTSWSHITNANGLATGLPFIRSLA
                     FDPAAPAVVYAGGDSGRIFRSMDAGATWSTRAVLTTPFSVRSIVIDPHDRSILFAATF
                     GSGVFLSTDTGGSWNQFNSGLSDLEILTVIADTATPLGLYAGTANSGVFATKFTPPPP
                     GNKGDVTGDGVVDILDVVSIIQYVVFGAPLPVPAGADVNCDTVVDIIDIVAVIQFVVF
                     GSPFPC"
     gene            1933..3105
                     /gene="pgsB"
                     /locus_tag="AC16FU_08800"
     CDS             1933..3105
                     /gene="pgsB"
                     /locus_tag="AC16FU_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HIR2994504.1"
                     /translation="MWVVISLVILLSAYGFWEFRQHQKVVRTIPLRIHVNGTRGKSSV
                     TRLIAGGLRAGGLVTFAKTTGSRPRLIFPDGSETRVVRAGKANIIEQVAILRRAAACQ
                     AQVLVMECMAVQPVLQRLVEQQMIKSTAGVITNVRADHLDEMGPTLEDVAKALGGTIP
                     KNGFLFTSEKNFLPLFQAIAQRVGTTVVGIDSEGVTAEMLSGFSYLEHPENVALALAV
                     CNHLGVPAPTALAGMQTAQPDAGVLRIYRIQQGERTVDFVNAFAANDPDSYHIIRERL
                     ERILNPQARLVLLVNSRADRIQRAHQLGDLVAEWPAFRVVVTGEYTTALVNRAIRSGL
                     APQRIENYGGHTVEDIYEHLVRMADHHAAVVGIGNIVGFGEQIVNFFVNRGTEFAY"
     gene            3125..3586
                     /gene="pgsC"
                     /locus_tag="AC16FU_08805"
     CDS             3125..3586
                     /gene="pgsC"
                     /locus_tag="AC16FU_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HIR2994505.1"
                     /translation="MQSIGLGLVVSLVFSETLGLAAGGMVVPGYAALMIHHPLRIVGT
                     ILASLATFGTLKYLSNYIFIYGRRRTVWVILVGFCFGWLSRELLVLNIGPIALEMQIF
                     GLVIPGLIANWMERQGVVQTITTLVIAAVLVRLLLMVISGGDVQLGADVIL"
     gene            3583..4722
                     /gene="pgsW"
                     /locus_tag="AC16FU_08810"
     CDS             3583..4722
                     /gene="pgsW"
                     /locus_tag="AC16FU_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HIR2994506.1"
                     /translation="MIWRQGKVSAFTLVGLALFAILIFVLAERTAQPERQPAFTTKLK
                     AAKQALVAQQTIKEYSQTLGTAIDRENDPYVTGLIGQERTPITSDRGVVTSKILATNP
                     NFAAAFVNLLLKAKVKKNDVVACGMTGSLPGWNVAFYAACWAIGARPLVITSVGSSDW
                     GANNPALTWLDMERVLADRGIFPFRSVDASIGGGSDNGRGMSPEGRQLIREAVKRNAV
                     ELIEAPDLEANIGKRMEIFDRRAENRKIVTYVNIGGGLASLGGSQNERLIPAGLSLHL
                     AAKNFPVRGVINRMAERGLPVIHLLEVEKIAERYGLPAVVLPEPPELGEGPLFFKDTF
                     SVTSTSILTALLLMVLFVFIRLDVSHYLFRRKRGTAQPAPVGTGK"
     gene            4871..5776
                     /locus_tag="AC16FU_08815"
     CDS             4871..5776
                     /locus_tag="AC16FU_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994507.1"
                     /translation="MRRRILFWFFVGVGLFATAVAGGAKKVRSVSITPVTGRPVAVVI
                     DEEVTSYFLCSAKEPLEISVSGPGTLEIATRLLFPKGKTEGKYTVELREGGKVLKRHE
                     TQTQVAAAKLSGRSEVPGKSRKFRYDLPEGQHRLTLAFVTGEYPEVAARFTFSVRKNK
                     GEFVAITPLSFARTATALIKEKQFTYYVATPTKPVQFRVVGPVRVQIVSRLTYDERLK
                     GTQKYTVTATEGKKLFNQFALETTKSTSVVYNDWPEVAPGKSRTFYIDVPAGEHLYDF
                     ALTGSVARAVALRFSLPHTALSNEP"
     gene            5864..7036
                     /locus_tag="AC16FU_08820"
     CDS             5864..7036
                     /locus_tag="AC16FU_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994508.1"
                     /translation="MRAKGSWWLAWCFGTTLVTLNAVAQTPPAPPAGSADSVVATQAV
                     PPGTLPIAQKRTGRRTPAPFFSGKLTLGTTYDNNILDYSARDVRLLDSGTQPTRFAIR
                     SAGDFIFGAGTQFDFRPVIWENRPTRLRLSSAGDLYARDGVKNKGEFGAELRQSLFRH
                     AALLTGIDYLPSFYLRNLFWRGHPLGSPAYARARFRRWTYSGAFAYDFSSRVDVQVGY
                     AFETRNYNREFNERDSKSHVVNGEIDYDLAKRLRVDLSYEFDWTLADGRHNPDPRVVD
                     ISNRAHQFAMDFFFRPVVWRHHPFQLVQRAAYQFQDYTSNRPLDRYHFGRKDHEWHLR
                     TAVSLDLTRSLEAGLAYTFAMATTNTSVTAPPPAVSLADVGDYKSHALAARVTVSF"
     gene            complement(7605..8627)
                     /locus_tag="AC16FU_08825"
     CDS             complement(7605..8627)
                     /locus_tag="AC16FU_08825"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR2994509.1"
                     /translation="MKAAVFYEAHHPLRVEEVPLPRIKPDEILVKVAACGVCHTDLHY
                     LDHGVPTFKKPPVILGHEPSGTVAEIGVEVGNFKTGDRVLLPAVLTCGRCDYCRTGRE
                     NICSAQAMLGNNVDGAYAEYVKAPAKDAFHLPNEIPLIEGSIIADAISTPFHAVKNRA
                     RVQPGDTVVVFGCGGIGINVIQVAKAAGGIVLAVDIREKKLDWAKQFGADYVVLAQET
                     EDAAKQIRKLTGGGADVAIEAIGNPLTIQQAFSSLRTGGRLVLVGYSPEDVSLSAARI
                     MYREMEIMGSLGCRPVDYPKIIALVKLGRLKVKELVTHKLPLKEIHAAFELMRRGESL
                     RSVITF"
     gene            8845..9501
                     /locus_tag="AC16FU_08830"
     CDS             8845..9501
                     /locus_tag="AC16FU_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR2994510.1"
                     /translation="MTSSIHRAAGKRSPRSERLARLLSLAAHLIAQEGYQKASIRKVA
                     RAANVSIPSLYHYFKEKEEMLFLIQYHTFDSLVRQLKERLPPATSPEQKLYRMIENHL
                     EHFLTSMDELKVCAKELQSLSGRYYGKVLQKRQEYYQLTVEIVEELKEKFRNTKVDSR
                     LATLFLFGMLNWIHMWYRPGDGDSLKKITEQMHNLFLFGLGEIPMSRRAGRRKENRDV
                     "
     gene            9494..>10425
                     /locus_tag="AC16FU_08835"
     CDS             9494..>10425
                     /locus_tag="AC16FU_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR2994511.1"
                     /translation="MFEVFRNWEKGRHAYAKEWKQRTGGKVLGYFCTYVPEEILYAAG
                     VLPVRILGSHEPCDLTEPHIFGMFCPFSRDCLAQGLKGRYDYLDGIMIAQSCLHIRQA
                     FTSWQKHLPVDFSYYLCMPHNLQSVRAESYLTGELLEFKKAVEKWTGKEIGAECLSRA
                     IETYNCSRRLLNQVYATRKEEHPPLTGEEAMEMVISNQVTDKSEHNRALEELAQNLPN
                     RKIDRQTGTRLMLIGSENDDIPFIQMLEALGATVVIDEHCTGTRYFWNEVTPRENLLE
                     AIAQRYLQRPPCPTKDWVRRRVEHTLDLAKAYRV"
BASE COUNT         2181 a         2993 c         3048 g         2203 t
ORIGIN      
        1 ggagcaccga cgggggactc acctgggtct tggtgcaggc gacggccttt tcgatacgct
       61 ctctggcgca cgatccgacc accggcgcac tctacgccgc cactttcggg agtgggttgt
      121 ataagagtac tgatttaggc cagaattggt cacccatttt ggtcaaagat ttcatcacca
      181 acgatcaact tgtccaccag cgggcggtgg cggtttttgg gcgcgattcc ctctacgtgg
      241 gtggatcgat caccgatatt gacgccactg gcggctcgct cttcaaaagc accgacgggg
      301 gagtgtcctg gacccaggtg cagcccggac tggctatccg cagcagtacg atgtcgatag
      361 ctattagccc caaccaaccc gattcaagtt tgatcatcgg gacggcagcc aaaggggtct
      421 acaagagcat caacggcggg ctcaactggc tggcaataaa tggtgccggg actgcgaacc
      481 cgctgccggc gcgtccggca ccacccgatt ccggacactt gcaaataact gcggtagcgt
      541 tcagtccctc ctaccgttat gccgtggtgg aatcgaccgg cctggtctac cgtcggacct
      601 tgggcgacac cacaggtggc gtcggctggc ttccggcaac gggtttgccc ggaatgaacg
      661 ccggtatcca ggcgctgacc ggattcagtc aaagcttggc ccggtttctt tctgggtccg
      721 aaggcgatgg ggctttccgc agcggtgatt cgggcaaaac ctgggccgcg gagaatacgg
      781 gacttttggg cgttgctgcc gcggcgctcc tcttttccca gaatcacacc ctgctcttag
      841 gcactgactt cggcgatcaa gtctggcgct cgaccgattc ggctggcagt tgggttctgg
      901 ccgactcgct ggacaacttc aactcggtgc gcgacctgga gcaggcgcac gtagacgggg
      961 cgcctatata cgcggctctg tacggcactg gcgtctacaa gagtaccgac gatggacggc
     1021 actggctact gaccgatact gctaccctgg gtaatcatct tgtgaggcct ttggcggtgg
     1081 tgccgaataa accggatacc atcttcgcgg gcacggggaa cggcgtttac aaaacgagca
     1141 atggcggcgc tacatggctg gcggcaagta gcggtatccc cctttccact tcaatccggt
     1201 cactggcgtt ggatccggcg aacccaaacc tcctgctggc gggcactgac ttggattatc
     1261 tctatcgttc aaccgacggc ggtacgagtt ggtcacacat caccaatgcg aacgggctgg
     1321 ctaccggttt gccgtttatc cgcagtctgg ctttcgatcc cgccgccccg gcggtcgttt
     1381 acgcgggggg tgattcgggg aggatttttc gcagtatgga tgccggggcg acctggtcga
     1441 cgcgggctgt gctcaccacc ccgttctcgg tgcgctcgat cgtgatcgat ccccacgacc
     1501 gcagcattct ctttgccgcc accttcggtt ccggagtttt cctcagcacc gacaccggcg
     1561 gaagctggaa tcagttcaac tcaggtctga gcgatttgga gattttgacg gtcattgccg
     1621 acacggcgac gcctttgggt ctctacgccg gcacggcgaa ctcgggagtc ttcgccacca
     1681 aatttacccc cccacccccg gggaataagg gggatgtcac cggcgatggc gtggtcgata
     1741 tccttgatgt ggtgtcgata attcagtatg tcgtgtttgg ggctccgctg ccggttccag
     1801 ccggcgccga tgtcaattgc gacacggtag tggacatcat tgatattgtc gcagtgatcc
     1861 aattcgtcgt gttcgggagc ccgtttccat gttagcccag cggccgaatc tctctctggg
     1921 aatagaacag agatgtgggt agtcattagt ctcgtcattc ttctttcggc ctacggtttc
     1981 tgggagtttc ggcaacacca gaaagtcgtg cgtaccattc ccttgcgcat ccacgtcaat
     2041 ggaacgcggg ggaaatcctc ggtgacccgg ctcattgcag gggggttgcg ggcgggcggc
     2101 ctagtcacgt ttgccaagac taccggcagc cgcccacggc ttatcttccc ggatggctcc
     2161 gagacgcgag tggtgcgcgc gggcaaagcg aacattatcg agcaggtagc catcctaagg
     2221 cgggcggcgg cctgccaggc gcaggtcctg gtgatggagt gcatggcggt gcaaccggtt
     2281 ttgcagcgat tggtggaaca gcaaatgatc aaatcgacgg ccggggtcat taccaacgtc
     2341 cgcgccgacc atttagatga gatgggacca acgctggagg acgtcgcgaa ggcattgggg
     2401 ggaacgattc cgaaaaacgg gttcctcttc acctccgaga aaaactttct cccgttgttc
     2461 caagccatcg cgcagagagt gggcactacc gttgtgggga tcgatagcga gggtgtcacc
     2521 gcggagatgc tctctggttt ttcctacctg gaacacccgg agaacgtggc tttggccttg
     2581 gcggtctgca accacctggg tgtcccggcg ccgacggcct tggccgggat gcagacggcg
     2641 cagccggacg ccggcgtgtt gcggatctac cggatccagc agggggagcg gacggtcgat
     2701 ttcgtcaacg cttttgcggc caacgacccc gattcctacc acatcatccg ggagcgcctg
     2761 gagcggattc tcaatccgca ggcgcggctc gtcctcctgg tgaactcccg ggccgaccgc
     2821 attcaacggg ctcaccagtt gggcgacctg gttgcggagt ggccggcctt ccgcgtggtg
     2881 gtcaccggcg aatacaccac cgcgctggtc aaccgggcca ttcgttccgg cctggcgccg
     2941 cagcggattg aaaactacgg cggccatacg gtggaggaca tttacgagca cctggtgcgc
     3001 atggccgacc accacgccgc ggtagtgggg attggaaaca tcgtcgggtt cggcgaacag
     3061 atcgtgaact tcttcgtcaa ccgggggacc gaatttgctt actgaagcgg cgcgcctttt
     3121 gatcatgcag tcgatcggac tggggctggt ggtgagcctg gttttctccg aaaccctggg
     3181 gttggccgcg ggcgggatgg tggtgcccgg ctacgcggcg ctcatgatcc accacccttt
     3241 gcgcatcgtg gggacgattc tggccagtct ggccaccttc ggaacgctca agtacctttc
     3301 caactacatc ttcatctacg gccggcggcg caccgtctgg gtcatcctgg tgggcttctg
     3361 cttcggctgg ctctcgcggg aactgctcgt tctaaacatt ggcccgattg ctttggaaat
     3421 gcagattttc ggtctggtca tccccggact cattgccaac tggatggaac ggcagggggt
     3481 cgtgcagacg atcaccaccc tggtcattgc cgcggtcctg gtgcgcctgc tcttaatggt
     3541 catctccggc ggcgacgtgc agttgggggc ggacgtgatt ttatgatctg gcgtcagggg
     3601 aaagtttctg catttacgct cgtcggactg gcactctttg cgattctgat tttcgtgttg
     3661 gcggaacgga cggcccaacc cgagcgccag cccgctttca caaccaaact gaaggcggcc
     3721 aagcaggcgc tggtggctca gcaaacgata aaggaataca gccaaactct cggtaccgcc
     3781 atcgaccgag aaaatgatcc ctatgtcacg ggcttgatcg gccaggaacg gacgccgatc
     3841 acctcggatc gcggggtggt cacgtcgaaa attttggcca ccaacccgaa ttttgccgcg
     3901 gcgttcgtga acctgctcct caaggcgaaa gtcaagaaaa atgacgtcgt cgcctgcggc
     3961 atgaccggat cactgcccgg ctggaacgtc gctttctacg ccgcctgctg ggctatcggg
     4021 gcccggccgc tcgtcatcac ctccgtcggt tcttccgact ggggtgccaa caatccggct
     4081 ttgacctggc tggatatgga gcgggttttg gccgaccggg gaatttttcc cttccgctcc
     4141 gtggacgctt ccatcggcgg cggctccgac aacggccggg ggatgtcgcc ggaggggcgg
     4201 caattgatcc gcgaggcggt caagcgcaat gccgtcgaat tgattgaagc gcccgacctg
     4261 gaagccaaca tcggcaaaag gatggaaatt tttgaccgga gggcggagaa ccggaaaatc
     4321 gtcacgtacg tcaatatcgg cggcggactg gccagtctgg gagggagcca gaacgagcgg
     4381 ctcataccgg ccggtttgtc cctgcacctg gcagcgaaaa atttcccggt gcgcggcgtg
     4441 atcaaccgga tggcggagcg gggcctgccc gtgattcacc tcctcgaagt cgaaaaaata
     4501 gcggagcgct acggcctgcc ggcggtggtg ctccccgaac ccccggagct gggtgaaggg
     4561 ccactcttct tcaaagacac cttttcggtc acctcaactt cgattcttac ggcgttgttg
     4621 ttgatggtcc ttttcgtttt catccgactc gacgtctcgc actacctgtt ccggcgaaaa
     4681 cggggaacgg cgcagccggc gccggtcgga accggcaaat gagaaagaca gaatagagga
     4741 gtgaccgccc cggcagtgaa gggaaattac tatcaggaca ccgtagacag gacgcagtca
     4801 acggagaaga gaaaaaattc aggatcaaat cggagataga ggccttaaac ctgcaaagaa
     4861 aaggatgact atgcgccgaa gaattctctt ctggtttttc gtcggagtgg ggcttttcgc
     4921 caccgccgtt gccggtgggg ccaagaaggt ccggtcggtg agcatcaccc cggttaccgg
     4981 gagaccggtg gcggtcgtga tcgacgaaga ggttacctcc tacttcctct gttcggcaaa
     5041 agagcctttg gaaatttcgg tgagcggacc gggaacactg gaaatagcca cgcggctgct
     5101 tttccccaaa ggcaaaaccg aagggaagta caccgtggaa ctccgcgaag ggggtaaggt
     5161 cctcaaacgg cacgagaccc agactcaggt ggcggcggcc aagctctccg gtcgttccga
     5221 agttcccggc aagagccgca agttccgcta cgacctgccg gaaggtcagc accggctgac
     5281 gttggcattc gtgaccggag aataccctga agtggcggcg cgcttcacct tctcagttcg
     5341 gaaaaataag ggcgagttcg tagcgatcac gccgctttca tttgcccgca cggccaccgc
     5401 actcatcaag gaaaagcagt tcacctacta cgtggcgacg ccgactaagc ccgtgcagtt
     5461 ccgggtggtg gggccggtcc gggtgcagat agtgagccgg ctgacctacg atgagcgcct
     5521 gaaagggacg cagaagtaca cggttaccgc aaccgaaggc aagaagcttt tcaatcaatt
     5581 tgctctggaa accaccaaat cgacttcggt ggtttacaac gactggccgg aggtggcgcc
     5641 gggcaagagc cggacgtttt atattgacgt ccccgccggt gaacacctct acgattttgc
     5701 actgacgggc agtgtggcca gggcggtggc gttgcggttt tcgctgccgc acacggcgct
     5761 ttccaatgaa ccgtaagtgt gactgtatcg ctcaagaaat ggtgaaaggg cgaccaggtc
     5821 acgcacctga ggagaccggg accggtcgag aagagaggag cgcatgagag ctaaaggtag
     5881 ctggtggctc gcctggtgct tcggaacgac gctggtgacg ctgaacgcag tggcgcaaac
     5941 tccccctgcg ccgccggccg gttcggctga ttcggtcgtg gcgacgcagg cggtgccgcc
     6001 cggcactttg ccgattgccc aaaaacgcac cggccggcgg accccggccc cctttttctc
     6061 gggcaagttg accttgggca ccacctacga caataacatt ttggattatt cggcccggga
     6121 cgtacggttg ctcgactccg gcacccagcc gacccgcttt gccatcaggt cggccggcga
     6181 tttcatcttc ggggccggta cccagtttga tttccgcccg gtgatctggg agaaccgtcc
     6241 cacccggctg cgtctttcgt cagcgggcga cctatatgcg cgcgatggcg tcaagaacaa
     6301 gggggagttt ggggcggagt tgcggcagtc cctcttccgt cacgcggcgc ttttgaccgg
     6361 gattgactac ctgccctcct tttacctgcg caacctgttc tggcgcggtc acccgctcgg
     6421 aagtcccgct tacgcccggg cacgcttccg gcgctggact tattccgggg cttttgccta
     6481 cgatttctcc tctcgggtcg acgttcaggt cggctacgcc tttgagacgc ggaactataa
     6541 ccgggaattc aacgaacggg acagcaagag ccacgtcgtc aacggggaaa tcgattacga
     6601 tctggccaag cggttacgcg tggatctctc ctatgagttt gactggacgt tggccgacgg
     6661 tcggcacaat cccgatcctc gggtggtcga tatttccaat cgggcgcacc agttcgccat
     6721 ggactttttc ttccggccgg tggtctggcg ccaccacccg tttcagctcg tgcaacgggc
     6781 ggcgtatcaa ttccaagact acacctccaa ccgccccctc gaccgttacc atttcggtcg
     6841 gaaggatcac gagtggcacc tgcggacggc ggtcagtctg gatttgaccc gctccctgga
     6901 agccggtttg gcgtacacct ttgccatggc gacaaccaat acttcagtga cggctccgcc
     6961 cccggcggta tctctggccg atgtcggcga ctacaagagt cacgcgctgg ccgcgcgagt
     7021 gacggtttcc ttttagagcg atatcgaaat ccccaatatt acaggaaaga ttcgagcgca
     7081 gtccgcgtcg gagttagccg aaccagacgg aggagtttct cggtgtcgac cgagagcgcc
     7141 agcgccaagt atcgggtagg tggttaacaa gttcggagag aaccaagaaa cttgactacg
     7201 agaagacttg ccaggtaaca gccaagaaga tgcatttgcg ggaggtgtcg gaaagatatt
     7261 cctttgatac gacaaaatta gcgaaggccg ggcgtaaact gacgatctcg aatttctgta
     7321 gctaccattt gtacctttcc ggtgacactg taccggtcct ccaccctaat tcctcagatg
     7381 tattgtggat taaggacgtg gaagatagaa acgcattgac aattttcagg tttttttacc
     7441 gggactggca cttccagtct cccatctacc taccgtactt tcgtccaccc ttaaacagta
     7501 tcgatcactg gatcggaacc tctggctgtg aaagccgggt ggtaggtgat aaggatcgaa
     7561 ttgctcggcg ttttcattgg tatttgaact cgcgatttat tgactcaaaa ggttatgacc
     7621 gatctcaagg actcgcctct gcgcataagc tcaaaggcag catggatctc cttcaaaggt
     7681 aatttgtgag ttaccaactc tttcaccttc agccggccca gcttgaccaa cgcgattatt
     7741 ttcgggtaat caaccggccg gcatcccaga gaacccataa tctccatctc ccgatacatt
     7801 atgcgcgccg cgctcaggga aacatcttct ggtgaatacc ctaccaacac caatctccct
     7861 ccagttcgaa gggagctgaa cgcctgttga atggtcagcg gatttccgat ggcttcaatg
     7921 gccacatccg caccgcctcc ggtcagtttt ctgatttgct ttgctgcatc ctcagtttct
     7981 tgagcaagga cgacgtagtc ggccccgaat tgttttgccc agtctaattt cttttcccga
     8041 atatccaccg ccaggacaat gcccccggcc gctttggcca cctgaattac attgatgccg
     8101 ataccgccac agccaaagac gaccacggtg tcaccgggct gaacccgggc ccgattcttg
     8161 accgcatgga agggggtcga aatggcatcg gcaatgatgc tgccttcaat taaggggatt
     8221 tcgttcggca aatggaaagc gtctttggcg ggcgccttta catattccgc atacgcgccg
     8281 tccacattgt tccccagcat ggcctgagcg ctgcaaatgt tctcccggcc cgttcgacag
     8341 tagtcgcagc ggccgcaggt caaaacggcg ggcaatagca ctctatctcc agtcttgaaa
     8401 ttgccgactt cgacaccaat ttcggccaca gtgcccgagg gctcgtggcc taagatcacc
     8461 ggaggtttct taaacgtggg aacgccgtgg tccaggtagt gcaggtcggt gtgacagacg
     8521 ccacaggccg ccactttcac cagtatttca tccggtttga ttcgggggag gggaacttcc
     8581 tccacccgca gggggtgatg ggcttcatag aaaaccgccg ccttcacttc gtcctcctct
     8641 ccacaaaaag aacgtgcaag agcaaaaacc gcctcacgag tatatccaga tctccgatga
     8701 ttgccaagca gaaggggggc tttcccgaag accgtcacgc aaatccagac taaactgtca
     8761 gcttttctat tttttccctt gacagccgca gcgcagcgta cgtatatatt atcgaacgtt
     8821 cgatagaata aagagttcac gaccatgacc tcttctattc accgcgctgc gggaaaaaga
     8881 tccccccgct cggaacggct ggcccggctg ctcagcctgg cggcccacct cattgcccag
     8941 gagggctacc agaaagccag catccgcaag gtggcccggg cggcgaacgt cagtatcccc
     9001 agcctgtacc actacttcaa ggaaaaagag gagatgctgt ttttaattca ataccacacc
     9061 ttcgattccc tggtgcggca gctgaaggag agactgccgc cggccacctc cccagaacag
     9121 aaactctacc ggatgattga aaatcacctg gagcatttcc tcacctctat ggatgaattg
     9181 aaggtctgcg cgaaagagct gcaatctttg tccggacggt attacgggaa agttctgcag
     9241 aagcgtcagg agtactatca actgaccgtg gaaatcgttg aggaactcaa agagaaattc
     9301 cgcaatacga aggtggattc tcgcttagcc acactctttc tctttgggat gctgaactgg
     9361 atccacatgt ggtaccgacc gggggacggt gactcgctga aaaaaatcac cgagcagatg
     9421 cataacctct ttctcttcgg gctcggggaa attccaatga gccgccgggc cgggcgccgg
     9481 aaggaaaatc gagatgtttg aggtattccg aaactgggaa aaaggcagac acgcctacgc
     9541 caaagagtgg aaacagagga ccggcgggaa agttttgggc tatttctgca cctacgtccc
     9601 cgaggagatt ctctacgctg ccggggtcct accggtcagg attttaggca gccacgagcc
     9661 gtgcgatttg accgagccgc acattttcgg gatgttctgc cctttttccc gggattgtct
     9721 ggcccagggg ttgaaaggtc gctacgatta tttagacggg atcatgattg cccagtcctg
     9781 cctgcacatt cgtcaggcgt tcaccagctg gcagaagcac ctcccggtcg atttcagcta
     9841 ctatttgtgc atgcctcata atcttcaaag cgtgagagct gaaagctacc tgacgggtga
     9901 acttctcgaa ttcaaaaaag cagttgagaa atggaccggc aaagaaatcg gtgcggaatg
     9961 cctcagccgg gccattgaaa cctacaattg cagtcgccgg ctgttaaacc aggtctacgc
    10021 cacccggaag gaggagcatc cgcccctcac cggggaagag gcgatggaaa tggtcatttc
    10081 caatcaggtg accgacaaat ccgagcacaa ccgggcttta gaagaattgg ctcagaatct
    10141 cccgaacaga aaaatagacc gacagaccgg aacccggttg atgctcatcg gcagtgagaa
    10201 tgacgacatt cccttcatcc agatgctgga agcgctgggg gcgaccgtgg tcatcgatga
    10261 gcactgcacc ggaaccagat acttctggaa tgaagtcact ccccgggaga atttactcga
    10321 agctatagca cagcggtacc tccagcgccc tccctgcccc accaaagatt gggtgcggcg
    10381 gagagtcgaa cacacgctcg atctggcgaa ggcctatcgg gtcca
//
LOCUS       DBNGZA010000409         1645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2942443, whole genome shotgun sequence.
ACCESSION   DBNGZA010000409 DBNGZA010000000
VERSION     DBNGZA010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2942443"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="AC16FU_08840"
     CDS             complement(<1..1128)
                     /locus_tag="AC16FU_08840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2994512.1"
                     /translation="MRYRSLTRAMFGLALFLFLCGTAFGFELPVQKKTLKNGMTVLVL
                     ENHTAPVVSTYLRFKVGSVDEHPGITGTSHLLEHMMFKGTKDIGTANYKAELPVMEKI
                     DQTAHQIIAEQARLQNPLSDHDSTKLKALREQLHTLQAEEKKYVIKDELWETYLKNGG
                     TGLNASTSEDGTQYFVSLPANRLELWAFLESDRIANPILREFYSERDVVYEERRLRTD
                     TQPFGKLSEQFGAAAYIAHPYGWPVVGWASDLETVSREEVEKYFKRYYAPNNAIAAIV
                     GDVKAPEVFALMDKYFAPIPPQPAPDPVFTTEPPQEGERRVKAEFDANPLLLIGWHIP
                     ALGNPDWFALDVLSDILSQGRTSRLYKKIVEEKKLATNISSF"
     gene            complement(1356..>1645)
                     /locus_tag="AC16FU_08845"
     CDS             complement(1356..>1645)
                     /locus_tag="AC16FU_08845"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2994513.1"
                     /translation="VLLLWYYVKEKSQPQSSKPVGYLVIDVLPSADIYVDGKYRGSTP
                     PPLLLNLDPGSHRVHFAGENLNLKERSVALTEGETVLLKERLKPAGNGAGE"
BASE COUNT          316 a          446 c          508 g          375 t
ORIGIN      
        1 gaaagaggag atgttggttg ccagtttctt ttcttccacg atctttttgt agaggcggct
       61 ggtgcgtccc tgggacaaaa tgtcggagag gacgtccaag gcgaaccagt ccggattccc
      121 cagagccgga atgtgccagc cgatcaagag cagggggttg gcgtcgaact ccgccttcac
      181 ccggcgctcg ccttcctggg ggggctcggt ggtaaagacc gggtcgggcg cgggctgggg
      241 cggaatgggg gcgaaatatt tgtccatcag ggcgaatact tcgggggctt tcacgtcgcc
      301 cacgatcgcc gcgatggcgt tgttgggagc gtagtagcgc ttgaagtact tttccacttc
      361 ctcgcgggag acggtttcca agtccgaggc ccagcccacc accggccagc cgtagggatg
      421 cgcgatatag gccgccgcgc cgaactgctc ggataacttc ccgaacggct gcgtgtcggt
      481 ccggagccgc cgttcctcgt agaccacgtc gcgttcggag tagaattccc gcaaaatcgg
      541 gttggcgatc cggtcggatt ccagaaaggc ccagagttcc agccggttgg ccggcaggga
      601 aacgaagtac tgggtgccgt cttcgctcgt ggaggcgttc agcccggtgc cgccgttctt
      661 caggtaggtt tcccagagtt cgtctttgat cacgtacttt ttctcttcgg cctggagggt
      721 gtgcagctgt tcacgcaggg ctttgagttt ggtcgaatcg tgatcggaaa gcggattctg
      781 gagccgggcc tgctcggcaa tgatctggtg cgccgtctgg tcgatttttt ccatcacggg
      841 cagttcggcc ttataattgg ccgtgccgat atctttggtg cctttgaaca tcatatgttc
      901 gagcaggtgc gaggtgccgg tgatgccggg gtgctcatcc accgagccga ccttgaagcg
      961 caggtaggtc gaaaccaccg gcgcggtgtg gttctccagc accaaaacgg tcatcccgtt
     1021 tttgagcgtc tttttctgga ccggcagctc aaagccgaac gccgttccgc agaggaacag
     1081 aaacagcgcc aaaccgaaca ttgcccgcgt taacgagcgg taccgcatgt gaacctcctg
     1141 tcgaagatcc cgatttttcg gggatgatgg gtgtttccga gatgagacgt gggtgattaa
     1201 caacacgggc ggaaagtagc gcttccgcct aaatttgtca acgcggtcta attgccctaa
     1261 atacagatat gacgtttgaa agtctgacgc cgctaaatcc tgcgttttct tttgaagaat
     1321 cttttcttca cggaaaatac tcgaggtgga gcgatctatt ctcccgcccc attgccagcc
     1381 ggcttcaacc gttccttgag caggacggtt tccccctccg tcagggcgac ggagcgttct
     1441 ttgagattta agttttcccc cgcaaagtgg acgcggtggc tgccgggatc aaggttcaaa
     1501 agcaggggcg gcggcgtgct gccgcggtac ttgccatcga cgtagatgtc cgccgagggg
     1561 agaacatcaa tcaccaggta accgacgggc ttgctcgact gcggctggga tttttccttc
     1621 acgtagtacc acaaaaggag caccg
//
LOCUS       DBNGZA010000410         1443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2416105, whole genome shotgun sequence.
ACCESSION   DBNGZA010000410 DBNGZA010000000
VERSION     DBNGZA010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2416105"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16FU_08850"
     CDS             complement(<1..195)
                     /locus_tag="AC16FU_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994514.1"
                     /translation="MSTSLQPQRSHYRRLVRFLKPYWKHATVSMVSMTLFALLSGAMI
                     WMIGPLAGTLFGLGGKSVMTA"
     gene            complement(192..1319)
                     /locus_tag="AC16FU_08855"
     CDS             complement(192..1319)
                     /locus_tag="AC16FU_08855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR2994515.1"
                     /translation="MKKLEKLAKKAALRLLRLIWGNSPVPGAFRREEVRSIVVVRQDE
                     RLGNMVLITPFLAGLRKAFPGVSVTLVASEKYGHLFDGDKRIDQLVLVNKKRCLFNPV
                     YLLKTIQRLRNIRTDLAFDLSDAGQFSALNSIATRLTGARLRIGFDRPEALTFLNRLV
                     PVPEIPCHATEMHLQLLEALVPGSYDPIPKLAVNPEARKWGEGWLDRKRRVPEAKLVA
                     VHIGARGPKQWGVQSFFELIKQFTANDDVKIVLLAGPLERHLLTESSIPWPLDVRIAP
                     EVSITQLAALLSLCDLYVGADAGPLHLAAAVGVPTVSLFFTSDADKYGPQGKRHVILA
                     TPGRKPKVTEVYQSIYSVLAGEGRPAWDGKPAQVAVAEEVK"
BASE COUNT          337 a          410 c          381 g          315 t
ORIGIN      
        1 ggcggtcatg acgctttttc cccccagacc gaagagcgtt cccgccaaag ggccgatcat
       61 ccagatcatc gccccgctca aaagcgcaaa aagggtcatg ctcaccatcg acacggtggc
      121 atgcttccag tacggtttca ggaagcgaac cagtcgccgg tagtgggagc gttgcggttg
      181 cagggaagtg ctcactttac ctcttccgcg acggctactt gagccggttt gccgtcccaa
      241 gccggacgac cttctcccgc cagcaccgaa taaattgatt gatagacttc tgtgaccttc
      301 ggcttccggc ccggagtggc gagaataacg tgtctcttgc cttgcggccc gtatttgtcc
      361 gcgtctgaag taaagaatag tgaaaccgtt ggtaccccga ctgccgcggc cagatggagc
      421 ggtccggcgt cagcgcctac atacagatcg caaagggaaa gcaaagccgc taattgagta
      481 attgatactt caggagcaat ccgcacatcc agcggccaag gtattgagct ctccgtaagc
      541 agatgacgct ccagtggccc ggcgagaagg acaattttta cgtcatcgtt agcagtgaat
      601 tgcttgatga gttcaaagaa actttgcact ccccactgtt tcgggccgcg ggcgccgatg
      661 tgaacggcga ccaactttgc ctccgggacg cgtcgcttcc gatccagcca cccttccccc
      721 cacttacggg cttcaggatt aactgccagt tttggtattg gatcatacga accgggaacc
      781 aacgcctcca acaactgcag gtgcatctca gttgcgtgac aggggatttc gggcaccggc
      841 accagccgat tgaggaacgt gagggcctct ggccggtcaa acccgatccg caagcgggcg
      901 ccggtaagac gggtcgctat gctattgagc gctgagaact gcccggcgtc acttaaatca
      961 aaagcgagat cagttcggat attgcggagc cgttgaatgg tttttagaag atagaccggg
     1021 ttgaacaggc agcgtttctt attcaccaga acgagttggt caatgcgctt atctccgtca
     1081 aaaagatgac catatttttc acttgccacc agagttaccg aaacgccggg aaacgctttc
     1141 cgcaaaccgg caaggaaagg cgtaatgagc accatattac ccagacgctc gtcctgccgc
     1201 accaccacga tagagcgtac ctcttccctg cggaacgctc cgggaacggg tgaatttccc
     1261 cagataaggc gcaggagtct taaagcagcc ttcttcgcaa gtttttctaa ctttttcata
     1321 cctgttcagc gaagcggttc aattcgtcta gcctccgcgg gaacgccgat actgaatttc
     1381 ctcggtgaaa tcggcacgtc gtaattggct tcctggaatt gaagggaaaa cttttcaccc
     1441 cac
//
LOCUS       DBNGZA010000411         2470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_401206, whole genome shotgun sequence.
ACCESSION   DBNGZA010000411 DBNGZA010000000
VERSION     DBNGZA010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_401206"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16FU_08860"
     CDS             complement(<1..225)
                     /locus_tag="AC16FU_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994516.1"
                     /translation="MQKPKNLTDQMANQPGAGAPRSPRQQDRRRLPPLRREDIRFRRI
                     RTAAFWVMFILLVVWMVKILNAGRGEVTRIS"
     gene            complement(248..799)
                     /gene="hpt"
                     /locus_tag="AC16FU_08865"
     CDS             complement(248..799)
                     /gene="hpt"
                     /locus_tag="AC16FU_08865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR2994517.1"
                     /translation="MAYSLQRERPIQPIISNRALQKRVRELGRQLSRDYRTKNPLCVG
                     ILKGSFIFLADLVRHLTIPHEVDFISVASYGEATRAQGSVQLLKDLNRPVGGRHVVIV
                     EDIVDTGLTVEYIRKNLLARGPKSVAILTLLDKKGRRRVPVQLKYVGFAIPNDFVVGY
                     GLDLNEQHRNLPYIGKVAGVPLD"
     gene            complement(726..2174)
                     /gene="tilS"
                     /locus_tag="AC16FU_08870"
     CDS             complement(726..2174)
                     /gene="tilS"
                     /locus_tag="AC16FU_08870"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR2994518.1"
                     /translation="MNAVLTKAVKTVQTPHLVKPGEKVVVACSGGPDSVFLVHLFAHL
                     KPLMFLDVAVAHLNHSLRGAESDADEQFTQDLAGQLELPFYARKVDVAALAEKEHLSL
                     EACGRNERYCFFSELAEQLNAEKIATGHTADDQAETVLMHLLRGAGSRGLAGMPLLRG
                     KIIRPLLHLSKKEILAYLESQKIQYRIDRTNEKTDFLRNKIRHHLMPLLEQEYNPSIT
                     AALGRTAEILEQQENYLEWFAQEAVEDCLVSSGEEAVILDSGRFLNYDPVIQRLVVRS
                     VWQKLTGSAFGLGFETVRRVIDDANGQSRLAPLGSRFWVEHTGTQLIFFKKVTQRVEH
                     SVVLPGSYSWGNPPLSLVAEVVEREGIDLFVNGDPAVAYFDWDRLTPPFVLRSVKPGD
                     RFAPLGMGGQKKVGDFFTDLKIPRYQRAKSLVLTSNGEVVWLVGYRISDRFKVTIQTR
                     RVLKAIVRDGLFAPKGKTHPADYIQQGFTKAG"
     gene            complement(2322..2468)
                     /locus_tag="AC16FU_08875"
     CDS             complement(2322..2468)
                     /locus_tag="AC16FU_08875"
                     /inference="COORDINATES: protein motif:HMM:NF016370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HIR2994519.1"
                     /translation="MVADRKRDAALRKQGLAVLRFTDREVLTNTDGVMHAIYEQIQKR
                     LRAS"
BASE COUNT          516 a          756 c          667 g          531 t
ORIGIN      
        1 cgaaattcgg gtcacttccc cgcggccggc gtttaagatt ttcaccatcc agaccacaag
       61 taaaatgaac atgacccaga aggccgcagt gcggatgcgc cggaagcgta tatcttcgcg
      121 ccgcaaaggc ggcaggcggc ggcggtcttg ctgtcggggt gaccggggag cgcccgctcc
      181 cggctggttg gccatctggt cagtcaggtt tttcggtttt tgcaacaacg cttctctctc
      241 ctcgctttca atccaacgga acgcccgcaa ctttccctat atacggcagg ttgcggtgct
      301 gttcattgag gtccaacccg tacccgacca caaagtcgtt cggaatcgca aaccccacgt
      361 acttcagctg caccggcacg cggcggcgac cttttttgtc cagaagcgtc agaatggcga
      421 cgctcttcgg cccccgcgcc aggaggtttt tgcgaatgta ctccacggtc agtccggtgt
      481 ccacgatatc ctctacaatt actacgtgtc gtccgccgac ggggcggttc aaatctttga
      541 gcaactgcac cgagccctgc gcccgggtgg cctccccgta actcgccacc gatatgaaat
      601 cgacttcgtg cgggatcgtc aaatgccgga ctaagtcggc caggaaaatg aaactgccct
      661 tcaggatccc cacacagagc ggatttttcg tccggtagtc gcgcgacagt tgccggccta
      721 actccctaac ccgcttttgt aaagccctgt tggatataat cggctggatg ggtctttccc
      781 tttggagcga ataggccatc gcggactatt gctttgagca ccctgcgggt ctgaatggtc
      841 accttaaacc ggtcgctgat ccggtatccc acgagccaga ccacctcacc gttgctcgta
      901 agcacgagac ttttggctct ctggtatcgg ggaattttga ggtcggtgaa gaaatcgccc
      961 accttcttct ggccacccat ccccagaggg gcaaagcggt cgcctggctt gaccgagcgg
     1021 agcacaaacg gcggcgttag ccggtcccag tcgaagtaag cgaccgccgg atcgccgttg
     1081 acaaagagat cgattccctc ccgctccacc acttctgcta caagcgaaag cggcgggttt
     1141 ccccaggaat atgatccggg gagcaccacg ctgtgctcta cccgctgcgt caccttcttg
     1201 aagaagatca actgagtgcc ggtgtgctcg acccagaagc ggcttcccag aggtgccaat
     1261 cgcgattggc cgttggcatc gtcaatcacc cgccgcaccg tttcaaagcc gaggccgaat
     1321 gctgagccgg tcaacttttg ccagaccgag cggacgacca aacgttggat caccggatcg
     1381 taattgagga agcggccgct gtccagaatg actgcctcct cgccggaaga gacgagacag
     1441 tcttccaccg cttcctgcgc gaaccactcc aggtaattct cctgctgttc taagatttca
     1501 gccgttcgcc ccaaagcggc ggtgatggag gggttgtatt cttgttctaa gagcggcatg
     1561 aggtgatgtc gaattttatt gcgcagaaaa tcggtcttct cattcgtccg gtcgatccgg
     1621 tattgaattt tttgcgactc taaataggcg agaatttctt ttttagagag gtgcaggagc
     1681 gggcggatga ttttcccccg cagcaaaggc attccagcta acccgcgact ccccgccccg
     1741 cgcaacaggt gcatcaagac cgtttccgcc tggtcgtccg ccgtatggcc ggtggcgatt
     1801 ttctcagcgt tcagttgctc ggccaattcg gagaagaagc agtagcgctc attccggccg
     1861 caggcctcca gcgacaggtg ctccttttct gcgagggcag cgacatcgac cttgcgcgcg
     1921 taaaacggca attccaattg tccggctaag tcctgagtga actgctcatc ggcgtcggat
     1981 tccgccccgc gcagagagtg gttcaggtgg gcgaccgcca catccaaaaa catgagcggc
     2041 ttgaggtgcg cgaagaggtg aacgaggaaa accgaatccg gcccgccgga gcaggccacg
     2101 acgaccttct cccccggctt caccaggtgg ggcgtttgaa ccgtcttgac tgcctttgtc
     2161 agtaccgcat tcatagcgac tccgctgcta aaacataggc caaaagcccc gatttggcaa
     2221 acggggggat tcacgccata ctctgacccc tccctcgccc tcccctcgaa ggggagggaa
     2281 tactcagaac tacctcttct taaaagagat gctgagcagc gtcatgacgc tcgcaatcgc
     2341 ttttgaatct gctcataaat agcgtgcatc accccatcgg tgttggtgag cacctctctg
     2401 tccgtaaaac gaagcaccgc cagcccctgc ttccgtaaag cagcatcccg tttccgatcg
     2461 gcaacaatcc
//
LOCUS       DBNGZA010000412         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2840406, whole genome shotgun sequence.
ACCESSION   DBNGZA010000412 DBNGZA010000000
VERSION     DBNGZA010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2840406"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..>1550)
                     /gene="guaB"
                     /locus_tag="AC16FU_08880"
     CDS             complement(258..>1550)
                     /gene="guaB"
                     /locus_tag="AC16FU_08880"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR2994520.1"
                     /translation="AIALAREGGLGVIHKNMSIEAQAAEVDRVKRSEAGMIVHPITLA
                     PERPIGEALEVMRRYSISGIPVTKRGRLVGILTNRDLRFEKNLSRKIGEVMTKKNLVT
                     APEGTELEAAKAILQKHRIEKLPIVDRRKTLKGMITVKDIMKQIQHPHACKDKRGRLR
                     VGGAVGVAKDLLDRAAALVDAGVDLLVLDASHGHSKGVIEAVLKLKKRFPSVPLVAGN
                     VATGAATRELIAVGADSVKIGLGPGSICTTRVVTGTGVPQITAIMDCVEVADQHHIPL
                     VADGGIKYSGDITKAIAAGASAVMIGSLFAGTEESPGETILFEGRTFKLYRGMGSLEA
                     MKKGSRDRYAQAEEEDPEKWVPEGIEGRVPYKGSLSETVFQLVGGLKAGMGVCGAKAI
                     EELRRKAEFVQITLAGLRESHPHDVTITKEAPNYRVNP"
BASE COUNT          280 a          501 c          433 g          336 t
ORIGIN      
        1 tctgagaacc cgtcagggtc gaagacaggg acgagccggt ctacccgtat aagaaagaag
       61 tagggaggtg aaaactgagt gacctcaccc cggccctctc ctacaggaga gggcgatcgg
      121 agagaaaaat gttctctccc ctccaacctt ctcttagcag gtagcatcct tccaattcaa
      181 gaagagtatt tttctccctc ccctgtaggg gagggtaggg tggggtcaga gaaacggtcg
      241 actattccaa cggaactcta cggattgacc cggtagttcg gcgcttcttt ggtgatggtc
      301 acgtcgtgcg ggtggctttc ccgcaagcca gctaaggtga tctgcacgaa ctcggctttg
      361 cgccgcagct cttcaatcgc cttggctccg caaacgccca ttccggcctt gagcccaccg
      421 actaactgga aaaccgtctc tgagagcgac cctttgtacg gcacccggcc ctcaattccc
      481 tccggcaccc acttctccgg gtcttcctct tcggcctggg cgtagcggtc ccggctcccc
      541 tttttcatcg cttcgagcga gcccattccc cggtagagct tgaaggtgcg gccttcgaag
      601 agaatcgttt cgcccgggct ctcttcggtc ccggcaaaaa gcgagccgat cataacggcg
      661 gacgccccgg cggcaatcgc tttggtgatg tctccggagt atttgatgcc gccgtccgcg
      721 accagcggaa tgtggtgctg atcggccacc tcgacgcagt ccataatggc ggtaatctgc
      781 ggcacgccgg tgccggtgac gacccgcgtg gtgcagatcg aaccgggccc caggccgatt
      841 ttgacgctgt cggcacctac cgcgatcaac tcccgggtgg cggcgccggt ggccacatta
      901 cccgccacca gcggcaccga gggaaagcgc tttttgagtt tgagcaccgc ttcgatgacg
      961 ccttttgaat ggccgtggga ggcatctaaa accaaaagat cgactccggc atcgactaac
     1021 gccgccgccc ggtccaagag gtctttggcc acccctaccg ctccccccac gcgcagccgg
     1081 ccccgtttat ccttgcaggc gtgggggtgc tggatctgct tcatgatgtc tttgaccgtg
     1141 atcatgccct tgagcgtttt cctgcggtcg acaatcggca gtttctcaat ccgatgcttt
     1201 tgcagaatgg ctttggccgc ctccagttcg gttccctccg gggcggtgac caagttcttt
     1261 ttggtcatca cctcgccgat cttgcggctc aaatttttct caaagcgcag gtcgcggttg
     1321 gtcaggatgc cgaccagccg cccccttttg gtcaccggaa tgccggaaat gctgtagcgc
     1381 cgcatcacct ccagcgcctc gccgatgggc cgctccggag ccaaagtaat cggatgaacg
     1441 atcatcccgg cttcggagcg tttgacccgg tccacttccg ccgcttgcgc ctcgatcgac
     1501 atattcttgt gaattacccc caggccgccc tcgcgcgcca gcgcaatcgc
//
LOCUS       DBNGZA010000413         2860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1248257, whole genome shotgun sequence.
ACCESSION   DBNGZA010000413 DBNGZA010000000
VERSION     DBNGZA010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1248257"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="AC16FU_08885"
     CDS             <1..486
                     /locus_tag="AC16FU_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR2994521.1"
                     /translation="WGYLKRRLAAKGGIRRESLPLYLAEYVWRYNHRRLSFRQQTQKH
                     MGLLRLTYVAGMGLYPTGKMVISPEFDFALPFSEGLACVEVGAKSGYINKTGKFAISP
                     QFDFAWNFSEGVAPAEIGGKSGYIDKTGKFVIKSSFNEARDFSEGLACVKVGGSRYSS
                     D"
     gene            693..1703
                     /locus_tag="AC16FU_08890"
     CDS             693..1703
                     /locus_tag="AC16FU_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR2994522.1"
                     /translation="MPEAVRTTGDLEAVEKLKAAKEIIKKEIGKVIVGQERVIDELMI
                     ALLSGGHCLIIGVPGLAKTLLISTLAKVLDLKFSRIQFTPDLMPSDITGTEILEEDRT
                     TGKRLFRFVKGPVFANIVLADEINRTPPKTQAALLQAMQEYEVTASGETFKLGLPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMFNINIDYPSSAEEAEIVRTTTSAYAASLDKVATG
                     EEIIRLQKLARRVPVADHVINYAVRLVRATRPVENGTYEYIKNWISWGAGPRASQYLI
                     LGGKVKAILDGRLTPSTEDVRFVAPPVLRHRIVTSFNAEAEGVGTEQIVQRLLSDVQD
                     KP"
     gene            1719..2126
                     /locus_tag="AC16FU_08895"
     CDS             1719..2126
                     /locus_tag="AC16FU_08895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2994523.1"
                     /translation="MSGIISLRFSESKDIDVDKLIDLYQFGWWSNQRKKPDVKKMLDH
                     TPMVISVWDGDLLVGFTRLLTDCTYRATIWDVIVRPEYQSQGIGRKIMEYILTHPRLK
                     TVTFFWLSTRDKHAFYEQFGFVRDQTDSLILKR"
     gene            2143..>2860
                     /locus_tag="AC16FU_08900"
     CDS             2143..>2860
                     /locus_tag="AC16FU_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR2994524.1"
                     /translation="MPTPSAASQDYRRFLVPEVISKLKGMELKARLVVEGFIAGLHRS
                     PYHGFSVEFAEHRQYMPGDPVRNIDWKVFAKSDRYYIKEFEEETNLKAYLLLDTSGSM
                     GYSSQDQSPEVKSRITKLEYASYLAAALSYLMLLQRDSVGLVTYADKIKRYIPPKAKR
                     DHLHVLLRELDGALPEKTTNTATALHEMAERIKRRGLIILLSDLFDDPDRVLSGLKHF
                     RHRKHEVILFHILDPLERTFA"
BASE COUNT          679 a          799 c          751 g          631 t
ORIGIN      
        1 tggggttacc tcaagcgccg cttggccgcc aaaggtggca tccgtcgaga aagcctgccc
       61 ctctaccttg cggaatatgt atggcgctac aatcaccggc gtttatcttt ccgacaacag
      121 acccaaaagc acatgggact gctgagactc acatatgtgg ctggaatggg actttaccca
      181 acaggaaaaa tggtgatcag tccagaattc gatttcgctt tgcctttttc tgaaggcttg
      241 gcatgtgtag aggttggagc aaaatcaggt tatatcaata aaaccggaaa gtttgcaatc
      301 agtcctcagt tcgattttgc ctggaatttt tccgaggggg tggctcccgc tgaaattggt
      361 ggaaaaagtg gttacatcga caagacagga aagtttgtaa taaaaagtag ttttaacgaa
      421 gcacgggatt tttcagaggg attggcgtgt gtgaaggttg ggggcagtcg gtatagtagc
      481 gattaaattg atttcatcat gccaacgcct ttcgtatcga gtgtctttaa tcaataacac
      541 cctaaaagta aaaagtccac tgaatcacta aacatctatc ctttccatca aaccattcgg
      601 atgagctcga aattgtgcca ttgccagaac gccttttgat acgtaccatt actgggttcg
      661 cgagctttta gaatgagcaa tttggaggac taatgcctga agcagtcaga accaccggcg
      721 atttggaggc ggtcgaaaaa ctcaaagccg ccaaagagat aatcaaaaag gaaatcggca
      781 aagtcatcgt cgggcaggag cgggtgatcg acgaacttat gatcgccctc ctctccggcg
      841 ggcactgcct cattatcggc gttcccggct tagccaagac gcttttaatt tcaactctgg
      901 cgaaggtgct cgacctcaag ttttctcgca tccagttcac ccccgacctg atgccctccg
      961 acatcaccgg caccgagata ttggaagagg acaggaccac cggcaagcgg ctcttccgct
     1021 tcgtcaaggg gccggttttt gccaatatcg tcttagccga cgagatcaac cgcaccccgc
     1081 ccaaaaccca ggcggctctc ctgcaggcga tgcaggagta cgaggtgact gcctccggcg
     1141 agaccttcaa actcggtttg ccctttttcg ttttggcaac gcagaacccg attgagcagg
     1201 agggcactta tcccctgccc gaggcgcaac tcgaccgctt tatgttcaac atcaatatcg
     1261 actacccatc ctcggccgag gaagccgaaa tcgtgcggac aaccacctcc gcctatgccg
     1321 ccagtctgga taaagtggcg acgggcgagg aaatcatccg tctgcagaaa ttggcccgcc
     1381 gggtgccggt ggccgaccat gtgattaact acgcggtgag gttagtccgg gcgacgcggc
     1441 cggtggagaa cggcacctac gagtacatca aaaactggat ctcttggggc gccggcccgc
     1501 gcgcgtcgca gtatttgatc ttgggcggca aagtgaaggc gattttggat ggccggctga
     1561 cgccttcgac cgaagacgtc cgcttcgtcg ctccgccggt cctgcgacac aggattgtga
     1621 ccagtttcaa tgccgaagcc gagggcgtgg gaacggagca aatcgtccag cggctcctct
     1681 ccgacgtaca ggataaaccg tgatttagcc taacggctgt gtcaggaata atttctcttc
     1741 gcttttcaga atccaaagac atcgatgtcg acaagctcat cgacctttac cagttcggct
     1801 ggtggagcaa ccagcggaaa aaacctgatg tcaaaaaaat gctcgaccac acgccaatgg
     1861 taatttccgt ctgggacggc gacctcttgg tcggcttcac ccggcttttg acggactgca
     1921 cctatcgggc aaccatttgg gatgtcatcg tccggccgga ataccagagc cagggcatcg
     1981 gtagaaaaat tatggaatac atcctgaccc atccgaggtt aaagaccgtg accttcttct
     2041 ggctttccac gagggataag catgctttct atgaacagtt cggcttcgtc cgcgaccaga
     2101 ccgacagcct gattttgaaa cgctgagcca gattctgccc ttatgccgac cccgtccgct
     2161 gcctcgcagg attaccgccg cttcttagtg ccggaggtga tctccaaact caaggggatg
     2221 gaactgaagg cgcggctggt ggtggagggg ttcatcgccg gcttgcaccg ctccccctac
     2281 cacggctttt cggtggagtt cgccgaacac cgccagtata tgccgggaga cccggtgcgc
     2341 aacatcgact ggaaagtctt cgccaaatcg gaccgctact acatcaaaga gttcgaggaa
     2401 gaaaccaacc tcaaggccta cctgcttctg gacacttcgg ggtcgatggg gtactcaagt
     2461 caggatcaat ctcccgaggt caaaagtcgg ataaccaaac tggaatatgc ctcctacctg
     2521 gccgcggcgc tttcctatct gatgctcctc cagcgcgatt cggtggggct ggtcacctat
     2581 gccgataaga tcaagcgtta catcccaccc aaggccaaac gcgaccacct gcacgtcctt
     2641 ttgcgggagt tggacggtgc cctgccggag aagaccacca acaccgctac tgctctgcac
     2701 gaaatggcgg agcggatcaa aaggcgcggg ctcattattc tcctttccga cctctttgac
     2761 gatcccgacc gggtgctctc gggactcaag cacttccgcc accgcaagca cgaggtgatt
     2821 ctcttccata tcctcgaccc gctggaacgc accttcgcct
//
LOCUS       DBNGZA010000414         1935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1777795, whole genome shotgun sequence.
ACCESSION   DBNGZA010000414 DBNGZA010000000
VERSION     DBNGZA010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1777795"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="AC16FU_08905"
     CDS             <1..809
                     /locus_tag="AC16FU_08905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR2994525.1"
                     /translation="VASGPCQWKVTEKNLAKILGAPIYIPDMAEAKPEVGVATGLAWT
                     ETGGDIMLIEALKMKGSGTVISTGSLGDVMKESIQASHSYVRAKADLLGIRYEDFTSH
                     DIHIHFPSGAVPKDGPSAGIAISLAIASVMANKAIRNDIAMTGEVSLRGKVLPVGGIR
                     EKVSAALRVGIRTVVLPKQNEKDLEDLPPRLRKEMKFIPIEYTEEIFPLAFVDFQPGV
                     ASLEQLLRQEVEKVRKKKTKENARERKAAKSARSRKPQPPEEPTGSISFV"
     gene            979..1833
                     /locus_tag="AC16FU_08910"
     CDS             979..1833
                     /locus_tag="AC16FU_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR2994526.1"
                     /translation="MVIGERLASYARFVKIEHTLFSLPLIYAGALLARPADKPWPVFL
                     LILTAAVGARSAAFGLNRIIDLAIDRANPRTQNRELPAGRMRLREAWLVTLAGSVIYF
                     ISAGLLGKPCLVLAPLPLIIFVTYPYLKRLTPLAHFGVGLGLAMAPLGGWFAIRQEFV
                     GLGPPILLGLFTWLWVSGFDIIYSTADEDFDRAHRVHSLPGRLGKKRALAVSGLLHFL
                     AFLCLVALYSRFFRSFLALPFLLLTGVLLFLEQRKAHDVELAFFKINAVLGFVVLLLV
                     GVGVSVQP"
BASE COUNT          344 a          585 c          575 g          431 t
ORIGIN      
        1 gggtggcctc cggcccctgc cagtggaagg tgaccgagaa gaacctcgcc aaaattctgg
       61 gggcgccgat ctacattccc gatatggcgg aggccaagcc ggaagtgggg gtggccaccg
      121 gtctggcctg gaccgagacc ggcggcgaca tcatgctgat cgaagcgctg aagatgaaag
      181 gctccggcac ggtcatctcc accggctctc tgggtgacgt gatgaaagaa tcgatccagg
      241 cctcccactc ctacgtgcgc gccaaagccg acctattggg catccgctac gaagatttca
      301 cctcccacga catccacatc cacttccctt cgggagcggt tcccaaagac ggcccctcgg
      361 ccgggatcgc catatcgctc gccatcgcgt cggtgatggc caacaaggcg atccgcaacg
      421 acatcgccat gaccggcgag gtgtctctgc ggggcaaagt gctgccggtg ggggggatcc
      481 gggagaaagt ctccgccgcg ctccgggtgg ggatccggac ggtggtcctg ccgaagcaga
      541 acgaaaaaga tttggaagat ctgccgccgc gcctgcgcaa ggagatgaaa ttcattccca
      601 tcgagtacac cgaagaaatc ttccctttgg cgttcgtgga cttccagccg ggtgtggcca
      661 gtctggagca actgctgcgc caggaagtgg aaaaagtccg gaagaaaaaa accaaggaaa
      721 acgcccggga gcgcaaagcc gccaaatcgg cccgcagccg gaaaccgcag ccgccggaag
      781 agccgaccgg ctcaatcagc tttgtttgaa tttttctccc ggagtcccct cctttccttc
      841 ccaaagtcag ccctcctttt cgttactgga gttaccaccg tctgccgaag agcggcacgg
      901 gaaagagaat tgacttcccg gtcactgcgt tgtagcgtat gcaggcaaag cgattgatag
      961 ggaagagagt tgattccgat ggtgataggg gaacggctgg ccagctacgc gcggtttgtc
     1021 aaaatcgagc acaccctttt ttccctgccg ctcatttacg ctggtgcgct tctggcccgg
     1081 ccggcggata aaccgtggcc ggtttttctc ctcatcctga cggcggccgt cggcgcccgc
     1141 agcgccgctt ttggtttgaa ccgtataatt gatttggcca tcgaccgcgc caatccgcgc
     1201 acgcagaacc gcgagttgcc ggcgggccgg atgcggctcc gtgaggcctg gctcgtaaca
     1261 ctggccggta gtgtcatcta tttcatctcc gccgggcttc tgggcaaacc gtgtctggtc
     1321 ttggcgcccc tcccgcttat tattttcgta acctatccct acctgaagcg cttaactccc
     1381 ttggcgcatt tcggcgtcgg tttggggctg gccatggcgc ctctgggcgg gtggtttgcc
     1441 atccggcagg agttcgtcgg gttgggcccg ccgattcttt tgggtctttt cacctggctc
     1501 tgggtgtccg gttttgacat tatctactcc accgccgacg aagattttga ccgagcccac
     1561 cgggtccatt ccctccccgg ccgtctgggt aaaaaacggg cgctggccgt ctccggcctt
     1621 ttgcactttc tggcgttcct ctgtctggtg gcgctgtatt cccgcttttt ccgcagcttt
     1681 ctggcgctgc cgttcctttt gcttacgggc gtccttctgt ttttagaaca acgtaaagcg
     1741 cacgatgtgg aactggcctt cttcaaaatc aacgcggtgt tgggcttcgt ggtcctgctt
     1801 ttggtcggcg tcggcgtgag cgtacagccg tgaggatcgt cgtcggcatt accggcgctt
     1861 ccggggcgat cttcagcgtc gattttctca aacgctgccc cggtgagaag tatctcgtct
     1921 tctcccagtg ggggc
//
LOCUS       DBNGZA010000415         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_401595, whole genome shotgun sequence.
ACCESSION   DBNGZA010000415 DBNGZA010000000
VERSION     DBNGZA010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_401595"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1096
                     /locus_tag="AC16FU_08915"
     CDS             122..1096
                     /locus_tag="AC16FU_08915"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
                     /protein_id="HIR2994527.1"
                     /translation="MRVIIPVAGGGTRLWPLTQRTPKVLLQVADRPMLGHILEQVEKV
                     EPEEVIFIIGAGGDAIINYVVRGYDFPVRFVEQTELDGLGYAVALAIPFFGCGPLLII
                     LGDTLLGADLKKIVRTDHDALGVKEVNDPQRFGIAEVTRGVITRLVEKPQAPQSNLAL
                     VGLYFIRSVELFSNCLAELLAGGVRTRGEYQLTDALQKMIERGCQFRSFPIHEWLDCG
                     KPETLLATNRHLLRNGSDNAGPKTKGIIPPVFVAPDAVVTGSRLGPFVSIGSRARIKD
                     AVLADVICGSEASVSGVTLEHAIVGNGATVHGQIPVRKTKDEILLIQN"
     gene            1112..2275
                     /gene="metK"
                     /locus_tag="AC16FU_08920"
     CDS             1112..2275
                     /gene="metK"
                     /locus_tag="AC16FU_08920"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR2994528.1"
                     /translation="MPRKNFLFTSESVTEGHPDKVCDQISDAVLDEVIRQDRTGRVAC
                     ETFITIGLVIVGGEITTKGYVDVPNLVRNVIRDIGYTDPKYGFNCDTCAVLNAIGAQS
                     PDIAQGVDTGGAGDQGQMVGYACRETPELMPLPIALAHRLTRRLAEVRKKKILPYLGP
                     DGKAQVTVQYDNGTPAKVTSVVLAAQHTEEILDATGKKITEEARQEIIEAVVKPVIQP
                     ELMDKQAKLYVNATGKFVIGGPQCDTGMTGRKIIVDTYGGMAAHGGGAFSGKDPSKVD
                     RSASYMARYIAKNIVAGGLAERCTVQLAYAIGVAEPVSVMVDTEGTGKLDDLRLTEMV
                     KMHFALTPRGIIEALNLLRPIYQKTASYGHFGRDDADFTWEKTDKIKDLQKEA"
     gene            2324..3577
                     /locus_tag="AC16FU_08925"
     CDS             2324..3577
                     /locus_tag="AC16FU_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR2994529.1"
                     /translation="MRYDVKDKKLARTGKLRVEWAERNMPVLRLIRKRFAREKPLKGL
                     RIAACLHVTTETATLMETLVAGGAQVALCASNPLSTQDDVAAYLVTRRIPVFAVKGEN
                     NKTYYRHIQDTLNIKPQITMDDGADLVSTLHTERPEEAEKILGGTEETMTGVTRLRSM
                     ERSGVLRYPIVAVNDSKTKYLFDNRYGTGQSTIDGILRATNMLLAGARVVILGYGWCG
                     RGVAMRAKGMGADVIICEVNPHRALEAVMDGYRVMSSGEAAREGNLFVTVTGDINVLR
                     SEHFRAMNDGAVVANAGHFNVEIDLASLVKLARRRRAVRQYIEEFTLNGGKKVNVLAD
                     GRLINLSAAEGHPAMVMDMSFANQALSVEFIAKSGHELERKVYVVPEEIDRQVAELKL
                     KSMGVKIDRLTEEQKNYLSSWEMGT"
BASE COUNT          796 a         1035 c         1155 g          674 t
ORIGIN      
        1 ggagcgtgct ttagcacatc tgctccgtgg ttcgccttgc gtaattctct tccaaagagt
       61 aaattgcccg cgcttcccga acggttatcc aactggaagg tgaagaggcg gttgatctat
      121 tatgagggtg attattccgg tggctggagg cggaacgcgg ctctggccgt tgacgcagag
      181 aacgcccaag gtgctcctgc aggtagccga ccggccgatg ctgggacata ttctggagca
      241 ggttgaaaag gtggaacccg aagaagtgat tttcatcatc ggcgccggcg gggacgccat
      301 catcaactac gtggtgcgcg gctacgattt tccggtgcgt ttcgtggagc agacggaact
      361 ggacggtctg ggttacgcgg tggcgttggc gatcccgttt ttcggctgcg gaccgctttt
      421 gatcattctc ggcgacaccc tcctcggcgc ggatttgaaa aaaatcgtgc ggaccgacca
      481 cgatgccctg ggcgtcaaag aagtgaacga tccgcagcgc tttggcattg cggaagtgac
      541 gcgcggcgtc attacgcggc tggtggagaa accgcaagcg ccacagtcca acctggcttt
      601 agtcggattg tacttcattc ggtcggtcga actcttctcg aactgcttag cggagctttt
      661 ggccgggggc gtccgcaccc ggggagagta ccagctcacc gacgcgctgc agaagatgat
      721 cgagcgcggc tgtcagttca gatcgtttcc aattcacgag tggctcgatt gcggcaagcc
      781 agaaacgctt ttggccacca accgccatct gctgcggaac ggttcggata acgcgggacc
      841 gaagacgaaa ggtatcatac cgcccgtttt tgttgcaccc gatgcggtgg tgacgggctc
      901 ccgcctgggg ccgttcgtct cgatcggcag ccgcgcccgg atcaaagacg cggtgctcgc
      961 ggacgtcatc tgcggcagtg aggcttccgt cagcggcgtc actctggaac atgccatcgt
     1021 tggcaacgga gcgaccgttc acggccagat ccccgtccgc aaaacgaaag atgaaatcct
     1081 gctcatccag aactaacctt caggagaggg aatgcccagg aaaaatttct tattcacttc
     1141 agaatcggta accgaaggcc atcccgacaa agtctgcgac cagatttcgg acgcggtctt
     1201 ggacgaagtg attcgccagg accggacggg ccgggtggcc tgtgaaacct tcatcaccat
     1261 cgggctcgtg atcgtcgggg gtgagatcac caccaaaggg tacgtggacg tgccgaattt
     1321 agtccgcaac gtgatccgtg acatcggcta caccgacccg aagtacggct tcaactgcga
     1381 cacctgcgcg gtgttgaacg ccatcggggc gcagtcgccg gacatcgccc agggtgtcga
     1441 taccggcggg gcgggagatc aggggcagat ggtgggctac gcctgccggg aaaccccgga
     1501 gctgatgcct ttacccattg ctttagccca ccggctgacc cggcgtctgg cggaagtgcg
     1561 caagaaaaaa attctcccgt acctgggccc ggacggcaag gcgcaagtaa cggtacaata
     1621 cgacaacggc acgcccgcca aggtgacctc cgttgtcctg gccgcgcagc ataccgaaga
     1681 aattctggac gcgaccggca agaagatcac cgaagaagcg cggcaggaaa tcattgaggc
     1741 cgtggtcaag ccggtcatcc aaccggagct gatggataag caggcgaaac tctacgtgaa
     1801 tgccaccggt aagttcgtca tcggcggccc gcagtgcgac accggcatga cggggcgcaa
     1861 gatcatcgtc gatacctacg gcgggatggc ggcccatggc gggggcgctt tctccggcaa
     1921 agatccctcg aaggtcgacc gttccgcatc ctacatggcc cgctacatcg ccaaaaacat
     1981 cgtggctggg gggttggccg agcgctgcac cgtgcagtta gcctacgcca tcggtgtggc
     2041 cgagccggtc tctgtcatgg ttgataccga agggaccggc aagttggacg acctgcgcct
     2101 gaccgaaatg gtcaaaatgc attttgccct caccccgcga ggaatcattg aggcgctcaa
     2161 cctcctgcgg ccgatctatc agaaaaccgc gagctatggt cacttcgggc gggacgatgc
     2221 cgatttcacc tgggaaaaga cggataagat caaggattta cagaaagagg cttgagagcg
     2281 cgagcgtgaa gtcggagtgc cgcgaaaacg aaagcgagga gtaatgcgtt acgatgtgaa
     2341 ggataaaaaa ctggcccgta cggggaaatt gcgggtggag tgggcggaac ggaacatgcc
     2401 ggtcctccgc ctcatccgca agcgttttgc gcgggagaaa ccgctcaaag ggctgcgcat
     2461 cgccgcctgc ctgcacgtca ccaccgaaac ggccacgtta atggaaacgc tcgtggccgg
     2521 cggagcgcaa gtggcgctct gcgcttccaa ccccctctcg acgcaggacg acgtggcggc
     2581 ctacctggtg acccggcgga tcccggtctt tgccgttaag ggggaaaaca acaagaccta
     2641 ctaccggcac attcaagaca cgctcaatat taaaccgcag attaccatgg atgacggcgc
     2701 cgatctggtc tcgaccctgc acacggagcg acccgaagaa gccgaaaaga tcctcggcgg
     2761 caccgaggag acgatgacgg gcgtcacccg cctgcggagc atggaaaggt cgggggttct
     2821 gcgctacccg atcgtcgcgg tcaacgactc gaagacgaag tacctgttcg acaaccgcta
     2881 cggcacgggt caatcaacca tcgacggcat tctgcgggca actaatatgc ttttggcggg
     2941 tgccagggtg gtcatcctgg gctacggctg gtgcggccgg ggtgtggcga tgcgggcgaa
     3001 aggaatgggg gccgacgtca tcatctgtga ggtcaatccc caccgggcgc tggaagcggt
     3061 gatggacggg taccgggtca tgtcttcggg cgaggccgcc cgcgaaggca atctatttgt
     3121 caccgtcacc ggcgacatca atgtgctgcg ttcggagcat ttccgcgcga tgaatgacgg
     3181 agcggtggtg gccaacgccg ggcacttcaa tgtcgaaatc gatttggcgt cactggtgaa
     3241 attggcccgc cggcgccggg ccgtgcgcca gtatatcgaa gagttcacgc tcaatggcgg
     3301 caaaaaggtg aacgttctgg ctgacgggcg gctgatcaat ctttcggcgg cggaaggcca
     3361 cccggcgatg gtgatggata tgagttttgc caaccaggcc ttgagcgtcg agttcattgc
     3421 caaaagcggc cacgagctgg agcgcaaggt ctacgtcgta cccgaggaga tcgaccggca
     3481 ggtggcggag ttaaaactga agtcaatggg cgtcaaaatt gatcggctca ccgaagaaca
     3541 gaaaaattac ctctcctcgt gggaaatggg aacgtagggt tttcgaaacc ctcacccacc
     3601 gggccgcagg gtaccccgcc gtcgtgcttc ctccggctga aacgtacgca ctgctccgcc
//
LOCUS       DBNGZA010000416         1439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_186669, whole genome shotgun sequence.
ACCESSION   DBNGZA010000416 DBNGZA010000000
VERSION     DBNGZA010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_186669"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1439)
                     /locus_tag="AC16FU_08930"
     CDS             complement(<1..>1439)
                     /locus_tag="AC16FU_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2994530.1"
                     /translation="RSRTFESLHLLQSGIDGNARGQVVFGSKNNEKDNLYVYDIPRHT
                     ITLAAGFDSLVGVLSPVWIPGRQAVVFTGVDKGGFSDLFLYDLESRSLRRLTHDYYED
                     TDPAVAPDGQEVAFSSDRGSSGKSGTKNIFVLDLATGVITPRTSGAHDDLTPSWSPDG
                     RWIVFTSDRNGPANVFAVNDSNQSVQLTNVLTGVSDPKFLPDGRHLLFTAFKDFSFQI
                     FSTPLDSTLWQKPAPEPQIPVIAWVPDTLSGNLAQGAVKYQKDFSFDIAQSAVAYDNV
                     FGTVGGFQTAFTDILGNQQLYFLLSNNARTRKQFFSSFNAGVTYVNKTHRVNFGAGAF
                     HFYDEYYDDFDGFIAERQVGGLVFASYPVSKFQRFETSFFLRYSNKDFQYFFDRRKAL
                     LATNYLSWIRDNSLWDVVGPIDGHRYNLTVGLTSDLTTGRQYNRSVLADGRRYFRLGR
                     HSALALRLFGFSSTGLEPQRLYLGGSWSL"
BASE COUNT          312 a          389 c          481 g          257 t
ORIGIN      
        1 gcaacgacca ggagccgccc aggtagagcc tttggggctc caggccggtg gaggaaaaac
       61 caaagagccg cagcgccagg gccgaatgcc ggcccaagcg gaagtagcgc cggccgtcgg
      121 ccaggacgct ccggttgtac tgccggccgg tggtgagatc ggaggtcaga cccaccgtaa
      181 ggttgtagcg gtgcccgtct atcggcccca ccacgtccca gagactgtta tcccggatcc
      241 aggagaggta gtttgtggcc aaaagcgcct tccgccggtc gaagaaatac tggaagtcct
      301 tgttggagta acggagaaag aaactcgttt cgaaccgctg gaacttggaa accggatagg
      361 aagcgaagac gagcccgccc acctggcgct cggcgatgaa gccgtcgaaa tcgtcgtagt
      421 actcatcata gaaatggaag gcgccggccc cgaagttcac ccggtgggtt ttattcacgt
      481 aggtgacgcc ggcgttgaaa ctggagaaga actgcttgcg ggtgcgggcg ttgttggaga
      541 gcaaaaagta gagctgctgg ttgcccagga tgtcggtgaa ggcggtctgg aacccgccga
      601 ccgtaccgaa gacgttgtcg taggccaccg ccgactgggc gatatcaaag gaaaaatctt
      661 tctggtattt gaccgcgccc tgcgccaggt tgcccgagag ggtgtcgggc acccaggcga
      721 tcacgggtat ctgcggttcc ggagccggct tttgccagag ggtgctgtcc agcggggtcg
      781 agaagatctg gaaggaaaaa tctttgaagg cggtgaaaag caggtgccgg ccgtccggca
      841 ggaacttcgg gtcggagacg ccggtgagga cgttggtcag ctggaccgat tgattcgaat
      901 cgttcacggc gaaaacgttg gccggcccgt tccggtcgga ggtgaaaacg atccagcgcc
      961 cgtcgggcga ccaggagggt gtcaggtcgt cgtgggcgcc ggaggtcctg ggggtaatga
     1021 cgccggtagc cagatccaga acgaaaatat tttttgtgcc cgatttcccc gacgaacccc
     1081 ggtcggaact gaaggccacc tcctgtccgt cgggcgcaac ggcgggatcg gtatcctcgt
     1141 agtaatcgtg ggtcaggcgc ctgagactcc gactttccag gtcgtacaag aaaaggtccg
     1201 aaaaaccgcc cttgtcgacg ccggtgaaaa caactgcctg ccggcccggg atccagaccg
     1261 gtgagagaac gcccacgagc gagtcaaaac cggcggccag agtgatcgtg tgcctgggga
     1321 tgtcgtaaac gtagaggttg tctttctcgt tgtttttgga accgaacacc acttgcccgc
     1381 gggcattacc gtcaatgccg ctctgcagca gatggagcga ttcaaacgtc cgggagcgc
//
LOCUS       DBNGZA010000417         4476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2627650, whole genome shotgun sequence.
ACCESSION   DBNGZA010000417 DBNGZA010000000
VERSION     DBNGZA010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2627650"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="AC16FU_08935"
     CDS             complement(<1..1089)
                     /locus_tag="AC16FU_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR2994531.1"
                     /translation="MRNQTVIPKVAPEKAKPQASRTARVVVLKTKPETVLDDYTRLLE
                     QAQSERLLDPRKDLILKLNLSWTKYFPACSTQPWQLEAVLRYLLAKGFDRNKLFPLEN
                     KTVVTKPVEGARLNRWLPVLKKFGLPFIPLPDQGWVKYDFRNELVHLKKLFPEGIEIP
                     ALYPGTQVLHLPTMKTHGHSVTTGAVKNAFGGLLKEVRHYAHKYIHEVLVELMVMQKE
                     LHPKVFAVMDGTVAGNGAGPRTMQPVEANFLLASADSVAIDAIAAKIMGFDPLSIPYL
                     RICHERGLGIADPKEIEVVGEDIRGVNLHFTVKKSLVIWGDQMLRIGPLRFLEKVALH
                     SPLVVWAPFASNFYHDWIWYPTVGKAIIR"
     gene            complement(1249..2169)
                     /locus_tag="AC16FU_08940"
     CDS             complement(1249..2169)
                     /locus_tag="AC16FU_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994532.1"
                     /translation="MKLFSFLSERTQKVPAAGEAEPGGNAPTPGEGLPAHWHALAGRR
                     SLAEEKWKEAAQVAAAGIALNPDAYDLYELLLLAYRGSGRTEPKPKEVKKFLEQVSSG
                     PWRSILSGIKLVFEENFQEAWAELSPLPEHFPGSRLFPAIRALCAATLLTNHRPQLEH
                     PAQVRHRLQQDLSACRAADEHWSGAVKLFEEKNYRALLTAITRLFAERCTFPPLDAVE
                     KIHLEFLARPENFDFSEPVSSPTLAEDAPPGKFLAAELFSFLADCQKAEGEFASACQL
                     SPQFAKAQNNLTLIREQKAVLVSVLKDLRL"
     gene            complement(2166..3305)
                     /locus_tag="AC16FU_08945"
     CDS             complement(2166..3305)
                     /locus_tag="AC16FU_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994533.1"
                     /translation="MENSVTVTTAAGPEAPAVLWEEFFRTGPVDVLAQTEYDETEGVF
                     ICQLFRNRRLVGARRSVPVDPAGSFHDSGGAFHQREVLFLKTLFYLAKRLEKQGTATE
                     ETSVGQAFLVLGFFPEAEACFRWALERDPDSSEAHFRLAGVCHRLGKCAEAEALYRKA
                     LSLQPYADYANALGLLQLEGGKVKEAEEAFRQALEINPNYAEALLNLGHLYLEQAGSG
                     KGRATDECFAAAEVYLSRAHLIAHAATSGADTKKEHFDRYEALCRIYLKTKNQLVAQH
                     DSSNRRALCEVYLLALKYLAHELSAEQIGQFYFHLLDATNGKPDYPDLKNYAGLIVIF
                     SGLVALEKAALEIVRQEKDERALELVLPIQQVLTATRTLKEKLAT"
     gene            complement(3440..4150)
                     /locus_tag="AC16FU_08950"
     CDS             complement(3440..4150)
                     /locus_tag="AC16FU_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR2994534.1"
                     /translation="MDDIRISLETAGPQNEVAIVRVDGVVDTLTASELEKVMNSLFEQ
                     KRFNIVVDLGGVDYISSAGWGIFISNIREIRRNAGDIKLARMVPSVYEIFELLEFDSI
                     LKAFDNVEKAKGDFGLDQKPKAAAPPAPSLPVSRPGAEAFRLPAGPAPVGSPARAGTS
                     VTIPVTRTPEPQAWVDAVLSLVGEDPFYSIAEIARELRSRFGYPGASWWQVFRLLASR
                     KLLRKKSRFRYQRMRSRN"
     gene            complement(4126..>4476)
                     /locus_tag="AC16FU_08955"
     CDS             complement(4126..>4476)
                     /locus_tag="AC16FU_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR2994535.1"
                     /translation="DVRQAGHPIEPLGILATFYDGRTNIAKRLWNELLECYGEKVLAT
                     RIRVNTALKEAQAVQKTIFEYSPYAHGAIDYFLLTREILAHPRLQPAPQLPGEHTEDT
                     AEPRASVWMTSASL"
BASE COUNT          894 a         1353 c         1286 g          943 t
ORIGIN      
        1 gcggattatg gctttcccga cagtggggta ccaaatccaa tcgtggtaaa agttggaggc
       61 aaagggcgcc cagactacta agggcgaatg cagcgccact ttttctaaga agcggagcgg
      121 gccgatccgc agcatctggt cgccccagat caccagactt ttctttaccg taaagtgcag
      181 gttgaccccg cggatatctt cgccaacgac ttcgatttcc ttcggatcag caatccccag
      241 tccccgctcg tggcagatcc gcaggtaggg gattgacaga gggtcgaatc ccatgatctt
      301 cgccgcaatg gcgtcgatgg ccaccgaatc ggccgacgcc agaaggaaat tggcctcaac
      361 cggctgcatg gtgcgcggcc cggcaccgtt gcccgccacc gtgccgtcca tgaccgcaaa
      421 gactttcgga tgcagttcct tctgcatgac catcaactcc accagcacct cgtgaatgta
      481 cttgtgggcg tagtgacgaa cttctttcaa taggcccccg aaagcattct tgaccgcgcc
      541 ggtcgtgacc gagtgcccgt gcgttttcat ggtgggcagg tgcagcacct gcgtgcccgg
      601 gtagagggcc ggaatctcaa tcccttcggg aaaaagtttt ttgagatgga cgagctcgtt
      661 cctaaaatcg tactttaccc acccctggtc gggcaaggga ataaagggta gaccgaactt
      721 tttgagcacc ggcagccagc gattgagccg cgctccttcc accggcttgg tgacgacggt
      781 cttgttttcc agcgggaaca gtttgttccg gtcgaaaccc ttcgccagca aatagcgcaa
      841 caccgcttcc aactgccacg gctgcgtcga gcaggccgga aagtatttgg tccaggagag
      901 gtttaatttg aggatgaggt ctttccgggg atcgagcagg cgctccgatt gcgcctgttc
      961 cagcagtcgg gtgtaatcgt ccagcaccgt ttccggcttc gtcttgagca cgaccacccg
     1021 agcggtccgc gacgcctgcg gctttgcctt ttccggcgca accttcggta tgaccgtctg
     1081 atttctcata gcaactgatc gtccggtaaa atctctcttg ttcaaaaagc taaaataccc
     1141 aaaaaccata ctattgggta gcgccggtct ctttagcctg agcgaaccgg aggcctgacc
     1201 agcctgcccg ccatttcgtt tttaggggac aacctcgggc gatttggctt atagccggag
     1261 atccttgagc accgacacca gcactgcttt ttgttcccga atcaaagtga ggttgttctg
     1321 ggctttggca aactgcggcg agagctgaca ggcactggca aactcccctt cagctttttg
     1381 acagtctgcc aggaaagaaa agagctccgc cgccaggaat ttccccggcg gagcatcttc
     1441 cgccagcgtc ggcgaactaa ccggctcgct gaaatcaaaa ttttcaggcc gcgccaaaaa
     1501 ctccagatgt attttttcga cggcatcgag cggagggaac gtacagcgct cggcaaacag
     1561 gcgggtaatg gccgtgagca gggcgcgata atttttctcc tcaaacagct tcaccgcccc
     1621 gctccaatgc tcatcggcgg cccgacaagc ggatagatcc tgttgcagac ggtgcctcac
     1681 ctgcgccggg tgttccagct gcggccggtg gttggtcagg agcgtcgctg cgcaaagggc
     1741 tcgaatcgcc ggaaaaagcc gactgccggg aaagtgctcc ggcaagggtg aaagctccgc
     1801 ccaggcttcc tggaaatttt cctcaaacac cagcttaatt ccggaaagga tcgaccgcca
     1861 gggacccgaa ctgacctgct ctagaaattt ttttacctct ttgggcttcg gttcggtccg
     1921 gccgctcccc cggtaggcca aaaggagtaa ttcgtagagg tcatacgcgt cgggattaag
     1981 ggcaatgcca gcggcggcaa cctgagccgc ctctttccat ttctcttcgg ctaacgaccg
     2041 ccggccggct aaagcatgcc agtgcgccgg cagaccttct ccgggggtag gagcgttccc
     2101 accgggctcg gcctccccgg ccgcgggaac cttctgcgtt cgctcactca ggaaggaaaa
     2161 taatttcatg tcgccagctt ttccttgagc gttctcgtcg ccgttaaaac ctgctgaatg
     2221 ggcagtacca gttccagcgc ccgttcatct ttctcctgcc gcacgatctc taaggccgct
     2281 ttttccagcg ccaccaaacc ggaaaagatg acaatgagcc cggcgtagtt cttcaggtcg
     2341 gggtaatcgg gtttcccgtt ggtggcgtcg agcagatgaa aatagaactg gccaatctgc
     2401 tcggcggaga gctcgtgcgc aaggtatttg agggctaaga ggtagacttc gcacagtgcc
     2461 cgccggttgg acgaatcgtg ctgggctacc agctggtttt tggttttcag gtagattcgg
     2521 cagagcgctt cgtagcggtc gaaatgctct ttttttgtgt cggctccaga ggtggccgcg
     2581 tgggcgatga ggtgcgcccg gctgaggtag acttccgccg ccgcaaaaca ttcatcggtg
     2641 gctcgcccct tccccgaacc ggcctgttcg aggtagaggt ggcccaagtt gagcaacgcc
     2701 tcggcgtagt tgggattgat ttctaaggcc tgccggaagg cctcctcggc ttctttgact
     2761 ttccctccct ccagctggag cagacctaag gcgttggcat agtcagcgta cggctgcagg
     2821 gagagcgcct tccggtacag cgcttcggct tcggcgcatt tgcccagccg gtggcaaacc
     2881 ccggccaggc gaaagtgggc ctcgctcgaa tcgggatcgc gctccagcgc ccagcggaaa
     2941 caggcttcgg cttccgggaa aaacccgagg accagaaacg cctgacccac gctcgtctct
     3001 tctgtcgccg tgccctgttt ctccaggcgc ttggccaggt agaagagcgt cttcaaaaag
     3061 agcacttcgc gctgatggaa agccccgccg ctgtcgtgaa aactgccggc cggatccacg
     3121 ggaaccgaac ggcgcgcccc caccagccgc cggttgcgga acagctggca gatgaacacc
     3181 ccttccgttt cgtcgtactc ggtttgtgct aaaacatcca ccggccccgt gcggaagaat
     3241 tcctcccaca agaccgctgg tgcctcggga ccggccgctg tcgtcaccgt gacgctattt
     3301 tccattccgc tcgtcgctta aaagggaccg ctggcacggt cggtacgacc cgtaggggcg
     3361 tctgcgccgc accgaccgtc tccacaggga gacgaaaagt ttcaaccacg tcgaccgtta
     3421 ccgttccgga tacgtcccgc taattccggg accgcatccg ctggtaacga aatcggctct
     3481 ttttacgcaa aagtttacgg ctggccagca ggcggaacac ctgccaccag gaagcgcctg
     3541 ggtacccaaa tcgcgagcga agctcgcggg caatttcggc gatggaatag aagggatctt
     3601 ctccgaccag actgagcacc gcgtccaccc aggcctgagg ttcgggagtt ctggtcaccg
     3661 gaattgtcac ggaagttccc gcgcgtgctg gactaccgac cggggcgggg cccgccggca
     3721 gacgaaacgc ttcggcaccc gggcgggaaa ccggaagcga aggggctggc ggtgcagccg
     3781 ctttcggttt ctgatccaat ccgaaatcgc ctttggcctt ttcgacgtta tcgaacgcct
     3841 tgaggatgct gtcgaattcc aacagttcga agatttcgta gaccgaaggc accatccgcg
     3901 ccagtttgat gtcacccgcg ttgcgccgga tttcccggat gttggaaatg aaaatccccc
     3961 accccgccga ggagatgtag tccaccccgc ccaggtcgac cacgatgttg aaccgcttct
     4021 gctcgaaaag cgaattcatc accttctcca attcggaagc ggtaagcgta tccaccaccc
     4081 cgtccacccg gacgatggcc acctcgttct gggggccggc ggtctctaaa gagatgcgga
     4141 tgtcatccat acacttgctc ttggctcagc ggtatcttcg gtatgctccc ctggcagctg
     4201 cggcgctggc tggagccggg ggtgtgccag aatttcccgg gtcaagagga aatagtcaat
     4261 ggccccgtgc gcgtagggac tgtattcgaa gatggtcttt tggaccgcct gcgcctcctt
     4321 gagcgccgta ttgacccgga ttctggtcgc cagcactttt tcgccgtagc actcgagcaa
     4381 ttcgttccac aggcgtttgg cgatgttcgt ccggccgtcg tagaaggtgg ccaaaatgcc
     4441 caagggctcg atgggatgcc ccgcctgccg cacgtc
//
LOCUS       DBNGZA010000418         4227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_829330, whole genome shotgun sequence.
ACCESSION   DBNGZA010000418 DBNGZA010000000
VERSION     DBNGZA010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_829330"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1291)
                     /locus_tag="AC16FU_08960"
     CDS             complement(182..1291)
                     /locus_tag="AC16FU_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994536.1"
                     /translation="MDELDKLFEQNYAQHLLEATQREPKLTAPVDLSRAAQVSVTRDM
                     YVEAQAKGLTLSELLESDEYDPSPLACPLDAFERQLAVKGIKVGGPNPSTVELFFSTA
                     PTLLPEFMLREIKRGQKMRPGLADLGATSTVINANRYTPFFVSGTPTQPKFSLRPIGD
                     GAEVPKLVVAEQLHTITIPDYGMALEVSYKALRHRTTAQFKVLLWYVGFRLQADKMGL
                     VVNTIVNGDGNANPATVLNTAATGVLTYADLITFWAEFFPYELNVVLCHKDKLKSILT
                     LPEFKDPMAGFKFQRTGEVVSPLGSKLIRSDDVPNDLVIGLDSRFAIEEVIAQPVMVE
                     FDKVIKAKLEEAVISESVAYAKVIKEASLVLDTVW"
     gene            complement(1500..1922)
                     /locus_tag="AC16FU_08965"
     CDS             complement(1500..1922)
                     /locus_tag="AC16FU_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994537.1"
                     /translation="MGIRDQNLEGIGAVFATFTIRQTASVDDLKDADVGKAVTITASN
                     QAAPTSDGSILLGKLVDLTLTDADNGKRVATVQIGGVVTLPVVATVPVVGNRVVGGAG
                     GTVKQAPVLAAFDPAGGNIARGTVVAVNGTTDCTLILN"
     gene            complement(2037..2519)
                     /locus_tag="AC16FU_08970"
     CDS             complement(2037..2519)
                     /locus_tag="AC16FU_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994538.1"
                     /translation="MTTTDLIHQLKVHLNLASPDRMTEVDFRQLQRDLDSAVEKLSEM
                     ERTQTELALRARWSDVLAEALVQEMKGKLLALRLTGEASADWPLWQKLIAAAGSPEEF
                     LRLRELIHRKFDSTFPNRLHAQAVGSENGNHKKEIVMEEFKTGSTPRRRHFSAAPLFS
                     "
     gene            complement(2506..2964)
                     /locus_tag="AC16FU_08975"
     CDS             complement(2506..2964)
                     /locus_tag="AC16FU_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994539.1"
                     /translation="MLDNRQPIVKENWLNARGVWLRFLVTFAVTAGGILAAYYSTIGR
                     IDLALAHKADQEQVASLDKKLSTLEVLLTTNAVTREEFHQFRDEVVQRLGRIEARLEP
                     QSRGQTIPGDESSLPFEGRGLRHLSSPYQGEGREGSEFRASSTREYRDDD"
     gene            complement(2954..>4227)
                     /locus_tag="AC16FU_08980"
     CDS             complement(2954..>4227)
                     /locus_tag="AC16FU_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994540.1"
                     /translation="YEKTMNLLGQIQAALAEAGEPVTSDLVALINRRIRPPVPVAIDN
                     VHIRTMFVISDQVNSYGGRLPLEEHPNVARLLVDAPVLIGHAKDRLPVGRNFKAELVE
                     KNGQRWIKVWFYWLKGTAEAESLAQNIDHGIYKECSIGFFFDFPECSVCGKDIRACAH
                     VPLSTFQSADGSEQKVSFNYRGVRKVLETSLVYRGAVPETALSNELFAKAGGNSVGVR
                     FIEPAPVGARRAEPPPKIAPAQKNPNGTFELRRQEKSKKPKTLLILSLETETKVFALE
                     NFDLNRLPTGKRFRCIPIDPKRINGEKNSFALLDTGACKVIGKDELVLTGKVLCGQIS
                     LALAPSQGTFLSQSRVCDTSSEDKGSTTSPDSVQGPVRGVRLEQNAKTTPALVDRSDR
                     SVRTVPEPVKDRVGAAPSPREEGFREQENAR"
BASE COUNT          794 a         1200 c         1266 g          967 t
ORIGIN      
        1 cccaacagta tttcccgctc tacctgaaag agatggagtt ccggtacaac caccgcaaag
       61 agcccatgtt cgaacgattg gcacaactaa tggcaaatcc cgtgtcaaat cttttataat
      121 cacctaaaag atttgtcctt caacttcgac acctcgaccc cccgatactc cgacacagtt
      181 tttaccatac tgtatccagg accagactcg cttctttgat caccttggcg taggcgaccg
      241 attcggagat caccgcttcc tccaacttcg ccttgatcac cttgtcgaac tccaccatta
      301 cgggctgggc gatcacctct tcgatggcaa agcggctgtc cagaccgatg acgagatcgt
      361 tcggcacgtc gtcggaacga atgagtttcg atccgagcgg agagaccact tcgccggtgc
      421 gctggaattt gaagccggcc atcgggtctt tgaactccgg cagggtcaaa atcgatttca
      481 gtttgtcctt gtggcagagg acgacattca gttcgtaggg gaagaattcg gcccagaagg
      541 tgatcaaatc ggcgtaggtc aggacgccgg tggcggcggt gttgagcacc gtcgccgggt
      601 tggcgttgcc gtcgccgttg acgatggtgt tcaccaccag gcccatttta tcggcctgca
      661 gccggaagcc gacgtaccag aggagcactt tgaactgtgc ggtggtgcgg tgccggaggg
      721 ctttgtacga aacttccaag gccatcccgt agtccggaat ggtgatggtg tgcagctgct
      781 cggcaacgac taacttgggc acttccgcgc cgtcaccgat gggcctgagg gagaacttgg
      841 gctgggtcgg tgtgccggag acgaaaaagg gcgtgtagcg gttggcgttg atgacggtag
      901 acgtagcccc gaggtcggcc agtcccggac gcatcttctg cccgcgcttg atttccctga
      961 gcatgaattc cggcaggagg gtcggcgcag tggagaagaa aagttccacc gttgacggat
     1021 tcgggccgcc gactttgatg cctttcaccg ccagttgccg ttcgaaggca tccaggggac
     1081 aggcgagcgg cgagggatcg tactcgtctg attcgaggag ttcggagagg gtcagacctt
     1141 tcgcctgcgc ttctacgtac atgtcgcggg tgacggaaac ctgggcggcg cgcgagagat
     1201 ccaccggcgc agtcaatttc ggttcgcgct gggtggcctc taagagatgc tgcgcgtagt
     1261 tttgttcgaa gagcttatcg agttcgtcca tttcttttcc tttcagttca cttctggggc
     1321 acggcacgcc gtgtccgtac gggttgtctt ccgtctcagt tcatttctgg gcacggcacg
     1381 ccgtgcccgt acgtttacct aagcacagtg ccggagtttt tttgattcgc cattgtcttt
     1441 ccctaccacc taccacccat cacctatccc tcgactttgc tcggggcaag ccacctttct
     1501 taattgagga tgagggtaca gtcggtggtg ccgttgaccg cgaccaccgt gccgcgggcg
     1561 atgttgccgc cggcgggatc aaaggcggcc aggacggggg cttgcttgac tgtcccaccg
     1621 gcgccgccca cgacgcgatt gcccaccacc ggcactgttg ccacaaccgg cagggtcaca
     1681 actccgccaa tctgcacggt ggcgactctt ttcccgttgt ccgcgtcggt caaagtcaaa
     1741 tcgacgagtt tgcccagaag gatcgagccg tcgctggtcg gtgcggcctg attcgaggcg
     1801 gtgatggtga cggctttgcc gacgtcggcg tctttcaaat catccacgct ggcggtctgg
     1861 cggatggtga aggtggcgaa gacggcgccg atgccttcta agttctggtc acggatgccc
     1921 attgcttgct cctttggttt gcgttttcaa atttcctttc tgcggggcaa gatgccccgc
     1981 ctatggagaa tgggggagac ctcaccccca tacgggtcgt accaaccagc cctctcctac
     2041 gagaagaggg gagcggcact gaagtgccgc ctacgcggtg ttgagccggt tttgaattcc
     2101 tccatcacaa tttctttctt gtgattcccg ttctcgctac ccacggcctg cgcgtgcagc
     2161 cggttcggga atgtgctgtc gaacttccgg tggatcagtt cccgcaggcg caggaattcc
     2221 tccgggcttc cggcggcggc aatcaacttc tgccacaggg gccaatcggc ggaggcttcg
     2281 ccggtcaagc gcagggccaa gagtttgccc ttcatttcct gcaccagcgc ttcggcgaga
     2341 acgtcgctcc accgggcgcg cagagcgagt tcggtttgag ttcgctccat ttcagagagc
     2401 ttttcgacgg cggagtcgag gtctctttgt aattgccgga aatcgacttc agtcattcgg
     2461 tcgggtgagg ccaaattcag atgcactttc agctggtgaa tgaggtcagt cgtcgtcacg
     2521 gtattccctc gtggaggagg cacgaaattc tgacccctcc ctaccctccc cttggtaagg
     2581 ggaggaaagg tgtcggagac ccctcccctc aaaggggagg gaggattcat ctccggggat
     2641 ggtttgccct ctgctttgcg gttcgagccg agcctcgatt cggcccaggc gctgaaccac
     2701 ctcgtcacgg aactggtgga actcttctct ggtcacagca ttcgtggtca agagcacttc
     2761 aagggtggaa agtttcttat cgagacttgc gacctgctcc tgatcggctt tgtgggccag
     2821 agcgaggtcg atacgaccga tggtcgagta gtaggccgcg aggatgccgc cggcggtgac
     2881 ggcgaaggtc acgaggaacc ggagccagac gccacgggcg ttcagccaat tttctttgac
     2941 gataggttgg cgattatcga gcattttcct gttccctaaa cccttcttcg cgcggcgagg
     3001 gcgccgcgcc cacgcgatct ttgaccggct cagggacggt ccgtacggat ctgtccgacc
     3061 ggtctaccag agcaggtgtt gtttttgcgt tctgttccag gcggactccc cggaccgggc
     3121 cttgaacaga gtccggggag gtggtgctcc ctttatcctc agaggaggtg tcgcagactc
     3181 gactctgcga gaggaaggtg ccctgggagg gtgccagagc gagggagatt tggccgcata
     3241 aaacttttcc ggtaagcacc aattcgtctt taccaatcac tttacacgcg ccggtgtcga
     3301 gaagcgcaaa cgaatttttc tctccattga ttcgcttcgg atcaatgggt atacagcgga
     3361 agcgcttgcc ggttggaaga cgattgaggt caaaattttc cagagcaaag actttcgtct
     3421 ccgtctccag cgagagtatt aaaagcgtct tgggcttctt cgatttctct tgtcgccgca
     3481 gttcaaaagt cccattcggg tttttctgcg cgggggcgat cttgggaggg ggctcggcac
     3541 gccgagcccc aacgggcgcg ggttcgatga atcgaacccc tacggaattc ccaccggctt
     3601 tggcgaagag ttcgttcgag agggcggtct ccggcaccgc gccgcggtag accagggagg
     3661 tctccagcac cttccgcacg ccgcggtagt tgaaggagac tttctgttca gagccgtcgg
     3721 cgctctgaaa cgttgataac gggacgtgcg cacaggcgcg gatgtctttg ccgcagaccg
     3781 agcattccgg gaagtcaaag aaaaagccga tggagcattc cttgtagatg ccgtgatcga
     3841 tgttctgagc cagcgattcg gcttccgctg ttcctttgag ccagtagaac cagactttga
     3901 tccacctctg gccgtttttc tccaccaact cggctttgaa attccgcccc accggcagcc
     3961 ggtccttggc gtggccgatc agaaccggcg catcgaccaa aagccgcgcc acgttcggat
     4021 gttcttccaa aggcagacgt ccgccgtagg agttgacctg atcggagatg acgaacatcg
     4081 tgcggatgtg gacgttgtca atcgccaccg ggacgggtgg ccggatgcgc cggttaatga
     4141 gcgccaccag gtcgctcgtt accggttcgc ctgcttccgc taatgctgct tgtatttgac
     4201 cgaggaggtt catcgttttc tcgtaac
//
LOCUS       DBNGZA010000419         6122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_296280, whole genome shotgun sequence.
ACCESSION   DBNGZA010000419 DBNGZA010000000
VERSION     DBNGZA010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_296280"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="AC16FU_08985"
     CDS             complement(<1..122)
                     /locus_tag="AC16FU_08985"
                     /inference="COORDINATES: protein motif:HMM:NF036933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994541.1"
                     /translation="MVTVTPSVTTGAHAAGVPYTISLNQGQTYQLINTDAAPADL"
     gene            115..873
                     /locus_tag="AC16FU_08990"
     CDS             115..873
                     /locus_tag="AC16FU_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994542.1"
                     /translation="MTTVESQIVYSNINQGWRYPVYFSRTVADTKDRAGLDPHLQAEA
                     AVWRIVPQQGTNMADIVRADSFLTQVYRYRRWSDYRVTKDWNAASLVMMFPEMLIDLS
                     TTYQGKGDTTRAVMLLEKTVATFPDYWRPYVFLGMYYTAHGRKAKADSLYAAGEVYLR
                     KVLRRDAGNPLYYQFLAGLLHSSGKKDQAYAVLEEARQQLPESNFIFQTLLTLYYQDN
                     RIKELVALARDWLGRNPEDRTATQVIQQFGPQVK"
     gene            1351..2649
                     /gene="dinB"
                     /locus_tag="AC16FU_08995"
     CDS             1351..2649
                     /gene="dinB"
                     /locus_tag="AC16FU_08995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR2994543.1"
                     /translation="MPEPFVVSPAVGGTEPTILHVDIDAFFAQVEQVLNPKLRGKPVA
                     VGGYPGERGVVACPSYEARKLGLKTAMPLSQAYRIAPQTIFIKGEYAVYQQFSQRFYE
                     ILLHFTPKVEMLSQDEAAADVGGSLRRFGTTRKLAETLKQEIQQELGLTTSVGAATNR
                     YLAKIACEFEKPDGLTIVMPGEEENFLRPLSVEVIPGVGSRTQAALGRLGIHTIGDLL
                     RIPPKSLERLFGKRGREIAAIASPGNRPHYGVDFDIEPEEEQKSISRSTSFLANSSDP
                     TFLLATLYYLLERACLALRRLGKLAWTVSVTLRFADYTFNHQAKRLSRPLSAEALIFP
                     EVKQIFQTLYSGSQPLSLVGVGLTNLIPCARQLSLFSDPACGEQGRTTERVLRLTHAL
                     DAVRTRFGYHTLISGRTFPLRKTYSESSRGFILRTPSLSR"
     gene            complement(2694..3560)
                     /locus_tag="AC16FU_09000"
     CDS             complement(2694..3560)
                     /locus_tag="AC16FU_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HIR2994544.1"
                     /translation="MQQKRRRNDPCYCGSGKKFKHCHSAQQQVDNLRFSGIAGAFLDQ
                     ENRLFIMSESVVVNQLRKEAPKIAEGFDKQFSDQLNQISVEVSRAMSILFFGAVGPTR
                     LSKVQETCLPLLLNASDSSVAALELLRNGHRLQPGILIRNVLESLCAALYLFQLPDEI
                     PLFMKGKLDSAKTVGKAKEAVPILGRLYGYFSNEFAHLGVSHRSFNPLIPFEKKDNSA
                     IVNLAFIRMSVALLYITAELIFLDSISRPRYWRKVATKVYSFSPSEKEWDWLINFLGV
                     SIDKTESALRTS"
     gene            complement(3702..5822)
                     /locus_tag="AC16FU_09005"
     CDS             complement(3702..5822)
                     /locus_tag="AC16FU_09005"
                     /EC_number="3.4.14.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR2994545.1"
                     /translation="MKDGVRRSHLGMFLVLTFGVVAFTGGGSLLFADEGMWTFDNPPL
                     KQLQERYRFTPTQEWFDHVRLSSVRFNDGGSGSFVSPSGLVLTNHHVALGQLQKVSSP
                     GKDYVKEGFYAKTQAEEMRCPDLELNVLISMKDVTERVQGVVKKRMSDKEALEARKAE
                     RAKIEKESLDSTGLRSDVISFYQGGEYWLYRYKKYTDIRLVFAPEQQIAFYGGDPDNF
                     TYPRYDLDMALFRVYENDKPVGSRYYLKWNAKGAAEGELVFVSGHPGSTSRLRTLAQI
                     EGSRDYIYPLLIQTLKRRLEVLKRYAERGPEPAREAAGQIFGIENSLKALAGEYNGLL
                     DKNLMAKKKREEEDFRALVASNPDWKKEYGRAWEAIAKAEKKQQKTLTQRWFRGLRGS
                     RLPDLALTIVRYVTEVTKPDGKRFQEYRDSQLESMRFRLFSPAPIYPQLEEALLTHGF
                     QESLDALGPDDPFIKALLQGRAPAEVAKEVITRTKLADPDFRRSLVDGGEAAVAAATD
                     PLIVLARRVDPLLREERKWYEDNIESVETAAGEKIGTARFTVYGKSVYPDATFTLRLA
                     YGTVKGYPMNGTQAPSKTTLYGLYDRAKSFDLRSPYDLPARYVEGTDKLDLATPLNFV
                     STCDIIGGNSGSPVINRNAEFVGVIFDGNIESLIGDLVYHEENNRAVAVHSAAMIEAL
                     RKLYDAAPLADELEGKAYGASGSE"
     gene            complement(5916..>6122)
                     /locus_tag="AC16FU_09010"
     CDS             complement(5916..>6122)
                     /locus_tag="AC16FU_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR2994546.1"
                     /translation="TIGDNVIVAAKSGIHRDLKPGTVYFGYPARELSKQKRIEAVISR
                     LPELAERVKALEKKVKELTGQVQK"
BASE COUNT         1272 a         1841 c         1587 g         1422 t
ORIGIN      
        1 aggtcggcgg gggcggcgtc cgtattgatc agctggtacg tctgtccctg gttcaacgag
       61 atggtgtacg ggactccggc cgcatgggcg ccggtggtga cggacggcgt gacggtgacc
      121 accgtcgagt ctcagatagt ctacagcaac attaaccaag gctggcgcta tccggtctac
      181 ttttcccgca ccgtggccga taccaaagac cgcgccggtc tcgaccctca cctgcaggct
      241 gaagctgccg tctggcggat cgtgccccag caggggacga atatggccga tatcgtacgc
      301 gccgattcct tcctgacgca ggtctaccgc taccggcgct ggtccgacta tcgggtcacc
      361 aaggactgga acgccgccag tttggtcatg atgttcccgg aaatgctgat cgatctctcc
      421 accacgtacc agggcaaagg cgacacgacc cgggcggtca tgctgctcga aaaaaccgtg
      481 gccacttttc cagactactg gcggccttac gtttttctgg gaatgtacta caccgcccac
      541 ggccggaagg ccaaagccga ttccctctac gccgccggtg aagtttacct gcggaaggtg
      601 ctccgccgtg acgcgggaaa tcctctctat tatcaattct tagctggcct cctccatagt
      661 tccggaaaaa aggaccaggc ctacgccgtt ttggaggaag cccgccagca gttgccggag
      721 tcgaacttca tcttccagac tctgctaact ctgtactacc aggataaccg catcaaagaa
      781 ttagtggcct tagcccgcga ctggctgggg cgcaatccgg aagaccgcac cgccactcag
      841 gttatccagc agttcggccc gcaagttaaa taggaacttc ttcacacaga agaatctctg
      901 taggggcgaa tctttacttg tcctgagcga agccgaagga gattcgcccg tttttctcgg
      961 tggatgttgg atttcaagaa ccggccttaa ggccggttct tttttttaca acctcttttc
     1021 gccgcgcgcg gcagatgtaa acatctgccg ccgctcctca aaagtattcc gtagccgagg
     1081 gttttattct cagccccgcc ttcggcgggg tggcattaca aagaggcaac ggctcggtag
     1141 ccgccgaggt tagaaacctc ggctacggct ttggaatcgc cagacccgac cttacttgtg
     1201 gtgagcgagt tgtcttcttc attctcgcgc aggcgggtcg gaaccccgcg taggcggggc
     1261 tcttagcccc gccttctctc tcccttgact actgctcaaa cgtacagtat atttctcccg
     1321 tagcatctga cgccgaaaga gcggatcttt atgcccgaac catttgtggt gagtcccgcc
     1381 gttggcggga ccgaaccaac cattctgcac gtcgacatcg acgccttctt cgctcaggtg
     1441 gagcaggtgc tgaacccgaa actgcggggg aaaccggtgg cggtgggcgg ctatcccggc
     1501 gagcgcgggg tggtggcctg ccccagttac gaagcgcgga agttgggcct caaaaccgcc
     1561 atgcccctct cccaggccta tcgcattgct ccgcagacga tcttcatcaa aggcgagtat
     1621 gccgtttacc agcagttttc ccagaggttc tacgagattc tcctgcactt cacgccgaaa
     1681 gtcgaaatgc tctcccagga cgaagccgcc gccgatgtcg gtggctccct ccggcgtttt
     1741 gggacaaccc gcaagttagc ggaaacgctc aagcaagaga tacagcagga actgggactg
     1801 accacttccg tcggtgcggc gaccaaccgc tacttagcca agattgcctg tgaattcgag
     1861 aaaccggacg ggctgacgat tgtaatgccc ggagaagaag aaaatttctt acgccccctc
     1921 tcagtggaag tcatccccgg cgtcggctcg cgcactcagg ccgcattggg tcgtctgggg
     1981 attcacacga taggcgacct gctccggata ccgcccaaaa gtttggagcg gctcttcggc
     2041 aagcgtggcc gggagattgc agcaattgct tcgcccggca acagacctca ctatggagtg
     2101 gattttgata ttgagccgga ggaagagcag aagtcaattt cccgcagcac cagttttctg
     2161 gccaattctt ccgaccccac cttccttctg gcgacgctct attacctgct ggagcgggcc
     2221 tgcctcgccc tgcgccgatt ggggaagttg gcctggacgg tttcggtgac tctccgcttt
     2281 gccgactaca ccttcaacca tcaggccaaa cggctctcgc gtcccctctc cgccgaagcc
     2341 ctgatctttc cggaggtcaa gcaaatcttt cagacactat acagtggcag ccaacccctt
     2401 tcgctggtgg gagtaggatt gacgaacctc atcccctgcg cccgccaact ctcgctcttt
     2461 tccgaccctg cttgtggtga gcaaggtcga accacggagc gagtcctgcg cctcacccac
     2521 gcgttagacg ccgtccgcac ccgctttggc taccatacac tgatttcagg ccgcaccttt
     2581 cctctgagaa aaacttatag cgagagttcg cgtgggttta tcctgcgtac gccgtcgctc
     2641 tcacggtaac gaccacggcc cactctgatc actgcacccc atctcacgta tatttatgaa
     2701 gttcgtagcg cgctttccgt tttatcaatg gagacaccga ggaaatttat tagccaatcc
     2761 cattctttct cgctcgggct aaacgagtaa acctttgtag ctacctttcg ccagtatctt
     2821 ggcctcgaga ttgaatccag gaaaataagt tctgctgtaa tgtataagag agctacgctc
     2881 atccgaatga aagcaagatt tacgatcgct gagttatctt ttttctcgaa aggaatcaac
     2941 ggattaaaag atctatggga aacgcccaag tgtgcaaact cgttgctaaa gtaaccgtag
     3001 aggcgaccta atatcggaac cgcttctttc gcttttccaa cggtcttcgc tgagtcgagt
     3061 ttgcctttca taaaaagtgg aatttcgtca gggagttgaa agagatacaa tgctgcacag
     3121 agagattcaa gaacattgcg aatcaatatg cccggctgaa gccgatgacc atttcgaagc
     3181 aactcaagag cggcaacaga tgaatcactt gcgttcaata aaaggggaag acacgtttct
     3241 tgcactttag ataaacgggt agggccaacc gcaccaaaaa aaagaattga cattgctcga
     3301 ctcacctcta ccgatatttg gttcaactgg tctgaaaatt gtttatcaaa accttcagca
     3361 attttaggag cctccttgcg cagctggttg acgacgactg actcactcat gataaacaat
     3421 ctattttctt gatctaggaa agccccagct attccagaaa atcttaagtt atcaacctgt
     3481 tgctgcgcac tgtggcaatg cttaaacttt ttgccactac cacaataaca agggtcgtta
     3541 cgacgacgtt tctgctgcac gtatctagct ttgtttactc caaaatttcc taaccctgct
     3601 tatttactcg ccctactaac ccctcgatcc tcttctccag ctccttcacc ctctccgcca
     3661 actccggcag gcgagaaatc accgcctctc acctccggca tttattccga cccgctagcg
     3721 ccgtacgctt tcccctccaa ttcatccgcc agcggagcgg cgtcgtacaa tttgcgcaag
     3781 gcctcgatca tcgcggcgct atgcacggcc acggcgcggt tattctcttc gtggtagacc
     3841 aggtcgccaa tcaaagattc gatgttgccg tcaaaaatca cccccacgaa ctcagcattc
     3901 cggttgatca ccggcgaccc ggaattgccg ccgatgatgt cacaggtgct gacgaaattc
     3961 aaaggcgtgg cgaggtcgag tttgtccgtg ccctccacgt atcgggccgg caggtcgtac
     4021 ggcgaccgca ggtcaaaact cttcgcccgg tcgtagagac cgtacagggt ggtttttgag
     4081 ggggcttgag tgccattcat gggataccct ttgaccgtgc cgtaggccag ccgcaaggtg
     4141 aaggtggcgt ccgggtacac cgacttcccg tacaccgtaa agcgcgccgt gccgattttc
     4201 tccccggctg cagtttccac actttcaatg ttgtcctcgt accatttgcg ctcttcgcgc
     4261 aacagcggat caacccggcg ggccagaacg atcaaaggat cggtggcagc cgctaccgcc
     4321 gcctcgccac cgtccactaa ggacctgcgg aaatcaggat cggccagctt cgttctggtg
     4381 atgacttcct tggcaacctc tgccggagcg cggccctgca gcagggcttt gataaaagga
     4441 tcatccggcc cgagcgcgtc cagcgattcc tggaagccat gcgtgagaag ggcctcttct
     4501 aattgcggat agatcggtgc tggtgagaag aggcggaagc gcatcgattc aagttgcgaa
     4561 tcgcggtact cctggaatcg cttgccgtct ggcttggtca cttcggttac gtaccggaca
     4621 atggtcagtg ccagatccgg caggcgggag ccgcgcagtc cgcggaacca cctctgggtg
     4681 agcgtctttt gttgcttttt ctccgccttg gcgatggcct cccaggcccg accgtattcc
     4741 ttcttccagt ccgggttgct ggccaccagt gcacgaaaat cttcttcctc ccttttcttt
     4801 ttggccatca ggtttttatc cagaaggcca ttgtactccc cggccaacgc cttaaggctg
     4861 ttttcaatgc cgaagatttg tccagccgcc tcgcgcgccg gctccggtcc cctttcggcg
     4921 tagcgtttca acacctctaa ccgtcgtttc agagtttgga tgagcaatgg atagatatag
     4981 tcgcgtgacc cttcgatctg cgccagcgtg cgcaagcggc tggtggagcc ggggtggccc
     5041 gaaacaaaca cgagctcgcc ctcggccgca cctttggcgt tccatttcag atagtaccgg
     5101 ctcccgaccg gcttatcgtt ttcatagacg cggaagagcg ccatatccag atcgtagcgg
     5161 gggtaggtga agttatccgg atcgccgccg tagaaagcga tctgctgctc cggggcaaag
     5221 accagccgga tgtcggtgta ctttttgtag cggtacagcc agtactcgcc gccctggtag
     5281 aaggagatca cgtcggagcg caggccggta gagtctaaac tttctttctc gattttcgcc
     5341 cgctcggctt tgcgcgcttc cagcgcctct ttgtcgctca tccttttttt cacgaccccc
     5401 tgcactcgtt cggtgacgtc tttcatggag ataagcacgt tcagttcgag gtcgggacat
     5461 ctcatctcct cggcctgcgt tttggcgtag aatccctcct tcacataatc ctttccgggg
     5521 gaggaaactt tctgcaactg gccgagggcc acgtggtggt tggtgagcac cagtccgctg
     5581 gggctgacga acgagccgga gccgccgtcg ttgaaacgca cgctcgaaag ccgcacgtgg
     5641 tcgaaccact cctgtgtcgg ggtgaagcgg taacgctcct ggagttgttt cagcggcgga
     5701 ttgtcaaagg tccacatccc ctcatcagcg aaaaggagcg acccgccgcc ggtgaaggcc
     5761 accacgccaa acgtcaaaac gaggaacatc cccagatgtg atcgccgaac accatctttc
     5821 ataagccacc tcccttagtg caaccgttct ggaattgttg ccacattgtt ctgtctttct
     5881 ttgccgcctt caccttaagg cacgaccccc gtcccttact tttgaacttg cccggtcaac
     5941 tctttcacct tcttttccag cgccttcacc ctttccgcca actccggcag gcgcgatatc
     6001 accgcttcga tccgtttttg cttgctcaat tcccgcgccg ggtagccaaa gtaaaccgtg
     6061 cccggcttca ggtctctatg gatgcccgac ttggccgcca cgatcacgtt gtccccaatg
     6121 gt
//
LOCUS       DBNGZA010000420         4624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_829442, whole genome shotgun sequence.
ACCESSION   DBNGZA010000420 DBNGZA010000000
VERSION     DBNGZA010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_829442"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1325
                     /locus_tag="AC16FU_09015"
     CDS             267..1325
                     /locus_tag="AC16FU_09015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2994547.1"
                     /translation="MHLEENFRRIARLGYRVTLLTSQYPGGKREEEVEGLRILRRGHE
                     YTFNFSVPGAVRRLLRAEPFDLVVEDINKIPFYTPWFHHKPLLAIVPHLFADSVFREI
                     NFILGTYIFLAERPIPFIYRGRRFVVISESTKADLIRRGIPEDNITVSCCGIDHTLYS
                     PDKKVAKSGQPTMLYLGRLKKYKSVDHLIRALPHVQVKVPDAQVVIIGEGDDRPRLEK
                     ITRSLGLVANVQFLGFVAPDEKVNWLRRAQVAVCPSLKEGWGLTNIEANACGTPAVAA
                     DVPGLRDSVSDGTSGLLYPYGDVAQLAERLIRILTDVRLRGNLERGALAWARKFQWDT
                     AARELAGVIDEVVGRERR"
     gene            1322..2344
                     /locus_tag="AC16FU_09020"
     CDS             1322..2344
                     /locus_tag="AC16FU_09020"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2994548.1"
                     /translation="MTKRLRWQFLIGIGISVVFLYLAFRGVKLSELKRALAAVNYWYL
                     LPSIALILASFVVRAYRWGWMVAPIKPARFGNLFAATLIGFMANNVLPFRLGEIGRAY
                     SLGHLEKISRSSSLATIILERFFDTVGLLLLTIVILIWFPFPPHLRWIPPMAVTVTAV
                     ALLFLVFLKLKGERAGEILAHLLRVLPGQFGQKTGEIVTKFSQGLGLLSRPAAVLLVL
                     FHSLVIYCILAAAGYVVLLAFGLNPGILAPFVLLVVVSLGLILPAAPGFVGTFQQLTI
                     WALAPFGINKSTALSFSIVLHASHYFPVTLLGLYYLRKKHLSLAAVEKLASGPDPVGP
                     LPGKEN"
     gene            2432..3511
                     /locus_tag="AC16FU_09025"
     CDS             2432..3511
                     /locus_tag="AC16FU_09025"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR2994549.1"
                     /translation="MLYAIIIAGGRGERFWPLSRSGHPKQLLRLLSEKTLLQETIDRV
                     QCFIPIDRTVVVTGENMQKAVLGAVPYLREKHILAEPEGRNTCLAIGLAAVHLVKRDP
                     DAVMVVLSSDHAVQPAERLVEILKTAAQVASSGPYLLTLGVVPTRPETGYGYIELGER
                     HAQQDGIEVFRVGQFKEKPSRTVAQEYYFDRKHLWNSGMFVWTARTFLDAVRQHLPEL
                     AGLLEAYAPAIGTPKEKQARLVLYQSAESISVDCAILEKAKNVLTIKSDFKWDDVGSW
                     LALERVKPKDRENNVILGDSLLVDSYETTLVNDGEGLVVTYGVSDLVIVKTNQIVFVA
                     HKTKVGDLKQLVAKLNQDRKAQAYL"
     gene            3521..4306
                     /locus_tag="AC16FU_09030"
     CDS             3521..4306
                     /locus_tag="AC16FU_09030"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR2994550.1"
                     /translation="MSLRPLRVVNPHRPSAFSIIRRPPLATPTLPLADCIQPSPPLSQ
                     ADRPRTTRWLRVRGGRVTAAQLRIAVDTLAQGGIVVLPTDTVYGLAADALNRKAVSRL
                     AQVKGRTAKQPVAILLGSAKEMKTWGKIFPTVKRLSETFLPGPFTLVVPTTFQTPRHL
                     VQNGQIGLRVPKSRLAQKLVVCLGRPLAATSANRMGQPPCRTGREAYRVFRGAVDLVL
                     DAGVKSGPASTVVQVVKGKLRVIREGPVSIRTLQQAGFRVGQP"
     gene            4312..>4624
                     /locus_tag="AC16FU_09035"
     CDS             4312..>4624
                     /locus_tag="AC16FU_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR2994551.1"
                     /translation="MFTVLFVCTGNTCRSPMARGIFRQLLTEQGAGDIELLSAGMAAV
                     EGLPATELATATARRWRIDISSHQSRRLDPELVARADLILAMSDEHVEAILALDPEAA
                     ER"
BASE COUNT          910 a         1360 c         1400 g          954 t
ORIGIN      
        1 tacgccgacg cgcgtacggt gatcgaacag tgggtatccc gtcacccgga acaggcggaa
       61 tatctggcgg cgttcaaccg ggccgtgaat caggataccg cccgcaaggt cgagccgccg
      121 aacaaaaaac cggacactgc ccgcgcaggc ctgcagccca aagtccgatc caactgaacc
      181 cttcgagaag cgagcgcact ctggggtacc ggattttagt catcaactgg cgcgacctca
      241 aaaatccacg cgcggggggg gcggaggtgc acctcgaaga aaatttccgc cggattgccc
      301 ggctggggta ccgggtgacg cttttaactt cgcagtatcc cggcgggaaa agggaggagg
      361 aagttgaagg gctccgcatt ctgcggcggg gccacgaata caccttcaac ttctcagtgc
      421 cgggagccgt gcgccggctc ctgcgcgccg aaccattcga tctggttgtc gaggacatca
      481 acaaaatccc tttttatacg ccctggtttc accataagcc gcttttggcc atcgtacccc
      541 acctgttcgc agattcagtc tttcgggaaa tcaacttcat tctggggacc tacatttttt
      601 tggccgagcg acctattcca ttcatttacc ggggccgccg gttcgtggtg atttccgaaa
      661 gcaccaaggc cgatttgatc cggcgcggta ttccggaaga caacattacg gtgagctgct
      721 gcgggattga ccacacgctt tactcgccgg acaaaaaggt cgccaagtcc gggcagccga
      781 cgatgctcta tttggggcgg ttgaagaagt acaaaagcgt cgatcacctg attcgggcac
      841 tgccgcacgt tcaggtgaaa gtacccgacg ctcaggtggt cattatcggg gagggagatg
      901 accggccgcg actggaaaaa attacccgct cgctggggct cgtggccaac gttcagtttt
      961 tgggttttgt ggccccggac gaaaaggtca actggctcag acgggctcag gtggcggtct
     1021 gcccgtcgct caaagaaggg tgggggctca ccaatatcga agcgaacgcc tgcggcacac
     1081 cggcggtcgc ggccgacgtg ccgggcttgc gcgactcagt cagtgacggg accagcggac
     1141 ttttgtatcc gtacggagac gtggcgcaac tggcggagcg gttaatccgg attctcaccg
     1201 acgtgcgcct gcggggtaat ctggaacgcg gcgcactggc gtgggcccgg aagtttcagt
     1261 gggacaccgc ggcgcgtgaa ttagccggag tgatcgatga agtagtgggg agggaaaggc
     1321 gatgacgaaa cggctgcgct ggcagtttct catcgggatc ggaatcagcg tcgtattcct
     1381 ctacctcgcg tttcgcgggg tgaaactttc ggaattgaaa cgggccttag ccgccgtcaa
     1441 ctactggtac ctgttgccaa gcattgccct gattttggcc agctttgtcg tacgggcgta
     1501 ccgctggggg tggatggtcg cacccatcaa accagcgcgc tttggaaacc tcttcgccgc
     1561 cacgctgatc ggctttatgg ccaacaatgt cctgcccttc cggttggggg aaatcggccg
     1621 tgcttactcg ctgggccacc tggagaaaat cagccgcagt tcctctttag ccaccatcat
     1681 tctggaacgt ttcttcgaca cggtcgggct tttgctcttg acgattgtca tcctcatctg
     1741 gtttccattt cccccgcacc tgcgctggat tccgccgatg gcggtcacgg tcaccgccgt
     1801 ggcgcttctg tttttggttt tcctcaaact caaaggtgaa cgtgccggag aaattctggc
     1861 gcacctgctc cgggttttgc ccgggcaatt cggccagaaa accggcgaaa tcgtcacgaa
     1921 gttttcccag gggctggggc tcctgtctcg tccggcggcg gtgcttttgg tcctgttcca
     1981 ttccctggtg atttactgca tcctggcggc tgccggatac gtcgtccttc tggcctttgg
     2041 gctgaacccg ggaatcctgg cgccctttgt gcttttggtg gtcgtctccc tgggtttgat
     2101 tctcccggcg gcgcccggtt tcgtcggcac gttccagcag ctcaccatct gggctctggc
     2161 gccgttcggc atcaacaaga gcactgcgct gtcgttttcc atcgttttac acgccagcca
     2221 ctacttccca gtgacgcttt tgggtctgta ctacctgcgg aaaaaacacc tttcgctcgc
     2281 ggcagtggaa aagctggcca gcgggcccga cccggtcggc cccctccccg gaaaagaaaa
     2341 ttaaagagca ccaacgaaca ggcaaaatcc aagttcttga cttgcttcgg attgcctctt
     2401 aagtttgtgg tgtgaaaagg agcaggtgac catgctctac gccattataa tagccggggg
     2461 acgaggggaa cgcttctggc cgctctcgcg ctcaggccac cccaagcagc tcttgcgttt
     2521 gctctccgag aaaacgctcc tgcaggagac catcgaccgg gtgcaatgtt ttattccgat
     2581 agaccggacg gtggtcgtca ccggcgaaaa tatgcaaaaa gcagttctgg gggcggtgcc
     2641 ctacttgagg gaaaaacata ttctggcgga gccggaagga cgcaacacct gtttggccat
     2701 tgggctggcg gcggtgcact tggtcaaacg tgacccggac gcagtgatgg tggtgctctc
     2761 ctcggaccat gccgttcagc cggccgagcg actggtggaa attctcaaaa ccgcagcaca
     2821 ggtggcctcc tccgggccct acctgctcac cctgggggtc gtgcccaccc ggcccgagac
     2881 cggttacggc tatatcgagt taggtgagcg gcatgcgcag caagatggca ttgaggtttt
     2941 ccgggtcggc cagttcaaag agaaaccctc gcgcacggtg gcccaggagt actacttcga
     3001 ccgcaaacac ctctggaatt ccgggatgtt cgtctggacc gccaggacct ttctggacgc
     3061 tgtccgccag cacctgccgg agctcgccgg gctgctggaa gcgtatgcac cggccatcgg
     3121 cacgccgaag gaaaaacagg ctcggctggt cttgtaccaa tcggcggaga gcatttcggt
     3181 cgactgcgcc attctggaga aagccaaaaa cgttctgacc ataaagtcgg atttcaaatg
     3241 ggacgacgtc gggtcttggc tggccttgga gcgcgtcaag ccgaaagacc gcgaaaacaa
     3301 cgtcattttg ggcgattcgc tgctggtcga ctcgtacgaa accacgctgg tcaatgacgg
     3361 ggaggggctg gtggtcacct acggcgtgtc cgatctcgtg attgtcaaaa ccaaccagat
     3421 cgtcttcgtg gcgcataaaa ccaaagtggg cgatctgaag caacttgtgg ccaaactgaa
     3481 ccaagaccgg aaggcgcaag cctacctgta agcgggagac ttgagtctgc gaccattgcg
     3541 cgtggtcaat ccgcaccgcc cgtctgcttt ttccatcatc cggcgacccc ccttggctac
     3601 gcccacctta cccctggccg actgcattca accaagcccg ccgctttccc aagccgaccg
     3661 gccccgaacg acccgctggc tccgcgtccg cggcgggcgg gtcactgctg cacaattgcg
     3721 cattgccgtg gacacgctgg cgcaaggagg catcgtcgtt ctgccgaccg ataccgttta
     3781 cgggctggcg gccgacgcgc tgaatcgaaa ggcggtgtcg cgcctggcgc aggttaaggg
     3841 aaggacagcc aaacagcccg tggcgattct tttggggtcg gcgaaggaaa tgaagacatg
     3901 gggaaaaata tttcccacag taaagcggct gagcgaaaca tttctccccg ggcctttcac
     3961 ccttgtcgtt cccaccactt ttcagacacc cagacatctg gttcaaaacg gtcagatcgg
     4021 cctgagagtg cccaaatcgc gtttagctca gaaactcgtg gtgtgtttgg gccgacccct
     4081 ggcggctacg agcgccaatc gtatgggcca gccgccctgc agaaccggcc gggaagcata
     4141 ccgggtgttt cgcggcgcag tcgatctcgt acttgatgcg ggagtaaaat cagggccggc
     4201 atccaccgtg gtgcaggttg tcaaggggaa actgcgggtg attcgcgaag gacctgtctc
     4261 gattcgcacc ttgcagcagg ccgggtttcg ggtcgggcaa ccgtagccgg catgtttacg
     4321 gtcctctttg tctgcaccgg caacacctgc cgcagcccaa tggcccgggg gatcttccgc
     4381 cagttattga ccgaacaggg ggccggagat attgagcttc tttcggccgg gatggccgcc
     4441 gtcgaagggt tgcccgctac cgaattggcc acggccaccg cccgccggtg gaggatcgac
     4501 atcagcagcc accagtcgcg gcggttggac ccggagctgg tggcccgggc cgacttgatt
     4561 ctggcgatgt cggacgagca cgtggaagcc atcctggctc tggatccgga agccgcggag
     4621 cgca
//
LOCUS       DBNGZA010000421         9181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1144552, whole genome shotgun sequence.
ACCESSION   DBNGZA010000421 DBNGZA010000000
VERSION     DBNGZA010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1144552"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..735)
                     /locus_tag="AC16FU_09040"
     CDS             complement(148..735)
                     /locus_tag="AC16FU_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994552.1"
                     /translation="MNIYDEFTDDPYADTGCTPYRPGDEITRQLVLVRSRYRDVVERV
                     AIWRESKSEYRKKLMLKDIIVDLFSIDDNIRPLCNRVLSPNFSFSDKIDDTSKKKFGS
                     YYRTYCRLFTLKKKLLRDIRNGIGAHLELRVYDRHSELWNKITDKDLKEILSSIPPLF
                     NFLKDLDVYTWVKVLSERKGEEIIAISQPLSDWQI"
     gene            complement(781..1863)
                     /locus_tag="AC16FU_09045"
     CDS             complement(781..1863)
                     /locus_tag="AC16FU_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR2994553.1"
                     /translation="MQKKILLVTASLLLILAAGIEPQPALPGEKGDPTSQGQLQDPRE
                     KHLKNIRQLTFGGENAEAYFSADGRILSFQRTSETWPCDQIYTMTTTGDSLRLVSTGK
                     GRCTCAYISPDGERIIFASTHLFGDSCPPKPDYSQGYVWSLYKSYEIFSAKPDGSDLV
                     RLTNDNSYDAECVFSPDGKKILFTSDRDGDLELYAMDADGKNPQRLTHSPGYDGGAFY
                     SADGKMICFRAQVIKDSTELRDYQTLLAKGLIRPHTLELFVMNADGTGRKQITQNGSA
                     NFCPFFSPDGKKIIFASNMNDPKGRNFDLYLINIDGTGLERVTYNETFDGFPMFSPDG
                     KKLVFASNRHNARPGETNIFIADWVW"
     gene            complement(1901..2038)
                     /locus_tag="AC16FU_09050"
     CDS             complement(1901..2038)
                     /locus_tag="AC16FU_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994554.1"
                     /translation="MFRARWQRWRQKGWQFALLLVLAYLVRDLFLYVVLPVFALQTVC
                     R"
     gene            2212..3627
                     /gene="hslU"
                     /locus_tag="AC16FU_09055"
     CDS             2212..3627
                     /gene="hslU"
                     /locus_tag="AC16FU_09055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR2994555.1"
                     /translation="MRGAEKVKELTPKEIVKELDKYIIGQDKAKRSVAIAMRNRWRRQ
                     QLGEELRQEVMPNNILMIGPTGVGKTEIARRLAALANAPFIKVEASKFTEVGYVGRDV
                     ESMVRDLVDLSVNTVKREQQAKVQQKAQQLAEDRVLDLLLPGDHKRKPVSTPVAVGTG
                     ETLVEMTPEPDLASDNDTWEQTREKLRGQLRDGKLEKRLVEIEVPNHAFPVIEIFSPM
                     GMEELGINFQEMFSGMMPKKTKRRRMSVGEAQRFLVQEEAQKLIDMDEVIGLALDRVE
                     NSGIIFVDEIDKIAGEKSKEGPDVSREGVQRDILPIVEGSNVMTKYGMVKTDHVLFIA
                     AGAFHVSKPSDLIPELQGRFPIRVELDSLTTKDFVRILTEPKNALIKQYTALLATEGV
                     EVSFSKDAIQEIAAIANQVNERTENIGARRLHTVMTTLLDNLLFEASELKGKKVKLTR
                     ESVRKALKEIVEDEDLSRYIL"
     gene            4123..>9181
                     /locus_tag="AC16FU_09060"
     CDS             4123..>9181
                     /locus_tag="AC16FU_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR2994556.1"
                     /translation="MSNSTLAATVVTDRPDYAPGETVVITGSGFAASESVTVRVTHVD
                     TTIIGGAGHDPWTVKASPAGGFTTYWVVPFDDNVGQTLKVTATGKTSGLVATTTFNDV
                     NSKITITSIPSTVVAGDTFMVTARLVQNCGGAVDSPLVNRTVLFFLTEGNCGNDVGQV
                     APDSDQTDANGFATGKLVIPFAGSWGVRVKYRGEDKPDPCPTPGNSACDPFDPAANKR
                     CVDISSSNDCKGIQAIACTPPVITCPNDTTVCNGTGQCSRIVNYPSPTTTAGCGPVTV
                     ACTPASGSAFPVGMTTVTCIASDTVGKKDTCTFKVTVNDCQKPTITCPANIVKPNDPG
                     QCCAVTTFAPTVSDNCPGVTFVCTPASGFCFPVGVTTVKCIVTDAASNKDSCTFTVTV
                     NDTQKPTITCPAPIVKPNDPGQCCAVTTFAPTFSDNCPGATVVCTPASGFCFPVGVTT
                     VKCIVTDASSNKDSCTFTVTVNDTQKPTITCPAPIVKPNDPGQCCAVATFTPTIGDNC
                     PNPTFVCTPASGFCFPVGVTTVKCIVTDASSNKDSCTFTVTVNDTQKPTIVCPADSVK
                     NADAGQCCAVEVFAPTVNDNCPGATFVCTPASGFCFPVGMTTVKCVATDAAGNKDSCT
                     FKVTVNDNIKPTITCPADIVKNNDPGQCCAVTTFAPTVSDNCPGVTFVCTPASGFCFP
                     VGVTTVKCIVTDAASNKDSCTFTVTVNDTQKPTITCPAPIVKPNDPGQCCAVATFAPT
                     VSDNCPGVTFVCTPASGFCFPVGMTTVKCVATDAAGNKDSCTFKVTVNDNIKPTITCP
                     ADIVKPNDPGQCCVVATFTPTVSDNCPGATFVCTPPSGTCFPVGMTTVKCVVTDAAGN
                     KDSCTFKITVNDTQKPTITCPADIAKSTDPGKCCAVATFSPTINDNCPGATVVCTPAS
                     GFCFPVGMTTVKCVVTDAASNKDSCTFKVTVNNGPPILSFPGGKNRSINLCESICFPV
                     KATDPEGDSVTITKLSAEGGLSCPPQPKPSPDSCEFCFTPTVPGLYRAIFRAADKCGN
                     MTTDTVVITVNNERLFVEGPRDSSLFLCAGDTIKFQVCAHDCDTLTLTKECGPGTFPP
                     ERDRSPVCGTLTFVPPSSGLYTWCFKAVDRFGAMAVDTVRFNVTVNSPPNISVTPSLP
                     ETVTVGDTVCFTVTGTDIDAGDIVTLTVSPPGFQKQCSPPGPNRTCTFCVTSSEPGTF
                     CLVFKAADNCGATALDTVCITFQQCFRFVIDTATAFLGQHSVVLCVNNVVEAFDPSVT
                     PNPSGVGGFNFLFAYDPTCLTFQKAEMGQFLKDCQWEFFTFRFGTRGNCGTCPSGFIR
                     VIGIADINDALQHHPPASCFHRNAGQWICLFFEVTRDRTFQNTCCPVRWAWEDCTDNT
                     VSDSTGNFLWVVDSVYNAHGVPIDLATEYGIGVDVSNCVSSGGPGKPQTIKCIVFKDG
                     KICIPADSSIDARGDINLNGIPNEIADAVLHENCLLYGMSALSSDPLRRQAQIAASDV
                     NNDGLDMTVADLVYQLRIITGDAQPFPKPAGGASPDVTIDFAERGSELVVTSNNPGLD
                     LGGLLLVLQVEGTVENVAATGRAGAMGLRFAQTGDQLRILVDSYERGKIIAAGTGEIL
                     KISGVRSAEFVEGQAANRGGYPVALELAKSPLPTQFSLSQNRPNPFNPTTRITLALPV
                     ASDYTLVIYNVAGQVVRT"
BASE COUNT         2019 a         2522 c         2616 g         2024 t
ORIGIN      
        1 ctaccagttg gcgaccgggg gcggccatac ggcccgtagg ggtcgccctg cgcataaggg
       61 cctatacccc gcgtaggcgc ggcgcctcgc cgcgcaagtg cataaagccc ggtcctacca
      121 taccaaatcg gtaatgaaaa tgtttgtcta aatttgccag tcagaaagag gttggcttat
      181 cgcaattatc tcttcccctt ttctttcgct caacactttt acccaagtat atacatctaa
      241 atctttcaga aaattaaata gcggaggaat agaagaaagg atttccttta aatccttgtc
      301 cgtaatcttg ttccacagtt ccgagtggcg atcatagact ctcagttcga gatgagctcc
      361 aataccattt cgtatgtccc tgagcaactt tttcttcagg gtaaataagc ggcaataggt
      421 acggtaataa gatccaaact tcttttttga ggtatcgtct attttatcgg aaaaagaaaa
      481 atttggtgaa agaacacggt tgcataaagg tcgaatattg tcatcgattg aaaataagtc
      541 tacaatgata tctttgagca ttagtttctt tctatattca ctcttcgatt ctctccagat
      601 tgcaactcgc tcaacaacat ctctgtacct gcttctcact aaaactaatt gccgagtgat
      661 ttcgtctcca gggcggtagg gagtacatcc cgtatctgca tagggatcat cggtaaattc
      721 atcgtaaatg ttcatggaaa aaggatacca aagatattct taaagatcaa gaattctttt
      781 tcaccacacc cagtcggcaa tgaaaatatt cgtctccccc ggcctggcat tatgccggtt
      841 ggaggcgaag actaacttct tgccgtcagg cgaaaacatc gggaagccgt caaacgtctc
      901 gttgtaggtg acccgctcca gacccgtgcc gtcgatgttt atcaagtata agtcaaagtt
      961 gcggcctttg ggatcgttca tatttgacgc gaaaatgatt ttcttcccat ctggcgaaaa
     1021 gaaggggcag aagttcgccg agccgttctg ggtgatttgt ttccggcccg tgccgtcggc
     1081 gttcattacg aacagttcca gtgtgtgcgg gcggatcaaa cctttagcca aaagcgtctg
     1141 gtagtcgcgc agttcagttg agtctttgat cacctgcgcc cggaagcaga tcattttgcc
     1201 gtcggctgag taaaaagcgc cgccgtcgta gccgggagaa tgagtcaggc gctggggatt
     1261 tttgccgtcg gcatccattg cgtacagctc aaggtcacca tcccggtcgg aggtgaaaag
     1321 aattttcttc ccgtctgggg agaagacgca ctcggcgtcg taactattgt cattcgttaa
     1381 acgcaccaga tcagagccat cgggtttggc agagaagatt tcataacttt tgtaaagcga
     1441 ccagacgtac ccctgcgagt agtcgggctt gggcgggcag gagtcgccga agaggtgggt
     1501 cgaggcgaag atgattcgtt cgccatccgg cgaaatgtag gcgcaggtgc agcgcccctt
     1561 gccggtggag accagccgga gcgagtcgcc tgtggtggtc atggtgtaaa tctgatcgca
     1621 gggccaggtc tcgctggtcc gctggaaact caggatgcgt ccgtcggcgg agaagtaggc
     1681 ctcggcgttt tcaccgccga aggtgagctg ccggatgttc ttcaggtgct tctcgcgggg
     1741 gtcttgcagt tgaccctgtg acgtcgggtc gcctttttct ccgggcaagg cgggttgtgg
     1801 ttcaataccg gctgccaaga tgagcagaag ggacgcagtt accagcagaa ttttcttttg
     1861 cattgggttt gtccttttcg ttagggtcat tgcgtagtcg ctaccgacag acggtttgca
     1921 aagcaaagac cgggagcacc acgtacagga aaaggtcgcg gacgaggtaa gccagcacga
     1981 gcagcaaggc gaactgccag cccttctggc gccagcgctg ccagcgggcg cggaacattt
     2041 ttggcgcagg ttccgtgggc gcaggaattt cccggggagc ggatttcctc cagattgcga
     2101 acttcactca aagccaaata cgagaaccag gtcaagaaaa gatatgacgc agatcatctc
     2161 cgcggttcgc cattggactg gttgcggtta cgtattatta tggttacgct aatgagagga
     2221 gcagaaaaag tgaaagaatt aacccccaaa gagatcgtca aagaattaga taaatacatc
     2281 atcgggcagg ataaagccaa gcgctcggtg gcgattgcca tgcgcaaccg ctggcgcagg
     2341 cagcagttgg gcgaagagct gcgccaggag gtgatgccca ataatattct catgatcggg
     2401 cccaccggcg tgggcaagac cgaaatcgcc cgccggctgg ctgcgctggc caacgcgccc
     2461 tttataaagg tggaggcctc caagttcacc gaagtgggct acgtgggccg ggacgtggag
     2521 tcgatggtcc gggacctggt ggatctatcc gtcaatacgg tcaagcggga acagcaggcc
     2581 aaagttcagc agaaagccca gcagttagcc gaagaccggg ttttggacct ccttttgccg
     2641 ggcgatcata agcgtaagcc ggtgagcaca ccggtcgccg tcgggacggg tgaaactttg
     2701 gttgagatga cccccgagcc ggatctggcg tccgacaacg atacctggga gcagaccagg
     2761 gaaaaattga gggggcagct gcgggacggg aaactcgaaa agcggctggt tgaaattgag
     2821 gtgcccaacc acgcttttcc ggtgattgag attttctccc cgatgggaat ggaagaattg
     2881 gggatcaact tccaggagat gttctccggc atgatgccca aaaagaccaa gaggcggcgg
     2941 atgtcggtgg gcgaagcaca gcgttttctg gttcaggaag aggcccagaa attgatcgat
     3001 atggacgagg tgatcggctt agcactcgac cgggtggaga actcaggtat tatctttgta
     3061 gacgaaattg acaagattgc cggggagaag agtaaggagg gcccggacgt ctcccgggag
     3121 ggggtccagc gggacatcct ccccattgtc gaaggctcga acgtgatgac caaatacggg
     3181 atggtcaaaa ccgaccatgt cctcttcatc gcggccgggg cgttccacgt ctccaagccc
     3241 tccgacctga tccccgaact ccagggccgc ttccccatcc gggtggagtt agacagtctc
     3301 acgaccaaag attttgtccg gattctgacc gagccgaaga atgccctgat aaaacaatac
     3361 actgcccttt tggcaaccga gggggtggag gtgtcgttct ccaaagacgc cattcaggag
     3421 attgccgcaa ttgccaatca ggtcaatgag cggaccgaaa atatcggcgc ccggcggctg
     3481 cacacggtga tgaccacctt gttggataac ctgctttttg aagcctcgga gctgaaaggc
     3541 aaaaaggtga aattgacccg cgaatcggtg cgcaaggcct taaaagagat agtggaagac
     3601 gaagacttga gccggtatat cctttaaaaa tgaccgcaac tttaataggt ttaatgggtt
     3661 agcgggaggg aacatttttt tctttattgt aaaaaagtac ttgacaagtt ttagccaggc
     3721 gttattttat agttgcgttt aggtcaaagc acaaagtaac caaatcccac tgaaaaagcc
     3781 ccacacgggg caacttaata ttttcgccag cattttttag tttacctgcg caccgcaaag
     3841 ttccttggtt ttgcgggcag atgagttgca tcagtgaatt ttgcagtgcg caggcgaggc
     3901 gaaaagggag ttaaccaagc aagcaggaga acaatgaagg tattccgaga tgggttgtag
     3961 gagatttcgg cagagaagtt ggattacgca aaaccagact gatacgaagg taagaacggt
     4021 ttccttgctt ctcctgcggg gggtgctatg cggcaactga gatggggtgg tttgtcttgt
     4081 tctcttttct ttctgatcct gctggggggc tatctaactg gcttgtccaa ttcaaccttg
     4141 gccgccactg tggtgaccga caggcctgat tatgctcctg gcgaaacagt cgtcattacc
     4201 ggcagtggtt ttgccgccag tgaatcagtc acggtccggg tcacccatgt cgacacgacc
     4261 attatcggtg gcgccgggca cgatccctgg acggttaagg cctcgccagc gggtggtttc
     4321 accacttatt gggttgttcc ttttgacgac aatgtcggcc aaaccctgaa ggttaccgca
     4381 accggcaaaa catcgggttt ggtggcaacg acgacattca atgacgttaa tagtaaaatt
     4441 actataactt cgattccatc cacggttgtg gcgggtgaca cctttatggt caccgcccgg
     4501 ctggtgcaaa actgtggcgg cgcagttgac tcgcccttgg ttaaccgtac cgtccttttc
     4561 tttttgaccg agggcaactg cggaaacgat gtcggacagg ttgctccgga cagcgatcag
     4621 accgacgcca atggatttgc caccggcaaa ctggtgattc cctttgctgg ttcctggggc
     4681 gtgcgggtca agtaccgcgg agaggacaaa cctgatccct gtcccacgcc cggcaacagt
     4741 gcctgcgatc cttttgatcc cgctgccaat aagcggtgtg ttgatatttc ctcttcgaac
     4801 gattgcaaag gcatccaggc aatcgcatgt accccccctg tgattacctg tccgaatgac
     4861 actacagttt gtaatggcac tggtcagtgt tccagaatcg ttaattatcc ttctccaacc
     4921 accacggctg ggtgcggccc ggttaccgta gcctgcactc ccgcatctgg tagtgccttt
     4981 ccggtgggaa tgaccactgt tacctgcatt gccagcgata cggtgggcaa aaaagatact
     5041 tgcacattta aggttaccgt caacgactgc cagaagccga ccattacctg tccggccaac
     5101 atcgtgaagc cgaacgatcc aggtcagtgc tgtgcagtaa cgacctttgc tcccaccgtc
     5161 agcgacaact gccccggggt tacctttgtc tgcacgccgg cgtcggggtt ctgcttcccg
     5221 gtgggggtga cgacggtgaa gtgtatcgtc accgacgctg ccagcaataa agattcctgc
     5281 accttcacgg tgacggtgaa tgatacgcag aagccgacga tcacctgtcc ggcgcctatt
     5341 gtgaagccga acgatccggg tcagtgctgt gcagtaacga cctttgctcc gaccttctca
     5401 gacaactgtc cgggcgcgac ggtagtctgc acgccggcgt cggggttctg cttcccggtg
     5461 ggggtgacga cggtgaagtg catcgtcacc gacgcctcca gcaataaaga ttcctgtacc
     5521 ttcacggtga cggtgaacga cacgcagaag ccgacgatca cctgtccggc gcctattgtg
     5581 aagccgaacg atccgggtca gtgctgcgcg gtggcgacct ttactcctac catcggcgac
     5641 aactgcccca acccgacctt tgtctgcacg cccgcttccg gcttttgttt cccggtgggg
     5701 gtgacgacgg tgaagtgcat cgttaccgac gcctccagca ataaggattc ctgcaccttt
     5761 acagtaacgg tcaatgatac tcagaagccg acgatcgttt gtccggccga cagcgtgaag
     5821 aacgctgatg cgggccagtg ctgcgcggtg gaggtctttg ctcccacggt caacgacaac
     5881 tgccccggcg ccacctttgt ctgcacgcct gcttcgggct tctgcttccc ggtggggatg
     5941 acgacggtga agtgtgtggc taccgatgcg gcggggaaca aggacagctg cacgtttaag
     6001 gtcaccgtaa atgacaatat aaagccgacc attacctgtc cggctgacat tgtaaagaac
     6061 aacgatccag gtcagtgctg tgcagtaacg acctttgctc ccaccgtcag cgacaactgc
     6121 cccggagtta cctttgtctg cacgccggcg tcgggcttct gtttcccggt gggggtgacg
     6181 acggtgaagt gcatcgtcac cgacgctgcc agcaataaag attcctgtac cttcacggtg
     6241 acggtgaacg acacgcagaa gccgacgatc acctgtccgg cgcctattgt gaagccgaac
     6301 gatccgggtc agtgctgcgc ggtggcgacc tttgctccca ccgtcagcga caactgcccc
     6361 ggggttacct ttgtctgcac gccggcttcg gggttctgct tcccggtggg gatgacgacg
     6421 gtgaagtgcg tggccaccga tgcggcgggg aacaaggaca gctgcacgtt taaggtcacc
     6481 gtaaatgaca atataaagcc gaccattacc tgtccggctg acatcgtgaa gccgaacgat
     6541 ccaggtcaat gctgtgtggt agcaaccttt actcccaccg tttcggacaa ctgtcccggc
     6601 gccacctttg tctgcacccc gccgtccggc acctgcttcc cggtggggat gacgacggtg
     6661 aagtgtgtgg tcaccgacgc tgccgggaat aaggacagct gcactttcaa gataacggtg
     6721 aacgacacgc agaagccgac gatcacctgt ccggcggata ttgcgaagag caccgacccc
     6781 ggcaaatgct gtgcggtggc gaccttcagc ccgacgatca atgacaactg tccgggggcg
     6841 acggtggtct gcacgccggc ttcggggttc tgcttcccgg tggggatgac gacggtgaag
     6901 tgtgtggtga ccgatgcggc cagcaacaaa gacagctgta cgttcaaggt aacggtcaac
     6961 aacggcccgc cgattttgag cttccctggg ggcaagaacc ggtccatcaa cctctgtgaa
     7021 agcatctgct tcccggtgaa ggccaccgac ccggaaggcg attcggttac catcaccaag
     7081 ctttcggctg agggagggct ctcctgtccg ccacagccga aaccctcgcc cgattcctgt
     7141 gagttctgct ttacgcccac cgtgccgggg ctctaccggg ctattttcag ggcggccgat
     7201 aagtgcggca acatgacgac tgacacggtc gttattacgg tgaataatga gcgactgttc
     7261 gtggaagggc cacgtgattc atcgttgttc ctctgtgccg gcgatacgat caagttccaa
     7321 gtctgcgcgc acgactgcga cacgttgacc ttaaccaaag agtgcggtcc gggcaccttc
     7381 ccgccggagc gggatcggtc gccagtctgc ggtaccttga cattcgtgcc gccgtcatcc
     7441 ggcctctaca cctggtgctt caaagccgtt gatcgcttcg gcgcaatggc ggtcgacacg
     7501 gtacggttca acgtgacggt gaattccccg ccgaacatct ccgtgacgcc ctccctgcca
     7561 gagacggtca cagttggcga cacggtctgc tttacggtca ccgggacgga cattgatgcc
     7621 ggggacatcg tcactttgac cgtttccccg ccgggattcc agaagcagtg cagcccgcca
     7681 gggccgaatc gaacctgcac cttctgtgtc acttcgtcgg agccgggaac cttctgtctc
     7741 gtattcaagg ccgcggacaa ctgcggcgca acggccctgg ataccgtctg catcaccttc
     7801 caacagtgct tccgtttcgt catcgacacc gcaactgctt tcctggggca gcacagcgtt
     7861 gtgctttgcg tcaacaatgt ggttgaagcg tttgacccaa gcgtcacccc caacccgtcg
     7921 ggtgtgggcg gcttcaactt cctctttgcc tacgacccga cctgcctcac cttccaaaaa
     7981 gccgaaatgg gtcagttcct taaggactgc cagtgggagt tcttcacctt ccgcttcggg
     8041 acgcgcggga actgcggcac ctgcccctcc gggttcatcc gggtgattgg cattgccgac
     8101 atcaacgatg ccctgcagca ccacccgccg gcgagttgct tccaccggaa cgcgggccag
     8161 tggatttgcc tcttctttga ggtcacccgc gaccggacgt tccagaacac ctgctgcccg
     8221 gtgcgctggg cgtgggagga ctgcacggac aacacggtct ccgacagcac cggtaacttc
     8281 ctctgggtgg tcgattcggt ctacaatgcc catggcgtgc cgattgatct ggcaaccgag
     8341 tacggcatcg gtgtggatgt ctccaattgc gtctcttccg gcgggccggg caaaccgcag
     8401 accatcaagt gcatcgtctt caaggacgga aagatttgca tcccggccga tagcagcatc
     8461 gacgcccggg gcgacataaa cctaaacggc atccccaacg aaattgccga cgccgtactt
     8521 cacgagaact gtctactgta tggtatgagt gcgctctcct ccgacccgct taggcgacag
     8581 gcgcaaattg cggcttccga cgtcaacaac gacggcttgg acatgacagt ggccgactta
     8641 gtttaccagt tgcgaattat cacgggcgat gcccagccct tccccaaacc ggcgggcggc
     8701 gcatctcctg atgtcaccat tgactttgcc gagcggggga gcgagctggt cgtcaccagc
     8761 aataacccgg gccttgatct gggcgggctt ttgctcgtct tgcaggtgga gggcacggtc
     8821 gaaaacgtgg ccgcaaccgg ccgagccggg gccatgggcc tgcgctttgc ccaaacgggc
     8881 gaccagctgc gcatcctcgt cgactcctac gaacggggca aaatcattgc cgcgggaaca
     8941 ggcgagattt taaagatctc gggagtccgc tcggctgagt ttgtggaagg gcaagctgcg
     9001 aaccggggcg ggtaccccgt ggcgctggaa ctggccaagt cgcctttgcc gacgcaattc
     9061 tcgctctctc agaaccggcc caacccgttc aacccgacaa cccggatcac cctggccttg
     9121 cccgttgcca gcgactacac gctggtgatt tacaacgtgg ccggtcaggt ggtgagaacc
     9181 t
//
LOCUS       DBNGZA010000422         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1887045, whole genome shotgun sequence.
ACCESSION   DBNGZA010000422 DBNGZA010000000
VERSION     DBNGZA010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1887045"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="AC16FU_09065"
     CDS             complement(<1..965)
                     /locus_tag="AC16FU_09065"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR2994557.1"
                     /translation="MKLIDLRSDTVTKPSPEMRQAMYDAEVGDDVFEDDPTVQKLEEK
                     VAALFGREASLFVPSGTMGNQVALRTWARPGDELLLEEGAHIFNYEVGGAAALTGLVL
                     HPLPGRNGVLSAEQIEAALRPENIHHPRTRLVSLENTHNRAGGTIYPLTEIQKIRELA
                     QTHGLKMHLDGARIWNAAVAVGVPLSLYGSLFDSVMTCLSKGLGAPIGSVVIGDRDYI
                     AEARRQRKMLGGGMRQVGILAAAGIYAIDNNFPRLAQDHEHAKFFARALAEIPGLKVD
                     LATVETNIVVIDISPSGLAVSEAVLKLKAEGVLVVPFGAQRIRAVA"
     gene            complement(1161..1364)
                     /locus_tag="AC16FU_09070"
     CDS             complement(1161..1364)
                     /locus_tag="AC16FU_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR2994558.1"
                     /translation="MHKGRVKWFNEVRGYGFITEEENGQDLFVHFADIQERGYRTLAE
                     GQEVAFEVTVGDKGPQATNVSKV"
     gene            complement(1496..>1720)
                     /locus_tag="AC16FU_09075"
     CDS             complement(1496..>1720)
                     /locus_tag="AC16FU_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994559.1"
                     /translation="LLQILFAVPTFLFFLRRQWQRNTLSQLVIGSTFTLFVALFFNRY
                     LSDNYLGYMTILLALGFVLREENAGIQISS"
BASE COUNT          360 a          533 c          450 g          377 t
ORIGIN      
        1 gccacagccc ggatgcgctg ggcgccaaag gggacgacca gtaccccttc agccttaagt
       61 ttcaacaccg cctcggacac cgccaagccg ctgggggaaa tgtcgatcac cacgatattg
      121 gtctctacgg tggcgagatc gactttcagg ccgggaatct cggccagtgc ccgagcgaaa
      181 aatttcgcgt gctcatgatc ctgggccagg cggggaaaat tgttgtcgat agcgtagatg
      241 cccgcggccg ccagaatccc aacctgccgc atcccgcccc ccagcatctt ccgctggcgc
      301 cgcgcttcgg cgatgtagtc gcggtcgccg ataaccacgg aaccgatggg cgctcccagg
      361 cctttggaaa gacaggtcat gaccgaatca aagagcgacc cgtacaggga aagcggtacc
      421 ccgacagcca ccgcggcatt ccagatccgg gcgccgtcca agtgcatttt cagtccgtgc
      481 gtctgcgcca actcccggat tttctggatt tcggtcaaag ggtaaatcgt ccctccagcc
      541 cggttgtggg tgttttccag cgagacgagc cgcgtgcgcg ggtggtggat gttttccggc
      601 cgcagtgccg cttcgatctg ctcggcggaa agcacaccgt tccgacccgg gagcgggtgt
      661 aaaaccaaac cggtgagggc ggccgccccc cccacctcgt agttgaagat gtgagcgcct
      721 tcttctaaga ggagttcatc cccgggccgg gcccaggtgc gcagggccac ctgattgccc
      781 atcgtgccgg aggggacaaa aagtgaggct tcgcggccga acagggccgc cactttctct
      841 tccagttttt ggacggtggg atcatcttcg aaaacgtcat cgccaacttc ggcgtcgtac
      901 atcgcctggc gcatttccgg cgacggcttg gtcaccgtgt cgctgcgcaa atctattagt
      961 ttcattgtcc ctgcctcttt cagtcattgc cattctcatt tggattttct catcttaaag
     1021 ctgcttcgct ttatcttact gaggaatgac cctcgtcgtc ttccgctctc tcctttcacc
     1081 cttccggata ttcacgggta acgaaaaatc ccccgccctg gcgggggatc ttccgggctc
     1141 agagccacct cgtcaaggtc ctacactttg ctcacgttgg tcgcttgcgg gcctttgtcg
     1201 cccaccgtga cctcgaacgc tacctcctga ccttcggcca gggtccggta cccccgctcc
     1261 tggatgtccg cgaagtggac gaacagatcc tggccgttct cctcctcggt gatgaatcca
     1321 taccctctca cttcgttgaa ccacttcacc ctgcctttgt gcatcgcacg ctcctccctc
     1381 agagcagttt ctacgctgac aacatctggc acggcttgca tctgtatact tacaaactac
     1441 agcccggaat ccggccttgt caagaaaaaa agatgttcta ttaaccggtt atggttcaac
     1501 tacttatttg aattccggcg ttttcttcac gcaggacgaa cccgagcgcc aaaaggatgg
     1561 tcatataccc caagtagtta tccgaaaggt agcggttaaa aaataaagcg acgaacaaag
     1621 taaaagtcga accgataacc aattggctaa gtgtgttccg ctgccactgc cgcctgagga
     1681 aaaaaagaaa cgtcggcacc gcaaagagaa tctgcaaaag
//
LOCUS       DBNGZA010000423        17925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1144989, whole genome shotgun sequence.
ACCESSION   DBNGZA010000423 DBNGZA010000000
VERSION     DBNGZA010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 17925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17925
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1144989"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..817)
                     /gene="trmD"
                     /locus_tag="AC16FU_09080"
     CDS             complement(71..817)
                     /gene="trmD"
                     /locus_tag="AC16FU_09080"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR2994560.1"
                     /translation="MRIDILTIFPEIFGGYLGSSLIAKAQAKGLVTIRPVDLRQFATG
                     KHREVDDAPYGGGAGMVFMVEPVYRAVQDLVAQVGPHVSRRVVLLSPQGVQFAQTTAQ
                     EFAKTEHLILICGRYKGVDERVKALVVTDELSIGDYVLNGGEAAAMVVVDAVFRLLPG
                     ALGKSDSAETDSVASGLLEYPVYTRPEEFLGQRVPEILLSGHHEEVRKWRRTQALHRT
                     KERRPDLFGRFTLTDEDRELLQEKDRDKEI"
     gene            complement(841..1071)
                     /locus_tag="AC16FU_09085"
     CDS             complement(841..1071)
                     /locus_tag="AC16FU_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR2994561.1"
                     /translation="MKEFVEKIVRSLVEHPDQVQVTEVEAEKTVVLELRVAPDDIGKV
                     IGKHGQNAKALRTLLTAVSARRGKRAVLEILE"
     gene            complement(1278..1523)
                     /gene="rpsP"
                     /locus_tag="AC16FU_09090"
                     /pseudo
     CDS             complement(1278..1523)
                     /gene="rpsP"
                     /locus_tag="AC16FU_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722284.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(1550..2884)
                     /gene="ffh"
                     /locus_tag="AC16FU_09095"
     CDS             complement(1550..2884)
                     /gene="ffh"
                     /locus_tag="AC16FU_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR2994562.1"
                     /translation="MFAQLTEKLESVFKSLRGRGKLTEANIREALVLVRRALLEADVH
                     YRVVKEFLAAVEAKALGREVLESITPGQQVVKIVRDELVHLLGDRAHPLATAPHPPTV
                     ILVVGLQGSGKTTFCGKLAKRLAAQNRPVLLAAADRQRPAAVVQLQSLGRTVGVPVFT
                     GAGTPVEIAQAAVAQAESQLQDYVIIDTAGRLHVDAELLAELAEMKKTAHPHEVLFVA
                     DALTGQAAIEMAQKFQSAVHLDGIVLTKFDGDARGGAALSIRAVVGAPIKFLGVGEKP
                     EDLEVFHPDRLASRILGFGDVVSLVEKVEQAVSAEEARETEEKILAERFTLEDFLSQL
                     QQMKKMGPVESLLGLLPGVNRKALQGAAVDEKALGKVEAMIYSMTRAERSHPEIIDGS
                     RRRRVAAGSGTMPSDVNGLLKQFLAMQKMVRQFKHTAGRRGRGSILSGLGMR"
     gene            complement(2972..3673)
                     /gene="rpe"
                     /locus_tag="AC16FU_09100"
     CDS             complement(2972..3673)
                     /gene="rpe"
                     /locus_tag="AC16FU_09100"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR2994563.1"
                     /translation="MRRQAGHAVKRFYSSQRRQVLVAPSLLACDIRHLADEINKAEAA
                     GADLFHLDIMDGHFVPNLTFGPPVVAAVRQVTRLPLDVHLMITDPHRYLRDYREAGAD
                     FITVHQEAVLHLHRVVAAVKETGACAGVALNPATPFNLLDDILAELDLVLVMSVNPGF
                     SGQKFIPGALRKIHQARERKDTRGHHYLIEIDGGMNGQTAPGAVAAGAEVIVAGNGIF
                     SHRNYRRAIGSLRGR"
     gene            complement(3681..4454)
                     /locus_tag="AC16FU_09105"
     CDS             complement(3681..4454)
                     /locus_tag="AC16FU_09105"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR2994564.1"
                     /translation="MRYSTAGHLPNFLVTHAFWRRMGWILAILAGLWLFFIAFDRVLL
                     PFVVRHGQDREVPNITRAPFDAAEKILTERQLGFAIVGREYHPDEPEGIVISQNPLPG
                     TVVKKGRVVKVFISRGGETALVPALVGLTLRQAQIALLDAGLELGAIGDTTVDTIPVG
                     HVVASQPPAGANLRLGIPVKLFVNQAAQVDSLVVPHLVGRSLAEARLVLGDLGLALGQ
                     IRYEKNDKLLPGTVTEQAPEAGTVVGVPAIVELTVSKTD"
     gene            complement(4522..6111)
                     /locus_tag="AC16FU_09110"
     CDS             complement(4522..6111)
                     /locus_tag="AC16FU_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012550.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR2994565.1"
                     /translation="MKIPKKKDLLRLQAELKSDRKIAEALGGVPQHLIGYWRRKKNIP
                     LRGGPRFTRKEIEFLWEKHGNDRKASSELGISRAAFYRWRKFYGLHEKPKWVRLQKQQ
                     LELHFPRRLILKSGALPQTLVQKIVGRKLAPEAAGTRPQLFLETSVAVAPDRLICHAR
                     QAEALVELPWPDGSAPILERLGFDFSGYDPGLFTGAPLRLWEQAQALKNSVNSVEKEK
                     PNRRLWEAEDRCAPGLLLAGTDRSVAGCGALGSWGIQVDSWDVGGYLTCGRIWTELPA
                     AVRVRLDGRLPESCTVHDLSFRFIHEVYQRDLAGMVIELTGEAVRRLKIPARLLFCAI
                     AAEAGITALFPYDEVTCALGAEKGGAASDPFFPDRQALYAQRIELNMDHPHFWAAGTA
                     GAHQVQELSTLVGTKIAGALVGGCGTGRIEDLKLAAALTEGKELASGAELFVVPDSAP
                     VYLEAIRKGYVKVLVEAGAQILPPGDHWTFATAERMQTLGLLATSPVLLRSELPRPER
                     LWCVSPAAAVRSAVAGEIVLP"
     gene            complement(6108..6623)
                     /locus_tag="AC16FU_09115"
     CDS             complement(6108..6623)
                     /locus_tag="AC16FU_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR2994566.1"
                     /translation="MERIWAPWRKAFILKTREAGCLFCELAAEREDKKSLVVFRGRRA
                     YVVLNRYPYTNGHLMVVPYRHVAELGGMTPGEEKELWALARRSVQLLRQAYQPQGFNL
                     GLNLGRAAGAGVVDHLHRHVVPRWAGDNNFMPVIGNTKIHSLSLDDTFRTLAPGFRQK
                     RLPSQKTRKQR"
     gene            complement(6679..8778)
                     /locus_tag="AC16FU_09120"
     CDS             complement(6679..8778)
                     /locus_tag="AC16FU_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2994567.1"
                     /translation="MQIERCQIKAFGKLKQFTGEFGPGVTVIQGPNESGKSTLVQALC
                     AAFFDSAAGDPGEKEKQGTLVPKNLGEVTLDFKTDGAHYRLHRDFSDGAVKLEKKPGG
                     LTLKDPKGVAEILAGELGLESPELFFATACLRQDESSPTPAASEAVRGKLKEVVTGGP
                     QARAAEAAAQLLKREGEFLQSPDSPDSLPTLEKKQEFLKTEITRLTEELETLRQSRVD
                     FAQIDATLQQVSFSAKEKEAELVTARRLYEAEQALGALAKEFQDLTLRIRELQKSEQV
                     VTTLRATLSGLTPVERQDMTVVEELEARLRYFDSKRAELEAERLQAPEEVPHPSAWLP
                     RVAAYGLGTAGLSALLAVWLWPGFFWIVAGAGSIGGIAAGVFFLQWKIRRDRIMAWEL
                     RKSRLAEVERETLAAQNALRTLLMKYRLPSAAALREQYEKTRDVERQIESEMVRYESL
                     LDGKTQKEIENRLQEVIGELNTLKKVKQELGARTVTAEEVAELEATVATLADEEANLT
                     TAAGALRERLAAAGMGSEELTRLQEERLAVDRSLASMKFRQKLYTLTAEGLLQAQETV
                     QSTVVQVLEHEVAWAVEQLSLGRYSKVRLDPQVFRFDLYSAERKDWVASAEPLSHSAA
                     GQVNLAIRLALLKVLSEREKPVVILDEPFAAFDAEREKQALALLEKLAEEYQIFLFTC
                     RPVPDRWPARGSLVRLA"
     gene            complement(8768..9895)
                     /locus_tag="AC16FU_09125"
     CDS             complement(8768..9895)
                     /locus_tag="AC16FU_09125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2994568.1"
                     /translation="MAKILHTADCKLGSAFPDLGFAGEKVREIQKATFKRIIDLALDE
                     KVDLFLVAGDLFASNLVSKGLVEFVLDEVGRLGEIPAVVLPGDCDAWGPDSVYHWLPF
                     LEVPKNFYLVTLANQPLFFEKIDVSVSGIPVTSSAADESTWKELKKPEEEGHHILLVH
                     GQPVGGKDSDAQPPFVRETAVEKAGFDYVALGHQPAWAEKSLDKGKAVNPGYPEPLDF
                     EQEASGKVAVVKISRSRLDISSETTGSLTWVRQTVPASKLKTAAEIHEMTKKHKGEKN
                     LCEVVLEGEAEHWLDLEKLYDDLAPRFLSLRIDDRRTYPDLRRGSREIVETTILGQYR
                     KFLQGQWDGAEGGDHQLVAAAWTLGQTLLSKDISESAADAD"
     gene            complement(10670..12244)
                     /locus_tag="AC16FU_09130"
     CDS             complement(10670..12244)
                     /locus_tag="AC16FU_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2994569.1"
                     /translation="MTEDKSNRSPEFVLSNQKGREDARWPLTIICAIGILLQGCAATN
                     PPIKNKAVDAKQPKVKIELIPQEKLPRGVPAERATEYALRAILTSKEFAEFEKLKTRE
                     EQEKWVFRYWSERNPRPDRPQQNPYREEVLERILNALERFPAQGTPQPWDDRGEVWIR
                     QGEPKDRLIQESGSDSMWTKEVWTYPSIEYTFKDLNGTGEFRLTEAGYFGITPRSPSD
                     IAMTTSKMVATVRQQGVHYAYDYGKALKVIDRVEKFRQAEKSTGSDVFIQWAVRLRDL
                     LKPFSDTAKYSWRVVAYDLNGNVVCQDSASETRKITTPVDGQVFINQCRLWLPPGNYT
                     YALAVEGSGGLGLIKRKEPLIIPEYVTLRERSISDLMLPSLMKDVTGDSIPAELLRFV
                     RTFEGHEYLIVPSTGQIFQGDLLNLYAELYYLTLDTTTEKGVSRFRAKISLVSQKGQT
                     KRFLLADQELEGEGQHVPLLKAFSTEKIPPGEYIVNVEVEDLLAKPTDSKRKLTILGP
                     VKINKRSDIQSENSEK"
     gene            complement(12325..14982)
                     /locus_tag="AC16FU_09135"
     CDS             complement(12325..14982)
                     /locus_tag="AC16FU_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR2994570.1"
                     /translation="MRTRGPKAGISIVGGLVFFLWVAAALQAQSPADTTTAEIKITTW
                     FLAGPVEGFLPVFGDTLKAIREKLPTTEYLDFDKLWPQAGKEIAWSPGQTITWQSFNS
                     PDGKPQFPIRSDAKSPHVAYAACYLASPRWQKVKLVVTTGNLFALYLDGEEKAKQTAV
                     SKAAKDTVETSAELVLTQGKHRLVIQTVFDPRDSLAFWSLSATFRVPKEKKVSAPQVS
                     LDPKHAPSLDDLVELESISELAISQDATKLAVVKRFWKVKDDKSATYLEIRALPSGQL
                     LQTQRMGDGVSTPVWSPDGERLAFQAPGDKEGNDLWLLEITSGRVDRLIAGEKGLGNV
                     IWAPDGKFIYFSSTEPKPEQKDQPAYERLNELYERWNYWKDKSHLNLISVVDKTRHHL
                     TTGEYAIDDAALSPDGKKLALVRSVPIPERPFFATELWLFDPGARTEEKLANLRRQVG
                     HLVWSPDGSALALSASVGEVTGDTSAVEHNNYNYDLFLLSLSDRNLKNLTLNFDPHVG
                     AALTGASDGRSPLWWSAQDKKIHFLATNRGRVNLYRVDPAKPGIVEPLNLPFPVSSGT
                     DVARNGRDLVFIGSSPTQMPQVYHYDLQRKKSTQVLTLNGELTTRLVLGKVEPWDFTN
                     ARGTKIDGWIYYPTDFAPDKKYPLVVYYYGGVFPNGEAFGFTYHWWNAHGYLVYVLNP
                     VGAVGYGQKFADLHVNDWGELAAADIIEGVQKLSREKPCIDSTRVGAYGGSYGGFMTM
                     TLATKTKLFGALCSMYGIADITSYWGEGWWGYLYSDVATAKSYPWNRKDVYIARSPLY
                     NADKITTPLLLLHGTGDTNVPPGESEQMFTALKILKQDVAYVRFPGEGHGISNKLSNY
                     LSHRQMMLEWFDKYLKDQPEGWEERWKKK"
     gene            15377..15787
                     /locus_tag="AC16FU_09140"
     CDS             15377..15787
                     /locus_tag="AC16FU_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994571.1"
                     /translation="MKHTLISVKLITLTTFVFLFAGCGGSKNNALAPFQPQINNAPDN
                     FQFQATGIQKVTTTLQYTWQNSGTSATVNQACAITAGTATLVILDASSTQVYSRDLSA
                     NGTFATTAGTAGAWTIRVILSNLSGTLNFRVQKV"
     gene            15980..17674
                     /locus_tag="AC16FU_09145"
     CDS             15980..17674
                     /locus_tag="AC16FU_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994572.1"
                     /translation="MPALAADKEKLLYAFPPPPTPDAVEWPGSPLGAFNLITRTKGRT
                     QVHDKTTDAKPGKRDALVASAQRAIYRSFPNTILEHDVVIHGVRVRAITNSPHLYDFW
                     VDNWYSVREWETLTGQVPPHEPKIMVYALGGVSEEPEAAYYSRQTNTIIFFNTSYYGQ
                     LKSWVLGAVGRILAEEEGIHSIHGACVERNGKGILYIAPTGTGKSTSSYGLMNAGNCR
                     FHSDDWVYVRYAGHLRDGSLFVPMRILEEKRKVAEGFAVYRWLEANGKTKSDATLEGV
                     DLQNKVKRIRVGDLDFSRPLEAYAYTSEKVFYLRSNLVESFPEVAYHILHSKIENGPD
                     ATLDFVADQKSKIEGLYAQLERAQNPTVRRYFAQIDPARVKETLARLYAFDNTRAMLD
                     IARVFGPGNIFANPLEPVHLTTVMLLKRDFGDPLVLDWLSLDEFMTWLLIGETPDKKR
                     EIAFNAYRAVDDETEKKFLVKVQEANGGGIDPNFYGTYVSQPNIPPTLLQEFELFRVM
                     YQATNCYVMNTVLQKDPKIKGKQEAVQRSMALIARTIDRQPKDVHLTLENYVDFLA"
     gene            17703..>17925
                     /locus_tag="AC16FU_09150"
     CDS             17703..>17925
                     /locus_tag="AC16FU_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994573.1"
                     /translation="MGRTLSFGLTVLALFIVSATAQEKKEMAKPVEPTGLKVGDPAPD
                     FTLPYATKDTLVFQGGIKLSDFKGKKNVLV"
BASE COUNT         3585 a         5516 c         4936 g         3888 t
ORIGIN      
        1 tcggccgctt tgagtaaatc catgtcacta tcctctcttt tttctctcgc ggggcgatgc
       61 tcccggccct ttatatttcc ttatcccgat ctttttcctg caggagttct cgatcttcgt
      121 cggttaaggt gaaacggccg aacagatccg gacgccgttc tttggtccgg tgcaacgcct
      181 gggtccgccg ccactttcgg acttcctcgt ggtggccgga aagcaaaatc tccggcacgc
      241 gctgccccag gaactcctcc ggccgggtgt agaccgggta ttccaggagg ccgctggcaa
      301 ccgaatcagt ctcggccgaa tcactcttcc ccagagcgcc cggcaagagc cggaagacgg
      361 catccaccac gaccatggct gccgcctcgc cgccgttgag gacgtagtcg ccgatcgaaa
      421 gttcgtcggt caccaccagc gctttcaccc gttcgtccac acctttgtac cgtccgcaaa
      481 tgagaatcag atgctccgtt ttggcaaact cttgagccgt ggtctgggcg aactgcacgc
      541 cctgcggcga caagagcacc acccggcgcg aaacgtgcgg ccccacctgg gcgacgaggt
      601 cctgaacggc gcggtagacc ggctcaacca taaagaccat ccccgctccg ccgccgtacg
      661 gcgcgtcatc gacttcgcga tgtttgccgg tggcgaactg ccgcagatcc accggccgga
      721 tcgtcaccaa gccctttgcc tgggccttgg caatcaggct cgacccgagg tacccgccaa
      781 aaatttccgg aaatatggtc aggatgtcaa tgcgcatcgt gctcggtttc gccgccgaaa
      841 ctattcgagt atttccagta ccgctcgctt gccccgtcgg gccgagacgg cggtcaaaag
      901 ggtgcgcagc gctttggcat tctggccgtg cttgccgatg accttgccga tgtcatccgg
      961 agcaacgcgc aattccagca ctaccgtctt ttccgcttcc acctcggtca cctgcacctg
     1021 gtccggatgc tccaccagcg agcggacgat cttttcaacg aactctttca ttccgcacct
     1081 cccggatacc tcaccgaggt gggacagccc tcctccgctg ctcagccgtc gctgtaggaa
     1141 agctgcgcgc gctacgcagc gggggcggtg ggggcttgct cctgcttgac cttgcgcttc
     1201 ttggccttct tgcgttcggg taactcagtc aagagcgtca tcgtggcgac atcggctcct
     1261 ttcgccagcg cctgccactt ccggttcacg ccaatccggc ggaagagggc gttcaccgct
     1321 tccgtggggc gggcgccctg cctgagccac tggaaaacct tttcttcatc cacgaccacc
     1381 ctggccggtt tcgcaatcgg gtgatagtac ccgaccgtct cgaggaaacg gccattccgg
     1441 gggtctgtgc tctccgccgc caccagccgg aagaacggcc gcttcttggc acccatacgt
     1501 cgcaaccgca gtttaaccgc caaacccacc tcctgttttc tctttttcgc taccgcatac
     1561 cgagacccga gagaatggac cccctccccc ggcgtccggc ggtatgcttg aactgacgga
     1621 ccattttctg catggccaaa aactgcttca agagcccgtt cacgtcggag ggcatcgtgc
     1681 ccgatccggc ggctacccgg cgccgccgcg acccatcgat gatttcgggg tggctgcgtt
     1741 ccgcgcgggt catggaataa atcatcgcct ccactttccc gagcgctttt tcatcgaccg
     1801 ctgcgccctg caaggccttg cggttcacgc cgggtaagag gcccaaaagc gactccaccg
     1861 ggcccatctt tttcatctgc tggagctggc tcaaaaaatc ttcaagggtg aaccgctccg
     1921 ccaggatttt ctcttccgtt tcgcgggcct cttcggccga gaccgcctgc tctactttct
     1981 ccaccagcga aacgacgtcg ccgaaaccga gaatgcggga cgccagccgg tcgggatgga
     2041 aaacctccag atcctcgggc ttttccccga cgcccaagaa cttaatcggc gcgccgacga
     2101 cggcacggat cgaaagcgcc gccccgccgc gggcatcacc gtcgaatttg gtgagcacga
     2161 tgccgtccag atgcacggcg gactgaaatt tttgcgccat ctcgattgct gcctgaccgg
     2221 tcagagcgtc cgccacgaag agcacttcgt gggggtgtgc cgtttttttc atttccgcca
     2281 gttccgccag aagttcggca tcgacatgca gccgtccggc ggtgtcgatg atgacgtagt
     2341 cctggagctg gctttcggcc tgcgccacgg ctgcctgcgc aatttccaca ggcgttccgg
     2401 cgccggtaaa aaccgggacc cccaccgtcc ggccgaggga ttgtaactgt acgaccgcag
     2461 ccggtcgctg ccgatcggcc gctgctaaga gaaccggccg gttttgcgcc gcaagacgtt
     2521 tcgccagttt cccgcagaag gtggttttgc cggagccctg caagccgacc actaagatca
     2581 ccgtcggcgg gtgcggcgcg gtcgccaggg ggtgagcccg gtcgcctaag aggtggacca
     2641 attcgtcgcg gacgattttc accacctgct gcccgggggt gatgctttcc agcacttccc
     2701 ggcccagcgc cttggcttcc accgcagcca gaaactcttt gaccacccgg tagtggacgt
     2761 cggcctccaa aagcgcccgg cggaccagca ccaaagcctc gcggatattc gcctcggtca
     2821 gtttgccgcg gccgcgcagg gatttgaaaa ccgactcaag tttttcggtc aactgtgcaa
     2881 acatattttt ccgctcacca gccggaggac gcattcttaa ccggagggag aactttttat
     2941 tataacaaat ttttgagaat gtgcaactgg actaccggcc gcggagtgag ccgatcgccc
     3001 gacggtaatt acggtggctg aaaattccgt tccccgcaac gatgacctca gccccggcgg
     3061 caaccgctcc cggagcggtc tggccgttca tcccgccgtc gatctcgatg aggtagtgat
     3121 gtccccgagt gtccttacgc tcccgggctt gatgaatctt acgcagggca ccgggaataa
     3181 atttctggcc gctgaaaccg gggttcaccg acatcaccaa taccaaatcg agttcagcca
     3241 ggatgtcgtc cagcagattg aagggggtcg ccggattgag cgccacaccg gcgcaggcac
     3301 cggtttcctt gacggcggcc accacccggt ggaggtgcag caccgcttcc tgatgtaccg
     3361 tgatgaaatc ggcccccgct tcccggtaat cccgcaggta tcggtgggga tcggtgatca
     3421 tcaggtggac gtccaaaggg aggcgggtca cctggcgtac cgccgccacc acgggggggc
     3481 cgaaggtcag gttggggacg aagtggccgt ccatgatatc gaggtggaag aggtccgccc
     3541 cggccgcctc cgctttgttg atctcgtcgg ccaagtggcg gatgtcgcag gccaaaagcg
     3601 aaggcgccac cagaacctgt cgccgctgac tggagtaaaa ccgcttgacc gcgtgcccgg
     3661 cttggcgtct cacggatagc tcaatccgtt ttgctcactg tcaattccac gatggcaggt
     3721 acccccacga cggtgcccgc ttcgggcgcc tgctcggtga ccgtcccggg taacagttta
     3781 tcatttttct cgtagcggat ctgtcccagc gcgagcccca gatcacccag gaccaaacga
     3841 gcttccgcca aactccgccc caccaggtgc ggcaccacca gcgagtcgac ctgggccgcc
     3901 tggttgacga acaatttcac cggaattccc aggcgcaggt tggccccggc cggaggctgc
     3961 gaagccacca cgtggcccac tggaatggtg tccaccgtgg tgtccccgat ggcacccaac
     4021 tctagtcccg cgtctaaaag cgcaatttgc gcctggcgca gggtgagccc caccagggcc
     4081 ggaacgaggg cggtttcgcc gcctcgggaa atgaaaacct tgaccacacg cccttttttc
     4141 accaccgttc cgggcagggg gttttgcgaa atgacgatcc cctccggctc atcgggatgg
     4201 tattcccggc ccacaatggc aaaccccagt tgccgttcgg tgagaatttt ttccgccgcg
     4261 tcaaagggag cccgggtgat attcggcact tcccgatcct gcccgtgccg aacgacaaag
     4321 ggcaaaagaa ctcggtcaaa ggcgataaag aaaagccaca gacccgccag aatggcaaga
     4381 atccagccca tccgccgcca gaaggcatgc gtgaccagaa agttcggaag atgtccagcc
     4441 gttgaatacc gcactcgccc tttcgcccga gaatatggcc gacattgccc gtttgagcaa
     4501 tgccccggac cacccgaaag gttacggcaa cacgatctcg ccggctacgg ccgaccggac
     4561 cgccgccgcc gggctcacgc accagagccg ttccggacgt gggagctcgg agcggagcag
     4621 caccggactg gtggcgagga gacccaacgt ttgcatccgc tcggcagtgg caaaagtcca
     4681 gtgatccccg gggggcagta tttgagcccc tgcctcgacc agcactttga cgtacccttt
     4741 gcggatcgcc tctaagtaaa ccggagccga atcgggcacc acgaaaagct ctgccccgga
     4801 ggccagttct ttgccctccg taagagcggc cgccagtttc agatcttcta tccggccggt
     4861 gccgcaaccg ccgacgagcg ctcccgctat tttcgtcccc accagtgtgg acaactcctg
     4921 tacctggtgc gcaccggctg ttcccgcggc ccagaagtgg gggtggtcca tattgagctc
     4981 aattctctgg gcgtacaacg cctgcctgtc cgggaaaaac gggtcactgg ccgccccccc
     5041 tttttcggct ccgagagcgc aggtaacctc gtcatacgga aaaagagcgg taatcccggc
     5101 ctcagcggct atggcacaga acaaaagccg agcgggaatt ttcagccgcc gcaccgcctc
     5161 tccggtgagc tcaatcacca tccccgctag gtcgcgctgg tacacttcgt ggatgaagcg
     5221 gaaactcagg tcgtgcaccg tgcagctctc cggcaagcga ccgtccagcc gcacccgtac
     5281 tgcagcgggc agttcagtcc atatccggcc gcaggtgagg tatcccccga cgtcccaaga
     5341 atcaacctga attccccaac tccccaaagc cccgcagccg gcgacactcc ggtcagttcc
     5401 agccaagagg agacccggag cgcaccggtc ttccgcttcc cagaggcggc ggtttggctt
     5461 ttctttttcc actgaattga cgctgttttt gagtgcttga gcttgctccc acaagcgcaa
     5521 gggcgcaccg gtgaagagcc ccgggtcgta tcccgagaaa tcgaaaccaa ggcgctcgag
     5581 gatgggagcc gacccgtccg gccaggggag ttccaccagc gcctcggctt gccgtgcatg
     5641 gcagatcagc cggtccgggg ccacggcgac actggtttcc agaaacaatt gcggccgggt
     5701 ccccgctgct tccggcgcca gctttcgccc gacaattttt tgcaccagtg tttggggaag
     5761 cgctccggat ttcaggatca accgccgcgg gaagtgcagt tcgagttgct gtttttgcag
     5821 ccggacccac ttcggttttt catgcaggcc gtaaaatttg cgccagcgat agaatgcggc
     5881 ccgcgaaata cccagttcgc tactggcttt ccggtcgttc ccgtgctttt cccagaggaa
     5941 ctcaatttcc ttccgggtaa agcggggtcc gccgcgcagg ggaatgttct ttttccgccg
     6001 ccagtagccg atcaaatgct gcggcacgcc gcccagggcc tcagcgatct tgcggtcgct
     6061 tttcagttcg gcctgcagcc ggaggaggtc ttttttcttg gggatcttca tcgctgctta
     6121 cgggtcttct gcgacggtaa acgtttttgc cgaaaaccag gcgccagggt gcggaaggta
     6181 tcgtccaggg aaagggagtg gattttggtg ttcccgatga ccggcatgaa attgttgtcc
     6241 ccggcccacc gcggcaccac gtgccggtgc aggtggtcga ccacgccggc gccggcagcc
     6301 cggcccagat tcagcccgag gttaaatccc tggggttggt aggcctgccg caaaagctgc
     6361 acactccgcc gggccagtgc ccagagctct ttttcttcgc cgggggtcat ccctcccagc
     6421 tcggccacat ggcgatacgg caccaccatc aggtgaccgt tggtgtaggg gtagcggttc
     6481 agaacgacgt aggcccgccg gccccggaac acgacgagac tctttttgtc ctcccgctcg
     6541 gccgccagtt cgcagaagag gcaaccggct tctctggttt tgagaatgaa tgccttgcgc
     6601 cagggtgccc agatccgttc cattggcccg gcaatatatc ccgctgcggt cgacccgcca
     6661 atcccgcccc cgcaggattc aggccagtct caccagactg cctcgggcgg gccaccggtc
     6721 cggcaccggt cggcaggtaa aaaggaaaat ctgatactct tcggcaagtt tctccagcag
     6781 ggccagggcc tgcttttctc gttccgcatc aaaggcggcg aacggttcgt ccaaaatcac
     6841 caccggcttc tcgcgctcgg agagtacttt gagcaaggcc agccggatgg ctaagttgac
     6901 ctgtcccgcc gcgctgtgcg aaagcggctc agcggatgcg acccagtctt tgcgctcggc
     6961 cgagtacagg tcaaaccgga agacctgcgg atcgaggcgc acctttgaat accggcccag
     7021 agacaactgc tccaccgccc aggccacttc atgttccaga acctgcacca cggtgctctg
     7081 caccgtctct tgggcctgga gcaggccttc ggccgtgagg gtatagagtt tttgccggaa
     7141 tttcattgac gcgagggacc ggtcgaccgc cagacgctct tcctgcaacc gggtgagttc
     7201 ctcactgccc atcccggcgg cagccaatcg ttcccgtaag gcgccggccg ccgtcgtgag
     7261 attggcctct tcgtcagcca gggtggcgac ggtggcctcc agctccgcca cttcttccgc
     7321 cgtaacggtc cgggccccca gttcctgctt cacctttttc agcgtgttca gttctccaat
     7381 aacctcctgg agccggtttt caatctcctt ctgcgtcttc ccgtccagta aactttcgta
     7441 gcgcaccatt tcgctttcaa tctggcgctc cacgtcccgc gttttttcgt actgttcccg
     7501 cagggcggcc gccgacggca accggtactt catcaagagt gtgcgcagcg cattctgggc
     7561 cgccagcgtt tcccgttcca cttcggccag ccggctcttg cggagttccc aggccataat
     7621 ccggtcgcgg cggattttcc actgcaagaa gaaaactccc gcagcgatgc cgccgatcga
     7681 ccccgccccg gcgacgatcc agaagaaacc cggccagagc cagacggcca gaagcgcgga
     7741 cagccccgcg gttcccagcc cgtaggccgc cacccgcgga agccaggcgg aagggtgcgg
     7801 cacttcctcc ggtgcctgga gccgttcggc ttccagttcg gcccgtttgg aatcgaaata
     7861 ccggagccgc gcttccagtt cttccaccac ggtcatatcc tgccgctcga ccggcgtcaa
     7921 tcccgacagt gtggcccgca gggtggtaac gacctgttcg gatttctgca gctcccggat
     7981 gcgcagggtg aggtcctgga actcctttgc cagcgctccc agcgcctgtt cggcctcata
     8041 gagacggcgg gcggtgacca gttccgcctc tttttctttt gcggaaaacg acacctgttg
     8101 cagggtggcg tcaatttggg caaagtcgac gcggctctgg cgcagcgtct ccaattcttc
     8161 ggtcaggcgg gtgatctctg tcttcaggaa ttcctgcttt ttttccaggg tgggtaagct
     8221 gtccgggctg tccgggctct ggagaaactc cccttcccgc ttgagcaact gggccgccgc
     8281 ttcggccgcg cgggcctgcg gtccgccggt gaccacttcc ttgagtttgc cgcgcaccgc
     8341 ttcgctcgcg gcgggagtgg gggaagattc gtcctgacgc agacaggccg tggcaaaaaa
     8401 gagctccggg ctttccagac ccagctcgcc ggctaaaatc tcggcgaccc ccttggggtc
     8461 cttgagcgtc aggccacccg gtttcttttc caatttcact gcgccgtcgg aaaaatcccg
     8521 gtgcagacgg tagtgcgccc cgtcggtttt gaaatccaag gttacctctc ccaaattttt
     8581 cgggaccaga gtgccttgct tttctttctc accggggtcc ccggccgcgg aatcaaaaaa
     8641 agccgcgcag agcgcctgca ccaaagtcga tttgcccgac tcgttgggac cctggatgac
     8701 cgtcaccccc ggcccgaact cgccggtaaa ctgcttcagt ttaccgaacg ctttgatctg
     8761 gcaacgctca atctgcatcg gccgccgatt cgcttatgtc ttttgagagc agcgtctgcc
     8821 ccaacgtcca ggcggcggcg accagctggt ggtccccgcc ttccgctcca tcccactgcc
     8881 cttgaagaaa tttccggtac tgtcccagaa tggtcgtttc gacgatctcc cgggatccgc
     8941 ggcggaggtc gggataggtg cgccggtcgt cgatccgcag ggagaggaag cgcggcgcca
     9001 aatcgtcgta caatttctcc aggtcgagcc agtgttccgc ctcgccttcc aagaccacct
     9061 cgcagagatt tttctccccc ttatgtttct tggtcatctc gtgaatttct gccgcggttt
     9121 tcaacttact ggcgggcacg gtctgccgga cccaggtgag ggaaccggtc gtttcggagg
     9181 aaatatccag ccggcttcgg ctgattttga cgaccgccac tttgcccgaa gcctcttgct
     9241 caaaatcgag cggttccggg tatcccggat tcaccgcctt ccctttgtcc agacttttct
     9301 ccgcccaggc cggctggtgc cccaaagcca cgtagtcgaa tccggccttc tccactgccg
     9361 tctcgcggac gaaaggcggc tgggcgtcgg aatctttacc cccgacgggc tgaccatgca
     9421 cgagcaaaat gtggtggcct tcctcctcgg gtttcttgag ttctttccag gtcgattcgt
     9481 cggcggcgct gctcgtgacc ggaatcccgg aaactgaaac gtcgattttt tcaaaaaaaa
     9541 gcggctggtt cgccagagtc acaaggtaga agtttttcgg cacctccaga aacggcagcc
     9601 agtggtaaac cgaatccggc ccccaggcgt cacaatcgcc gggcaaaacg actgcgggaa
     9661 tctctcccaa ccgtcctact tcatcgagga caaactcgac cagacctttg gaaactaaat
     9721 tggaggcgaa cagatcgccc gccaccaaga agaggtcgac tttttcatcc agagctaagt
     9781 cgatgattct tttgaaagtg gccttctgga tttcccgcac tttctccccg gcaaagccca
     9841 agtcggggaa agcgctacct aatttgcaat cggcggtgtg cagaattttg gccatagtca
     9901 tccggaaaca aaaggcacaa aaccgtcttc tgtgccccga attgacgcta cttatgtatc
     9961 ggcaaaccgg aaggggatat ttaggttacg aggtaaaccc cagcaggtca ggcggtgggg
    10021 tagtcgcgtg gtaaagccag ctcgactatt ccttgtgtaa acaaggagta ataaaaagcg
    10081 gagggatcac ttcggtgatc cctccgctta gagcccacca gtgttattgc tggtaaggag
    10141 ggtacgtact accgcaggta caggcgtttc ctcctgagcc cgcttcacac tccacccacg
    10201 ttaattcaca acattcctga ggccaacagt ctatggtgcc gcagattatc ccgtgatgtt
    10261 gcacaatcac gcactgcgga gtaccaccgc cgcccactgc cgtcagggtg gaagagccga
    10321 aattctttgg gtacgtgctg tagacaacct gcccgtttac ccacacctta gtgtatgatg
    10381 agccgagcac gtgcgcaacc tctacgtttc tcccggccag tacccgccag gtaaagggca
    10441 tacgtcgtta aatacccacg ttttgacgaa ggccttccca cccatcttcg cctgactaac
    10501 ttgtttggct gttaggaagg agacgaagaa gaccagaaca aatatggtcg agacggtgag
    10561 gattttttgt ttctgcatcg tcattcacct ccctgtgcag attttaggtt tttcgttaaa
    10621 tccgctgcgt tcctttgctg cgtggggtgc ttcgctcgac cacctccttt cacttttccg
    10681 aattttcaga ttggatgtca gaccttttgt tgatcttcac gggccccaag attgttaatt
    10741 tccgttttga atccgtaggc ttcgccaaga gatcttccac ctcaacatta acgatatact
    10801 caccgggcgg gatcttttct gtggagaatg ctttcaacaa gggcacatgt tggccttcac
    10861 cttcaagttc ctggtcggca aggagaaacc gtttggtttg acccttctga gagactaagc
    10921 taatcttcgc ccgaaaacgg cttactcctt tctctgttgt tgtatcaagg gtgagatagt
    10981 agagctcggc gtagagattt aggagatctc cttggaagat ttggcctgtt gagggaacaa
    11041 ttaggtactc gtggccttcg aaagttcgaa caaagcgaag tagttcggct ggtattgaat
    11101 cgcccgtcac gtctttcatg aggcttggga gcataagatc acttatagaa cgttcccgga
    11161 gggtgacgta ctcaggaatg atgagtggtt cttttcgctt tataagaccg agcccaccag
    11221 aaccctccac agccagagca tacgtatagt tccctggtgg aagccagagc cggcattggt
    11281 tgatgaatac ctgtccatca accggtgtgg tgattttacg ggtttcactc gcgctatctt
    11341 ggcaaacgac attcccgttg aggtcgtaag cgaccacacg ccagctatac ttcgccgtgt
    11401 cgctgaacgg cttcaatagg tcacgcagac gaaccgccca ctggataaaa acatccgaac
    11461 ccgtcgattt ttccgcttgc cggaactttt ccacgcggtc tatgacctta agtgctttcc
    11521 catagtcgta agcgtagtga acgccctgct ggcggacagt tgcgaccatt ttggatgtcg
    11581 tcatcgctat gtcgcttgga ctgcggggag tgattccaaa gtacccagct tcggtgagcc
    11641 tgaactcccc tgttccattc agatccttaa acgtgtactc tatcgagggg tatgtccaga
    11701 cctccttcgt ccacatactg tcactcccgc tctcttggat cagccgatct tttggctccc
    11761 cttgacgtat ccagacttca ccgcggtcgt cccacggctg gggcgttccc tgcgccggga
    11821 atcgctccaa ggcgttgaga attcgctcca gcacttcttc tcgatagggg ttttgctggg
    11881 gccgatcggg ccgggggttt cgctcgctcc agtaacggaa gacccacttc tcctgttcct
    11941 ctcgtgtttt gagtttctcg aactctgcga attccttcga ggtaagaatg gctcgtagtg
    12001 cgtactcggt cgcccgttct gctggtacac cccgtggtaa tttttcctgc gggattaatt
    12061 caatcttgac ttttggttgt ttcgcatcaa ccgccttgtt cttgatcggc ggatttgtag
    12121 ctgcgcatcc ttggagcagt atccctattg cgcaaataat ggtgaggggc caacgggcat
    12181 cctcccttcc tttttgattg ctaagaacga attctgggct tcggttgctt ttgtcttcgg
    12241 tcataccgtg ttttcctacc cccgtctcca tgacggcaat ctacgatacc ccccagattt
    12301 gccaagtagt tttggaaacc tgatttactt cttcttccac ctttcctccc acccctccgg
    12361 ctggtctttt aagtacttgt cgaaccattc caacatcatc tgtctgtggg agaggtagtt
    12421 gctcaatttg ttgctgatcc cgtgcccctc tcccgggaag cggacgtagg ccacgtcctg
    12481 cttgaggatt tttagcgccg tgaacatctg ctccgattcg cccggcggga cgttcgtgtc
    12541 gccggtgccg tggaggagca aaagcggggt ggtgattttg tcggcattgt agagaggcga
    12601 gcgggcgatg tagacgtctt tccggttcca ggggtaactt ttggcagtgg ccacgtcact
    12661 gtagagataa ccccaccaac cctcgcccca gtaactggtg atgtctgcga tgccgtacat
    12721 ggaacagagg gcaccgaaga gtttggtctt ggtcgccaga gtcatcgtca taaagccgcc
    12781 gtaactgccg ccgtaagcgc cgacgcgcgt cgagtcgatg cagggcttct cgcgggataa
    12841 cttctgcacg ccttcgatga tgtcggcggc ggccaactcc ccccagtcgt tcacgtgcag
    12901 atcggcgaac ttctgcccgt accccaccgc gccgaccgga ttgaggacgt agacgagata
    12961 gccgtgcgca ttccaccaat ggtaggtgaa tccgaaggct tcgccgttgg gaaagactcc
    13021 cccgtagtag tagacgacca gcgggtactt cttatccggc gcaaaatcag ttggatagta
    13081 aatccagccg tcgattttcg tcccgcgcgc attggtgaaa tcccagggct caactttgcc
    13141 cagcaccaac cgagtggtca gttcgccatt caacgttaag acctgagttg atttcttccg
    13201 ctggagatcg tagtggtaga cctgcggcat ttgcgtgggc gaagaaccga taaagacgag
    13261 atccctaccg ttgcgggcca catccgtacc tgaggaaacc gggaaaggca gattgagcgg
    13321 ctcgactatg cccggtttgg ccggatcgac gcggtagaga ttcaccctcc cccgattggt
    13381 agccaaaaag tggattttct tatcctgcgc actccaccag aggggcgagc ggccgtccga
    13441 ggcgcccgtg agcgccgcac caacgtgcgg gtcaaaattc agtgtgagat ttttgagatt
    13501 tcgatcggaa agtgaaagca agaataagtc gtaattgtag ttattgtgtt cgaccgcact
    13561 cgtgtcgccg gtcacttccc cgacgctggc gcttagcgcc aacgcactgc catccggcga
    13621 ccagaccagg tgccccacct gccggcgcag attcgctagt ttttcctcag tgcgtgctcc
    13681 cggatcaaag agccagagtt cggtggcaaa aaaggggcgc tcaggaatcg gcacgctgcg
    13741 caccagcgcc agttttttcc cgtccggtga taaagccgcg tcgtcgatgg cgtactcccc
    13801 ggtggtcagg tgatggcgcg ttttgtccac caccgagatt aaattcaggt gtgacttatc
    13861 cttccagtag ttccagcgct cgtaaagttc atttaacctt tcgtacgccg gctgatcttt
    13921 ttgctccggc ttcggttcgg tggaggaaaa atagatgaac ttgccgtccg gcgcccagat
    13981 gacgttcccc agccctttct ctccggcgat caaccggtca acccggcctg aggtgatttc
    14041 gagcagccag aggtcgttcc cctctttatc ccctggcgcc tggaaagcca ggcgctctcc
    14101 gtcgggcgac cagaccggcg tggaaacgcc gtctcccatt ctttgcgtct gcaggagttg
    14161 acccgaaggc agcgcgcgaa tttccaagta ggtcgccgac ttgtcgtctt tgaccttcca
    14221 gaaccgcttg accacggcca gcttcgtcgc atcctgggaa atggctaatt cggaaatcga
    14281 ctccagctcg accagatcgt ccagggaggg agcgtgtttg ggatcaagcg agacctgcgg
    14341 tgcagatact ttcttttcct tcggaacgcg gaaggtggcg gagagtgacc agaaggccaa
    14401 agaatcgcgc ggatcaaaga cggtttgaat caccagccgg tgtttccctt gcgtgaggac
    14461 aagttcggcg gatgtttcga cggtatcctt tgcggctttc gacaccgcgg tctgcttagc
    14521 tttctcctcc ccgtctaaat agagcgcaaa caggttgccg gtggtgacga ctaacttcac
    14581 cttctgccag cgcgggctgg cgaggtaaca cgcggcgtag gccacgtggg gtgatttcgc
    14641 gtcagagcgg attggaaact gaggttttcc gtcgggagaa ttgaaagatt gccacgtgat
    14701 ggtctgaccc ggactccagg caatttcttt tcctgcctga ggccagagtt tatcaaagtc
    14761 gagatactct gtggttggca atttctcccg gattgccttc agcgtatcgc cgaacaccgg
    14821 cagaaaaccc tccaccggcc cggccagaaa ccaggtggta atcttgatct cagcagtggt
    14881 ggtatccgcc ggtgattgcg cctgaagcgc cgcggctacc cagagaaaaa agaccaatcc
    14941 acctacgata gaaataccag cttttggacc gcgtgtgcgc acgctcgctc tcctggtaat
    15001 gagttgacga tgttcagcca ttttgcgtct tcgaagggct cgcaaggtga ccataaatag
    15061 gtttctcagt ctcgaatata tgcagaggcg ggcgggcgac aaccactcgg gttttctccg
    15121 tacgaagctc gaaccaaata aggtacaaat tgatggaaaa tgtccgcatt ccttcagact
    15181 ttttcctttc ggtcgacagg ataagtaatc ccttgctcaa taaatggtgg gaaggaacgt
    15241 atcctataga gcagcaagag aaaaggagcc cgaaaatgcc caatgtcgta ttgagccgtc
    15301 gtaaacgact acccaacggg ttctctctat caccacgggg ctcgacgcaa tccggataca
    15361 cgaaaggagc tggagaatga aacacaccct catctcagtc aaactgatta ccctgacaac
    15421 cttcgtcttt ctctttgccg gctgcggtgg cagtaagaat aacgccctgg ctccatttca
    15481 gccccagatc aacaacgctc cggataactt ccagtttcag gccaccggca tacagaaggt
    15541 gactaccacc ctccagtaca cctggcagaa cagcggcacc tccgccaccg tgaaccaggc
    15601 gtgtgccatc acggcgggaa ccgccaccct ggtgattctg gacgcctcca gcacccaagt
    15661 ctactcccgt gatctcagcg ccaacggcac cttcgccacc acggccggca ccgcgggcgc
    15721 ctggacgatt cgcgtcattc tgtcgaatct cagcggaacg ctgaacttcc gggtacagaa
    15781 ggtgtgaaca gtcggctgat ctttctcact tcgcttgtac gaagaggtta aagggcgact
    15841 ttaacctctt ttcattttat atacttccgc ccagttcaat ttttttgccg tcttgccaaa
    15901 gcttctcttg gcttttatac tgggtttcat gtctgtactg cggaaacaaa gacgctaacc
    15961 gagttgagaa aatcaggttg tgcccgcact ggccgcagat aaagaaaaac tcctctacgc
    16021 ttttccgcca ccccccacac ctgacgcagt cgaatggccg ggatcccctt tgggtgcttt
    16081 caacttaatc acccgcacca aagggcgcac gcaggttcac gacaaaacca cagacgccaa
    16141 accggggaaa cgggacgcgc tggtggcatc ggcccagagg gcaatctacc gctcgttccc
    16201 gaataccatt ctggaacacg acgtggtcat ccacggcgtt cgggttcgcg ccatcaccaa
    16261 cagcccgcac ctgtacgact tttgggtcga caactggtac tcggtccgcg agtgggaaac
    16321 cctgaccggt caggttccgc cgcacgaacc gaagattatg gtctacgctc tgggcggcgt
    16381 cagtgaagag ccggaggccg cctactacag ccgtcagacg aacactatca tcttcttcaa
    16441 cacctcttac tatggccaat tgaagtcctg ggtcttgggc gcggtgggtc ggatcctagc
    16501 cgaagaggag gggattcatt cgattcacgg cgcctgcgtg gagcggaacg gcaagggaat
    16561 tctctacatc gcgcccacgg gaacgggaaa atcgacctct tcctacggtt tgatgaacgc
    16621 cggcaattgc cgctttcatt ccgatgattg ggtctacgtc cgttacgccg gccacctccg
    16681 cgacggcagt ctcttcgtcc cgatgcgcat tctggaagaa aaacgcaagg tggcggaagg
    16741 atttgccgtt taccgctggc tggaggccaa cgggaaaacc aaaagtgacg ctaccctcga
    16801 gggcgtcgat ctccagaaca aggtcaaacg catccgggta ggcgacctcg atttctcccg
    16861 gccgctggag gcctacgcct acacctcgga gaaagtcttc tacctccgct ccaatttagt
    16921 ggagagcttc cccgaggtgg cttaccacat cctgcactct aagatcgaaa acgggccgga
    16981 tgctactctt gatttcgtcg ccgaccagaa aagcaaaatc gaaggcctct atgcccagct
    17041 ggaacgggcg cagaacccga ccgtacggcg atactttgcg caaattgatc ccgcccgcgt
    17101 caaggaaaca ctggcgcggc tctatgcgtt cgataacacc cgtgccatgc tggatattgc
    17161 ccgggttttt ggtcccggga acatcttcgc taatccctta gagccggtcc acctcaccac
    17221 ggtgatgctg ctcaagcgcg atttcggcga cccgctggtg ctcgactggc tgtcgctgga
    17281 cgagtttatg acctggcttt taatcggcga aacgcccgat aaaaagcgcg aaatcgcctt
    17341 caacgcttac cgcgcggtgg acgatgagac cgagaaaaaa ttcctcgtca aagtccagga
    17401 ggccaacggc ggcggaatcg accctaattt ctacggcacg tacgtcagcc agcccaacat
    17461 tcctcccacc ctgttgcagg agtttgaact tttccgggtg atgtaccagg ccacgaactg
    17521 ctacgtgatg aatacggttt tacagaagga tccaaagatc aaagggaaac aggaggcggt
    17581 gcagcgcagc atggccttga tcgcccggac catcgaccgg cagccaaaag atgtgcactt
    17641 aacactggag aattacgtag actttttagc gtgaaaaatg gacatccgaa aaaggaggga
    17701 atatgggacg aacactctcg ttcggcctga cagttctggc actgttcatt gtatcggcca
    17761 ccgctcagga gaaaaaggaa atggcgaaac cggttgaacc aaccggcttg aaagtcggcg
    17821 accccgcacc cgacttcacg ctgccctatg cgacgaagga cacgctggta tttcagggcg
    17881 ggatcaaact ttccgacttc aaaggcaaaa aaaacgtgct ggtgg
//
LOCUS       DBNGZA010000424         1300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_720347, whole genome shotgun sequence.
ACCESSION   DBNGZA010000424 DBNGZA010000000
VERSION     DBNGZA010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_720347"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..608
                     /locus_tag="AC16FU_09155"
     CDS             120..608
                     /locus_tag="AC16FU_09155"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR2994574.1"
                     /translation="MSSANLFAYGTLMDQSILAEQLGLSLEEVKQHFRFAKAELSGYR
                     RIFDVYRPQWDGLVLNLTPDPKATVVGILISGLSEKEPVQIDRIEGLSHERQEMTVRV
                     GNQLFLALVYVGRTSDTFHPIAEAYEKKILTIVDHLGGEIADNYYRLTLDPKGQPRYK
                     EK"
     gene            915..>1300
                     /locus_tag="AC16FU_09160"
     CDS             915..>1300
                     /locus_tag="AC16FU_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR2994575.1"
                     /translation="MEPKTVYLAGAVRTPIGKFGGALATLSAADVGTVAARAALSRAG
                     IKPEQVDEVIFGLARQAGCGPNPARQIGHRAGAPDTVPAFTVNKACGSGLKSIILAYQ
                     AILLGDAEIVLAGGMESMSNVPYFLP"
BASE COUNT          272 a          402 c          359 g          267 t
ORIGIN      
        1 tcttgccttt tctcgttggt gacgcgctga aagtccttgc cgcttccggt ttgtttcgcc
       61 tttaccaccg ccacgctaaa aaacttttcc cgcgctaaga tgaaaagagc tcagcccctg
      121 tgagctccgc caacctattc gcctacggaa ccttgatgga tcaatccatc ctggcggaac
      181 agctggggct gtcgttggag gaagtgaagc aacacttccg ctttgccaaa gcggaacttt
      241 ccggctaccg gcgcattttc gacgtttacc gaccgcagtg ggacggtttg gtgctcaacc
      301 tcacccccga cccgaaggct accgtcgtag ggattctcat ctctggtctt tcggaaaaag
      361 aaccggtaca gattgaccga atcgaagggt tgtcccacga aaggcaggaa atgaccgtgc
      421 gggtgggcaa tcaacttttt cttgcgcttg tgtacgtggg gcggacatcc gacactttcc
      481 acccgatcgc tgaagcctac gaaaagaaaa tcctgacgat cgtcgaccac ctcgggggcg
      541 aaatcgccga caattactac cgactgaccc tcgaccccaa aggccagccg cgctacaagg
      601 agaaataggg cgacttttag cgcggcgagg gcgccgcgcc tacgttggaa cgtcaaactc
      661 gcgtagggcg accgccctcg gtcccccacg agaatgcttg agataaccgg gctcgtcgcc
      721 ggaccggaca ccgaccactc agcgacgatc tggagaccgt cggtacaaac ttttgccttc
      781 gaccggctcg gagacgagcc cgactacctt ggacaaaatt tcagtacttt tttcacggaa
      841 ccggagagac cgggggagtc ctgcccggcg aaagacttta gcctcccgca acttttttac
      901 tatcttcccc cctaatggaa ccgaagacag tttacctggc cggggccgtt cgcactccca
      961 ttggcaaatt tggcggagcg ctggctacac tcagtgcggc ggatgtggga acggtcgcgg
     1021 cccgcgccgc actctcccgc gcggggatca aacctgaaca ggttgatgaa gtgattttcg
     1081 gcctggcccg ccaggccggc tgtggaccaa accccgcccg ccagatcggc caccgagccg
     1141 gcgcgccgga taccgttccg gcctttacgg tcaacaaagc ctgcggctcc gggttgaagt
     1201 cgatcatcct ggcctatcag gccattctct tgggggatgc cgagattgtc ctggccgggg
     1261 gcatggaaag tatgtcgaac gtcccctact ttttgcccaa
//
LOCUS       DBNGZA010000425         6763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_830717, whole genome shotgun sequence.
ACCESSION   DBNGZA010000425 DBNGZA010000000
VERSION     DBNGZA010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_830717"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC16FU_09165"
     CDS             complement(<1..216)
                     /locus_tag="AC16FU_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994576.1"
                     /translation="MKRSRDIAWLIRYCKSEPNCYRYLRKKRWRSLGPFCLKCRSPQL
                     WIYPEPSGRNNYICRKCRFHFNDLTASP"
     gene            819..2018
                     /locus_tag="AC16FU_09170"
     CDS             819..2018
                     /locus_tag="AC16FU_09170"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR2994577.1"
                     /translation="MPRSPEALIEPSLLIWARKSAGFSTKDVAKKLNVKLEKVEAWES
                     GKARPSVAQLRRLCPIYKRPLAVFFLPEPPRDFMPVRDYRRLADLSSPDEISADLRSE
                     IRRILELRDAAISLLEDIEERKLPELPIVGRYTDNPDELAARIRKALAISSRVQSEWT
                     DEFTALREWRNAAEGLGVLVTQASGIELDEMRGFSVADSQLPLICLNAADRANGRIFT
                     LMHELTHVVLREGGLCEWEHPRRRSPENKQAEAFCNRVAGAVLVPEEDLLQDPTVRGT
                     SGPRAWSDEEVRLLAQRFSVSREVILRRLLIVGRATDTFYQRRREALLKEYKLLGKRK
                     PFPVAYEKRIANALGRSFIQLVLTSYHERRLTLDDTSSLIGVRLKHLPAIEQEVMGWS
                     HIRMAGS"
     gene            2015..2473
                     /locus_tag="AC16FU_09175"
     CDS             2015..2473
                     /locus_tag="AC16FU_09175"
                     /inference="COORDINATES: protein motif:HMM:NF025725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HIR2994578.1"
                     /translation="MTYCIDTSALLVAWNERYPIINVPNFWNLFDQWVSQGKIISPIE
                     VRNEIKKKEDGLADWLKNREAMFVELEEDIQRAAREILVQFPLLTKGIEGRKIADPFV
                     ISLAKARGCTVVTEEGPGSPNRPRIPLVCRHFRTPCVNLLGLIQGENWVI"
     gene            2701..4188
                     /locus_tag="AC16FU_09180"
     CDS             2701..4188
                     /locus_tag="AC16FU_09180"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR2994579.1"
                     /translation="MSESYYNKIVSVQGKAAKMVGAALGLLILILFLWFGASPVKATV
                     PDGFVDTVIVSGLNQPVGFAFLPDGRILFIEQVTRRIRLVVQGAIAARDPLFTIADVN
                     TSGGERGLLGIAVDPGWPARPYIYCYFSHAGTATNYLVMFPVTGDLNDPTSDSLIISG
                     ASRYNILTDMPDNANNHNGGTLRFGSDGRLYLSLGEDAVPCAAQDSSGLRGMILRLSV
                     DSLPGAGAGPAPKSSITPSNNPFVASTNVNARLCYCFGLRNPFRFNIDPPTGDLFIAD
                     VGQNTWEEVSLSENGGENFGWPFREGPAVMIPGGCAEPGGPGAATYTAPIAYYNRTGF
                     TASIISGGIYRPKNYPNDFSFPPEYTGDGFYADYYQGFLRRIKKSGAVWITPAPVPGQ
                     PNANDWATGLQSPSDFQVGPDGGLYYISQFVSFTPNTGSLHRIGHRPTLPVLSGWGMA
                     AFGGTILFVFWMMWRRKTNLTTETQSETRLFERTRRNTKTVSNFG"
     gene            4241..4975
                     /locus_tag="AC16FU_09185"
     CDS             4241..4975
                     /locus_tag="AC16FU_09185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR2994580.1"
                     /translation="MQSVAGKVAIITGASRGIGKATALLFTQQKAKLALCARSKNDLN
                     KVAKDAEKVGAKCYLESVDVREEGEVNRFVSETQKRFGAIDILINNAGVGLYKPVAKT
                     TAEDWDWVLDVNLKGVFLFSRAVIPIMQAQQSGQIVNVVSGAGRTGIPNLAAYCASKF
                     GLLGFGEALALELRPYHIKVASILPGSVDTEWSAEFPKDRPKPNPLPVKLTLEEVAEG
                     ILFLCTQNENAWTSELNLRPHKVVAQ"
     gene            4987..5406
                     /locus_tag="AC16FU_09190"
     CDS             4987..5406
                     /locus_tag="AC16FU_09190"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR2994581.1"
                     /translation="MRKGMIGGLVLACGMVGTALAQEDTAKAMPAAAEVTVGHIVFGT
                     GIENREATGVDSSFAGDVGRVYCWTLITGASGETEVSHIWYHNDKEVAKVALPVRSPR
                     WRTYSSKTILPSTSGSWRVDVADASGNVLKSAKFEVK"
     gene            5566..6039
                     /locus_tag="AC16FU_09195"
     CDS             5566..6039
                     /locus_tag="AC16FU_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994582.1"
                     /translation="MKKVGLALSAVCFLSFLSTAVYSQGILQSQKPDLLRSPLQSGQG
                     LSLFAPSRFSFRNSYTLSFMSNGRQGNLLGLYVGSVNYQVAKPLEVTVNLGYLHQPQN
                     FVARNRQSGINGQILPSIRLDFRPSENFFFRIDVGSYPAWGGTPNLYDPYYFWNR"
     gene            6257..6733
                     /locus_tag="AC16FU_09200"
     CDS             6257..6733
                     /locus_tag="AC16FU_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR2994583.1"
                     /translation="MRSSRKTFWTKIAFGVLFIITVAYFASFVKRIAQGESSDAPYPV
                     RQARLQVLNGTGRPGLARKVADLYRRSTYQGVFFDVVDEGNSAPRVVEKTLVVNRTPE
                     DLWGEATAAVLGVASPQVLTHPLTDNVLNLHVTVIVGGDIDSLLRVQAQKKEGRAS"
BASE COUNT         1451 a         1804 c         2015 g         1493 t
ORIGIN      
        1 gggtgaggcg gtcaggtcgt tgaagtggaa gcggcacttg cggcagatgt agttgtttcg
       61 tcccgaaggt tcaggataaa tccagagttg cgggctgcgg cacttgaggc agaaggggcc
      121 gagcgagcgc cagcgttttt tccgaagata gcgatagcag ttgggttcgc ttttacagta
      181 gcggatgagc caggcgatat cgcgggaacg cttcataatt ccaccacaga ggcacggagg
      241 acacggagaa aaccgtcaac gggtttggaa aaggatagag ggaaaaagat agatctcacc
      301 ctaccctctc ctacaggaga gggggagttc gattcgcttt cttttgccct gggttttcca
      361 gggtggcccc ggggccgtag tcctgcgaga gcggagtgat gaccggcgtg cactggcgct
      421 tgaggcatat tctttagcgg agctcccgcc tgaaggaggc cagtcccgta gaggccggtg
      481 cagagcactc aggccctcta ctatttcgca ctttgtccgt tttacccccg tagtgacccc
      541 aaattctgag aaatggggga ttagggatca gagatcaggg attagaaggg cgaaaaagag
      601 ggcttcagat agagagaaat aaaaaaaggg cggttcacaa atcaccccta cttgcttgat
      661 ttatgtttcg tgataaattg aaaatcaaaa gagaagtcat gggtgaagtg ccgccagttt
      721 cgaagcgcga ccaacgccta accaggcgat gcagcgcact ggctagcgcc gaccgctaat
      781 cgccaggccg ttagcaggca taggagaact tagcgagaat gccgcgaagc cccgaagctc
      841 tcatcgaacc gagccttctc atctgggcac gtaagtccgc agggttttcg accaaggacg
      901 ttgccaagaa gctcaatgtc aaacttgaga aggttgaggc gtgggagagc ggtaaagccc
      961 gtcccagtgt cgcccagctg cggcgtctct gcccgatcta taagcgtcct ctggcggttt
     1021 tcttccttcc ggaacccccg cgcgacttca tgcccgtacg tgattaccga aggcttgcag
     1081 atttgtcctc tccggatgag atctcggctg atttgaggag cgagatccga cgcatacttg
     1141 aactgcgtga cgcagcgatt tcccttcttg aggatataga agagaggaaa ttacccgagc
     1201 tcccgatcgt cggtcgatat acagacaacc cggatgaact agctgctcgg atccgaaagg
     1261 ccctcgcaat ttcttccaga gtccagtcgg agtggactga cgagttcact gcgctccgcg
     1321 agtggcgtaa tgccgcagaa ggtcttggtg tacttgtaac tcaagcctct ggaatagagc
     1381 ttgacgagat gcgtggcttc tcggtggccg attcacagct tccgctcatc tgtctgaatg
     1441 cggccgatag ggccaacggt cgcatcttta ccttaatgca cgagctgact cacgttgttc
     1501 tacgggaagg cggtctgtgt gagtgggaac acccgcgacg gcggagcccc gagaacaagc
     1561 aggctgaggc attctgcaat cgagtcgcgg gagccgttct ggtgccggaa gaggatctcc
     1621 tccaagaccc aactgttcgt ggaacctcag gtccacgggc atggtccgat gaggaagtgc
     1681 gactcctggc ccagcgtttt tctgtgagcc gtgaggtaat tcttcgccgg ctcctcattg
     1741 taggccgcgc aacagatacc ttctatcagc gtaggcgcga agcgttgctt aaagagtaca
     1801 agttgctcgg caagcgcaag ccgtttccag ttgcctatga aaagcgcatc gcaaacgcgc
     1861 ttgggcgttc gttcattcaa ctcgttctta cgtcatacca cgagcgccgc ctaacgcttg
     1921 acgatacctc gagcctcatc ggcgtgcggc tcaagcacct cccagctatc gagcaggaag
     1981 taatgggttg gtctcacatc aggatggcgg gttcatgaca tactgtatcg acacgagcgc
     2041 tttacttgtc gcatggaacg agcgctatcc cataataaac gtacctaact tttggaatct
     2101 atttgatcaa tgggtttccc aagggaaaat cattagtccc atcgaagtgc gtaacgagat
     2161 caagaagaaa gaagacggtc tggctgattg gctcaaaaac cgcgaagcaa tgttcgttga
     2221 actcgaagag gatatccagc gggcagctag ggaaattctc gttcagtttc ctttgttaac
     2281 caagggtatt gaggggcgaa agattgctga ccctttcgtg atctccttag ccaaagctcg
     2341 agggtgtact gtcgttaccg aggaaggtcc tgggagtcca aatcgaccgc gcattccctt
     2401 ggtatgccgg catttccgga ctccttgcgt caacctgttg ggtcttattc agggggaaaa
     2461 ctgggttatc tagttttcgg gccgccggtc cttaggcgct tgtgggatag acgctcgatt
     2521 cacatgcgcg ggatacatgc ttttcgtcta gataagcata catgaataaa atacgcgggt
     2581 tgtgctttga ccctcccttc atccctccgc tcaaggagag gggaaaaata accctaaatg
     2641 tgaagtagaa tttgagtcct gcctaaaaac tcgtatactt ctcgcaagag tcgaccacgt
     2701 atgagcgagt cttactacaa caagatagta tcggttcaag gaaaagcggc taagatggtg
     2761 ggtgcggcgc tgggattgct cattctgatt ttatttctct ggtttggcgc atccccggtt
     2821 aaagccactg tgccggatgg atttgtggat acggtcatcg tgagcgggct aaaccagccg
     2881 gtggggtttg cgtttctgcc ggatgggaga atccttttca tcgagcaggt gaccagaaga
     2941 atccggttag tggtgcaggg ggccatcgcg gcgcgggatc cgctcttcac catcgccgac
     3001 gtgaatacct ccggcggcga gcgtgggctt ttggggattg cggtcgatcc gggctggccg
     3061 gcacggccgt acatctactg ctacttcagc cacgccggga cggccaccaa ttacctcgtg
     3121 atgttccccg taacggggga cctcaacgat ccaacgagcg acagtctcat catctcggga
     3181 gccagccggt ataacattct caccgatatg ccggacaatg ccaataacca caacggcggc
     3241 accctgcgtt ttggctccga tgggcggctg tacctcagtc tgggcgagga tgcggtgccc
     3301 tgcgcggccc aggattcgtc gggtctccgc gggatgattt tgcgtctttc ggtcgattcc
     3361 ctgccgggag cgggggcagg cccggcgccg aaatcgagca tcacgccttc gaataatcct
     3421 ttcgtcgcct ccaccaatgt gaacgcgcgg ctctgctact gtttcggcct gcgcaatccg
     3481 ttccgcttca atatcgaccc gcccaccggc gacctcttca ttgccgacgt gggtcagaat
     3541 acctgggagg aggtatcgct ctccgaaaac ggtggtgaaa atttcgggtg gccgtttcgg
     3601 gaaggcccgg cggtgatgat cccgggcggc tgcgcggagc cgggcgggcc gggagcggcg
     3661 acctacaccg cccccatcgc ctattacaac cggaccggtt tcaccgcgtc aatcatcagc
     3721 ggcgggattt atcggcccaa aaactatccg aatgattttt cctttccgcc cgagtacacc
     3781 ggcgacggct tttacgccga ctactatcag ggatttctgc ggcggattaa gaaaagcggg
     3841 gcggtctgga ttaccccggc acccgtcccc ggccagccga atgcgaatga ttgggccacc
     3901 gggctgcagt cgccgtccga ttttcaggtc gggccggacg gagggctgta ctacatctcc
     3961 cagtttgtca gttttacgcc gaataccggt tcgctgcacc ggatcggaca ccggccaacg
     4021 ttgccggtgc tttccgggtg gggaatggcg gcgtttgggg gtactatttt gttcgtcttc
     4081 tggatgatgt ggaggagaaa aacaaatctc accacggaga cacagtcgga gactcgactc
     4141 ttcgagagga cacggagaaa tacaaagacc gtatctaatt ttgggtaaaa ttcaacttcc
     4201 ctctttgagt ttaaacgtca cattctattt attgtaagcg atgcagtcgg tagcgggcaa
     4261 agtagccatc atcaccgggg cgtcgcgggg gatcgggaaa gcgacggcgc ttctttttac
     4321 ccagcaaaag gcgaagttgg cgctctgcgc ccggtcaaaa aacgatttga ataaagtcgc
     4381 gaaagatgcc gaaaaggtag gtgcgaagtg ctacttggag agcgtcgatg tccgggaaga
     4441 aggagaggtc aaccgcttcg tctccgagac gcagaagcgc ttcggcgcca tcgacatcct
     4501 gattaacaac gccggcgtcg gactgtataa gccagttgcg aagacgaccg cagaggactg
     4561 ggattgggtt ttagacgtta atctcaaagg tgtttttctc ttttcgcggg cggtcattcc
     4621 gataatgcag gcgcagcaaa gcgggcaaat cgtcaatgtc gtctccggcg cgggaaggac
     4681 cggcattccc aatttagcgg cctactgcgc gtcaaagttc gggcttttgg ggttcgggga
     4741 ggcgctggcg ctggagttgc ggccatatca tattaaggta gcgtcgattt tacccggttc
     4801 ggtcgatacg gagtggtcgg cggaatttcc caaagaccgg cccaagccga accccttgcc
     4861 ggtgaaattg acgctggagg aagtggccga agggattctg ttcctctgca cgcaaaacga
     4921 aaatgcctgg acgtcagaat tgaatttgag accgcacaag gtggtggcgc agtgaaggga
     4981 gggtttatgc ggaagggaat gatcggggga ctcgttttgg cctgcgggat ggtcggaacg
     5041 gccttggcgc aggaagacac tgccaaggcc atgccagccg cggctgaagt caccgtaggg
     5101 cacatcgtct tcggcaccgg catcgaaaac cgggaggcaa ccggagtcga ctcgtcgttt
     5161 gcgggagatg ttggtcgggt ctactgctgg actttgatca ccggcgcctc cggcgaaacc
     5221 gaagtgagcc acatctggta tcacaacgac aaggaagtgg cgaaggtggc tttaccggtg
     5281 cgctcgcccc gctggcggac ttattcgtcc aaaaccattc tgccgtcgac ctccggctcc
     5341 tggcgggtgg atgtggcgga cgcctcgggc aacgtgctca agagcgccaa gtttgaggtc
     5401 aagtaattcg cctcaggcgg gattcaggcc gcccggcgac cggccgaaat agagattaag
     5461 gattgacaga gcgtggtctt tgacattatt tgtgccaccg gtgctgccgg gtttgcgtat
     5521 atttaaagta aagacgagta gtagccctct ttcgaggagc gtggaatgaa aaaagtcggt
     5581 ttggcactca gcgcagtttg ctttttgagt tttctcagca cggcggtcta ctctcaggga
     5641 attttacagt cgcaaaaacc cgacctgctg cgttcgccgt tgcagtccgg gcaggggttg
     5701 tcgctttttg ccccgtcacg gttctcgttc cgcaacagtt ataccctctc gtttatgtcc
     5761 aacgggaggc aggggaacct tttggggctt tacgtcggtt cggtgaacta ccaggtcgcc
     5821 aagccgctgg aggttacggt caacttgggc tacctgcacc agccgcagaa ttttgtcgcc
     5881 cgtaatcggc agtcgggcat taacggacaa attctcccct cgatccggct cgattttcgg
     5941 ccgtcggaga atttcttttt ccggattgac gtcggcagtt accccgcctg gggcgggacg
     6001 ccgaaccttt acgatccgta ctacttctgg aaccgctaaa agcgaaggag caggccaaga
     6061 cggaccaaaa gtcaccacgc acccggggct gccaaatcca ctcctgccat cgcgcccatc
     6121 gtctgttgtc gtttagcact ctgttcgctg catatttgtc tcgactcaat ttccgcccgg
     6181 aggacttccg gagcgaatga ttcccctttt gcgccggaaa gctgcttccc cgcgccgggc
     6241 cttcagacgc cggacggtga gaagttcccg aaaaaccttt tggacgaaaa tcgctttcgg
     6301 ggtgctcttc atcatcacgg tggcctactt cgcttcgttt gtcaaaagga tcgcgcaggg
     6361 tgagtccagc gatgcgccct acccggtgcg gcaggcgcgg ctgcaggtgt tgaacggtac
     6421 aggccgcccg ggcctggccc ggaaggtggc cgacctgtac cgccgctcca cctaccaggg
     6481 ggttttcttc gacgtggtgg acgaaggcaa ctcggcgccc cgcgtcgtcg aaaaaactct
     6541 ggtggtgaac cgcacgccgg aagatctctg gggcgaagcc acggccgcgg ttctgggcgt
     6601 tgcctccccc caggtactca cccacccttt gacggacaac gttttgaacc tgcacgtgac
     6661 ggtgatcgtc ggcggagaca tcgattcgct cctgcgtgtg caagcgcaga aaaaggaggg
     6721 gcgtgcatcg tagtccggtg acaagatccg gccggcgtac cgc
//
LOCUS       DBNGZA010000426         8936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2843205, whole genome shotgun sequence.
ACCESSION   DBNGZA010000426 DBNGZA010000000
VERSION     DBNGZA010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2843205"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..787)
                     /locus_tag="AC16FU_09205"
     CDS             complement(131..787)
                     /locus_tag="AC16FU_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2994584.1"
                     /translation="MHIDPHTFKSFGRNAASGEAIYQQGEPAEKLFGLVSGQVKLVRT
                     VCGKSLTVALVGPGEFFGAVEFLTHRPFLCTARAEVAAQIVAVNRPFVESYLLAHPKV
                     MHRLLVQLAHQQALLIRQIENLLSTDIAYSAINTLVGLVPSQDGAPLPHSYPELVEVI
                     AHQVRTEKEKVEDALSFLVRSGMLEVVDDQIRALKRQEIEHYLVFLGQRLQFVGLEPA
                     "
     gene            complement(915..1925)
                     /gene="lipA"
                     /locus_tag="AC16FU_09210"
     CDS             complement(915..1925)
                     /gene="lipA"
                     /locus_tag="AC16FU_09210"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR2994585.1"
                     /translation="MEVAEVTSNAHDITLISRGTQRHPYFVHPEYAQPGQAVRVARKP
                     TWLKVTPPLGQNYRRLKAMLGGMKLHTVCQEANCPNIGHCYEKGTATFMILGDLCTRG
                     CRFCDVKRGKPGPVDPEEPKRLAEAAGKMGLRHVVITSVTRDDLSDGGASQFAACIRE
                     TRKTLPRSTIEVLISDLRGSREALEIVLAACPDVLNHNVETVPRLYTRVRLGAMYNRS
                     LELLARSKEIAPHILSKSGIMLGLGERRPEIEKTLWDLRGAGVDILTIGQYLSPSAWH
                     LPVEEYVHPDVFEELRVFGLSLGFREVYSGPLVRSSFNADEQLERAIGALPVTNGHLP
                     SE"
     gene            complement(2051..3034)
                     /gene="trxB"
                     /locus_tag="AC16FU_09215"
     CDS             complement(2051..3034)
                     /gene="trxB"
                     /locus_tag="AC16FU_09215"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR2994586.1"
                     /translation="MAKDKIYDVVIVGGGPAGLCAGLYTSRANLDVVLLEKLLPGGEI
                     TNTEWVEDYPGFDLIGGPELAKKMEEHAKKFGLKIEMQNVTEIYSEGLIKVVKTDDGE
                     YRSRTVIFCPGGSPNKLEIPGEKELAGKGVSYCAICDGAFFKSQVIAVVGGGDAAVEE
                     GTFLTKFGSKVYLIHRRDRFRAQKIIQERAFANPKIEVIWNTVVEEISGDSKVEHLRL
                     KNVATGEKKRLDVGAVFIFVGFVPNSHLIKDRVEKDKNGYVVTNDRMETNVPGLFVCG
                     DVRAQLCRQVTNAVGDATTAAVAAEKYLVKLKDAERSGRPVGQGQPLAVTK"
     gene            complement(3187..5013)
                     /gene="glmS"
                     /locus_tag="AC16FU_09220"
     CDS             complement(3187..5013)
                     /gene="glmS"
                     /locus_tag="AC16FU_09220"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR2994587.1"
                     /translation="MCGIVGYVGEQKAVPLLLEGLKRLEYRGYDSAGIAILENGELFS
                     TKAAGKIAVLEKVLNGREFAATSGIAHTRWATHGEPTDLNAHPHVDCKDEIAVIHNGI
                     IENFAALKAVLERQGHKFTSETDTEVLAHLIEEHYEGNLLEATRAALSLVEGTYGIAV
                     ISRNHPGEIVAARHGSPLLVGIGAGENFVSSDAAAILRHTKNVVYLEEKQIAHITRTS
                     YAISTIDNVSITPHVQEISWSLDMIEKEGFDHFMQKEIFEQSTTLRNAMRGRLNPPEG
                     TARLNGLLLRYDKLPQINRVIITACGTSWHAALIGEYLIEELARIPVEVEYASEFRYR
                     SPILNDGTVVLVISQSGETADTLAAMREAKHRGAPVFGICNVVGSTIARETDGGVYIH
                     AGAEIGVASTKAFTAQVMVLSLIALMLGRMRSLPPEKGKELLAGLQQVPELVQRILNT
                     DQKIKEIAQKLYQKNNFLYLGRGINFPVALEGALKLKEISYIHAEGYPAAEMKHGPIA
                     LIDENMPVVVIALKDAVYDKVISNIQEIRARRGRVIAIATEGDADIARLADFVISIPP
                     VADPLTPMLSVIPLQLLAYHMAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(5093..6457)
                     /gene="glmM"
                     /locus_tag="AC16FU_09225"
     CDS             complement(5093..6457)
                     /gene="glmM"
                     /locus_tag="AC16FU_09225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR2994588.1"
                     /translation="MNSQLKKQSSQLMVSVSGVRGVVGETLTPEVIVRYGLAFGQFVK
                     RKKVVIGRDARPSGPMVQNLAAGALLSTGCDVVDIGLTTTPTLELAVTDLKASGGVMV
                     TASHNPAEWNALKFFNASGTFLTPQQTKGVFDRAKKMHPGVKWNWAGKLSFDASRNAK
                     HIEQILGLPLINAFAIQKRRFRVVVDCVNGSGGVVVPELLARLGCEVTPLFCEPTGLF
                     PHNPEPVPKHLTALAQKVKETGADLGFAVDPDVDRLALVTEKGDAISEEYTLALAADL
                     VLSKTPGRVVVNLSTSRMVEDVAKKHGMPCYRTPVGEANVVLGLKKNKGVVGGEGNGG
                     VILPALHYGRDALVGMALILQLLVERQKTLNQLVSELPAYQMEKIKKPLPAGFEKRLK
                     KLALAFDGTRPDWRDGLRLDLPEGWLQVRKSNTEPVCRIVAEGQSEKSVNRILKEAQK
                     VLQS"
     gene            6654..7979
                     /locus_tag="AC16FU_09230"
     CDS             6654..7979
                     /locus_tag="AC16FU_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR2994589.1"
                     /translation="MKIKRSLSTVLCLVAASLLLAQAQEKKASPPGEGTHEVYLLTLE
                     GAITPVTAKLITQAIDKAEEKQAEALVIQLNTPGGLVLSTWQITKDILNADVPVIVYV
                     APSGARAASAGMYITYAAHIAAMASGTNIGAAHPVELQGGKADSTMMEKATNDAVANV
                     KSIATKRHRNEKWAEDAVRKSVSIPEYEALKLNVIDLVADNLSDLLAKCDGRTAELPI
                     GPRTLKTKGAPIRKVTIGPIDRILEVIVDPTIAYILFTIGMLGLYFEFANPGAIVPGV
                     VGGICLILAFYAFQSLPINYAGVLLIVLAMVLFILEVKVPSHGALTIGGIVAMLLGSL
                     LLITTSAPYARISLSVIITTVGATAAFFIFAVGAGLRAQRLKVTTGWEGMVGAIGVAH
                     SRFEPAQGGEGQIFVSGEIWRARASEPIEPNHKVKVTGVENLTLIVTRA"
     gene            8058..8819
                     /locus_tag="AC16FU_09235"
     CDS             8058..8819
                     /locus_tag="AC16FU_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR2994590.1"
                     /translation="MLPVPLLTIVLIGIFILANAIRILREYERGIIFRLGRLRGAKGP
                     GIIFLIPIVDKMVKVSLRVVAMDVPSQDVITRDNVSVKVNAVIYFRVIDASKAIVAVE
                     DFLYATSQISQTTLRSVLGQFELDDLLANREKINHQLQQIIDGQTEPWGIKVSVVEVK
                     NVDLPQEMQRAIAKQAEAERERRAKVIHAEGELQASYKLAEAAEVIGKQPAALQLRFL
                     QTLVEVAADKNSTTVFPVPIDLIKPFMSLAERGGK"
BASE COUNT         1873 a         2646 c         2464 g         1953 t
ORIGIN      
        1 cctgagcggg aggggtcggg gacgagcccg actaccaggt agacgcggca ctgagcgcct
       61 ttcgccgtgc gctccggctg aacctttctc tccaacattc cgataaaatc tacgataccg
      121 ctgacccgcc tcaggcgggc tccaacccca caaactggag ccgctgtccc agaaaaacga
      181 ggtagtgttc gatttcctgc cgcttgagtg cccgaatctg gtcgtcaacc acttccagca
      241 tcccggagcg cactaaaaag gagagagcgt cttcgacttt ttctttttcg gtgcgcacct
      301 ggtgcgcaat cacctcgact aattcgggat aggagtgcgg gaggggtgct ccgtcctggc
      361 tgggaacgag ccccaccagc gtgttgatgg cgctgtaggc gatgtcggtg gagaggagat
      421 tttcgatctg ccggatcaaa agcgcctgct ggtgggccaa ctgcactaaa agccggtgca
      481 ttaccttggg gtgcgctaag aggtaacttt ccacaaaggg acgattgacc gcaacaattt
      541 gcgcggcaac ttccgcacgg gcggtgcaca agaatggccg gtgggtgagg aactcgacgg
      601 ccccgaaaaa ctccccggga ccgacgaggg ccacggtcag acttttgccg cagaccgtgc
      661 ggaccaattt cacctggccg gaaacgaggc cgaacagttt ttccgccggc tcgccctgct
      721 ggtagatggc ttcccccgaa gctgcgttcc gaccaaagga cttgaaagtg tgcgggtcaa
      781 tgtgcacgat ttctttcccg cgtggtcatt ccctcctgcg caggcaccaa gattttcgcc
      841 gtgcttgcga cgcaaggtaa caacatccgg tcgccccctg caactcctct gtattttgaa
      901 gagttaccga tttactattc gctgggaagg tggccgttcg taaccggaag agcaccgatc
      961 gcccgctcca actgctcatc tgcgttgaag gaactgcgta cgagcggccc ggagtaaacc
     1021 tcgcggaaac ccagcgagag cccgaagacc cgcaattctt cgaaaacatc ggggtggacg
     1081 tattcttcca ccggcaaatg ccaggccgag ggggacaggt actgaccgat ggtgaggatg
     1141 tcgacgcccg cgccgcgcaa gtcccagagt gtcttttcga tctccggacg cctttcgccc
     1201 aaaccgagca taatgccgga cttggacaga atgtgcggag cgatctcttt tgagcgagcc
     1261 agaagttcca gcgagcggtt gtacatggcc cccaaacgga cgcgcgtata gagacgcggc
     1321 acggtttcaa cgttgtgatt caaaacgtcc ggacaggctg ccaacacgat ttctaatgcc
     1381 tcgcgcgagc cgcgcaggtc ggaaatcaaa acttcgatgg tgctccgggg aagcgtcttg
     1441 cgcgtttccc ggatacaagc ggcaaactgg gaagctccgc cgtccgatag atcatcacgg
     1501 gtgaccgagg taatcaccac gtgccgcaaa cccattttgc cggcggcttc ggccaatcgc
     1561 ttgggctctt ccggatcgac cggcccgggc ttgccgcgct tgacgtcgca gaagcggcag
     1621 ccgcgggtgc agagatcgcc cagaatcatg aaggtggcgg ttcctttttc gtagcagtgg
     1681 ccgatgttgg ggcagttggc ctcctggcag acggtatgga gcttcatccc ccccagcatc
     1741 gctttgagac gccggtagtt ctgtcccaga ggcggcgtga ctttcagcca cgtcggcttc
     1801 cgggccaccc gcaccgcctg ccccggctgc gcatactcgg gatggacgaa ataggggtgg
     1861 cgctgagtgc cgcgcgagat gagcgtgatg tcgtgggcgt tgcttgtgac ttcagcgact
     1921 tccatgcgag attcgaggcg cgacttaagt cgcgctgtag accgatttct atcgcaacct
     1981 gtggcgcagg ttggtcttag aaacctgcgt ccgtgcggga taccaagacc atcccgcact
     2041 tcgagctgat ttattttgtc accgctaaag gctgtccctg cccgacgggt cgtcccgacc
     2101 gctccgcgtc tttcaacttc accagatact tttccgccgc caccgccgcg gtggtcgcat
     2161 ctccaaccgc attggtcacc tggcggcaaa gttgggcgcg cacatccccg cagacgaaca
     2221 ggccgggaac attcgtttcc atccgatcgt tggtgacgac gtagccgttc ttgtcctttt
     2281 ccactctgtc tttgatcaag tggctgttag gcacgaaacc gacgaaaatg aaaaccgcgc
     2341 cgacgtccag ccgcttcttt tcaccggtcg cgacgttttt caaccgcaaa tgctctacct
     2401 tgctgtcgcc tgaaatctct tcgacaacgg tgttccaaat cacctcaatc tttggattcg
     2461 caaaggcgcg ttcctggatg atcttctgcg cccggaaccg gtcccgccgg tggatcaggt
     2521 agactttcga gccaaacttg gttaaaaacg tcccctcttc caccgccgcg tcgcccccgc
     2581 ctacgaccgc aatcacctga cttttgaaga atgccccgtc gcagatggcg cagtacgaca
     2641 ctcctttgcc ggccaactct ttttctcccg gaatttccaa tttattgggc gagccgcccg
     2701 gacagaaaat caccgtgcgc gagcggtatt ctccatcgtc ggttttgacc actttgatca
     2761 agccttcgga gtaaatctca gtcacgtttt gcatctcgat tttcagcccg aactttttgg
     2821 catgctcctc catcttcttc gccaattccg gcccgccgat caggtcaaag cccgggtaat
     2881 cttccaccca ctcggtgttg gtgatttccc cgccggggag gagtttttct aaaagaacga
     2941 cgtccaagtt ggcccgggag gtgtagagcc cggcgcagag cccggccggc ccgccaccca
     3001 cgatcaccac gtcgtaaatt ttgtctttgg ccatcggcta accttcagca agaaagaccg
     3061 ttgtatacgg tctttcatcg ataatacgtg ccgccgttgg aaaaagtcaa tccggcttga
     3121 agctctcccg accggcattg aaccggactc cctgattcct attgtttccg cgcgttccga
     3181 aatgatctat tccaccgtta cgctcttggc gaggttgcgc ggctgatcga cgttgcaccc
     3241 gcgcaggacc gccatgtggt aggccaagag ctgcagcgga atgaccgaga gcatcggcgt
     3301 caggggatcg gccaccggcg gaatggaaat gacgaagtcg gccaggcggg caatgtcggc
     3361 atcgccttca gtggcaatgg caatcacccg cccccggcgg gccctgatct cctgaatgtt
     3421 ggagatgacc ttgtcgtaga ctgcgtcttt gagcgcgatt accaccaccg gcatattctc
     3481 gtcgatcaaa gcaatcgggc cgtgtttcat ctccgccgcc gggtaccctt cggcgtggat
     3541 gtaggagatt tctttgagtt tcaaagcacc ttccagagcc actgggaaat tgatcccccg
     3601 gcctaagtag aggaagttat ttttttggta gagtttctgg gctatctctt taatcttctg
     3661 gtcggtgttg agaatccgtt gaaccaactc cggcacctgc tggagtccgg cgagcaattc
     3721 tttccctttt tccgggggaa gcgagcgcat ccggcccagc atcagggcaa tcagggacaa
     3781 taccatcacc tgggcggtaa aggccttggt cgaggccacc ccgatttccg ccccggcgtg
     3841 gatgtaaacg ccgccgtcag tctcccgggc aatggtggag ccgaccacgt tgcagatgcc
     3901 gaagaccggc gcaccccggt gcttggcttc ccgcatcgcc gccagcgtat ccgccgtctc
     3961 gccggattgg ctgatcacca ggaccaccgt gccgtcattg agaatcggac tccggtaacg
     4021 gaattcggat gcatactcca cctccaccgg aatccgggcc aactcttcaa tcagatattc
     4081 gccgatcaaa gccgcatgcc aggaggtccc gcaggcggtg atgatgacac gattgatttg
     4141 aggcaactta tcgtaacgta gcaataggcc gttcagccgg gcggtgccct cggggggatt
     4201 caagcggccc cgcatggcgt tgcgcagggt ggtcgactgc tcaaagatct ccttttgcat
     4261 gaagtggtcg aacccttctt tttcgatcat gtccagagac caggaaatct cctgcacgtg
     4321 cggcgtgatc gatacgttgt cgatggtgga aatggcgtag gaagtccggg tgatgtgggc
     4381 gatctgcttc tcttcgaggt aaaccacgtt tttggtatgc cgcaggatgg ccgcggcatc
     4441 cgaggagacg aagttttcac ccgcgccgat cccgaccaga aggggcgagc cgtgccgggc
     4501 ggcgacgatt tctcccggat gattccgcga aatgacggca atgccgtagg tgccctcaac
     4561 gagcgagagg gccgcccggg tggcctcgag cagattccct tcgtagtgct cttcaattaa
     4621 gtgcgctaag acttcagtat cggtctcgga ggtgaacttg tgcccctggc gttcgaggac
     4681 agctttgagt gccgcaaagt tctcgatgat cccgttgtgg ataacggcga tctcgtcttt
     4741 gcaatccacg tgcgggtggg cgttgaggtc ggtcggctcg ccgtgggtag cccagcgggt
     4801 atgggcgatg ccggaggtgg cggcaaattc gcgtccattc aggacttttt cgagaacggc
     4861 gattttgcct gccgctttcg tggaaaaaag ttcaccattt tccaggatgg ctatcccggc
     4921 cgaatcgtac ccccggtact ccagccgttt caggccctct aaaaggagcg gaacggcttt
     4981 ctgttcacct acgtaaccga ctatgccgca catggttagc tctaacctgc ttgccggaga
     5041 taatccgagc aaaccctaat cagttgccaa cattaaaata atctctcact cattaagatt
     5101 gcaagacttt ttgtgcctct tttagaattc tattcaccga cttttctgat tgaccttccg
     5161 caacgatgcg acagaccggc tcggtattcg acttccgcac ctgcagccac ccctcgggca
     5221 ggtcgagccg gaggccatcc cgccagtcgg gccgggtgcc gtcaaaggcc aaagccagct
     5281 ttttaagccg tttctcaaag ccagcgggca acggtttttt gatcttctcc atttgatatg
     5341 cgggtagctc acttactaac tggtttaagg tcttctgccg ctcgactaag agttgcaaaa
     5401 tcaacgccat ccccactaac gcatcccgtc cgtaatgtag cgcaggcagg atgaccccgc
     5461 cgttcccctc gcccccgacc acgcctttat tttttttcag cccgaggacg acattggctt
     5521 cgcccaccgg cgtccggtag cagggcattc cgtgcttttt tgccacgtct tccaccattc
     5581 gggaggtgga gaggttgacc accacccggc cgggcgtttt ggagagcacc aaatccgccg
     5641 ccagcgccag ggtgtactct tcgcttatgg cgtccccttt ttcggtcacc agcgccaggc
     5701 ggtcaacgtc cggatcaacg gcaaaaccta aatcggcccc ggtctctttg actttttgag
     5761 ccaaagcggt caaatgtttt ggcaccggct ccgggttatg ggggaaaaga ccggtcggct
     5821 cgcagaaaag aggggtcacc tcacatccta atcgtgccag aagttcgggg actacaactc
     5881 cgcctgaacc attgacacag tcaactacca cccgaaagcg ccgcttctgg atggcaaagg
     5941 cattgatgag cggcaggccg agaatttgtt caatgtgctt ggcattccgg gaggcatcaa
     6001 acgagagttt gcctgcccag ttccacttga cgccgggatg catcttcttc gctcgatcaa
     6061 aaactccttt tgtctgctgg ggtgtcaaga acgtgcccga agcattgaaa aacttgaggg
     6121 cattccattc cgccggattg tggctggcgg tgaccatcac cccgccggaa gccttcaaat
     6181 ctgtgacggc caactccaac gtcggagtgg tggtcaaacc gatgtccacc acatcgcagc
     6241 ccgtggaaag gagcgcgccg gcggctaagt tctgcaccat cggacctgag ggccgcgcat
     6301 cccggccgat tacgactttc tttcgcttta caaattgacc aaaggccaaa ccgtaccgga
     6361 caataacttc gggagtcagg gtttccccga ctaccccgcg cacgccggaa acactcacca
     6421 tgagctggga tgactgcttt ttcaattgag agttcatcta ttatgccatc gacagcgagc
     6481 cggtcggcct gtatcctcgg caaaacatat tgatttcttt cagcgtaagc aaaagaagtc
     6541 cggcaggcgg gtataattga cacgccctgc ttttggcgtt actttatctt ctgtcagtct
     6601 atccatttcg gagcgattac gtatagttcg cacggaatgg gaggatgcca gcaatgaaaa
     6661 tcaagagatc tctttctacg gtactctgtc tggtggcggc ctctttgctt ctggcgcagg
     6721 cccaggagaa aaaagcatct cccccgggcg aaggcacaca cgaggtgtac cttctgacac
     6781 tggaaggcgc cattacgccg gttaccgcca agttaatcac tcaggccatc gataaagccg
     6841 aagaaaagca ggccgaagcg ctggtgattc aactcaacac cccgggcggg ctggtgctct
     6901 ccacctggca gatcaccaaa gacatcttga acgccgacgt gccggtgata gtctacgtag
     6961 ccccttcggg cgcccgggcc gcttcggcgg gaatgtacat tacctatgcg gcccacatcg
     7021 ccgccatggc ttcggggacg aacatcggcg ccgcccaccc ggtggaacta cagggcggca
     7081 aggccgactc taccatgatg gaaaaagcca ctaacgacgc ggtggcgaac gtcaagagca
     7141 tcgccaccaa acgccaccgc aacgaaaaat gggccgagga tgccgtccgc aaatcggtct
     7201 caatacctga atacgaagct ttgaaattga atgtaatcga cctggttgct gataacttgt
     7261 ccgaccttct ggccaagtgc gacggccgca cggcggaatt gcctatcggc ccacgcaccc
     7321 tgaaaaccaa aggcgcgccc atacgcaagg tgaccatcgg ccccattgac cgtatcctcg
     7381 aagtgattgt tgatcccacc atcgcctaca ttctcttcac catcggaatg ctggggctct
     7441 actttgagtt tgccaatccc ggcgccattg tgcccggggt ggtgggtggg atttgcttga
     7501 ttctggcttt ctacgccttc cagagtttgc ccatcaacta cgccggtgtg ctgcttatcg
     7561 ttctggctat ggtgctcttt attctcgaag tcaaagtccc cagccacggg gctttgacca
     7621 tcgggggaat tgtggccatg cttttgggat cgcttctgct gattactacg tcggctccct
     7681 acgcccggat ttcgctttcg gtgattatca ccacggtcgg cgccaccgcc gctttcttca
     7741 ttttcgccgt gggtgccgga ttgcgcgccc aacggctgaa agttaccacc ggttgggagg
     7801 gaatggttgg tgcaattggc gtggcacatt caagatttga gccggcccag ggcggcgaag
     7861 gacagatttt cgtttccggg gaaatctggc gcgcccgagc cagcgaaccg attgagccca
     7921 accataaggt taaggtaacc ggagtagaaa acctgacgct gattgtaacg cgtgcctgag
     7981 tcacgcattc gaactggcaa aacgaaacag acgataactt ggtactgaaa acttcttaaa
     8041 cccatcacgg aggaacaatg cttcccgtcc cgttattaac gattgtcctg atcggcatct
     8101 tcatcctcgc caatgccatc cgcatcctgc gcgagtacga acgcggtatt atctttcggc
     8161 tggggcgctt gcgcggagcc aaaggcccgg gaattatttt tctgatcccc atagtcgata
     8221 agatggtcaa agtctccctg cgggtggtgg ccatggacgt tccctcccag gacgtaatca
     8281 cccgggataa cgtttcggtc aaggtcaacg cggtgattta cttccgggtg attgacgcct
     8341 ccaaagccat cgtggcggtg gaggacttcc tctacgccac ctcccagatt tcgcagacca
     8401 cgttgcgttc cgtactggga caatttgaac tcgacgacct tctggccaac cgggaaaaaa
     8461 tcaaccacca gttgcagcaa atcatcgacg gccagaccga accctggggc attaaagtct
     8521 ccgtagtaga agtaaagaac gtcgatctgc cccaggaaat gcagcgcgcg attgccaagc
     8581 aggccgaggc ggagcgcgag cgccgcgcca aggtcatcca cgccgaaggc gaactccagg
     8641 cctcctacaa attggccgaa gcggccgagg tgatcggcaa gcagcccgcc gccctgcaac
     8701 tgcgcttttt gcaaacttta gttgaagttg ccgccgacaa gaattcgacg acggttttcc
     8761 ccgtccccat cgacctgatc aaaccgttca tgtcattggc ggaacgggga gggaagtagc
     8821 ccgaaacccg cgcatctgac ttggtaaccc agccacttgt gggcgtaaaa cagaggagga
     8881 cacgatgccc aagtacgtga ttgaacgtga tattcccggc gccggaaaac tatccg
//
LOCUS       DBNGZA010000427         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1466531, whole genome shotgun sequence.
ACCESSION   DBNGZA010000427 DBNGZA010000000
VERSION     DBNGZA010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1466531"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="AC16FU_09240"
     CDS             complement(<1..601)
                     /locus_tag="AC16FU_09240"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR2994591.1"
                     /translation="MSLRNLKVAIIGLGQIGGSLGRDLVFGKVVKEVIGSDRDRVTRR
                     WSMRTKAVHRVADSLSGAVRSADLVVLAVPVRRIVSLLPQVAQHLKPGAVVFDVGSTK
                     REIVRAMERLPRGILACGGHPMAGTEKSGIRAAQKNLFTDKAFFLVPTRKTKPESLAL
                     CRRLVRAVGARPVVISAAAHDRLLATTSHLPYLLAVALLE"
     gene            complement(598..972)
                     /gene="aroH"
                     /locus_tag="AC16FU_09245"
     CDS             complement(598..972)
                     /gene="aroH"
                     /locus_tag="AC16FU_09245"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR2994592.1"
                     /translation="MVRGIRGAVTVKENSRRAIFAATQKLLRAMVRDNHVTRDDIASV
                     FLTATEDLNVEFPAYALRQMGWKTVPILCAREMGVPGSMRKVIRALIHVNMDKAQPEI
                     KHQYLGGAKKLRPDLFRRSGRK"
     gene            complement(1190..>2011)
                     /locus_tag="AC16FU_09250"
     CDS             complement(1190..>2011)
                     /locus_tag="AC16FU_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR2994593.1"
                     /translation="DRIALAMIEEAEKKGLIRPGATIIEPTSGNTGISLALVCAVKGY
                     KMIAVMPGAMSSERAAMMKLLGARVEIVPTRDNVPGLFTKEDIDNTIRRAQEIQAETP
                     GAYVPNQFENPDNPHIHALTTAREIIDQTVGKFQAFVAACGTGGTFCGVAAVLKEQFP
                     QIRRVVVEPKESAVIAGGQPGPHKIQGIGEGFIPKVMDVTLVDEAIGVSDADAILTAK
                     RLAAEEGVLTGLSGGANVFAALEVGKKMKKGEVVVTIIPDNSLRYLSTELCRCQE"
BASE COUNT          386 a          620 c          559 g          446 t
ORIGIN      
        1 tctccaaaag cgccaccgcc agaagatagg gcaggtggct ggttgtggcc aacagccggt
       61 cgtgcgcggc agccgaaatg actaccggac gggcgccgac agcgcggacc aatcgacggc
      121 acagcgcgag tgattcgggt ttggtcttcc gggtcggcac caggaaaaat gctttgtcgg
      181 taaaaagatt tttctgtgcc gctctaatcc ccgatttttc cgtccccgcc atcgggtgtc
      241 cgccacaggc cagtatcccg cgcgggagac gctccattgc ccggacaatt tcccttttcg
      301 tgctcccgac gtcaaagaca acggctccgg gcttcaggtg ttgggcgacc tgcggtaaaa
      361 gggaaacgat ccggcgcacg ggtacggcga gaacgaccag atcggcggac ctgaccgcac
      421 cggagagcga gtcggcaacc cgatggactg cttttgtacg catcgaccaa cggcgtgtaa
      481 cgcggtcgcg atcggaaccg atcacttcct tgactacttt cccgaagacc agatctcgcc
      541 ccagcgaacc gccgatctgg cctaagccga tgatggcgac cttaagattg cggaggctca
      601 ttttcgtccc gaccgccgga acaaatcggg ccggagtttt ttcgccccgc ctaggtactg
      661 gtgcttgatc tctggctgcg ctttgtccat gttgacgtgg attaaagccc gaatcacctt
      721 ccgcatgctg cccggtaccc ccatttcccg ggcgcagagg attggcaccg tcttccaccc
      781 catttgccgg agggcgtagg ccggaaattc cacgttcagg tcctccgtcg ccgtgaggaa
      841 gacgctggcg atgtcatctc tcgtaacgtg attgtcgcgc accatcgctc gcaagagttt
      901 ttgcgtcgcc gcaaaaatcg cccggcgtga attttccttg accgtcaccg cgccccgaat
      961 gcctctgacc attttgctcc tttcttgccg ttcccgaaac aaaaaagccc gctgtctttt
     1021 ccggcagtgg gctggtggcc gtcaaaaaca ataagtcacg aataacccac tggagtggga
     1081 agcgaataat aataccaatg tgcccggata tgaaagaacg gtctgtgcat cggtcgtttc
     1141 ttaataaaac agctacacgt agccgaaagt tccacgaccc gggccattct tattcctggc
     1201 accggcagag ttccgtagag agatagcgca ggctgttatc aggaataatc gttaccacga
     1261 cttcgccttt cttcatcttt tttccgacct cgagggctgc aaacacattt gcgccgccgg
     1321 aaaggccggt caggacccct tcctccgccg ccaggcgctt ggcggtgaga attgcgtctg
     1381 cgtcgcttac cccgattgcc tcatcgacca gggttacatc cattaccttg gggatgaacc
     1441 cttcgccgat gccctggatt ttgtgggggc cgggctgccc gccagcaatg accgctgact
     1501 cctttggttc gaccaccacc cgccggatct gcgggaactg ctctttgagg accgccgcca
     1561 cgccgcagaa ggtccctccc gttccgcagg ccgccacgaa ggcctggaat ttcccgacgg
     1621 tctggtcgat gatctcgcgg gcggtggtga gggcatgaat gtggggattg tccgggtttt
     1681 caaactggtt gggaacgtat gcgcccggcg tctctgcctg aatttcctgg gctctccgga
     1741 tggtgttatc gatgtcttcc ttggtgaaca agccgggtac gttgtcccgg gttgggacga
     1801 tctcgacccg cgcacccaaa agtttcatca tcgccgcccg ctccgagctc atggcgcccg
     1861 gcatcaccgc gatcattttg taccctttca ctgcgcaaac cagggccaga ctgattccgg
     1921 tatttcccga agttggctca atgatagttg cgccgggtcg gatcagcccc tttttctcgg
     1981 cttcttcaat catcgccaga gcgatccggt c
//
LOCUS       DBNGZA010000428         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2204940, whole genome shotgun sequence.
ACCESSION   DBNGZA010000428 DBNGZA010000000
VERSION     DBNGZA010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2204940"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>2748
                     /locus_tag="AC16FU_09255"
     CDS             175..>2748
                     /locus_tag="AC16FU_09255"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR2994594.1"
                     /translation="MAVLATIKTTPGGLLQEIELLCQAQKYRQAAELLAQISPEESGP
                     PNAKVAYLRALTFHHAGEYRQALAAARETLGALSVSSDHDLVAKTQYVIGDTYLALGD
                     LKNAELYLRDSVSHFRRVENHRGRAQGYNELARVAFIQADFPRALDMLSEALEACQLS
                     GDEMLRAKIMGNFGRVYTLMGRWEEAERYVLLGLLLNEHLGIEVSICRNLLSLGYLFF
                     LRGRWDEAAEQYQKALRLIEKNDFRREWAIYFEYAGDLAAAQGKTAEAERLYREGLAI
                     GEAIAPDSTITSQIGRRLAELSLAQGNLSLAADQCQRALDLAATIGEKSEIGACYRVL
                     GAIAGLQGEPGQAKSYLAHALTVLDECGDRFELVKTCLAACAFVLEHLSDLSLTHWAV
                     QVLSQGRDTARALGLREETARFTLFEIEYLCRREEFDRAQKLLQSTAGEISSAGLSVK
                     TDFDALVARLNRALVEHGQSPRNSFRFLSFVNGNGAQAAGASPGELFRFAAERLQAHY
                     GDLREAQEGRLGQPLVVVGILPDETEAQTGCDLSLPLLVNCYSGNGVNRLEIPLRSGD
                     AVLGRIQFVREASLFTQEEIDFACATAELLALKLLEESHQKVLDDNRRLREALQSTCA
                     FPNVITAHAGFTEMLKRLDQVKDSTLSLLLEGETGTGKDLLAKAIHYSSARRDKPFVS
                     VNCAALPETLLESELFGHRKGAYTGADRDKIGLFAEADGGTFYLDEVSELPPSIQVKL
                     LRVLEEKEVTRLGDTRPQKVDVRVISAGNKDLKTQMEKGLFRADLYYRLCTLTFKLPP
                     LRERKEDIPLLVDHFVKKHSPAAGTTLPRIDPAFLRILTEYDWPGNVRELENEIRKVL
                     LL"
BASE COUNT          569 a          833 c          817 g          529 t
ORIGIN      
        1 tgacacctta gccatcacga atgagtacct gattctgcgc atctggacca gtcccgattc
       61 gttttacgtc attaccgtac ctcggccgac ggcgcgtggc ctcactgact acggtaaaag
      121 aacgcgatca ccattagaac gttaaccttc ctctttaagg gagtagagaa gaggatggcc
      181 gttttagcca cgataaaaac aaccccgggc ggtctccttc aagagatcga gctcctctgt
      241 caggcgcaaa agtaccgcca ggcggctgag cttttggccc agatttctcc agaggaaagc
      301 ggccccccga acgcaaaagt ggcctatctg cgggcgttga cgttccacca cgcaggagag
      361 tatcgccagg ccctggctgc ggcgcgggaa acgctggggg ccttaagcgt cagcagcgat
      421 catgacctgg tagccaaaac ccagtacgtt attggcgaca cctacctggc gctgggcgac
      481 ctgaaaaacg ccgaactcta cctgagggat tcggtttccc atttccggcg ggtagagaac
      541 caccggggac gggctcaggg gtacaacgaa ctggcgcgcg tggcgttcat ccaggccgat
      601 ttcccgcgcg cgctcgacat gctctccgaa gccctggaag cctgccagct ctccggggac
      661 gaaatgctcc gggccaaaat tatgggaaac ttcggccgcg tttacaccct gatggggcgc
      721 tgggaggaag cggagcgcta tgtccttttg ggcttgctct tgaacgagca cctgggcatt
      781 gaggtgagca tctgtcgcaa cctgctctcg cttggctact tgttcttcct ccggggccgc
      841 tgggatgaag cggctgaaca gtatcagaaa gcgttacgat tgatcgagaa aaacgatttc
      901 cggcgggagt gggcgatcta cttcgagtat gcgggtgacc tcgccgccgc ccagggcaaa
      961 accgctgaag ccgaacggct ctaccgcgag ggactggcca tcggcgaggc cattgctccc
     1021 gacagcacga tcacgagcca gatcggccgg cggctggccg aactctctct ggcccagggt
     1081 aatctctctc tggctgcaga ccagtgccag cgggcgctcg atctggcggc gacgatcggc
     1141 gaaaagtcgg agatcggcgc ctgctaccgc gtattgggag ccattgccgg gcttcagggt
     1201 gagcccggcc aggccaaaag ctacctggcg catgcgctga cggtcttaga tgagtgcggc
     1261 gaccgtttcg aactggtcaa aacctgtctg gcggcgtgcg catttgtact ggaacatctg
     1321 tccgacctgt ccctaaccca ctgggccgtg caggtactct cgcagggacg cgacaccgcc
     1381 cgcgcactgg ggttgcgtga agagacggcg cgtttcacgc tgtttgaaat tgaatacctc
     1441 tgccgccggg aagagttcga tcgcgcccaa aagttattgc aaagcaccgc aggtgagatt
     1501 tcctcggccg gactgagcgt caaaacggat ttcgacgcat tggtggccag gctgaaccgt
     1561 gccctggtgg aacacggcca gtctccccgg aactctttcc gcttcctctc ctttgtgaat
     1621 ggcaacggtg cgcaagctgc cggcgcgtcg ccgggcgaat tgttccgctt tgccgccgaa
     1681 aggctgcaag cccactacgg cgacctgcgg gaagcccagg aaggccggct gggacaaccg
     1741 ctggttgtgg tcggaattct gcctgacgaa acggaagcgc aaacgggatg cgatctctcc
     1801 ctgcccctcc tggtgaattg ctactcgggc aacggggtaa accggctgga aattcccctc
     1861 cggtccgggg atgcggtcct gggccgtatc caatttgtgc gggaagcaag tctttttact
     1921 caagaggaga tcgacttcgc ctgcgccacg gccgagcttt tggcgctcaa actcctggag
     1981 gagtcgcacc agaaggttct ggacgacaac cgccggctcc gggaagcgct gcaatcgacc
     2041 tgcgctttcc ccaacgtcat caccgcccat gcgggattta ccgaaatgct caagcgtctc
     2101 gaccaggtga aagattccac cctgtcgctt ctcttggaag gcgaaaccgg caccggcaaa
     2161 gacctcctgg cgaaagcgat tcactactcg agcgccagac gggacaagcc gttcgtttcg
     2221 gtgaactgtg cggcgttgcc ggaaacgctt ctggagagcg agctcttcgg ccaccgcaaa
     2281 ggggcctaca ccggcgcgga tcgcgacaag atcggcctct ttgccgaggc cgacggcggc
     2341 accttctacc tggacgaagt ctccgaactg ccgccctcga ttcaggtgaa actgctgcgg
     2401 gttctggagg aaaaagaggt gacccggctg ggcgacaccc ggcctcagaa agtcgacgtg
     2461 cgggtcatct ccgccggcaa caaagacctg aagacccaga tggaaaaagg cctcttccgg
     2521 gccgacctct actaccgact ctgcacgctg accttcaaac ttccgcctct gcgcgaacgg
     2581 aaagaggaca tcccactttt agtcgatcat tttgtgaaaa agcactcccc cgccgctggc
     2641 acgactctcc cgcgcatcga ccctgccttt ctgcggattc tgacggaata cgactggccg
     2701 ggcaatgttc gcgaattaga gaatgaaatc cgcaaagtgc ttctgttg
//
LOCUS       DBNGZA010000429         8950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2843273, whole genome shotgun sequence.
ACCESSION   DBNGZA010000429 DBNGZA010000000
VERSION     DBNGZA010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2843273"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="AC16FU_09260"
     CDS             complement(<1..756)
                     /locus_tag="AC16FU_09260"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR2994595.1"
                     /translation="MQTLTQPAVPDLPVLPGYQVLERLGSGGMAIVYRGIQSALDRPV
                     AIKRLHTHLTPDPTFLERFAREAKAAASLHHEHIVSVIDFAQKPNGCYIVMEYVEGAT
                     LRALLRETTPLPLEIVLLILASVARGLAFAHDRGFVHRDIKPDNIMVGRDGSVKITDF
                     GIVKSNADTTLTRTGSVVGSPAYMAPELLQDLSVDSRADIFSFGVTAYEILAGAKPFR
                     GDSPSAVITSMLHHAPVPLLERNQEVSPALEDLI"
     gene            complement(967..1446)
                     /locus_tag="AC16FU_09265"
     CDS             complement(967..1446)
                     /locus_tag="AC16FU_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994596.1"
                     /translation="MKRITLAAMLTALLAQAAVTAFSAGAPGQVPATTPAAAPSIKLD
                     WYSVNGGGITLGSSATSKLGLSVGQSVAGVGTSASFKLGIGFWQGAGSFCAMLGDLSG
                     DGILDIVDLAAGVNYVVFGTPIPTGEVCGDLTGDGVIDITDLICLLNFVVFGNPIPC"
     gene            complement(1475..2851)
                     /locus_tag="AC16FU_09270"
     CDS             complement(1475..2851)
                     /locus_tag="AC16FU_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994597.1"
                     /translation="MRVRILLFTAALSLLATIAFAAQSSVPNLINFQGKLTDPAGNPV
                     TDGSHSANFSLWTLRTGGLSVWSEITTQTTAGGLFTHQLGSVSSLSPTLFQDYDSLFL
                     EIVADGQTITPRTKLTSVPYSRVAGGLEVGSASGFCAAPNQLAIETDASDHRISTYGC
                     DGQEQIRLWGVSWGEMLLHDQSAANVTTARLSAQPSFNPSGGGGELFLRNNPAGSVID
                     LYAGNTGDASVVLPTDAINSAEIKDEPGVANNDNTSFFNLNAGNIVYVVDSVDINIPA
                     AGYVEVTCGAWLNLFHTNLTTTEVWFEPGEARGSLGFPSPGGMVASVPSVTATSVWQF
                     PATSSRFFAATAGTHRYYLHARYAQGTNASTNVAAHYIRATYYPTLYGTATLATSVGG
                     TVSELSQVATDGSLRLPKTELATITVQDHNARLEAELAKMRAEMEARIQKLESQVNQS
                     QQVGENEK"
     gene            3201..5678
                     /gene="gyrA"
                     /locus_tag="AC16FU_09275"
     CDS             3201..5678
                     /gene="gyrA"
                     /locus_tag="AC16FU_09275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR2994598.1"
                     /translation="MPLEREKILPVFIEEEMKSSYIDYAMSVITARALPDVRDGLKPS
                     NRRISVAMNDLGLAPNRAHRKCAKIAGDTSGNYHPHGEQVVYPTLVRMAQDFNMRYPL
                     VDGQGNFGSIDGDAAAAMRYTEARLTPLAMEMLADLEKDTVDFQPNYDETRQEPKVLP
                     GKFPNLICNGSSGIAVGMATNIPPHNLSEVVDAVIAQIDNPDIDLDELLQHIKGPDFP
                     TGGIITGTRGIGEAYRTGKGRIVVRARATIERLKGDKNNIVVTEIPFQVNKSQLVERI
                     AELVREKVIEGISDLRDESDRDGMRIVVELKRDVVPEVVLNQLFKHTQMQETFGIINL
                     ALADGVPQVLPLKAILQNFIDHRHVVVTRRTQFDLKKAEERAHILEGYKIALDNLDAV
                     ITLIRKSQTPEEAREGLMKRFKLSQIQAQAILDLRLQRLTGLERKKIEEEYLETIKLI
                     EELKSILASKTKRLAIIKEELSELKQKYGDERRTEIREEEEEFSIEDLIAEEDMVITI
                     THSGYIKRLAVTAYRRQGRGGRGVIGIETKEEDFVEHLFIASTHEYLLFFTRAGRLYW
                     LKVHEIPTGGRLSKGKPIVNLLALSENEKIAAMVPVRRFDDQHFIVMATRNGVIKKTS
                     LDEFSNPRKGGIIAVTIPENEKDELIDVAVTDGTCDIVLATRQGQAIRFPEDKVRSMG
                     RTAYGVIGIELEKKDYLIGMVVLKRKATLLVVTEKGYGKRSDIDDYRVTNRGGKGVIN
                     VKVTDRVGEVIAIKEVLDSDELMIITQKGIVIRLPIKPIRVMGRNTQGVRLINLEEGD
                     KVTDVARVVTEGEETEQVVEGDGQAKA"
     gene            5773..6273
                     /locus_tag="AC16FU_09280"
     CDS             5773..6273
                     /locus_tag="AC16FU_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2994599.1"
                     /translation="MPQRKTRSKGTAKKAPAKTGKSIARTITQNCFIHAYPEVVFDAL
                     VNPLVLKRWFVEEAEVEPKKGGRYVWDWGKGGKLESKITEYQPPKKLVITFTGWNSGM
                     KEKCTFTLKPKEGGTLLHMEDTDHIAGSKGWERHISVVRGWAVALLNLKSVLESGKDL
                     RNAAAW"
     gene            6360..7355
                     /locus_tag="AC16FU_09285"
     CDS             6360..7355
                     /locus_tag="AC16FU_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR2994600.1"
                     /translation="MTLQGKKVLVTGAGGFIGSHLVERLVALGARVACFLRYNSRGLK
                     GWIDEFPADVQSQLTIFWGDLKDPEAVRLAVKGQEIIFHLGALIAIPFSYITPRDFVD
                     TNVLGTTNLLNAIREYGCERLIHTSTSEVYGTAQYVPIDEKHPLVGQSPYAASKIAAD
                     MLVQSYFRSFDLPAVTIRPFNAFGPRQSLRAIVPTIITQALAGKVVSLGSVHPTRDMN
                     YVADTVEGFIAIASCEGIEGETINIGSGQEVAIRNLVERVGKILGKTLEIKTEEKRVR
                     PEASEVARLAADNRKAKKLLGWQPKVSLDAGLNKTIEWFRAKRNLCATQEREYQV"
     gene            7352..8056
                     /locus_tag="AC16FU_09290"
     CDS             7352..8056
                     /locus_tag="AC16FU_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR2994601.1"
                     /translation="MRAVNMAGGKGTRLAPYTTVLPKPLLPIDSTPILEIILRQLKRA
                     GITRITLAVGHLGHLLENFFGDGQALGLSIDYHREKKPLGTAGPLVRIGSLDETFLVM
                     NGDILTDLDYRKLVAYHRRHKALATIGSYERKVPVDFGVIETNSSARLQGYREKPVLA
                     YDVSMGVYIFEPEVLKFIPKYRKFDFPELVWALLQRRKKVISYPYGGIWYDIGRPDDY
                     ARATAEFLRHRKRFFV"
     gene            8316..>8950
                     /locus_tag="AC16FU_09295"
     CDS             8316..>8950
                     /locus_tag="AC16FU_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994602.1"
                     /translation="MPEKYFYFIISDDAYYYFTIARNFVTRGMFSADGITLTNGFHPL
                     WLFIISPIYLVFRGSPWLSIHFAMTVAAIFDTGAGFFVYKSLEKLGKPTVGFWAACFY
                     LVNPYGLLHTMNGLETAQNNFFLTFLVYLSVRATPDWLQTRWFFLGTICGLTLLSRTD
                     NLFAVGVLLGYLFWRNRNFTVIAKATAAAGLLFAPWLIFNAVKFGSIVQTSG"
BASE COUNT         2014 a         2407 c         2657 g         1872 t
ORIGIN      
        1 gattaagtct tccagcgccg gggagacctc ctgatttctt tccaaaagtg gcaccggcgc
       61 gtggtgcagc atcgaggtga tgaccgcaga gggggaatcg ccccggaacg gtttggcccc
      121 ggctaaaatt tcgtaagccg tcacgccaaa agagaagata tcggcccggg agtccaccga
      181 caggtcctgc agcagctccg gcgccatgta ggccggactg ccgaccacgc tgccggtgcg
      241 ggtgagggtg gtatcggcgt tcgatttgac gatgccgaaa tcggtgattt tcaccgatcc
      301 gtcccggccc accatgatat tatccggctt gatgtcgcgg tggacgaacc cccggtcgtg
      361 ggcaaaggct aagcctcggg ccacactggc gaggatcaaa agcacaattt ccagcggcag
      421 gggggtcgtt tcgcgcaaca gcgcccggag ggttgccccc tcgacatatt ccatgacgat
      481 gtaacagccg ttcggtttct gggcgaaatc gatgaccgaa acgatgtgtt cgtggtggag
      541 cgaagccgct gctttggcct cgcgggcgaa gcgttccaaa aaggtcggat caggggtcag
      601 atgggtgtgg agtctcttta tcgccaccgg gcggtcgagg gcgctctgaa tcccccggta
      661 gacgatagcc atgccgccgg agccaaggcg ttccagcacc tggtagcccg gcaaaaccgg
      721 caggtccggc acagccggct gcgttaaggt ctgcacagag acatccatac ataatcgagt
      781 atcgtcaccc ctcagcggga cttaatgaaa gcgcccaggc cctttcgggc agattgatga
      841 ggaactcttt ggaaagtttc gggaaagcgt ttgtgaccga attggttgcg acggacaaag
      901 agggctcttt tccccaaaaa aagagcgccc aagcgggcgc tctttactgt gaccgtgcgc
      961 tttttctcag cagggtatcg ggttaccgaa aacgacgaag ttgagcagac agatgaggtc
     1021 ggtgatgtca atgacgccat cgccggtcag gtccccgcag acctccccgg tggggatggg
     1081 ggtgccgaag accacgtagt tgaccccagc agcaaggtcc acgatatcca gtatcccgtc
     1141 gcccgaaaga tcacccaaca tagcgcagaa cgagcccgct ccctgccaga agccgatgcc
     1201 gagcttgaag gaagccgaag tacccacccc cgccaccgac tgcccgacgg agagccccag
     1261 cttggaagtg gccgaggagc ccagagtgat cccgccgccg ttcacggaat accaatcgag
     1321 tttaatactc ggtgcagcag ccggggtagt cgccggcacc tgccccgggg caccggcaga
     1381 gaaagcagta accgctgcct gcgccaatag cgctgtgagc attgcagcca gagtgatgcg
     1441 cttcatctca ctcctccttg gaccggccgt cttattactt ttcattttcg cccacctgct
     1501 gggattggtt gacctgactt tccagcttct gtatgcgggc ctccatttcg gcccgcattt
     1561 tagccagttc cgcctccagg cgggcattgt ggtcctgcac ggtgatggtg gccagttcgg
     1621 tttttgggag ccgtagcgag ccgtctgtgg cgacctgtga gagttcggac acagtcccgc
     1681 caacagatgt ggctagagtt gccgtcccgt acagagttgg gtagtaggta gcccggatat
     1741 aatgggccgc tacatttgtc gacgcatttg tgccttgggc ataccttgcg tggaggtagt
     1801 accgatgagt cccggctgtt gccgcaaaga aacgactcga ggtggcggga aattgccaaa
     1861 cgctggtggc cgtgacggaa gggacacttg ctaccatacc gccgggcgaa ggaaaaccca
     1921 aactcccccg agcctcaccc ggctcgaacc atacttcggt agttgtgaga ttggtgtgga
     1981 acagattcag ccaggcccca caggtgacct caacataacc ggctgccggg atattgatat
     2041 cgacagaatc cacgacgtaa acgatattgc cggcgttcag gttgaaaaag ctggtgttat
     2101 cattattcgc cacccccggc tcgtccttga tctctgccga gttgatggcg tcggtcggaa
     2161 gcaccaccga cgcatctcct gtattccccg catacaggtc gatgaccgaa cccgccggat
     2221 tgttgcgcag gaacaattcg cctccgccgc cgctcgggtt gaaggatggt tgagccgaaa
     2281 gccgggccgt agtcacgttg gccgcgcttt ggtcgtgcaa aagcatttct ccccacgaaa
     2341 ctccccagag acggatttgc tcctgcccgt cgcagccgta ggtggaaatt cggtggtcgg
     2401 acgcatcggt ttcgatggcg agttgattgg gagcagcgca gaatccgctg gcgctaccca
     2461 cttccagccc gcccgctacg cggctgtagg gaacgctcgt tagcttggtc ctgggagtta
     2521 tggtttggcc gtcagccacg atttccagaa acagtgaatc gtagtcttga aagagcgtcg
     2581 gtgaaaggga gctcacggaa cccaactggt gggtgaacag tccgcccgcg gtggtctggg
     2641 ttgtgatttc gctccagacc gaaagtccac ctgtgcgcag cgtccagagg gaaaaattgg
     2701 cgctgtggga gccgtcagtg accgggttac cggcgggatc ggtcagtttc ccttggaaat
     2761 tgatcagatt ggggacagaa gactgggcag caaaggcgat tgtggccaaa agagacagtg
     2821 ccgcagtgaa aagcaaaatc cgtacgcgca ttctccacct ccatttgtgt gtaccgcgtt
     2881 gaaccatggg cgggtcgttg gcgacccttg ggactggtga acgtgctccg ctcgaatgcg
     2941 gattattctg agcccttacg ctgaaactct gcggtcgata accctcctct tcgttgtgcg
     3001 ttccctcttc aataccctcc gatgtacttt agcggaaaga aattgcggag gacgggacat
     3061 gtcaaggact ttctggcccg cacataagta attatgataa agaatatccg gctacgcatc
     3121 tggcatttct gggctcactc aagtatattc tctgaccggg attgatggct gccactaact
     3181 tgaaagataa aggataactt atgccgctgg aacgcgagaa aatacttccc gttttcattg
     3241 aagaggagat gaagtcgtcg tacatcgatt acgcgatgtc ggtgattacg gcgcgggcct
     3301 tgcccgacgt gcgcgacggg cttaagccct ccaaccgccg catctcggtg gcaatgaacg
     3361 acttggggct ggcgcccaac cgcgcccacc ggaagtgtgc caagattgcg ggcgacacct
     3421 ccgggaacta ccacccccac ggcgaacagg tggtctatcc cactctcgtt cggatggcgc
     3481 aggacttcaa catgcgctac ccgttggtgg acggccaggg gaacttcggt tccatcgacg
     3541 gcgatgcggc tgcggcgatg cggtacaccg aggcgcggtt gacgcctctg gcaatggaga
     3601 tgctggccga cttggaaaaa gacaccgtcg atttccagcc caactacgac gaaacccgcc
     3661 aggagccgaa agtcctcccc ggcaaattcc cgaacttaat ctgcaacggc tcctccggca
     3721 tcgccgtcgg catggcgacc aacatccccc cacacaacct ttccgaggtg gtggacgccg
     3781 tcatcgcgca aattgacaac cccgacatag acctcgatga attgttgcag catatcaaag
     3841 ggcccgattt ccccaccggc gggatcatca ccggcacgcg cggcatcggc gaggcctatc
     3901 gcaccggcaa agggcggatt gtggtccgcg cccgggccac tatcgagcgc ctgaaaggcg
     3961 acaagaacaa catcgtggtc accgaaattc cgttccaggt gaataaatcg cagttggtcg
     4021 aacggatcgc cgaactggtc cgggaaaagg tgatcgaagg catctccgac ctgcgcgacg
     4081 aatcggaccg ggacgggatg cggatcgtgg tcgaactgaa gcgcgacgtg gtgccggaag
     4141 tggtgctgaa ccagctcttc aagcacactc agatgcagga gaccttcggg atcatcaatc
     4201 tcgccctggc ggacggcgtc ccgcaggtgc tcccgctcaa ggcaattttg caaaacttca
     4261 tcgaccaccg gcacgtggtg gttacccgcc ggacccagtt cgacttaaag aaagccgaag
     4321 aacgggcaca cattctcgaa gggtacaaga ttgctctgga taatctggac gcggtcatca
     4381 ccttaatccg caaatcgcag acgccggagg aagcgaggga aggtttgatg aagcgcttca
     4441 aactttccca gatccaggcg caggcgatcc tcgatctgcg tttgcagcgg ctcaccggtt
     4501 tagagcgcaa aaaaattgaa gaagaatatc tcgaaacgat caagttaatc gaggaattga
     4561 agagcattct ggcctccaag accaagcggc tggcaatcat taaagaggaa ctttccgaac
     4621 tcaagcaaaa atacggcgac gagcgccgga ccgaaatccg cgaggaagag gaagaatttt
     4681 ccatcgaaga cctaatcgcc gaagaggaca tggtgatcac catcactcac tccggctaca
     4741 tcaaaaggct ggcggtgacg gcctaccggc ggcaggggcg gggcgggcgc ggggtgatcg
     4801 gcatcgagac caaagaggag gatttcgtcg agcacctctt catcgcctcc acccacgagt
     4861 acctcctctt tttcacgcgg gcggggcggc tctactggct caaagtgcac gaaattccca
     4921 ccggcgggcg gctctccaaa gggaaaccga tcgtcaacct cctggctttg agtgaaaacg
     4981 aaaaaatcgc cgccatggtg ccggtgcggc ggttcgacga ccagcatttc attgtcatgg
     5041 ccacccgcaa cggagtgatc aaaaagactt cgctggacga attctccaac ccgcgcaaag
     5101 gcggcatcat cgccgtcacc attcccgaaa acgaaaaaga cgagttgatc gacgtggcgg
     5161 tcaccgacgg cacctgcgac atcgttttgg ccacccggca ggggcaggcg atccgttttc
     5221 ccgaagacaa agtgcgctcc atgggccgca ccgcctacgg cgtcatcggc atcgaattgg
     5281 agaagaaaga ctacctcatc ggcatggtcg tgctcaagcg caaggcaacg cttttggtcg
     5341 ttaccgaaaa agggtacggc aagcgctccg acattgacga ctaccgggtg accaaccggg
     5401 gcggcaaagg ggtgatcaac gtcaaggtta ccgaccgggt gggcgaagtg atagccatca
     5461 aagaagtgct cgactccgac gaattgatga tcatcaccca aaaagggatc gtcatccggc
     5521 tgccgattaa gccgattcgg gtgatgggcc ggaacaccca gggggtgcga ctgatcaacc
     5581 tcgaagaagg cgacaaggtg accgacgtgg cgcgggtagt gaccgaaggc gaagaaaccg
     5641 agcaagtggt ggagggcgac ggccaggcga aggcctgaca acggattttt gaacggatta
     5701 aacggattga ctttggtagc atagcccttt gtgggcatct ggatcttttt tagaatattc
     5761 gttgagacat tcatgcccca gaggaaaact cgatctaaag ggacggcgaa gaaagctcca
     5821 gcaaaaacgg gaaaatcaat tgcccgcacc atcacccaaa actgcttcat ccacgcctac
     5881 ccggaagtgg tgttcgacgc gctggtaaac cctctggtgc tcaagcggtg gtttgtggag
     5941 gaggcagagg tcgagccgaa aaagggcggg cggtacgtct gggactgggg taagggtggc
     6001 aaattagaaa gcaaaatcac agaataccag ccgcccaaaa aactcgtcat caccttcacc
     6061 ggctggaata gcgggatgaa ggaaaaatgc acttttactc tcaagcctaa agagggcggg
     6121 acgctcctgc atatggaaga taccgaccac atcgccgggt ccaaaggttg ggaacggcac
     6181 atctccgtgg tgcgcgggtg ggcggttgcg cttttgaact tgaagtcggt tttagaatcg
     6241 ggcaaagacc tgcgcaatgc ggccgcctgg taagacccgc taatagtcgg ccgtaatctt
     6301 tgagggggcg cgtttcgatt caactcgatt ctaccgataa ttagaccagg atgagtggga
     6361 tcaccctgca gggcaaaaag gtgctcgtca cgggcgccgg cgggttcatc ggcagccatt
     6421 tagtcgagcg gctggtggct ctcggagcgc gggttgcctg ttttctgcgc tacaactcgc
     6481 gcgggctcaa agggtggatt gatgaattcc ccgccgacgt gcaaagtcag ctcaccatct
     6541 tctggggcga cctgaaagac ccggaggcgg tccggctggc ggtcaaggga caggagatca
     6601 tcttccacct cggtgctttg attgccattc ctttttccta tatcacgccg cgcgacttcg
     6661 tggatacgaa cgtgctggga acgacgaacc tgctcaatgc aattagagaa tatggctgtg
     6721 agcggttgat tcacacttcc acgagcgaag tgtacggcac ggcgcagtac gttcccatcg
     6781 atgaaaagca tcctttggtg ggacaatcgc cctacgcggc ctcgaaaatt gcggcggata
     6841 tgctcgtgca gagctacttt cgttctttcg acctgccggc ggtgaccatt cgtccgttca
     6901 atgctttcgg gccgcgccag tcgttgcggg caattgtgcc gaccatcatc acgcaggcgt
     6961 tggccggcaa agtcgtttcg ctggggtcgg ttcatccgac gcgggatatg aattacgtcg
     7021 ccgatacggt cgagggtttc attgcgattg catcgtgcga aggcatcgag ggcgagacga
     7081 tcaacatcgg ctccgggcag gaggttgcga ttcggaattt agtggagcga gtcgggaaaa
     7141 ttctcggtaa gactttggag ataaaaaccg aagagaagcg ggtgcggccg gaggcgagcg
     7201 aggtcgcgcg gctggcggcg gataatcgga aagcgaaaaa acttttgggg tggcagccga
     7261 aggtctccct tgatgcggga ctgaacaaaa caatcgagtg gttccgcgcg aagcgcaatc
     7321 tctgtgcaac gcaggagcgc gagtaccagg tatgagggcg gtcaacatgg cgggcgggaa
     7381 ggggacgcgg ctggctccgt acacgaccgt tttgcccaag ccgctcttgc cgattgattc
     7441 gacgccgatt ttagaaatca tcctccggca gttaaagcgg gcaggcatca cgcgcattac
     7501 gctcgcggtc gggcacctgg ggcatttgct ggagaatttt tttggagatg ggcaggcgct
     7561 gggactctcg attgactacc accgcgaaaa aaagcctttg gggacggcgg ggccgctggt
     7621 gcgaattggt agtcttgacg aaacgtttct ggtgatgaat ggcgacatcc tgacggatct
     7681 cgactaccgg aaattggtcg cgtaccaccg gcgacacaaa gcgctggcga ccatcggatc
     7741 gtacgagcga aaagtgccgg ttgatttcgg agtgattgag acgaatagca gcgctcgttt
     7801 acaaggatac cgggaaaagc cggtgctcgc gtacgacgtc agcatgggcg tttatatttt
     7861 tgaaccggaa gtgctcaagt tcatcccgaa gtaccgaaaa ttcgatttcc cggagttagt
     7921 gtgggcgctt ttgcagcgga ggaagaaagt gatttcatac ccgtacgggg gcatctggta
     7981 tgacatcggg cggccggatg actacgcgcg ggcgacggcg gagtttttga gacatcggaa
     8041 gcggtttttt gtatagataa agtcgtaggt ggaagatggt gggggactaa agaagccccc
     8101 cgaagttccg gggttgatgc ggctcgttcc gggaaggggc gcgggggaat ggagcggggg
     8161 tcagttgcaa aagcataaaa catattctcc tactatttcg cacactttaa tttcattctg
     8221 tttatattga attagtgaag cttgagagcc cgtctcaaag aaaggttttt tatctttttg
     8281 cttttggctt tctcatccgg atcgctctcg cctggttgcc cgagaagtat ttctatttca
     8341 tcatttctga cgatgcctac tactacttta ccatagccag aaattttgtt acgcggggaa
     8401 tgttttcagc ggacggcatt accctcacca acgggttcca tcctctctgg ttatttatca
     8461 tctcgccgat ttacctcgtt tttcgaggca gtccgtggct cagcattcat tttgcgatga
     8521 ccgtagcagc gatttttgat accggcgccg gtttctttgt ctacaagagt ctggaaaaac
     8581 tggggaaacc aaccgttggc ttttgggccg cgtgtttcta tctggtcaat ccctatggtt
     8641 tgttgcacac catgaatggc ctggaaaccg ctcagaacaa tttttttctg acttttctgg
     8701 tgtacctgtc tgtgcgcgct acacctgatt ggctccaaac gcgctggttt ttcttgggaa
     8761 ccatctgcgg tttgacgtta ctttcccgca ccgataactt attcgccgtt ggcgttctcc
     8821 ttggctatct tttttggcgc aatcggaact tcaccgtgat tgcgaaagcc actgccgcag
     8881 cgggcctgct ttttgcgcct tggttaattt ttaacgcggt gaagttcggt tcaatcgtgc
     8941 agacctcagg
//
LOCUS       DBNGZA010000430         2686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_831131, whole genome shotgun sequence.
ACCESSION   DBNGZA010000430 DBNGZA010000000
VERSION     DBNGZA010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_831131"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /gene="dapF"
                     /locus_tag="AC16FU_09300"
     CDS             complement(<1..545)
                     /gene="dapF"
                     /locus_tag="AC16FU_09300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR2994603.1"
                     /translation="MAEIEFVKMHGAGNDYIFIDTIRRPVRANFNRLARLVSDRHTGV
                     GGDGLVLLRRGRRYPFRMEIYNADGSQAEMCGNGIRCLARLIYEAGHATGRLQAIETC
                     GGIIRTEIISTGNGQRPFTVRVDMGKPELAVKRIPVRSPQKYFINQSVRIGSRKINLT
                     CLALGNPHAVTFVRALPENWAL"
     gene            complement(768..1181)
                     /gene="atpC"
                     /locus_tag="AC16FU_09305"
     CDS             complement(768..1181)
                     /gene="atpC"
                     /locus_tag="AC16FU_09305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR2994604.1"
                     /translation="MFELSIATPEKVFYEDQVESVTAPGADGYLGVLAHHAPLITTLL
                     PGKVIVRDKEKQTIVFAVSGGFLEVSRNKATLLADAVELAEQISVDRARRALARAQER
                     IHKKEPGIDLSRALEALKRAENRMRIHKESYPQRA"
     gene            complement(1314..>2686)
                     /gene="atpD"
                     /locus_tag="AC16FU_09310"
     CDS             complement(1314..>2686)
                     /gene="atpD"
                     /locus_tag="AC16FU_09310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR2994605.1"
                     /translation="PTVDCEFPSDRLPNMLSAIKIEDEKRGIKLTVEVALHIGDNVVR
                     CVALSSTDGLVRGMPALDTGGPITVPVGPQTLGRIFNLLGDPIDEQGPLPSGNKRYPI
                     HRPAPSFEEQETKIQLFETGIKVIDLLEPYPKGGKVGMFGGAGVGKTVILQELIHNIA
                     TEHGGYSVFSGVGERTREGNDLWLEMKASGVLAKTCMVFGQMNEPPGARLRVGLSGLT
                     MAEYFRDEEHQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLGTEMGALQERI
                     TSTKSGSITSVQAIYVPADDLTDPAPATTFSHLDATTVLSRQISELGIYPAVDPLTST
                     SRILDPKVVGEEHYDVARAVQKILQRYKDLQDIIAILGMDELSEDDKVIVARARKIQK
                     FLSQPFFVAEVYTGTQGRYVKLTDTIRGFKMIVEGQLDHLPEQAFYMLGQIEEAIERG
                     EQLLKR"
BASE COUNT          529 a          837 c          756 g          564 t
ORIGIN      
        1 agagcccaat tttccgggag tgcccggacg aaagtgaccg cgtgcggatt gccgagtgcc
       61 aaacaggtca aattgatttt ccgcgaacca atgcggaccg actgattgat aaagtatttc
      121 tgcggagagc gtaccggaat tctctttacg gccagttccg gcttccccat atccacccgc
      181 acggtaaagg gtctctggcc gttgccggtg gaaatgattt ccgtacggat gattccgccg
      241 cacgtctcaa tcgcctgcaa gcgaccggtc gcgtgcccgg cttcgtaaat gagccgcgcc
      301 aggcagcgaa tgccgttccc gcacatttcc gcctgcgagc catcggcatt gtaaatctcc
      361 atgcggaagg gataccttct cccccgccgc aagaggacga gcccatcccc gccgacgccg
      421 gtatgccggt cgctgaccaa ccgggccagg cggttgaaat tggcccgcac cggccggcgg
      481 atggtatcga tgaagatgta gtcgtttccc gcgccgtgca ttttcacaaa ctcgatttcg
      541 gccattcttc tgaatttaac gcattgctcc taaaaacaaa aagcccacta cttttccgac
      601 tgtagtgggc gcgcaagccg cgttttagcc ggttctcacc cactacttct gacagcatac
      661 caccacgccc agctaagacg ctgggagcgg aaagtcagaa gttgggccaa gaccggattc
      721 ataatctgca tacaagatat aacgactgcc gtatttgtca agtcctttca tgcccgttgg
      781 gggtaacttt ccttatgaat tcgcattcgg ttttccgccc gcttcagggc ttccaacgcg
      841 cgggagaggt caatccccgg ctcctttttg tggattcgct cctgcgcccg ggctaaggcc
      901 cgcctggccc ggtcaacgct gatttgttcc gccaactcca cggcatcggc caaaagcgtc
      961 gccttgttgc gggagacttc gagaaagccg ccggacaccg caaaaacaat cgtctgtttc
     1021 tctttgtcgc ggacgataac ctttccgggc aaaagcgtcg tgatgagcgg cgcgtggtgg
     1081 gccagaacgc ctaagtaacc gtccgcgccg ggagcggtga ccgattccac ttggtcttcg
     1141 tagaagactt tttcaggagt agcaattgaa agctcgaaca tcaccttttc aacctcgtcc
     1201 ggctcaggga cgagccggac taccgagcgc ccttgcgtgg cagtggtagc cgggctcgtc
     1261 cccaagcccg gctgtccgac aaatcggacg acttaatctc accaacagtt tggctaccgc
     1321 ttcagcagtt gttctccccg ctctatcgct tcctcaatct gccccagcat gtagaacgcc
     1381 tgctccggca ggtggtccaa ttgtccctcg acaatcatct tgaacccccg gatcgtgtcg
     1441 gtcagtttga cgtaccgtcc ctgggtgccg gtgtagacct ccgccacgaa gaagggctga
     1501 gagaggaatt tttgaatctt ccgcgcccgc gccacaatca ccttgtcgtc ttcggagagt
     1561 tcgtccatcc ctaaaatggc gatgatgtcc tggaggtctt tgtagcgctg gagaattttc
     1621 tgcaccgccc gcgccacgtc gtagtgttcc tcgcccacca ctttggggtc gagaatgcgc
     1681 gaggtcgagg tcaaaggatc gaccgccggg taaatgccca actcggaaat ctggcgcgag
     1741 agtacggtcg tggcatcgag gtgcgagaaa gtcgtcgccg gtgccgggtc ggtcaggtcg
     1801 tcggccggga cgtaaatcgc ctgcaccgag gtgatggaac ccgacttggt cgaggtaatc
     1861 cgctcctgca gcgcgcccat ctccgtcccc aaggtcggct ggtagccgac cgccgacggc
     1921 atccgcccca aaagcgcgga gacttcggag ccggcctgca cgaagcggaa gatgttatcg
     1981 ataaagagca gcacatcctg gtgctcctca tcccggaagt attcggccat ggtaaggccg
     2041 gaaagcccca cccgcagccg cgcgccgggc ggctcgttca tctggccgaa gaccatgcag
     2101 gttttggcga ggacgcccga ggctttcatc tccagccaga ggtcgttccc ttcgcgggtg
     2161 cgctccccca ccccggagaa aaccgagtag ccgccgtgct cggtggcgat gttgtgaatc
     2221 aactcctgca ggatgaccgt cttccccact cccgccccgc cgaacatccc caccttgccg
     2281 cccttggggt acggttcgag cagatcgatg acttttatgc cggtttcaaa caactgaatc
     2341 ttcgtttcct gttcttcgaa agacggcgcc gggcggtgaa tgggatagcg tttgtttccc
     2401 gacggaagcg gaccttgctc gtcaatggga tcgcccaaga gattgaaaat gcgtcccaga
     2461 gtttgcggcc caaccggcac cgtgatgggt ccgccggtgt ccagcgccgg catcccgcgc
     2521 acgagaccgt cggtggagga gagcgccacg cagcgcacaa cgttgtcgcc gatgtgcagc
     2581 gccacctcga ccgtcagctt aatgccgcgc ttttcgtctt cgattttgat ggcgctaagc
     2641 atattcggca gccggtccga gggaaactcg cagtccaccg tcggcc
//
LOCUS       DBNGZA010000431         2577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2843663, whole genome shotgun sequence.
ACCESSION   DBNGZA010000431 DBNGZA010000000
VERSION     DBNGZA010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2843663"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16FU_09315"
     CDS             <1..219
                     /locus_tag="AC16FU_09315"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR2994606.1"
                     /translation="EFIDRVKHERDPMITKAVSWLLREVAKVKPGETAAYLARNRATL
                     PALALRETENKLRTGLKSGKRKHSRHPY"
     gene            256..1077
                     /locus_tag="AC16FU_09320"
     CDS             256..1077
                     /locus_tag="AC16FU_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994607.1"
                     /translation="MRTAGRLGWAIEANWADPLLFAIYSIVRPIFSTLILVFMYKVIA
                     GGKTDTELFAFIYLGNAFFMYVTNLLSGMGAIVHDDREQYEMLKYIYVAPINKYAYLI
                     GRGLPKLFLTTLAVIITLTFGIFVFHLKIDWQAANWLLFLVAFPVGLFAALCLGLILA
                     AVCLNTARHGFALSEGSAGVFYLAIGAVFPIDVLPHWLQKVSLFIPLTYWLELLRRSL
                     GAGGLSQTLSRYSNGHLLWILFLTALGLALTAHLVYQWMEHLARKQGKLDLKFNY"
     gene            complement(1089..1835)
                     /locus_tag="AC16FU_09325"
     CDS             complement(1089..1835)
                     /locus_tag="AC16FU_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR2994608.1"
                     /translation="MGNLVSHFRRIEYRGKLFIFFVIWWAVWAINPVHPSDWRLENVL
                     TVLFIPILIATRNSFRLSNLSYTLIGIFLSLHTVGAHYTYAEVPYDRWFKTLFGTTVN
                     EIFGFQRNHFDRLVHFLFGLLMAYPIREVFVRIANVRGIWGYYLPLDLTMSLSMLYEL
                     VEWGASILFGGDLGAAYLGTQGDNWDAHKDMALATSGAVICMVVTALVNWRYQRDFAA
                     EMRDSLKVKGKTPLGEVKLREMREEGKGGR"
     gene            1962..>2577
                     /locus_tag="AC16FU_09330"
     CDS             1962..>2577
                     /locus_tag="AC16FU_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR2994609.1"
                     /translation="MDFLTHGLTGAALARFFYGKVRPLVAIITLVIGATMPDYDSVVR
                     FWSMTDFLEFHRGISHSLVGVTVAAIILASIMKAIFRDAGYLKLFVLALTGGLSHIFL
                     DMINSYGTQLFMPFTRYRYAWDLVMIIDPFITVSLILALALSVIFKRRRVLFCGIAIA
                     LILTYVGLRAVCHAIAVGTVEKFALPGEKVVASGAYPEILTPFEW"
BASE COUNT          548 a          742 c          653 g          634 t
ORIGIN      
        1 gaattcatcg accgcgtcaa gcacgagcgc gacccgatga ttaccaaggc ggtgtcgtgg
       61 ctcctgcgcg aagtggccaa agtaaagccc ggggaaacag cggcctatct cgcccgcaat
      121 cgcgccacct tgccagccct cgccctccgc gaaaccgaaa acaaattgag aacgggatta
      181 aagagcggaa aacgaaaaca tagtcggcac ccttactaaa ttctcaagat gcgactgaaa
      241 cccaatctca tgacgttgcg aaccgccggc cgcctcggct gggcgattga agccaactgg
      301 gccgatccct tgcttttcgc catctactcc atcgtccggc caattttctc gactttgatt
      361 ctggttttca tgtacaaagt cattgccggg ggaaagaccg acaccgaact ctttgctttc
      421 atctatctgg gcaacgcctt ctttatgtat gtgacgaatc tcttatccgg aatgggtgcc
      481 atcgtgcacg acgaccgcga gcagtacgaa atgctcaagt acatttatgt cgcaccgata
      541 aacaagtacg cttacttgat tggtaggggc ttgccgaaat tgtttctgac caccctcgcc
      601 gtcattatca ccctcacgtt cgggatcttc gtcttccatt tgaaaattga ctggcaggcg
      661 gccaactggc tcctcttttt ggtcgccttt ccggtcggcc tttttgcggc gctttgtctc
      721 ggcctgattt tagccgccgt ctgcctgaac acggcccgcc acggctttgc tctctccgag
      781 ggctccgccg gagttttcta cctagctatc ggcgcggttt ttccgattga cgttttgcct
      841 cactggctgc aaaaggtctc tttattcata ccgttgactt attggctgga acttttgcgc
      901 cgctcgttgg gagcaggggg actgagccaa actctttcgc ggtattcaaa tggccacttg
      961 ctttggattt tgtttttgac tgccctgggc ctcgctctca ccgcgcattt agtttatcag
     1021 tggatggaac acttagcccg caagcagggg aaattagatc tgaagtttaa ttactgaggc
     1081 cgaagccgtc accttccccc tttgccttcc tcccgcatct cccgcagttt cacttcaccc
     1141 aaaggcgttt tgcctttcac tttcagactg tcccgcattt cagcggcaaa atcgcgctgg
     1201 tagcgccagt tgacgagcgc ggtgacaacc atgcaaatca ccgctccgga cgtggccagc
     1261 gccatatcct tgtgcgcgtc ccagttatcc ccctgtgtgc cgaggtaggc cgcgcccaga
     1321 tcgccgccga agagaatcga cgcgccccat tcaaccaatt catagagcat cgaaagcgac
     1381 atcgtcagat ccagaggcag gtagtagccc cagatgcccc ggacgttggc gatccgcacg
     1441 aagacctcgc ggatgggata cgccatgagc agcccgaaga gaaaatgcac cagccggtcg
     1501 aagtggttgc gctgaaaacc gaagatttcg ttgaccgtcg ttccaaaaag cgttttgaac
     1561 cagcggtcgt acggtacctc ggcgtaggtg tagtgcgcgc cgacagtgtg cagggagagg
     1621 aaaataccaa tgagcgtgta cgacagattg ctcagccgaa aactgttgcg cgtggctatg
     1681 agaatcggaa tgaaaagtac cgtgaggacg ttctccagcc gccagtcgct ggggtgaacc
     1741 ggattgatgg cccagaccgc ccaccagatg acgaaaaaga tgaaaagttt cccgcggtac
     1801 tcaatgcgcc tgaaatggga gactaaattg cccatacgtt cctctcgagt atggttgacg
     1861 tttgaggaaa gatactactc cctaacgagg gaaatccagc gggttacgat gatcaaaaaa
     1921 attacggttg aaattctcct tcaaccgtat taactacaca tatggacttc ctcactcacg
     1981 gcctcaccgg cgctgccctg gcgcggtttt tctatggcaa agtccggccc ctcgtggcca
     2041 tcattacttt ggtcatcggc gccacgatgc ccgattacga tagtgtcgtg cgcttctgga
     2101 gtatgaccga ttttctggaa ttccatcgcg gcatatccca ctctctcgtg ggtgtgacgg
     2161 tcgccgccat cattctcgcc tcgattatga aagcaatctt ccgcgacgcc gggtatctga
     2221 aacttttcgt tttagccctc accggcggcc tctctcacat ttttctggat atgatcaact
     2281 cttacggcac gcagttgttt atgcctttca cccgctaccg ttatgcttgg gacttggtga
     2341 tgatcatcga ccccttcatc accgtaagtc tcattctcgc tctcgcgcta tcggtgattt
     2401 tcaaacgccg ccgtgtgctc ttctgcggca ttgcgattgc cttaatacta acctacgtcg
     2461 gcctgcgggc ggtctgccac gcgatagcgg tggggacggt ggagaagttc gctttacccg
     2521 gcgaaaaggt cgtggcctcc ggcgcttatc cggaaatctt gacacctttt gagtggc
//
LOCUS       DBNGZA010000432         3166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1360839, whole genome shotgun sequence.
ACCESSION   DBNGZA010000432 DBNGZA010000000
VERSION     DBNGZA010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1360839"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC16FU_09335"
     CDS             <1..547
                     /locus_tag="AC16FU_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR2994610.1"
                     /translation="WFKDEGDVILLLGENREELGGSEYLKTIHGTIGGEVPALDLDFE
                     KRVQAVALESIQSGWVKSAHDCSDGGLAVALAECCITNPQKTVGAEVDLEDKIRPDAL
                     LFGETQSRIILSASSQDASRILELAKTQKVPCRVIGRVVGSRLRMDRRLDSTRKWISE
                     DCGRLAEAFQTSLGQVLERFD"
     gene            673..1071
                     /locus_tag="AC16FU_09340"
     CDS             673..1071
                     /locus_tag="AC16FU_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR2994611.1"
                     /translation="MRKQKSLRSLAVLGSAVFFVVLTTWAISCSKKKSTNPGGGAIVF
                     QHNLNVTIRDNSFSPQYDSISVGQTITWTNAGSVIHNVTSAGGFTSSPPDLSPGASYP
                     STGGLVFNTPGDYPYQCTHHAGMTGTIRVK"
     gene            1185..1568
                     /locus_tag="AC16FU_09345"
     CDS             1185..1568
                     /locus_tag="AC16FU_09345"
                     /inference="COORDINATES: protein motif:HMM:NF021486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HIR2994612.1"
                     /translation="MAVFQKHFTLEEARGLLPQLKKIFGEIKAIRAGLETAEGDINQF
                     IEKTSGNGGSGHSPAFLQASTRIRSLLSEVAALGVQIKDINRGLVDFPVIIDGEEVLL
                     CWEYGEEDIGFWHDLESGYAGRQPL"
     gene            1664..1816
                     /locus_tag="AC16FU_09350"
     CDS             1664..1816
                     /locus_tag="AC16FU_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994613.1"
                     /translation="MELPQTLKFLNLGFWVIHIFAITIIFTLGYAMGKKKARRLSTDS
                     QRIKTD"
     gene            1891..>3166
                     /gene="hutU"
                     /locus_tag="AC16FU_09355"
     CDS             1891..>3166
                     /gene="hutU"
                     /locus_tag="AC16FU_09355"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR2994614.1"
                     /translation="MPLVAERKIKEKSDFAYKPIRAARGKKIACKGWQQEAALRMLQN
                     NLDEEVGENPRELVVYGGRGKAARNWECYFAIVRALKNLKNDETLLVQSGKPVGIART
                     FEHAPRVLIANSLLVPKWATAEVFRDLEALGLIMFGQMTAGSWIYIGTQGILQGTYET
                     FAAVANKHFGGSLAGRLVLTGGMGGMGGAQPLSATMNGAAMLCVEVDPKRIKKRLATG
                     YCDAISHDLDDALNKVLAAKKERRPLSVGLVGNCAEVLPEILRRGIIPDVVTDQTSAH
                     DELNGYVPAGLQLEEALRLRREQPEEYIKRAYISMAEHCRAMLGFQKAGAVVFDYGNN
                     LRGQAEKAGVENAFDYPGFVPAYIRPLFCEGRGPFRWVALSGDPSDIARTDAAVAALF
                     PENAGLRRWLDLAARKVKFQGLPARICWLGYGE"
BASE COUNT          720 a          799 c         1005 g          642 t
ORIGIN      
        1 gtggttcaag gacgaaggcg atgtcattct cctattgggt gagaacaggg aagagttagg
       61 cgggtcggaa tacctgaaga cgattcacgg taccattggg ggcgaagtcc ccgcgcttga
      121 ccttgatttt gaaaagcggg tgcaggcggt ggctttggag tcaattcaat ccggctgggt
      181 gaagtcggcg cacgactgct ccgacggcgg cttagcggtg gccctggcgg agtgctgtat
      241 tacgaacccg cagaagacag tgggggcgga ggttgacctt gaggataaaa tccgtcctga
      301 tgcgcttctg tttggagaga cacagtcacg gattatcctc agcgcttcgt ctcaggatgc
      361 ctctcgaatc cttgaacttg ctaagaccca aaaggttccc tgccgggtta tcggccgagt
      421 ggtcggaagc agattgcgga tggatcggag actcgactct acgagaaagt ggataagcga
      481 ggactgcggg cggctggccg aagccttcca gacctctctg gggcaggtac tggagcggtt
      541 cgactagctc accacaagcg gttaaagtag tcgggaacaa aatcaaccgc atactggtta
      601 ctactattta gtactatcgg aacgcgcagg aaagcgtatt ctgttcaaaa ggagaagaaa
      661 ggagaagaag gtatgcggaa acaaaaatct ctcagaagtc tggctgtcct cggtagtgcg
      721 gtgttcttcg tcgttcttac cacctgggcg atttcgtgca gcaaaaagaa gagcacgaac
      781 ccgggtggtg gggcgattgt gtttcaacac aatctgaatg tcaccataag agacaattcc
      841 ttcagtcccc aatatgacag catttccgta ggtcaaacga taacctggac aaacgccgga
      901 tcggtcatcc ataatgtaac ctcggctggt ggattcactt cctccccccc cgatctttca
      961 ccaggcgcat cgtatcccag tacaggtggt cttgtattta atacgcccgg ggactaccca
     1021 taccagtgta cccaccacgc tggtatgacc ggtacgatac gggtaaagtg atagattcga
     1081 gacgggctct aaaaaaatca ggcacaatgt cagtttgacc ccgcccagcg gggtcttttt
     1141 ttgcccttga caccgggcgg ggattgcgtt attagcgggt cgggatggcg gtgtttcaaa
     1201 agcattttac gctggaagaa gcccggggcc tcctgccgca actgaagaaa atcttcggag
     1261 agatcaaggc gatccgcgcc gggctggaga cggcggaggg ggatatcaat caattcatcg
     1321 aaaaaacctc cggcaacggc ggcagcggcc attcgccggc ttttttgcaa gcaagtacgc
     1381 ggattcgctc acttttgagc gaagtggcag cgctgggggt gcagatcaaa gacatcaacc
     1441 gggggctcgt cgatttcccc gtgataatcg atggagaaga agtcctcctc tgctgggagt
     1501 acggggagga ggatatcggg ttctggcatg atttagagag cgggtatgcg ggaaggcaac
     1561 ccctatgaaa gagaaaaata gtagccagta gccaggggcc agtagccaga aacctaattt
     1621 ctaaatccta tatccgaact ttgggctacg tgagggagga gagatggaac taccgcagac
     1681 gctgaaattt ctcaatctgg ggttctgggt cattcacatt tttgccatta ccatcatctt
     1741 taccctcggg tatgcaatgg ggaagaagaa agcgcggcga ctgtcaacgg attcgcaacg
     1801 gattaaaacg gattaaagga gtggattctt ctccgtaaaa cgttgaggcg cgttgacggc
     1861 cagcgaatat ccccgtagtt tacaattgca atgccgcttg tagccgaaag gaaaataaaa
     1921 gaaaagtcgg actttgcgta taagcccatc cgggcggcgc ggggaaagaa aattgcctgc
     1981 aaggggtggc agcaggaagc ggccctgcgg atgctccaga acaatctcga cgaggaagtc
     2041 ggggaaaatc cgcgggagtt ggtcgtctac ggcgggcggg gaaaggcggc gcggaactgg
     2101 gagtgttatt tcgccatcgt ccgggcgctc aagaacctca aaaatgacga aacgcttctc
     2161 gttcaatcag gcaagccggt gggcattgcg agaacgttcg aacacgcgcc gcgggttctg
     2221 atcgccaatt cgcttttggt gccgaagtgg gcaaccgccg aggtgttccg cgacctggaa
     2281 gcgctggggc tcatcatgtt cggccagatg accgcggggt cgtggattta catcgggacg
     2341 caggggattc tgcaggggac gtacgaaacc tttgcggcgg tggccaataa gcacttcggg
     2401 ggcagtctgg cgggacgctt ggtcctgacc ggcgggatgg gcggcatggg gggcgcgcag
     2461 ccgttatccg cgacgatgaa cggcgcggca atgctctgcg tggaagtcga tccgaagcga
     2521 atcaaaaagc gtctggccac cggttactgc gatgcgatca gtcacgatct cgacgatgcg
     2581 ctcaataaag ttctggctgc taaaaaagag cggcggccgc tctccgtagg attggtgggc
     2641 aactgcgcag aggtcctgcc cgaaattttg cggcgcggga ttatccccga cgtggtcacc
     2701 gaccagactt cggcccacga tgaactgaac ggctacgttc ccgccggttt gcagttggaa
     2761 gaggcgctgc gcctgcggcg cgagcagccg gaggagtaca tcaagcgggc gtacatttcg
     2821 atggccgagc actgccgggc gatgttggga ttccagaaag cgggcgcggt ggtctttgac
     2881 tacggcaaca acctgcgcgg gcaggccgag aaggccgggg tcgaaaatgc cttcgattac
     2941 cccggctttg tgccggcgta catccggcct ttattctgcg agggacgggg gccgttccgc
     3001 tgggtggcgc tctccggcga tccctccgac atcgcccgca ccgacgcggc ggtcgcggcg
     3061 ctcttcccgg agaacgcggg gttgcgccgc tggctcgacc tcgccgctcg caaggtgaag
     3121 ttccaggggc tgccggcgcg gatctgctgg ctcggctacg gggagc
//
LOCUS       DBNGZA010000433         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2420710, whole genome shotgun sequence.
ACCESSION   DBNGZA010000433 DBNGZA010000000
VERSION     DBNGZA010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2420710"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1610
                     /locus_tag="AC16FU_09360"
     CDS             9..1610
                     /locus_tag="AC16FU_09360"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR2994615.1"
                     /translation="MDNAILSWVVFLPLAGVPLLAFINRESKWALKLTALAVSTITFL
                     VSLFLFFRFDPQNPGFQFSIDVPWIPSFGIHYHLGLDGISLFLVLLTTFLTPIAIWSS
                     WTAITEKLKGYLISILILESAMSGVFLALDLFLFFVFWEAILIPMYFLIGIWGGPRRI
                     YATIKFVLFTMTGSLLMFVAIIVLFFLYHSHTGQYSFNLLDYYKLPIPLGAQTWLFLA
                     FALAFAIKVPMFPFHTWLPNAHVEAPTAGSVILAGILLKMGTYGFLRFCWPLFPNAFE
                     QFLPMMIVLSLIGIIYGAWMSMVQKDIKSLIAYSSVSHLGFVMLGLAVLNLQGLQGSL
                     LQMLNHGISTGALFLIVGMIYERRHTRLIADYGGITKVVPVFATFFLIVALSSIGLPG
                     TNGFVGEFLILLGAFKFNWIYAALGATGVIFAAVYMLWMYQRVMFGPVTNPANSALTD
                     LNRRERWTLIPLVVLIFWIGLYPKPFLSRMEASLSQFVTQVQERREYLSQYRNGKIVS
                     PVPQGLLTSTPQHPDTSTRSQVNRP"
     gene            1607..>1974
                     /locus_tag="AC16FU_09365"
     CDS             1607..>1974
                     /locus_tag="AC16FU_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994616.1"
                     /translation="MNIIPPDLVFSGLVPEIVVVGFATLLMVVGPFFKQRGQDVVFWL
                     TLIGLALAAFTSILLWNQTISAMGGMAYSDNFATFFRLVFLLAAFVVVLFSKAYLKWE
                     NLPAAEYYSLLLFSTFGMML"
BASE COUNT          338 a          574 c          501 g          561 t
ORIGIN      
        1 gaaaaaaaat ggataatgca attttaagct gggttgtctt cctgccgctg gccggggtgc
       61 ccttgctggc cttcatcaat cgggagagca aatgggcgct caaactcacc gcgctggcag
      121 tttcgaccat taccttcctg gtctcgcttt tccttttctt ccgattcgat ccacagaatc
      181 ccggatttca gttttcgatt gacgtgccct ggattccgtc cttcggcatc cactaccacc
      241 tgggactcga tggcatcagc ctttttttag tcctcctcac tacatttttg acgcccattg
      301 ccatctggtc gtcctggacg gccatcacgg aaaaactcaa ggggtacttg atttcaatac
      361 tcatcctgga atcggcgatg agcggcgtct ttctggcgct ggatttattc ttattcttcg
      421 tcttctggga agcgattctc attccaatgt actttttgat cggcatctgg ggcgggccgc
      481 gccggattta cgccaccatc aagttcgtgc tcttcactat gaccgggtcg cttttgatgt
      541 tcgtggccat catcgtcctg ttcttcctct accacagcca caccggccag tattcgttca
      601 acctgcttga ctactacaaa ttgccgattc ctctgggcgc gcagacctgg ctttttctgg
      661 cgtttgcgct ggcctttgct atcaaagtgc cgatgttccc cttccacacc tggctgccga
      721 atgcgcacgt ggaggcgccc acggcgggga gcgtcatctt agcgggcatt ctgctcaaga
      781 tggggacgta cggattcttg cgcttctgct ggcccctttt cccgaatgcg ttcgagcagt
      841 tcttgccgat gatgatcgtc ctttctctca tcgggatcat ctatggtgcg tggatgtcaa
      901 tggtgcagaa agacatcaag agtctgattg cctactcgtc ggtgagtcat ctggggttcg
      961 tgatgctggg tttggccgtg ctcaatcttc aaggtctcca ggggagtctc ttgcagatgc
     1021 tcaaccacgg catttccacc ggggcgttgt tcttaatcgt cgggatgatt tacgagcgca
     1081 gacatacccg gctgatcgcc gactacggcg gcattaccaa ggttgttccg gttttcgcta
     1141 cctttttcct gatcgttgct ctgtcatcca tcggcctccc cggcaccaac ggctttgtgg
     1201 gtgagtttct catccttctg ggcgctttta aattcaactg gatttacgcc gctctgggag
     1261 cgaccggcgt catcttcgcg gccgtctaca tgctctggat gtaccagcgg gtgatgttcg
     1321 gcccggtgac caatccggcc aactcggcat taacggacct caaccggcgt gagcggtgga
     1381 ccttgatccc tttggtcgtt ctcatcttct ggatcggcct ctaccccaag ccgtttctct
     1441 cccggatgga ggcgtctctc agccagttcg tcacgcaggt gcaggagcgg cgggagtatc
     1501 tcagtcaata ccgtaatggt aaaatcgtat caccagtgcc ccaaggtctt ttaacttcga
     1561 cacctcaaca ccccgacacc tcgacacgct ctcaagtgaa ccgcccatga atatcattcc
     1621 ccccgatctc gttttcagcg gcctggtgcc ggaaatcgtc gtggtgggtt tcgcaaccct
     1681 cttgatggtc gtggggccgt ttttcaagca gcgcggccag gacgtagtct tctggcttac
     1741 gcttatcggt ctggctttgg ccgcgttcac cagcattttg ctctggaatc agaccatttc
     1801 cgccatgggc gggatggcct actccgataa ctttgccacc ttctttcggc tggtgttcct
     1861 tctggcggcg ttcgtcgtcg ttcttttctc aaaagcgtac ctcaaatggg agaatctgcc
     1921 ggcggcggaa tactattcgc ttttgctctt ttcgaccttc ggcatgatgc tcat
//
LOCUS       DBNGZA010000434         2793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_617220, whole genome shotgun sequence.
ACCESSION   DBNGZA010000434 DBNGZA010000000
VERSION     DBNGZA010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_617220"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC16FU_09370"
     CDS             complement(<1..861)
                     /locus_tag="AC16FU_09370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR2994617.1"
                     /translation="MAEVILKPKETRRLQGGHLWIFSNEIQEVRDVGENGQLVAVRGT
                     GGELLGRGYYNRHSLIAVRLLTRRQDEEIDSAFFRSRIARAIEYRRRLYPDSISGRLI
                     YSESDLLPGLIVDRYESVLVLQVTTLGLERLLPICLPVLEELLRPTGIFLRNDSSLRL
                     LEGLLQETKVLSGDVPDEMEISQHGLKFLVDVKSGQKTGFFFDQRENRERMRVKVALR
                     GKRVLDCFCYTGAWALVAAQAGAQSVIGLDESNAAIVLAQKNAALNGLADRVTFRKAE
                     VFSELRRLVSA"
     gene            complement(926..1915)
                     /locus_tag="AC16FU_09375"
     CDS             complement(926..1915)
                     /locus_tag="AC16FU_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR2994618.1"
                     /translation="MPKEIWIFAEQKYGNLSRTAFELLTVAQKLAEPKSASVAAVLFG
                     HQSEGLTAELFGHGAQKVYLADAPGLEKFVDESYAAVLADLIQKHQPEIVLGAATFSG
                     KALFPRLAALLQAGIITDAIAISADTQPLAVTRPAYGGNVFQELTAQGAGSLLLTCRP
                     KAFAEAPAAPGRTGEIVRHPVPEELLTSPTTVREAVKAEGQKVSLTEADVIVSGGRGL
                     REPANFKLIADLAAALGGAVGASRAVVDAGWIPYAHQVGQTGKTVNPKLYIACGVSGA
                     IQHRVGMQSARVIVAINRDKDAPIFDIATYGIVGDLFEIVPALIKKFKEKLGR"
     gene            complement(1921..2718)
                     /gene="larC2"
                     /locus_tag="AC16FU_09380"
     CDS             complement(1921..2718)
                     /gene="larC2"
                     /locus_tag="AC16FU_09380"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC2"
                     /protein_id="HIR2994619.1"
                     /translation="MDTIVDVVGSVAGFETLGIEKIYSSPLPLGRGMIASEHGPLPLP
                     APATAELVRNYPVRPVDIEGELVTPTGAAILTTLAEFTAPPPYRLLTVGYGAGSRNLA
                     NIPNVLRLFIGETVPAPAVDTVTQIETNLDNTSPEVLGYLAERLFSAGALDVYFVPVT
                     MKKSRPGVLLGLLANPEDREKFAAILFEETGTIGLRYWECQRRKLPRSEEVVQTKYGA
                     VRLKISELDGQKRAAPEYADCARLAEQHHVPYRLIVAEALKAFGLKK"
BASE COUNT          564 a          833 c          820 g          576 t
ORIGIN      
        1 ggcgcttacc agacggcgca attcgctgaa gacttcggct ttccggaaag taacacggtc
       61 agccaggcca tttaacgccg cgttcttctg ggccagcaca atggcggcat tcgattcatc
      121 cagtccgata accgactgcg cgcccgcttg agcggccacc agtgcccagg ccccggtgta
      181 gcagaagcag tcgagtaccc gtttacccct cagggccact ttcacccgca ttctttcgcg
      241 attctcgcgc tgatcgaaga aaaatccggt cttctgaccc gattttacgt caacgagaaa
      301 cttcaagccg tgctggctga tttccatttc gtccggcacg tcaccggaga gaaccttcgt
      361 ttcctgcagc aagccttcta acaggcgcaa ggaagaatcg ttgcgaagga aaataccggt
      421 tggacggagc aattcttcca gcaccggcag gcagatcggc aaaagccgct ccagtcccag
      481 tgtggtcact tgcagtacga gaaccgattc ataccggtct acgatcaggc cggggaggag
      541 atcgctttca ctgtaaatca ggcgtccgga aattgagtcg gggtacaatc tccttcgata
      601 ctcaattgcc ctggcgatgc gcgagcggaa aaaggcactg tcaatttcct catcctgcct
      661 gcgcgtcaac aaacgcacgg cgatcaaact gtgccggttg tagtacccgc gccccagtag
      721 ttcaccgccg gtgccgcgca ccgccacgag ttggccgttc tctccaacat ctcgcacctc
      781 ttgaatttcg ttggagaaaa tccagagatg gccgccctgc aggcggcgcg tttctttcgg
      841 tttgaggatg acttctgcca ttggggagga ggaagagggc tgatctgata gtaaggccgg
      901 aggggagaag gggacgtgcg taccgctacc gccccaattt ctctttgaac ttcttgatca
      961 gagcggggac gatttcaaac agatccccca cgattccgta agtggcgatg tcaaaaatcg
     1021 gcgcatcctt gtcgcggttg atggcgacga tgacccgggc cgactgcatc ccgacccggt
     1081 gctggatggc gcccgaaacg ccgcaggcga tgtagagttt ggggttgacc gttttgccgg
     1141 tctggcccac ctggtgggcg tagggaatcc agccggcatc caccaccgcg cgagaagcac
     1201 cgaccgcgcc gcccagggcg gcggccagat cggcgatcaa tttgaaattc gccggctcgc
     1261 gcaagccccg gccgccggag acgataacgt cggcctcggt caaactgacc ttctggcctt
     1321 cggctttgac cgcttcgcgt accgtcgtcg gcgaggtcaa tagttcttcc ggaacagggt
     1381 gccgaacgat ttcacccgtg cggccgggtg cggcgggtgc ttcggcaaaa gctttgggcc
     1441 ggcaggttaa gaggagtgag cctgcgccct gagccgtcag ttcttggaaa acattgccgc
     1501 cgtaagcggg gcgcgtcacc gccagtggct gagtgtcggc ggaaatggcg atggcgtcgg
     1561 taattatgcc cgcctgcaaa agcgccgcca ggcgggggaa aagcgcttta cccgaaaagg
     1621 tggccgctcc cagcacgatc tccggctggt gcttttgaat caggtcggcc agcaccgccg
     1681 cgtagctttc atccacgaat ttttcgaggc cgggggcgtc ggccagatag accttctgtg
     1741 ctccatggcc gaacagctcc gcagttaacc cttcactttg gtgaccgaac aaaacagccg
     1801 cgaccgaagc acttttcggc tcggctaatt tttgggcgac ggtcaatagt tcgaaagcgg
     1861 tgcgagagag attcccatat ttctgctccg cgaaaatcca gatttctttg ggcatagcct
     1921 ctactttttc aggccaaagg ctttcaaggc ctcagcgacg atcagccggt acggcacatg
     1981 gtgctgttcg gctaagcgcg cgcaatcggc atactcgggc gcggcgcgct tctgcccgtc
     2041 caattcactt attttcaacc gtaccgctcc gtatttggtc tggaccacct cctcgctgcg
     2101 cggcaacttc cgcctttgac attcccaata gcgcaggccg atggttccgg tttcttcgaa
     2161 gaggatggcg gcgaacttct cccgatcttc cgggttagcc aggaggccga gcaagacgcc
     2221 ggggcggctc tttttcatcg tcaccggaac gaagtatacg tcaagtgccc ccgcagaaaa
     2281 aagccgttcg gccagatagc ccaggacttc cggactggtg ttgtcgagat tggtctcgat
     2341 ctgcgtcacc gtgtcgactg cgggtgccgg taccgtctcc ccgatgaaca accgcaggac
     2401 gttgggaata ttggcgagat tccgggagcc agcgccgtag ccgacggtca aaagccgata
     2461 cggcggtggg gccgtgaact ctgccaaagt ggtgagaatg gccgctccgg tgggagtgac
     2521 cagttcccct tcaatgtcca ccgggcgcac ggggtaattc cgtaccagtt cggctgtggc
     2581 cggggccggt aagggcagag gcccgtgctc tgaggcaatc attccccgcc ccagaggcaa
     2641 aggggaagag tagatttttt ctattcccaa cgtttcgaag ccggcgacgc tgccgacaac
     2701 gtccacgatc gtgtccaccg cacctaacta gtggaagtgt ctttcctcaa acgaacttcc
     2761 aagcacgcgg gcttcggcct ctgcctagcg gtg
//
LOCUS       DBNGZA010000435         7764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2631438, whole genome shotgun sequence.
ACCESSION   DBNGZA010000435 DBNGZA010000000
VERSION     DBNGZA010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2631438"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..667)
                     /locus_tag="AC16FU_09385"
     CDS             complement(11..667)
                     /locus_tag="AC16FU_09385"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994620.1"
                     /translation="MKEDRLVTSVFIAFEYLQRNYQRLAIGIGAVLVVILAVFLYNRG
                     QKQKALEAEAACGQAQLRVTAGDLQGGILDLRRVIELYGGTAAGQKATYFLANAYLQA
                     RDYRQAEQLFRQFVAGGYKERLLVGSAHMGLGVCLEETGKTAEAAGEYLKAVEISGKD
                     YTTPEYALAAIRALAKTGQKEKALDLFNRLLNEFPRNFTDHNRARELLARYGIFDYGS
                     "
     gene            complement(820..2250)
                     /locus_tag="AC16FU_09390"
     CDS             complement(820..2250)
                     /locus_tag="AC16FU_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR2994621.1"
                     /translation="MKKSFAISVVLGSLFLSTVASAQFITNYSFLGGGPRARGMGGAS
                     IGVADDPSTATWNPAGLTLLDRVQTGESFSYLRPRVKGEPSLTAGTRSFLNSFSSNKN
                     MGVINYASFILPGKVGGRQMVGALAFRRAEELFQDIALASDSTRYKRHLEGSLNVLNL
                     AVGTKITDAFAVGAGLNVYLGGYDRTEQINFTVTDTALYHQPYGAQQIFRYNWNQQSN
                     FRGVNFELSTLYLGPKYRLGAIVRTPFQLKDKFETRRGDTLFVDNVPNFFYSRGLFTP
                     RTATELGIPLSVGVGGSYKATEALTVAVDFEARLYSQADLSVPDSILDPASPLHSQNL
                     NWSDDFQVRFGLEYLLQSGGMKFPLRLGARNEPQIFEDARLLEAAASPYSSDVNNERL
                     DKGTPIFAGRTVRKQGIVGTAGAGLQMAQIHLDLTYELGTVKPQETGSYVDPSAPKTG
                     GVRSTTFTTAQDHLSHQVTFSFTGYF"
     gene            2751..3419
                     /locus_tag="AC16FU_09395"
     CDS             2751..3419
                     /locus_tag="AC16FU_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR2994622.1"
                     /translation="MVEDDPDIRELLARYLKREEYQVLLAKDGEEGLERAGKVHPDLI
                     ILDLMLPRQDGLSVLKDLRRGEATTAIPVLILTAKGEEPDRIAGFEFGADDYVTKPFS
                     PREVAARVKALLRRVTQPGKPAEVQYLYGTLIVDTEQHKVFYEKEEVQLTAKEFSLLK
                     LFLDNRGKLLTRERILARVWGTDFSGGPRTVDVHIRRLREKIQLLEKSIATVKSYGYR
                     LEEP"
     gene            3416..5173
                     /locus_tag="AC16FU_09400"
     CDS             3416..5173
                     /locus_tag="AC16FU_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2994623.1"
                     /translation="MRFGFRSKLIFSLVVLFALSFVASTVVVEKLVRNFWIGQLKKAL
                     EASARLLAFELTGPLQTPGYERLDQAADTYGQLLALRVTVIDTTGKVWGDTQVGGDTL
                     VTLENHASRPEVAAALSGKTGSSIRFSTTVKTEMLYLAYPISDGGHVVGVVRLARPLA
                     EVNEAIARIRRTILIFFLVVFATLSIAGAIFFLRQSRPVKQLVGHFERLKAGDFSARV
                     SVTSKDEFLLLGQTVNELAERLEQSFAELKEQKQVQDTTLHTLTEGVLAADGSGRIVF
                     SNPALRTIFHLEGGFIGQPFHTVFRQPGLLAAFDGVLKEGGIQKVTIKETFPTHRVIE
                     TYFSSPRETRTVRLVAVFQDITQAYRLEQVRKDFVANASHELRTPLSVVKGYVETLED
                     GLVSEEERKAVFQTLGRNIERMGLLIGDLLHLSKLESPEFTLNIGRVTLTEVARAALS
                     SLQSKIAAKKQKLTLDLPPDLSLRADRLELEVALKNLLENAIQYTSEGSAITLRAAEQ
                     NGTVVVTVADTGIGIPSQELERIFERFYRGDKSRSREEGGTGLGLSIVKHIAEAHGGR
                     VEVESEVGKGSSFTLILPS"
     gene            5338..6573
                     /locus_tag="AC16FU_09405"
     CDS             5338..6573
                     /locus_tag="AC16FU_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994624.1"
                     /translation="MKGRAMILAVTILLCLAPVGVRAQETASDSLKEKVTETADKVAG
                     IEERLGVDESDIGNLKKLKISGYVQARYEYHDDAVSTPALGTASTASSNQGKLLNRFF
                     IRRGRLKATYQATPNALGVVYFDGSASGVTLKEAYVVVSEPHSKVTATLGQFNWLFGY
                     EISYSSSKREFPERARWSRTLFPGERDRGVKVERPFLLAGKYTLTLTGGVYNGNGIED
                     AAFGVRDPNKAKDFLARAAVGLGHLDFGFSGYWGKQFNPSDSVIPKQQPSTTDKIRYG
                     ADAQFFYELPTLGGGVLKAEGVLAKEPKNQSKPFLSRDTTRDVAGFNVVWAQNLKEKF
                     QWISRVDFYDPNRDPDQDHFITYGLGLIYFWDGNSKVKLVYEIPKSYRNSTSGTVTVN
                     GVKIDEKDNLLTLEWVYTF"
     gene            6599..7456
                     /locus_tag="AC16FU_09410"
     CDS             6599..7456
                     /locus_tag="AC16FU_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR2994625.1"
                     /translation="MRHRGFLVTAALAFGLGGAGLGLAGKSITVKGSDTMVILGQRWA
                     EIYMQKNPGSVIQVTGGGSGTGIAALINGTTDICEASRPMKDAEKTKLKERYFTLGVE
                     IPVAKDGLAVYLHETNPVEKLSLEQLKAIYTGQITNWNEVGGPDAKIILYGRENNSGT
                     YVFFKDNVLKGADFAPQTATLPGTAAVVNAVAKDKNGIGYGGAAYAKGIKFCKVSEKT
                     DGQAFEPNLATVESGQYPLSRDLYWYLRSRPTGDVKKLVDWVLSPEGQKVVSEVGYFP
                     VKSARAAEK"
     gene            7460..>7764
                     /locus_tag="AC16FU_09415"
     CDS             7460..>7764
                     /locus_tag="AC16FU_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994626.1"
                     /translation="MPATRDGAARNLHIKGGAPPKIDRRKLIERGVELVVYLSSWVSI
                     LGIVFLFIFIGKEAFPLLTDPAMKKEVQFSQLWTSIWQPVSDQPKFGLIPLFLGTLKA
                     "
BASE COUNT         1739 a         2135 c         2258 g         1632 t
ORIGIN      
        1 tgccggtcag ttaacttccg tagtcgaaaa tgccgtagcg ggccagaagt tcgcgggccc
       61 ggttgtgatc ggtgaaattc cgcgggaact cgttcaacag ccggttgaat aaatcgagtg
      121 ctttctcctt ctgccccgtc ttggccagag cgcggatggc ggccagcgcg tactccggcg
      181 tggtgtaatc tttcccggat atttccaccg ctttcaggta ctctccggct gcctcggcgg
      241 ttttgcccgt ttcctccagg cagacgccca gacccatatg tgcggagcca accagcaagc
      301 gttccttgta cccgccggcg acaaactgcc gaaagagttg ctcggcctgc ctgtagtctc
      361 tggcttgcag gtaggcgttc gccagaaagt aggtcgcctt ctggcccgcc gccgtaccgc
      421 catacagctc tatcacccgg cggaggtcga gaatgccgcc ctggaggtcg ccggcggtga
      481 cccgcagctg ggcctgaccg caggcggctt cggcctccag cgctttttgc ttctgtcccc
      541 ggttgtaaag gaagacggcc aagatcacga cgagcacggc gccaatcccg atggccagcc
      601 gctggtagtt ccgctggagg tattcaaaag cgatgaagac cgacgtgacc aatcggtcct
      661 ctttcatttg gtgcttggta atccgctttt cgggactcat ggttgccgct taaaaccaga
      721 tgacatctgc ttggtcggaa gagtcaaaac cggatgaacg cacaaccaga caaaactgac
      781 cggaacgccg ttggcggggc gccacgggaa atccggcatt taaaaatacc cggtgaaact
      841 gaaggtgacc tggtgcgaca ggtgatcctg tgccgtagtg aaggtcgtgc tccgcactcc
      901 tcccgttttc ggggcgctgg gatcgacata cgagccggtc tcctggggtt ttacggtacc
      961 gagttcatag gtcaggtcga ggtggatctg ggccatttgc agaccggctc cggccgtgcc
     1021 tacgatcccc tgtttccgca cggtgcggcc ggcgaaaatc ggggtacctt tgtccaggcg
     1081 ctcgttattg acatcagaag agtagggaga tgcggccgct tccaagagac gcgcatcttc
     1141 aaaaatctgc ggctcattcc gcgcgccgag ccgcaggggg aacttcattc ctccactctg
     1201 caaaagatac tcaaggccga aacgcacctg aaagtcgtcg gaccaattca ggttttggct
     1261 gtgcagcgga gacgccggat ctaaaatcga gtcgggtacc gagaggtcgg cctgggagta
     1321 aaggcgcgcc tcgaaatcga ccgcgacggt caaggcctcg gtggctttgt aggagccgcc
     1381 cacgccgacc gagaggggta tgcctaattc cgttgccgtg cggggggtga agaggccgcg
     1441 ggagtaaaag aaattgggga cgttatcgac gaagagcgtg tccccacgcc gggtttcgaa
     1501 cttatccttc aactggaacg gagtgcggac gatggccccc agccggtact tgggccccag
     1561 gtagagggtc gagagttcaa agttcacgcc gcggaagtta gactgctggt tccagttgta
     1621 ccggaaaatc tgctgggctc cgtaaggttg atggtagagc gcggtgtcgg tgacggtgaa
     1681 gttgatctgt tcggtacggt catagccgcc gaggtaaacg tttaacccgg cacccaccgc
     1741 aaaagcatcg gtgattttgg tccccaccgc cagattcaag acgttcagac tcccttcgag
     1801 gtggcgttta tatctggtgc tgtcgctggc cagcgcaatg tcctgaaaca gttcttcggc
     1861 gcgccggaaa gccagagcgc ccaccatttg ccggccgccc actttgccgg ggagaatgaa
     1921 actggcgtag ttgattacgc ccatattttt gtttgaagag aacgaattca aaaacgaacg
     1981 cgtgcccgcc gtcagggacg gctcgccttt gacccgcgga cgcaggtagg agaacgactc
     2041 gccggtctgc acccgatcca aaagggtgag gccggccggg ttccaggtgg cggtcgaggg
     2101 atcgtcggcc acgccgatgg aggccccgcc cataccgcgg gcgcgcggac cgccgccgag
     2161 gaaactatag ttcgttatga actgggccga agcgaccgta ctcaaaaaca gactgcccag
     2221 caccacactg attgcgaacg atttcttcat tgaattaacc ctccactcac tcaaaagagc
     2281 ggggttgaaa ccccgcctac gcgttgccgt tcttcccgca aaaacactcc attctaccct
     2341 acccaccgcg atttgtcaac tcgatttttt gcctttttcc tttggtgttc atctctaatc
     2401 ccttatccct aatccctgct tttgaagtac ccccgggccg attcgaacgg ccggccaacg
     2461 gtttaggaaa tctaaataag aagaccgggc cctcccaccc cgacttgttg agttacatta
     2521 ggttacgcca taagtaaata agatccaaaa agttacgcga tcggttaatc tgtccacatg
     2581 atggggtaga gtgcgatact ttatcgtatg gtgcgatcta tttggggcgg cgattcgtag
     2641 caaaagtcgt agcagtctaa catgttttca cggcagaaat caactatctc ctcttgtaaa
     2701 aaagtaattt ctacccttcc tttaggaccg tgaaggccaa agtcattcta gtggtcgaag
     2761 atgacccgga tattcgcgag ttactcgcac gctacctgaa gagggaagag taccaggtac
     2821 ttttggcgaa agacggtgag gagggtctgg aacgagcagg aaaggttcat cccgatttga
     2881 ttattttaga cctgatgctt ccgcgtcagg acggcctttc ggtactcaaa gacctgcgtc
     2941 gcggagaagc taccactgcc ataccggttt tgattttgac cgccaagggc gaagaacccg
     3001 accgcattgc cgggtttgag tttggagcgg atgactatgt aaccaagccc ttttccccgc
     3061 gtgaagtggc cgcgcgggtg aaggccttgc tgcgccgggt cactcaaccg ggaaaacccg
     3121 ccgaagtgca gtacctgtac ggtactctga ttgtagacac cgagcagcac aaagtattct
     3181 atgaaaaaga ggaagtacaa ctgacggcta aagagttcag cctgttgaaa ctctttttgg
     3241 ataaccgcgg caagctcttg acgcgcgaac gcatcctcgc ccgcgtgtgg ggaacagact
     3301 tttccggtgg tccccgcact gtggacgtgc acatccgccg cttgcgtgaa aaaatccagc
     3361 tcttggaaaa atcgatcgca acggtgaaat cgtacggcta ccgcctggag gagccgtgag
     3421 attcgggttc cggtccaagc tcatcttttc cttagtagtg ctttttgcgc tttccttcgt
     3481 tgcgagtacc gttgtcgtgg aaaaactggt gcgtaacttt tggattggtc aattgaaaaa
     3541 agcgctggag gccagtgccc ggctgctcgc gttcgaactt acgggaccgc tccagactcc
     3601 cggatatgaa cggttagatc aagccgccga cacctacggc cagctcttag ccctgcgggt
     3661 caccgtgatt gacaccacgg gaaaggtctg gggtgatacc caggtaggcg gggatactct
     3721 tgtgacttta gaaaatcatg ccagccgtcc tgaagtcgct gcggcccttt cgggcaaaac
     3781 ggggtcttct atccgttttt ccacgacagt taaaaccgaa atgctctatc ttgcctatcc
     3841 aatctcagac ggcggccacg tagtgggcgt ggtgcgtctc gcccggccgc tggcggaggt
     3901 gaatgaggcg atagccagaa tccgacgcac gattctgata ttctttttag tggtctttgc
     3961 gacactttcc attgccggag caattttctt tttgcgtcag tcccggccgg tgaagcagtt
     4021 ggtcggtcat tttgagcggc tgaaggcggg cgacttttca gcccgggtca gcgtcacaag
     4081 caaggacgaa ttcctgctcc tcggtcaaac cgtaaacgaa ttggcggagc ggctggagca
     4141 aagctttgcg gaactcaaag agcaaaagca ggtgcaggat accacactgc acacactgac
     4201 ggaaggagtg ctggcggcgg atggcagcgg ccgaatcgtt ttcagcaacc cggcgctgcg
     4261 tacgatcttt cacctcgaag gggggttcat tggccagccg ttccatacgg tttttcgcca
     4321 gcccggtctt ttggctgcat ttgacggagt actgaaagag gggggtatcc agaaagttac
     4381 tatcaaagaa acattcccca cgcatcgagt aatcgaaacc tatttctcga gcccccggga
     4441 gaccagaacc gtccggctgg tggccgtttt ccaggacatc acccaggcct atcggctgga
     4501 gcaggtgcgt aaagactttg tcgcaaacgc ctcgcacgaa ctccgtactc cgctctcggt
     4561 ggtcaaaggg tacgtcgaaa cgctggagga cggcctcgtc tcggaagagg agcgaaaagc
     4621 ggtattccaa accctcggca gaaatattga acggatgggt ctcctgattg gcgacctcct
     4681 gcatctctcc aaattggagt cgccggagtt taccctgaac atcgggcggg tgacattgac
     4741 cgaagttgcc cgggccgcgc tttcgagtct gcaatcgaaa atcgccgcaa aaaaacaaaa
     4801 gttgaccttg gacctgccgc ccgacctttc cctccgggcc gaccggttgg agctggaggt
     4861 agcccttaag aaccttctgg agaatgcgat tcagtacacc tcagagggga gcgcgattac
     4921 tctccgggcg gctgaacaga acggaacggt ggtggtcacc gtcgccgata cgggcatcgg
     4981 cattccatcg caagagttgg aacggatctt tgagcgtttt taccgggggg ataaatcccg
     5041 ttcccgcgaa gaggggggca ccggcctcgg tctctcgatt gttaagcaca ttgccgaagc
     5101 ccacggcggg cgggtggaag tcgaaagcga agtcggcaaa ggctcaagtt ttactctgat
     5161 tcttccttct taacgtttct cttcaagact aaaaccttcg ttcatcaggc ggttgtctcc
     5221 gaaaccgctg gcaactgtta acaccgattt aacattcccg atatttgtcg gaaataatga
     5281 aagcgtatct cctcctgaac gaatggttcg actagttcat caaaaaagga gggatgcgtg
     5341 aaagggagag cgatgatttt ggcagtcaca atcctcttgt gcttggctcc agtcggggtc
     5401 agggcgcagg agacggccag cgacagtcta aaggagaagg taaccgagac ggcggacaag
     5461 gtggctggca tcgaggaacg gcttggtgtc gacgagtcgg acatcggcaa cttgaagaag
     5521 ctgaaaattt ccggctacgt gcaggcccgc tacgagtacc acgacgacgc cgtgagcacg
     5581 ccggctctgg ggaccgcctc aactgccagc agcaatcagg ggaaactgtt aaaccggttc
     5641 ttcattcgcc ggggccggct caaggcgacc taccaggcga ctcccaacgc gctgggcgtg
     5701 gtctacttcg acggctccgc ctccggggtt acgctcaaag aggcctacgt ggtcgtaagc
     5761 gaaccccatt ccaaagttac cgccaccctg gggcagttca actggctctt cgggtacgag
     5821 atttcctact cctcatccaa gcgggagttt ccggagcgcg cccgctggtc ccgcacactc
     5881 tttccgggag agagggaccg gggcgtgaaa gtggagcggc cgtttctttt ggccggaaag
     5941 tacaccctga cccttactgg cggagtctac aacggcaacg ggattgaaga cgcagccttc
     6001 ggggtacggg atcccaataa ggcaaaagat tttctcgccc gagccgccgt cgggctgggg
     6061 catctggact tcggtttttc cggctactgg gggaaacagt tcaatcccag cgattcggtc
     6121 atccccaaac agcagccttc gaccaccgac aaaatccgct atggcgccga cgcgcagttc
     6181 ttctacgaac tgcccacgct cggcggcgga gtgttgaagg ccgagggagt tctggccaaa
     6241 gagccgaaga accagtccaa accgttcctc tcgcgtgaca ccacccgcga cgtggccggt
     6301 ttcaacgtcg tctgggcgca gaatttgaaa gagaagtttc agtggatttc acgggtcgat
     6361 ttctatgacc ccaaccgcga ccccgaccag gatcatttca tcacgtacgg cctgggcttg
     6421 atttacttct gggacggcaa cagcaaagtg aaactggtgt acgagattcc caagtcgtac
     6481 cggaacagta cgtcgggcac cgtgaccgtc aacggagtga aaatagacga gaaggacaac
     6541 ctgctgacgc tggaatgggt ctacactttc taatcgcaac caaaagaagg agggaaagat
     6601 gagacacaga ggttttctgg taacggcagc cctcgcattc ggcctcggcg gcgcagggct
     6661 aggtctggcg ggaaagtcga tcaccgtcaa gggctcggat acgatggtga tattgggcca
     6721 gcgctgggcc gagatttaca tgcagaaaaa tcccggttcg gtgattcagg tcaccggcgg
     6781 gggcagcggc accggcatcg cggcactgat caacggcacg accgacatct gtgaagcctc
     6841 ccgcccgatg aaagacgcgg aaaagacgaa gttgaaagag cgctacttca ccttaggggt
     6901 tgaaatcccg gtagccaaag acgggctggc cgtctacctg cacgaaacca acccggtcga
     6961 aaagctttcg ctggagcagt tgaaagcaat ttacaccggg caaatcacca actggaatga
     7021 ggtgggcggg ccggatgcca aaatcatcct gtacggacgg gagaacaact ccggcaccta
     7081 cgttttcttc aaggacaacg ttttgaaagg tgccgatttc gctccccaga ccgccacttt
     7141 gcccggcacg gcggcggtgg tgaacgcagt cgccaaagat aaaaacggca tcgggtacgg
     7201 gggagccgcg tatgccaagg ggatcaaatt ctgcaaggta tcggaaaaaa ccgacgggca
     7261 ggcgttcgag ccgaatctgg ccacggtgga aagcggccag tatcctcttt cgcgcgacct
     7321 gtactggtat ctgcgcagcc ggccaaccgg cgacgtcaag aaattggtcg attgggtgct
     7381 gtccccggaa gggcagaaag tggtgagcga ggtggggtat ttcccggtca aatccgcccg
     7441 ggcggctgaa aagtaaacaa tgcctgcaac ccgggatggc gccgctcgaa atttgcacat
     7501 aaagggcggc gccccgccaa agatagaccg gcgcaagctc atcgagcgag gcgttgagct
     7561 tgtggtctat ctttcttcct gggtctcgat tctgggaatt gtctttcttt tcatattcat
     7621 cggcaaggag gcctttccgc tcctcaccga cccggcaatg aaaaaggaag ttcagttcag
     7681 ccagctgtgg acgtcaatct ggcagccggt ctcggatcaa ccgaagttcg gcttgattcc
     7741 tctctttctg ggaacgctca aggc
//
LOCUS       DBNGZA010000436         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1041854, whole genome shotgun sequence.
ACCESSION   DBNGZA010000436 DBNGZA010000000
VERSION     DBNGZA010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1041854"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC16FU_09420"
     CDS             <1..777
                     /locus_tag="AC16FU_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994627.1"
                     /translation="TTSLGDQSSFADNYSATSGLKLGGGFDLDVAYSRRVGTSQTQTS
                     NTRSTSRVFPDLRFSMTNVDRFGLLKKLISSSQLQSNYAFKSDAGLDLNRRHPTARTT
                     TATFQPLAGLTLNWKNGMSTTLQSNQSTAKSQDLSIGSANTRTENTSRGFTLSTSYSF
                     RAPHGIQLPLLRGIRLQSTLRLDLAVTKRRDTQKQAPGGRPLAILTDNSIFNLGLTGN
                     YSFSSQVNGGLQVIWNDTNDLIRKRKSHTRELNFYMELRF"
     gene            781..1698
                     /locus_tag="AC16FU_09425"
     CDS             781..1698
                     /locus_tag="AC16FU_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR2994628.1"
                     /translation="MLHRPRSREILWQSLLFGWFLFWSGGCVSRSLPGFDGRRAYRTL
                     NDFYALGPKVPGTGTHERGLVFLLDTLQPFAAQVDTQQFSLSRGKSEIRLTNVIARFA
                     PEKRERLWLCAHWDTRPRAERDPDSTKRNEPTGGANDGASGVAVLLELARLLHEHPPE
                     VGIDLIFFDGEDFGPEGELDWYFLGSRYFARHLPPQRAELAILLDMVGDRDLAIYREQ
                     NSVRYAPEVVEKVWRKARELGVTTFRDSAKYSVEDDHLQLLWAGIPAIDVIDFDYPWW
                     HTTGDTPDKCSAQSLAAIGRVLAAFIYSR"
     gene            1762..2166
                     /locus_tag="AC16FU_09430"
     CDS             1762..2166
                     /locus_tag="AC16FU_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR2994629.1"
                     /translation="MTLWFTRLQRIAVLVLAVTFLIGAGLWLQKREKTRLEPELIFRT
                     KSITPGPPASLAPETSGTLLPQFQLGQKVDLNTATGEQLAALPGIGPVLAQRIITTRQ
                     KYGPFQKAGDLLRVPGIGRKKLQQIAPLVETK"
     gene            2171..>2653
                     /locus_tag="AC16FU_09435"
     CDS             2171..>2653
                     /locus_tag="AC16FU_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR2994630.1"
                     /translation="MTRFQQKTIGIATGGAAVTYAVVETSGSQRTITDLGAEQAKTKK
                     DPVTAVVKALVARKGNTAAWRNGELRLNLERPAWLNRKVTYPAELAGLYPHWPEWEMR
                     QGLLAAPEGLVIDFWKKALQTGERATAQAVVTRREPVEMTQRILDEAGLECQVFLATP
                     L"
BASE COUNT          556 a          848 c          773 g          476 t
ORIGIN      
        1 acaaccagtc tgggcgacca atccagtttt gcggataact actccgccac cagcggccta
       61 aaactgggcg gcggcttcga cctggacgtt gcctacagcc ggcgggtcgg caccagccag
      121 acccagacca gcaacaccag aagcaccagc cgcgtcttcc cggacctgcg tttcagcatg
      181 acgaacgttg accgtttcgg cctgctgaag aaattgatct ccagctccca actgcagagc
      241 aattatgcct tcaaatccga cgcagggttg gacctgaacc gcaggcatcc cactgccagg
      301 accaccaccg ccaccttcca gcctttggcc ggcttgaccc tgaactggaa aaacggtatg
      361 agcacgaccc tgcagtccaa ccagagcacc gccaaaagtc aggacttgag tatcgggtcg
      421 gccaacacgc gtaccgagaa cacttcccgg ggattcaccc tttccaccag ctattccttt
      481 cgagccccgc acgggatcca actcccctta ctgcggggca tccggctgca gtcgaccctg
      541 aggctcgacc tcgcggtcac caaaagacgc gacacgcaaa agcaggctcc ggggggccgg
      601 ccgctggcca ttctcaccga caactcgatc ttcaatttag gcctgaccgg caactactcc
      661 ttttcctcgc aggtgaatgg cggcctgcag gtgatctgga acgacaccaa cgacctgatc
      721 cgcaagcgca agagccacac gcgagaattg aacttctaca tggagctgcg cttttaagct
      781 gtgcttcacc ggccccgttc gagagagatt ctctggcaga gtctgctttt cgggtggttc
      841 ctcttttgga gtgggggctg tgtttcacga tccctgccgg ggttcgacgg gagacgggcg
      901 taccggacgc tgaatgactt ttacgcttta gggccgaaag tgcccggcac cgggacgcac
      961 gagcgcgggt tagtcttcct gctcgacacg ttgcaaccct ttgccgctca ggtggacacc
     1021 caacaattct ctctctcccg gggtaaaagc gagatcaggt tgaccaacgt tatcgcccgt
     1081 ttcgctccgg aaaaaaggga gcgcctctgg ctctgcgcgc actgggatac ccggccccgg
     1141 gccgaacgcg atcccgacag caccaagcgg aacgaaccga ccggcggggc caacgacggc
     1201 gcctcgggtg tggccgtcct gctggaactg gcgcgccttt tgcacgaaca cccgccggag
     1261 gtgggcatcg acctcatttt ttttgacggg gaagatttcg gtcccgaggg agaactcgat
     1321 tggtactttc tggggtcgcg ctacttcgca cgccacctcc cgccacaacg agcggagtta
     1381 gccattctcc tggacatggt cggcgaccgc gacctggcca tctaccggga gcaaaactca
     1441 gtgcggtatg cgccggaggt ggtggaaaaa gtctggcgga aagcccggga actgggagtc
     1501 acaaccttca gggattccgc gaaatactca gtcgaagatg accacctcca gctgctctgg
     1561 gccggcattc cggccatcga cgtgattgat ttcgactatc cctggtggca taccaccggt
     1621 gacacgcccg ataaatgctc tgcgcaaagc ctggccgcca tcgggcgggt gctggccgcc
     1681 ttcatctaca gccgctgacc tgcttgaccc attggggaga cgggcatacc ataaccgcac
     1741 cttgaggaat atgaggcaac cgtgaccctc tggttcactc gcttgcagcg cattgccgta
     1801 ctggtcctcg cggtaacgtt tttgatcggc gcgggcctct ggctgcagaa gcgggagaag
     1861 acccgccttg agccggagtt gatcttccgc accaagtcca tcacccctgg gcctcccgcc
     1921 tctttggctc ccgaaaccag cgggaccctc cttccccaat ttcaactggg ccagaaagtc
     1981 gacctgaata ccgccaccgg ggagcaactg gccgctctgc cgggcatcgg accagttctg
     2041 gctcagcgga tcatcaccac gcgtcagaag tacggcccgt tccagaaggc cggggatctc
     2101 ttgcgggtgc cgggaatcgg ccgaaagaaa ttacagcaaa tcgccccact ggtcgaaact
     2161 aaatagtagg atgacccggt tccagcagaa aaccatcggc atcgccaccg gcggggcggc
     2221 agtgacctac gccgtggtgg agacctccgg cagccagaga accatcaccg atctcggtgc
     2281 cgaacaggcc aaaaccaaaa aagatccggt cacggccgtg gtgaaagcgc tggtggcccg
     2341 gaaaggaaat accgcagcct ggcgcaacgg ggagttgcgt ttgaatctcg agcgaccggc
     2401 ctggctcaac cgaaaggtga cctatccggc cgagctggcc gggctttacc cccactggcc
     2461 ggaatgggag atgcgacaag gtctgctcgc tgctcccgaa ggcctggtga tcgatttctg
     2521 gaaaaaagcg ctccaaaccg gagagcgggc gacggcgcaa gcggtggtga cgcggcgcga
     2581 gccggtggag atgacccagc gcatcctgga cgaagccggc ttggagtgcc aggtcttcct
     2641 ggccacgcct ctg
//
LOCUS       DBNGZA010000437         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2315812, whole genome shotgun sequence.
ACCESSION   DBNGZA010000437 DBNGZA010000000
VERSION     DBNGZA010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2315812"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="AC16FU_09440"
     CDS             <1..526
                     /locus_tag="AC16FU_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994631.1"
                     /translation="EFTYGNYLLAGVLYQQGKFGLASAAYRPVLKELGQKTRPAYDPE
                     SIFGPEGKTKDLAARLEARAWYGLGASLGALGQLDSAITAFKRALVLWPGFADAQADL
                     GVAFQKQGMAAEAIEALQKAIALDPGKPIYYYNLGVSLATTGRREEARKNFQKTLELD
                     PSFQPARVQLQALR"
     gene            complement(629..>2211)
                     /locus_tag="AC16FU_09445"
     CDS             complement(629..>2211)
                     /locus_tag="AC16FU_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994632.1"
                     /translation="LGIDSTTADSILRTTGLTTDSLYRLAAREVSRVVANPPRWTVVD
                     NINANGSRVAVKLVSLPNGVFVDQVLFLRAFDNFNPPASTPVLYRRYYRNNHPPNTSI
                     EARSDTLFFDRIDTTVVNYCLPETTATWKGINIGWVGSDDIDFPKGGAPLDFSWELYG
                     PFAAKQDAKIDAGKKLDSSYNATTQDPWVTATTRRFVNLQTGWYFFVVRSRDDALVPD
                     TTPATGRFRIIRPTFERPMLLLTYDNRLNPLIQDFGNINAADSALVRNFYYRLLSGSG
                     YLWDAGTDHFVVKRAEGNDSIAFFEEKLSHYQLVVLLYEGIKEPYLNATWTKLFTYLD
                     AGGKVWAMGRNILQYPFEGPFTVPTLKCTDLKTGKPFTYFNLECQYYATSINLGLPGA
                     GKPVNNQFVGAGSLRSELPGLSVDLAKLFRYGTRTAGGRVMWESTRMPDVNYDQRGTK
                     ATPLYVFQVPVAFADTSHLGGTVVAVRYNGPPWQGKPIFKTAHFGFPLYVLDDSGGQV
                     QAMVTEMLRWFLNPAAIP"
BASE COUNT          506 a          608 c          649 g          448 t
ORIGIN      
        1 ggagttcacc tacgggaact accttctggc cggtgtgctc tatcaacagg ggaaattcgg
       61 gctcgccagt gcggcgtacc ggccggtgct gaaggaattg gggcagaaaa cccggccggc
      121 ttacgatccg gagagtatct tcgggcccga aggcaaaacg aaagatctgg ctgcccggct
      181 ggaggcccgc gcctggtatg gcctgggcgc cagcctggga gcgctgggac aactcgattc
      241 ggccatcacc gcattcaaac gggcactggt gctctggccg ggatttgccg acgcccaggc
      301 cgacttaggg gtggcattcc aaaagcaggg gatggctgcg gaagccattg aggcactcca
      361 gaaagcgatt gctcttgacc cggggaaacc aatttattac tacaatctcg gcgtgagtct
      421 cgctacgacc ggccgccggg aagaagcgcg gaaaaacttc caaaagactt tagaacttga
      481 cccatccttt cagccagcga gagtacaatt acaggccttg cgatagttcc tcaagacata
      541 acgaaaaact taagcagaat cgctgctgac taaacaccaa accaaaagcc ccgcctctgg
      601 cggggctttt gcttcccaga aagttacttc agggaatagc cgcgggattc aaaaaccacc
      661 gcaacatctc tgtaaccatt gcctgcacct gacccccgct atcgtcgagc acgtagagcg
      721 ggaacccgaa gtgggcggtt ttgaagatcg gcttaccctg ccagggcgga ccgttgtagc
      781 gcaccgccac caccgtgccg ccgaggtgcg aggtatctgc aaaagccacg gggacttgaa
      841 agacgtataa gggcgtcgct ttcgtcccgc gctggtcgta gttcacatcg ggcatcctcg
      901 tggactccca cataactctt cctcctgcag tccttgttcc ataacggaac agtttggcca
      961 gatcaaccga caagccggga agttcagacc gtagggagcc tgcgccaacg aactgattat
     1021 tgacaggttt ccctgcccct ggcaagccca gattgatcga ggtcgcatag tactggcatt
     1081 ccaggttgaa gtaagtaaac ggcttgcccg tcttcagatc ggtgcacttc aaagtcggaa
     1141 cggtaaatgg cccttcgaaa gggtactgca gaatgttccg tcccatcgcc caaaccttgc
     1201 cgccggcatc cagataggtg aaaagtttcg tccaggtggc gttcagatac ggttccttta
     1261 tcccctcgta caacagcacg actaactgat agtgagagag cttctcttca aagaaagcaa
     1321 tgctgtcgtt cccttccgct ctcttcacga caaagtgatc ggtgccggca tcccagaggt
     1381 acccgcttcc ggagagaagc cggtagtaga aattccggac gagcgcgcta tccgcagcgt
     1441 taatatttcc aaaatcctgt atcaaggggt tcagacggtt atcgtaggtc aataagagca
     1501 taggccgctc aaaggttggc ctgataatcc ggaaccgtcc cgtggccggc gtggtgtcgg
     1561 ggaccaaagc atcgtcccgt gaccggacaa cgaagaagta ccagccggtc tgcaggttga
     1621 cgaagcgtct ggtcgtggcg gtcacccagg ggtcctgcgt ggtggcgttg taggaggaat
     1681 cgagcttttt gcccgcatcg attttggcat cctgtttggc ggcaaacggg ccgtagagct
     1741 cccaggagaa atctaaaggt gcaccgcctt tgggaaagtc gatgtcgtct gagccgaccc
     1801 agccgatatt aatccccttc caggtggcgg tggtttccgg caggcagtag ttgaccacgg
     1861 tggtgtcaat ccggtcaaag aacaatgtat cacttctcgc ctcgatactc gtattcggcg
     1921 ggtggttgtt gcggtaatag cggcggtaga ggaccggggt gctcgccggc gggttaaaat
     1981 tatcgaaggc gcgcaggaag aggacctggt cgacgaatac gccgttgggc agcgaaacta
     2041 atttcacggc cacccgcgag ccgttggcat tgatgttgtc taccacagtc cagcgggggg
     2101 ggttggccac cacccgggaa acttcccggg cggccaaacg gtacagggaa tcggtggtca
     2161 ggcccgtggt gcgcagaatg gaatcggcag ttgttgagtc aatgcccaga a
//
LOCUS       DBNGZA010000438         1801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_191797, whole genome shotgun sequence.
ACCESSION   DBNGZA010000438 DBNGZA010000000
VERSION     DBNGZA010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_191797"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16FU_09450"
     CDS             <1..360
                     /locus_tag="AC16FU_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HIR2994633.1"
                     /translation="YFFYAAVVVLAFLWYDTIKAFIFDGRFGIRIGSLLYLVNIILLS
                     AYTFSCHSFRHLIGGQLDCYSCSASAGARHSLWEKVSLLNHRHMLWAWLSLFSVGLTD
                     LYVRLLATGVVRDRIIF"
     gene            360..>1801
                     /locus_tag="AC16FU_09455"
     CDS             360..>1801
                     /locus_tag="AC16FU_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR2994634.1"
                     /translation="MEAYETVTHDILVIGAGGAGLRAAIAAREQGLSVGLVCKSLLGK
                     AHTVMAEGGVAAALGNVDPQDNWQVHFADTLRGGQFLNNWRMVEIFAREAPERVLELE
                     QWGAAFDRTPDGKIMQRPFGAHTYRRLSHVGDRTGLEMIRTLQDKAVASGVEVYMEVT
                     CTRLVKDGDRVVGAFGYRRQNGRFVLFKARAVILATGGAGKLYKVTSNSWESTGDGVG
                     MAYEAGAELIDIEMVQFHPTGMVWPSGVRGILVTEGVRGEGGILKNNKGERFMQRYDP
                     KKMDLSSRDVVSRAIYTEVQEGRGTEHGGVYLDITHRGADYIKKKLPSMYDQFLALAD
                     VDITREPMEVAPTIHYAMGGVRVDPETGASSLPGLFAAGEVAAGLHGANRLGGNSLSD
                     LIVFGKRAGVGAAVYVKSQPVSTRMAEAQMQAEADLLLQPLQNPGKENPYQLQAELQE
                     TLQNYVGIARNEADLKQALEKIQKLQGRAR"
BASE COUNT          347 a          535 c          553 g          366 t
ORIGIN      
        1 tacttcttct acgccgccgt cgtcgtcctg gccttcctct ggtacgatac gatcaaggct
       61 ttcatcttcg acggtcgctt cggcattcgg atcgggtcac tgctctattt agtgaacata
      121 atcctgctct ccgcctacac tttctcctgt cattcgttcc gtcatttgat cggcgggcaa
      181 ctcgattgct actcttgctc ggcttccgcc ggcgcccgac actccctctg ggaaaaagtg
      241 agtctcctca atcaccgcca tatgctctgg gcctggcttt cccttttttc ggtgggcttg
      301 actgatttat atgtccggct cctggccacc ggcgtcgttc gtgatcggat aatcttctga
      361 tggaagcgta cgaaaccgtc acccacgaca ttctggtaat tggcgccggc ggagccggcc
      421 tgcgggcggc cattgctgcc cgggagcagg gtctttcggt cgggttggtc tgcaagtcgc
      481 tattgggcaa ggcgcacacc gtgatggccg aaggcggcgt ggccgcggcc ctgggtaacg
      541 ttgatccgca ggacaactgg caggtccact ttgctgacac gctgcgcggc ggccagttcc
      601 tcaacaactg gcggatggtg gaaatctttg ccagagaagc gcccgagcgg gtgctggaat
      661 tagagcagtg gggcgcggcg ttcgatcgaa ctcccgacgg caagatcatg cagcgcccct
      721 tcggcgccca cacctaccga cgcctctccc acgtcggcga ccgcaccggc ttggagatga
      781 tccggaccct gcaggacaaa gccgtggcct caggcgtcga agtttacatg gaggtcacct
      841 gcacccgatt agtgaaagat ggtgaccggg tggtcggcgc tttcggctac cgccgccaga
      901 acgggcgctt cgttctcttc aaagcgagag cggtgatttt ggctaccggc ggggcgggca
      961 aactgtacaa ggtgacctcg aactcctggg agagtaccgg cgacggcgtc ggcatggcgt
     1021 acgaggccgg ggccgaatta attgacatcg aaatggtgca attccacccc accggtatgg
     1081 tctggccctc gggggtgcgc gggattctgg tcaccgaagg cgtccgcggc gagggcggta
     1141 tcttgaaaaa caataagggc gagcgcttca tgcagcgcta cgatccaaag aaaatggacc
     1201 tctcctcacg cgatgtggtc tcccgggcaa tttacactga agtgcaggaa gggcgcggca
     1261 ccgagcacgg cggcgtctat ctggacatca cccaccgcgg cgccgactac atcaagaaga
     1321 aattgcccag catgtacgat cagttcctgg ccttagccga cgtcgacatt acccgagagc
     1381 cgatggaagt ggcgccgacg attcactatg ccatgggcgg ggtgcgggtt gatcctgaaa
     1441 ccggcgccag ttccctaccg ggactttttg ccgccggcga agtggccgcc ggactccacg
     1501 gcgccaaccg gctgggtgga aattccctct ccgacctgat tgtcttcggc aaacgggcgg
     1561 gcgttggggc ggctgtttac gtgaagtcac agcccgtttc tacccgtatg gctgaggcgc
     1621 aaatgcaagc ggaagctgac ctgcttttgc agccgttgca gaacccgggg aaggagaatc
     1681 cgtaccagct gcaagccgaa ctgcaggaga cgctgcagaa ctacgtcggc attgcccgga
     1741 atgaagctga cctcaagcag gcgctcgaaa aaatccagaa gttacagggg cgtgcccgga
     1801 a
//
LOCUS       DBNGZA010000439         2336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1890429, whole genome shotgun sequence.
ACCESSION   DBNGZA010000439 DBNGZA010000000
VERSION     DBNGZA010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1890429"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC16FU_09460"
     CDS             complement(<1..420)
                     /locus_tag="AC16FU_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994635.1"
                     /translation="MNTNNILLEVKNVNVYYGGIHALKDVSFQIHQGEIVTLIGSNGA
                     GKTTTLNTISGLLRPKAGEIRFAGEDLTRVAPHETVKRGISQAPEGRKIFANMTVKEN
                     LELGAYLRRDKAGIAADFERVFTIFPRLKERLNQNAGT"
     gene            complement(417..1178)
                     /locus_tag="AC16FU_09465"
     CDS             complement(417..1178)
                     /locus_tag="AC16FU_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994636.1"
                     /translation="MLTLDHVSLSFGGLKAVDRFCLSMNPGELVGLIGPNGAGKTTVF
                     NLITGVYEPDEGAIDFCNCRLNGLKPYQITALSIARTFQNIRLFGNLTVRENVQIACH
                     LHYSSGLPAVLWRSPSSRMAEFEQREKTKRLLEIFELAGVAEEKAKNLPYGQQRRVEV
                     ARALATDPKLLLLDEPAAGMNPQETQKLMQLIRWLKDEFKLTILLIEHDMHVVMGICE
                     RILVLDYGETIAQGPPDAIRKNPRVIEAYLGEEPV"
     gene            complement(1252..2292)
                     /locus_tag="AC16FU_09470"
     CDS             complement(1252..2292)
                     /locus_tag="AC16FU_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR2994637.1"
                     /translation="MLDSTRTGTNLRYWTKQVTFVLGGLALFLLLNNLLAPQLDPYIL
                     RLVILSGINIILAVSLNLINGFTGQFSIGHAGFMAIGGYCAAALTVYGGPSILGALSG
                     LGLPPVAARSILFLVSLLVGGLFAAIAGVLVGIPSLRLRGDYLAIVTLGMGEIIRVII
                     LNTEAIGGARGFACPLYTDLFWVLLWTVLTIVIIYNLLHSSHGRAFLSVREDEVAAEA
                     MGINTTYYKVTAFVIGAFFAGIAGGLFGHYLQLLHTNSFTFMKSFEIVIMVILGGMGS
                     LSGSIIAAVIITLLPEVLRPVKEYRLVLYSALLIVLMLTRPQGLFGTEELSWRRISGW
                     FKRKPKIQPGVA"
BASE COUNT          560 a          622 c          687 g          467 t
ORIGIN      
        1 cgtgccggca ttttgattca gccgctcctt caatcgagga aaaatggtga agacccgttc
       61 gaaatcggcg gcgatgccgg ccttgtcccg gcgcaggtag gcgcccagtt cgagattttc
      121 cttgaccgtc atattggcga aaattttgcg gccttccggc gcctgagaga taccacgctt
      181 caccgtctcg tggggcgcaa cgcgggtgag gtcctcgccc gcgaagcgga tttcgcccgc
      241 tttggggcgc aaaagaccgg agatagtatt gagcgtggtg gttttgcccg cgccgttgga
      301 gccgattaag gtaacaattt ctccctgatg aatctgaaag gagacgtctt ttaaggcatg
      361 aatcccgcca taatagacgt tcacattctt tacttccaat aaaatgttat tagtgttcac
      421 accggctcct cgcccaaata ggcttcgatc actctggggt ttttccggat agcgtccggt
      481 gggccctggg caatggtttc accgtagtcg agcaccagga tgcgctcgca gatgcccatc
      541 accacgtgca tgtcatgctc gatcagaaga atggtgagtt tgaactcgtc tttcagccag
      601 cggatcaact gcatcagttt ctgcgtttcc tgcgggttca tgccggcggc gggctcatcc
      661 aaaagcaaaa gtttcggatc ggtggccagg gcccgggcaa cttccacgcg ccgctgctgg
      721 ccgtagggga ggtttttggc tttttcttcg gctacgccgg ctaattcaaa aatttccaaa
      781 agtcttttgg ttttttcccg ttgctcaaat tcggccattc tacttgaggg agaccgccag
      841 aggacagcgg gcagtcccga cgagtaatgg aggtggcagg cgatctgcac gttttcgcgc
      901 acggtgaggt tcccgaacag gcggatgttc tgaaaggtgc gggcgatgga gagcgcagta
      961 atttgatacg gcttcagccc gttcaatctg caattgcaga agtcaatcgc tccctcatcc
     1021 ggctcgtaga cgccggtgat caggttgaac accgtggttt ttcccgcccc gttggggccg
     1081 atcaaaccca ccagttcgcc ggggttcata ctcaggcaaa agcggtctac cgccttgagg
     1141 ccgccgaagg agagagaaac gtggtcaagg gttagcacag gtaaaagata gtgtcgaagt
     1201 gtcggagtgt cgaggtgtcg gagtaaaaac aagatgcacg cccagaaggg gctaggctac
     1261 gccgggttga attttgggtt tgcgcttgaa ccaaccagat atgcgtcgcc aggagagttc
     1321 ttcggtgccg aagagaccct gggggcgggt gagcatcaaa acaatcaaca aggctgagta
     1381 gagcaccaga cggtattctt tcaccgggcg cagcacctcg ggcaaaagag taataataac
     1441 ggcggcgatg atggagccgg agagcgaccc catcccgccc aggatcacca taatcacgat
     1501 ttcaaacgat ttcatgaaag taaaggaatt cgtgtgcaag agttgcaggt agtggccgaa
     1561 cagcccgccg gcaatgccgg caaaaaaggc gccgatcaca aaggcagtaa ccttgtagta
     1621 ggtcgtgttt atgcccatgg cttcggcggc cacttcatct tcccgcaccg acagaaaagc
     1681 ccggccatgg gaggagtgca gcaggttata gataatcaca atcgtgagca cggtccagag
     1741 gagcacccag aagagatcgg tataaagggg gcaggcaaag ccgcgcgcgc cgccaatcgc
     1801 ttccgtgttc agaataatga cccggatgat ttcacccatc cccagggtga caatggccag
     1861 gtagtcgccg cgcaaacgca gagacggtat ccccaccaga accccggcga tggccgcaaa
     1921 gaggccgcct accagaagac tcaccagaaa caaaatcgag cgtgccgcca ccggggggag
     1981 ccccagcccg gagagggcgc ccaaaattga cgggccgccg taaacggtca aagccgccgc
     2041 acagtaaccg ccaatggcca taaagccggc gtggccgatg gagaactgac cggtgaagcc
     2101 gttaatcaaa ttcaaactga ctgccaggat gatgttgatg cctgagagaa tgaccaatct
     2161 caggatatag ggatcaagtt gcggtgccag gaggttgttg aggagcaaaa agagagccag
     2221 gccgcccaaa acgaaagtga cctgtttggt ccagtagcgc aggttggtcc cggtacgagt
     2281 cgagtccaac acggctacac tttctcgctc accggggaac ccaatagccc ggtcgg
//
LOCUS       DBNGZA010000440         1127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1253852, whole genome shotgun sequence.
ACCESSION   DBNGZA010000440 DBNGZA010000000
VERSION     DBNGZA010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1253852"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="AC16FU_09475"
     CDS             <1..1056
                     /locus_tag="AC16FU_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994638.1"
                     /translation="DEIQSKPEYKELGQFFSFRIQNRIDFKHKTASGKTTNQFQLTNH
                     ELTIYPATGAFAKQLASEVEVSWAADEPLEFENAYVRWVSGTEEGFWSTRLGIFHPFE
                     GYGASDRPVSLARPLFQVSSANYTQNTFFTLWEIDQFGLEAAYNLRRTTFAGTVFNGL
                     FFTGDGLEPAKGGGLQKPAGLRSQNTKDYQLVVNQLLTDNGGGVSAVYYRGTVDLPIT
                     DTTFFQNNFQRAALYGSYPVTDKVVLLLGGALGMDQYYNPATGSTKGRFGSGGGFGEI
                     DLYAHPRATLAGRFDWFDPSDRKKNNEVWAATGAINVPFNNGFQLIWQYQHKQTKVSS
                     PPEKKEDNVQLRAIMIF"
BASE COUNT          252 a          319 c          325 g          231 t
ORIGIN      
        1 gatgaaatcc aaagcaagcc ggagtacaaa gaattaggcc agttcttctc tttccgcatt
       61 caaaatcgga tcgatttcaa gcacaaaacc gccagcggga agaccacgaa ccagttccag
      121 ttgacgaacc acgagctcac catttacccg gccacgggag ctttcgccaa acagctggcg
      181 agcgaagtgg aggtgagctg ggcggcggat gaacccctcg aatttgaaaa cgcctatgtc
      241 cgttgggtct cgggcaccga ggaggggttc tggtccacgc ggttgggaat cttccatccc
      301 tttgagggct acggtgcctc ggatcggccg gtgagtctcg cccgacccct gttccaagtc
      361 tccagcgcca actacacgca gaatacgttc tttacgctct gggagatcga tcagttcggt
      421 ctggaggcgg cgtacaatct aaggcgaacg accttcgcgg ggacagtttt caacggtctt
      481 ttctttacgg gtgacggcct ggagccggcg aagggaggcg ggttgcagaa acccgccggc
      541 cttcggtcgc agaacacgaa agattaccag ctggtggtga atcaactctt aactgacaat
      601 ggcggtggcg tgagcgctgt gtactaccgg ggcacggtcg atttgcccat caccgatact
      661 actttcttcc agaacaactt ccagcgggcg gcgctctacg gatcgtaccc ggtcaccgat
      721 aaggtcgtgc tgcttttagg cggcgctctg gggatggatc agtattacaa tcccgccacc
      781 ggcagcacga aggggcggtt cggcagcggc ggtggctttg gagagatcga tctctacgcc
      841 cacccgcgcg ccaccctggc gggtcgtttc gactggttcg atccgtcgga tcggaaaaag
      901 aataacgagg tctgggccgc caccggcgcc atcaacgtgc cgttcaacaa tggcttccag
      961 ttgatctggc aatatcagca caaacaaacc aaagtcagca gccctccgga aaaaaaggag
     1021 gacaacgttc agctacgagc aattatgatt ttctaagccg gcgacgcggg acgacgacga
     1081 cgttctcccg cgtttcctgt ttgtttccgg gcattcgctt ctaagca
//
LOCUS       DBNGZA010000441         4140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_833993, whole genome shotgun sequence.
ACCESSION   DBNGZA010000441 DBNGZA010000000
VERSION     DBNGZA010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_833993"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..943
                     /locus_tag="AC16FU_09480"
     CDS             287..943
                     /locus_tag="AC16FU_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR2994639.1"
                     /translation="MGQATTSQENYLKTIWELTRQGLVPHGSRVASELGVSPPAVSLA
                     LRRLAREGYVAISGHGNVRLTPKGTKAAEILVRRHRLVEKLLTDVLGMPWHRVHEEAE
                     KLEHAISPEFEKRLGALFAREKTCPHGYPLAVKNSGPAAWAKLKLLSQAKAGERVTIA
                     RVYEKDSAFLLYLDRLAILPGGRAEVVDIAYDETITLRIGQRRVLLGKAAAAKIWVRA
                     "
     gene            1021..2775
                     /locus_tag="AC16FU_09485"
     CDS             1021..2775
                     /locus_tag="AC16FU_09485"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR2994640.1"
                     /translation="MPGICGVFADWPVDRLDKILTGLLTPLASGDHCRSDRFVSPGAA
                     LGRVHLGVFNTAPQPLFSFDRKQALLFYGEIFSGLPAAARDDQSTPQQVLEQLQRGRE
                     PDMAAWNGSFVFASYDFTRKRLLLGNDRYGLRPLYFATFPGGFAFSGSVRSLLSIPAV
                     PRTLNEEALGEFLTLGYLLGNHTLFSGVHLLPPAALLTWEKGPLRVQTYWQADFTQSS
                     RRWRRGEACSQAALLLQQTVERQTRSPKQIGLFLSGGLDSRVLAATARRCGRQLSTFT
                     FDYWNSPERELAARVAQAVQMSNEFLPLTRESYWSQVEAGVRATDGQVNCRNFMGIET
                     NPAVSRKTPVVFSGTGGNAVFGYLSFGILSLLFPWSHETFAARFYRRLATGLNRSELV
                     KLWGPRRGNVLWERGWEGFRRVCAAYRDQPPLVKISRFFFDQMHRRMVFLGLVADHSF
                     LEYRLPLYDYDLVDFMLSMPERERLLARFYRRLLTTHFPEVAVIPYSRTGLPVPAGTS
                     AIFWHKGLQLLLRRAKALPIPLDQELPEEWQKRMEEKFNFLEKTGFQKPVLNQNFSSF
                     SGRKKGLLLTLGIFLDEL"
     gene            complement(2803..3540)
                     /locus_tag="AC16FU_09490"
     CDS             complement(2803..3540)
                     /locus_tag="AC16FU_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR2994641.1"
                     /translation="MTEITLRVFRGDASGGQEKEYHVPAVPGMVVLDAIHYVQTHYDG
                     DLACRWNCKAAKCGSCSAEINGRPRLMCKSRIDEYAGQTIHVRPMKAFPLIKDLVTDV
                     SWNYEVAREIVPFTPPPGERAPFTIYEKDVERVHEFRKCIECFLCQNVCHVLRDHGAQ
                     SQYYGPRFMVKIAALEMHPKDTLKRTDLLRGQGDIGYCNITKCCEELCPEHIHITDNA
                     IIPLKERVADDYFDPLRWLVKKLRGKQ"
     gene            complement(3541..>4140)
                     /locus_tag="AC16FU_09495"
     CDS             complement(3541..>4140)
                     /locus_tag="AC16FU_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HIR2994642.1"
                     /translation="GRRAGLGAAAYVKAQSAPAGVNEAQLQEEADLLLQPLAGPGREN
                     PYQLQSELQETMQNYVGIARNEADLKLAFEKIQKLQGRARSIGVPGSRLYNPAWHTAR
                     DVRFMLVIAEAIIRCALERKESRGAHWRKDFPNKDEQLGRVNFITVKDAGAMKIKTAQ
                     VPSLPPELKRLFEEKPSVSEKLPEPSRGAQPALNPAEKK"
BASE COUNT          762 a         1185 c         1224 g          969 t
ORIGIN      
        1 ggtccatata ccccaccgac accagaaagg cgggccccag gtacccgacg atcttccagt
       61 tccggagcgc ttcccggaga aagccgcgcc ggagaggcgt cttttcgcga atccggcgtt
      121 ctatttcttt ggcagtttcc ggcatcggct gtagcacttg tcagttgtgg gtcccggttg
      181 tatcttttct ctaaccggga gaccgatatt aactatgctt aatattatcc attaatatac
      241 gttaatatcg gcgctgtcaa gcactatttt tagggatttt tttgctatgg gccaggcgac
      301 cacgagtcag gagaactacc tcaagaccat ctgggagttg acccggcagg ggctggtacc
      361 ccacggcagc cgggtggcga gcgaactggg ggtttccccg ccggcggtgt ctttggccct
      421 ccggcggctg gcccgggaag ggtacgtcgc aatttccggt cacggaaacg tccgcctgac
      481 gcccaagggt accaaagcgg cggaaattct ggtgcggcgg caccggctgg tggagaagtt
      541 gctcaccgac gtactgggca tgccctggca ccgggtccac gaggaagcgg agaagttgga
      601 gcacgcaatt tcgccagaat ttgaaaaacg gctgggagcg ctttttgcca gggagaagac
      661 ctgcccgcat ggctatcctt tagccgtgaa aaactccggc ccggccgcct gggcgaagct
      721 gaagctcctc tctcaagcca aagcgggcga gcgggtgact atcgcccgag tttatgagaa
      781 ggacagtgcc tttctcttgt acctcgatcg cttagcaatt ttacccggag ggagggcgga
      841 ggtagtggac attgcctacg acgagaccat cactttgcgg attgggcaac ggcgggtgct
      901 cttggggaag gcggcggcag ccaagatctg ggtgcgagcg tagggacttc cgaaccacaa
      961 gtatttgaga aacctgctct ggcccggact ttttacggag cgcgttaagt aacttttacc
     1021 atgcccggca tttgcggtgt tttcgccgat tggcctgttg atcggctcga caaaatcctt
     1081 accggcttgc tcactccttt ggcatcgggc gaccactgcc gaagcgaccg gttcgtttcg
     1141 cctggcgcgg cgctggggcg ggtgcacctc ggcgtcttca atacggcacc ccaaccgctg
     1201 ttttcctttg accgtaaaca ggcattgctc ttttacggtg aaattttctc aggactacca
     1261 gcggcagccc gggatgatca atcaacgccg cagcaagttc tggaacagtt gcagcgcggg
     1321 agagagccag acatggctgc gtggaatggc tcctttgtct tcgccagcta cgactttacc
     1381 cggaagcggt tgcttttggg gaatgaccgc tatgggttgc gacccctcta ctttgctact
     1441 ttcccgggcg ggttcgcttt ttctggttcg gtccgctcgc ttctgtcaat tcctgctgtc
     1501 ccccgtacgc tcaatgaaga ggctctggga gaatttctga ctctgggata cctcctcggc
     1561 aaccacacgt tgttttccgg ggtccacctg ttaccgccgg cggcactcct gacgtgggaa
     1621 aagggtcccc ttcgggtgca gacctactgg caggccgatt ttacccagtc gtctcggcgg
     1681 tggcgcagag gagaagcgtg ttcccaggcg gctctgcttc tccagcaaac ggttgaacga
     1741 caaacccgct ctcccaaaca gatcggcttg ttcctcagcg gcggtcttga ttcgcgtgtg
     1801 ctggctgcga ctgcccggcg ctgcgggagg cagttgtcca cctttacgtt cgactattgg
     1861 aactcgccgg aacgggagtt ggcagctcgc gtcgcacaag cggttcagat gtcaaatgaa
     1921 ttcttgccac tcacccgtga gtcgtactgg tctcaggtcg aagccggcgt aagggcgact
     1981 gacgggcagg tgaactgccg gaacttcatg ggcatcgaaa ccaacccagc ggtcagccga
     2041 aaaacaccgg tggtgttctc cggaacgggc ggaaacgctg tgttcggata cctttccttc
     2101 ggaattctct cactcctctt tccctggagt catgaaacat tcgctgcccg attctaccgg
     2161 cgtttggcca cgggactgaa tcggtcggag ctggtgaagt tgtggggacc ccgtcggggt
     2221 aatgtgctct gggagcgggg ttgggaaggt tttcgacgtg tatgcgccgc gtatcgtgac
     2281 caaccgccgc tggtgaaaat ctcccggttc tttttcgacc agatgcaccg acggatggtg
     2341 ttcctgggtc tggtcgccga ccattccttc ttggaatatc gccttcccct gtatgactac
     2401 gatctggtcg acttcatgct ttcaatgccg gagcgggagc gactgctggc ccgtttctac
     2461 cggcggttgc tgaccacgca tttcccggag gtggctgtaa ttccctactc ccgtaccggg
     2521 ttgcctgtgc ctgcggggac atcggccatc ttctggcaca agggtctaca gcttctgctg
     2581 cggagagcca aagctctccc aattcctctg gaccaagagc taccggaaga atggcagaag
     2641 cggatggagg agaagttcaa ttttttggaa aaaacaggct ttcaaaaacc cgttttgaat
     2701 caaaattttt cttcgttctc cggaagaaaa aaaggactcc tgctgactct gggtatcttt
     2761 ttagatgagc tctaaggggg taaccttctc tgcggggttc agctactgct tcccgcgtag
     2821 cttcttcacc agccaccgca gcgggtcgaa gtagtcgtcg gcgacgcgct ccttcaaggg
     2881 gatgatagcg ttgtcggtga tgtggatgtg ctccgggcag agctcctcgc agcacttggt
     2941 gatgttgcag taaccgatgt cgccttggcc ccgcaaaagg tcagtccgct tgagcgtgtc
     3001 tttcgggtgc atttccagtg ccgcgatttt caccataaag cgcgggccgt agtactggga
     3061 ctgtgccccg tggtcgcgca ggacgtggca gacgttctgg cagagaaaac actcgatgca
     3121 cttgcggaac tcgtgcaccc gctccacgtc cttttcataa atcgtaaacg gcgcccgctc
     3181 tcccggcggc ggggtgaaag gcacaatctc tctggctact tcgtagttcc aggaaacgtc
     3241 ggtcactaag tcttttatga gtgggaacgc tttcatcgga cggacatgga tcgtttggcc
     3301 ggcatactcg tcgatgcggc tcttgcacat caaccgcggt cgcccgttaa tttccgccga
     3361 acaggagccg cacttggccg ccttgcagtt ccagcggcag gccagatcgc cgtcgtaatg
     3421 ggtctggacg taatgaatgg cgtctaaaac taccatcccc ggcacggcgg ggacgtggta
     3481 ctctttctcc tgcccgccgg aggcatcacc ccggaagacg cgcagcgtga tttcggtcat
     3541 ttattttttc tccgcggggt tcagggccgg ttgagctccc cgcgacggtt cgggcagctt
     3601 ttcggaaacc gaaggttttt cttcgaaaag gcgcttgagt tcaggcggca gggacggcac
     3661 ctgtgccgtt ttgattttca ttgcgccggc atccttgacg gtgatgaaat tgacccggcc
     3721 caattgctcg tctttattcg ggaaatcttt ccgccagtgc gccccccggc tctctttgcg
     3781 ttccagggca cagcggatga tggcttccgc aatgacgagc atgaagcgaa cgtcacgggc
     3841 ggtgtgccag gcggggttgt acagtcgcga gcccggaacc ccgatgctcc gggcccgccc
     3901 ctgtaacttt tggatttttt cgaacgccag cttgaggtca gcttcgttcc gggcaatgcc
     3961 gacgtaattc tgcatcgtct cctgcagttc actctgcagc tggtaggggt tttccctccc
     4021 cggaccagcc agcggctgca agagcagatc ggcttcttct tgcagctggg cctcattcac
     4081 tccggcggga gcggactgcg ctttcacgta cgccgccgcg cccagcccgg cccgtcggcc
//
LOCUS       DBNGZA010000442         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_408002, whole genome shotgun sequence.
ACCESSION   DBNGZA010000442 DBNGZA010000000
VERSION     DBNGZA010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_408002"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="dacB"
                     /locus_tag="AC16FU_09500"
     CDS             <1..963
                     /gene="dacB"
                     /locus_tag="AC16FU_09500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR2994643.1"
                     /translation="GWTWRDLSYWYATSTGALSYSDNCVALFFSPGPKVGAPAKIKID
                     PQTDYLTVINQTKTGPRRTRRSIDYTRKPGTNVVTFFGQMGTRDTAEYKDYVSVEEPG
                     KYLATVFRQVLSSRRISVRGTIASAADDSAAVARAIPLFVWNSAPLAEILKVVNKNSQ
                     NFYAEQILKTVGREKRGEGSFAAGLAAESTFLLAVGIDSTQFDLSDGSGLSSDNLVTA
                     DALVRLLVFMAHHPNRNEFYESMAIPGIDKTVKLRLNDVPQADQMRVKTGFIRHVSAL
                     SGYLAALNGKGYAFSLLVNNYRGDLRRIWAWQDRFCRAVIEGAP"
     gene            966..>1686
                     /gene="alr"
                     /locus_tag="AC16FU_09505"
     CDS             966..>1686
                     /gene="alr"
                     /locus_tag="AC16FU_09505"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR2994644.1"
                     /translation="MGRNSSNNRYHRPAWVEVNLKQLAQNVRVIKKAIGPCSQLMAVV
                     KADAYGHGAIPVAKMTLKAGAKLLGVALLEEGVALREAGVRAPIVVLYPEAAERAPYY
                     VPYGLMPTICDASFGQHYAQALKKRGRRSEFYIKVETGMHRYGLPVNQNLALLQRLKN
                     FPALRLAGAFSHFADSGEDGDYSQVQLARFRSALSAFAQAGFPPPLASIANSGALLEV
                     SHSALDMTRIGILLFGVPPVPH"
BASE COUNT          328 a          525 c          495 g          338 t
ORIGIN      
        1 ggctggacct ggcgcgacct ctcctactgg tatgccacct ctaccggtgc gctttcgtac
       61 agtgacaact gcgtggcgct cttcttctct cccggcccga aagtgggggc gccggcgaag
      121 atcaaaatcg atccgcagac cgattacctc accgtcatca accagacgaa gaccggcccg
      181 cgccgaacac gccgttccat cgactacacc cgcaaaccgg gcacgaacgt tgtcactttc
      241 ttcggacaga tggggacgag agacaccgcc gagtacaagg attacgtttc ggtggaagag
      301 ccggggaaat atctggcgac ggtgttccgc caggtcctga gttcgcgccg catttccgtg
      361 cggggcacta ttgcttctgc cgctgacgac tcggcggcgg tggcccgggc gattcccctc
      421 tttgtgtgga attcggcgcc tttggccgaa attctgaaag tggtgaacaa gaacagccag
      481 aacttctacg ccgagcagat cctcaagacc gtggggcggg aaaagcgggg ggagggaagt
      541 ttcgccgcgg gactggccgc ggagagcacc ttccttctgg ccgtcggcat cgactccacg
      601 cagtttgatc tctccgacgg ttcgggcctt tccagcgaca atctcgtcac cgccgacgcc
      661 ctggtacgac tcttagtttt catggcgcac cacccgaatc gaaatgaatt ttatgaatca
      721 atggccatac ccggcatcga taagaccgtc aaactccggc tgaacgacgt tccccaagca
      781 gaccagatgc gggtcaagac cggcttcatc cggcacgtct cagctctctc cggatacctc
      841 gcggctctca acggcaaggg gtacgccttt tcgcttctcg tcaacaacta ccggggcgac
      901 ctgaggcgga tctgggcctg gcaggaccgc ttctgccggg cggtcatcga gggagcgccg
      961 tagcaatggg ccggaactct tccaacaacc ggtatcaccg gcctgcctgg gtcgaagtta
     1021 acttaaagca actcgcgcaa aacgttcggg tcatcaaaaa ggcaattgga ccgtgctcgc
     1081 agttgatggc cgtggtcaaa gccgatgcgt acgggcacgg agccataccc gttgccaaaa
     1141 tgacgcttaa ggccggagcg aagcttctgg gcgtggcgct tttagaggaa ggggtggcgc
     1201 tccgcgaagc cggggtccgg gcgccgatcg tggttctcta ccccgaagcg gcggagcgcg
     1261 caccctacta cgtgccgtac ggcctcatgc ccaccatctg tgatgcctct ttcggtcagc
     1321 actacgctca agcattgaag aagcgtggtc gccgcagcga gttctatata aaagtcgaga
     1381 ccggaatgca ccgctacggg ctaccggtca atcagaatct ggcgcttctg cagcggctga
     1441 agaattttcc cgccctgcgc ttggccgggg cattctcgca ctttgccgat tcgggagagg
     1501 acggagacta cagccaggta caactggctc gcttccgcag tgcgctttcg gcctttgccc
     1561 aagccggttt tccgccgcct ctggcttcca ttgccaatag cggggcgctg ctcgaagtct
     1621 cccattcggc tctcgatatg acgcggatcg gcattttgct cttcggcgtg ccgcccgtgc
     1681 cgcatc
//
LOCUS       DBNGZA010000443         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_937056, whole genome shotgun sequence.
ACCESSION   DBNGZA010000443 DBNGZA010000000
VERSION     DBNGZA010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_937056"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="AC16FU_09510"
     CDS             <1..892
                     /locus_tag="AC16FU_09510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994645.1"
                     /translation="DDHPTRDGDLVRKGLTLFRQGKLSQAEALLRQALAANGRDPELH
                     NNLGVILYSQKRYPEALEFFRTSIRNGPQYKDAYYNLAQLYLTTDRRQEAQATLQQYL
                     SLNGGDRDVELLLGSLFISGGEWQKASELLEKVVTNSPDNLLALNNLGLAYFNLQKTG
                     LAKAVWQKVVARDPKNVDALFALALVCQKEKAHTEALGYFEACLRLDPAKAEVYNHYG
                     VSLCHTARIAEAEEVFRQAVTFKPDYLNAWVNLGALYQVTGRLGQAKEAYEKAKALAP
                     NEPVIEEQLAELQKRLQSED"
     gene            1053..1751
                     /locus_tag="AC16FU_09515"
     CDS             1053..1751
                     /locus_tag="AC16FU_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR2994646.1"
                     /translation="MKQLALNCMVCGQRPKGIFCTLAGSHLTRLDREKTVHEYQRGQV
                     IFYEGNPAGAIHCIYSGRVKLYKTGPKGDYQIIRLLGPGEIMGYRALLANEPCAATAE
                     AVEPTTICTISKETLFDLLRQSSELAFQLLTKLAKELRVSEEQELVIAQETVRQRTAR
                     FLLFCCGGEKPKVGEPLKCPLSRAEMAQMVGTTPETFSRTLRYFALRGILRVTRWEIF
                     IENPAALELLSRSL"
BASE COUNT          472 a          554 c          559 g          412 t
ORIGIN      
        1 ggacgatcat ccgacgcgcg acggggatct ggtccgcaaa ggattgacgc tttttcgcca
       61 gggaaaactc tcccaggcgg aggcgctttt gcggcaggct ttggctgcca atggccgcga
      121 cccggagttg cacaacaacc tcggcgtcat tctctactcc cagaagcggt atccggaagc
      181 cttagagttt ttccggacca gcatccggaa cgggccgcag tataaagacg cgtactataa
      241 cttagcgcag ctgtacctca ccaccgaccg ccgccaggag gcacaggcga cgctccagca
      301 gtatctttcc ctgaacggcg gagaccggga cgtcgaacta ctcctgggga gtctattcat
      361 ttccggtgga gagtggcaaa aggcatctga acttttggaa aaggtggtga ccaactcccc
      421 ggacaatctt ctggcgctga acaacctcgg tctggcctat ttcaatttgc agaagaccgg
      481 cttggccaaa gcggtctggc aaaaggtggt ggcgcgtgac cccaagaacg tcgatgccct
      541 gtttgcgctg gcgttagtct gccagaaaga gaaagcgcat acggaagccc tcggctactt
      601 tgaagcctgc ctgcggctgg atcccgcgaa agccgaagtg tacaaccact acggggtcag
      661 tctctgccat acggcccgca tcgccgaagc cgaggaggtg ttccgtcagg cggttacctt
      721 taagcccgac tatctcaatg cctgggtgaa tctgggggca ctctaccagg tcacgggacg
      781 cctgggacag gccaaggagg cctacgaaaa agctaaggcc ctggcgccga acgagccggt
      841 gattgaagaa cagttagccg aactgcaaaa acggctgcag tcagaagatt agctcacacc
      901 tctgtcggcc aatttcgtcg tacctctctc aatacaacga ataattcgta accggtttca
      961 attgacaacc acctcggtct cctatttttt gtccgaatga ataccttctt cagatgtcga
     1021 tctttcgact ggccttctga gccggtagaa aaatgaaaca gttagctctc aattgcatgg
     1081 tctgcggcca gcggccgaaa ggtatcttct gcactctggc aggttctcac ttgacccggc
     1141 ttgatcgcga gaaaaccgtc cacgaatacc aacgggggca ggttattttc tatgagggta
     1201 atccggctgg agcgattcac tgtatctact cggggcgggt gaaactctac aaaacgggac
     1261 cgaaaggcga ttaccagatc attcggctcc tgggaccggg ggagattatg ggttaccggg
     1321 ccttgttggc caatgagccc tgtgccgcca ccgcggaagc cgttgagcca accaccatct
     1381 gtaccatctc gaaggagact ctttttgatc tcctgaggca atcatccgaa ctggccttcc
     1441 agcttttgac caaactggcc aaagagctgc gcgtctccga agagcaggag ttggtcatcg
     1501 cccaggaaac ggtgcggcag cgcaccgccc gctttctgct cttttgctgt gggggagaga
     1561 agccgaaagt aggggaaccg ctcaagtgcc cactttcccg ggctgaaatg gcccagatgg
     1621 tggggacaac gccggagaca ttctcccgca ccctgcgcta ctttgccctg cggggcatcc
     1681 tgcgcgtgac ccgctgggag atcttcatcg aaaatcccgc cgccttagag cttttatccc
     1741 gctcgctcta atcccgactt gacgccgatc aagccggcgc ttgacaccca tcattggaat
     1801 taaaagagta ttatatattt tcatacttga ttcgtctcca ttctgtacga ccgcaccgaa
     1861 ccagcacagc cgaccataag aggatgcgat gcggccagtg gaacaataga ggggaggaag
     1921 aaggtatgac agcgagcgga gggtaaaaag cagaatgtct gtgagcagag gaagtaaaca
     1981 aggtgacggg aaggaga
//
LOCUS       DBNGZA010000444         3509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2634331, whole genome shotgun sequence.
ACCESSION   DBNGZA010000444 DBNGZA010000000
VERSION     DBNGZA010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2634331"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC16FU_09520"
     CDS             complement(<1..233)
                     /locus_tag="AC16FU_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR2994647.1"
                     /translation="MPIPIVAIIGRPNVGKSTLFNRLVKRRVAVVHDLPGVTRDRNYF
                     LASWNRKQFYLVDTGGLVPQAQSDIERLVKEQA"
     gene            complement(321..1595)
                     /locus_tag="AC16FU_09525"
     CDS             complement(321..1595)
                     /locus_tag="AC16FU_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR2994648.1"
                     /translation="MPDEQLRMFVFLSELLYRRVVDEKLRPVGKVADFKVGLRELFPK
                     IEALVLRQHGTKTPLALSWELVKSVNGHQIVVTEEAGQKLGPLATAADELLLRDEILD
                     KQVVDTAGAKLERANDLHLLINDGELRLVHVDVGLRGILRRLGWTRAFDAVTNWLFAY
                     QIPQKLISWKYIQPLAHDPEKKRLKLNVTLRKLSEIHPADLADIIEELDKHKASAVFQ
                     TLDVESQAEALEELDPKLQKTLIEQVDPSRASDIIEEMAPDEAADLLADISEEKKEVV
                     IRGLEAESRTELTELLTYPEGTAGALMTKDFFCARAGSTVAQAIELLKTLHAEVEMFY
                     YAYVVDENGRLVGALSLRNMLLHDPQAKLEEIMHRHLITVRLTNKQKTVFKKFQKYDY
                     LALPVVDDKGILKGIVTLKDALAAVHPEIVGK"
     gene            complement(1595..1771)
                     /locus_tag="AC16FU_09530"
     CDS             complement(1595..1771)
                     /locus_tag="AC16FU_09530"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR2994649.1"
                     /translation="MGRRGGEIWFPLILIVLGGIFLLKNLGVVSVSLHRWWPVIFILW
                     GASILLRRSTRNNT"
     gene            complement(2118..3155)
                     /locus_tag="AC16FU_09535"
     CDS             complement(2118..3155)
                     /locus_tag="AC16FU_09535"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR2994650.1"
                     /translation="MKPLYHYVGAIHIHTTYSDGSKPIAEVIAIGRNAGLDFMLFSDH
                     MTLKGLEEKKEGWYDRTLVLIGYEINDREDRNHLLAFNLRQVLPADLKPKEYVRKVRE
                     HGGLGIIAHPDEVRNKLPQYRPYPWNAWDVDGFDGIEIWNQMSEWMEKLTRWNRLAMV
                     FRPRRSLYGPTPRILARWDEYNQKRPTVGTGGIDVHAHPHKFGPFTIIIFPYEVQFKS
                     IRTHVLLDKPLSKEFPEAKRQLLEAIRHCRVFISNYRWGEAMNFRFWAENGGDEVTIG
                     ETISLKKSVHLHAHFPRPCESRLIHNGQLATTAQGMVLHYEAKLSGVYRIEAYRKGRG
                     WIYSNHIRVSG"
     gene            complement(3152..>3509)
                     /locus_tag="AC16FU_09540"
     CDS             complement(3152..>3509)
                     /locus_tag="AC16FU_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994651.1"
                     /translation="LGSTWTSARPLLASLVPAKRVGQFFGLYALSGRAAAILGPLVWG
                     FCVLYFKVDSAIVQAMISGLERLGVTFSPQVLPSIQYRIAIVALAMLMAVGLILFRKV
                     PDRFQKRVKLSVEPHQ"
BASE COUNT          751 a         1029 c          952 g          777 t
ORIGIN      
        1 tccgcctgct ctttgaccag ccgttcgatg tcggactgcg cctgcggcac caagccgccg
       61 gtgtcgacca aatagaactg cttgcggttc caggaagcga gaaagtagtt ccgatcgcgg
      121 gtgacgccgg gcagatcgtg caccaccgcc acccggcgct tcaccagccg gttaaagagc
      181 gtcgatttgc caacattggg ccgaccgatg atcgcaacga ttgggatagg cataaccaag
      241 gaccgcttcg gggaaatata ctgcgctgcc ttgagaaaat ctccgaaaaa acgaactccg
      301 gcacaaggga gagcggaaga ctacttcccg acgatctccg ggtgcactgc cgccaaagcg
      361 tctttgaggg tgacaatccc tttcaggatg cctttgtcat ccaccaccgg cagggccagg
      421 tagtcgtact tctggaactt cttgaagacg gtcttctgct tgttggtcag acgaacggtg
      481 atcaaatgcc gatgcatgat ctcttccagt ttggcttgcg gatcgtggag caacatgttg
      541 cgcagggaaa gtgcacccac caagcgtccg ttctcatcca cgacgtaggc gtagtagaac
      601 atctccactt cggcgtggag ggttttgagc aactctatcg cttgcgccac cgttgacccg
      661 gcccgggcgc agaaaaaatc tttggtcatc aaagctccgg ccgtgccttc gggataggtc
      721 aagagctcgg tcaactccgt ccgcgactcg gcttccaacc cccggatgac cacctctttt
      781 ttctcttcgg agatgtcggc taagaggtcg gccgcttcgt cgggcgccat ttcctcaatg
      841 atgtcggagg cgcgggacgg atcgacttgc tcgatcaagg tcttttgcag ttttggatcg
      901 agctcctcca acgcttcggc ctggctttcc acatccaacg tctgaaacac ggccgaggct
      961 ttatgcttgt ccagttcctc aatgatgtcg gccaagtcgg ccgggtggat ttctgaaagc
     1021 ttgcgcaggg tgacgttcaa cttcagtcgc ttcttctccg ggtcgtgcgc caagggctga
     1081 atgtatttcc aggagatcaa cttctgcgga atctggtagg caaagagcca gttggtgacc
     1141 gcatcgaacg cgcgcgtcca gcccaggcgc cggaggatgc cccggaggcc cacgtcgacg
     1201 tgcaccagcc gcagttcgcc gtcgttgatg agtaagtgca ggtcgttcgc gcgttccagt
     1261 tttgctccgg ccgtatcgac cacctgcttg tccaaaattt catcgcgaag caaaagttcg
     1321 tctgcagcag tggcgagcgg tcccagtttc tggccagcct cctcggtgac gacgatttga
     1381 tgcccgttga cgcttttgac cagctcccac gagagtgcca ggggtgtttt cgtaccgtgc
     1441 tgccggagga cgagcgcctc aattttgggg aagagttccc gcaaacccac cttgaaatcg
     1501 gccaccttcc ccaccgggcg gagcttttca tccaccaccc gccggtagag aagctccgac
     1561 agaaagacaa acatccgcag ttgttcgtcg ggcatcaggt attgttcctc gtgcttcgac
     1621 ggagaagaat cgacgcgccc cagaggatga agatcaccgg ccaccagcgg tggagcgaca
     1681 ccgacaccac tcccagattt ttcaaaagaa aaatgccacc cagcacaatc aaaatcagcg
     1741 gaaaccagat ttcaccgccg cgtctgccca cccagacctc ctttgttaag aggtaagata
     1801 gggccacaga aatcgcggtc aatggttttt ctcgctggta gcataggtcc tcgtggccgt
     1861 tttttttaga aaaacggggt atgcccctcc gctatccggt tcattgttat ggaattgtgg
     1921 tacaaacctt ctatgaacgc gatcgtattg acccctccct caccctcctc ggtacgagtc
     1981 ttcgaccctc aagggaggga agccctttac cttctctccg taaggctgat tactccctct
     2041 cctgtagtgg ttcgatccgc ttcaggcgga ctcaccaccc tgaaattgtc gagggagagg
     2101 gataaagggt gaggtctcta ccctgacact cgaatgtgat tcgaataaat ccatcccctc
     2161 cccttccggt acgcctcaat ccggtagacc ccagataatt ttgcttcata gtgcaacacc
     2221 atcccctgcg cggtggtggc cagttgtcca ttgtgaatta aacgcgattc gcagggccgg
     2281 gggaagtgcg cgtgcaagtg caccgacttc tttaacgaaa tggtctctcc tattgtgacc
     2341 tcgtcccccc cattctccgc ccaaaagcga aagttcatcg cctcccccca ccggtagttc
     2401 gaaatgaaca cccggcagtg ccggatggct tccagaagtt gtcttttggc ctccgggaat
     2461 tcttttgaaa gcggtttgtc gagtaaaacg tgggtgcgga ttgacttgaa ctgcacctcg
     2521 taggggaaaa tgataatggt gaacggcccg aatttgtgcg ggtgcgcatg aacgtcaatg
     2581 ccgccggtgc ccaccgtcgg acgcttttgg ttatattcat cccagcgcgc taagatgcgc
     2641 ggcgtagggc cgtagagcga gcgccgtggt cgaaagacca tcgccagccg gttccagcgg
     2701 gtcaacttct ccatccactc cgacatctgg ttccagattt caatcccgtc gaagccgtcg
     2761 acatcccagg cgttccaggg gtagggccgg tactgcggca gtttattgcg cacctcgtcc
     2821 gggtgagcga taatccccag accgccgtgc tcgcgcactt tccggacgta ctccttcggc
     2881 ttcaaatcgg ccggcagcac ctgcctgaga ttaaatgcca gcaggtgatt gcgatcttct
     2941 ctatcattga tttcatatcc gatcaatact aaggttcggt cgtaccagcc ctcttttttt
     3001 tcctccagcc ctttcagggt catgtgatcg gagaaaagca tgaagtccaa ccccgcgttc
     3061 cggccgatgg caatcacttc cgcaatcggc ttgctgccat ccgagtaagt ggtgtggatg
     3121 tggatggctc cgacgtagtg atagagtggt ttcattgatg gggttcgact gataatttca
     3181 cccgtttctg gaaacggtcc ggcactttgc ggaacaggat caggccgaca gccataagca
     3241 ttgccagcgc gacgatggca atccggtact ggatggaggg cagcacctgc ggcgaaaagg
     3301 tgacgcccag acgttccagg ccggaaatca ttgcctgcac gatagccgaa tccactttga
     3361 aatacaagac gcagaagccc cagaccagag gacctaaaat cgccgccgcc cggcccgaaa
     3421 gggcgtacaa tccgaagaat tgccctaccc gcttggccgg cacgagactg gccaaaagcg
     3481 gccgggccga agtccaggtc gaaccgaga
//
LOCUS       DBNGZA010000445         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1784715, whole genome shotgun sequence.
ACCESSION   DBNGZA010000445 DBNGZA010000000
VERSION     DBNGZA010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1784715"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="AC16FU_09545"
     CDS             <1..196
                     /locus_tag="AC16FU_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994652.1"
                     /translation="ENTILTYTLLGEKGKVYEWMTKGMEANKIDWLFLEFNPLLEELR
                     HEPEFQHHLQEIKKRVLNSE"
     gene            266..1540
                     /locus_tag="AC16FU_09550"
     CDS             266..1540
                     /locus_tag="AC16FU_09550"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HIR2994653.1"
                     /translation="MPDLLREENQKLKQAVEELSLLNEIATAVSSTMPMEEINKLIVG
                     KCLSRIKAEQGYISLVENQEKTDRTKTLVRVSEKSAAVLPFRLGVTLTGWMVKSQVPL
                     LVNDALADERFGGSEWNGIPIRSVLAVPLKVKNQLIGFLALFNKKEGAFTPEDQRLLS
                     IVGMQAAQTIEAARLYAEEKKVAALEEDLRTAKIIQQNLLPKSTPQIPGFDVAGMTVP
                     AKEVGGDYFDYLPLPDGRWGFVVADVAGKGLPAAMLMSNLQATIRGQSAVSASCAQCI
                     ATANRMLMLSVGTGRFVTVFLAALDGARKMLSYSNGGHNRPYLFKPDGAFRELATGGP
                     LLGVVDSLPYEEEAVPLAPGDVLVIYSDGVTEAFNEKGEQFGEKRLVETLKPVLSYSA
                     AEILAAVTNAVHSFAGNTPAADDLTLLVVKAV"
     gene            complement(1590..2987)
                     /gene="purF"
                     /locus_tag="AC16FU_09555"
     CDS             complement(1590..2987)
                     /gene="purF"
                     /locus_tag="AC16FU_09555"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR2994654.1"
                     /translation="MENSLTEKCGVFGVYGARNAAELIYFGLYSLQHRGQECAGIVTV
                     NDGRLHLRKGMGLVNDLFPSRDFLSDLKGSFGIGHNRYSTTGSSNEINIQPLLMAYRD
                     RQLAVGHNGNLTNSISLKASLEKSGAIFQTTSDTELILHLVARSKKGKPVQKICEALT
                     KVQGAYSLVFATNDSIIAARDPMGFRPLALGRLGRAHVVASETCAFDLIGAKYERDVE
                     PGEVLEISAKGLKSYQPFKSQPHAFCIFEFIYFARPDSMIFGENVDKVRRRLGRQLAI
                     EHPVEADIVTSVPDSSNTAALGFSEQSKIRYEIGFIRNHYVGRTFIQPSQEIRDLDVK
                     VKFNPVKGVLNDKRVVIVDDSIVRGTTSKKLVAMVREAGAKEVHFRVSCPPIVSPCFY
                     GIDMPTKRELIGSYKTVEEIRRFLGVDSLGYLSIESMLSMSSLPRGETFCTACFSGKY
                     PTKIENNIGKLSLEN"
BASE COUNT          736 a          922 c          925 g          694 t
ORIGIN      
        1 cgaaaatacc atcctgacct acacccttct gggcgagaag ggtaaggtgt atgagtggat
       61 gaccaagggg atggaggcca ataaaattga ttggctcttc ctggaattca atcccctcct
      121 ggaagaactg cggcatgagc cggaatttca gcaccatttg caggaaatca aaaagagagt
      181 actgaactca gagtaattcg cctccccgca caacacgccc tctgatagtt gcccccccgc
      241 ctttgctcca gcatatttca ctcctatgcc cgatttgctc cgggaagaaa accagaaact
      301 caagcaggcg gtggaggaac tttcgctttt gaatgaaatc gccactgcag tgtcctccac
      361 catgccgatg gaggagatca ataaactgat cgtcggcaaa tgtctctccc gcatcaaagc
      421 cgagcagggg tacatttcgc tcgtcgaaaa ccaggagaaa accgaccgca cgaaaacgct
      481 ggtgcgggtc agtgagaaat ctgccgccgt gctccccttc cggctggggg tcaccctgac
      541 cggctggatg gtgaaaagtc aggtgccgct tttggtcaac gacgccttgg ccgacgagcg
      601 ctttggcggg agcgaatgga acggtatccc gatccgctcg gtcttggccg tcccgctgaa
      661 agtgaaaaat caactcatcg ggtttctggc gctcttcaac aaaaaagagg gagcgttcac
      721 cccggaagac cagcgcttgc tctccatcgt cgggatgcag gcggcgcaga ccatcgaagc
      781 ggcgcggctg tacgcagaag agaaaaaagt tgcggcgttg gaagaagacc tgcgcacggc
      841 caagatcatc cagcagaatc ttttgcccaa gtccacgccc caaatccccg gctttgacgt
      901 ggccggaatg acggtgccgg ccaaagaagt gggcggcgac tacttcgatt acctccccct
      961 cccggacggg cgctggggct tcgtcgttgc cgacgtggcc ggcaagggcc tgcccgccgc
     1021 catgctcatg tcgaacctgc aggccaccat ccgggggcag tcagcggttt cggcctcctg
     1081 cgcccagtgt attgccactg ccaaccgaat gctcatgctc tcggtcggaa cggggcgttt
     1141 tgtcaccgtc ttcttagctg ctttagacgg ggcccgcaaa atgctctcct actccaacgg
     1201 cgggcacaat cggccctatc tctttaagcc ggatggagca tttcgcgaac tcgccaccgg
     1261 cgggccgctt ttgggggtgg ttgattccct gccctatgag gaagaagccg tcccgcttgc
     1321 gccgggcgac gtgctggtaa tctattccga tggcgttacg gaagcgttca atgaaaaagg
     1381 agaacaattc ggggaaaagc ggctggtgga aacactaaag ccggtgcttt cctattcggc
     1441 ggctgaaatt cttgccgccg ttaccaacgc cgtccactcc tttgccggca acacccccgc
     1501 cgcggatgat ttgaccctgc tggtggtgaa ggcggtgtga aaaaaagact tttaatacta
     1561 tgctggcagt aacttctaac gcagcaaatt tagttttcca gcgataattt cccgatattg
     1621 ttctcaatct tggtcggata cttccctgaa aagcaggcgg tgcagaaagt ctcgccccgg
     1681 ggcagagacg acatcgagag catgctttca atcgagaggt agcccaggga atcgacaccg
     1741 agaaacctgc gaatctcctc caccgtcttg tatgaaccaa tcaactcccg cttggtcggc
     1801 atatcaatcc cgtagaagca gggggagaca atcggcgggc aggagacgcg gaagtggact
     1861 tccttcgccc ctgcctcccg caccattgcc acaagttttt tggaggtcgt cccgcgcacg
     1921 atggaatcgt ccacaattac cacccgcttg tcgtttaaga cccccttaac cgggttgaac
     1981 ttcaccttca catctaagtc acgaatctcc tgcgacggct ggatgaaagt ccgccccacg
     2041 tagtggttgc ggatgaagcc gatttcgtag cggattttcg actgctcgga aaagcccaga
     2101 gcggcggtgt tggaggagtc gggcaccgag gtcacgatgt cggcttccac cgggtgctcg
     2161 atggccaact ggcggcccag ccggcggcgg actttatcga cgttttcgcc gaaaatcatc
     2221 gagtcgggcc gggcaaagta gatgaactca aaaatgcaaa aggcgtgcgg ttgagacttg
     2281 aacggttggt acgatttcaa ccccttagcc gaaatctcta acacctcacc cggctccacg
     2341 tcccgttcat acttcgcgcc aatcaggtca aaggcgcagg tctcggaggc gacgacgtgt
     2401 gcccggccca gccgtcccaa ggccagcgga cggaagccca tcgggtcgcg ggcagcgatg
     2461 atcgaatcgt tcgtggcaaa gacgagcgaa taagcgccct ggactttcgt aagcgcctcg
     2521 caaatcttct gtaccggttt tcctttcttt gagcgggcga ctaagtgcag aatcagttcg
     2581 gtatccgacg tggtctggaa aatggcgccc gatttctcca gcgacgcttt caaagaaatc
     2641 gaattggtca ggttcccatt gtgccccacc gccaactggc ggtcccggta ggccatcaaa
     2701 agcggctgga tgttaatctc attggaggag ccggtggtgg agtagcggtt gtgcccgatg
     2761 ccgaaggaac ctttgaggtc ggagaggaaa tcgcgcgagg ggaagaggtc gttgaccaga
     2821 cccatcccct tacgcaggtg caaacgcccg tcgttgacgg tgacaatccc cgcgcactcc
     2881 tggccccggt gctgaaggga gtagaggccg aagtagatca actcggccgc attccgggcg
     2941 ccgtagacgc caaagacgcc gcacttttcg gtcaaggaat tttcaatcac cgctcggcga
     3001 cacccttcag atcggcttaa ggatcaggct ccactcgact ggcttcccac cctctgtggg
     3061 taagtttata cggcaggaat ctacgggcga tggagtccct gtcaagccga tgaaattggg
     3121 tcttttgcag ggaaggtgaa aagtcgttca aagttgttgc ccggggcgcg ttttttttgt
     3181 aagttcccca cagccgccga tttgagggga ggacggtctg aatagcattt atatttgcaa
     3241 gcggtgttat atcaatttgt tatatgaatc tatttat
//
LOCUS       DBNGZA010000446         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_90981, whole genome shotgun sequence.
ACCESSION   DBNGZA010000446 DBNGZA010000000
VERSION     DBNGZA010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_90981"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="AC16FU_09560"
     CDS             <1..509
                     /locus_tag="AC16FU_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994655.1"
                     /translation="LSDGSHSYRHWNSAAPPDPEHWGITISGFGKNFDPKAVHPYEPK
                     EAQGDILTQMIRLKDDEDRREQFSLTGPTQVRIYALGEGDEDEMYDYGWIVNKRTGRT
                     VWEMTWRNTEPAGGATKNRKYDDIVWLDKGEYEVHFVTDGSHAFGDWNAARPADPTHW
                     GITITRVK"
     gene            1613..2836
                     /locus_tag="AC16FU_09565"
     CDS             1613..2836
                     /locus_tag="AC16FU_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HIR2994656.1"
                     /translation="MPSAFRFFLRLSSAGTIFLVLFFSFSAVFGQAPVLLYQKNGAAA
                     FDFLGNSVAGAGDVNGDGRADFIVGAPFADPGGITNAGSAYVYSGFTGALLFQKNGAA
                     VGDLLGSSVAGAGYVNGDALADFIIGAPSADPGGVSDAGSAYVFSGATGVLLYTNDGA
                     VAGDDLGFSVAGAGDMNGDGFEDFIIGAPRADPGGLSRAGSAYVYSGFTGGLLFQSNG
                     AVAGYLLGASVAGAGDVNGDLRSDIIIGAPSDDPGGPLGAGTALVISGLSSLTLAVIN
                     GTVGGDALGTSVAGTGDVDGDGKADFIVGARGASPGGMVAAGSAYVYSLPCLASGDVA
                     GGPNNCPDGAIDIADIVTVIGNVVVGTPFCGPAEAADVAGDPQGCPDTVVDIVDIVSI
                     INNVVFGTPFCQACL"
     gene            complement(2950..>3283)
                     /locus_tag="AC16FU_09570"
     CDS             complement(2950..>3283)
                     /locus_tag="AC16FU_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR2994657.1"
                     /translation="KRAPDLIVDGEMQVDSAVDPEVSAEHFPFSAIQGDANVLIFPEL
                     FSGNIAYKMMQRLGGAEVIGPILMGMRKPVHVLQQASDVNDITNITALAVVDAQSIST
                     RIGKASAP"
BASE COUNT          675 a          868 c         1020 g          720 t
ORIGIN      
        1 atctctccga cggctcccac tcctaccggc actggaattc agccgctccg cccgatcccg
       61 aacactgggg catcaccatt tccggcttcg ggaaaaattt cgatccaaag gccgtccacc
      121 cctacgagcc caaagaggcc cagggtgaca ttctgacgca gatgatccgg ctgaaagatg
      181 acgaggaccg ccgcgaacaa ttttccctca ccgggccaac ccaggtgcgc atttacgctc
      241 tcggcgaggg ggatgaagac gagatgtacg actacggctg gatcgtcaac aaacgcaccg
      301 ggcggacggt ctgggagatg acctggcgca acacagagcc cgccggcggc gccacgaaaa
      361 accgcaagta cgacgacatc gtctggctgg acaaggggga gtacgaagtc cacttcgtca
      421 ccgatggctc ccacgctttc ggcgactgga acgcggcgag accggctgac cccacccact
      481 gggggattac gattacgcga gtaaaataaa agtgtcgaag tgtcgaggtg tcgcggttcg
      541 acaagctcac cgccctgagc gaagtcgaag gggggtgtcg aggtaaagtt cataggttcc
      601 gggaaggggg cggcacgtaa ttttttttag cttttgccct gttgaaagag aagaagttcc
      661 atggagcaac acggcgcatt ttcggattgt gattgcttgt aatcatttgt tgtcctgaag
      721 gatagtggag ttccgagaag ggggcgggga atagagcaga gatcagggat ctgggattgg
      781 agatggggga tcagtagatt cacagcagat gaagcagagg gagccgaaga aaaggcagaa
      841 cggagtccgc atgaggcgga tgagaactga gtattgaggg caagacaaaa agacatatac
      901 agcacctcgg cctctcagga gtaaggagtc ccggattttg gcccctggct actggccact
      961 ggctcctgat gtgctcctac tatttcgcac attgtccgtt ttacccccgt cgtgacccca
     1021 aatttctgcg aggctggccg gtacggctct atcgagaagc ctcgcctatg ggaaaggggg
     1081 gggcgtttta cccctatgat gatcccaaat tcgacggggg aaggggtgcc tgtgcgcccc
     1141 cgcgacgact ccaaaattca gcccgcccat cgcggaggcg gcactctcag tgccgtccct
     1201 tttggcggga ctgaagtccc gcctacagaa ggaacctttt taccctcgcg ctaactccga
     1261 gtaagccatt gattaataat gagtgacagt gatcgaggat tgctttttgg gagaaggtaa
     1321 gcgctcccgg tatgggcact tttttatttc ggacgaaaaa agtccttgac aagaatagat
     1381 ggtcggctat tcttccattg tcgggggagc cgaccaccat aaggaggtgc gggcaagttt
     1441 tagttccgga ttagttaaag accggaatta cattttccgt ccgagcaacc agtgtaactt
     1501 catccgtcac caggtgcgtg taccttcact ttctgtagaa gtacaaaacc agtgatttaa
     1561 ccatcgcgaa cgactcgttc tcgaaatgag ccgtagcgag tgcggaggtc gtatgccgag
     1621 tgctttccgt ttttttctcc ggctcagttc agcagggaca attttcctcg ttctgttttt
     1681 ttctttttca gccgttttcg gccaggcgcc ggtgctgctc taccagaaaa atggggcggc
     1741 ggcctttgac tttctgggca attcagttgc cggggccggg gatgtcaatg gcgacgggcg
     1801 ggccgatttc atcgtcggcg ctcctttcgc cgacccgggc gggattacga atgccggctc
     1861 ggcttatgtc tattccggct tcacgggtgc cctgcttttc cagaaaaatg gcgcggcagt
     1921 aggtgacctt ctgggctcct ccgtggccgg ggccgggtat gtgaatggag acgcactcgc
     1981 cgatttcatc atcggcgctc ctagtgccga cccgggcggg gttagtgatg ctggctcggc
     2041 ttatgtcttc tccggagcca ccggcgtgct tctctacacg aatgatgggg cggtagcagg
     2101 tgacgatctg ggcttctccg ttgccggggc cggggatatg aacggggatg gatttgagga
     2161 tttcatcatc ggcgctcctc gtgccgaccc gggcgggctg agcagagcgg gatcggccta
     2221 tgtctactcc gggttcaccg gcgggcttct gttccagagt aatggggcgg tagcaggtta
     2281 cctgctgggc gcttcagttg ccggagccgg agatgtgaat ggagacttga ggtctgacat
     2341 aatcatcggt gctccctctg acgatccggg tggaccctta ggcgcgggta cagctttggt
     2401 catttccggc ctttcgtctt taacccttgc cgtaataaac ggcaccgtgg gtggtgacgc
     2461 tctgggcacc tccgtcgccg gaaccgggga tgtggacggg gatgggaaag ccgatttcat
     2521 cgtcggcgct cgtggtgcct ccccgggcgg aatggtcgct gccggttcgg cttatgtgta
     2581 cagtctgccg tgcctggcgt caggagatgt ggcaggtggc ccgaacaact gcccggatgg
     2641 tgcgatcgac attgcagaca tcgtcacggt gatcggcaac gtggtggtcg ggacgccctt
     2701 ctgcgggccg gcggaagcgg cggacgtggc gggcgatccg caggggtgtc cggacactgt
     2761 cgtggacatc gtggacatcg tttccataat caacaacgtg gtgttcggga cgccgttctg
     2821 ccaggcgtgc ctgtgaaaag cagggattag ggatcagaga tcagggatta gaagcaatcc
     2881 gacctacccc gaccctctcc tacccatacg ggtctttcga cagaagagga ctcggtacgc
     2941 aaaccttgat tagggagcgg aggctttccc gatacgggtc gatatcgact gcgcgtccac
     3001 aacggcaagg gcggtgatgt tcgtgatgtc gttcacatcc gacgcctgct ggaggacgtg
     3061 gaccggcttg cgcatcccca tcagaatcgg gccgatcacc tcggcgccgc ctaagcgctg
     3121 catcatctta taggcgatat tgccggagaa aagttcgggg aaaattaata cattcgcgtc
     3181 tccctgaatg gctgagaagg ggaaatgttc ggcggatact tccggatcga ctgcggaatc
     3241 gacctgcatt tcgccgtcaa cgattaaatc cggcgcacgc ttt
//
LOCUS       DBNGZA010000447         1721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1364223, whole genome shotgun sequence.
ACCESSION   DBNGZA010000447 DBNGZA010000000
VERSION     DBNGZA010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1364223"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC16FU_09575"
     CDS             <1..606
                     /locus_tag="AC16FU_09575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR2994658.1"
                     /translation="EGPPLPTEESQEPRPLSPYAVSKLAAEQYTRVFWELYRVPTVCL
                     RYFNVYGPLQSPGSAYAAVIPRFIHASLHDLRPQVFGDGLQSRDFTFVSDVVRANLLA
                     CAEDQAVGQTLNIACGQSHSLNELLNQLERISGKKVAPIYLDPRPGDIRHSQAAIKKA
                     QGILGFTAETSFAEGLEKTYRWFAEKEGRLRPREEHALSFA"
     gene            587..>1721
                     /locus_tag="AC16FU_09580"
     CDS             587..>1721
                     /locus_tag="AC16FU_09580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR2994659.1"
                     /translation="MPSVLRKRKPVSSQAGAVPGSPLAVTFVYPTDPRGVKVGGVETF
                     IRSFLELAPTEVEARLVGIDTAGDLACGTWQTVTVSDRPVLFFPAFFEKHQANRRHPV
                     PLSLRFALQLWCFRRRLAVDNSVLSVHRVELSWPFWGHPRLVLFIHGASKFLSLPSES
                     RLRWIRPFFASVEARALEKARRAVVVSQEGLDYYRNRFPGLNGKMRLISNWVDTQTFY
                     PRNKAALRRKFQIPLHVPVVAFVGRFEKVKDPELLLATFARIKAEFPKTLLAVCGAGE
                     EVRGQIPRELQVAVRLYGAVPHQTLAEILGCADLFLLTSFFEGMSRAVLESLACGVPV
                     VATDVGDIRKVVSNNLSGSVANSRSPEELAARGVAVLQNESITA"
BASE COUNT          303 a          527 c          519 g          372 t
ORIGIN      
        1 gagggcccgc cattgccaac ggaagagtct caggaacccc ggcccctgtc cccctatgcg
       61 gtgtccaaac tcgctgcgga acagtacacc cgcgttttct gggagttgta ccgggtgcct
      121 accgtctgcc tgcgttactt caacgtctac ggcccgcttc aatcccccgg ctcggcctat
      181 gcggcggtca tcccgcgttt catccacgct agcctgcatg acctgcggcc gcaggttttt
      241 ggcgacggcc ttcaatcgcg cgacttcact ttcgtctccg acgtggtgcg ggccaatctt
      301 ttggcctgtg ccgaagacca agcggtcggg cagacgctga acattgcctg cggacaaagc
      361 cattcgctga atgaactcct caaccagctc gagcgcatca gcggaaagaa ggttgcaccc
      421 atttacctcg atccgcgtcc cggcgacatc cggcactcgc aggcggcgat caaaaaagcg
      481 cagggaatct tggggttcac cgccgagact tcgttcgctg aagggttgga gaagacttac
      541 cgctggtttg cggagaaaga aggccgcttg aggccccggg aggagcatgc cctcagtttt
      601 gcgtaagcga aaaccggtct ccagtcaggc gggcgcggtg cccggctccc cgctcgcggt
      661 gaccttcgtc taccccaccg atccgcgggg ggtgaaggtc ggaggcgtcg aaacgtttat
      721 ccgcagtttt ctggagttgg cgccgactga ggttgaggcg cgtctggtcg ggattgatac
      781 cgcgggcgac ttagcgtgtg gtacgtggca aaccgtgaca gttagtgacc gcccggtcct
      841 gttttttccg gcattcttcg aaaagcacca ggccaaccgc cgccatcccg tcccgttatc
      901 tctccggttc gcactccaac tctggtgttt ccgccgccgc ctggctgtgg acaattcggt
      961 tctttcggtg caccgggtgg aactctcctg gcccttctgg ggacacccgc gtctggtttt
     1021 gttcattcat ggtgcgtcga aatttctatc cctgcccagc gaaagccgcc tgcgctggat
     1081 ccggccgttc ttcgcgtctg tggaagcccg ggcgcttgaa aaagcacgcc gggcggtggt
     1141 cgtgagccag gaagggctcg actactaccg aaaccggttc cccggcctga acgggaaaat
     1201 gcgtttgata tcgaactggg tggataccca aaccttttac ccccggaaca aagccgccct
     1261 ccggaggaaa ttccagattc ccctgcacgt tccggtcgtg gctttcgtgg gccggtttga
     1321 aaaagtcaaa gacccggagt tgcttttggc cacttttgcc cgcatcaagg cggaattccc
     1381 gaaaaccctg ctggccgttt gcggggccgg agaggaagtg cgcgggcaga ttccccggga
     1441 actacaagtt gccgtgcgct tgtacggggc ggttccgcac cagaccctgg cggagatatt
     1501 aggatgcgcg gacttgttcc ttttgacctc ttttttcgaa ggaatgtccc gcgccgtcct
     1561 ggagagtctg gcctgcgggg tgcccgtggt tgccaccgac gtcggcgaca ttcgcaaagt
     1621 agtgtccaat aatctttccg gctcggtggc gaactcccgc tctccggagg agttggccgc
     1681 gagaggggtc gcggttttgc agaacgaatc tatcaccgcc a
//
LOCUS       DBNGZA010000448        10882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2103274, whole genome shotgun sequence.
ACCESSION   DBNGZA010000448 DBNGZA010000000
VERSION     DBNGZA010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2103274"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16FU_09585"
     CDS             <1..519
                     /locus_tag="AC16FU_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994660.1"
                     /translation="SQLIGIAQGGYRPSLFASTSYQFQAQSNRFVPDGRDWIHSWNSA
                     LLIQIPIFDGFKTPAEVQQAKIDYRESQFRKKQLEDQVALEVRQALGNLNEGRERILA
                     QEKAVDLGRTGLSIAEVRYQNGVGTQLELIDSQVALTTAQTNQVQAFHDFLVAEAQLA
                     RAAGTELVHGEE"
     gene            562..1590
                     /locus_tag="AC16FU_09590"
     CDS             562..1590
                     /locus_tag="AC16FU_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994661.1"
                     /translation="MAATVTGLLFLFASAGCGEKGNAEKDAVPAEKVPVELASVLPRD
                     LHLTLTCSGSLEGFEQATLYGKIPEVVTAIKVKEGQRVTKGQLLIELEKAGPHSNYLQ
                     SQAIYLNAEKNYQKLENLYQQKAVSEQARDQAKTNYEVALANFNGARDLVEIKSPIGG
                     QVTEIDLVEGQQVPVGQVIAVVAKTAPLRLKFAVSYAEVKLLKPGQSVRVTSELQPGK
                     WVSGSIAEVSGSADPVTRTFQVEALLANPEGLFKPGMFAQGEAAVGTLKGALAIPKEA
                     VVLREGRPYVFAVNSDVAKLVAVETGAEAGGLVEIRSGLKAGDQVVTAGQSNLFDGLP
                     VRRAETAE"
     gene            1640..4747
                     /locus_tag="AC16FU_09595"
     CDS             1640..4747
                     /locus_tag="AC16FU_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR2994662.1"
                     /translation="MYLSNLSIRRPVFATMLIVALLVLGVTSYFQLNVDLFPEVDFPV
                     VAITTAYPGAGPEAVEAEVTKKIEDAVNPVAGIKHIASTSQEGFSTIIAEFTLETRIQ
                     DAAQEVREKVAAIRAFLPKEIEEPVIARFDPASQPIISLAISGERSLRDLTTYTKNKI
                     KKRLESIPGVGSVELVGGAEREIQVALAKSKLVAHDLPVEKVVSSIASANIEIPGGKV
                     GKGQREALLRTLGRFDPAGGAVPALQKVIVSTRQGRYVRLGEIAAVADSVKEPTSLSS
                     LNGREAVGLDITRQSGANTVAVASAVKAELARLEKELPPEMKIILAVDRSVFIHDAID
                     DILVNIYYGGALAILVIFLFLANWRSTLISALAIPTSIIATFTLMRLLHFTVNFMSLL
                     GLSLAVGLLIDDAIVVIENIYRHLDRGESAREAAKNATAEIGLAVMATTFSIVVVFLP
                     VAFMSGIVGRFFYQFGMTVAAAVLVSLFVAFTLTPMLSSRLLRPETKAERRGFFWKIP
                     LGWNAAFQKLNGVYRRVLAWALGHRKTTVAIALGTFVASFILVPFMGVEFIPQSDRGE
                     FVVNFETPTGSSLAHTAAVAEKIEQRIRRHPEVTTVLTTIGAGQNEVHQGRINVKLVE
                     RSKRKAGVFTFMNTLRWELAGLPGIKLAIGTEGMAGRTAKQIELSVRGDDPVLLRRLA
                     GRVEEIVRRTPGTVDVDNSEKAGKPELRVAINRDKASDLGLSPRSIAATLRTLVDGEV
                     ATKYQEGDEQYDVRVRLAEGDRSNPAAIAQLAVASTKEIEGKKDFLVPLNQVATLVEE
                     TGPTTINRYDRQREILITANVSGHFFGNVIREIQQQTGEISPPPGFHIGATGQAEAQK
                     ESFGYIFQALFLAVVFVYLLLASQFESFVDPFAIMLSLPLSLVGAFLGLFLTGSSVSI
                     MSLIGVVMLMGLVTKNAILLVDFTKQLRRKGVERTEALLTAGPIRLRPILMTTFAMVF
                     GMAPLALGFGPGAEMRAPMARAVIGGVISSTLLTLVVVPVVYTMLDDLVHRKRKTAPL
                     PVTES"
     gene            4902..6539
                     /gene="bshC"
                     /locus_tag="AC16FU_09600"
     CDS             4902..6539
                     /gene="bshC"
                     /locus_tag="AC16FU_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HIR2994663.1"
                     /translation="MQKTIDFRQIAATTRLFADFLYAPDKVQEFYNGSSADLESFRRV
                     GEFLRSRNYPRSAIAGILKEQNQAFGAGARTLANLDLFQTPDSRAVFAGQQVGLFGGP
                     LFTLYKMITIIKWAEKLGAQLNQKCIPFFWLATDDHDFAEIDHIAVLDHKTQLVQIRY
                     APGTPHNGSSMARVVLEATLEATIDELSRHLPETEFKSEVIAALRDCYQAGQPLHLAF
                     ARWVFRLIGDRGLVLVNPADPRLKALAQPVFQREIESTVQTSRLTQQTSSRLIQKQYH
                     AQVHKAETIVNLFYHDGNRAGISRSGDRFAIDGEKRSFSSAELVREVAVHPERFSPNV
                     FLRPVVQSAVFPTVAVVTGPSEVAYFAQIKGLFEHHGVPMPVLCPRVSLTVVEKRIAT
                     LVEKYQIDLGAFFRDPEGTTNRVLHQAIADPLEPKIDRTRTDLQEKLSTLEPDLVTLD
                     PTLKATFGQIKARVDLELKNLEKKVFAAQKKKNETIVEQLQRLRNHLYPGNKLQERQF
                     NIVYFLVKYGFGFSNHLFDALDIDRRDHQLVEVGGEG"
     gene            6536..7657
                     /gene="bshA"
                     /locus_tag="AC16FU_09605"
     CDS             6536..7657
                     /gene="bshA"
                     /locus_tag="AC16FU_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR2994664.1"
                     /translation="MKIGIACYPVPGGSGAVASELGLALARRGHEVHFLSYALPFRLA
                     AYTENFFFHEVEVSDYPLFKYPPYTLTLASKMIEVSRQWGLDVLHVHYAIPHASAAFL
                     AKQVLQSRLPVVITTLHGTDITLVGTNKSFYDVTKFSIECSDGITAVSDFLRQKTVAE
                     FDIKKEIRVIPNFVDTERFSDKNPACQRARFAPNGEKILMHLSNFRAVKRIGDVIEVF
                     RRVRKEIPAKLVLIGEGPETGQALALTRKFKLQNDTIFLGQQEFVENLLCLGDLFLLP
                     SETESFGLAALEALSCGVPVLATRTGGLAELVRDGENGFLFPVGESGKMAEQALELFR
                     DGDQLAWMKRQARQSAVERFDAKKMVPLYEAYYREVAGA"
     gene            7783..8493
                     /gene="bshB1"
                     /locus_tag="AC16FU_09610"
     CDS             7783..8493
                     /gene="bshB1"
                     /locus_tag="AC16FU_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR2994665.1"
                     /translation="MNLDCLVFAAHRDDIEITCGGTVIKMTDLGYKVGICDLTQGEMG
                     TKGTEVDRAREADAAAKVMGLSVRENLKLPDAGVQNTRENQLKVVEILRRYKPHLLIL
                     PYWEQRHPDHAVTPRLVYDACFLSGLKKLGAPGEPHRPFKILYSTSFFEIKHSFIVDI
                     TNQFSRKLEAVRCYNSQFDDTPESKEIFPPARNIFEFMETKARHYGYLVGKKYGEAFL
                     TREYVEVEDPMKLLVKSI"
     gene            8572..9279
                     /locus_tag="AC16FU_09615"
     CDS             8572..9279
                     /locus_tag="AC16FU_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HIR2994666.1"
                     /translation="MNRGNNNSTSFLNELDAIIAQRHMLSHPFYQAWSAGELSLETLR
                     HYARQYYHHVITFPRYLSNVHANCEDGQTRQVILENLIEEERGPENHPELWLRFGEAL
                     GDTREQVQSAIPLPETEAAVGTFLRLTREGSVAEGLAALYAYESQIPEVSKTKTEGLK
                     KFYGVTNLRGTAYFTVHEEADVRHAAQERELLASLSNTDITRRKARTSVRLALEAAWQ
                     LLDGIARTAPQPIVPCR"
     gene            9474..10031
                     /locus_tag="AC16FU_09620"
     CDS             9474..10031
                     /locus_tag="AC16FU_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994667.1"
                     /translation="MTLEEVLVFVLRRLHDQNIPYMLTGAVAVNYYGRPRLTHDIDLV
                     VEVREKQIPALVTAFAQDFYASQEGITDAIVHRSSFNFIHHASGLKIDFWLVQGTPFD
                     EERFARRQQVVLFDERVWVTTAEDLILIKLLWLKESGSQKHRDDIQGIVAVQKDRLSN
                     QYVERWTVQQSTRALWGDVIAHLES"
     gene            10093..10189
                     /locus_tag="AC16FU_09625"
     tRNA            10093..10189
                     /locus_tag="AC16FU_09625"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10127..10129,aa:Sec,seq:tca)
     gene            10199..>10882
                     /gene="selA"
                     /locus_tag="AC16FU_09630"
     CDS             10199..>10882
                     /gene="selA"
                     /locus_tag="AC16FU_09630"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR2994668.1"
                     /translation="MADTKKTAPGKSSALRQLPSVEKLLEAPQVRAFADQLPQPVVAE
                     VIRTVVDECRKKIDSGQGKVDLPALLATLQTRLVAKTRQALEQVVNGTGVILHTNLGR
                     APLGEEVIAAMAQAARQYVNLEYRLSDGTRGSRGAYAEELLCQLFGCEAAAVVNNNAG
                     ALFLALSAMAKGKEVVISRGELVQIGGGFRIPEILAESGAVLREVGTTNQTELADYRR
                     ALGPQTGLIL"
BASE COUNT         2310 a         3198 c         3156 g         2218 t
ORIGIN      
        1 agccagctga tcggcatcgc ccagggcggc taccgcccgt cgcttttcgc ctccacctcc
       61 taccagtttc aggcgcaatc gaaccggttc gtcccggatg gccgggactg gatccactcc
      121 tggaacagcg cgcttttgat ccagatcccc attttcgacg gatttaagac tccggccgaa
      181 gtgcaacagg ccaagattga ctaccgcgag tcgcagttcc gcaaaaagca gctcgaagat
      241 caggtggcct tggaggtgcg gcaggcgctg gggaatttga acgaaggccg ggaacggatt
      301 ttagcccagg agaaagcggt tgacctgggg cgtaccggtt tgtcgatcgc cgaagtgcgc
      361 taccagaacg gggtggggac gcaattggaa ctgatcgatt cgcaggtggc cttgaccacc
      421 gcccagacca atcaggtaca ggccttccac gattttctgg tggccgaagc gcaactcgcc
      481 agggccgccg gcacggaact ggttcacggg gaagagtaag gaatgaagaa gggtagcctt
      541 atgaaaaaaa gtctaaagcc aatggcagcg acggtcaccg ggcttctttt cctgttcgcc
      601 tcagccgggt gtggcgaaaa aggcaatgcg gaaaaagacg cggtgcccgc cgaaaaagtg
      661 ccggtagaac tggccagtgt cttgccccgc gatctgcatt tgactttgac ctgctctggc
      721 agcctcgagg ggttcgaaca ggcgacgctg tacggcaaaa ttcccgaagt ggtgacggca
      781 attaaggtta aagaagggca gcgggttacc aaggggcagc tgctcattga actggaaaaa
      841 gccgggcccc actccaacta cctccagtcc caggcgattt atctgaacgc ggagaagaac
      901 tatcagaagc tggagaacct gtatcagcag aaagcggtct ccgagcaggc ccgcgaccaa
      961 gccaaaacca actacgaggt ggcgttggcc aacttcaatg gcgcgcggga cctggtggaa
     1021 atcaaatcgc ccatcggggg tcaggtcacc gaaatcgatc tggtggaagg ccagcaggtg
     1081 ccggtggggc aggtcattgc ggtggtggcc aagaccgcac ccctgcggct caagtttgcc
     1141 gtcagttatg cagaagtgaa gctcctcaaa ccgggccaga gcgtgcgggt cacctccgaa
     1201 ctccagcccg ggaagtgggt ctccggcagc attgccgaag tctccggctc agccgatccg
     1261 gtcacgcgca ccttccaggt cgaagcactc ctggcgaatc ccgaagggct gttcaaaccg
     1321 gggatgttcg cccagggcga agcggcggtg ggcaccttga aaggcgcact cgccattccc
     1381 aaggaagcgg tcgtgctgcg ggagggaagg ccctacgttt ttgcagtaaa ctccgatgtt
     1441 gccaaactgg tggcggttga aaccggcgcc gaagcgggcg gtttggtcga aattcgctcc
     1501 ggcctcaaag ccggcgacca ggtcgtcacg gcggggcaga gtaacctttt cgacggctta
     1561 ccggtgcgcc gggcggaaac cgcagagtaa ctcgcaagga tgattaattc aggaattttt
     1621 cccaaaagcg ccgtgaacca tgtatctctc taacctttcc atccgccggc cggtcttcgc
     1681 cacgatgctg attgtggccc ttctggttct gggcgttacc tcctacttcc agctcaatgt
     1741 cgacctcttc cccgaagttg atttcccggt ggtggcgatc accacggcct atcccggcgc
     1801 cggaccggag gcggtggagg ccgaggtgac caagaagatc gaagatgccg tcaatccggt
     1861 cgccgggatc aagcacattg cttccacttc tcaggagggg ttttccacca tcattgccga
     1921 attcacgctc gaaacccgga tccaggacgc ggcgcaggaa gtccgggaga aggtggccgc
     1981 catccgggcg ttcctcccca aagaaatcga agagccggtg atcgcccgtt ttgaccccgc
     2041 cagccagccc atcatctcgc tcgcgatctc aggcgagcgg tccctccgcg acctgaccac
     2101 ctataccaag aacaaaataa aaaagcggct cgaatcaatt cccggggtcg ggtcggtgga
     2161 actggtgggc ggagccgagc gggaaataca ggtggcgttg gccaagagca aattagtggc
     2221 ccacgacctg ccggtggaaa aagtggtcag ttccattgca tcggccaaca ttgaaattcc
     2281 cggcgggaag gtgggcaagg gccagcggga ggcgctcttg cgcaccctgg gccgttttga
     2341 tccggcgggg ggggcggtgc cggcgctgca gaaggtcatc gtctccaccc gtcagggcag
     2401 gtacgtccgg ctgggggaga tcgccgcggt agccgacagc gtgaaggaac cgacttccct
     2461 ctcgagtctc aacggccggg aggcagtcgg cctcgatatt acccggcagt cgggcgccaa
     2521 tacggtggcg gtggccagcg cggttaaagc cgaactggcc aggttggaaa aggaactccc
     2581 ccctgaaatg aaaatcattt tggcggtgga ccgctccgtc tttatccacg atgccatcga
     2641 cgacattctg gtcaatatct actatggcgg ggcgctggcc atcctggtca ttttcctctt
     2701 tttggctaac tggcggagca cgctcatttc ggccctggcc atccccacgt cgattatcgc
     2761 caccttcacc ctgatgcggc tgctccattt tacggtgaat ttcatgagtt tgctcggcct
     2821 ctctctggcc gtggggcttt tgattgacga cgccattgtc gtgatcgaaa atatctaccg
     2881 gcacttagac cggggcgagt cggcccggga agcggccaaa aatgcgaccg ccgaaatcgg
     2941 cctggccgtc atggccacca ccttttccat tgtggtggtc ttcctgccgg tggcgttcat
     3001 gtcggggatc gtcggccgtt tcttctacca gttcggcatg acggtcgccg ctgcggtttt
     3061 agtctccctc ttcgtcgctt tcactctcac gccgatgctc tcctcgcgtc ttttgagacc
     3121 ggaaacaaag gcggagcgcc ggggattttt ctggaaaatt cctttgggct ggaatgccgc
     3181 gtttcagaaa ttaaacggcg tctaccgccg ggttttggcc tgggccctgg ggcatcgcaa
     3241 gacgacggtg gcgattgctt tggggacctt tgtggccagt ttcatcctgg tccccttcat
     3301 gggggtggag ttcattcccc agtccgatcg gggcgagttt gtggtcaact ttgaaacgcc
     3361 gaccgggtcg tccttagccc acaccgcggc ggtggccgaa aaaattgaac agcggattcg
     3421 ccggcacccc gaagtgacta ccgtgctcac gaccatcggc gccggccaga acgaagtcca
     3481 ccaggggcgg atcaacgtca agctggttga gcggagtaaa cggaaggcgg gtgtttttac
     3541 cttcatgaac accctccgct gggagctggc gggcctcccc ggaatcaaac tggccatcgg
     3601 caccgaggga atggcgggcc gtaccgccaa gcaaatcgaa ctttccgttc gcggcgacga
     3661 tccggttctt ttgcgccgcc tggcgggtcg ggtggaagag atcgtccgac gcacgccggg
     3721 gacggtcgat gtcgataaca gcgagaaggc cggcaaaccg gaactgcggg tggcgatcaa
     3781 ccgggacaaa gcgtcggact taggattgtc gccgcgctcg attgcggcca cgttgcggac
     3841 cttagtggat ggggaagtgg cgaccaagta ccaggaaggg gacgagcaat acgacgtgcg
     3901 ggtccgcctg gcggaaggag atcgctcgaa tccggccgcc attgcgcaac tcgccgtcgc
     3961 cagtaccaaa gagattgagg ggaaaaaaga ttttctggtg ccgctgaacc aggtggcgac
     4021 cctggtggaa gagaccggcc ccaccaccat caaccgctac gaccggcagc gtgaaatcct
     4081 cattaccgcc aacgtgtccg gccatttctt cggcaacgtg atccgcgaga tccagcaaca
     4141 gaccggagag atcagtcccc cgcccggctt ccacatcggc gccaccggcc aggccgaggc
     4201 gcaaaaagaa tcgttcggct acatctttca ggcgctcttt ctggcggtcg tcttcgtcta
     4261 cttgcttttg gcttcacaat tcgaaagttt cgtcgatcct ttcgccatca tgctctcgct
     4321 cccgctttcc ctggtggggg cgtttctggg ccttttcctc accggcagct cggtctcgat
     4381 tatgtcgctc atcggcgtcg tgatgctcat gggtctggtg accaaaaacg ccatcctctt
     4441 ggtcgatttc accaagcagc tccggcgcaa gggggtggag cgtaccgaag cgcttttgac
     4501 cgccgggccg atccggctgc ggccgattct catgaccacg ttcgcgatgg tgttcggcat
     4561 ggccccgctg gcgctggggt tcgggcccgg agcggaaatg cgggcgccga tggcgcgggc
     4621 cgtcatcggc ggggtaatct cttcgaccct cctcacctta gtcgtcgttc cggtcgttta
     4681 caccatgctg gatgacctgg ttcaccggaa gcgcaagacc gctccgcttc cggtgaccga
     4741 aagctgatct ctcttctcgc gcagttggga gcatccccaa aagtgctcaa tgcggtggcg
     4801 ccggttggtt tttgaagccg gcactacaga ttttccttta ccggaaatgt tattccccac
     4861 cccctgaata gcgtatattg tcaaagattt cgagggagtg aatgcagaaa accatcgatt
     4921 ttcgccagat tgcggcgacg accagactct tcgccgattt cctctacgcc ccggacaagg
     4981 tccaggagtt ctacaacgga agttccgctg accttgaaag tttccggcgg gtaggcgagt
     5041 ttctgcgttc gcgaaattac ccgcgttccg ctatcgccgg tattcttaag gaacaaaacc
     5101 aagccttcgg tgccggagcc aggactctgg ctaacctcga tcttttccaa acgcccgaca
     5161 gccgggcggt ctttgccggt cagcaggtgg gcctcttcgg cgggccgctc ttcacactct
     5221 ataagatgat cacgatcatc aagtgggcgg aaaagttagg cgctcaactg aaccagaagt
     5281 gtattccctt cttctggctg gccaccgacg accacgactt cgccgagatt gaccacatcg
     5341 ccgtcctcga tcacaagact cagttagtcc aaatccgcta cgcaccgggc acaccccata
     5401 atggctcgtc aatggcgcga gtggtgctcg aggcgaccct cgaggcgacc atcgatgaac
     5461 tttcccgaca cctacccgaa acagaattca aaagtgaagt gatcgctgcc ctgcgcgact
     5521 gctaccaagc gggtcagccg ctccacctcg cctttgcccg ttgggtcttt cgcttgattg
     5581 gtgaccgggg attggtcctg gtcaatccgg ccgatccccg attgaaggcg ctggcgcagc
     5641 cggtctttca gcgtgaaatc gaatcaaccg tccaaaccag tcggcttacc cagcaaacat
     5701 ccagccggct cattcaaaag cagtaccatg cgcaggtgca taaggccgag acaattgtca
     5761 atctcttcta ccatgacggc aaccgggccg gcatcagccg ctccggtgat cggttcgcca
     5821 tcgacgggga aaagcgctcc ttttcatccg ccgaattggt gcgggaagtg gcggtccacc
     5881 cggagcggtt cagcccgaac gttttcttgc ggcccgtggt gcagagtgcg gttttcccga
     5941 cggtggccgt ggtcaccggc ccgtcggagg tggcctactt cgcgcagatc aaggggctct
     6001 ttgaacacca cggtgtcccg atgccggtcc tctgtccccg agtctcgctg acagttgttg
     6061 agaagcgcat tgccaccctg gtagagaaat accagatcga tctgggagcc ttcttccgcg
     6121 atcctgaggg caccaccaac cgcgtcctcc accaggccat tgccgatccc ttagagccca
     6181 aaattgaccg gacgcgcacc gatttacagg agaaactctc aactctggaa cccgacctgg
     6241 tgacgctcga tccgacgctg aaagcgacct tcggtcaaat taaagcccga gtggacttgg
     6301 aactgaaaaa cttagagaag aaggtttttg ccgcgcagaa gaaaaagaat gaaaccatcg
     6361 ttgaacaact ccagcgattg cggaaccacc tctatcccgg aaacaaattg caggagcggc
     6421 agttcaatat cgtctacttc ttggtgaagt acggtttcgg tttcagcaac cacctctttg
     6481 acgcgctgga tatcgaccgg cgcgaccacc agctcgtcga agtcggaggc gagggatgaa
     6541 aatcggtatc gcctgttacc ccgtcccggg cggttccggc gcagtggcga gtgaattagg
     6601 cctggccttg gcccggcggg gacacgaggt tcactttctc agttatgcgc tgcccttccg
     6661 tctggctgcc tataccgaga acttcttctt tcatgaggta gaagtcagcg attacccgct
     6721 cttcaaatac ccgccctaca cgctcaccct ggcttccaag atgatcgagg tctcgcgcca
     6781 gtgggggttg gatgtcctcc acgtccacta cgccattccc cacgcttcag ccgcattcct
     6841 ggccaagcag gtgctccaga gccgcctgcc ggtggtcatc accaccctgc acggcaccga
     6901 cattacgctc gtcgggacaa acaagtcctt ctacgacgtc accaagtttt ccatcgagtg
     6961 ttccgacggc atcactgctg tctccgattt cctgcggcag aaaaccgtag ccgaattcga
     7021 catcaaaaaa gaaatccggg tgattcccaa cttcgtcgat actgagcgct tctcggataa
     7081 aaacccggcc tgccagcgcg cccgtttcgc ccccaacggg gaaaagattc tcatgcacct
     7141 ctcgaatttc cgagcggtca agcggattgg ggacgtaatc gaggtctttc gccgggtacg
     7201 gaaagaaatt ccagccaaat tggtgttgat cggcgaaggc cccgagacgg gccaggcgct
     7261 cgccctgacc cgaaaattca aactgcagaa cgacaccatc tttttgggcc agcaggaatt
     7321 tgtggaaaat ctcctgtgtt tgggcgacct gttccttctg cccagcgaaa ccgagagttt
     7381 cggactggcc gcgctggaag ccctgagctg cggcgtgccg gtgctggcga cccggaccgg
     7441 cgggttggcg gaactcgtta gagacggtga aaacgggttc ctcttcccgg tgggagagag
     7501 cgggaagatg gctgagcagg cgctggagct gttccgggat ggcgaccaac tggcctggat
     7561 gaaaagacag gcgcgccaga gcgcggtcga gcgctttgac gccaagaaaa tggtacccct
     7621 gtacgaagcc tactaccggg aagtggcggg agcataagat cagtttttta cggctggccc
     7681 aactccgcaa cccacttgca aaattccaca aatcaagcat cttaccccgt ttcgtggagg
     7741 tgctcgttca ggacctgtga cgaaaaatag tgtggagatg gaatgaatct tgactgtcta
     7801 gtctttgccg ctcaccggga cgacatcgaa atcacttgcg gtggcacggt cattaaaatg
     7861 actgacctgg ggtacaaagt cggcatctgc gacctgaccc agggcgagat gggcaccaaa
     7921 gggactgaag tcgaccgtgc ccgcgaagcc gatgccgccg ctaaagtcat ggggctttcg
     7981 gtccgcgaaa atctgaaact ccccgatgcc ggcgtccaga acacccgcga gaatcaattg
     8041 aaagtggtgg agatactccg ccggtataaa ccccatttgc tcatcctccc ctactgggag
     8101 cagcgccacc ccgaccacgc cgtcacaccc cgcttggtct acgatgcctg cttcctctcc
     8161 ggtctgaaga aattgggagc tccgggcgag ccccaccggc ccttcaaaat cctctacagc
     8221 acctcgtttt tcgaaatcaa gcactccttc atcgtcgata ttaccaacca gttttcgcgg
     8281 aaattagagg cggtgcgctg ctacaactcc cagttcgacg acactcctga atcgaaagaa
     8341 atctttcccc ccgcccgcaa catctttgag ttcatggaaa ccaaggcccg ccactacggc
     8401 tacctcgtcg gcaaaaagta cggcgaggcg ttcctgaccc gggagtacgt ggaggtcgaa
     8461 gatccgatga agttgctagt gaagtcaatt taagatttcc gtttttccag cccattgtgt
     8521 atactccgga gtaattcatc ggtccgaaag gtgataacga aaggagtcac agtgaatcgg
     8581 ggaaataata attcgacctc atttctcaat gaactggatg ccattatcgc ccaaaggcat
     8641 atgctttccc accccttcta ccaggcctgg agcgcggggg aactctcgct ggaaacgttg
     8701 cgccactacg cccggcagta ctaccaccat gtcataacct ttccccgcta cctctccaac
     8761 gtccacgcca actgcgagga cggtcagact cgccaggtga tcctggagaa tttgatcgaa
     8821 gaggaacgcg gcccggagaa tcaccccgaa ctctggctgc gcttcggcga ggcccttggc
     8881 gacacccggg aacaagtcca atctgcaatt cccttgccgg aaacagaggc ggcggtcggg
     8941 acttttctcc ggctcacccg cgagggctcc gtggcggaag ggctggccgc gctctacgcc
     9001 tacgagtcgc aaatccctga agtttccaaa accaagactg agggattaaa gaaattctac
     9061 ggcgtcacta acctccgggg tactgcctac ttcacggttc acgaggaagc cgatgtgcgc
     9121 cacgccgcgc aggaacggga gttgctcgcc tcactttcca acacggatat aacccggcga
     9181 aaagcacgca ccagcgtccg actggctctg gaagccgcct ggcagcttct ggatggaatc
     9241 gctcgaaccg cacctcagcc gattgtgcct tgccggtgag tacccgtgcg accgtcggag
     9301 gcagaagtca aatatctcga agtactccgg gggttttccg gtgaagagcg ggtgcgtatt
     9361 ggttttgagc tctaccgctt agcccgccgg attgtagaag cttcggtacg agcccagtat
     9421 ccggacgcgt ccgatgaaga ggttgagcgt cacatccgtc agcggttggc cgcatgaccc
     9481 tggaggaagt gctcgttttt gtcctacgcc gtctgcacga ccaaaatata ccctacatgc
     9541 tcaccggtgc ggtggcggtg aactactatg gtcggccgcg cttgacccac gacattgatc
     9601 tggtggtaga agttagagaa aagcaaatcc cggccctcgt taccgctttc gcccaagatt
     9661 tttatgccag ccaggaaggg attaccgatg ccatagtgca ccggtcatca ttcaacttca
     9721 ttcaccatgc cagtggtttg aaaatagatt tctggctggt gcaagggact ccctttgacg
     9781 aagagcgttt tgcgcgccgc cagcaggtag tgctttttga tgaaagggtt tgggtgacca
     9841 ctgctgaaga cctcatactc attaagctcc tgtggttgaa agagtcagga tcccagaagc
     9901 accgcgacga catccaagga atcgtagcag tacagaaaga tcgcttgagc aaccagtacg
     9961 ttgagcgctg gactgttcag cagagcaccc gggcactctg gggcgatgtg attgcccatt
    10021 tagagagcta atcgcccaaa gtcgatttcg cctccgcttg ccccacggaa gcgggcgtgg
    10081 tagtttgccc ttggaagcgg atcggatctg gtggtccgag cggacttcaa atccgtatgc
    10141 ggctgcgttc gcgtggtcgt ggtaggttcg attcctaccc cttccgccac atttgattat
    10201 ggctgacaca aagaaaaccg ccccgggcaa aagctcagca ctgcggcaat tgcccagcgt
    10261 ggaaaaactt ttggaagcac cgcaggtgcg ggctttcgcc gaccaattac ctcagccagt
    10321 ggtggctgag gtgatccgca cggttgttga tgaatgccga aaaaaaattg attcgggcca
    10381 gggaaaagtt gatttgccag cactcctggc tacgctccag acgcggctgg tggcgaaaac
    10441 gcgccaggcc ctggaacagg tcgtcaacgg caccggggtg attttgcata cgaatttagg
    10501 acgggcacct ctgggtgaag aagtcatagc ggcgatggcg caagccgctc gccagtacgt
    10561 caatttggaa taccggctct ccgacggcac ccgcggcagc cggggtgcct atgccgagga
    10621 actcctctgt caactcttcg gctgcgaagc ggcggcagtg gtcaacaata acgccggcgc
    10681 gttgtttctg gcgctctccg ccatggcgaa gggaaaagaa gttgtgatct cccgcggcga
    10741 gttagtgcag atcggcggcg gcttccgcat tccggaaatt ctggccgaaa gcggagcggt
    10801 tttgcgcgag gtcggcacca ccaatcagac cgaactggcc gattaccgcc gggcgctggg
    10861 gccgcagacc ggcctcattt tg
//
LOCUS       DBNGZA010000449         2677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2951605, whole genome shotgun sequence.
ACCESSION   DBNGZA010000449 DBNGZA010000000
VERSION     DBNGZA010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2951605"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1736
                     /locus_tag="AC16FU_09635"
     CDS             216..1736
                     /locus_tag="AC16FU_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR2994669.1"
                     /translation="MKLLILFLLVLFLSAQSGNASPIVLNEILANEPGSATSLEWIEL
                     FNPGSTPATLDSMVLVVGPDTTRIPDGILLLPISFTVIARKPLSDPPGSASFEQHWGN
                     NSGVWGDTPEENFPLIQGKFSLANGADTVRLYSGSQLIDVFGWRHDAGDGISWERVLP
                     ADTLWLPATNPPGNTPGRINTQSPQPNDLTPLLFPPSLLASFPDSSHFLVSCEVKNVG
                     TATSLPNRVVLYDDRDFDSILVFTESLSTFSVPPLGIGMSQTFSDTLSLSTGPHRLWC
                     VLDPDDRPENNRASMDYVVGDFLPGVVLNEFLPDPTSAVGTEWIELVNRSAGPVNLKD
                     WKIRDAAGDTLAFAESLFVSHSEFLIAAKDKSLFAAAYPQVTSRVYQPATWLTLNNSG
                     DRIMLIDARGFAVDSVSYANGWGGNVSWERVDVNRPASASDNWWRSVAPSGSTPGQVN
                     SLASGFAQGLTLDINPNPFSPDGDGFDDETEISFSVPFASSLTLKILTCAGARSER"
     gene            1709..1897
                     /locus_tag="AC16FU_09640"
     CDS             1709..1897
                     /locus_tag="AC16FU_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994670.1"
                     /translation="MRGRQIRTLVEQTPQASGRLTWDGKGEDGKRVRAGIYIVFAESV
                     GSQHFTHKTTVVVAKSRE"
     gene            1894..>2677
                     /locus_tag="AC16FU_09645"
     CDS             1894..>2677
                     /locus_tag="AC16FU_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994671.1"
                     /translation="MRRFFFLFSLCGILFSSEIARSAFELQPLSTRALALGLSRFAPT
                     PTCLFSQPALLTKTPRSFQADYFRLYDLAELSQGVVAANLPRGDFAFGLGVSSFGRSD
                     YYRENQVAFGVARRVNNFSLGAGAKFWQVALPAPYSSPTGIAADLGLTYDLSTLGRFG
                     SSLGNLSLVASDDHLYPPVEIALGFSSTIANQFFVTLSGQWDDETKTSLRFGQEYHVN
                     SNLTLRFGFLTDPARFTLGLGVGKGKLHIDYGFLSHPVFSGSH"
BASE COUNT          507 a          819 c          681 g          670 t
ORIGIN      
        1 gccaagttct tggccgattt caaccgcacc cgtgcacatg agaggacgga tttctttcgg
       61 gtggaggtcg aggggaggtt ttgaggggat tgaggactga cccctccctg gccctcccct
      121 caaaggggag ggaaaattcc ttgctctctt tctttttatt agagaatagt cttatctttc
      181 cctctcctgt aggagagggt agggtgaggt cataggtgaa attgctaata ctgtttcttc
      241 ttgtactctt tctttctgcc caaagcggga atgcctcgcc cattgtcctc aacgaaatcc
      301 tcgccaacga accgggctca gccacttccc tcgaatggat tgaactcttc aatcccggaa
      361 gcacccccgc cacgctcgac agcatggtgc tcgtcgtcgg gccggatacg acccgtattc
      421 ccgacgggat ccttcttttg cctatatcct tcaccgtcat tgcccgcaag ccgctctcgg
      481 acccgcccgg cagcgcctca ttcgaacagc actggggcaa caactccggc gtctggggcg
      541 acacaccgga agaaaatttt ccgctcatcc aggggaagtt ctctcttgcc aacggcgcgg
      601 acactgtgcg cctttattct ggttcccagc tgatcgacgt cttcggctgg cgtcacgacg
      661 ccggtgacgg catttcttgg gagcgcgttt tgcccgcgga tacactctgg cttccggcga
      721 ctaatccgcc cggaaacaca ccaggacgga tcaatactca gtcaccacag cccaatgatt
      781 tgacgccgct tctttttccg ccgtcgctgc tcgcgagttt tcctgacagt tctcattttc
      841 tcgtttcttg cgaggtcaaa aacgtcggca cggccacttc cctgcccaac cgcgttgttc
      901 tttatgatga tcgcgatttt gattcgattc tagtattcac ggaatcactg agcacttttt
      961 ccgttcctcc attaggtatt ggaatgagtc aaactttttc cgacacactc tcgctctcca
     1021 ccggcccgca ccgcctctgg tgcgtccttg atcccgatga ccgcccggag aacaatcggg
     1081 catcgatgga ctacgttgtc ggtgatttct tgccgggtgt ggtgctgaat gaatttctcc
     1141 ccgacccaac ttccgcagtg ggaacggaat ggattgaact ggtcaatcgc tcagcggggc
     1201 cagtcaatct caaagattgg aaaatccgtg acgccgcagg cgacacgctc gcatttgcgg
     1261 agagtttgtt cgtgtcccat agcgagtttc tgattgcggc gaaggacaaa tcgctttttg
     1321 ccgccgctta cccgcaggtc acttcacgag tctaccaacc cgccacctgg ctcaccctca
     1381 ataacagcgg cgaccgcatc atgctcatcg atgcgcgcgg ctttgcggtc gattcagtgt
     1441 cctatgcgaa cggctggggc gggaacgttt cctgggaacg agtcgatgtc aaccgtcctg
     1501 cctccgcgtc cgacaactgg tggcgctcag tggcgccgtc cggttccaca ccggggcagg
     1561 tcaattctct ggcgagcgga tttgctcaag ggctaacgct ggatattaac cccaatccct
     1621 tttcgccgga tggtgacggt ttcgacgatg aaacggaaat ctccttttcc gtgccgttcg
     1681 cttccagtct cacgctcaaa atcttgacgt gcgcgggcgc cagatccgaa cgttagtaga
     1741 gcaaacgcct caagcatcgg gacgcctcac ctgggacggc aagggagagg atggtaaacg
     1801 cgtgcgggca ggaatttaca tcgtttttgc cgagagcgtt ggttctcagc atttcaccca
     1861 caaaaccacc gtcgtggtgg ccaaaagccg cgaatgaggc gctttttctt tctgttcagt
     1921 ctttgtggaa ttcttttctc ttcggaaata gccagaagcg cctttgaact ccaaccgctg
     1981 agcactcgcg ctcttgccct cggtttatcc cgctttgcgc cgacgcccac ctgtctcttc
     2041 tctcagcccg cattactaac aaaaactcca cgctcgtttc aagccgatta cttccggctc
     2101 tatgaccttg ccgaattgag tcagggcgtc gtggcggcga atcttccaag aggggatttt
     2161 gcctttggtc taggggtgtc gagtttcggc cgctccgact actaccgtga aaatcaggtc
     2221 gcctttggcg tcgcccgccg ggtgaacaat ttctcgcttg gtgcgggagc caaattctgg
     2281 caggtggcgc tcccggctcc ttattcgtcg ccgacaggta tcgccgccga tctcggcctg
     2341 acctacgatc tttccactct aggccgcttc ggctcctctc tcggcaatct ctcgcttgtg
     2401 gcgtcagacg accacctcta cccgccggtc gaaattgcgc tcggcttttc gagcacgatc
     2461 gccaatcaat tcttcgtcac cctctccggc cagtgggatg acgaaaccaa aacctctctc
     2521 cgctttgggc aggagtatca tgtgaactcg aatctcactc tgcgcttcgg ctttctcacc
     2581 gaccccgccc gctttacctt aggacttggc gtgggcaaag gaaaactcca tattgactac
     2641 ggtttcctga gccatccggt cttttccgga agccata
//
LOCUS       DBNGZA010000450         2234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_302621, whole genome shotgun sequence.
ACCESSION   DBNGZA010000450 DBNGZA010000000
VERSION     DBNGZA010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_302621"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="AC16FU_09650"
     CDS             <1..944
                     /locus_tag="AC16FU_09650"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HIR2994672.1"
                     /translation="ALEGPFGDHFGHYSHAAPFPVFHVNTVTRRRNPIYVAAVVGRPP
                     QEDKYMGKAAQEMFLPLIRLMHPEIRDLWAYYEAGFHNLLVVSVEQRYGKEALKTGLG
                     LLGEGQLALTKCMILVDSGVDVKNFSAVLRQIDRNFDPAYDFLLLPGVPLDTLDFTSF
                     TMNLGSKMILDATTKPRGDGKKDLRFRDPRSVDERIQAWKKWEETLLVVQVADHGRAV
                     VENLVKQELGQVKIVAAVSPDVDLDNRESLLWGIFTRFDAARDICFTRSELRGACPIH
                     RGVLGIDATFKKGYPLPIEMDPQITEGVTRRWPEYRI"
     gene            923..2053
                     /gene="mqnE"
                     /locus_tag="AC16FU_09655"
     CDS             923..2053
                     /gene="mqnE"
                     /locus_tag="AC16FU_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR2994673.1"
                     /translation="MARVPDIAFRDRQLVPIWEKVKCGEPLSREEGLACLQTHDLLAL
                     GRMANFVKEEKSKNYVFFVLNRQINPTNLCVLSCKFCDFAAKPGEAHAYEMTLEEILN
                     KVTDELREVHIVGGLHPTWRFEHYLEMMRAIKTKCPTVQLKAFTAVEVDFFAKMSKLT
                     LEQTLRKLLDAGLDTLPGGGAEVFSPRVRKELFKQKIGEQRWFEVHRVAHRMGIRSNA
                     TLLYGHIETYEERVDHLLKLRAVQAETGGFLTFIPLAFQPGTTGIVQRQTSALEDLRT
                     LATSRLILENFPHIKSYWVMLGEETASVGLHFGADDLDGTIGEEKIAHLALASSPVGL
                     TRQALLEMIKEAGKIPVERDALYNILKVYNGTADSQNSLPQR"
     gene            2013..>2234
                     /locus_tag="AC16FU_09660"
     CDS             2013..>2234
                     /locus_tag="AC16FU_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HIR2994674.1"
                     /translation="MGLRIAKIPYLNAEPFYAGLSATEFSLLEIPPRRLGELARAGEI
                     EAGLMPVVDFFALEKHFVPLADLGIAVRGA"
BASE COUNT          481 a          618 c          663 g          472 t
ORIGIN      
        1 gggcgctgga ggggcctttt ggtgatcact tcgggcacta ctcgcacgcg gcaccctttc
       61 ccgtgtttca tgtcaatacg gtaacgcggc gcagaaaccc aatctacgtc gccgccgtgg
      121 tggggagacc gccccaggaa gacaagtata tgggcaaggc agcccaggaa atgttcctgc
      181 ctttaatccg tctgatgcac ccggagatac gtgacctatg ggcctattat gaagccggtt
      241 ttcacaacct gctcgtcgtc tcggtggagc agcgttacgg caaggaggcg ctcaagaccg
      301 gcctgggact tctgggcgaa ggacagctcg cgctcaccaa gtgcatgatt ctggtcgatt
      361 ccggagttga cgtgaaaaac ttctccgcgg tgctgcggca gatcgacaga aattttgatc
      421 cggcctacga cttcctgctt ctacccggcg tgcctttgga tacgctcgac tttaccagtt
      481 tcaccatgaa tttaggttct aaaatgattc ttgatgcgac caccaaaccg cgcggcgatg
      541 gcaagaaaga cctacggttc cgtgatccac gctcggtgga cgaacgcatc caggcgtgga
      601 agaagtggga agagacgctc ctcgtcgtcc aggtggctga ccatgggaga gcggtggtcg
      661 aaaatctggt caagcaggaa ctgggacagg taaagatcgt tgccgccgta agtcctgacg
      721 ttgatctcga taatcgggag tcgcttctct ggggcatctt cacccgattc gacgcagccc
      781 gcgacatttg cttcacccgc tcggagttgc gcggggcctg cccgatccac cggggcgttc
      841 tgggcattga cgccaccttc aaaaaaggtt atcctctgcc catcgaaatg gacccgcaga
      901 tcacagaagg ggtgacgcga cggtggcccg agtaccggat atagctttcc gcgaccgaca
      961 gctcgtcccc atctgggaaa aggtcaaatg cggagagccg ctctcgcggg aagaaggact
     1021 ggcttgtctc cagacccacg accttctggc tttaggccgg atggccaact tcgtcaaaga
     1081 agaaaaatcc aaaaattatg ttttctttgt tctgaaccgg cagatcaatc ccaccaatct
     1141 ctgcgtcctt tcctgcaaat tttgcgactt cgccgccaag ccgggtgaag cgcatgcgta
     1201 tgaaatgact ctggaggaaa tcttgaacaa ggtcaccgac gaactccgcg aggtgcacat
     1261 cgtcggcggg ctgcacccca cctggcgctt cgagcattat ttagaaatga tgcgggccat
     1321 caaaaccaaa tgtccgacgg tgcaactgaa agccttcacc gcagtggaag tcgatttttt
     1381 tgccaagatg agcaaactga cgctggagca gacgctccgg aaacttttgg acgctgggct
     1441 ggacaccctg cctggcggcg gggccgaagt cttctccccg cgcgtgcgga aggaactctt
     1501 caagcaaaaa atcggcgagc agcgctggtt cgaggtgcac cgggtagccc accgaatggg
     1561 tattcgctcc aacgccacgc ttttgtacgg ccacattgaa acttatgaag agcgggttga
     1621 tcacctcttg aaattgcggg cggtgcaggc ggagacgggc ggtttcctca cgttcattcc
     1681 gctggcattt cagccgggca ctaccggcat cgtgcagcgt cagacctcgg ctctggagga
     1741 cctgcggacg ctggccacct cgcggttgat cctggaaaac ttcccgcaca tcaagtcgta
     1801 ctgggtgatg ttgggggagg agacggcgtc ggtggggttg cacttcggtg cggacgattt
     1861 agacggcacc atcggcgagg agaagatcgc ccacctggcc ttagcgtcga gtccggtcgg
     1921 cttaacccgt caagcgcttc tggagatgat caaagaagcg gggaaaattc cggtggaacg
     1981 cgacgcgctc tacaacattt tgaaggtcta caatgggact gcggatagcc aaaattcctt
     2041 acctcaacgc tgagccgttt tacgccgggc tgagcgctac ggagttctcg cttctggaaa
     2101 tcccgccccg gcggctgggg gagttggcgc gtgcaggtga aatcgaggcc ggcttaatgc
     2161 cggtggtcga tttctttgcg ctggaaaaac attttgtgcc tttggccgac ctaggcattg
     2221 cggtccgagg agcg
//
LOCUS       DBNGZA010000451         4376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_836124, whole genome shotgun sequence.
ACCESSION   DBNGZA010000451 DBNGZA010000000
VERSION     DBNGZA010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_836124"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..403)
                     /locus_tag="AC16FU_09665"
     CDS             complement(212..403)
                     /locus_tag="AC16FU_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994675.1"
                     /translation="MKDDSVARQLEALLNESDKCAHAIQELIPKIGDYDLVRLLKKID
                     ADLADIRHNLAVTKRLAEK"
     gene            complement(414..632)
                     /locus_tag="AC16FU_09670"
     CDS             complement(414..632)
                     /locus_tag="AC16FU_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994676.1"
                     /translation="MEEIKGQQLGALLAEMLRETDKRAHGLHRVMHRVKDYDLQRRLE
                     KIEADLADIRHTFAVAKRLSEQCLGERA"
     gene            967..1395
                     /locus_tag="AC16FU_09675"
     CDS             967..1395
                     /locus_tag="AC16FU_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR2994677.1"
                     /translation="MAPKVKLFADYLASRGLKLTGERQKIVIEIFRTPRHFEPEELLI
                     DLRGRHLKASRATIYRTLELLMEAGLIRKVCLGSGRFSYEPVSEDRAHDHLICVRCGK
                     VVCFIDTEAEAAKKRISAEKGFELTGQCFQLFGYCPACRS"
     gene            1538..2632
                     /locus_tag="AC16FU_09680"
     CDS             1538..2632
                     /locus_tag="AC16FU_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994678.1"
                     /translation="MQRGILMACLCVSSGGLAAAQETSPYHFHGYGEMHFNRPKIGTM
                     SQGAGDEIDFHRMVFGFGYDFSEKIRLDFELDFEHAFTEPELEYAQVDFDLLPGLTLR
                     GGNLLIPVGPLNEFHEPPLFYSVERPYVQRSIIPTTWQENGAGVVGRVLDNRLAYRAY
                     AVSGLDGAKITSLDGLHDVSSKGAEAKANDFAGTGRVEFTPIRELTVAASGYFGGADQ
                     DEPGLGKVEVDIAEGDFHFQIQDIDLTGVFVRTKITGARQLSLAAGETIGKAMQGWYG
                     EAAVHTLKRLLPETSHDLVLFGRFEKFDTNHEVPAGFVQNLKADREIFTFGLAYYPID
                     RVTVKSDLELWKDGTDDTVSRFNLGLAFMY"
     gene            2651..3202
                     /locus_tag="AC16FU_09685"
     CDS             2651..3202
                     /locus_tag="AC16FU_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994679.1"
                     /translation="MKTSQTFASFLGLSLFLSSLAWAGEKKDIAQPFARLTVKFNGCA
                     SCAGCRSAMRQVAQTKSARHARIQIGDDRSLSATYPKATNIPMRELPAALGKSGLHQF
                     EVAEVTLETKGTVTEKDGQFFLILPETGQCLLLSGLANAEEVTGQELALRGKVRNWQS
                     PEEQLVLDVQSAQSIASAASERK"
     gene            3210..3488
                     /locus_tag="AC16FU_09690"
     CDS             3210..3488
                     /locus_tag="AC16FU_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR2994680.1"
                     /translation="MPSATGEAKIPAPVRKNLAEFTPGQVGVVSEIHGEVELTLRMME
                     IGLVPGVRVRYLRAAPLGDPIQVEVEGFLLSLRKSEAEAVILHSSELP"
     gene            3485..>4376
                     /locus_tag="AC16FU_09695"
     CDS             3485..>4376
                     /locus_tag="AC16FU_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR2994681.1"
                     /translation="MTAQLTEKNVFTVALVGNPNCGKTTLFNALTGLRQKVANYPGVT
                     VEKKTGLWEIEGTTVEALDLPGSYSLVPRSPDEKMVADVLSGRRPDLPAPDAVICVVD
                     VTNLSRHLYLVGQVIELGRPTVVALTMVDEAERIGLTVDTAKLSARLGVPVVAVIAAR
                     RVGLDQLALAVKKGVFSQWRSPERFWRLLPAFENRVKAYAPEAAATELETVACVAGLH
                     TPACTEKLEKVANQIRSELGVNEETVRRYVVEARYRWQREVTREVVEKTGDPRVDWTK
                     RLDKIFLHSILGPLVLLVRLT"
BASE COUNT          953 a         1200 c         1283 g          940 t
ORIGIN      
        1 ggtctaaccg gagatcctgg gccttgtaga cgacccccat tcccccttca ccgagtttgg
       61 ctgagatttt gtaatgggag atggtctggc cgatcatacg ttcgttttct tgattttacg
      121 gttcaggagc agcaccggcg gcggccgggg gactcaaaac caccggtggg aagagcagac
      181 ccgcagtggc ttcacccata cccgtgggag actacttttc cgccaggcgt ttggtcacgg
      241 ccaggttgtg gcgaatgtcg gccaggtcgg catcaatttt cttcaagagc cgcaccaagt
      301 catagtcgcc gatcttggga attaactcct ggattgcgtg cgcacacttg tccgattcat
      361 tgagcagggc ttcgagttgc cgggccaccg agtcatcttt catggcagcc cgcttacgcc
      421 ctttccccga ggcactgttc cgacagccgc ttggctaccg caaaggtatg ccggatgtca
      481 gcaagatcgg cttcaatctt ctccaaacgc ctttggaggt cgtagtcttt caccctgtgc
      541 attactctgt gaaggccgtg ggcgcgctta tccgtttccc tgagcatttc agccaacaac
      601 gcgccgagtt gctggccctt gatttcttcc atgatccacc tcactccact gctacattat
      661 aatacgcagt ccgcgcgccc cgcgcaaggt tatgtggttt caacgaagcg aaaagcattc
      721 tatttttctt ctcgacacac tttgggaaca acttcagatt ctgtacagtt ctcgtccgca
      781 atccgtgctg acttctggtc ttcgacgcct gttgcggcca agagactgta aaaaaggtca
      841 gaaaactaaa ggaggttgcc ggagcgcagg agacaaaatt agtatgtgaa aaaagtctca
      901 aacgagactt gacaggataa atcgagtctt ttattattcc agtattgaga tagggtctca
      961 aacactatgg caccgaaagt gaaactcttc gctgattacc tggcatccag gggtctgaag
     1021 ctgaccgggg agaggcagaa gatcgttatt gaaatcttcc gaacgccgcg gcattttgag
     1081 ccggaagagc ttttgattga tttgcgcggt cgccacctca aggcctcccg ggcaaccatc
     1141 taccgcacgc ttgagctcct gatggaagcc ggcctgatcc gcaaggtctg cctcggttcc
     1201 gggcgctttt cgtacgagcc ggtgtccgaa gacagggcgc acgaccacct catctgcgtc
     1261 cgctgcggca aggtggtctg tttcatcgat accgaggccg aggcggcaaa aaaacggatc
     1321 agcgccgaga agggatttga actgaccggc cagtgtttcc agttgttcgg gtactgcccg
     1381 gcctgccgga gctgaccttt ggccttttat ttatgaacct attgcaaatg aatctcaata
     1441 tcaaaaagaa cggagcaagg ttgtaatttt tttcacaaga aagatgactg ataagtcagg
     1501 aatcaaaacg ggaagccgaa ggaaaagaga ttcagtggtg cagcgtggga ttttgatggc
     1561 ctgcctttgc gtgtcctccg gagggctggc tgcggctcaa gaaacttcac cctaccactt
     1621 tcacggatac ggcgaaatgc atttcaaccg acccaagatc gggacaatgt cccagggagc
     1681 cggagatgaa attgacttcc accggatggt cttcgggttc ggttacgatt tttcagagaa
     1741 aattcgcctc gacttcgaac tcgattttga gcatgctttt accgaaccgg aattagagta
     1801 cgcgcaggtt gattttgacc tcttgcctgg gttgaccctc cgcggcggca atctcctgat
     1861 tccggtgggt cctttgaacg aattccacga gccgccgctt ttctacagcg tcgagcgacc
     1921 ctacgtccag cgcagcatca tccccaccac ctggcaggaa aacggtgcgg gtgtggtggg
     1981 gcgggtactg gataaccggc tggcctatcg ggcctacgcc gtctcggggc tggacggagc
     2041 caaaatcacc tctctggacg ggctgcacga cgtcagcagc aaaggggcgg aggccaaagc
     2101 caatgatttc gctggaaccg ggcgggtaga atttacgccc attcgtgagc tgaccgttgc
     2161 cgcctcgggc tactttggcg gagccgatca ggatgagccg ggtctgggca aagtggaggt
     2221 cgacatcgcc gagggcgact ttcactttca aattcaagac atcgacctta ccggagtttt
     2281 tgtacgtacg aaaatcaccg gagcacggca actcagcttg gcggcaggcg aaacgatcgg
     2341 caaggccatg cagggctggt acggcgaagc ggccgtccac actttgaagc gtttgctgcc
     2401 ggagacctcg cacgatttgg tgctcttcgg ccggtttgaa aagtttgaca ccaaccacga
     2461 agtcccggcc ggatttgtcc aaaaccttaa agccgaccgg gagatcttca ccttcggcct
     2521 ggcgtactat cccatcgaca gggtcacggt gaaaagtgac ttggaactct ggaaagacgg
     2581 caccgacgac acggtgagcc ggttcaatct cggcctggcc ttcatgtact gagggaaagg
     2641 agcagcgttc ttgaaaacgt ctcaaacatt cgcaagtttc ctggggttga gcctgtttct
     2701 gtccagtctc gcttgggccg gcgaaaagaa ggatatagct cagccctttg cccggctgac
     2761 cgtgaagttc aacggctgcg cctcctgcgc cggctgccgg agcgccatgc gccaggtggc
     2821 gcagaccaaa tcagcgcgcc atgcgcgtat ccagatcggg gacgatcgga gcctctcggc
     2881 cacttacccc aaggcgacga acatccccat gcgggagtta ccggcagcgc tgggaaaaag
     2941 cggcctgcat cagtttgaag tggccgaggt gaccctggag accaaaggca ccgtgaccga
     3001 aaaagacggg cagtttttcc tcattctgcc cgaaacgggt cagtgcctcc tcctttccgg
     3061 cctcgcaaat gcagaggaag tcaccggcca ggagctggcc ttgcggggga aagttcgcaa
     3121 ctggcagagc ccggaggaac aattagtcct ggacgtgcag tcggcccaga gcatagcgtc
     3181 ggccgcttcg gagagaaagt aggggttcag tgcccagcgc aaccggggaa gcaaaaattc
     3241 ccgcgccggt aaggaaaaat ctggcagagt ttactcccgg acaggtggga gtcgtttctg
     3301 aaattcacgg cgaggtggag ttgaccctgc ggatgatgga aatcggcctg gtgccgggag
     3361 tgcgcgtgcg ctacctgcgc gccgcgcctt tgggcgatcc gattcaggtc gaagtggagg
     3421 gttttttgct ctccctgcgg aaaagcgaag ccgaagcggt catcctgcat tccagcgagc
     3481 tgccatgaca gcgcaattga ccgaaaagaa cgttttcacg gttgccctgg tcggcaatcc
     3541 gaactgcggg aagaccaccc tctttaacgc cctgaccggc ctgcggcaga aggtggcgaa
     3601 ctaccccggc gtgacggtgg agaagaagac cggtctctgg gaaattgagg gaaccactgt
     3661 cgaagctctt gatcttcccg gcagttattc actggttccg cgctcccccg atgaaaaaat
     3721 ggttgcggac gtattgtcgg gaaggcgtcc ggatctgccc gcgccggatg ccgtcatctg
     3781 cgtcgtcgat gtaaccaatc tctcgcgtca cctttacctg gtcgggcagg tgattgaatt
     3841 aggtcgcccg acggtagtgg ccctgacgat ggtggatgag gcggagcgga taggccttac
     3901 cgtggacacc gccaaacttt ctgcccgttt gggtgttccg gtcgttgcag tgatcgccgc
     3961 gcgccgggtt gggctcgacc aactggctct ggcggtcaaa aaaggagtct tcagtcaatg
     4021 gcgatcaccg gagcggttct ggcggctgct cccggccttt gagaaccgcg taaaagcgta
     4081 tgcgccggaa gccgcagcga cggaattgga aacggtggcc tgcgtcgccg gactccacac
     4141 ccccgcctgc accgaaaagt tggaaaaagt cgcgaatcaa attcgttccg aattaggggt
     4201 gaacgaagag accgtccgcc gctacgtggt ggaagcccgc taccgctggc agcgggaggt
     4261 gacccgagaa gtcgtcgaga aaacggggga ccctcgggtc gattggacca aaagacttga
     4321 taagattttt ctgcactcca tccttggccc gctcgttttg ctggttaggt taacgc
//
LOCUS       DBNGZA010000452         1830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2210194, whole genome shotgun sequence.
ACCESSION   DBNGZA010000452 DBNGZA010000000
VERSION     DBNGZA010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2210194"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="AC16FU_09700"
     CDS             complement(<1..1126)
                     /locus_tag="AC16FU_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR2994682.1"
                     /translation="MDSFVEFKKKAAQGNVVPLVAEMPADLMTPVGAFLRVARRARYS
                     FLLESVEGGEKLGRYSFIGLDPYRLFACTQQGIEIREQRKRLALSTDPFAYLRESFNG
                     CQPAFDPRLPRFSGGAVGYFGYDAVRLFERVPDKNPDPLKLPEIFFGFYDLVLAFDHL
                     RQKVFVICNLFTDRKGEPLPRQYREGVRRVEAVLSLLSGPLPARRSATRPPRRAQVTS
                     NLSQEQFEAKAQAAKDLIAAGDIFQVVLSQRFQRGCAALPFAVYRALRMVNPSPYMFF
                     LKMDDVTLVGSSPEMLVRVDDGSVETRPIAGTRPRGQSEADDRRLAAELLVDPKERAE
                     HVMLVDLGRNDIGRVSQPGKVRVEDLMHIERYSHVMHIVTS"
     gene            complement(1129..>1830)
                     /gene="dapF"
                     /locus_tag="AC16FU_09705"
     CDS             complement(1129..>1830)
                     /gene="dapF"
                     /locus_tag="AC16FU_09705"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR2994683.1"
                     /translation="VLLRRGRRYPFRMEIYNADGSQAEMCGNGIRCLARLIYEAGHST
                     SRTQAIETRGGIIRTEITSLSNGQRPFTVRVDMGRPELAVKRIPVRSEQKHFVNQSVR
                     IGLQKINLTCLSIGNPHAVTFVRALPENWALLAQEIAALRLFPKGVNVSFASVQGRRK
                     MTLRVWERGAGATQACGTGACAAAVAAVLNGLAERTVKVHLPGGSLKVEWDQKSDHVF
                     LTGPAVRVFSGRFEF"
BASE COUNT          383 a          588 c          513 g          346 t
ORIGIN      
        1 ccgaagtgac aatgtgcatg acgtgggagt aacgctcgat gtgcattaaa tcttcgactc
       61 tgaccttccc gggctggctg acccttccta tatcgttgcg gcccaaatcg actaacatca
      121 cgtgctcagc ccgctccttg gggtcaacca aaagttccgc cgccaggcgg cggtcatcgg
      181 cttcgctctg gccgcgggga cgcgtgccag caatcggccg ggtctccacc gagccgtcat
      241 caacgcgcac cagcatctcc ggcgaggagc cgaccaaagt cacgtcatcc atcttcagga
      301 aaaacatgta cggcgacgga ttgaccatcc gcagcgcccg gtagaccgca aacgggaggg
      361 ccgcgcaccc ccgctggaag cgctgcgaca gcaccacctg gaaaatgtcg ccggcggcga
      421 taaggtcttt ggccgcttgc gctttcgctt caaattgttc ctgagaaaga ttggaggtga
      481 cctgtgctcg gcggggaggc cgtgtcgcgg accgccgcgc gggcaagggc ccggagagca
      541 gggagagaac cgcctccacc cgccgcaccc cctcccggta ctgccgcggg agcggttctc
      601 ccttccggtc ggtgaagaga ttgcagatca caaaaacttt ctgccgcagg tggtcgaagg
      661 ccaggaccaa gtcgtagaat ccaaagaaaa tctccggcag tttcagtgga tcgggatttt
      721 tatccggtac ccgttcgaac agccggactg catcgtaccc gaagtacccc accgcaccgc
      781 ccgaaaagcg cggcaggcgc gggtcaaacg ccggctgaca accgttgaag gattcccgca
      841 agtatgcgaa cggatcggtg gacaaggcga gtctttttct ctgctcgcgg atttcaatcc
      901 cctgctgcgt gcaggcaaac agccggtacg gatcgaggcc gatgaaggag taacgcccca
      961 acttctcccc gccttccaca ctctctaaga gaaacgagta acgcgcccgg cgggctacgc
     1021 gcaggaaggc gcccaccggc gtcatgagat cagccggcat ttccgccacc agcggaacca
     1081 cgtttccttg cgccgctttc tttttgaatt caacgaacga atccatacct aaaactcgaa
     1141 cctgccggaa aagacccgca ccgccggccc ggtcaaaaag acgtgatcgc ttttctggtc
     1201 ccactccact ttcagactgc cgccgggcag gtggactttg accgtgcgtt ccgcaaggcc
     1261 gttgagcacc gccgccacgg cggcggcgca ggccccggtg ccgcaggcct gcgtggctcc
     1321 ggctccccgc tcccagacgc gcaaggtcat ttttctccga ccttgcacgc tggcgaagct
     1381 gacgttgacg cctttcggga aaagccgcag cgcggcaatt tcttgagcca gaagagccca
     1441 attttccggg agtgcccgga cgaaagtaac cgcgtgtgga ttgccgatag ataaacaggt
     1501 caaattgatc ttctgcaaac caatgcggac cgattggtta acaaagtgtt tctgctcaga
     1561 gcgtaccgga attctcttta cggccagttc cggcctcccc atatccaccc gcaccgtaaa
     1621 aggtctttgc ccgttgctta gggaagtaat ttccgttcgg atgattccgc cgcgcgtctc
     1681 aatcgcctgc gtacgacttg ttgagtgtcc ggcttcgtaa atgagccgcg ccagacagcg
     1741 aatgccgttc ccgcacattt ccgcctgcga gccatcggca ttgtaaatct ccatgcggaa
     1801 gggatacctt ctcccccgcc gcaagaggac
//
LOCUS       DBNGZA010000453         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1151578, whole genome shotgun sequence.
ACCESSION   DBNGZA010000453 DBNGZA010000000
VERSION     DBNGZA010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1151578"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..893
                     /gene="ftsY"
                     /locus_tag="AC16FU_09710"
     CDS             3..893
                     /gene="ftsY"
                     /locus_tag="AC16FU_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR2994684.1"
                     /translation="MKSSLAKTKDNFLSKIAAVIGLHPKLDASLLQELEDTLIRSDVG
                     VAVTLRLVEQLKKRAQQNDLTGADAVISLLKDEMRHCLVNGSGAATTKEKPHVILVVG
                     VNGTGKTTTIGKLAYHFAGQGQKVLLAAADTFRAGAIDQLAIWAGRAGALLVKSTPGA
                     DPAAVAFDAVSAGLARGLDLVIIDTAGRLHTKHNLMEELKKIKRAITKVLPNAPHEVL
                     LVLDATTGQNGLAQAKLFTQAVGVTGLVLTKLDSTAKGGIIFAIASDLALPVKYIGIG
                     EQTDDFGLFEPDQFVEALFS"
     gene            890..1285
                     /locus_tag="AC16FU_09715"
     CDS             890..1285
                     /locus_tag="AC16FU_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR2994685.1"
                     /translation="MITRLQLRSTKREEALDITSKVELVVKQAGMAEGLCRLYVPHTT
                     ATIFINENEDPAVPKDIVRYLSELVPRGGDYQHREGNADAHIKAALIGATLEIPIQNG
                     KLLLGTWQGIFFCELDGPRQREVIIQLSH"
     gene            1450..2370
                     /locus_tag="AC16FU_09720"
     CDS             1450..2370
                     /locus_tag="AC16FU_09720"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR2994686.1"
                     /translation="MDPREIITKKRDGERLTEAEIDFLVSCYSRNIIPDYQMAAFLMA
                     VVLRGMDERETIALTKSMRDSGEVLDLSDIPGPNVDKHSTGGVGDKVSLVLAPLVAAC
                     GIVVPMVSGRGLGHTGGTLDKLEAIPGFRTNLSTAEFKRQLADIGVAMIGQTQSIAPA
                     DKKIYALRDVTGTVESIPLICASILSKKLASGAEGIVFDIKVGSGAFMKTRGEATRLA
                     KGLRSLSRAFGKKTAILFTGMDEPLGEAVGNAVEVIEAIETLKGGSTLLTTLSLSKVG
                     GPADLLTVTFALASRMLLMGKLAQTEHQAL"
     gene            2404..>2730
                     /gene="deoA"
                     /locus_tag="AC16FU_09725"
     CDS             2404..>2730
                     /gene="deoA"
                     /locus_tag="AC16FU_09725"
                     /inference="COORDINATES: protein motif:HMM:NF019448.7"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HIR2994687.1"
                     /translation="MAKFREMVKRQGGDERIVDDYSRLPQARYRISVPSAKSGWVKAI
                     DTYRVGMLAVELGAGRKQITDPIDHAVGFLIHKKVGQKVAAGEALATVLANDEEQGRE
                     VAQQLQQ"
BASE COUNT          623 a          762 c          798 g          547 t
ORIGIN      
        1 gattgaaatc gagtctcgcc aagacaaaag ataacttcct aagcaagatc gccgcagtta
       61 taggactcca tccgaagctt gatgccagtt tgctccagga gttggaagac actctcattc
      121 gctccgacgt cggcgtggcg gtcaccttgc gtttggtcga gcagttgaaa aagcgtgccc
      181 aacagaatga cctgaccggt gccgacgccg tgatcagtct tttgaaagac gaaatgcggc
      241 actgcctggt gaatggttca ggcgctgcaa ccacaaaaga gaaaccgcac gtcatcttgg
      301 tagtgggggt gaacggaacc ggcaagacta ccaccatcgg gaaactcgct taccactttg
      361 ccgggcaagg gcagaaggtg cttttggccg ccgccgacac cttccgggcc ggcgccatcg
      421 accagctggc catctgggcc gggcgggccg gggctcttct ggtgaaaagt acgccgggag
      481 ccgatccggc cgccgtggcc ttcgatgccg tctccgccgg cttggcccgg gggttggatc
      541 tggtcataat cgacacggcc ggccgtttac ataccaaaca taaccttatg gaggaattga
      601 aaaaaattaa gcgggccata acaaaagtct tgccaaacgc cccccatgaa gttttattgg
      661 tgctggatgc cacgacgggt cagaacggac tcgcccaggc aaaactcttt acccaggcag
      721 ttggcgtcac cggtttggtc ctgaccaaac tcgattcgac ggccaaagga gggattattt
      781 tcgccattgc ttccgatctt gccctgccgg tcaaatatat cggaattggc gaacagacag
      841 acgatttcgg cctgttcgag ccggatcagt ttgtggaggc gctcttttca tgattacccg
      901 gctgcaactg cgctccacca aaagggagga agccttagac atcacctcca aggtggagct
      961 ggtggtgaag caggccggga tggcggaggg gctctgccgg ctctacgtcc cgcacaccac
     1021 cgccacgatt ttcatcaacg aaaacgaaga cccggcggtg cccaaagaca tcgtgcgcta
     1081 tctctccgaa ctcgtgccca gaggcggcga ttatcagcac cgcgagggga atgccgacgc
     1141 gcacatcaaa gccgccttga tcggggctac gcttgaaata ccgattcaga acggcaagct
     1201 ccttctgggc acgtggcagg gaattttctt ttgcgaatta gacggcccgc gccagcgcga
     1261 ggtgatcatt caattgagcc actgagtttt agggcgtccg ggcagccgca gaccgcaact
     1321 caatcagatt attcaaatcg tggcttgcac ccccgcctga tttgagttcg ttcctgtggt
     1381 aaaaaacccc tcgttccccg taggcgcggc gcctcgccgc gcaaacttta gacctcccgg
     1441 taggtggtat tggatcccag agaaatcatt accaaaaagc gcgacggcga acggctgacc
     1501 gaagccgaga ttgactttct cgtctcctgc tactcgcgga acatcatccc cgactatcaa
     1561 atggccgctt ttttgatggc cgtcgtcctg cgggggatgg atgaacggga aaccattgcc
     1621 ctcaccaaaa gtatgcgcga ttccggggaa gtgctcgatt tgtccgacat ccccggcccg
     1681 aatgtggata aacattccac tggcggcgtg ggggacaaag tctctctcgt gctggcgcct
     1741 ttggtagcag catgcggaat cgtcgtgccg atggtttccg gccgcggtct ggggcatacg
     1801 ggcgggacat tggacaaact cgaagcaatt cccggcttcc gcaccaatct ctcgaccgct
     1861 gagttcaagc ggcagttggc cgacatcggc gtcgcaatga tcggccagac ccagtctatc
     1921 gctccggcgg acaagaaaat ctacgctctg cgggatgtta ccggcaccgt ggagtcaatt
     1981 cctttgattt gcgccagcat tctctccaaa aaacttgcct ccggagccga gggaattgtt
     2041 tttgacatca aagtcggcag cggcgctttt atgaaaaccc ggggcgaagc gacccgcctg
     2101 gcaaagggct tgcgttcgct ctcgcgcgcc ttcggaaaga aaacggccat cctgttcacg
     2161 gggatggatg agccgttagg ggaggcggtg ggcaatgcgg tggaggtcat cgaagccatc
     2221 gaaacgctga aaggtggttc tactttgctc accaccctga gcctgtcgaa ggttggcggc
     2281 ccggcggatt tgctcaccgt cacctttgcg ttggcgagtc ggatgctcct tatgggcaaa
     2341 ctggcacaaa cagaacatca agcactctag aagcttcgtc aggccataca gaccggagca
     2401 gctttggcca aatttcggga aatggtcaaa aggcagggag gagatgaacg gattgtggac
     2461 gactactcgc ggttgcctca ggccagatac cgcatatcag tcccgagtgc caaatccggc
     2521 tgggtcaaag ccatcgatac ctaccgggta ggtatgctgg ctgtggaatt aggagcgggg
     2581 cgaaaacaga ttaccgaccc gatcgatcac gccgtgggat tcctgattca caaaaaagtg
     2641 gggcaaaagg tcgcggcggg cgaagcgctg gccaccgtgc tcgcaaatga cgaagaacag
     2701 gggcgggagg tggcgcagca actgcaacaa
//
LOCUS       DBNGZA010000454         4546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_939268, whole genome shotgun sequence.
ACCESSION   DBNGZA010000454 DBNGZA010000000
VERSION     DBNGZA010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_939268"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="AC16FU_09730"
     CDS             complement(<1..423)
                     /locus_tag="AC16FU_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2994688.1"
                     /translation="MAGVDIGGGRPQRKQRLRRPKRRLGIRIDMTPMVDIAFLLLIFY
                     MVTTIFAQPQAMEINLPKDEKKDIEIAESNLLILRVAEDGKLYWNFGTKTPEELPFTE
                     LRKWLLDWNRAKPKLNTLIKIHKKAKYHNLVDIIDEIQV"
     gene            complement(500..982)
                     /locus_tag="AC16FU_09735"
     CDS             complement(500..982)
                     /locus_tag="AC16FU_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR2994689.1"
                     /translation="MLKRPKRRVAIRIDMTPMVDVAFLLLIFYMSTTQFKPPETKNVA
                     LPSSHQKAKLPDKDLVKVTVTKDDSIFVDYIEVREEKSADGSTFEVPTRVYEEATPAT
                     IASVINTIRLRNPRVFVVIRADKDTDYGTMEAMMKTMKENQLNRFQLLTEFEEEAEAS
                     "
     gene            complement(982..1710)
                     /locus_tag="AC16FU_09740"
     CDS             complement(982..1710)
                     /locus_tag="AC16FU_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR2994690.1"
                     /translation="MKQSIFITVLLIVALAVGYAVWEYLLPDYLKLGGPLVGLLIALS
                     IMLVAFIVERMLTLRKAKGHSAIPAYFKSVVEFLRKGDFEHALAACDKQRGSCANVLR
                     AGIDRYRQMAGNEPSAEKRLAETQRAIEEANALEVPLLERNLIAMSTIASIATMVGLL
                     GTTIGMIRAFRATGGHGGVIDAPQLAVGISEALVNTAGGLFNAIVGIVAYNFFVNKVD
                     SFNYTIDEASYEVLQLLKERETSR"
     gene            complement(1775..2491)
                     /locus_tag="AC16FU_09745"
     CDS             complement(1775..2491)
                     /locus_tag="AC16FU_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994691.1"
                     /translation="MSDPQKAVKEKRDTSSAPEAAKVLDPDLRFRYIGFEVHSPKIKN
                     FWKSAEEEKQYLEKIRREGSVWLKARSLVNVAIFSAADRALVTAISLVVLGSLFLPWA
                     SGAQRLWALPLFGELATHLSQAAGPVLLAAAVLGLALVATVPVVALANLVSVWRRGPT
                     EEKYAFRLRRVLLGNWLPIILWLLLLVGALVGAPLSAGAGAWVQAARFDFMAVMRTIG
                     LGLWVALGGHLVLAMKANEL"
     gene            complement(2488..4302)
                     /locus_tag="AC16FU_09750"
     CDS             complement(2488..4302)
                     /locus_tag="AC16FU_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994692.1"
                     /translation="MRRFSFDKNPGGQYIRPFMFVLRDPGKSLWSKRAIRSLLFGFFW
                     LGISVGGAVQAFGQNGSAAALAAYERKDYAAAVELFKQLAAKNPADPENYSYLGNCYY
                     HLGKFSDALAQYQAALSRKAKHWDALYGSGLTYLKLGQPGEAEQTFKKGTGDRAQKAR
                     FFNGLGFALQAQRKLDEADKAFRQAIVANPKVGEYHLNLAEVNFANGIYPIAVDEYRQ
                     ALVLDSTLSEARYRLAQALLKGRDFNGALEQLKEVLRADSTFAPAYFEMGDLYLKNTP
                     PSFKEAIWSLEHYIKLDTTNLSKAYLALAKAYYALRFYDSTMVKAKSVIARDPKNLEA
                     YFTLGQAASEGQKWSEAVEAYKRYQELLTEGKPDYPYSAKDAEFFQKRGEAYVGLKDS
                     TARPLAIADFEKALLLDSSRTQVFAPLGSAYIQSGDYERAIAWYQRALAADSTKFSTW
                     YNIGLAYQQRSRFAEAAEAFRHAVALKPDNVSARSRLAYILTVNLQNYGEGRQAFLEV
                     LKVDSLSADAHLYIGFAYLVEKKAADALPYLERSYHLRVNHQAKSPCDNTDNLLWLAQ
                     ASALSNRIDEAKKWAKKALECDSKNQPARELLKSLEPR"
     gene            complement(4295..>4546)
                     /locus_tag="AC16FU_09755"
     CDS             complement(4295..>4546)
                     /locus_tag="AC16FU_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994693.1"
                     /translation="SPPVAGREGSMGQVWVWTSYLILALIFGVGLFVLFSPGFARSFP
                     YQSGVRILVGLLLISYAGVRVWLTARRAAQSRAEGKWHA"
BASE COUNT          912 a         1380 c         1296 g          958 t
ORIGIN      
        1 cacctgaatt tcgtcgatga tgtccaccag gttatggtac ttggccttct tgtggatttt
       61 gatcaacgtg tttagcttcg gcttggcccg gttccagtcc agaagccact tgcgcagttc
      121 ggtgaacggc agttcctccg gcgttttcgt gccgaagttc cagtagagtt tcccgtcctc
      181 ggccactcgc aggatcagga ggttcgattc ggcgatttca atgtcttttt tctcatcctt
      241 gggcaggttg atttccatcg cctgcggctg ggcgaaaatg gtggtcacca tgtagaagat
      301 caaaagcaga aaggcaatat cgaccatcgg ggtcatgtcg atcctgatcc cgaggcgccg
      361 tttgggacgc cggagcctct gcttgcgctg gggtctcccg ccgcctatgt ctacaccggc
      421 catctgctac tcctcactgg taatacgcac caagtccacc tttattcccg gcccggctga
      481 ccagggtgcc cccacgccgc tacgaggcct cggcctcctc ctcaaattcg gtcaaaagct
      541 ggaagcggtt gagctgattc tccttcatcg ttttcatcat cgcctccatg gtgccgtagt
      601 ctgtgtcctt gtcggcccgg atcaccacga aaacccgggg gttgcgcagc cggatggtgt
      661 tgatgaccga ggcaatggtc gcgggcgtcg cctcttcgta gacccgcgtg ggcacttcaa
      721 aggtggatcc gtcggccgac ttctcttcgc gcacttcgat gtaatcgacg aaaatcgagt
      781 cgtccttggt cacggtcacc ttcaccagat ccttatccgg cagtttcgct ttctggtggg
      841 aggagggcag ggccacgttc ttggtctccg gcggcttgaa ctgggtggtg ctcatgtaga
      901 aaatgagcag aagaaacgcc acgtccacca tgggggtcat gtcgatccgg atcgccaccc
      961 ggcgtttggg ccgcttgagc atcagcggga ggtctcccgt tccttgagca gctgcagcac
     1021 ttcgtaggag gcctcgtcaa tggtgtagtt gaaactgtcg actttgttca cgaagaagtt
     1081 gtaggccacg atgcccacga tggcgttgaa caacccgccg gcggtgttca ccagggcctc
     1141 ggaaattccc acggccagct gcggcgcgtc gatcacgccg ccgtgcccgc cggtggctcg
     1201 gaaggcgcgg atcatcccga tggtggtacc cagaagcccc accatggtgg caatcgaggc
     1261 gatggtggac atggcgatca ggttccgctc caaaagcggc acttccagcg cattggcctc
     1321 ttcgatggcc cgctgcgttt cggctaaccg tttttcagcc gaaggttcgt tgccggccat
     1381 ctgccggtag cggtcgatgc cggcgcgcag gacgttggcg caggagccgc gctgcttgtc
     1441 gcaggccgcc agcgcgtgct caaagtcgcc tttccgcagg aattcaacga cgcttttgaa
     1501 atacgcggga atggcggagt ggcctttggc tttccgcagg gtgagcatcc gctccacgat
     1561 gaacgccacc agcatgatcg aaagcgcgat taaaagcccc accagcgggc cgcccagctt
     1621 gaggtagtcc gggagcaggt actcccacac ggcatacccc acggccagcg ccacgatcaa
     1681 caggaccgtg atgaagatcg attgtttcac tccttaccct cctactgtgg tttttctgct
     1741 ctagttcatt acgccagacg tttgttcgct cagatcacaa ctcattggcc ttcatggcca
     1801 gaaccaggtg cccgcccagg gccacccaga gcccgaggcc gatcgtccgc atcacggcca
     1861 taaaatcaaa acgcgccgcc tgcacccacg caccggcgcc ggcggacaag ggagcaccga
     1921 ccaaggcacc gaccaaaagc aacaaccata aaataatcgg cagccagttc ccgagcaaaa
     1981 ctctccgcaa ccggaacgcg tatttttctt cggtggggcc ccgccgccag acggagacca
     2041 gattcgccag ggccacaaca ggaactgttg caacgagcgc cagaccgaga accgcagccg
     2101 ccagaagcac cggtccggcc gcctgactca gatgggtagc cagttccccg aaaaggggca
     2161 gtgcccagag acgctgggca cccgaggccc acggcaggaa aagacttccc aaaaccacaa
     2221 gcgagatggc agtaaccagc gcccggtccg ccgcggaaaa tatggcgaca ttcaccaggc
     2281 tgcgcgcttt gagccagacg ctcccctcgc gccttatttt ttcgaggtac tgcttttcct
     2341 cttccgcgga cttccagaaa tttttgattt tcggcgaatg aacttcaaag ccgatgtacc
     2401 tgaaccggag gtccggatcg aggaccttgg ccgcctcagg cgcggaggac gtatcccttt
     2461 tttctttgac cgctttttgc ggatcgctca tcgcggttct aatgatttta aaagttcccg
     2521 cgccggctgg ttcttcgaat cacattccaa ggccttcttg gcccactttt tggcctcgtc
     2581 aatccgattt gacagggcgc tggcctgggc caaccagagg aggttgtcgg tgttgtcgca
     2641 ggggcttttg gcctggtggt tgacccgcag gtggtaggac ctttccaggt agggcaaggc
     2701 gtcagccgcc tttttctcaa ccagataggc aaacccgatg tagagatgcg catccgccga
     2761 aagcgaatca actttcagca cttccaaaaa cgcctgccga ccttcgccgt agttctggag
     2821 gttcacggta aggatgtagg ccagacggga acgcgcggac acgttgtcgg gcttcagcgc
     2881 cacggcgtgg cgaaatgcct cggccgcttc ggcaaagcgc gagcgctgct ggtaggccaa
     2941 gccgatgtta taccaggtgg agaatttggt tgagtccgcg gccaaggccc gctggtacca
     3001 ggcaatcgct ctctcataat ctccactctg gatgtatgcc gaccccagtg gagcaaagac
     3061 ctgtgtacgg gaggagtcga gcaaaagcgc cttctcaaaa tcggcgatgg ccaggggccg
     3121 ggcggtggaa tctttcaggc cgacgtaggc ctcgccgcgc ttctggaaaa actcggcatc
     3181 cttggcgctg tacgggtagt cgggctttcc ctcagtgagc agctcttgat accgcttgta
     3241 ggcctccacc gcctcgctcc atttttggcc ttccgaggcg gcctgcccca aggtgaagta
     3301 cgcctcgagg ttcttcgggt cgcgggcgat caccgactta gctttcacca tcgtggagtc
     3361 gtagaagcgc aaggcgtagt aagctttggc caaagccaga taggccttgc tgaggttcgt
     3421 ggtatcgagt ttgatgtagt gctccaggct ccagatcgcc tctttgaagc tgggcggcgt
     3481 gttcttcagg tacaggtcgc ccatttcaaa atacgcgggc gcgaaggtgg aatcggcccg
     3541 cagcacctct ttgagttgct ctaaggcacc gttgaaatca cgacccttca acagagcctg
     3601 ggccaaacgg tagcgtgcct ccgacagggt ggaatccaga accagcgctt gccggtactc
     3661 gtccacggca atgggataga tgccattggc aaagttgacc tcggctaagt tcaaatggta
     3721 ctcgcccact ttcggattgg cgacgattgc ctgccggaac gccttgtcgg cctcgtccaa
     3781 tttcctctgg gcttgcaaag cgaaacccag tccgttgaag aagcgggctt tctgggccct
     3841 gtcacccgtt cccttcttga acgtctgctc ggcttctccg ggctgaccca gcttcaggta
     3901 ggtcaaaccc gacccataga gcgcatccca gtgtttggcc ttccgcgaga gcgccgcctg
     3961 gtactgcgcc agggcgtcgg aaaatttccc caagtggtag tagcagttgc cgaggtagga
     4021 gtagttttcc gggtcggccg gattcttggc cgccagttgt ttgaataact cgacggctgc
     4081 agcgtagtct ttgcgctcat aagccgccaa ggccgcggcg gaaccgttct ggccaaaggc
     4141 ctgaactgcg ccgccaacgc tgatgcccag ccagaagaaa ccgaaaagca agcttcggat
     4201 tgcccgtttt gaccagagac tttttccggg gtctctcagc acgaacataa acggtcgtat
     4261 atactggccg ccgggatttt tgtcaaacga aaatctacgc atgccatttc ccttctgccc
     4321 ggctttgcgc cgcgcgccgc gcagtgagcc acacccgcac ccccgcgtag ctgatcaaaa
     4381 gcagcccgac cagaatgcgt acgcccgact ggtatgggaa agagcgagcg aaacccgggc
     4441 tgaaaaggac gaacagcccc accccgaaaa tcagggctaa aataagataa ctggtccaca
     4501 cccacacctg acccatactc ccttccctac ccgcgactgg tggcga
//
LOCUS       DBNGZA010000455         1739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_727064, whole genome shotgun sequence.
ACCESSION   DBNGZA010000455 DBNGZA010000000
VERSION     DBNGZA010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_727064"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="AC16FU_09760"
     CDS             <1..346
                     /locus_tag="AC16FU_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HIR2994694.1"
                     /translation="DRPDGYQPPGAIPQKHLIPLAEIIADAFGQGVDTKAVQEEYLRL
                     MRLLGSEFEILLDRTEEEIAKVAPPEVTEGIRRVREGKVSIVPGHDGVYGKISLFADG
                     EKIETPPQLSLL"
     gene            complement(362..1606)
                     /locus_tag="AC16FU_09765"
     CDS             complement(362..1606)
                     /locus_tag="AC16FU_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR2994695.1"
                     /translation="MQFALFFGILFFFAPSGRSAEPATLYVEARNDGRAGTGTEKNPY
                     RDLQTAIDRAKDGDQIMIRPGTYEAHSADYPESLCGNCQNHQTVVQATRGFLVQGKRV
                     HLVGSGPEKTTLVTKAGYGVLFDHSRGSVISGVRITGGVRDPDGNATDAGIVAKFSSI
                     TVAECEIGDNTNRIDTVVVGIGGVFGRENSELVIRDNLIKNNTWDGIALYRGATAFIT
                     DNVIDQGRGAGIGITWDASAIVLRNRISNYWKGIGTFGESRAVVRNNAVFDNLGWGIV
                     VTGSSFMEVTNNVIVRNGNCGLALWDSSASGKATNNVIVQNGWRKEWVCPCVGVVIWG
                     DSTKFPLSYNDVWNNVAGNWHEMPDQTGQAGNLSVDPLFLDKTDFHLQPASPLEDTGD
                     PYYTDPDGSRSDLGIYGGPGAR"
BASE COUNT          363 a          509 c          517 g          350 t
ORIGIN      
        1 cgaccgtccg gatgggtacc agcctcccgg ggccattcca caaaaacatt tgatcccatt
       61 ggccgaaatc attgccgatg ccttcggcca gggagtcgac accaaagcag tacaggagga
      121 gtacctgcgg ttaatgcgcc tgctggggag cgagtttgaa attctcctcg accgaacgga
      181 agaggaaatt gccaaagtcg ccccgccgga agtgaccgaa ggaatcagac gggtccgcga
      241 agggaaagtc tcaatagtgc cgggccacga cggcgtctac gggaaaattt ccctattcgc
      301 tgatggtgaa aaaatcgaaa ccccgccgca gttaagtctg ctgtaaaaag gtgaattcta
      361 cctaccgcgc tcccggcccc ccgtaaatcc ccagatcact tctcgaccca tccgggtcag
      421 tgtagtaggg atcgccagta tcttccaacg gcgaagcggg ctgcagatgg aaatcggttt
      481 tgtccagaaa gagcggatcg actgagaggt tacccgcctg cccggtctgg tcgggcattt
      541 cgtgccaatt cccggcgacg ttgttccaga catcgttgta cgagagaggg aatttagtcg
      601 agtcgcccca gatcaccacc cccacgcagg ggcagaccca ctccttgcgc cagccgttct
      661 gtacgatgac gttgttggtc gctttcccgg aagccgaaga atcccacagc gccaggccgc
      721 agttgccgtt gcgcacgatg acgttgttgg tcacttccat aaaggacgat ccggtgacca
      781 cgatgcccca gcccaggtta tcgaagaccg cattgttgcg caccacggcg cgcgactcac
      841 caaaggtgcc gatccctttc cagtagttgg aaatccggtt gcgcaggacg atggccgagg
      901 catcccaggt gatgccgatc ccggcgcccc gaccctggtc aatgacgttg tcggtgatga
      961 aggcggtcgc cccgcggtag agcgcaatgc cgtcccaggt gttgttcttg atgagattgt
     1021 cccggatcac cagttcggaa ttctcccggc caaagacgcc gccgatcccg acgacgaccg
     1081 tgtcgatgcg gttggtattg tcgccgattt cacactccgc cactgtgatg cttgaaaatt
     1141 tggcaacgat tccggcgtcg gtggcattgc cgtccggatc gcgcactccg cccgtgattc
     1201 gcacgccgga gatgaccgac ccgcggctat ggtcgaagag aacgccatat ccggctttgg
     1261 tcaccagggt cgttttttcc ggcccgctgc cgactaagtg cacccgcttc ccctgaacca
     1321 aaaatccccg ggtggcttga acgacggtct ggtgattctg gcagttaccg cagagacttt
     1381 ccgggtaatc agccgaatgc gcttcatacg tgccgggccg aatcataatc tggtcaccat
     1441 ccttggctcg gtcaatagcg gtctggagat cgcggtaggg atttttctca gtcccggtac
     1501 cggctctccc atcattgcgc gcctcgacgt agagtgtggc tggttcagca gaccgacccg
     1561 aaggagcaaa aaagaaaaga atcccaaaga agagcgcgaa ctgcactgca ccacgactga
     1621 ttgtctgaga ggcaatcatg aactctttct ttagtgcggc cgccggtgcg atggtcgcgg
     1681 gcgccggggc gcttgggtgt ggtgcggggg gtggggctgg gggtgctcgg gaggcaagg
//
LOCUS       DBNGZA010000456         4862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_303930, whole genome shotgun sequence.
ACCESSION   DBNGZA010000456 DBNGZA010000000
VERSION     DBNGZA010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_303930"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            397..1986
                     /locus_tag="AC16FU_09770"
     CDS             397..1986
                     /locus_tag="AC16FU_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR2994696.1"
                     /translation="MRRALRFLTATARKSRSRPYGPYGLRRIWTSLTTVLFVYASVGY
                     SQVPVLLYQKNGAPYDQLGWLVAGAGDVNGDGKADFIIGVPAATPGGLTNAGSAYVYS
                     GATGALLYQKNGAGANDRFGESVAGVGDVDGDGKVDFMIGAPHAYGASPNVGSAFVYS
                     GASGLLLYQKNGTGYNDQLGSHVVGAGDVNGDGRADFIVAARLADVGGFGDAGSVYVY
                     SGADGSLLYQKNGPSSQAWLGSSVASTGDVNEDGKPDFIIGAPGVSPGGLSFAGSADV
                     NSGADGALLFQIDGATAGSLLGLEVAGVGDVNDDGKADFGVGTFAPGFSDSGSVYVYS
                     GVDGSLLYQKKHPTLGNGYWGISVAGVGDVNDDRKADFIIGVAGSSSAFVYSGADGAL
                     LFQIDGAAPHDYFGYSVAGAGDVNGDGRADFIIGAPAPSFIYEPHIGSAYVYAFSCAP
                     RGDVAGAPITNCPDGTIDIVDIVAVINNVVFGTPFCGPEGAADVGGDPQGCPDTIVDI
                     VDIVSIINNVVFGTPFCQACL"
     gene            2460..>4862
                     /locus_tag="AC16FU_09775"
     CDS             2460..>4862
                     /locus_tag="AC16FU_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR2994697.1"
                     /translation="MKPRRLAALLIVFLLSAPGLSAYAPGRPISGQPGTATQRATVNF
                     ADIARREAAAPATTEELQDVPYPLPGPRNRQLPPGAVKGKDESSSLNRVNAPALTTVS
                     PPPAASFAALGDNNTRIPPDVHGAAGPSHLMTTLNSQVRIQNRTGGVISTVSLNSFWS
                     SLSSPSAFDPKILYDPYRDRWIFTSCSNSRSSLSSVLIGVTQTSDPTGTWNLYRIDAD
                     PANVAWADYPSFGFNNNWIVVQMNLFAVANDAFIRSNIYAFNKADLYAGGTGLFTLLV
                     DTVGGFSQAPAITYDTTLSTMYLVEDWFGNSGGNGMLRICTITGPVGSEVYTPDVSFP
                     TVADPWEDVGSTGFTDFAPQSGSVQKIMCNDARMQDCVYRNGFLWCTHTVFLPAGSGT
                     RSGVQWWKLSTAGSILQRGRIEDPSGAVFYAFPSLAVNKNDDVVIGHSRFSASQFASG
                     NYSFRAAGDPSNTFQTDTVLKVGEASYYKTFSGTRNRWGDYTGTVVDPVNDIDFWTIQ
                     EYAATPSGGFDRWGTWWGKVVPPAVPCAADTVRPNLTCPANIVKSNDIGQCNAVVTYT
                     ATAFDNCPGVTLACSPSSGSTFALGVTDVKCVATDAAGNKDSCVFTVTVNDTQKPTVA
                     CPANITLGNDPGQCGAAVTYSATAADNCAGVSLVCTPLTNSTFPIGATPVKCAATDAA
                     GNKDSCTFTVTVNDAEPPVVNCPADITVSNDPGQCGAVVNFTALFSDNCPGTTLSFTP
                     ASGSTLAVGTTLVTCVATDGGGNKDTCTFNVTVNDTERPVAQCRDTVVIADSGQQAQV
                     VNL"
BASE COUNT         1009 a         1368 c         1392 g         1093 t
ORIGIN      
        1 ccatggcaac cccaaattag cggtggaagg ggcacctgtg cgcccccatg acgaccccaa
       61 attagcgagg ctggaagcct cgcctatggg gagaggacat ccgttttacc cccgcgacaa
      121 ccccaaattt ctgagacatg ggggattagg gatcagtaaa gaaaagaact ggactcaagt
      181 cccgcctacg cggggagagg gaagaagcgg acgaatcccc ttaggcgaat tgaaccactc
      241 ctgtattgaa ctttccttga cagacaaaag aatccgaatt tattgattgt ggaataggtg
      301 gggatgcctc gaggaggaaa gaggcgtgat aagaaaacgg gggggcaaat tagtcttttg
      361 aaaccagcgg gtcgagatga cccgatggga gggtgtatgc gcagagcgct acgatttttg
      421 accgctaccg ctcgtaagag tcgaagccgc ccatacgggc cgtatggcct gaggcggatt
      481 tggacgagtc tgacgacagt tctgttcgtt tatgccagcg tgggctacag ccaggtgccg
      541 gtcctgcttt accagaaaaa cggcgccccc tatgaccagc tgggctggtt ggtagccgga
      601 gcgggagatg ttaacggaga tggcaaggct gatttcatca tcggtgttcc agctgccacc
      661 ccgggcgggc tcactaatgc tggttctgct tacgtctatt ccggtgccac tggtgccctg
      721 ctttaccaga aaaacggcgc cggcgccaat gataggttcg gcgagtctgt ggcgggagtg
      781 ggcgatgtgg acggagacgg gaaggttgat tttatgattg gggctcccca tgcttacggg
      841 gcctcaccta atgtcggctc cgcttttgtt tattcgggtg ccagcggcct cttgctttac
      901 caaaagaatg gcacgggata taatgaccaa ctgggcagtc acgtagtcgg agcgggggat
      961 gtcaacggcg acggtcgagc cgatttcatc gttgcggctc gtcttgcaga tgtaggtggc
     1021 tttggtgatg ccggttctgt ctatgtttac tccggagcgg acggctcgct gctttaccag
     1081 aaaaatggtc cgtcctcgca ggcctggctg ggcagttcag tagcctcaac gggagatgta
     1141 aacgaagatg ggaaacccga ttttatcatc ggggcgcccg gtgtctcacc gggcgggctt
     1201 agtttcgctg gctccgccga tgtcaattcg ggagcagacg gagctctcct tttccagata
     1261 gatggcgcca ccgctggtag tctgttgggt ctggaggtgg ccggggttgg agacgtcaac
     1321 gacgacggca aagcagattt cggcgtcgga actttcgctc cagggttttc agattcgggc
     1381 tctgtgtacg tgtactccgg agtggacggc tcgctgcttt atcagaaaaa gcacccaacc
     1441 cttggcaatg ggtactgggg gatctccgtc gccggtgtcg gggatgtcaa cgacgacaga
     1501 aaagctgact tcatcattgg ggttgcgggg tcttcctccg cttttgtcta ttccggcgca
     1561 gacggcgctc tccttttcca gatagatggt gctgcccctc atgactattt tggctattcg
     1621 gtagctggag cgggagatgt taacggagat ggcagagcgg atttcataat aggggctccc
     1681 gctcctagct ttatttacga accacatatc ggttcggctt atgtctatgc cttctcctgt
     1741 gcgccacgcg gggatgtagc gggggctcca atcacgaatt gcccggatgg gactattgat
     1801 attgtggata tcgtggcggt gattaacaat gttgtcttcg ggacgccctt ctgcgggccg
     1861 gagggtgcgg cggatgtggg gggcgatccg caggggtgtc cggacacgat cgtagacatc
     1921 gtggacatcg tctccatcat caacaacgtg gtgttcggga caccgttctg ccaggcgtgc
     1981 ctatgaaggg cagagattag agatcaggga tcaagaagat tcattcgacc tcaccctacc
     2041 ctctcctacg cggagaggga aaataaaaag gggcggggta aaccccgccc cggcacgaat
     2101 cttcgacccg ccgacgcggg ggaggggaat cgttttaacc ctgcgacctc aaacagtgcg
     2161 gttttcaggg atttggcagt atattgaagc ccagaaggaa ggtaattggc tgacagattt
     2221 agcattggaa tttttttgat tcaggacgaa aaaatccttg acaataatga atgatcggcc
     2281 acgttaggat tgtcggtgga actcaaaccg ccagcggaag tgcaaaacca gtttttttat
     2341 tcgccgccta cgactcgttc tcaaattgcg cgtgacgact gtggaggtcg ataattactc
     2401 tgtagggttc gataacggcc gattttccgt caaccgggaa tgtagaggag aatagttcga
     2461 tgaaaccacg ccgactggca gctttgctca tcgtattttt gctcagcgca cccgggcttt
     2521 ctgcttacgc tccgggacgc cccatctctg gccagccggg aacggccacc cagcgagcaa
     2581 cagtaaattt tgcggatatc gcccggcggg aagccgccgc tccggctacg accgaagaac
     2641 ttcaagacgt tccctatccg ctacccggcc cgcgcaatcg ccaactccct cccggtgccg
     2701 tcaaggggaa ggatgaatct tcttctctga atagggtaaa cgctccggca cttaccaccg
     2761 tttctccgcc tcccgccgcc agttttgccg ccctgggaga caacaacacc cggattcctc
     2821 ccgatgtcca tggggcggcc gggccgagcc atttgatgac gactttgaat tcccaggtgc
     2881 gcatccagaa ccggaccgga ggggtcatca gcaccgtttc gctgaacagt ttttggtctt
     2941 ctttaagcag ccccagcgcc tttgacccga aaattttgta cgacccgtat cgcgaccgct
     3001 ggatattcac ctcctgctcc aattcccgtt cttccttgtc gtccgtgctt atcggcgtga
     3061 cgcaaaccag cgacccgacc ggcacctgga atttataccg catcgacgcc gacccggcca
     3121 acgtcgcctg ggccgactac cccagcttcg gttttaacaa caactggatt gtggtgcaga
     3181 tgaacctgtt tgccgtggcc aacgacgctt tcatccgctc caatatctac gctttcaaca
     3241 aagccgacct ctacgccggg ggaaccggtc tcttcacgct tctggtcgat acggtgggcg
     3301 gattttcgca ggcgccggcc ataacctacg ataccaccct ttccaccatg tatctggtgg
     3361 aggattggtt cggcaactcg ggcggcaacg gcatgttgcg catctgcacc atcaccggcc
     3421 cggtagggtc ggaagtttac acgccggacg tttctttccc cacagtggcc gacccgtggg
     3481 aggatgtcgg ctccacgggc tttaccgatt ttgctcccca gtccggctcg gtgcaaaaaa
     3541 taatgtgtaa cgacgcgcgc atgcaggact gcgtctatcg caacggtttc ctctggtgca
     3601 cgcacacggt ctttttgccg gccggctccg gcacccgctc cggcgtgcag tggtggaaac
     3661 tgtccacggc cggctctatt ttacagcgag gcagaatcga agacccgagc ggggctgttt
     3721 tttacgcttt tcccagctta gccgtcaaca aaaacgatga cgtggtcatc ggccattccc
     3781 gcttttcggc ctcgcaattt gccagcggca actactcttt tcgcgcggcg ggcgacccgt
     3841 ccaacacctt tcaaaccgac accgtgctga aagtgggcga ggcctcttat tacaaaacct
     3901 tttccggcac ccgcaaccgc tggggagatt acaccggcac ggttgtcgac ccggtaaacg
     3961 acatcgattt ctggaccatt caggagtatg ccgccactcc ctcgggcgga tttgaccgct
     4021 ggggcacctg gtggggaaag gttgtcccgc cggcagttcc ctgtgccgcc gatacggtaa
     4081 gaccgaacct gacctgtccg gcaaacatcg tcaaaagcaa cgacatcggt cagtgtaatg
     4141 ccgtggtaac ttacaccgca accgctttcg ataattgccc cggagttacc ttagcgtgca
     4201 gcccctcttc cggctccact tttgcgctgg gagtaaccga tgttaaatgc gtggccaccg
     4261 acgctgccgg aaataaggac agttgcgtct ttacggtgac cgtcaacgat acccagaaac
     4321 cgactgtcgc ctgcccggcc aacatcacac ttggtaacga ccccggccaa tgcggcgctg
     4381 cggtaaccta ctctgctacc gctgcggaca actgcgcggg tgtttctctg gtttgcactc
     4441 cgttaaccaa ctcgactttc cccattgggg caacccccgt caaatgcgca gctaccgatg
     4501 ccgccggaaa taaggacagt tgcaccttta ccgtaaccgt caatgatgct gagccgccgg
     4561 tagtcaactg cccggccgat attaccgtca gtaacgatcc tggccagtgc ggtgctgtag
     4621 tgaacttcac cgctctattt tccgacaact gcccgggcac cacgctctct tttacgcccg
     4681 cttccggttc gaccttggca gtggggacga cactggttac ctgcgtcgcc actgatggag
     4741 gagggaataa agacacttgc accttcaacg ttactgtgaa cgataccgag cgtccggtgg
     4801 cgcagtgccg cgatacggta gttatcgccg attccggtca gcaggcgcag gtggtaaact
     4861 tg
//
LOCUS       DBNGZA010000457         2032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1152471, whole genome shotgun sequence.
ACCESSION   DBNGZA010000457 DBNGZA010000000
VERSION     DBNGZA010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1152471"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..>2032)
                     /locus_tag="AC16FU_09780"
     CDS             complement(230..>2032)
                     /locus_tag="AC16FU_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2994698.1"
                     /translation="LIVQLAHAGKYSPALLKQIADQQGVERIDLFGAKKNLLTSTTSD
                     EPFYLQQADVSALVDSVLWGYKKSIIVTLQPDPLHTEEHLALVAAAPGLAKAMVIVTS
                     LEFLGDFRREIGIGYLIQKIGQESGIEYVVLQNPEGIVLASRKVARMPKIESDLFLTE
                     ALGGDSVRTRLTDFDGHEVLEVVRPFRSQEIPGGLFRIGLSLEGLSQTAANFRRQVLL
                     ITLVIFLIGLLVVGLGVVNRNYAALERSFLDTKGLTQTVLEAMEGAVVAIDAKGLISV
                     FNPKAEKLFDRRAGEVTGKKYRELFPEDQFLLHQTVMTGKSTIGTERDFALSDGKRRM
                     LVSTARLADDTGTITGAVAVLSDVTQLRKLEEEAHRAERLSALGTMAAGVAHEIRNPL
                     NAISIAAQRLKTEFMPKEENDQYQKLTTTITTEVQRLNQTVNQFLSLARAQAVNKQKT
                     NLAELLNEVVAVAKMEAEPKKIGIQTEWAKNLPVALDKNEMKQALLNICLNGIQAMDT
                     GGTLAVKAGVDSSIDRQMKIEITDSGPGIAPESLSRLFQPYFTTKPGGTGLGLAIVQR
                     IVQDHQGKIEVESQVGKGTRFVVILPVEERERAF"
BASE COUNT          393 a          583 c          584 g          472 t
ORIGIN      
        1 atgccgtgag gcctgctcca gggcctgttc gcctgaactg caggcggtcg cgctgtagcc
       61 ctgcttggcc agatagccgg ccaaaagggt gcgctggggc tcttcgtcgt caacgactaa
      121 gatcgggtgt ttgcgcatgg ggttcaggaa ctcagccaat gacccctccc tgccctcccc
      181 tcaaagggga gggatgctga gagatcgatt ttgcccgttc acccggagtt cagaaggctc
      241 tttcgcgttc ctccacaggt agaataacca cgaaacgcgt acccttacca acttgacttt
      301 caacttcgat ttttccctgg tggtcttgaa cgattcgctg cacaatggcc agacccaggc
      361 cggtgccgcc gggtttggtg gtgaaatacg gctgaaacag acgactcaaa ctttccggcg
      421 caatgcccgg gccggaatcg gtgatttcga ttttcatctg acggtcgatg gaagaatcaa
      481 ccccggcctt gaccgcgagc gtgccgccgg tgtccatcgc ctgaatccca ttgagacaga
      541 tgttcaaaag cgcctgtttc atttcgtttt tgtccagtgc tacagggaga ttttttgccc
      601 actcagtctg tatgccgatc tttttgggct cggcttccat cttcgccaca gcgacaactt
      661 cgttcaacag ttccgccaag ttggttttct gcttgttgac cgcctgcgcg cgggccaggc
      721 tcaagaactg gttcacggtt tgattcaacc gctggacttc ggtcgtgatg gtggtggtca
      781 acttctggta ctggtcgttt tcctctttcg gcatgaactc ggttttcagg cgctgggccg
      841 cgatgctgat ggcgttcaga ggatttctga tttcgtgggc gacaccggcg gccattgtcc
      901 ccaaagcgga gagccgttca gcacgatgcg cttcttcctc taatttccgc aactgggtca
      961 catcggagag gaccgctacc gcgccggtaa tggtgccggt gtcgtcagcg agccgggcgg
     1021 tgctgaccag catgcggcgt ttgccgtcgc tcaaggcaaa atcgcgctcg gtgccgatgg
     1081 tactcttgcc agtcatcacc gtctggtgga gcaaaaactg gtcttccgga aaaagttctc
     1141 tatacttctt gccagtcacc tcacccgccc gccggtcaaa aagtttttcg gctttaggat
     1201 tgaagacgga gatcaaacct ttcgcgtcga tggccaccac cgcaccctcc attgcttcga
     1261 gcaccgtctg agtcagccct ttagtatcca ggaaagagcg ctccagcgcg gcgtagttgc
     1321 ggttcactac gcccaggccg acgactaaaa gtccgatcaa aaaaatcacc agcgtaatga
     1381 gcaacacctg acggcggaaa ttcgcggcgg tctgggagag tccttcgagc gaaagcccga
     1441 tacggaagag cccgccggga atctcctgtg agcgaaaggg ccgcaccact tccagcactt
     1501 cgtggccgtc aaaatcggtg aggcgtgtcc ggacgctgtc gccgcccagg gcctcggtca
     1561 aaaacagatc gctttcaatt ttgggcatcc gcgccacttt gcgcgaggcg agcacaatcc
     1621 cttccggatt ctgcaggacg acatattcaa tcccgctctc ctggccgatt ttctgaatta
     1681 agtagccgat gccgatttcg cggcggaagt cgcccaggaa ttcgagcgag gtgacaatca
     1741 ccatcgcttt ggccaagccc ggagcggcag ctaccagcgc caggtgctct tcggtgtgga
     1801 gcgggtcggg ttgcagggtc acgatgattg atttcttgta tccccagagg accgaatcga
     1861 ccagggcgct gacgtccgct tgctggagat agaacggttc gtctgatgtc gtgctggtga
     1921 ggagattttt cttggcgccg aagaggtcta tccgctccac gccctgttgg tcggcgattt
     1981 gtttgaggag cgcgggggag tacttgccgg cgtgcgccag ttgcacaatc ag
//
LOCUS       DBNGZA010000458         2631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2004430, whole genome shotgun sequence.
ACCESSION   DBNGZA010000458 DBNGZA010000000
VERSION     DBNGZA010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2004430"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16FU_09785"
     CDS             complement(<1..620)
                     /locus_tag="AC16FU_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR2994699.1"
                     /translation="MDAQFWRWKEYNWKAPDADWREAKSVTAGIDVGSVSSQAVILTD
                     GKIFAYSSQRTGSDSPQSAQKAMDWALDGTGLSLENIQFIVGTGYGRVNVPFAKKAIT
                     EIACHARGANFMYGSSVRTILDMGGQDCKAIRCDEKGKVTAFLMNDKCAAGTGRGMEV
                     FADLLSVPIEEIGDLSFTVDAEPPPVSCTCVIFAKSEALRLLRRGWA"
     gene            complement(629..1399)
                     /locus_tag="AC16FU_09790"
     CDS             complement(629..1399)
                     /locus_tag="AC16FU_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR2994700.1"
                     /translation="MTVAGIDIGAKTTKVLIAEDGRVLVQLLVLTGLETKQTVETAFE
                     QALQQASLSREKIARITVTGVGKQEFPLEHTEVSEVKADARGAVFLFPSARTVIDVGA
                     EEGRGIKCSQAGRVVDFTLNEKCAAGSGSFTEAMARALEVPLEELGELSRQSTQQIPM
                     NAQCAVFAESEVVSLLHAKTLKADIAKAVHDAIASRISSMARKIGIEQEVVLIGGVAK
                     NLGFIDSLKTDLSLHLLVPEEPEFVGALGAALIAQENG"
     gene            complement(1412..>2631)
                     /gene="bzdO"
                     /locus_tag="AC16FU_09795"
     CDS             complement(1412..>2631)
                     /gene="bzdO"
                     /locus_tag="AC16FU_09795"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA reductase, bzd-type, subunit O"
                     /protein_id="HIR2994701.1"
                     /translation="EKGGIRWCGGAWSFGAIPAGLGEDVFSLTGEPYAASIAWDKEFS
                     TQCLEAVEKKGFARDLCSYMRNYWGSILLKRYAFGGEFPKPDFVWQDHICCSHAKWYQ
                     VASELEGGIPMFCIDVACGPYTELNANKLDYVVGQMQDGIEWLEKITNRTFDDEKFIQ
                     AVYTECRSTALWAEICAFNKAVPAPLDEKSMFSLYVLGTLMKHSPIVADFYAELRDEV
                     ADRVKRGIAALAGERCRLMSDTQPPWGFLKIFRYLEEFGALSVGSLYSFGLIGMWEDQ
                     ADGSWGPRKTPQQKGIEIKDRETALRILAEWNLSKPEWQHFYSPRHKSQMMLRIAQEW
                     KVDGIMLHYNRGCEGLTLGIAENRLDLIKAGFPVMTFEGNMGDEREFDEQLVTSRIDA
                     FLENLNLKKLPHE"
BASE COUNT          554 a          804 c          676 g          597 t
ORIGIN      
        1 gcccagcccc gccggagcaa ccgcaaggcc tcggatttgg cgaaaatgac gcaggtgcag
       61 ctgaccgggg gaggttccgc gtcgaccgta aaggagagat ctccgatctc ctcaatggga
      121 acagacagta aatcggcaaa gacctccatc cctcggccgg tgcccgccgc gcatttgtcg
      181 ttcatcaaga aggcggtcac tttgcctttt tcatcacagc ggatggcttt gcagtcctgc
      241 ccgcccatgt ccagaatcgt gcgcaccgaa gagccgtaca tgaaattggc accccgcgcg
      301 tggcaggcaa tttcggttat ggcttttttc gcaaaaggaa cattgactct gccgtagccg
      361 gtgcccacga tgaactggat attttcgaga cttaacccgg ttccgtccag cgcccagtcc
      421 atagctttct gagcactctg cgggctgtcg gagccggttc tctggctgga gtacgcaaaa
      481 attttcccgt cggtcaaaat gaccgcctgt gaactgaccg agccgacatc gatgccggcg
      541 gtgacgctct tcgcctctcg ccagtcggcg tccggcgcct tccagttata ttctttccat
      601 ctccagaatt gtgcgtccat ctttccgcct acccgttctc ctgcgcaatc agagcggccc
      661 ctaaggcgcc gacaaactcc ggttcttccg ggacgagaag gtgcagactc agatcggttt
      721 tcagggaatc gatgaagccc aaattttttg ccacaccgcc gatcaagacc acctcctgct
      781 cgatccctat cttccgggcc atcgaggaaa tgcggctggc gatggcatcg tggaccgctt
      841 tggcgatgtc cgccttgagc gttttggcgt gcaaaagcga caccacttcc gattcggcaa
      901 aaaccgcgca ctgggcgttc atggggattt gctgggtgga ctggcgcgag agttcgccca
      961 gttcttccag cggcacctcc agcgcgcggg ccatggcctc ggtgaaagaa ccggatccag
     1021 ctgcgcattt ttcattgagt gtgaagtcaa ccactcttcc cgcttgactg cactttatcc
     1081 cccggccttc ttcggcaccc acgtcgataa cggtcctggc cgagggaaag aggaaaacgg
     1141 ctccccgggc atcggctttg acttcactta cttcagtgtg ttccagagga aactcctgct
     1201 tgccgacgcc ggtgacggtg atccgggcaa tcttctcccg ggagagcgaa gcttgctgga
     1261 gggcttgttc aaaggcggtc tcgaccgtct gcttcgtctc caagcccgtc aaaaccagaa
     1321 gctgaacgag aactcttccg tcctctgcaa tgagcacttt ggtggtcttg gcccctatgt
     1381 caattccggc tacggtcacc gccgcatcct ctcattcgtg tggcaatttc ttcaagttca
     1441 agttttccaa aaaggcatcg atccgggagg tgacgagctg ttcgtcgaat tctctctcat
     1501 cccccatgtt gccctcaaag gtcatcaccg gaaatccggc cttaatgaga tcgagccggt
     1561 tctccgcaat ccctaaggtc aacccttcgc agccccgatt gtaatgcagc atgatgccgt
     1621 ccaccttcca ttcctgcgcg atgcggagca tcatctggct cttgtggcgc ggggaataga
     1681 agtgctgcca ctccggttta gacaggttcc actcggctaa aattctgagt gcggtttcac
     1741 ggtcttttat ctcaatcccc ttctgctggg gggtcttcct tggaccccag ctgccatccg
     1801 cctggtcttc ccacatgcca atcaaaccaa aggaatagag cgagccgacg gagagcgccc
     1861 caaattcttc caagtacctg aaaattttca agaaacccca ggggggctgg gtgtcggaca
     1921 tgagccggca ccgctcaccg gccaaagcgg caatgcctct tttcacccgg tcggcgacct
     1981 catctcttag ttcggcatag aagtcggcca caataggact atgcttcatc aacgttccca
     2041 aaacgtaaag tgaaaacatg ctcttttcat ccaggggcgc cggcacggct ttattgaaag
     2101 cacaaatttc agcccagagc gcagtggagc ggcactcggt gtaaactgcc tggataaatt
     2161 tttcatcatc aaaggttcta ttggtgatct tctccagcca ttcaatgcca tcctgcatct
     2221 ggcccaccac gtaatccagc ttgtttgcgt tcagctcggt ataagggccg caggcgacgt
     2281 caatgcagaa catgggaatc ccgccttcca actcgctcgc cacctggtac catttggcgt
     2341 gactgcagca gatgtgatcc tgccagacga aatcgggttt gggaaattcg ccgccgaacg
     2401 catagcgctt taagaggatc gaaccccagt agttgcgcat atacgagcac aaatcccggg
     2461 caaacccctt tttttcaacg gcctccagac actgtgtaga aaactcctta tcccaggcta
     2521 tggaggcggc gtacggttct ccggtgaggg agaaaacatc ctcccccagg ccggccggaa
     2581 tggcaccgaa agaccaggct cccccgcacc agcggattcc ccctttttct t
//
LOCUS       DBNGZA010000459         1097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_516669, whole genome shotgun sequence.
ACCESSION   DBNGZA010000459 DBNGZA010000000
VERSION     DBNGZA010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_516669"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16FU_09800"
     CDS             complement(<1..592)
                     /locus_tag="AC16FU_09800"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR2994702.1"
                     /translation="MDYSLSELMIAAAAREIHDREIVFVGMRLPLLAFLVAKATHAPN
                     TIGLYENGIIRQKPAPELLYTMGDPPNVLGATFCGQMLEVMSFLQQGKIDVGFIGAGE
                     VDRFGNLNSSYVRDGNRLTRLPGSGGACDIASLAKRVVVILPHEKRRLKEQVDYVTSH
                     GYGTGNGWRKKVGLPRGGTSVVITTKGVLRFDEQSGE"
     gene            complement(669..>1097)
                     /locus_tag="AC16FU_09805"
     CDS             complement(669..>1097)
                     /locus_tag="AC16FU_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR2994703.1"
                     /translation="DAFNMPLETWPDLVLFSFLIDRVGVYQLREFVNSSYAPLARAVP
                     KMVEEKTIHMHYGQNLLTELCRTDDGKKQAPTALRKWYPRALDMFGSADSKRNLQYRQ
                     FGLKKKSNEEARQSYIADIGSILQQAGLGVPPPEFDRRIL"
BASE COUNT          225 a          311 c          310 g          251 t
ORIGIN      
        1 cctcgcccga ttgttcatcg aatcgtagga cgcccttcgt ggtgatgacg accgacgtgc
       61 cgccgcgggg caatccgact ttcttccgcc agccgttgcc cgtgccgtag ccgtgggagg
      121 tgacgtagtc aacctgctcc ttcaaccgcc tcttttcgtg cggcaggatg accaccaccc
      181 gcttggctaa agaagcgata tcacacgccc cgccgctgcc tggcaggcgg gtcaggcggt
      241 ttccatcccg cacataggat gaattaagat tgccgaagcg gtcgacttcc ccggcgccga
      301 tgaagccgac atcgattttc ccctgctgaa gaaaactcat cacctcaagc atctggccgc
      361 agaaggtggc gcctaatacg ttgggcgggt cgcccatggt gtagagcagt tccggcgcag
      421 gtttttggcg aataatgccg ttctcgtaga ggcctatggt atttggtgcg tgggtcgctt
      481 tggcgaccag aaatgccaga agcggcaggc gcattccgac gaagacgatc tcgcgatcgt
      541 ggatctcccg cgcggcggcg gcaatcatca gttcggataa ggagtaatcc atcgttcaag
      601 agggaatgac ttttttcttt atcgatttta agatttcaac ttcgatacgc cgacacttcg
      661 acactttttc agaggatgcg ccggtcaaac tccggcgggg gcacccccag cccagcctgt
      721 tgcaatatcg atccgatgtc ggcaatgtaa ctttgccggg cttcttcgtt actttttttc
      781 ttcagaccga actggcggta ctgcaggttg cgtttcgaat cggcggagcc aaacatatct
      841 aaagcccggg ggtaccactt tcggagggcg gtcggcgctt gctttttgcc gtcgtcagtc
      901 cgacagagtt cggtcaaaag attctggccg tagtgcatat ggatcgtttt ctcttccacc
      961 atcttgggca cggcgcgggc taaaggggca taactggagt tgacgaactc gcgcaactga
     1021 tacactccca cccggtcaat aaggaaagag aacagcacaa ggtccggcca ggtctccagc
     1081 ggcatattga aggcgtc
//
LOCUS       DBNGZA010000460         5572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2320220, whole genome shotgun sequence.
ACCESSION   DBNGZA010000460 DBNGZA010000000
VERSION     DBNGZA010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2320220"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="AC16FU_09810"
     CDS             <1..947
                     /locus_tag="AC16FU_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR2994704.1"
                     /translation="VGNSRPDPSLTLPSRGGKVITTSSREGKFRYRILSLPPILENIE
                     GRNMRSVLRILPLLIVAGFVCTCSKRTADTFARIQKTRILRVGNDPTYPPFESKDTHT
                     GKIVGFDIDLIDAICTKLDVRAEYVEAPFDGIIAGLNTDKYDCVISSMSIKPERAQVV
                     DFSLPYYQAGQAIAVRLKDEKIQGVEDLKGKRIGCQLGTTGETFARTQIPDGQVISFD
                     AISQAFLDLENGKLDAIINDRPTTERFIALKGTAKIVGPTLTSEDYGIAVRKGDARLL
                     TAINNALNSLRTAGKFDEIKAKWFGAPRAGQPAKTPAS"
     gene            1031..1107
                     /locus_tag="AC16FU_09815"
     tRNA            1031..1107
                     /locus_tag="AC16FU_09815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1065..1067,aa:Arg,seq:acg)
     gene            complement(1313..2170)
                     /locus_tag="AC16FU_09820"
     CDS             complement(1313..2170)
                     /locus_tag="AC16FU_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994705.1"
                     /translation="MKRWGVLAVGLLLLFLVGCFDLKEEVWINADGSAKIMQDIILPE
                     AMTSLGGEQANLAEQLNKQYGEKKSRLEADPRIKSVDTRHFTEGDKHHFVLEVEIKDV
                     TDLPEIWPLAFANKPDAAGKGSDSPLANQENRLSVSKTGDGNIHLTRVLKSAEKTGKK
                     DSTNQMGDAMGQAMAAQLFANNGITFTVHGSKIAQANGTILDDGQTAEWKIPLTEMMG
                     KNFSKEMSAEIGPGGAGLPTWIPIAAGGFLVLLVLLILITRNRKKKSSRFDNVPVIRP
                     STQKPVGVQ"
     gene            2431..2913
                     /gene="tadA"
                     /locus_tag="AC16FU_09825"
     CDS             2431..2913
                     /gene="tadA"
                     /locus_tag="AC16FU_09825"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR2994706.1"
                     /translation="MSHLLSGNNDETWMQAALKEAARAFDKGEVPVGAVAIYKNQIIG
                     RGHNQVETLKDATAHAEILALTAAMQYLDSWRLEEVTLYATLEPCCMCAGAVVLARVP
                     RLVFAAFDPKAGACGSLFNLVADPRLNHRVVVAHSVSAEESQALLQEFFRKLRVEEKS
                     "
     gene            2951..3424
                     /locus_tag="AC16FU_09830"
     CDS             2951..3424
                     /locus_tag="AC16FU_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994707.1"
                     /translation="MNSKPFDSDALHAKLAYLGLAIDVTLPIVLLAVGYAIRKDQPSA
                     LDYPTLRLLWVVLLAIAAGEFAVGLVLRKIWFAPQRFRQAVERAGGRADAVILRYAIA
                     LYAVSAAPAVYGFIYYLLGGTIEQFVGFLAITLLCYQILRPRPGLVDPLLKNSLS"
     gene            3585..3671
                     /locus_tag="AC16FU_09835"
     tRNA            3585..3671
                     /locus_tag="AC16FU_09835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3619..3621,aa:Ser,seq:cga)
     gene            3832..4449
                     /locus_tag="AC16FU_09840"
     CDS             3832..4449
                     /locus_tag="AC16FU_09840"
                     /inference="COORDINATES: protein motif:HMM:NF020496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HIR2994708.1"
                     /translation="MKLAKLRETKSTIRFSAKLFRPKATEKIGSWTLLTLPKNASAKL
                     PSRGMTMVEGTINGFPFRAALEPNGKGSHWLRVNKAMHDAAGADAGDTVTVEITRAGE
                     EPETRVPMDLRKALAVAPPAHASWTDITPMARRNWIFSISSAKQPETRRRRIEKACDM
                     LASGKRRLCCFPGIKWLMKKNAKSCGMWLPLPNSKNRFSPRLPKG"
     gene            4493..4921
                     /locus_tag="AC16FU_09845"
     CDS             4493..4921
                     /locus_tag="AC16FU_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR2994709.1"
                     /translation="MRFPIEIKAPREKVWNTLWEDKTFREWGNIIDEGQYMVGEMKEG
                     NEVQFISPVSGYGVTSLIEKLTPNEFVLFRQIMDTKESGTQEREKEWTGGAESYSLVE
                     KDGVTTLVVELDVPRGQEETFRVRFPKALERVKILAEKKD"
     gene            complement(5087..5338)
                     /locus_tag="AC16FU_09850"
     CDS             complement(5087..5338)
                     /locus_tag="AC16FU_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2994710.1"
                     /translation="MTALVSELTGARFAVCGKGIFKEEFVTCGGVNLKEVDFKTMQSR
                     RCPGLYLAGEILDIDGITGGFNFQSAWTTGWIAGANMAW"
BASE COUNT         1271 a         1528 c         1517 g         1256 t
ORIGIN      
        1 cagttgggaa ctcgcgtcct gacccctccc tcaccctccc ctcaagggga gggaaggtca
       61 ttaccacttc ctcccgagag ggaaagtttc gctaccgaat tctttcactg ccgcctatct
      121 tagagaatat cgaagggagg aacatgcgtt cggtcttgcg cattttgccg cttttgatcg
      181 tcgctggttt cgtctgcacg tgcagtaaga gaaccgccga cacttttgcc cgtattcaga
      241 agacgagaat cctgcgggtg gggaacgacc cgacctatcc gccgttcgaa tcgaaagaca
      301 cccacaccgg caaaatcgtg ggcttcgaca tcgatctgat agatgccatc tgcacgaaat
      361 tagacgtgcg ggcggagtac gttgaagcgc cctttgacgg cattatcgcc ggattgaaca
      421 ccgataaata cgactgcgtc atctcctcaa tgagcatcaa gcccgagcgg gcgcaggtgg
      481 tcgatttttc cctgccatac tatcaggccg ggcaggcaat agcggttcgg ctgaaggacg
      541 agaagattca gggggtagag gacctcaagg gcaaacggat cggctgccag ttgggcacca
      601 ccggtgagac atttgcccgc acccagattc ctgacggcca ggtcatctcc tttgacgcca
      661 tcagccaggc gttcctcgac cttgagaatg gaaaactcga cgccatcatc aacgaccggc
      721 ccacgacgga gcggttcatc gcgctcaaag gcacggccaa aatcgtgggc ccgactctga
      781 ccagcgaaga ttacggcatc gccgtccgca agggagatgc gcggcttttg acggccatca
      841 acaatgcttt gaactcgctc cgcactgcgg ggaaattcga cgagattaag gccaagtggt
      901 ttggtgcgcc aagggcgggg caaccggcaa aaacccccgc ctcgtaacct cgcggcggcg
      961 cagttcggtc gcttggccgg acagatttcg cttttgattc gtttggccgg gatttatatt
     1021 tgccttcgcc gtccgcgtag ctcaattgga tagagcatca gactacggat ctgagggttg
     1081 ggggttcgac tccctccgcg gacgccaatt ttatgagaag tatttcccac actcaggcct
     1141 tcgactccgc tcaggctgaa gagagcgttg ctaccgcctg gtttttgcgg gctaacttct
     1201 agtagcgacg gtctctgacc gtcggggtta catttttccg ggtcagtcag aggttagcgc
     1261 agttcggctg cgctcccaag cccacgtaac ttcacgggag agggaacggc acctactgta
     1321 cgccgacggg tttttgcgtc gaaggacgga tcaccgggac gttgtcaaaa cgggacgatt
     1381 tctttttccg gttccgggta ataagaatca aaaggaccaa aagaaccagg aagccgccgg
     1441 cagctatcgg tatccacgtc gggaggccgg cgccccccgg cccgatttcg gcgctcattt
     1501 cctttgaaaa gtttttgccc atcatttcag tcaaagggat tttccactcc gccgtttggc
     1561 cgtcatccaa aatcgttccg ttggcttggg caatcttcga cccgtgcacc gtgaaggtga
     1621 tcccgttatt ggcaaagagc tgtgccgcca tggcctgacc catggcgtcg cccatctggt
     1681 tcgtggaatc tttcttaccc gttttttcgg ctgacttcag tacccgcgtc aggtgaatgt
     1741 tgccgtcacc cgttttgctg accgaaagcc ggttctcttg attcgccaat ggcgaatccg
     1801 atccttttcc cgccgcatcg ggcttattgg caaacgccag cggccagatc tccgggaggt
     1861 cggtcacatc tttgatttcc acttcgagga cgaagtggtg tttgtcgccc tcggtgaaat
     1921 gccgggtgtc gacggactta atgcgcggat cggcttccag acggctcttc ttttccccgt
     1981 actgtttatt taattgctcc gcgaggttcg cctgctctcc ccccagagag gtcatcgcct
     2041 cgggcaggat gatgtcctgc atgatcttgg ccgagccgtc ggcgtttatc cagacttctt
     2101 ctttcaaatc gaagcagccg acgaggaaaa gcaagagaag accaaccgct aaaacacccc
     2161 accgtttcat aacgcctcct ttgtgttacc gatgtttttg gtatcggagt acttacacat
     2221 ttcttgagag tttacactgg cgttaaacta cctttgaaga cctcacccca ccctctccta
     2281 caggaaggag tataacttcc ctatgaatga aggggcaaaa ttccctcccc gttaggggaa
     2341 gggcgagaga gaggtcggct tccttcctgt accaccactc tgagccgccg atcttgcagc
     2401 ggccatttga agttgtatct tcacggcacg atgagccatc ttctctccgg caataacgac
     2461 gaaacctgga tgcaggcggc gctgaaagaa gccgcccgcg ccttcgataa gggagaggtg
     2521 ccggtaggcg cggtcgccat ctacaagaac cagattatcg gccgggggca taatcaagtc
     2581 gaaaccctca aggatgcgac ggcccacgcc gaaattctgg ctttgaccgc cgccatgcag
     2641 tatctcgatt cctggcggct cgaagaggtg acgctctacg ccacgctgga gccctgctgt
     2701 atgtgcgcgg gggcggttgt gctggcgcgg gtgccgcgtt tggtcttcgc cgcttttgac
     2761 ccaaaagccg gcgcctgcgg gtcgctcttt aatctcgtgg ccgacccgcg cctcaaccac
     2821 cgggtggtgg tggctcactc ggtatcagcc gaagagtcgc aagccctgtt gcaggagttt
     2881 ttccgcaaac tacgcgtgga agaaaagtct tgactttccc tgtgtgccgc gtgctgttta
     2941 ctgtgtactc atgaactcca aaccttttga ctccgacgcc ctccacgcca aactggccta
     3001 ccttggcctg gccatcgatg taactctccc gatagttctt ttagccgtcg ggtacgccat
     3061 ccgaaaagac cagccgtcgg ctttggacta cccgacactc cgacttttgt gggtcgttct
     3121 cttggcaata gctgcgggtg aattcgctgt cggcctggtg ctccgcaaga tctggtttgc
     3181 gccccagcgc ttccggcagg cggtggaacg ggccggcggc cgagccgatg ccgtgattct
     3241 tcggtacgcc atagcactct acgctgtttc tgccgccccg gcggtttacg gttttatcta
     3301 ctatctgctc ggcggcacca ttgagcaatt tgtcggcttt ctggcgatta ccctcctctg
     3361 ctaccaaatc ctgcgtccgc ggccgggact ggtcgatccg ctcttaaaaa attctctctc
     3421 gtagtggttc acattctttc cgaggcgcca gaggattaac ccctcccttg ccctcccccg
     3481 ccagaggcgg ggtcttcgac cctcgaaggg gagggaatca gaattggatg ggacagcaag
     3541 gcaacaacct tttccggctg tcttgggctt gctatactgt gggtggagag gtgcctgagc
     3601 ggccgaaagg ggcggtctcg aaaaccgttg tcccctcacg gggaccgtgg gttcgaatcc
     3661 caccctctcc gttgaaaacc ctgagcgaag gcgggaatcg gagcggaaag gcaaactgcc
     3721 aaagaacctg tcaaaaatgt cggctcaaac tttatgaagg ccagaagcgg gcttttcttg
     3781 aataaaacag aaaattagat atgatgatca tacagagtag tataacaata tatgaaactg
     3841 gctaagctgc gagaaaccaa gtcaacgatt cgcttcagtg ccaagttgtt tcggcctaag
     3901 gcaaccgaaa agattggatc gtggaccctt ctaactctgc ccaagaacgc gagtgcgaag
     3961 cttccatcac gaggtatgac aatggttgag ggaactatca atggcttccc ttttcgagcc
     4021 gctctcgaac caaacggcaa aggaagtcac tggctcaggg ttaacaaagc catgcacgat
     4081 gctgcaggcg cagacgctgg agacacggta acggtggaga ttacgcgggc cggagaagaa
     4141 ccggagacca gagtgccaat ggatctacgc aaagcccttg cagttgctcc accggcgcac
     4201 gcatcgtgga cggatattac accgatggca cgccggaact ggattttctc gataagctca
     4261 gccaagcaac cggaaacgcg caggcgccgg atcgagaaag cttgcgatat gcttgcatct
     4321 ggaaagcgac gattatgttg ttttcccggt ataaagtggc ttatgaaaaa aaacgcaaaa
     4381 tcatgcggaa tgtggcttcc attgccgaac tcaaaaaatc gtttttcgcc aagattgcca
     4441 aagggttagg cgtttcaatg aaagatttga taaaatgaac tttatgcaaa aaatgcgatt
     4501 tccaatcgaa ataaaagctc ccagagaaaa agtttggaac acactttggg aggacaaaac
     4561 atttcgtgaa tggggaaata ttattgacga gggacagtac atggtcggag aaatgaaaga
     4621 aggaaacgaa gtgcagttta tttcccccgt atccggttac ggcgtgacga gtttgatcga
     4681 gaagctcact cctaacgagt ttgtattgtt taggcagata atggatacaa aggagagtgg
     4741 aacgcaggaa cgggaaaagg aatggactgg cggagcggag agttattcac ttgtggagaa
     4801 agacggcgtt acaactttgg ttgttgaact cgacgttccg cgcgggcagg aagaaacatt
     4861 tagggtcaga tttcccaaag ctttggagcg ggtgaaaatt ttagcagaga aaaaggatta
     4921 aagaatttgc cgccaaagaa aaacttgaaa tcgtcctttc cgctccggtt caaggcgagg
     4981 cgggcggaag gggggtgtgg ggggaattcc gcccgccgag cccagccagt tcggttcgga
     5041 tattttcaaa cagacaccgc ttgagggcgt aaaacgcccg gtccacctac caggccatat
     5101 tcgctccggc tatccagccg gtggtccagg cgctctggaa attaaatccc cccgtaattc
     5161 cgtcaatatc cagaatctcc ccggctaaat acagcccggg acagcgccgg ctctgcatcg
     5221 ttttaaaatc cacttccttc agattaaccc cgccgcaggt cacaaattct tccttgaaaa
     5281 tcccttttcc gcaaacggca aagcgagctc cggtcagttc ggagacgagt gcagtcattg
     5341 cccgcttgga aaagttcgtc caggtggttt ccccggcaat tccggcaaat tgaacgaccc
     5401 ggctccagta tcgagccgga attgggaaaa cctattcgga ttgaacccgc ttccggccat
     5461 tttgtccctt gaaagcaacc agctcccggt acagctctag ggcgctgtaa tcgggcagca
     5521 aattgaccgt tagctccgcg caatagttgc agtgccggag catctgcgcc cc
//
LOCUS       DBNGZA010000461         3445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1366520, whole genome shotgun sequence.
ACCESSION   DBNGZA010000461 DBNGZA010000000
VERSION     DBNGZA010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1366520"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /gene="fusA"
                     /locus_tag="AC16FU_09855"
     CDS             complement(<1..2013)
                     /gene="fusA"
                     /locus_tag="AC16FU_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR2994711.1"
                     /translation="MREYTLEQIRNVAFVGHGGSGKTSLAEAALFAAKVTTRLGKVDD
                     GTTISDYSPDEITRKISISSTLLHFDWNGTKFNVVDLPGYTDFIGEVHGGLRVCETAV
                     VAVNAASGVEVGTEQVWQLGDRYGTSRVIFVNKIDKEHADFAKVLAVLQERFGHLVVP
                     VQLPLGSGPGFGGVIDLLQNKAFRFTGGTAKAEEIPAEFKNAAADARQKLLEAAAEAD
                     DSLLEQFFEAGELTDVQIQYGLKKGLAAKKIFPVLVGAATASAGTSLLVDFLANFAPS
                     PVDVPEVKGILSGTEQEVVRKTLPSEPVCALIFKTISEPHVGELSFFRVFSGHVKTGD
                     DIFNATRQHSERVGQIYAMNGKERKEVGTVSAGDIGAFVKLKNTHTGDTLCDRKAPIL
                     LPPVDFPKPVINMAIKPKAKTDDEKIASGLARLHEEDPTFSVEVNSDIKQTIVHGQGE
                     LHLEVIIDRLKRKYGVEVELEKPRIPYRETIKSKTEVQGKYKKQTGGHGQYGDCWLRI
                     EPLKRGTGFEFEDEIVGGAVPGKYIPSVEKGVVEAMQEGGLAGYRVVDVKVTIFDGSY
                     HTVDSSDIAFKVAGSMAFRSGFLQCKPVLLEPIYHVAVTVPDEYMGDVMGDLSARRGK
                     ILGMERNGASQTIKALVPLADLYKYSTALRSLTQGRGLHRREFSHYE"
     gene            2144..3088
                     /gene="argF"
                     /locus_tag="AC16FU_09860"
     CDS             2144..3088
                     /gene="argF"
                     /locus_tag="AC16FU_09860"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR2994712.1"
                     /translation="MPPASNTSRRGKRDFLTVTDFSAREISETLMLAQSLKKGKTRPQ
                     PLKGKSVACIFHKPSLRTRVSFETGIFQLGGHPIYITEKEIELGRRETIADAARVFSR
                     YVALIVIRTFAQKDVEELAKYATVPVINALTDLSHPCQIMGDLFTIWERRKNLKALSV
                     AYLGDGNNVANSWLELAARIPLDLRIGTSPQTMPDEETFRRAQETGISKIRITADPFS
                     AVQGVDVVYTDVWASMGQKHLAEEKAQFLSRFQVNSALLKHAKANALVMHCLPAKRGQ
                     EITDEVMDGPQSVVFDQAENRLHIQKAIMVKLLHGSKM"
     gene            3242..>3445
                     /locus_tag="AC16FU_09865"
     CDS             3242..>3445
                     /locus_tag="AC16FU_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994713.1"
                     /translation="MLPEIVKYSDCFVCGDKNPIGLKVKFFAKGDTAVAEYTVGDLYE
                     GYKGLFHGGLIAALLDEIMIKAVL"
BASE COUNT          671 a         1068 c          926 g          780 t
ORIGIN      
        1 ttcgtagtgc gaaaattccc gccggtgcag gccgcgcccc tgggtgagtg aacgcaaagc
       61 cgtcgagtac ttgtagagat ccgctaaggg caccagcgcc ttgatggtct gggaagctcc
      121 gttccgctcc atccccaaaa ttttcccccg ccgcgccgag agatcgccca tcacgtcgcc
      181 catgtattcg tcggggacgg tcaccgccac gtggtaaatc ggttccaaaa gcaccggttt
      241 gcactgcagg aaccccgacc ggaaggccat cgacccggcc accttgaagg cgatgtccga
      301 ggaatcgacc gtatggtacg acccgtcaaa gatcgtcacc ttcacgtcga ccacccggta
      361 gccggccagt cccccttcct gcatcgcttc caccactccc ttttccaccg atggaatata
      421 cttcccgggc accgcgccgc ctacgatttc atcctcgaac tcaaagccgg tgccgcgttt
      481 cagcggctcg atccggagcc agcagtcccc gtactgtccg tggccgccgg tctgcttctt
      541 gtatttcccc tgcacttcgg ttttgctctt gatcgtctcc cggtacggaa tgcgcggttt
      601 ttccagttcg acttccacgc cgtacttgcg cttgagccgg tcgatgatca cttctaagtg
      661 cagttccccc tgtccgtgaa cgatcgtctg cttgatgtcg ctgttcactt ccaccgaaaa
      721 ggtgggatct tcctcatgca ggcgcgccag gccggaggcg attttttcgt cgtcggtttt
      781 cgctttgggt ttgatcgcca tattgatgac cggtttggga aaatcgaccg gcggcaaaag
      841 gatcggcgcc ttgcggtcgc agagggtgtc gcccgtgtgg gtgtttttca gtttcacgaa
      901 cgcaccgatg tcgcccgccg acacggtgcc gacttctttg cgctccttgc cgttcatggc
      961 gtaaatctgg cccacccgtt cgctgtgctg gcgggtggcg ttgaaaatgt cgtcgccggt
     1021 tttcacgtgg ccggagaaga cccggaaaaa cgacagttcc cccacgtgcg gctcggaaat
     1081 cgttttgaaa atcaaggcgc agaccggctc gctgggcaga gttttgcgga cgacttcctg
     1141 ttccgtgccg gataaaattc ccttgacttc gggcacgtcc accgggctcg gagcaaagtt
     1201 ggccaggaaa tcgaccaaaa ggctcgtacc cgccgaagcc gtcgccgcgc cgactaaaac
     1261 cggaaaaatc ttcttagccg ccagaccctt cttcaacccg tactgaattt gcacgtcggt
     1321 cagttcgccg gcttcaaaga attgctctaa gagcgaatcg tccgcttccg cagccgcttc
     1381 gaggagtttc tgtcgcgcgt cggccgctgc atttttgaac tcggcgggaa tttcctccgc
     1441 tttggccgtt ccgccggtga accggaaggc tttgttctgc aggaggtcga ttactccgcc
     1501 aaatcccggc ccgctgccca aaggcaattg caccggcacg acgagatgcc cgaaccgctc
     1561 ctgcaaaacc gctaacactt tggcgaaatc ggcatgctcc ttgtcaattt tgttgacgaa
     1621 gatgacccgg ctcgtgccgt agcggtcgcc caactgccag acctgctcgg tgcccacttc
     1681 cacgccggag gcggcattca ccgccaccac cgcggtctcg cagacccgga gcccaccgtg
     1741 cacttccccg atgaaatcgg tgtaccccgg caggtccacg acgttgaact tggtcccgtt
     1801 ccaatcgaag tggagcaggg tggaactgat cgaaatcttc cgcgtgattt cgtccggact
     1861 gtagtcggaa atggtcgtcc cgtcgtcgac ttttcccaaa cgcgtggtaa ccttggcggc
     1921 aaataaggcg gcctcggcca gcgaggtctt ccccgacccg ccgtggccta cgaaagcgac
     1981 gttccgaatc tgctcaagcg tgtactcccg catcgctgca acaccccgga taaaacttgc
     2041 ctaacctacg tttcgtgcag gactttgtca actgtccggt aattgaagac ccggatcctc
     2101 cgctgcttga caccgccctc cccgtctcta cattagccgc acgatgcccc ctgcctcaaa
     2161 tacctcccgg agaggaaaac gcgacttcct caccgtcacc gacttttccg cccgtgagat
     2221 tagcgagaca ttgatgttag cccagtcgct taaaaagggc aagactcgtc cccagccctt
     2281 gaaagggaag agtgtcgcct gcatcttcca caaaccgtcg ttgcgcaccc gggtcagttt
     2341 tgagacggga atcttccagt tgggcggcca tccgatttac attacggaaa aagagattga
     2401 actcggccgg cgcgagacga ttgccgacgc cgcccgcgtc ttctcccgct atgtagcgtt
     2461 gattgtgatt cgcaccttcg cgcagaaaga tgtcgaggaa ctggccaaat acgcgacggt
     2521 ccctgtcatc aatgccttga ccgacctttc ccacccctgc cagattatgg gcgacctctt
     2581 caccatttgg gagcgccgaa agaatttgaa agcgctttcg gtcgcttatc tcggtgacgg
     2641 aaacaatgta gcgaattcct ggttggaatt ggcggcccga attccgctcg atttacggat
     2701 cggcacctcc ccgcagacga tgccggatga ggaaacgttc cggcgagcac aggagaccgg
     2761 gatttccaag atacgtatca cggctgaccc tttctccgcc gttcagggtg tcgatgtggt
     2821 ctacaccgac gtctgggcca gcatgggcca gaagcattta gccgaagaaa aagcccaatt
     2881 cctctcgcgc tttcaggtga attccgccct gctcaagcac gccaaagcga atgctttggt
     2941 catgcactgc ctgccggcca agcgcggtca ggagattacc gatgaggtga tggacggccc
     3001 gcagtcggtc gtcttcgatc aggctgaaaa ccgcctccac atccaaaagg cgattatggt
     3061 gaaattattg cacggctcga agatgtgatt tgttttctgg ctactggcta cttgaccctg
     3121 gctacttttt tccggccccc ggttaccccg tctttggcgg gacctgcgct ccgcttcggc
     3181 tggcttctgg tatttgggct tgtaactggc tactggctac ttgaccctgg ctactgactt
     3241 tatgctgccc gagattgtaa aatactctga ctgcttcgtc tgcggcgaca agaacccgat
     3301 cggcctcaag gttaagttct ttgccaaggg cgacaccgcc gtcgccgagt ataccgtcgg
     3361 tgacctgtac gaggggtata aagggctctt ccatggggga ctgattgccg cgctgttgga
     3421 tgaaattatg attaaagccg tcctg
//
LOCUS       DBNGZA010000462         7248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1575266, whole genome shotgun sequence.
ACCESSION   DBNGZA010000462 DBNGZA010000000
VERSION     DBNGZA010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1575266"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..489
                     /locus_tag="AC16FU_09870"
     CDS             76..489
                     /locus_tag="AC16FU_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR2994714.1"
                     /translation="MASVNKVIQIGNLGRDPELRYTPGGQPVATFTLATTDRWKDKEG
                     QLQDRTDWHNIVVWGKTAENTKEYLKKGRQVYVEGRIQTRSYDDRDGNKRYITEIVAQ
                     RVQFLGRREESAAGGPAVAEAEPEPQAAVDEDVPF"
     gene            526..2388
                     /locus_tag="AC16FU_09875"
     CDS             526..2388
                     /locus_tag="AC16FU_09875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR2994715.1"
                     /translation="MPDADFGVTARDVPRFFESGRKTIALLIGGAISVFLPFGIYLVT
                     LAAVVGFTDSGELAVVSRTLGIAHPTGYPLYTLVTHLFTWLPVGSTISRTGLFSALVA
                     SATNGLLYLCVLELFAMLGAGLRSNFRSVAIATGTALSLAFTALFWTQGNTNEVYALH
                     LFFISLATLQFLKWQKTVREEGVGTPDAQRLFLLLAFASGLGFTNHMTIGVLMPALLL
                     GGWATVKRIPYRAPWFWVGLGLFLGALTLYLYLPVRSAQNPLFDWGRPTNLGRFFRHV
                     SAWQYRVWMFSGPESQIKENFSHFIALFRTEYLWFWLLPAGIGLVTLFRKNWPVGLFL
                     LLALAGNFSYNLNYHIPDIDSYFLPFVFWYLLLGGVGIAAIISWLLGKHALVLRGIGW
                     LGLAGTIFFPARAVLQNYPKANQKTYLFAYDYAQNFANGLLPDAVVLTSLWDHYAPWL
                     YLHYLEGRRTDVTFIDVELCRRSWYFAYLARADSTLFQHSATEIGYFLVELRKFEQNL
                     PYDPGTIDQAYYRMLSSFVFNNLDRPIYADVTLDRQIAPQFQRIPDGLLFRLRVPTEK
                     YAPSLARIYFRGQDDPEVYKDAQAQEYLAMAEEMKRLRAEYDAYSAAKSPPLVP"
     gene            complement(2475..2981)
                     /locus_tag="AC16FU_09880"
     CDS             complement(2475..2981)
                     /locus_tag="AC16FU_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994716.1"
                     /translation="MNKPARMLPGMVLEFWPAYAVPEAQGFYDLHEEVFELRVKTSQR
                     LAAILQARQLSLDLDEAGRLAYLEVTRPRGLWEVSARHQPPFEYCRAEASFGLLPVFL
                     PAPKFYTDLDRTCLCVEFSAQAGWRSVEVAKQVIVDIDRHYRLLRVWVLGIEEDFSFR
                     KRLAWSQK"
     gene            complement(2978..3787)
                     /gene="ybgF"
                     /locus_tag="AC16FU_09885"
     CDS             complement(2978..3787)
                     /gene="ybgF"
                     /locus_tag="AC16FU_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR2994717.1"
                     /translation="MKGRRKESLHRNAAIKIVWVLAGVLLVSGCVSRSEIKRMMDQLD
                     YLESSNQKLERQAALSDSLLRVQEQEMERLKAGFNATNRDLLARLDNLDGKVSDMGEW
                     LSQITKRVERPMPKPVIPPIPPVDTTSPGAADTTKPSGTTVDPNQLYDTAFRDFRKQN
                     YDLAVSGFTEFLEMFPTSAKAADAQYWLAESCNKKGNYAKAIEEFTKVTFTYPQSDKA
                     PTALFKIGLAWEAQNQPAEAQKAYQQLVSQYPNSEAARFARDKLPAPKKSR"
     gene            complement(3899..4414)
                     /gene="pal"
                     /locus_tag="AC16FU_09890"
     CDS             complement(3899..4414)
                     /gene="pal"
                     /locus_tag="AC16FU_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR2994718.1"
                     /translation="MRKLTAILLMLSLAASGLMVGCAKKAPPPPPPAPAPTPTPAPPP
                     TPPPAPPPEEPKPKPEIKFQEIFFDFDKSFIREDAKPALEANAGILKDNANVTIRIEG
                     NCDERGTVEYNLALGQRRAEAAKKYLVDLGIEASRIQTISYGKERPRNPGHNEAAWQE
                     NRRDDFVIVSQ"
     gene            complement(4818..6146)
                     /gene="tolB"
                     /locus_tag="AC16FU_09895"
     CDS             complement(4818..6146)
                     /gene="tolB"
                     /locus_tag="AC16FU_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR2994719.1"
                     /translation="MKILRSICFGLLCLGVFSASSVAQQELRLRLRVPGFKPIEVGVD
                     DFASDSRIFGPGDQELLTKIPQIIRDDLWYALFFVVIEPDTAYLRTFQKEKLEPADWF
                     ALGAKFYLSGKLNFKADRITCDFELRDVNTNHKIVSDDVSQARGQERRIAHKIADAIV
                     KQFTGKDGIFSTQLAFVSAQSKNREIFVCDFDGANVRKVTDDRSINLSPSFSPDGTKL
                     LYTSYKAGNPDLWLLDLRSARSKKISAKAGLNSAAVWSPDGRSIAATLSYQGNPELYL
                     LDANGNIIRRLTQNEAIDSSPSFSPDASQVAFVSDRSGAPQIYLADVETGAVNRLTFA
                     GSYNDSPAWSPDGTQVALVMRNDQGNFDVCTVDVTGQNYRQLTSNGSNENPHWSPDGL
                     HLVFSSNRSGIFQIYTMDFDGNNQRMITSRNESNPTGGQNYNPTWGPYLK"
     gene            complement(6154..6771)
                     /locus_tag="AC16FU_09900"
     CDS             complement(6154..6771)
                     /locus_tag="AC16FU_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR2994720.1"
                     /translation="MKGEFVVSALAHLLLCVILFTGGTSRPLIVARPQPIQVRLVSLP
                     PEPPEAVAEAKPIKKPPVKETKGITFAEIKKPKKKAEKKAAAKVEEPKPQPQVSPSQT
                     GSRFGEAVVDAPDFAYSYYVDLILNKIYDGWANPVSSDFALSVVIYFKIRRGGQLADV
                     KVEAPSGIAAFDRAAYRAVVNAGPLPPLPAEYEGTELGIHLEFQQ"
     gene            complement(6768..7187)
                     /locus_tag="AC16FU_09905"
     CDS             complement(6768..7187)
                     /locus_tag="AC16FU_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR2994721.1"
                     /translation="MELSENRRYHPRTPLADINVTNLVDVVLVLLIIFMITAPMLQSG
                     IDINLPKAKTEAKEVSESATVTVTRERGIFINDKFVRPDRFETQLGVLKRSGQVRSIL
                     LRADKDVPYGFVVETMAKIRAAGIENLNLITVPEERK"
BASE COUNT         1356 a         2211 c         2032 g         1649 t
ORIGIN      
        1 tccgcaaagc ccgggcgcgg aaggcagacc tcatcatttt ccccgaagtt tagaaacgtt
       61 gataaaggag tccccatggc gagcgtcaat aaggtcattc agatcggcaa cctcgggcgc
      121 gatcccgaac tgcgctacac ccccggcggc cagcccgtcg ccaccttcac tctggcgacc
      181 accgaccgct ggaaggacaa ggagggtcag ctgcaggacc gcaccgactg gcacaacatc
      241 gtcgtctggg gcaaaactgc ggagaatacc aaagagtacc tcaagaaggg ccgtcaggtc
      301 tatgtcgaag gccgcatcca gacccgctcc tatgacgacc gcgacggcaa caagcgctac
      361 atcaccgaga tcgtcgccca gcgcgtgcaa tttctcggcc ggcgtgaaga atccgccgcg
      421 ggcggaccgg cagttgccga agctgagccc gaaccgcaag ccgcagtaga cgaggacgtt
      481 cccttttaat cggctcggta tgagccatat cgaccagagg cggtcttgcc cgacgcagat
      541 tttggggtta cggccaggga cgtgccgcgt tttttcgaaa gcggccgaaa gaccatcgca
      601 cttctgatcg ggggtgcgat ttccgttttt ctcccgttcg gtatttacct tgtaactctg
      661 gccgccgtgg tcggcttcac cgattcgggc gaactggcgg tggtgagccg aactctgggc
      721 atcgcccacc ccaccggcta ccccctctac acgctcgtca cccatctgtt cacctggctt
      781 ccggtcggga gcaccatcag ccgcacgggc cttttctcgg ctctggtcgc atctgcgacc
      841 aacgggcttc tctacctttg cgttctggaa ctttttgcga tgttgggggc gggcttgcgc
      901 tcaaacttcc gctcagtcgc gattgcgacc gggactgccc tttccctggc tttcaccgcc
      961 ctcttttgga cgcaagggaa taccaacgag gtgtatgccc tccacctctt tttcatttct
     1021 ctcgccaccc ttcaatttct caagtggcaa aaaactgtcc gggaggaggg tgtgggaacg
     1081 cctgacgctc agcggctctt cctgcttctg gcctttgcct ctggcctggg cttcacgaac
     1141 catatgacca ttggggtact gatgccggcc ttgctgctcg gcggctgggc aaccgtgaag
     1201 cgcattcctt accgagctcc ctggttctgg gtcggattgg gcttgttcct gggagcatta
     1261 actttgtatc tctacctacc cgtccgctcc gcccagaacc cgctcttcga ctggggccgt
     1321 ccgacaaacc ttggaagatt ttttcgccac gtttccgcct ggcaataccg cgtctggatg
     1381 ttctccggcc cggagtcaca gataaaggag aacttttccc actttatcgc cctgttccgc
     1441 accgaatacc tgtggttctg gcttttgcct gccgggatcg gcctggtcac gctcttccgg
     1501 aaaaactggc cggtgggact tttcctctta ttggctttgg ccggcaattt ctcctacaat
     1561 ctaaactacc acatccccga tatcgattcc tacttcctgc cctttgtttt ctggtacctc
     1621 ttgctcggcg gtgtgggaat cgccgccatc atcagctggc ttttgggcaa acatgccctt
     1681 gtgctacggg gtataggttg gctcggcctg gccggcacca tctttttccc ggcgcgtgcc
     1741 gtcctgcaga attatcccaa ggcaaaccaa aaaacctacc tcttcgccta cgactatgcc
     1801 cagaatttcg ccaacggtct gctccccgat gccgtggtgt tgaccagcct ctgggaccac
     1861 tatgctccct ggctctatct ccattacctt gaaggccgcc gcacggatgt taccttcatc
     1921 gatgtcgaac tctgccgccg ctcctggtac ttcgcctatc tggcgcgcgc cgattcgacc
     1981 ctctttcagc attcagctac ggagattggt tactttttag tcgaactccg gaaattcgag
     2041 cagaacctcc cctacgatcc agggacgatc gaccaggcct actaccggat gctttcctca
     2101 tttgtgttca ataacctcga ccgtccgatc tacgcggatg taacgctcga ccgccagatt
     2161 gctccccagt tccagcgcat tccggacggg ctgctcttcc gcctgcgcgt ccccaccgag
     2221 aagtacgcac cgagcctggc gcgaatctac ttccgtggcc aagatgatcc ggaggtgtac
     2281 aaggatgccc aggcgcaaga gtacctggcc atggcggagg agatgaaacg cttgcgcgcc
     2341 gagtacgacg cctactccgc cgctaagagt ccacccctgg taccgtgagc aggggtttga
     2401 ttaatccaac ctctacaatc ggtctaaggg cgctcagccg ggctgccctt tttcccggct
     2461 gatttggaga atttttactt ctggctccag gccagccgct tgcggaagga gaaatcttct
     2521 tcgatcccca aaacccacac ccgcaagagc cggtagtgcc ggtcgatgtc gacgatgacc
     2581 tgcttggcca cttccacgct gcgccagccc gcctgcgcgg aaaactcaac gcaaagacag
     2641 gtacggtcga ggtcggtgta aaacttgggg gcgggcaaaa agaccggcag aaggccgaac
     2701 gacgcctcgg cccggcagta ctcaaaaggc ggctggtggc gggcggaaac ttcccacagc
     2761 cctcgcggcc gggtcacttc taaatacgcc aggcgccccg cttcgtccag atcgagggag
     2821 agctgccgcg cctgcagaat ggccgcgagc ctctgggagg ttttgacccg gagctcaaag
     2881 acttcctcgt gcaggtcgta aaatccctgc gcttcgggca cggcgtaggc cggccagaac
     2941 tccagcacca tcccggggag cattctcgcc ggcttattca tcggcttttc ttcggagccg
     3001 gcagtttatc acgggcaaaa cgcgccgctt cggaattcgg gtactggctc accagttgct
     3061 ggtacgcttt ctgcgcttcc gccggctggt tctgagcttc ccaggccagg ccgattttga
     3121 acagcgccgt gggcgccttg tcgctctggg ggtaggtaaa ggtcaccttg gtgaattctt
     3181 caatcgcttt cgcgtagttt cccttcttgt tgcaggactc cgccagccag tactgggcgt
     3241 ccgccgcttt ggcgctggtg ggaaacatct ccagaaattc ggtgaatcca gagacggcta
     3301 aatcgtaatt ctgcttgcga aaatcccgga acgccgtgtc gtagagttga ttgggatcga
     3361 ccgttgtccc ggaaggtttg gtggtatcgg cggcgcccgg agaggtcgta tccaccggcg
     3421 ggatgggggg aatgaccggc ttgggcatcg gcctttccac cctttttgtt atttgggaga
     3481 gccattcgcc catgtcgctg accttaccat ccaagttatc caggcgcgcc aagaggtcgc
     3541 gatttgtggc gttgaaaccg gccttcaagc gctccatctc ctgctcttgg acccgcagaa
     3601 gactgtcgct taaagctgcc tgccgttcaa gtttctggtt gctcgactcc aggtagtcca
     3661 gctgatccat catccgcttg atttccgagc gggagacgca gccgctgacc aatagcactc
     3721 cggccagcac ccagacgatc tttatcgcag cgttgcggtg caaagattcc ttccttctcc
     3781 ctttcatacc ttctacctga aaataacccc ctgatcccat tttggcgaga ccaaggggtt
     3841 cttccctacc acctacttgt ggtgagcctg ccgaaccacg acctgccacc ttccaccttt
     3901 actggctgac aatgacgaaa tcgtcgcgcc ggttttcctg ccaggccgct tcattgtgtc
     3961 ccgggttgcg cggccgctcc ttgccgtaac tgattgtctg gatacgggag gcctcgatcc
     4021 ccaaatcgac taaatacttc ttggccgcct cggcccggcg ctggcccagg gctaagttgt
     4081 attccaccgt gccccgctcg tcgcagttcc cttcaatccg gatggtgacg ttcgcattgt
     4141 ctttgagtat ccccgcattc gcctccaaag ccggcttggc atcctcccgg atgaacgact
     4201 tgtcgaagtc aaagaagatt tcctgaaact tgatttccgg cttcggcttc ggctcttccg
     4261 gaggcggcgc cggcggcggc gtgggtggcg gtgccggtgt tggcgttggg gccggtgccg
     4321 gcggtggtgg tggcggcgct ttcttggcgc agccgaccat aagcccgctg gctgccaagc
     4381 tgagcatgag caaaatcgct gtcagtttcc gcatcgggtt cctccttgat ttttcgcgtt
     4441 caaattccct cgcttgactc ataataaacc acgccgctcg tggctgtcaa cagaaaaatg
     4501 ctcgcccgca tcgctgtagc gcaggttggt tcgccctgag cgaagccgaa gggtttactt
     4561 gtgccgagtc cgcctcaggt ggatcgaggc atggaacctg cgccattttt tattcgcccc
     4621 ggtagcacag gcccttgtgg ccgttccgag gctggtctga tacgactcta cgagaagcct
     4681 cggctatgga ggcaagaaat gtcttttatc cctcggtcag gcttctcggt gagagtcgtg
     4741 ccgaccagcc tgacccgaat tctgatttct gtacgattcc ccccaaatcc cgactccctt
     4801 atcccaaatc cgctttttta tttcaagtac ggcccccacg tgggattgta attctgcccc
     4861 ccggtcggat tcgactcgtt ccgagaggtg atcatccgct ggttgttgcc gtcaaaatcc
     4921 atcgtgtaaa tctgaaagat gccgctccgg ttggaactga agacgaggtg caatccgtcg
     4981 ggcgaccagt gcgggttttc gttcgagccg ttggaggtga gctgccggta attctgtccg
     5041 gtgacgtcaa cggtgcaaac atcaaaattc ccctgatcgt tgcgcatcac cagcgccacc
     5101 tgcgtgccgt cgggcgacca ggccggcgaa tcattgtaac tgcctgcgaa agtcagacgg
     5161 ttgaccgcgc cggtttccac gtcggctaag taaatctgcg gcgcgccgga gcggtccgac
     5221 acgaaggcca cctgactggc gtccggcgaa aacgaaggtg aggaatcaat cgcctcattc
     5281 tgcgtcaggc ggcggatgat gttgccattg gcgtccaaaa ggtacagttc cggattcccc
     5341 tggtaactca aggtcgcggc gatggagcgg ccgtccggcg accagaccgc cgcgctgttc
     5401 aaccccgctt tggccgagat ttttttcgag cgagccgacc gcaagtccag aagccacagg
     5461 tctgggttgc ccgctttgta cgaggtgtaa agtaacttcg tcccgtccgg cgaaaaggac
     5521 ggtgacaggt tgatcgaacg gtcatcggtg actttgcgga cgttggcgcc gtcaaaatcg
     5581 cagacaaaaa tctcccggtt cttcgactgc gccgaaacga aggcgagctg cgtggagaaa
     5641 attccgtctt tgccggtgaa ctgcttgacg atggcgtcgg cgatcttatg cgcgatccgc
     5701 cgctcctgcc cccgcgcctg cgacacgtcg tcggagacga tcttgtgatt ggtgttcacg
     5761 tcccggagtt caaagtcgca ggtaatccgg tcggctttga agttcagttt accggagagg
     5821 tagaatttcg cgcctaaggc gaaccaatcc gccggctcca gtttttcttt ttggaaggtc
     5881 cgcaggtaag cggtgtccgg ttcgatcacg acgaagaaga gcgcgtacca gaggtcgtcc
     5941 cggatgatct ggggaatttt ggtcagaagt tcctggtccc ccgggccgaa gatgcgcgaa
     6001 tcggaggcaa aatcgtccac gccgacctca atcggcttga atcccggcac ccgcaggcgc
     6061 agccgcaact cctgctgcgc tacagacgag gcggaaaaga ccccgagaca gaggagaccg
     6121 aaacatatgc tcctgaggat tttcatcaga tcgctactgc tgaaattcga ggtgaatgcc
     6181 cagctccgtg ccttcgtact ctgccggcaa aggcgggagc ggcccggcgt tgaccaccgc
     6241 ccggtaggcg gcccggtcga acgccgcgat gcccgagggc gcttccacct tgacgtcggc
     6301 cagctggccg ccgcgccgaa ttttgaagta gatgacgacc gaaagggcaa agtcgctgga
     6361 gaccggattg gcccagccgt cgtaaatttt gttcaagatg agatcgacgt agtaggaata
     6421 ggcgaaatcg ggggcatcca ccaccgcttc gccgaaacgt gagccggttt gacttggact
     6481 gacctgcggt tggggcttcg gttcttcaac tttggctgcg gctttcttct ccgctttttt
     6541 ctttggtttc tttatttcgg caaaggtgat ccctttggtt tcctttaccg gcggcttttt
     6601 gatcggcttg gcctccgcga ccgcttccgg cggttcgggt ggcagagaga ccagccgcac
     6661 ctggatcggc tgcggccggg cgacgatcaa gggccgggat gtgccgccgg tgaacagaat
     6721 gacgcagagt aaaagatgcg ccagagcaga aacgacgaac tcccctttca cttccgctcc
     6781 tccggcaccg tgatcaggtt cagattttcg atccccgccg cccggatttt ggccatcgtc
     6841 tccaccacaa agccgtacgg cacgtccttg tccgcccgca aaagaatcga acgcacctgg
     6901 ccgctgcgct tgagcacgcc cagctgcgtt tcaaaacggt cgggccgcac gaacttgtcg
     6961 ttgatgaata ttccccgctc gcgggtgacg gtcaccgtcg ccgattcgga cacctctttg
     7021 gcttcggttt tggctttggg gagattaatg tcgatcccgc tctggagcat cggcgcggtg
     7081 atcatgaaga tgatcaaaag caccaagacg acatcgacca gattggtgac gttgatgtcg
     7141 gctaagggcg tccgcgggtg ataccttcta ttttcgctta attccacgtc aaatccatcc
     7201 tgtcagtctt gccgtaaggt gatgacgggt cattctcttg taggggct
//
LOCUS       DBNGZA010000463         2874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_196939, whole genome shotgun sequence.
ACCESSION   DBNGZA010000463 DBNGZA010000000
VERSION     DBNGZA010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_196939"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2874)
                     /locus_tag="AC16FU_09910"
     CDS             complement(<1..>2874)
                     /locus_tag="AC16FU_09910"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR2994722.1"
                     /translation="SKGGNSIRVNSFPSMAVDRSSGPRRGWIYIVYPEKNPVSPDIFL
                     VRSTDGGSTWSTARKVNQDNSGKDQWFSWVSVDPVTGILYVAYYDSRNFAANDSAQVF
                     LTASLDGGDTFADLLVSDVPFLPTPIPGLAAGYMGDYIGVSSVNGVVWPCWNDNRTGI
                     HQAWTSRIVFITVGSPPQISVSPETLDYGNVFLAYPETLSISIRNLGFPDTLFVTNIQ
                     SDNSDFTPELTSLALPGGGSRAVKVAFNPSTAGLVNATLSIASNDTANPTVTVALRAN
                     ALIPPDISVSPDSLEADLLTGASAVETVTVTNSGGSDLDFDIAVAGPGGAAAKVNFIA
                     NQAIRSAKVLATGKPVPIEQFTRLRSQFQREISVAADNVVDKNQPSQKNREQRGALAK
                     AAGVEIFGSPANSFFAGPRTRGNLFTCTVSNTLIEHRFYMNPPAGNQLWFLVYEGAAQ
                     VGTYNLVSASDVTPAGPGEGWYSSGSINVPLVAGRFYLIVASFEQASNYFNEQGISPY
                     PIPASFGELTAGAGWDWAPTSNFPPDPVQNVPAFAFGSPVAYYQALVTGVGTSWLSAN
                     PDSGTVPAGESLDVAVTFDATGLNGGDYYADVLINSNDPDRPQDTVFAHLHVTGAPDI
                     AVSDTALDFGSIFTGTAKTETLVVTNPGTDSLAINNITSDNAHFTVNITAFFLNPGDS
                     QNVLVTFATNDTGLFTGNLTITSNDPDEPTVNVSLRGRGLLPPDILVTPDSLAADLFT
                     GEIAVETLTVANTGYSDLNFTISIKDTGATAQAVKVRPTGISRLSGGSGALTGTKADL
                     SPFQREISADVLAQTGSPGSVPKRSPGKISSSALQATTLLLTTTSVTGSVGQALTNLG
                     VAYDLLQTDVFTGIDFSPYTTIVVAMDGGLVEEPDVQALATAAASGKKLIMIGGTNYP
                     PYYNGMQLYLLSHTNQQGWTISSPPHLIVTNPSHPLAAGLPSTY"
BASE COUNT          603 a          784 c          920 g          567 t
ORIGIN      
        1 ataggtggac ggcaagccgg ccgccagggg gtgggacgga ttggtaacga tgagatgagg
       61 tggggacgaa atagtccacc cctgctggtt agtatgagac aatagataca gctgcatgcc
      121 gttgtagtag gggggatagt tcgtcccgcc gatcatgatc agctttttcc cgctggcagc
      181 ggcagtggcc agggcctgga catcaggttc ctcgaccaga ccgccgtcca tagcgacgac
      241 aatcgtcgtg tagggtgaaa agtcaatccc ggtgaaaacg tcggtctgga gcaaatcgta
      301 ggcgacgccc aaattggtca gcgcctgccc caccgatcca gtgaccgacg ttgtcgtcaa
      361 gagcagagtc gtcgcctgaa gcgccgaact tgaaattttg cccggcgatc ttttcggcac
      421 gctcccgggc gaaccggtct gcgccagcac gtcggcggag atctcccgct ggaacgggga
      481 caaatcggct ttcgtcccgg tcagtgctcc gcttcctccg gagagccgag aaatgccggt
      541 gggtctgacc ttcaccgcct gtgccgtcgc gccggtatct ttgatggaaa tagtgaagtt
      601 caaatcgctg taaccggtgt tggcaacggt gagcgtttca accgcgattt cgccggtgaa
      661 gaggtcggcg gccagcgaat cgggcgtcac cagtatgtcc ggcgggagta ggccccggcc
      721 gcgcaaggag acgttaaccg tcggctcatc cgggtcgttg ctcgtaatgg tgagattgcc
      781 ggtgaaaagc cccgtgtcgt tggtcgcaaa ggtgaccaga acgttctggc tatccccggg
      841 attcaagaag aacgcggtga tattgaccgt gaaatgcgcg ttatccgagg taatgttatt
      901 gatggccaaa ctgtcggttc ccggattggt aaccacaagc gtttcggttt tggcggttcc
      961 ggtgaagatg gagccgaagt ccaaggcagt atcggagacg gcgatatcgg gcgcgccggt
     1021 gacgtggagg tgggcgaaaa ccgtgtcctg cggcctgtcc ggatcgttgc tgttgatgag
     1081 gacgtcggcg tagtagtcgc cgccgtttaa gccggtggcg tcaaaagtca ccgccacatc
     1141 cagagactcg ccagccggaa cggtgccgga atcgggattg gccgatagcc aggaggttcc
     1201 taccccggtc acaagggcct gatagtacgc caccggagac ccgaaagcga aagccggaac
     1261 gttttgcact ggatcgggcg ggaagttcga cgtgggtgcc cagtcccagc cggcccccgc
     1321 ggttaattcg ccgaaagagg cgggaatggg gtagggggag atgccctgct cattaaagta
     1381 gttgctcgcc tgttcgaaag atgcgacgat gaggtagaat cttccagcca ccaaaggcac
     1441 gttgattgaa ccagaactat accacccctc acccgggccg gcgggagtga cgtcggatgc
     1501 gctgactaaa ttgtacgttc ccacctgggc ggcgccttcg tacaccaaaa accagagttg
     1561 attgcccgcc ggcggattca tgtaaaaccg gtgttcgatc aaggtgttgg acaccgtgca
     1621 ggtgaagagg ttgccccggg ttcggggtcc ggcaaagaaa gagttcgccg ggcttccaaa
     1681 tatttcaact ccggcggctt tcgccagagc gcctctctgt tctcgatttt tctgagaggg
     1741 ttggttttta tcgacgacgt tatcggctgc aacggaaatt tcccgttgaa actgagatct
     1801 cagccgggtg aactgttcta tcgggaccgg cttaccggtc gccagcactt tggccgaccg
     1861 gatcgcttgg tttgcaatga agttgacctt cgcggccgcc ccgccgggtc ctgccacagc
     1921 aatgtcaaag tccagatcgc tcccgccgga gttcgtaacc gtcaccgtct ccaccgccga
     1981 ggcgccggtc aaaagatcgg cctccagtga atcaggtgag acggaaatgt ctggcggaat
     2041 caacgcgttg gcccgcaggg ccaccgtcac ggttggatta gcggtatcgt tgctggcgat
     2101 ggagagcgtg gcgttgacga ggcccgcagt cgagggattg aaggcgactt tcaccgcgcg
     2161 actccccccg ccggggagag ccagagaggt caactccgga gtgaagtcgg aattgtcgga
     2221 ctggatattc gttacaaaaa gcgtatcggg aaagcccagg ttacggatcg atatgctcaa
     2281 ggtttccggg taggccagga agacattccc gtagtcaagc gtctcgggcg aaacgctgat
     2341 ctgcggggga ctgccgacgg tgatgaaaac gatgcgggag gtccaggcct ggtggatgcc
     2401 cgttcggttg tcgttccaac agggccagac caccccgttg actgaggaaa ctccaatgta
     2461 atcccccatg taaccggccg ccaggccggg aatcggggtc gggaggaagg gcacgtcgct
     2521 gaccaggagg tcagcaaacg tatcgccgcc atccaaagag gcggtgagaa agacctgcgc
     2581 cgagtcgttg gccgcgaaat tgcgactgtc atagtaggcc acgtagagaa tacccgtcac
     2641 cggatcgacg gaaacccagg aaaaccactg gtctttgccg ctattatcct gattcacttt
     2701 ccgcgcggtg ctccaggtgc tgccgccgtc ggtggagcgc accaggaaga tatccggaga
     2761 gaccggattt ttctccgggt agacgatgta aatccaccct cgccgggggc cactgctgcg
     2821 gtcgaccgcc atggagggaa agctgttgac ccggatggaa ttgccccctt tgct
//
LOCUS       DBNGZA010000464         7315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2006002, whole genome shotgun sequence.
ACCESSION   DBNGZA010000464 DBNGZA010000000
VERSION     DBNGZA010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2006002"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..95
                     /locus_tag="AC16FU_09915"
     CDS             <1..95
                     /locus_tag="AC16FU_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994723.1"
                     /translation="KRRSKKYGIASLCIGGGQGIAAVFERDEAR"
     gene            169..987
                     /locus_tag="AC16FU_09920"
     CDS             169..987
                     /locus_tag="AC16FU_09920"
                     /inference="COORDINATES: protein motif:HMM:NF023385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HIR2994724.1"
                     /translation="MTTHAFTVDVEDWPQSTLHSHHVITDRVVQNTRRLLHILCEHNI
                     HGTFFVLGIVAETFPRIVTEIAQEDHEVGTHGWSHVPIFTQSAREFARDLKKSLATLE
                     SILGQKVIGHRAPIFSITANTFWAFQVMADCGIRYDTSVFPIRGFRYGIPEARRFPYE
                     ISTDNSHQLTIFPLTTFRVLRKNLPACGGGYFRLFPYAYTRLAFRQVEGLGQPVMFYT
                     HPHELDVEESKLYRSQTPFKKRFTQFTGRRGMEQKLHRLLSEFCFAPARKVLQV"
     gene            984..1646
                     /locus_tag="AC16FU_09925"
     CDS             984..1646
                     /locus_tag="AC16FU_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR2994725.1"
                     /translation="MRGAARVQRLFDRTAEKFDTFYAEKKSPFKRFIDQHFHRDIFER
                     FELTFQHAQPIGRKTVLDVGCGSGLYCIEFARRGARRVVGIDFAPKMLELARAAAREQ
                     KVGDLCKFIQAEFLAYSFREQFDYTVALGVFDYLREPIPFLQKMRDVTREKILISVPS
                     VHWLRTPIRKFRYLFKRAPVVLYSRRKLEKMLREAGCTTFEIIKIPGAGMDFFVAINP
                     CG"
     gene            1793..3901
                     /gene="acs"
                     /locus_tag="AC16FU_09930"
     CDS             1793..3901
                     /gene="acs"
                     /locus_tag="AC16FU_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase alpha subunit"
                     /protein_id="HIR2994726.1"
                     /translation="MPHPLDYIFRPRSVAVIGASTQKGTLGREILHNIIEYEFNGKVF
                     PVNPRATVIHSIKCYSTILDVPDAVDLAVIAVPRDAVLPVVDQCGQKGVKGLVVITAG
                     FKETGPKGAELERQLGGKVADYGMRMIGPNCFGVINTEPEVRLNATFSKTKPLGGTIA
                     FESQSGAMGEAILYHAQQINLGFSMFASVGNKTNVSGNDLLQYWRDDPNTKMILLYLE
                     NFGNPRKFTQIARQLTRKKPIIAVKSGRTMQGARAATSHTGALAGLDVGVDALLSQCG
                     VIRASSIEEMFDTARAMATQPFPRGKRVAIVTNAGGPGIMTTDACVNLGLEIVHLTPE
                     TKKKLREFLPEEASVENPVDMIASATAERYGKTLAAVLADPQVDAVIVLCVPPINVNA
                     VEVAQAIISVRKNFPDKPLLASFMGVAEHSPGTELLIANGIPAYTYPESMVKALADLL
                     KYRQWLARPEGQVKVFSVKKVQAEAIITEATSNGQKQLVGEPALNLLAAYGIPIAPYA
                     AAATPEEAVQLAAQVGFPVVLKLNSPKLLHKTEVGGVLVDLRGEKEVSEGFRTLEKRY
                     RQASGDGDFSGVVVQQMIKSGVETVIGMVSDPGFGPLMMFGLGGIYVEVLKDVAFKIH
                     PLTEADAWEMVKSLKSYPLLAGFRGAKPVDLEELVNAILKLSQLVSDFPQIDQFDINP
                     FIVSPRKDATRAVDARFLLR"
     gene            4022..4954
                     /locus_tag="AC16FU_09935"
     CDS             4022..4954
                     /locus_tag="AC16FU_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR2994727.1"
                     /translation="MPQQSAETIIQAEKLAKKYGDLVAVDGIDFGIRRGETFGFLGPN
                     GAGKTTTMKMIYCFMPPTWGKLSVAGYDVTRNPREIKALLGVVPQEDSLDPDLTVYGN
                     LLAYSRYYDISPKVAHERIVELLDFMQLTEKKNTIIYALSGGMKRRLVLARGLINSPQ
                     ILVLDEPTTGLDPQARHLVWQKLRHLKSQGLTILLTTHYMEEASLLCDRLVIMNAGKI
                     LDSGSPQELVKKVVGQEVLEIYSADDALFQKVRSSVGPRATAELFGDVIYLYSNDGQP
                     ILSELPRLGIENFLHRRATLEDVFLRLAGRTLKE"
     gene            complement(5064..5801)
                     /locus_tag="AC16FU_09940"
     CDS             complement(5064..5801)
                     /locus_tag="AC16FU_09940"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR2994728.1"
                     /translation="MDAQSQSTYTRREALTVLVGGAASAAALLTVGSTESLAQIAERS
                     QTMPDQITPKPLKPSLLQMDGISARTIEEHEKLYKGYVNKTNEIRGKLATVDLSTANQ
                     VYSDLRVLKLELTFAIGGVKNHEIYFDNLGGKGGQPSGDLMAMIKRDFGSFDSWQKDL
                     KATGISARGWAWLAYDHDDGKFFNYLGDAQNTFPIWNATPVVALDTYEHAYFIDYGVN
                     RAAYIDKFFANLDWPAIEKRFAGLKIK"
     gene            complement(6051..7202)
                     /locus_tag="AC16FU_09945"
     CDS             complement(6051..7202)
                     /locus_tag="AC16FU_09945"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR2994729.1"
                     /translation="MTFLRTLLCTKCGQEHDPSRPQNLCPCGGPLYAQYDLVSIAKKL
                     KKSDLPSRPASLWRYRELLPVEDERNVVTLGEGFTPLIKADQLGARLGLENLWIKDES
                     GNPTGSFKARGMAVAISKARELGITKVALPSAGNAGGAAAAYAAKAGIDCTVFVPDDT
                     PKAFVEECQACGAKVRLVSGSIADAGKEMAKEREKEGWFDLSTLKEPYRVEGKKTLGY
                     ELAEQFDWELPDWVIYPTGGGTGLVGMWKAFDELEKLGWIGKKRPKMVTVQASGCAPI
                     VKAFEEKAERTSPWPNPQTRALGLRVPSAVGDFLMLKVLRESNGAAVAVSEEEIEAGQ
                     KSLAQAEGIFACPETGATMAGLKKLVEQSRISQQDSVVVFHTGSGYKYL"
BASE COUNT         1533 a         2100 c         2101 g         1581 t
ORIGIN      
        1 tcaagcgccg gagtaaaaag tacggtatcg cctccctctg catcggcggc gggcagggca
       61 ttgcggcggt gtttgagcgc gatgaagcgc ggtaaagccg cagcgtgaca tggttcagtc
      121 ccaaaagcca ccttaaacag ttttgctttc gccagggggg tgtcaatcat gacgacgcac
      181 gccttcaccg tggatgtcga agattggccg cagtccacct tacattctca ccacgtcatc
      241 accgaccggg tggtgcaaaa tactagacgg ttattgcata ttctgtgtga acacaacatt
      301 cacggaactt tttttgttct gggcatcgtc gccgagacgt ttccccggat agtgaccgag
      361 atagcacagg aggaccacga agtgggcact cacggttgga gtcacgtgcc gatcttcacc
      421 caatcggcca gggaattcgc ccgagatttg aaaaagtcgc ttgccaccct cgaatctatt
      481 ctgggccaga aagtcatcgg gcaccgtgcg cctatttttt ctataactgc caatactttc
      541 tgggcttttc aggtaatggc cgactgcggt atccggtacg atacgagcgt tttccccata
      601 cgcggctttc ggtacggtat tccggaggcc aggcgttttc cctacgaaat ctcaacagac
      661 aacagccacc agctgaccat ctttccgctt accacctttc gggttctgag gaaaaacctc
      721 cccgcctgtg gggggggata tttccggctc tttccgtatg cgtacactcg gctggcgttc
      781 cgacaggttg aaggcctggg ccaaccggtg atgttctaca cccacccaca cgaacttgat
      841 gtcgaggagt caaaactcta ccgctcccaa acaccgttta agaagcgttt cacccaattc
      901 accggccgca ggggaatgga acaaaaactg caccgtttgc tctccgagtt ttgcttcgcc
      961 ccggcccgga aggtgctcca ggtatgaggg gcgcggcccg ggtccagcgg ctcttcgacc
     1021 gcaccgctga aaaattcgac accttctacg ccgaaaagaa aagccccttc aagcgattca
     1081 tcgaccagca cttccaccgc gacatcttcg agcgcttcga gctcaccttc cagcacgccc
     1141 agcctatcgg gagaaaaacg gtgctggatg tgggctgcgg ctccggcctc tactgcattg
     1201 agttcgcccg gcgcggcgcc cggcgcgtgg tgggtatcga tttcgcaccg aagatgcttg
     1261 aactggcacg ggccgcggct cgggagcaaa aggtgggcga cctttgcaag ttcatccagg
     1321 ccgagttcct tgcctactcg tttcgggagc aatttgatta taccgtggcg ctgggcgtgt
     1381 tcgattacct ccgggaaccg ataccgttct tgcagaaaat gcgggacgtt acgcgtgaaa
     1441 aaatcctgat ttccgtgccg tccgtccact ggttgcgcac cccgattcgc aagtttcgct
     1501 acctgttcaa acgggcgcca gtggtcctct acagccgcag gaaattggaa aagatgctgc
     1561 gcgaggcggg atgcacaacc tttgagatca tcaaaattcc cggagcggga atggattttt
     1621 ttgtggctat caatccctgc ggctaatttc tcttcatttg cgtcgctctg tgacttgcgg
     1681 aaagtttttg tagcgacggt ctcccgaccg tcgtgaatga cgatccctcc gctttcgagg
     1741 tttgttcggc gaactttctc tgcgtagatt cttttgaggg tcaatgaaag atatgcccca
     1801 tccgcttgac tacatcttcc gcccgcgctc ggtggccgtg atcggcgcct ccacccagaa
     1861 gggcaccctg ggccgggaaa tcttgcacaa catcatcgag tacgagttca acggcaaagt
     1921 cttcccggtc aatcccaggg ccacggtcat ccactccatc aagtgctact ccaccatcct
     1981 cgacgtcccg gacgcggttg acttggcggt tatcgccgtg ccccgggatg cggtgctgcc
     2041 ggttgtggat cagtgcgggc aaaagggggt caaggggctc gtggtcatca ccgccgggtt
     2101 caaggagacc ggacccaaag gagcggagtt ggaacggcag ctgggcggga aggtggcgga
     2161 ctatgggatg cggatgatcg ggccgaactg cttcggggtc atcaacaccg aaccggaggt
     2221 acggctgaac gccactttca gcaaaaccaa gccgctgggc ggtaccatcg cttttgagtc
     2281 ccagtcgggc gccatgggcg aggcaattct ctaccacgcc cagcagatca atctggggtt
     2341 ttcgatgttc gcctcggtgg ggaacaagac caacgtctcc ggcaacgacc tgttgcagta
     2401 ctggcgggac gatccgaata cgaaaatgat tcttctctac ctggagaact tcggcaaccc
     2461 gcgcaaattc acccagattg cccggcaact cacccgcaag aagccgatca tcgcggtcaa
     2521 atcgggccgg acaatgcagg gggcccgcgc ggccacctcg cacaccggcg cactggcggg
     2581 gctggacgtc ggcgtcgatg cgctcctctc ccagtgcggg gtcatccggg cttcctccat
     2641 cgaggagatg ttcgacaccg cccgggccat ggccacccag cccttccccc ggggcaagcg
     2701 ggtggcgatc gtcaccaacg ccggcggacc gggtattatg accaccgacg cctgcgtcaa
     2761 tctcggcttg gaaattgtcc acctgacgcc ggaaaccaaa aagaagctgc gcgagtttct
     2821 gccggaagag gcctcggtgg aaaatccggt cgatatgatc gcctcggcta cggcggaacg
     2881 ctacggcaaa acgctggctg ccgttctggc cgatccgcag gtcgatgccg tgatcgtcct
     2941 ctgcgtgccg ccgatcaacg tcaacgcggt ggaagtggca caggcgatca tttcagtcag
     3001 aaagaacttt cccgataagc cgcttttggc ctcgtttatg ggagtggcgg aacactcccc
     3061 cggcacggaa cttttgattg ccaacggcat cccggcctac acctatccgg agtcgatggt
     3121 caaggcctta gccgacctgc tcaagtaccg gcagtggctg gcgcggcccg aagggcaggt
     3181 gaaagttttc tccgtcaaaa aagtgcaggc ggaggcaatc atcaccgaag ccacctctaa
     3241 tggccagaag caattggtcg gggaaccggc gctcaatctt ttggcggcgt acgggatccc
     3301 catcgcgcct tacgcggcgg cggccacgcc cgaggaagcg gtgcagctcg cagcacaagt
     3361 gggtttcccg gtagttttga agctcaactc acccaaactc ctgcacaaga ccgaggtggg
     3421 cggcgtcctc gtagacctcc ggggcgaaaa ggaagtgagc gagggctttc gcacgttgga
     3481 aaaacggtac cgccaagcct ccggtgatgg tgatttctcc ggggtcgtgg tgcagcagat
     3541 gataaaatca ggcgtcgaga cggtaatcgg gatggtgagc gatccgggct tcggtcccct
     3601 catgatgttc ggcctgggcg gcatctacgt cgaggtgctc aaagacgttg ccttcaaaat
     3661 tcacccgctg accgaagcgg atgcctggga aatggtcaaa tcgctcaaga gttacccgct
     3721 cctggccggt ttccgcggcg ccaaaccggt tgatctggag gaactcgtca acgccattct
     3781 gaaactttcc cagctggtgt ccgattttcc gcagatagat caattcgaca tcaacccgtt
     3841 catcgtttca ccccgcaaag atgcaacccg ggcggtcgac gcgcggtttt tgctccgtta
     3901 gactctgtca gcggttgctc tactcatagg ccgatagcca tcattgttcc actggtgagt
     3961 aggggagagc ctcacccggc cggctttttt tctggtgctg gttgtgtaaa ttagtcctga
     4021 tatgccacag cagtctgcag agaccatcat ccaggccgag aaactggcca aaaagtacgg
     4081 cgacctggtg gccgtagacg gcatcgactt cggcatccgc cggggagaga ccttcggctt
     4141 cctaggcccc aatggcgcgg gcaagaccac cacgatgaaa atgatttact gcttcatgcc
     4201 gccgacctgg gggaaacttt cggtagccgg gtacgatgtc acccggaacc cgcgtgaaat
     4261 taaagccctt ttaggcgtgg tgccgcagga ggattcgctc gatcccgatc tcaccgtcta
     4321 cggcaacctc ctggcctatt cacgttacta cgatatatcc ccaaaagtgg cgcacgagag
     4381 aatcgttgaa ctgctcgatt tcatgcagtt gaccgagaag aagaatacca tcatctatgc
     4441 cctctccgga gggatgaaga ggcgtctggt tttagcccgg gggttgatca acagtccgca
     4501 gattctggtg ttggacgagc cgaccacggg gctggatcct caggcgcggc atttagtctg
     4561 gcagaaactg cgccatctga aaagtcaggg gttgacgatc ctcttgacca cccactatat
     4621 ggaagaagcg agtcttctct gcgaccggct ggtcattatg aacgccggta aaattttgga
     4681 cagtggcagc ccgcaggagt tggtcaaaaa agtggtgggg caggaggtgc tggaaattta
     4741 cagcgcggac gacgcgctct tccagaaagt gcgctcaagc gtcggccccc gcgccacggc
     4801 ggagttgttc ggcgacgtca tctacctcta ctccaacgac ggtcagccga ttctctcgga
     4861 gctgccgcgg ttaggcatcg agaacttcct gcaccgccgg gcgacgttag aggatgtctt
     4921 cctcagattg gcggggagga cgttaaaaga gtgattgagg atgggccgat gatgcgtgct
     4981 ttagaaagta cagaaccgcc cttaagggcg gttctgaaaa caactacccc agtgtgctat
     5041 cgggtgaacc taaatgttca cccctacttg atcttcaacc ccgcgaagcg tttttcgatg
     5101 gcgggccagt ccaagttggc gaagaactta tcgatgtagg ccgcacggtt gacgccgtag
     5161 tcgatgaagt aggcgtgctc gtaggtgtcc aaagccacaa ccggcgtggc gttccagatg
     5221 gggaaggtgt tctgcgcgtc gcccaggtaa ttaaagaact tcccgtcgtc gtggtcgtag
     5281 gccagccagg cccagccgcg agccgaaatc ccggtggcct tcagatcttt ctgccaggaa
     5341 tcaaacgagc cgaagtcgcg cttgatcatc gccatcaggt cgccggaggg ctggccgccc
     5401 ttgccgccta agttgtcgaa gtaaatctcg tggttcttca ccccgccgat ggcgaaggtc
     5461 agctcgagtt tcagaacccg caggtcactg tagacctgat tggcggtcga taagtcgacg
     5521 gtcgccagtt tgcctctgat ttcgttggtc ttgttgacgt agcccttgta gagtttctcg
     5581 tgctcctcga tggtcctggc ggagatgccg tccatctgca ggagcgaggg cttgagcggt
     5641 ttgggggtaa tctggtcagg cattgtttga ctcctttctg cgatttgtgc gagcgattcg
     5701 gttgatccga cggtcaaaag cgccgcagcg gaagctgcgc cgccaactaa gacagtgagg
     5761 gcttcgcgcc gggtataggt tgattgcgac tgcgcgtcca tccgtgaaca tcacctcaag
     5821 gcgtaaagga aagcggaaga aatatgacag aatcagtcgg tttgtcaact gacacagggg
     5881 agccgactaa atgttcagtc aggctggaag tcccgccaag agcgagcggg actaaaagcc
     5941 tgacccatgg aagacaaatc gttcctcata gccgagcttc tcggtacgag ccgtattggc
     6001 cagcctcagc acagccggcc tcaaagcaat acttgaatgc caactgattt tcaaagatac
     6061 ttgtatccac tcccggtatg aaagacaacc acgctatcct gctggctaat ccgcgattgc
     6121 tcaaccagtt tcttcaatcc cgccatggtc gcgccggttt ccggacaggc gaagatcccc
     6181 tcggcttgcg ccaacgactt ctgaccggcc tcgatttcct cttcagaaac ggcaaccgca
     6241 gcgccgttgc tttcacggag aaccttgagc atcaagaaat cacccaccgc cgaaggaacg
     6301 cgcaggccga gcgctctggt ctgggggttg ggccaggggg aagtgcgttc ggctttttcc
     6361 tcaaatgcct tcacaatcgg ggcacagccg gaggcttgca cggttaccat tttcggacgt
     6421 tttttaccaa tccagccgag tttttccaat tcgtcgaacg ctttccacat ccccaccaag
     6481 ccggtcccgc cgccggtggg gtagatgacc cagtcgggca attcccagtc gaattgctcg
     6541 gccagttcgt accccagcgt ctttttacct tcgacccggt acggttcttt caaagtggag
     6601 agatcaaacc agccctcttt ttctctctct ttggccattt cctttccggc atcagcgatc
     6661 gatccggaaa cgaggcggac tttggctccg caggcctggc attcctctac gaacgctttg
     6721 ggcgtgtcat ccggtacaaa gacggtgcag tcgatcccgg ctttcgccgc gtaggccgct
     6781 gcggcgcctc cggcattgcc tgcggatgga agtgcgactt tggtgattcc cagttccctg
     6841 gccttcgaaa tcgccacagc catccccctc gctttgaacg agccggtggg attacctgat
     6901 tcatctttga tccagagatt ttcaaggccg aggcgggcac ccagctggtc ggctttgatg
     6961 agcggcgtga atccttcacc gagagtcacg acattacgct cgtcttcaac cggcaggagt
     7021 tcccggtaac gccagagaga ggcggggcgg ctcggcaaat cacttttttt gagttttttc
     7081 gcaatcgaaa caagatcgta ctgagcgtag agcgggccgc cgcaagggca gaggttttgg
     7141 gggcgggaag gatcgtgctc ctggccgcac ttggtgcaga gcagagttct gaggaaggtc
     7201 atgaagtcga gaatatactc cggctgtcat tgcggctcaa tgtttgacac cctgtcgagc
     7261 ggctgttaat tactgttaga ttccaatggc tgagaaaact tcgggtgcca aggcg
//
LOCUS       DBNGZA010000465         3448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1154118, whole genome shotgun sequence.
ACCESSION   DBNGZA010000465 DBNGZA010000000
VERSION     DBNGZA010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1154118"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..2589
                     /locus_tag="AC16FU_09950"
     CDS             226..2589
                     /locus_tag="AC16FU_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR2994730.1"
                     /translation="MLLRQRLLPVLLFAVSVWVVTPAKAGAVDHHITMLAHVVDDTMR
                     GCWGYIDSLGREIALVCSYSNLDLYDVTDPTNPILRKRVPQIADATDLKAVRPYHDSI
                     AYVGNQSGLMQIVYIWNADNAQTIGSFATPLTGGNHTIEIFGDYMYLGMNGASDRRIQ
                     IWSLANPAAPTKIISLANPGTGFNDGHDCYVHGDTIYTHHLTAGFKVWNIANKAAPVG
                     LATVSYPGVFQHASWTSSDGKILYTDDETDFGRLRVWNVSDLRQIIQLANYAPSANAG
                     GVVHNVYLFQGDDLAYTGCYEEGVRILDVSDPTYPIEVGGFDTSPGNNPPGPPSAPQE
                     ARDGVWDAYLFPSGTLIATDRKFGFWTLRFDGSRAGRIEGTVTNSVTGQPLPDAKVKL
                     INLTNLPNVPSVLTGTDGSYGIRTGSVLQNVEVSRFGFRTDTVQVTGIVADTVPLNVA
                     LDPVPYGTISGQVTRSATGDPIAGVIVGLAGEPYHSDTTDGSGNYSINFVPADSNFLI
                     ETWKWGFQSAQATVLVTAGGNATANFGLRSGYSDDFEHDLGWTVGDAGDDATAGFWTR
                     VEPFGMKNFANSTVQPDTDHTAPRGQRCWITGQGRFGIVANDQDVDDGKTTLLSPMMD
                     LSGFSTPVLSYWRWFSNNGDHNPDEDTLEVSISSDGGISWVVLERTTATENRWTSKQF
                     CLTSKINLTSNVRFRVVISDRRGPSLVEGAFDDFAVLDRAAGDINNDRTVDIADITQL
                     ITHVVFGTQPPNPLCFGDLNCDGVIDIVDVVQLILFVVFGAPAPCTP"
     gene            2763..>3448
                     /locus_tag="AC16FU_09955"
     CDS             2763..>3448
                     /locus_tag="AC16FU_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994731.1"
                     /translation="MRKLTLFTLLTLGAPGFFLCSKKPTNPTPPVVVEKPCADLDSTL
                     TFTCCRQWVRDPTWSPDGNTIAYADLEINWGLHLVNPDGSNDHLIVPAVGVFELDFSS
                     DGQWILFLWLRTNQPPGSTGIYKILTSGKDTTKISSTYDDFYPRWSPDDKLIAFERDI
                     SSGDPQNGVYLMNADGTGARQIFPLGQVPDWSPDGRLVVTKGANLYIIDTLGNVLLKL
                     TNFLADSLGRG"
BASE COUNT          735 a         1049 c          926 g          738 t
ORIGIN      
        1 gcctgacagg ccggacaggt gacgcttgaa ttttccatac ccgagaccca aaatagactc
       61 gacaaagaga cttgacaaca gggaatacac tacatatttt atcgctttga gggagggcag
      121 cccgagaggt tgcccttttg tatttgtgtg agcccttgtt gtagatgaga gtgaaggtac
      181 cgccgcacta taaccctaac ccctcaccgg agggaaggag taggtatgtt gcttcgccaa
      241 aggttactgc cggtcctgct gttcgccgtg agcgtgtggg ttgtaacgcc cgcaaaggca
      301 ggtgcggttg atcaccacat cactatgctg gcccacgtgg tggatgacac catgcgcggt
      361 tgctgggggt atatcgactc cttgggtcgg gaaattgctt tggtctgttc ttacagcaac
      421 ctcgatctgt acgacgtaac cgacccgacc aatccaatcc tgcgaaaacg agtaccccag
      481 atcgctgatg ccactgacct caaggccgtg cgcccttacc acgacagcat cgcctacgtc
      541 ggcaaccaga gcgggctgat gcaaatcgtc tacatctgga acgccgacaa tgcgcagacc
      601 atcgggagct ttgccacccc gctcaccggc ggcaaccaca ccatcgagat tttcggggac
      661 tatatgtact tggggatgaa cggggccagc gaccggcgta ttcagatctg gagtctggcc
      721 aatcccgcgg ccccgaccaa aataattagc ctggctaatc ccggaaccgg cttcaacgac
      781 ggtcacgatt gctacgtgca tggcgatacc atctacactc accacctcac cgccggtttc
      841 aaggtgtgga acatcgccaa caaagctgcc ccggtcggtc tggcaaccgt ctcctatcca
      901 ggcgtcttcc aacatgccag ctggacttct tccgacggta aaattctcta caccgatgac
      961 gaaactgatt tcggccggtt gcgggtctgg aatgtgagcg acctgcgcca gatcattcaa
     1021 ctggcgaact acgccccctc cgccaatgcc gggggtgtgg tgcacaacgt ttaccttttc
     1081 cagggagatg acctggccta caccggctgc tacgaggaag gcgtacggat tctcgacgtc
     1141 tccgacccga cctacccgat tgaagtgggc ggtttcgaca cctcaccggg caacaacccc
     1201 ccgggtccgc cttcggcacc gcaggaagcc cgcgacgggg tctgggacgc ctaccttttt
     1261 ccttctggaa cgctcattgc cacggaccgc aaattcggat tctggacttt gcggtttgac
     1321 ggctcccggg cggggcggat tgaaggcaca gtcaccaata gcgtcaccgg tcagccgctg
     1381 ccggacgcga aggtgaaact gatcaactta accaacctgc ccaacgttcc cagtgtgctc
     1441 accggaaccg acggttcgta cggcatccgc accggcagtg tcctgcagaa cgtcgaagtg
     1501 agccgcttcg gattccggac cgacaccgtg caggtgaccg gcatcgtggc cgatacggtg
     1561 ccgctgaacg tggccttgga tcccgttccc tacggcacca tttcgggtca ggtgacccgg
     1621 tcggccaccg gcgacccgat tgcgggcgtc atcgtcggcc tggcgggcga accctaccac
     1681 tccgacacca ccgacggttc gggcaactac tcgatcaact ttgttccggc cgattcgaac
     1741 ttcttgatcg aaacctggaa gtggggcttc cagagcgctc aggccaccgt actggtcacg
     1801 gcgggaggga atgccaccgc caactttggc ttgcgcagcg gttactcaga tgacttcgaa
     1861 cacgacctgg ggtggacggt cggggatgcg ggtgacgacg ctaccgctgg tttctggacc
     1921 cgcgtggagc cctttgggat gaaaaatttc gccaactcca ccgtgcaacc cgataccgat
     1981 cacactgccc cgcgcggcca gcgctgttgg attaccgggc aggggcgctt cggcatcgtg
     2041 gccaacgatc aggacgtgga cgacggcaaa accacccttc tttccccgat gatggacctg
     2101 tccgggtttt ccacaccggt gctttcgtac tggcgttggt tctcgaacaa tggcgaccac
     2161 aatcctgacg aggacaccct cgaggtctct atttcctcgg atggtggtat ctcctgggtt
     2221 gttctcgaac gcaccaccgc taccgagaac cgctggacct ccaagcaatt ctgcctcacc
     2281 tcgaaaatca atctgacgag caacgtccgt ttccgggtag tgatctctga ccgccggggg
     2341 ccctccttgg tggagggtgc ttttgacgac ttcgcggtgc tcgaccgggc tgcgggtgat
     2401 atcaacaacg accgcactgt ggacatcgcc gacataacgc aactcatcac tcacgtggtg
     2461 ttcggtaccc agccacccaa tccactctgc tttggcgacc tcaactgcga cggggtgatc
     2521 gacatcgtcg acgtcgtcca gctgattctc ttcgtcgtct tcggcgctcc tgcaccctgc
     2581 accccgtgag gaaaaaagaa gcagaaaccc ccgccgaaag ttaccgaggc gggggttttc
     2641 ttttgggaat tttcatgagg aaacccctgg ggaaaagccg gcctcaaaaa aacttgacaa
     2701 aacgaccttc tatttcgtat tctttcatcg gaaattactc gcccgccgga gatagacgga
     2761 aaatgagaaa attaaccctc tttaccctcc tcaccctcgg agctcccggc ttcttcctct
     2821 gctccaagaa gcccaccaac ccaaccccgc cggtggtggt ggaaaagccc tgcgccgatc
     2881 tggattccac cctcactttc acctgctgtc ggcagtgggt ccgtgatcct acctggtccc
     2941 ccgatggaaa tacgatcgct tacgcagact tagagatcaa ctggggcctg catcttgtca
     3001 acccggacgg gagtaatgac catcttatcg ttccggccgt tggggttttc gaactggatt
     3061 tttccagtga cgggcagtgg atactcttcc tttggcttcg cacgaatcaa ccaccgggat
     3121 caaccggtat ttataagatt ctaacctcag ggaaagatac aacaaaaatt tcaagcactt
     3181 atgacgattt ctacccccgt tggtcgcccg atgataaatt aatcgcattc gagagggaca
     3241 tatcttcagg cgatcctcag aatggggtct acttgatgaa cgccgacggc accggagcac
     3301 gccagatctt tccgttaggc caggttccag actggagccc tgatggacgc ttggtggtta
     3361 ccaaaggcgc taacttgtac atcatcgata ctcttgggaa tgtcttattg aagcttacta
     3421 acttcctcgc cgactccctg gggcgggg
//
LOCUS       DBNGZA010000466         2991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1154343, whole genome shotgun sequence.
ACCESSION   DBNGZA010000466 DBNGZA010000000
VERSION     DBNGZA010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1154343"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2991
                     /gene="sov"
                     /locus_tag="AC16FU_09960"
                     /gene_synonym="sprA"
     CDS             <1..>2991
                     /gene="sov"
                     /locus_tag="AC16FU_09960"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR2994732.1"
                     /translation="TRQNAYLDDFEGSKETVDLGIFREGWTAASPPVGKGPSSRGRLH
                     WYNPDGQDWEQRFRVKDIWPDRDTRTVDERTDVLKFVFVPKDTGAAPPESSWGGIMRS
                     FFGGGVNLSQAQNLEVWATGDSGILHFDFGKIQEDLDSNGILNTEDSTVNGIRDNILT
                     PGEDIGLDGQPDGNEPPAFGGTIGDPGGDNWHSRPDQINGTEGNRFDPGRRDRPDTED
                     LNGDGALETHNEYFEFTLDLADTTSPFIVPGTLGRFWSQPGNDFFTGTSYGTRWRLYR
                     IPLRDSLIYRKEGRPDWNSIEYVRFWVTGVRDTARFAIATQAVVGNRWRELGLTPKDS
                     ISPDEKFRVAVINSQENKNYQIPPGVTETIDRITNVRQQEQSLVLQYENLGPGHTGLA
                     QRTILGTAEDYSGYRRMHMFVHGPSGPGELLFFLRFGSGTNADTNNYYEYRTKVYPGW
                     DSRNEVVIDFDRMTEIKGLLLKNHPSGGAQVPDTLTGNYRIRGNPTLGSVRWFTMGVT
                     NADTAGGPPASGEIWADELRVTDVRRDAGLAARFSAGAQFSDFIDLKVDYLHRDAFFR
                     NLTSGDRSGSLAASSYRFSSTQNNLTVSTQLALQKFLPPRWGATLPLSINYRNGSSVP
                     RLKNGSDIVLAPALRAEESTTNQDFNLTLSHQFRIETKNWLVNLTLNRLGQFTYAYGR
                     SQSHTPTLPKNDQSRYDVSGNYDLSPRTQLGIPWLAWSKWLLVPRSIAKSKLQLLPSS
                     FTLRGSLSRVLTEQVNSSQRPTTVFTRNFSGGWSTRFQPFSPLSVTLDGTTQRDLANP
                     DNLHFSLNPKTARFGFETSRTQAFGANYTPRIFSFMDNSLSYSANYAENANPQQTVYG
                     RTVTLTSGYGASSLVNLPNLYQATLGHFSFGKPAQQPKKPATVPQSVSPPAGANRDSL
                     ERAGRTLSADSTDSVKAGAPKGPGFSPLRLVSRSLYRGLQIIDPVRLSYKKDRNLSRQ
                     ALPGSPQQTFRFGFSEKTGFSTTS"
BASE COUNT          622 a          980 c          831 g          558 t
ORIGIN      
        1 acaccagaca gaatgcctac ttggacgatt ttgaggggag caaggaaacg gtcgatctgg
       61 gcattttccg tgaagggtgg accgccgcca gcccgccggt cggcaaaggt ccctccagcc
      121 gcggccgttt acactggtac aacccggacg gccaggattg ggaacaacgc ttccgagtca
      181 aagatatctg gcccgaccgg gatacccgca cggtcgatga acgcactgat gtactgaaat
      241 ttgtctttgt accaaaggac accggtgcgg cgccgcctga gtcttcctgg ggtggcatta
      301 tgcgctcctt ctttggcggc ggtgtgaacc tctcgcaggc tcaaaacctg gaagtttggg
      361 ccaccggcga ttcgggcatt ctccatttcg actttggaaa aatccaggag gatttggaca
      421 gcaacggtat actcaacacc gaagacagca cggtcaacgg cattcgggac aacatcctga
      481 ccccgggtga agatatcggg ttggatgggc agccggatgg gaacgaacca ccggcgttcg
      541 gaggaacgat cggtgatccc ggcggggaca actggcatag cagacccgat caaatcaacg
      601 gaactgaagg aaatcgcttt gaccctggcc ggcgcgaccg gcccgatacc gaagatctca
      661 atggtgacgg tgcgttagag acgcacaacg aatactttga gttcacactc gatttagctg
      721 ataccacctc ccctttcata gttccgggga ccctgggacg cttctggtcc caacctggca
      781 acgacttttt tacagggaca tcctacggaa ctcgctggcg gctctaccgc attcccttgc
      841 gggatagtct catctaccgg aaagagggcc gtccggactg gaactcgatc gagtacgtcc
      901 ggttctgggt gaccggagtt cgggatacgg cccgctttgc cattgccacg caggccgtgg
      961 tcggcaaccg ctggcgggaa ctggggctta caccgaaaga cagtatctcc cccgacgaaa
     1021 aattccgggt ggcggtcatc aactcccagg agaacaaaaa ctaccaaatc ccgcccggcg
     1081 tcaccgaaac cattgaccgg atcaccaacg tgcgccagca ggagcagtcg ttggtgctgc
     1141 agtatgaaaa cctcgggccc ggacacaccg gtctggccca gcggacgatc ctgggcaccg
     1201 ccgaagatta ttccggctac cggagaatgc acatgttcgt ccacggtccg tccggccctg
     1261 gagaactcct ctttttcctg cgcttcggat cgggcaccaa cgccgacacg aacaactact
     1321 acgagtaccg gaccaaagtc tatcccggct gggacagccg caacgaagtg gtgattgatt
     1381 ttgaccggat gaccgaaatc aaagggcttc ttttgaaaaa tcacccctcc gggggagcgc
     1441 aggttcccga cacgttgacc ggcaactacc ggatccgggg gaatcctacc ctgggctcgg
     1501 tgcgctggtt caccatggga gtgaccaacg cagacaccgc gggcggcccg cccgcttcag
     1561 gcgagatctg ggccgacgaa ctccgcgtaa ccgacgtccg ccgggatgcc ggcctggcgg
     1621 cccggttttc cgccggcgcc cagttttccg acttcatcga cctcaaggtc gactacctcc
     1681 accgcgacgc gttcttccgc aacctgacct ccggcgatcg gagcggcagt ctggccgcct
     1741 cgtcgtaccg tttctccagt acccagaaca atttaaccgt gtcgacgcag ctggccctgc
     1801 agaagttttt accgccgcgc tggggggcca cccttccctt aagcattaac taccgcaacg
     1861 ggagctcggt gccgcggctg aagaacggtt cggacatcgt gctcgccccg gccctgcggg
     1921 ccgaggaatc gaccaccaac caggacttta acctcacgct ctcccaccaa ttccggatcg
     1981 agacgaaaaa ctggctggtg aacttgacct tgaaccggct ggggcaattt acctatgcct
     2041 acggtcgcag ccagagccat accccgacct tgcccaaaaa cgatcagagc cgctacgacg
     2101 tctccgggaa ctacgacctg tcgccccgca cccagctcgg cattccctgg ctggcctggt
     2161 cgaagtggct tttggttccg aggtccatcg ccaaatccaa actccagctc ctgccgtcat
     2221 cgttcaccct gcgcgggtcg ctctcccggg tcctgaccga gcaggtcaac tccagtcagc
     2281 gtccgaccac ggtctttacc cgcaattttt cagggggctg gtcgacgcgt ttccagccgt
     2341 tttccccgct gagcgttacc ctggacggca ccacccagcg cgacctggcc aatccggaca
     2401 acttgcattt ctccctgaac ccgaagaccg cgcgttttgg ctttgaaacc agccgcaccc
     2461 aggcctttgg tgccaactac acgccgagaa ttttcagttt catggacaat tcgctgtcct
     2521 acagcgccaa ctatgcggaa aacgccaacc cgcagcagac ggtctatgga cgcacggtga
     2581 ccctgacttc cggctacggc gcctcctcgc tggtcaattt gccgaacctc tatcaggcga
     2641 ctctgggaca tttcagtttc ggcaagccgg ctcaacagcc gaaaaaaccc gccaccgttc
     2701 cgcagagcgt cagcccgccg gcgggagcga accgcgacag cctcgagcgg gcgggccgca
     2761 ccctctcggc cgacagcacc gactcggtca aagccggcgc tccgaaaggg ccgggcttct
     2821 ccccgctacg cctcgtctcc cggtcgctgt accgcgggtt gcagatcatt gatccggtcc
     2881 gcttgtccta caagaaggat cgcaacctgt cccgccaggc gctgcccggc agtccgcagc
     2941 agacgttccg gtttggcttc agcgaaaaga ccggcttcag cacaaccagt c
//
LOCUS       DBNGZA010000467         3048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2744620, whole genome shotgun sequence.
ACCESSION   DBNGZA010000467 DBNGZA010000000
VERSION     DBNGZA010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2744620"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="AC16FU_09965"
     CDS             complement(<1..193)
                     /locus_tag="AC16FU_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994733.1"
                     /translation="MLKKFSGNLLYILITLLVLTLYLDHHDSLAKYEVKIQYLMLKFR
                     GKSDPGKDVVVVAVDDQAIN"
     gene            complement(267..995)
                     /locus_tag="AC16FU_09970"
     CDS             complement(267..995)
                     /locus_tag="AC16FU_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR2994734.1"
                     /translation="MKLGFFSDVHGNLEALEAVLADCAAEKLEKLFFLGDAVGYGPSP
                     NECVRLVAEAAEVSLMGNHDYAALGLLELDLFNHYAKQTLDWTRRVLDERAQEILSGF
                     QLDHRVADCYLVHASPHEPLNWDYVLTLEDAEHCFPFFREPACLIGHTHRPALVKKTG
                     AQPCTLLADTGVKIEGGSRYLINIGSVGQPRDGNPKASYLVYDTERHQATLKRVAYDF
                     QKVQQKMAEFQVPTFLIERIATGR"
     gene            complement(992..1687)
                     /locus_tag="AC16FU_09975"
     CDS             complement(992..1687)
                     /locus_tag="AC16FU_09975"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR2994735.1"
                     /translation="MPEILVKYEDKIIERVVTEKKRISIGRTSDNDIVLDNRGVSRKH
                     AQIEFNGNTAVILDNESLNGTFVNNRKVAEEILKDNDVITIGKYQLIYHVAAGHDYKP
                     TDMDGTMVLKTKKQRELLEKDLRDRQIVTKYGGPVLQGVAHCEAEQFKLDRDVVTFGK
                     SSFVNIQVKGFWVSGIQAKLIREDSRFVLVNVGRRGRVKVNGEPVERVGLRNDDLLEI
                     GRSVFRFVEGSSR"
     gene            1917..2681
                     /gene="larB"
                     /locus_tag="AC16FU_09980"
     CDS             1917..2681
                     /gene="larB"
                     /locus_tag="AC16FU_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR2994736.1"
                     /translation="MHPETLARLLRQIRTRKISPPQALERLKLLPFEDLGFATIDHHR
                     HLRQGLPEVILGTGKTAPQVEKIAERLLAAGAPVLATRLDEPVRKILREHFPWARENA
                     LARAVFISSTKLAKKTRGGGRLLIISAGTADQPVAEEASFAGEVIGLRTKRAFDVGVA
                     GLHRFFAHTDSLRWADVIIVVAGMEGALPSVVGGVVGKPVIAVPTSVGYGTSFGGLTA
                     LFAMLNSCAAGVTVVNIDNGFGAAFAAWRILQVKPK"
     gene            2678..>3048
                     /gene="larC"
                     /locus_tag="AC16FU_09985"
     CDS             2678..>3048
                     /gene="larC"
                     /locus_tag="AC16FU_09985"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HIR2994737.1"
                     /translation="MKIAYCDCIGGAAGDMLLGALVASGLKLEALQKEIAKLSLPEVR
                     VTVHPVKRQALAAAQIEISAPEAQAYRHLKEIEQIISTSGLADAVKLKSLAIFHRLAE
                     AEARVHGSSVEEIHFHELGAV"
BASE COUNT          577 a          916 c          913 g          642 t
ORIGIN      
        1 ggttaatggc ctggtcgtcc acggccacca ccacgacatc tttgccggga tccgacttgc
       61 cccggaactt gagcattaag tactggattt tgacctcgta cttggccagt gagtcgtggt
      121 ggtccaggta gagcgtcagc accaggagag tgatgaggat atagaggagg ttgccggaaa
      181 attttttcag catgcgtttg atcctgccgg tttcatttcc ttacgagtac aacaatccca
      241 caggtacggc ccaattcaga gcaggcttac cgtcccgtgg ctatccgctc aatgagaaaa
      301 gttggcacct ggaattcggc catcttctgc tgcaccttct ggaaatcgta cgccacgcgc
      361 ttgagggtgg cctggtgcct ctcggtatcg tagactaaat aactggcttt cggattcccg
      421 tcccgcggct ggcccaccga accgatgttg atcaggtagc ggctgccgcc ctcgattttc
      481 accccggtgt ccgccaaaag cgtacacggc tgggcgccgg tttttttgac cagagccgga
      541 cggtgcgtgt gcccgatgag acaggccggc tcccgaaaaa aagggaagca gtgttcggcg
      601 tcctccagcg tgaggacgta gtcccagttg agcggctcgt gcggggaggc gtgcaccagg
      661 tagcagtcgg ccacccggtg gtcgagctga aaaccggaga ggatttcctg agcccgctcg
      721 tctaagaccc gccgggtcca gtcgagcgtc tgcttggcgt agtggttgaa gaggtcgagt
      781 tccagaagtc ccagggcggc gtagtcgtgg ttgcccatga gcgagacctc cgcagcttcg
      841 gccaccaaac gcacgcactc attcggcgac gggccgtacc ccactgcgtc tcccagaaaa
      901 aaaagtttct ccaatttctc cgccgcgcag tcggccagca ccgcttccag tgcctcgagg
      961 ttgccgtgca cgtcggagaa gaaccccaac ttcatcggct ggagccttct acgaagcgga
     1021 agaccgagcg gccgatctcc agtaaatcgt cattgcgcag ccccacccgc tccaccggtt
     1081 cgccgttcac cttcacccgc ccgcggcggc cgacgttcac cagcacgaat cggctgtcct
     1141 cgcggattaa cttggcctgg atgccggaaa cccagaaccc tttcacctgg atgttgacga
     1201 aagacgactt gccgaaggtc accacgtcgc ggtccagctt gaactgctcg gcttcgcagt
     1261 gggccactcc ctgcaacacc ggcccgccgt acttggtgac tatctgccgg tcgcgcaggt
     1321 ctttttccaa aagctcccgc tgttttttgg tcttcagcac catcgtgccg tccatgtcgg
     1381 tgggcttgta gtcgtgcccg gccgccacgt ggtagatgag ctggtacttg ccgatggtga
     1441 tgacgtcgtt atccttcagg atttcttcgg ccaccttgcg gttgttcaca aaggtgccgt
     1501 tcaagctctc gttgtccagg atcaccgcgg tgttgccgtt gaactcgatc tgggcgtgct
     1561 tgcgcgacac gccgcggttg tccaggacga tgtcgttgtc cgacgtccgc ccgatggaga
     1621 tccgcttctt ctcggtcacc acccgttcga taattttgtc ctcatatttg acgaggattt
     1681 caggcatagg cgctcctttt ccgccgcaga gaatccccca cacttgcccg tctggtcatc
     1741 tctcctatat tgtcggtcgg gtcgagggaa acttaagagt ggccggtcgc tacggcgctg
     1801 tgccgaagaa agttgtcggt tgcgcaatat ctgcgcagca ccggaagacg gtaccccggc
     1861 ccgaaacagt ttccgcaccc gcccgagccg atgttggttt cgtttccgtg gaacgtatgc
     1921 atccagagac tctagcccgg ctcctgcgcc agatccggac gagaaaaata tctccgccgc
     1981 aagctctcga acggttgaaa ctcttaccct ttgaggatct cggctttgcg actattgacc
     2041 accaccgcca cctgcgccag ggactcccgg aagtgattct gggcacaggc aaaaccgccc
     2101 cgcaggtgga gaagatcgcc gaacggctcc tggcggccgg agcgccggtc ctggccacgc
     2161 ggttggatga accggtacgg aagatcctcc gcgagcactt cccctgggca cgggagaacg
     2221 ccttagcgcg ggcagtcttc atatcctcaa cgaaacttgc gaagaaaact cgggggggcg
     2281 gacgtttatt gataatttct gccggtacgg ctgaccagcc ggtggccgaa gaggccagct
     2341 ttgccggcga ggtgatcggc ctgcggacca aacgggcgtt tgacgtcggc gtggccggtc
     2401 tccaccgttt ctttgcgcac actgactcgt tgcggtgggc ggatgtgata attgtcgtgg
     2461 ctgggatgga aggcgccctg ccctcggtcg tcggcggcgt ggtgggtaaa ccggtgattg
     2521 cagtgccgac gtcagtcggg tacggcacca gtttcggcgg cttgaccgcg cttttcgcca
     2581 tgctcaactc ctgcgcggcg ggcgtcacgg tagtgaacat cgacaacggc ttcggcgccg
     2641 ccttcgccgc ctggcgcata ttgcaagtga agccgaaatg aaaatcgctt actgcgactg
     2701 catcggcggc gccgccggcg atatgctcct gggggcactg gtcgccagcg gcctgaaact
     2761 cgaggcactc cagaaggaaa ttgcgaagtt atctcttcca gaggtcaggg tcacggtgca
     2821 tccggtgaaa cggcaggcgc tggcggccgc gcagattgaa atctcagcgc cggaagccca
     2881 agcgtaccgc catttgaagg aaatagagca aattatttcc acgagcggac tggccgacgc
     2941 ggtcaaactg aagagtctgg ccatcttcca ccgcttggcc gaggccgaag cccgcgtgca
     3001 tggaagttcg gtggaggaaa ttcacttcca cgagttaggt gcggtgga
//
LOCUS       DBNGZA010000468         4391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1788737, whole genome shotgun sequence.
ACCESSION   DBNGZA010000468 DBNGZA010000000
VERSION     DBNGZA010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1788737"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..922)
                     /locus_tag="AC16FU_09990"
     CDS             complement(11..922)
                     /locus_tag="AC16FU_09990"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF053157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR2994738.1"
                     /translation="MTRDWEVQFREWAKPLGQTEQDRCDNAVSAIRNAIKASEKLRAR
                     GVLPSSVFAQGSYRNNTNVRQDSDVDIGILCTASFCYVLPEHTTAETFGIIPASYLYG
                     QYKNEVEEALVDYFGRSAVKRGNKAFDISETSYHVEADVAAFFEHRSYHSDGTYLKPE
                     GVALRTDKENRRVINWPEQHYENGVNKNKQIGTRFKSFVRILKALSIEMTEQGVRAGN
                     IPGFLLECLVWNVPNDRFQHNTYTDGVKAVLVFLYESTKTDELCREWVEISGLKRLFR
                     PTQKWTREQANAFTMAGWNYAGLGGQS"
     gene            complement(1107..1562)
                     /locus_tag="AC16FU_09995"
     CDS             complement(1107..1562)
                     /locus_tag="AC16FU_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020409185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR2994739.1"
                     /translation="MALVRWRPARELSLFPEDFNRMFEDFWGRFPRFFGNDFGDGGTW
                     APAVDVTETKDDVIVKAEVPGMTKDDIKVTLQDNILTIQGEKKEEREEKEASFHRVER
                     SYGSFVRSFSLPTTVKSDKIHAAYKDGVLRITLPKVEEVKPKEIAVSVN"
     gene            complement(1829..4057)
                     /locus_tag="AC16FU_10000"
     CDS             complement(1829..4057)
                     /locus_tag="AC16FU_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR2994740.1"
                     /translation="MTSVAIFREEELRTNLRDLAIEVLRELWMSSRKVGLYSFDHPGV
                     QRAAAKPFFYLVRYFKYKSAFSCVIQDRRLFVGGTPVPPLVFTKGLVAEWERAAVDSV
                     LFRSEVTVNDLAVFLDRLSKRFPPSDYHNQYLQYLTKRKVFSIEINGDLAKKIFSRSN
                     LYDPELEYDFSVEGMAAELLRREAALATALLAGEWKSDREALEEGGVEFRLAVLREFA
                     GAVIGTYSEEKLLALVESQISGEDLNLLIEKSERVAALKNLLRSLAQHPQKDKVFGKV
                     KELFTARGVQREIYEQTFDLAALFRFRSSEETEALINKVFSSAFAPDDFERFREVFPR
                     LVASRQEEKLHEIFRVLLLNLTREDHSARQKALFLIRATLESLDTLRDEGNFDFLIDD
                     LLTFVAEKKETFEFSEVFDSCLKICLKNRRYQPVAKMALALRQRLGGQNPDDAFGNVV
                     AQKALEFFGADAVVEQLVGEIVEGKTTQIQAIRQALTAINNQKVALALAEVVTHPDRA
                     VRQNALKILLHLGSNSLETFSRILNDGQTFIRQPDGNTLTQAAWFRVRNAIHVLGNLR
                     DERALEVLGRSAIDPDPRVRGEVVRALEKIGGTKAGIILKQLASDRDGEVRKTAIITL
                     GLVGTPDDVIFLRELFLLGSPERLPIFYAAGNLGGVQAKAFFLELLQPNSAAARLAFG
                     RGDDVQVKAAIVKGLAKVNDPECAEKVRELGDSLTNTQKVFIKNILSSKTPKKILSRL
                     MQ"
     gene            complement(4063..>4391)
                     /locus_tag="AC16FU_10005"
     CDS             complement(4063..>4391)
                     /locus_tag="AC16FU_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR2994741.1"
                     /translation="KIYSSPVVADSLVFFGCNDRKVYALAQRSGAVVWTHTAGSIVNV
                     PVTPVGKYLICGTTDGTLFALQSDDGVLVWEYQLKGGLAGRPVGSGNQLFVTTTPGRV
                     FCFTLR"
BASE COUNT          926 a         1244 c         1216 g         1005 t
ORIGIN      
        1 ttaaagttct tcatgattgt ccaccaagcc ccgcatagtt ccaaccagcc atagtgaagg
       61 cgttcgcttg ctctcgtgtc cacttctgcg tcggacgaaa gagacgcttc agcccgctga
      121 tttccaccca ctcccggcaa agttcgtcgg tcttcgtgct ctcatacagg aacacaagaa
      181 ccgccttcac gccatcggtg taggtgttgt gctgaaagcg gtcattcggc acgttccaga
      241 caaggcactc tagcaggaag ccgggtatgt tccccgcgcg gacgccctgc tcggtcattt
      301 cgatggaaag agctttgaga atccggacga aggacttgaa ccgagtgcca atctgcttgt
      361 tcttgttaac cccgttttcg taatgctgtt ctggccagtt tatgacccgg cggttttcct
      421 tgtccgttcg gagtgcaaca ccttcgggtt taaggtatgt gccatccgag tggtagctgc
      481 gatgctcaaa gaacgccgct acgtcggctt caacatggta gctcgtttcg ctaatgtcga
      541 aagccttgtt gccacgcttg acagcagagc gcccgaaata gtccacaagg gcttcttcga
      601 cttcgttctt gtattgcccg taaaggtaac ttgcgggaat gattccgaag gtctcggcag
      661 tcgtgtgctc gggcaggacg tagcaaaatg aggccgtgca aaggattcct atgtcaacgt
      721 cactatcttg gcggacgttc gtgttgttcc gataggagcc ctgggcaaag accgacgagg
      781 gcaaaacccc acgggcacgg agtttttcgc ttgccttgat tgcattgcgg attgcggaca
      841 cggcattgtc gcaccggtct tgttcggtct gccccagcgg cttcgcccac tctctgaatt
      901 gtacttccca atcgcgcgtc attattatct ttcgtgctgc tgtgagttaa ttgcagcgac
      961 cctcgcggtt ccatgtctgc caataaacag caaacttact ctgaatgcca taaatttcat
     1021 tttcatctac ctccctacaa tctgcccgag gggcgggagt gggtgaattt tttccttcca
     1081 cccacgcacc cgccctcgtg accagattag ttcaccgaaa ccgcgatctc cttcggcttc
     1141 acctcttcca ccttgggcag ggtgatccgc agaacgccgt ctttgtaggc ggcgtggatc
     1201 ttgtcggatt tcaccgtggt gggtagcgag aaggagcgca cgaaggaacc gtacgaccgc
     1261 tccacccggt ggaacgacgc ctctttttct tcgcgctctt ctttcttttc gccctggatg
     1321 gtgaggatgt tgtcctgaag cgtgactttg atgtcatcct tggtcatacc ggggacctcg
     1381 gctttgacga ttacgtcatc cttggtctcg gtgacatcca cggccggagc ccaggtgccg
     1441 ccgtcgccga aatcgtttcc aaagaaacgc gggaagcggc cccagaagtc ttcgaacatc
     1501 cggttgaagt cctccgggaa caaggagagt tcgcgcgctg gacgccatct gactaacgcc
     1561 atatctgtca cctcctttcg atctagcttt ttggttacaa tcctgatggt cactcaatcc
     1621 tcgtctgttt ctacaaggaa gtatgtcaaa tctcgtgcca agtttttcga ttagagtaac
     1681 taattggtaa tcagtgagtt gtcaaataat ttaaggtttt gaattattac gaactgccaa
     1741 tacggtgcca ttttgacagg taaatatgta tgaaaggacc gttgtcactg ctgttctggc
     1801 ggaacttaaa acttgagggt ggacccgact actgcatcaa ccggctgagg atttttttcg
     1861 gcgtttttga agagaggata tttttgatga aaactttctg ggtatttgtc agcgaatcgc
     1921 ccagttcccg gaccttctcg gcgcactccg ggtcgttgac tttggccagt cctttgacaa
     1981 tggcagcctt gacctgcaca tcgtcccccc ggccgaaggc cagacgggcc gccgccgaat
     2041 tgggctgtaa aagttctaaa aagaaggcct tggcctgcac tccgccgagg ttgccagcgg
     2101 catagaagat cgggagccgc tccggcgaac cgagaaggaa gagctctctc aggaaaatca
     2161 cgtcgtcggg ggtaccgacc agtcccaggg tgatgatggc ggttttgcga acttcgccgt
     2221 cccggtcgct ggccaactgc ttcagaatga taccagcttt agtcccgccg attttttcca
     2281 gcgcccgcac cacttcgccc cggacgcgcg gatccggatc aatggccgag cggcccagaa
     2341 cctccagcgc ccgttcatcc ctcagattgc ccagaacgtg gatggcattc cgcacccgaa
     2401 accaggccgc ctgagtcagc gtgttgccgt cgggctgacg gatgaaggtc tgaccgtcgt
     2461 ttaagatccg ggagaaggtc tccagcgagt tactgcccag gtgcaaaagg attttcaggg
     2521 cattctgccg cactgcccgg tcggggtggg tgacaacttc agccagagcc aaagcgacct
     2581 tttggttatt gatagccgtc aacgcctggc gaatcgcctg aatctgggtg gttttcccct
     2641 ccacgatttc tcccaccagt tgctcgacga cggcatcggc gccgaaaaac tcgagcgcct
     2701 tctgggccac gacattgccg aaagcgtcat cggggttctg gccgcccagc cgctgacgca
     2761 gagccagagc cattttggcg accggctgat agcgccggtt tttcagacag attttcaagc
     2821 aggagtcgaa cacctctgaa aactcaaagg tctctttctt ttcggcgaca aaggtcaaaa
     2881 ggtcatctat taagaagtca aaatttcctt cgtcgcggag cgtatctaga gattccaagg
     2941 tggctctgat taaaaagagc gccttctggc gggcggagtg atcttcccgg gttaagttga
     3001 gcaggagcac ccggaaaatt tcatggagtt tttcttcctg gcggctggcc acgaggcggg
     3061 ggaagacttc gcggaagcgt tcaaaatcgt ccggggcaaa ggcgctggag aaaactttgt
     3121 tgatgagcgc ttcggtctcc tcggaggagc ggaagcggaa gagcgcggcc agatcgaatg
     3181 tctgctcgta gatttcccgc tgaacgcccc gcgcggtgaa gagctctttc accttgccga
     3241 aaactttgtc tttctgcggg tgctgcgcca gcgagcgcag gaggttcttg agcgccgcca
     3301 cccgctccga tttctcaatc aggagattta aatcttcacc ggaaatctgg ctttcaacca
     3361 acgccagcag tttttcctcg ctgtaggtgc cgatgactgc gccggcaaat tcgcgcagga
     3421 cggccagccg gaactccacg ccgccctcct ccagcgcctc gcggtccgac ttccactccc
     3481 cggctaagag tgctgtggcc agtgccgctt cgcgcctcag gagttcggcg gccattccct
     3541 ccacggagaa gtcgtactcc agttccggat cgtagaggtt cgagcgggag aatatctttt
     3601 tggcgaggtc accgttaatt tcaatcgaaa agaccttgcg cttggtcagg tactgaaggt
     3661 actgattgtg gtagtcggac ggcgggaagc gcttggagag gcggtccagg aagaccgcca
     3721 ggtcattgac ggtcacctcg ctgcggaaga gcacgctgtc caccgcggcg cgctcccact
     3781 cggcgacgag ccccttggta aaaaccaaag gcggcacggg cgtcccgccg acgaagagcc
     3841 ggcggtcctg gatgacgcag gaaaacgcgg acttgtattt gaagtagcgg acgaggtaga
     3901 aaaacggctt cgccgccgcc cgctgcacgc ccggatgatc gaaggagtag agaccgacct
     3961 tccgggacga catccagagc tcgcgcagga cctcaatggc taagtcgcgc agattcgtgc
     4021 ggagctcttc ttcccgaaaa atggctacgc ttgtcataac ggtcaccgga gggtaaagca
     4081 aaaaacccgg cccggggtcg tggtgacgaa aagctggtta ccgctcccca ctggccggcc
     4141 agccaagccg cctttcaact ggtactccca gaccaggaca ccatcatcgg attggagcgc
     4201 aaaaagagta ccgtccgtgg taccgcagat gaggtatttc cccaccggtg tcaccggaac
     4261 gttcacgatg cttccggcgg tatgggtcca gaccaccgct cctgagcgtt gcgccagagc
     4321 gtaaaccttc cggtcgttgc agccaaagaa aaccagcgaa tcggcaacca caggtgacga
     4381 gtaaattttg c
//
LOCUS       DBNGZA010000469         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1475487, whole genome shotgun sequence.
ACCESSION   DBNGZA010000469 DBNGZA010000000
VERSION     DBNGZA010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1475487"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16FU_10010"
     CDS             complement(<1..611)
                     /locus_tag="AC16FU_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994742.1"
                     /translation="MKKVLGVALLVISFIGLSLPVTAQGQGINFSARFGGMLPTNGEF
                     TSAGSLSDEFDLGLMYGAQVGLNLMPSFAVVAGYDFASLPMSVVPTTGAVPKLSIPHF
                     FVKGHYNFTSLIKNPLNRIKPYLSFGGGLYSWNVTADGGSGNQVFGNGTQLKKTSFGL
                     NFGPGFELSLSNSVALFAEGTYHMVFAKDNRFSGGVTPGNFSVV"
     gene            833..1285
                     /gene="rplM"
                     /locus_tag="AC16FU_10015"
     CDS             833..1285
                     /gene="rplM"
                     /locus_tag="AC16FU_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR2994743.1"
                     /translation="MLRTKKTMKTYVPKVSDVPRQWRLIDAQGQILGRLATRVARLLR
                     GKDKPQFTPHLDLGDPVVVVNARGVRLTGRKESQKTYYWNTGYPQGSRQISFLKYRRE
                     KPEELFRDAVWGMLPHNALGRKLIKKLFVYRDGKHPHQAQKPEPLKLG"
     gene            1301..1693
                     /gene="rpsI"
                     /locus_tag="AC16FU_10020"
     CDS             1301..1693
                     /gene="rpsI"
                     /locus_tag="AC16FU_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR2994744.1"
                     /translation="MAGEVFAAIGRRKTSVASVRLRSGNGQFLVNGQALKEYLGRETS
                     VMVVLQPLEMLGLLGKLDIFCKARGGGLSGQAGAIRLGVARALQQMDPETRKKLKAAG
                     FLTRDPRMVERKKYGRVKARKRYQYSKR"
     gene            1810..>2524
                     /gene="rpsB"
                     /locus_tag="AC16FU_10025"
     CDS             1810..>2524
                     /gene="rpsB"
                     /locus_tag="AC16FU_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR2994745.1"
                     /translation="MTAVDLPALLEAGVHFGHQTRRWNPKMKKFIFTAKNGIYLIDLE
                     KTLLCLDRACAKVREVVTNGGSLLYVGTKRQAAELMKQEALRAGMYYVTERWLGGMLT
                     NFQTIRQSIKRLKDLERMRGDGTFEKLPKKEVLGLEKEITQLEKVLGGVKEMGRLPYL
                     VYVIDTKKEKIAVAEANKLGIPVIAIVDTNSDPDVIDFPIPGNDDSFKAIEMITRQIT
                     DTAAEIRAAREKEKEAARQR"
BASE COUNT          600 a          711 c          772 g          441 t
ORIGIN      
        1 acgactgaga agttcccggg ggtcaccccg ccggaaaagc ggttgtcctt ggcgaagacc
       61 atgtggtagg tcccttcggc aaaaagggca accgagttgg agagcgacag ctcaaacccg
      121 gggccgaagt tcaacccgaa actggtcttc ttcaactgag ttccattccc gaaaacctgg
      181 ttgccggagc cgccgtccgc agtcacattc caggaataga ggccgccgcc gaaggagaga
      241 tacggcttga tccggttgag cggattctta atcaaggaag tgaagttgta gtgcccttta
      301 acgaagaagt gcggaatcga cagcttcggc acggcaccgg tcgtcgggac gaccgacatc
      361 ggcagcgacg caaagtcgta cccggccact accgcgaagg acggcatgag gttcaaaccc
      421 acctgcgctc cgtacatcag tcccaggtcg aactcgtcgg aaagcgatcc ggcggaggtg
      481 aactcgccgt tggtcgggag cattcccccg aagcgggccg agaaattgat cccctggccc
      541 tgcgcggtga ccggcaggct caatccgatg aaactgatca cgagcagcgc gacacccagt
      601 acttttttca ttgacaaccc tcctttccgc tttgcggtta tttcgattat cggtaaggcc
      661 ggcagaaact taaccctgtc gaatgccgcc caaagtgacg gctggttact ctatccatag
      721 cccgaaaaag tacacccgat tcctccagcg tttcccggcc gggcggctct tgacagtttg
      781 agccgcgatt gtatattggg aaatcttttc gagggttatc cccttgaatg caatgttgag
      841 gacgaagaag acgatgaaga cctacgtgcc gaaagtgagc gacgtgcccc gccagtggcg
      901 cttgattgat gcccaggggc agattttagg ccggctggcc acgcgggtgg cccggctgtt
      961 gcgcgggaag gacaagccgc agttcacacc gcatctcgat ctgggcgacc ccgtggtggt
     1021 ggttaacgcc cgcggggtac ggctgaccgg acggaaggaa tcccagaaga cctactactg
     1081 gaacaccggc tacccccagg gttcgcggca gatcagtttc ctcaagtacc ggcgggagaa
     1141 gccggaggaa ctgttccgcg acgcggtctg gggcatgctc ccgcacaacg ctttgggacg
     1201 aaagttaatc aagaagcttt ttgtttaccg ggatggaaaa catcctcacc aggcccagaa
     1261 accggaaccg ctgaagctcg ggtaagcaca aggaggcagt ttggcaggcg aagtctttgc
     1321 agcaattggg cgacggaaaa cttcggtggc aagcgtccgg ctccgatccg gcaacggcca
     1381 gttcttagtc aacgggcaag cgttaaaaga gtatctgggc cgtgaaacct cggtcatggt
     1441 ggtgctccag cccctggaaa tgttggggct cttggggaag ctggacattt tctgcaaagc
     1501 cagaggcggc ggcctctccg gccaggccgg agccatccgg ctcggtgtgg cgcgcgccct
     1561 gcagcaaatg gatcccgaga cgcgaaagaa gttgaaagct gccggctttc tcacccgtga
     1621 cccgcgcatg gtggagcgca agaagtacgg acgggtcaag gcccgcaagc ggtaccagta
     1681 ctcgaaacgt taaagcgagt gggaaggaaa ggaaaaactc acacagactg attccggtgc
     1741 gggtcccccg cctgtggcgg gggtgcagcg gaaggtgacg tccgtggcgg cccaacccga
     1801 aaggacaaaa tgaccgcagt cgatctcccc gcactcctcg aagccggggt ccacttcggg
     1861 caccagaccc gacgctggaa ccccaagatg aaaaagttca tttttaccgc caaaaacggc
     1921 atctacctca tcgatctgga gaaaacgctg ctctgtttgg accgggcctg cgccaaggtg
     1981 cgggaagtgg tcaccaacgg cggcagcctc ctttacgtgg gcaccaaacg ccaggcggcg
     2041 gaactgatga aacaggaagc gctgcgggcc ggcatgtact acgtcaccga gcgctggctg
     2101 ggcgggatgc tcaccaattt ccagacgatc cggcagagca tcaaacgctt gaaggacctg
     2161 gagcggatgc gcggtgacgg cacctttgaa aaactcccca aaaaagaagt cctggggctg
     2221 gaaaaagaaa tcacgcagct cgaaaaagtg ttgggcggcg tgaaggaaat gggccgcctg
     2281 ccctacttag tctacgtgat cgacaccaag aaagaaaaaa tcgcggtggc ggaggcgaac
     2341 aagctgggca tcccggtgat tgcgatcgtg gacaccaact ccgatccgga cgtgattgat
     2401 ttccccattc ccggtaacga cgattcgttc aaagccattg aaatgatcac ccgccagatc
     2461 actgacaccg ctgcggagat ccgcgctgcc cgggagaagg agaaagaagc ggcgcggcaa
     2521 aggg
//
LOCUS       DBNGZA010000470         3116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1576954, whole genome shotgun sequence.
ACCESSION   DBNGZA010000470 DBNGZA010000000
VERSION     DBNGZA010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1576954"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..624
                     /locus_tag="AC16FU_10030"
     CDS             43..624
                     /locus_tag="AC16FU_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR2994746.1"
                     /translation="MKRGWLAGLVIAGVILFFVLLTVAYFWTTYNKLVTRNEAIKNAW
                     AQVDNQLERRSDLIPNLVETVKGYATHERELFEKIADARARLAGARSPEEKMTVSNEL
                     SGYLGRLLVVVENYPNLKANESFNNLMFELSGTENRIAVERKRYNDTVLGYNQSLRRF
                     PTNFVASMFRFSQAPYFQVEEGKKEVPKVTFGS"
     gene            630..1047
                     /gene="rnpB"
                     /locus_tag="AC16FU_10035"
     ncRNA           630..1047
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16FU_10035"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1163..2917
                     /gene="recJ"
                     /locus_tag="AC16FU_10040"
     CDS             1163..2917
                     /gene="recJ"
                     /locus_tag="AC16FU_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR2994747.1"
                     /translation="MRRTLARENRAALQWVVAPDPADSGAPTSALARELGLPPALVQI
                     LYSRGYTSAEAIGRFLLPRFSDLCDPFLLPNMQSAIERIVLALAENEKIMVFGDYDVD
                     GLTASALLFLVFNRLGANVSNYVPNRLSEGYGLSPDGFLEAKRRGVTLVVSVDCGITA
                     VDEVAFAREQGIDCVITDHHEPGRVLPKAVAAVNPKLGGEGALGEELSGVGLAFKVAQ
                     ALYRRLGQDELELEEHLDLVALGTSADIVPLIGENRILTKFGLAQIAKTTKPGLKSLA
                     FVAGLMGQEISTGQVVFVLAPRINAVGRLGDAEKAFKLLTTRDELVAAECARHLDAEN
                     RRRKDIDEKTLEEALELIEQQVDFANDRAIILASPGWHLGVIGIVASRLVERFHRPTV
                     LIAIDGAEGKGSARSIPGFHLHDALKECQELLTRFGGHKYAAGLSLPSENIESFRGKL
                     QRVSAERLSEEDLIPKLYLDAEVDFDQIDAKFVELVEQFAPFGPQNMRPTFVTRKAEL
                     FGQPQVVGNGHLKFKARQSASGGDRVFDCIGFGMGHRASELVAGRTKVDLAYVVEFNE
                     WNGARRMQLRLKDLRLSG"
BASE COUNT          650 a          896 c          949 g          621 t
ORIGIN      
        1 caggcatctt ttgatatgtc tggagggaga aagcggatgc ccatgaaacg cggctggctg
       61 gctggcctgg taatcgccgg tgtcatatta ttttttgttc ttttgaccgt cgcctacttc
      121 tggacgacct acaacaaact ggtcacccga aacgaagcga taaagaacgc ctgggcgcag
      181 gtggacaatc aactggagcg gcgctcggac ctgattccga acttagtgga aacggtcaag
      241 ggatatgcga cgcatgaacg ggaactcttc gagaagattg ccgacgcccg ggcgcggctg
      301 gctggcgccc gcagcccgga agagaagatg accgtttcca atgaactttc cggctactta
      361 ggacggcttt tagtcgtggt ggagaactac ccgaacctga aggccaacga gagttttaac
      421 aatctgatgt tcgaactctc cgggaccgaa aaccggattg ccgtcgagcg caagcgctac
      481 aacgatacgg tgctggggta caaccaatcc ctgcgccgct tccccacgaa tttcgtagca
      541 tctatgtttc ggttcagtca ggcgccctat ttccaggtgg aggaggggaa aaaagaagta
      601 ccaaaagtaa catttggatc ataacacccg cagccggtcg gcagccgctt acgacctcga
      661 gtagaggggg aacccctcga tccgcctctg gcggactcgg ggtctaaaga ggaaagtccg
      721 ggctccggcg agccgcagtg cccgctaacg gcggggcgcc ccgagtgatc gggacgacgg
      781 aaagtgccac agagaagaga cttccccgcc gttggcgggg taaaggtgaa acggcggtgt
      841 aagagaccac cgccccgatg gtaacatcgg gggcacggca aaccccactg ggagcaagac
      901 cagataagcg gaaagcaaag gctccttgcg ctagatccgc gggtaggtcg cttgagcccc
      961 gcagcaatgc agggcccaga gaaatggctg cccgtccccc gcctcggcgg gggatcgaca
     1021 gaacccggct tagggccggt tgtgggtgaa aaagcgtaat gcttggtctt ttgggccggc
     1081 ggcaagtttc gtcttccaca aacgagtttc aaaggctgaa tgaaagttcg tccactccgc
     1141 ttcgagaaaa catagattac cagtgcgccg aacgctcgcc cgcgaaaaca gagctgcgct
     1201 ccagtgggtg gtggcgcccg accccgccga cagcggggct cctacctccg ctttagcccg
     1261 tgaattgggc ttgccgccag cgctggtgca aatcctctac agccggggct acacctcggc
     1321 ggaggcgatt gggagatttc ttttgccccg cttctccgac ctctgcgacc ccttcctttt
     1381 gcccaatatg cagtcggcca tcgagcggat cgttttggcg ctcgccgaaa acgaaaagat
     1441 tatggttttc ggcgactacg acgtggatgg cttgaccgct tcggccttac tctttttggt
     1501 gtttaaccgg ctcggagcca acgtctccaa ctacgtcccc aaccgtcttt ccgaaggcta
     1561 cggcctctcg cccgacggct tcctggaagc gaagcgccgc ggcgtgacct tggtcgtttc
     1621 ggtggactgc ggcatcactg cggtcgacga agtggccttc gccagggagc aggggattga
     1681 ctgcgtgatt accgaccacc acgaacccgg acgggtactg ccgaaggcgg tagcagcggt
     1741 caatcccaaa ctcggtggag aaggggcgct cggcgaggaa ctttcgggag tgggtttggc
     1801 cttcaaagta gctcaggcgc tctaccgccg gctgggacag gatgagttag agttggaaga
     1861 acatctcgat ctggttgccc tgggcacgtc ggcggacatc gtgcccctga tcggggagaa
     1921 ccggattctg acgaaattcg gcctggccca aatcgccaag acgaccaaac ccggcttgaa
     1981 gtctctggct tttgtggccg gcttgatggg ccaggaaatt tccacgggtc aggtggtttt
     2041 tgtcttagct ccgcgcatta atgccgtcgg ccgtctgggc gatgccgaga aggccttcaa
     2101 acttttgacc acccgggatg aactggtggc cgccgaatgt gcccgccacc tcgatgccga
     2161 aaaccgtcgg cgcaaggaca tcgatgagaa gaccctggaa gaggcgctgg aattgatcga
     2221 acagcaggtt gattttgcca acgaccgggc catcatcctg gcttcccccg gctggcattt
     2281 gggggtcatc ggcatcgtgg cctcgcgcct ggtggagcgt tttcaccggc ccacggtctt
     2341 gatcgccatc gacggcgccg aagggaaggg ctcggcccgg agcatccccg gtttccacct
     2401 gcatgacgct ctcaaggagt gtcaggaact tttgacccgc ttcggtggac acaagtacgc
     2461 cgccggttta tccttgccgt cggagaacat tgaatctttc cggggaaaac tgcagcgcgt
     2521 ttccgctgaa cggctttcgg aagaggattt gataccgaaa ctgtacctgg atgccgaagt
     2581 tgactttgac cagatcgatg ccaaattcgt cgaactggtg gagcagtttg cgccgttcgg
     2641 ccctcagaat atgcggccga ccttcgtcac ccgcaaagcc gaactcttcg gccagcccca
     2701 ggtggtgggg aatgggcatc tgaaattcaa agcccggcag tccgcctcgg gcggcgaccg
     2761 cgtcttcgac tgtatcggtt tcggaatggg ccaccgggcc agtgagcttg tcgccggtcg
     2821 aacgaaagtg gacttggcct acgtggtgga attcaacgag tggaacggag cccggcggat
     2881 gcaactgcgg ctgaaagacc tgcgcctgtc cgggtgatgg tctgccgtgt cggattgtct
     2941 gtcgtagggg ttcgctcacc gaatccctgc tgttacccca accgcgtggg gcgaccgccc
     3001 tcggtcgccc ccaataatcg gaaaacgtga acactctcaa acgcttccgc cagaaagatc
     3061 cgctggccct ttccaaaata atttcccatg tcgaaaaccg cctgtgggac tacccg
//
LOCUS       DBNGZA010000471         7555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_198180, whole genome shotgun sequence.
ACCESSION   DBNGZA010000471 DBNGZA010000000
VERSION     DBNGZA010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_198180"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC16FU_10045"
     CDS             <1..818
                     /locus_tag="AC16FU_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994748.1"
                     /translation="IAGFTRFSRDTLSNNLESWVTSVQNNLCLKCHDADGAASASTRT
                     QLGTPQRPFTAQTKDAADVNSLLQSSNNFHHAVLTPGNNPYCTPTSTNGNVVTMNPPW
                     NQTVNTHNRISCFDCHAVNGHGGNFSGLLRRETYYKDPVVNPNFANAEKNFCTICHKL
                     TSYYLGSGGSRLNEHDKSEHRTVADGGRNQYGCRGCHAGIYENDANPACDNGSARGTI
                     HGGNFTWPVCSKSPGSASKRFMVGGYLSGWQEGTVNNATCWGGGCHHTNGQQY"
     gene            818..2434
                     /locus_tag="AC16FU_10050"
     CDS             818..2434
                     /locus_tag="AC16FU_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994749.1"
                     /translation="MTVATKISVLIFVAFLVGRNVSFAYPKMYGNYQFNFQRQLLPVG
                     SNNLYNQILYFNLQDYLMVKNRFLFTAYVVRNEYVSNSKRVDFRPRLDFDLTGTPYKL
                     FFSYLPYALPEAGGVKSVYKQYQGLASLHPGNVPDLRFNWNRTDLHDNQPVRRSDASF
                     RTWSVGSAWTKNFFNAALFYTRQENADRLLQEKTLLANAFNVSGGLNLATPGAGYLSS
                     TYNYSHNEREDRGVKTSTANAHSLTTLYATPLAKNLSLSSNYSGRFSFIRQLLPKQKF
                     QDQTLNTSLSFTPLSRMDLSVLRGDMQSLAPGSRTSQDYWAGTANYAFPVWKAVASKI
                     SYSKTWFVQSPQGQFFSDVYYLSLAAPVYPGLQVRADLSVNFRSGALFVPSRYQTSRF
                     LDIRSQPWEKVQLNYYYQSSVAAPNLTFTQVAAGSHNFALNYFPVPTWSATLNYIERF
                     ASQAVGKQRVFAGQIYHTFREQYTINVNYNLSRFSMPLPGQPRQSDNLSSQLVLFLER
                     RTVLTFSYAASNLTVGTFSSTLGATVNYQF"
     gene            2449..3030
                     /locus_tag="AC16FU_10055"
     CDS             2449..3030
                     /locus_tag="AC16FU_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994750.1"
                     /translation="MRKYLGLVVSGAVLWAGCGGSRSFVHPQTDIGFYKKLGVVTFRN
                     LTGDRFAGQKMTSSFVTELLLSKKFDVVEPGEFERIVADVLKGTAGSEEQELNAEQLK
                     AIGEKAGVQGVIEGEVREFQMTRIGSEDFPLISLAIKLVDAPSGQVIWMSSYTAKGGP
                     KFPIFSFGETRTLGELAQKVCRKMVQQFIAQVF"
     gene            3032..3595
                     /locus_tag="AC16FU_10060"
     CDS             3032..3595
                     /locus_tag="AC16FU_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR2994751.1"
                     /translation="MNRSWAFLFPLFCLVGCSHTPRIYLTPQPNVSGDRTVAVLPFEN
                     LSGEEGAGEKVQKIFLVELLKLRKFSVFEPGEVAQALKELRIRATDKLSQEQASQLKE
                     KLGVDWICLGSVLEFAGQRQGTAEVPVVSLTARILDAASVKIIWAAFESRQGDDAEFL
                     FGVGKINSASELAKKIAEKMVVTLNRR"
     gene            3685..4641
                     /locus_tag="AC16FU_10065"
     CDS             3685..4641
                     /locus_tag="AC16FU_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR2994752.1"
                     /translation="MKRVRRAPGFLVLLVTGPLLAQGPQLLVTIKSLVTGYSSALRFS
                     NPQGIFFDRKFKELYVCDAGNHQVVIFDSTGYPIFRFKHWVEQEGKRILGEPRSMVVN
                     AEGDIFLTDNLADYIDVLDHRGESEDQIDLAKLLHLSTAKPDYLALDGEEHLYVGYRG
                     EKSEVAVLDRGYQVIRQLKNQPDNHQDFESISGLWVDAGGKIYVTDVKAEPCVKVFSN
                     TGVYLFGFGRHEVGWGDFSLPAGVATTTDGTIWIVDTFQQVVKAFNSKGEFIQYIGGY
                     GRHPGDMKYPDAIAGDGDQTIFVLERVGERYQEFVVQTQVRQ"
     gene            5117..6190
                     /locus_tag="AC16FU_10070"
     CDS             5117..6190
                     /locus_tag="AC16FU_10070"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994753.1"
                     /translation="MKRLVAGVAGLCLFGLVVVAWAGDYHYGTTLICSDCHVMHFSQT
                     HGYNNDGGGGIDPPLGTQPEPFLLRDEVNKLCLSCHDGEASTPDVYGNNTLVNTRQAG
                     ALNKTTDGGPYYPANGHTLYSKAIAPGGTWAPDTSEGLNCVNCHHQHGFPGGGFSDPG
                     GTGTYRNLMARPGNAPTNRWITYRVGVNDLTKDVFERATGGVATHYAVSNIDFNEPDQ
                     TKSAYGAWCQGCHTNFHGSSADANMRDAGHAAGTGWLRHPTADANIGALTGGHSSLAV
                     LDSNLYRTQVMSASGNWGTQGSTWPGSPTDLTPSCFSCHKGHGNKNAFGLIYALGNAP
                     LGEEGDGTQAKNLCKQCHVQGAD"
     gene            6837..>7555
                     /locus_tag="AC16FU_10075"
     CDS             6837..>7555
                     /locus_tag="AC16FU_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR2994754.1"
                     /translation="MKAQFLNEYDRPIREHYRILVMSWAGWVFDFYDLVLYTFLLIPI
                     GKELNLTNVELSYVLGASLAATAVGGVIFGVLSDRFGRKRVLQWTILTYSVGTFLSGL
                     ASNLPLLISFRIFTGLGVGGEWATGQTYVGETFPPNVRGRYGAFMQTGAPIGIALASM
                     IGGFLAPAIGWRVCFFLSGLPALLVVVVRRSLSESDLWLARKQQMEESGERSKYRHFS
                     KFLTLFSKPHRKLFLLALILA"
BASE COUNT         1702 a         2113 c         2082 g         1658 t
ORIGIN      
        1 ccattgccgg ttttacccgc ttcagccggg ataccctttc gaataatctc gaatcctggg
       61 tgacgagcgt gcagaacaat ctctgcctga aatgccacga cgccgacggg gcggcttcgg
      121 cttcaacccg cacccagctg ggaactcctc aaagaccctt taccgcgcag accaaagatg
      181 ctgcggacgt caacagcctc ctgcagagtt ccaacaattt ccaccacgcc gtcttgacgc
      241 cggggaataa cccctactgc acaccgactt caaccaacgg caacgtggtg acgatgaatc
      301 cgccctggaa tcaaaccgtc aacacccaca atcggatttc ctgctttgac tgccatgcgg
      361 tcaacggcca cggcggcaac ttctccggcc tgctccggcg ggaaacgtat tacaaagacc
      421 cggtggtcaa tcccaacttt gccaacgccg agaaaaactt ctgcaccatc tgtcataagc
      481 tgacgtcgta ctatctcggt tccggcggca gccgtctgaa cgagcacgat aagagcgagc
      541 accgaaccgt ggccgacggc ggccgcaatc aatacggctg ccggggctgc catgcgggga
      601 tttacgaaaa tgacgccaac cccgcctgcg acaacggctc ggcgcgcggc accattcacg
      661 gcgggaattt cacctggccg gtctgctcca aatctccggg cagcgcgagc aagcggttta
      721 tggtgggcgg gtatctttcc ggctggcagg aggggacggt caacaatgca acctgctggg
      781 gtggcggctg ccaccacacc aacggacagc agtactaatg accgttgcga caaaaatctc
      841 cgtcttgatt ttcgtggcgt tcctggtcgg gaggaacgtc tcttttgcct acccgaaaat
      901 gtacgggaac taccagttca atttccagcg gcaacttttg cccgttggtt ccaataatct
      961 ctacaaccag attctctatt tcaatctgca ggactacctc atggtcaaaa accgtttcct
     1021 ctttaccgcg tacgtcgtgc gcaacgaata cgtgagcaat tccaaaaggg tcgatttccg
     1081 cccccggctc gattttgatc tcaccggcac gccgtacaag ctcttcttca gctacctgcc
     1141 ctacgccctc cccgaggcgg gcggggtcaa atcagtctat aaacagtacc aggggttagc
     1201 cagcctccac ccgggtaacg ttcccgatct ccgtttcaac tggaaccgca ccgacctgca
     1261 cgacaaccag ccggtccgcc gtagcgacgc ctcgtttcgc acctggtcgg tgggctccgc
     1321 ctggacgaaa aacttcttca acgccgccct gttctatacc cgccaggaga acgccgaccg
     1381 cctgctccag gaaaagacgc tcttggccaa tgcattcaac gtcagcggag gactcaatct
     1441 cgccacgccc ggcgccggct acttatcttc aacctacaat tactcccaca acgagcggga
     1501 agatcggggg gtgaaaacct cgaccgccaa cgcgcattcg ctgactaccc tctacgccac
     1561 cccgctggcg aaaaacctct cgctctccag caactattcg ggacgttttt cattcatccg
     1621 ccaactgcta cccaaacaga aatttcagga tcaaacgctc aatacgtcac tctccttcac
     1681 gccgctttcc cgaatggatt tgtccgtgct gcggggcgac atgcagagct tagccccggg
     1741 cagccgcacg agccaggact actgggccgg aacggccaac tacgctttcc cggtgtggaa
     1801 agccgtcgca agcaagatct cctattccaa gacctggttc gtccagtcgc cgcaggggca
     1861 atttttttcg gacgtctact acctttcgct cgcggcgccc gtctatcccg gcctgcaggt
     1921 ccgagccgat ctctcggtca atttccgctc cggagcctta tttgtgccga gcaggtacca
     1981 gacgtcacgt ttcctggaca tccgcagcca gccctgggaa aaggtgcagc tgaactacta
     2041 ctaccagtcc agcgtcgcgg caccgaacct cacctttacc caggtggcgg ctggcagcca
     2101 caacttcgcc ctcaactact tccccgtccc gacctggagc gccacgctca attacatcga
     2161 gcgattcgcc tcccaggcgg tgggaaaaca gcgggttttc gccggtcaaa tctaccatac
     2221 cttccgtgag cagtacacca tcaacgtcaa ctacaatctg agccgttttt ccatgccgtt
     2281 accgggccag ccccggcaga gtgataacct gtcgtcccag ctggtcttgt ttctagagcg
     2341 gaggacagtt ttgaccttta gctacgcagc gagtaatttg acggtgggga ccttctcatc
     2401 cacgttgggc gccaccgtga attaccaatt ctgagggcag gggagcagat gagaaaatac
     2461 ctgggtctgg ttgtgagcgg ggcggttctg tgggctggct gcggcggctc gcgcagtttc
     2521 gtgcatccgc agacagatat cggtttctat aagaagctgg gtgtggtcac gtttcggaac
     2581 ctcaccggcg accgctttgc cggccagaaa atgacctcct cctttgtcac cgagctgctc
     2641 ctctccaaga aatttgacgt ggtggagccg ggggaattcg aaagaatcgt cgcggatgtc
     2701 ctgaaaggca ccgcaggatc cgaagagcaa gagctcaacg ccgagcagct gaaagcgatc
     2761 ggggaaaaag cgggcgtgca gggcgtcatc gaaggggagg tcagagaatt ccagatgacg
     2821 cgcatcgggt cagaagattt ccccttaata tcgctcgcca tcaaactggt ggatgcccct
     2881 tccggccagg tgatctggat gtcttcgtac accgccaagg gcgggccgaa attccccatt
     2941 ttctctttcg gcgaaacccg cactttgggg gaactggcgc agaaagtgtg tcggaaaatg
     3001 gtccagcagt tcatcgcgca ggtcttttaa gatgaaccgg agctgggcct tccttttccc
     3061 gctcttctgc ctggtcggct gcagccacac cccccggatt tatctcacac cccagccgaa
     3121 cgtttccggc gaccgcacag tggccgtcct gccgtttgaa aatctctccg gcgaagaggg
     3181 ggccggggaa aaagtccaaa aaatatttct ggtagaactt ctcaagctga gaaaattttc
     3241 agtcttcgaa ccgggggaag tggcccaggc gttgaaagaa ctgcgcatcc gggccacaga
     3301 taagctttct caggagcagg cctcccagtt gaaagaaaaa ttgggggtgg actggatctg
     3361 cctgggttcg gttttggagt ttgccggcca gcgccagggc accgcagaag ttccggtagt
     3421 ttcgctcacg gcgcggatcc tggacgccgc cagcgtcaag attatctggg ccgcttttga
     3481 atcccggcag ggcgatgatg cggaattcct cttcggcgtg gggaaaatca actccgcttc
     3541 agaactggcc aaaaaaatag ccgaaaaaat ggtggtcacc ctcaaccggc ggtagcagcg
     3601 gcagtaagca gctcaccgga cttccccgta tactcaaaga aggcaacctc gcgggcagac
     3661 cgggcgaggt gtgaaatagt ccgtatgaaa agagttcggc gcgctccggg tttccttgtc
     3721 ctcctggtga caggtcccct tctggcacag ggtccccagc ttttggttac catcaaatcg
     3781 ctcgtcacgg gctacagttc cgccctccgt ttttccaacc cccaggggat tttttttgac
     3841 agaaaattca aagagctcta cgtctgtgac gccggcaatc accaggtggt catttttgat
     3901 tccaccggct atcccatttt ccgcttcaag cactgggtcg agcaagaagg gaagagaatc
     3961 ctgggcgagc ccagaagtat ggtggtcaat gcggaggggg acatcttctt gaccgataac
     4021 ttagccgact acattgacgt tttagaccac cggggcgaaa gcgaagacca gatcgacctg
     4081 gcgaagctat tgcatctctc cacggccaag cccgattacc tcgccctgga tggcgaggag
     4141 catctctacg tcggttaccg cggcgagaaa agcgaagtgg ccgttctgga tcgcgggtat
     4201 caggtgatcc gacagttaaa gaatcaaccg gacaaccacc aggactttga gagtatttcc
     4261 ggtctctggg tcgacgccgg agggaaaatc tacgtcaccg acgtgaaggc ggaaccctgc
     4321 gtgaaagtgt tctccaacac cggcgtgtac cttttcggtt ttggccggca tgaggtcggt
     4381 tggggagact tttctctccc ggcaggagtg gccacaacca cagacgggac gatctggatt
     4441 gtggacacgt tccagcaggt ggtcaaagca tttaattcca aaggagaatt catccagtac
     4501 atcggcggct atggccggca tccgggagat atgaaatatc cggacgcaat cgccggagat
     4561 ggcgatcaaa ctattttcgt cctggagcgc gtaggagaaa gataccagga atttgtggtc
     4621 caaactcagg tccgccagtg acgtttagtc cgggctcttt gtctgaggtt ttcgttcgaa
     4681 aagggaaaaa gactgattct atcctcaatc gactcccaag attgcctctt tacctctcga
     4741 gtgagcgttg ctcgaatttt tggcctgaaa atataaggag ttgttttctc gcgtagttac
     4801 ttcaccccca acgacttaga tacaagataa aaagatgtaa atttctcttg acaaattccg
     4861 gagaggtgat ataatagtgc ttgtgagaat gttcacaata cgacgctctg agttctctat
     4921 taagcgacca acggcgaaat gagggatgac gtaggtcata cgagagtgtg tgaaagtatt
     4981 cacaatgttc ggcaactgca ggggaggtga ggagcgacac tcaagagctc tcggcagact
     5041 gggttgtgcg agtggaacga atgagagtga agaattctca gcgaaacggt aacagagcga
     5101 aggaggtgaa aaagaaatga aaaggttagt agcaggagtc gcgggtctgt gcttgttcgg
     5161 actagtggtt gtagcgtggg ccggggatta ccattacggc acaaccttga tctgctcgga
     5221 ctgccacgtc atgcacttct ctcagaccca tgggtacaac aatgacggcg gaggtggtat
     5281 tgatcccccg ctgggaaccc aacctgagcc attcctcctg agagatgagg tcaacaaact
     5341 ctgcctgagc tgccatgacg gggaggcttc tacccccgac gtgtacggga acaacacgct
     5401 ggtcaacacg cgccaggcgg gagcgttgaa caaaaccacg gatggcgggc cgtactatcc
     5461 ggcaaacgga cacacgctct actccaaagc catcgcaccc ggtggcacct gggcgccgga
     5521 taccagcgag ggcttaaact gtgtgaactg ccaccaccag cacggattcc cgggaggcgg
     5581 gttttcggat cccgggggaa caggaaccta ccggaatctc atggcgcggc cagggaatgc
     5641 ccccaccaac cggtggatca cctatcgggt cggagtgaac gacctgacca aagacgtatt
     5701 tgagagagct accggtgggg tcgccacgca ttacgctgtt tccaacatcg acttcaatga
     5761 gccggaccag acgaagtcgg cgtacggtgc ttggtgccag ggttgccaca ccaatttcca
     5821 cggttcttcg gctgacgcca atatgcggga tgccggacac gccgcgggaa ccggctggtt
     5881 gcggcacccg accgcagacg ccaacatcgg tgccctcacc ggcgggcact ccagcttggc
     5941 ggtcttggac agcaacctct accgcacgca agtgatgagc gccagcggca actggggaac
     6001 tcaggggtct acgtggcctg gttcacccac tgacctgacc ccttcctgct tctcctgcca
     6061 caaggggcac ggcaataaga atgcgttcgg actgatttac gctttgggga acgcccccct
     6121 gggtgaagaa ggagatggga cgcaggccaa gaacctgtgc aagcagtgcc acgtgcaggg
     6181 cgcggactga agtcgacgca ggttggttta cccttagagg cgctcgcaaa cgagcgcctt
     6241 taagttttgg aagtggaaat ttctgatctt gtctgggtaa attacttcag ccaatttttc
     6301 ccgggtttct ttctgctcct tgcacctgac atgcgggtgg aaaggggaca ggaggggaag
     6361 ctctattggg cagccggggg gcgcttgaag tcggacgctt cagaagataa gttatcgttc
     6421 caagaacggt tgccggagag gcggaagggt agacgcaggg gacttaaaaa gcctttgcac
     6481 ccttattgca actcgttgga aatcagcaca atattttgag cgccaagtgg atagtggaag
     6541 cgcatgcgag gattatcggg gattgtattg gactattttg ggattattca tagtcacaaa
     6601 ttggtcacaa gagaacgttt catttcctct gacgttttta ctcccctcgc ccaaaaaaca
     6661 ctggttcgaa tcctgccttg ggagaaaagg tatttatttt gagagtcacg aggtaacaca
     6721 gttttgagtc aggaaagtgt ccttcggagg gccggggggc gccttcggga ttttttcttg
     6781 acggttgtgg gtcatcagac ttacttgctt accgaacggc caggccgttt cctgctatga
     6841 aagcgcaatt tctaaacgaa tacgacaggc ccattcgtga acactatcga atattggtga
     6901 tgagttgggc tgggtgggtt tttgattttt acgatttggt actctacacg ttcctcctaa
     6961 ttccgatcgg caaggagcta aatctcacca atgttgagct gtcttacgtt ctgggggcgt
     7021 cgctggcggc tacagccgta ggcggagtca tctttggcgt attatccgac cgctttggcc
     7081 gcaagagggt tttgcagtgg acgattttga cctatagcgt tggtaccttt ctttccggct
     7141 tggcctccaa cctcccactc ttaatctcgt ttcgaatttt caccggcctc ggcgttgggg
     7201 gagaatgggc caccggacaa acctacgtgg gagaaacttt tccacccaac gtccgggggc
     7261 gatatggtgc ttttatgcag accggcgcgc cgattggcat cgccctcgct tcgatgatag
     7321 gaggattctt ggccccggcc atcggttggc gggtctgctt ttttctctct ggactgccgg
     7381 cgctattggt agttgtggtt cgccggtccc tgtctgagtc agacctttgg ctggcaagaa
     7441 aacagcagat ggaagaatca ggtgaacggt caaaatacag acacttttca aaattcttga
     7501 cgctcttttc aaaaccgcac cgtaagcttt tccttttggc ccttatattg gcgat
//
LOCUS       DBNGZA010000472         3753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_198308, whole genome shotgun sequence.
ACCESSION   DBNGZA010000472 DBNGZA010000000
VERSION     DBNGZA010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_198308"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16FU_10080"
     CDS             complement(<1..334)
                     /locus_tag="AC16FU_10080"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR2994755.1"
                     /translation="MPARDRGSSAYLLEVDGRGFLFDTGEGVSQALLRFGKNPNAVER
                     IFLSHFHPDHVMGLPLFIQMVYLLKRTAPLAIYYPEEGKAGLEALFDLTYLLRNRIPF
                     PLEFHTIGA"
     gene            complement(434..874)
                     /locus_tag="AC16FU_10085"
     CDS             complement(434..874)
                     /locus_tag="AC16FU_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HIR2994756.1"
                     /translation="MAKKKIIIAQKDLDIAFADSDPDVRYFLDTISGQIRKINERRTA
                     TGDEEEFSRKELERTGHYIPLPHFSREERKHLIENFAGLQADEAVKDALTACLKQPSP
                     SLALKTLFFDQPELRKGWLLFQGETLRKKIAAILEKEGLELQFV"
     gene            complement(1044..1691)
                     /locus_tag="AC16FU_10090"
     CDS             complement(1044..1691)
                     /locus_tag="AC16FU_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR2994757.1"
                     /translation="MRKAVLSTIAATLLLTGLAWADPTTFYLTSKDRRNLVQFESKAP
                     LETIVGTTGQIEGKLAVDPDNSLSATGSFEVDLTTLKTGIDMRDEHMREKYLETAQYP
                     KATFTLTKITKTAQKKLADLQPVEVTAEGLFSIHGVQKPVTVTGQVTYLKESEQTKVK
                     LPGNLLHIDFTFPLLLTDFGIKVPQMVLLKLDNKQVVKVDAFATTTSPEEMSKRQ"
     gene            complement(1870..2313)
                     /locus_tag="AC16FU_10095"
     CDS             complement(1870..2313)
                     /locus_tag="AC16FU_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994758.1"
                     /translation="MPAFTEKDIKKIATIIGADETTWEKDHFRLQVTNRPERRRLVLE
                     IFPDAQLGAPRKGAMVVVYTGTAHLQLHNCTGYVASEELGEVTFVGEDSGRLSGLVVE
                     QRAACSLYANVNRKVISSDFTKLGVEVMLSGVALSLAEEVLKSTK"
     gene            complement(2314..2511)
                     /locus_tag="AC16FU_10100"
     CDS             complement(2314..2511)
                     /locus_tag="AC16FU_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR2994759.1"
                     /translation="MFGNIGAQELLLILLIVLLLFGARRIPEIAQGLGKGIREFKKGM
                     RDVQEEMNKPESEPKKPEEKK"
     gene            complement(2528..2794)
                     /locus_tag="AC16FU_10105"
     CDS             complement(2528..2794)
                     /locus_tag="AC16FU_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR2994760.1"
                     /translation="MKEREITFLVVALILATIFGGLIGEIVGSFLPEGAAKTLFQKSI
                     EVGFDPQTVKLYAVAFTIGLLIKINFVSVLMVVGVIAYFRWWYL"
     gene            complement(2791..3495)
                     /gene="purQ"
                     /locus_tag="AC16FU_10110"
     CDS             complement(2791..3495)
                     /gene="purQ"
                     /locus_tag="AC16FU_10110"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR2994761.1"
                     /translation="MKFGVVTFPGSNCDYDAFAVLRDVLQQPVEFLWHQEQSLKGCDC
                     IILPGGFSYGDYLRTGAIARFSPIMEKVVSFANEGGLVLGICNGFQVLTEVGLLPGAL
                     LRNAWLRFVCRDVHLRVERTDLPFTCACRPGQVLKVPTAHGDGNYFASQEVLGQLEKQ
                     KQIVLRYCEPDGRITPEANPNGSLANIAGIANERSNVFGLMPHPERAAEALLGSADGA
                     WIFRSLVQHLVQRRAA"
     gene            complement(3492..>3753)
                     /gene="purS"
                     /locus_tag="AC16FU_10115"
     CDS             complement(3492..>3753)
                     /gene="purS"
                     /locus_tag="AC16FU_10115"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR2994762.1"
                     /translation="SNGKKHFKAVVYVSFKDGVLDPQGSTIKRALGTLGYNGIADVRA
                     GKFFEIALLAKDERAAQTEIEEICRKLLANPVIEKYRYEVRS"
BASE COUNT          799 a         1081 c         1055 g          818 t
ORIGIN      
        1 ttgctccgat agtgtgaaac tctagtggaa aaggaatgcg gttacgcaga aggtaggtga
       61 ggtcgaagag tgcttccagt cctgcttttc cctcttccgg gtagtaaatg gccaaaggcg
      121 ccgtgcgctt gagcaggtag accatctgga tgaagagcgg caggcccatc acgtggtcag
      181 ggtggaagtg agaaaggaag attcgttcca ccgcgtttgg atttttgccg aaacgcaaga
      241 gcgcttgtga aacgccctcg ccggtatcga agaggaaacc ccgcccatcc acctccaaca
      301 aataggccga actcccccgg tctctggcgg gcatccccga ggcgcacccg aggacagtcc
      361 aggttatcat ggggagttaa tcgtagccga ggtttttaac ctcggtgccc ccggcgtcga
      421 tcttgattct ctttcaaaca aactgtaatt ccagcccttc cttctccaaa atcgctgcta
      481 ttttcttccg cagcgtctct ccctgaaaca aaagccaccc tttgcgcaat tccggctggt
      541 cgaagaagag cgttttgagc gccagggaag gactcggctg ctttaaacag gcggtgaggg
      601 cgtccttgac ggcttcgtcc gcctgcaaac cggcgaaatt ttcaattaag tgcttccgct
      661 cttcgcggga gaaatgtggc aacgggatgt agtgccccgt gcgttctaat tccttccgcg
      721 aaaactcctc ctcatcgcct gtcgccgtcc gccgctcgtt gattttacgt atttggccgg
      781 aaatggtgtc gaggaagtag cgaacgtccg ggtcagaatc ggcaaaagcg atatcaaggt
      841 ctttctgagc aatgatgatt ttcttttttg ccaccatgtg agtaaaaata gttagaatat
      901 tccgcaaggg aaagaccgga gagaattgta gcgacgctct ccagagcgtc gcgggttcac
      961 gccggtcggg gacccggcca aaaaaatggc gcaggatacg aagatcatcc tgcgccacgg
     1021 agaatgaaat ttacccgcgg ctgctactgc cgcttgctca tctcctccgg cgaggtggtg
     1081 gtggcaaagg cgtccacctt caccacctgc ttgttgtcca acttcaagag caccatctgc
     1141 ggcaccttga tgccgaaatc ggtgagcaag agcgggaaag tgaagtcgat gtgcaagagg
     1201 ttgccgggca atttcacttt ggtctgctcg ctctccttca ggtaggtcac ctggccggtg
     1261 accgtgaccg gcttctggac gccgtggatg gaaaatagcc cttcggccgt cacctccacc
     1321 ggctgcaggt cggccaattt cttctgggcc gtcttggtaa ttttggtcag ggtgaaggtt
     1381 gctttcgggt actgcgccgt ctcgaggtac ttctcgcgca tgtgttcatc gcgcatatcg
     1441 atgccggttt tgagcgtcgt caggtccact tcgaacgagc cggtagcgga cagcgagtta
     1501 tccggatcaa ccgctagctt tccctcaatc tggccggtgg tgccgacgat ggtttccaga
     1561 ggcgccttcg actcgaactg caccaggttg cggcggtctt tgctggtcag gtagaaagtg
     1621 gtcggatcgg cccaagccaa tccggtcaaa aggagagttg cggcgatggt ggagagaacg
     1681 gcttttctca caggtactcc tcgggttacg ggtttgtctt gctttttcgt tgctacgttt
     1741 cgacttgcaa aatcccaaac aaaattgtag cgccactctc ttcaacctga gtccgcctca
     1801 ggcggatcga aggccagagc ggcgggattt gcgccggtca ggagaccggc gctacgcatt
     1861 tattggctgc tactttgttg atttcagaac ttcctcggcc aaagacaaag ccacgccgga
     1921 gagcatcacc tcgacgccca atttggtgaa gtcggaggat atcaccttcc ggttgacgtt
     1981 tgcgtagagc gagcaggcgg cccgctgctc caccaccagc ccggagagcc ggccgctgtc
     2041 ctcgcccaca aaggtcacct cgcccaactc ttcgctcgcg acgtagccgg tgcagttgtg
     2101 cagctgcagg tgggcggtgc cggtgtagac gacgaccatg gcgcctttgc gcggcgcgcc
     2161 caattgggcg tccgggaaaa tttctaagac cagccgccgg cgctccggcc ggttggtcac
     2221 ctgcagccgg aagtggtctt tctcccaggt ggtctcgtcg gccccgatga tcgtggcaat
     2281 ttttttgatg tctttttctg taaaagccgg catctacttc ttttcttcgg gtttcttggg
     2341 ctcgctctcg ggcttattca tctcctcctg gacgtcccgc atccctttct taaactcccg
     2401 gatccctttg cccaaaccct gggcaatctc cggaatgcgc cgggcgccga acagaagcag
     2461 aacgatcagg agaatgagca agagttcctg agccccgata ttgccgaaca agggcatcac
     2521 acctcctcta caggtaccac cagcgaaaat aggcgatcac cccgaccacc atcaagaccg
     2581 acacgaaatt gatcttgatc aaaagcccga tggtgaaagc gaccgcgtac agtttcaccg
     2641 tctggggatc aaaccccact tcaatgctct tctggaaaag cgtcttggcc gccccttcgg
     2701 gcagaaacga accgacgatt tcgccgatca aaccgccgaa gatcgtggcc agaatcaaag
     2761 ccaccaccaa gaaagtaatt tcccgttctt tcacgctgcc cgcctctgta ccagatgctg
     2821 tacgagcgac cggaaaatcc aagcgccgtc ggcggagccc aagagtgctt cggcagcccg
     2881 ctccgggtgg ggcatgaggc cgaagacgtt gctccgttca ttcgcaatgc cggcgatgtt
     2941 ggcaagcgac ccattcggat tcgcttctgg ggtgatgcgg ccgtccggct cacaatagcg
     3001 caagacaatt tgcttctgct tttcaagttg gccgagaact tcctgcgacg caaagtagtt
     3061 tccgtcgccg tgggcagtgg ggaccttcaa cacctgaccc ggccggcagg cgcaggtaaa
     3121 ggggaggtcg gttcgctcga ctcggagatg aacatcccgg cagacgaagc ggagccaggc
     3181 attgcgcaac agcgcgccgg ggagaagccc aacctcggtc aaaacctgaa aaccgttgca
     3241 aatgcccaaa acgaggccac cctcattggc aaaggaaacg actttttcca tgatgggcga
     3301 aaagcgggcg atggcccccg tgcgcaggta gtcgccgtag gaaaagccgc ccggcaggat
     3361 gatacagtcg cagcccttga gggactgctc ctggtgccag agaaactcca ccggctgctg
     3421 cagaacatcc cggagtacgg cgaaagcgtc gtagtcgcag ttggagccgg ggaaagtgac
     3481 gacgccgaac ttcacgaccg gacctcgtag cggtattttt cgatcaccgg attggccaga
     3541 agtttccggc aaatttcttc gatttccgtc tgggccgctc gctcgtcttt ggccaaaagt
     3601 gcgatttcaa aaaacttccc cgcccgcacg tcggcaatgc cgttgtaacc caaggtcccc
     3661 agcgcccgtt tgatggtcga gccctgggga tcgagcacgc cgtccttaaa actcacgtag
     3721 accaccgctt tgaaatgttt tttgccattg gac
//
LOCUS       DBNGZA010000473         1768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_729962, whole genome shotgun sequence.
ACCESSION   DBNGZA010000473 DBNGZA010000000
VERSION     DBNGZA010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_729962"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16FU_10120"
     CDS             complement(<1..255)
                     /locus_tag="AC16FU_10120"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR2994763.1"
                     /translation="MAEPANPKEYRHTFRIAAKPERVFTALTDPKELEKWFAEHVSIE
                     PKPGGRFDFWGKHTLWGLPESSAAQKITAYDPPRRLGFTWE"
     gene            455..1711
                     /locus_tag="AC16FU_10125"
     CDS             455..1711
                     /locus_tag="AC16FU_10125"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR2994764.1"
                     /translation="MTLSEKILSEHAGYAVRAGDLCLVEADFIFAHDLSGPLCISQIR
                     ELGIPKLKYPERVNFFLDHSSPPSTHIAATDHKLIRDFCHERNLKLYDIGSGIAHQVI
                     MEKIARPGDVIIGGDSHTCHVGVFGAFATGMGSTDIAIAMLLGRTWLKVPETLKFEIT
                     GKLRVGVYAKDIMLSIIGKISVSGATYKAMEFVGDTIDSMPLYERAIFPNMAIEAGAK
                     AGLCPSDDMTRQYLREFGREKDYKPIAPDPDAKYKQVFEINVTNLEPTVALPHSMDNV
                     APVTSDKCKDVPVHQVYLGTCTNGRFEDFREAARILKGKRVKAGTRLVCTPASRDVYM
                     KGLKEGLWEIFLESGGLVNSPGCGACPGVHTGILGDGENCISTLNRNFKGRMGNPKAF
                     IYLASPATCAASAIEGKIADPRRYLS"
BASE COUNT          365 a          528 c          480 g          395 t
ORIGIN      
        1 ctcccaggtg aagcccaggc ggcggggtgg gtcataagcc gtaatctttt gtgcggcact
       61 cgactcgggc agcccccaaa gggtgtgttt accccagaag tcaaagcgcc caccgggttt
      121 tggctcaatg ctgacatgct ccgcaaacca cttttccagt tctttcgggt cggtgagcgc
      181 cgtgaagacc cgctctggtt tggccgctat ccggaaagtg tgccggtatt cttttggatt
      241 cgctggttca gccatagtcg attctttctt cgttcagaaa tagagaggat cgatttttct
      301 ctgtgtcctc tgtgtctccg tggtggcttc tattctgttt gtaggcgaaa ttatcgtatt
      361 ccccgagaca gtcaaccggc gcatgcaccg gtcattgccc gtccgttttt cgctccctat
      421 attggttctc gcaacgcacg aggcgcctat gggaatgacc ttatccgaaa aaatcctctc
      481 cgaacacgcc ggctacgccg tccgggcggg cgacctctgc ttggtcgaag ccgacttcat
      541 cttcgctcac gatctctccg gcccgctctg catttcccag atccgcgaat tggggattcc
      601 caaactcaag tatccggaac gggtcaattt ctttttagac cactcctcgc cgccctcgac
      661 gcatatcgcc gccaccgacc acaagttaat ccgcgacttc tgccacgagc gcaacctgaa
      721 actttacgac atcggctccg gcattgcgca tcaggtgatt atggagaaaa tcgcccgacc
      781 gggggacgtg atcatcggcg gcgattcgca cacctgccac gtgggggtct tcggcgcttt
      841 cgccaccggt atgggttcaa cggacatcgc cattgccatg ctcctgggcc gcacctggct
      901 caaggtgccg gagacgctca agtttgaaat caccggcaaa ctccgggtgg gggtctatgc
      961 caaagacatc atgctctcca ttatcgggaa gatttccgtt tccggtgcga cttacaaagc
     1021 gatggagttc gtgggcgaca cgattgactc aatgcccctc tacgagcgcg ccatcttccc
     1081 gaacatggcg atagaagccg gggccaaggc cgggctctgt ccttcggacg acatgacccg
     1141 ccaatacctg cgcgaattcg ggcgggagaa agattacaag ccgattgcgc cagatcccga
     1201 cgccaaatac aagcaagttt ttgaaatcaa cgtaaccaac ctcgaaccga ctgtcgctct
     1261 gccccattcg atggacaacg tcgcgccggt gacaagtgat aagtgtaaag atgtcccggt
     1321 gcatcaggtt tacctcggca cctgcaccaa cggccggttc gaagatttcc gcgaagcagc
     1381 gcgcatcctc aaaggcaaaa gagtcaaagc cggcacgcgg ttggtctgca ccccggcttc
     1441 gcgggatgtc tatatgaaag ggctgaagga agggttgtgg gagattttcc tcgaatctgg
     1501 cgggttggtc aattcccccg ggtgcggggc ctgtcccggc gtccacaccg gcattttggg
     1561 cgacggcgaa aactgcattt ccacgcttaa ccggaatttc aaaggccgga tggggaaccc
     1621 caaggccttc atctacttag ccagcccggc cacctgcgcc gcctcggcaa tcgaaggaaa
     1681 aatcgccgac ccccggaggt atctgtcatg accgatattt cgttaaggat gcggggacgc
     1741 gtctggcttt tcggcaacga cgtttcca
//
LOCUS       DBNGZA010000474         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2746828, whole genome shotgun sequence.
ACCESSION   DBNGZA010000474 DBNGZA010000000
VERSION     DBNGZA010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2746828"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="AC16FU_10130"
     CDS             complement(<1..1295)
                     /locus_tag="AC16FU_10130"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR2994765.1"
                     /translation="MENSPALKSKDQPAPRKAALKLDTPIQYVKGVGPARAKILAKVG
                     IQAVGDLLYYIPRRYLDRSNIRPIGQLSAVPPDGNRSEEMTVIGKVAAAGILKGRKNR
                     FEVLLKDDTGYLALLWFNWPNYLPKYLKKDMELAVSGQVTYYHGYQMIHPDFEVLSGD
                     GEDELLHTGRIIPLYPSTTDLKAHFLDSRGLRRLVAETLPAVRPVIVDFIPPEVLADV
                     GCPPLDVALNQVHFPDSLADADLARRRLAFDELFFLQLLLQARRATTKKEAKPRTFKP
                     PGDLVWRFLRQLPFQFTAAQKKVFAEITADLTSPAIMNRLLQGDVGSGKTVVAVAAML
                     GAVENGYQAALMVPTEILAEQHYFTLSRRLEQIGVSTALLTGSTPEKEKKEMHKQLAA
                     GKIQIVFGTHALLQEKVTFRDLALVVIDEQHRFGVLQRAG"
     gene            1497..1685
                     /gene="rpmB"
                     /locus_tag="AC16FU_10135"
     CDS             1497..1685
                     /gene="rpmB"
                     /locus_tag="AC16FU_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR2994766.1"
                     /translation="MARICDICGKKPSVGYTVSHANKRSKRTWQPNLQMVRAKVNGKP
                     TRLRVCTGCIGAGKVTKA"
     gene            complement(1888..>2993)
                     /locus_tag="AC16FU_10140"
     CDS             complement(1888..>2993)
                     /locus_tag="AC16FU_10140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR2994767.1"
                     /translation="RDPETKEPTGIFKENAVDLINTVVPPPTKTELTLALEKALVEAR
                     RLGVTSVQEQSDPGDFPGGYEVYREFFDAGKLTLRIDTWLALNDATKPHRLDSLQALF
                     PKAGGWLRAGMLKGFTDGSMGSRTALFFAPYSDDPTTSGIAGYTQDSLNNLVRIGDSL
                     GYQIGLHAIGDKANYMALKAFDFARLTNGPKTRRHRLEHAQVMRPQDIEYCARLGVVA
                     LMQPTHCTSDMRWAEDRVGKERCKGAYAWRSFLDNRVPLAFGTDWPVEPLDPMEGIYS
                     AVTRQPREVVKPLPESWMPEQRITVEEAVRAYTFGSAYAEFQEEKKGSIAPGKLADLV
                     VLSQNIFTIPPREILSTKVDLTIVGGKVVFERK"
BASE COUNT          634 a          857 c          860 g          642 t
ORIGIN      
        1 cccgcccgct ggagcacacc aaagcggtgc tgttcgtcga tcaccactaa agccaggtca
       61 cgaaaagtga ctttctcctg aagcagcgcg tgcgtgccga aaacgatctg gatttttccg
      121 gcggccaatt gcttgtgcat ttcttttttt tctttttccg gcgtcgagcc ggtgagcaaa
      181 gccgtcgaaa caccgatctg ctccagacgc cgcgaaagag tgaagtagtg ctgttccgcc
      241 aaaatctcgg tcgggaccat caaggcggcc tggtagccgt tctctaccgc accgagcatc
      301 gccgccacag ccacgactgt tttcccagaa ccgacatccc cctgcagcag acggttcata
      361 atggcgggtg aggtcaggtc ggcggttatt tcagcgaaga cttttttttg ggctgccgtg
      421 aattgaaagg gtagttgccg cagaaaccgc cagaccaaat cgcctggtgg tttgaacgtt
      481 ctcggtttgg cttccttctt ggtcgtggct cgccgcgcct gcaggagcag ttgcaggaag
      541 aatagttcat cgaaagccag ccgcctgcgg gccaaatcgg catcagctaa cgaatcgggg
      601 aagtgcacct gattgagcgc gacatccaga ggcgggcagc cgacgtcggc taaaacctcc
      661 ggggggatga aatcgacgat gacgggacgg accgcgggca aagtctccgc cactaaccgg
      721 cgcagccccc ggctgtccag aaagtgcgcc ttgaggtctg tggtggaggg atagagcggg
      781 atgatccgtc cggtgtggag gagttcgtcc tccccgtccc cggagagcac ctcgaaatcg
      841 ggatggatca tctggtagcc gtggtagtaa gtaacctgtc cggagaccgc taattccatg
      901 tcctttttaa gatatttggg cagatagttc ggccagttga accagaggag cgcgagatac
      961 cctgtatcgt ccttcaggag tacctcaaag cgattcttcc gccctttcag gatgccagcg
     1021 gcggccacct tgccgatgac cgtcatttcc tcggaacgat ttccatctgg cggcacggca
     1081 ctgagctgcc cgatggggcg aatgttggag cggtcgagat agcgccgggg gatgtagtag
     1141 agcagatcac cgacggcttg gatgccgact tttgccaaaa tcttcgcccg cgcgggaccg
     1201 actcccttca cgtactgaat cggcgtgtcg agtttcagcg ccgctttgcg aggagccggc
     1261 tggtctttcg acttcaatgc tggcgagttt tccatcgccg gggtaacata ccccgctcgc
     1321 tttcggagag tcaagaaaag aggtcgtagg tggaaggtga taggtggctt gtcccgactc
     1381 gtggtgagca aagtcgaacc agcaaagccg aggggtaggg aaacgatttg aaatttccaa
     1441 ttacggactt gccctttgct ccttaatcgc tatattgata gtgtaacgga ggaccaatgg
     1501 cacgcatctg cgacatttgc gggaagaaac cgagtgtcgg ctatacagtc agccacgcga
     1561 ataaacggag caaacgcacc tggcagccga acctgcaaat ggtgcgggcc aaggtgaacg
     1621 gcaagccgac ccgcctgcgg gtctgtaccg gctgcatcgg agcggggaaa gttaccaagg
     1681 cgtagacaaa gactgccgcc tgaatcgaga agacccgcca gcggggcttt ttctattacc
     1741 gagaattgaa gacccctccc tcaccctcct tgatacgagt ctgcgatcct caaggggagg
     1801 gaaattccag ttctcttctt tcctcaaaaa actctctctc cttgtaagag agggcagggt
     1861 gaggtcattt agaaagccaa catctcttta cttcctctca aacaccacct tcccccccac
     1921 tatcgtcaga tcaactttgg tgctcaaaat ctcccgcggc gggatggtga aaatgttctg
     1981 agagagcacg accagatcgg ccagtttccc cggcgcaatc gagccttttt tctcttcctg
     2041 aaattctgcg tacgccgaac caaaagtata cgctcgcacc gcttcttcaa cggtgatgcg
     2101 ctgttcgggc atccagcttt cgggcaaggg cttgacaacc tcgcgcggct ggcgggtgac
     2161 cgcggagtaa attccttcca tcgggtcgag cggttccacc ggccagtcgg tgccaaaggc
     2221 caggggaacc ctattatcca ggaaactgcg ccaggcgtag gcgcctttgc accgttcttt
     2281 ccccacccgg tcttcagccc agcgcatgtc ggaagtgcag tgggtcggct gcatcaaggc
     2341 caccacgcct aaccgggcgc agtattcgat atcctgcggg cgcatcacct gcgcatgttc
     2401 taaccggtgc cggcgcgttt ttggtccgtt cgtcaaccgg gcgaaatcaa atgctttcag
     2461 cgccatgtaa ttggccttat cgccgatggc gtgcaggccg atctggtagc ccaggctgtc
     2521 gccaatgcgc accaagttgt tcagcgaatc ttgcgtatag cccgcaatgc cggaggtagt
     2581 ggggtcgtcg gagtaaggcg caaagaaaag cgccgtgcgc gagcccatcg acccgtcggt
     2641 gaaccccttg agcatgccgg cccgcaacca accgcctgct ttcgggaaaa gggcttgcag
     2701 cgaatcgagc cgatgcggtt tggtcgcgtc gttgagggcc agccaagtat cgatgcgtaa
     2761 ggttaatttc ccggcgtcga aaaattcgcg gtagacttca tacccgccgg ggaaatctcc
     2821 cgggtcggac tgctcctgca ccgaagtaac gcccagccgc cgtgcttcca cgagcgcttt
     2881 ctccaacgcc agcgtcaatt cggtcttcgt cggcggcgga acgaccgtat ttatcaaatc
     2941 aactgcattt tccttgaaaa tgccggtcgg ctctttggtt tccggatcgc gta
//
LOCUS       DBNGZA010000475         1367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_96540, whole genome shotgun sequence.
ACCESSION   DBNGZA010000475 DBNGZA010000000
VERSION     DBNGZA010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_96540"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..735
                     /locus_tag="AC16FU_10145"
     CDS             340..735
                     /locus_tag="AC16FU_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR2994768.1"
                     /translation="MRKLIQRKRNEKGLSLIEVMMSMVIFSIGVLGLAPLIAMAIYGN
                     TYSNEVTNVNLAAQERMEWYRNATIPTPLPFTENLAGVAGKLNVTTRIDDNTTDGTVP
                     AGLFQIHVTVNWTDKNKMPRTMDFDTYRE"
     gene            748..>1367
                     /locus_tag="AC16FU_10150"
     CDS             748..>1367
                     /locus_tag="AC16FU_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR2994769.1"
                     /translation="MQKQFSNQKGMTLVEVLLGALLASMVVASAMEFYASQNNAYLAQ
                     QEVSNMQQNGRVAMDELMGKIRLAGGGLPDGILPIVGKNANPDTIIIRYVGRGGNIDV
                     GDHTQKSQAVPIHVARESDLSAFSVGQVVCLWHQPAGPVEWFTITGLKDNQGIGWVEI
                     QHNGVDFASDPLAGDKVLAMQEFRYFIDNSNALHPTLMRAAQGGAPA"
BASE COUNT          334 a          359 c          435 g          239 t
ORIGIN      
        1 ttccgtaccg gacagaaagt gtaaaagggg agacgggtcg agatgaaaat gcgcaagcaa
       61 atgaagaaag aggaagggcg aatcgttgaa cagtcggaaa cgccggccgc cggaggtatg
      121 gggagcatcc cctaccgtgt tgcggcaaaa ctttgtcccg gtcaaaagct acacagccgg
      181 aggagcggcg gttcttcgtc ccggtttgtt caagcgcttt tgtccctgcc ggttacgagc
      241 gagagaccgg agaaaagaga aaaacgcaca ccgggcaggg cagttgctaa tggttcaggg
      301 gcaggaagaa aacggcaaag gaaagatgag gacctgacta tgcggaaact tatacagaga
      361 aaacggaacg aaaaaggcct gagcttaatc gaggttatga tgtcgatggt gattttcagc
      421 atcggcgttc tgggcctggc gcctttgatc gccatggcga tttacggcaa cacctattcc
      481 aacgaggtca ccaacgtcaa cctggcggcg caggagcgga tggaatggta ccggaacgcc
      541 accatcccca cgccgctccc ctttacggag aacctcgccg gcgtggccgg gaaactgaac
      601 gtgacgacgc gcatcgatga caataccacc gacggcacgg tgccggcggg tttgttccag
      661 atccacgtga cggtgaactg gaccgacaag aacaagatgc ccagaacgat ggatttcgac
      721 acctaccgcg aataagcgag gcgagccatg cagaagcaat tttcgaatca aaaaggtatg
      781 accctggtgg aggttttgct cggcgccctt ttggccagca tggtagtggc gtcggcgatg
      841 gagttctacg cctcccagaa taacgcctac ctggcccagc aggaagtgag caacatgcag
      901 cagaacggcc gggtggcgat ggacgagttg atgggtaaaa tccggctggc cggcggcggc
      961 ctgcccgacg gcatactgcc gatcgtgggc aagaacgcca atccggacac gatcatcatc
     1021 cgctacgtgg gacggggcgg gaacatcgat gtgggcgatc acacccagaa aagtcaggcg
     1081 gtcccgatcc acgtggcccg ggagagtgat ttgagcgcct tttcggtggg gcaggtggtc
     1141 tgcctctggc accagcccgc cggaccggtg gaatggttca ccattaccgg tttgaaagat
     1201 aaccagggca tcggctgggt cgagatacaa cacaacggcg tcgatttcgc tagcgatcct
     1261 ttggccgggg ataaggtttt ggcgatgcag gaattccgct acttcatcga caactcgaat
     1321 gcgctccacc ccactttgat gcgggccgcg cagggcgggg cgccggc
//
LOCUS       DBNGZA010000476        12990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2641096, whole genome shotgun sequence.
ACCESSION   DBNGZA010000476 DBNGZA010000000
VERSION     DBNGZA010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2641096"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..418)
                     /locus_tag="AC16FU_10155"
     tRNA            complement(342..418)
                     /locus_tag="AC16FU_10155"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Met,seq:cat)
     gene            complement(559..810)
                     /locus_tag="AC16FU_10160"
     CDS             complement(559..810)
                     /locus_tag="AC16FU_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR2994770.1"
                     /translation="MFIVYVIQSASGKRYTGHTSDLKHRLEEHNAGLCKSTKIDKEWL
                     VLLAEELANRSEAMKREKWLKAGAGRQFVQKVLAGWSPP"
     gene            complement(991..3075)
                     /locus_tag="AC16FU_10165"
     CDS             complement(991..3075)
                     /locus_tag="AC16FU_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR2994771.1"
                     /translation="MYFLNPFFLFATLFALLPLLIHLFNRQKVKKIEFSSVAFLKAME
                     RTKIRRLKLRQLWLLLLRTLIILLVALAFARPAVRGKFGRKVGSHAKTSAVIILDNSA
                     SMGLETKNGSLFELAQKKTQKIIDLFAEGDETALVLTNGGGQPTLTHNFRRLSRDLAE
                     AQLSSGATALDRTIASAARLLSSSKNLNREIFLFSDLKSPAGAISDTALSHFHGKLFL
                     FPVKAADYENVSLQEIDFGRQLITLGVPFQMTAKVQNQSPRPEEGLLVGLWLDGQKVS
                     QTDLSVPAQKEATVKFTLTVTRPGWHSGWVELPDDDLLADNRRFFAFYLPPQIRVVIV
                     SEAGQPAEHLRLALTAGDSSQSRFAVATIPPEKLLTYDLSPVNVLVFNELKKVPAGFG
                     ARLDSFQKHGGLFVILGTGFDLPSYQSAIFLRFGSVAVESGRDTIPGSGFYTLEQIDF
                     SHPIFQVYQKIPHEKFPQLRFFSVPKVTAGDLTRLASFSNGQPALVEKKAASQPALLF
                     AGPLNPTESDLPRHPFFVPFVQRTVEYLAFSPFAQAEQILVGDTLQRPVDFVYYGKKL
                     VVEDPAKQKLEASAFLTGEQAWVRIEPTDLPGIYQVKAGDDLAAAFAVNLDPRESNQP
                     AWEPASLKRQAPGLGVFTGDPSDPARAILSSRIGKELWKPLLWAAALLLMLEMLVARS
                     AAPREATEFPSS"
     gene            complement(3079..4056)
                     /gene="ilvC"
                     /locus_tag="AC16FU_10170"
     CDS             complement(3079..4056)
                     /gene="ilvC"
                     /locus_tag="AC16FU_10170"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR2994772.1"
                     /translation="MRAFYDSDADLSLLKGKTVAVIGYGSQGRAQALNLRDSGAAVVV
                     GLPQTSASRKNAREDGLAVSSTEEAGKRGDFISLLVPDQLHQKIFQKSIRPYLTPDKT
                     LVMAHGYSVHFRLIQPPTTVDTLLVAPLGPGEILRERFTRGKGIACLLAVRSKNQTSA
                     WTKAKTYARAIGCTRMGAIATTFREEAVGDLFGEQAVLCGGLSQLLLAGFETLVKNGI
                     NPESAYLECVQQIDLIMALIKRHGLRGMFQRISATAAYGAARSGRKVIDHRVRRSMQQ
                     IFTDIASGRFARNWQKTFTDRHKVERLLETASAPVFERAHKRVQALLRE"
     gene            complement(4117..5337)
                     /locus_tag="AC16FU_10175"
     CDS             complement(4117..5337)
                     /locus_tag="AC16FU_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994773.1"
                     /translation="MAIAWSEALGVSLGALRANRIRSFLTMLGIIIGVGAVITMIALG
                     QGAKKAVQDRIESLGANLLFVRPGAPFRGGVRFESGSNVRLTDKDVEAIQTKVRGLEI
                     MVPETGRRAQVKYGNQNWNTQVSGTIPEYQFVRNSPCMVGNYFTKNDVATRQRVAVLG
                     HEVWNNLFNGQDPVGKEVRINNISFTVVGLLAEKGQAMMFNQDDQILIPITTAQKRLF
                     GIDFLTNVNVKVADEAVMEQAALDIEQVLRRQHRLKPGEENDFNIRSQADILSTLSET
                     SQTFTTLLASIALVSLLVGGIGIMNIMLVSVTERTREIGTRIAIGAKKRDIMNQFLIE
                     ALLLSLCGGAIGAAAGIGGSVLLSKLANWNTLISVSSIFLAFLFSAAVGIFFGLYPAR
                     KAARLNPIEALRYE"
     gene            complement(5389..6768)
                     /locus_tag="AC16FU_10180"
     CDS             complement(5389..6768)
                     /locus_tag="AC16FU_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994774.1"
                     /translation="MKKWIWIIVGVVAIAAVVVYAATRQNGKNDKTQSATGKIVKPFR
                     GDLVVSITATGQIEPIDAVEIRSKASGEIIRLPIDDGVSVKKGDLIAQLDQTTAQNDY
                     EQAKADFGVAEATLNQREKELNRVKGLFEKNLVSQQDLDNAQLAYEQARSQFVRTKTT
                     LSTMEERLKDTVVRSPISGVVLQKNVEAGQIISSAVSSVSGGTLIATVAGMDKVYVVA
                     DVDETDIGKVAIGQGAKVVADAYPDEEFHGRVLKIAPLAKVAQNVTTFDVTAEVDNAN
                     HLLKAGMNANVDIVTAEARNALLVPVEALVEPAEAKMAMGNGAPGPGQSAEAQTKPPA
                     DAGGQTREISPERRERFRQMMESGQVTPEMMAQWQKRRAARRDTSAVNPEKPHARTKV
                     VYVLEKGQPTPRKVTVGLSNLDNAQIVDGLTEQDEIVVQSSSQLMRDREEMKQRMQRF
                     TGLPGVKKQ"
     gene            complement(6854..7330)
                     /locus_tag="AC16FU_10185"
     CDS             complement(6854..7330)
                     /locus_tag="AC16FU_10185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR2994775.1"
                     /translation="MFYRRGKRQRILLLKRSAGSNWGGLWQGVSGKIKKGESAIRACL
                     REIGEETNLSVKRLYALDLVNQFYAPRTDTVYLGPFFAAEVTTPESLRLSREHDACRW
                     ATVQQALRLVIWEGYRRALLRVQNDLFSKNAVDRNFLVYEPPESKQKGSKARLLRI"
     gene            complement(7397..9385)
                     /locus_tag="AC16FU_10190"
     CDS             complement(7397..9385)
                     /locus_tag="AC16FU_10190"
                     /inference="COORDINATES: protein motif:HMM:NF020086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HIR2994776.1"
                     /translation="MSPRAKKTAAKTDPTTVRPLPMTPEELHLRGWDSLDILLITGDA
                     YIDHPAVHVALIGRLLESHGFKVGVIAQPDWKNLSDFSRLGRPRLAVYVTGGQNEPLI
                     THFLAKDKPLLPDAWSPGGSPVKRPARAVMTYAQRARQAFPGLPVIIGGVEASLRRLA
                     YFDPWNNAVRRSILFDAKADLLIYTPGEFEMLAVAQRLAQGEPIEKITNLPGTTVKRR
                     RLPSRGPVRELFSYDEVKTDKLKYLEAFSDQAVQRAPGAGFPVAQLTGDRYVVVNPPP
                     ARTTAELDLLYALPFTRLPHPSYGEREKIAVAEALRFAIVSHRGCVCGASFCQHEPYQ
                     GSQIQSRSEQSILDEAVSFASDPAFQGMISDLSGPYANHYGSDFISGNGQNQSRTVFA
                     EQGGSDGDGNGGGSTAALTDLRAAEMPFGAQQAFTLLLQRLQKLPGINRVLLTSPLRL
                     EWAAADPDFLNQVATHHVQNHCRIMPGHVTKAVNRLMRRPGVEMLDKFLALFLDLSQK
                     AKKEQFIQAHFIINHPGCRTFDMVELAAYSRKRRLTLNWFWDFSPVPLTASGVMAYTA
                     LDPETRQPLYVPREAKDRLIQKALVRYLDPGSHYLVKKALYACNRADLIGSSSLCLIS
                     PKPKPRVMRKPVQARPDTHLPPRPAGTNRLDAFRHRSY"
     gene            complement(9389..10096)
                     /gene="bshB1"
                     /locus_tag="AC16FU_10195"
     CDS             complement(9389..10096)
                     /gene="bshB1"
                     /locus_tag="AC16FU_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR2994777.1"
                     /translation="MKRKEFDVIAIGAHPDDVEVGTGGVMIKLHQLGYRTGLVSLTRG
                     EMGTGGTAAIRRKETRRGAAILGAQLLATLNFGDCKLVDSFTNRLKVARLLRKFRPRV
                     VLAPFWDGGHGKRQGHPDHLACGRIVMNAVNFAGLKKMPLREEPHQVKALFHYFLPAG
                     LMPTFVVDITAEFPRWREALKAHRSQFLNPEKSLDYLWFLESMARTYGNYIRTRYGQG
                     FVIGEPFKIDDLTLLAG"
     gene            10239..11336
                     /locus_tag="AC16FU_10200"
     CDS             10239..11336
                     /locus_tag="AC16FU_10200"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR2994778.1"
                     /translation="MKYVAAAAAISRRRGPAILLFTSFPPGIILQMSVLLATLKKYQR
                     IWGPLAALLFLALCVYDLDFNLVWSAIQRVHYGYLVPSVFSCFIFALLRSQRWRMIID
                     PRKPISRRRIFRLFMIGQLANAALPALTGQAVRIWLLSKKEGLTKTFAFTTVVLEVVF
                     DALSLLLLLAGFSSLLVLPRWLVRGELAVALGITVLFVLFYFFLRERPPVWMEKYLIT
                     IPAHRVKKLNDLYHSVSSGLAMLKSTRHLFSVFGLSLLAWLAQTGTVFFLMEAFGLEA
                     PLEAALLVIVINTVALIIPITPGNVGTFQLACTFALSLFAVPKAEAFSFSVLLQVVDL
                     LPVVTLGVYFLVAEHLRVRDLKSAEVLEYRP"
     gene            11476..11976
                     /locus_tag="AC16FU_10205"
     CDS             11476..11976
                     /locus_tag="AC16FU_10205"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR2994779.1"
                     /translation="MVAVKELMLNNVNVVNVGYMAPTFTLLDQKGTACTLLDEMGRHH
                     VVLVFCRGSWCPASAQHLIALNQVQNEFAQRGAVVWAVSAERQRDTQRFAKKEKINFP
                     LLEDKNLEVITRWGVRSSNSARPVPHPATFLIDIDGIVRLKEVSVNEVRYSPPSRLIE
                     QLALLA"
     gene            12123..>12990
                     /locus_tag="AC16FU_10210"
     CDS             12123..>12990
                     /locus_tag="AC16FU_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994780.1"
                     /translation="MTLDDALGQAKEKNESYLIAQQEIKKARAQIREARSGALPTLTL
                     SSTYTRNWQLPAAVFDIKGERQRIRLGADNSYLANLTLQQPLYVGGKVGAALKIARYY
                     EAVVKARAREVEAALELEVKRAFYDAVLAQDLVTVAQEALRQAESNRDVVAKLFKEGM
                     VAEFELLRAEVQVANLKPQLLKARNGADLALHNLKTVIGAPLAQEIALVFQFPQARVA
                     RVYDYDSLLAQALALRPEVKQLEDQVKIQSQLIGIAQGGYRPSLFASTSYQFQAQSNR
                     FVPDGRDWIHSWN"
BASE COUNT         2656 a         3685 c         3763 g         2886 t
ORIGIN      
        1 aaatcgatac cctgtgctga ttttaatgat gaattgagat gcacttggtg aaagatcaac
       61 atgtttatga gaaaaatgcc gtgccccact tgagatttgg acaggtccaa ttgtgtaagg
      121 aggattaatg aagaacgctt catcaggggg atttaggcag gtatcaatac cctcaaattt
      181 taagcacttt ttcgatgaat tggacggagg ggttcacaaa tcgtttggtc gggcatccgc
      241 tatggacaaa tttcgcaccg gatggttcgc acatcgttta actccaacta ccccgctatg
      301 aacaggacga caaccagtaa ctgattgtcg cccttcgatt ttggtagcgg gggttggatt
      361 tgaaccaacg accttcgggt tatgagcccg acgagctacc ggactgctcc accccgcgtc
      421 aaaggcaaga tacgatcttt tctcggctga gtcaaggcgt ccacgtaatc tcggtgaaac
      481 gtttgtaagc gtgtcgtgga ttcgtcgaca gacccgaatc tgcctgaggc ggattatgag
      541 cccgacgagc tactccgcct atggcggact ccaccccgcg agtacttttt ggacaaactg
      601 acgaccagcc cccgccttca gccacttttc tcgtttcatt gcttcagaac gattggctaa
      661 ctcttcggct aaaagaacca gccattcctt atcgattttg gtgcttttac aaagtccggc
      721 gttgtgttct tccagtcggt gtttcagatc agaggtatgc ccggtataac gtttaccgga
      781 agccgactga ataacgtaaa cgatgaacat gaagaagtgc taaaaaaata gcgggggcgc
      841 gatttgaatc cgcctgtggc gggattatga gcccgacgag ctactccccg ccgcggcggg
      901 tggcggactc caccccgcgt cgagggcatg atagcatctt ttcctggcgg cgtcaaggag
      961 tccagtcaca cagccccggc ggaacgtttg tcagcttgaa ggaaattcag ttgcttcccg
     1021 cggagcggcg ctgcgggcta ccagcatttc gagcatcagg agcagcgccg cggcccagag
     1081 cagcggtttc cagagttctt tcccgatgcg gctggacaga atggcgcggg cggggtcgga
     1141 cggatcaccc gtgaagacgc ccagtccggg tgcctgacgc tttagagagg ccggctccca
     1201 ggccggttgg tttgactccc ggggatcgag gttgaccgca aaggccgccg ccagatcgtc
     1261 acccgctttc acttgataaa tcccgggtag atccgttggc tcaatccgca cccaggcctg
     1321 ttcgccggtg aggaaggcgc tcgcttcgag tttttgcttg gcgggatctt ccactaccag
     1381 tttcttgcca taataaacaa aatcgaccgg gcgctggagc gtgtccccca ccagaatctg
     1441 ttccgcttgc gcgaaggggg agaaagccag gtactcaacg gtgcgctgta caaaggggac
     1501 aaagaagggg tgccggggga gatcgctttc cgtcgggttc aaggggccgg cgaaaagaag
     1561 agccggctgg cttgccgctt tcttttccac cagggcgggc tgaccgttgc tgaaactggc
     1621 cagacgggtc aggtcgccgg cggtaacttt gggcacgctg aaaaagcgga gttgcggaaa
     1681 tttctcgtgg gggatcttct gataaacttg aaagattggg tgggaaaagt caatctgctc
     1741 cagggtgtag aaacccgaac cggggatggt atcccggccc gactctactg cgacgctgcc
     1801 aaaccttaaa aaaatagcac tctggtagga cggcagatcg aaccccgtcc ccaagatgac
     1861 gaaaagtcca ccgtgcttct ggaaggaatc aagccgcgca ccaaatccgg caggcacttt
     1921 tttcagttca ttgaagacta aaacgttcac ggggctgagg tcgtaggtca ataacttctc
     1981 cggggggatg gtggccacgg caaaacggct ctggctggaa tctcccgcgg tgagggccag
     2041 gcgcaggtgt tcggcgggct ggcccgcctc ggaaacaatc accacccgga tttgcggtgg
     2101 cagatagaag gcaaagaagc gccggttgtc ggccaagagg tcgtcatcgg gcagttccac
     2161 ccacccgctg tgccagccgg gacgggtgac ggtcagcgtg aacttgaccg tcgcctcctt
     2221 ttgcgccggc accgagagat cggtctggct caccttctgt ccgtcgagcc agagaccgac
     2281 caagagacct tcctccgggc gcggactctg gttttggact ttggcggtca tctgaaaggg
     2341 cacgcccagg gtgatcaact ggcggccaaa atcaatttcc tggagactta cgttttcgta
     2401 atcagcagct ttgacgggga agagaaacag tttgccgtgg aaatgggaca aggcggtgtc
     2461 ggaaattgct ccggcgggac ttttcagatc agagaagagg aaaatttcgc ggttcaaatt
     2521 tttcgaagat gaaaggagtc gggcggcgga agcgatggta cggtcgaggg cggtggcgcc
     2581 ggaggaaagt tgggcctccg ccaggtcgcg ggagagccgg cggaagttat gcgtcagcgt
     2641 cggctggccg ccgccattgg tcaaaacgag cgccgtttcg tccccttcgg cgaacaggtc
     2701 gatgatcttc tgtgtctttt tctgcgccaa ttcgaacaga gaaccattct tggtctccaa
     2761 gcccatactg gcggagttgt cgaggatgat caccgccgac gttttggcgt gcgagccgac
     2821 cttccgcccg aacttccccc gtaccgccgg acgggcaaag gcgagcgcca ctaagagaat
     2881 gatgagcgtg cggaggagga gaagccagag ctgccgcagt ttgagccggc ggattttggt
     2941 ccgctccatg gctttcagga aagccaccga actgaactcg attttcttca ccttctgccg
     3001 gttgaagagg tgaattaata gcggcaaaag agcgaagagc gtcgcgaaga ggaagaacgg
     3061 gttgaggaag tacatcggtt actcgcggag gagggcctga acgcgcttgt gggcacgttc
     3121 aaaaacaggc gccgacgctg tctccaaaag cctttcaact ttatggcgat ccgtaaacgt
     3181 tttctgccag ttgcgggcga accggccgga agcaatatcg gtgaaaattt gctgcatgga
     3241 acgccgcacg cggtggtcta tcactttccg tccgctcctg gcggcaccgt aagccgcggt
     3301 ggcggagatg cgctggaaca tgccgcgtaa gccgtgccgc ttgatcaacg ccataatgag
     3361 gtcgatctgc tggacacatt ctaagtatgc gctctccggg ttgatgccgt ttttcaccag
     3421 cgtttcaaac cccgccaaaa gtaactgaga taaccctccg cagagtaccg cctgctcgcc
     3481 gaataaatcg cccaccgctt cctcgcggaa cgtggtggca atggctccca tacgcgtgca
     3541 gccaatggcc cgggcgtagg ttttggcttt cgtccaggca gaggtttggt ttttcgagcg
     3601 caccgccaaa agacaggcaa tgcctttccc ccgggtgaaa cgctcgcgca gaatttctcc
     3661 cggacctaaa ggggcaacga gaagcgtgtc aacggttgtg ggcggttgaa tcagccggaa
     3721 gtggacgctg tatccgtgcg ccataaccaa agttttgtcc ggcgtcagat atggccgaat
     3781 tgacttttga aaaatctttt ggtgcagttg atcgggaacc aaaagagaaa tgaaatcgcc
     3841 tcgcttgccg gcttcttcag tggaagagac cgccaggcca tcttcccgcg cattttttct
     3901 tgaggcgctg gtctgcggca gtcccacgac aaccgctgcg ccgctgtcgc gcaaattcag
     3961 cgcctgcgcc cggccctggc tgccgtagcc gatgacggcg acggttttgc ctttcaaaag
     4021 cgaaagatcg gcgtcgctat catagaacgc acgcatagag acaatatacg tggttcaatg
     4081 gaaaatctct cgtttggatt ctgaaagagg gagttactac tcgtagcgca gcgcttcaat
     4141 cgggttcagg cgagcagctt tgcgcgcggg gtagaggccg aagaaaatgc ccaccgccgc
     4201 cgaaaagagg aacgccagga aaatcgaact gaccgaaatc aacgtgttcc agttggccag
     4261 tttcgatagc agcaccgagc cgccgatgcc ggccgccgcg ccgatggcgc cgccgcagag
     4321 ggaaagcaaa agcgcttcaa tcaaaaactg gttcataatg tcccgctttt tggcaccgat
     4381 ggcgatgcgc gttccgattt cccgggtcct ttcggtaacc gagacgagca tgatgttcat
     4441 aatcccgatc ccgccgacca aaagcgagac caaagcaatc gaagcgagga gggtggtgaa
     4501 ggtttgggac gtttcgctta aagtcgagag gatatcggcc tggctgcgga tgttgaagtc
     4561 gttctcctcg cccggcttca gccggtgctg gcggcgcagc acctgctcga tgtccaaggc
     4621 cgcctgttcc atcaccgctt cgtcggccac tttcacattg acgttggtca gaaagtcaat
     4681 tccgaagagc cgcttttgtg cggtggtgat ggggatcaaa atctggtcgt cctggttgaa
     4741 catcatcgcc tgcccttttt cagccaacag tcccactacg gtgaaggaaa tgttattgat
     4801 gcggacttcc ttgcccaccg ggtcttgtcc gttgaacagg ttgttccaga cttcatgtcc
     4861 taagaccgcc acgcgttggc gggtggccac gtcgtttttc gtgaagtaat ttcccaccat
     4921 acaaggagag ttgcgcacga actggtactc gggaatggtg ccgctaacct gcgtgttcca
     4981 gttctggttg ccgtacttga cctgcgcccg gcggccggtc tcggggacca taatttctag
     5041 cccgcgcacc ttggtttgaa tcgcctccac atccttgtcg gtcaggcgaa cgttggagcc
     5101 agattcaaag cgcaccccgc cgcgaaaggg cgcgcccgga cggacgaaga gcaaattggc
     5161 ccccagggat tcaatccgat cctgcactgc tttcttcgcg ccttgcccca gcgcgatcat
     5221 ggtgatcacc gctcccacgc cgatgataat ccccagcatg gtcaaaaacg agcggatccg
     5281 gttggcgcga agggcgccta aagagacacc gagggcttca ctccaggcta tggccatagg
     5341 tctatttgcg ttgctttgat cctggttttg tttatgccag ctcccggact actgtttttt
     5401 cactcccggc agtcccgtaa atctttgcat cctttgtttc atttcctcac ggtcgcgcat
     5461 taactggctg gaggactgga cgacgatctc atcctgttcg gtgagcccgt cgacaatctg
     5521 ggcattgtcc agattcgaaa gtcccaccgt caccttccgc ggggtcggct ggcctttttc
     5581 caaaacgtag acgactttag ttcgggcgtg cggcttttcc gggttcaccg ccgaggtatc
     5641 ccggcgcgcc gcgcgccttt tctgccactg ggccatcatc tccggggtga cctgcccgct
     5701 ctccatcatc tgacggaagc gctcgcgccg ttccggtgaa atttcgcgcg tttggccgcc
     5761 cgcgtcagcg ggtggtttag tttgggcttc tgcagattga cccgggccgg gagcaccgtt
     5821 ccccatggcc attttggctt ccgccggctc caccagcgcc tccaccggta ccaaaagcgc
     5881 gttccgggct tcggcggtaa cgatgtcaac gttggcgttc atcccggctt tgagtaagtg
     5941 gttagcgttg tctacctcag cggtgacgtc aaaggtggtt acgttctggg cgactttagc
     6001 caatggtgcg attttgagca cgcggccgtg gaactcttcg tcggggtagg cgtcggccac
     6061 gactttggct ccttggccga tggccacctt accgatgtcg gtctcatcca cgtcggccac
     6121 gacgtagact ttgtccatac ccgcgacggt ggcaatcaag gttccgccgg aaacggacga
     6181 gaccgccgag gagataatct gtcctgcttc gacattcttc tgcaggacga ccccagagat
     6241 gggcgaccgg accaccgtgt ctttcaggcg ctcttccatc gtggagagcg tcgtcttggt
     6301 ccgcacgaac tgcgaccgcg cctgctcata agccagttgg gcattatcca gatcctgctg
     6361 cgagaccagg tttttctcaa acagcccctt cacccgattc aattcctttt cgcgctgatt
     6421 caaagtcgcc tccgccacgc cgaagtcggc cttcgcctgc tcatagtcgt tctgcgcggt
     6481 ggtctggtcc agttgggcga tcaggtcgcc ctttttgacc gacacgccgt cgtcgatggg
     6541 caggcggatt atttcacctg aggctttcga tctaatttcg acggcatcta tcggctcgat
     6601 ctggccggta gcggtgatgg aaaccaccag gtcgccccgg aacggtttga caatttttcc
     6661 cgtggctgat tgagttttgt cgtttttgcc gttctgccgg gtggcggcat aaacgaccac
     6721 ggcggcaatg gccacgaccc cgacgataat ccatatccat tttttcattc aatcctcgcc
     6781 tgaaaattgt gcactgtctt ttcgcttttg gatacgttgg ttctcccgaa atcgattggg
     6841 ttcgctctct ttactatata cgcagcagcc gcgctttcga tcccttctgc ttactctcgg
     6901 gaggctcgta cacgaggaag ttccggtcga ccgcattttt cgaaaagaga tcgttttgga
     6961 cccgtagcag cgcccgacgg tacccttccc aaatcaccag tcgcaacgct tgttgtacgg
     7021 tagcccagcg gcaggcgtca tgctcccggc tcaggcgcaa agattctggc gtggtgactt
     7081 cggcggcgaa aaaggggccg aggtagacag tgtcggtgcg cggtgcatag aactggttca
     7141 ccagatcgag agcgtagagg cgtttcacgc tcaaattcgt ctcttctccg atttcccgca
     7201 agcaggcccg gatggccgac tcaccctttt tgatctttcc cgagaccccc tgccagaggc
     7261 cgccccagtt tgaaccggcg ctccgtttca aaaggagtat cctttgtctt ttaccgcggc
     7321 ggtagaacaa aaatacctca acgagatcgc cttttatttt agacacaggg ggtgagacaa
     7381 aggtttaccg gctgtctcag tacgaccggt gccggaaggc gtcgagccgg ttcgtcccgg
     7441 cgggacgcgg agggagatgt gtatccgggc gtgcctgcac cggtttgcgc atcacccgcg
     7501 gtttgggctt ggggctgatg agacagagcg aactggagcc gatgaggtcg gcccggttgc
     7561 aggcatagag tgctttcttc accaggtaat ggctgccggg atccaggtaa cgcactaagg
     7621 ctttctgtat cagccggtcc ttcgcctcgc ggggaacgta caaaggctgg cgggtctcgg
     7681 ggtcaagcgc ggtgtacgcc atcacccccg aggcggtgag cggtacgggt gagaaatccc
     7741 agaaccaatt cagggtcagg cgccttttgc ggctgtaggc cgccagttcg accatatcaa
     7801 aagtccggca gccgggatga ttgatgatga agtgcgcctg gataaactgt tcttttttgg
     7861 ctttctgcga gaggtccaga aagagggcca gaaatttatc cagcatttcc acgccgggcc
     7921 gccgcatcaa acggttcacg gccttggtga cgtggcccgg cataatccgg cagtgattct
     7981 gaacgtgatg ggtggctacc tggttcagga aatcggggtc ggccgcggcc cactccagcc
     8041 ggaggggtga ggtcaaaagc actcggttga tccccggaag tttttgcaat ctctgcaaga
     8101 gaagggtgaa agcctgttgt gcgccgaagg gcatttccgc agcgcgcagg tcggtcaagg
     8161 ccgcagtcga tcctccgccg ttcccatcgc cgtctgaccc cccctgctcg gcgaatacgg
     8221 tccgggattg gttctgtccg tttccggaaa tgaaatcact gccgtagtgg ttggcgtacg
     8281 ggccactcaa gtcggaaatc atgccctgaa aggccggatc ggaggcgaac gaaaccgctt
     8341 catctagaat tgactgttca ctgcgtgact ggatctggct gccctggtac ggttcgtgct
     8401 ggcagaaaga ggcgccgcaa acacagcctc gatggctaac aatggcaaag cgcagtgcct
     8461 cggccacagc aattttctcc cgctcaccgt acgacggatg cggcaagcgg gtgaaaggta
     8521 aggcgtaaag gagatccagt tcggcggtgg ttctggcagg cggcggattg accactacgt
     8581 accggtcgcc ggtcaactgc gctacgggaa agccggctcc gggcgcccgc tggaccgcct
     8641 gatcgctgaa cgcctcgaga tatttcaatt tgtcggtttt cacttcgtcg tatgaaaaaa
     8701 gttcccgtac cggtcctcgg gaagggagcc gtctccgttt cactgtagtg ccagggaggt
     8761 tggtgatttt ctcgatcggc tcaccttgcg ccagacgctg ggccaccgcc agcatttcaa
     8821 attctcctgg cgtgtaaatc aaaaggtcgg ccttggcatc gaagagaatg gagcgccgca
     8881 ccgcgttatt ccaggggtca aaataagcca gccgccgcag ggaagcttcc accccgccga
     8941 tgatgaccgg taagccgggg aaagcctggc gggccctttg cgcgtaggtc atcaccgccc
     9001 gggccggccg cttgaccgga ctgccacccg gcgaccaggc gtcgggcaaa agcggtttgt
     9061 ctttggccaa aaaatgggtg atgagcggtt cgttctgccc gccggtgacg tagacggcca
     9121 gacgcggtcg gcccaaacgg gagaaatcgg aaagattttt ccaatcgggc tgggcgatga
     9181 caccgacttt gaacccatgc gattccagga gccgcccaat gagtgccacg tggaccgcgg
     9241 gatggtcaat gtaggcgtcg cccgtgatca atagaatgtc gaggctgtcc caacctctga
     9301 ggtggagttc ctcgggcgtc ataggcaagg gccggactgt cgttggatcg gttttcgccg
     9361 cagttttttt ggctctcggt gacataggtt atccggcgag gagggtgagg tcgtcgattt
     9421 tgaacggctc tccaataaca aatccttgac cgtaccgggt acgaatataa ttgccgtagg
     9481 tacgggccat tgattccaaa aaccagaggt aatccagaga tttttccgga ttcaaaaatt
     9541 gcgaacggtg ggccttcagg gcttcccgcc accgcgggaa ttcggcggtg atgtccacca
     9601 cgaacgtagg catcaacccc gccggaagga agtaatggaa aagcgccttc acctggtgtg
     9661 gctcttcccg cagcggcatt tttttcagtc ccgcgaagtt gaccgcattc atgacaatcc
     9721 ggccgcaggc caggtggtcg ggatgtccct gacgtttgcc gtggccgccg tcccaaaagg
     9781 gagctaagac gacccggggc cggaactttc gcaaaagccg ggccaccttc agtcggttag
     9841 taaaagaatc gaccagtttg caatccccga agtttaacgt ggccaaaagt tgagcaccca
     9901 ggatggcggc gccccgccgg gtttcctttc tgcggatagc cgcggtgccg ccggtgccca
     9961 tttcgccccg ggtgagggag acgagaccgg tccggtagcc cagttggtgc aacttgatca
    10021 tgaccccgcc cgtgcccact tccacatcgt ccggatgagc accgatggca atgacgtcaa
    10081 actctttccg cttcatcctt ctttattctg gtgccccttg acaacccact tttcgctttt
    10141 accatccctg gtagccccgc agggtaaaaa gaatatacac cgtcccaccc gagatgcaag
    10201 agaaatcttt cggtactatc gcgccgaaag atgagcggat gaaatacgtc gccgccgcag
    10261 ccgcaatcag ccgacggcgg ggcccagcta ttttgctctt tacttcattc ccgccaggta
    10321 tcatcttgca aatgtccgtg ctcctggcga ctttgaaaaa gtaccagcgg atctggggcc
    10381 ccctggcggc gctcctcttt ttggccctct gcgtctacga tctggatttc aacctggtct
    10441 ggtcggcgat ccagagggtg cactacgggt acctcgtgcc ctcggttttc tcttgtttca
    10501 ttttcgccct cctgcgcagt cagaggtggc ggatgatcat cgatccccgc aagccgatct
    10561 cccggcgccg cattttccgg ctcttcatga tcggccagtt ggccaatgcc gccctgccgg
    10621 ccctcaccgg ccaggcggtg cgcatctggc tcttgagcaa aaaagagggc ctgaccaaga
    10681 ccttcgcttt caccaccgtg gttctggaag tggtcttcga cgccctgagt ctccttttgc
    10741 tcctcgccgg attctcgtcg cttctggtcc tgccccgctg gctcgtgcgg ggtgaactgg
    10801 cggtggctct gggcatcacc gtcctttttg tcctctttta ctttttcctg cgcgaacgac
    10861 ccccggtctg gatggagaag tacctcatta ccatcccggc gcaccgcgtc aagaagttga
    10921 acgatctcta ccactccgtg tccagtggtc tcgccatgct caagagcacc cggcacctct
    10981 ttagtgtgtt cgggttgtcg cttttggcct ggctggcgca gaccggcacg gtcttctttt
    11041 tgatggaggc cttcggtctg gaggcgcctc tggaagcggc gcttttagtc attgtcatca
    11101 ataccgtggc tttgatcatt cccataaccc cgggcaatgt cggaaccttc cagttggcct
    11161 gcacctttgc gctctccctt tttgcggtgc cgaaagcgga agcgttcagt ttctcagtct
    11221 tactccaggt cgtggacctt ttgccggtgg tgaccctggg ggtctatttt ctggttgccg
    11281 agcacctgcg ggtgcgcgac ctgaagagcg ccgaagtatt ggagtaccga ccgtaaacgt
    11341 ttgcctgctc cagacccgca cgcaggcgcg gcgcctcgcc gcgtgcgctg gtggcaccgg
    11401 aggcttgacg gtattggaac agggcgcata ttggtatggc ccaataagga atgtagcttt
    11461 tccaagaggc agaggatggt tgcagtcaaa gaacttatgc tgaacaacgt caatgttgtc
    11521 aacgtcggct acatggcccc aaccttcaca cttttagatc agaaaggaac ggcgtgcacc
    11581 ttacttgacg aaatggggag acaccacgtc gttttagtct tctgccgcgg ttcctggtgc
    11641 ccggcctccg ctcaacattt aatcgccctt aaccaggtgc agaacgagtt tgcccagcgc
    11701 ggcgcagtcg tctgggcggt atccgccgaa aggcagcgcg acacccagag gtttgcgaag
    11761 aaggaaaaga taaacttccc acttttagaa gacaaaaacc tggaagtaat aacccgctgg
    11821 ggagtaagga gttccaactc cgcccggccg gtgccccatc cggcgacctt tttgatcgac
    11881 attgacggca tcgtcaggct caaggaagtt tccgtcaatg aagtccgcta ttcgccgccg
    11941 agtcgtctca tcgagcagtt ggccctttta gcgtagctta tccagacaaa cacagaacct
    12001 tattttgcgt agggattttt gtgaaattat tcactaatat gatatgggtc ataagggcat
    12061 ttttcactat gagtgctggg gtaatttttt tgtccctttc aacccttgcc gagcctttac
    12121 ctttgacctt ggatgacgcc ctcggccagg ccaaagagaa aaatgaatcc tacctgattg
    12181 cacagcagga aatcaaaaaa gcccgggcgc agatccggga agctcgctcc ggcgccctgc
    12241 cgactttaac gctctccagc acttacaccc gcaactggca gttgccggcc gcagtctttg
    12301 atatcaaagg ggagcgacag agaatccgct tgggggcgga caatagctac ctcgccaacc
    12361 tgaccctcca gcagcccctc tacgtcggcg gcaaagttgg ggcggcgctc aagattgccc
    12421 ggtactacga ggccgtggtc aaagcccggg cccgcgaggt cgaagccgca ctggaactcg
    12481 aggtcaagcg ggccttttac gacgcagttc tggcgcaaga cttagtcacc gtggcgcagg
    12541 aagccttgcg ccaggccgag tcgaaccggg atgtagtggc caagttattt aaggaaggaa
    12601 tggtggcgga gttcgaactc ttgcgggccg aggtgcaggt ggccaacctc aaaccgcaat
    12661 tgctcaaagc ccggaacggc gccgatttgg ctttgcacaa tctcaagacg gtcatcggcg
    12721 cgcccttggc acaagaaatt gctttagtct tccagttccc ccaagcgcgg gtcgcgcggg
    12781 tttacgacta cgactcactt ttggcgcagg ctttggcgct ccggcccgaa gtgaagcaat
    12841 tagaagacca ggtcaaaatt caaagccagc tgatcggcat cgcccagggc ggctaccgcc
    12901 cgtcgctttt cgcctccacc tcctaccagt ttcaggcgca atcgaaccgg ttcgtcccgg
    12961 atggccggga ctggatccac tcctggaaca
//
LOCUS       DBNGZA010000477         4922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_200160, whole genome shotgun sequence.
ACCESSION   DBNGZA010000477 DBNGZA010000000
VERSION     DBNGZA010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_200160"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..279
                     /locus_tag="AC16FU_10215"
     CDS             25..279
                     /locus_tag="AC16FU_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994781.1"
                     /translation="MRSKQISLATVSLLVTVAGWLALDCARKIQNPVGLPPGLGVPGE
                     TTYVQIAPAWTSAGGVAFSHPHDVIIGQDLFIYVADTDND"
     gene            436..1110
                     /locus_tag="AC16FU_10220"
     CDS             436..1110
                     /locus_tag="AC16FU_10220"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HIR2994782.1"
                     /translation="MDTVITQQGTIFKRFFTSGGDFIPVYTAPALPPSGGFSVCQPTI
                     FAGIAASPFADREYFISDLTRRKVIAFDRLNQPDTLGIATAGGRGVNSPLGLAAQPVG
                     QTWRLGMVEGRDAQVFRNEDTVFTAVYACSTGQELICVGQFQFATAITFDDLGNWFVA
                     DSGANEVAKFDRNGNFVLRFGRTGSGDGEFNGPAGIAFYDRVVYVADSGNSRIVRYVL
                     NTDIRP"
     gene            1650..1732
                     /locus_tag="AC16FU_10225"
     tRNA            1650..1732
                     /locus_tag="AC16FU_10225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1684..1686,aa:Leu,seq:cag)
     gene            1842..3422
                     /locus_tag="AC16FU_10230"
     CDS             1842..3422
                     /locus_tag="AC16FU_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2994783.1"
                     /translation="MRTKMKVVAFAAVASSLLFLSPILAQRPLSQKEKELYYGIKAVA
                     APEEFKQFQKLSGDKRAEWLRIFWVSRDPTPTTPENEFYNEHQRRVAYAVEHFHTNRL
                     GYLWDDRGEVYIRFGEPDERNFTLERHYDRQQQWHSAYDLRDVLNNPLKLRMLMARQN
                     FDPEGGFGGQNTGADSGGYGPPSAADIKTELENAGKDFDTFSGEVWRYYRYGFTFQFQ
                     DDRNLGYYELVPYVDESGRGQDFQEFVRQAVVSTEIQKEIYRHDYGGQAMDYAFTVTR
                     FRGDNKVNYVVDVNLGLPLDRLGVAGESASGGQVECMRRIVLYDENYEPVASDSTTLV
                     APVSAENRKGQLLIEQKRYEVKPGKYTLAVEVRDLVTQRIGIYKKELLLPEYIAPDVQ
                     EISDVELASYIRPASDWDKRYRHKGLVVMPQPSRVFFPDQVMQYYFEVYNLKKAKDGK
                     ARFSIRADVYGYRTRKVEYTFNPRDFEADTTDIYQTGKINLRDLAPGQYILSLKVKDY
                     TTGKEKTALAGFKIARSE"
     gene            3580..>4922
                     /locus_tag="AC16FU_10235"
     CDS             3580..>4922
                     /locus_tag="AC16FU_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR2994784.1"
                     /translation="MKSKALCRLRWFISVTSAGSFVCLSCVGPPPEVAPVENPGLDPQ
                     VRQYWEIKAAASTDEFTAYEATPDSLKPDWVRRFWKSKDPTPATPENEFLTEHGRRVD
                     YALQHYGHQGKGRPWDDRGEMYIRYGEPDEIEMSDEGFYDPGAPKSAIELRDNPIAAK
                     QAATRRGTGAETSIGGDPLFATSIERWYYSRYGLSFQFQPDLATGLVEAVPYVSTDRG
                     QVQTAREFVNSTEIRHEIYQHDYGGDFLPYTFSIARFRGPARNWEVDVNLALPLEKLA
                     LAGPDTNEVSCLRRMVIFDQAWHEVALDSTVIVKEVERYRRRGKLLVEQKTFQVPPGL
                     YTLSVEVRDLITRKVGIYKKPLLLPEYAAPDVQEVSDVVLASYVKPAEPSSLAFLRNG
                     WVVMPQPSRVFFPDDPLAFYFEVYNLRRGPDGKTHVKVGYEVVDFKSKKKIVTPLP"
BASE COUNT         1028 a         1415 c         1494 g          985 t
ORIGIN      
        1 atgagggctc gcagtgacat ggtgatgcgg tcgaagcaaa tctctctggc aacggtgagc
       61 ctgctcgtca cggtggcggg gtggcttgca cttgactgtg cgcggaagat tcagaatccg
      121 gtgggattgc cgcccggtct cggtgtgccg ggggaaacca cctatgtcca gattgcaccc
      181 gcctggactt ccgccggggg agtggcgttc agccacccgc acgatgtgat catcggacaa
      241 gaccttttca tctacgtggc ggatacggat aatgattgag tcgtcaaact cgacttcggg
      301 ggaaattttc tcgcttccta cccggtacca cacccgacgg cgataacaca ggatcggcaa
      361 ctcaacctgc ttgccgtcgg ccagcgcgac acgatttgct ccttccagcg aatatcggga
      421 accgaaacca ctcaggtgga cacggtcatc acccagcagg ggacgatctt caagcgattt
      481 tttaccagcg gaggagattt catccctgtt tatacggcac cggcgctgcc gccctccggc
      541 ggcttcagcg tctgtcagcc aaccattttt gccgggatag ccgcctcgcc ttttgccgac
      601 cgggagtatt tcatctccga cctcacccgc cgcaaggtga ttgcattcga ccgcttgaac
      661 cagccggata cgctgggaat tgccacggcg ggcggccgcg gcgtcaacag ccctttgggc
      721 ctggcggccc agccggtggg gcaaacctgg cggctgggga tggttgaagg aagggacgct
      781 caggtgttcc ggaatgagga taccgttttc acagccgtct acgcctgcag cacaggtcag
      841 gagttgatct gcgtcggcca gttccagttc gccaccgcga tcacttttga cgatttgggc
      901 aactggttcg tcgccgattc gggggccaat gaagtggcca agttcgaccg caacggaaac
      961 tttgtgctcc gcttcggccg caccggctcc ggcgacgggg aattcaacgg cccggccggc
     1021 attgccttct acgaccgcgt ggtctacgtg gcggattccg ggaacagtcg gatcgtgcgg
     1081 tatgttttga atacggacat tcgaccttga cttacccgtc agctcccgat gcgcattcat
     1141 tcaggtcggc tttgcggggc ttgcgatgac tcaatggccg gtttatatat agcacgtagg
     1201 cgtaaaagcg atgcgtgtct cagggtgggg ttgctcaaaa cacagaggag gtgactaccg
     1261 atgaaaaaag ttcttcttgc ggttttcctg atggccttct gcctggcgtt cctggttggg
     1321 atcgggtaca gccgtacggc actgccggac gatggcaacc cgccccctcc gatatgggtt
     1381 tgttgcacaa cgtacccggg atgtgttggc ggcggcgggt gggaagtatg cagcatctgc
     1441 tgcgattatc gggtgtcggt gcccgagtgc aaatgtgttg caccgggggt ggcgagttgc
     1501 cggtgcatat ttcggtaacg gtaagtaatt aaccgctcaa ttcacggtca gtgagcagcg
     1561 gagggaaaaa aaggaaacgg gcaccccacc tgtgcatcgc tcgcttgttg gtggttttgg
     1621 gcggacgtat atttgaataa gtagtgcctg ccgaagtggt ggaactggga gacacgctgt
     1681 gttcagggcg cagtgcccgc aaggcgtgcg ggttcgaatc ccgccttcgg catagaattt
     1741 cttgccggtg attggggtca ggccggaggc ctgacctatg ggtcagagag ctcgtagttc
     1801 ccatagccga ggcttttagc ctcggctgac aggagaaagc catgagaaca aaaatgaaag
     1861 tcgtggcttt cgcggcagtc gcgtcatctc tactttttct atcccccata ttagctcaaa
     1921 ggccgctctc tcaaaaagag aaggaactgt actacggcat caaggccgtc gccgcgccgg
     1981 aagagttcaa acaatttcaa aaactctccg gagacaagcg ggcggagtgg ctgcgcattt
     2041 tctgggtttc ccgggacccc acccccacca cgccggagaa cgagttttac aacgagcacc
     2101 aaaggcgggt ggcctacgcg gtcgagcatt tccacaccaa ccggctgggc tacctgtggg
     2161 acgaccgggg ggaggtttac atccgcttcg gcgagccgga cgagcggaat ttcaccctgg
     2221 agcggcatta cgaccgccag cagcagtggc attcggccta cgacctgcgg gacgtgctga
     2281 acaacccctt gaaattgcgg atgctcatgg cgcgtcaaaa ctttgacccc gaaggcggct
     2341 ttggcggcca gaacaccggc gccgattcag gagggtacgg cccccccagt gcggcggaca
     2401 tcaagaccga attagagaat gccggcaaag acttcgacac cttttccggc gaggtctggc
     2461 gctactaccg ctacggtttc accttccagt tccaggatga ccgcaacctg ggctactacg
     2521 aactggtgcc ctacgtggac gaatcgggcc gggggcagga tttccaggag ttcgtgcgcc
     2581 aggcggtggt ttcgaccgag atccaaaaag aaatttaccg gcacgactac ggcggccagg
     2641 cgatggacta tgccttcacg gtcacccgct tcagaggaga caacaaggtc aactacgtcg
     2701 ttgacgtgaa cctggggctg ccgttggata ggttgggcgt ggccggagag tctgcctcag
     2761 gcggacaggt ggagtgtatg cggcggatcg tgctgtacga tgaaaactac gagcccgtgg
     2821 cgagcgacag cacgacgctg gtggcgccag tcagtgccga aaaccgcaaa gggcagttgt
     2881 tgatcgagca aaagcggtat gaggtgaagc cgggcaagta caccctggcg gtggaagtcc
     2941 gcgacctggt cacgcagcgg atcgggattt acaagaagga acttttattg cccgagtata
     3001 tcgcgcccga cgtgcaggag atttcggacg tcgaattggc cagctacatc cgcccggcga
     3061 gcgattggga caaacggtac cggcacaagg gactggtggt gatgccccag ccctcgcggg
     3121 tcttctttcc cgaccaggtg atgcagtact actttgaagt gtacaacctg aagaaagcca
     3181 aagacggcaa ggcccgtttt tccatccggg ccgatgtcta cggctaccgc acccgcaagg
     3241 tggaatacac gttcaatccg cgcgactttg aggccgacac caccgacatt taccagaccg
     3301 gcaagatcaa cctgcgcgat ctggcccccg gccagtacat cctctcgctc aaggtgaaag
     3361 attacacgac cggcaaggag aagaccgccc tggcggggtt caagatcgcc agaagcgagt
     3421 gatctcagaa tttttgttcc tgccatagcc gaggttttca gcctcggatt ccattcattc
     3481 aggctggaag cctgacctat ggacggggtt gtagccctga ccaatttcaa aactcccgct
     3541 cctgcgccag agtcgaattc tccgtatact accagtggaa tgaaatcaaa agcactctgc
     3601 cgtcttcggt ggttcatctc ggtcaccagc gccggaagct ttgtttgtct ctcctgtgtg
     3661 gggccgccgc cggaagttgc cccggtagag aatcctggtc tggatcccca ggtgcgccag
     3721 tactgggaga tcaaagcggc ggcttccacg gacgaattta ccgcctacga ggcgacgccg
     3781 gacagtctca agcccgactg ggtgcggcgt ttctggaaat cgaaagaccc cactcccgcc
     3841 acgccggaga atgaattttt gaccgagcac ggccgccggg tcgactacgc cctgcagcac
     3901 tacggccacc agggaaaggg ccgcccctgg gacgaccggg gagagatgta cattcgctac
     3961 ggagagccgg acgaaatcga aatgagcgac gaaggatttt acgaccccgg cgcgccgaag
     4021 tcggccattg aactgcgcga caatcccatc gccgccaagc aggcggccac gcggcgcggc
     4081 accggcgcag aaacctcaat tggcggggac ccacttttcg ccacctccat cgagcgctgg
     4141 tactactccc gttacgggtt gtcgtttcag ttccagcccg acctcgccac cgggctggtc
     4201 gaggcagtgc cgtacgtttc caccgaccgg ggccaggtgc agaccgcgcg cgagttcgtc
     4261 aactccaccg agatccggca cgaaatctac cagcacgact acggcggcga cttcctcccg
     4321 tacaccttca gcatcgcccg cttccgcggt ccggcacgaa actgggaagt cgacgtcaac
     4381 ttagcgctcc ctctggagaa actcgcttta gccgggccgg ataccaacga ggtgtcctgt
     4441 ctcaggcgga tggtgatttt cgatcaggcc tggcacgaag tggctttgga cagtacggta
     4501 atcgtcaagg aagtggagcg ctaccggcgg agagggaagc ttttggtgga gcagaaaact
     4561 tttcaggtgc cgcccgggct ctacactctt tcggtggagg tgcgcgacct catcacccgg
     4621 aaggtgggca tctacaaaaa acctctgctt ttgccggaat acgccgcacc cgacgtgcag
     4681 gaagtatccg acgtggtcct agcctcctac gtcaaacccg ccgagccttc cagtctggcg
     4741 ttcttgcgca acgggtgggt ggtgatgccg cagccgtcgc gggttttctt tccggacgat
     4801 cccttggcgt tctacttcga agtgtacaat ctccgtcggg ggccggacgg caaaacccac
     4861 gtcaaagtgg gctacgaagt ggtggatttc aaaagcaaaa agaaaatcgt gacgcctttg
     4921 cc
//
LOCUS       DBNGZA010000478         2058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1050099, whole genome shotgun sequence.
ACCESSION   DBNGZA010000478 DBNGZA010000000
VERSION     DBNGZA010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1050099"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="AC16FU_10240"
     CDS             complement(<1..149)
                     /locus_tag="AC16FU_10240"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR2994785.1"
                     /translation="MANEPKKERRGLVVIHTGDGKGKTTAALGMAVRAAGYKMKVFIL
                     QFVKGT"
     gene            complement(149..>2058)
                     /locus_tag="AC16FU_10245"
     CDS             complement(149..>2058)
                     /locus_tag="AC16FU_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR2994786.1"
                     /translation="PLPDKRATVLAHFANLLPVGDAVLELLPLGPSALEIIDGHALEL
                     VGREKYGVPRDSEMLLLVEFDGGDLKSLVQRAKSILQKYPLSVPSVGEDDPVKQARLW
                     NVRKAVLPTLYRYDPVKRPVGFIEDSCLPSEQVPRFLRFLDGQFKRHNLQAGVYGHIG
                     DGNVHIRPLLNLRDPQDFEKMRALYEDVYAEVLRLGGSLAAEHGDGRLRAPRLKELYG
                     KEIYDLFVKIKKTFDPQNIFNPGVKFAENEFTQNINLAKLDHHCAACGKCNTVCPSYD
                     ITRTEILGSRGWVRALAVDHLSNAEARLAIDACLNCKACMVVCPAGVDTSEIVRKRRE
                     KDRSPWKRLALGTLTHPSLLRTGATFVGRTQIFWKFFLGSRNYPKFTGRAWSRRSNEG
                     ELDRAPVAYFPGCPDTFIANGVADAVLNVMDNLGVRTIVPKTFCCGAPLETIGYPDLI
                     KKNARKNLKLLEKFDAIVTSNGSCYWALKAFRDCFAPGEPEHAMAQKFTERVFDFSEY
                     IEKAAPQTFASGQRRMRVTYHDPCHLRAAGITLEPRALLRRLPGVEFVEMPDAERCCG
                     AAGAYRYLHYHEAMQIVGRKVRGIEAVKPDLVATGNSLCAFHIADGIHRKIPVLHVAQ
                     VVEKFLAEKGA"
BASE COUNT          427 a          628 c          570 g          433 t
ORIGIN      
        1 gtgcctttga cgaactgcag gatgaagact ttcattttat accctgccgc gcgcaccgcc
       61 atccccaagg ccgcggtagt tttccccttc ccgtcgccgg tgtggatgac gactaaaccg
      121 cgccgctctt ttttcggttc gttcgccatt aagccccctt ctccgccaag aatttttcca
      181 ccacctgcgc cacgtggagc accggaattt tcctgtgaat gccgtcggcg atatggaagg
      241 cgcagagcga attgccggtg gccaccagat cgggtttgac cgcctcaatc ccccgcacct
      301 tacgtccgac gatctgcatc gcctcgtggt agtgcaggta gcggtaggcc ccggcggcgc
      361 cgcagcagcg ctccgcatcc ggcatttcga cgaactcaac tcccggcaac cgccgcaaaa
      421 gcgcccgcgg ctcgagcgtg atccccgccg cccgcaggtg gcaggggtcg tggtaggtca
      481 cccgcattcg tctttgtcca gacgcaaatg tttgtggcgc agccttctcg atatattccg
      541 aaaaatcaaa aactcgctcc gtgaactttt gtgccattgc gtgctccggc tcgccgggag
      601 cgaagcaatc ccgaaaggcc ttcagtgccc agtagcagga gccgttgctg gtgactatcg
      661 catcgaattt ctccaagagc ttgagattct tccgtgcgtt tttctttatc aaatcggggt
      721 agccaatcgt ctccagcggc gcaccgcagc agaaagtttt cggtacgatc gttcgcacac
      781 ccaggttgtc catgacgtta agcacggcat cggccacccc gttggcaatg aaagtgtcgg
      841 gacagccggg gaagtacgcc acaggcgcgc ggtcaagttc tccttcatta gaacgccgtg
      901 accaggcccg gcccgtgaac tttggatagt tccgcgaccc taaaaaaaac ttccagaaga
      961 tttgcgtccg cccgacaaag gttgcgccgg tgcgcaggag cgagggatgc gtcaaagtgc
     1021 ctaaggccag gcgcttccaa ggcgaccggt ccttttcccg ccttttgcgt acgatttccg
     1081 acgtatccac gcccgccggg cagaccacca tgcaggcttt gcagttcagg caggcgtcaa
     1141 tagctaaacg cgcttctgca ttggagagat gatcaacggc cagcgcccgc acccagccgc
     1201 gggaacccaa gatttcagtc cgagtaatgt cgtaactggg gcagaccgtg ttgcacttcc
     1261 cgcaggccgc gcagtggtgg tccagtttgg ccaggttgat gttctgcgta aactcattct
     1321 ctgcaaattt gactccggga ttgaaaatgt tctgcgggtc gaaagtcttt ttgattttca
     1381 cgaagagatc ataaatctcc ttgccgtaga gctcttttag ccgcggcgcg cggagacgtc
     1441 catccccgtg ctcagccgcg agcgacccgc ccaggcgcag cacttcggca taaacatctt
     1501 catataacgc ccgcattttc tcgaagtcct gcgggtcgcg gagattaaga aggggccgga
     1561 tatggacgtt cccgtccccg atatggccgt acacccccgc ctggaggtta tgccgcttga
     1621 actggccgtc caggaagcgc agaaaacgcg gaacttgctc agacggcagg caggaatctt
     1681 cgataaaccc caccggccgc ttgaccggat cgtagcggta cagggtcgga agcaccgctt
     1741 tgcgtacgtt ccaaaggcgc gcctgcttga ccggatcgtc ttctccgact gatgggacag
     1801 atagagggta tttctgaagg atgctttttg cccgttgaac gaggcttttg agatctccgc
     1861 catcgaactc aaccaaaagg agcatttcag aatcgcgcgg aacgccatac ttctcccgcc
     1921 caactaactc aagcgcgtgg ccgtcgatga tttccaatgc cgacggaccc agcggcaaaa
     1981 gttccaaaac cgcgtcgccg acggggagca ggttggcgaa atgagccagc acggtcgccc
     2041 gtttgtcagg aagaggca
//
LOCUS       DBNGZA010000479         1932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2430511, whole genome shotgun sequence.
ACCESSION   DBNGZA010000479 DBNGZA010000000
VERSION     DBNGZA010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2430511"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="AC16FU_10250"
     CDS             <1..1082
                     /locus_tag="AC16FU_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994787.1"
                     /translation="ATLGSNTFMVDKFGIITSDDAWLDAMKRKNLTLEDVEAIREHCP
                     DCQYVEAQAWAQGRVKYGNQFMSDVDIVGLTHDFNEVTSINVDQHAAGHFLTEPDEER
                     RHQVCILGYELQDRLFGRINPLGRRVKVRGQEYTVVGLAKKRGSFLGWNQDNFVAIPI
                     STFGKYLGNDFWGRQSYTFYIKAADYRAMERAQDQVRLILRARRHVPYEKPDNFAIYT
                     ASTIMNVYRGFTTGAYVVMIGIASIALIVGGIVIMNIMLVSVTERTREVGVRKALGAR
                     KKNILLQFLIEAVTVALVGGMLGILLGVGLAQLLAVVSPLPAAVSWFWILIGLFVSTS
                     VGIFFGIYPAARAARLDPIEALRYE"
     gene            1232..>1932
                     /locus_tag="AC16FU_10255"
     CDS             1232..>1932
                     /locus_tag="AC16FU_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR2994788.1"
                     /translation="MRLNVFEIKEALGMAWDAIRMNKLRSFLTILGVFIGVTSVIGMA
                     SIIKGLDRSMARQIESLGSNVIYVSKHKPGIVIGNRSEEERNRKGITYDDAVAILERC
                     PAVAAISPENHYYPFPSGNRVKYKNQSARRPDFLGVLPAYEQVNNSFVSEGRFITESD
                     HQYRSLVTVIGQDVADALFGRLDPIGKEILVNNDKFLVIGVVEKRESILGQSQNNFVL
                     IPYGTFAKIHPEEKEL"
BASE COUNT          402 a          546 c          595 g          389 t
ORIGIN      
        1 tcgccactct cggttccaac accttcatgg tcgataagtt cggcatcatc accagcgacg
       61 acgcctggtt ggacgcgatg aagcgtaaaa acctcactct ggaagatgtg gaggcgatcc
      121 gggagcactg tccggactgc cagtacgtgg aggcgcaggc gtgggcgcag ggccgggtca
      181 agtacggcaa tcagttcatg tcggatgtgg acatcgtcgg cctgacccac gatttcaacg
      241 aagtgacctc gatcaacgtc gaccagcacg cggccgggca cttcctcacc gaaccggacg
      301 aggaacggcg ccaccaggtc tgcattctgg gttacgagct gcaggatcgg ctcttcggca
      361 ggatcaatcc tctgggccgg cgggtgaaag tccgcggcca ggagtacacg gtggtggggc
      421 tggccaagaa gcgcggctcg tttctgggat ggaaccagga caacttcgtg gccattccca
      481 tctccacgtt cgggaaatac ctgggcaacg atttctgggg ccggcagtcg tacaccttct
      541 acatcaaagc ggccgactac cgggccatgg agcgggcgca ggatcaggtg cggctcattc
      601 tgcgcgcccg acggcacgtg ccctacgaaa agccggacaa cttcgccatc tacaccgcct
      661 ccacgattat gaacgtctac cgcggtttca ccaccggcgc ctacgtggtg atgatcggga
      721 tcgcttccat tgcgctcatt gtcggcggca tcgtcatcat gaacatcatg ctcgtctcgg
      781 tgaccgagcg cacccgggag gtcggcgtgc gcaaagccct gggcgcccga aagaaaaata
      841 tcctcctgca atttctgatc gaagcggtca cggtggcttt agtcggcgga atgctgggga
      901 ttctcttggg cgtggggctg gcgcagcttt tggccgtggt ttccccgctg ccggcggcgg
      961 tttcctggtt ctggattttg atcgggcttt tcgtttcgac ctcggtcggc attttcttcg
     1021 gcatctaccc ggcggcgcgg gcggcccggc tcgacccgat cgaagcgctc cgctatgagt
     1081 gagaaaagcg ggtggaaggt ggtaggtcgt agggggtagg ggaaaaaggc ggtttgagga
     1141 gttaggattt gggaattagg ggaaagcaaa aacgaaatgt gagaatttcg gcaggcgagg
     1201 gttcatgcaa cctcgacacg gtttttaact tatgcgcctg aacgtttttg aaatcaaaga
     1261 agctctgggg atggcctggg atgccatccg gatgaacaag ctgcgctcgt ttctgaccat
     1321 cctgggcgtc ttcatcggcg tcacctcggt gatcggcatg gcgtcgatca tcaaagggct
     1381 ggatcggagc atggcccggc agatcgagtc cttaggttcc aacgtcatct acgtctccaa
     1441 acacaagccg ggaatcgtca tcggcaaccg atccgaagaa gagcgcaacc ggaaagggat
     1501 cacctacgac gacgcggtgg ccattctgga gcgctgtccg gcggtggcgg cgatctcgcc
     1561 ggaaaatcac tactacccgt ttccctccgg caaccgggtc aagtacaaaa accagagcgc
     1621 ccggcgcccc gactttttgg gcgttttgcc ggcgtacgag caggtgaaca attcgtttgt
     1681 ctccgagggg cgcttcatta ccgagagtga ccaccagtac cggtcgctcg tcaccgtcat
     1741 cggccaggac gtggccgacg ccctcttcgg ccggctcgac cccatcggca aagagattct
     1801 ggtcaacaac gacaagtttc tggtgatcgg ggtagtggag aaacgcgaaa gcatcctggg
     1861 ccagagtcag aacaacttcg ttttaatccc ctacggcacc ttcgccaaaa ttcatccgga
     1921 ggaaaaagaa ct
//
LOCUS       DBNGZA010000480         3173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_944131, whole genome shotgun sequence.
ACCESSION   DBNGZA010000480 DBNGZA010000000
VERSION     DBNGZA010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_944131"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /gene="asnS"
                     /locus_tag="AC16FU_10260"
     CDS             complement(<1..1175)
                     /gene="asnS"
                     /locus_tag="AC16FU_10260"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR2994789.1"
                     /translation="MKMPTCYIEEVGRYAGQEVTLRGWLYNKRSSGKIHFLLLRDGTG
                     IMQGVVTKDGSQNGVFEMSDQLTQESSVVVTGLVREDKRAPGGYELAIRNLEVIQIAR
                     DYPITPKEHGTGFLMENRHLWLRSSRQTAILKVRNEIIWAIREYFYQRKFILIDTPIL
                     TGAIGEGASTLFETQYFDLGKAYLAQTGQLYLEAACMALGKVYCFGPTFRAEKSKTRR
                     HLTEFWMVEAEVAYNDNQANMDLEEDLVVEVVGRVLERCSEDLKFLERDTAALEKVAK
                     HFPRVSYDEAVKILQKEGSQIQWGGDLGGEDETLFTQKYDQPVFIYNYPKSCKAFYMK
                     ENPADPRTVLCADCLAPEGYGEIIGGSQREDDYDKLLYRIREEKLPEETFGWYLDLR"
     gene            complement(1183..2040)
                     /locus_tag="AC16FU_10265"
     CDS             complement(1183..2040)
                     /locus_tag="AC16FU_10265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR2994790.1"
                     /translation="MAVLGVLALLGLAGALALFAVWHLVYRRVAAPPAPVFVCHRVSS
                     RFDLSISRQSSQNFLRICQTLAFLGYRGVTLSEALSQSPDAHIFALTFDDGYECFLRE
                     AYPVLQKFGWPATLFVVTGFVGQTSEWDLRWGWPRPRHLTWEQIEELHESGIEIGSHG
                     VTHCDLTRLSSKKLLFELQTSKKMLEDRLRTKIKSFAYPFGRYDVNVIEAVRAAGYSC
                     GVTGFPHPGEKRFDRYAVKRIGLTLFDTPLSLRMKVVRNRLTWVEEMKGLIVSRLAGG
                     TPLVKRRRV"
     gene            complement(2098..>3173)
                     /locus_tag="AC16FU_10270"
     CDS             complement(2098..>3173)
                     /locus_tag="AC16FU_10270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR2994791.1"
                     /translation="IADALESVGGSINAFTSREQTCYYAKVLDEHLPVAVEVLADILK
                     NSLLAPADLEKEKKVISEEIKDVQDSPSDLIHDLFAESIWEGHQLGRPIMGSVQSVTR
                     LARRDLVAYLRSHYVAPAVVVAACGNVSHRKVVRLVEREFDFSANGKGNGRVRTRPRA
                     KNRREVKPRETSQTHVCLGVPALPFRDEARHALLILNTILGGGMSSRLFQSVREKHGL
                     AYTIYSFQEFYQDTGLLGIYLGTDNRQVAQAIELVLRELRKFRREKLSKSELAAAKSQ
                     LKGSLMLGLESTSNRMNRLARHELFLNEYVSLDDTIRAINKVKSRDVTLLAQRLLDEE
                     SLAAAVLGPNHDSLIEKIDWSLS"
BASE COUNT          669 a          918 c          875 g          711 t
ORIGIN      
        1 ttgcgcaaat ccagatacca gccgaacgtc tcttccggta acttttcctc ccggatacgg
       61 tacaacagtt tgtcgtaatc atcctccctc tgggagccgc cgatgatttc gccgtaccct
      121 tcgggagcca ggcagtcggc gcagaggacg gttcgggggt cggctggatt ttctttcatg
      181 tagaacgcct tgcacgattt ggggtagttg taaatgaaga cgggttgatc gtacttttgg
      241 gtgaatagcg tttcatcctc gccgcccagg tcacctcccc attgaatctg gctgccctct
      301 ttctgcagga ttttgacggc ttcatcgtac gacacccggg gaaaatgttt cgctactttt
      361 tccaaagccg cggtgtcgcg ctctaagaac tttaagtcct cggaacagcg ttccagcacc
      421 ctccccacca cttcgaccac taaatcttct tccagatcca tgttggcttg gttgtcgttg
      481 tacgccactt cggcttccac catccaaaac tcggtcagat gccgccgggt cttcgacttc
      541 tcggcccgga aggtggggcc gaaacagtaa actttcccca acgccatgca ggccgcctcc
      601 aagtacaact gtccggtctg tgcgaggtag gctttgccca agtcaaagta ctgcgtttcg
      661 aataacgtcg aagcgccctc gccgatggcc ccggtcaaaa tgggcgtgtc aattaagatg
      721 aacttgcgct ggtagaagta ctcgcgaatg gcccagatga tctcgttgcg cactttcaaa
      781 atcgccgtct gtcgggaaga acggagccag aggtggcggt tttccatcag aaagccggtg
      841 ccgtgctctt tgggcgtgat cgggtaatcc cgggcaatct gaatcacctc gagattccgg
      901 atggccagct cgtagccgcc gggcgcccgt ttatcttccc gcaccagtcc ggtcaccacc
      961 accgacgatt cctgcgtcag ctggtcggac atttcaaaaa cgccgttctg cgaaccgtct
     1021 ttggtcacga ccccttgcat aatcccggtg ccgtcgcgca ggaggagaaa gtgaattttc
     1081 cccgacgagc gcttgttata cagccacccg cgcagggtca cttcctggcc cgcataccgt
     1141 cccacctcct cgatgtagca cgtcggcatc ttcattctac tcttacactc tcctccgctt
     1201 gaccaggggc gttcctccgg ccaaccggct gacgattaag cctttcattt cttcgaccca
     1261 ggtcaggcga tttcttacta ctttcatgcg caaagatagg ggcgtgtcaa aaagtgtcaa
     1321 cccaatccgc ttgacggcgt aacggtcaaa tctcttttcc ccggggtggg gaaagccggt
     1381 aaccccacac gagtaaccgg cggcccgaac cgcttcaatc acgttaacat cgtatcttcc
     1441 aaagggatag gcgaacgact taattttggt tcttaagcgg tcttccagca ttttttttga
     1501 ggtctgaagt tcaaacagta attttttgga agaaagccgg gttaagtcgc agtgtgttac
     1561 cccatgggag ccgatctcaa taccgctttc gtgcaactct tcaatttgtt cccaggtgag
     1621 atgacgcggt cgtggccagc cccagcgcaa atcccactca ctggtttgac ccacaaaacc
     1681 ggtgaccaca aagagggtcg ctggccaacc gaatttttgg agaaccggat aggcctcccg
     1741 gaggaaacat tcatacccgt cgtcgaacgt taaagcaaaa atatgtgcgt ccggagattg
     1801 agaaagggct tccgaaagcg ttacgccgcg gtaacccaga aaggcgagcg tttgacaaat
     1861 ccgcaaaaaa ttttgggacg attggcgcga gatcgaaaga tcaaaccggg acgagacgcg
     1921 gtggcagacg aaaaccggcg cgggcggtgc ggctacccgt cgataaacca aatgccagac
     1981 ggcaaaaaga gccagtgcgc cggctaatcc caggagggcg agaaccccaa gaacggccaa
     2041 ggcgatttct gccggctatt accgctcgta agagtcgttc atacgtaggg tcgagtctta
     2101 cgacagactc cagtcgattt tctcgatcag cgagtcgtga ttgggcccga ggaccgctgc
     2161 ggcgagactt tcctcgtcca gcaggcgctg ggccaaaagg gtcacgtcgc ggcttttcac
     2221 tttgttaatg gcacggatgg tatcgtctaa ggagacgtac tcgtttaaga agagttcgtg
     2281 ccgtgccagg cggttcatcc ggttggaggt ggactccagc ccgagcatca ggctcccttt
     2341 gagttgcgac ttggctgcgg ccagttcgct tttggaaagt ttttcccggc ggaacttgcg
     2401 gagttcgcgc agcaccagtt cgatggcttg agccacctgc cggttatcgg ttcccaggta
     2461 aatgcccagg aggccggtgt cctggtaaaa ctcctggaac gaataaatcg tataggctaa
     2521 accgtgcttt tccctcaccg actgaaagag gcgggaactc atgccgccgc cgagaatggt
     2581 gttcagaatc aaaagggcat ggcgcgcttc atcgcgaaag ggtaaagccg gcacgcccaa
     2641 gcagacgtgg gtttgcgacg tttcccgcgg tttgacctcg cgccggtttt tggcgcgcgg
     2701 ccgggtccgc acccgcccgt tccctttgcc gttggcggaa aaatcgaact cccgttccac
     2761 cagccggact accttccggt ggctgacgtt gccgcaggcg gccacgacca ccgccggggc
     2821 gacgtagtgc gaccgcaggt aggccaccaa gtcacgccgg gccagccggg tgacgctctg
     2881 gacgctcccc ataatcggtc gccccagctg gtgtccttcc cagatcgatt cggcgaagag
     2941 atcgtggatg agatcggagg gtgaatcctg gacgtcttta atctcttcgg aaatcacctt
     3001 cttctctttt tccagatccg ccggagccag aagcgaattt ttgagaatgt cggccaggac
     3061 ttcgacggcg accggcaggt gttcgtccag taccttggcg tagtagcagg tctgctcccg
     3121 cgaggtgaag gcgttgatgg agccgcccac cgattccagc gcgtcggcga ttt
//
LOCUS       DBNGZA010000481         4876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2109923, whole genome shotgun sequence.
ACCESSION   DBNGZA010000481 DBNGZA010000000
VERSION     DBNGZA010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2109923"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC16FU_10275"
     CDS             <1..251
                     /locus_tag="AC16FU_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994792.1"
                     /translation="WGDSTKFPLSYNDVWNNVAGNWHEISDQTGQAGNLSADPFFLDK
                     TDFHFQSASPLKDTGNPYYTDPDGSRSDLGIYGGPGAR"
     gene            complement(262..1524)
                     /locus_tag="AC16FU_10280"
     CDS             complement(262..1524)
                     /locus_tag="AC16FU_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HIR2994793.1"
                     /translation="MVSDAKRIFYDFHLHSHYSRATSKDMEAKTLALWAERKGITVVG
                     TGDFTHPKYVAELKEQFVPSGHGLFRLKNSQSPVHFLLTVEVNNIYHQGGRLRKIHTL
                     LFAPSFEVVDKINARLARRGDLAVDGRPTFTFSAKELVKIVLDLSAECFIVPAHAWTP
                     WFSVFGSNSGFDSLEECFGEETKHIHAIETGLSSDAAMNWRLSALDRVAVISNSDAHS
                     PAKLGREATVLSCNLDYQEIIDAIKTKDPKRFLYTIEFFPEEGKYHYDGHRDCRINWH
                     PNESKAHHNTCPVCGKGLTLGVLHRVEQLADRPDGYQPPGAIPQKHLIPLAEIIADAF
                     GQGVDTKAVQEEYLRLIRLLGSEFEILLDRTGEEIARVATPEVTEGIRRVRAGKVSIV
                     PGHDGVYGKISLFAEGEKIETQPQLSLL"
     gene            1637..2446
                     /locus_tag="AC16FU_10285"
     CDS             1637..2446
                     /locus_tag="AC16FU_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994794.1"
                     /translation="MPVYGYFGLGVIVLAEILLFAGLKLVGLFFTPVAWSGYILLVDA
                     LVFRAQSSSWLTTRRKEFLLLLPISIILWLIFEGYNQFIDNWHYVGLPENFFARVFGY
                     AWSFATIWPAILETNEFLLVNGVFAKTRVRPFLFSRPALNLLLVVGPAFLAIPILFPS
                     RWWAVLVWTGFVFLFDPLNFRRGLPSLITELRQGRPQLLLTLMFSGLICGFLWEFWNY
                     WAAAKWFYTVPLWGKVKLFEMPVVGYLGFPAFALEVFVMYHFVRGFLLRKF"
     gene            complement(2460..3812)
                     /locus_tag="AC16FU_10290"
     CDS             complement(2460..3812)
                     /locus_tag="AC16FU_10290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR2994795.1"
                     /translation="MLGQELIFEQLRKIIKQSPADQTEAVFSGSTVGTTRLVNSAIAE
                     SRSKESSEVYLRVVIGKQIGVVACDEFEENTLTSALKEALTVAKLHKEHPRPVVLPGA
                     QSYPTLNIYSTETASYPQKQCDQQVATLLQKTKQSHLALSGFFSTAQGEIAVLNSNGL
                     SAYQPFTSASTTLTLGQDGHVGTVNDLARDVGQINLAALWNQAYERAQIPDKTRTIEP
                     GNYTVVFEPRAVAHLLEWVNYIGFRSAAYQEKISFLSERLGKTVFGENISIYDDAGDT
                     SSLAFPFDFEGIPKRKTPLIERGVAKSIVYDTTSAAQDGVKTTGHAIVTNVAYNGAMA
                     VNLFLEPGKISKSELLRSVEQGLIIRRFYSLNGLLDPRNALVLGTTREGCLWLEKGKV
                     RANVPDVRINASLFDIFSNVVALSRETERVPYWWDGIGCITVPTIVVKNFPIAGLAKS
                     "
     gene            complement(3828..4745)
                     /locus_tag="AC16FU_10295"
     CDS             complement(3828..4745)
                     /locus_tag="AC16FU_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR2994796.1"
                     /translation="MPDKVTSLSEVIRTHVPDGSAVVMCTGMEQNIPFAAGHEIIRQK
                     KRDLTLIGPISDILFDQLIGAGVAKKVMAAWVGNVISGSAYNFRRAVEEKIPYPIEVV
                     DYSNFTVTLALHAGALGVPFLPTFSTLGSDVQKNNPHLKEFIEPFGQEKMIAVQALEP
                     EVAIIHVQRADPEGNCHLWGGLGITLDAARASKKVIVTAEEIVAPEVIASDPNRTLIP
                     GFLVTAVAEVPFGAHPSPVQGYYNRDHQTYVDYHEQTKSRTGFKKWLDKWAFGIQDQK
                     DYCNKLGDVRLKSLEVKTHAYAAAVDYGY"
BASE COUNT          951 a         1314 c         1367 g         1244 t
ORIGIN      
        1 tctggggcga ctcgactaaa ttccctctct cgtacaacga tgtctggaac aacgtcgccg
       61 ggaactggca cgaaatttcc gaccagaccg ggcaggcggg taacctttca gccgacccct
      121 tttttctgga taagaccgat tttcactttc aatcagcatc gccgctaaag gataccggga
      181 atccttacta caccgacccg gatgggtcgc gaagcgatct gggaatttac ggcgggccgg
      241 gagcgcggta gaactcgtct tttagagcag actcagctgc ggctgggttt cgattttttc
      301 accttcagcg aatagggaaa ttttcccgta gacgccgtcg tggcccggca ctattgagac
      361 tttccctgcc cggacccgtc tgattccttc ggtcacttcc ggtgtggcga ctctggcgat
      421 ttcctctccc gttcggtcga ggagaatttc aaactcgctc cccagcaggc gaattaaccg
      481 caggtactcc tcctgcaccg ctttggtatc gaccccctgg ccgaaggcat cggcaatgat
      541 ttcggccaat gggatcaaat gtttttgtgg aatggcccct ggaggctggt acccatccgg
      601 acggtcggct aactgctcca cccgatggag cacacccaga gtcaacccct ttccgcacac
      661 cgggcaggta ttgtggtgcg ccttgctttc atttgggtgc cagttgatcc ggcagtcgcg
      721 gtggccgtcg tagtggtact tcccttcctc ggggaaaaac tcgatggtgt agaggaagcg
      781 tttcgggtcc ttcgttttga tggcgtcaat gatttcttgg tagtcgagat tgcaggagag
      841 tacggtggct tcccggccaa gtttggcggg cgagtgcgca tcggagttgg aaatgacagc
      901 cacccggtcg agcgccgaga gccgccagtt cattgccgcg tctgacgaga gcccggtctc
      961 aatggcgtga atgtgcttgg tttcttcgcc gaaacattct tccaacgaat cgaagccgga
     1021 attggagccg aagaccgaaa accagggcgt ccaggcatgg gccgggacga tgaaacattc
     1081 tgctgacaga tcgaggacga ttttcactaa ttctttggcc gagaaggtaa aggtcggccg
     1141 gccatcgacc gccagatcac cccgccgggc taaccgggcg ttgattttgt ccacgacctc
     1201 aaacgaaggg gcaaagagga gagtgtgaat tttccggagc cgacctcctt ggtggtaaat
     1261 gttattcacc tctaccgtga gcaggaagtg caccggcgat tggctgtttt ttaagcgaaa
     1321 tagcccatgg ccggagggga cgaactgttc ctttagttcc gctacgtact ttggatgggt
     1381 gaaatcgccg gtgccgacaa cggtgatgcc tttccgttcc gcccagagcg ccagcgtttt
     1441 ggcttccata tccttgctcg tggcacggct gtagtgggag tgcaggtgga aatcgtaaaa
     1501 tatacgcttt gcatcggaca ccatcggcga aatatattgc ctgaaatgta tggtgcaatc
     1561 aatcgcgact gctgaccagc gccaagtaag tttccgtcag gagatatccg ggagtcagct
     1621 tcccattcgc cagcgtatgc cggtctacgg ctacttcggg ctgggtgtta tcgtcctggc
     1681 tgagattttg ctgtttgcgg gcctgaaact ggtcggcctc tttttcacac ccgttgcttg
     1741 gagcgggtac atcctgttgg ttgatgcgct ggtgttcaga gcgcagagca gctcctggct
     1801 gactacccgg cgtaaagaat tcctgttgtt gctccccatc tcgataattt tgtggctcat
     1861 ttttgaagga tacaaccaat tcatcgacaa ctggcactat gtgggcttgc ccgaaaattt
     1921 ctttgcgcgt gtgttcggct acgcctggtc tttcgccacc atctggccgg cgattctgga
     1981 aaccaatgaa ttccttttgg tcaatggagt ttttgcgaaa acgcgcgtcc ggccttttct
     2041 cttctcgcgt ccggcgctaa atttacttct ggttgtcggt cccgcctttc tcgctatacc
     2101 cattctgttt ccctcgcgtt ggtgggccgt tctggtctgg accggtttcg tcttcctctt
     2161 cgatccgctc aacttccgcc gcggcttgcc ctctctcatc acggagctgc gacagggcag
     2221 gcctcagttg cttttgactt tgatgttttc gggattgatc tgcggttttc tctgggaatt
     2281 ctggaactat tgggccgcgg cgaagtggtt ctacacggtg cccctctggg gcaaggtgaa
     2341 actcttcgaa atgccggtgg tggggtatct gggttttccg gcctttgccc tagaggtgtt
     2401 cgttatgtac cacttcgtgc gggggttcct gctcagaaaa ttctaatttt tagaagaaat
     2461 taggatttgg ccagaccggc gatggggaag tttttcacca cgatggtcgg tacggtgatg
     2521 cagccgatcc catcccacca gtagggtacc cgctcggttt cccgcgaaag ggcaaccacg
     2581 ttggagaaga tgtcaaacaa actcgcgttg attcgcacgt ccggcacgtt cgcccttact
     2641 ttcccctttt ccagccacag gcagccttcc cgggtcgttc caagcacgag cgcgttgcgc
     2701 ggatcgagca agccgttgag tgaatagaaa cgccggataa tcaggccttg ctctacggaa
     2761 cgtaaaagtt cgcttttgga gattttgccc ggttccagga aaaggttgac cgccatggcg
     2821 ccgttgtagg ccacgttggt gacgatggcg tggccggtgg ttttgacgcc gtcctgtgcc
     2881 gcgctagtgg tgtcgtagac gatgctcttg gccacccccc gctcgatgag cggcgttttc
     2941 cgcttgggaa ttccctcaaa atcgaatgga aaagccagcg acgaggtatc acctgcgtca
     3001 tcgtagatag aaatgttttc tccgaaaacc gtctttccta aacgctccga aaggaagctt
     3061 attttctcct gatacgccgc cgagcggaag ccgatgtagt tcacccactc cagaaggtgc
     3121 gctacggcgc gcggctcgaa caccaccgtg taatttccgg gttcaatcgt tctggtcttg
     3181 tccgggattt gagcgcgctc gtatgcttga ttccataggg cagcgaggtt aatctgcccg
     3241 acgtctcgcg ctaggtcatt taccgtgccg acgtgaccat cttggcccaa cgtaagggta
     3301 gtcgaggccg aggtaaacgg ctggtaggcg gaaaggccgt tggagttgag caccgcaatt
     3361 tcgccttggg cggtggagaa aaagccggac agggcaaggt gtgactgctt ggttttttgc
     3421 aaaagggtag ccacttgctg gtcgcactgt ttttgcggat aggaggcggt ctcggtagag
     3481 taaatgttca gagtcggata cgattgcgct ccgggcagca cgacgggccg ggggtgttcc
     3541 ttgtggagtt tggctacggt cagcgcctct tttagggcgg aggtcagggt gttttcttca
     3601 aattcatcgc aggctaccac cccgatttgc ttgcctatca ctacccgcag gtagacttca
     3661 cttgactctt tggagcggct ctccgcgatg gccgagttca caaggcgggt ggtgcccacc
     3721 gtactgccgc tgaaaaccgc ctcggtctgg tcagccggcg attgcttaat aatttttctc
     3781 agctgttcaa aaatcagttc ttgaccgagc atagggattc atttctctca gtaaccgtaa
     3841 tccactgcgg cagcgtaggc gtgggttttg acctcaagcg atttcagccg cacatctccc
     3901 agtttattac aatagtcttt ttgatcttgg atgccaaacg cccatttgtc cagccatttt
     3961 ttaaaacccg tacgcgattt tgtctgttca tggtaatcga cataggtctg gtggtcgcgg
     4021 ttgtagtagc cctgcaccgg tgacgggtgc gctccgaacg gcacttccgc cactgcggtg
     4081 acaaggaatc cgggaatcaa cgtacggttt gggtcgctgg cgatgacttc cggggctaca
     4141 atttcttcgg cggtaacgat cactttctta ctcgcccgcg ctgcatcgag cgtgattcca
     4201 agcccgcccc agagatggca gttgccttcc ggatcggccc gctggacgtg gatgatcgcc
     4261 acctccggct ccagcgcctg caccgcaatc atcttctcct gaccgaatgg ctcgatgaat
     4321 tctttcaagt gcggattatt tttttgcacg tccgagccga gcgtcgaaaa cgtgggcagg
     4381 aaggggacgc ccagtgctcc ggcgtggagc gccagtgtca ccgtgaaatt ggagtaatcg
     4441 accacttcaa ttgggtacgg gattttctct tccaccgccc ggcgaaagtt gtaggccgag
     4501 ccggaaatta cattccccac ccaggcggcc atgacttttt tcgccacacc cgctccgatt
     4561 aactggtcaa acagaatgtc cgaaattggg ccgatcaagg tcaggtcgcg ttttttctgg
     4621 cggataattt cgtgcccggc ggcgaaggga atgttctgct ccataccggt gcacatgacg
     4681 accgcagagc cgtcggggac gtgggttcta attacttcgg atagagaggt tactttatca
     4741 ggcatcagga aatcattttt ttcaggcggg actaagagtc ccacctacgc aactttgtcg
     4801 gccacaaaca aaaatgcccc tcgcggggca ttcgctatta cttcttcatt ggtttcttca
     4861 tcatttcttt catcat
//
LOCUS       DBNGZA010000482         7924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_415820, whole genome shotgun sequence.
ACCESSION   DBNGZA010000482 DBNGZA010000000
VERSION     DBNGZA010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_415820"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="AC16FU_10300"
     CDS             <1..610
                     /locus_tag="AC16FU_10300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR2994797.1"
                     /translation="ILEHEKKVERFLGLAERGRTPRSAELPELKLPPQPLPAEADLDL
                     LLATTLQQREALNQIYDKFVVRRDRLDHTPSILPVPGRLTRGFGLHSDPFTGQSEHHA
                     GVDLAAPWGTPVQASATGRIVYAGWRSGLGKAIIINHGRGFETYYGHLSAIRVTPGQR
                     VTKGQLIGAVGSTGYSTGPHLHYEVHLRTMPQNPMRYVLIER"
     gene            840..1499
                     /locus_tag="AC16FU_10305"
     CDS             840..1499
                     /locus_tag="AC16FU_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2994798.1"
                     /translation="MKKATAKLDPAERLKEEPETTLHGAPGGADGFKEDPAVLALIER
                     VKEKDDRAFQELVERYRNQVAGLAYKMVGDYDDAKDISQIVFVKTYYNLQRFDTTKKF
                     STWLYRITINASIDYWRKYHKHRHEPLEEVINRPDESQAGPEVQYQRKKTREEISRAL
                     VALNDKQRQVFVLRDMEGLDIAEVAKIMNMPQVTVRWYLHRARTKLRTQLVKKRRRMG
                     G"
     gene            1503..1970
                     /locus_tag="AC16FU_10310"
     CDS             1503..1970
                     /locus_tag="AC16FU_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR2994799.1"
                     /translation="MIDCRICNKLLNRYDFQSDVWAKEMEAHCRACPPCQQEFLLYQS
                     VDAELKQAFREEPPARLWEGFWAELARKLRGRSWREKVGDQLESVWLWLNRPVVGFVP
                     AYALAALLVLALSVGLITRSGRPGFQSDLVVHPQQSVSAEVVDGVTVYQIASR"
     gene            1976..3055
                     /locus_tag="AC16FU_10315"
     CDS             1976..3055
                     /locus_tag="AC16FU_10315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2994800.1"
                     /translation="MNLPVLRRRRGRVVLWGTLLVFPLALSGCSSKAELSQIENSVAS
                     VMDKVGPDVVFISATLPPAKEKLQPTVRRGCGVILKDGRILTTSSLLAGAKECTIRFQ
                     DGSQVAPEGIARICADFETNVGVIVLKGASRKNADFAVAKNVKPGVIGIALGNTPYSK
                     GLAVAVGAIGVSWVGGVDAYDTNLLTFDAPHLGSYSGIPIFNSKGELVGLIEGRFADT
                     ESPWLVVPATTALAIAERLEKDSCIERGFLGVFSHTNCQEEYGGYDRVVDPKKPAGVY
                     LSGVVPGSPAAKAGFREGDLIFSINGRPVNSCVALRKLVTAVKAGDDVVVEYRHQGKV
                     FKRTIAVDNLPPCCLSSRRCSDRPT"
     gene            complement(3129..5315)
                     /locus_tag="AC16FU_10320"
     CDS             complement(3129..5315)
                     /locus_tag="AC16FU_10320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR2994801.1"
                     /translation="MQKLLDDLNPPQREAVTHPSGPLLILAGAGSGKTRVLTYRIAHL
                     IAERGVPAEQVLAVTFTNKAADEMRARIRTILGVEKIPLVMSTFHSFCARLLRREAEL
                     LGFPRTFSIYDDDDQVGLLKHIIEDLQLQNRQLSAAGAQNQISLAKNRLQSPADFAAD
                     ARPGSGGTKGLVAQIFAEYQKRLQQQGAMDFDDLLVKVVELFRRHPERLAYYQNHFAQ
                     ILVDEYQDTNHAQYLLVKFLAEKHRNLAVVGDDDQSIYRWRGADLRNILEFERDFPDA
                     RVVKLEQNYRSPQVILDAAWQVVSRNTGRKEKKLWTDREGGEKISLTLTFDDRMESRW
                     VAEEIARRRAALDLSFSDFVVLFRTNAQSRLLEDALRDRGFPYVIVGGVRFYQRREVK
                     DVIAYLRVLANPRDDTACLRILNVPRRGIGAATEKKLIDLAREKNLPLFELLATPELW
                     PRSSPKIKKELQKLSAFLTDLKPRVAALPVAELIETIVMRTGLVEELQVEDAQAQESR
                     AENVKELIAAAAEFADRTENPTVDTFLEEVTLLSDIDRWDDAQERITLMTLHQAKGLE
                     FRVVFLAGMEEGLFPLSRSLADPDELEEERRLFYVGCTRAKEKLYLSACRHRRRFGEM
                     TNLKSRFLDELPPEHLETTDLGFSTTISWEAAQRSARQVELIPEFDAVTARVKVGSRV
                     IHPTWGQGKVLQREGSGENLRLLVQFTSGGPKKLLAKYANLEIAQY"
     gene            5443..6828
                     /locus_tag="AC16FU_10325"
     CDS             5443..6828
                     /locus_tag="AC16FU_10325"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HIR2994802.1"
                     /translation="MPTAAIIDNVLAAIGDTPLVRLKKVTPPGGAEILAKAEFLNPGG
                     SVKDRMALYIIEQAERSGLLTPGGTIVENTSGNTGVGVAMVAALKGYRAIFTMPDKMS
                     TEKINFLKAFGAKVIVTPTNVPADSPLSYYETAKRIARETPNSFYLNQYHNPLNIEAH
                     YKITGPEVWEQTQGKLDCCVAGIGTGGTLSGVGRFLKEKNKNIKVIAVDPVGSVFHHY
                     FKTGKLPTPHVYKVEGIGEDMLTKAMDFAVVDEIYQATDKESFLMARRLTTEEGLFVG
                     GSSGAAVSVAVKVAEKLGPGKRLVVILPDSGDRYLSKIYSDEWMKDNGFLEAEPRLGT
                     VRNILHLRKQKVIMARSGDRVAQVIAKMKKYDISQLPVVDDGKVLGMITEVDLLNYLV
                     AGKHRVREAIKAIIERDVEIVDPETSISTLSEIFSAGERNAVLVMEKGKLVGLVTKID
                     MIDYLAKKFKE"
     gene            6876..7178
                     /locus_tag="AC16FU_10330"
     CDS             6876..7178
                     /locus_tag="AC16FU_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR2994803.1"
                     /translation="MSFVKVAKLDELIPGQAKTVTLNGREIALFNVEGEIFATDNTCL
                     HMGGPLGEGDLASAVVTCPWHGWEYNVKTGEALTAPGEYIQKFEVRVQNGDIEVAV"
     gene            7347..7508
                     /locus_tag="AC16FU_10335"
     CDS             7347..7508
                     /locus_tag="AC16FU_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994804.1"
                     /translation="MERGKTAEQVVVTFRPDTISGQALADKIADVGVPGFFRRQVRRL
                     GHFLHRETK"
     gene            7569..>7924
                     /locus_tag="AC16FU_10340"
     CDS             7569..>7924
                     /locus_tag="AC16FU_10340"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR2994805.1"
                     /translation="MAPVTTGKKAPTFQLKGIDGKPYEFKGDGAPALTVLSFFKNSCP
                     TCQLTVPYLEKLYQAYQQKGVRFWGIEQDPADQSKEFARQYGLTFPVLPDVLPYPVSN
                     SYGVSIVPTIYLIDGTG"
BASE COUNT         1682 a         2252 c         2356 g         1634 t
ORIGIN      
        1 cattctggag cacgagaaga aagtggaacg cttcttaggc ttggccgaac gcggccgtac
       61 gccgcgctcc gcggaattgc cggagctcaa gctgccgccc caacccttgc ccgcggaggc
      121 cgatctcgac ctgctcctgg ccaccaccct ccagcaacgc gaagcgctga atcaaattta
      181 cgacaagttt gtggtccgcc gcgaccggct ggaccacacc ccctccatcc tgcccgtgcc
      241 ggggcgtctg acccggggat ttggtttgca cagcgatccc ttcaccggcc agtcggaaca
      301 ccacgccgga gtcgatctgg ccgctccctg gggtactccg gtgcaagcgt ccgccaccgg
      361 gcggatcgtc tacgccggct ggcgctcggg actggggaag gcgattatca ttaaccacgg
      421 ccgcggtttt gagacctact acggtcacct gtcggcgatc cgggtgacgc ccggacaaag
      481 ggtcaccaaa gggcagttga tcggcgcggt cggttccacc ggctactcca ccggcccgca
      541 cctgcactac gaagtccacc tgcgcaccat gccgcagaac ccgatgcgct acgtccttat
      601 cgaacgttaa ataattctac ccagacgcag gaaataatga aaccccgtca tctgggacgg
      661 ggtttcatta tttggagcgc ggtcggacga ccactctcct cccgagagcg gggaacaaaa
      721 atggcgtgcg ctccgtaaaa aagggtgtat attctcctcg gtgtggggaa gtgagaaacc
      781 caccagtgat agggagtgga atctccaccc aacagagtct tttccccggc gtattaaaga
      841 tgaaaaaggc aaccgccaaa cttgacccgg ccgagcgcct gaaagaggag ccggagacaa
      901 ctctccatgg tgcaccaggt ggagccgatg ggttcaaaga agacccggcg gttctggcct
      961 tgattgaacg ggtcaaggag aaggacgacc gggccttcca ggagttggtg gaacgctacc
     1021 gcaatcaggt ggcgggtctg gcttacaaga tggtgggaga ttacgacgac gccaaggaca
     1081 tctcccagat cgtgttcgtc aagacctact acaacctgca gcggttcgat accaccaaga
     1141 aattttccac ctggctgtac cggattacga tcaacgcctc catcgactac tggcgcaagt
     1201 accacaagca ccgccacgaa ccgctggagg aagtgatcaa ccggcccgac gaatcacagg
     1261 cggggccgga ggtgcagtac cagcggaaga aaacgcggga ggagatcagt cgggcgctgg
     1321 tggctttgaa cgataagcag cgccaagtct ttgtgcttcg ggatatggaa ggattggaca
     1381 ttgccgaagt cgccaagatc atgaacatgc cgcaagtgac ggtgcggtgg tacctgcacc
     1441 gggcgcggac caagttgcgg acacagttag tcaaaaagcg gaggcggatg ggaggatgaa
     1501 gcgtgatcga ttgtcgtatt tgtaacaagc tcctcaaccg gtacgatttc cagagcgatg
     1561 tgtgggcgaa agagatggaa gcccactgcc gggcgtgtcc gccctgccag caggaatttc
     1621 tcctctacca gtcggtggat gccgaactca agcaggcgtt ccgcgaagag ccgccagcca
     1681 gattgtggga agggttttgg gccgaattag cgcgcaaact ccggggccgc agctggcgag
     1741 agaaagtggg tgaccagttg gagagcgtgt ggctctggtt gaaccggccg gtggtggggt
     1801 ttgtcccggc ctatgccctg gctgccctgc tggtcctggc tctctccgtg ggtttgatta
     1861 cccgcagcgg ccgacccggg ttccagagtg acctcgttgt ccacccccag caatcggttt
     1921 ccgccgaagt ggtggacggc gttaccgttt accagatcgc ttcccgttaa gacgtatgaa
     1981 tcttcccgtt ctgcggcgcc ggagggggag ggtggttctg tggggtacgc ttcttgtctt
     2041 tccgctggcg ctctccggct gttccagcaa agccgagctt tcccagatag aaaattccgt
     2101 tgcgtcggtg atggacaagg ttggccccga cgttgtgttc atctccgcca ccctgccgcc
     2161 ggctaaagaa aaactccagc cgaccgtgcg ccggggttgc ggcgtgatcc tcaaagacgg
     2221 ccggattctc actacctcca gcctcctggc cggagccaag gagtgtacca tccgcttcca
     2281 ggatggaagc caagttgctc cggaaggtat agcgcgaatc tgtgccgatt ttgaaaccaa
     2341 tgtcggcgtg atcgtcctca aaggtgcgtc ccgaaaaaat gccgattttg ccgtggcgaa
     2401 gaacgtcaaa cccggtgtca tcggaatcgc cctggggaat accccttatt ccaagggttt
     2461 ggccgtggcg gtgggggcga tcggcgtttc gtgggtgggc ggcgtggatg cctacgacac
     2521 gaaccttttg acttttgatg ctccgcacct cggcagttac tccggcattc cgattttcaa
     2581 cagcaagggg gagttagtcg gtttgattga aggccggttc gccgataccg aaagcccctg
     2641 gctggtcgtt ccggcgacca cggccttagc catcgccgag cggctcgaga aagattcctg
     2701 cattgagcgc gggtttctgg gcgttttctc gcacaccaac tgccaggaag aatacggggg
     2761 atacgaccgg gtggttgatc ccaagaaacc ggcgggagtg tacctctccg gcgtcgtgcc
     2821 gggaagccct gccgccaagg ccggctttcg ggagggggac ctcatcttct ccatcaacgg
     2881 ccggccggtg aattcctgcg tggccctgcg caaactggtg accgcggtga aagcaggtga
     2941 cgacgtcgtc gttgaatacc ggcaccaggg gaaagtcttc aaacggacga ttgcggtgga
     3001 taatctcccc ccgtgctgtc tttcgtcacg ccgctgttcg gatcgaccca cctaaagaag
     3061 agcgatccga gaatagggcg actatttccc gtttctaaaa actgattcct aaatattctg
     3121 cgagtccctc agtattgggc gatttctaaa ttggcgtatt tggccagaag tttcttcggc
     3181 ccgccggaag tgaactgcac caacagtcga agattctcac ctgaaccttc ccgctggagt
     3241 actttgccct gtccccaggt ggggtgaatt acccgggaac cgaccttgac acgggcggtg
     3301 accgcatcga attccgggat cagttccacc tgccgcgccg agcgttgagc cgcttcccag
     3361 gagatggtgg tggagaaacc gaggtcggtc gtttccaaat gctccggggg tagttcatcc
     3421 agaaagcgcg atttgaggtt ggtcatttcg ccgaaacggc ggcggtggcg gcaggcggag
     3481 aggtagagtt tctctttcgc ccgggtgcag ccgacgtaaa ataaccgccg ttcctcttcc
     3541 agttcatccg gatcagccag cgatcgggag agcgggaaaa gtccctcctc cattccggcc
     3601 aggaatacca cacggaactc cagccctttg gcctgatgca acgtcatgag ggttatgcgt
     3661 tcctgggcgt cgtcccagcg gtcgatgtcc gataataacg tcacctcttc taagaaagta
     3721 tctaccgtcg gattttccgt tcggtcggca aattcggcgg cggcggcaat cagttccttc
     3781 acgttttccg cccggctttc ctgggcctgc gcatcttcga cctgcaactc ttccaccaag
     3841 ccggtgcgca tcacgatggt ctcgataagc tcggccaccg ggagcgctgc caccctgggc
     3901 ttcaggtcgg tgagaaacgc cgagagtttc tgcagttctt ttttgatctt gggtgaggag
     3961 cggggccaca gttcaggcgt agcgagaagt tcgaacaggg gcaggttctt ttcgcgggcc
     4021 aaatcgatta acttcttttc ggtcgccgct ccgatgccgc gccggggcac gttcagaatc
     4081 cgcaggcagg cggtatcgtc ccggggattg gcgagcaccc gcaggtaggc aatcacgtcc
     4141 tttacctcgc gcctctggta aaagcgcact cctccgacaa tgacgtaggg aaaaccccga
     4201 tcccgcagcg catcttccaa caggcgcgac tgggcgttgg tccggaagag cacaacaaaa
     4261 tcggaaaatg acaggtctag cgctgcccgc ctccgggcaa tttcttccgc cacccagcgg
     4321 gattccatcc gatcgtcaaa ggtcaacgtc aggctgattt tctcgccgcc ttcgcggtca
     4381 gtccacaatt tcttttcttt ccttcccgtg ttgcgcgaca ccacctgcca ggcggcatcc
     4441 aggatcacct gcggggagcg gtagttttgc tccaatttca ccacccgggc gtcggggaag
     4501 tcgcgctcga actccaggat gttccgcagg tcggcgcccc gccagcggta gatcgactgg
     4561 tcgtcgtccc ccaccaccgc cagattgcgg tgcttttcgg ccagaaattt caccaagagg
     4621 tactgggcgt ggttggtatc ctgatactcg tccaccagga tttgggcaaa gtggttctgg
     4681 taataagcga ggcgctcggg gtggcgccga aacagttcaa caaccttgac gagcaggtca
     4741 tcgaaatcca tggctccctg ttgttgtaaa cgtttctggt attccgcaaa tatctgggct
     4801 accagcccct tggtgcctcc cgaacccggg cgcgcatcgg cggcgaagtc ggcgggactc
     4861 tggagccggt ttttcgccag gctgatctgg ttctgcgctc ccgcggcgga caattgccgg
     4921 ttttgcagct ggagatcttc gataatatgc ttgagtaagc ccacttgatc atcatcatcg
     4981 tagatggaaa aagtgcgcgg gaagcccaaa agttccgctt cgcggcgcag gagccgggcg
     5041 cagaaagaat ggaacgtgct catcaccaaa ggaattttct ccactcccaa gatggtgcga
     5101 atgcgcgcgc gcatttcgtc ggcggctttg ttggtgaagg tcaccgccag aacctgttcc
     5161 gccggcaccc cgcgctcggc gatcaagtgt gcaatccggt aggtcaggac gcgggtctta
     5221 cccgaaccgg cgccggccag aatcaaaagc ggtccggacg gatgggtgac cgcctcccgc
     5281 tgaggcgggt tcaaatcatc caatagtttt tgcaactttt ctccttactt tttcctttga
     5341 acactatcgc ccttggcctg atggccactg cattttacgc aaccgcgtca ttgacagcaa
     5401 ggccaaaaag gtttttgtta cggctccgag agtgtgcagg cgatgccgac cgcagccatt
     5461 atcgacaatg tcttagccgc tatcggcgat actccactgg tgcggctgaa gaaagtcacc
     5521 cccccggggg gcgccgaaat tctggctaaa gccgagttcc tcaatcccgg cggttcggtc
     5581 aaagaccgga tggccctcta catcatcgaa caggccgagc ggagcggtct tttaacgccc
     5641 ggagggacga ttgtggaaaa tacctccggc aacaccggtg tcggcgtggc gatggtggcg
     5701 gccctcaaag ggtaccgggc tatcttcacc atgccggaca agatgtcgac cgaaaagata
     5761 aatttcctca aggccttcgg cgccaaagtg atcgtcaccc ccaccaatgt cccggccgat
     5821 tcgcctcttt cgtactacga aacggccaag cggattgccc gcgaaacgcc caattcgttc
     5881 tatctcaatc aataccacaa tcccctgaat atcgaagcgc actacaaaat aaccggaccg
     5941 gaagtctggg agcaaaccca ggggaaactc gactgctgcg tggccgggat cggcaccggc
     6001 gggacgctct ccggggtggg gcgcttcctc aaggaaaaga acaaaaacat caaggttatc
     6061 gcggtggacc cggtcggttc ggttttccat cactacttca agacgggcaa actgcccacg
     6121 ccgcacgtct acaaggtcga agggatcggc gaagatatgc tgaccaaggc gatggacttt
     6181 gcggtggtgg atgagattta ccaggcgacc gacaaagagt cgtttttgat ggcgcggcgg
     6241 ctcaccaccg aagagggact tttcgtgggc ggttcctccg gtgcggcagt gtcagtggcg
     6301 gtcaaggtgg cggagaaact cggtccgggc aagcggctgg tggtgatcct gcccgattcg
     6361 ggtgaccgct acctctccaa gatttactcg gatgaatgga tgaaggataa cggcttcttg
     6421 gaagccgaac cgcgcctggg cacggtgcgc aatatcctcc acctgcgcaa gcagaaagtc
     6481 atcatggccc gttccggcga ccgggtggct caggtgatcg ccaaaatgaa aaagtacgac
     6541 atctcgcaat tgccggtggt ggatgacggg aaggtgctgg ggatgatcac cgaagtcgat
     6601 ttgctcaact acctggtggc gggcaagcac cgggtgcggg aggccatcaa agcgatcatt
     6661 gagcgcgacg tggagattgt cgatcccgag acttccattt caaccctgtc ggagatcttt
     6721 tccgccggcg aacgcaatgc cgttctggtt atggaaaaag ggaaactggt cggcctggtg
     6781 accaaaattg atatgatcga ttacctggcc aagaagttca aggagtgatc tccaggtcgt
     6841 tgagatacga atttggtact cctccaagtt caagtatgag ttttgtcaag gtcgccaaac
     6901 ttgacgaact gattcccggg caggccaaga ccgtgaccct caacggccgg gagatcgcac
     6961 tcttcaacgt ggagggcgaa atcttcgcca ccgataacac ctgtctccac atgggcgggc
     7021 cattagggga aggagatctg gccagtgcgg tggtcacctg cccctggcac ggttgggagt
     7081 acaatgtgaa aaccggcgag gcgttgaccg ctcccgggga gtacatccag aagtttgaag
     7141 tgcgggtgca gaatggcgac attgaggttg ccgtctgagt ttgtcacgtg aaaggtttcc
     7201 ccggaaaggt catggtcacc gcctcaaaag agacgccttg gcccggcggt ctcgtccagg
     7261 cgactctgga ggtggagggc tttacctgcg ggctctgagc cagccacacg cgccaggttc
     7321 tggccgcgct accgggggtg tcaaaggtgg agcggggaaa gaccgcggag caagtggtcg
     7381 tgaccttccg gcccgatacg atttcgggac aagctctcgc cgataagatt gccgacgtgg
     7441 gagtgcccgg ctttttcaga cgacaggtac ggcgattggg gcacttcctc catcgagaaa
     7501 caaagtagtt gtggacttgc agacggtatt tatcttgcgt atgatagaga aaacgaggag
     7561 gaacgtatat ggctcctgta actaccggga agaaagcacc gacctttcaa ctcaaaggaa
     7621 ttgatggaaa gccgtacgaa ttcaaaggcg acggcgcccc cgcactcact gtcctctcgt
     7681 ttttcaaaaa cagctgcccc acctgtcaac ttaccgtacc ctacctggaa aaactatacc
     7741 aggcttatca gcaaaagggg gttcgcttct gggggattga gcaagacccg gcggaccagt
     7801 ccaaagaatt tgcccgccag tacggtctga catttccggt actgcccgat gtcctgcctt
     7861 atccggtgtc aaattcttac ggtgtttcga ttgtgccaac tatttatttg attgacggca
     7921 ccgg
//
LOCUS       DBNGZA010000483         2861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2324971, whole genome shotgun sequence.
ACCESSION   DBNGZA010000483 DBNGZA010000000
VERSION     DBNGZA010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2324971"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /gene="rsfS"
                     /locus_tag="AC16FU_10345"
     CDS             <1..434
                     /gene="rsfS"
                     /locus_tag="AC16FU_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR2994806.1"
                     /translation="GRHTAAARLPAAPKHRLTIARPSRSRASSGRLALLAGQLALEKK
                     ARQVLVLDLRGLSAVCDFFVLCSADAHVHAQAVADNIVLRLEAKGQKVWHKEGYPDTS
                     WILLDYVDFVVHIFLDEARSFYNLERLWGDAPSRILDENHR"
     gene            851..2272
                     /gene="rho"
                     /locus_tag="AC16FU_10350"
     CDS             851..2272
                     /gene="rho"
                     /locus_tag="AC16FU_10350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR2994807.1"
                     /translation="MKTVANGPAGRRNKVNADVAVAEPPEPQLAEVVTPTPGEEATTP
                     VIQVGEPPLSEKPELSITELKNKTIAQLLKTAEDLEIPGCSGLRKQELIFKILEAKTQ
                     KSGLIFAEGVLELMEEGYGFLRSPDYNYLPGPDDIYVSPSQIKRFDLRTGDTVSGQVR
                     PPKEGERYFALLKIEAVNFENPEVAKHKTLFDNLTPLYAQKRFKLETEQPEIAMRIMD
                     LLTPTGKGNRGLITSPPKAGKTILLQKIANSITTNHPEVKLIVLLIDERPEEVTDMKR
                     SVKGEVISSTFDEPAERHVQVAEMVIEKAKRLVEHQNDVVILLDSITRLARAYNAVVP
                     HSGKILSGGVDSNALQKPKRFFGAARNIEEGGSLTVIATALIETGSRMDEVIFEEFKG
                     TGNMEIVLDRRLSDRRIFPAIDINRSGTRKEELLLTPEELNRVWIMRKFLNEMNAIEA
                     MEFLQDRMRKTKDNAKFLESMKG"
BASE COUNT          657 a          862 c          809 g          533 t
ORIGIN      
        1 ccggccggca taccgcggcg gcacgcctgc ccgcggcacc caaacaccgc ctgaccatcg
       61 cccgaccgtc ccgttcccgg gcctcttccg ggcgtttggc ccttttggcc ggccagctgg
      121 ctttagaaaa aaaggcgcgg caggtgctgg tgcttgattt acgcggcctc tccgcggtgt
      181 gtgatttctt cgttctttgc tcggccgatg cgcacgtgca tgcccaggcg gtggcggata
      241 acatcgtcct ccgcctggaa gcgaaagggc aaaaggtctg gcacaaagag gggtacccgg
      301 atacatcctg gattctctta gactacgtcg atttcgtcgt gcacattttc ctggacgaag
      361 cgcggtcgtt ctacaacctg gaacggttgt ggggggatgc gccgagccga atcttagacg
      421 agaatcaccg gtgatcctgt tggcgtccgg ggtatggacc tctgatagcg tagccaccca
      481 cagtccagcg agcggccgtg cggctcgcac atgatgcaac cggggcagaa aagatttccg
      541 gccggaaagc aaaaaaattc ttgacattct ttattctccc ttttatgttg cccaggcacc
      601 ggaaagaaag atgttgcctc aaacatagac gtgggtaggt gcgttataca ggcagaatcc
      661 tcttcggtac gggtggtacc aacctccgga agatgcaggg cagagaccta tacccttgca
      721 tgggccggaa accagagcat aaagtaatct cgtacgcagt cgataatccc agcagggctg
      781 attcacaaga caaccgcagt accgcgctac cgtgaacaac cgtaaacctt ctaccgctac
      841 aaaggaaacc atgaaaactg tcgctaatgg gccggccggc cgccggaaca aggtgaatgc
      901 cgacgtggcc gtcgccgagc cgcccgagcc ccaactcgct gaggtcgtaa ccccaacccc
      961 gggcgaagaa gcaaccaccc ccgtcattca agtgggggaa ccgccgcttt ccgaaaagcc
     1021 ggaactctcc atcaccgaac tcaaaaacaa gaccatcgcc caactcttga agaccgccga
     1081 ggacttagaa atccccggct gttcgggcct gcgcaagcag gaattgattt tcaaaatctt
     1141 agaggccaaa acgcagaaaa gcggcctcat ctttgccgag ggtgttctgg aacttatgga
     1201 agaagggtac ggcttcctgc gctcacccga ctacaactac ctgccggggc cggacgacat
     1261 ctacgtctct ccctcgcaga tcaagcgctt cgatctgcgc accggcgaca ccgtctccgg
     1321 ccaggtgcgg ccgcccaagg aaggcgagcg ctactttgcg cttttgaaaa tcgaggcggt
     1381 caactttgaa aatccggaag tggccaagca caaaacgctc ttcgacaacc tgacgcctct
     1441 gtacgcccag aaacggttca agctcgaaac cgagcagccc gaaatcgcga tgcgcatcat
     1501 ggaccttttg acccccacgg gcaagggaaa ccgggggctc atcacctcgc cgcccaaagc
     1561 ggggaagacc atcctcctgc agaaaatcgc caactcgatt accaccaacc atccggaagt
     1621 caaactgatc gtgctcttaa tcgacgagcg gcccgaagag gtgaccgaca tgaagcgctc
     1681 ggtgaagggc gaggtgattt cctccacctt cgacgagccg gcggagcggc acgtgcaggt
     1741 ggcggaaatg gtgatcgaga aggccaagcg gctggtggag caccagaacg acgtggtgat
     1801 tctccttgac tccatcaccc ggctggcccg ggcctacaat gcggtggtgc cgcactccgg
     1861 caagattctc tccggcgggg tcgattccaa cgcgctccag aagccgaagc gcttcttcgg
     1921 cgcggcccgg aacatcgaag agggcgggtc tttgaccgtc atcgccacgg ccctgatcga
     1981 aaccggcagc cgaatggacg aggtgatctt cgaggaattc aaaggcaccg ggaacatgga
     2041 gatcgtcctg gaccgaaggc tttccgaccg ccggattttc ccggccatcg acatcaaccg
     2101 ctccggcacg cgcaaagaag aactgctcct gacgccggaa gaactgaacc gggtctggat
     2161 catgcgcaaa ttcttaaacg agatgaacgc catcgaggcc atggagttcc tccaggaccg
     2221 gatgagaaag accaaagaca acgccaagtt tttggagtcg atgaaagggt aagcccggct
     2281 tcagcgtttt tcgcgcagtt cccccaaaag cagctggtgt aaatccttcg taggggcgga
     2341 cgtttacgtc cacccgaata tttgtagccg aggtttcccg gtacgggtcg tatctgacca
     2401 acgttggcgg tctatacgag tctcggactt cggtcaggca ggatgcctga cctatggaag
     2461 gaaggaattt ttcctcttcc atagccgagg cttccagccg cggaagacac tctagccctt
     2521 gcaacccagg gttgatcctc gtaactcgaa agaccggctc gtcccggagt caaagacaag
     2581 agtcgggctc agggacgagc tcggcacgag tcgtatcgac ccgactacca gttctttggg
     2641 ctaatccgcc tgtggcgtat tcgcccctac ctaccccctg gcacttcgtg cctgtccccc
     2701 ttgcaaaggg ggacagaccc ggagcggagc cgaagggtca gggggtcgat ggtaggggcc
     2761 agacaccgtg cctaccgagc agagccaagc atgtaatcac tcgcttgttt ttcttgccgc
     2821 tcagccagaa gcgtctggac gagttcctgc aattcgctca c
//
LOCUS       DBNGZA010000484         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_97332, whole genome shotgun sequence.
ACCESSION   DBNGZA010000484 DBNGZA010000000
VERSION     DBNGZA010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_97332"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="AC16FU_10355"
     CDS             <1..981
                     /locus_tag="AC16FU_10355"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR2994808.1"
                     /translation="DYDRSGTDAYGSDYRPGERVEGDPADYLDGLVRRVVPIERYPDR
                     CAVDLVTFHVLRPYYYSRPYPVYDPYAYDPFFDCGFVYIGYPVGSEVFIDGIFFGYAP
                     IYVPRVTVGFHVLYVRHSGFDGYSYRLNVRRNQVYRFKADSFKDWKPTRVMFKKSDRP
                     LFDEAIRKKIILPPRTDTKFKQAESASGGNRGRDGQRVEGKEKRDFQQSVPSREERVF
                     RSEMRSDWPGAEQQRIEVRKSERSGGEMRTQNSDSRQREGIQVKREGPQRDAQFRSSE
                     REKREVRQNDGRQRVEKQTRVKEVDRSERPVGGSRDEMKGEKEFHSKGKK"
     gene            1051..1452
                     /locus_tag="AC16FU_10360"
     CDS             1051..1452
                     /locus_tag="AC16FU_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994809.1"
                     /translation="MLKKLSVGLVAAVAFAFLWVSISSAGIVRRSNSGNEPKRASAAP
                     PPQYEPQSTQPPDAGWSGTDSYKQNTKEKERGKSDEPKKGQGEEARRTEKRKAYDSFI
                     DRNGNGIDDRYEKKTRGTEESGSKKKKKPGE"
     gene            1611..>1885
                     /locus_tag="AC16FU_10365"
     CDS             1611..>1885
                     /locus_tag="AC16FU_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR2994810.1"
                     /translation="MAKKALVLSGGGAKGAFQFAAEKYAREVKSYRWDVIAGVSVGAL
                     NGVMLAMQKYQRLEEIWQTISNDKVYTGGLNVWSVAKVLLFGARSFYG"
BASE COUNT          452 a          493 c          614 g          326 t
ORIGIN      
        1 gattacgacc ggtccggcac cgatgcatac ggatccgatt accgccccgg cgagcgggtg
       61 gagggcgatc cggccgatta cctggacgga ctcgtccgcc gcgtggtgcc gattgagcgc
      121 taccccgacc gctgtgccgt cgatttggtg acgttccacg tcctgcggcc ttactactac
      181 tcccggccct acccggttta cgacccgtat gcgtacgacc cgttctttga ctgcgggttc
      241 gtctacatcg gttacccggt gggttcggag gtcttcatcg acgggatttt cttcggctat
      301 gcgccgattt acgtcccgcg ggtgacggtg gggtttcacg ttctctacgt gcggcactcc
      361 ggcttcgacg gctactccta ccggctcaac gtgcggcgga atcaggtcta ccgcttcaag
      421 gccgacagtt tcaaagactg gaaaccgacc agggtgatgt tcaagaaatc cgacaggccg
      481 ttgtttgacg aggccattcg gaagaagatt atcctcccgc cgcgcacgga tacaaagttc
      541 aaacaagcgg aatccgcctc gggcggaaac agggggcggg acggccagcg ggttgaaggc
      601 aaagagaaaa gagatttcca gcaaagcgta ccgtctcggg aggagcgtgt atttcggagt
      661 gagatgcggt cggactggcc cggcgctgag cagcagcgga tcgaagtccg caaatcggaa
      721 cgatccggcg gggaaatgcg cactcagaac tccgactcac gccagcgcga aggtatccag
      781 gtgaagcggg aagggccgca gcgcgatgcg caattccgtt cctccgaacg tgagaagcgg
      841 gaagtgcgtc aaaatgacgg ccgccagcgg gtagaaaaac agacgcgcgt caaggaagtc
      901 gaccggtcgg aacgacccgt tggcggcagc cgggacgaaa tgaaaggtga aaaagagttt
      961 cacagtaagg gcaaaaagta gttatactga ggttatgcaa tgcctatgtc gaagaaaaat
     1021 gaaaagaagt gcccacccaa gggaggcggc gtgctgaaga aactctcggt tggcctggtg
     1081 gcggcggttg cgttcgcctt cctgtgggtg agtatttcct cggcgggcat cgtgcgacgg
     1141 tcgaactccg gcaacgaacc caaacgcgcc agcgcagcgc cgcctccgca gtacgagccg
     1201 caatcgacgc aaccgccgga tgcgggatgg tccggtacgg attcgtacaa acaaaatacg
     1261 aaagagaaag agcgagggaa atctgacgag ccgaagaaag ggcagggcga agaagcccgc
     1321 agaaccgaga agcgcaaggc gtacgacagc ttcatcgacc gcaacggcaa cggcatcgac
     1381 gaccgctacg agaagaaaac ccggggcacc gaagagtccg gcagcaagaa gaaaaagaaa
     1441 ccgggagagt gagtcgcatt cgtattcggt ttcggagatg aaccgaacca ccgaggggta
     1501 gtcggggtcg tccccgcacc cgacagaggt ggctgaaaca ggatcagggc cagacggaag
     1561 tttggccctg attcattagc ggataactta tcaactcatt gggaggaagc atggcgaaga
     1621 aggctctcgt tttaagcggc ggaggtgcca agggcgcgtt ccaatttgcg gcggaaaagt
     1681 acgcccgcga ggtgaagagt taccggtggg acgtcattgc gggagtctcg gtgggtgcgc
     1741 tcaacggcgt gatgctggcg atgcagaaat accagcgtct cgaagaaata tggcagacga
     1801 tttccaacga taaagtttat accgggggat tgaacgtctg gtcggtcgca aaggtgctgc
     1861 tcttcggggc gaggtccttt tacgg
//
LOCUS       DBNGZA010000485         2134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_627719, whole genome shotgun sequence.
ACCESSION   DBNGZA010000485 DBNGZA010000000
VERSION     DBNGZA010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_627719"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16FU_10370"
     CDS             complement(<1..213)
                     /locus_tag="AC16FU_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994811.1"
                     /translation="MSGLPNTLRNALVLLTFFASGTLSAFAAVPNLINYQGKLTDGSG
                     NPITNPTQAVIISIYNVASGGSPLWAE"
     gene            complement(275..1729)
                     /locus_tag="AC16FU_10375"
     CDS             complement(275..1729)
                     /locus_tag="AC16FU_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994812.1"
                     /translation="MKRTLLFVVGLAAFLSQIAMTAVPNLINYQGKLTDGSGNPITNP
                     TQAVIISIYNVASGGSPLWAETLSVSVANGIYNVLLGSIHPIPDNIFADLRYLGVKVG
                     GDLEMTPRQKIASVPSALRVATVDGATGGSINGSLSFGNSATPMEYIYESGLTNPTRA
                     VLAHSPAFPNYGLAYDDLNEQMNFLGAGNPVLSIGLDSRFVGVNRSTQVTSAEYFGIS
                     APVATGDYGGMYISTGGNDGWPFYGYSTLSNTAWTYLNGQTGKWNLHLGGADRLTVTP
                     AGNVGVGTINPAARLHVVAGGVQAALCQGSFAGKFTGDVDVLGTLTKSSGAFKIDHPL
                     DPANKYLYHSCVESPDLDNIYDGIVVTDAKGNTTVTLPDWFGALNRDFRYHLTVIGQF
                     ARATVSKEIASNQFSIQTDKPNVKVSWQVTGVRQDAYANAHRIPVEEEKPVAERGKYR
                     HPKELGMPETAGINYEMRHKMEQTSEQKKAESQK"
     gene            complement(1748..>2134)
                     /locus_tag="AC16FU_10380"
     CDS             complement(1748..>2134)
                     /locus_tag="AC16FU_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994813.1"
                     /translation="GAVNKDFRYQLTAIGAPGPNLYIAQEVSGNQFKIAGGTPGMKVS
                     WQVTGIRQDAYAKAHRIPVEEEKPAAERGKYRHPKEQGMPETLGMNYEETQKMEVERK
                     QQEEQRAKMEQERLLMEQKTKANGNK"
BASE COUNT          413 a          567 c          619 g          535 t
ORIGIN      
        1 ttccgcccag aggggtgagc ccccggaagc aacgttgtag atggaaataa tgaccgcctg
       61 ggtggggtta gtgatggggt tacccgaacc gtcggtcagt ttgccctggt agttgattag
      121 gttcggcaca gcggcgaagg cgctcaaagt acctgaggcg aagaatgtga gtagaaccag
      181 tgcgttgcgc agtgtgtttg ggagacccga catgactcct ccttttctgg tgaatcggtg
      241 accgatgact ctaaaaggcc tgatcattct gcccttactt ttgagattct gccttttttt
      301 gttccgatgt ctgctccatt ttgtgtctca tttcgtagtt tatcccggca gtttccggca
      361 tacccagttc cttgggatgg cggtacttgc cgcgctcggc gaccggcttt tcttcttcca
      421 ccggaatgcg gtgggcgttg gcgtaagcat cctgccttac gccggtcacc tgccaggaaa
      481 ctttgacgtt cggcttatcg gtttggatgg agaattggtt gctggctatc tccttggaaa
      541 cggtggcccg ggcaaactgc ccgatgactg ttaaatggta acggaaatcg cgatttaagg
      601 ccccgaacca atcgggcaaa gttacagtgg tgttgccctt ggcatccgtg acgacaatgc
      661 cgtcgtagat gttgtccaga tccgggcttt ccacacagga gtggtagaga tatttattgg
      721 ccgggtctaa cggatggtcg attttgaacg caccggatga tttggtcaag gtaccgagaa
      781 catcaacgtc tccggtgaac ttcccggcaa aactcccctg acagagcgcc gcttgaacac
      841 caccggctac aacgtgaagg cgagccgcag gatttatggt gccaactcca acattcccgg
      901 caggagtgac cgtcagacgg tcggccccgc ccaagtgcag attccatttg ccggtctgac
      961 cgttcagata ggtccaggcc gtattgctca gggtgctgta cccgtaaaac ggccagccgt
     1021 cgttgccgcc ggtagaaatg tacattccgc cgtagtcacc ggtcgctacc ggggccgaaa
     1081 tgccgaaata ttctgcgctg gtaacctgtg tggaacggtt cacccctaca aaacgactgt
     1141 ccaaaccgat gctcaatacc ggattccctg cccccaggaa gttcatctgc tcgtttaggt
     1201 catcataggc aagcccgtag ttgggaaagg cgggcgaatg ggccagcacc gcccgggtgg
     1261 gattggtgag gcccgactcg tagatgtatt ccatcggcgt tgcagagttg ccgaagctca
     1321 gagagccgtt gatgctcccg ccggtcgctc catccaccgt cgccacccgc agggcggagg
     1381 ggacggaggc gatcttctgg cggggagtca tttcgaggtc gccgcccact ttgacgccga
     1441 ggtagcgcag gtcggcaaag atattgtccg ggatggggtg aatgcttccc aggagcacgt
     1501 tgtaaatgcc gttggccact gagacggaaa gggtttccgc ccagaggggt gagcccccgg
     1561 aagcaacgtt gtagatggaa ataatgaccg cctgggtggg gttagtgatg gggttacccg
     1621 aaccgtcggt cagtttgccc tggtagttga tcaggttcgg tacggctgtc attgcaattt
     1681 gagacaagaa ggccgccagc cctactacga acaacaaggt gcgtttcata cgctcctcac
     1741 tttcggttta cttattcccg ttcgccttgg ttttctgttc cataagcaag cgctcttgct
     1801 ccattttggc tcgttgttct tcttgttgct tgcgttctac ttccattttt tgggtctctt
     1861 catagttcat ccctaaggtt tccggcatac cctgttcctt ggggtgtcgg tatttgcccc
     1921 gctcggcggc cggtttttcc tcttctaccg gaatccggtg ggctttggca taggcatcct
     1981 gccggatgcc ggtaacctgc caggaaactt tcataccggg agttccgcca gcaattttga
     2041 actgattgcc ggaaacttcc tgggcaatgt ataggttggg ccccggagct ccgatggcgg
     2101 taagttggta gcggaaatcc ttgttcactg ctcc
//
LOCUS       DBNGZA010000486         1040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1791957, whole genome shotgun sequence.
ACCESSION   DBNGZA010000486 DBNGZA010000000
VERSION     DBNGZA010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1791957"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..>1040)
                     /locus_tag="AC16FU_10385"
     CDS             complement(191..>1040)
                     /locus_tag="AC16FU_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR2994814.1"
                     /translation="VLVDEQTASASEIVAGAIQDWDRGVIIGDTTFGKGLVQQVFPVS
                     GSRDLFLKLTTARWYVPSGRTIQKPERSTKHPELLGQEGDEEDTTQSKPQKPDKTEVY
                     RTKAGRLVYGGGGIIPDIIMDRERWRPIEWNLERQRLFFQFAVVYTTKHPDLPQNFGV
                     DEAMLAEFKSFIKEKKFDYKTPLEVELDSLASLAAQDNKQEVFADAVTLLRQKITAEK
                     EKDYDASRDYIAKAIKREILLKKFGEKGYYEELIFKDDPYVTRALDVLSNKSQYTRLL
                     RPLAER"
BASE COUNT          212 a          290 c          288 g          250 t
ORIGIN      
        1 atgtcccaca gggttgacct ctccccaacc ttctccttaa aggagatgga gcataaatct
       61 cagattccca ggaaagtacg gataaatttt accctcccct gtaggggagg gtagggtggg
      121 gtcaagaatg acccaaaagg gctgacctct cccccaaccc tctcctaaag gagagggagt
      181 agactgaaga ttaccgctcc gctaaaggcc gcaggagtcg ggtgtactga ctcttgttgg
      241 acaagacatc cagcgcccgg gtgacgtaag ggtcgtcttt gaagatcaat tcctcgtaat
      301 accccttctc gccgaacttc ttcaaaagga tttcacgttt aatcgctttg gcaatgtagt
      361 cgcggctggc gtcgtagtct ttttcttttt cggcggtgat cttctggcgg agcaaggtga
      421 ccgcatcggc gaagacctcc tgcttgttat cctgagcggc cagcgaagcc agactgtcga
      481 gttcgacttc taaaggcgtt ttgtagtcga actttttctc tttgatgaac gatttgaact
      541 cggctaacat ggcctcatcg actccgaaat tctgaggcag gtcggggtgt ttcgtagtgt
      601 aaaccaccgc gaactggaag aagagccttt gccgttccag gttccactca atcggccgcc
      661 agcgttcgcg gtccataatg atgtcgggga taatgccgcc gccgccgtag accagtcgtc
      721 ccgccttggt gcggtagact tcggtcttgt cgggtttctg gggtttggac tgggtggtgt
      781 cttcctcgtc accctcctgt cccaaaagtt ccggatgctt ggtgctccgc tccggttttt
      841 gaatcgtgcg gcccgagggg acgtaccagc gggcggtggt cagtttcaaa aaaaggtcgc
      901 gggagccgct caccggaaaa acctgctgca ccagcccctt gccgaaggtc gtatcgccga
      961 taatgacgcc ccggtcccag tcctgaatgg cgcccgccac aatctccgac gcgctggcgg
     1021 tctgctcatc gaccaaaacg
//
LOCUS       DBNGZA010000487         1464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1688236, whole genome shotgun sequence.
ACCESSION   DBNGZA010000487 DBNGZA010000000
VERSION     DBNGZA010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1688236"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16FU_10390"
     CDS             complement(<1..371)
                     /locus_tag="AC16FU_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994815.1"
                     /translation="MISIKKFAALRIFLTWFGAGGIFFSSLVETQDRLGGEVVASQAN
                     RKLPGGRGRGKGWIAPLFACFLLLPQLAGAYPFVDSLIVASKTFSFGSETVIEIFLYN
                     DSAVFGMSIPLVIKSQTGGAFG"
     gene            489..>1464
                     /gene="gmd"
                     /locus_tag="AC16FU_10395"
     CDS             489..>1464
                     /gene="gmd"
                     /locus_tag="AC16FU_10395"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR2994816.1"
                     /translation="MKRALITGITGQDGSYLAEFLLSKNYEVWGMVRRASTENFDRIN
                     HIRDQIILKQADLLDQLSIITILKECQPNEIYNLAAQSFVPTSWDQPVLTGEFNALGV
                     TKVLEAIRLVDKKIKFYQASSSEMFGKVREVPQTEKTPFYPRSPYGVAKVYGHFITVN
                     YRESYGIFATSGILFNHESPRRGLEFVTRKVSDGVARITLGLSKELALGNLDAKRDWG
                     FAGDYVKAMWLMLQQNEPRDYVIATGKNHSVRELVQVAFQHVGLDWEKYVVVDPKFVR
                     PAEVDVLLGDATLAKTKLGWQPEESFEGLVKMMVDHDVKRVLSGMQAQP"
BASE COUNT          351 a          395 c          401 g          317 t
ORIGIN      
        1 ccgaacgctc cgccagtttg cgacttgatt accagaggaa tagacatccc aaagacagct
       61 gagtcattgt ataaaaatat ctcgataacg gtttcacttc cgaaactgaa ggtcttggac
      121 gcgactatta aggaatctac aaatggatag gccccagcaa gctgcgggag gagcaaaaaa
      181 caggcaaata agggtgcgat ccaacctttc cccctccctc tgcctcctgg gagcttccgg
      241 ttggcttggc ttgcgaccac ttctcctccg agtcggtctt gagtctcaac gaggctcgag
      301 aagaatatac cacctgcgcc gaaccaggtc aaaaaaattc ttagtgcggc aaacttttta
      361 atggagatca aagactttgc ttgtcccttt gacaggtaac caaccccatc cccaatccca
      421 attccggcaa ttgacgtacc cccttccctg tagttaatta gcgtccctat gctgcagaga
      481 atcactcaat gaagcgggcg ctcatcaccg gcattaccgg ccaggatggc tcgtacttgg
      541 ccgaatttct gctttccaaa aactacgagg tctgggggat ggtgcgccgg gcgtcaaccg
      601 aaaattttga ccggattaac cacatccgcg accagatcat cctgaagcag gccgacctgc
      661 tcgaccaact ctcgatcatc acgattttga aggaatgtca gccgaacgag atctacaatt
      721 tggccgcgca gtcgttcgtc cccacttcct gggatcagcc ggtattgaca ggggaattca
      781 atgccctggg ggtgacgaag gtgctggaag cgatccggct ggtggacaaa aaaatcaagt
      841 tctaccaggc ctcatcttcg gagatgttcg gcaaggtgcg ggaagtgccg cagaccgaga
      901 aaaccccctt ttacccccgc tcgccttacg gcgtggccaa agtctacggg catttcatca
      961 ccgtcaacta ccgggagagt tacggcattt tcgccaccag cgggattctc ttcaaccacg
     1021 aatcgccccg gcgggggctg gagttcgtca cccgcaaagt ttccgacggg gtggctcgga
     1081 ttactttggg gctgtctaag gaactggcgc tcggcaactt agacgccaag cgcgactggg
     1141 gttttgccgg cgattacgtg aaagcaatgt ggctgatgct tcagcagaat gaacctcggg
     1201 actatgtgat cgccaccggc aagaaccatt cagtgcggga gttggttcag gtcgcctttc
     1261 agcacgtggg cctggactgg gagaagtacg tggtggtcga tccgaagttc gtccgcccgg
     1321 ccgaagtgga cgtattactg ggagatgcaa ctttggccaa aaccaagctg ggctggcagc
     1381 cggaggagag ttttgagggc ctggtgaaaa tgatggtgga tcacgacgtc aaaagagttc
     1441 tttcgggaat gcaggcgcag ccgc
//
LOCUS       DBNGZA010000488         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2431664, whole genome shotgun sequence.
ACCESSION   DBNGZA010000488 DBNGZA010000000
VERSION     DBNGZA010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2431664"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1570
                     /gene="dxs"
                     /locus_tag="AC16FU_10400"
     CDS             <1..>1570
                     /gene="dxs"
                     /locus_tag="AC16FU_10400"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR2994817.1"
                     /translation="HSPADLKRLSQKDLVSLAAQIREMILEVVSANGGHLATNLGSVE
                     LTLALHRVFDSPQDQIIWDVGNQAYAHKLVTGRYEKFATIRKQGGLSGFLRRDESPHD
                     QFGAGHASTGISAGTGFACGRDLAGGKQQVVVIVGDGALTGGLSFEGLNNAGALGKNL
                     LVVLNDNSMSISKNVGAIAKFLTTFLTDEHYNKLKTDIWELTGRSKQGRKIREIISHI
                     DENIKGFFVPGLLFERLGFRYFGPIDGHDIPQLIRVFTHLKTLPGPLLLHVLTVKGKG
                     YVPAEHDPAKFHGVGAFDKVTGKANAAGVTAYQAVFGETLVKIAEKNSKVAAITAAMC
                     TGTGLVEFAKKFPERFFDVGIAEEHGATFAAGLACQGIKPIYAIYSTFLQRAFDQVVH
                     DVALQNIPVLFAVDRAGLVGDDGPTHHGVFDLSYLRAIPNFTVAAPKDGNELKDLLWS
                     AIGWQDGPVAIRYPRGGIPDKLQDTVRPSPYGTWQVEREGEEVVIFAVGAMVYPALEA
                     VTRLLVDGLSPTVV"
BASE COUNT          313 a          473 c          474 g          310 t
ORIGIN      
        1 ttcactcgcc ggccgatctc aagagactct cccaaaagga tctggtatcc ctggcggctc
       61 agatccgcga aatgattctg gaagtggttt cagccaacgg cgggcatttg gctaccaact
      121 taggttcggt ggaactgacc ctggcattgc accgggtctt cgattcgccc caagaccaga
      181 tcatctggga cgtggggaac caggcctacg cgcacaaact ggtcaccggc cgctacgaaa
      241 agttcgcgac catccggaag caggggggac tctccggttt cctgcgccgg gatgagagcc
      301 cgcacgacca gttcggcgcg gggcacgcct ccaccggcat ctcggccggc acgggctttg
      361 cctgcggacg tgatctggcg ggcggcaaac aacaggtggt ggtcatcgtg ggggacggcg
      421 cgcttaccgg cggtctctcc ttcgaagggc tcaacaatgc cggcgctctg ggcaagaacc
      481 tcttagttgt gctcaacgac aactcgatgt cgatttcgaa aaacgtgggg gcgattgcca
      541 agttcctcac tacctttctc accgacgagc actacaacaa attgaaaacc gacatctggg
      601 aactgaccgg ccgctccaaa cagggtcgga agattcggga gatcatttcc cacatcgacg
      661 agaacatcaa gggcttcttc gtcccgggcc tcctcttcga gcggctgggg ttccgctact
      721 tcgggcccat cgacggccac gacattcctc agctcatccg cgtgttcacc cacctcaaaa
      781 cgctgcccgg accgctcctg ttgcacgttt tgaccgtgaa aggcaagggc tatgttccgg
      841 cagaacatga tccggcgaag ttccacggcg tgggggcctt cgataaggtg accggcaaag
      901 cgaatgccgc gggcgtaaca gcctatcagg cggtcttcgg ggagacgctg gtcaaaatcg
      961 ccgagaaaaa ttcgaaggtg gcggcgatca ccgcggccat gtgcaccggt acggggctgg
     1021 tcgaattcgc caaaaagttt ccggagcgct tctttgacgt gggcatcgcc gaagagcacg
     1081 gggcaacgtt tgccgccggc ctggcctgcc agggaatcaa accgatctat gccatctact
     1141 cgacctttct gcagcgggct tttgaccagg tcgtccatga tgtggccctg cagaatatcc
     1201 cggtcttgtt cgcggtcgat cgcgccggtt tagtcggcga cgacggcccc acgcaccatg
     1261 gggtcttcga tctctcctac ctgcgggcta ttcccaattt caccgtggcc gccccgaagg
     1321 acggcaatga attgaaagat ctgctctggt cggccatcgg ctggcaggac ggcccggtgg
     1381 ccatccgcta cccgcgcggc gggatacccg ataaacttca ggatacggtc cgcccgagtc
     1441 cctacggcac ctggcaggta gagcgcgaag gggaagaggt ggtgattttc gccgtcggtg
     1501 caatggtgta tcccgcgttg gaagccgtta cgaggttatt agtagacggc ctttctccga
     1561 ccgtggtgaa
//
LOCUS       DBNGZA010000489         7050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2010316, whole genome shotgun sequence.
ACCESSION   DBNGZA010000489 DBNGZA010000000
VERSION     DBNGZA010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2010316"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="AC16FU_10405"
     CDS             complement(<1..817)
                     /locus_tag="AC16FU_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994818.1"
                     /translation="MFAWFGTSFPSRCGTTFFLAGFLGLTAFASPASRAADPPAELKL
                     PPHTAEILAGIDLSTREEYQKARAVFRSLVERDSTDPWGWFFLAAGLQDEMMDKENFI
                     WKEEFKQAVERTLLLAKARADRDKKDAWSLFILGNAYGYLTVFLARQGDWFSGMRAAV
                     KAKGYYEKALKINPQLYDAYVGLGNYHYWKSVRTKAFLWLPFFKDTRKQGIAELEKAA
                     RLGTFARTAACFSLMYIYIHEKRYDAALALADSLSREYPESRSFLWGQAAAYFE"
     gene            1024..1941
                     /locus_tag="AC16FU_10410"
     CDS             1024..1941
                     /locus_tag="AC16FU_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994819.1"
                     /translation="MKFQKVWVLGAVLLGVLFLTGGCSKSTNPVEATDPEALKNILTE
                     SALLVTSDVTTTTPPDTSGGYARLAGVDTVKFWWRRYTRAQRNIAIQIFPNDSAHVYP
                     YALVTITDSLYGVLHVLGRDSLGARFHDSTLFADLARRNAYFEKRFASNRLHRGWVLV
                     SVSGVVINSIPFGVSSVATTRQIQSAQVVAPSGVNKTLTEADVLALVRRDSLTFFRPG
                     DSVTVTVTTADSSDSVYLHVGEDYRYQRKPFRNNLNGTFTGTWVISTDPRFWFGPKHA
                     GIDVIKHDVLDGDLAYDSKRWGLVYVIQP"
     gene            complement(2013..2561)
                     /locus_tag="AC16FU_10415"
     CDS             complement(2013..2561)
                     /locus_tag="AC16FU_10415"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR2994820.1"
                     /translation="MKRLFVCLCGGLLLFGFGCSKSTKNQFGTLKLFLTDAPAAFDAV
                     NVAVTRISVHPAGDDDPNKWQVVSGEAQTVDLLTLQNGVEILFASHPLPAGTYTQIRL
                     EVGSGSTVVTNSQTFPLIIPSGTQSGVKLVHPFTIIGGGTTSLLLDFDAEQSVVQTGV
                     TTYVLVPVIKVVTPNTGGGNPA"
     gene            complement(2678..3205)
                     /locus_tag="AC16FU_10420"
     CDS             complement(2678..3205)
                     /locus_tag="AC16FU_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994821.1"
                     /translation="MSVRHVTVTAAFAAVTLFALLLSGVAAPPQSATVRFGLNEVGSP
                     FPPPAGHDHSTHAKDHIVPRTVVIAQGGSVTFEIDEFHQPALYRPGTAPEDITVSDAT
                     LQDLSLPCVPGGVLPKFVINDPNGRILLGPPHSCAEQTWTTPPGTFDHPGRYLVICTT
                     LPHFRNGMYGWVIVQ"
     gene            complement(3395..4321)
                     /locus_tag="AC16FU_10425"
     CDS             complement(3395..4321)
                     /locus_tag="AC16FU_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR2994822.1"
                     /translation="MFHCAFFSLGSVTEAELRSTLGTEVVIFHRFHTVPQLAKQARRS
                     NQDLIFLGSTGDLRSEITLVRQIKAQAILLVTPLVLYHPIPDRRMQLSALDSGADEFL
                     SGSWDEELFGARLRMVVAKSHRDVSVNPSSHLPGPALIDKEIEKRLKEKTPLAICYAD
                     LDNFKAYNDYYGYFYGDKIIRLTARVVRDIVYDLAPEGFVGHVGGDDFVFILPTERIE
                     AVCSQIIATFDRMIPYRYAEADRQRGYIVTHNRRGETEEFPLMTISIAVIPNVAETFS
                     HSGQFSHMLADLKKYIKTLPGSNYRIERRKKY"
     gene            complement(4400..5299)
                     /gene="xerD"
                     /locus_tag="AC16FU_10430"
     CDS             complement(4400..5299)
                     /gene="xerD"
                     /locus_tag="AC16FU_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR2994823.1"
                     /translation="MNESIITYLTSLSIARGLAANTLAGYRNDLSAYQKYLAKKRISE
                     FERVTPEVVSGYVQHLSGLGRHPRSVARAVSSLRGFHRYLVLSRKAKVDPTAILEPLR
                     LPRKLPAVLDQNEVEKILEQPDNTPLGIRDGAILEYLYATGVRISELIGTRTRDLILE
                     AGFVRVFGKGKKERVVPIGSKAIKAVNDYLDDVRPTLANRKSADTVFLNARGGPLSRM
                     GAYNIILRYVRQAGIAKKVSPHTFRHSFATHLLEGGADLRVVQALLGHADISTTEVYT
                     HLDRAYLKEVHRTFHPRESNKKR"
     gene            complement(5371..>7050)
                     /locus_tag="AC16FU_10435"
     CDS             complement(5371..>7050)
                     /locus_tag="AC16FU_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002752900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR2994824.1"
                     /translation="RPLTLAYVVGRLQNINLTKTPGDIKGEAFQSFVYRHQRGDRGEF
                     FTPHPIVRLAVEMIAPKPDEKVIDPACGSGGFLVQTIAYVHRNYPNIEKAAYIQEHIS
                     GIEFNPDVALSAMIRLAFEGGTGTEITCTNSLTENERLNKAFDVVLTNPPFGSKGKVE
                     DQKILKSYVLARRWNKLSNNDWEPTKTVLPGQSPEILFIEKCLNLLRPGGRMAIVLPD
                     GLLQNISNSHIRFWIRAQAKVLGVVSIPQEAFVPYGTGIKTSLLLVQKLPASSRGDCF
                     MARIQKIGYDVKGQLLYRRDESGRLIRSSSGLPVVDDDVEEIATTFNAFKKDGALCQN
                     RENIYTASEEFLNSRMDAEHYQPDDKKLIEHIRSLGAKPLGEIADILTETDDFRLESD
                     SEIRYIAISDVDARTMQVVSQQIIKAHEAPSRATYRLRKGDIITASSGASTGTQRQAT
                     ALITEDEDGAICSNGFAVIRNIQGVQPLFLLAYMRTEYYLRQVRRLMTGHAIPSISIE
                     DLAKVLVPILPKKEQERIANSIASIQLMRKDALKAGEKVVMETHALISQTS"
BASE COUNT         1518 a         1909 c         2017 g         1606 t
ORIGIN      
        1 tttcgaagta cgcggcggcc tggccccaga ggaaactcct gctctccggg tactcgcggg
       61 aaagactgtc ggctaaggcc agcgccgcgt catagcgttt ctcgtgaatg taaatgtaca
      121 tcaaggagaa gcaggcggcg gtccgggcaa aggtccccag ccgcgccgct ttttccaatt
      181 cggcaattcc ctgtttgcgc gtatctttga aaaaaggcag ccagagaaaa gctttcgtcc
      241 gcacgctctt ccagtagtgg tagtttccta aaccgacgta ggcgtcgtag agctgcgggt
      301 tgattttcag ggctttttca tagtaccctt tggccttcac cgccgcgcgc atacccgaaa
      361 accaatcgcc ctgccgggcc aaaaagacgg tcaggtagcc gtaggcgttt cccagaatga
      421 aaagactcca ggcgtctttt ttgtcgcggt cggcccgcgc tttggctaaa agcagggttc
      481 gctcgacggc ctgtttgaat tcctccttcc agatgaaatt ttctttgtcc atcatctcgt
      541 cttggaggcc cgccgccagg aaaaaccagc cccagggatc ggtggaatcg cgttcgacga
      601 gcgagcggaa gacggcccgg gctttctggt attcctcccg ggtggagagg tcgatgcccg
      661 ccagaatttc ggcggtgtgg ggaggcagtt tcagttctgc gggcggatca gcagcgcggg
      721 aggcgggcga agcgaaggcc gtcaggccga gaaaaccggc caaaaaaaaa gtcgtgccgc
      781 agcgcgaggg gaaagaagtt ccgaaccaag cgaacattgg tcggccaagt aaaacggctg
      841 aggatggaaa agcaaatggc tgtggggcat cggaaggccg gagataaaga agcccgcgca
      901 aatggccgat attaggttgc gtagcctacc ggaagtattt ttctcttgcg ccgccgaaaa
      961 acacgaacgg attaaacccg gcgggccggg accggtctaa ttgatgatag caaaggaggc
     1021 gggatgaaat tccaaaaagt ctgggtgctg ggagcagttc ttctgggtgt tctgtttttg
     1081 accggcggtt gcagcaaatc gaccaacccg gtggaggcga ccgacccgga agccctgaag
     1141 aatatcctga ccgagagcgc gcttctggtc acttccgacg tgacgaccac cacgccgccc
     1201 gacacctccg gcggctacgc gcggctggcc ggggtcgata cggtcaagtt ctggtggcgc
     1261 cgctacaccc gggcgcaaag gaatatcgcc attcaaattt tcccgaatga ttccgcgcac
     1321 gtctacccct acgcgctcgt gaccatcacc gattcgctct acggtgtcct ccacgtttta
     1381 ggccgggact ccctgggcgc gcggttccac gattcgaccc tctttgccga tctggcccgg
     1441 cgcaacgcct attttgaaaa gcgcttcgcc tccaaccggc tgcaccgcgg ctgggttctg
     1501 gtgagcgttt ccggcgtggt gatcaactcg attcccttcg gcgtctcttc ggtcgccacc
     1561 acccggcaga ttcaatcggc gcaggtcgtc gctccctccg gcgtgaacaa aaccttgacc
     1621 gaagctgatg tcctggcctt ggtccggcgc gattccctga cctttttccg tcccggggac
     1681 agcgtgacgg tgacggtcac gaccgccgat tcttccgatt cggtctacct gcacgtgggg
     1741 gaggactacc gctaccagcg gaaaccgttc cggaacaacc tcaacggcac cttcacgggc
     1801 acctgggtca tctccaccga cccgcgcttc tggttcgggc cgaagcacgc cggcattgac
     1861 gtgatcaagc acgacgtgct ggacggcgac cttgcctacg acagcaagcg ctggggtctg
     1921 gtctacgtca ttcagccgta agaaattgag caagcagcaa aaagtccccg tacgggcact
     1981 ttttggtttt cggataagat aatgtgtaga tcctacgccg gatttccccc gcccgtattg
     2041 ggggtgacga ctttgatgac cgggacgagg acgtaggtcg tcacgccggt ctgcaccacc
     2101 gactgctcgg catcgaagtc gagcaagaga ctggtcgtcc cgccgccgat gatggtgaaa
     2161 ggatgtacca gtttcacccc gctctgggtg ccactgggga taatcagggg aaaggtctgg
     2221 ctgttcgtaa ccaccgtcga cccgctgccg acttcgaggc gaatttgcgt ataggtgccc
     2281 gccgggaggg gatgggaagc aaaaaggatt tcgaccccgt tctgcaaagt gaggagatcg
     2341 acggtctggg cctcgcctga gaccacctgc cacttgttcg ggtcgtcgtc tcccgccgga
     2401 tggaccgaga tccgcgtgac ggcgacgttc accgcgtcga aggccgcggg cgcgtcggtc
     2461 aggaagagtt tcaatgttcc gaactgattc ttggtgcttt ttgagcaccc gaaaccgaat
     2521 agaagcaagc ctccacaaag gcagacgaaa agccgtttca tcgcatacct cccatcatca
     2581 ttcagggaat acgatactac ttttcccgaa aatagcaagt attttctcag caccagcccg
     2641 gaagggaggt gcaaagtccg ggccagggcg gacatcccta ctgcacaatg acccagccgt
     2701 acatgccgtt ccggaagtgg ggcaaggtcg tgcagatgac gaggtagcgt cccgggtggt
     2761 cgaacgtccc cggaggcgtg gtccaggtct gctcggcgca agaatgcggc ggacccaaaa
     2821 gaattctgcc gttggggtcg ttgatcacaa acttcggaag aacgcccccc ggcacgcagg
     2881 ggagcgagag gtcctgcagg gtggcgtcgc tgacggtgat atcctcgggt gccgtccccg
     2941 gacggtagag ggccggctgg tggaactcgt cgatttcaaa ggtcacgctg ccgccctggg
     3001 caatgaccac cgtgcgcggc acgatgtgat ccttggcgtg cgtggagtga tcgtggcccg
     3061 cgggcggggg aaagggagaa cccacctcgt tcaagccaaa gcgtacggta gcactctggg
     3121 gcggcgccgc tactccgctc aaaagcaggg cgaaaagcgt gaccgccgcg aacgcggctg
     3181 tgacggttac gtgtctcact gacatgactc ctcctccttc tcctctggaa cgtgcgaact
     3241 ttagagattt atacggaagc cgtgacaggc cgttagcggc atcccggatg ccggtcaaaa
     3301 aagatgataa ggtatttcaa tgacctgcct ggggcgactg ccaaaactac gcgggtgtca
     3361 ccgagaacca ttccccaaaa ggtttttcta agaatcaata ctttttgcgg cgctcgatcc
     3421 ggtagttcga ccccgggagg gttttgatgt acttcttcaa gtcggccagc atgtgggaga
     3481 actgtccgga gtgggagaag gtctcggcca cgttggggat gaccgcaatc gaaatcgtca
     3541 tcagggggaa ctcttcggtc tcgccccggc ggttgtgggt gacgatgtac cccctttggc
     3601 ggtcggcctc ggcgtagcgg taggggatca tccggtcgaa ggtggcgatg atctggctgc
     3661 agacggcctc gattcgctcc gtcgggagga tgaagacgaa atcgtcgccg cccacgtgcc
     3721 ccacgaagcc ttccggcgcc aggtcgtaga cgatgtcgcg caccacccgc gcggtgagcc
     3781 ggatgatctt gtcgccgtag aagtaaccgt agtagtcgtt atacgctttg aaattgtcca
     3841 gatcggcgta gcagatcgcc aagggcgttt tttccttcaa gcgtttttcg atttccttat
     3901 cgatcaaggc cgggccgggg agatgcgaac tgggattgac cgagacgtcg cggtggcttt
     3961 tggccaccac catccggagg cgcgcgccga agagttcctc gtcccaggag ccggagagaa
     4021 attcatcggc cccggaatct aaagcagaca actgcatccg gcggtcgggg atcgggtggt
     4081 agaggaccaa aggcgtcacc agaaggatcg cctgggcttt aatctgccgg acgagggtga
     4141 tttcggagcg caggtcgccg gttgagccga ggaagatcaa atcctgattc gaccgccttg
     4201 cctgcttggc caactggggc acggtgtgga agcggtggaa gatgacgact tcggtcccta
     4261 aagtcgaacg cagttcggct tcggtgaccg atcccagaga gaaaaaggca caatggaaca
     4321 taggcgcagg gctacacgca caccggttcg ctctgaaccc accgaccgtg cccctggcta
     4381 tccctgccgc tcgaaagtcc tatcttttct tgttggactc ccgcgggtgg aaggtccggt
     4441 gcacctcttt caagtaggca cggtccaggt gcgtgtagac ctcggtagta gatatatcgg
     4501 cgtggcccag gagcgcctgc acgaccctga ggtcggcgcc gccttcgagc aaatgggtgg
     4561 caaaggagtg ccggaaggtg tggggggaga cctttttggc aattcccgcc tggcggacgt
     4621 agcgcagaat gatgttgtac gcccccatcc gggagagcgg cccgccccgg gcgttcaaaa
     4681 agacggtatc cgctgatttc cggttcgcga gcgtggggcg gacgtcgtca aggtagtcgt
     4741 tcactgcctt aatcgctttg gagccgatcg gcacgaccct ttcttttttc cctttcccga
     4801 agacgcggac gaagccggct tctaaaatca aatccctggt gcgggtgccg attagttccg
     4861 aaatccttac tccggtggcg tagaggtatt ccaaaatcgc cccgtcgcgg atgcctaagg
     4921 gcgtattgtc cggctgttcc aagatttttt ccacttcgtt ttgatcgagc accgccggca
     4981 gtttgcgagg caggcggagc ggttccagaa tcgccgtggg atcaactttc gctttccggg
     5041 agaggaccaa ataccggtga aacccgcgca gactggaaac cgcccgcgcc accgagcggg
     5101 gatggcggcc cagacccgaa agatgctgca cgtagccgga gaccacctcc ggcgtcaccc
     5161 gttcgaattc gcttattctt tttttggcga gatatttctg gtaggcgctc aggtcgttcc
     5221 ggtaaccggc cagcgtattc gccgccagcc cgcgggcgat ggaaagggag gtgaggtagg
     5281 tgatgatgga ttcgttcatg gcatcccgat aaatataaag aaaaaggcgc tccttcgggg
     5341 ccgccctatt ttgatttgcg aatttactta tcaactcgtt tgacttatca aagcgtgggt
     5401 ttccattaca accttttctc ctgctttcag cgcgtcctta cgcattaact gaattgatgc
     5461 aattgagtta gctattcttt cctgctcctt cttgggaaga atcggcacta gaactttcgc
     5521 taaatcctct attgatattg aaggaatggc atgacccgtc attagccttc taacctgtcg
     5581 caagtaatat tcggtacgca tatacgccag caagaatagg ggctgtactc cttgaatgtt
     5641 ccgtattaca gcaaaaccgt ttgaacaaat cgctccgtct tcatcttcgg taataagtgc
     5701 tgtggcttga cgttgtgtgc ccgtactcgc tcctgagcta gccgtgatta tgtcgccttt
     5761 acgaagccga taagttgccc tggatggggc ttcatgcgct tttatgattt gctgcgaaac
     5821 aacctgcatc gtacgtgcgt caacatctga aatggcgata tatcttatct cgctatcgct
     5881 ttctagtctg aaatcgtcgg tctcagtcag aatatctgct atttcgccaa gaggctttgc
     5941 gccaagcgat cttatatgct caatcagttt tttgtcatca ggttggtagt gttcagcgtc
     6001 catacgcgaa ttaagaaatt cttctgaggc ggtatagata ttttctctgt tttggcaaag
     6061 tgccccatct tttttaaagg cattgaatgt ggttgctatt tcttctacgt catcatcaac
     6121 aacaggtaaa cccgatgatg atctaattaa tcttccagac tcgtcgcgcc tatatagtaa
     6181 ctgacctttc acgtcatagc caatcttttg aatacgagcc ataaaacaat cgccacggct
     6241 actagcaggc agtttttgga ccagtaaaag cgatgttttt atgcctgttc cgtagggaac
     6301 gaatgcttct tgtggaatag atacaacccc taagactttg gcctgcgcac gaatccaaaa
     6361 tcttatgtgg ctgttagaaa tgttttgaag taacccatca ggcagaacaa tcgccatgcg
     6421 tcccccgggg cggagcaaat tcaggcattt ttcaataaag agaatttctg gcgattgtcc
     6481 gggcagtacc gtttttgtag gttcccaatc gttatttgat aacttattcc acctgcgcgc
     6541 taagacatac gacttgagga ttttctgatc ttctactttg cccttactgc caaaaggggg
     6601 attagttaga actacatcga aagccttgtt cagtctctcg ttttccgtga gagaatttgt
     6661 acaggtaatt tctgttccag ttccgccttc aaaggcaagt cgaatcattg ccgacaaagc
     6721 gacatcgggg ttgaattcaa tcccagagat atgttcctga atgtaggcgg ccttttcaat
     6781 gttgggatag tttcgatgca cgtaagcaat cgtttgaaca agaaatccgc cactaccaca
     6841 agccgggtca ataacttttt catcaggttt aggtgctatc atttctacag caagtctgac
     6901 gattgggtga ggagtgaaaa actcaccgcg gtcgcccctt tgatggcggt atacaaatga
     6961 ttgaaaggct tcccctttaa tgtctccggg cgttttagta aggttgatat tctgtagtcg
     7021 cccgaccaca taggccagtg tcaggggcct
//
LOCUS       DBNGZA010000490         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_98023, whole genome shotgun sequence.
ACCESSION   DBNGZA010000490 DBNGZA010000000
VERSION     DBNGZA010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_98023"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="mnmA"
                     /locus_tag="AC16FU_10440"
     CDS             <1..932
                     /gene="mnmA"
                     /locus_tag="AC16FU_10440"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR2994825.1"
                     /translation="EDARFAARKLGFPHYVFDYTVPFGKAVIDDFASEYLAGRTPNPC
                     VQCNSKIKWAPLLERALALNCDYLATGHYAQVAFDEDRERFVLRKASDLFRDQSYFLW
                     RLTQEQLARTLFPLGALPKAEVRTVAHRLNLRNADKPDSQEICFVTDNNYQRFLRERL
                     AKAGVAMTPGPIYDTGGRKLGEHQGVAFYTVGQRRGLGVAVGEPLYVVAIDPRENAVI
                     VGKDDELRRRSFTVSQVNWVSIPEPKEALRCNVRIRYRHREAPATVTVTGSDSVQVEY
                     DVPERAITPGQSAVFYDGDLVVGGGIIESASGG"
     gene            953..1633
                     /locus_tag="AC16FU_10445"
     CDS             953..1633
                     /locus_tag="AC16FU_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR2994826.1"
                     /translation="MAKLTWEGHACFKIETGKHNIWIDPFLSGNPLAKTKPEQVKADF
                     ILVTHGHGDHLGDTIAIAKRTKATVIAPFELAMYCQMHGAPNVHPMHIGGGHHFPFGH
                     VKLTIAHHGSAMVDKEITYTGNPCGFLVTSEGKTIYHAGDTGLFGDMALIGQMQKIKA
                     ALLPIGDNFTMGPDDAIKAVELLKAEIVIPMHYNTFEVINQNPQEFVRKVKGATVVIL
                     EPGKSFEI"
     gene            1688..>2996
                     /locus_tag="AC16FU_10450"
     CDS             1688..>2996
                     /locus_tag="AC16FU_10450"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR2994827.1"
                     /translation="MKRRAKIFLWVIGLLVALLLVGAAVLKLIFTRERILALVLPKVE
                     QAVGRKVTVGDASLSIWGGLGLELSQVTVANRKAFSSPYLATLDGLDVRVRFWPLLRK
                     QLDVTKLILNLPQIFLEKNKKGQTNYAGLFQSRTPEAAAKKTDSLLAVAFDRLEIKEG
                     RVIWRDDSTQTLAALTDLSARLALARVGASGGRLSGQLAAGVQYEKGLKKFSSGRAPI
                     TLEPEVIFDLAREQVTLNSVKLTWGKVALAGSGNLSGWQRPAFDLKFLSSRINVGDLW
                     QTLVTSFPEKLAQAQAAGFLQLDLEVIGRKGEATGPHLSGNLLLENVELKTAETPAPL
                     RIRSGLVDFSPDELRISTQSASFGDSPLKVLATVTDFKDPRLSADLDFNLDLRFLSGL
                     SQAKDKTKFWGTLSGRLLANGKMKQPELLNLSGSVQARDVGYQT"
BASE COUNT          632 a          865 c          876 g          623 t
ORIGIN      
        1 ttgaggacgc tcgcttcgct gcaaggaaac tcggatttcc tcactacgtt ttcgattaca
       61 ccgtgccctt cggcaaggcg gtgattgatg actttgcctc tgaatatctg gccggtcgga
      121 cgcccaaccc ctgcgtgcag tgcaattcga aaatcaagtg ggccccgctg cttgagcgag
      181 ccctagccct gaattgcgat tacttagcga ccggccatta cgcgcaggtg gccttcgacg
      241 aggaccgcga gcgcttcgtt ctgcggaagg cctccgacct tttccgcgat cagtcatact
      301 tcctctggag actgacgcag gagcagttag ccaggacgtt gttccctctg ggtgcgcttc
      361 ccaaagcaga agttcggacg gtggcgcatc gtctcaatct ccgcaacgcc gacaagccgg
      421 acagccagga gatttgcttc gttacggaca acaactacca gcgttttctc cgcgagcggc
      481 tggctaaggc gggcgtggcc atgaccccgg ggccgattta cgacaccggc gggcgaaagc
      541 tcggagagca tcagggcgtg gcgttctaca cggtgggtca gcggcgagga ctcggagtgg
      601 cagttggaga gccgctttac gtggtggcga ttgatccaag agaaaatgcg gtcatcgtcg
      661 gcaaagatga tgaattgcgg aggcgaagtt ttaccgtatc gcaggtgaac tgggtttcca
      721 tcccggagcc caaagaagcg ctccgctgta acgtacgcat ccgctaccgg caccgggaag
      781 ctccggctac agttaccgtt accggatcgg attctgttca ggtggagtat gacgtaccgg
      841 agcgggcgat tacgccgggc cagtcggcgg ttttttacga tggtgacttg gtggtaggcg
      901 gtgggattat cgaatccgcc tcaggcggat agattttcgc tatggagggc agatggccaa
      961 actgacgtgg gaaggacatg cctgtttcaa aatcgaaacc ggcaagcata acatctggat
     1021 tgatcccttt ctctccggca atccgttagc caagaccaaa cccgagcagg tcaaagccga
     1081 tttcatctta gtgacccacg ggcacggcga ccacttaggc gacaccatcg ccattgccaa
     1141 gcgaacgaag gccaccgtca ttgcgccttt tgaactggca atgtactgcc agatgcacgg
     1201 tgcgcccaac gttcacccga tgcacatcgg cggcgggcac cactttccct tcggccacgt
     1261 caaactcacg attgcgcacc acggctcagc catggtcgat aaggaaatca cctacacggg
     1321 gaatccctgc ggctttctgg tcaccagcga aggcaaaacg atctatcacg cgggcgacac
     1381 cggtcttttc ggcgatatgg cgctcatcgg ccaaatgcag aagatcaaag cggcgctttt
     1441 gcccatcggc gacaacttca ccatgggccc ggacgacgcg atcaaagccg tggaactgct
     1501 taaagccgaa atcgtcattc cgatgcacta caataccttc gaggtcatca accagaaccc
     1561 ccaggagttc gtccgtaagg tgaaaggcgc gacggttgtc attctggaac cgggcaagag
     1621 ttttgagatt tagttgccga tagacctgaa gtcggcagtc tggggttgga gttcgtgagt
     1681 tccaacgatg aaacgtagag ccaaaatttt tctctgggtc atcgggcttc tcgttgccct
     1741 cctgcttgtg ggcgcggcag tcctgaaact cattttcacc agagagcgca ttctggctct
     1801 cgtgttgccc aaagtggaac aggccgttgg tcggaaagta accgtcggcg acgcatccct
     1861 ttcgatttgg ggcgggctgg ggttggaatt gagccaggtc accgttgcca accggaaggc
     1921 gttctccagt ccgtacctgg cgacactcga cggcttggat gtgcgcgtgc gcttttggcc
     1981 gcttttgaga aagcaacttg acgtcacgaa gttaatcctc aacctcccgc agatttttct
     2041 ggaaaagaac aaaaagggac agacgaatta cgccggtctg tttcagagcc gaacgccgga
     2101 agcggcggcg aaaaagaccg actccctttt ggcggttgcc ttcgaccggc tcgaaatcaa
     2161 ggagggccgg gtgatctggc gggatgattc cactcaaacg ctcgccgccc tgaccgatct
     2221 ttccgcccgg ctggcgctgg cgcgcgtcgg cgcttccggc gggcggcttt cggggcagct
     2281 cgcggccggt gtgcagtacg aaaaagggct aaagaaattt tcctccggtc gcgcccccat
     2341 aacgcttgag cccgaggtga ttttcgatct ggcgcgggaa caggtcaccc tgaatagcgt
     2401 caagctcacc tggggcaagg tggcgctcgc cggctccgga aatctttccg gctggcagcg
     2461 tccggccttc gacttgaagt tcctttccag ccggatcaac gtgggcgatc tgtggcagac
     2521 tctggtcacg agttttccgg aaaaattggc ccaagcgcag gccgccggct ttttgcaact
     2581 cgatctggag gtcatcggac gcaagggcga agccacaggc ccgcacctat ccggcaatct
     2641 gcttctcgaa aatgtcgaat tgaaaaccgc cgaaacgccg gcgcccctgc gtattcggag
     2701 cggactcgtc gacttcagcc cggacgaact ccggatttcg acccagtcgg cttccttcgg
     2761 cgactcaccg ctcaaagtac tggcgacggt caccgacttc aaagacccgc gcctgtcggc
     2821 ggatctggac ttcaacctcg acctgcgctt cctttccggt ctctctcagg cgaaggacaa
     2881 aacgaagttc tggggcaccc tttccggtcg attgttggcg aatggaaaaa tgaaacagcc
     2941 ggagttgctc aatctctccg gctcggtaca ggcccgggac gtgggctacc agacca
//
LOCUS       DBNGZA010000491         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_310478, whole genome shotgun sequence.
ACCESSION   DBNGZA010000491 DBNGZA010000000
VERSION     DBNGZA010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_310478"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1361)
                     /locus_tag="AC16FU_10455"
     CDS             complement(39..1361)
                     /locus_tag="AC16FU_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR2994828.1"
                     /translation="MKKRSKILLIAGGALVVIIVVVLNLVKSGSAVVKVQTEKVEKRD
                     ITSIVTATGKVEPKTTVKISANISAKITRLPVKEGDRVKKGQVLVQLEPTRYQAAVNQ
                     YAASLAAARAQLRLAESKLAESRQLRDRTQSLFEKNLASKEQYDAVVTAYAVAEATVD
                     NYQHQVEQTEAALAQAQDDREKTTITAPMNGVVTELKAEAGEVVIPGTMNNPGTVILV
                     VSDLSEIEVKVDVDETDIALVKLGQPAKIEIDAFPDTAYHGRVTEIGNSAQVKGLGSQ
                     DQVTNFQVKVLLVDTVPNIKPGMTASVDVTTDSRSGVVAVPIQAVVMREEKDLKKKST
                     AEDRKKAKQGETVAEAAPVDTAAGKNPKGKKAKPLEGVFVVREGTAHFVSVKTGISDQ
                     ENIEIASGLEPGDEVVVGSYKILRTLEDEAKVKKEVMTASAAAGEEKQ"
     gene            complement(1447..2772)
                     /locus_tag="AC16FU_10460"
     CDS             complement(1447..2772)
                     /locus_tag="AC16FU_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994829.1"
                     /translation="MRKPLSGIFVLTLIVSGTAAAQEAPASGGLTLDECIKIALKENP
                     TVIAAQHSYTAAKGEVYRAWGGLLPSVNLGLDMSFFKSGFPRINQATGQFQQARWSLT
                     YRPSVSLNQTLFNGGANYYNVAWRADEKEAAYQALQSTRQQMVLSVKENYFALLKAKM
                     LLDIQKDAVKRGEEQLKVAQTRYDLGSASLSDVLKAKVLYGNDQLALVSAENSYELAK
                     ANLNFILNRDVNTPVEIEEKLPDVQNPYTLEQALAQAKISHPGLRQARADYSASRAQV
                     GIARAAYVPEVSWGLTRSWSVPDTRVERLLKFDQVYGTWSVFISANFPLFNGFNREAT
                     FLASRVNSDTKKENLARTENQVALEVKQAFLGIAESQKKLQVSEENEKSSQEDLNLAK
                     EKYNLGAATILEVLDAEVNFKTVQSNRVQALFDYHLAIANLEKAMGRKY"
BASE COUNT          517 a          905 c          794 g          734 t
ORIGIN      
        1 attttccgtg cggatgaggg cttctttacc gttctgcatt actgcttctc ctcgccggcc
       61 gcagccgacg cagtcatcac ctcttttttt accttcgctt cgtcttcgag ggtgcgcagg
      121 attttgtaac taccgaccac cacttcgtca cccggctcga ggccggaagc gatttcgata
      181 ttttcctgat cggagatgcc ggttttcacc gacacgaaat gggccgtccc ctcccgcacc
      241 acgaacacgc cctccaacgg tttggccttt ttgcccttcg ggtttttgcc agccgcggtg
      301 tccaccggtg ccgcctcggc cacggtctcg ccttgcttgg ccttcttgcg gtcctccgcc
      361 gtcgattttt tctttaaatc tttttcttcc cgcattacca cggcctggat cgggaccgcc
      421 accaccccgg agcgggagtc ggtggtgacg tccaccgagg cggtcatgcc gggcttgata
      481 ttgggcaccg tgtctacgag cagaaccttc acctggaagt tggtcacctg gtcctgcgag
      541 cccaaccctt tcacctgggc cgagttgccg atttcggtca ccctgccgtg atacgcggtg
      601 tccgggaaag cgtctatttc gatcttcgcc ggctggccca gtttcaccag cgcgatgtcg
      661 gtttcgtcta catccacttt gacttcgatt tcagagagat cggagaccac caggatgacc
      721 gtgcccgggt tattcatcgt gccggggatc accacttcgc ccgcctcggc cttgagctcg
      781 gtgaccaccc cgttcatcgg cgcggtgatc gtggttttct cccgatcgtc ctgggcctgt
      841 gctaaagccg cctcggtctg ctccacctga tgctggtagt tgtcgacggt ggcttcggcc
      901 accgcatagg cggtcacgac cgcatcgtat tgctctttcg acgccaggtt tttttcgaaa
      961 agcgactggg tccggtcgcg caattgccgg gattcggcca gcttcgattc ggccagccgg
     1021 agctgcgccc gcgccgccgc cagagacgcg gcgtactggt tcaccgccgc ctggtagcgt
     1081 gtcggctcca gctgcaccag cacctggcct ttcttcaccc ggtccccttc tttgaccggc
     1141 aggcgggtga ttttggccga gatgttggcc gaaattttca ccgtggtttt cggctccacc
     1201 ttgccggtgg cggtgacgat ggaggtgatg tcccgcttct ccactttttc ggtctgcacc
     1261 tttaccacgg cactccccga tttgaccagg ttcagcacca ccacgataat gacgaccaaa
     1321 gccccgccgg caatcaagag tatcttactt cgtttcttca tctctctctc cttaagaata
     1381 cggcaaaacc gtcattgcgt gctctgcctg cagaaactgc gccgggcaat ttccgcaagc
     1441 aaaaaatcag tatttcctcc ccatcgcttt ttctaaattg gcaatggcca gatggtagtc
     1501 gaacaaggcc tgcacccggt tcgactgcac ggttttaaag ttcacttccg catccagaac
     1561 ttccaggatg gtcgccgcgc ccaggttgta cttctccttg gccaggttca agtcttcctg
     1621 cgacgacttt tcgttctcct cgctcacctg gagtttcttc tgcgactcgg cgatgcccag
     1681 aaacgcctgc ttgacttcca gcgccacttg attttctgtc cgggctaaat tttccttttt
     1741 tgtgtcgcta ttgacccggc tggccaaaaa ggtcgcctcc cggttgaacc cgttaaagag
     1801 tgggaagttg gccgagatga agacactcca ggttccgtac acctggtcga atttcaaaag
     1861 ccgttcgacg cgggtatccg gcaccgacca ggagcgggtg agcccccagg agacctcggg
     1921 aacgtaggcg gcgcgggcga tgcctacctg tgcccggctg gcgctgtagt cggcccgcgc
     1981 ctggcgcaat ccggggtggg aaatcttcgc ttgcgctaag gcctgctcca gggtgtatgg
     2041 attctggaca tcggggagtt tttcttcgat ttccaccgga gtgttcacgt cgcggttcag
     2101 gatgaaattg agattcgcct tggccagctc gtagctattt tcggcggaca ctaaggccag
     2161 ttggtcattg ccgtagagca ccttcgcctt caaaacgtcc gaaagcgacg ccgagccaag
     2221 atcgtagcgc gtctgggcca ctttcaactg ttcctcgccg cgcttgaccg catccttctg
     2281 aatgtccaaa agcatcttgg ctttgaggag cgcgaagtag ttctctttga cgctcaaaac
     2341 catttgctgg cgggtactct gcaaggcctg gtaggcggct tctttttcat ccgcgcgcca
     2401 ggctacgttg tagtagttag cgccgccgtt gaaaagcgtc tggttgagcg agacgctggg
     2461 ccggtaggtc agactccagc gcgcttgttg aaactgcccg gtcgcctggt tgatccgggg
     2521 gaacccggac ttgaagaagc tcatgtccaa ccccaggttt accgagggga gcagcccgcc
     2581 ccaagcccgg tagacttccc ctttggcggc ggtgtagctg tgttgggctg caatgacggt
     2641 cgggttttcc ttgagcgcaa ttttgatgca ctcatccagg gtcaacccgc ctgaggcggg
     2701 cgcttcctgg gcggctgccg tcccggacac gatcaaagtg agaacaaaaa tgccactcaa
     2761 tggttttcgc attcagactc ctcttggttc tttcttttat cctttacgga tgaccgaccg
     2821 cgtaagttcc aatcaagggt cacgaccaac gaaaaatcgt cttcctacgc attctcctcc
     2881 tcgtttatgc aactaaaaag atagccggga cagtcctccg gagaaataac tcaaggcggc
     2941 cttaataatc
//
LOCUS       DBNGZA010000492         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2011371, whole genome shotgun sequence.
ACCESSION   DBNGZA010000492 DBNGZA010000000
VERSION     DBNGZA010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2011371"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16FU_10465"
     CDS             <1..909
                     /locus_tag="AC16FU_10465"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR2994830.1"
                     /translation="DGQEPAEPVVVKGHRAHTRGLVKIQDGCNEWCAYCVIPMSRGDS
                     RCRPAEQITAEVRSLVEHGFKEVVITGVHIGKYRHDELDLVGLLRRILDETDVARLRL
                     SSIKPNEVTPELIDLIASSGRICRFVHMPLQSGDDTTLKRMKRSYDCATYLRIAERLK
                     EKIPGLTIGADVIVGFPGETDEQFENGARFIEDSPIDYLHVFSYSDRPNTAASRMPDK
                     VPPQVKDHRSFVLRQLSDKKWKCHLDRFIGQRMPVLVEENGKVASCKTAIADNFIRVL
                     LPENGYKENSIVEVIPNKREGKSLVA"
     gene            1186..1743
                     /locus_tag="AC16FU_10470"
     CDS             1186..1743
                     /locus_tag="AC16FU_10470"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2994831.1"
                     /translation="MIITVKPLDDSLRNDFFTLHSDPAFGWCFCVAWEVPTWDGWGER
                     TAEQNRTLRENLFNQSRFDGYLLYVDGQPVGWCQCGPLEHWPKLYQQYQLKPEPKDYA
                     VTCFCLLPQYRKQDLSHLLLKEVLAHLKKRKVARVFAFPRKGENPDDDVWTGPEKIFL
                     SAGFTLWREAGKRVVLVRQLKSTAP"
     gene            1815..>2233
                     /locus_tag="AC16FU_10475"
     CDS             1815..>2233
                     /locus_tag="AC16FU_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR2994832.1"
                     /translation="MTLLSKHLYIETYGCQRNAAAAALSDGHLASAGYRPTDSVTDAA
                     GSLVNTCAVREHAEQRVLGRVSQLAALKTQNPDLILGLVGCMATHLREKLLDKAPFLD
                     LVVGPESYRRLPQIIDEAADQPYVDVRLDKYETYADIT"
BASE COUNT          474 a          671 c          647 g          441 t
ORIGIN      
        1 gacgggcagg agccagccga gccagtcgtc gtcaaaggcc accgcgccca cacgcggggg
       61 ttggtcaaaa ttcaggacgg ctgcaatgag tggtgcgcct actgcgtcat cccgatgtcc
      121 cgcggcgact cccgctgccg cccggcagag cagatcacag ccgaggtacg gtcgctggtc
      181 gagcacgggt tcaaagaggt ggttatcacc ggcgtccaca taggcaagta tcgccacgat
      241 gagctcgatt tggtcggcct cctgcgccgg atattggatg agaccgatgt cgcccgcctg
      301 cgcctttcgt ccatcaagcc gaatgaggtt acgccggaac tgattgatct cattgcgtcg
      361 tctggacgga tttgccgttt tgtccatatg cccctgcagt cgggggacga taccaccctc
      421 aagcggatga agcgcagtta cgattgcgcc acgtacctgc ggattgcgga acggctcaaa
      481 gagaaaattc ccgggctgac catcggcgcc gatgttatcg tcggcttccc cggcgagacc
      541 gatgagcagt tcgagaatgg cgcacgtttc attgaggatt cccccatcga ctacctgcac
      601 gtcttctcct actccgatcg gcccaatacg gcggcgtcgc gcatgcccga caaagttccg
      661 cctcaggtca aagatcaccg cagtttcgtc ctgcgccagt tatctgacaa gaaatggaaa
      721 tgccaccttg accggttcat cggccagcgc atgccggtgt tggtggagga aaacggcaaa
      781 gtcgcttcct gtaagaccgc catcgccgat aacttcatcc gggtgcttct ccccgagaat
      841 ggatacaaag agaattcaat tgtggaggtg attccgaaca agcgagaggg gaagagcttg
      901 gttgcctgag agataaagtt tccactgaga ttcccttccc tatgagggtc gaagaccccg
      961 cctctggccg tacggcccat atgggcgggg gagggtaggg aggggtcagc ccgcaggcaa
     1021 agtcataacg catacctccg accccgcccg tcccgccttt ggcgggacac cctcccctga
     1081 aggagaggga aaatgctgac ctcatcccga ccctctccta ttaggagagg gagcaactta
     1141 ccctcccctt gaggggaggg tagggagggg tcaactgaga acgccgtgat aatcaccgtc
     1201 aaacccttag acgactccct ccgcaacgac ttcttcaccc tccactccga ccctgccttc
     1261 ggctggtgct tctgcgtggc ctgggaagtg cccacctggg atggatgggg cgagcggact
     1321 gccgagcaga accgaactct acgcgaaaat cttttcaacc agagccgctt tgacggctac
     1381 ctcctctacg tcgatggtca acccgtcggc tggtgccagt gcggcccgct cgagcactgg
     1441 ccgaagctct atcaacagta tcaactcaag ccagagccca aagattacgc cgtcacctgt
     1501 ttttgcttgc ttccccagta tcgaaagcag gatttgagcc acctactctt gaaggaagtt
     1561 ctcgctcatc tgaaaaaacg gaaggtggcg cgggtctttg cctttccccg aaaaggagaa
     1621 aatcccgacg acgatgtctg gacggggcca gagaagattt ttctctccgc aggatttacc
     1681 ctctggcgcg aggcggggaa acgtgttgtg ctggtgcgac aactgaaatc aacagcccca
     1741 tgaatttccc tcccctgtag ggggggcagg gtggggtctt gcgttttgag tgtggggatg
     1801 taaactatcc ctcaatgact ctcctatcta aacacctcta catcgaaacc tacggctgcc
     1861 agaggaacgc ggccgccgcc gcgttgagcg acggccacct ggcgtccgcg gggtatcggc
     1921 cgaccgactc ggtgaccgac gcggcgggca gtctggtcaa cacctgcgcg gtgcgggagc
     1981 acgccgagca gcgggtctta ggccgggtgt cccaactggc ggctttgaaa acccaaaatc
     2041 cggatttgat tttgggtctg gtcggttgta tggcgacgca cctgagagaa aaactcttgg
     2101 acaaagcacc gttcttggat ttagtcgtcg ggcccgagtc ctaccgccgc ctgccccaga
     2161 tcatagacga agcggccgac cagccctacg tcgacgtccg gctcgacaag tacgagacct
     2221 acgccgacat cac
//
LOCUS       DBNGZA010000493         3336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2643720, whole genome shotgun sequence.
ACCESSION   DBNGZA010000493 DBNGZA010000000
VERSION     DBNGZA010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2643720"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..627)
                     /locus_tag="AC16FU_10480"
     CDS             complement(238..627)
                     /locus_tag="AC16FU_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994833.1"
                     /translation="MVVAVCLSARTLAQDQDPPNPSSCQPYGTVTIHGGPAPDSTAVV
                     ATINGVVYARGYTQGGNYVLLIAKDDSQTPQKEGWESGDLVFISVNGNLAYPSFEAFA
                     GAQQKDLTVAAAAVKLTTWGKIKAIFK"
     gene            complement(850..1521)
                     /locus_tag="AC16FU_10485"
     CDS             complement(850..1521)
                     /locus_tag="AC16FU_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR2994834.1"
                     /translation="MKVGDQKPQPRRRTIRLAEYDYSRAGAYFVTICTQARECFFGSV
                     TDGKMQLNDAGRMVERIWRTLPRRFSSLALDQFGVMPNHLHGILILPDDVGRGESGIR
                     PLKEGGRTKQGEHKVRPYGTLPRTVGRIVQAFKSTATGEYIKGVKNLGWPTFPGRLWQ
                     RDYYEHVIRNEESLNKIRQYILQNPLKWMCDSENPATESDAAVLEKSQRAEKDFWEEL
                     KRPLR"
     gene            complement(1518..2840)
                     /locus_tag="AC16FU_10490"
     CDS             complement(1518..2840)
                     /locus_tag="AC16FU_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR2994835.1"
                     /translation="MKAFKRMGYLTLGFLFFGAPFQVAAGAQLSMTLEDCLRLAQEKN
                     YNLRSAAQQVELTQAKKEEAQAGFFPTLTLTGNYTYLGREPRAQIPVIPPLSPVARPI
                     TIGFHDNWDFKMGLSYTVFSWGRVGNSVAAADYAWQAAQKDAERNWHSVRLDVTRTFW
                     SVLLAQELVRVRSEAQKSLKRHQEQVAARLTAGQASRFDLLRAKVEEANALPPLLAAQ
                     HQVESGLDLLKNLLGLPPESDVTLPGELAEESLSVDLSTAVATALQKRPELQALALQK
                     EMARKNRATAGAGLRPNLLGSAAFDYKNPFNAQKVWKTDWNFSAAIVFPLFDGLATRA
                     RIQQAEVAAKQVSIADEQLRQTIVLEVREAVRAIEEAQKNSATQRENVKLAKESLEIA
                     KVQYQHGLVTNLEALDAELAVLVAQSNYLSALFNFKVAKAQYEQAVGW"
     gene            3051..3239
                     /locus_tag="AC16FU_10495"
     CDS             3051..3239
                     /locus_tag="AC16FU_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR2994836.1"
                     /translation="MRKRFLFAPAFVLTLGLYPAAFSQVQVGQQAPDFTLTDVTGVPR
                     TLSQYRGQVVFLNFFGWG"
BASE COUNT          665 a         1035 c          866 g          770 t
ORIGIN      
        1 gaattcatcc agtccacccc ggagaggtcg atcaccacac tctttttgtc gctcttcacc
       61 aggtcgtgca gttgctcatg caaaagcgtc gcctccggcc cgcccataat cttccccatg
      121 ggcgaaagga tcgtcacgcc gcgctcctcc cgcgtggcca gtttcatgac cacgctccgc
      181 gcttactcca atccttcata cgccggtgct ccgccagggt ttcatactac cgctgcttca
      241 cttgaagatt gccttgattt tcccccaggt cgtcagtttg accgccgccg cggccacggt
      301 caggtctttc tgttgcgcac cggcaaaggc ctcaaaactg gggtaggcga ggttgccgtt
      361 caccgagatg aagaccagat cgcccgactc ccagccctcc ttctgcggcg tttgggaatc
      421 gtccttggca atcaaaagta cgtagttgcc gccctgggta tagccccgtg cataaacgac
      481 accgttaatc gtggccacga cggcagtgct gtccggcgcc ggcccgccgt ggatcgttac
      541 cgtgccgtac ggctggcagc tggaggggtt gggcggatcc tgatcctgcg ccagcgtccg
      601 tgcgctcagg cagactgcaa cgaccacaat tactggcaac gtttttgacg cctgtcccat
      661 agcccaataa gccgtgagga gtcgcttctt ccactgaggc gaataaaacc aacccatcgc
      721 cccggtaagt aatctgggcc gccccgcaat gtcaagtgaa attcctttct ggttctgtag
      781 cggtttgatt aatcaaaccc cttccgaccc ctataaatac cgccgctaag gtggcctgcc
      841 gggttgcccc taccgcagcg gtcgctttaa ttcttcccaa aaatcctttt ctgccctctg
      901 gcttttctcc aaaaccgccg catccgattc tgtggcggga ttctctgaat cgcacatcca
      961 cttcaaggga ttctgcagaa tgtattgccg gattttgttt aaggattctt cgttccgtat
     1021 gacgtgctcg tagtaatccc gctgccacaa cctgccgggg aatgtcggcc aaccgaggtt
     1081 tttgacgcct tttatgtatt cgccggttgc agtggatttg aacgcctgaa cgatgcggcc
     1141 cacggtccta ggcaatgttc cgtaggggcg aaccttgtgt tcgccctgtt tagtccggcc
     1201 accttcctta agagggcgaa taccagattc gcccctaccg acgtcatcgg gcaaaatcaa
     1261 aatcccgtgc aaatggttgg gcataactcc aaattgatcc agcgcgaggg aagaaaaacg
     1321 gcggggtagg gtccgccaga ttctctcgac cattcgcccg gcatcgttca attgcatttt
     1381 cccgtccgtc acagaaccga aaaaacattc ccgcgcctgg gtacagatcg tcacaaaata
     1441 tgccccggcc cgggaataat cgtactcggc cagccggatt gtccggcggc gtggttgcgg
     1501 cttctgatcc ccgaccttca ccaccccacc gcctgctcat actgcgcctt cgcaacctta
     1561 aaattaaaca acgcagacag gtaattgctc tgcgccacca gcacggccaa ttcggcgtcc
     1621 agcgcttcca aattggtgac cagcccgtgc tggtactgca ccttcgcgat ttccagactt
     1681 tctttcgcta atttgacatt ttctcgctgg gtggcgctgt tcttttgggc ttcttctatt
     1741 gcccgcaccg cctcccgcac ctccaacaca attgtttgtc gtagctgctc atctgcaatt
     1801 gacacctgct tggccgccac ctccgcctgt tgtattcgag cgcgggtggc cagaccgtcg
     1861 aagaggggga aaacgatagc ggcagaaaaa ttccaatccg tcttccagac cttctgtgca
     1921 ttgaaggggt ttttgtagtc gaaggcagct gacccgagga gattgggccg caaccccgcg
     1981 ccggccgtcg cccggttctt ccgggccatc tctttttgca acgccagcgc ctgcagttcg
     2041 ggccgcttct gcagtgccgt agcgaccgcc gtcgaaagat ctaccgagag ggattcctcc
     2101 gctaattcac ccgggagcgt aacgtcagat tcgggcggca gcccgagcag atttttcaaa
     2161 aggtcaagcc ccgactccac ctgatgctgt gccgccagaa gcggcggaag ggcattggcc
     2221 tcttcgactt tcgcccgcaa aaggtcgaag cgcgacgcct gccccgccgt caaccgcgcc
     2281 gccacctgct cctgatgccg tttgagactt ttttgcgctt cgctgcggac ccgcactaat
     2341 tcctgcgcca gaagcaccga ccagaatgtc cgggttacgt ccaggcgaac gctgtgccag
     2401 ttacgctccg catccttttg ggcggcttgc caggcgtagt ctgccgccgc aaccgagttt
     2461 ccgaccctcc cccaggagaa aacggtgtaa ctcagcccca ttttgaaatc ccagttgtcg
     2521 tggaacccaa tggtgatggg ccgcgccacc ggagacaggg gcgggattac cggaatctgc
     2581 gctctcggct cccgccccaa gtaagtgtag ttgccggtga gggtcaaagt cgggaaaaac
     2641 ccggcctggg cctcctcttt tttcgcctgc gtcagttcaa cctgctgtgc ggcgctgcgc
     2701 aaattgtaat tcttttcttg cgccaaccgc aggcagtctt ccagggtcat tgagagttgg
     2761 gctcccgctg ctacctggaa aggtgcgcca aaaaacaaaa aacccaacgt caggtacccc
     2821 attcgcttga acgctttcat ccgtcctcct ttagtcgctt ccgccggaga ataatacagg
     2881 atgagcgccg cagatgtaca actgcaaaat ggagggagaa cgagtgaacg gatttcaaat
     2941 ccgcgtcagg tccaaagaaa atgacggaaa ttgtatgcct attgcgaact tttcttgtcc
     3001 ggaatatgtt tagattagta cagggctttc gcccgaacca aggagcaggt atgcgcaaac
     3061 gctttctctt cgccccggcc ttcgttctaa cgctcggcct ttatccggcg gccttttcgc
     3121 aggtccaggt cggacagcag gcacccgact ttaccctgac cgacgttacc ggtgtccccc
     3181 gcaccttaag ccagtaccgg ggccaagtgg tctttctgaa ctttttcggc tgggggtgac
     3241 cgtcttgctt ggcggtggca tcgtcaatcg aacagaacat ttggcaggta tatcagaatc
     3301 aaggcgttca ggtcttgggc attgacgtct ggaacg
//
LOCUS       DBNGZA010000494         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2011411, whole genome shotgun sequence.
ACCESSION   DBNGZA010000494 DBNGZA010000000
VERSION     DBNGZA010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2011411"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1373)
                     /locus_tag="AC16FU_10500"
     CDS             complement(312..1373)
                     /locus_tag="AC16FU_10500"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HIR2994837.1"
                     /translation="MSKAKSLFEHLAHKPARWLSGDGPEAGIVLSSRVRLARNIAGLT
                     YPTVADSDTRRQVVDALRQAVEKSVLLKSGELITSGEITELDRNFLIERHLISPEFMK
                     EKDHHALYVGEDEALAIMVNEEDHLRIQAITSGLEIARTYELADQADVELAGTLAFDF
                     DRDFGFLTACPTNVGTGMRASVLIHLPGLVLTRGIDAVINRITKVGLAVRGFYGEGSD
                     VLGNLFQISNQTTLGRSEQDILDSLEKVTRQIIEYELSARETLVRDAKEQIEDKIWRA
                     YGILKYARVLSSAEVMNLLSAVRLGLGLGFIRDVSLGTINEILLLSQPSHLQKYHQKE
                     LSSNERDILRAELVRSKLA"
     gene            complement(1370..>1850)
                     /locus_tag="AC16FU_10505"
     CDS             complement(1370..>1850)
                     /locus_tag="AC16FU_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR2994838.1"
                     /translation="ASFHITQIINNQQAMLNLCHECAEKRGIHNPIQGMPFPLGELLA
                     SMTSTQPAGEKKRGKTADPKCPGCGMSFSEFGKTGRFGCGQCYSAFRPQLTDLLRKIH
                     GSNQHQGKSPSAKSEKLQPLREERKLQDELRRAIASEDFEKAALLWDRLRTLTGKEL"
BASE COUNT          389 a          520 c          537 g          404 t
ORIGIN      
        1 tcctcgcagt aactttgaga agaaagaagc ggaacgcaga caaaatcctt acggacacag
       61 cacctccagg tgggtagaac gacgaaggca acctatccac taacaaggta caatgagagc
      121 gcaaaacttg tcaagacatt ttccacttat tgacgcaaaa aatccgcatt gtgcttctga
      181 gaacgatgat ggtattatta ttgagaaatt gagaataatc cgtttgtccg gcaatgagtt
      241 agcaaacgaa tttgggaaaa cgttcacaaa caagagcaga ctcgtgaagt gcctgctccg
      301 caaaatctgc gttatgccag tttggaacgg accaattcgg cgcgcaggat gtcgcgttcg
      361 ttggaggaaa gttctttttg gtgatacttc tgcaggtgcg acggctggct caacaacagg
      421 atttcattga tcgtccccag cgacacgtcg cggatgaacc ccagacccaa acccagtcgt
      481 accgcggaaa ggaggttcat cacttcggcg gaactgagca cccgggcgta cttcagaatg
      541 ccgtaggccc gccagatttt gtcctcaatt tgctctttgg cgtcacgcac cagcgtttcc
      601 cgcgccgaca gttcgtattc gatgatctgc cgcgtcacct tctccaagct gtcgaggatg
      661 tcctgttcgg aacggcccag ggtggtctgg ttggaaatct gaaagaggtt ccccaggacg
      721 tcgctccctt cgccgtagaa gccgcgcacc gccagcccga ccttggtgat gcggttgatg
      781 accgcgtcga tgccgcgggt aagcaccaag ccgggcaggt gaatgagcac cgaagcccgc
      841 atgccggtgc cgacgttcgt ggggcaggcg gtgaggaaac cgaaatcccg gtcaaagtca
      901 aacgccaaag tgccggccaa ctcgacatcg gcctgatcgg ccagctcgta ggtgcgggcg
      961 atctccaggc ccgaggtgat ggcttgaatc cgcaggtggt cttcttcgtt gaccatgatc
     1021 gccagcgctt catcctctcc gacgtagagc gcgtggtggt ctttctcctt cataaattcc
     1081 ggcgaaatga gatgcctttc gatcaagaaa ttacggtcca gttcggtgat ttctcccgag
     1141 gtgatgagct ctccgctttt caagaggacg cttttctcca ccgcctggcg cagggcgtcc
     1201 accacctgcc ggcgcgtgtc gctgtcggcc acggtggggt aggttaaacc cgctatgttg
     1261 cgcgccagcc gcacccggct ggaaaggacg atgccggctt ccggtccgtc gccggagagc
     1321 cagcgggccg gtttgtgcgc cagatgctca aacaaagact tcgccttgct cacaattcct
     1381 tccccgtcag ggtgcgcagc cggtcccaca ggagggcggc tttttcaaaa tcctcggagg
     1441 caatggcccg gcgcagttcg tcctgcaact tgcgctcctc ccgcaagggc tggagtttct
     1501 cgcttttggc cgacggcgat tttccctgat gctgattgga accgtggatc ttgcgcaaga
     1561 ggtcggtcaa ctgggggcgg aaggcgctgt agcactggcc gcagccgaaa cgcccggttt
     1621 tgccaaattc agaaaagctc attccgcaac ccggacactt gggatcggcg gtttttcccc
     1681 gcttcttctc tccggccggt tgagtggaag tcatgctggc caagagttcg cccaagggga
     1741 agggcatacc ctggatggga ttgtggatcc cgcgcttctc ggcgcactcg tggcacaagt
     1801 tgagcatcgc ctgctggttg ttgatgatct gggtgatgtg gaaactggct
//
LOCUS       DBNGZA010000495         2697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1480599, whole genome shotgun sequence.
ACCESSION   DBNGZA010000495 DBNGZA010000000
VERSION     DBNGZA010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1480599"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2697
                     /locus_tag="AC16FU_10510"
     CDS             <1..>2697
                     /locus_tag="AC16FU_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR2994839.1"
                     /translation="SDARSQFFNTSSSLEGNRWTDNGNGTYTTNTVKDYFMRLDQYLM
                     GLRGSGTVDSLWFIRNPSNGSFACTRAADFTTTVSGQKVWVDINDIIGAEGPRNPDVS
                     TSQKNFRMAFILVVQNGIPPTPYELARLDSARYQWMSYFRTGVENLGTMNTDLPAPGP
                     LEIVSDTLTKGAVGGTYRDQVFAVGGIPDYPFYALMSGSLPPGLALNTSTGVISGTPT
                     TAGGYNFTVQVCDSSSTCVSKQLHIDIYATGTGTVVINEVELFSRGGSVELFNKGSQA
                     KNIGGWKVVTKTLSASDSMIVPANTIIPPGCYYVLTEYAGTNTANWFYFNVTVPWTNG
                     SSGSCALKDNLGTGVDFCRWGSSAEPVPAGTTWSGGNPAAPSGSRNLGRDGNSTDTNN
                     SSDFTSQVGTLGRQNLPYTTQRQTATTSTPTIQALLTNWNNVASQGSFPTLSYISDGL
                     SFLYDASFFAGLVKLSGDTAVYRSIFSDLLFSLAGPLTVDAVSDPRATHARSASPTID
                     GLISVDAHYILPKTADSGQFLICQYTVHNLSTDTLSNVLLGMAADYDVPASTSANTSG
                     FDSTKNLVWLQSTDTPSRFAGMAGLAVNRVYAAHALDNPTYVYPANGYVTSQLYQVAN
                     TPGFSTAASQPNDLNVVLTQQKTTLPPGECATASFALILSRTNQADLLASADKAKAFL
                     GNLRARYVATTGNDVTGTGTAGNPFATIQKGIDQAQDGDTVIVQPGTYAGGINVNKPV
                     HLKSALRDSATISGGSPYAVTLSSGCVLDGFIVRSEAVGNILVQSAAGIRPEIEHNQL
                     HSAPLGIQVTGGANGALIHHNLISRTDIGVLLANTTGTTEIFHNTFVANDTGILVSQA
                     ANGNIINNIIDSSGIGLSAAGGCPAHSYNDFSNISGSTYVGCV"
BASE COUNT          555 a          847 c          755 g          540 t
ORIGIN      
        1 ctctgacgcc cgctcgcagt tcttcaacac ctcctcctcc ctggaaggaa accgctggac
       61 ggacaacggg aacgggactt acaccaccaa cacggtgaaa gactatttca tgcggttaga
      121 ccagtacctg atggggctgc ggggctcggg cacggtcgat tcgctctggt tcatccggaa
      181 tccctcgaac gggagtttcg cctgcacccg cgcggccgat tttaccacga cggtgagcgg
      241 gcagaaggtg tgggtggaca tcaatgacat catcggggcg gaagggcccc ggaatccgga
      301 tgtctccacc tcgcaaaaaa acttccgcat ggcttttatc ttagtggttc agaacggcat
      361 cccgccgacg ccatacgaac tggcccggct ggacagcgcg cggtatcagt ggatgagtta
      421 cttccgcacc ggcgtggaaa acttagggac gatgaacacc gatttgccgg cgcccgggcc
      481 attggaaatt gtttccgaca ccctgaccaa aggggcggtg ggcggcacct atcgggatca
      541 ggtcttcgcg gtgggcggaa tcccggacta ccctttctac gctcttatga gcggcagttt
      601 gccgccgggg ttggcgttga atacttcgac cggcgtcatt tccggcacgc cgaccacggc
      661 cggggggtac aatttcaccg tgcaggtgtg cgattcgagc agcacctgcg tttccaagca
      721 actccacatc gatatctatg cgaccggcac cggaacggtg gtaatcaatg aagtggaact
      781 cttttcgcgc gggggctcgg ttgaactctt caacaaaggt tcccaggcta agaacattgg
      841 cgggtggaaa gtggtaacga aaacactttc tgcttcagac tcgatgattg tacccgccaa
      901 caccatcatt ccgcccgggt gctactacgt tttgaccgaa tacgccggaa ccaacacggc
      961 caactggttc tacttcaacg tgaccgttcc ctggacgaac gggtcatccg gttcgtgcgc
     1021 cctgaaggac aacctgggca ctggcgttga cttctgccgc tgggggagtt cggctgagcc
     1081 ggtgcctgcg ggaacgactt ggagcggagg taatccggcg gcgccttccg gtagccggaa
     1141 cttgggccgg gatggcaact ccaccgacac gaacaacagt tccgacttca cctcgcaggt
     1201 cggcacgtta gggcgccaga atctgcccta caccacccag cgccagacgg caacgaccag
     1261 cacgccgacg attcaggcgc ttttgaccaa ctggaacaac gtcgcctcgc aggggagctt
     1321 tcccacctta agctacatca gcgacggtct gtcctttctc tacgacgcct cgtttttcgc
     1381 cgggctggtg aaactctccg gcgacaccgc cgtctaccgc agcattttct ccgacctctt
     1441 gttctccctg gccggtccgc tcactgtgga tgcggtttcc gacccgcggg cgacccacgc
     1501 gcggtcagct tcgcccacca tcgacggact cataagcgtc gatgcccact acatcctgcc
     1561 caaaacggcc gacagcgggc agttcctcat ttgccagtac acggtgcaca acctctccac
     1621 cgatacgctc tcgaacgtgc tcttggggat ggctgccgat tacgatgtcc ccgcgagcac
     1681 gtcggccaat accagcgggt ttgattccac caaaaactta gtctggcttc agtccaccga
     1741 cacgccttcc cgttttgccg ggatggcggg actggcagtc aatcgtgttt acgctgcgca
     1801 cgcactcgac aacccaacct atgtttatcc cgctaacggg tacgtcacca gccaactcta
     1861 ccaggtggcg aacacccccg gcttttcaac cgccgccagc cagccgaatg atttgaacgt
     1921 cgttctcacc cagcagaaaa ccaccctccc gcccggcgaa tgcgccacgg ccagttttgc
     1981 cttgattctc tctcgcacca accaggccga tcttttggct tcagccgata aagccaaggc
     2041 attcttaggg aacctgcggg cccgctacgt ggccaccacc ggtaatgacg tcaccggtac
     2101 gggcaccgcg ggaaatcctt ttgcgacgat ccaaaaggga attgaccagg cccaggacgg
     2161 cgatacggtc attgtccagc cgggcaccta tgccggcgga atcaacgtga acaagccggt
     2221 gcacctgaag agtgcgctgc gtgattcggc cactatcagc ggcgggtcgc cctacgcggt
     2281 gacgctctcc agcgggtgtg tgctggacgg attcatagtt cgcagcgaag ccgtgggaaa
     2341 tatcctggtg caaagtgccg ccgggatccg tcccgaaatt gagcacaacc agctccacag
     2401 cgcgcctttg ggcattcagg taacgggcgg agcgaacggc gcccttattc accacaatct
     2461 catcagccgg accgacattg gtgttcttct ggcgaatacg acggggacca ccgagatctt
     2521 tcacaatact ttcgtggcga acgacaccgg tattctggtc agccaagctg cgaacgggaa
     2581 catcatcaac aacatcatcg attcgagcgg gattggtctt tcggcggctg gcgggtgtcc
     2641 ggcgcactcc tacaacgatt tctcgaacat ttcggggagc acgtacgtcg gttgtgt
//
LOCUS       DBNGZA010000496         8303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1373156, whole genome shotgun sequence.
ACCESSION   DBNGZA010000496 DBNGZA010000000
VERSION     DBNGZA010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1373156"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC16FU_10515"
     CDS             <1..290
                     /locus_tag="AC16FU_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994840.1"
                     /translation="LPELTGSDAVRQERPHLAGQPALQVTFQKPYTVQIPLTTNPNYF
                     ETVQEFQMGTVTLVKRDGVIYLIYFICDRQFFRINLEQVAGMLASWQFTGP"
     gene            380..1501
                     /locus_tag="AC16FU_10520"
     CDS             380..1501
                     /locus_tag="AC16FU_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR2994841.1"
                     /translation="MKKQSWFWAWSGAGVLVVSGVLLSSCVEKVPPPLGAAPILSGNF
                     VTLKPENLDSLPSSQVYELWLLRSTPHGTEQKSLGKFRWDKFLYAARHPDGSRITENK
                     FDAGEDVFAWAEIAITIEQANDPSPAPSASVFFLGTINDTLPDLRLTYPINLTKEEFK
                     GDFFLASPTDSAGFEFLYTDFTTPPLADRQRRESQGIWFGDLALAKEAKRDTAQFVGV
                     TPGLVLPKLSAGWQYEGWVLVGGKYLSTGKFLRADSADFTNPYLGPKATGKQTFTSGD
                     SLRFTTDFKIPGEDFILNPPSGFVFPLSMIRTPASGDSVFVTIEPSPDPSPATPFRSL
                     TLYGAQLPTALSSSFIHQNLGLANISRRAPAVAVSISDK"
     gene            complement(1537..2745)
                     /locus_tag="AC16FU_10525"
     CDS             complement(1537..2745)
                     /locus_tag="AC16FU_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994842.1"
                     /translation="MTKGIFLWMSFLFWATAATPVQSQPGRTAYVVNGLAETLGKINL
                     ENGAVVESIATLGVVPNQIVLRGRRGYVVNSISSNVQIINLATGVTEGTVELGPSRNP
                     WNLALADTETAYVTNFAAGTLSRINVVTRQVLGEFPVGLSPEGLAVVDGLVLVCNTGF
                     NPVDFSYGQGTVAVFDPAVDSVVKVIPVGSNPQAIAQAPSGVLHVVCTGNYVNISGKI
                     YEIDPATLTVVDSILIGGQPGAIGIGPNGIAYVAAGGFVGSGIVFSYDTFTGTVLHGR
                     GNPIATGTGASAVAVDNLNQALVCDFQAATVTQLDSLGNPLATYALGDGPLSVAIYDT
                     TRLGDLDGDGLVDLADIVTAVQHVVFDRRPLVIRLADVNADCVVDLLDIIVLVRHIVF
                     GTPQLLPGCA"
     gene            complement(2763..4964)
                     /locus_tag="AC16FU_10530"
     CDS             complement(2763..4964)
                     /locus_tag="AC16FU_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2994843.1"
                     /translation="MQLGFVCGVVFFSLSPEPITGQSTDKLQGFVRDSQSSAPVAGAV
                     VELVGTNYRDITDERGFYRFEHLPVGTYSVVVRSIGYRTRRIEQVGVVQDVTARLDIT
                     LEAKPIPLPPQEVKGLGPASKFSSTQDVTIISHKEIERSGAGSLAELFQTVPGLFVYQ
                     NGSASGEKALSVRGSAANQVVILVNGQRLASGATGTADLAGIPLAIVERVEVVKGGQA
                     ARIGPDALAGVINIITRTVSGKRTVVEVTGRVGSFRTQEGEVTFGRQLTERWQVTGSA
                     WAQATAGDFPYRDSLGQVFLRENAAREAANFYASASANLFRGGNLQFSLQWYETGNGI
                     PGALLQFTPAATRREQRTLFSAAWNQPVTDHFQMELAGSLQRYRQVFQNSETPDLFSR
                     FWADTRTQLDQISGRWNWSVRRGGVLSFGSEYLSGQFTSTDLLRPHLSLGKAPRRETI
                     GFFLAGEAKLEHPWPPLVDLAVLSAAVRRDKTSGFSGATNPHVGLLLARGDAWKITAK
                     GNWQRAYRNPALVDLFWKEDALAVGNPQLKPERSSDWDAGGEVRLPLLGQVTVGDTWF
                     GSDVRDLVVWQRRFDGKYTPQNLIRAQISGREESFGWESRDEKIKAGLQHTYSRAVDR
                     SGFRPHEGKQLVFRPRHVLRAWGQFTWRGWSLASSYRWVDRRFIREANTKSLPPYHLI
                     DLTCGVTFPRKAGWEIRSAFKVTNLTNQAYELLERQPMPGREWRFELRFKK"
     regulatory      complement(5176..5384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5453..6181)
                     /locus_tag="AC16FU_10535"
     CDS             complement(5453..6181)
                     /locus_tag="AC16FU_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994844.1"
                     /translation="MSTSSSPEPRPPARPLHRKAGWPRRLWRILAAPRPGRVFVYFLV
                     FYLALYPLYQVINQPYTAFVVKITSSVFHVVTTRHAVPSVSMDGNGIKVKVLITPRGG
                     LPDPTAKVFTGLRTAYDLGNYIVFYLALLLATSFQASRRENLKAFLGGLVWLLAAHVF
                     DLSLYATFGYHEAAAVLAESRQYAYHSTLHRIVSFLLDAEVAIFPRAVPLVLWIFFFR
                     KRLGAMARMWRERRLLRRTGKTPV"
     gene            complement(6159..6728)
                     /gene="xrtH"
                     /locus_tag="AC16FU_10540"
     CDS             complement(6159..6728)
                     /gene="xrtH"
                     /locus_tag="AC16FU_10540"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HIR2994845.1"
                     /translation="MSKRSRQEKNLPPHPSKRSITGRLIYFIREHKKLFRMYLFFGLA
                     LLALFGVVTLLDKTPFPGWLNRRLAHASGTVLRLIDPPVTVAGEYIGMGNFGAAIAEE
                     CNGIYATVILLAGFLAFPAPWIKKFIGVALGIVALQIINVVRIVVLFLIGKHFPSFFE
                     ASHLYVAEFVVIACGAGLWLLWYVYIVKP"
     gene            complement(6925..7356)
                     /locus_tag="AC16FU_10545"
     CDS             complement(6925..7356)
                     /locus_tag="AC16FU_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994846.1"
                     /translation="MVSPVLAAGDSVRVTITTGSHTIAVTSITFTINSSPVSVTPVPP
                     STGPGQVVSRTYAVAAQPTACTMPFLMDGIGHSFVGNPLTYATDLTLPFHGSPDSKVR
                     FTFISVLPTLTQWGLVIFGMLLAGTLTSIYLLRRRKARLAT"
     gene            complement(7959..>8303)
                     /locus_tag="AC16FU_10550"
     CDS             complement(7959..>8303)
                     /locus_tag="AC16FU_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994847.1"
                     /translation="EGQASDAFGFPLTIGLAKPPLPVQFSLSQNRPNPFNPQTTIEFS
                     LPLSSPVRLVIYNVAGQVVRTLLESDLPAGVHAVVWDGRNDQGIEVSSGIYFYQMAAG
                     SFQETKKMVLLK"
BASE COUNT         1841 a         2487 c         2325 g         1650 t
ORIGIN      
        1 tgctccccga actcaccggc agcgatgctg tccgtcagga acggccccat ctggcgggtc
       61 aaccggccct ccaggtgacc ttccagaaac cgtataccgt gcaaatcccc ttgaccacca
      121 atccgaacta tttcgaaaca gtccaggagt ttcaaatggg gaccgtgacc ttggtcaagc
      181 gggacggggt gatctacctg atttatttca tctgcgaccg ccagttcttc cggatcaatc
      241 tggaacaggt agccgggatg ctggcctcct ggcagttcac cggcccgtga ggcagggaat
      301 caccgcttga catttgacga gaggggctgt attgtagttc ccggtagatg ggtacttcag
      361 gagcgatgcg ggagtgacta tgaaaaagca gagttggttt tgggcgtgga gcggtgcagg
      421 ggtgctcgtt gtgagcgggg tattactttc atcctgcgtc gagaaagttc cgccgccctt
      481 gggggcggcg ccgattctct ccggcaactt cgtcacgttg aagccggaga atttagattc
      541 gctaccttcc agccaggtct atgagctctg gcttctccgt tccacaccgc acgggaccga
      601 gcaaaaaagt ctcggcaaat tccgctggga caaattcctc tacgctgccc ggcatccgga
      661 cggctcacgg attaccgaaa ataagtttga tgccggcgag gacgtttttg cctgggcaga
      721 gatcgccatc accatcgagc aggccaatga ccccagtccg gcgccctcgg ccagcgtttt
      781 ctttttgggg acgatcaacg acaccctgcc cgacctgcgc ctgacctacc ccatcaatct
      841 cacgaaggaa gaattcaagg gcgatttctt cttggccagc cccaccgact cagccggttt
      901 tgaattcctc tataccgatt ttaccacccc gcccctggct gaccgtcagc gccgtgaatc
      961 acaggggatc tggtttggtg acttagccct cgccaaggaa gccaaacggg acacggcgca
     1021 attcgtcggc gtcacacccg gtttagtgct gccgaaactc tccgcgggtt ggcagtacga
     1081 aggctgggtg ctcgtcggcg gaaaatacct gtccacgggg aaatttctcc gtgctgactc
     1141 agccgacttc acgaatccgt acctcgggcc gaaagccacg ggaaagcaga ccttcaccag
     1201 tggggattcg ctgaggttca caaccgattt caaaattccc ggtgaagatt tcatcctcaa
     1261 ccctccgtcg gggttcgtgt tccccctctc catgattcgc acccctgcct ccggggattc
     1321 ggtttttgtc accatcgagc cgtctccaga tcccagtccg gccacaccct tccgctcttt
     1381 gacgctttat ggcgcgcaac tcccgaccgc tctcagcagt tccttcatcc atcaaaatct
     1441 tggattggcg aatatttccc gccgcgcgcc ggcggtcgca gtgagtatca gcgacaagta
     1501 atcgccgcac tccacacgga cggacgctct gccaggtcag gcgcacccgg gcaaaagctg
     1561 gggcgtcccg aagactatgt gccggaccaa cacaataatg tccagcagat caaccacgca
     1621 gtcggcattc acgtccgcca gacgaataac cagaggccgg cgatcaaaca ccacgtgctg
     1681 gacggcggta acgatatcgg caagatccac cagcccatcg ccgtccagat cacccaaccg
     1741 cgtcgtatcg tagatcgcca ccgaaagcgg cccgtcaccc aaggcgtaag tcgccagcgg
     1801 gttgcctaga gaatccaact gggtaaccgt cgccgcctga aagtcgcaca ccagcgcctg
     1861 gttcaggttg tccaccgcca ccgcactggc gccggttccc gtggcgatcg gattcccgcg
     1921 tccgtggagc acagtgccgg tgaaggtatc gtaactaaag acgataccgc tgccgacaaa
     1981 accgccggcc gcgacgtacg caatcccgtt cggtccaata ccgatcgccc cgggctgccc
     2041 gccgatgagg atgctatcca caacagtcag cgtcgctgga tcaatctcat aaattttgcc
     2101 ggatatgttc acgtagtttc ccgtgcaaac gacgtggagc actcccgaag gagcttgagc
     2161 gattgcctgc ggattgctgc cgaccggaat caccttgacc accgaatcga ccgccggatc
     2221 gaacaccgcc accgtcccct ggccgtagga aaagtcgacc ggattgaacc cggtgttgca
     2281 gaccaagacc agaccgtcaa cgacggccaa tccttcgggc gatagaccca caggaaattc
     2341 acccaacacc tgtcgcgtca cgacattaat ccgggaaagc gtgccggcgg cgaagttcgt
     2401 gacgtaggcg gtttcggtat cggccaaggc gaggttccag ggattgcggc tggggcccag
     2461 ttccaccgtc ccctcggtga ccccggtagc gagattgatg atctgcacgt tggaagaaat
     2521 cgaattgacg acgtagccgc gccttccgcg caggacgatc tggttgggca cgacccccag
     2581 agtcgcgata ctctcgacga cggcaccgtt ttccaggttg attttcccca acgtttccgc
     2641 tagtccgttc accacgtaag ccgtgcgacc cggctgagac tggaccggtg tggccgccgt
     2701 cgcccaaaat agaaaactca tccagagaaa tattcccttc gtcattatcc ctcccttttg
     2761 gtctactttt taaacctgag ttcaaatcgc cactcccggc ccggcatcgg ctgccgctct
     2821 aagagttcat aagcttgatt ggtgaggttg gtgactttga acgcggagcg gatttcccac
     2881 cccgccttcc gcgggaaagt gacaccgcag gtgaggtcaa tcaagtggta aggcggcaga
     2941 gacttcgtgt tagcttcgcg aatgaagcgg cggtccaccc agcggtacga cgaggccagc
     3001 gaccaccccc gccaggtgaa ctgtccccat gctcgcagaa cgtgccgggg ccggaagacc
     3061 aattgcttcc cttcgtgcgg gcggaacccg gagcggtcaa cggcgcggct gtacgtgtgt
     3121 tggagacctg ccttgatctt ctcatcgcga ctctcccagc cgaacgactc ctcccgcccg
     3181 ctgatctggg ctcggatgag gttctgcggc gtgtacttcc cgtcgaaccg cctttgccag
     3241 acgacgagat cgcggacgtc actgccgaac caggtgtctc ccacggtgac ctgccccaga
     3301 agcggcaacc gcacttcgcc gccggcatcc cagtcggagg accgctccgg cttcagctga
     3361 ggattcccca cggccagggc gtcttctttc cagaacaggt cgaccagagc cggattgcgg
     3421 taggcccgct gccagttccc tttggcggtg attttccagg catcacctct ggcaagcaaa
     3481 agccccacgt ggggattcgt agcgccgctg aaaccactgg tcttatcccg ccgtactgcc
     3541 gcggaaagca cggccagatc aacaaggggc ggccagggat gctcaagctt tgcttcaccg
     3601 gcaagaaaaa atccgattgt ttcccggcgg ggtgcttttc ccaaactcaa gtgcggtcgc
     3661 aagagatcgg tgctggtaaa ctgcccggag agatattcac tgccaaagga gagcaccccg
     3721 ccccgccgca cactccagtt ccagcgtcct gagatttggt ccagctgggt gcgagtgtct
     3781 gcccaaaagc gggaaaaaag atccggcgtt tcactgtttt ggaaaacctg acggtagcgc
     3841 tggagggaac cggccaattc catctggaaa tgatcagtga ccggttggtt ccacgcggca
     3901 ctaaaaagag tgcgctgctc ccgccgtgtc gctgctggag tgaactggag cagtgcgccg
     3961 gggatgccgt tccccgtttc gtaccactgc agggaaaact gcagattccc cccccggaaa
     4021 aggttggcgc tggcactggc atagaaattg gccgcctccc gggccgcatt ctcccgcagg
     4081 aaaacctgtc cgagcgagtc gcggtacgga aaatcaccgg cggtggcctg cgcccaggcg
     4141 ctcccggtga cctgccaccg ctctgtcagc tgccggccga acgtgacttc tccctcctgg
     4201 gtgcgaaaac tccccacgcg tcctgtgact tcgacaacgg tgcgtttacc ggatactgtt
     4261 cgggtgataa tgtttatgac acccgccaga gcgtcgggcc ctatgcgcgc ggcctggccc
     4321 cctttcacca cttccacccg ttccactatc gccaaaggaa ttccggcgag atcggccgtt
     4381 cctgtcgcac cggatgcgag cctttggccg ttcaccagaa ttaccacttg atttgcagcg
     4441 ctgccgcgga cggaaagagc cttctctccg ctggccgatc cgttctgata gacgaaaaga
     4501 ccgggaactg tctggaagag ctccgccaag gacccggcac cggagcgctc aatttccttg
     4561 tgcgagataa tcgttacgtc ttgcgtggag gaaaattttg aagcaggacc cagacctttg
     4621 acttcctgcg gtggaagagg gatgggtttt gcttccagcg tgatgtcgag ccgggcggta
     4681 acgtcctgga cgacgcccac ctgctcgata cgacgcgtgc ggtagccgat tgaccgcacc
     4741 acgactgaat aagtacccac cggcaggtgc tcgaaccgat aaaatccacg ctcatcggtg
     4801 atgtcccggt agttcgtgcc aacaagttct acgaccgcac ccgctacggg cgcgctgctt
     4861 tgactgtcac ggacaaaacc ctggagcttg tcggtcgatt gtcctgtaat cggttccggt
     4921 gacagggaga aaaacaccac tccgcagaca aaaccgagct gcaccagcca cccgcggaaa
     4981 acagagttac agcaacgatg gtgccgcgct gagacaggct ttctgaaccg caaaatttct
     5041 ccctatgaat aaaaaccccg gccgcctcac cttcgtcggc cgggggacag cgcataaata
     5101 tatccgcacc ttacccgcga aggtcacggc ggtcgcttcc tctctgattg gattggttca
     5161 gagaggcact gcttgaaatc tggctggttt cctgacttga cggcttcggt cctaccggcc
     5221 gcgccttccc attccgcctc aggcgggaca gtggctggtg cggctttcgt agccgtttac
     5281 agtagcgggg ctgtggccgc tttgcacggc cttcccactt agtcctcgta cgagtccata
     5341 cggatccgta gggatcgtac cgacccatac gggaacacca gattccgctt tgattctatg
     5401 ctgcgccggt taatctgtca aggcttttcc tgcagggggg caaaagagag tttcaaaccg
     5461 gtgtttttcc tgtccgtcgc agcaaccgcc gctcgcgcca catccgggcc atcgccccca
     5521 accgtttccg aaagaaaaaa atccagagca ctagcggcac cgcgcggggg aagattgcga
     5581 cttcggcatc caaaagaaat gagacgatcc ggtgcagcgt agaatggtag gcatactgcc
     5641 tggattccgc caagactgcc gccgcttcgt ggtagccaaa cgtggcgtaa agtgaaaggt
     5701 caaaaacgtg tgccgccaga agccagacca gaccgccgag aaaggccttg aggttttcgc
     5761 gccgggaggc ctggaaactc gtagccaaaa gcaaagccag atagaagacg atgtagttcc
     5821 ccagatcgta ggcggtgcgc aaaccggtaa acaccttagc ggtgggatcg ggcaacccgc
     5881 cgcggggcgt aatcaacact ttgaccttga taccgttgcc atccattgag actgacggta
     5941 ccgcgtgccg cgtcgtgacc acgtgaaata cggaactggt tattttgaca acaaaagcgg
     6001 tatagggttg gttgattacc tggtagagcg ggtagagcgc caggtagaaa accagaaaat
     6061 agacgaatac ccggccgggc ctcggcgcgg ccaggatccg ccatagacgg cgcggccatc
     6121 ccgccttgcg gtggagcggc cgggccggcg ggcgcggttc agggcttgac gatgtagaca
     6181 taccataaga gccacaaccc cgcgccgcag gcaatgacga caaactcggc gacgtagagg
     6241 tggctggcct caaagaaact gggaaagtgt ttgccaatca aaaacaaaac gacgatgcgc
     6301 accacgttga taatctgcaa tgcaacgatc cccaatgcca cgccgatgaa cttttttatc
     6361 cagggtgccg ggaaagccag aaatcccgcc aaaagaatca ccgtggcgta gatgccgttg
     6421 cactcttcgg caatggcggc gccgaagttg cccattccga tgtactcgcc agcgacggtc
     6481 accggcgggt caattagccg gagcaccgta cccgatgcgt gcgccagcct ccggttcaac
     6541 cagcccggaa aaggggtttt gtccagaagc gtaacgacgc cgaagagggc cagaagcgcc
     6601 aggccaaaaa agaggtacat ccggaacagt tttttgtgtt cacggatgaa atagatcagg
     6661 cgtccggtga ttgatctctt tgaaggatgt ggagggagat ttttctcctg ccgtgatctt
     6721 ttgctcatta tggctctgcg cgattgactc cgtctaaatc tacccttagg gaaagcggtc
     6781 tgtcaacgtc caatacccga cggtcagtgg gcacgaaccg tgagggggag actgtcgcta
     6841 ctggcgcagc aactatttga agtaagcgaa aagggccggt cagcagatct gaccagccct
     6901 gcttttccta tgcccaataa cggcttacgt ggccaggcgg gctttccggc gccggagaag
     6961 atagatggat gtgagagtcc ctgccagaag cattccaaag ataaccagcc cccattgggt
     7021 gagagttggc agcacgctga tgaaggtgaa gcggactttg gagtcgggcg agccgtgaaa
     7081 cggcagcgta aggtccgtag cgtaggtcag cggattgccg acgaaagaat ggccgatgcc
     7141 atccatcaga aatggcatgg tgcaggcggt gggctgggcc gctaccgcat aagtccgcga
     7201 gacgacctgc cccggccctg tggatggcgg caccggtgtc accgaaaccg ggctgctgtt
     7261 aatggtaaag gtgattgagg tcacggctat cgtgtgcgac ccggttgtga tcgtcacccg
     7321 cacgctgtca ccagcggcta aaacgggact taccataagt ccgacaaaca caagcgtgag
     7381 tactgcagtt accagtctgc gcatgctacc tccgatgatt gaaaaccgtt tacttttcct
     7441 gcccattggg cctcaaacca tttgaggggg tgcctgccca aagccttccc cgtgcgacca
     7501 accataacct ccctgactcc tgtggtcaag aaccgttccg ctctgagaaa agttcccgtt
     7561 aatatataga aattgaaaaa gtcccgcttt agggcgggac ttcttctctt gcgcgcctcg
     7621 caataccccc gaagagggag tctgagggtg caggcgcgta tcttagccgt tcaccggaac
     7681 tatagtaaca aacggcgtgc cggacagcac ggttgcgtaa aaacttactt aactcgttac
     7741 tgcaccggtg gtttcggaaa taaaatctct tcctcaaagg gaaggataag cgcctgtttc
     7801 gcaattcctg cgtgctgtga atcccgacct ctaaagagaa tcctcttaat ccagcatatg
     7861 ccaacgctat gaaatgaaac gcactttctg cgccccaaat tcgtatttac agcttttccc
     7921 cacggagaca cggagaacac agagaaattt ttcagaaatt acttcaagag caccatcttc
     7981 ttagtctcct ggaaagaacc cgccgccatc tggtagaagt agatgccgga agaaacttca
     8041 atcccctggt cattgcggcc atcccagacg accgcgtgaa ctcctgccgg caaatcgctt
     8101 tccaaaagtg tccgcaccac ctgacccgcg acattgtaaa tcaccagccg gaccggagag
     8161 gaaaggggga gagagaattc aatcgtggtc tggggattaa atgggttggg ccggttctgg
     8221 gaaagtgaga attgaacggg caaaggcggc ttggccaggc caatcgtcag cgggaacccg
     8281 aaagcgtcgg aagcctggcc ctc
//
LOCUS       DBNGZA010000497         6184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_630136, whole genome shotgun sequence.
ACCESSION   DBNGZA010000497 DBNGZA010000000
VERSION     DBNGZA010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_630136"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="AC16FU_10555"
     CDS             <1..1131
                     /locus_tag="AC16FU_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR2994848.1"
                     /translation="LIADAIRKGASDLHLETYEKRIRVRYRIDGTLREMAPLPFKYRA
                     AIVSRVKIMADLDISERRLPQDGRIKVKITERTVDLRVSVLPTIFGEKVVMRILDPKS
                     LMVDLTRLGFHEHSLGHFQKAIELPYGIILVTGPTGSGKTTTLYSALKQINTNDINIM
                     TAEDPVEFNFDGVNQVLVRSDIGLTFSAALRSFLRQDPDVIMVGEMRDQETAEIAIRA
                     ALTGHLVFSTLHTNDAPSTVNRLVDMGIPAYLVASATKLIMAQRMVRKICSVCKADDP
                     DVSPQALERLGVPPNEIKAFKAYKGKGCNECGGTGYSGRSGLFEVMPVTPAIEHLILN
                     NATASDIRKVAVEEGMLTLRMAGIEKLKQGLTSLEEVLVETA"
     gene            complement(1228..1695)
                     /locus_tag="AC16FU_10560"
     CDS             complement(1228..1695)
                     /locus_tag="AC16FU_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994849.1"
                     /translation="MRKIVVVFLTIVIWTGWLIASGQAQMSSTTFRIPTQALSSSGGA
                     GNSTNFKQPVSIGGQSTPIGTATSTNFDNFAGYLYTLGGGACALLGDLTGDLVVDIVD
                     IVAMVNNVVFGDPIPTGAICADIAPIPLDGVIDITDIICLINNVVFSNPLPCP"
     gene            2150..5077
                     /locus_tag="AC16FU_10565"
     CDS             2150..5077
                     /locus_tag="AC16FU_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR2994850.1"
                     /translation="MRRFARISLAPLGFCLVFSVFSVSVWAGVTGKIAGVTRDKETKK
                     PIPAATVQLVGTAVGAFSNPDGEYFLINIPVGTYKIKATSVGYAPVEVDNVHVTADQT
                     TTLDLELEPQAVQMQEIRVTAERPLIQRDQTGTSRVVTSQEFANLPVRDFKDAAALQT
                     GVVKGLISPPYLDRSRGDDISSGLYVRGGRSNEIGTYVDGFTQQNPLTGLSTVTINPN
                     AIEEIQVLTGGFSAEYGRIMSGVVNTVTRSGTAKYHGSFGAVTDNLAGRWIDTKSFDN
                     NVYEASFGGPFKPNDDRYTFYFSGEQRWNRDQSPGRVSSVAQQTIDDIKRTQGFSEGD
                     IRSEQGRLPANHLKGWSAQGKLSFKLTPNMNLQAGALLTQDDFRQYRQEFLFDLDHTP
                     RVINTNRSVFVKLNHNLNRKTYYNAAVNWFFTDRFRGDGLYGDNLKEYGRTYGNPNFN
                     INGPLGTIFWPGDDPSTPIVRNAEGIITKDTLGRNTDESSLFDDFLKQRSWYIGGAFN
                     VASQVTEHHLVQGGADFQRHTLRRYHHLYPANVGLDNKATRYVDADYFGYDSAFTKEV
                     NGGDNGAKHPLLFSVYLQDKMETEQAIVNLGLRYDYLSSQTKLLADPSRPFLGAPSVY
                     TCQGCLVANKARHRVSPRLGVAFPIAERTQFHINYGKFFQLPNLEDLYVGLPYLNYKV
                     PLAGYYFAFGNPNLLPEHTTAYEVGVTQGLTENSRVDVTAYFKDVSGLVEVANIESNP
                     SNFASFRNNDFGTIKGVDISYKTRRTRALSLEVNYSYSQATGTGSTSQTQRNIAWTAT
                     EPPKLTHPLDFDQRHKISMIADLRAEKGQGPKVGNYHILENAGINVLLNAGSGLPYTP
                     AKPFDAVTLAAVSLQPSGPINSSYRPWTFRIDLKATKKFSYAGADLEFYVTILNLTDR
                     KNALTVYPTSGSPTSTGFLSTETGAATYDTPAKQALYHLAEQNPSSFDVPRMVRFGTR
                     LSF"
     gene            5117..>6184
                     /locus_tag="AC16FU_10570"
     CDS             5117..>6184
                     /locus_tag="AC16FU_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994851.1"
                     /translation="MKIGRRALGIGGLWLLAAVGVWAAPPSQKATPPLAKPGAPQIPD
                     NSQVIDINNLLMWVTNHGSFAWDIQSGDAGLQFPKPAPGQPPKTVVFASGLWVGGKVR
                     DTIRVALGEYSQEYVPGPMAQPCTPAGLDGPGCFLPDNANFRVFKVLRNNPTALDWPN
                     WIIAARQGAPFVGTDSNTPAVLGDQMLWAVYNDASPAIHTNTAGSSKPLGIEVQQTTF
                     AFNRTGALGQTIFLKFKVINKGQNTIDSCYLSLWADPDLGGFTDDLVGCDTTLSLGFC
                     YNATNTDEQYGSTPPSVGYDFFQGPIGDLGQRLPMTSFNKYINGTDPASKDETYNYMK
                     GLQPDGSPLINEVTGDTTTFFV"
BASE COUNT         1377 a         1834 c         1721 g         1252 t
ORIGIN      
        1 ctgatcgccg acgccatccg caaaggcgcc tccgatcttc acctcgagac ttatgaaaaa
       61 cggatccggg tccgctaccg gatcgacggt accctccgcg agatggcacc tctgcccttc
      121 aagtatcggg cggccattgt ctcgcgcgtc aagattatgg ccgatttaga catttccgag
      181 cgccgcctgc cccaggacgg ccggattaag gtcaagatca ccgagcgcac cgttgacctc
      241 cgggtatcgg tcctgcccac cattttcggc gaaaaagtgg tgatgcgtat cctggacccc
      301 aagagcttga tggtcgactt gacccgattg ggtttccacg aacactccct gggacatttc
      361 cagaaagcga tcgaactccc ctacggcatc atcttggtca ccggaccaac cggttccgga
      421 aaaaccacca ccctttattc ggccttgaag cagattaata ccaatgacat caacatcatg
      481 accgctgaag atccggtgga gttcaacttc gatggggtca accaggtgct ggtgcggagt
      541 gatatcgggc tgaccttttc ggcagcccta cgctcgtttt tgcggcagga cccggacgtc
      601 attatggtgg gtgaaatgcg tgaccaggaa accgccgaga tcgccatccg ggcggccctc
      661 accgggcatc tggttttctc cacgctccac accaacgatg caccctcgac ggtgaatcgt
      721 ctggttgata tggggattcc ggcttacctg gtcgcctcgg cgaccaaatt gattatggcc
      781 cagcggatgg tgcgcaagat ctgttcggtc tgcaaggcgg atgacccgga tgtgtcgccc
      841 caagctctgg aacggctggg ggtaccgccg aatgagataa aagcgtttaa ggcctataag
      901 ggaaaagggt gcaacgagtg cggcggaacc ggctactccg gccggtcggg tctttttgaa
      961 gtgatgccgg tcacgccggc cattgaacac ttgatactca ataacgccac ggcttccgat
     1021 atccgcaaag tcgccgtcga agaaggaatg ctcaccctgc ggatggccgg gatcgagaaa
     1081 ctcaagcagg gcctcacctc tctcgaagaa gtgttagtcg agacggcgta gtatttcgac
     1141 ttcacttctc ccaaaaaaga aaggcacccc tacggtcgga gcgactcgag gcagggtgct
     1201 tttctttccg taccttgatc ctctccctta cggacagggt aaggggttgc taaagacgac
     1261 gttgttgatc aggcagatga tgtcggttat atcgataacg ccgtcgagag gaatgggggc
     1321 gatatcggca cagattgcgc cagtggggat gggatcgccg aagacgacgt tgttgaccat
     1381 ggcgacaatg tccacaatgt ccaccaccag atcaccggtc aagtcaccca agagggcaca
     1441 ggcacctcca cccaaagtgt acaagtagcc ggcgaagttg tcgaagttgg tgctcgtggc
     1501 agtgccaatc ggcgtgctct ggccgccgat ggagaccggc tgcttgaagt tggtcgaatt
     1561 ccccgcaccg ccggaggaac tcaaggcctg cgtgggaatc ctgaaggtgg tgctggacat
     1621 ctgggcctgg ccgcttgcga tcaaccaacc ggtccagatg acgatagtca ggaaaacgac
     1681 cacgatcttt cgcatggctg ctcctttcag actccttacc gttttcgcgt tcatacgcat
     1741 aaactgtcgt gcgccctccc tcgggggata agaaaagggc agggccaact cctgtcaagg
     1801 gattttaagg ctgagggcta gatttttttc aaaatatgca aattttactt gacaaatgaa
     1861 ccggtgtact gttcctatat ccccggaagt taagaagttg cagtgactgt gggccgttta
     1921 cggctcgttt tgagaaacga accaaaagga ggagaatcgg tccaacgcga cagagtatag
     1981 cctgacaact gcatatctgc cgtcgcgcgg tgaaggaatt ggcccacgtg ccgatgttcc
     2041 tcatagatag aggagtatgg gagtagtggg attccaggat ggatgtcgtg gtacccggcc
     2101 gccaggtcgg gagaagttta ctttcctttc caaccaaaag ggaggagtta tgcgcaggtt
     2161 cgcgcggatc agtctggctc cgctggggtt ctgtctggtg ttttccgttt tttcggtttc
     2221 tgtttgggcg ggagtgaccg ggaagatcgc cggagtgacg agagataagg agacgaaaaa
     2281 gccgattccc gcggcgacgg tccagctcgt cgggaccgcg gtgggcgcct tctcgaaccc
     2341 cgacggggag tattttctga tcaacattcc ggtggggacc tacaagatca aggcgacgtc
     2401 ggtcgggtat gcccctgtag aagtggacaa tgttcacgtt accgccgacc agaccaccac
     2461 gctcgatctg gaactggagc cgcaggccgt gcagatgcag gagatccgag tgacggccga
     2521 acggccttta atccagcgcg accagaccgg gacttcccgg gtggtcacgt cgcaagaatt
     2581 tgcgaacctc cccgtgcgcg actttaagga tgccgccgcg ctgcagacgg gcgtggtgaa
     2641 gggtctcatc agccccccct atctcgatcg gagccgtggc gacgatatca gctcgggttt
     2701 gtacgtccgc ggcggccgct cgaacgaaat cggcacttac gtggatggct tcacccagca
     2761 gaatccactt accgggcttt cgacggtgac gatcaacccg aatgccattg aggaaattca
     2821 ggttttgacc ggcggttttt ccgccgagta cggccggatt atgtccgggg tggtcaacac
     2881 cgtgacgcgg agtggaacgg ccaaatacca cggttctttc ggagcggtaa ccgacaactt
     2941 agctggccgc tggatcgaca ccaagagttt tgacaataac gtttacgaag cttcattcgg
     3001 cgggccgttc aagcccaacg acgaccggta cactttctat ttctccgggg aacaacgctg
     3061 gaaccgtgac cagtcgcccg gacgcgtcag cagcgtcgcc cagcaaacga ttgacgacat
     3121 caagcggacc caggggtttt ccgaaggcga cattcgttcc gagcagggcc gcctgcccgc
     3181 caatcacctg aagggctgga gcgcccaggg gaaactgagt ttcaaactca ccccgaacat
     3241 gaaccttcag gcgggggcac ttctgaccca ggacgacttc cggcagtatc ggcaggaatt
     3301 tctcttcgac ctggaccaca cgccccgggt catcaacacc aacaggtcgg tcttcgtgaa
     3361 gttgaaccac aacctcaacc gcaagaccta ctacaatgcc gccgtcaact ggttctttac
     3421 cgacaggttc cgcggcgacg gcctgtacgg cgacaacctg aaagaatacg gccgcaccta
     3481 cgggaacccc aacttcaaca taaacgggcc gttaggaacc atattctggc ccggagatga
     3541 ccccagtact ccgatcgtgc gcaacgccga aggcatcatc accaaggata ctctgggtcg
     3601 aaacacggat gaatcgtcgc tctttgacga tttcctgaag cagcgttcct ggtatatagg
     3661 cggtgcgttc aacgtcgcct cgcaggtaac cgagcaccac ctggtgcaag gcggcgctga
     3721 tttccagcgc cacaccctgc ggcgctacca ccacctctac cctgccaacg tgggtctgga
     3781 caataaagca acccgctacg tggatgccga ctactttggg tacgactcgg ccttcaccaa
     3841 agaggtaaat ggcggcgata acggcgccaa acatcccctg ctcttctcag tctacctgca
     3901 ggacaaaatg gaaaccgagc aggccatcgt aaatctcggc ttgcgctacg actacctctc
     3961 gtctcaaacg aagctactgg cggatccttc ccgaccgttc ctcggcgcac ccagcgtcta
     4021 cacctgccag ggttgcctgg ttgctaacaa agcgcggcac cgggtaagtc cccggctggg
     4081 ggttgcgttt cccatagcgg aacggacaca gttccacatc aattacggga agttcttcca
     4141 gttgccgaac ttagaggacc tctacgtcgg cttaccctat ctgaattaca aggttcctct
     4201 ggccggttac tacttcgcct ttgggaaccc caacctgtta ccggagcaca cgactgcgta
     4261 cgaggtgggt gtcactcagg gattgacgga gaactccagg gtcgatgtga ccgcctactt
     4321 caaagatgtc tccggtctgg tggaagtggc caacatcgag tccaatcctt caaacttcgc
     4381 ctcgttccgc aacaacgact tcgggaccat caaaggagtg gacatctcat ataagacacg
     4441 gcggacccgg gcactgtctc tggaagtaaa ctactcatac tctcaagcca ccggcaccgg
     4501 ttccacctct cagacccaga ggaacattgc ctggaccgcc accgagccgc cgaaactgac
     4561 ccaccccctc gatttcgacc agcggcacaa aatttccatg attgccgacc tgcgggcgga
     4621 aaaagggcag gggccgaagg tgggcaacta ccacatcctg gagaatgccg gcatcaacgt
     4681 gcttttgaat gccggctcgg ggttgcccta cactccggcc aagccgttcg atgcggtgac
     4741 cttggcggcg gtgtcgctcc aaccatccgg gccgatcaac tccagttacc ggccctggac
     4801 tttccggatc gacctcaaag ccactaagaa attttcctac gccggggcgg atctggagtt
     4861 ctacgtgacg attctgaacc tgaccgatcg gaaaaacgcg ctcaccgtct acccgaccag
     4921 cggctcacct acatcgactg gttttctctc caccgaaacc ggagccgcaa cctacgacac
     4981 gccggccaaa caggcgctct accacctggc agaacagaac ccctcgagct ttgacgtgcc
     5041 gcggatggtg cgttttggca cccgcttgtc attctaaccg gagagaaaca gcagtttgac
     5101 cttgaggagg gaacctatga aaatcggaag acgcgccttg ggcataggcg gcctttggct
     5161 tctggccgcg gtgggggtct gggcggcacc gccaagtcaa aaagcaacac cacctctggc
     5221 caagcccgga gcgccgcaaa tccccgacaa cagccaagtg attgacatca acaacctttt
     5281 gatgtgggtc accaatcacg gctcgtttgc ctgggacatt caatcgggtg atgccgggct
     5341 acagtttcca aagccagctc ccgggcaacc acccaagact gtggtgtttg cctccgggct
     5401 ctgggtcggg ggaaaagtgc gcgatacgat ccgggtggcc ctgggtgagt attcgcagga
     5461 gtacgtcccc gggccgatgg cccagccctg cactcctgcg gggctggatg gccccggctg
     5521 ctttctgccg gacaacgcca atttccgggt cttcaaggtg ctgcgcaaca accccacagc
     5581 cctggattgg ccgaactgga tcatcgctgc ccgacaggga gctccctttg tgggcaccga
     5641 cagcaacact cccgcagttc tcggagacca gatgctttgg gcggtgtaca atgacgcctc
     5701 tccggccatt cacaccaaca ccgctggatc gtctaagccg ttgggcatcg aagtgcagca
     5761 gacgactttt gccttcaacc gcaccggtgc cttggggcag acaatttttt tgaagttcaa
     5821 ggtgatcaac aaggggcaga ataccatcga ctcctgctac ctctccctct gggccgatcc
     5881 cgatctgggc ggctttaccg acgatctggt gggctgcgac accactttgt ctctgggttt
     5941 ctgctacaac gccactaaca cggacgaaca gtacggttca acgccgcctt cggtcggcta
     6001 cgacttcttc caaggcccca tcggagattt aggccagcgc ctgccgatga cctccttcaa
     6061 caagtacatc aacggtaccg acccggcttc caaagacgaa acctacaact acatgaaagg
     6121 gttgcagccg gatggaagtc cgctcatcaa cgaggtgacc ggcgatacga cgaccttttt
     6181 cgtt
//
LOCUS       DBNGZA010000498         3993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_419047, whole genome shotgun sequence.
ACCESSION   DBNGZA010000498 DBNGZA010000000
VERSION     DBNGZA010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_419047"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1735
                     /gene="accC"
                     /locus_tag="AC16FU_10575"
     CDS             320..1735
                     /gene="accC"
                     /locus_tag="AC16FU_10575"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR2994852.1"
                     /translation="MFKKVLIANRGEIALRVIRACNELGIATVAVHSEADANSLHVRF
                     ADEDVCIGPGLAKDSYLDPKRIISVAEVTNADAIHPGYGFLAENADFAEICQACNLVF
                     IGPTPQQIRLMGDKLAAKNTMAKAGVRVIPGSDGPVNTLEEALEVAEEIDYPLMIKAA
                     AGGGGRGLRCAMDPSDLKSMFDTARAEAQAAFGNPAVYLEKFLPNPRHVEIQILGDHH
                     GNVIHFGERDCTIQRRHQKLLEESPCPILPERRRHQMAEAAVRGAKNIKYRGAGTVEF
                     LYDPTGHFYFMEMNTRIQVEHPVTEEVADIDLVKEQIKVAAGEKLSLRQGDVKLSGHA
                     LECRINAEDPERDFMPCPGELSAFHIPGGHGIRVDTHAYVKYVIPPFYDSLVAKVIAH
                     GKTREEAIIRMRRALEEFTIEGIKTTIPFHLKVLHHERFLAGQYDTAFVEELQRELGV
                     ERVTAVSEPAAPVPEGVQASA"
     gene            1931..2311
                     /gene="gcvH"
                     /locus_tag="AC16FU_10580"
     CDS             1931..2311
                     /gene="gcvH"
                     /locus_tag="AC16FU_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR2994853.1"
                     /translation="MVIKPELKYTKEHEWVKVDGAIATVGITDYAQGELGDVVFVELP
                     KVGTAVEQMKPFGTIEAVKAVSELYAPLSGEVAEVNSRIEKEAGIINKDPYGEGWLIK
                     IKVKNSQELAALLDASRYEALIKT"
     gene            2529..3932
                     /gene="gcvPA"
                     /locus_tag="AC16FU_10585"
     CDS             2529..3932
                     /gene="gcvPA"
                     /locus_tag="AC16FU_10585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR2994854.1"
                     /translation="MTYFPHTPNDRRKMLERIGVGRFEDLLRELPPELVLQKPLALPK
                     RLSELETAAHLRSLAGENGSSQEYVSFLGGGIYDHYVPAAVDAILRRPEFYTAYTPYQ
                     AEVAQGTLQAIYEFQSLICQLTGMEVSNASMYDGPSAVAEAALLATGQTGRKEILFPA
                     TLNPHYQKVLATYSRGGAMKLAMVRGAEGTIDLEDLKKKITDRTAAVIVQQPNFYGCL
                     EKPAEFEPLVHQAGALLIAVCDPISLGLLAPPGEYNSDLAVGEGQSLGIPTQLGGPGF
                     GFFAGKKELVRRMPGRIAAETVDAKGRRGYVLTLQTREQHIRREKATSNICTNQALCA
                     LAAAVYLSLLGRAGIKDIAELCLQKAHYAVDQLARLPGFRLRFASPFFKEFVLETPVP
                     ARKIIRAALKKGIFAGVDLGQYQRELKNCLLIAVTEKRTRAEIERLVDLLAKVTQARQ
                     TSIPGPERQSAPQKVTV"
BASE COUNT          919 a         1134 c         1193 g          747 t
ORIGIN      
        1 caacccggac gacttcgacc tgcgggtcaa gggcatcacc ggcgccgccg accggggctg
       61 gacggagttt gaacagagcc agaagccgcg ggtgtgaggg ctttgcagag agattactat
      121 ctcggacccg cgtaggcggc ccgtacgggc tgcctttttc ttaacaccac accgccaaaa
      181 acgggttagc ctccatagcc gaggcttcca gcctcggcgt gcgtaaacac aatttctatc
      241 tcatatattc aaaaatgcat ctggttgacg gcggttgaag tattatattg cgcctcaatg
      301 tggaagggga gaagcggaga tgtttaagaa agttctaatc gccaaccggg gggagattgc
      361 gctgcgggtt atccgcgcct gcaacgagtt agggatcgcc accgtggcgg tgcactccga
      421 ggcggacgcc aattcgctcc acgtccggtt cgccgacgaa gacgtctgca tcggccccgg
      481 gctggcgaag gattcgtact tagatcccaa gcggatcatc agcgtcgcgg aggtgaccaa
      541 cgccgacgcg attcatcccg gctacggttt tttggccgag aacgccgact tcgccgaaat
      601 ctgtcaggcc tgcaacctgg ttttcatcgg ccccacaccc cagcaaatcc gcttgatggg
      661 cgacaaactt gccgccaaaa acaccatggc caaagccgga gtgcgggtca ttccggggag
      721 tgacgggccg gtgaacacgc tcgaagaagc gctggaggtg gcggaggaaa tcgactaccc
      781 gttgatgatc aaagcggcgg cgggcggcgg tgggcggggc ctgcgctgcg caatggatcc
      841 gtccgatctg aaatcgatgt ttgacaccgc ccgggccgaa gcgcaggccg ccttcgggaa
      901 cccggcagtc tatctcgaaa aatttctccc gaatccgcgc cacgtcgaga ttcaaatctt
      961 aggcgatcac cacgggaacg tcatccactt cggggagcgc gactgcacga ttcaaaggcg
     1021 gcaccagaaa cttctggagg agtcgccctg tccgatcctg ccggaaaggc ggaggcacca
     1081 gatggctgag gcggcggtcc gcggcgcgaa aaacatcaag taccggggcg ccggaacggt
     1141 tgaattcctc tacgacccga ccggacattt ctatttcatg gaaatgaata cccgaattca
     1201 ggtggagcac ccggtgaccg aagaggtggc cgacatcgac ttagtcaagg agcaaatcaa
     1261 agttgctgcg ggagagaagc tctccctgcg ccagggggac gtcaaactct ccggccacgc
     1321 cctggagtgc cgcatcaatg ccgaagatcc ggagcgcgat tttatgccct gccccgggga
     1381 actctccgcc tttcacattc ccggcgggca cggcatccgg gtcgataccc acgcctacgt
     1441 caagtacgtc atcccgccct tctacgattc gctggtggcc aaagtgatcg cccacggcaa
     1501 aacgcgggag gaagcgatca tccggatgcg gcgggcgctg gaggaattca ccatcgaagg
     1561 aatcaaaacg acgattccgt ttcacctgaa agtgctccac cacgagcgct ttttggccgg
     1621 gcagtacgat accgcctttg tggaagaact gcagcgggaa ttaggagtcg agcgggtgac
     1681 cgccgtttct gaacccgccg ctcccgtacc cgagggcgtt caggcatcgg cgtaaagtga
     1741 caggtaaagg atgacctcac cccatacggg ccgtatggcc taccctctcc taaggagagg
     1801 gatcaaaaaa gatgacctca cctatctcct ctgaagagcg ggtaaagtca ggaaaattcc
     1861 ctccccttta ggggagggcc ggggtggggt cagaagtaga cgtttactaa agatgaaagg
     1921 aatcgagcga atggttatca agcccgaact gaagtacacc aaagaacacg agtgggtcaa
     1981 agtcgatggc gctattgcca cggtgggtat caccgactac gcgcagggag agttaggcga
     2041 cgtggtgttc gtggaactcc ccaaagtcgg gaccgccgtc gaacagatga aaccgttcgg
     2101 aaccatcgaa gcggtcaaag cggtttcgga actgtacgcg cctctgtccg gcgaagtggc
     2161 cgaggtgaac tcccggatcg aaaaagaagc cgggatcatc aacaaggacc cctacggtga
     2221 ggggtggctc ataaaaataa aggtcaaaaa cagtcaagaa ttagccgcgc tcctggacgc
     2281 cagccgctac gaggcgctga ttaagacgta gcttttctcg tttgcttgcc tctcaacttc
     2341 gcagggtatc ttcggaggtt ttccctcccc ttgagggtcg atagactcgt accgaggagg
     2401 gtgagtgagg ggtcggcaga gaggtttact taggcttggc acactctccg caacggacta
     2461 tattgcgtcc tgttacgtat tctgaatagg gaaatgattc gttccatgac cgaaagacag
     2521 gcaaacgcgt gacttacttt ccgcatacac ccaacgatcg ccggaagatg ctggaacgca
     2581 tcggcgtggg ccgctttgag gacctcctgc gcgaacttcc gccggaactt gttttacaaa
     2641 aaccgctcgc tttgcccaaa agactctccg aactggaaac ggcggctcat ttgcggtcgc
     2701 tggccggaga gaatggttcc agccaggagt acgtttcgtt cctgggcggg gggatctacg
     2761 accactatgt ccccgccgcg gtcgacgcca ttctccgccg cccggaattc tataccgcct
     2821 acacacccta ccaggccgaa gtggcccagg ggacgttgca agccatttac gaatttcagt
     2881 cgctcatctg ccagttgacc gggatggagg tttccaacgc ctcgatgtac gacggccctt
     2941 cggctgtggc cgaggcggcg ctcctggcga ccggacaaac cggacgcaaa gaaattcttt
     3001 ttcccgccac cttgaatccg cactatcaga aagtgctggc gacctactcg cgcggcggag
     3061 ctatgaaatt ggcgatggtc cgcggcgcgg aaggcacaat cgatctcgaa gatctcaaga
     3121 aaaaaataac cgaccgcacc gcggcggtaa ttgtccagca gcccaacttc tacggttgct
     3181 tagaaaagcc cgccgaattc gagccgctcg tccaccaggc gggggcgcta ttgattgcgg
     3241 tctgcgatcc gatttcgctc ggcttgctcg cgccgcccgg cgagtacaac tccgatctgg
     3301 cggtggggga agggcagagc ctgggcattc ccacccagtt gggcgggccg ggttttggct
     3361 ttttcgccgg caaaaaagaa ctggtgcgcc ggatgccggg caggatcgcc gcggaaacgg
     3421 tcgatgccaa aggccggcgc gggtacgtcc tcaccctgca aaccagggaa cagcatatcc
     3481 ggcgcgagaa ggccacctcc aacatctgca ccaaccaggc gctctgcgcg ctggcagccg
     3541 cagtttacct ttcactcttg gggagagcgg gcatcaagga cattgccgaa ctctgcctgc
     3601 agaaggcgca ctatgctgtg gatcaattgg ccagacttcc gggcttccgg ctgagatttg
     3661 cgtcaccttt tttcaaggaa ttcgtcctcg aaacccctgt gccggcgagg aaaatcattc
     3721 gtgccgcgct gaaaaagggg atttttgcgg gagtcgatct gggccagtac cagcgagaac
     3781 tgaaaaattg cctattgatt gcggtgaccg agaaaaggac gcgggcggag attgagcgcc
     3841 tggttgacct cctggcaaaa gttacgcaag ccaggcagac ctcaattccc ggtcccgaac
     3901 ggcagtccgc accgcaaaaa gtgactgtat gagaacgagc ggacgatgag aatgaccaaa
     3961 cagaacgaaa ggaggagtga tgaggcgaag tct
//
LOCUS       DBNGZA010000499        11631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2328166, whole genome shotgun sequence.
ACCESSION   DBNGZA010000499 DBNGZA010000000
VERSION     DBNGZA010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2328166"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1078
                     /locus_tag="AC16FU_10590"
     CDS             230..1078
                     /locus_tag="AC16FU_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR2994855.1"
                     /translation="MALAQIAPKLGDVQANLKTHLALIRKARARKADLIIFPELSLSG
                     YLLKDMVPRVALKLDAPEVKQLLTESKSIAMAVGLVEETPESLYYNSALFLEKGKMVY
                     CHRKVYLPTYGMFDEGRFFGAGEALRAFDTRAGRLGVLICEEAWHSFCPYLLTLDGAV
                     AVINMANSTARGLDGKRRVGTAQTWELMNRFYAGMHQVFFIFVNRVGFEDGIGFWGGS
                     EVIDPFGKVAAKAPYFREQLVFADIDLGQVEQARLRTPLLRDERLDFSLAELARVRDQ
                     RQTTLA"
     gene            1203..2048
                     /locus_tag="AC16FU_10595"
     CDS             1203..2048
                     /locus_tag="AC16FU_10595"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR2994856.1"
                     /translation="MSLSNRDTPTLLNIDPKVTTEILTAFLVNEAGKFGFTKAVVGVS
                     GGLDSATVAILAARAYGPNNVLGVLLPYKHSSPDSLKDGLALVRQTKIRHEVVDITPL
                     AEPYIERFKAIDRVRIGNVLARLRMLVLFDLSHRGASLVMGTSNKTELLLGYSTWYGD
                     MASSLNPIGDLYKSQVRQLAAFLKVSKPIQKKIPTADLWVGQSDEGELGFTYDEVDRL
                     LYYLIDELATREQLVSLGFRESLVKRVERMIYNSQFKRSLPIICKLSRRTVGIDFQLS
                     KDWGR"
     gene            2069..2764
                     /gene="rsmI"
                     /locus_tag="AC16FU_10600"
     CDS             2069..2764
                     /gene="rsmI"
                     /locus_tag="AC16FU_10600"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR2994857.1"
                     /translation="MLYLVATPIGNLEDISYRALEVLKRVDLIAAEDTRHTGLLLKHF
                     GIQARMVSYHEHNEARRAAELLELLKSGKSIAIVSDAGTPGISDPAYRLVQSAIQDQI
                     PVVPIPGATAFVPALVASGLPTDRFVFEGFLPVKSGRRRKRLEELRTEERTIIFYESP
                     YRLVRLLGELQEVLGDRKAVVAREVTKKFEEFVRGPLSEIRAHFQLHPPKGEMVVLVA
                     GTKNAVEPRSEAE"
     gene            2761..3720
                     /locus_tag="AC16FU_10605"
     CDS             2761..3720
                     /locus_tag="AC16FU_10605"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR2994858.1"
                     /translation="MNRVKPHNGWFFLPVDWAILIYLGSISLAMLFFRENVPHWPLYL
                     AFNLAVSGLVFSLARQLGNPQRGLARFFRHWYPIFLFTFFYEETGPLVHIFFPNWFDM
                     HVVHLERVLTGGAPSVWLEQFASRPLSEFLMFGYFSYYLLLPVVGLILWLKRREQEFD
                     RMIFIAAVTFFVCYWGFYLYPVQGPRWAIGDWHQVPIRGWIFVPVVNFFINLAVRGGA
                     MPSSHVAVALVVLLFAWRNVRWLGIILTPIVAGLAVGTVYGRFHYAADVVVGIAIGWG
                     CDFVLNRWLARRAVREKLPAFVPGRMAPARPAFAERRSGAAPE"
     gene            3722..4537
                     /gene="lgt"
                     /locus_tag="AC16FU_10610"
     CDS             3722..4537
                     /gene="lgt"
                     /locus_tag="AC16FU_10610"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR2994859.1"
                     /translation="MHPEIIKLGPFALRSFGLFLALSFFAGLGLIKWRAKKYGTDFNR
                     LVNLAFVVIISGILGARLFYVFSHWSEFAGHPLDIINPFQSGQVVGIAGLTLYGGVIL
                     AIVCGSIYLKAARLPFWPTFDIFAPAIAFGIFLTRIGCFLNGCCFGMPTALPWGVHFP
                     ADSIPAYYFPGATLHPAQLYASAYGLLLFLVLVPLDNRKKFYGSTFAWFLIGESIFRF
                     LLEFVRYADPGTFFTLDGSRWTYNQLVAVGLFAGGVAMRIFLRKSFPLTVQKA"
     gene            4683..5381
                     /locus_tag="AC16FU_10615"
     CDS             4683..5381
                     /locus_tag="AC16FU_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR2994860.1"
                     /translation="MDKVQPTAAAGELGSDDQVISFKENLKRGAHAALDPLVRALARL
                     GMRADHLTILGLVFGLGAGVLFFAGRFRLGALLAALAGLCDLLDGQVVRLTKSVSRFG
                     AFLDSTLDRLAEAAILVGIAGFYIAHPEVLATGPEPGSWAVAVSLFAMLALVGSFLVS
                     YTRARAEGLGIECKVGWFERTERMVLIIIAGLFGVGPVMPGALVVLVLLSFATAAQRV
                     AYVWKKTHGAGLDR"
     gene            5396..6499
                     /locus_tag="AC16FU_10620"
     CDS             5396..6499
                     /locus_tag="AC16FU_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR2994861.1"
                     /translation="MSKVRVAIIGVGNCASSFVQGVEYYKNAKDGDFVPGLMHVNLGG
                     YHIRDIEFSAAIDIDKNKVGKDLSEAIYTKPNNTYKFCKVPNLGVKLVRGMTHDGLGL
                     YLSKIIEKAPGPTADIVKTLKDTGTDVVVNYLPVGSEEATKWYVEQVLDAGCGFVNCI
                     PVFIAREKYWQKRFRDRGLPVIGDDIKSQVGATILHRVLARLFRDRGVKLERTSQLNV
                     GGNTDFLNMLERSRLESKKISKTNAVTSQLDYNLGEENVHIGPSDYVAWLTDRKWAYI
                     RLEGRTFGDVPLNIELKLEVWDSPNSAGVVMDAVRCCKLAMDHKLSGAIEAPSAYFKK
                     SPPVQYTDEEARRMTEEFIQKYGRKAKPARVKK"
     gene            7366..8163
                     /locus_tag="AC16FU_10625"
     CDS             7366..8163
                     /locus_tag="AC16FU_10625"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR2994862.1"
                     /translation="MPTAQILTEREQKKYLALDQEAKKLLEFKKENIRKNHLTPCPQC
                     ATWINFKAKKCPQCTSDIAGHMQKVREQLKRLDEISAELYELHKKKMELYHEGSAYKP
                     IWERVQAFFSDQQLLNDFKIVLPTLVSFFALLFFLKSQDFGILFWLAALGGGTGVFFL
                     FKKWNLRKHVVLDFYRMVLIIGLVILLTATLFSGATIWPGKSVSGKTVVVTAPTANLR
                     ESPNTSADVVATVQEGERVTVTGKRGSWYRVKAGNRKGWILSDLVST"
     gene            8336..8625
                     /locus_tag="AC16FU_10630"
                     /pseudo
     CDS             8336..8625
                     /locus_tag="AC16FU_10630"
                     /inference="COORDINATES: protein motif:HMM:NF039191.5"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
     gene            9055..9696
                     /gene="tmk"
                     /locus_tag="AC16FU_10635"
     CDS             9055..9696
                     /gene="tmk"
                     /locus_tag="AC16FU_10635"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR2994863.1"
                     /translation="MRKKRGFFITFEGIDGSGKTTQAKKLVRYLKRKRIPVLFLREPG
                     GTNVAEKLRQIVLKATGERIAPAAELFLYLAARAQIVQNRIAEARARGIMVICDRFSD
                     STVAYQAYGRGWNQKLIAELNAMASNSTLPELTLLLDLPVRVGLRRKKTALGGFPYKD
                     RLESEGKSFHRRVRRGYLKTARAEPERIVVIDGTGSPETVWEEIRRVVDKKIA"
     gene            9713..11410
                     /locus_tag="AC16FU_10640"
     CDS             9713..11410
                     /locus_tag="AC16FU_10640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR2994864.1"
                     /translation="MTPIERKQLARGIFFGFLSVVILAAALAYGLGGDLIGRSRLVET
                     IASRIESDYVDYVPADSLIDSGIAGMFNRLDPYSEYLPPANYRSLLEDTQGHFGGLGI
                     EIVVRNNAILVIAPLEGTPAARAGLLPGDRIVKIDGRTAAGMTGEEAVATLRGPQGSA
                     VTIEVKREGLSDPLVLTLKRDEIEVKAVPYAGLTEEGIGYVRLTRFSESAPKELAEAL
                     TFLQTQNPKGIILDLRSNPGGLLGAAVGVAGLFLGGEKLVVETRGRRDEQNKKYTAGA
                     TALCPDLPLVVAVDEYTASAAEIVAGAIQDWDRGLVIGRPTFGKGLVQTPIDLPNGAA
                     LKLTTARYFIPSGRTIQRPDRARALSLVEADSAADSSAHQPFHTKGGRTVYGMGGVVP
                     DLAVERTPRTPVEVALIPYFFDFAVYYLAGHPEVPADFTPDAAVRAQFKKFLAEKKFS
                     YRSQTEDDLALLEGKAAQEGYSLSLRQAIRALRGRINKEKEQALAGESEFISWQIKEQ
                     VLGARYGERAIYPAVWLHRQPELLKAREILTRSGEYRKLLALDSGQSGAIAPPRVAR"
     gene            11413..>11631
                     /locus_tag="AC16FU_10645"
     CDS             11413..>11631
                     /locus_tag="AC16FU_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR2994865.1"
                     /translation="MSGVEPLALVLGGAAAGLFGSLLGLGGGLLIIPYLTLVFNVPMH
                     TAIATSLICVIATSSAAAAVYVQRHLCDI"
BASE COUNT         2369 a         3185 c         3544 g         2533 t
ORIGIN      
        1 ggttgccgat cagaatgacc ttattgacgc tcgccatagg gactccttta cgtcaggtcg
       61 tttgtagcca aataaaatag ggggctgacc cgctggcgtc aatcctgccg gaagggatcg
      121 gcgactcgat ctcttaacct tgcgaaaacc tttggggggt catatctttt ttcgttcaat
      181 gccgaaacga gttatccgag aatcaggtac gccaaaaaac aggctgcggg tggcgctggc
      241 gcaaatcgct ccgaaattgg gggacgtgca ggccaatctc aaaacccacc tcgcgctcat
      301 ccgcaaagcc cgggcgcgga aggcagacct catcattttc cccgaactct ccttaagcgg
      361 ctacctgctg aaggatatgg tgccccgggt ggcgctgaaa ctcgacgcac cggaagtcaa
      421 gcagcttctg acggagagca agtcgattgc catggcggtc ggcctggtag aggaaacgcc
      481 ggagtcgctc tattacaaca gcgccctttt tctggaaaaa ggcaaaatgg tttactgcca
      541 ccggaaagtt tacctgccga cgtacggcat gttcgacgag gggcgcttct tcggcgccgg
      601 tgaagcgctg cgcgccttcg acacccgcgc ggggcggttg ggggtcctta tctgcgaaga
      661 ggcctggcat tcattctgtc cctacctttt gaccttggac ggtgcggtgg ccgtcatcaa
      721 catggccaac agcacggcgc gggggttgga cggaaaaagg cgggtgggca cggctcagac
      781 ctgggagttg atgaaccgct tctacgccgg gatgcaccag gtgtttttca ttttcgtcaa
      841 ccgcgtcggc ttcgaagacg gcataggctt ctggggcggg tcggaagtga tcgatccctt
      901 cggcaaggtg gcggccaaag cgccctactt ccgcgaacag ttagtttttg ccgacatcga
      961 cttggggcag gtggagcagg cgcgcctgcg cacgccgctt ctgcgcgacg agcggctcga
     1021 tttctcttta gccgaattgg cccgggtgcg cgaccagcgg cagaccaccc tcgcttaatc
     1081 ccaagagaaa aatgcgtgtc ggggtgtcga agtatcgagg tgtcgaggtt ggcttcaagc
     1141 gtagtaggaa tttgccttta acttcgacac tccgacactc tccctcgact tcgctcgggg
     1201 cggtgagcct gtcgaaccgc gacacgccga cacttttgaa cattgatccg aaagttacga
     1261 ccgaaatcct caccgctttt ttagtcaacg aagcgggaaa attcggcttc accaaagcgg
     1321 tggtgggcgt ctccgggggg ctcgattcgg ccacggtggc gattttagcg gcccgggcct
     1381 acgggccgaa taacgtgctc ggcgtactct tgccctacaa acactccagt ccggacagtt
     1441 tgaaagatgg cctggctttg gttaggcaga cgaaaatcag gcacgaggtg gtggacatca
     1501 ctcccctggc ggagccgtac atcgaacgat tcaaagcaat cgaccgcgtc cgcattggta
     1561 acgttctggc ccgcttgcgg atgctggtcc tcttcgacct ctcccaccgc ggcgcgtccc
     1621 tggtcatggg cacaagcaac aagaccgaat tgcttttggg ttacagtact tggtacggcg
     1681 acatggcctc ctcgctcaac cccatcggcg acctgtacaa gagccaggtg cgtcagttgg
     1741 cggctttctt gaaagtatcc aagccaatcc agaaaaaaat tcccacggcg gatctctggg
     1801 tggggcagtc ggatgaaggc gaactcggtt tcacctatga cgaagttgac cggctcctgt
     1861 attacctgat tgacgaactg gccacccggg aacaattggt gtccctcggt tttcgagagt
     1921 cactcgtgaa gcgggtggag cggatgatct acaactccca gttcaagcgg tcgctcccca
     1981 taatttgcaa actctcccgc cggacggtgg ggattgattt tcagttgtcg aaggactggg
     2041 ggagataatc ccgaagttgt ggacgtgaat gctttactta gtcgccaccc ccattggcaa
     2101 cctggaagac atttcttacc gtgcgcttga ggtattgaaa agagtcgacc tgattgctgc
     2161 ggaagatacc cgccacaccg gccttttgct caagcacttc ggtatccaag cccggatggt
     2221 gagttaccac gagcacaatg aagcgcggcg ggcggcggag ctgctcgaac ttttaaagtc
     2281 cggcaaaagc attgccatcg tctccgatgc cggcacgccc gggatttcgg acccggccta
     2341 ccggctggtg cagtcagcca ttcaggacca gattcccgtc gttcccattc ccggagccac
     2401 agcgtttgtc cccgcgctgg tggcttccgg cctgcccacc gaccgatttg ttttcgaagg
     2461 ttttctgccc gtcaaaagcg ggcggcgccg gaagcggttg gaagagttgc gcacagaaga
     2521 gcgaaccatc atcttctacg aatcgcccta ccggctggtg cggcttttgg gcgagttgca
     2581 ggaagttttg ggcgaccgga aagcggtggt cgcacgggaa gtgaccaaga agttcgagga
     2641 atttgtgcgc ggaccgctat cagaaattcg cgctcacttt cagctgcacc cgccgaaagg
     2701 ggaaatggtg gtcttagtgg cagggaccaa gaacgctgtt gagccgaggt cggaagcgga
     2761 gtgaatagag tcaaaccaca caacggctgg ttctttctgc cggtggattg ggcgatcctc
     2821 atctacctcg gctcaatttc gctggcgatg ctcttctttc gggagaatgt cccgcactgg
     2881 ccgctctatc tggctttcaa tctcgccgtg agcgggttgg tgttctccct ggcgcggcag
     2941 ttgggaaatc cccagcgggg cttggcccga ttctttcgcc actggtaccc gatatttttg
     3001 ttcacgttct tctatgaaga gaccgggcct ctggtgcaca tcttcttccc caactggttc
     3061 gatatgcacg tggtacatct ggagcgggtg ctcaccggcg gggcgccctc ggtctggctg
     3121 gagcagttcg cctcacggcc actctcggaa tttttgatgt tcggctactt ctcgtactac
     3181 ctgcttttgc cggtcgtcgg cctcatcctc tggctgaaaa ggagagagca ggaattcgac
     3241 cggatgattt tcattgcggc ggtcactttt ttcgtctgct actgggggtt ttacctctac
     3301 ccggtgcagg ggccgcgctg ggcgataggt gattggcatc aggtgccgat ccggggctgg
     3361 atcttcgtgc cggtggtgaa cttcttcatc aacctcgcgg tgcggggcgg ggccatgccc
     3421 tcctcccacg tggcggtggc cttggtggtg ctcctttttg cctggcgcaa cgtccgctgg
     3481 ctgggaataa ttcttacgcc cattgtcgcg ggattggcgg tcgggacagt ttatggacgt
     3541 tttcactacg ctgccgacgt ggtcgtgggc atcgccatcg gctggggctg tgattttgtc
     3601 cttaatcggt ggctggcccg gcgcgcagtt cgcgaaaaac ttcccgcctt cgtgccgggc
     3661 aggatggctc ccgcacgtcc ggcgttcgcg gaacgccgga gcggggccgc cccggagtaa
     3721 cgtgcacccg gaaatcatca aattagggcc gtttgccctg cgctccttcg ggctctttct
     3781 ggcgctctct tttttcgcgg gtctgggatt aataaaatgg cgggcgaaaa agtacggaac
     3841 cgatttcaac cggttggtca acctggcctt cgtggtcatc atctcaggca tcctgggagc
     3901 gcggctcttc tacgtttttt cgcactggag cgagtttgcg gggcatcccc tggacatcat
     3961 caatcccttt cagagcgggc aggtggtggg gattgcgggg ctcacgctct acggcggggt
     4021 cattctggcc attgtttgcg gatcaatcta tctcaaagca gcgcggctgc ccttctggcc
     4081 cacgtttgac attttcgcgc cggccattgc gttcgggatt ttcctcacgc gcatcggctg
     4141 ctttttgaac ggctgctgtt tcgggatgcc gaccgccctt ccgtggggcg tgcattttcc
     4201 ggcagattcg attccggcct actactttcc gggcgccacg ctgcacccgg cgcagctgta
     4261 cgcctcggcc tacgggcttt tgcttttcct tgttctcgtt ccgttagaca accggaaaaa
     4321 attttacggc agcacctttg cctggtttct catcggcgaa tcaatctttc gcttcctttt
     4381 ggaattcgtc cgctacgccg accccgggac gttttttacc cttgacggtt cccgctggac
     4441 ctacaaccag ctggtggcgg tgggattgtt tgcgggcggt gtggcgatgc gcattttttt
     4501 gagaaagagt tttcccttga cggtgcagaa ggcctgacta tattttgcga ttccgattcg
     4561 cacctgaaaa atctcagaac aggcggatag ggaagagtat cttttcatgt acacagagtg
     4621 ccggtcagac cgtgacctct tggcggtgac ctccagttcg gggagatcaa aggcgttgga
     4681 gaatggataa agtgcagccg acggctgcgg caggggagct ggggtcggac gatcaggtga
     4741 tctcgttcaa ggaaaacctg aaacgcgggg cgcacgcggc tctggatcct ctggttcggg
     4801 cgctggcacg gctggggatg cgggccgatc acctgacgat actgggactg gtattcggtc
     4861 tgggtgcggg agtattgttt tttgctgggc ggtttcggct cggcgcgctt ttggcagctt
     4921 tggccgggtt gtgcgacctg ctcgacggcc aggtggtgcg cctgaccaag tcggtgtccc
     4981 gctttggagc gttcctcgat tccacgctcg accggctggc cgaggcggcc attctggtag
     5041 gcattgccgg gttctacatc gcgcacccgg aggtgctggc gaccggcccg gagcccggaa
     5101 gctgggcggt tgctgtctca ctttttgcca tgctcgcgct ggtggggtca tttttggttt
     5161 cctacacccg ggcgcgggcc gaaggattgg ggattgagtg caaggtcggc tggtttgagc
     5221 ggacggaacg gatggttttg atcatcatcg cgggattgtt cggcgtcggg ccggtgatgc
     5281 cgggcgcgct cgtcgttttg gttttactgt cgttcgccac cgcggcgcaa cgggtggcct
     5341 acgtttggaa gaagacccac ggagcaggtt tggatcgata aggaggttcg ttttcatgag
     5401 taaagtgcgt gtggcaatta ttggtgtggg caattgcgct tcgtcgttcg tccagggagt
     5461 ggagtactat aaaaacgcca aagacggcga ctttgtgccg gggttgatgc acgtcaattt
     5521 gggcggctac cacatccggg acatcgaatt ctccgcggcg attgacattg acaagaataa
     5581 agtcggcaag gatttgagcg aggcgatcta caccaaaccg aacaacacct acaagttttg
     5641 caaagttcca aacctgggcg tcaaactggt gcgggggatg acgcacgacg ggttagggct
     5701 gtacctctcc aaaatcatcg aaaaagcgcc ggggccgacg gccgacatcg tcaagacact
     5761 caaggacacc ggcaccgacg tggtggtgaa ctacctgccg gtgggttccg aagaggcgac
     5821 caagtggtac gtagagcagg ttctggacgc aggctgcggt tttgtgaact gcattccggt
     5881 tttcatcgcg cgggagaagt actggcagaa gcgcttccgc gaccgaggcc tgccggtgat
     5941 cggcgacgac atcaaatcgc aggtcggcgc gaccattcta caccgcgtat tagcgcgtct
     6001 ctttcgcgat cggggcgtca aattggagcg aacgagccag ttgaacgtcg gcggcaacac
     6061 cgactttttg aacatgctcg aacgctcacg gctggaatcc aaaaagatct ccaagaccaa
     6121 tgcggttact tcgcaactcg actacaatct gggtgaggag aacgtgcaca tcgggccgtc
     6181 ggactacgtg gcctggctca ccgaccgcaa gtgggcctac atccggctgg agggccgcac
     6241 cttcggcgac gtaccgctca atatcgaact gaaattagag gtctgggact cgcccaactc
     6301 cgccggcgtg gtgatggatg cggtgcgctg ctgcaagctg gcaatggatc ataagctctc
     6361 cggtgccatt gaagcgcctt cggcctactt caagaaatcg ccgccggtgc agtatacgga
     6421 tgaagaagcg cggaggatga cggaggagtt catccaaaaa tatggcagga aggcaaaacc
     6481 ggccagggtg aagaagtaag ccctattccc gggtttctga cccctccctc actctcccct
     6541 cgaaggggag ggaacatttt gggcgaggag gatacggagg agtttatata gaagtatggg
     6601 aagaaggcga aaccggccaa agccaagaag taatcctcct tttgacccca ccctgccctc
     6661 ccctacaggg gagggaaaaa tttgcaaaag gtttcgtagt cgaggattac atcctcggcg
     6721 caaatcgaca ggttagcgtt gcattaagaa ttacgtagac gacggcttgg taggccgccg
     6781 aggttggtac ggtgcgactc tatcgagaaa cctcggctac gaaatgccct taaagcattc
     6841 ctttttttat cagggcaatt catgaattgc cccgacaacc ccaacgggga caagtcccta
     6901 tgctacagat acaaaaatct cctatcgcgg cgcaggttgg tatcccgaat ggaacctgcg
     6961 ccgttttgat tttgggaaat gtaccgggcg acgagacgtc gcccctacaa aggcctgggc
     7021 agacacggtt cgatcccgcc gttggcggga ctcaccgcag gcgggggtct gcccctacat
     7081 caaatcggcg cgggatacgc aaatcattcc gcgctacgat agttcgttcc gaggctggtc
     7141 tgaaacgact ctacgagaag cctcggctat ggaagtaaag agtatctttg ttccataggt
     7201 caggcttcca gccccgcctc tggcgggact tccagcctga cgggagaagt ccaatgtttt
     7261 gcgcgggtgg acgtagacat ccacccatac gtcgtggtac agaataaatc caggcgtaaa
     7321 ctccccccca tttccgccga taatagggta gacggagggt ataccatgcc gactgcccag
     7381 atattgaccg aacgggaaca gaagaagtac ttggcgctcg accaggaagc caaaaaactc
     7441 ttagagttca aaaaagagaa tatccggaag aaccacctga cgccctgccc acagtgcgcc
     7501 acctggatca acttcaaggc caagaagtgc ccgcagtgca cgtcggacat tgctggtcat
     7561 atgcagaagg tccgggagca gttgaagcgg ctggatgaaa tcagcgcgga actgtacgaa
     7621 ctgcacaaga agaaaatgga gttgtaccac gaagggtcgg cgtacaagcc gatctgggag
     7681 cgggtgcaag cctttttctc cgaccagcag ttgctgaatg atttcaaaat cgtcctccct
     7741 accctcgtca gtttctttgc gctcctgttc ttcctcaaaa gccaggattt cgggatactc
     7801 ttctggctgg cggccttagg cgggggcacg ggcgtctttt tcctcttcaa gaagtggaat
     7861 ctgcggaagc atgtcgtcct cgacttctac cgcatggtgc tgattatcgg gttggtcatc
     7921 ctcctcaccg caacgctctt ctccggagcc acgatctggc cgggaaaatc ggtcagcggg
     7981 aagaccgttg tggtcaccgc acccacggcc aatctgcggg aaagccccaa taccagcgcc
     8041 gacgtggtgg ccaccgtcca ggagggcgag cgggtgaccg tcaccggcaa gcgcggctcc
     8101 tggtacaggg tgaaggccgg caaccgcaag ggctggattc tctctgatct ggtgagcacg
     8161 taagcgacct gagtcctccc gcagactgtt ttctttaggc cctcctttca tccggcataa
     8221 agacttttcg tttttttctt tgcgtcagta tattcccgca acagctctac ccggtgcttg
     8281 agataacgtg attcacctgc tccgagaacg cgccacgcct gagcaactgg aagatatgct
     8341 ggcaacgctg gagacatacg tgaaagtggc ggtagatgtc cggcgggatg ttctggcagg
     8401 cggggggatt ttgcacgccg actgtgggga agtattgttg caggatggaa gcaaagcgga
     8461 gatatttggg gtgcagactg gtaccctgag gaacaggtgg ttcgttttac cgcgctgatc
     8521 aacatcaaac cgcgtctcag taatcgggat atggaaataa aagatcccca aatacgtgcc
     8581 cacgttgaat cagtcgtcaa gaaaggtttt gaaggagttc aatgaggaac ccagaaatct
     8641 tgcgcactcg ctatctcagg gatcccctcc ccatgcgttt ggggaatttg gcgtcgagcc
     8701 tggctcgcct ggcaacatgt gttgaacact cccgcagctg gaactcggtg gcaggtatat
     8761 tggatgaaag cctgcaattc attcaatgga ccatgccggc ggcggcccca gagccacagg
     8821 ttctgctggc ggatgtacaa aaagaattga ttcgctggaa gctggaaagg ggcttggagg
     8881 gagatgcaga agcaacccga gcacttgcag acaatggttg aagaagcgcg ggggtgggcc
     8941 caggagatct tagaactgtc cgggcttttg gacgcgcaaa aaagtcccgc gccctaaact
     9001 tcaaagtctt caggtcaatt ttccatccgg gcgttgcccg catatattgg taaagtgagg
     9061 aagaagaggg gttttttcat taccttcgaa ggtattgacg ggtcggggaa gacgacccag
     9121 gccaagaagc tcgtccgtta cctgaaacgt aaaagaatac cggtgctctt cctccgggaa
     9181 ccgggcggta cgaacgtcgc cgagaagttg cgccagatag tgctcaaagc caccggagag
     9241 agaatcgcac cggcggctga gcttttcctt tacctggccg cgcgcgcgca aatcgtccag
     9301 aaccggattg ccgaggcgcg agcaagaggt atcatggtca tctgcgaccg gttttccgat
     9361 tcaaccgtcg cgtaccaggc ctacgggcgg gggtggaatc aaaagctgat agcagaattg
     9421 aatgcgatgg cttccaattc gacgctgccc gaattgactt tgcttttgga tttacccgta
     9481 cgggtcggtt tgcggcgaaa aaaaaccgcc ttaggcggat ttccctacaa agaccggttg
     9541 gaaagtgaag ggaaaagttt ccaccggcgg gtgcggcggg gatacctcaa aaccgcccgg
     9601 gccgagccgg agcggattgt ggtgatcgac ggaaccggtt ccccggagac cgtgtgggaa
     9661 gagatccgca gggtggtgga caaaaaaatt gcatagagcg gatggagggt ttatgactcc
     9721 catagagaga aaacagctcg cccgggggat cttcttcgga ttcctctccg tcgtcattct
     9781 ggcggcggct ttggcctatg gcttaggcgg cgacctgatc ggaagatccc gcctggtgga
     9841 aacgattgcc tcaagaattg agtccgatta cgtcgattat gtgcccgccg acagtttgat
     9901 cgactccggc attgccggaa tgttcaaccg gctggacccc tactccgaat acctgccgcc
     9961 cgccaattac cgctcgctcc tcgaagacac gcagggacat ttcggcggac tggggattga
    10021 aattgtggtg cggaacaatg cgatcctggt cattgcgcct ctggaaggga cgccggcggc
    10081 ccgggcggga ctgctccccg gcgaccggat tgtgaaaatc gacgggcgaa ccgccgccgg
    10141 aatgaccggc gaggaagcgg tagccaccct gcgcgggccg caggggagcg ctgtgacgat
    10201 cgaagtcaag cgtgaggggc tgtccgatcc attggtgttg actctgaagc gggacgagat
    10261 cgaagtcaag gccgtgccgt acgccggtct cacggaggag gggatcggct acgtgcgttt
    10321 gacccgcttt tccgaatcgg cgcccaaaga gttagccgag gcgctcacct ttctccaaac
    10381 gcaaaatccc aagggtatca tcctcgacct gcggagcaac cccggggggt tactgggcgc
    10441 ggcggtgggg gtggccggct tgtttttggg tggcgaaaaa ctggttgttg aaacccgggg
    10501 gcggcgggat gaacagaaca agaagtacac tgccggggcc accgccctct gtccggacct
    10561 gcctttggtc gtggcggtgg atgagtacac cgcctcggcg gcggaaatcg tggccggcgc
    10621 cattcaggac tgggatcgcg gtctggtgat cggccgcccc acgttcggca aaggactggt
    10681 gcagacgccg attgatctgc cgaacggggc ggccttgaag ctgaccactg cccgctactt
    10741 catcccttcc gggcggacga ttcagcgacc ggaccgggcg cgggcgctct ctcttgtgga
    10801 agccgactcc gctgccgatt cctcggcgca tcaaccattt cacaccaaag ggggacgcac
    10861 ggtctacggc atgggcggcg tggtgcccga tctggcggtc gagcgtacac cccggacgcc
    10921 ggtggaagtg gctctgatac cgtacttctt cgattttgcc gtttactacc tggcgggtca
    10981 ccccgaggtc ccggccgatt ttacccctga tgcggcggtg cgagcgcagt tcaaaaagtt
    11041 cctggctgag aaaaagtttt cctaccggag ccagaccgaa gatgatttag ctctcctgga
    11101 gggaaaagcc gctcaagaag gatattccct ctctctccgg caggccatcc gcgccttacg
    11161 cggcagaatt aacaaggaga aagaacaggc gctggccggt gagtcggaat tcatttcctg
    11221 gcagatcaaa gaacaggtgc tcggcgcgcg ttacggggaa agggcaatct acccggcggt
    11281 ctggctgcac cggcagccgg aacttttgaa agcccgggaa attctcaccc gctccggaga
    11341 ataccggaag cttttggcac tcgattccgg ccaaagtggg gcgatcgccc cgcccagggt
    11401 cgctcgctga acatgtccgg cgtagagcct ctggcactgg tcttgggcgg agcggcggcg
    11461 gggctcttcg gttcgctttt gggtttgggc ggcggccttt tgatcattcc ctacctgaca
    11521 cttgtattca acgtgccgat gcacaccgcc atcgccacca gtttgatctg cgtcatcgcc
    11581 acctcttctg ccgcggcagc agtctacgtc caaaggcacc tgtgcgacat c
//
LOCUS       DBNGZA010000500         4506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_312862, whole genome shotgun sequence.
ACCESSION   DBNGZA010000500 DBNGZA010000000
VERSION     DBNGZA010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_312862"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /gene="hisS"
                     /locus_tag="AC16FU_10650"
     CDS             <1..1279
                     /gene="hisS"
                     /locus_tag="AC16FU_10650"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR2994866.1"
                     /translation="LPEEMLPREKILEVMKNTFRKYGFDPLETPAFEFVEVLSGKYGE
                     EGEKLIYKLAYKGGNELALRYDLTVPLARVVAMYPDLVLPFKRYQIQPVWRADNPQLR
                     QGRFREFYQCDVDTIGSASMLADAEIIAIIAEVLGRLGFQQFKIRINNRKILAGLVLF
                     AGVDIKREAEVCRAIDKLGKIGLEGVKSELKEAGIAEKSIAKILDFCSISGDNATVLQ
                     EAQKRLASIPVAQEGLTELQELFGHLSALAVAESNITLDLSLARGLDYYTGPIFEAVV
                     PDLPHLGSVAGGGRYDNLIGLFTGKDLPATGTTVGLDRIFTALQQLERLDKSKSATQV
                     LVCLFDTETQKRALEITQNLRKAGLAAETYLAPDKLKKQLTYADRKKIPIVVIAGPEE
                     MKNNTVTIRNMRTSEQTNISGTKLVSYLQEHLQ"
     gene            1342..2262
                     /locus_tag="AC16FU_10655"
     CDS             1342..2262
                     /locus_tag="AC16FU_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994867.1"
                     /translation="MVTALFLPQAAAGGVKENPKVREYHFFTARSDSAILRAEKKAET
                     TLQTNPGDPAANLELCKILIATGDYDRADDYGEKAVKLEPKNSTCHLWLARAYGMRAE
                     HSNFLKAFFLVKKAKGQFERAAALDSANLAAQFDLFQYYVFAPGIVGGSTKKALAQRR
                     VIARNPNAGAYRFLTDAILHQDKEELDSAEYYYRRAIAADSTQRTPLLLTIAFMYIAD
                     KQFDRGMAALLEYDTTAHNPAAYTDLLAPGFYRNGFLEKTRKEFEDHRKEWKIPELAF
                     PILARMYEREGNRAKAKEYYETAPLKHGER"
     gene            complement(2265..3785)
                     /locus_tag="AC16FU_10660"
     CDS             complement(2265..3785)
                     /locus_tag="AC16FU_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994868.1"
                     /translation="MSSRWVLSVFLITSLSISASAQPLIRGFTRELAIHNLPTGFALK
                     SFDVTGDSVSELLITSSGRFLIFDGKTFNTLFQDSTTPGVNSLSPADVNRDGVTDLVA
                     VSGDSEIVVWYGPDFLNRRIFPVPRGFSTFAVRNREDGQIEFAFGFPHSDSSCGIINP
                     SDCLIEFWGYVLRYTDTTFTFSDSFNVDISPGPYGEMTYIDVIGSGQRDLILVGYYSS
                     YRSAPPYGTYWDLLVERVHGLQLFSVLSFPIGYSCYLNPCNRCSSFLRPILETYATGN
                     IDVDSAIEFVAFVSPTLPCRPSWSFCAYDFSTATKQWERTDSLDIRALFTVDLNRSAP
                     QEILAYEIQAGKPGLVEYRTSDGATLGYSELPFTPSTLLMGTFGSPPAPKVLLAHGDS
                     VVVYGIDVATAVRELPEETALPRQATLFPPSPNPFNGETAITYSIPATGNVSVRIIDI
                     LGREVRTLVAAAQKAGRYQVTWDATDDQGRSVGSGIYFAVLRGGGSAAAQKIVLLK"
     gene            complement(3846..4493)
                     /gene="lepB"
                     /locus_tag="AC16FU_10665"
     CDS             complement(3846..4493)
                     /gene="lepB"
                     /locus_tag="AC16FU_10665"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR2994869.1"
                     /translation="MERPLWRDYAEASVIALVIVFVLRTFVVQAYRIPSQSMEDTLLA
                     GDFVLVNKVAYHFGEPKPGQVIVFKYPLNPDKSYVKRVVALPGQTVAIRNKVLFIDGK
                     PAPSYPGVKHTDSKILPPDFSSRDNFGPIQVPPDNLFVLGDNRDDSQDSRVWGFVPKA
                     NLIGKAMFVYWSWKPDPKAPKWESPYITPLFAIFFYNLTHFPSRMRWDRLGQVVR"
BASE COUNT         1109 a         1230 c         1244 g          923 t
ORIGIN      
        1 cctgccggaa gagatgctgc cgcgcgagaa gattctggag gtgatgaaaa acactttccg
       61 caagtacggt ttcgatcctt tggaaactcc cgcgtttgag ttcgtcgagg tcctctccgg
      121 caagtacggc gaagagggag agaaactgat ttacaaattg gcctacaagg gcggcaatga
      181 actggccctg cgctacgact tgacagtccc tttagcccgc gtggtggcga tgtaccccga
      241 tctcgtcctg cccttcaagc gctaccagat tcagccggtc tggcgcgccg ataatcccca
      301 actgcgtcag ggccgcttcc gggagttcta ccagtgcgac gtagatacca tcggctccgc
      361 ttcgatgttg gccgacgcgg agatcatcgc catcatcgcc gaggtgctgg gccgactcgg
      421 tttccagcaa ttcaagatca ggatcaataa ccgaaaaatt ctcgcggggc ttgtcctttt
      481 cgcgggagtt gacatcaaac gtgaagcgga agtctgccgg gctatcgata aactgggaaa
      541 aatcggactc gagggcgtta aaagcgaact gaaggaagcg ggcatcgccg aaaaatcaat
      601 cgctaaaatc ctggatttct gctccatatc cggagacaat gcaactgtac tgcaggaagc
      661 gcagaaacgg ctggcctcga ttcccgtcgc gcaggaaggt ctgaccgaac tgcaggaact
      721 tttcggccac ctcagcgccc tcgccgttgc tgagtcaaat atcacccttg acctctcctt
      781 ggcccgcggc ctggactact acaccggccc gatttttgaa gcggtcgttc ccgacctgcc
      841 gcacctgggc agcgtggccg gcggggggcg ctacgacaac ctgatcggcc tcttcaccgg
      901 caaagacctc cccgccaccg gaacgacggt cgggctggac cgcattttca ccgctctgca
      961 gcagttagaa cggctggata agagcaaatc ggcgacgcag gttctggttt gtttgttcga
     1021 taccgaaacc cagaagcgcg cgctggagat aacgcaaaac cttcggaaag ccggtctggc
     1081 cgccgaaacg tatcttgcgc cggataaact aaaaaaacag ttgacctacg ccgaccggaa
     1141 gaaaatcccc attgtggtca tcgccggacc ggaggaaatg aaaaataata cagtgacgat
     1201 ccgcaacatg cgtacctcgg agcagacgaa catttccggg acaaaactgg tttcttactt
     1261 acaggaacat ttgcaatagc agtccctatg cggcagcttt tttctcggaa acaaaagtca
     1321 aaaaatttcc tccggattgc ggtggtaacg gccctttttt tgccgcaagc cgctgccggg
     1381 ggagtcaaag agaatccaaa ggtgcgggag taccacttct tcaccgcccg gagcgattcg
     1441 gctatcctcc gtgctgaaaa aaaagcggag actacactgc aaaccaatcc cggcgatcct
     1501 gccgccaatc tggaactgtg caaaattctc atcgccaccg gtgactacga ccgcgccgat
     1561 gactacggcg agaaagcggt caaacttgag cccaaaaatt ctacctgcca cttgtggctg
     1621 gcgcgcgcgt acggcatgcg cgccgagcac tccaatttcc tcaaggcctt ttttctggtg
     1681 aaaaaagcca aaggccagtt cgagcgggcc gcggcgctcg attcggccaa tctggcggcg
     1741 caattcgatc tcttccagta ctacgtcttt gcgccgggca tcgtcggcgg tagcacaaag
     1801 aaagcgctgg cccagcgcag ggtcatcgcc cgtaatccca acgccggcgc ttaccgtttt
     1861 ttgaccgatg ccatcctgca ccaggataaa gaagagctcg attcggctga gtattactac
     1921 cgccgggcca ttgcggccga ctccacgcag cgcactccgc ttttgctcac catcgccttc
     1981 atgtacattg ccgataagca atttgaccgg gggatggccg cgctcttaga atacgatacc
     2041 accgcccaca acccggccgc gtataccgac cttttggcgc ccggtttcta ccggaacggt
     2101 tttttggaga aaacccgcaa agagttcgaa gaccaccgca aggagtggaa aatccccgag
     2161 ttggcttttc ccatcctcgc ccggatgtac gagcgggaag gcaaccgcgc caaagccaaa
     2221 gagtactacg aaactgcccc cctcaagcac ggggagaggt aaatctattt caagaggaca
     2281 attttttgtg ctgcggcact gcctccccct cgcagcacgg cgaagtaaat cccgctcccg
     2341 acagatcgcc cctggtcgtc ggttgcgtcc caggtgacct gataccgccc ggctttttga
     2401 gcggctgcca ccagcgtccg cacttcccgc ccgaggatgt caatgatgcg gacggaaaca
     2461 ttcccggttg cagggatgct gtaggttatt gctgtttctc cgttgaacgg gttaggcgaa
     2521 ggcgggaaga gagtggcctg gcggggtagc gcagtctctt caggcaattc ccggactgcc
     2581 gtcgccacat caatgccgta gaccacaacg gaatccccgt gcgcaaggag gactttggga
     2641 gccggtggcg aaccgaatgt acccatcagg agagtggaag gtgtaaatgg gagttcggaa
     2701 tatcccagcg tggccccatc tgatgttcgg tactcaacga gtccgggttt gccggcttga
     2761 atttcgtaag ccaaaatttc ttgcggcgca ctgcggttga gatcaaccgt aaaaagggca
     2821 cggatgtcga gcgaatccgt tctttcccat tgcttcgtcg cggtagagaa atcgtaggca
     2881 caaaaggacc aagatggacg acaaggaagg gtgggggaga caaaagccac gaattctatt
     2941 gcagaatcaa cgtctatatt ccccgtggca taagtctcta ggatgggtct taggaacgac
     3001 gagcatcggt tgcacggatt gaggtagcaa gaataaccta tcgggaagct cagaacagag
     3061 aaaagttgta gcccgtggac acgttcaact agtaaatccc aataggtgcc gtaaggcggc
     3121 gccgaacgat aggaggaata ataaccaact aagatgaggt cacgttgtcc tgagccaatt
     3181 acatcaatgt aggtcatctc tccgtaaggg ccgggactta tgtctacgtt aaaggaatcg
     3241 gaaaaggtga aagtcgtatc cgtatacctt aggacataac cccaaaattc tataaggcaa
     3301 tcagaagggt taataatccc acaagaggaa tcactgtgcg gaaacccaaa agcaaattcg
     3361 atttgtccat cttccctgtt tcgcactgca aatgtactga aaccgcgtgg caccggaaag
     3421 atccttcgat tcagaaaatc aggtccgtac caaaccacta tttcactatc accgctgaca
     3481 gcgacaagat cggtgactcc atcccggttc acatccgccg ggctgagact gtttactcct
     3541 ggagtagtgg aatcctgaaa cagcgtatta aaggttttgc catcgaaaat cagaaaacgt
     3601 ccgctacttg tgatgaggag ttccgaaacg ctgtctccag tgacgtcaaa ggatttaagc
     3661 gcgaagccgg tcggcagatt atggatcgcc aattcacggg taaagcctct tatgagaggt
     3721 tgtgcggaag cgcttattga gagcgaagta attagaaaga cacttaaaac ccaccgtgag
     3781 gacatattga ctcctccagt ggaacttaat ataacctaaa gattctattc agacaaggga
     3841 atcttttacc gcaccacctg gcccagccgg tcccagcgca tacgcgacgg gaagtgggtc
     3901 aggttgtaga agaagatggc gaaaagggga gtaatgtagg gggattccca tttgggtgct
     3961 ttggggtcgg gcttccacga ccagtagacg aacatcgctt tgccgatcag gttggccttg
     4021 ggcacaaacc cccagacacg ggagtcctgg ctgtcgtcgc ggttgtcgcc cagaacgaag
     4081 aggttatccg gcggcacctg tatcgggccg aagttgtccc ggctggaaaa gtctggcgga
     4141 aggatcttgc tgtcggtatg tttgacgcca ggataggagg gggcgggctt gccgtcgatg
     4201 aagagcacct tgtttcggat tgcgaccgtc tgccccggca gggccaccac ccgcttgacg
     4261 taacttttgt cgggattgag cgggtacttg aagacgatca cctgacccgg cttgggttcg
     4321 ccgaagtggt aggccacttt gttgaccaag acgaaatcac cggccaaaag ggtgtcctcc
     4381 atcgattgcg aagggatgcg ataggcctga acgacgaaag tgcgcaggac aaagacgata
     4441 accagcgcaa tcaccgacgc ttcggcgtag tcccgccaga gcggccgctc catccggcgg
     4501 tagcgc
//
LOCUS       DBNGZA010000501         7127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2858195, whole genome shotgun sequence.
ACCESSION   DBNGZA010000501 DBNGZA010000000
VERSION     DBNGZA010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2858195"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="AC16FU_10670"
     CDS             complement(<1..703)
                     /locus_tag="AC16FU_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR2994870.1"
                     /translation="MRAFRLVVVTFFWFTGAAVAQVPPRDWQEDFSWRGAERPKIGKI
                     YLTGNQKFPTGLLREKLFSREASWLVSFGLARATRLQKDSRERDLVNLLYSYQSNGFL
                     EARITERFGWDSTARRVDIWVEIGEGVQTKIGPVTVAGDTAGFSEKIFKLTTAMKSGE
                     PFNPFVLEQTRSGVKTLLADNGYPYARVSVDTLRDSTLPNLGIQFRIARGQEVHFGRV
                     TVEGLQYTRPHVATRE"
     gene            complement(751..4293)
                     /locus_tag="AC16FU_10675"
     CDS             complement(751..4293)
                     /locus_tag="AC16FU_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016260.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HIR2994871.1"
                     /translation="MRRRYKIPLFLLAALGLFILGAYLVLTRTSLLKSQINRYLAGYL
                     SSQFPLEVKVGKVGGVPWKSIVLEDVTVDYTVKPHAYRMLTIDRIEAHYRLSNVWNQE
                     WIASRLDLVGVKAVLKQDENGDILFPQYLGNQKKGKAELNFQVDTLRVADGSFEFFTP
                     QRSYKADSLTLLGRCSGSGTTIDFAVSDLRFALPQERIRTQKALFAGQYREGRLWLDS
                     ALIQTDSSLLAFGGTLVLAERPQLSAKVERGRIDFGEFKRLTGFDLQGDVAFSGRVKS
                     DFHETRGQTKLSGIFFGHTLNNLAVDYLYQQKTLFCSALSGEAAGTQLSGRGKFDFTG
                     KPERYEFSGRVNHLDLRRWVSTDLASDFNGEVQLVGAGLSDKTLRLNLTVALDSGRLD
                     RYRFHQVRGALTLDAHRATFADDFTVRYKNAAFTFAGSVGFTDSLNLHGTIDLPDLSD
                     FRGQTFIKEPAGRGEATFLATGRTLDPDIQGTFWSDSCWLYQLFSSRLEGVYAVRRFE
                     TARQGRIRLSLANGTAWDVPYDSLSAVFTLDSTRAYFDSARFWRADLYVQGDGVIDYA
                     DSLVTKLTVQGLSGDYRGNAFGLGRPARISVDTAGYDLNDVLLENGSGTVAVKGRIDY
                     SEKMDLDLTGQNAALGPWTRFFFRKKEEVGGLLAFSGHLAGDFTHPKFNLEFRADSLT
                     YGKARLGNLAGKVLYQNQKLTLEDLRLTSQAAHYTWSGFIPLDLAFTAVPHRVLEAPQ
                     EIRFTALGQRFDLVHFFVENIEWLEGHFSADILFTGTPDAPQAHGKVELQNGTCKTLP
                     LVNPVKDVNLVLRLENETVTLEKATGTLEWKRQKGELEAAGTVIILSPQEFDYHLAVT
                     GQGVPIRYEFDEFEGTGNFRLAVTGSWPPLVSGDISVTSAVYSGAFAEPAPAGVVVAN
                     GTAQAASPPWDLQLTVDLPGSFRVSNEETNTEFKGKLVVLRENGIYNYLGELETVRGK
                     FYTIGRTFTITQGKITYDNISVPDPKLDITAVTRIRRFERDTAFTSSRPADIDLVIQI
                     TNTLSHPVIKPASGSPFSEEDLVLLLGTGGVFNPAATSSGDRKTGISNRLTIGGVNFA
                     SAVLSQYAARQFGIETLELSPVSAGQFDLRQTELLVGKYTFPNFYLYGRSRFASLKEQ
                     EVGFEYRINRWLSLEGVRDRANLYRFNLNANWEY"
     gene            complement(4431..5846)
                     /locus_tag="AC16FU_10680"
     CDS             complement(4431..5846)
                     /locus_tag="AC16FU_10680"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR2994872.1"
                     /translation="MSKLFWIAAGLFFGFIWTNIVTLPEAEAQRTLSNLEEDINALLE
                     GVKPAIVTVEAKHTWEGEPGAKKKKIESEASHRFIGSGIIYNDKGYILTTPNVLQSGP
                     NVLQSGSFQLLFGDQPARSQEKYQVTLGDGRSFSAELIGQDEETNLAVLKVEAANLHP
                     ARLGNSGLLKEGSWITVVGNAYGVPAGVSFGLVNGFRDDGTIQMSVNVSPGSSGGPVL
                     NTKGEVVGLVLAKVSEPFFISLSGDDNKKSTTVKRYSEQLDLPTSGVSLAVPIDRVRE
                     VADQIIAKGKVERAYAGVYPLDLDDDTKENFDLKAGALISEVVAGGPADKAGIKEGDV
                     VTALDGEAVKNEADFRQKMSRLKPDTKATFTVRRKVSGREDQTKEIAVTLGSRSAAAA
                     VPARGDARGWEEFGRALGNLRNYSFNREEETLSKAEKESLQKQLEKLQEQMEKQQELF
                     QSQMEKLQEELSRLQEQKGKE"
     gene            complement(5889..6596)
                     /locus_tag="AC16FU_10685"
     CDS             complement(5889..6596)
                     /locus_tag="AC16FU_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994873.1"
                     /translation="MRCRTARRYLLISAEGGTGSAQVTAHAAVCPACRAYAASLAHLR
                     HSAAGIDPQTVPDGFTDRLLVRLRETPKREVQLVPERSRAIAWRWLPVPIALALVAAG
                     GIYLGSGHRTKMRSGPTIPPVAIAPQAAQLPLPPRAVAPDKPSAAENKAELPAPPQRQ
                     LASSRLEESPPTQISERSAPDLSGMIPDGFWFISARDQKNYLMPGVDLNARRLREAQP
                     VYVLPVVATERYVYRDY"
     gene            complement(6553..>7127)
                     /locus_tag="AC16FU_10690"
     CDS             complement(6553..>7127)
                     /locus_tag="AC16FU_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR2994874.1"
                     /translation="ELFRAVQKGDQTAFRNLVERYKDRLFNVVVRLLADREEASDVVQ
                     ETFLRVFQHRERYNPAAAAVSTWIYTIALNLARNELRRRKKMKFLDIFELAQSDGDLR
                     WLSPAPGGWKQALEKAIAHLPVKYRTCFLLRDVDGWSHEEIAQMLAVPVGTVKSRVAR
                     AHHLLQKELKPRLEEINALSHGTPLPVDLR"
BASE COUNT         1437 a         2143 c         2053 g         1494 t
ORIGIN      
        1 gttcccgcgt ggccacgtgc ggccgggtgt actggagccc ttccaccgtc acccttccga
       61 agtggacttc ctgcccccgg gcaatccgga attgtatccc cagattcggc aaggtgctgt
      121 cgcgcagagt gtcgaccgaa acgcgggcgt acgggtagcc gttatcggcc aacaaggttt
      181 tgacgccgct gcgggtttgt tccagcacaa aagggttgaa gggctctccc gatttcatgg
      241 cagtagtgag tttgaaaatt ttctccgaga aacccgccgt atcgccggcg acagtgaccg
      301 ggccgatttt cgtctgcaca ccttcaccaa tctccaccca gatatcgacc cgccgggccg
      361 tcgagtccca gccgaatcgt tcggtgatgc gcgcctccag aaagccgttg ctctggtagg
      421 agtacagaag gttgaccaga tcccgctccc gggaatcttt ctggagtctc gttgcccggg
      481 ccagaccaaa ggagacgagc cagctcgctt cccgggaaaa gagtttttca cgcaggagac
      541 cggtcgggaa cttttgattg ccggtgaggt agatctttcc gattttcggg cgctcggccc
      601 cgcgccaaga aaaatcttcc tgccagtcac gcggcggcac ttgcgctact gccgctccgg
      661 tgaaccagaa gaacgtaact acgaccagcc gaaaggcgcg cacccgtgtc agaatagaat
      721 aaaggcggaa aactggcaac ggccggggct ttagtactcc cagttggcgt tcaggttgaa
      781 gcggtacaaa ttggcgcggt cgcgcacccc ttccaaggag agccagcgat tgatccggta
      841 ctcaaaaccc acttcctgtt ctttgagcga ggcaaaacgc gagcggccgt acaggtagaa
      901 gttcggaaag gtgtacttgc cgaccaaaag ttcggtctgc cgcaggtcga actgtccggc
      961 ggataccggc gaaagttcca gcgtctcgat cccgaattgc cgcgccgcat actggctcaa
     1021 aactgccgat gcgaaattga cgccgccgat ggtcagccgg tttgaaatcc cggttttccg
     1081 gtcgccggag gaggtcgccg ccggattgaa cactcccccg gtgcccagga ggagcaccag
     1141 atcttcttcg gagaaaggcg acccggatgc cggtttaatg accgggtggc tcaaggtatt
     1201 agtgatttga atcacgagat caatgtctgc cggccgggag gaggtaaagg ccgtgtcgcg
     1261 ttcgaaccgg cggatgcggg tcacggccgt aatgtccaat ttcgggtcgg gcacgctgat
     1321 gttatcgtag gtgatcttcc cctgggtgat ggtgaaggtg cggcctatcg tgtagaattt
     1381 cccgcggacg gtttccagtt cacctaagta attatagatg ccgttctcac gcaagacgac
     1441 cagtttccct ttgaattccg tgttggtttc ctcgttggac acccgaaagc taccgggtaa
     1501 atccaccgtc aactgcaaat cccagggagg actggcggcc tgggcggtgc cgttggcaac
     1561 cacgacgcct gcgggcgccg gttcggcaaa cgcgccggaa taaaccgccg aggtgacgct
     1621 gatgtcgccg gaaaccagag gcggccagga gccagtgacc gccagccgga agttgcccgt
     1681 gccttcgaat tcgtcaaact cgtagcggat cggcaccccc tggccggtta ccgcaaggtg
     1741 gtagtcgaac tcctggggtg agagaatgat gaccgtgccg gcggcctcga gttccccctt
     1801 ctgccgcttc cactccaacg ttccggtcgc tttttcgagg gtgaccgtct cattttccag
     1861 ccgcaagacg aggtttacgt ctttcacggg attaaccaga ggcagagttt tgcaggtgcc
     1921 gttctggagt tcgactttgc cgtgtgcctg cggagcatcg ggcgtaccgg tgaaaagaat
     1981 atcggctgag aaatgccctt ccagccattc aatgttttcc acaaaaaaat gaaccagatc
     2041 gaacctttgg cccagggcgg tgaaacggat ttcctggggt gcctccagaa cccggtgggg
     2101 cacggcggta aaggccagat cgagcgggat gaaacccgac caggtgtaat gggccgcctg
     2161 gctcgtcaag cgcagatctt cgagggtcag tttttgattt tggtataaaa ctttcccggc
     2221 aagatttccc agccgggcct tgccgtaggt caaactatcc gcccggaatt cgagattaaa
     2281 ttttggatgg gtgaagtccc ccgccagatg gccggagaaa gccaaaagtc ctcctacttc
     2341 ttctttcttc cgaaagaaga atcttgtcca gggaccgaga gcagcgttct ggccggtgag
     2401 atctaaatcc attttctcgc tgtaatcaat gcgccctttt accgccaccg ttccggaacc
     2461 gttttcaagc agcacgtcat tcaggtcata acccgccgta tcaacagaga tgcgggcggg
     2521 tctgccgagg ccaaaggcat tcccgcggta atcgccggag agaccttgaa ccgtcagttt
     2581 ggtgaccagt gaatcggcgt agtcgatgac cccgtcacct tggacgtaga ggtcggcgcg
     2641 ccagaaccgc gccgaatcaa agtacgctcg ggtactatcc agcgtgaaaa cagccgacag
     2701 cgaatcgtac ggcacgtccc aggcggttcc gttcgccaga gaaaggcgga ttctcccctg
     2761 ccgcgccgtc tcaaaccgcc gaacggcata cacgccttcc aggcgggaac taaagagctg
     2821 atacagccag caggagtcgg accagaaagt gccctgaata tcaggatcaa gcgtcctgcc
     2881 ggtggcaaga aaagtcgcct ctccccggcc ggccggttct ttaatgaagg tctgaccccg
     2941 gaaatcggaa agatcgggta ggtcgatagt gccgtgtaga ttgagcgaat cggtgaaccc
     3001 caccgatccg gcaaaggtaa atgccgcatt tttgtagcgg acggtgaaat catcggcgaa
     3061 agtcgcccgg tgggcatcca gggtaagtgc tccgcggact tggtgaaacc ggtacctatc
     3121 caggcgtccg gagtcgagcg ccaccgtcag gttgagccgg agcgtcttat ccgacagccc
     3181 cgcaccgacg agctgtactt ccccgttgaa atcgctggcg aggtcggttg agacccagcg
     3241 gcggagatcg aggtggttca ccctcccgct aaactcgtac cgttccggtt tgccggtgaa
     3301 atcgaatttc ccccggcccg acagctgcgt cccggccgcc tcgccggaaa gtgctgaaca
     3361 gaaaagcgtt ttttgctgat agagatagtc gacggccaaa ttattcaacg tatgcccgaa
     3421 gaaaatgccc gagagtttgg tctggccacg agtttcgtgg aaatccgatt ttacccggcc
     3481 gctgaaggcc acgtcccctt gcagatcaaa accggtcaga cgcttgaatt ctccgaagtc
     3541 aatgcggccc cgctcgacct tggcggagag ctggggccgt tcggccaaaa ccagtgtccc
     3601 gccgaaggcc agcagactgg aatcggtctg gattaaagct gaatccaacc agaggcgacc
     3661 ttcccggtac tgaccggcaa agagcgcctt ttgggtgcgg attctttcct gcggcagcgc
     3721 aaagcgcaag tcggaaacgg cgaaatcaat ggtcgtgccg ctgccgctgc agcgccccaa
     3781 aagggtcagg ctgtccgcct tgtaggaacg ctgaggggtg aaaaactcaa acgaaccgtc
     3841 cgcaaccctt agcgtatcga cttgaaagtt gagctcggcc ttcccctttt tttgattccc
     3901 gagatactgg ggaaacagga tgtctccatt ttcgtcctgc ttgagcaccg ctttgacgcc
     3961 gactaaatcg aggcggctgg caatccactc ctggttccag acgttggaga gccggtagtg
     4021 cgcttcaatc cggtctatgg tgagcatccg gtaggcgtgg ggcttgactg tgtaatcgac
     4081 cgtcacgtct tccaacacga tcgatttcca cggcactccc cccaccttcc ccactttcac
     4141 ctccagcggg aactggctgg agaggtagcc ggccaggtag cggttgatct ggcttttgag
     4201 cagggaggtc cgcgtcagca ccaggtaagc tcccagaatg aagagcccca gtgccgccag
     4261 gaggaacaga ggtatcttgt agcgccggcg catccctagt attccctacg gataaaaatc
     4321 ggaaactggc gaggtaaaat caacgtcaat ctttccccgc gacgcggaaa gcaaagcgcc
     4381 ccgcggggac ggggcgctgc ggttattctt tttcagccgg cgagaaacgg ctactccttg
     4441 cctttctgtt cctgcaggcg ggagagttcc tcctggagtt tttccatctg tgattgaaaa
     4501 agctcctgct gtttttccat ctgctcctgg agtttttcca gttgcttctg gaggctttcc
     4561 ttttctgctt tggataacgt ctcttcttcc cggttgaaac tgtaattccg taaatttccc
     4621 agggcgcggc cgaactcctc ccacccccgg gcatctcccc gggccggcac cgccgcggcc
     4681 gcggagcggc tgcccagggt cacggcaatc tctttcgtct ggtcttcccg tccggagact
     4741 tttcgtctga ccgtgaaggt cgccttcgta tccggtttca ggcggctcat tttctgcctg
     4801 aagtcggctt cgtttttcac cgcctcgcca tcgagggccg tgaccacatc accctctttg
     4861 atgcccgcct tgtctgccgg ccctcccgcc accacctccg agatcagcgc cccggctttg
     4921 agatcgaagt tctcttttgt gtcatcgtcc aagtccaggg gataaacgcc ggcgtaggcg
     4981 cgctccactt ttcccttggc gatgatctgg tcggccactt cccggacgcg gtcaatcggt
     5041 accgccaggc tgacccccga agtcggcaga tccagctgct cgctatagcg tttgaccgtg
     5101 gtgctcttct tgttatcgtc gccggaaaga gagatgaaaa acggctcgct gaccttcgcc
     5161 agcaccaggc cgaccacttc ccccttcgtg ttcaaaaccg gcccgccgga agagccgggg
     5221 gacacgttta ccgacatctg aatcgtgccg tcatcccgaa aaccgttcac gagcccgaaa
     5281 ctcacccccg ccggaacgcc atacgcattt cccacaacag tgatccagga cccttccttc
     5341 aagaggccag aattccccag ccgcgcgggg tgcaggttgg cggcctcgac tttcaagacc
     5401 gccagattag tttcctcgtc ctggccgatc agttccgccg aaaacgaccg gccgtccccc
     5461 agggtcacct ggtacttttc ctgcgagcgt gccggctgat cgccgaaaag aagttggaag
     5521 gaaccggact gcagcacgtt ggggccggac tgaagcacgt taggggtggt caggatgtag
     5581 cccttgtcgt tgtagatgat cccggagccg atgaagcggt ggctggcctc ggattcgatt
     5641 ttcttcttct ttgctcccgg ttcgccctcc caggtgtgtt tggcttccac cgtgacgatg
     5701 gccggcttga ccccctctaa gagggcgttg atgtcctctt cgagattgga aagagtccgt
     5761 tgggcctccg cctcaggcag cgtgacgatg ttcgtccaaa tgaacccgaa gaacagacct
     5821 gcagcgatcc agaatagttt gctcattgac attcctcctc ctttcgcact ggtaccttca
     5881 ttcccttctc agtaatcacg gtaaacgtat ctttcagtgg ccacgaccgg caatacgtac
     5941 accggctggg cttcgcgtag ccttcgggcg ttcaaatcga ctcccggcat caggtagttc
     6001 ttttgatccc gagcagagat gaaccagaag ccgtccggaa tcatcccgga cagatcaggc
     6061 gccgaccgct ctgagatctg ggttggcgga ctttcttcca agcgggacga agccagctga
     6121 cgctgagggg gggccggaag ctcagcttta ttctctgcag cggaaggttt atccggcgca
     6181 actgcccggg gcggcagtgg cagctgtgcc gcctgggggg cgattgcgac cggcgggatg
     6241 gtcgggccgc ttctcatttt tgtccggtgg ccgctcccca gatagatgcc acccgcagcc
     6301 accagagcca gggcaatcgg aaccggcagc caacgccagg caattgctct ggatcgctcc
     6361 ggcaccagtt gcacttcccg cttcggcgtc tctcgcaacc ggaccagcaa gcggtcggtg
     6421 aagccatccg ggacggtctg cgggtcgatc ccggctgccg agtggcggag gtgagctaaa
     6481 gaggccgcgt aggcgcggca ggccggacag acggctgcgt gcgccgtaac ctgagccgaa
     6541 ccagttcctc cctcagcgga gatcaacagg taacggcgtg ccgtgcgaca gcgcattgat
     6601 ttcctccaac cttggtttca actctttttg cagcaaatgg tgcgcgcggg cgacccgcga
     6661 tttcaccgtt cccacgggga ctgcaagcat ctgcgcgatt tcctcgtggg accagccatc
     6721 cacgtctcgc aggagaaaac aggtgcggta cttcaccggc aggtgggcta tcgctttctc
     6781 cagggcctgc ttccagcccc cgggcgcagg cgaaagccag cgcaggtcac catcggattg
     6841 ggccagttcg aagatgtcca aaaacttcat tttcttccgc cgcctcaatt cattgcgggc
     6901 cagattcagg gcaatcgtgt agatccaggt cgaaaccgcc gccgccgcgg ggttgtaccg
     6961 ttcccggtgc tgaaaaaccc gcaggaaggt ctcctgcacc acgtctgagg cttcttcccg
     7021 gtcggccaga agccgcacca ccacgttgaa caagcggtct ttgtagcgct ccaccagatt
     7081 ccggaacgcc gtctggtcac ctttttggac cgcccggaac aactccc
//
LOCUS       DBNGZA010000502         4878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1266098, whole genome shotgun sequence.
ACCESSION   DBNGZA010000502 DBNGZA010000000
VERSION     DBNGZA010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1266098"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16FU_10695"
     CDS             <1..539
                     /locus_tag="AC16FU_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HIR2994875.1"
                     /translation="KKTQSDQEPNEIGYQTGFFLADPFSLTGDFNLEYTRVANRTYNQ
                     ALPYNRYLNRNQSLGYPSGPDGEHFFVSFRKWLPGAQALTGTYQYHRQGEGKIAAPWT
                     TPWLSVSGAYHEPFPSGVVERTDEVSLLWQYAYRRNVRLEIETGFRQVKNLAHQAGQT
                     ERGWLVGLGLQIDGVWTK"
     gene            654..1442
                     /locus_tag="AC16FU_10700"
     CDS             654..1442
                     /locus_tag="AC16FU_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994876.1"
                     /translation="MKTLKQILAALTLGLALVVILGLRCHRIAGPGGTPQPTQVYRID
                     GVAVRDMNSTDQVWLRVRRSGALFDSATVLFDTVAVPRIASGEYQRLSPGVNLTPGNH
                     TLAVRYAPDNLNLSLALVMPDTFVIDSSSLDATERQYRGIPATVTLDWSPSVNAQGYL
                     VAANRETPVRDNFQSFLQLSVGANSFGFPRNTWRDTLADTVLTGLYRIHVASYRPTLV
                     SYPGMPFVLPALGLSDNINSPNLTGRFGASVIAPFDTIRVITQF"
     gene            1571..2479
                     /gene="murQ"
                     /locus_tag="AC16FU_10705"
     CDS             1571..2479
                     /gene="murQ"
                     /locus_tag="AC16FU_10705"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR2994877.1"
                     /translation="MKDLVTEAFNPRTKKLDRLTTVAILQRINREDRTVPSAVAKEIP
                     SIARAVELYVAALGSGGRVFYVGAGTSGRLGVLDAAECPPTFGTDPKKIQGVIAGGFR
                     TLIRSREGVEDRRQAGAKDLRRRKLTKRDIVVGITASRRTPYVLGALAYARRVGARTV
                     FICANPRRMVKVKANIVICLVVGPEVIAGSTRMKAGTAQKLVLNMLSTAAMVRLGKVY
                     GNVMVDLRATSEKLVERSKRILMLLGKISYPQAEKLLRAAGGSVKVALVMRLTGLSRR
                     EAEKRLAKAGGFVYKVVAEQKKKSAR"
     gene            2532..2870
                     /locus_tag="AC16FU_10710"
     CDS             2532..2870
                     /locus_tag="AC16FU_10710"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR2994878.1"
                     /translation="MAYCPKCQAEYKDGRDECIDCGVPLVEAMDELEETEDSGGFVPL
                     RTLPSRMYAQMLKESLEKAGIPALIKGEDIGIMLGNFGTTSTVPVELWVPADEVDKAE
                     AIAAGMMGEK"
     gene            2978..3232
                     /locus_tag="AC16FU_10715"
     CDS             2978..3232
                     /locus_tag="AC16FU_10715"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR2994879.1"
                     /translation="MSAAFVLLRRVESRPEALVIQGVLETAGIPVVVRTPDKYLLLQG
                     DWSGSPKPPPVEIWVAGERKAEAEELLKSPSGLTDDTSSI"
     gene            3584..>4878
                     /locus_tag="AC16FU_10720"
     CDS             3584..>4878
                     /locus_tag="AC16FU_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HIR2994880.1"
                     /translation="MLKNYVSRVFKVSQRGDAREESYYSALESLLRDYAESVGKRGTD
                     VTTLPKKTEGGNPDFRVWDGKEKIVGYIEAKDPSVAHLDQIETSEQLKRYLGIFPNLI
                     LTNFHEFRLYRNGDLIDQVLIARPVIGRKLKVVPPVEREEKFLQLLEKFFSFSLPAIT
                     DAKTLAEELAKRTRFLRDEIISEELKAGKGRVAGFYEAFKKYLIGGLTKEDFADLYSQ
                     TITYGLFASRTRSENGFNRRLAYYNIPATIGILRDVFKYISLEDPPQQMEWIIDDISQ
                     ILANTKVNDILHRYFHEGKGKDPIIHFYETFLTEYDPDTRERRGVYYTPEPVVSYIVR
                     SLHKILKEEFNRPDGFASTSVTVLDPAAGTLTFLAEAAKVAVDEFVEKYGEGGKQNLI
                     KEHILKNFYAFELMMAPYAIGHLKMSFLLEELGYRLQKD"
BASE COUNT         1153 a         1282 c         1415 g         1028 t
ORIGIN      
        1 aaaagaagac gcaaagcgat caagaaccca atgaaatcgg ctaccaaacc gggttcttcc
       61 tggccgatcc tttttccctg acaggtgatt tcaacctgga gtacacccgc gttgccaacc
      121 ggacgtacaa tcaggcattg ccctacaatc gctacctcaa ccggaatcaa agtctggggt
      181 atccctccgg gccggatggc gagcattttt ttgtttcttt ccgcaagtgg ctgcccggag
      241 cgcaggcgct caccggaacc taccagtacc accggcaggg ggaagggaaa attgccgccc
      301 cctggacgac gccgtggctt tcggtcagcg gcgcgtacca cgaaccgttc ccgtcgggag
      361 tggtggagcg caccgacgaa gtatcgcttt tatggcagta tgcctaccgg cggaacgtcc
      421 ggctggaaat cgagaccgga ttccggcagg tcaaaaatct ggcacaccag gcgggccaga
      481 ccgaacgcgg atggctggtg gggctggggc tgcaaattga cggcgtttgg acgaagtagc
      541 cggcttgtgc ggactccagc accgcaagca gatcatatca ataaagtgtt ccagaaagcc
      601 gatatagaga taaaacagta cggagacaac cgcactcaag gaaagaagaa aagatgaaaa
      661 ctctaaagca gattctggcc gctcttaccc tcggccttgc gcttgtggtc atcctcggcc
      721 tgcggtgtca ccggattgcc gggccgggtg gcacgcccca gccgacgcag gtttaccgga
      781 tagacggcgt ggccgtacga gatatgaaca gcaccgatca agtgtggctg cgggtgcggc
      841 gcagcggcgc cctttttgac agcgcgacgg ttctcttcga cacggtcgcc gtcccgcgaa
      901 tcgcctccgg cgaataccag cgcctttccc cgggagtaaa cctgactccg ggtaaccaca
      961 cccttgcggt gcgctatgct ccggataatc tcaatctctc actggcactg gtgatgcccg
     1021 atacgttcgt gatcgacagt tcatccctgg acgctacgga gcggcaatac cggggcatcc
     1081 cggcgaccgt gaccctggac tggtcgccct cggtgaacgc tcaaggatac ctggttgccg
     1141 ccaaccggga gacgccggtg cgggataact ttcagagttt cctccagctc tcggtaggcg
     1201 ccaacagttt cggctttccc cgcaatacgt ggcgggatac gctggccgat acggttctca
     1261 ccggcctcta ccgcatccac gtggcctcct atcgcccgac tttagtgtcg taccccggga
     1321 tgccgttcgt cctgccggca ctgggtttga gcgacaacat caacagcccc aacctgacgg
     1381 gccgcttcgg ggcgtcggta attgcgccgt tcgataccat ccgggtgatc acccagtttt
     1441 gagcttgcgc acgcaaagcg gattctgtaa catcacccga gacttgtggt ggtccggtaa
     1501 atcgagtgag tgacctcgcc tcaggcggtg cgaagaggag tcgcccctgt cccgaaaagt
     1561 tttcaacgaa atcaaagacc tcgtcaccga ggcattcaat ccccgaacca aaaaattaga
     1621 ccgtctcaca accgttgcca tcctccagcg gattaaccgt gaagatcgca ccgttccctc
     1681 cgccgtcgcg aaagaaattc cttccatagc cagagcggtt gaactctacg tagcggcgct
     1741 gggctccggc gggcgggtgt tttacgtcgg cgcaggcacc tccggccgtc tcggcgtgtt
     1801 agatgccgcc gagtgcccgc cgacttttgg caccgatccg aaaaaaattc agggagtcat
     1861 tgccggaggt ttccgcaccc tcattcgctc gcgcgaggga gtggaagaca ggcggcaagc
     1921 gggagcgaag gatctccgca ggagaaaact gaccaaacgt gatatagtcg tgggcatcac
     1981 cgcctcgcgg cggacgccgt acgttttagg tgcgctcgcc tacgcccgcc gcgtcggtgc
     2041 tcgaaccgtt ttcatctgcg ccaacccccg gagaatggtc aaggtgaaag ccaacattgt
     2101 catctgcttg gtggtagggc cggaggtgat tgccggctcc acgcgaatga aagccggcac
     2161 cgcgcagaag ttggtgctca atatgctctc gaccgcagcg atggtccgct tgggcaaagt
     2221 ctacgggaat gtgatggtcg atctgcgggc gacttccgag aaattagtcg agcggtcgaa
     2281 gagaatctta atgcttctgg gaaaaatttc gtacccccag gcggaaaaac ttttgcgcgc
     2341 ggcgggcgga agcgtgaaag ttgccctcgt tatgcgcttg accggtcttt cgcgccggga
     2401 agcggaaaag cggcttgcca aagccggggg ctttgtgtat aaagtagttg cggagcagaa
     2461 aaagaagtcg gcacgttagg gtgagttcag taaaggtatt tgcagcggga tgagcaggtg
     2521 gaggattggc aatggcgtat tgtcccaagt gccaggcaga gtacaaagac ggccgcgacg
     2581 agtgcatcga ctgcggcgtg ccgttagtcg aggcaatgga cgaattggaa gaaaccgagg
     2641 acagcggcgg gtttgtgccg ctgcggaccc tgccctcccg catgtacgcg cagatgctga
     2701 aagagtcgct ggagaaagcg ggcattccgg ctctgatcaa aggcgaggac atcggcatta
     2761 tgttagggaa tttcggcacc acttccaccg tgccggtgga actctgggtg ccggcggatg
     2821 aagtggacaa agccgaagcg attgccgccg gaatgatggg ggagaagtaa gccgggttta
     2881 tccgatcgga atattgcccc gcatttcgta ttcaggaagc tcttgcagtt cgccactcaa
     2941 ctccatttcc atttgacaat tttttgagac gaggaaaatg tcagcggcat tcgttttgtt
     3001 gcgccgggtg gaaagccgtc ccgaagcgtt agtcattcag ggcgtcttag agaccgccgg
     3061 gattccggta gtcgtgcgga cacccgataa ataccttctc ctgcagggcg attggagcgg
     3121 ttcgcccaaa ccgccgccag tggaaatctg ggtggcgggg gagcggaaag ccgaagcgga
     3181 agagctcctc aaatccccaa gcggattgac cgacgacacc agttctattt agtgccttct
     3241 tgcggggtgc ggtagacaac gagccgagcg gctggaagtg gaaagtggag tagggaaagg
     3301 gtcgttgaaa accctgccat cggctctgaa cgagagcaaa accgaaggga ggtttacgct
     3361 ggtgctgccg gtgtaaaagc aaaacacccc ctgggcctaa aggccccgtc ccccttgcca
     3421 agggggacat aaaaagcaaa acaggaattt tgtccccctt tgtttaaggg ggacgccacg
     3481 ccaaaggcat ggcagggggt agtgtccgcg ccggtggctg aaaactggtg cggaactggc
     3541 gggattgatt tttgggggta aaaaggtaac attttcctac cagatgttaa aaaactacgt
     3601 ttcgagagtc ttcaaggtca gccagcgggg agacgccaga gaggaaagct attattccgc
     3661 ccttgaaagt ctgttaaggg actatgccga atcggtcggc aaaaggggaa ctgacgttac
     3721 caccctgccc aagaaaaccg aaggaggaaa tcctgatttt cgggtgtggg acgggaaaga
     3781 gaaaatcgtc ggttatatcg aagccaagga tccttccgtt gcccatttag accagattga
     3841 aaccagcgag cagttgaagc gttatctggg tattttcccc aaccttatcc tgaccaattt
     3901 ccacgaattc cggctttatc gaaacggcga tttaatagat caggtgctta ttgcacggcc
     3961 cgtcatcggt cggaaattga aagtcgtccc gccggttgaa cgggaagaaa aattccttca
     4021 actactggag aagttcttct ctttttctct tccggcaatc accgatgcca agaccctggc
     4081 agaggaactg gcgaaaagga caaggttttt gcgggacgag attatttccg aggaattaaa
     4141 agcggggaag ggacgagttg ccgggtttta tgaggcattc aaaaagtact taatcggcgg
     4201 gttgaccaaa gaggattttg ccgacctgta ttcgcagacc attacttacg gcctttttgc
     4261 ttcacgcacc cgttcggaaa acgggttcaa ccgccggttg gcctactata acattccagc
     4321 aactattggg atcctgcggg atgtttttaa gtacatttct ctggaagatc ctccccagca
     4381 aatggaatgg attattgacg acatttcaca aattctggca aatacaaagg tcaatgacat
     4441 ccttcaccgg tattttcacg agggaaaggg caaagacccg atcattcatt tttatgaaac
     4501 ctttctgacg gaatacgacc cggatacgag ggaacggcgc ggggtttatt acacgccgga
     4561 gccggtggtc tcctatatcg tccgttcttt acacaaaatt ttgaaagagg aattcaacag
     4621 accggacgga tttgccagca cctccgttac cgttttggac cccgccgctg gaacgctgac
     4681 ctttttggcc gaagcggcta aggtggccgt ggatgaattt gtcgaaaaat acggtgaagg
     4741 cggtaaacag aatctaatca aagaacatat cctgaaaaat ttctatgcct ttgaactgat
     4801 gatggctccc tatgctatcg gtcacttaaa gatgtctttc cttcttgaag aattaggata
     4861 caggttgcag aaggatga
//
LOCUS       DBNGZA010000503         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_313373, whole genome shotgun sequence.
ACCESSION   DBNGZA010000503 DBNGZA010000000
VERSION     DBNGZA010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_313373"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..920
                     /gene="mscL"
                     /locus_tag="AC16FU_10725"
     CDS             486..920
                     /gene="mscL"
                     /locus_tag="AC16FU_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR2994881.1"
                     /translation="MLKEFKEFAMRGNVLDMAIGIIIGAAFGKIVTSLVNDVIMPPLG
                     LVIGKVDFSNLFVNLSGQHYATLAEAKAAGASTIGYGVFLNTVIDFLIVAFVIFLLIK
                     QVNRMKRQPAAVPTTKECPHCLSAIALKATRCAYCTADLKVA"
     gene            1199..1651
                     /locus_tag="AC16FU_10730"
     CDS             1199..1651
                     /locus_tag="AC16FU_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR2994882.1"
                     /translation="MEAEVYPNDRVAQLISANFLPVKIHVKEQPQTFGRFNAKWTPTL
                     IVLDSDGTERHRIEGFLPLDDFLAQLELGLAKLSFEHLQYGEAEKRFRSIQQKYAASG
                     AAPEAAYWAGVSAYKATNKPEPLGDTAKLLKVKYPDSEWTRKASVWIH"
     gene            complement(1685..2167)
                     /locus_tag="AC16FU_10735"
     CDS             complement(1685..2167)
                     /locus_tag="AC16FU_10735"
                     /inference="COORDINATES: protein motif:HMM:NF018425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR2994883.1"
                     /translation="MRREFKPKFDRWLVALIFGPFLVIGIVLVIVSWAGTKSHPAQAG
                     EMLAPLFILAGVAIILISLMRSTRYILTASELVVKIGPIHFKFPYGTIQAVETGGAEK
                     LWSHGGALRMAFSNDNLVIKVAGRFIRSVVVSPEEKTEFLLLLKTYCPALAGESPARW
                     "
BASE COUNT          505 a          595 c          674 g          447 t
ORIGIN      
        1 taagttggga tggaactgat aagcggggta aagaagttgc cagcggaatt tatttttatc
       61 ggttaagggc gggggatttc agcgagacga agagaatggt gttgctcaaa taagaccaga
      121 cccctccctc gccctcccct caaggggagg gaaaatgata gagaaaggtg tcgtcgggta
      181 ggggcgaatc tttacctgtc ctgagcgaag tcgaaggaga ttcgtccgga gggtgtgaac
      241 ggttaactga ctgagaaccg gccttaaggc cggttctcag aaacccaaaa gaacatacct
      301 caggcgggtg tccctgaagg ttgcccccac atgcttgaag cgatgagagt agaatttcga
      361 ccccttcctt tttccctccc ctcggagggg agggtggggt ggggtcaggc gcatagggtt
      421 gcgggcgaac acgggggttc gcccctacaa actctcgcgg gttcttaaga aaggaggatc
      481 aagaaatgct gaaagagttc aaggagtttg ccatgcgcgg gaacgtgctt gatatggcaa
      541 tcgggatcat catcggggcg gcgttcggga aaatcgttac gtctcttgtc aatgacgtca
      601 tcatgccgcc gttgggcctc gtcatcggca aggttgattt ttccaacctg ttcgtcaatc
      661 tctccgggca gcactacgcc acgctggcgg aggccaaggc cgccggcgcc tcgaccatcg
      721 gctacggcgt attccttaac acggtgatcg acttcctgat cgtggctttc gtcattttcc
      781 ttttgatcaa acaggtcaac cggatgaaaa ggcagccggc ggcggtgccg acgaccaagg
      841 agtgtccgca ctgcctgtcc gccattgcgc tcaaggcgac gcgctgtgcg tactgcacgg
      901 cggacttgaa ggtggcgtaa tgcatttacg ctgacccctc cccatacggg ccgtatggcc
      961 tgccctcctc ggtacgagtc ttcgaccctc aaggggaggg aaatgagagc cccgacaaaa
     1021 cctgcctgta taaacaaaac ctattcggta gcgtatatac tgcgaacaga ggaggtttgt
     1081 gatggccgcg attaactggt ccaaagacgt cgattcaacg ctcaccgaag cgaaaaaagc
     1141 gcagaagccg gtgcttctgg atttcagcgc cgcgccgatg tgaagcgggt gcgctcggct
     1201 ggaagccgag gtctacccca acgaccgggt ggcgcagttg atttcagcga atttcctgcc
     1261 ggtcaaaatc cacgtcaagg agcaacccca gaccttcgga cggttcaacg ccaagtggac
     1321 gccgactcta attgtgctcg actccgacgg caccgaaagg caccggatcg aaggatttct
     1381 gccgctggac gattttttgg cgcagttgga attagggctg gcaaaactgt cgttcgagca
     1441 ccttcaatac ggcgaggcgg agaagcgctt ccgctcgatt cagcaaaagt atgccgcaag
     1501 cggtgcggcg ccggaagcgg cctactgggc gggcgtgtcg gcctacaaag cgacgaataa
     1561 accggagccg ttaggcgata ccgccaagct cctcaaagtg aagtatcccg acagcgaatg
     1621 gacgcgcaaa gcgtcggtct ggattcattg aacgaagcgg agcgctaatt tccggagagc
     1681 ctctctacca acgggcggga gactctccgg caagggcggg gcagtacgtt ttcaggagca
     1741 aaagaaattc agttttttcc tccgggctca ccaccaccga gcggatgaac cggcccgcca
     1801 ctttaatcac gaggttgtca ttcgaaaagg ccatccgcag cgctccgccg tggctccaga
     1861 gtttttccgc gccgccggtt tcgacggcct gaatggtgcc gtacgggaat ttgaagtgga
     1921 tcggcccgat cttcacgacc agttcgctcg cggtcagaat gtaacgggta gagcgcatga
     1981 gggaaatgag gatgatggcg actccggcca ggatgaaaag cggcgcgagc atctcccccg
     2041 cttgggcggg gtggcttttg gtgccggccc aggaaacaat caccagaaca attccaatca
     2101 ccagaaacgg gccgaagatc aaggccacga gccagcggtc gaatttcggt ttgaattcac
     2161 ggcgcatatc tgagttccgg tttgcttaag gacgcgccgc ccctcagctt tgttcggggc
     2221 g
//
LOCUS       DBNGZA010000504         2128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2329677, whole genome shotgun sequence.
ACCESSION   DBNGZA010000504 DBNGZA010000000
VERSION     DBNGZA010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2329677"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="AC16FU_10740"
     CDS             complement(<1..1344)
                     /locus_tag="AC16FU_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HIR2994884.1"
                     /translation="MKKLLPQIVFSSLLVWVSVAVAQIGGATPIAADAEFCLRIEKKT
                     YFLGWHGAVVCINNEREASDPDLANGVGVSGFNFLLAYDPVCLTLQKVEPGQFLKDCQ
                     WEFFTYRFGPLGNCGGGCPSEFIRVVALADLNDARQYHPPPACSRRNSGQWVCLFFEL
                     TRDRTFENICCPIRWWFRDCTDNTISDSTGNVLWTVDSVYNAEGVPLDLATEYGSGVD
                     VSNCVSSGGQTKKRVVLKDGEICIPAEPAVDMPGDLNLNGLRFEIGDAVLFENYLLQG
                     LSALSPDPQRRQAQIASADMNRDRQINLADLVYLLCVILGDCQFPPPPQKPAVGVSAG
                     VTIDLIPQGREVAVLSDNRAVDLGGLLLVLKVNDTVADVSLTGKASGMTLRYLQAGDQ
                     LRILVDAHERGRYIAAGGGEILKITGVAAAEYVEAQAADRTGHPVTIGLAKPPLPV"
     gene            complement(1464..>2128)
                     /locus_tag="AC16FU_10745"
     CDS             complement(1464..>2128)
                     /locus_tag="AC16FU_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR2994885.1"
                     /translation="GDAQPFPKPAGGASPDVTIDFAERGGELFVASSNRTIDLGGLLL
                     VLKVNGQVKDVVLTGRAGEMGVRFAQTGDQLRILIDTYERGKIIAAGTGEILKITGVR
                     SAEFVEAQASNRGGYPVTIELAKSPVPTQFSLFQNRPNPFNPTTRITLALPVASDYRL
                     VIYNVAGQVVRTFAGQSEAGEVNITWDGTDENGNKVASGIYFYRGAAGTFSATKKMVL
                     IK"
BASE COUNT          438 a          659 c          590 g          441 t
ORIGIN      
        1 aacgggcaaa ggcggcttgg ccaaaccaat cgtcacaggg tggccggttc gatccgccgc
       61 ctgggcttcc acatactcag ccgccgccac gccggtgatt tttagaattt ctccgcctcc
      121 ggcggcgatg tatctcccgc gctcgtgggc atcgacgagg atgcgcagct ggtcgcccgc
      181 ctgcaggtag cgcaacgtca tcccgctcgc ttttccggtc agagaaacgt cggccactgt
      241 gtcgttgacc ttcaaaacga ggaggagtcc gcccaagtca actgcccggt tgtcggagag
      301 tacggccact tccctcccct gcgggatcaa gtcaatcgtt accccggctg agacgcccac
      361 cgccggtttc tggggaggcg gaggaaattg gcaatcgccg agaatgacgc acaatagata
      421 aacgagatcg gccaagttaa tctgccggtc gcggttcatg tctgcagaag caatctgtgc
      481 ctgccgcctt tgcggatcgg gtgagagcgc gcttaaccct tgcagcaagt agttctcgaa
      541 gaggacggcg tcgccgattt caaagcgcag gccgttcaag tttaagtcac ccggcatgtc
      601 gacggcaggc tcagcgggga tgcaaatctc tccgtctttc agtaccacgc gctttttggt
      661 ttgcccgccc gaggaaacac agttggacac atccacaccg ctgccatact cggtcgccag
      721 atcaagcggg acgccttcag cattgtaaac cgagtcgacc gtccagagca cgttcccggt
      781 cgagtctgaa atcgtgttgt ccgtgcaatc ccggaaccac cagcggatcg ggcaacagat
      841 gttctcgaag gtccggtcgc gggttaactc aaagaaaaga cacacccatt ggcctgagtt
      901 ccgccgcgaa catgccggcg gcggatgata ctgccgggcg tcattgaggt cggcaagagc
      961 aaccacccgg atgaattcgg aggggcaacc cccgccgcaa ttccccagcg gcccgaaccg
     1021 gtaggtgaaa aattcccact ggcagtcctt caggaactgt cccggctcca ctttctgtaa
     1081 ggtcaaacaa accgggtcgt aggccaggag aaagttgaaa ccgctcactc ccaccccgtt
     1141 ggccagatcg ggatcggagg cctcccgctc gttattgata cagaccactg cgccgtgcca
     1201 gccgaggaag taggtcttct tctcgatccg gagacaaaac tctgcatccg ccgcaatcgg
     1261 cgtcgctccc ccaatctgcg ccaccgcgac actcacccac acgagcaaag aagagaatac
     1321 tatttgtgga aggagctttt tcaccgcttt ttccaacctc tgatccgctt ccccggttct
     1381 cgtacttcct ccccaagaaa aatccccctt gcggcagggg gatttttctt attgttaacc
     1441 tacagaaccc gcccgcggcg gggttattta atgagtacca tctttttggt ggcggagaag
     1501 gtcccggccg ctcctctgta gaagtagata cccgaggcca ccttgttgcc gttctcatcg
     1561 gtaccatccc aggtaatatt cacctcaccc gcctcgctct gtccggcaaa ggttctcacc
     1621 acctggcccg ccacgttgta gatcacgagc ctgtagtcgc tggctaccgg tagggcgagg
     1681 gtgatacgcg tcgtcgggtt gaatgggttg ggccggttct ggaagagtga gaattgagtt
     1741 ggcaccggtg atttggccag ttcgatcgtt acggggtacc cgccccggtt cgacgcttga
     1801 gcttccacaa attcagccga acgaactccc gtgatcttta aaatctcgcc tgttcctgcg
     1861 gcaatgattt tgccccgttc gtaggtgtcg atcaggatgc gcagctggtc gcccgtttgg
     1921 gcaaagcgca cgcccatctc gcccgctctc ccggttagga ccacatcctt gacctgaccg
     1981 ttcaccttca aaacgaggag taatccgccc agatcgatgg tgcggttgga actggcaacg
     2041 aataactcgc ccccccgctc ggcaaagtca atggtgacat caggagatgc gccgcccgcc
     2101 ggtttgggga agggctgggc atcgcccg
//
LOCUS       DBNGZA010000505         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1483253, whole genome shotgun sequence.
ACCESSION   DBNGZA010000505 DBNGZA010000000
VERSION     DBNGZA010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1483253"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16FU_10750"
     CDS             <1..394
                     /locus_tag="AC16FU_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="High-affinity nickel transporter"
                     /protein_id="HIR2994886.1"
                     /translation="PIDWLSSWSERLVGLVLVGIGIWGFRRAFAKRVHSHVHPRAAVA
                     VGTIHGLAGSSHLFGILPALAFPSKTEAVAYLVAFGAGTITAMASFSSVIGWTATKFA
                     VERAKVYRGLLCGFSAAAIVVGGVWLVL"
     gene            424..807
                     /locus_tag="AC16FU_10755"
     CDS             424..807
                     /locus_tag="AC16FU_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994887.1"
                     /translation="MHHFWRFFGFWFLGGPALVTLFLVSCSGEGKVTLLNDTFFSPLQ
                     YQIVAVASGEPAAQGTLAADQQVTRTIKEGTYRILFRYEGQTDDFCSEDTVTVSGGSG
                     CSTGPELRTTPVLGDCGGGHGGHGG"
     gene            804..>2302
                     /locus_tag="AC16FU_10760"
     CDS             804..>2302
                     /locus_tag="AC16FU_10760"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HIR2994888.1"
                     /translation="MRNSFSKEHSTGHPPRDAGAVHSAQKMRIGSALILLAVVTFSAC
                     SDKKIVSPPPEPPAVFSRDVLPIFNRSCNRSGCHISGDTLVNPSRFFLDSFDSLRFSA
                     KTLLAEKPDHPVIIPGLGVESHLVLHLLGIDTPLMPLGGPPLPRNEIKKVIQWINEGA
                     RGDNGEPFYDPAGKIYVANSGQGTVTVIDPDLNLILKTIPVARNPNNAAAEFAHHFAW
                     NFTDNFLYLTMAGQLGELVKIDPGSDQIVARLAVGVAPTDVRISPDGRFAYVANHDLN
                     PFLPRSTVTKINLTTFTVAGTITVGKAPHGLRMSQDGRFMLACNYLTDDVTFIYLNTN
                     GDADSTFTLKLGSAPKIFRPYGIGFSPDDSLAFVSCQQSNEVRVVDLKNRLIVDSIPV
                     GFEPFMVERTPDNRFLYVPCRQSDAVYIIRLSDRTVVKSVGVGFRAHGVDFSPDGLRA
                     YITCEGDHINPSFIYVINAVTQRIIGPIEVGVFPNGVLVSPLASTSQFAR"
BASE COUNT          439 a          669 c          665 g          529 t
ORIGIN      
        1 accgattgat tggctttctt cctggagtga acgattggtt ggtctggtcc tggttggcat
       61 aggaatctgg gggttccgca gggcgtttgc aaaacgggta cactcccacg ttcacccgcg
      121 ggcagcggtc gccgtcggga caattcacgg cctggccggg agttctcacc tcttcggcat
      181 ccttccggca cttgcctttc cctcaaaaac cgaggcggtg gcgtacctgg ttgcattcgg
      241 cgcaggaacg attacggcga tggcctcatt ttcttcggtg attggttgga cagcgaccaa
      301 attcgcggtt gaacgtgcga aagtctaccg cgggcttttg tgtggattct cggctgcggc
      361 catcgtggtc ggcggcgtgt ggcttgtttt gtagaatgga gaaggcgtaa attgaggcaa
      421 tccttgcacc atttctggcg ttttttcggc ttctggtttc tggggggacc ggcgttggtt
      481 actctctttt tggtcagctg ctccggcgaa ggaaaagtga cgcttttgaa tgacactttc
      541 ttttctccgc tccaatatca aattgtggct gtagcctctg gtgaaccggc ggcgcaaggg
      601 acgctggcgg cagaccagca ggtaacccgg acgatcaaag agggaaccta ccggattctt
      661 ttccgctatg aaggacagac tgatgatttc tgctcggaag acacggtgac ggtttccggc
      721 ggctccggct gcagcaccgg gccggaactg agaacgaccc ccgtcttggg tgactgcggg
      781 ggcgggcacg gtggacacgg cggatgagaa attcattttc caaggagcac agcacaggtc
      841 acccgccgcg tgacgcagga gcagtccact cggcgcaaaa gatgcggatc ggctctgctc
      901 tgatcctgtt ggcggtggtg acattttccg cctgtagtga caaaaaaatt gtttccccac
      961 cgccggagcc gccggccgtt ttctctcgtg acgttttgcc aattttcaac cgttcctgca
     1021 atcgatccgg ctgccacatc tccggggata cgctggtcaa tccatcgcgg tttttcctcg
     1081 actcctttga cagcctgcgg ttctccgcca aaacgctctt ggcggagaaa ccggatcatc
     1141 cggttatcat ccccggcctg ggcgtggaaa gccacctcgt tttgcacctg ctgggtatcg
     1201 atacgccctt gatgcccttg ggcgggccgc ctttgccgcg caatgaaatc aagaaggtca
     1261 tccagtggat taacgaaggc gcgcggggtg ataacggaga gcccttctac gatccggcag
     1321 gaaaaatcta cgtggccaac agcggccagg gaacggtgac ggtcatagac ccggacttaa
     1381 accttatcct gaaaactatc cccgtcgcca gaaatccgaa caatgctgcg gctgagttcg
     1441 cccaccactt tgcctggaac tttaccgaca acttcctcta cctcaccatg gcagggcagt
     1501 tgggcgagtt ggtgaaaatt gatccgggga gcgaccagat agtggcgcgc cttgccgtgg
     1561 gcgtggcccc gaccgatgtc cgtatctcac cggacgggcg ctttgcctac gttgccaacc
     1621 atgacctgaa tccgtttctc ccccgctcga cggtgaccaa gattaatctg acaactttca
     1681 cggtcgccgg caccatcact gtaggcaagg cgccgcacgg cttgcggatg tctcaggatg
     1741 gccggttcat gctggcctgc aattatttga ccgatgatgt cacgttcatc tacctgaaca
     1801 ccaacggcga cgccgattcc actttcactc tcaagctggg ctcggccccg aagatcttcc
     1861 ggccctacgg cattggattt tcgccggacg acagcctggc gttcgtctct tgccagcagt
     1921 cgaacgaggt gcgggtggtt gatcttaaaa accgcctcat cgttgactcc attccggtgg
     1981 ggttcgagcc attcatggtt gaaagaacgc ccgataaccg attcctgtac gtcccctgcc
     2041 gacagagtga cgcggtgtac atcatccgcc tctccgaccg cacggtggtc aaaagcgtgg
     2101 gggtcggctt ccgggctcac ggggtcgatt tcagtcccga cggcctccgg gcctacatca
     2161 cctgcgaggg ggatcacatc aacccctctt tcatctacgt catcaacgcc gtcacccaaa
     2221 ggatcatcgg gccgatcgaa gtaggggtgt tccccaacgg ggttttggtc agcccgctgg
     2281 cgtctacgag tcaattcgcg cg
//
LOCUS       DBNGZA010000506         8432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2329928, whole genome shotgun sequence.
ACCESSION   DBNGZA010000506 DBNGZA010000000
VERSION     DBNGZA010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2329928"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="AC16FU_10765"
     CDS             complement(<1..731)
                     /locus_tag="AC16FU_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994889.1"
                     /translation="MFTRYHIKTKVAPPRSPQPSKFVVKVDRLKFVRMIAGELKESSS
                     LSEMKGTIKSRPCLYGVYSRPVGGLAPKSSSCVACYRCVIENPGMVRILPNPDLIEKA
                     QQSGLTPKQISTLWYEAATGRVPVKGAGYNGPFSGPGFDAIWTDMSEIVRPTRDGIHG
                     REYISTAVEIGSRPAFVQFDNPGVSGSDNLVHLPIPMLFDATAIPLPYFQRAAAATAR
                     QLATLVFVPAAAAGLPGDGRNIIPLV"
     gene            complement(903..1940)
                     /locus_tag="AC16FU_10770"
     CDS             complement(903..1940)
                     /locus_tag="AC16FU_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HIR2994890.1"
                     /translation="MKTVAASRVTKLLPFSSDARLREAYVNTFGDVRFGLVLEELDHV
                     AGEAAHKHIGRNDLWVVTAAVDRIDLIHPVRIAQDLRFTAQVNYVGRTSLEVGIDIES
                     RVEETWLTAAHAFFTMVAVNQNFVPTPIDPIVPETPEEKQVMEEAKKRKVEYQQSKIL
                     SLERHPPTEEESRILHDLNPASKQSRAVIPMSVTRLENTLFMHPQDKNKFGKVFGGYV
                     MRTAYELAWSAAYKFCRERPLIASVDRINFEKPVEIGALVSFQALVSFVGRTSMGVEV
                     VVKVINPKKEEEIVTHSGYFTLICMDQNRKPIPAPKVIPQNDEEALKYLEGCRRYRRN
                     RDLRTKQKHGA"
     gene            2188..2952
                     /locus_tag="AC16FU_10775"
     CDS             2188..2952
                     /locus_tag="AC16FU_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994891.1"
                     /translation="MPLYEFLTKHSRAISATAGLIVIGGFAYGIFKLFFESADLEVIV
                     TVNDANLPTSIASKYSSSMNWFVDRFPKKEVSQDVKKALSDVLEVSSFLTETNSKATF
                     EIDNNSNKTLKNIDIRIKNVSKLTAWGVTGNSLQTIEATEIMRAMRTDPQNGIITFGG
                     IEKIPPQSSLIITIWGETRQYLIDDPISVTYDGGSGKVVRTSTVRGFASFIYENTTYL
                     LILLALVNIGAFFAVIDAVSKKMKKVQQSEQAAAPS"
     gene            3494..4246
                     /locus_tag="AC16FU_10780"
     CDS             3494..4246
                     /locus_tag="AC16FU_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994892.1"
                     /translation="MTTKARGQFIRRHVGGFLLLGSLICFLLLLINWRLELQKVFSDR
                     TFAHAQLNPIERTGLTPVFQRFSKTGYPVFSLITKRSDYLIPYPTEEKHDMLEVLLYI
                     PERQFDNKELHQRIVSEVEITRRLYNHLFRANIYQSRLTLRRTGVFGSFIFTSEYKYI
                     IAPTQDRKIVFIGDIPAKVKNQILENLSLHKLNGLWYDSLWFTAPYPGFFEAKPEKQF
                     IVEFSSGLMPSVFGSGFAFLISPDGSLAMRED"
     gene            4397..4627
                     /locus_tag="AC16FU_10785"
     CDS             4397..4627
                     /locus_tag="AC16FU_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2994893.1"
                     /translation="MAPKARKADPNKVVIFKPDLCGHCGACVAVCGFDSLVLYRTGIA
                     VDMNTCILCDACVRTCPTFALEITNGKLQFSH"
     gene            4602..5801
                     /locus_tag="AC16FU_10790"
     CDS             4602..5801
                     /locus_tag="AC16FU_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR2994894.1"
                     /translation="MANYNSRTDYDIIVVGAGPGGSLAAKSAAEKGARVLLMEKHAAV
                     GYPLCCAEAISTTGLTACVPLNPRWIASKVERVVLIGPGGAKLFIQHPEAGFVLDRKI
                     FDRDLASYAGVAGAEVKVNCQAVGLIPNNGTGYAGVRAVENDGEKEYKAKVIIGADGV
                     ESQVGRWAKMNTTLPLGGIDACAQYLLGGLDIPEDLLEFYFGKNVAPGGYIWVFPKGN
                     GTANVGVGIMPSQVKGKTAKEFLDDFVSRRFPNAKILELMVGGVPMFRGRKFMQKDNI
                     LLVGDAARLVDSLSGGGIANAMLSGQVAGEVAAEFVQNGYRSFDYLKEYPQRFMKTKG
                     RELRYYLYARHIFTKMKDDDLDFTLDFLNPLFDGQPVSALEPIKTIKQVLKKRPSLLL
                     YARHIRW"
     gene            6032..7609
                     /locus_tag="AC16FU_10795"
     CDS             6032..7609
                     /locus_tag="AC16FU_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR2994895.1"
                     /translation="MSLSHIVHLWLCPGWSVQPRKDLPTVGLVLLSFFLVFSSASGQG
                     SLLYQKDGTAAGIQFGQSVAVAGDIDGDGKADFIVGASLASPGGLSHAGSAFIYSGAT
                     GALLYQKNGLNSNDFLGWWVAGAGDVNGDGAADFILGAPFADSGGAIDAGSAFVYSGA
                     TGALLYQKNGAVSSDILGSSVAGAGDLNGDGKADFMVGASLASPAGIPYAGSVFVYSG
                     ATGTLLYQKNGPDSNSFLGSSVAGIGDANADGRGDFIIGAPGAGTGKGSVFVYSGSNG
                     ALLYQKNGVVGSDGISLGDQLGSAVAGAGDVNGDGRADFMGGAPGADTGKGSVFVYSG
                     SDGVLLYQKNGDVASDGINLGDQLGFAVTRTEDVNGDGRADFMSGSPGADGGGLTDAG
                     SAYVYSGATGALLFQTNGGAAGDLLGYSAAGGGDLDGNGRPDLVIGVPNADPGGLTDA
                     GSALVYCSGPLRGDVNNDAVTDILDLVALVQDVVFEIPLPNPLAGDANCDGVRDIQDL
                     VLLVLYVVFGSPTPCCL"
     gene            complement(7845..8279)
                     /locus_tag="AC16FU_10800"
     CDS             complement(7845..8279)
                     /locus_tag="AC16FU_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2994896.1"
                     /translation="MLFTKAGEYGLFGLMYLAQKPNGRVTRLADISKAQKVPEKFLAK
                     IVQVLVRKGFVHSHRGTKGGFSLARPANSMTLGEILTAVQGPPAVAECFRPGHECGLY
                     SSCNIRVALEGVQEQLNRSLARFTLADLVPRGSAVAVGARKG"
BASE COUNT         1837 a         2311 c         2253 g         2031 t
ORIGIN      
        1 accaaaggaa tgatgttcct tccgtcgccc ggaagacccg ctgccgccgc cgggacgaaa
       61 accagagtcg ccaactgacg cgccgttgcg gcagccgccc gctggaagta gggcagcgga
      121 atggccgtgg cgtcgaaaag cattgggatc ggaaggtgca cgagattgtc ggagcctgac
      181 actcccggat tgtcaaattg gacgaacgct ggtcgagaac caatctccac tgccgtggag
      241 atgtactctc gtccgtggat gccgtcccgc gtcgggcgga cgatttccga catgtcggtc
      301 cagatggcgt cgaagccggg gccggagaaa ggcccgttgt agccggctcc cttcaccggc
      361 acgcggccgg tcgccgcctc gtaccagagg gtggagattt gtttgggcgt caggccggat
      421 tgctgggctt tttctatcaa atccggattc ggcaggatgc gcaccatccc cggattttcg
      481 atgacgcagc ggtagcaggc cacgcaggaa ctgcttttgg gggccaagcc gccgaccggc
      541 cggctgtaga cgccgtacag gcagggccgg cttttgatcg tccccttcat ttccgaaagc
      601 gaagacgact ctttcagttc cccggcgatc atccggacga acttcagccg gtcaaccttc
      661 accacgaact ttgacggctg cggtgaccgg ggtggagcta ctttcgtctt gatatggtag
      721 cgggtaaaca tgcttgcttt ggctttaact tttatagttt gcgatccgac ctgcagttcg
      781 gatagacttc gccttcatct tctcaaaaat tgtcacgtaa cctgaagtac aacgctgaac
      841 cctaccgcag catacatcgg aattgaacgg aacgcaagaa aaattctcga agacgttctt
      901 tttcaggccc cgtgcttctg tttagttcgc aagtctcggt tccttcgata ccgtcggcag
      961 ccctccaaat atttcagcgc ctcttcgtca ttctgcggaa tgactttcgg cgccggaatc
     1021 ggcttccggt tttgatccat gcagatcaaa gtgaagtacc cgctgtgggt tacaatttcc
     1081 tcttcttttt tgggattgat caccttgacc accacctcca cccccatcga ggtgcgcccg
     1141 acaaaagaga ccagcgcctg gaaactgacc aaggccccga tttcgaccgg cttttcgaag
     1201 ttgatgcgat cgacggatgc gatgagtggc cgttcgcggc aaaacttgta ggccgccgac
     1261 caggccagtt cgtaggcggt gcgcatcacg tatcccccga acactttccc gaatttgttc
     1321 ttgtcctgcg gatgcatgaa gagggtgttc tccaggcgcg tcacggacat cggaatgacc
     1381 gcacggctct gttttgaggc cgggttaaga tcgtgcagta tccggctttc ttcttccgtc
     1441 ggcgggtggc gttcgagaga gagtattttg ctctgctgat attctacttt gcgtttcttc
     1501 gcctcttcca tcacctgctt ttcttccgga gtttcgggga cgattgggtc gatgggtgtc
     1561 gggacgaaat tctgattcac cgccaccatc gtaaaaaaag cgtgtgcggc ggttagccag
     1621 gtttcttcga cgcgggactc gatgtcgatg cccacttcca gcgacgtgcg gccgacgtag
     1681 ttgacctgcg cggtgaaccg caaatcctgg gcgatgcgta ccggatgaat caggtcaatc
     1741 ctatccactg ctgcggtgac gacccagagg tcgttgcggc cgatgtgctt gtgagccgcc
     1801 tcccccgcca catggtcgag ttcttccaac accagcccga agcgcacgtc gccgaaggtg
     1861 ttgacgtacg cctcgcgcag gcgtgcgtcg gaagaaaagg gcagaagttt ggtgacccgg
     1921 gaggcggcaa cggttttcat cgctcgaccg gataataccc ccaactcgta agtggacgca
     1981 aggaagaact tgaaccttca aggaatactt gattttggtc ataccatgcc ttaacttttc
     2041 cgggtgtgaa tattcacggg cggggcataa cgacccaacg cacctttata cgaaattcct
     2101 atatggaata gcaaaacctt aagcacgcca ggcatcagat gagatttgca caaaggaatg
     2161 ttcgtcaaac actaattcgg acggtatatg ccactatatg aattcctaac aaagcattcc
     2221 agggcaattt cagcaacagc tggtctcatc gtcataggcg gttttgctta tggcattttc
     2281 aaattgtttt ttgaatcagc ggatcttgag gtgatagtaa cggtaaacga tgctaacctg
     2341 ccaacctcaa tagcctcaaa gtactcgtca agtatgaact ggttcgtcga ccgcttcccg
     2401 aagaaggaag tcagtcaaga tgtcaaaaag gcactcagtg atgtattgga agtgtcaagt
     2461 ttcctaactg aaacgaacag caaagcgaca tttgagattg acaacaactc aaacaagacg
     2521 ctaaagaaca tagatatccg gatcaagaac gtcagcaaac ttacagcatg gggagtaact
     2581 ggtaattcgc tgcaaaccat cgaggctaca gaaataatga gggcaatgcg gacagaccct
     2641 caaaatggga ttataacatt tggaggtata gagaaaattc cacctcaatc atcgctcatt
     2701 attacgatct ggggtgaaac tcgtcaatat ttgattgacg atccaattag cgtcacctat
     2761 gacggtgggt cgggcaaagt tgtccgaacg tcaaccgtaa gaggattcgc atcctttatc
     2821 tacgaaaaca cgacctacct tcttattctt ttagccttag taaacatcgg agcttttttc
     2881 gctgtgattg atgcggtcag caaaaaaatg aaaaaggtac agcaaagtga gcaagctgcg
     2941 gcaccgagct aagtccgttc tgaatgcagt tgctcgatgg ttgacgcaac tcagagttaa
     3001 ctacgaattc ctgtttatcc gtcggtgtct cgctttcttc attgatgctg caatcctact
     3061 cgctttctcc ctcgctgtgg cctttgtaaa tttctttgct ggttacaatg tcctcctgcc
     3121 agagaccgct aaaatcgttc tgccgtccta ctttctgctg agtcgattca tatctaacgg
     3181 gagaacgatc ggcatgagaa cgtttagact tcattttgcc tttgaagtta tgcaatttag
     3241 gcgcgttcga cttttgtata ggtttttgct aagcgtagca ctattgctcc tcccagtcga
     3301 attcattccc attgttgaat atctgctccc taatggcttc agcaaattcc atttgactct
     3361 tctctctttt ggcatgctga gttcaatccc gatatacagc tttctaatga ttatgttctc
     3421 caatggttca gcatctctcg tggatcgtct ctcgaaatgc caaatctcct ctcgatcaac
     3481 gccatcagat ggtttgacta ctaaggctcg aggccaattt attagaaggc acgtcggtgg
     3541 ttttctactg ctaggatctc tgatatgttt tcttcttttg ctcatcaact ggcggctcga
     3601 attgcaaaaa gtcttttctg acagaacttt tgcacatgcc cagctaaatc caattgagag
     3661 aactggcctc acaccagtgt ttcagcgatt ctccaaaaca ggttacccag tgttttcatt
     3721 gatcacgaag cgaagcgatt atctgattcc atatcccacg gaggaaaagc acgacatgct
     3781 tgaggtgttg ttatatatcc ccgaaaggca attcgacaac aaagagctcc atcaacggat
     3841 tgtttctgaa gttgaaatta cgaggagatt gtacaaccac cttttccgtg caaacatcta
     3901 tcagtcacga ttaactcttc gtaggacagg agtctttgga tcatttattt ttacgtctga
     3961 atacaagtac attatcgcgc caacacaaga ccgcaaaatt gtattcatag gtgacattcc
     4021 agcaaaagta aaaaaccaga ttcttgagaa cttatcctta cataaactca acggtctatg
     4081 gtacgactcc ttgtggttca ccgcaccgta tcccggtttt tttgaggcta aaccggaaaa
     4141 acagtttatt gttgaatttt ctagtggact tatgccatcc gtttttggtt ccggtttcgc
     4201 ttttcttatc tctccggatg ggtctttagc gatgcgcgag gattaacaaa ttagcatttg
     4261 atgtaacgag aaagacagct aagcagttcg ctccgcttca accggttaag cgtagtaaca
     4321 actccctatc cgaatgcaac ccgctgttga cagcttgtcg cttcggactc tatgttttgc
     4381 agagacgaga gagcccatgg cgcccaaggc acggaaagcc gatccgaaca aagtcgtaat
     4441 cttcaagccc gacctctgcg gccactgcgg cgcctgcgtg gcggtctgcg ggtttgattc
     4501 cctcgttctc taccgcaccg gcatcgccgt cgatatgaac acctgcattc tctgcgacgc
     4561 ctgcgtccgc acctgcccca cctttgcgct ggaaattacg aatggcaaac tacaattctc
     4621 gcactgatta cgacatcatt gtcgtcggcg ccggtcccgg cgggtcgctc gccgcgaaaa
     4681 gtgctgcgga aaaaggcgcc cgcgtgctct tgatggaaaa gcacgccgcg gtcggctatc
     4741 ccctctgctg cgccgaagcg atcagcacca ccggcctgac tgcttgcgtc cctttgaatc
     4801 cgcgctggat agccagcaag gtcgaacggg tggtcctgat cggcccgggc ggcgcgaaat
     4861 tattcatcca gcatccggaa gccggcttcg tcctcgaccg gaagattttt gaccgcgatc
     4921 tcgcctcgta tgccggtgtg gctggcgcgg aagtgaaagt gaattgccag gcggtcggct
     4981 tgattccaaa taatggaacc ggttacgcgg gggtgcgcgc cgttgaaaac gacggcgaaa
     5041 aagagtacaa agcgaaagtg atcatcggcg ccgacggcgt ggaatcgcag gtcggccgct
     5101 gggccaagat gaatactacc ctgcccttag gcggcatcga cgcctgcgcc cagtatttgc
     5161 tgggtggact cgatattccg gaagacctgc tcgaattcta tttcgggaaa aacgtcgcgc
     5221 ccggcggcta catctgggtc tttcccaagg ggaacggcac cgccaacgtc ggcgtgggga
     5281 ttatgccctc ccaggtgaaa ggcaaaaccg ccaaagagtt tttagatgat tttgtttccc
     5341 gccgctttcc gaacgcaaag attctggaac tgatggtggg cggcgtcccg atgttccgcg
     5401 ggcggaagtt catgcagaaa gacaacatct tgctcgttgg ggacgccgcc cgtttggtcg
     5461 attccctctc cggcggcggg atcgccaacg ctatgctctc cggccaggtg gcgggcgaag
     5521 tcgctgcgga gtttgttcag aacggctacc gctcctttga ctacctgaag gagtatcccc
     5581 agagatttat gaaaacaaaa ggccgggaac tgcgctacta cctctacgcc cggcacattt
     5641 ttaccaaaat gaaagacgat gacttggact tcaccctcga ttttctcaac cccctcttcg
     5701 acggccaacc ggtctctgcc ctagagccga tcaaaaccat caagcaggtc ttaaaaaagc
     5761 gcccctccct cctcctctac gcaaggcata tccggtggta aagcatactg tcgaagaatt
     5821 catcctcacc ctacagcggg agtttggtat cccgcccgct gaccttcaga gcttgacagg
     5881 ttaatagatt ttcatataat tactttcagc gagagcgcaa ggagggggct cgcgaggaga
     5941 gttcattacg atgagcgtct ggaccggctg gtctgcatta actgtcggaa acgacccatt
     6001 cttgaggagt cgtaccgacc acggaggttc tatgtccctt tctcacatcg ttcacctgtg
     6061 gctctgtccc ggatggtcgg tacaaccccg aaaagacctg ccgaccgtcg ggctggtctt
     6121 gctctccttc ttcctcgttt tttcctccgc ttccggccag ggaagtctcc tttaccaaaa
     6181 agatggtacc gctgcgggta ttcagttcgg ccagtcggta gccgtggccg gggatattga
     6241 tggcgacggg aaggccgatt ttatagtcgg cgcctctctg gccagccctg gcgggctttc
     6301 ccatgccggc tcggctttta tctactccgg ggctaccggc gctcttctgt accagaaaaa
     6361 cggcctcaac agcaatgact ttctgggttg gtgggtggct ggggccgggg atgtgaatgg
     6421 cgatggtgca gccgatttca ttcttggcgc tccctttgcc gactcgggcg gggcgatcga
     6481 tgccggttcg gcttttgtct actccggggc taccggcgct ctcctttatc agaaaaacgg
     6541 cgcggtttcc tccgacatat tggggagttc ggtggccgga gccggagacc tgaatggcga
     6601 cggcaaagcc gattttatgg tcggcgcctc tctggctagc ccggccggga ttccgtatgc
     6661 cggctcggtt tttgtctatt ccggagccac cggtactctc ctgtaccaga aaaatggccc
     6721 cgatagcaat agcttcctgg gcagttcagt agccggaatc ggagatgcaa atgcggacgg
     6781 gagaggcgac tttatcatcg gcgctccggg agccggcacc ggcaagggtt cggtctttgt
     6841 ctattccggg agtaacggtg cgctgctcta ccagaaaaac ggcgtcgtcg gcagtgacgg
     6901 gatcagccta ggcgaccagt tgggctctgc ggtggccggg gccggcgatg tgaatggtga
     6961 tgggagagcc gactttatgg gtggcgctcc gggagccgac accgggaaag gttcggtctt
     7021 tgtctattcc gggagcgacg gcgttctact ctaccagaaa aacggcgacg tcgccagcga
     7081 cgggatcaac ctgggtgacc agttgggctt tgcggtgacc cgtacggagg atgtgaatgg
     7141 tgacgggaga gccgacttta tgagcggctc tccgggtgcc gatggcggcg gcctcactga
     7201 cgctggttct gcgtacgtct actccggcgc cacaggcgcc ctgcttttcc aaacgaacgg
     7261 cggcgccgcc ggtgaccttt tgggctattc ggcggccggg ggcggggatc tggatgggaa
     7321 tggtaggccc gatctcgtta tcggcgtacc caacgccgac ccgggcggac tgacagatgc
     7381 cggctcagcc ctggtgtact gctccggacc gctgcggggc gatgtgaaca acgatgcggt
     7441 gaccgacatt ctggaccttg tggcgttggt tcaggatgtc gtgttcgaaa tccctctgcc
     7501 caaccccctg gcaggcgatg cgaactgcga cggcgtcagg gatattcagg accttgttct
     7561 cttagtcctg tacgtcgtgt tcggcagtcc tactccctgc tgtctctaac tagggtatcg
     7621 tgagtccgct tgaggaggac cgaaccacag tccaactcct tctacaagtg accggcgggc
     7681 aagaaaacta tagaggtaaa cccacctgag agacaggttc accccttctt ctattcaaat
     7741 tgataacaac caatacccat aggttaggca tcttgcctga ccggtctccg aggcagaaag
     7801 cctcggctat ggtaacctaa tttctttcgc ctgtctcgaa ccggctaccc tttccgggcg
     7861 ccaaccgcca ccgccgagcc ccggggaacg aggtccgcca gggtgaaccg cgccagcgac
     7921 cggttcaact gctcctgaac cccttccaaa gccacccgaa tgttgcaact gctgtagagt
     7981 ccgcactcgt ggccggggcg gaagcactcg gccaccgccg ggggcccctg caccgcggtc
     8041 aggatttctc ctaaggtcat gctgttggcc gggcgcgcca gggaaaaacc gcccttggtc
     8101 ccccggtggg agtggacgaa tcccttccgc accaaaacct ggacaatctt ggccaaaaac
     8161 ttctccggca ccttctgggc tttggaaatg tcggccagcc gggtcaccct gccgttgggc
     8221 ttctgcgcca ggtacatcag gccgaacagt ccgtactcac ctgctttggt aaagagcatc
     8281 tgccacctcg cttcctttcc cgtcaaccgt gaccgcttgg ggcctgaatc ggatatcgat
     8341 attcaggtct tgtagtcgta atatagtata cgagaatcgt ccgctcctgt ataaccttat
     8401 ttttcggtcg ggaatttctc ttcgaactcg cg
//
LOCUS       DBNGZA010000507         6556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1055885, whole genome shotgun sequence.
ACCESSION   DBNGZA010000507 DBNGZA010000000
VERSION     DBNGZA010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1055885"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16FU_10805"
     CDS             complement(<1..217)
                     /locus_tag="AC16FU_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994897.1"
                     /translation="MGPVSGCCQLGVIGAGAGAGAGFFGRALRAALGLAFIRAFPIFL
                     VTLFALARLAVFRLAFFILSPPFLVSLW"
     gene            379..2865
                     /gene="leuS"
                     /locus_tag="AC16FU_10810"
     CDS             379..2865
                     /gene="leuS"
                     /locus_tag="AC16FU_10810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR2994898.1"
                     /translation="MPAQYPFKEIEAKWQKIWEEINLFRAPEPPQKKFTVIPMFAYPS
                     GDIHMGHFRNYTITDAIARFKRMQGYDVLHPFGWDAFGLPAENAAIKRGIHPREWTLN
                     NVAVSKATLKRVGILFDWEREVTTCLPDYYKWTQWLFLKLWEKGLAYRAEAPVNWCPS
                     CQTVLANEQVEQGRCWKCDSVVVKKELTQWFFKITAYAERLLKDIEALTGWPENVKTL
                     QKNWIGKSTGCFIDFPLEGSDLKLPVFTTRPDTVYGVTFLSIAPDSRLFKKIEIAGPR
                     KKEVAAYVEKAGARTEIERAAASEEKDGVFTGRFVRNPLSGEQVPLWVADYVLAGYGS
                     GVVMGVPAHDQRDFEFARKYRLPVKVVIVPPEAGAGEKLPEAERMEAAFTDYGRMTNS
                     GPFDGKVGREAIKAVIDHVEKQGWGKGAVQYKLRDWLISRQRYWGCPIPIIHCPDHGE
                     VAVPEKDLPVLLPPEVKDYIPKGRSPLADLPEFVNTVCPKCGKPAQRDVDTMDTFIDS
                     SWYFLRYADPHNTREPFDRKKANTWLPIDLYVGGIEHATGHLIYFRFFTKVLYDLGYL
                     GFEEPAARLFNHGMVLDAGGQVMSKSLGNVVTPDEVMTKEGADVSRLTMYFAAPSNRE
                     MFWSTEGVVGISRFLTRLWKLIEGRGKIDSAAVFQLVAASQINGADLKLYRKLHQTIK
                     KVTEDFETLQFNTAIAALMELLNEIPAQKPERSRVYDLTLIRIAQLLAPLAPHIAEEF
                     WQILGFSGSIFRSQWPEHDPAALVEEELTIAVQVNGRLRATVNVPVTADEGFITGQAQ
                     QHDRVKPHLKDKKVLKTVYVPRKLVNIVVQ"
     gene            3252..3842
                     /locus_tag="AC16FU_10815"
     CDS             3252..3842
                     /locus_tag="AC16FU_10815"
                     /inference="COORDINATES: protein motif:HMM:NF037752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HIR2994899.1"
                     /translation="MLRGATVLAVVMLAVIVYPSVGNSFVYKVPFTITAGEIQANGGN
                     PFPAVTIDQVDLGGLQGLSAGPCQANALISGIKITRRGNAKPLLSNVQPPGYAVAVTI
                     SRDLGLGEVALPAGEYDAWFKSDKGPCDTPKARITKKGSGAFSISTVAAVTTIDTATV
                     EPVQLPTLNQWGLILLVAVLGGIGTFVIVRRRWTAV"
     gene            4436..>6556
                     /locus_tag="AC16FU_10820"
     CDS             4436..>6556
                     /locus_tag="AC16FU_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994900.1"
                     /translation="MTLEYLTGPPVANQTPDVMVDAAVNQDGGGLMYSVGVGNWQSVQ
                     TTYGPSAGDLFIRGVFELPRRGDYGDAPDSSNGNIAAAGSGFPTQYFTKNATVIGRRG
                     PFHRDTTQFWLGDSGNAPTRERNPQNNTGDGDRDSSKINGQFYTAVVGSDTLGWFIVP
                     VSVAGSASAAVRVLNILYDQNKNGKWQNTSAAFPEWLVRNAQVFTNPGMTENYVLGPF
                     RLKNVPPGPIWSRFTFTSDSIPQALFGTGWDGSGPDTGFAAGETEDFNLLGSVSAESA
                     ALVQVAAPVTFDAFEADTGRSTREVRFINYGGKALTASEIRFFALGDPDTNFILTLVD
                     SAGQNSTNFTIPANSTVTFRYRAQFNRSSDDTVHRADRTRAFAWQLRGNSITTPLLSQ
                     AFTTFVEHVRPQFVQPVQNSFTRSACDFSFDFLVTAMDIDEEILFPLNIRYCGTCFRE
                     TVSLRAPVNLPTFFPDTVAALGTNNPGRFRWTPGVADTGVWLAKFVARDSVDKMEDTT
                     DIFISVSATSATGVIAVRHIVVTVPESTTCFSVAVRVFNADFLRTIKIPLVYDSLICL
                     KAITYDSTRLANSNILNQRFLDSTSYRNHNGLDTLVLTFFRTVGFSDLEPGCGTIANI
                     WFSTKVTDTTVNPLDTVFINSSERPFFETAGGQEVLNLQFQNEPLHPQVLLGDLDCDT
                     VRDINDIVGLINCVVFFPDPNKCTL"
BASE COUNT         1529 a         1765 c         1799 g         1463 t
ORIGIN      
        1 accaaagcga aaccaggaaa ggaggggaga ggatgaagaa agccaagcgg aaaacggcca
       61 agcgcgccag ggcgaaaaga gtaaccaaga agatggggaa ggcccggatg aaggccagac
      121 ccaaagccgc gcgtagagcc cgtccgaaaa aaccggcacc cgctccggca ccggcaccga
      181 tgacaccgag ctggcagcag ccgctgaccg ggcccacgga gacggtatcc gagccgcagc
      241 agagcttccc gatgtaggga aagcaccttt gctttcgcca gagtccacgc agggcgaccc
      301 gtacaggtcg cccttttcat tcagaactgt gttactcgga gttgcgaatt tccttctgcc
      361 tgcgtaatat cttttccgat gccggcccaa tacccgttca aggaaataga ggcgaaatgg
      421 cagaaaatct gggaagagat aaacctcttc cgggcgcctg aacctccgca gaagaagttc
      481 accgtcattc cgatgttcgc ctatccctcg ggcgacatcc acatgggtca cttccgcaac
      541 tacactatca cggacgcaat cgcgcggttc aagcggatgc aggggtacga cgtcctccat
      601 ccattcggct gggatgcctt cggcttgccg gcggaaaatg ccgccatcaa gagaggcata
      661 catccgcgag aatggaccct gaataatgtt gccgtgtcga aagcgacgct caaacgggtg
      721 gggattttat tcgactggga acgggaagtc accacctgtc tgcccgacta ctacaagtgg
      781 actcagtggc tctttttgaa actgtgggaa aaaggcctgg cctaccgcgc cgaagcgccg
      841 gtgaactggt gcccttcctg ccagaccgtt ctggccaacg aacaggtgga acagggacgc
      901 tgctggaagt gcgattcggt ggtggtcaaa aaagagctga cgcagtggtt cttcaaaatc
      961 acggcctacg ccgagcgcct gctcaaggac atcgaggccc tgaccggctg gccggaaaat
     1021 gtgaagacgt tacaaaagaa ctggatcggc aaaagtacag ggtgcttcat cgactttccg
     1081 ttggagggtt ctgacctgaa actcccggtc ttcaccaccc ggcccgacac cgtctacggc
     1141 gtcacctttt tgtccattgc gccggacagc cggctgttca aaaaaatcga gatcgccggc
     1201 ccccggaaaa aagaagtggc cgcgtacgtc gaaaaggccg gagcccgcac cgaaatcgag
     1261 cgggcggccg ccagcgaaga gaaagatggc gtatttacgg gccgcttcgt ccgtaatccg
     1321 ctttcgggtg agcaggtgcc gctctgggtc gccgactacg tcctggccgg ttacggttcc
     1381 ggcgtggtca tgggtgttcc ggcgcacgat cagcgcgact tcgagttcgc ccggaaatac
     1441 cgcttgccgg tgaaagtcgt cattgtcccg ccggaggcgg gagcagggga gaagctgccg
     1501 gaagccgagc gaatggaagc cgccttcacc gactacggcc ggatgacaaa ctccgggccg
     1561 ttcgacggca aagtggggcg cgaggcgata aaagccgtta tcgaccacgt cgaaaaacaa
     1621 gggtggggga aaggggccgt ccagtataaa ctgcgcgact ggcttatctc ccggcaacgc
     1681 tactggggct gtccgattcc gatcatccat tgtccggatc acggcgaagt agccgtgccg
     1741 gaaaaggacc tccctgttct tctgccgccc gaggtgaaag attatatacc caaaggccgc
     1801 tcgcctctgg ccgatctgcc ggaattcgtc aacaccgtct gtcccaagtg cggcaaaccg
     1861 gcccagcgcg acgtggatac gatggatacg tttatcgact cctcctggta ctttctccgc
     1921 tatgccgacc ctcacaatac cagggagcct ttcgatcgga agaaggccaa tacctggctg
     1981 cccatcgacc tctacgtcgg cggtattgag cacgctaccg gccacctgat ttactttcgg
     2041 tttttcacca aggtgctcta cgatctgggc tacctgggtt ttgaggaacc agccgcccgc
     2101 ctctttaatc acggcatggt cttggatgcc ggaggacagg taatgtccaa atctctgggg
     2161 aacgtggtta ccccggacga agtcatgact aaagaaggag cggatgtcag ccggctcacc
     2221 atgtatttcg cggcgccctc caatcgagaa atgttctggt ctaccgaagg ggtggtggga
     2281 atcagccgct ttttgacccg tttgtggaaa ctgatcgaag ggcgcggcaa gatcgattct
     2341 gcggcagtct tccaactcgt tgcggcgtcg caaattaacg gtgcagactt aaagttatat
     2401 cgaaaactgc accagacgat caaaaaagtt accgaggatt ttgagacatt gcagttcaac
     2461 acggccatcg ccgctttgat ggagctttta aacgaaattc ctgcgcaaaa accagagcgg
     2521 agccgggtat acgacctgac tcttatccgg attgcccagc tcttggcccc tctggcgccc
     2581 cacattgccg aggaattctg gcaaatactc ggtttttccg gaagtatttt ccgttcgcaa
     2641 tggcccgaac acgacccggc ggctttagtc gaggaggaac tgaccattgc cgtccaggtc
     2701 aatggccggc ttcgggcgac ggtgaacgtg ccggtaacgg ctgacgaggg gttcataacc
     2761 ggccaggctc agcaacatga ccgggtaaaa ccgcatctga aagacaagaa agtgcttaaa
     2821 acggtctacg tgccccggaa attggtcaat attgtggttc aatagaacct aaaggcgttc
     2881 agtaagttag gtacaagggg aagatggggg cctcggcgca aaatcggttc tttacataat
     2941 atatcttata ggacataaag actttgggcc aattcatgat gatttccatc cgatactttg
     3001 cccctactga caaaattttg cctgtaaaag taattttttg cttgacaagc caagattcgc
     3061 aaaagtatat tctcccctgc catttggtgg ggccggtgct ggttgcgtgc acaaccctga
     3121 ggggggttgc ttagcgaaac agcggaggtt ccttttggaa gcgggaggtt gagatatgag
     3181 acagaggttt gagctcaacc agtagcgaca gagcgaatca attcaaaatc taaacaagag
     3241 aggagaaagt tatgctcaga ggagcgacgg tcttagccgt agtgatgttg gctgtgatag
     3301 tgtacccgag tgtgggaaat tcctttgtct acaaagtccc ttttaccatt acagcggggg
     3361 agatccaagc caacggtggt aatccatttc ccgccgttac aattgatcag gtggacttgg
     3421 gtggattgca agggcttagt gccggaccct gtcaggccaa cgcactcatc agtggcatca
     3481 aaattactag aagaggcaat gctaagcccc ttctttccaa tgtccaacct cctggctacg
     3541 cggtggccgt gacaatctct agagatttgg gattgggtga agttgccctt ccagcaggtg
     3601 agtacgacgc gtggttcaaa agcgacaaag gaccgtgcga caccccgaaa gcacgaatta
     3661 ccaaaaaagg gagtggcgct ttcagtattt ctacggtcgc cgcggtaaca acgattgaca
     3721 cggcaacggt tgagcctgtt cagttgccga cgctaaatca gtggggcttg attctccttg
     3781 tcgctgttct gggtggaatt gggactttcg tcatagttcg tcgtcgttgg acagcagtat
     3841 aaggactgtg aggaggttga ccaaggaata ggcaaagacg gggctcgcaa gaaagggaga
     3901 gagatctatg cgtcgaggag agaagctgta cgggtgcctt tgtgcggtta tgttagttag
     3961 tacagcaggc actgttagcc tcgctaagtc gccacgtgca ctaccagaca aagttgatgg
     4021 tgccgccctc tcttcttttc tgcgagcccc tacttctgaa atactccaac agtcttctgt
     4081 tgcggctgtc agcgacacct gtggtgacac ctgtgatgtc ggatatgatg atggcgtctt
     4141 gacaaccaaa gccactgcag catttggatc tccgggagat cgtctggcag tgggcattgt
     4201 acgacccgca aaattctcaa aactgatagc agcgaaatac ttcctgatat gggcaaatct
     4261 cgtgcctccc aagccaaacg gtccttttcg cgtacgagtg tttagagata gcacgggcat
     4321 tcccagtagg gctgatttaa ttccaggcac tctcacgtat ctcgctccga gtcaaggttg
     4381 gaattccgtg ccccttgctt cttacaacat catcccccct gatgacacaa cgtatgtgac
     4441 actcgagtac ctcacgggtc ccccggttgc caatcagact cccgatgtca tggtggatgc
     4501 ggccgtaaat caagatggcg gcgggttaat gtattctgta ggggtcggta attggcagtc
     4561 agtacagact acctacggac caagcgctgg cgatttgttc atacgcggtg tttttgaatt
     4621 gccccgacgt ggtgattacg gtgacgctcc ggattcatcg aacggtaaca tcgccgcggc
     4681 gggctccggc ttcccaacgc agtacttcac caagaacgct accgttatcg gcaggcgggg
     4741 tcctttccat cgggatacaa cccaattctg gcttggcgat tccggtaatg ccccgacccg
     4801 tgaacgcaac cctcagaaca acacggggga cggtgatcgc gatagttcaa agattaacgg
     4861 gcaattctac accgctgtcg tgggcagcga tacgctcggc tggttcatcg tcccggtttc
     4921 tgtggccggg tcagcgagcg ctgccgtcag ggtccttaac atcctttacg accaaaacaa
     4981 gaatggtaag tggcagaata ccagtgcggc ctttccagag tggctcgtcc gcaacgcaca
     5041 ggttttcacc aacccgggaa tgacggaaaa ctacgtcctc ggccccttcc ggctcaagaa
     5101 tgtgccgccc ggacctatct ggagccgctt taccttcacc agcgattcta ttcctcaggc
     5161 actgtttggg accggctggg acggcagcgg cccggatacc ggttttgcgg ccggtgagac
     5221 ggaagacttt aatttactgg gaagcgtttc ggctgaatcg gcggcgctgg tccaggtggc
     5281 cgcaccagtt accttcgacg cgtttgaagc ggacaccggc cggagcacac gagaggtccg
     5341 gttcatcaat tacggaggga aagccctgac cgcctcggaa attcgtttct ttgcgctcgg
     5401 agaccccgat actaacttca tacttactct ggtggattct gccggtcaaa attctacgaa
     5461 ctttaccatt ccggcaaatt cgaccgttac tttccgttac cgcgcgcagt tcaatcggag
     5521 ttcggacgat accgtccacc gcgcggaccg aacccgcgcc tttgcctggc aactgcgcgg
     5581 taattccatc actaccccgc ttttgtcgca ggcttttacc acctttgtcg aacatgtccg
     5641 acctcagttt gtccagccgg ttcagaattc ctttacccgg tctgcctgtg atttctcttt
     5701 cgatttcctg gtgacggcaa tggatattga cgaggaaata ttgttcccgc tcaatatccg
     5761 ttactgtggt acttgcttcc gggagacggt cagtctgcgg gctccggtta acttaccaac
     5821 atttttcccg gataccgtgg ctgctcttgg cacaaacaac ccggggagat ttcgctggac
     5881 gccgggcgtc gcggacacag gtgtctggct ggcgaaattc gtcgcacggg atagcgtgga
     5941 caagatggaa gataccaccg atattttcat ctccgtcagt gcgacctcag ccaccggagt
     6001 gattgcagtg aggcatatcg tggtcaccgt tccggagagc accacctgtt tttcggtagc
     6061 ggtgcgggtt ttcaatgctg attttctccg aacgatcaaa atcccccttg tctacgattc
     6121 gttaatttgt ctgaaagcca tcacatacga cagtacccgt ttagcaaatt caaacatttt
     6181 gaaccaacga ttcctcgaca gcacaagcta caggaaccac aatggtctgg atacgttggt
     6241 gttaaccttt ttcagaaccg tcgggtttag tgatctggag cccggttgtg gaacgattgc
     6301 caatatttgg ttcagcacta aagtaactga cacaactgtc aacccactgg acaccgtttt
     6361 cattaactcg tcagagcgtc ccttcttcga aactgccgga ggccaggagg ttctcaatct
     6421 ccaatttcaa aacgaaccgt tgcatccgca agtactccta ggagatttgg attgcgacac
     6481 ggtaagagac atcaatgaca ttgtgggact catcaattgt gttgtgttct tccccgatcc
     6541 caataaatgc acccta
//
LOCUS       DBNGZA010000508         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_421066, whole genome shotgun sequence.
ACCESSION   DBNGZA010000508 DBNGZA010000000
VERSION     DBNGZA010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_421066"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC16FU_10825"
     CDS             complement(<1..530)
                     /locus_tag="AC16FU_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994901.1"
                     /translation="MKRIFPFLLILASPVVVGRELATAQIQQAEKFIAEHEYGKAYTV
                     LSELVAKDPKNAQAVLWAAEAAPTGVDAQQVLEQYQATGSLNLAVVSELVRLYYAIGD
                     YGKIMETTERVEKQLKNKPLPADVLFLAGQAAGALGSAEWAQYYFDLLQQQRPGSLFA
                     GWAELGSADLLAESQRT"
     gene            complement(544..1698)
                     /locus_tag="AC16FU_10830"
     CDS             complement(544..1698)
                     /locus_tag="AC16FU_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR2994902.1"
                     /translation="MFMQLTHLFWIGILTFVVLLIIIFFWTRAQKEKTQIAPALYVEA
                     LQALVGGDEPLAFQKLKQVVAEDSSNLDAYLKIGDTLRRRNKPAQALQVHQDLLLRPD
                     LDAATRTAVLKSIAQDYLAGENYSRAEKILHDLAQADGHNPWVYRQLLSVCEAQKDWE
                     GAFAAAERLMKLGGEKNLKVLAKYKMLLGQKLVAAGDYHKARIAFKEALDYDLQAVPA
                     YLAIGDAYAEERRTDDAVAFWRKVLEVEPKAGYLVFGKLEKAYFELGQFNEVTEIYQQ
                     VLKRDPKNVPARYGLGQIFEKKGMIQPAIEYFTSLLDLDPSFMPARLNLIRIFRSQKR
                     YDLALDVVESFLEHLVQPSRSYACEACGAEVSEPTWVCPKCRAWNSFPLW"
BASE COUNT          348 a          531 c          488 g          380 t
ORIGIN      
        1 gtccgctgac tttcggccag aaggtcggcc gaaccgagct ccgcccagcc ggcaaagagc
       61 gacccgggtc gctgctgctg caaaaggtcg aagtagtact gcgcccattc ggctgagccc
      121 agcgctccgg cggcctggcc agccaaaaag agcacgtcgg ccggcagcgg cttgttcttc
      181 agctgcttct ccacccgctc ggtcgtctcc atgattttcc cataatcgcc gatcgcgtag
      241 tacaaccgca ccagttccga caccacggca agatttagac tgccggtggc ctgatattgc
      301 tccagcactt gctgcgcatc cacgccggtg ggggcggcct ccgccgccca gaggacggct
      361 tgcgcgtttt tggggtcttt cgccaccagt tcggaaagaa ctgtataggc ctttccatac
      421 tcgtgctcgg caatgaactt ctcggcttgc tgaatttggg ctgtcgctaa ctcacgacca
      481 acaaccacag ggctcgccaa tatcaggagg aatggaaaaa ttcttttcat tgggcaattc
      541 aggttaccag agcggaaacg aattccaagc ccggcacttg gggcagaccc aggtcggctc
      601 ggagacttct gccccgcagg cttcgcaggc gtaactccgg gagggctgga cgaggtgttc
      661 cagaaacgac tccaccacgt ccaaggccag atcgtagcgc ttctggctgc ggaaaatccg
      721 gatcaagttc aagcgggcgg gcataaagga ggggtcaagg tcgagcaggg aggtgaaata
      781 ctcaatcgcc ggctgaatca tccctttctt ctcaaagatc tgacccaagc cgtagcgggc
      841 cggcacgttc ttcgggtcgc gcttgagcac ctgctggtaa atttcggtca cctcgttgaa
      901 ctgtcccagt tcaaagtagg ccttttccag tttgccgaag acaaggtagc ccgctttcgg
      961 ctcaacttcc agcaccttcc gccagaaggc caccgcgtca tcggtgcgcc gttcctctgc
     1021 gtaggcgtca ccgatagcca gataagccgg caccgcctgg aggtcgtaat ccagcgcttc
     1081 cttgaaggca atgcgcgcct tgtgatagtc gcccgcggcg acgagttttt ggcccaatag
     1141 cattttgtat ttcgccagga ctttgaggtt tttctccccc cccaatttca taagcctttc
     1201 cgccgcggca aaggcccctt cccaatcctt ttgcgcttcg cagacggaga gcagttgccg
     1261 gtagacccag ggattgtggc cgtcggcttg agccagatcg tgcaatattt tttctgcccg
     1321 gctgtaattt tctccggcga gataatcctg cgcaatgctt ttgagcaccg ccgtccgcgt
     1381 ggcggcatcc aggtcaggcc gcaggagcaa atcctggtgc acctgcagcg cctgcgccgg
     1441 tttgttccgc cggcggagcg tgtccccgat tttcaggtag gcgtcgaggt tggaagaatc
     1501 ctccgccacc acctgcttta acttttggaa cgcgagcggc tcatcccccc cgactaaggc
     1561 ctggagcgct tccacgtaga gcgcgggcgc aatttgggtt ttctccttct gggcccgggt
     1621 ccagaagaaa atgatgatca ggagcaccac gaaggtgaga atcccgatcc agaacaggtg
     1681 tgtcagttgc atgaacatgg tcaatcaccc actgaaatgc gaagcgatgc tacacttctt
     1741 ctgtttc
//
LOCUS       DBNGZA010000509         5228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2646994, whole genome shotgun sequence.
ACCESSION   DBNGZA010000509 DBNGZA010000000
VERSION     DBNGZA010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2646994"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="AC16FU_10835"
     CDS             complement(<1..610)
                     /locus_tag="AC16FU_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR2994903.1"
                     /translation="MATDQLLDIGQQYETKYGFHDPEKYVFKAKPGLSQQVVEGISSM
                     KGEPEWMRKFRLRALDIFLKKPMPTWGNTELLTSIQFDTIHYYLKPMEEQGRSWDEVP
                     ADIKRTFDRLGIPEAERKFLAGVSAQYESEVVYHSIREDLSKQGVVFLDMDSGLREYP
                     DLVKRYFGTIIPAGDNKFSALNSAVWSGGSFVYVPPGVHVRVP"
     gene            complement(681..1454)
                     /gene="sufC"
                     /locus_tag="AC16FU_10840"
     CDS             complement(681..1454)
                     /gene="sufC"
                     /locus_tag="AC16FU_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR2994904.1"
                     /translation="MSQNRPVLVIQDLHVGIEGKEILKGVDLRINKGEVHALMGPNGS
                     GKSTLSNALMGHPKYQVTRGQVLFKGENIIEMKADERARKGIFLAFQYPTPIPGVTVT
                     NFMRSSLKAVKGKEIPPREFLKLLREKMKTLEIDEEFAHRYVNDGFSGGEKKRHEILQ
                     MAMLEPEMAILDETDSGLDIDALRIVAKGVNQLIGPNLGVLIITHYQRILNYITPDFV
                     HVMADGQIVRSGDRKLAHDLEEKGYDWLLPETQKEAVPA"
     gene            complement(1540..2016)
                     /locus_tag="AC16FU_10845"
     CDS             complement(1540..2016)
                     /locus_tag="AC16FU_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR2994905.1"
                     /translation="MRITKQEEYGLRCILQLAQNNGDAPLTIPEIASREGLSIDYATK
                     LLILLRRSGLVQSVRGVKGGYRLAKRSENTSVREVLSALGWSFLDEDLCSHYPGKMQE
                     CVHLGGCGIRPIWSTVARQIYALLDKTTLAQLLQEEQMVSHQLEARVQAKTGIERG"
     gene            2371..3135
                     /locus_tag="AC16FU_10850"
     CDS             2371..3135
                     /locus_tag="AC16FU_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HIR2994906.1"
                     /translation="MYLLYVDESGDPTDKSQEYFVMGAVSVFERQTYFLSEEFDKVQE
                     KWFPGTVEPIEFHTFDIRSHGTEPWHSMHSQKRKGLLSDLCAVLENAYPSGVCLFGVA
                     LHKPSFPKDDPVERVFHELCGHFDAFLSRINTEKERDVQRGLMILDSSKYTGRLDKLL
                     LQYRREGTRFGRVRNFADAPCFADSKTTRLLQAADLIAHAIFRRYERSDAWLFDRIVS
                     RFDKSEGIIHGLTHMIAKYRECPCPACISRISRPRQ"
     gene            complement(3320..3904)
                     /locus_tag="AC16FU_10855"
     CDS             complement(3320..3904)
                     /locus_tag="AC16FU_10855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR2994907.1"
                     /translation="MKNPPPITYHPFTSSLWPDLERLFGPNGACAGCWCMWWRIPHGG
                     KLWREMQGQKAKRSLKQLVRKRKAFGVLAYAGDEPVGWCTFGPRIDFPRLQRVRAYTR
                     DDIEKVWSIPCFFIHRNWRSQGLARGLLTEAIRLARKKGAKIVEGYPVPLTRAGKKLP
                     AAWSYTGPLGIFEEQGFRIVQRVSRSAPLVRKKL"
     gene            complement(4079..>5228)
                     /locus_tag="AC16FU_10860"
     CDS             complement(4079..>5228)
                     /locus_tag="AC16FU_10860"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HIR2994908.1"
                     /translation="LREHNPELQVIVITAFGTVETAVEAMKAGAFHYITKPIDLEVLS
                     ATLTQALEKQTLLSENRVLREELEKIAPDETIVGNSAGMRQVLATVARVAPTDSTVLI
                     RGESGTGKELIARALHRMSPRVSKRFVAINCAALPESLLESELFGHEKGAFTGALKQK
                     PGRFELADGGTLFLDEIGDLSPALQAKLLRVLETHEIERLGGTAALPVDVRVICATHQ
                     NLEQRIKEKTFREDLFFRLNVIEIPIPPLRERRDDIPLLVQHFLEKFQKKTGKKISGL
                     TPEAKQLLLNYAWPGNVRELENLLERACVLARGEVLGIADFPPLTGKNVREKTDESEP
                     VSLKDLERQQIVKMLQRYDGNLGQTAQALGIHRNTLRMKMREYGITAP"
BASE COUNT         1092 a         1411 c         1422 g         1303 t
ORIGIN      
        1 ggggaactct gacgtgcacg cccggcggca cgtagacgaa cgaaccgccg ctccagacgg
       61 ccgagttcaa cgccgagaat ttattgtcgc cggccggaat gatggtgccg aagtagcgct
      121 tgaccaagtc cgggtactcg cgcaggccgg aatccatatc caggaaaacg acgccttgct
      181 tggacaggtc ctcccgtatg gagtggtaga ccacttccga ctcgtactgg gccgagacgc
      241 cggccaaaaa cttgcgctcc gcttccggaa tgcccaaacg gtcaaaggtc cttttgatgt
      301 cggccggcac ctcgtcccac gagcggccct gctcttccat cggcttgagg tagtagtgga
      361 tggtgtcgaa ctggatggag gtcagaagtt cggtattgcc ccaggtcggc attggcttct
      421 tgaggaagat gtctaaggcc cggaggcgga atttccgcat ccactccggc tcgcctttca
      481 tgctggagat cccttccacc acctgttgtg aaaggcccgg tttggctttg aagacgtact
      541 tttccgggtc gtggaaaccg tacttggttt cgtactgctg gccgatatct aagagttgat
      601 cagttgccat ctttgcttac ctcctcagga ggttcttttg acctcaccct taccctctcc
      661 tacaggagag ggaataaaag ctatgccggt accgcctctt tttgggtttc cggcaaaagc
      721 cagtcgtacc ctttttcttc caagtcgtgc gccaattttc tgtcgcccga gcgcacgatc
      781 tggccgtcgg ccatcacgtg gacgaaatcg ggcgtgatgt aattcaaaat tctctggtag
      841 tgggtgatga tcaggacgcc cagattcggc ccgattaact ggttgacgcc tttggccacg
      901 atgcggagcg catcaatatc caacccggaa tcggtttcgt ccaagattgc catttccggt
      961 tcgagcattg ccatctgcag aatctcgtgg cgtttctttt cgccgccgga aaagccgtcg
     1021 ttcacatagc ggtgggcgaa ttcttcgtcg atttcgagcg tcttcatctt ctcgcgcaat
     1081 agtttcaaaa actcccgcgg ggggatttct ttgcctttga ccgccttcaa cgaactccgc
     1141 atgaagttgg tcaccgtcac acccggaatc ggagtcgggt actggaaggc gaggaagatc
     1201 cctttgcgcg cccgctcgtc ggctttcatt tcaatgatat tctcaccttt gaagagcacc
     1261 tggccccggg tgacctgata tttcggatga cccatcaggg cgttggagag ggtgcttttg
     1321 ccggagccgt tggggcccat caaggcgtgc acctcgcctt tgttgatgcg gagatcgaca
     1381 cccttcaaaa tctctttgcc ttcaatgccg acgtgcaaat cctgtatgac aagcaccggt
     1441 ctgttttgac tcattggcca attttctcct gtttgcgaaa ttcttcacaa gccatactat
     1501 cgatacctga aagttttgtc aagtttcgtc ccaaaaaaat taccctctct ctattcccgt
     1561 ctttgcttgc acccgtgctt ccaactggtg agacaccatc tgctcttcct ggagcagttg
     1621 ggccagggtc gttttgtcca gcagggcgta gatttgtctc gccaccgtgg accagatggg
     1681 gcggatgccg cagccgccga ggtggacgca ctcctgcatt ttccccggat agtggctgca
     1741 gaggtcttca tccagaaacg accagcccag ggcgctcaag acctcccgga cgctcgtatt
     1801 ttctgaccgc ttagccagcc ggtagccgcc tttaactccc cggacgctct gcaccaagcc
     1861 gctccgccgg aggaggatca aaagtttggt ggcatagtcg atagacagcc cctcccggga
     1921 ggcgatttcc gggatggtca aaggcgcatc cccgttattc tgagctaact ggaggatgca
     1981 ccggaggccg tattcttcct gcttagttat tctcatatct ccccttggca ggggtgagcg
     2041 aagtcgaacc cttgtttggt tattcgcaag ctatctcctt atccacacta tacatacgaa
     2101 atcctgacga gtttgttagg attcctataa agaaatatcg gcagggaagt caggtttgtc
     2161 aaggggaatt ttggggctat cttgatctct cttggtgacc attcaaaccc cttctacggg
     2221 tatccttttt ctaataacaa ttttgcttga catacgtata atggttgacg atattaggat
     2281 gagagccggg agggccgcgt tctcgtggtc cgctgaagaa gttttcaccc ggttctcttt
     2341 ttttatatct tcccacagag gaggtgaaaa atgtacttgc tctatgtgga cgaatccggt
     2401 gaccctactg acaaaagtca agaatacttt gttatggggg cagtatcagt cttcgagaga
     2461 caaacctatt tccttagtga agaatttgat aaggtacaag aaaaatggtt tcccggaacc
     2521 gtggagccta ttgagtttca tacttttgac ataaggtcac acggaacaga gccttggcat
     2581 tctatgcata gccaaaagcg aaaggggctg ctttctgacc tttgtgccgt tcttgaaaat
     2641 gcttatccct cgggggtttg tctttttggg gtagctctgc ataaaccatc ttttccaaag
     2701 gacgatcccg ttgaacgagt attccacgag ttatgcggtc atttcgatgc ttttttaagt
     2761 cgaataaaca cagaaaaaga gcgggacgtt cagcgcggtc taatgattct agattcatct
     2821 aagtatactg ggcgtttaga caaattactg ttgcaatatc gtcgtgaggg cacccgtttt
     2881 ggtagagtaa gaaacttcgc cgatgctcct tgctttgcag actcaaaaac aacgaggctt
     2941 cttcaggctg ctgacttgat cgcacatgcc atctttaggc gatatgaacg ttccgatgct
     3001 tggttatttg ataggatagt ttcccgattt gataaatctg aaggaataat tcatggcctg
     3061 actcacatga tcgcgaaata tcgtgaatgt ccatgtcctg cttgtattag tagaatttcg
     3121 cggccacgtc agtaggtagc aaatttttag accgccgtta tttcttcatc tcgagtttca
     3181 gcgcagcgga gttgatgcag tagtctgcct gaggcggact ggcgggggca gtcaggatgg
     3241 gcggggctga gagccccgcc tacgcgagaa gaaattctct cctcatcgcg taggcggcac
     3301 tcttagtgcc gccatttcct caaagcttct ttctcaccaa aggtgcggag cgtgagaccc
     3361 gctggacaat tctaaatccc tgctcttcga atattcccag cgggccggtg taagaccagg
     3421 cggcgggcaa ctttttgccc gctcgggtca aaggcaccgg gtagccctcg acgatcttag
     3481 cacctttctt tctggcaagg cgaattgctt ctgtaaggag cccgcgcgcc aggccttggc
     3541 tgcgccagtt acggtgaatg aaaaagcagg ggatcgacca gactttttcg atatcgtctc
     3601 gcgtatacgc tcgaactctc tgcaagcgag ggaaatctat tcgcgggccg aaagtgcacc
     3661 agccgacagg ttcatcaccg gcgtaggcca gaacgccgaa cgctttgcgc ttgcgaacta
     3721 attgtttgag cgaacgcttg gctttttgtc cttgcatctc gcgccacagt ttccccccgt
     3781 ggggaatgcg ccaccacatg caccagcagc cggcgcaggc gccgttcggc ccaaagaggc
     3841 gctcaagatc aggccagagg gaggaggtga acgggtggta ggtgataggt ggtggatttt
     3901 tcatttagtt ctccttttga cctcacccca accctctccc cttcggctgg ctcagggtgg
     3961 tgagcaaagt cgaaccacta caggagaggg agatatcctt actccaaaga taggcagttc
     4021 cctcccctgt agggtcgaag actcgtaccg aggagggcag ggtggggtca aaaatgcttc
     4081 acggggctgt aatcccatac tccctcatct tcatccgaag cgtgttgcgg tgaatgccca
     4141 gagcctgcgc agtctgaccc aggtttccgt cgtagcgttg cagcatcttc acgatctgct
     4201 ggcgttccag gtctttcagc gacaccggct ccgactcatc agttttctcc cggacgtttt
     4261 ttccagtcaa gggtgggaag tcggcgatgc cgagaacctc tccacgcgcg aggacgcagg
     4321 cgcgttccaa aagattttcc aattcgcgca cgttgccggg ccaggcgtag ttcagaagga
     4381 gctgcttcgc ttccggggtc aggccggaaa ttttcttgcc ggttttcttc tggaatttct
     4441 ccagaaagtg ctggactaaa agggggatgt cgtcccggcg ctcccggagc ggaggaatgg
     4501 ggatttcaat cacgttgaga cggaagaaga ggtcttcgcg gaacgttttc tctttgatcc
     4561 gctgctccag gttctgatgc gtggcgcaaa tcacccgcac gtcaaccggc aaagcggcgg
     4621 tgccgcccag ccgttcaatc tcatgcgtct ccaacacgcg caaaagtttt gcctgcaagg
     4681 cgggggagag gtcgccgatt tcgtccaaga acagcgttcc gccatcggct aactcaaagc
     4741 ggcccggttt ttgtttcaac gcgccggtga aagcgccctt ctcgtggccg aagagttccg
     4801 attctaaaag cgactccggc agggccgcgc aattgatcgc cacgaagcgc tttgagacgc
     4861 ggggcgacat ccggtggagg gcgcgggcga tcagttcctt gccggtgccg ctttcgccgc
     4921 gaatcaggac ggtggagtcg gtgggcgcca cgcgggccac ggtcgccagc acctggcgca
     4981 ttcccgcgct gttaccgaca atggtttcat ccggcgcaat tttctccaac tcttcccgca
     5041 aaactcgatt ttcgctcaaa agcgtctgct tttccagcgc ctgcgtgagg gtggcggaga
     5101 ggacttcgag gtcaatcggc ttggtgatgt agtggaaggc gccggccttc atcgcctcca
     5161 cagcagtctc gacggtgccg aaagcggtga tgacgatcac ctgcagttcc ggattatgtt
     5221 cgcgcagg
//
LOCUS       DBNGZA010000510         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_314343, whole genome shotgun sequence.
ACCESSION   DBNGZA010000510 DBNGZA010000000
VERSION     DBNGZA010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_314343"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="murD"
                     /locus_tag="AC16FU_10865"
     CDS             complement(<1..1073)
                     /gene="murD"
                     /locus_tag="AC16FU_10865"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR2994909.1"
                     /translation="MTELFSAAAPAANQSNFNGQGNHFRPDRVTGKRVGIVGMARSGL
                     ACARLLANQGAAVFLSDTKSAGQLQTEISQLQKWGLEYEAGGHTRKLVTDRDFLVLSP
                     GVPPDVPICRAAEAQGIPVFSEIEVASWLCPAAIVGITGSNGKTTTTSLAGEMFRNAG
                     YHTLVAGNIGTAFSSVVEQATKASVVVLEISSFQLEKIEEFRPRTAVLLNLSPDHLDR
                     YGNYEAYKQAKLRIFENQKETDFAVLNADDLGLSDLPPKLAAKVAFFSTKGEVGFGAC
                     IRQGEICLKRNGHYDTVLPAAKVGIPGPHNLSNATAACAIGLSFGLAPEVMARALAEF
                     KGVEHRLEFVAEIEGVKFVNDSKA"
     gene            complement(1073..2158)
                     /gene="mraY"
                     /locus_tag="AC16FU_10870"
     CDS             complement(1073..2158)
                     /gene="mraY"
                     /locus_tag="AC16FU_10870"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR2994910.1"
                     /translation="MLYHLFYPLSQYISGFNLFRYITFRTAGATVTALFIAWVIGPYI
                     IRKLRARQVGEKIRAEGPQTHLKKEGTPTMGGIIILAAVVVPTLLWADLSNRFVQVAL
                     LVTVWMGVVGFVDDYLKVIKGKRKGLVARNKFSGQILLGLIVGAILYFFPPAKEFTPD
                     TDIPFFKNYYIPFGALYIPFVVLIITGTSNAVNLSDGLDGLAIGLVGICFFAFAGLAY
                     LTGRVDFSHYLTITYLEGAGELTIFCGAVLGAALGFLWFNAHPAEIFMGDTGALALGA
                     ALGTVAVLIKKEILLVIVGGVFVAEAASVILQVVSFKTRGKRIFKMAPLHHHFELLGW
                     AEPKVVVRFWIAGALLALLTLSTLKIR"
     gene            complement(2166..>2514)
                     /locus_tag="AC16FU_10875"
     CDS             complement(2166..>2514)
                     /locus_tag="AC16FU_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR2994911.1"
                     /translation="NPPKRDSGRRVAVLADMLELGEKQETLHREVGSVAAKAGVDFLI
                     AVGPLAGEYLAGATPVGMFSSQMRHFENNPAALDFLLGWLRAGDLVLVKGSRGMKADA
                     IVTGLKELLPKQS"
BASE COUNT          546 a          780 c          709 g          479 t
ORIGIN      
        1 gctttcgaat cgttgacgaa tttcactccc tcgatttccg ccacgaattc taagcggtgc
       61 tcgacccctt tgaactcggc caaggcccgg gccatgacct ccggcgcgag gccgaaggaa
      121 aggccgatgg cgcaggcggc ggtcgcattc gacaggttat gcggccccgg gatgccgacc
      181 ttcgctgctg gcagcaccgt gtcgtagtgg ccgttgcgct tgaggcagat ctcgccctgg
      241 cggatacagg cgccaaaacc gacttctcct ttggtggaaa agaatgccac tttcgccgcc
      301 aatttgggcg gcagatcgga aagccctaag tcgtcggcat tgagcaccgc aaagtcagtc
      361 tccttttggt tttcaaaaat ccgcagtttg gcctgtttgt atgcctcgta attcccgtac
      421 cggtcgaggt ggtcgggcga gagattgagc aacactgccg ttctggggcg gaactcctcg
      481 atcttttcca actggaaact ggaaatttcc agaaccacga cgctcgcctt agtcgcctgc
      541 tccaccaccg aggaaaaggc cgtgccgatg ttccccgcca cgagcgtatg gtaccccgcg
      601 ttccggaaca tttcgcccgc caggctggtg gtggtggttt tgccgttgga gccggtgatg
      661 ccgacgatcg ccgccggaca gagccaggag gccacttcaa tctcggagaa aaccggaatc
      721 ccctgggctt cggcggcccg gcaaatcggc acgtccggcg gcaccccggg tgaaagcacc
      781 agaaaatcgc ggtcggtgac cagtttccgg gtatgccccc cggcctcata ttccagaccc
      841 cacttttgga gttggctgat ttccgtctgc aactgtcctg ccgacttggt gtcgctcaaa
      901 aagactgccg caccctgatt ggccaaaagc cgggcgcagg ccaggccgga gcgggccatg
      961 cccacaattc ccacccgctt ccccgtcacc cgatcgggcc ggaagtgatt gccctgaccg
     1021 ttaaagttgc tctggttcgc cgccggagcg gctgccgaga agagttcggt catcagcgaa
     1081 tcttgagcgt gctcaaggtt aaaagggcca gaagcgctcc cgcaatccag aagcgcacca
     1141 ccaccttcgg ctcggcccag cccaagagtt caaaatggtg gtgcagaggc gccattttga
     1201 aaatccgttt gccgcgggtc ttgaacgaca ccacctggag aatgaccgag gccgcttcgg
     1261 ccacgaagac cccgcccacg attactaaga gaatctcttt tttgattaaa acggcgaccg
     1321 tccctaaggc ggcccccaag gcgagcgcgc cggtgtcacc catgaaaatt tcggccggat
     1381 gcgcattgaa ccagaggaaa cccaaagctg cgcccagtac cgcgccgcag aaaatggtca
     1441 gttcgcccgc gccttcgagg taggtgatgg tcaaatagtg gctgaaatcg acccggccgg
     1501 tcaggtaggc cagcccagca aaagcaaaaa agcaaatgcc cacgaggcca atggccaggc
     1561 cgtccaaccc gtcgctcaag ttcacggcgt tggaggtgcc ggtgatgatc aaaacgacaa
     1621 agggaatgta aagcgcgccg aagggaatgt agtagttttt aaagaagggg atgtcggtgt
     1681 ccggagtgaa ctctttggcc ggggggaaga agtagaggat ggctccgacg atcaacccta
     1741 aaaggatttg acccgaaaac ttgttgcgcg ccaccagccc cttgcgcttg cccttgatca
     1801 ccttcaggta gtcatccacg aaccctacga cccccatcca gaccgtcacc aaaagcgcca
     1861 cctgcacgaa acggttggag aggtcggccc acagaagcgt cggaacgacc accgccgcca
     1921 aaatgatgat cccgcccatg gtcggcgtcc cttccttctt gagatgggtc tgcggccctt
     1981 cggcgcgaat cttctccccc acctggcggg ctctcaattt ccggatgata tacggcccga
     2041 tcacccaggc gatgaaaaga gccgtcaccg tggctccggc ggtccggaag gtgatgtagc
     2101 ggaaaaggtt gaagccggaa atgtattgac tcaaggggta gaacaggtga tagagcacta
     2161 tcctcctatg actgtttcgg taagagttct ttcaaaccgg tgacgattgc atcggctttc
     2221 atcccgcggg aacccttgac caagaccaga tcgcccgctc tgagccagcc caaaaggaag
     2281 tcgagcgccg ccgggttatt ctcgaaatgt ctcatctgcg acgaaaacat ccccaccgga
     2341 gtcgctccgg cgagatactc gcccgccaaa ggccccacgg cgattaagaa atcgactccc
     2401 gctttggccg cgacgctgcc cacttcccgg tgcagcgttt cctgcttttc ccccaactct
     2461 aacatatccg ccagcaccgc cacccggcgg ccggagtctc gtttcggcgg attc
//
LOCUS       DBNGZA010000511         1353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2014762, whole genome shotgun sequence.
ACCESSION   DBNGZA010000511 DBNGZA010000000
VERSION     DBNGZA010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2014762"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="pyrR"
                     /locus_tag="AC16FU_10880"
     CDS             <1..539
                     /gene="pyrR"
                     /locus_tag="AC16FU_10880"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR2994912.1"
                     /translation="QVMDDKALARALVRIAHEIVEKNHGARGLVIVGIKTRGAHLAER
                     IAEIIAEIEKTKVPTGSLDITFYRDDIQTRLEHPVVQKTELLVPVEGQTVVVVDDVLF
                     TGRTIRAAMDELIDFGRPKQIQLAVLIDRGHRELPIRADFVGKNIPTAPEDNVIVQIK
                     EIDRKDQVLVERKSKGKK"
     gene            559..>1353
                     /locus_tag="AC16FU_10885"
     CDS             559..>1353
                     /locus_tag="AC16FU_10885"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR2994913.1"
                     /translation="MPLKSRHLLGLEGMTREEIDLVLNTADTFKEVLERPIKKVPPLR
                     GTTVVNLFYEPSTRTRISFELAEKRLSADTVTFTPSGTSVAKGETLRDTTRNLQAMKI
                     DMVVVRHKSPGVPYFLTQCIDASIINAGDGAHEHPTQGLLDLYTIREKYGKIEGLRVV
                     IVGDIQHSRVARSNIWGLKTMGASVAVCGPTTLLPVEISKMGVEVYANLDDTLIGADV
                     VNVLRLQLERQQSGLLPSLREYAIRFGITAERLKRLNPNFTIMHPGP"
BASE COUNT          309 a          417 c          381 g          246 t
ORIGIN      
        1 cgcaggtgat ggacgacaag gcgctggccc gtgctctggt gcgcattgcc catgaaatcg
       61 tggagaaaaa ccacggcgcg cgcggactgg tcatcgtcgg tatcaagacc cgcggcgctc
      121 atttggctga acgcatcgcc gaaattattg ccgaaatcga gaagaccaaa gtccccaccg
      181 ggtcgctcga catcaccttc taccgcgacg acatccagac acgcctcgaa catccggtgg
      241 tccagaagac ggaattgttg gtacctgtgg aggggcagac ggtcgtcgtg gtggacgacg
      301 ttctcttcac cggccgcacc atccgcgcgg cgatggacga gttgatcgac tttggccggc
      361 ccaaacagat tcaattagcg gtcttgatcg accgcggcca ccgcgaactc cccattcgcg
      421 ccgacttcgt ggggaagaac attcccacgg cgccggagga caacgtgatc gtacagataa
      481 aagaaatcga ccgcaaagat caggtgctgg tcgaacgcaa aagcaaaggc aagaaatgaa
      541 atcggatcgt cgctgcctat gccgctgaaa agccgccacc ttctgggtct ggaaggaatg
      601 acccgcgagg aaatcgacct cgttctgaac accgccgaca ccttcaaaga ggtcttggag
      661 cggccgatca aaaaagttcc gcctctccgg gggactacag tggtcaatct tttctacgag
      721 ccttccaccc gcacccgcat ctcgttcgag ttagccgaaa agcgcctctc cgccgacacc
      781 gtcaccttta ctccgtcggg cacttcagtc gccaaaggcg agaccctgcg cgatactacc
      841 cgcaatcttc aggccatgaa aatcgatatg gtggtggtcc ggcacaaatc gccgggggtg
      901 ccgtactttt tgacgcagtg tatcgacgcc tcgatcatca acgccggcga cggcgcacac
      961 gagcacccga cccagggact tctggacttg tacaccatcc gcgagaagta cgggaaaatc
     1021 gaaggcctgc gggtggtgat cgtgggcgac atccagcaca gccgcgtggc ccgctcgaac
     1081 atctggggtt tgaaaacgat gggtgcttca gtggcggtct gcggccccac cacgcttctg
     1141 ccggtggaaa tctcaaaaat gggggtcgag gtctacgcta acctcgatga caccctgatc
     1201 ggcgccgacg tggtcaacgt cctgcgcctg caattggaac gccagcagtc gggactactc
     1261 ccttccctgc gcgagtatgc catccgcttc ggcattacgg cggaacggct caaaagactg
     1321 aatccaaatt tcaccatcat gcacccgggg ccc
//
LOCUS       DBNGZA010000512         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2330507, whole genome shotgun sequence.
ACCESSION   DBNGZA010000512 DBNGZA010000000
VERSION     DBNGZA010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2330507"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="AC16FU_10890"
     CDS             <1..1884
                     /locus_tag="AC16FU_10890"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR2994914.1"
                     /translation="TGRAQIGPPVITVAYPKDSARLGASDSTFIFGQVIPRCSLFING
                     QSVPVYRNGTFVAFLPLTPGPFTFNLAASKGPDTTFKQVLVFVPEPVTTPSPETLRIA
                     GGLRPSDDLELRAGDVLTVAFRGTPGCQAFFRIDGLTDALPMAEAGAVVESSATESVF
                     QAHLADTVRPVRGFYRGAYQIQPADSIDTARIIVTLVRSPVTADAAEFAALIRDTTLI
                     RIEAVAHGTPDTLCFSFCVRDTAPGRISIRRSAVPQVVELTDSIQILRTGPGQGFLAT
                     FQPQGIKALYTGRSGQWVRLKLAEGQEAWTSQDKIRFLPPGTPPPTSTVTLIRVFEEA
                     RAVRIELPLSEKLPFRVDEELDPPQQRLTVYGLTSAVDWIRQYLESDLVRLVSWEQPQ
                     KGVFRLTCELKPGQLWGYDVSYEKNTLVWVINKQPAWLGRLKGLVFTIDPGHSADQGA
                     LGPTGLEERQANLWLAIKVADLLRKKGATVVMTRTGMEDVPLMERPKIAKKAGTHIYL
                     SIHNNALPDGVNPFFNNGTSVYYYHPHSRALAEAIHKRMVAGTGLPDFGLYYANFAVD
                     RPTQYLAVLVECAFMMIPEQEEKLRSEAFQEKVASLIVAGVEDFLLSVKKKPQTKAPE
                     SPR"
     gene            complement(1924..2745)
                     /locus_tag="AC16FU_10895"
     CDS             complement(1924..2745)
                     /locus_tag="AC16FU_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR2994915.1"
                     /translation="MPRRTAKIFWTALLSLLLLTLLFPLGWMVVTSFSAPGGSGGLAA
                     TFLQGKWTLANFRDVFSQKIFLRPLLNSLFVTALITIGNLIFCLSVGYAFARYRFRGK
                     GVLFAAVLGVLMIPQHIVIIPLFLLMQKFGWYDTYFALVIPWLVNPLGVFLLRQYIEN
                     LPRDLEDAARLDGAGILRILYTVVAPLCKPALAVLAIQIFLVVWNWFLFPFILTSSET
                     LRTLPVALALYQGYQTIDWPHLMAASTLATLPVVAVFLFFQRQIISGITAGALKQ"
BASE COUNT          626 a          807 c          821 g          529 t
ORIGIN      
        1 actggccgcg ctcagatcgg gccgcccgtt atcaccgtgg cgtaccccaa agacagcgcg
       61 cggctgggtg cgtccgattc caccttcatt ttcggacagg ttatcccgcg ctgctccctt
      121 ttcatcaacg ggcagtccgt tccggtttac cgcaacggaa cattcgtcgc gttcttgcct
      181 ctgacccccg ggccgttcac tttcaatctc gccgcgagca aagggcctga caccactttc
      241 aaacaggttc tggttttcgt gccggagccg gtgacgacac cgtcgccgga gacgttgcgc
      301 atcgccggcg gcttgcgtcc cagcgacgat ttagaacttc gcgccggcga cgttctcacg
      361 gttgctttcc gcgggacgcc cggatgtcag gctttcttcc gcatcgacgg cctcaccgac
      421 gcacttccta tggccgaggc cggagcggtg gtcgaatcat cggcgaccga aagtgttttt
      481 caagcccatc tagccgacac cgttcggccg gtgcgcggtt tctatcgcgg cgcgtatcag
      541 attcaaccag ccgattcaat cgataccgcc cgcattatcg tgacgctcgt gcgcagtccc
      601 gtgacggctg atgccgcgga gtttgcggct ttaatccggg atactacgtt gatccgtatt
      661 gaagcggttg cgcatggcac tccggacacg ctctgcttct ctttttgtgt tcgggacacg
      721 gctccgggaa gaatcagcat ccgcaggagc gcagtgccgc aggtggttga attgactgac
      781 agcatccaga ttctgcggac cggcccgggt caggggtttc tggccacctt tcagccgcag
      841 ggtatcaaag cgctctatac cggcagaagc gggcagtggg tcaggttgaa attggccgaa
      901 ggccaggagg cttggacgag tcaggataaa atccgcttct tgccgcccgg cacgccgccc
      961 ccgacttcga cggtcaccct catccgggtc tttgaagagg cgcgcgcggt gcgcattgaa
     1021 ctgcctctgt ccgaaaaact gccctttcgc gtcgatgaag aactggatcc gccgcaacaa
     1081 cgcttgactg tctacggact cacctcggcg gttgactgga tcagacagta cctggagagc
     1141 gatctggtcc gcctggtctc ctgggagcag ccgcagaaag gggttttccg cctcacctgc
     1201 gaattgaaac ccggtcagtt gtggggctac gacgtttcct acgagaagaa caccctggtc
     1261 tgggtgatca ataaacagcc ggcctggctc ggccgcttga aaggtctggt ctttaccatc
     1321 gatcccggcc actccgccga tcagggcgcc ctgggcccca ccggtctgga agaacggcaa
     1381 gccaatctct ggctcgccat caaagttgcc gaccttttgc gcaaaaaagg agccacggtg
     1441 gtaatgaccc gcacggggat ggaagacgtg cctttgatgg agcggcccaa aatcgccaag
     1501 aaagcgggaa cgcacattta cctgagcatc cacaacaatg cactgcccga cggggtgaat
     1561 cccttcttca acaacggcac cagcgtctac tactaccacc cgcacagccg ggcgctggcc
     1621 gaagcgattc acaagcggat ggtggcgggc accgggttgc ccgacttcgg gctctactac
     1681 gccaatttcg ccgtggatcg tccgacgcag tacttagcgg ttttggtgga atgcgccttc
     1741 atgatgattc cggaacagga ggaaaaattg cggtccgaag ctttccagga aaaagttgca
     1801 tcgctgatcg tagcgggagt agaagatttc ctgctttcgg taaagaagaa accgcagacg
     1861 aaggcgccgg agtctcctcg gtgaaatatt aattgaccag taggggttca acgcgttgaa
     1921 cccctactgc ttcaacgccc ccgccgtaat ccccgaaata atctgccgtt ggaagaaaag
     1981 gaaaaccgcc accacaggca gagtcgccag tgtagaggcg gccatcaggt gcggccagtc
     2041 gatggtctgg tacccctggt agagggccag ggccaccggc agggtgcgga gcgtttcgct
     2101 ggaggtgaga atgaaaggga agaggaacca gttccagacc acgaggaaaa tttgaatcgc
     2161 caagaccgcg agggccggtt tgcacaaagg cgccactacg gtgtagagaa tccgcaaaat
     2221 gccggcgccg tcgagccggg cggcatcttc gagatcgcgc gggaggtttt cgatatactg
     2281 tcgcaagagg aaaacgccca gcggattcac cagccagggt atcaccagtg cgaaataggt
     2341 gtcgtaccag ccgaacttct gcatcaaaag aaagagggga atgatgacga tgtgctgggg
     2401 aatcatcaag accccaagca cggcggcgaa gagcacccct ttgccccgga accggtagcg
     2461 agcaaaagca tacccgactg agaggcagaa aatcaggttg ccgatggtaa tcaaggcggt
     2521 gacgaaaagg gaattcaaaa gcgggcgtag aaaaattttt tgtgaaaaga cgtcacggaa
     2581 attggccaga gtccatttgc cttgcaagaa ggtcgctgcc aaaccgccgg agccccctgg
     2641 cgcagaaaaa gaagtcacca ccatccaccc caaagggaag agcagggtga gcaaaaggag
     2701 gctcaagagc gcagtccaga agattttcgc ggttcggcgg ggcatctcag tcggcaatcc
     2761 ctttccccag ccgaaacaag cgc
//
LOCUS       DBNGZA010000513         5569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_102648, whole genome shotgun sequence.
ACCESSION   DBNGZA010000513 DBNGZA010000000
VERSION     DBNGZA010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_102648"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /locus_tag="AC16FU_10900"
     CDS             complement(<1..1920)
                     /locus_tag="AC16FU_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR2994916.1"
                     /translation="MSDADEQKKHFWSTLEEWEGDPGLEQIRQEEFFSKPQAFFEAAQ
                     SQPVTLLNRRTMLKMTGAGMLFAAAACARRPVQKIVPYLNAPEEIIPGNAVWYASTCG
                     CPAGCGVLVKTREGRPIKLEGNPRHPLNRGRLSARCQAAVWDLYDPDRLSGPTQVFRS
                     KLPAAPLDWKTADARIAEALKSANGRVVLLTGTLHGPARERLIADFLASFPGARHITY
                     DALSEEQIADAQETCYGKPVVPRYRFNLAETVVFLGADPLASGHSVTEFAAGFGDRRK
                     VQNGSMSRVIAFEPALSLSGSNADSHFLVKPEDLLPVALGLAHQLVIAEGKSAYARDP
                     MVRSALAPFAPEKVERETGLPAGTLRSVAADLWNARGKSLIYTGGLATQTGNGTALHI
                     AANFLNSLLENEGTTVDGTQSPSHQTRGSQAAMLELIADLQAGKVDVLLIYGTNPAYS
                     LPPSAGLAQALYRVKLAVSLNDRIDETGALCDYVLPSLHFLESWGDAEPQKGLFSLTQ
                     PAIGPLHDARGFEDSLLALAKQASVGNLGKWSGTWHDYLKETWQKEIHAKNNIAAGFE
                     DFWTSALREGVFDTVSREEARRAPRPFNARALSSVQVSKTSASDFRLVLYAPAMQYDG
                     RTNNNTWLLEMPDPVS"
     gene            complement(1934..2440)
                     /locus_tag="AC16FU_10905"
     CDS             complement(1934..2440)
                     /locus_tag="AC16FU_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR2994917.1"
                     /translation="MRLKWKILLLTLAVLLYGWWELKSVTSPLGYAPEQPLPFSHKLH
                     AGENKIPCLYCHANAERSRHATVPSMNICMGCHQVVATSKPAIVKLTEMYKKGESPEW
                     IRVHSVPDFGYFSHRWHVAKGIACQTCHGPVEQMERVVQSRLLKMGDCVTCHRQNNAP
                     TDSNTCHN"
     gene            complement(3275..4147)
                     /locus_tag="AC16FU_10910"
     CDS             complement(3275..4147)
                     /locus_tag="AC16FU_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR2994918.1"
                     /translation="MTTYPECLLRPGNTNCGGCGMSSALTMLGRVLADQPSMMVIPAC
                     CGIVAAGAYPTTSYGVPVIAATFASAPAFASGVVAAARMNGDPMNVICWAGDGGTYDI
                     GLATLSAAAERNEDVIYFCYDNEIYGNTGGQRSSATPLYARTATTPAGKTEPKKELVE
                     IMVAHRIPYAATLSIAHKEDFENKIRTALGITGFRFLLIHSPCPIGWKSEPAESIQLV
                     RLAVQSGLFPLYEVFNGLEYRLTLEPDWSDPSPYFEKQRRFDGYRIDVAQVVQQARKQ
                     YERLRSLAGLTAGK"
     gene            complement(4144..5316)
                     /gene="porA"
                     /locus_tag="AC16FU_10915"
     CDS             complement(4144..5316)
                     /gene="porA"
                     /locus_tag="AC16FU_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HIR2994919.1"
                     /translation="MSIEVVTGNHAAGHALSAAGEANRNARGVVCGIYPITPQTEIVE
                     HVANFHFTKGHVVAVESEHSAMGVCIGASLNGARSFTASSSNGLAYMAENILVAGFYR
                     LPILMVAVNRTLGPPWNIWADQGDTLMFRDAAWIQLYCEDNQEVVDSILLGFRLAEDK
                     RVLLPVMVNMDAFIISHTSMETDLPSQELVDSFLPHFELPHRIDVNRPVTIGGLNWPL
                     ETASSRVEMDQAMRLVPGIFEEHRRAFERQFGRPVHGALEAYSTDDADLILVASGSIA
                     TTARKVVQQRRATGEKIGLVKIKMFRPFPDSELRQITQRTSKIGVLDRNYSAGTGGIF
                     WQEIRASLQGKPGTLIQNYLTGLGGMDVTPQIISEVIDDLRGRSEMGLPVWKGVAV"
     gene            complement(5322..>5569)
                     /locus_tag="AC16FU_10920"
     CDS             complement(5322..>5569)
                     /locus_tag="AC16FU_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR2994920.1"
                     /translation="DERRHTFKEVHRGLDSYEEARRCLSCGVCNACDLCLSACPDGVV
                     LRVNGELVFDYDYCKGCGVCVSECPRAVIYMKTVGQE"
BASE COUNT         1180 a         1614 c         1562 g         1213 t
ORIGIN      
        1 cgagaccgga tcgggcattt ccagaagcca ggtgttgttg ttggtccggc catcgtactg
       61 cattgccggc gcgtagagga cgagccggaa gtcgctggcc gacgttttcg atacctggac
      121 gctggacaac gcgcgggcat taaacgggcg cggagcgcgt ctcgcttctt cccggctcac
      181 cgtgtcaaaa accccttcac gcaaagccga agtccagaag tcctcaaatc cggcggcgat
      241 attgttcttg gcatgaatct ctttctgcca ggtctctttc aggtagtcat gccacgtccc
      301 gctccacttt cccaggttcc ccacactcgc ctgtttagcc agggccaaga gcgagtcttc
      361 aaacccgcgc gcgtcgtgca gggggccgat ggcgggctgg gtcagactga ataaaccttt
      421 ctgcggctcg gcgtcgcccc aggattcaag gaagtggagc gagggtagaa cgtagtcaca
      481 taaagcaccg gtttcgtcaa tccggtcatt caaggaaacg gccagcttca cccgatagag
      541 ggcctgggcc aaaccagccg aggggggcaa agaataggcg ggattcgttc cgtagatcaa
      601 aagcacgtca accttgcccg cctgcagatc ggcaatcagt tccagcatgg cggcctgcga
      661 accgcgggtc tggtggctgg gactctgcgt gccgtctacg gttgtgcctt cgttttcgag
      721 cagtgagttg aggaaattgg cggcaatgtg aagcgcggta ccgttcccgg tttgcgtcgc
      781 caggccgccg gtgtagatca aacttttccc gcgcgcattc cagaggtctg ccgctacgga
      841 gcggagtgtc cccgcaggca ggccggtttc cctttcgact ttctccggag cgaaaggagc
      901 gagagcgctc cgcaccatag ggtcacgggc gtaggccgac ttgccctcag cgatgactaa
      961 ctgatgcgcc agccccagcg cgacgggtaa taaatcctcc ggtttaacca aaaagtgact
     1021 gtcggcgttg gagccggaga gcgacagcgc cggttcgaaa gcaatcaccc gcgacattga
     1081 gccgttctgc actttccggc ggtcgccaaa gccggcggca aattcggtca ccgaatggcc
     1141 gctcgccagg ggatcggcgc cgaggaaaac caccgtttcc gccagattga agcggtagcg
     1201 gggcacgacc ggtttgccat agcaggtttc ctgagcgtcc gcgatttgtt cttcggaaag
     1261 cgcgtcgtag gtaatgtgac gggcccccgg gaaagatgcg aggaagtcgg cgatcagccg
     1321 ctcccgggcc gggccgtgga gggtgccggt caagagcacc acccggccat tggctgattt
     1381 cagcgcctca gcgattctgg catcggcagt tttccaatca agcggcgcgg cggggagttt
     1441 cgagcgaaaa acctgcgtcg gtccgctgag tcggtcgggg tcgtacaggt cccacactgc
     1501 cgcctgacag cgggccgaga ggcgtccccg gttgagcggg tggcgggggt tgccttcgag
     1561 tttgatcggg cgtccctcgc gggttttcac caaaacaccg cagcccgccg gacagccgca
     1621 ggtggaggcg taccagaccg cgttccccgg gattatttct tccggggcat tcaggtaggg
     1681 tactattttt tgcaccgggc gccgggcgca ggccgcagcc gcgaacagca ttcccgcgcc
     1741 ggtcatcttg agcatcgtcc gccggttcaa aagcgtcacc ggctgggatt gggccgcctc
     1801 gaagaaggcc tgcggcttgg agaaaaactc ctcctgccgg atctgctcca ggccgggatc
     1861 gccttcccac tcctccagcg tgctccagaa atgctttttt tgttcgtcgg cgtcgctcat
     1921 caaatgctct cgctcaatta tggcaggtat tgctatccgt gggcgcgttg ttctggcggt
     1981 ggcaggtgac gcagtcgccc atcttcaaga gccgggactg aaccacccgc tccatctgtt
     2041 ccaccggccc gtggcaggtc tggcaggcaa tccccttggc gacgtgccac cggtgcgaaa
     2101 agtagccgaa gtccggcacg ctgtgaacgc ggatccattc cggcgactca cccttcttgt
     2161 acatctcggt caacttcaca atagcgggtt tggaggtcgc caccacctga tggcagccca
     2221 tgcagatatt catcgagggc acggtggcgt ggcgcgagcg ctccgcattg gcatggcagt
     2281 agaggcaggg gatcttgttc tctccggcgt ggagtttgtg gctgaaaggt agcggctgct
     2341 ccggagcgta gcccaaaggc gaagtgaccg atttcaactc ccaccagccg tagagaagta
     2401 cggcaagtgt taacaggagt attttccact tcaatcgcat aactacaaaa atttctccac
     2461 cggcgcagtg ccgacaaaac gagcgccaat acccttctca gcctgctact ttaatgatgt
     2521 tgtgccgccg gcccggccaa acccgctcac ttgtgattat tttcacaaac gtgggtacgc
     2581 tataatatca attctttttt gtcaacagct ttctctcgtc aaaataggct gcccggtgat
     2641 tctcttcgct cagtcgggag aatctaaagc ggccgcatcc ggaaagcaac cccgtagtga
     2701 gcccgcctca ggcgggcgaa cgacgagcgt ggcgccttgc cgggccaaag gcggggcggc
     2761 gcgagtgagg gtgtcatttt agcaccgtag tacaaaccaa taatggcgag aggactcacg
     2821 ggccgggtgg aagtggaaag cgaagtgggt aaagggcgaa gtttacgccg gtgttgccgg
     2881 ttcatgagtc gggattttat tttggataag tgatctggca atatggcatt tcttttctta
     2941 gtgtactgct cctcctaaat ttagacaggt gtagtgctta tttttgacat ccaatccgta
     3001 tagaatatga tggggaattt cattcccatc gcgccgtgcg gtgcgattcg gttgtagtct
     3061 tcgacccagt ccggcagctt ccgagccacc gcttcccgaa tcccccaaac tccgccccaa
     3121 agcctgcttc cagcggtaca actggttggc gtgcaaaccg tggcgccggc ggagctcttg
     3181 aaccggcagc gtcgctgttt ccatcaaacc tccttacccc attatacctg tccaaatctc
     3241 aagtggggca cggcaatcct atttgcaaaa gaagtcattt gcctgctgtc aaaccggcca
     3301 gactgcgcag tcgctcgtat tgtttccgtg cttgttgcac gacttgtgca acatctatcc
     3361 ggtaaccatc aaaacggcgt tgcttttcaa agtagggact ggggtccgac cagtccggtt
     3421 ccaatgtcag gcgatattca agcccgttga aaacttcata cagtggaaac agacctgatt
     3481 gtacggccag acgtaccagt tgtatcgatt cagccggctc cgatttccaa ccgataggac
     3541 agggggagtg tatcagcaga aaacgaaacc cggtgatgcc cagagctgta cgtattttgt
     3601 tttcgaagtc ctccttgtgc gctattgaaa gggtagcggc atagggaatg cgatgcgcga
     3661 ccataatctc aacaagttct tttttcggtt ccgttttacc tgctggcgtc gtagcggttc
     3721 tggcataaag cggcgtagcc gaacttctct gtccaccggt attcccatag atttcgttat
     3781 cgtagcagaa ataaataaca tcttcattcc gctccgcggc cgccgacaga gtggccaggc
     3841 ctatgtcgta agtgccgccg tcaccggccc agcaaatgac attcatcggg tctccattca
     3901 tgcgggcggc cgcaactaca ccgctggcaa aggccggcgc tgacgcaaat gtagcggcga
     3961 taacaggcac tccatagctg gtggtaggat acgcaccggc agcaacgatg ccgcagcagg
     4021 cgggaataac catcatactg ggctggtcgg ccagcacccg tcccagcatc gtcaaagcgc
     4081 tgctcatccc gcagccgccg caattggtgt ttcccggacg aagcaggcac tccggatacg
     4141 ttgtcatacc gccactcctt tccagaccgg gagccccatt tcggatcttc cgcgaaggtc
     4201 gtcaataacc tcagaaatta tttgcggggt aacgtccatt cctcccagac ccgttaagta
     4261 attttgtatc agagtgcccg gttttccctg aagcgaggcc cggatttcct gccagaatat
     4321 tccgccggtt ccggctgaat aattacggtc aaggacgcct atctttgacg ttcgttgggt
     4381 gatttgcctc aattccgaat cgggaaaggg acgaaacatc ttaatcttaa cgaggccgat
     4441 cttctcaccg gtggcacgac gttgctggac gactttccgg gcggtggtag cgatgctccc
     4501 cgatgcgaca agtatcaggt cggcatcgtc ggtggagtac gcttccaagg cgccgtgaac
     4561 aggtcggccg aattgccgtt cgaatgcgcg gcgatgctct tcaaaaatgc cggggaccag
     4621 gcgcatggcc tggtccattt ccacgcgcga tgacgctgtc tcaagtggcc aattcaaacc
     4681 tccaatcgtt accggacgat tgacatcaat tcgatgaggc agttcgaaat gcgggaggaa
     4741 cgaatcaacc aattcctgac tgggcaagtc agtttccatc gatgtatgcg aaataataaa
     4801 ggcgtccata ttgaccatca ccggcagcaa taccctttta tcttcagcca gccgaaaacc
     4861 taagagaatg gaatcgacca cttcctgatt gtcttcgcaa tagagctgaa tccaggcggc
     4921 atctcggaac ataagcgtat ccccctggtc ggcccaaatg ttccacggcg gcccgagcgt
     4981 ccgattgacc gccaccatca gtatgggtag tcgatagaaa ccagccacca ggatattctc
     5041 cgccatatat gccaggccgt tggaagaact tgcggtaaac gagcgcgctc cgttcagcga
     5101 tgcgccaatg cagacgccca tggcgctatg ttcactctcg accgcgacaa catgcccctt
     5161 ggtaaagtgg aaattcgcca cgtgttcaac gatttcagtt tgaggtgtaa ttgggtagat
     5221 tccacagacg actccccgtg cgttgcggtt tgcttcgccg gcggcgctta acgcatggcc
     5281 ggccgcgtga ttcccagtga ctacttctat cgacatccgg gttactcctg tccaaccgtt
     5341 ttcatgtaaa tgactgcgcg cggacattcg gacacgcaca cgccgcatcc tttgcaatag
     5401 tcataatcaa acaccagttc tccattcact cttaacacca caccatccgg acaagcacta
     5461 agacagaggt cgcaggcatt gcaaactcca cagctcagac atcgacgcgc ttcttcgtag
     5521 ctgtccaggc cccggtgcac ttccttaaag gtgtgccgac gttcatcca
//
LOCUS       DBNGZA010000514         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_950372, whole genome shotgun sequence.
ACCESSION   DBNGZA010000514 DBNGZA010000000
VERSION     DBNGZA010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_950372"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2005)
                     /gene="metG"
                     /locus_tag="AC16FU_10925"
     CDS             complement(71..2005)
                     /gene="metG"
                     /locus_tag="AC16FU_10925"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR2994921.1"
                     /translation="MPNRIFLTTPIYYVNAEPHIGHAYTTIVADLLTRFHQARGDEIF
                     FLTGTDEHGANVAAAAKKAGLSPQEHCDRIAGLYRRTWQALSIKEDYFVRTTDKRHQV
                     AVEKFLLKLHESRTPKGEPIIYEGKYQGLYCLGCEKFLTEKELVNGLCPLHQTKPQLV
                     SEKNYFFRLSAYLDEVQRRIETDQICIRPEERKRETLGLFKQGLEDFSLSREKVTWGI
                     PLPFDPKQTAYVWVDALLNYLSAIGYGDDAKQFDTWWNQAKVVHLMAKEILKFHAIYW
                     PALLLAAGIKPPEFLFIHGFFSVNGQKMSKSLGNVIDPHHLVSRYGPDGTRFLLLTQF
                     PFGVDGDIQAERFTEKYNADLANDFGNLASRVFKLVADHFNGEVPQPGKPTHEDKDFQ
                     KIALNLAEKTLTAYVGFAGENGRYVYNDALAYTSGLVKEANRYMETQAPWALAKEGKR
                     EQLATVLYYALESLRLAADLYAPALPNKCAELKKALGESQPTGAPFADGPQWGLLKPG
                     TKIKLTSSLFPRLEQEKVKMQTMADDDQKNQVTIDEFGKLDLRVAQVTSAQKVTGADK
                     LLQLEVEVAGEKRQVIAGVAQHYAPEVLLGKKIVVVWNLKPAKIRGLESQGMLLAAQA
                     DGQLVLVTVDGEIKTGAKIS"
     gene            complement(2009..2863)
                     /locus_tag="AC16FU_10930"
     CDS             complement(2009..2863)
                     /locus_tag="AC16FU_10930"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR2994922.1"
                     /translation="MFVESFITEFKGSRREYCTNPQIIPLRPGDYVIMQAERGEDMGQ
                     FTKRVLFEKAPHGRHSTLDFYPILRKGKREEIERLKHNREREKESLKECKRLVIEYGL
                     QMKVVDVEYQFDSSKITFYFTADRRVDFRALVRELAAIYRTRVELRQIGARDEARRIG
                     GFGRCGLKQCCTTFINRDFPQLTTQLARDQYLPTNPSRITGNCGRLLCCLSYERDTYL
                     AAAELFPRIGAKYQTPKGKGEVVKIDALKMQMAVKLESGEEEVVTALDLRRQKQKEKG
                     LFSLVTAG"
     gene            3002..3796
                     /locus_tag="AC16FU_10935"
     CDS             3002..3796
                     /locus_tag="AC16FU_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994923.1"
                     /translation="MFFSFKQRSLSLKRLFIFLGALSLLLFFGRCSAKKKIFTPALLP
                     VDTPFALVIDAVQATPGTQGVAVEIRNNKSSYDSAIADGGRMGFFNFLVGYDPLVLQL
                     SKVEAGQILKEQRWDFFTYRFGPGNPPCSSCPSSWIRIIAVADINDSVPPTIFDGRNA
                     GGWFTMFFNVSTDSAFCSSFSPIRWIWWSCGDNYVTDSSEKRLWVVDTLRTVTSEIVN
                     VNSTFLIDSSDCNQFYSHWPIRRKFLNFRDGGVRIVCPPVEVAKRR"
     gene            3912..>4787
                     /locus_tag="AC16FU_10940"
     CDS             3912..>4787
                     /locus_tag="AC16FU_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR2994924.1"
                     /translation="MTDQQLRREIFQLAWPIVLSNLLQRGVGIVDTLLVGHVGANELA
                     AVGLCQVLIMVAVALLYAVGTGTNVLVAFHTGANEIKQRREVANTSLWVGFVLSILIS
                     LVGYQLSATGARVMGGTGTVVELAVTYLSITWIFFIFKAYINILSNIFQGTGDTRTPL
                     WVIIGVNVLHVLIAYPLIFGVWGFPRWGVRGAALASGISEALGALVLLAIAIQRKLVA
                     SHFVWYRKEDVRRLVSVGSPVAGERLLIQSMQIVYARLLLGFSVATYAAHQIGLAIES
                     FSFLPGLGFSQAATTL"
BASE COUNT          922 a         1288 c         1339 g         1238 t
ORIGIN      
        1 cgatcatgcg aaaaccaaaa acgtcctgcc taaggcggga cgtccgtacc gggacgatac
       61 catgaggggg tcagctaatc ttcgcaccag ttttgatttc cccatcgacg gtcaccagca
      121 ccagttggcc gtcggcctgg gcggccaaaa gcattccctg cgactccaga ccccggattt
      181 tggccggttt caagttccag acaaccacaa tttttttccc caagagcact tccggtgcgt
      241 agtgctgcgc cacgccggca atgacctggc gtttttcacc ggctacttcc acctcaagtt
      301 ggagcagttt atcggcgccg gtcacctttt gagcgctggt gacctgcgcc acccgcagat
      361 cgagtttgcc gaattcgtca atggtcacct gattcttctg gtcgtcatca gccatggttt
      421 gcatctttac tttctcctgt tccagccgcg ggaaaagcga actggttagt tttattttcg
      481 ttcctggttt gaggagaccc cattgcggtc cgtcggcgaa gggagcgccg gtcggctggg
      541 attcacccag cgcttttttc aactccgcgc acttgtttgg gagcgccggt gcgtagagat
      601 cggccgccag gcgcaaactc tccagcgcgt agtaaagtac cgtggcgagt tgctcccgct
      661 tgccctcttt ggccagtgcc cagggggcct gtgtctccat gtagcggttg gcctctttta
      721 ccagtccgct ggtgtaggcg agggcgtcgt tgtaaacgta gcgcccattt tcgcccgcaa
      781 agccgacata cgcggtcaat gttttttccg ctaagttcag ggcaattttt tggaagtcct
      841 tatcttcatg cgtcggtttg cccggctgcg gaacctctcc attgaagtgg tcggccacta
      901 acttgaagac ccggctggcc aggttgccga aatcattcgc taagtcggcg ttgtactttt
      961 cggtgaagcg ttcggcctga atgtcgccat ccacgccgaa agggaactgc gtcaagagca
     1021 aaaaacgcgt gccgtccggg ccgtagcggg agaccaagtg gtgggggtcg atgacgttcc
     1081 ccaaactctt gctcatcttc tgcccgttga cggagaaaaa gccgtgaatg aaaagaaatt
     1141 ccgggggttt gatgcccgcg gccagaagga gcgcgggcca gtagatggca tggaacttca
     1201 aaatctcctt ggccatcaga tgaaccactt tcgcctggtt ccaccaggta tcgaactgtt
     1261 tggcgtcgtc gccgtagccg atggcggaaa ggtaattcaa gagcgcatcg acccagacgt
     1321 aggcggtttg cttcgggtca aacggcagcg gaatgcccca ggtgaccttc tcccgcgaga
     1381 gcgaaaaatc ttccagcccc tgtttgaata gtccgagcgt ctcgcgcttg cgttcttcag
     1441 gccggataca gatttgatcc gtttcaattc gcctctggac ttcgtctaaa tacgcagaaa
     1501 gacggaagaa atagttcttc tccgaaacga gttgcggttt ggtctggtgc agaggacaga
     1561 ggccgttcac cagctctttt tcggtcaaga atttttcgca cccgaggcag tagagaccct
     1621 ggtacttccc ctcgtaaatt atcggttcgc ctttgggcgt ccgcgattcg tgcaacttca
     1681 gcaaaaattt ttccactgcc acctgatgcc gtttatcggt ggtgcggacg aagtaatctt
     1741 cttttatgga aagggcctgc caggtccggc ggtataaacc ggcaatccgg tcgcagtgct
     1801 cctgcggtga aaggccggct tttttggctg ctgcggccac gttggcgccg tgctcgtcgg
     1861 tgccggtaag gaagaaaatt tcatccccgc gcgcttggtg gaaacgggtc aaaaggtcag
     1921 ccacgatggt cgtgtaggcg tggccgatgt ggggctcggc gttgacgtag tagatgggcg
     1981 tggtcaaaaa gatgcggttt ggcatacctc acccggccgt taccaaactg aaaaggcctt
     2041 tttctttttg tttctggcgc cggagatcga gcgcggtcac cacttcttct tcccccgact
     2101 ccagtttgac cgccatttgc attttcaggg cgtcgatttt gacgacttcg cccttccctt
     2161 tgggggtctg gtacttggcg ccgatgcgcg ggaagagttc cgccgcggct aagtaggtat
     2221 ctctctcata cgaaaggcag cagagcagcc ggccgcagtt gccggtgatc cggctggggt
     2281 tggtaggcag gtactggtcg cgggccaact gggtggtgag ctgggggaaa tccctgttga
     2341 tgaacgtggt gcagcactgc ttgagtccgc agcggccaaa gccgccgatc cgccgggcct
     2401 cgtcgcgggc gccgatttgg cgcaactcca cccgcgtccg gtagatcgcc gccagttcgc
     2461 gcaccagggc ccggaaatcg acccgccggt cggcggtgaa gtagaaggtg atcttcgagg
     2521 agtcgaactg gtattccaca tcgaccacct tcatctgcag accgtactcg atcacgagtc
     2581 gtttgcactc cttgagcgat tccttctccc gttcgcggtt gtgtttgagc cgttcgattt
     2641 cctcccgctt ccctttgcgc aaaatgggat agaaatccag ggtcgaatgg cggccgtggg
     2701 gcgctttttc gaacaggacg cgcttggtaa actgccccat atcttcgccc cgctcggcct
     2761 gcataatgac gtaatccccc ggccggaggg gtatgatctg gggattggtg cagtactccc
     2821 gccgcgagcc cttgaattcg gtgatgaaag attcgacgaa catagacgcc tttcgtctat
     2881 aatcaccgtt tcgaagcggg aagtcaaggc gtggcttgct tagattttgc ttgacagccc
     2941 acattcttcc tatatcttcg gagaagaggg tagtgcccgg cataaagagg agttgttttt
     3001 agtgtttttt tcattcaagc aacgtagttt atctttgaaa agattattca tctttttggg
     3061 agctctcagt ctccttctct tttttggccg ctgctctgct aagaagaaaa tttttacccc
     3121 ggcacttttg ccggttgata ccccgtttgc attggtaatt gatgcggtac aagcgacacc
     3181 cggtactcag ggtgtggcag ttgaaattcg caataataag agttcatatg actccgccat
     3241 tgccgacgga ggaaggatgg gcttttttaa tttcctagtc ggatatgatc cattagtgct
     3301 acaactctca aaagttgagg cgggtcaaat tcttaaagaa cagcggtggg atttctttac
     3361 ttatcgattc ggacccggta accctccgtg cagttcctgt ccgagttctt ggatacgaat
     3421 aatcgctgtt gccgacatta atgattcggt acccccgaca atttttgacg gacgtaatgc
     3481 gggcggttgg ttcactatgt tttttaacgt gagcactgat tccgcgtttt gtagttcttt
     3541 ttctccaata cgctggattt ggtggtcttg cggtgataac tacgtgactg attcgagtga
     3601 aaagcggctc tgggtagtgg atacgcttcg taccgtcacg agtgaaatcg tcaatgtgaa
     3661 cagtaccttt cttatagata gttccgattg taaccaattt tactcgcact ggccgattcg
     3721 gcgcaaattc cttaactttc gcgatggtgg tgtacgcatc gtttgccctc cggttgaagt
     3781 cgctaaaagg cgttgagtct accttaaaat accaactcca atagtctctg accggctcag
     3841 ggacgagccg gtctaccatt agtatttgtc aggtttgact ttcgcttaac tccctctatt
     3901 atccgaatct tgtgaccgat cagcaactgc ggcgggaaat tttccagctg gcctggccga
     3961 ttgtgctctc caatctgctc cagcgaggcg tcggtatcgt cgatacgctt ttagtcgggc
     4021 acgtcggcgc caatgaatta gccgcggtgg ggctctgcca ggttttgatc atggtggcgg
     4081 tggccttgct ctatgcggtg ggcacgggga cgaacgttct ggtcgccttc cacaccgggg
     4141 ccaacgaaat caaacaacgg cgcgaagtag ccaacacctc cctctgggtc gggtttgtcc
     4201 tctcgatcct catttctctg gtgggctacc agttgagcgc caccggcgca cgggtgatgg
     4261 gcgggaccgg caccgtggtc gagttggcgg tcacctacct ttccatcact tggatttttt
     4321 tcatcttcaa agcctacatc aacatcctct cgaacatttt tcagggcacc ggcgacacgc
     4381 ggacgccttt gtgggtcatc atcggagtaa acgtcctcca cgtgctgatt gcatatccat
     4441 tgatcttcgg tgtttgggga tttccccgct ggggggtacg cggcgcggct ttggccagcg
     4501 gcatttccga agcgctcggt gctctagtgc tcctggcaat tgccatccag cggaaattag
     4561 tcgccagcca cttcgtctgg taccggaagg aagatgtgcg ccggctggtg agcgtcggct
     4621 ctcccgtggc gggggagcgg cttttgattc aatcgatgca aatagtctat gcccggctgc
     4681 ttttgggatt ttcggtcgcc acttacgcgg cgcaccagat tggattggcc atagagtcgt
     4741 tctcgtttct gcccggtctc ggtttctctc aggcggcgac cactctc
//
LOCUS       DBNGZA010000515         4151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1585857, whole genome shotgun sequence.
ACCESSION   DBNGZA010000515 DBNGZA010000000
VERSION     DBNGZA010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1585857"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16FU_10945"
     CDS             <1..403
                     /locus_tag="AC16FU_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR2994925.1"
                     /translation="RKVRFATEVAPGLPKVAMDVGQIQQVLLNLFNNAAEAMAERNPP
                     GGTITIEVKLLPEKSQVEIVVADDGPGIQPAYLDRIFDPRFTTKKTGHGLGLSTCKRI
                     IENHRGTITVKSAPGVGTQFFIRLPLSQTQT"
     gene            419..>4151
                     /locus_tag="AC16FU_10950"
     CDS             419..>4151
                     /locus_tag="AC16FU_10950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR2994926.1"
                     /translation="MSRTTQVQPQPVVQKRVLSSRPRFLAGCRFRILATLGEGGFGAV
                     FGCYDRLRQKRVALKTCASRGAPGRPEANVKAGERFRREFHLARTLSHPGVVRAFDFG
                     VLDSGELYYTMDWCAGKPLSSFAPLEKLDFLPDLIRKICGVLEFLHAFHIVHSDLKSS
                     NILVRFDPQAPQGPWCDLKLMDFGLAEEFTFGAPQTQAGTLEYMAPEMIEGKGGDARA
                     DLYSLGCVLYELVTGRTPFASVDPLSVVEGHLAKAPASPAELRSELSPLLNDLILKLL
                     AKNPAERMQSAREVAAQVVPIPVAIRAPEPFAALRNYMESGLALALGKNTPALVTASA
                     KRGVVGIAYGGRTTILGPPPSDTLEMQLQAQGFATVTLDAAVPQPEIARALVKKLWLV
                     PGAHDGQRSAPQPTHLRHWLRVSEAGRGDAGFSERALEELTNWITAQMSAAGKISPPA
                     VIVQNVSQGFLETAVYRKVLTALAHSGHCLVILCPGKSERYADDRRLWRGLNSGATAE
                     TWEFPRMGGGDFNRSTLVPETVIATSDLSRLWEIFGREENIFAAGLRFLHEKNFLESC
                     DGLWRVNRQKMEGFRLPRPLRRSLIRQVSHLNPEQCALLRAGSIFSGPFTALGVARLT
                     GLTRPVAEKQVATLQKERVLNLVTGSETSVQHYGFGSALLRRALYESISASQRRAGHR
                     KVLKFLISIPDSTPEGQKSLADHALASDDATEAVTRVLAYAGLCVEKNEREETLLYFA
                     RAERKARRVRESNLRKQLLARVLFERADRYKGWGDFEPAEKDFRIVLSLIPVERDPSL
                     HAEVYRKLADLYRWQNRLDDAGRTIEQAIALLQKTGDRVALSRAYNNLGIITWRKGDV
                     PQSVAYYETAYQIQKEQGLAQEAILTLNNLGIAYIQTHRFSTALKHYAEALELGRREG
                     TPETIAHLLNNIGFTHASVGSLAEGKPYFTESLSLRRALGKKSEILLTLINLSWLAYL
                     TGDPDGLKQLGQEAIALAQEVDDPQSVAEASFVLAEESLREGDYAGAYALVQAPQMDA
                     VDTFISNPVARAAFWVRFYAGQGDCEGAESAAARAKEVAERSGDLRNKVLSQIAGLTL
                     ALAAENGWEESAALLREAERTIEPFEGLLEKSQLQLCKVRHALRSGAPALLPDELAKL
                     DTLLAQCESAPLLAERFFLEGKWRSRRRDFLKATQAFTQANNFAGQAGYRELLWRIGT
                     EAGRVAATIGDYETAFREYRSAYEIVKVIYSCLSDPAYQV"
BASE COUNT          869 a         1227 c         1283 g          772 t
ORIGIN      
        1 ccggaaagtc cgtttcgcca ccgaggtcgc tccggggttg cccaaggtgg cgatggacgt
       61 ggggcagatc cagcaggtgc ttttgaatct cttcaacaac gcggcggaag cgatggcaga
      121 gcgcaacccg cccggaggga ccatcaccat cgaggtgaaa cttttgccgg agaagagcca
      181 ggtggaaatt gtggtggctg acgacgggcc gggcattcag cccgcgtacc tggaccggat
      241 tttcgaccca cgcttcacca ccaagaaaac cggccacggc ctggggcttt ccacctgcaa
      301 acggatcatc gagaatcacc gggggacgat cacggtcaaa agcgcccccg gcgtcgggac
      361 gcagtttttc atccggctgc cgctctcgca gacgcagacc taattttggg acaggggaat
      421 gtcccgtaca actcaagtcc aaccgcaacc ggtcgtccaa aagcgcgtgc tttccagccg
      481 gccacgcttt ctcgctgggt gtcgctttcg gatactcgcc acgctgggag aaggtggttt
      541 cggtgcggtc tttggctgct acgaccggct ccgacaaaag cgggtggcgc tgaaaacctg
      601 cgccagccgg ggagcgcccg gccggccaga ggcgaacgtc aaagccgggg agcgcttccg
      661 gcgggaattc cacttagccc gcacactttc gcatcccggt gtggtgcggg cctttgattt
      721 cggcgtgcta gactccggcg agctctacta taccatggac tggtgcgccg gaaagccgct
      781 ttcctctttc gcgccgcttg agaaattaga ttttctgccg gatttgatcc ggaaaatctg
      841 cggcgtcctg gagttcttgc atgcgttcca catcgtccac tccgacctga agtccagcaa
      901 catcctggtc cgattcgatc cgcaggcccc gcaggggccc tggtgtgatc tcaaactgat
      961 ggatttcggc ctggccgagg aattcacttt tggcgcgccg caaacgcagg cgggaactct
     1021 ggagtacatg gcgccggaga tgatcgaggg taaaggcggc gatgcgcggg ccgacctcta
     1081 ctctctgggc tgcgtgctct acgaactggt cacggggcgg acgccttttg cgagcgtcga
     1141 tccgctctca gtcgttgaag gacatctggc caaggccccg gcgtctccgg cggagttacg
     1201 ttctgaactt tctcctctgc tcaatgactt gattcttaaa cttttggcca aaaatccggc
     1261 cgagcgcatg caatcggccc gcgaagtcgc agcgcaggtg gtgccgatcc cagtagccat
     1321 cagggcaccg gaacccttcg cggcgttgag gaattatatg gaaagcggcc tggctctagc
     1381 actgggtaaa aacacgccag cgctcgtcac cgcttccgcc aaacggggtg tcgtaggcat
     1441 cgcctacggt ggccggacaa cgattctggg gccgccgcct agcgatacgc ttgagatgca
     1501 gctgcaagcc cagggttttg caactgtcac actcgacgcc gcagtgccac agccagaaat
     1561 cgcaagggcg ctggtcaaaa agctctggct cgtccccggc gcacacgacg gacaacggag
     1621 cgcaccccaa cccacacacc tgcggcactg gctccgggtg tccgaggcag gaaggggaga
     1681 tgccggtttt tcagaacggg cgttggaaga actgacgaac tggattaccg ctcaaatgtc
     1741 cgctgcgggt aagatttccc ctcccgcggt catagtccag aatgtgtcgc aaggattcct
     1801 cgaaacagca gtataccgca aggtgctcac agcgctcgct cattcgggac attgcctggt
     1861 catcctctgc ccggggaaaa gcgaacggta tgccgatgac cggcgactct ggagggggtt
     1921 gaatagtggg gcaactgccg agacctggga gttcccccga atgggcggcg gtgattttaa
     1981 ccgatcaaca ctggttcccg aaacggtcat tgccacgagt gatttatccc ggttgtggga
     2041 aatctttggc agagaggaaa atattttcgc agcggggctg cgcttcttgc acgagaaaaa
     2101 ctttctggag tcgtgcgacg gactatggcg ggtcaaccgg caaaagatgg agggctttcg
     2161 cttaccccgt ccactgcggc gcagtctgat acggcaagtc agccacctga acccggagca
     2221 gtgtgcgctc ctccgcgccg ggtcaatttt cagtggaccc ttcactgccc ttggcgtcgc
     2281 ccggctcacc ggcctgacgc ggccggtggc ggaaaagcag gtggcaacac tccaaaaaga
     2341 gcgggtcctg aacctggtga ctggaagcga gaccagtgtt caacactacg gcttcgggtc
     2401 agcacttttg cgccgggcgc tgtacgaaag catttcggcg agtcaaagac gggccgggca
     2461 ccgcaaggtg ctcaaattcc tgatcagcat ccccgattca actcccgaag ggcaaaaaag
     2521 cctggccgac cacgcgctgg cgagcgacga cgcgacagaa gccgtcaccc gggtgctcgc
     2581 ctacgccgga ctgtgcgtgg aaaaaaatga gcgcgaggaa acgctcctct acttcgcccg
     2641 tgcggagcgc aaggcgcggc gggtgcggga gtcaaacctg cggaagcagc tgctcgcccg
     2701 ggtgcttttc gagcgggccg accgctacaa ggggtggggc gattttgaac cggcggaaaa
     2761 agatttccgc atcgtactct ccctgattcc ggttgagcgg gacccgtcgc tccacgctga
     2821 agtctaccga aagctggccg acctctaccg ctggcagaat cggctggacg acgccggccg
     2881 gacgattgag caagcgattg cgctcttaca aaagaccggc gacagggtgg cgctttcccg
     2941 ggcgtacaac aacctcggca tcatcacctg gcgcaaggga gatgtcccgc agtcggtggc
     3001 gtactacgaa acggcctacc agatacagaa agaacaaggt ctggcgcaag aagcgatttt
     3061 gaccctgaac aatctgggga tcgcctacat tcagacgcac cggttctcca ccgccctcaa
     3121 acactatgcg gaagcgctgg aattaggacg ccgagagggc acgccggaaa ccatcgccca
     3181 cctcttgaat aacatcggtt ttacccacgc gtcggtcggc tcattggcgg aggggaaacc
     3241 gtacttcaca gaatcgcttt cgctccggcg cgccttgggg aagaaaagtg aaatactctt
     3301 aaccctgatc aatctcagct ggctggccta ccttaccggc gatccggatg gactcaaaca
     3361 actggggcaa gaagcaatcg ccttagccca agaagtggat gatccacagt cggtggcgga
     3421 ggcgtcattc gttctggcgg aggagtccct gcgcgaaggc gactacgccg gagcgtatgc
     3481 tttggtccaa gcaccacaga tggacgcggt ggacacgttc atctccaacc cggtggcgcg
     3541 ggctgccttc tgggtgcgtt tttacgccgg acagggtgat tgcgaagggg cggagagcgc
     3601 ggcggcaagg gcgaaagagg ttgcggagcg atccggcgac ctccggaata aagtcttaag
     3661 ccagatcgcc gggctcacgc tggctctggc cgccgaaaat ggttgggaag agagcgctgc
     3721 gctcctgcgt gaagcggaac gaacgatcga accgttcgag ggattgctcg agaagagtca
     3781 actccagtta tgcaaggtcc gccacgcgct ccgctccggc gcaccggcgc tcctgccgga
     3841 tgaattggca aaattggaca ctttgttagc gcagtgcgag tcagctccgc ttctggcgga
     3901 acgtttcttt ctggaaggga agtggcgcag ccggcgacgg gatttcctta aggcgaccca
     3961 ggccttcact caggcaaata atttcgccgg gcaagcaggg tatcgggaac tgctctggcg
     4021 catcggaaca gaggcgggcc gcgttgccgc caccatcggg gattacgaaa ccgccttccg
     4081 cgaataccgg tcggcctatg aaatcgttaa ggtgatctac agctgccttt ccgacccggc
     4141 ctaccaggtc c
//
LOCUS       DBNGZA010000516         4668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_2648313, whole genome shotgun sequence.
ACCESSION   DBNGZA010000516 DBNGZA010000000
VERSION     DBNGZA010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_2648313"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      207..332
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            417..1520
                     /gene="ribD"
                     /locus_tag="AC16FU_10955"
     CDS             417..1520
                     /gene="ribD"
                     /locus_tag="AC16FU_10955"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR2994927.1"
                     /translation="MRQTFALAQKGWGYTSPNPLVGAVVVKNGRIMGQGYHHKAGEAH
                     AEVIALAAAGKKARGATLFVNLEPCCHYGRTAPCTEAILSAGVKKVVLSVIDPNPLVN
                     GKGIRTLERHKVAVVTGVLEDAARQLNEVHFKFITTGLPFVIIKVAQSWDGRLATLTG
                     DSQWISGEKARRFAHLLRSRVDAVLVGRDTVAADNPRLTVRLVKGNNPYRIVLDSVGR
                     VLPQSRLVKENQDGKTIWTRAKNGQATIDAMKGVKIWELPAGENGWIDLPAFLRQAGR
                     EEITSLLVEGGSRVVTSFLRQQLADKIYFCFGPLILGEGKDTVGDLNVRRLSEGIRLR
                     EVGMRKLAQDILVWGYPAWPDGSSQPKNRPSEN"
     gene            1524..2210
                     /locus_tag="AC16FU_10960"
     CDS             1524..2210
                     /locus_tag="AC16FU_10960"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR2994928.1"
                     /translation="MFTGIVREIGRVEQVARQGGGMRLTICSTSVRQSLAVGDSVSID
                     GVCLTVATLTPKGFQADVIPETLRKTQMGSLSPNERVNLEPALRIGDMLGGHFVTGHI
                     DCTGTVTELVEAGADRLLAVQFPTDFMTWVIPQGSIAVDGVSLTVAAVLACGIRVALI
                     PHTLENTALGALKSGERVNLEFDLLGKYAVRWSQARIAPSRISEPWLQEIGWSVPRLP
                     TVFSRNQEER"
     gene            2207..3442
                     /locus_tag="AC16FU_10965"
     CDS             2207..3442
                     /locus_tag="AC16FU_10965"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR2994929.1"
                     /translation="MNYHTIEEALADIRAGKMVIVDDESREDEGDLIMAAEKITPEAV
                     NFMAQHGRGLICVALTADRARALNLPQMVAENTAKLETRFTVSVDALPNTQSGCSAFD
                     RAQTIRLLADPQTRPDDLARPGHIFPVVAAEGGVLKRPGHTEAAVDLARLAGLAPVGV
                     ICEIMNDAGKMARGRELFALAEKFSLKVVTIADLVTHRCKTEKLVQRVSTVDFPTRFG
                     HFHLHLYRALVEKQDHLAIGKGAVEGKSGVLVRVHSQCMTGDIFHSSRCDCGAQLEQA
                     LEHVEKEGSGVLVYLCQEGRGIGLANKILAYSLQDQGQDTVEANVALGFEPDLRDYDV
                     AAQILRDLGVQSVRLVTNNPHKVADLKRYGVPVEQRLASITEPTAHNRRYLRTKKEKM
                     GHWVEVEGRALTGEPVKKK"
     gene            3439..3918
                     /gene="ribH"
                     /locus_tag="AC16FU_10970"
     CDS             3439..3918
                     /gene="ribH"
                     /locus_tag="AC16FU_10970"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR2994930.1"
                     /translation="MKEHKGQLSASGKKFAIVASRFNEFVTHQLVSGCLDCLTRHGAD
                     DQSIELFWVPGAFEIPPVAQRLATRKKYDAIICLGAVIRGGTPHFTFIAAAATNGIAA
                     VAQHSALPVVNGVLTTDNLEQAIERAGAKAGNKGWDAGLAAIELINLYAELEHGRKT"
     gene            3902..4336
                     /gene="nusB"
                     /locus_tag="AC16FU_10975"
     CDS             3902..4336
                     /gene="nusB"
                     /locus_tag="AC16FU_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR2994931.1"
                     /translation="MGARRKAREVALGCLYAAETATVAPQNVLTLHLAREKLSAKNRA
                     FVTVLFEQVRANTKTCDGLIARHLKHWDIARLSLIDRLVMHLAICEFLFFPDIPFRVT
                     INEAVELAKKFSTGDSGAFVNGVLDAIAKTIEQKTPAGTTAQ"
     gene            4402..>4668
                     /locus_tag="AC16FU_10980"
     CDS             4402..>4668
                     /locus_tag="AC16FU_10980"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR2994932.1"
                     /translation="MGTPAVVIARSPITLPLPVRDGISSKWVDRLIGALVFLASFIVF
                     YLTKAPTLSFWDCGEFVACAAILGIPHPPGSPLMVVLGRVFTLLP"
BASE COUNT         1008 a         1256 c         1455 g          949 t
ORIGIN      
        1 cgtgtttcac gcgctgtaac ccctgcacct gtcatattca gaaatgttgt gacggggtgt
       61 gcatcgattt ggcgcaacgg tgcaggttct gtccactcgt gatctgtgaa taggtggata
      121 gcgggacggt aaaaagtccc ccgcttcggc ggggggcttt ttgctttatg ggctattgac
      181 aaagccagca gattgcgtat actaataaag aacttcgggg cgaggtggaa ttcctcgatc
      241 ggtggtgaaa gcccacgact cccctaaaac gagggactga acccgtgaaa tccgggtgcc
      301 gacggtaaaa gtccggatgg aagaagttcg atatctgatt ggcggcttct ttctttgccc
      361 cgaagtccgg ttgttagagg aatcggggta tatcatttat ggacgtaaac ggcttcatgc
      421 gccaaacatt tgccctggct cagaaagggt ggggctacac cagccccaat ccgttggtgg
      481 gagccgtggt cgtgaaaaat ggccgaatca tggggcaggg atatcatcac aaagcgggag
      541 aggcgcatgc tgaggtcatt gctctggcag cggcggggaa aaaagcccga ggagccactc
      601 ttttcgtcaa tctcgaaccc tgctgccact acgggcgaac cgcaccctgc accgaggcaa
      661 tcctctccgc aggggtcaaa aaagtcgttt tgagtgtgat cgatcccaac cccttggtga
      721 acggaaaggg aatccgtaca ctcgagcgcc acaaagttgc agtagtaacc ggcgtcctgg
      781 aagatgcggc caggcagctc aacgaagttc acttcaaatt cattaccacc ggcctcccgt
      841 tcgtcataat caaagtcgcc cagtcgtggg acggtcggct ggcgaccttg acgggcgact
      901 cgcagtggat ttctggcgaa aaagcgcgcc gttttgccca tcttttgcgc agccgagtcg
      961 atgcggtact ggtaggccgt gacacggtgg ccgccgacaa tccccgcctg acggtgcggc
     1021 tggtgaaggg gaacaatccg taccgaattg tgctcgattc ggtcggccgg gtgctcccgc
     1081 aaagccggct cgtgaaggaa aatcaggacg gcaagacgat ctggacaaga gccaaaaacg
     1141 gccaggcaac aatcgatgcc atgaaaggtg tcaagatctg ggaattgccc gccggagaaa
     1201 acgggtggat cgatctaccc gcttttttgc ggcaagccgg acgggaagag atcaccagtc
     1261 ttctggtaga aggcgggagc cgcgtggtca cctcatttct ccggcaacag ctggcggata
     1321 aaatctactt ctgctttggg ccgctcattc tgggcgaggg gaaagacacg gtcggcgacc
     1381 tgaatgtccg gaggttgagc gaaggaattc ggctgcgtga agtgggaatg cggaaattag
     1441 ctcaggacat cctcgtctgg ggttatccgg cttggccgga cgggtcatct caaccgaaaa
     1501 atcggccgag cgagaattaa gcaatgttca ccggcattgt gagagaaatc ggcagagttg
     1561 agcaggtcgc ccggcagggg ggaggtatgc gtctcaccat ttgctccacc tcagtgcggc
     1621 agtcgctggc ggtcggcgat tcggtcagta ttgatggcgt ctgtctgacc gtcgctacgc
     1681 tcacacccaa gggctttcag gcggatgtca tcccggaaac gctgcggaaa acccaaatgg
     1741 gctcgcttag cccgaacgaa cgggtgaatc ttgaaccggc gttacgcatc ggagatatgc
     1801 tgggcgggca tttcgtgacc gggcacatcg actgcaccgg cacagtgact gaactcgtcg
     1861 aagccggagc ggatcggttg ctcgcggtgc aattccccac cgactttatg acctgggtta
     1921 tcccacaagg ttcgattgcc gtcgatggag tcagcctgac cgtggcagcg gtgctcgctt
     1981 gtggcatccg cgtggcgctc attccgcaca cgctggaaaa caccgccctg ggtgccttga
     2041 agtcaggcga acgggtgaat ctcgaatttg atcttctggg caagtacgca gtgcgctggt
     2101 cgcaagcacg gatcgctcct tctcggatca gcgagccatg gttgcaggag atcggctggt
     2161 cggttccgag gctgccaacg gtcttctcgc gaaatcagga ggaaagatga attaccatac
     2221 catagaagaa gcgctggccg acatccgcgc cgggaaaatg gtgatcgtgg atgacgaaag
     2281 ccgggaagat gaaggcgacc tgatcatggc ggcggaaaaa atcacgccgg aagcggtcaa
     2341 cttcatggcg cagcacggcc gcggattgat ctgcgtcgct ttgactgccg accgcgcccg
     2401 ggcactcaac ctgccgcaga tggtggccga aaacacggcg aaattggaaa cgcggttcac
     2461 cgtgtcggtc gatgcactcc ccaacaccca gagcggctgt tcggcctttg accgggcgca
     2521 gacgatccgg ctcttggctg atccccaaac gaggccggat gacttagccc ggccgggaca
     2581 catttttccg gtggtcgccg ccgagggcgg cgtactcaag cggcccgggc atacggaagc
     2641 ggcggtcgac ctggcgcgtc tggccgggtt ggctccggtg ggggtcatct gcgaaatcat
     2701 gaatgatgcc gggaaaatgg cgcgcggaag agagcttttc gctttggccg aaaaattcag
     2761 tttgaaggtt gtcacgatag ccgatctggt cacccaccgc tgtaagacgg agaagttggt
     2821 ccagagggtc tcgacggttg acttccccac ccgtttcggg catttccacc tgcacctgta
     2881 ccgggcgctg gtggaaaagc aggatcacct ggcgataggg aaaggcgcgg tagagggaaa
     2941 aagcggggta ctggtgcggg tgcattcgca atgcatgacc ggcgacatct tccattccag
     3001 ccgctgcgac tgtggcgcac aactggagca ggcgctggaa catgttgaaa aagagggctc
     3061 aggggttctg gtttatctct gccaggaggg acgaggcatc gggctggcca ataaaatttt
     3121 agcctacagc ctgcaggatc aggggcaaga cacggtggag gccaacgtgg cgttgggttt
     3181 tgaacccgac ctccgcgact acgatgtggc ggcgcaaatt ctccgcgacc tgggagtgca
     3241 gtcggtgcgt ttagtcacaa acaacccgca caaagtggcc gacctcaagc gctacggtgt
     3301 gccggtggag cagcggctgg cttcgatcac ggagccgacc gcacacaacc ggcgctacct
     3361 cagaaccaaa aaagaaaaga tgggccactg ggttgaggtg gaaggccgcg ccctgaccgg
     3421 cgaaccggtg aagaaaaagt gaaagagcac aaaggacagc tgagtgcttc cggcaaaaag
     3481 ttcgcgatcg tcgccagtcg gttcaacgaa tttgtaacgc accagctcgt cagcggctgc
     3541 ctggactgcc tcacccggca cggagccgac gaccagagta tcgagttgtt ctgggtcccg
     3601 ggagcttttg agattcctcc cgtggcacaa cgcttggcca cacgcaagaa atatgacgcg
     3661 atcatctgtc tcggggcggt catccggggc gggacgccac atttcacgtt tatcgcggcg
     3721 gcggcgacca atggcatagc cgctgtggcg caacattcag ctctaccggt ggttaatggc
     3781 gtcctgacca cagacaattt agagcaggcg attgagcggg ccggggccaa agccggcaac
     3841 aaaggctggg atgcggggct ggcggcaatt gaactgatca acctctacgc tgagctggag
     3901 catgggcgca agacgtaaag cgcgggaagt ggccttgggt tgcctctacg cggcggagac
     3961 ggcgaccgtg gcgcctcaga acgttctcac gctccatttg gcccgcgaga agctttctgc
     4021 caagaaccgg gcttttgtaa ccgttctctt tgaacaagtg cgggccaaca ccaaaacgtg
     4081 cgacggcttg atcgcccgac atctgaagca ctgggatatt gcccgtcttt cgctcatcga
     4141 ccggttggtc atgcacctgg cgatctgcga atttctcttc ttccccgaca tccccttccg
     4201 ggtgaccata aacgaagcag ttgagttggc caagaaattt tccacgggcg attcgggcgc
     4261 tttcgtcaac ggcgtcttag acgccatagc caaaaccata gagcagaaga ctccggccgg
     4321 aacgaccgct caatagctac tttttttcga gggggaaatg gcgttgagta agctccgttc
     4381 gggctgtata ttcgccaaac tgtgggaacg ccagccgtcg tcatagcccg ttcacctatc
     4441 acactcccac ttccggttcg tgatgggatc tcttccaaat gggttgaccg tttgatcggt
     4501 gctttggttt tcctggccag ttttattgtt ttttacctga cgaaggcgcc gaccctgtcg
     4561 ttttgggact gcggggagtt tgtggcctgc gcggcgatct tggggattcc gcacccgcca
     4621 gggtcgcctt tgatggtggt tttggggcgg gtgttcacgc ttttgcct
//
LOCUS       DBNGZA010000517         1962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1904968, whole genome shotgun sequence.
ACCESSION   DBNGZA010000517 DBNGZA010000000
VERSION     DBNGZA010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1904968"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="AC16FU_10985"
     CDS             complement(<1..619)
                     /locus_tag="AC16FU_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR2994933.1"
                     /translation="MYPSLRSDLVESRQESGGVRYVVVKDPVTLRFFRLREPEYFIAT
                     QLDGKTPPEEIGERLKVKFGIDLPAAAVAEFISRLEKAGFTEGSLSEHQIARARDQRP
                     DQGKPSLLKRLVFIKLKAFDPDNFLTRLAPYTNIFYTKGFVILSGALFLVAANITLAN
                     KEAFLAVHLGDLFRVGGILFAVFTIFLVIALHELAHAVTCKRYGGR"
     gene            complement(637..1833)
                     /locus_tag="AC16FU_10990"
     CDS             complement(637..1833)
                     /locus_tag="AC16FU_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR2994934.1"
                     /translation="MEHLTRILFELFAIFLAAKLAGEIFERLHQPAVIGEILAGILLG
                     PHLLGLVNPSEILDVFAEIGVIILLFTIGLQTKVEDLLRVGWISLFVGVLGIALPFIL
                     GFALLKALNYKTVEALFVGTAMVATSVGITARVFADIGVLKERTSQIILGAAVIDDIL
                     GMLFLAVVSGLGEGTISYVHLGIVFLEAAGFVLFLIFVGRRLIRRVVPEMNQFKSRNA
                     PFSLAILFCLGLSAMANYLQIAAIVGAFMAGMVLAEFNLVYGISVKSESLYDFLVPFF
                     FVVMGTKVDLQVFLQGQGLGLALGITLLAIVGKLVGCGLAASGLGFRSALTVGVGMVP
                     RGEVGIIVAKIGLSLHTIGTNLYAVVIFMVLATTLSTPPFLRRLAVAATRKSEVPTGG
                     GLPEAE"
BASE COUNT          462 a          555 c          576 g          369 t
ORIGIN      
        1 cccggccgcc gtagcgtttg caggtgaccg cgtgggccag ttcgtgcagg gcgattacca
       61 aaaaaatggt gaaaacggca aagagaatac cgcccacccg gaagagatcg cccaggtgga
      121 ctgcgagaaa agcttctttg ttcgccaggg tgatgtttgc ggcgaccagg aagagcgctc
      181 ccgaaagaat gacaaaacct ttggtataaa aaatgttggt gtagggggcg agccgggtta
      241 agaaattgtc cggatcgaag gccttgagct tgatgaaaac caaccgtttc agaagcgacg
      301 gtttgccttg atccggacgc tggtctctgg cccgggcaat ctggtgttcg ctcaagctcc
      361 cttcggtgaa tccggctttt tccaatcggc tgatgaactc ggccaccgcc gcggcgggca
      421 ggtcaatccc gaattttact ttgagtcttt ctcctatttc ttccggtgga gtctttccgt
      481 ccagctgggt ggcgatgaag tattccggtt cgcgcagccg gaagaagcgg agggtcaccg
      541 ggtctttcac gaccacatag cgaactccgc ccgattcctg gcggctttcg acaaggtcgg
      601 accgaagaga gggatacatc aatcacgtcc tctaagctat tcggcctcgg gcagaccgcc
      661 gccggttggc acctcacttt tcctggttgc ggctaccgcc agccgccgca ggaagggcgg
      721 tgttgagagc gtggtggcca gcaccatgaa gatcaccacc gcatagagat tcgtcccgat
      781 ggtgtggagc gaaagcccga ttttggcgac gatgatcccc acctcgccgc ggggcaccat
      841 cccgacgccg acggtcaagg cgctgcgaaa tcctaaaccg ctggccgcca gaccgcagcc
      901 gactaacttc cccacaattg ctaagagtgt tatccccagc gccagaccga ggccttgccc
      961 ttgcaggaag acctggaggt caactttggt gcccatgacc acgaagaaga aggggaccag
     1021 gaagtcgtag agcgattccg acttcaccga gatgccgtag accaagttga attcggccaa
     1081 gaccatcccg gccataaagg cgccgacgat ggcggcgatt tgcaggtagt tggccatggc
     1141 cgaaagcccc aggcagaaga gaatggcgag cgaaaagggg gcgttgcggc ttttgaactg
     1201 attcatctcc ggcaccaccc ggcgtatcag gcgccggccg acgaagatca agaacaagac
     1261 aaatcccgcc gcttccagaa agacgattcc gagatggacg taggagatcg ttccctcacc
     1321 cagtccggag accacggcta agaaaagcat ccccagaatg tcatcgatca ccgctgcgcc
     1381 caggatgatc tggctggtgc gctctttgag cactcctatg tcggcaaaaa cccgggcggt
     1441 gatgcccacc gaggtggcga ccatagccgt gcccacaaag agggcttcta cggttttgta
     1501 gttcagcgct ttcaggagcg caaaacccag aatgaaaggg agggcaattc ccagcacgcc
     1561 gacgaagagc gaaatccagc ccacccgcag gaggtcttcg accttcgtct gcaggccgat
     1621 ggtaaacagc aaaatgatga cgccgatttc ggcgaacacg tccaagattt ccgaaggatt
     1681 tacgagcccc aggaggtggg ggcccaaaag gatgccggct aagatttcgc ctattaccgc
     1741 cggctggtgc aggcgctcga agatctcgcc ggccagtttg gccgccagga aaatggcgaa
     1801 gagttcaaaa aggatgcgcg ttagatgttc cataccccca cggagtgaac ataccgtccg
     1861 gtgaattgaa tggtcaagga aatttgttcg gtattaaacg accggaagtt ctcctgcatc
     1921 ggatgactcc aacacccgtt ggacgcaccg tgcgttcggc cg
//
LOCUS       DBNGZA010000518         3726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_528304, whole genome shotgun sequence.
ACCESSION   DBNGZA010000518 DBNGZA010000000
VERSION     DBNGZA010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_528304"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16FU_10995"
     CDS             <1..374
                     /locus_tag="AC16FU_10995"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR2994935.1"
                     /translation="DLDGAFTAYNLYRDRYPDGLGLPRLQEKLRAVHDTGAVEELPDG
                     GAQKRWSLQAGVFGNLENAQRMLKRYQAAGYPVEMTSQVISGKKLFSIFIGSYATEAE
                     ARRKKAEIETKEKEVLKLVIR"
     gene            699..3221
                     /gene="mutS"
                     /locus_tag="AC16FU_11000"
                     /pseudo
     CDS             699..3221
                     /gene="mutS"
                     /locus_tag="AC16FU_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
     gene            3232..>3726
                     /locus_tag="AC16FU_11005"
     CDS             3232..>3726
                     /locus_tag="AC16FU_11005"
                     /inference="COORDINATES: protein motif:HMM:NF052691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR2994936.1"
                     /translation="MTKKIRSFFYTGKIPITFFRFAVILLPIQSVSAQNFWEQANGPY
                     SGWIQALAINSSGHFFAGTYGAGVFRSIDNGGSWTQTGLTVSTVWSLAINSSGAIFAG
                     TRGGVFRSTDNGNSWTQTGLADVYCLAINSSGQIFAGRANAGVFRSTDNGGSWTQTGL
                     MNTFV"
BASE COUNT          858 a         1024 c         1043 g          801 t
ORIGIN      
        1 gggatcttga cggcgccttc actgcctaca atctctatcg cgaccgctat cccgacggcc
       61 tggggctgcc gcgtttgcag gagaagctgc gggccgtgca cgacaccggt gcggttgaag
      121 aactccctga cggcggcgcg cagaagcgct ggtcgctcca ggccggagtc ttcgggaacc
      181 ttgaaaacgc gcagcgcatg ctgaagcgct atcaggccgc agggtatccc gtggaaatga
      241 cgagccaggt gatttccggc aaaaaacttt tctcgatttt catcggctcc tacgccaccg
      301 aggccgaagc acgcaggaag aaagccgaaa tcgaaacgaa ggaaaaagaa gttctgaagt
      361 tggtcatccg ctaattcgct cttcccattg aagggagacc cgtacaggtc ccctacgcgt
      421 ttttgggatc ctccttaccc tcccctccga ggggagggca gccatacggc ccggttgggg
      481 aggggtcgtt cagccaagtc gtatcgaccg cgcccacgcg cgtctgagct cgcccgcgta
      541 gggcgaccgc ccccggccgc cctttgtaga actgctcgat tgggagcacc cctcgacttt
      601 tccccaaaaa atcttacttt taccgcttga tgacccgggt caaagactct gagccaagtc
      661 gaagagcgga caatccactt aaggcggaca cccctttgat gcagcagtac tggaagatca
      721 aaagccagta cccggaggag attgtctttt tccggatggg cgatttctac gaaatgttcg
      781 gggaggatgc taaatccgcc tctcagattt taggcattgc cctcacctcc cgcgcccacg
      841 gcgatatgga aaaaattccc ttagcgggcg taccgtacca tgcggctgac cgctacctgg
      901 caaagttgat tgccgccggg aaaaaagtcg tcgtctgcga gcagatggaa gatccgaaat
      961 tagccagggg gctggtcaag cgagatgtgg tggaaatcat cacgccggga acggttctgc
     1021 tcgcgaatgc cggtgcggga gagcggagca cttacttggt gagcctctat ccgtcttccg
     1081 atggtttcgg cttagcgcaa ttggaagtga gcacagggga gttccgggtg gaggagggga
     1141 agccggagcg gatcaatgaa ctcctctccg tcctggctcc ggcggaaatt cttttgccgt
     1201 cttctgccaa agaacatcag attaccagga atcacttccc ctggcagccg cagttttcgt
     1261 atcttgacga ttggaaattc gcttttgatt tcgcctaccg cagtttgacc gagcacttcg
     1321 ggacgcagtc tctggccggt tttggctgtg acggcctgaa agtgggaatt tctgcggcag
     1381 gagcgactat cgtttatctc aaagaaacca aaaaaaccgt cctctcccac attcgctcgc
     1441 tttcccgcat cggctcgcag tcagaaatgt ttctcgattt cgccacggtg cgtaatctcg
     1501 aactggtgtc atcgctgggc agtgatgagc ggaaatactc acttttcggg gttttgaacc
     1561 gcacccggac cgccatgggt gcgcggctgc tgcgccaatg gctccttgcg cctttgatcg
     1621 aagtcgagcc gattcaaaaa cggcagaaag ccgtcgctcg tttggtgaag gatgccgacc
     1681 tgcgcggaga catccagaat cttttgagcc gcgcttcgga tctggaaagg ttagcaggac
     1741 gattgggagc gagcaaggcc agtccgaaag atttgatcgg catcaaagaa acctgtgcac
     1801 ttttgcctac gtttcagaaa ttgctggcag acgaaaaagt ctcactcctc gctgaactca
     1861 atcaagaact cccggacgtc agcgatgtcg tcgatctcat ctcccgaagc gttgtcgatg
     1921 aaccgccggt agtgaccaac gagggcgggc tgatcaagtc cggctacgac ggcaaactcg
     1981 attctctgaa ggactctatc cggggcagta aaacctggat tgcaaattta caagccattg
     2041 agcgggaaag gactggcata ccttcattaa aggtcgggtt taacaaggtt ttcggctact
     2101 atctggaggt caccaccccg cacgtggcga aagtcccgcc ggattacatc cgcaagcaga
     2161 cgctggtgaa tggcgagcgg tacatcaccc cggaattaaa agagaaagaa gaactgattc
     2221 tcaacgccga agaaaaaatc aaccgcctcg agcaggaact gtttctggtc atctgccagc
     2281 gcgtcgccca gcgcatcggc gatctccaaa gggcggcctc ggtcctggcg cagctggatg
     2341 tactcgcctc tctcgcccac gtcgggtggg aaaacggcta ctgtttaccc gaggtcaaca
     2401 ccgagggcca gatagaaatc accgaaggcc ggcaccccat catcgaaaaa gtcttgccca
     2461 aagggagttt tgttcccaac gacacgcaaa tcgacttaga gcacggcatc attcaaatca
     2521 tcaccgggcc gaatatggcg gggaaatcga cctacctccg gcaggtgggg ctgattgtgc
     2581 tcctctcgca gatcggctcg tttgtcccgg cggaaaaggc cagaatcggc atctgcgacc
     2641 gcatcttcac ccgcgtcggc gcggcggatg atttgatcgg cgggcgctcg acttttttgg
     2701 tcgaaatgaa tgaaacggcc aacatcctga acaacgccac gcccaaaagt ttgattctcc
     2761 tcgacgaaat cggccggggc acctccacct acgacgggct ttcgattgcc tgggccgtgg
     2821 cggagtatct gcacgacagc cccaaactcg gcgcgcggac gctctttgcc acccactacc
     2881 acgagttgac cgagctggcc tccatcttgc cgaaggtgaa aaattatcag gttgccgtaa
     2941 aagagtcgaa gaccctgtct tcgaccctga gcgaagccga agggagcgaa gtcgaggggt
     3001 ggcaagatca aatcattttc ctgcgcaaga ttgtgcccgg cgggtgcgac gactccttcg
     3061 gcattcaggt ggccaaactc tccggcatcc cggaagcggt gttgacccgg gcgaaggagg
     3121 tgttagattc tctggagaaa gaagagtcgg tgcctaagaa aaacgaaaag cgaaaactgg
     3181 tcttgaaagc gccgcaacca acactttttg agaaaaccga tccgggagta tatgactaaa
     3241 aaaattcgca gctttttcta tactggcaaa ataccaatta ctttttttcg atttgctgta
     3301 atactgcttc caattcagag cgtgagtgcc caaaattttt gggagcaggc caatggaccc
     3361 tatagcggat ggattcaagc tcttgcgatt aattcaagcg ggcacttctt tgccgggact
     3421 tatggtgccg gcgtttttcg ctcgattgac aatggcggta gctggacaca aaccggcttg
     3481 acggtttcca ctgtctggtc tcttgccatc aattcgagcg gggcgatctt tgccgggact
     3541 cgcggcggtg tctttcgctc cactgacaat ggcaatagct ggacacaaac cggcttggcg
     3601 gatgtctact gtcttgcgat caattcgagc gggcagatct ttgccgggag ggccaatgca
     3661 ggtgtctttc gctccactga caatggcggt agctggacac aaaccggctt gatgaatacc
     3721 ttcgtt
//
LOCUS       DBNGZA010000519         3423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_208232, whole genome shotgun sequence.
ACCESSION   DBNGZA010000519 DBNGZA010000000
VERSION     DBNGZA010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_208232"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16FU_11010"
     CDS             <1..264
                     /locus_tag="AC16FU_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR2994937.1"
                     /translation="MLPKAAVFAQSQAEVVAHNIACQIKGSGNPYFFDGVGACYLETG
                     YGKAGYAMGNFFVEPRPQVVMKKSGRLWHWAKVRFERRWRKSR"
     gene            431..1567
                     /locus_tag="AC16FU_11015"
     CDS             431..1567
                     /locus_tag="AC16FU_11015"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR2994938.1"
                     /translation="MRFETKVIHAGQEPDPATGAIATPIYQTSTYVQEGIGKHKGYEY
                     SRTQNPTRTALEKSLAALEGGKYALAFSSGMAAINTVLNLLRTGSHVISSDDVYGGTY
                     RIFEQVYRQYGLEFDYVNTSNISEIKKAIKSNTRLIWVETPTNPLLKISDLAEISRLT
                     KQRQLVLGVDNTFASPYFQQPLALGADIVVHSSTKFLGGHADVVGGAIITSNATFYER
                     LKFCQNAVGAVPGPLDCFLVLRGIKTLALRMRQQERNAEKVVKFLSRHPKVKKIYYPG
                     LKDHPGHLIAEIQMTGFSSMVSFEVSGGLEFARKVCENTQLFKLAESLGGVESLIEHP
                     ALMTHASVPKEVREEKGVSDTLVRLSVGIEHPDDLIADLKQALE"
     gene            complement(1659..2564)
                     /locus_tag="AC16FU_11020"
     CDS             complement(1659..2564)
                     /locus_tag="AC16FU_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994939.1"
                     /translation="MKSKLGLVVLAVTLVFFLFSGCSKSPTSPQTTQLTADQLAVQNL
                     VQTDPAFDVTAAMDESDSVSEGGLAKSAAVLDTVFHWWRQITDRDRHIDIMITDSTAR
                     VTVTDTLMGLLHVVGADSVGRVQFTTPLTDVATHKALFKRRHPPDGDEHRGWVLAAVS
                     DVLFKSSPLDTRQIRTTQVRTSMSGVNRTLTESGITALTPRDSILTFTIGDTVWVTVT
                     TGDPSDSVFLHVRDGHRDMRPHRKSLHNNGDGTFSGQFLVPQDLEGASGRKHMVVDVI
                     KHDVLDNHARYDSHGWGILYRVRNI"
     regulatory      2838..2945
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3154..>3423
                     /locus_tag="AC16FU_11025"
     CDS             3154..>3423
                     /locus_tag="AC16FU_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013644858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR2994940.1"
                     /translation="MTQFARATESQITKAILDRFYQDFTNHLSTDVIIVGAGPSGLVA
                     GRDLAREGFKVLIVESNNYLGGGFWIGGYLMNGVTVRQPAQAILDE"
BASE COUNT          742 a          991 c          984 g          706 t
ORIGIN      
        1 atgctcccca aggccgcggt ttttgcccag agccaggccg aagtggtggc ccacaacatt
       61 gcctgccaga tcaaaggctc aggcaacccc tacttcttcg acggcgtcgg cgcctgctac
      121 ctggaaaccg gctacggcaa ggcgggctat gccatgggta atttcttcgt tgagccgcgc
      181 ccgcaggtcg tgatgaagaa atccggccgc ctctggcact gggcaaaggt tagattcgag
      241 cgacgctggc ggaagagcag gtagaaaaag taaatccagt ggtggcgctt aagtgccgcc
      301 tatgtgttta ggagagcgct ttctcccaat cacgacggtt gcaagaatcc cagcttttcg
      361 catattcagt tccacgctat ggttatcgat gcatgagaaa tagcagaaga attattggag
      421 tgaggtttta atgcgctttg agaccaaggt aatccacgcc gggcaggagc ccgatcccgc
      481 cacgggcgcc atcgccacgc cgatctacca gacctccacc tacgttcagg aggggatcgg
      541 caagcacaaa gggtacgagt actcccgcac ccagaatccc acccgcaccg ctttggagaa
      601 atcgctggcc gcactggaag gcggcaagta cgcactcgcg ttttcctccg gtatggcggc
      661 catcaacacc gtcctgaatc tcttgcgcac cggcagccac gtcatctcct ccgacgacgt
      721 ttacggcggc acgtaccgaa ttttcgagca ggtctaccgc cagtacgggc tggagttcga
      781 ctacgttaat acctcgaaca tctccgagat taaaaaggca attaaatcga atacccgttt
      841 gatctgggtc gaaacgccga ccaacccgct tttgaaaatc tccgacctgg ctgaaatttc
      901 ccgcctgacc aagcagcgtc aactggtttt aggggtcgat aacaccttcg cctcgcctta
      961 cttccagcag ccgttggctc tcggggctga catcgtcgtc cacagttcga ccaaattcct
     1021 gggcggccat gccgacgtgg tgggcggcgc catcatcacc tccaatgcca ctttctatga
     1081 gcggctgaag ttctgccaga atgcggtggg cgcggtgccg gggcctctgg actgctttct
     1141 cgttttgcgc ggcatcaaaa ctctcgctct ccggatgcgc cagcaggaac gcaacgcgga
     1201 gaaggtggtg aaattcctct cccggcaccc gaaggtgaaa aaaatctact atcccggatt
     1261 gaaagaccac cccggacatc tgatcgccga aatccagatg accggcttca gctcgatggt
     1321 ctctttcgaa gtgtccggcg gcctggagtt tgcccgcaag gtctgcgaga acacccaatt
     1381 gttcaaattg gccgagagtc tgggcggggt ggagtcgttg atcgagcacc cggctttgat
     1441 gacccacgct tccgttccca aagaagtgcg ggaagaaaaa ggggtgtccg atacgttggt
     1501 ccgcctctcg gtgggcattg aacacccgga cgatttgata gccgacctca agcaggcgct
     1561 ggagtaaaaa cggtcaaaca ccggactgcg ggaagcgaaa cgggacacac actggtgtgt
     1621 gtcccgtttc ggcttgcccc gcttgacgct agcgcggctt agatattccg gacgcggtag
     1681 aggattcccc acccgtggct atcgtagcgc gcgtggttat cgaggacgtc gtgcttgatc
     1741 acgtccacca ccatgtgctt gcggcccgag gcaccctcca ggtcctgggg aaccaggaac
     1801 tgtccgctga aagtgccgtc cccgttgttg tggagcgact tccggtgcgg acgcatatcc
     1861 cggtgtccgt cgcgcacatg cagaaaaacc gaatcgctgg ggtcaccagt ggtaaccgtg
     1921 acccagaccg tgtcgccgat ggtaaaagtc aagattgagt cgcgcggggt cagcgcggta
     1981 atgccacttt ccgttagggt ccggttcacg ccgctcatac tggtgcgaac ctgggttgtg
     2041 cgaatctgac gcgtatcgag cgggctggat ttaaacagaa cgtcggaaac cgccgcgagc
     2101 acccagccgc ggtgctcgtc accatccggg gggtgacgcc gcttgaagag ggccttatgg
     2161 gtcgccacgt ccgtgagggg ggtggtaaat tgcaccctgc cgacggaatc ggcacccacc
     2221 acgtggagca gtcccatcaa ggtgtcggtg acggtaaccc tggcggttga atcggtaatc
     2281 atgatgtcga tgtggcggtc gcggtcggtt atttgccgcc accagtggaa tacagtgtcc
     2341 agcacggcgg cacttttggc caggccgcct tcgctgacag aatcgctttc atccatcgcg
     2401 gcggtaacat cgaacgccgg atctgtctgc actaaattct gtacggccag ctgatcggcc
     2461 gtgagctgcg tggtttgggg cgatgtcgga cttttgctgc agccggagaa caggaagaac
     2521 accaacgtga ccgccagtac caccaacccg agtttggact tcacctgcaa ctgcattgtt
     2581 ggttacctcc ttacgacttc ttttcgactg aacgacgtac ttaccgtggc cttaagcctc
     2641 gccaggtgca tcggtacctc cttcctttca ccccaattcg cggcgtttgg ccaaaatgcc
     2701 tgctctgact ttcaaaaggg ataaacgccc attgcccggg aatgtttgca ggattttcgg
     2761 ggactggggc gcggtgcaat cctaacccta aaaagtgctt gacaagtttc cgaaagctac
     2821 gtatatagtt gaatagaaaa caaaaccagg ggtggttttc ccgccgcggg cggaggaagc
     2881 ctgagataat acccttagaa cctgaactgg gtaatgccag cgtagggagc gatgatggaa
     2941 gaccgcacca cagtattctg caggaccctc cggttgcggc ctttttattt tcgggtcaac
     3001 cccgccatat ccggggcctg acaaccctcc agttcggtga cctcccttcg ggggcgccgg
     3061 acatccatcg tcaaactcgc gctggcattg ccctgaagac gcagtcccgc caaaaacggg
     3121 atctcgtagt aaacctgaag gaggcctgaa aaaatgacac aattcgcacg ggcaaccgaa
     3181 agccagatca ccaaggcgat tctcgaccgc ttctaccaag atttcaccaa ccacctttcg
     3241 accgacgtga tcatcgtcgg ggctggaccc tcgggactgg tagcaggacg ggatttggcc
     3301 cgggagggtt tcaaagtgct gatcgtggag agcaacaact acttgggcgg cgggttctgg
     3361 atcggcggct acctgatgaa cggggtgacc gtccgccagc cggcgcaagc gattctggac
     3421 gaa
//
LOCUS       DBNGZA010000520         1060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_952196, whole genome shotgun sequence.
ACCESSION   DBNGZA010000520 DBNGZA010000000
VERSION     DBNGZA010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_952196"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16FU_11030"
     CDS             <1..225
                     /locus_tag="AC16FU_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994941.1"
                     /translation="QVNLAIRLALLKVLSEREKPVVILDEPFAAFDAEREKQALALLE
                     KLAEEYQIFLFTCRPVPDRWPARGSLVKLG"
     gene            300..815
                     /locus_tag="AC16FU_11035"
     CDS             300..815
                     /locus_tag="AC16FU_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR2994942.1"
                     /translation="MERIWAPWRKAFILKTRESGCLFCELAAKREDKKSLVVFRGRRV
                     YVVLNRYPYTNGHLMVVPYRHVAELGGITSDEEKEVWALARRSVQLLRQVYQPQGFNL
                     GLNLGRAAGAGVVDHLHLHVVPRWAGDNNFMPVIGNTKIHSLSLDDTFRTLARGFQQK
                     RLPPQKTRKQR"
     gene            812..>1060
                     /locus_tag="AC16FU_11040"
     CDS             812..>1060
                     /locus_tag="AC16FU_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR2994943.1"
                     /translation="MKIPKKKDLLRLQAELKSDRKIAEALGGVPQHLIGYWRLKKNIP
                     LRGGPRFTRKEIEFLWEKHGNDRKASSELGISRAAFYRW"
BASE COUNT          220 a          314 c          333 g          193 t
ORIGIN      
        1 caggtcaact tagccatccg gctggctctg ctcaaggtac tctccgagcg cgagaagccg
       61 gtggtgattt tggacgaacc gttcgccgcc tttgatgcgg aacgggaaaa gcaggccctg
      121 gccctgctgg agaaacttgc cgaagagtat cagattttcc tttttacctg ccgaccggtg
      181 ccggaccggt ggcctgcccg aggcagtctg gtgaaactgg gctgagtccc gcccatacgg
      241 gccgtatggc cgtaggcggg attggcgggt cgaccgcggc gggatatatt gccgggtcaa
      301 tggaacgaat ctgggccccc tggcgcaagg ctttcatcct caaaaccaga gaatctggct
      361 gtctcttctg cgaattggca gccaagcggg aggacaaaaa gagtctggtc gtgttccggg
      421 gccggcgggt ctacgtcgtt ttgaaccgct acccctacac caacggccac ctgatggtgg
      481 tgccgtatcg ccatgtggcc gaactggggg ggattacctc cgacgaagaa aaagaagtct
      541 gggcactggc ccggcggagc gtccagcttt tgcggcaggt ctaccagccc cagggattca
      601 acctcgggct gaatctgggc cgggccgccg gcgccggcgt ggtcgaccac ctgcacctgc
      661 atgtggtgcc gcgatgggcc ggggacaata atttcatgcc ggtcatcggg aacaccaaaa
      721 tccactccct ttccctggac gacaccttcc gcaccctggc gcgtggtttt cagcaaaagc
      781 gtttaccgcc gcagaagacc cgcaagcagc gatgaagatc cccaagaaaa aagacctcct
      841 ccggctgcag gccgaactga aaagcgaccg caagatcgcc gaggccctgg ggggcgtgcc
      901 gcagcatttg atcggctact ggcggctgaa aaagaacatc cccctgcgcg gcggaccccg
      961 ctttacccgg aaggagatag agtttctctg ggaaaagcac gggaacgacc ggaaagccag
     1021 cagtgaactg ggtatttcgc gggccgcatt ctatcgctgg
//
LOCUS       DBNGZA010000521         2931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1059609, whole genome shotgun sequence.
ACCESSION   DBNGZA010000521 DBNGZA010000000
VERSION     DBNGZA010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1059609"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16FU_11045"
     CDS             <1..1098
                     /locus_tag="AC16FU_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR2994944.1"
                     /translation="KGKGYVPAEHDPAKFHGVGAFDKVTGKANAAGETAYQAVFGETL
                     VKIAEKNRKVVAITAAMCTGTGLVEFAKKFPERFFDVGIAEEHGATFAAGLACQGIKP
                     VYAIYSTFLQRAFDQVVHDVALQNIPVLFAVDRAGLVGDDGPTHHGVFDLSYLRAIPN
                     FTVAAPKDGNEFKDLLWSAFDWQDGPVAIRYPRGGIPDKLQDAVRPIPYGTWQVEREG
                     EEVVIFAVGAMVYPALEAATRLLVDGLSPTVVNARFIKPLDEKLLALLLENHKKVITV
                     EDNVLAGGFGSAILEWIEAAGKTGFTVKRLGIPDQFITHGPRKLLLNQLGLDADGLVR
                     TVRALVPAKVPKQTLRLARTLPEAVPPAPQP"
     gene            1101..1979
                     /locus_tag="AC16FU_11050"
     CDS             1101..1979
                     /locus_tag="AC16FU_11050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR2994945.1"
                     /translation="MRLGINANVERPDCETALRRVLRWAAEHKISFSIAEHLVPLVGH
                     PEAACPLPLLPEKSDHILSLGGDGTFLATARAVAARGTPVLGLNVGGFGFLTTISVDN
                     LEKNLDRVRSRAYLLEERMVLEGAIEDAGAPAFALNDVVVEKGTTARLIHLALYVNGE
                     YVSSYSADGLIVATPTGSTAYSLSVGGPIINPKINAIIVAPVSPHALTSRPIVFSEKD
                     EIRIVLAGVRQKAAVSLDGQVNFELLPGQAVAVKKAPHTLKVVTFPETSFYDVLRRKF
                     HWGGIPAAGKQAVTEP"
     gene            1986..2825
                     /gene="mtgA"
                     /locus_tag="AC16FU_11055"
     CDS             1986..2825
                     /gene="mtgA"
                     /locus_tag="AC16FU_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR2994946.1"
                     /translation="MRFFATRRGKIWGLSGGLAGLVLLWALAEYLSVPDVSALARQNP
                     ETTALIERRQAEYRARKEKVERCQFWVPFSQISPYLKRAVLVAEDISFYEHGGYDLAE
                     IKRAIKINLKEKRFARGASTLTQQLAKNLYLSTSKNPFRKLRELFIALALEKHLSKRR
                     IFELYLNNIEWGRGLYGVEAAALNYFDKAAADLSPDEAARLAAMIPDPLHLTPFDSSR
                     HFERKRRLILTRMEQYGFLTHEEYTQAVAADARGLNPGEGPTDNPAFEAKATDDTLSE
                     QGR"
BASE COUNT          570 a          894 c          889 g          578 t
ORIGIN      
        1 aaaggcaagg gctatgttcc ggcagaacat gatccggcga agttccacgg cgtgggggcc
       61 ttcgataagg tgaccggcaa agcgaatgcc gcgggcgaaa cagcctacca ggcggtcttc
      121 ggcgagacgc tggtcaaaat cgccgagaaa aatcggaagg tggtggcgat caccgccgct
      181 atgtgcaccg gtacggggtt ggtcgaattc gccaaaaagt ttccggagcg tttctttgac
      241 gtgggcattg ccgaagagca cggggcgacg tttgccgccg gcctggcctg ccagggaata
      301 aaaccggtct atgccatcta ctcgaccttt ctgcagcggg cgtttgacca ggtcgtccat
      361 gatgtggccc tgcagaatat cccggtcttg tttgcggtcg atcgcgccgg tttagtcggc
      421 gacgacggcc ccacgcacca cggggtcttc gatctctctt acctgcgggc gatccccaat
      481 ttcaccgtgg ccgcgccgaa ggacggcaat gaattcaaag acctgctctg gtcggctttt
      541 gactggcaag acggcccggt ggccatccgc tacccgcgcg gcgggatacc cgataaactt
      601 caggatgcgg tccgcccgat tccctacggc acctggcagg tagagcgcga gggggaagag
      661 gtggtaattt tcgccgtcgg cgcaatggtg tatccggcgc tggaagccgc cacgcggcta
      721 ttagtagatg gtctttctcc gaccgtggtg aatgcccgct tcatcaagcc cctggacgag
      781 aaactcctgg cacttctcct ggagaaccac aagaaagtca tcaccgtgga ggacaatgta
      841 ctggcgggcg gattcggttc ggccattctc gaatggatcg aagccgcagg caaaaccggc
      901 tttaccgtca aacgtctggg gattcccgac cagttcatca cccacggccc gcgcaagctg
      961 ctcttaaacc agcttggcct ggatgccgac ggtctcgtcc ggaccgtcag agcgctcgtg
     1021 cctgccaaag ttccgaagca gacgttgcgc ctggcgcgga cgctgcccga agcggtcccc
     1081 cctgctcccc aaccctaacg atgcgtctgg ggattaatgc caacgttgag cgcccggact
     1141 gcgagaccgc cctgcggcgc gttttgcgct gggccgccga acataaaatc tccttctcca
     1201 tagctgagca cctggtcccc ctggtggggc acccggaggc ggcctgtccg ctccccctgt
     1261 tacccgaaaa gagcgatcat atcctctctc tgggcggaga tgggactttt ctggccacgg
     1321 cccgggcggt ggctgcccgg ggcacgccgg ttttgggcct gaacgtcggc gggttcggct
     1381 ttctcacgac gatttcagtg gacaatttgg aaaaaaatct cgaccgggtg cgctcccgag
     1441 cttacctctt agaagagcgg atggtgctgg agggggcgat tgaggacgcg ggcgcacctg
     1501 cctttgcgct caacgacgtg gtggtggaaa agggcactac cgcccgcttg attcacctcg
     1561 ctttgtacgt gaatggtgag tacgtttcat cctactcggc ggacgggttg atcgtcgcca
     1621 caccgaccgg ctcgacggcc tacagcctct cggtcggcgg gccgatcatt aatccgaaaa
     1681 tcaacgccat catcgtggcg ccggtgagcc cgcacgcgct cacctcgcgg ccgatcgttt
     1741 tttcggaaaa agacgaaatc cggatcgttc tggccggtgt ccggcagaaa gcggcggtga
     1801 gtctggacgg ccaggtgaat tttgaactct tgcccggcca ggcggtggcg gtcaagaagg
     1861 ccccgcacac cctcaaggtc gttacctttc cggaaacctc gttctacgac gtgctccggc
     1921 gcaagttcca ctggggcgga attcccgccg ccgggaagca ggccgttacc gaaccgtaag
     1981 cggccatgcg ctttttcgcc acgcgccgcg gcaaaatctg gggcctgtcg ggcggcttgg
     2041 ccggactcgt tctcctgtgg gctctggccg agtatctctc cgtcccggac gtttcggcgc
     2101 tcgcccggca gaatccggag accaccgctc tgatcgaacg gcgccaggcg gagtatcgtg
     2161 cgcggaaaga aaaggtggag cgctgccagt tctgggtccc cttcagccag atttctccct
     2221 acctcaagcg ggcggtcctg gtggccgaag atatttcctt ctacgaacac ggcgggtacg
     2281 acttagccga gatcaagcgg gcgatcaaga ttaacctgaa ggaaaagcgc ttcgcgcgcg
     2341 gcgcctctac cctcacccag cagttagcca agaacttgta tctctccact tcaaaaaatc
     2401 ccttccgcaa actgcgcgag ttgttcatcg ccctggccct ggagaagcac ctttccaagc
     2461 gccgcatttt tgaactgtat ttgaacaata tcgagtgggg gagaggcctt tacggcgttg
     2521 aggccgccgc gctcaactac ttcgacaaag cggcggccga cctctcgccg gacgaagcgg
     2581 cccggctggc cgccatgatc cccgatccgc tgcacctcac tcccttcgac tcttcccggc
     2641 acttcgagcg gaagcgcagg ttaattctaa cgcggatgga acagtacggc tttctgactc
     2701 atgaagagta cacacaggcg gtcgctgcgg acgcccgggg attaaacccg ggcgaaggtc
     2761 cgaccgataa tcccgctttt gaagcaaaag cgacggacga cactctctcg gagcaagggc
     2821 gatagatcgg gtcggtgtgc gcacggcgaa aattacgctc tggcagaagg aggcgaatgg
     2881 aaatttttcc ccgagtattc gctctgagat ttcgagtacc cggccggatt t
//
LOCUS       DBNGZA010000522         4245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1272079, whole genome shotgun sequence.
ACCESSION   DBNGZA010000522 DBNGZA010000000
VERSION     DBNGZA010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1272079"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1637
                     /gene="porX"
                     /locus_tag="AC16FU_11060"
     CDS             60..1637
                     /gene="porX"
                     /locus_tag="AC16FU_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS response regulator signal transducer PorX"
                     /protein_id="HIR2994947.1"
                     /translation="MAVKDDIKDILWVDDEIDQLQSHIIFLEGKGYRVAKAANGDDAV
                     LLVSKNEYDLVLLDEQMPGKDGLTTLEEIKEKKPGLPVIMVTKSEEERLMNEAIGEKI
                     DDYLTKPVNPSQILSAAKRILETKKIRSGHLTQKYIADFNQLRSSLSTQMSWNDWIEA
                     HRRLSEWELELDNFRDLGLEQTHKGQKKEFNLEFSKYVEKNYPLWVKSASGGDAPLLS
                     TDVVKKFLAPHLVAGKQTVFIVIDCMRLDQWLTLESILHEYFTVERAQYFSILPTATP
                     FSRNAIFSGLFPESFPQLDPDLWKPGHDDEFSRNRKERQYLDAQLARLGIRLKGEAKY
                     VKVLDPDEAASVAKKIGAYASTPLLSIVYNFVDMLTHGRAESEILQELAPTESAFRSV
                     MKSWFLHSSLFDVLKYLARQDGVVVITTDHGSIQAMRGSLVHGKRDTSSSLRYKYGDN
                     LNCDTRQAVLLKDPKTYRLPSFTIATTYVLAKEDYYFLYPTNFNEYQQKYQNSFQHGG
                     ISMDEMILPVATLKPKK"
     gene            1752..2198
                     /gene="tsaE"
                     /locus_tag="AC16FU_11065"
     CDS             1752..2198
                     /gene="tsaE"
                     /locus_tag="AC16FU_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR2994948.1"
                     /translation="MTIQLTVVTRKEILSNSLEQTQAYGREFAVGLKSGDVAALSGPL
                     GSGKTAFVRGVCAGLGVTTPVTSPSFIIVNEYPGRLPVYHWDLYRLNSLDEVRRLGIE
                     ECFASEGVCLVEWAEKILSLLPERRWEIHFSIAGENQRKISFQQFS"
     gene            2341..3024
                     /gene="tsaB"
                     /locus_tag="AC16FU_11070"
     CDS             2341..3024
                     /gene="tsaB"
                     /locus_tag="AC16FU_11070"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR2994949.1"
                     /translation="MLVLGLDASSAKLSVGLVDDERTLAEATVELQLSRSEVLLQTLE
                     TCLAWAQVKWQDLDGLAVPVGPGSFTGLRIAMATVKGLAYALGKPVVTCTTFEAIASG
                     FRGISRPLVVLFPAPKETVLVAVYESENGMMHPTLAPQICALDNVTMLFPYGALVTGP
                     GAVRYRNRLEKEYGGSFQLTFESDSFVRGVEVARLGLKRLQAGEVADLAWLEPQYFFA
                     WGSGDAGNA"
     gene            3008..3496
                     /gene="rimI"
                     /locus_tag="AC16FU_11075"
     CDS             3008..3496
                     /gene="rimI"
                     /locus_tag="AC16FU_11075"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR2994950.1"
                     /translation="MLETLKQPAACSIEPMQSADLAAVAQIERDSFSDPWTPANFSEG
                     LNWEQSFFRVARQDGEVVGYLCTLIAAGEMHIANVAVGESRRGLGIGRALVEEGLNEA
                     RRRGCLAVWLDVRQSNRQARALYEQLGFRRVLVRQKYYRKPVEDAWVYCLVLSPRPGE
                     ET"
     gene            3504..>4245
                     /gene="accD"
                     /locus_tag="AC16FU_11080"
     CDS             3504..>4245
                     /gene="accD"
                     /locus_tag="AC16FU_11080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR2994951.1"
                     /translation="MEWFKKAREGLQSQEKKEIPHGLWTKCEACGEIIYVKELEKNFW
                     VCPKCNYHFRIRSKDYIDLLLDADTFKECDDNLISVDPLRFRDSKRYADRTREARRKT
                     QLNDALVCGIGKIDGREVSFAVMDFAFIGGSMGSVVGEKVARTIERSLAYTYPLVIVS
                     CSGGARMQEGILSLMQMAKTSALLAQISEKKIPYLSVLTNPTTAGVMASYASLGDVIL
                     AEPKALLGFAGPRVIQQTIGQELPEGFQS"
BASE COUNT          940 a         1144 c         1273 g          888 t
ORIGIN      
        1 agatgatatt atctgaggct cgcgtagata atctcaatgg aaaacccgag gagtaatctg
       61 tggcggtaaa agacgacatt aaggacattc tctgggtcga tgacgagatc gaccagttgc
      121 agtcgcacat catcttctta gagggcaagg gctaccgggt ggccaaagcg gccaacgggg
      181 atgatgcggt gcttctggtt tccaaaaacg agtacgactt ggttctcttg gatgagcaga
      241 tgccaggcaa agacggcctg accacactgg aggagatcaa ggagaaaaag ccgggcctgc
      301 cggtcatcat ggtgaccaaa agcgaagaag agcgcttgat gaacgaggcc attggcgaga
      361 agatcgacga ctacctgacc aagccggtca accccagcca gattctctcc gcggccaaac
      421 ggattctgga gactaagaaa atccgctccg ggcacctgac ccaaaagtac atcgccgatt
      481 tcaaccagct gcgctcgtcg ctctctacac agatgtcctg gaacgactgg atcgaggccc
      541 accggcggct ctccgaatgg gaattagaat tggacaactt ccgcgacttg gggctggagc
      601 agacgcacaa agggcagaag aaggagttca acctcgagtt ctccaagtat gttgagaaaa
      661 attacccgct ctgggtcaag tccgcctcag gcggagacgc gcccctgctt tccaccgacg
      721 tggtcaagaa atttttggcc ccgcatctgg tggcgggtaa gcaaacggtc ttcatcgtca
      781 tcgactgcat gcggctggac cagtggctga ccttagagtc aatcctgcac gaatacttca
      841 ccgtcgagcg cgcgcagtac ttttcaattt tgcccaccgc cacccctttt tcgcgcaacg
      901 ccattttcag cgggcttttt ccggaatcat tcccgcaact cgaccccgac ctctggaaac
      961 ccggacacga cgacgaattc tcccgcaacc gcaaggagcg gcagtatctc gatgcgcagc
     1021 tggcccggct gggtatccgg ctaaaagggg aggcgaagta cgtcaaggtg ctcgatccgg
     1081 acgaagcggc ctcggtggcc aaaaagatcg gcgcctacgc cagcacgcct ctgctttcca
     1141 tcgtctacaa cttcgtcgat atgctgaccc acggccgggc ggagtcggaa attctccagg
     1201 aactggcgcc caccgagtcg gccttccgct cggtgatgaa atcctggttc ctgcactcgt
     1261 cgctcttcga cgtgctcaag tacctcgccc gccaggacgg ggtggtggtc atcaccaccg
     1321 accacggctc gatccaggcg atgcgcggat ctctggtgca cggcaagcgc gacacctcgt
     1381 ccagtctgcg ctataagtac ggcgacaact tgaactgcga cacccggcag gcggtgctcc
     1441 tcaaagaccc caaaacctac cggctgccgt cgttcaccat cgccaccacg tacgttctgg
     1501 ccaaagaaga ttattacttc ctttacccga ccaacttcaa cgagtaccag cagaagtacc
     1561 agaactcctt ccagcacggc gggatttcga tggacgagat gattctgccg gtggcgacgc
     1621 tcaagccaaa aaaataaagg cgtgtcgaga tgtcgaagtt taggggtgtc ggagttgact
     1681 ccgaccagtt gctccttttc caaaagccag cgattatacc tctgctgaaa taaagaaacg
     1741 gtccgaaaga cgtgactatt caactaacgg tcgttaccag aaaggagatt ctttcgaatt
     1801 cgctggagca gacgcaagcg tacgggcggg agtttgcggt gggcctgaag tccggggacg
     1861 tggcagcact cagcgggcct ctggggagcg gcaagacggc gttcgtgcgg ggagtctgcg
     1921 cggggctggg agtgaccaca ccggtcacca gccccagttt catcatcgtc aatgaatatc
     1981 ccgggcggct gccggtttac cactgggacc tctatcggct caattccctt gatgaagtcc
     2041 gccggttggg gatcgaagag tgctttgcct cggaaggcgt ttgcctggtg gaatgggcgg
     2101 agaaaattct ttctctcttg cccgagcgga ggtgggaaat ccacttctcg atcgctgggg
     2161 aaaaccagag gaagatttca tttcaacaat tttcctgagg gaaatagagg caaagagaag
     2221 acggtcaggc gaccgtcgct gccccacgta ggggcataac aggcaaacct tgtcttttac
     2281 gaacgatgta aatatgttta tattttggca caagaaatat tgaaagtggg tgttactgtc
     2341 atgctcgttt taggattaga tgcttcaagc gcgaaacttt cggtgggttt ggtagatgac
     2401 gaacggacgc tggctgaagc cacggtggag ttgcaacttt cccgatcgga agtattgctc
     2461 cagacactgg agacctgctt ggcctgggca caggtgaaat ggcaggacct ggacggactg
     2521 gcggttccgg taggccccgg ctctttcacg ggactgcgca tcgctatggc cacggtcaaa
     2581 ggactggcat atgctctggg aaaaccggtg gtgacctgta ccacgtttga agcaatcgct
     2641 tctggttttc gcggcatttc ccggcctttg gtcgtgctct ttcccgcacc gaaagagacg
     2701 gtgctggtgg ccgtctatga atctgaaaac ggtatgatgc accccacgct ggcaccgcag
     2761 atttgcgcgc tggataacgt caccatgctt tttccctacg gagcactggt gaccggcccg
     2821 ggagcggtgc ggtatcgtaa ccggctcgaa aaagaatacg gcggaagttt tcaactcacc
     2881 tttgagagcg attcattcgt gcggggggta gaggtggccc gtctggggtt aaaaaggctg
     2941 caagccgggg aggtcgctga tctcgcctgg ctggagccgc aatacttttt tgcctggggt
     3001 tcgggtgatg ctggaaacgc ttaagcagcc ggcggcttgt tccatcgaac cgatgcagtc
     3061 tgccgacctg gcggcggtgg cccaaatcga gcgcgactct ttttccgatc cctggacccc
     3121 ggcgaatttc tcagaaggac tgaactggga gcagagtttt ttccgggtag cccggcagga
     3181 tggagaagtc gtgggctacc tctgtaccct catcgccgcg ggggagatgc acattgccaa
     3241 cgtcgccgtg ggggagagcc gccggggttt gggaataggc cgggcgctcg tcgaggaagg
     3301 tttgaacgag gcgcggcggc gggggtgttt ggccgtctgg ctggacgtac ggcaatcgaa
     3361 ccggcaggcc cgggcgctct acgagcagtt aggttttcgg cgggtgctgg tccggcagaa
     3421 gtactaccgc aaaccggtgg aagatgcctg ggtctactgt ctcgtactat cacctcgccc
     3481 gggtgaggaa acgtaggcac gctatggaat ggttcaagaa agcgcgcgaa gggctgcaga
     3541 gccaggagaa aaaggaaatc ccgcacggct tgtggaccaa gtgcgaggcg tgcggggaaa
     3601 tcatttacgt taaggaattg gagaaaaatt tttgggtttg ccccaaatgt aattatcatt
     3661 tccggatccg ttccaaagat tacattgatc tgctcctgga cgccgacacc ttcaaagagt
     3721 gcgatgataa tctgatctcg gtcgatccgc tccgcttccg cgattctaag cgctacgccg
     3781 accgcacccg ggaagcccgg cgcaagaccc agttgaacga cgccctcgtc tgcggcatcg
     3841 gtaaaatcga cggaagggaa gtcagtttcg cggtgatgga tttcgccttc atcggcggca
     3901 gcatggggtc ggtggtgggg gaaaaggtgg cgcggaccat tgagcgctct ctggcttaca
     3961 cctatcctct ggtaatcgtc tcctgctccg gcggggcccg gatgcaggag ggcattctat
     4021 ccttgatgca gatggccaag acctcggcgc ttctggcgca aatctcggag aagaaaattc
     4081 cgtacctgtc ggttttgacc aatcccacca ccgccggggt gatggcgagt tacgcctcgt
     4141 taggtgacgt catcttagcc gagccgaagg cgcttttggg ctttgccggg ccgcgggtca
     4201 ttcagcaaac catcggtcag gaattgccgg agggcttcca gagtt
//
LOCUS       DBNGZA010000523         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Zixiibacteriota bacterium isolate MAG05492
            SRR21617311_k127_1801730, whole genome shotgun sequence.
ACCESSION   DBNGZA010000523 DBNGZA010000000
VERSION     DBNGZA010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414900
            Sequence Read Archive: SRR21617311
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Zixiibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Zixiibacteriota bacterium
            Bacteria; Pseudomonadati.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 23:43:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,176
            Genes (coding)                    :: 2,157
            CDSs (with protein)               :: 2,157
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 1 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 8 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Zixiibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617311_k127_1801730"
                     /isolate="MAG05492"
                     /isolation_source="soil"
                     /db_xref="taxon:2053527"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.66 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="AC16FU_11085"
     CDS             complement(<1..546)
                     /locus_tag="AC16FU_11085"
                     /inference="COORDINATES: protein motif:HMM:NF040068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR2994952.1"
                     /translation="MLVTEGKSIATVSLKEKIEKEFLPFVIKPGRYVGNELGAIRKDP
                     EEKVRFCLAFPDAYELGMSYLGMGILYHLINELPYAWAERVYAPWIDAQELLRRKNIP
                     LFSLESRTPLKSFDAVGFSISYELTYSNILSMLDLAGIPILSSRRDEDDPIILAGGTA
                     VVNPEPVADFIDLFVLGDAEEI"
     gene            complement(665..>3343)
                     /locus_tag="AC16FU_11090"
     CDS             complement(665..>3343)
                     /locus_tag="AC16FU_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR2994953.1"
                     /translation="KAIIERLINDPRIAEAYPELQPQLVEPVGPGEQAPLVPGGTEPG
                     LRAIKADSAWLMGYTGRGRLVCNFDNGVLGTHRAFSTRWRGANGAPASASWFDPTRNS
                     AFPDTFFVGTNWHGTFTMGIMCGRDPVSFDTIGVAINAQWIAAGVIDVSFTPTSYLAL
                     DAFEWAADPDGDPNTIEDVPDVVNNSWGFPRVNPFSCAVDDSSFLIGCSNVFWGAIDN
                     LEAAGCAVVFAAGNEGGCGAGTLRNPANRASTPYNAFSVGATSPLTPFTIASFSSRGP
                     SDCDGSIKPEVVAAGNGVRSSGWPSGPRTNTSYGVSSGTSFSTPYVAGAVALLREFNP
                     NATVDTIKYALLNSATDLGTPGEDNAYGRGMINIKAALGLLPPNDSIHLFVKRDILTD
                     LNGGTAEPGDTLALLLRLGNSGADTSSGTWGRLFSTDPRVTVVAGFDSAFFGTIAPKD
                     SAANATTLLRFAFALTAPLNENLPLKLAVFTNAGPLDTLPLFFHTRDRLVRSIVTHSL
                     ANYFFTVSNFGQFGFGPGSEIERGALGQGFFYPKAGGLNNLFEMALLMGTSASTVSNA
                     ARGDFSSILIDRHFVPIAGGNLTRLTPGPQADEESFARFNDSGAPRPLGVEITQNIMS
                     FTAANYDDFAILEYRIKNVSGGALNGLRVGLFADWDFPWGSGASDSVDFDATRNLGYM
                     MHATIRQPRGLAVLTPPGATSYRAINNPDSIYDGFSMGEKWRFMSQGVAQVRSGGGDR
                     AHLISTGPFNLLPGQTDTVAFAVVGGGNVSALQANTDSARSKYLAITTEVKEREVSAP
                     LPEQFALSQNYPNPFNPNTVIRFALPKASEIELSVYNLLGQKVATLATGLRSAGVHEV
                     VWDGTDAFGHPAASGLYFYRLKAGDFTDARKMLLLK"
BASE COUNT          750 a          934 c         1039 g          620 t
ORIGIN      
        1 gatttcttcg gcgtcaccta aaacgaaaag gtcgataaag tcggccaccg gctccgggtt
       61 gaccaccgcc gtgccgcccg ctaggatgat cggatcgtcc tcatcccgcc gggatgagag
      121 aatcggaatg ccggccaagt cgagcatgga gaggatgttc gagtaggtca gctcgtagga
      181 gatggaaaag ccgaccgcgt cgaaagactt cagcggtgtc ctgctctcca gcgaaaagag
      241 cgggatattt ttcctgcgga gcagttcctg ggcatcgatc cagggggcgt agacccgttc
      301 ggcccaggcg taggggagtt cgttgatcaa atggtagaga atgcccatcc cgaggtacga
      361 catccccagt tcgtaggcgt ccgggaaggc caggcaaaaa cgcaccttct cctccgggtc
      421 cttgcgaatg gcgcccagtt cgttgccgac gtagcgcccc ggcttgatga cgaaagggag
      481 aaactccttt tcgattttct cttttaagga tacggtggcg atgcttttcc cctcggtaac
      541 cagcataagg aagcctgtat tccctccacg gagtgtaaag aaattaccgt tttggtctca
      601 ctttgtaaag cgggcaaaaa gaaaccccgc cgaggcgggg tttccattcc ggcatacggg
      661 cacatcattt caagagcagc atttttctcg cgtcggtgaa atcacccgcc ttgagccggt
      721 agaagtagag gccggaggcc gccggatggc caaaggcgtc ggtaccgtcc cagacaactt
      781 cgtgaacccc agctgatcgc agacccgtgg ccagggtggc cactttttgc cccaaaaggt
      841 tgtagactga gagttcaatc tcgcttgctt tgggcaaggc aaagcgaatg accgtgttcg
      901 ggttgaacgg gttggggtag ttctgagaga gggcaaactg ctcaggcaaa ggggcggaga
      961 cttcgcgctc tttcacttcg gtggttatgg ccaggtactt cgaacgggcc gaatcggtat
     1021 tcgcctgaag ggctgacaca ttgccgccac cgacgaccgc aaaggccacc gtgtctgttt
     1081 gaccgggaag caggttgaag gggccggtgg aaatcaggtg ggcgcggtcg cccccgcccg
     1141 agcggacttg cgccaccccc tggctcataa agcgccactt ttcacccatg gaaaagccgt
     1201 cgtaaattga atcggggttg ttaattgccc ggtaggacgt ggcgccgggc ggcgtcagaa
     1261 ccgccagccc gcgcggctgt cggattgtgg cgtgcatcat gtaacccagg ttacgggtgg
     1321 catcaaaatc caccgaatca gaagcacccg aaccccaggg gaaatcccaa tcggcaaaaa
     1381 gcccgacccg caggccgttg agcgcaccgc cggatacgtt tttgatccga tactccagga
     1441 tagcgaagtc atcgtagttg gcagcggtaa aggacatgat gttctgggta atctccaccc
     1501 ccaaaggccg cggcgcgccg gagtcattga atcgggcaaa agactcttcg tccgcctgag
     1561 ggccgggcgt caggcgcgtc agatttccgc cggcaatcgg cacaaaatgg cggtcaatga
     1621 gaatggagct gaagtcaccc cgcgcggcgt tagagacggt gctggcgctg gttcccatta
     1681 aaagcgccat ttcaaacagg ttgttcaatc cgccggcttt ggggtagaaa aacccctggc
     1741 ccagcgcgcc gcgctcgatc tcagaaccag ggccaaagcc aaactgtccg aaattagaaa
     1801 ccgtgaagaa gtagttcgcc agtgagtggg tgacgattga acgcaccaga cggtcccggg
     1861 tatggaagaa taagggcagc gtatccagcg gcccggcatt ggtgaagacg gccaatttca
     1921 aggggagatt ttcgtttaag ggtgcagtca gggcaaaagc gaagcgcaaa agagtcgtcg
     1981 cgttcgcggc gctgtctttg ggggcaatgg tgccgaagaa cgcagagtca aagccggcta
     2041 ctactgttac ccgcggatcg gtcgaaaaga ggcggcccca ggtgccggac gaagtatccg
     2101 cgccggagtt ccccagccgg agcaaaaggg cgagggtatc gcccggctcg gccgtccccc
     2161 cgttcaggtc ggtcaggatg tctcgcttca cgaacaggtg aatgctgtcg ttggggggga
     2221 gcagccccag ggcggcttta atattaatca tgcccctgcc gtacgcgttg tcctcgccgg
     2281 gggtacccag atcagtcgcg gaattcaaaa gggcgtattt aatcgtatcg acggtggcat
     2341 tggggttgaa ttcacgcaag agggccacgg ctccagctac ataaggtgtg gaaaaagaag
     2401 tcccggagga gactccataa gaagtgttag tgcgcggtcc cgaaggccag ccggaagagc
     2461 ggacgccatt ccccgcggcc acgacctccg gctttatgga gccgtcgcag tcggaggggc
     2521 cccggctgga gaaagaggca atcgtaaaag gtgtgagggg agaagtagcc ccgactgaga
     2581 aggcattgta aggagttgag gcgcggttgg ccgggttgcg gagcgtcccc gccccgcagc
     2641 cgccttcgtt gccggccgca aaaaccaccg cacagccggc ggcttcgagg ttgtcgatgg
     2701 caccccagaa gacattgctg caaccaatca agaaactcga atcatcaacc gcgcaactga
     2761 acgggttgac gcgggggaaa ccccaggagt tgttcaccac gtcgggcaca tcttcaatgg
     2821 tgtttgggtc gccgtcggga tcagcggccc actcaaaagc gtccagggcg aggtagctcg
     2881 tgggcgtgaa actgacgtcg atcactcccg ccgctatcca ctgagcattg atagccacac
     2941 caatggtatc gaaggatacc ggatcccggc cgcacataat ccccatcgta aaagtgccgt
     3001 gccagttggt gcctacaaaa aaagtgtccg ggaaggcgga gttccgtgtt gggtcgaacc
     3061 aagaagcgga ggccggcgcg ccgttagctc cccgccagcg ggtgctgaag gcacgatggg
     3121 tgcctaagac tccattatcg aaattacaca ctaagcgtcc gcgaccggtg taacccataa
     3181 gccaggcgga gtcagccttg atcgcccgca gtcccggctc agtcccgccg ggaactaaag
     3241 gagcttgttc cccggggccc accggctcca ctaactgcgg ctgcagttcc gggtaggcct
     3301 cggctatccg gggatcgttg atcaaccgct caataatcgc ctt
//
